LOCUS       DAONUO010000001        23197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_1548_length_23197_cov_3.550514, whole genome shotgun sequence.
ACCESSION   DAONUO010000001 DAONUO010000000
VERSION     DAONUO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 23197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23197
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1548_length_23197_cov_3.550514"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..903
                     /gene="argB"
                     /locus_tag="PK768_00005"
     CDS             43..903
                     /gene="argB"
                     /locus_tag="PK768_00005"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQA59387.1"
                     /translation="MGPLEKAEILIEALPYIKKFYGKTVVIKYGGHAMTDPELKDKVI
                     TDIVLMKYVGINPVIVHGGGPEINQWLKKTETPSEFVNGMRITDEKIMEIVQMVLVGK
                     VNKEIVSLVEKNGGKAMGLSGIDGQMIKARKKRAYLNGSEVDLGFVGEIEEIDPQLIH
                     TALRNDFIPVIAPVGTGNGQVYNINADYVAGSIAASLKADKLVLLTDVEGVLDKEKKL
                     ISSLNFREARNMIEAGVIEGGMIPKIECCMEVLSRGVERVHIIDGRLPHALLLEIFTD
                     QGVGTMVVKE"
     gene            909..2084
                     /locus_tag="PK768_00010"
     CDS             909..2084
                     /locus_tag="PK768_00010"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HQA59388.1"
                     /translation="MENLIALGEKYVMNTYKRLPHVLVKGSGSYVWDSEGRKFLDFVS
                     GIAVNSLGHCPQVVTEAIKEQAEKLIHCSNLYWIEPQIKLAKLLVEKSCCDKAFFCNS
                     GTEANEAAIKLARKWGQGRYEIISMEKSFHGRTLGSLAATGQAKYQEAFKPLPAGFKY
                     VPFNDLEALEAAITPETCAVMLECIQGEGGVNVPEPGYLEGVQEICRKNNLLLILDEV
                     QTGLGRTGKAFAYQHFNLSPDIITLAKALGGGVPIGAMLAKDEVAKAFQPGDHAATFG
                     GNPLATAAALAACSVLLDEEFIVEVAKKGEYLKGELEKLKAEFPQIIKVKGLGLMVGC
                     ELNTDALPIVESCMQKGVLINAVGGNILRFVPALTVTKAEIDQLVSTLKEALSESGK"
     gene            2104..3036
                     /gene="argF"
                     /locus_tag="PK768_00015"
     CDS             2104..3036
                     /gene="argF"
                     /locus_tag="PK768_00015"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQA59389.1"
                     /translation="MDTGLKGRDFLSLKDFSAEEIKFLLDLAAKLKAEHKQGVCHDIL
                     KGKTLAMIFQKASTRTRVSFEVGMFQLGGMAHFLSTQDLQIGRGEPISDTARVLSRYV
                     DGILIRTYAQAEVEELAEYADVPVINGLTDLYHPTQALADMLTILEYKKKLSGIKLAY
                     IGDGNNMVHSLMIGGTKLGLEVRVATPKGYEPLPEVVEMSEAFARESGGKLILGNDPE
                     EISHGADVLYTDVWASMGQEQEAKERKKRFKGFQINKKILELADSEAIVMHCLPAHRG
                     EEITDEVMEGPQSVVFDEAENRLHAHKAILAALL"
     gene            3139..3729
                     /locus_tag="PK768_00020"
     CDS             3139..3729
                     /locus_tag="PK768_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HQA59390.1"
                     /translation="MIAYKLGDSLYLNITNRCTNDCEFCIRQQAPGVGGYNLWLEEEP
                     TTKEIIEAIGDPTGYKEVVFCGYGEPLMRLQVVIDVAKHLKKTYPNVPVRVNTNGQAN
                     MIYGEDVTPQLEGNIDVISISLNAENAEKYSEICHPEYGEDAFFSILEFTRKCKSHIP
                     RVIVTVVDIPGIDIAKCKRLAEELGVEFRLRSLDKR"
     gene            3999..4832
                     /gene="rsmA"
                     /locus_tag="PK768_00025"
     CDS             3999..4832
                     /gene="rsmA"
                     /locus_tag="PK768_00025"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQA59391.1"
                     /translation="MRRYNLRPNKRLGQHFLVDSRPLYAMLQAAELLPEDGVLEIGPG
                     LGVLTRELCSRAGKVVAVEKDRQLIPILEDLTKDFKNICILDADVLKLDMEELREEHF
                     GGSFKVVANLPYYITSPIIMKIIENRHLIPLAVLMVQKEVAQRLVASPGSKDYGILTI
                     AVNMYAEVKLVCNVGKSSFLPPPKVESAVVKILLRQKPRVEVDEELFFKVVEAAFGER
                     RKTLKNSLSRRLDIPGIDPEVIAKALEVTGIDPMRRGETLSIEEYAQLLEALKKYAGE
                     T"
     gene            4936..5406
                     /locus_tag="PK768_00030"
     CDS             4936..5406
                     /locus_tag="PK768_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HQA59392.1"
                     /translation="MIFVNPTRGRMSIKEVVRDIIAFMEEVPSASYKLIIGTDSQSRD
                     NSCFVTAVIVHRVGKGARYYYYRKYVSSVKNLRHKIFTETSMSLDAVTRLKEELAKTR
                     YKDMDVEIHVDIGQNGDTKELIREVVGWVMGVGYKVKIKPNACGATKVADRHTK"
     gene            complement(5459..6247)
                     /locus_tag="PK768_00035"
     CDS             complement(5459..6247)
                     /locus_tag="PK768_00035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA59393.1"
                     /translation="MNMAVVPAKNEQGRIGRVLSLLKQTPVDRIVVVVNGSSDDTMKE
                     VKGLRLENLEILYFTPELGIDIPRAIGAYYAYREGAGCVVFVDGDLIGNIRTLVDELV
                     HSVTCQGADLAMTDCYPSLEYKSELTEQILLFRSLLNLKLDLYDEIGVATPSHGPHAV
                     SRKLMEGIDFRYFAVPPMVLAFARKQGLSVQVPSKLPQKYLGSKIRDYFHAKRIADTI
                     IGDTLEAINYFSGRPRSRCYLYKEYQGYNPFRRFDILESFLSKA"
     gene            6350..7213
                     /gene="yabG"
                     /locus_tag="PK768_00040"
     CDS             6350..7213
                     /gene="yabG"
                     /locus_tag="PK768_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HQA59394.1"
                     /translation="MAKVKIGDVVARVSYNKDVFFKVVDIMEDKGCCTLKGLNVRVLA
                     DAPIDDLILPSSTEVREYKRDYIKKSNESMERIFYRRELEKQRRMTRNNAEGSNGFFD
                     LPGMVLHIDGDEEYLDLCLTTYRQLDIEAYGECIAEKNQPKRINSLLVKYNPDILVIT
                     GHDGLLKEKRDFNRVDNYRNSKYFIEAVRNARRFEPSLDDLVIFAGACQSHYEGLLRA
                     GANFASSPHRVFIHALDPVFIIEKVAYTPINKIISARDVIDATITGIKGIGGVETRGK
                     FREGLPRSPYE"
     gene            7235..8662
                     /gene="spoIID"
                     /locus_tag="PK768_00045"
     CDS             7235..8662
                     /gene="spoIID"
                     /locus_tag="PK768_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HQA59395.1"
                     /translation="MDFDNEELTVKLYHTDQQEARIVGIREYVIGAVAASMPPEFPVE
                     ALKAQAICCRTLAVKRMGIFGGGGCKRHPGFDLCNDPVHCQGFMGIEERQKAWGSRYE
                     EFTEKIATAVEDTAEIILTYNNNPIEAVYHEACGGCTEDSENVWGNKVSYLRRVECIY
                     CKDSPYWNTTKNFSIRELKRRLAINFDDGFSDKREIPGLLENIHSTASGRIKKLKIGD
                     MVFQAEDIKNLLGLSSTKFSWKVSALSFQVMGQGNGLGLCQYGARGMALLGFPVDEIL
                     KFYFTGVELKGLERPTYAKPLIGKTIVIDPGHGGDSGNSGPMGLSEGYVNLEIARVAA
                     EGLVQCGAKVILTRDKNEFVSLSERVRISNENKPDITLSIQQNFFPEEDSGGTESFYY
                     PGDVEGKKLACIIHRELVTILQLRDLGAKPADLFVLRETNNPSVMVHIAAISNPEEER
                     LLSEPQFRQKAAVAIINGIKAYYHE"
     gene            8819..9094
                     /locus_tag="PK768_00050"
     CDS             8819..9094
                     /locus_tag="PK768_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HQA59396.1"
                     /translation="MASADAIKKIRRDVQSHLGKMVKLRANRGRKKTFEKEGVLEQVY
                     PNIFVVKVTESPDYVRRISYSYSDILTDTVELTVLQNDKPQSKCGSA"
     gene            9192..10211
                     /locus_tag="PK768_00055"
     CDS             9192..10211
                     /locus_tag="PK768_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59397.1"
                     /translation="MERISRILRQHEKKLLCLENVVGVGLGYKIIKDKMTNKPAIMVL
                     VREKIADEKLLGRNCVPKVLSNTPTDVVEVGDVKLLNARIERARPAKPGMSIGHYKIT
                     AGTFGALVKDVKTGRLLILSNNHVLANATDGGDGKAVIGDAILQPGGHDGGTSSDVIA
                     HLERFVPIAKNAAESSCPIAQGLEKAINSLLKVIKPDYEINFIKKSTARNLVDAAVAR
                     PVRDEYVSGEIMGLGKISGIQEPKIGMTVQKSGRTTGITSSQIQALNVVIKVMLGPTE
                     EATFYDQILTGPMAQPGDSGSIVVDENLRAVGLLFAGSDQATLINPIVNVLKTLQVEL
                     PGAMH"
     gene            10508..11257
                     /locus_tag="PK768_00060"
     CDS             10508..11257
                     /locus_tag="PK768_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HQA59398.1"
                     /translation="MKQIYKKICVALLLTFMLTLPGIATAATQINVKVTLNGRTQVLE
                     LPIDLESISKNYSTTYRFVYTYKDGKWILTSKEDAGQGQAKPSLPTVPSKPQPQTPAP
                     SPSQPKPETPAPSTPDVKGLTADESKMLELVNQERQKLGLKPLTIDMRLVDISRKKSK
                     DMIDNNYFGHTSPTYGTPFDALKNNGVSYRYAGENLAGAPTVERAHSGLMNSPGHRAN
                     ILNPNFTHIGIGVVEGGPYGKMFTQTFIGVN"
     gene            11518..11808
                     /locus_tag="PK768_00065"
     CDS             11518..11808
                     /locus_tag="PK768_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59399.1"
                     /translation="MKVLIFIVVLAIASWVFYLFIRKAFDMPLDQTKGNSGQVKIKPV
                     GSEEVKRDESLKQGTYEEEVSQQPQPKAEDGCCKAATACRQRDEEEMTQIQE"
     gene            11863..13536
                     /locus_tag="PK768_00070"
     CDS             11863..13536
                     /locus_tag="PK768_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HQA59400.1"
                     /translation="MNFRERRILMGVYLTKELVRVDKVVGEDKTQAIVEGTITLPDGK
                     PDIERVISIDATLDTESLETDILDSKIGKVIVEGEVDVNAIYVADVPEGQPQQPVHFV
                     EGKIDFSFFVKIPGVKKDMDVRVRAKIEHIQHSFDPNRPREIKVRLVVMFFVKVTRRV
                     EIEIVIDATGPKDLQVLKKTLRIEDVIGEARAQNIVKSDVGVPEEKPSIEQILKVKAE
                     VRDVTSKIIKNKIIIEGVLEVGILYVAIAPEGQPLQPVHFMEAEIPFTQFVEIHGADE
                     GMARFIRVEVEHIKGRRKDDRTVTVEAILKIRAKVFETKIIQVVIDIFSPSKELHVEK
                     KRLKLDQVIGEDENEIVIKDTLTVPDEKPDIEQIYKTSCKARVTESRIIDGKVIVEGV
                     LTLETLYVADVPEGEPQQPLHFMEHEIDFTTFVEIPGAEEDMMLDFDVIVEHCSSSAI
                     PNDPRRFEVRAVLGLFAKVTQMVELDIVVHVEEEYVEEEEEEKEEKKEKKEKKDDKPA
                     MTIYIVQRGDTLWNIAKRYNVTVEAIVRANNIANPDSIMPGQQLVIPRM"
     gene            13716..14570
                     /gene="ispE"
                     /locus_tag="PK768_00075"
     CDS             13716..14570
                     /gene="ispE"
                     /locus_tag="PK768_00075"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQA59401.1"
                     /translation="MKKITIEAKAKINLTLDVLYKRPDGYHEVESIMQTVSLKDWIFL
                     KLIPGPAIELISNCQELPRDERNLAYKAARLMMEEYQLDAGMAIHIDKNIPIGAGLAG
                     GSADAAAVILGINEMFQLNRPREELMALGKKIGADVPFCIAGKTALARGIGEKILPLK
                     PLSNFGLVLAKPPYYVSTQEVYSRLDVKTIQKRPDTPAMMEHIEQRDIGEIASGLCNV
                     LEEVTLKLYPELELIKQHLISHGALGSLMSGSGPTVFGLFESARDAERAAEKIGLDGN
                     EIFVCKMQ"
     gene            14594..15295
                     /locus_tag="PK768_00080"
     CDS             14594..15295
                     /locus_tag="PK768_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQA59402.1"
                     /translation="MENNRFPSLKLDNYKPLRDIVFEAIKEAILKGRLKPGERLMEVQ
                     LAEEMGVSRTPVREAIRKLELEGLVVMVPRKGAYVSGLTMKDAAEVFEIRSSLEGLAA
                     ALAAERITDDEIKMLDKILSEITKASEKGDAETVIKKDVEFHQILFNASRNERLAQIV
                     NNLKEQIDRFRIQSFSNPLRVKNVVSEHKAIIDAIKQGDSESAEKLAKAHIYEVEYNV
                     MEMLRKRMSFEEELS"
     gene            15292..16029
                     /locus_tag="PK768_00085"
     CDS             15292..16029
                     /locus_tag="PK768_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQA59403.1"
                     /translation="MKALILAGAEKEGPLAQYGPNKALIKIHGKEMIRYIIDAFKGLD
                     FIDSIAVVGPKESLEPIKGQVDVIVDSAASLADNVLCGVNIFSDDEMVLIATSDIPMI
                     TPEAVRDFVEKSLSMDADFYYPIVSREENEKKYPGIKRTYVKIKDGTFTGGNIVMVRA
                     GKVKECIKNAEAFMTYRKKPWMLARILGLKFILKFLMGTLTIDELQERVEELFGVKAR
                     AIISSYPEIATDVDKDSDLELAARALS"
     gene            16041..16850
                     /gene="purR"
                     /locus_tag="PK768_00090"
     CDS             16041..16850
                     /gene="purR"
                     /locus_tag="PK768_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HQA59404.1"
                     /translation="MRRSERIALISNTLSRNPNKIISLNHFSQWLGAAKSSISEDIDI
                     IKKAYKNSGQGDIATIPGAAGGVRYRAKRSLDDINAFLHDLSEVLRDQKRIIPGGYLY
                     MTDIMFSPEYSSTIGEIFAQIFIDKGPSHVLTVETKGIPIALMTARALNVPMVVARRD
                     SRVTEGPSVGISYVSGSGQKIHNMSLAKRALPENSRVLIIDDFMKGGGTAQGMTELVG
                     EFKAQVVGVAMVIATAVPKEKLVSDFTTLLVLEGIDAQKGEINISINEQLM"
     gene            16988..17266
                     /gene="spoVG"
                     /locus_tag="PK768_00095"
     CDS             16988..17266
                     /gene="spoVG"
                     /locus_tag="PK768_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HQA59405.1"
                     /translation="MNITEVRIRKVEDEGKMKAIVSVTFDNEFVVHDIRIIEGKNGLF
                     IAMPSRRTASGKFKDIVHPINSETRAKIQDVVLSHYNDIDLGENEGTS"
     gene            17349..18746
                     /gene="glmU"
                     /locus_tag="PK768_00100"
     CDS             17349..18746
                     /gene="glmU"
                     /locus_tag="PK768_00100"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HQA59406.1"
                     /translation="MESFTAVILAAGEGTRMKSDTPKVLHKICGKPMLAYVIEAARGA
                     GAQQVVVIVGKAADRVKEAFDREDVEFVLQEEQKGTGHALMQAEEAVKGSSRIVVLCG
                     DMPLVTAESIREMVRLQQEQKAFATVMTAKVDDPTGYGRIIRKGDNVVDIREHKDASP
                     EERKINEINAGIYCFDTASVFSALAKVKDDNIQGEYYLTDVVKILYQEGKEVIAYRLE
                     DPTELQGINDRRQLAQAQQIMQQRIIEGLMVEGVTFINPDTCTVDHGVRIGRDTVIYP
                     GVILEGSTKIGRGCTIIGPCRIKDSELGDSCEIIMSQIDECMLGEGVKVGPYSNLRPG
                     CILANKVKVGDFVELKNAKVDEGTKIPHLSYVGDAVLGKNINIGAGVIFVNYDGYNKH
                     QTLVEDNAFVGCNSNLVAPVTVRAGSFVAAGSTITKEVPEDALAIARARQENKLEWAK
                     KRRNKFKGGTNGNGK"
     gene            18730..19677
                     /locus_tag="PK768_00105"
     CDS             18730..19677
                     /locus_tag="PK768_00105"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQA59407.1"
                     /translation="MAMESRLEVFTGNANPKLAQEIVRHLGIPLGDSWVGTFSDGEIQ
                     VRIKETVRGADVFVVQPFSYPINEHIMELLIMIDAFSRASAGRITAVIPYYGYARQDR
                     KARSRDPITAKMLADILTAVGADRILTMDLHAGQIQGFFNIPVDHLKAMPIIADYFKA
                     KELGEIVVISPDVGGVTRARELADRLAAPLAIIDKRRPEPNVAEVMNVIGKVVGKTAI
                     IVDDMIDTAGTITLGAQALLDRGAKAIYACCTHPVLSGPAIERLKAAPIQEVVVTNTI
                     PLRESQKLDKIKVLSVATLFAEAIRRIHENRSLSSLFDV"
     gene            19789..20412
                     /locus_tag="PK768_00110"
     CDS             19789..20412
                     /locus_tag="PK768_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HQA59408.1"
                     /translation="MEQPVLTATTRIAKKGENKRLKRENKIPAVVYGKEFENKLIAVD
                     EKEFRKLLKTHGEVALIDLQVEGDSFPVLIKEVQRNTLKNAIDHVDFFKVSMDEEVEY
                     HAPIVLVGDAEGVKMGGILQHQKREITLKALPADMLESVEVDISEMNIGDTIAVADLK
                     VDGKNTVLDDADEVVVTIVPPRLAEEVEAPEEGEEPELVEEDKDEEE"
     gene            20454..21023
                     /gene="pth"
                     /locus_tag="PK768_00115"
     CDS             20454..21023
                     /gene="pth"
                     /locus_tag="PK768_00115"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQA59409.1"
                     /translation="MYLIAGLGNPGREYEDTRHNVGFTTLDLIAHSLDLKINKVKFKG
                     LLGEGSHEGRKLLLLKPQTYMNLSGESVKDALNFYKIPPEKLIVIYDDMDLPLGRIRI
                     RPEGSAGGHRGMESIIYQLASDKFPRVRIGIGRPEGERDAAGHVLGRFYGEEIDKIRA
                     AIRAAAEAALMIVAEGVDRAMNKYNGFEA"
     gene            21236..21598
                     /locus_tag="PK768_00120"
     CDS             21236..21598
                     /locus_tag="PK768_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59410.1"
                     /translation="MNRFNIKGSKHAGSMTGSMLRRFFWGLGAAVLTYVVVPKMRRMA
                     KPAIEKGVSSVKELAERGRQTIEEYRAQKEDAVDMIKDASMEQVEVQKDAISGKINAL
                     QETIDRLKNEIMELRNKL"
     gene            complement(21601..21732)
                     /locus_tag="PK768_00125"
     CDS             complement(21601..21732)
                     /locus_tag="PK768_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59411.1"
                     /translation="MPSRATTRQKNLYPDMAKGTLFPKNLALSKKAAWRGRLCPQAA"
     gene            21854..22342
                     /locus_tag="PK768_00130"
     CDS             21854..22342
                     /locus_tag="PK768_00130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HQA59412.1"
                     /translation="MPIVLTRIDDRLVHGQVTVGWTRAYNINTIVVVDDEIATDPIQR
                     SILQMTAPPGIKVTALTVDEFIDRYKKAHYDKARTMLIFTNPSGIVKVVESGVTISSV
                     NAGGMRFSPGKNRISKAISVDEENIQNFRKLLEMNIEVECRMMPSEPKVKIESLLSKG
                     VK"
     gene            22344..>23197
                     /locus_tag="PK768_00135"
     CDS             22344..>23197
                     /locus_tag="PK768_00135"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HQA59413.1"
                     /translation="MLGTALIVAILAGFITIEYIAFGQFGFSNPLVSASLVGLVLGDL
                     QTGITVGITLQLIWMGIVGIGAAVPPDVVTGAILASAFAILTGEGAEIALAIAVPVAI
                     AAQTLDIFVRTLQTGLIHWADRLTERGNYKAIERVHLLGLPITFLRSFIVVFPAIYFG
                     VDSVKAFISTIPQVVIHGLEVAGGMLPALGFGMLLNMIGASFLLPYFFIGFALATFLD
                     VSLIGVAVIGASAAFLHDYFCRRSMDYDEVDTPQAEAEDYQRILTRQDLLTLFRRTFL
                     LQTSWNFER"
BASE COUNT         6672 a         5197 c         6165 g         5163 t
ORIGIN      
        1 gccgattaca gatcctagaa aagctttgaa aggatgagtt aaatgggacc tttggaaaag
       61 gcggagattc tcatcgaagc cttaccatat ataaagaagt tttacggcaa gaccgtcgtc
      121 atcaagtacg gcggccatgc catgactgac cctgagctga aggataaggt gataaccgac
      181 atcgtcctga tgaagtacgt ggggataaac ccggtaatcg tccacggcgg agggccggaa
      241 ataaaccagt ggctgaaaaa gaccgagacc ccttctgaat ttgtaaacgg aatgcggatc
      301 accgatgaaa agatcatgga gatagtgcag atggtcttgg tggggaaggt caacaaggaa
      361 atcgtcagcc tggtggaaaa aaacggggga aaggccatgg gcctcagcgg catcgacggc
      421 cagatgatta aggcccggaa gaagagggcg tatcttaacg gctcggaagt ggacctgggt
      481 tttgtagggg agatagagga gatcgatccc cagctgatac acacggcctt aaggaacgat
      541 ttcatcccgg tcatcgctcc cgtaggtacc ggcaacggcc aggtttacaa tataaatgcg
      601 gactatgtgg cgggcagcat cgccgcgagc ttaaaagctg ataagctggt gctgcttacc
      661 gatgtggaag gagtgttgga caaggaaaag aagctcatat cctctttaaa cttccgggaa
      721 gcccgcaaca tgatagaagc aggcgttatt gaaggcggca tgatacctaa aatcgagtgc
      781 tgcatggaag tcctgtcaag gggagtagag cgggtccaca ttatagatgg gcgcctacct
      841 cacgccctgc tcctggaaat cttcactgac cagggtgtag gcaccatggt agtaaaggag
      901 tgactttgat ggaaaattta attgccttag gcgagaagta cgtcatgaac acctataagc
      961 gcttgcccca tgtcctggtc aagggctccg gctcctatgt ctgggacagc gagggcagaa
     1021 agttcctgga ctttgtcagc ggtatagccg tcaattccct tggccactgt ccccaggtgg
     1081 tgactgaggc catcaaggag caggcggaaa aactcatcca ctgctcgaac ctatactgga
     1141 tcgagccaca gattaaactg gcaaaattgt tagtagaaaa atcctgctgt gacaaggcct
     1201 ttttctgcaa cagcggcacg gaagccaatg aggcggcaat taaacttgcc cggaaatggg
     1261 gccagggccg ctatgaaatc atcagcatgg aaaaatcctt tcacggccgg accctagggt
     1321 cccttgccgc aactggccag gccaagtacc aggaggcctt taagcccctt ccggcaggct
     1381 ttaagtacgt gccatttaat gatttggagg ccttggaggc ggccattact cccgagacct
     1441 gtgccgttat gctggagtgc atccaggggg aaggaggagt aaatgtcccc gaaccaggat
     1501 acttggaggg cgtccaggaa atctgccgga aaaacaatct gctcttaatc ctggatgaag
     1561 tccaaacggg tctgggcagg accggcaagg cttttgcata tcagcacttt aatttaagtc
     1621 ccgatatcat taccctggcc aaggccttag gagggggagt ccccatcggg gccatgctgg
     1681 ccaaggacga agtggccaag gccttccagc cgggagacca tgctgctacc tttggcggaa
     1741 atcccctggc aacggcggcg gctctggcag cctgctcggt ccttttggat gaagaattca
     1801 tagtggaagt tgcaaaaaag ggcgagtacc tgaaggggga acttgaaaag ctaaaggcgg
     1861 aatttcccca gatcatcaag gtaaagggat taggcctcat ggtgggctgc gagctcaata
     1921 cggatgccct gcccatcgtt gaatcttgca tgcaaaaagg agtacttatc aatgccgtgg
     1981 gaggcaacat tttgcgcttt gtgccagctc taaccgtgac gaaagcagaa atagaccagc
     2041 tagtaagcac attaaaagag gccctttcgg aaagcggcaa ataaaaaaac ggggaggaaa
     2101 agcatggaca caggacttaa aggcagagat tttctcagct taaaagactt tagtgcagag
     2161 gagataaagt tcttattgga ccttgcggca aagcttaaag ccgaacacaa gcagggggtg
     2221 tgccatgata tcttaaaggg caagaccctg gccatgatct ttcaaaaggc ctctaccagg
     2281 acccgggtgt cctttgaagt ggggatgttt cagcttggtg gaatggccca cttcctgagc
     2341 acccaggatc tccagatagg ccggggcgag cccatcagcg atacagccag ggtgctatcc
     2401 aggtacgtgg acgggatcct gattaggacc tatgcccagg ccgaagtgga ggaactggcc
     2461 gagtacgccg atgtgcccgt catcaacggc ttaacggacc tgtaccatcc cacccaggct
     2521 ctggcagaca tgcttacgat tttagagtac aaaaagaagc tctcaggcat caagctggcc
     2581 tacataggcg atggcaacaa catggtccat tccctgatga tcggtggaac taagcttggc
     2641 ctggaagtca gggtggccac tcccaaggga tacgagccat tgccggaggt agtcgaaatg
     2701 agcgaggcct tcgccaggga aagcggcggc aagctcattt tgggaaatga cccagaagag
     2761 ataagccatg gagcggatgt gctgtacacc gatgtctggg ccagcatggg ccaggagcag
     2821 gaagcaaaag agcggaaaaa gcgctttaaa ggctttcaaa tcaacaaaaa gatactggaa
     2881 cttgccgaca gtgaggcaat cgtcatgcac tgcctgcctg cccaccgggg agaggaaata
     2941 acggatgaag tgatggaagg gccccaatcg gtggtgtttg acgaggcgga aaacaggctc
     3001 cacgcccaca aggcgatcct ggcggcactc ctttaaaagc attcatattc tgttaaatta
     3061 ttccaaattc cttgtaattt ttccctctct gtgctaaaat tacgttgtac ctaaaattta
     3121 atggacggtg cagaataaat gatagcgtat aaactaggag attctttgta tctgaatatt
     3181 accaaccggt gcaccaacga ctgcgaattt tgcatacggc agcaggcacc gggggtagga
     3241 ggatacaacc tctggctcga ggaagagccg acgaccaagg aaatcattga agcaatagga
     3301 gatcccacag gctacaagga agtggtgttt tgcggatacg gcgagcccct gatgcggctt
     3361 caggtggtta tcgatgtggc aaagcacctc aaaaagacat atcccaatgt tcccgtcagg
     3421 gtcaacacca acggccaggc aaacatgatt tacggcgagg atgtgacccc tcagctggag
     3481 gggaacatag atgtgatttc cataagcctc aacgcggaaa atgcagaaaa gtacagtgag
     3541 atatgccatc cggagtacgg cgaagatgcc tttttcagca ttttagaatt tacccgaaaa
     3601 tgtaaaagcc acattccacg ggttatcgtg acggttgtcg atattcccgg catagatatt
     3661 gcaaaatgca aaagacttgc cgaggagctg ggcgtggaat tcaggcttcg aagtcttgat
     3721 aaaagatagg tttttatcct atcttttttt aacatatgga caacgccatt atgagtaagt
     3781 ttacaaacaa catccttatt ggcaagcttg gattaacgtt ttcgagccaa ctgcaaaaat
     3841 ttttagggag ttatgcgtca gaaaatcaaa gctcattatt aatatttctt aaaaaatata
     3901 aaactaattg tggatagaaa agattaacaa gttaaggcct tatctttgca cgggcttaac
     3961 ttgccaagga ggaaagcgtc attaatatca ggcaaataat gcgaaggtac aaccttaggc
     4021 ccaacaagcg gctgggccag catttcctgg tggacagccg gcccctttac gccatgctgc
     4081 aggcggcaga acttttgccc gaagatgggg tcctggaaat cggcccaggc ctcggagtgc
     4141 tcaccaggga gctttgctca agggccggga aggtggttgc cgtggaaaag gacaggcaac
     4201 tcatacctat actggaggat ttgaccaaag atttcaaaaa tatttgtata ttagatgcag
     4261 atgtgctaaa attagatatg gaggagctta gagaagaaca ctttggcggc agctttaaag
     4321 ttgtggcaaa tcttccttac tacatcacca gtcccattat tatgaagatc atagaaaatc
     4381 gccacttaat tccccttgcg gtcttaatgg tccaaaagga ggtagcccag aggcttgtcg
     4441 cctcgccggg cagcaaggat tacggcattc tcaccatcgc ggttaatatg tacgccgaag
     4501 tgaagctggt ttgcaatgta ggcaagagct ctttcctgcc gccgcccaag gtggaatcgg
     4561 cggtggtgaa gatcttgctt aggcaaaagc cgagggttga agtcgacgaa gaacttttct
     4621 ttaaagtggt ggaagcggct tttggcgagc ggcgaaagac cttaaaaaac tccctcagca
     4681 ggcggctcga tattccgggc attgaccctg aagtaatagc caaggccttg gaggtgaccg
     4741 gcatcgatcc gatgcgccgg ggcgaaaccc tttccatcga ggaatatgcc caactgctgg
     4801 aagcgctgaa aaagtacgca ggcgagactt aactttctgt gaaagcccat atttggccca
     4861 ggactgtttc aagcagtgat gctaaataca aggcagtaca tacacttttg tcattttgga
     4921 gatgggatga tcaccatgat ttttgtaaat ccaacaaggg gcaggatgtc cattaaagaa
     4981 gtagtcagag acatcatagc ttttatggag gaagtacctt cggcttcgta caagttaata
     5041 atcggcaccg actctcaatc cagggacaac tcttgcttcg ttactgctgt aattgtccat
     5101 cgggtgggaa agggagcccg ctactattac tacaggaaat acgtgtccag cgtaaagaac
     5161 ctgaggcaca agatatttac ggaaacatcc atgtccctgg acgccgtgac ccgattgaaa
     5221 gaagagctgg ccaagacccg ctataaggac atggacgtgg aaattcacgt ggatatcggt
     5281 caaaacggcg acaccaagga gctcatccgg gaagtggtgg gctgggtcat gggcgtggga
     5341 tacaaggtta agattaagcc gaatgcctgc ggcgccacca aggtggccga cagacacacc
     5401 aagtaaggca aagggcattt cctttagtta ggaaatgccc tttttgactt agcttccatc
     5461 aagcctttga cagaaagctt tccagtatgt caaagcgcct gaagggattg tatccctggt
     5521 attccttata caggtaacag cggcttcggg gcctcccact gaaataattg atggcctcga
     5581 gggtgtcgcc gataatggta tcggcaattc tcttggcgtg aaagtagtcc cttatctttg
     5641 agccgaggta tttttgcggc agttttgagg gcacctgtac ggaaaggccc tgctttctgg
     5701 caaaggccag gaccatgggc ggcaccgcaa agtacctaaa atcgatgcct tccattaatt
     5761 tgcgggaaac cgcatggggg ccgtgggagg gggtggccac gccgatttcg tcgtaaaggt
     5821 ccagcttgag attgagtaaa ctccgaaaga ggagtatctg ttcggtaagc tcgctcttgt
     5881 actccaagct ggggtagcaa tcggtcatgg ccaggtccgc tccctgacag gtcaccgaat
     5941 ggaccagttc atcaacgagt gtccgaatat tgccgatcaa atccccgtcg acgaagacca
     6001 cgcagcccgc cccctccctg taagcgtaat aggccccgat ggccctggga atatctatgc
     6061 caagctcggg agtaaagtac aggatttcca agttttccag tctcagcccc tttacttctt
     6121 tcatggtatc gtccgaggaa ccgtttacca ctacgacgat tctatctaca ggagtttgtt
     6181 tcagcaaact gagaaccctg ccgattcttc cctgctcatt ttttgcagga acaacggcca
     6241 tgttcatttt tctcacctcg tggtgtctat ctgctcacat cccccctgct agcatcatat
     6301 tatgtaataa accgaataat ggtgaaatat aatgaaatgg gggatacgtg tggctaaagt
     6361 caaaataggg gatgtggtag cccgtgtatc atacaataaa gatgtttttt tcaaagtggt
     6421 tgacatcatg gaggataagg gctgctgcac tttaaaaggt ctcaacgtga gagtgcttgc
     6481 cgatgctccc atcgatgact tgattttacc cagctcgacc gaagtgaggg aatacaagag
     6541 ggattacatc aaaaagagca acgagtccat ggagcggatc ttctaccggc gggaactgga
     6601 aaaacagcgg cgcatgacgc ggaacaacgc tgaggggagc aacggttttt ttgatttacc
     6661 ggggatggtt ctacacatcg acggtgatga agagtatctg gacctgtgtc tcaccaccta
     6721 taggcaactg gacattgaag cctatggcga atgcatagcc gaaaaaaatc agcccaagag
     6781 aatcaacagc ctcttggtca aatacaatcc cgatatcctg gtcattaccg gccatgacgg
     6841 tctgcttaaa gaaaagaggg actttaacag ggtggacaat taccggaact ccaagtactt
     6901 tattgaagcg gtgcgaaatg ccaggcgctt tgagccatcc ctggatgatc tggtgatatt
     6961 tgcgggagca tgccagtccc actacgaggg gctgctcaga gcgggagcaa actttgccag
     7021 ctctccccac cgggtcttca tacacgcact ggacccggtg ttcataatag agaaggtggc
     7081 atacacccct attaataaaa tcatatctgc ccgggacgtg atagacgcca ccataaccgg
     7141 tatcaaaggt ataggaggcg ttgagacccg aggcaagttt agggagggtc ttccccgatc
     7201 gccctacgag taatcggcat tggtggtgaa tctaatggac tttgacaatg aggaactcac
     7261 ggtaaaactc tatcacaccg accagcagga ggcgcggata gtcggcataa gggaatatgt
     7321 gataggggcg gtggctgcca gcatgcctcc cgaattcccc gtggaagccc taaaagccca
     7381 ggccatatgc tgcaggaccc tggccgtaaa gcgcatgggt attttcggag gaggcggctg
     7441 caagcgccat cccggctttg acctttgcaa cgatcccgtg cactgccagg gctttatggg
     7501 cattgaggaa aggcagaaag cctggggcag ccgctacgaa gaatttacgg agaagattgc
     7561 aactgccgtg gaggacactg cggaaataat actgacctat aacaacaatc ccatagaggc
     7621 ggtataccat gaagcctgcg ggggctgcac ggaggattca gaaaatgtct ggggaaataa
     7681 ggtcagttac ctgagacggg tggaatgcat atactgcaag gattcgccct actggaatac
     7741 taccaaaaat ttttcgataa gggagttaaa gcgtaggctg gccatcaact ttgatgacgg
     7801 cttttccgat aagagggaga ttccggggct gctggaaaac attcattcca cagcctccgg
     7861 ccgcataaag aaactaaaga taggggacat ggtctttcag gctgaagata taaagaacct
     7921 cctgggcctt tcctccacca agttttcatg gaaggtttca gcactctcct tccaggtgat
     7981 gggccagggc aacggcctgg gcctttgcca gtacggagcc aggggcatgg cccttttggg
     8041 ctttcccgtg gatgagatat taaagtttta cttcacgggc gtggagctaa agggcttgga
     8101 aaggcccact tatgccaagc ccctcatagg caagaccata gtcatcgacc cgggccatgg
     8161 cggcgatagc ggcaattccg gccccatggg gctgtcggag ggctatgtaa atctcgaaat
     8221 agcaagggtc gcggcggagg gccttgtgca gtgcggggcc aaggtgatac tgacccgcga
     8281 caaaaatgaa tttgtgagcc tttcggaaag ggtccgcatt tccaatgaaa acaagcccga
     8341 cataacttta agcattcagc agaacttttt tcccgaggaa gactcgggtg gaacggaaag
     8401 cttttattat cccggcgatg tggaaggcaa gaaattagcc tgcatcatac accgggaact
     8461 ggtgaccatt ttacagctga gggatttggg ggcaaagccg gcagacctat ttgtgctccg
     8521 ggagaccaac aatccttccg tcatggtgca tatcgcagca atcagcaatc cggaggagga
     8581 acgtctacta tccgaacccc agttcaggca gaaagctgct gtggcgataa tcaatggaat
     8641 taaagcctat tatcacgaat aaacaagata aatttcgcct tttactatta atcatttttt
     8701 tttgtaccta gacttattga caaaaatgac taatgttgat aaactattat aatatttgac
     8761 attcggacaa aagggtggta taattatact gtattttgca tttttgtgag gtgatgttat
     8821 ggcttcggca gatgctatca agaagattcg cagagacgtc cagtcacacc ttgggaagat
     8881 ggttaaactc agagcaaacc gcggccgtaa aaagaccttt gaaaaagaag gcgttttaga
     8941 acaggtttac cccaacatat ttgtcgtgaa agtaaccgag tcgccggatt acgtaaggcg
     9001 gatttcctat agctactccg atatattgac cgataccgtt gaactaacgg ttttgcaaaa
     9061 tgataaacca cagtcaaaat gcggctcagc ataatcccga ttctttcggg attatttttt
     9121 tgtcccttat acacaattgc cccctttcgc caatatatat agatatataa gcgtcgaaag
     9181 ggggtttttt tgtggaaagg atttcccgaa ttttgcggca gcatgagaag aaactcttat
     9241 gcctggaaaa tgtggtgggc gtgggcttag gatataaaat aataaaggac aaaatgacca
     9301 acaagcccgc catcatggtc cttgtgagag aaaagattgc cgatgaaaaa ctgctgggcc
     9361 gaaattgcgt gcccaaggtt ttgagcaata ccccgaccga tgtggtggaa gtaggtgatg
     9421 tgaaactatt gaatgcccga atcgagaggg caaggcccgc caagcccggc atgagcatag
     9481 gccattacaa gatcacggca ggcacctttg gggccctggt gaaagatgta aagacgggcc
     9541 gactcctgat cctgtccaac aatcacgtcc tggccaatgc caccgacggc ggtgacggga
     9601 aagccgtgat cggtgacgcg atactccagc cgggaggcca cgacgggggg acatcgtcgg
     9661 atgtaatcgc ccatttggaa agatttgttc cgatcgcaaa aaacgctgca gaaagctcct
     9721 gcccgatagc ccaaggccta gaaaaggcca tcaacagcct tttaaaagtg ataaagcctg
     9781 actacgaaat caattttatc aaaaaaagca cggccaggaa cctggtagat gcggcggtag
     9841 caaggccagt gagggatgag tatgtgagcg gggaaataat gggcctgggt aaaatttcgg
     9901 gcatccagga gcccaagatc ggcatgaccg tccaaaagag cggccgcacc acgggaatca
     9961 cctccagcca aatacaggcc ttaaatgtgg tgataaaggt catgctaggg ccgaccgagg
    10021 aagctacctt ttacgaccag atcctcacgg gtcccatggc ccagcccggc gacagcggtt
    10081 ccattgtagt cgatgagaac ctgagggcgg taggcctttt atttgccggc tccgaccagg
    10141 caaccctcat caatcccata gtaaacgtac taaagaccct ccaagtagaa cttcccggtg
    10201 ccatgcacta ataagttaat atttggtgcc tggcacctaa cttattgaaa actccaaaca
    10261 aggcatacga ttaagtgcca aaagttgagc aatatggtaa aaacaagaga ttttcgtggt
    10321 taaattaacc ataataggct tttattgaac aattttaata atatcttcat catatgtaat
    10381 ttgttgcatc tcagaagggc ccctgaccga ttataaagat atttgctgtt tgtcgaaaaa
    10441 agcagaaaaa ctatataatg tttacaacat ataatctgca gcaaatctta aaaggaggca
    10501 atacaaaatg aagcagattt acaaaaaaat atgtgtggct cttcttttaa cttttatgct
    10561 gacactaccg ggaattgcca ctgcagcaac gcaaattaat gtgaaagtaa ccctaaacgg
    10621 cagaacccaa gtacttgagc ttccaattga tttagaatcc atcagcaaaa actacagcac
    10681 gacttacagg tttgtctaca cttacaaaga tggcaaatgg atactaacgt ctaaggaaga
    10741 tgctggccag ggccaggcaa agcccagcct tcctaccgtg ccttcaaagc ctcagcccca
    10801 aacgccggct ccctctccat cacagccaaa gcccgagaca ccggctccca gcacgccgga
    10861 tgttaaaggc ctgactgccg atgagagcaa gatgctggaa ttggtgaacc aggagaggca
    10921 aaaactgggc ctaaagcccc tcacaatcga tatgcggcta gttgacattt cccgcaaaaa
    10981 gagcaaggac atgatagaca acaattattt cggccataca tctcctacat acggaactcc
    11041 ctttgatgca ttgaaaaaca acggtgtttc ctataggtat gcaggtgaga acctggcggg
    11101 ggcacccacc gtcgagcggg cccacagcgg cctcatgaac agcccgggcc atcgcgccaa
    11161 tatactaaac cccaacttca cccacatcgg cataggcgtc gtggaaggcg gcccctacgg
    11221 caagatgttc acccagacct tcatcggggt aaattaaagc aggtcataac tggcataaat
    11281 agtgcaaaaa gagctgcctc gcaatcagcg agcggctctt tttttgtaat gatgagaaat
    11341 tattcacatg ttctgttgat tttttgtgga taacttgttg acaatgtgga taaacctagt
    11401 cggtacaata attttaaccc gttatgtata taattttttc tccccctcac catttaaatc
    11461 cgcctacata taatatattg agtgtattct ccattttgcc agaagggaag ggggaaaatg
    11521 aaagtcctca tatttattgt cgtcctagcc attgcctcat gggtcttcta cttgttcata
    11581 cggaaggcct ttgatatgcc tttggaccag accaagggca actccggcca ggttaaaatc
    11641 aaaccagtag ggtcggagga agtcaaaagg gatgaaagct taaagcaagg tacttatgaa
    11701 gaagaagtat cgcagcagcc ccagcctaaa gctgaagatg gctgctgcaa agcagccacc
    11761 gcttgccggc agcgagacga ggaagaaatg acacaaatcc aagaatagtt ttttagctgt
    11821 aacatatttg tcaagcttca catatattat aggagtaagg tggtgaattt tagggaaagg
    11881 aggattttaa tgggtgtcta tttgacaaag gaactggtaa gggtagataa ggtagtcggc
    11941 gaagacaaga cccaggccat agtagaagga accatcacgc tacctgatgg gaagccggac
    12001 atagagcggg tcatttccat tgacgcaacc ctggacacag aaagcttgga gaccgacatc
    12061 ctggattcta aaataggcaa ggtgatagtt gaaggcgaag tggatgtcaa tgccatttac
    12121 gtggcggatg ttccggaagg ccagccacag cagccggttc attttgtaga aggtaaaatt
    12181 gactttagct tttttgtaaa aattcccggt gtcaaaaaag atatggacgt cagggttagg
    12241 gccaagatcg aacacatcca gcacagcttc gaccccaaca ggccgaggga aatcaaggtt
    12301 agacttgtcg tcatgttctt cgtgaaggtc accaggcggg ttgaaatcga gatcgtcatc
    12361 gatgctacgg ggccaaaaga tctccaggtg ctcaagaaaa ccctgcgcat cgaggatgtc
    12421 ataggtgaag ccagggccca gaacatagta aaaagcgatg taggtgtgcc ggaggaaaag
    12481 cccagcatcg agcaaatact gaaggtaaag gcggaagtca gggatgtaac cagcaagatt
    12541 attaaaaaca agataatcat cgaaggcgta ctggaagtgg gtatcctcta cgtggccata
    12601 gctccagaag gtcaacccct gcagccggtg cacttcatgg aagccgagat accctttacc
    12661 caatttgtgg agattcacgg cgccgatgag ggtatggcca ggtttatccg ggtggaagtg
    12721 gagcacataa agggccgccg caaggacgat aggaccgtca ccgtagaggc aatactcaag
    12781 atcagggcca aggtcttcga gaccaagatt atccaggtag tcatagatat cttcagcccc
    12841 tccaaggaac tgcacgtaga aaagaaacgc ttaaaactcg accaggtcat cggcgaagat
    12901 gaaaacgaga tcgtcatcaa agacacgctg acggtgcccg atgaaaagcc ggacatcgag
    12961 cagatataca agaccagctg caaggccagg gtgaccgagt ccaggataat cgacggcaag
    13021 gttatcgtgg aaggggtcct gacattagag accctctacg tagccgatgt gccggaggga
    13081 gagccgcagc agcccctgca cttcatggag cacgagatag actttactac ctttgtggaa
    13141 atacccggtg ccgaagaaga catgatgctg gactttgatg tgatagtgga gcactgctct
    13201 tcatcggcaa tacccaatga tccgaggcgc ttcgaggtca gggcggtgct aggcctcttt
    13261 gccaaggtaa ctcaaatggt tgagcttgac atagttgtac acgtggaaga agagtatgta
    13321 gaagaggaag aagaagaaaa agaggaaaag aaggagaaaa aggagaagaa ggacgataag
    13381 ccggccatga ccatctatat cgttcagagg ggggacactc tctggaatat agccaagagg
    13441 tacaatgtca ccgtggaagc catagttaga gctaacaaca tagccaatcc cgattccata
    13501 atgccgggcc aacaactggt gatacctaga atgtaatcct tatatcccgc aagctgcggg
    13561 atttttgttt tgcctggctc tggaaataat aatgctgaga ggcggatatt ttggcaaaaa
    13621 gggccaaaaa agtacgttat ttttgcagac cggcaggatt ttaagttttt ttgtgaaata
    13681 tataattaga gtataatata tgagaggaga acagcttgaa aaagataacc atagaagcaa
    13741 aggcaaaaat aaacctaacc ctggacgtgc tctacaaaag gcccgacggc tatcacgaag
    13801 tggaaagcat aatgcagacc gtttctttaa aggactggat tttcctaaaa ctcattccgg
    13861 gcccggctat tgagcttatt tcaaactgcc aagagcttcc ccgggatgag aggaacctgg
    13921 cctacaaggc cgccaggctg atgatggagg aataccagct ggatgccggc atggcaattc
    13981 acatagacaa gaacataccg ataggggcgg gcctggcagg aggaagtgcc gatgcggcag
    14041 cggtgatcct gggcatcaac gagatgtttc agctaaacag gccccgggaa gagctgatgg
    14101 ccctgggcaa aaaaataggt gccgatgtgc ccttttgcat agccggcaag actgccctgg
    14161 ccaggggcat cggcgaaaag attctgcccc tcaagcccct gtccaatttt ggcctggtgc
    14221 tggcaaaacc cccttactat gtttcaactc aggaagtata cagtagactg gatgtaaaaa
    14281 ccatacaaaa gcggcccgat acccctgcca tgatggaaca tatcgagcag cgggatatag
    14341 gggaaatagc ctctggcctg tgcaatgtct tggaagaagt gaccttgaaa ctttacccgg
    14401 agctggaact gataaaacag catctcatct cccatggagc cttgggcagc cttatgtcgg
    14461 gcagcggacc caccgtcttc ggcctatttg aaagtgctcg ggatgccgaa agagctgcgg
    14521 agaagatagg gcttgatgga aatgaaattt ttgtgtgcaa aatgcagtag aatttttagc
    14581 aggaggtgat tttgtggaaa acaacagatt tccctcttta aagcttgaca actacaaacc
    14641 cctcagagac atagtctttg aagccataaa ggaagcaatt ttgaaaggcc gcctaaaacc
    14701 cggcgaaagg ctcatggaag ttcaactggc cgaagaaatg ggagtttcca ggaccccggt
    14761 gcgggaggcc ataaggaaac tggaacttga gggcttggtg gtgatggttc cccggaaagg
    14821 tgcttacgta tcgggcctca ccatgaaaga cgctgcggag gtcttcgaaa ttcgcagcag
    14881 tcttgaaggc ctggctgcag cactggcagc agagcgcata accgatgacg aaatcaaaat
    14941 gctggataaa atcctatcgg agattacaaa ggccagcgaa aagggcgatg ccgaaacggt
    15001 catcaaaaag gacgttgaat ttcaccagat tctcttcaat gcttccagga acgaaaggct
    15061 ggcccaaatt gtaaacaacc tgaaggagca gatagacagg tttcgcatcc agtccttcag
    15121 caatcccctc agggtgaaaa acgtggtatc tgaacataag gctatcatag atgctataaa
    15181 gcagggtgat tcggaaagtg cagaaaagct tgcaaaagcc cacatatatg aagtagaata
    15241 taatgtaatg gagatgctta gaaaacgaat gtcttttgag gaggaacttt catgaaggcc
    15301 cttatactgg caggagccga aaaagaaggt cccctggcac aatacggccc taacaaagcc
    15361 ctgataaaga tccacggcaa ggaaatgata cgatacatca tcgatgcctt caagggcctt
    15421 gacttcatag acagcatcgc tgttgtcggg ccgaaggaga gcctggagcc catcaaaggg
    15481 caggtggatg tcatcgtaga cagcgcagct tccctggccg acaacgtcct gtgcggtgtc
    15541 aatattttct cggacgatga aatggtcttg attgccactt cggacattcc catgattacc
    15601 ccggaagctg ttagggattt tgtggaaaag tccctttcga tggatgcgga tttttattat
    15661 cccatcgttt caagagaaga aaacgaaaaa aagtatcccg gaataaagcg cacatatgtt
    15721 aaaataaaag atgggacatt taccggaggc aatatcgtca tggtcagggc cggcaaggtc
    15781 aaggagtgca taaaaaatgc ggaagccttc atgacctacc ggaaaaagcc atggatgctg
    15841 gccaggatcc tgggccttaa atttattctt aagttcctga tgggtacact gaccatagat
    15901 gagctgcagg agcgtgttga ggaacttttt ggcgtaaaag ctcgggcgat cataagttct
    15961 tatcccgaaa tagcaaccga cgtggacaag gacagtgacc ttgagcttgc ggccagagca
    16021 ttaagttagg gggatttttt atgaggcgca gtgagagaat tgccctgatt tcgaacacgc
    16081 tgtcgaggaa cccgaataaa ataatttccc tgaatcactt cagccagtgg ctgggggcgg
    16141 ccaaatcgtc cataagtgaa gatatagata tcataaaaaa agcctacaag aattcagggc
    16201 agggtgatat cgccacgatt cctggagctg caggaggcgt gcgctatcga gccaagaggt
    16261 ctttggatga tatcaatgct tttttacatg acctcagcga ggtgttgcgg gaccaaaagc
    16321 ggatcattcc cggaggctac ctgtacatga cggatatcat gttttccccc gagtattcca
    16381 gcaccatagg agaaattttt gcgcagatct ttatagacaa agggccgagt catgttctga
    16441 ctgtggaaac aaagggcatt cccattgctc ttatgacggc tagggcccta aacgtcccca
    16501 tggtggtagc ccgcagggac agccgggtga cggagggccc ttcggtgggc atcagctacg
    16561 tgtccggttc gggccagaag attcacaaca tgtccctggc caagcgggcc ttgccggaga
    16621 actcccgggt cctgataatt gacgatttca tgaagggcgg cgggacggcc cagggcatga
    16681 ccgaactcgt gggcgaattt aaagcccagg tcgtgggagt cgccatggtg attgccacgg
    16741 cggtgcccaa ggaaaagctg gtgagtgact tcactacact actggtcctg gaaggcattg
    16801 atgcccagaa gggcgaaatc aacatcagca tcaacgagca gctcatgtaa aaaatttact
    16861 tcctatagtt tttacggcag gaatttttaa ttttttatag aatttttaac aacaaccata
    16921 tgctaacaca gaccgacata gttcgaagca tcggctcaag ctaaataaat tcccgggagg
    16981 tattctcatg aatatcacgg aggtccgcat acgcaaggta gaagatgaag gcaagatgaa
    17041 ggcgatagta tccgttacct ttgacaatga atttgtggta cacgatatac gcattataga
    17101 ggggaaaaac gggctcttta tagccatgcc cagccgacga actgcaagcg gaaagtttaa
    17161 ggacatcgtg catccaatca actccgagac aagggcaaaa atacaggacg tggtattaag
    17221 tcattacaac gacatagacc taggagaaaa tgaggggaca tcataggatg tcctatattt
    17281 ttcaaagttg ggaatatctg gaacatacat gcatatattc ccgacagatt cgttagagga
    17341 gggctatgat ggagagtttt acagctgtaa ttttagcggc tggcgaaggg acccgcatga
    17401 aatcggatac tcccaaggtc ctgcataaaa tttgcggaaa gcccatgctg gcatatgtga
    17461 tagaagccgc gaggggggca ggggcccaac aagtcgtagt aatagttggc aaggctgccg
    17521 acagggtaaa agaggccttt gaccgtgaag acgtagagtt cgtactgcag gaggagcaaa
    17581 aggggactgg ccacgccctc atgcaggcag aagaagccgt caagggaagt tcccgcatcg
    17641 tggtgctctg cggagacatg cccctggtta ccgcggaaag catccgggaa atggtccggc
    17701 ttcagcaaga gcaaaaggct tttgccacgg taatgacggc aaaggtggat gaccccacgg
    17761 gttacggcag gataataagg aagggcgaca atgtcgtcga cattcgggag cacaaagacg
    17821 ccagtcccga agaaagaaag attaatgaga taaacgcagg tatttactgc tttgatacag
    17881 catcggtatt ttcagccctt gccaaggtga aagacgacaa catccagggt gaatactacc
    17941 ttacggatgt agttaaaata ctataccagg aaggtaagga agtcatagct taccggctcg
    18001 aagacccgac ggaacttcag ggcataaatg accgcaggca gctggcccag gcgcagcaaa
    18061 tcatgcagca gaggatcata gaaggcctga tggtagaggg ggtaactttt ataaacccgg
    18121 atacctgcac cgtggaccac ggcgtccgaa taggaaggga taccgtaatt tatccgggcg
    18181 tcatcctgga aggaagtacc aagataggcc ggggttgcac catcataggt ccctgccgta
    18241 ttaaggactc ggagctggga gactcctgcg agataataat gagccagata gatgaatgca
    18301 tgctgggaga aggggtaaag gtgggcccct attcaaacct gagacccggc tgcatccttg
    18361 ccaataaagt aaaagtagga gattttgtag agctgaagaa cgccaaagtt gatgaaggca
    18421 ccaaaatacc ccacctatcc tacgtgggag atgcggtttt gggcaagaat ataaacatcg
    18481 gcgccggagt aatttttgtt aattacgatg gatacaataa acaccagacc ctggtggaag
    18541 acaacgcgtt cgtgggctgc aattccaacc tggtagcacc ggtcacggta agggcgggtt
    18601 cgtttgtggc agcaggctcc accatcacta aggaagtgcc ggaggatgct ctggccatag
    18661 cccgagctag acaggaaaac aagcttgaat gggctaaaaa gagaaggaac aaatttaaag
    18721 gaggaacaaa tggcaatgga aagtaggctg gaggttttta ccggcaacgc taaccccaaa
    18781 ctggcccaag aaatcgtcag gcacctgggg ataccgctgg gggattcttg ggtcggcacc
    18841 tttagcgatg gtgaaatcca ggtgaggata aaggaaacgg tgaggggcgc tgatgtcttt
    18901 gtggtgcagc cattttctta tcccatcaat gaacatataa tggaattgct cataatgatt
    18961 gacgccttca gcagggcatc ggccggccgc ataacggctg tgatacccta ttacggctat
    19021 gcccgccagg accgcaaggc ccgctcccgg gatcccatca cggccaaaat gctggcagac
    19081 atactcactg ccgtgggagc ggaccgcata cttaccatgg acctgcatgc gggccagatt
    19141 cagggcttct tcaacatccc ggtagaccac ctgaaggcga tgcctatcat agccgattac
    19201 tttaaggcaa aggaactggg agagatagtc gtcatatccc ctgacgtggg cggagtgacc
    19261 agggcccgtg aactggcgga taggctggcg gcacccctag ccatcataga taagcgccgg
    19321 cctgaaccca acgtggcgga agtaatgaat gtcataggaa aggtggtggg caagaccgcc
    19381 atcatagtag atgacatgat agacacggca ggcacgataa ccctcggggc ccaggccctt
    19441 ttagaccgcg gcgccaaggc catatacgcc tgctgcactc acccggtcct ctcgggtccc
    19501 gcaattgaac gacttaaagc ggcgcccatt caagaagtgg tagtgacaaa caccatcccc
    19561 ctcagggaaa gccagaaact agacaagata aaagtccttt cagtggcgac ccttttcgcc
    19621 gaagcaatcc ggagaatcca tgagaacagg tcccttagca gcttgtttga tgtataaact
    19681 tgccttctgc atgaaggact agtataatat ataagagtac ccgggatggc catgactaaa
    19741 attcagttag tgatggaatg aagcaaaaca gtgaaggagg agtacatcat ggaacaaccg
    19801 gtattgacag cgactacccg cattgccaaa aaaggcgaga acaagaggct aaaaagggaa
    19861 aataaaatac ctgctgtggt ttatggcaag gaatttgaaa acaaactcat tgccgttgat
    19921 gaaaaggagt ttagaaaact tttaaaaacc cacggagaag ttgcccttat agaccttcaa
    19981 gttgaaggag acagctttcc ggtgctgata aaggaagttc agcgcaacac cttgaaaaac
    20041 gcaatcgacc atgttgactt tttcaaagtt tctatggacg aagaagttga ataccatgca
    20101 cccatcgtcc tggttggaga tgcggaaggc gtcaagatgg gcggcatact gcagcaccag
    20161 aagagggaaa ttaccttaaa ggcactgccc gccgacatgc tcgagagcgt cgaggtggac
    20221 atctcggaaa tgaacatcgg tgacacaatt gcggtggccg atttgaaggt ggacggcaaa
    20281 aataccgtcc tggatgatgc agatgaagtc gtggtcacga tcgtgccacc tagacttgct
    20341 gaagaagtag aagctcccga agagggagaa gaacctgaac tggtagaaga ggacaaagac
    20401 gaggaagaat aagggcgaag ggcccttatt ttttccatta atgggggatt ggcgtgtact
    20461 tgattgcagg cttaggaaac ccgggaaggg aatatgaaga cactcgtcac aacgtgggct
    20521 ttacgaccct tgacctgata gcccacagtt tggaccttaa aataaacaag gtaaagttta
    20581 aggggttact gggagaaggc agccatgaag ggcgcaagct gctgctttta aagccccaga
    20641 cctatatgaa cctgtccggc gaaagcgtaa aagatgccct aaacttttat aaaatccccc
    20701 cggaaaagct gatagtgata tacgatgaca tggaccttcc cctgggccgc atcaggatcc
    20761 ggccagaagg aagcgccggc ggccaccggg gtatggaatc cattatctac cagctggcct
    20821 ccgacaaatt tccccgggtc cgcataggca taggccgccc cgaaggggaa agggatgcgg
    20881 cgggccacgt cctgggaaga ttttacgggg aggaaataga taaaataaga gctgccatcc
    20941 gggcagcggc cgaggcggcc cttatgatag tggccgaagg cgtggaccgg gccatgaaca
    21001 aatacaacgg cttcgaagct taaagagtgc atagcactct tttatttttt atcgtttgct
    21061 gcagttttca attgcagatt tcagatacat aataaagtca aaaagggaga aaaatataaa
    21121 caggtcaaat taccccttct attcaaccaa gcggggacgg tccccatgaa tgaatatttg
    21181 gggacagtcc ccaaatattc attatttact ctacttaatt aggagcggtg aaaggatgaa
    21241 tagatttaac attaaaggca gcaaacacgc gggaagcatg accggttcca tgctgaggcg
    21301 tttcttctgg ggcttgggtg cggcagtcct tacttacgta gtagtgccca aaatgcgccg
    21361 catggctaaa ccggctattg aaaaaggtgt tagcagcgtc aaagagctgg cggaacgggg
    21421 aaggcagacc attgaagaat acagggcaca aaaggaagac gcagtggaca tgatcaagga
    21481 tgcttccatg gaacaagtgg aagtccaaaa agatgcaata tccggcaaaa ttaatgccct
    21541 gcaggagacc atcgacagac tcaaaaatga aataatggag cttagaaaca aactgtaaca
    21601 ctaagccgcc tggggacata gcctacctct ccaggcggct tttttgctaa gggcaaggtt
    21661 cttggggaaa agagttccct ttgccatgtc gggatataga tttttctgcc gagtagttgc
    21721 tctactcggc attttttttg ctcattatca ataacatata ctaagcaaat aaatctaaat
    21781 gacaggcatt gggggtgatg gctcatgtga cgaaggctgt cgaaaacata ggaaagagaa
    21841 aggggttgag cttttgccta ttgttctcac acgcattgat gatcgattgg ttcatgggca
    21901 ggtaacggta ggttggacgc gggcttacaa tatcaatact atagttgtcg tggacgacga
    21961 aatagcgaca gacccaatac aaaggtcaat cttacaaatg actgctccgc cgggcatcaa
    22021 ggtgacggct ctgaccgttg atgaattcat cgaccggtat aaaaaagctc attatgacaa
    22081 agccaggaca atgcttatat ttactaatcc ttcaggcatc gtaaaagtag tcgaaagcgg
    22141 ggtgacaatt tcaagcgtga atgccggtgg aatgcggttt agcccgggca aaaacagaat
    22201 tagcaaggcc atatctgttg acgaagagaa catacaaaat ttccgaaagc tgcttgaaat
    22261 gaatattgaa gttgagtgta gaatgatgcc ctccgaaccg aaggtgaaaa ttgaaagctt
    22321 attatcaaag ggggtaaagt aaaatgttag gcactgcttt aatagtagct atcttggcag
    22381 gttttattac tatagaatac atagcattcg gtcaatttgg atttagcaac cctttagtat
    22441 ctgcctcact tgtcggatta gtgcttggtg acttgcagac cggcattact gtaggtataa
    22501 cactacagtt gatatggatg ggtatcgtgg gaataggcgc agcggttcct cctgatgtag
    22561 taacaggtgc gattttggca tcggcctttg caattttaac aggtgaaggt gctgaaattg
    22621 cactggccat agcggtacct gttgccatcg ccgcccagac cctggacatt tttgttagaa
    22681 cgctccaaac agggttaatt cactgggcag acaggttaac cgagcgcgga aattacaaag
    22741 cgatagaacg ggttcacttg ctcggccttc cgattacgtt tttgcgctca tttattgttg
    22801 tttttccggc aatctatttc ggagttgatt cggtgaaagc cttcatttcg acaattccgc
    22861 aagtggtaat tcatgggctg gaagttgccg gcggtatgct gcctgctctg ggttttggaa
    22921 tgttgcttaa catgataggg gcttcatttt tacttccgta ttttttcatc gggtttgctt
    22981 tggccacttt tttagatgtc agcctgattg gagtggcggt aatcggcgca tctgcagcct
    23041 tcttacatga ttatttttgc cgtaggtcaa tggattatga cgaggttgat actcctcagg
    23101 cggaggcgga agactatcaa cggattctta cacgtcaaga tttactaact ctctttagga
    23161 gaaccttctt attgcagact tcgtggaatt tcgagcg
//
LOCUS       DAONUO010000002        21086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_1813_length_21086_cov_4.943464, whole genome shotgun sequence.
ACCESSION   DAONUO010000002 DAONUO010000000
VERSION     DAONUO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 21086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21086
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1813_length_21086_cov_4.943464"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..964
                     /locus_tag="PK768_00140"
     CDS             2..964
                     /locus_tag="PK768_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQA59414.1"
                     /translation="MKILYLMKMLLEKTDEENTMTINDMIAELERYGITAERKSIYDD
                     IEALRHYGIDIATRKTKTTDYFVASRVFELPELKLLVDAVQCSKFVTHKKSNDLIKKI
                     ESLASSHQAALLHRQVYVTNRVKAINENIYYNVDRLHVAIAENKQVSFKYFDYDIKKE
                     KRYRKNGELYFVSPYALSWDDENYYLITFSEKYNDFIHYRVDRMTNIEIIDQPRAILP
                     DNEQFNIVDYSKKVFNMFGGEEETVKLKFDNSLVNAVIDRFGKDVSLDKVDDNSFSIR
                     INVAISSTFFAWLAQFGNKVKILSPESVIEKYKNSIQEILEQYK"
     gene            971..1498
                     /locus_tag="PK768_00145"
     CDS             971..1498
                     /locus_tag="PK768_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59415.1"
                     /translation="MSEDGSLKSSIRPNGYILPDNLRGRSIHAKVIPTVCNLENMLQK
                     LIQINGDFAQLKQWEKRSYKAYQIEEIKNRIMTSPQYAWKDIVREHILSKRPSDFGAS
                     VIDIYLVAYVAETIGAGKDEFFKYIKNAGISDNGNSAQAIWQVGKGDGVYLEILHDNG
                     QVRDWNFMTKWVEGK"
     gene            1514..2236
                     /locus_tag="PK768_00150"
     CDS             1514..2236
                     /locus_tag="PK768_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HQA59416.1"
                     /translation="MKNNRYSCVGIDGCKGKWVVVCITENAFEVEKLKTINDICSRYL
                     DSDSYIIDIPIGLAENKSQLRPDLIVKKELGKKGSSIFEVPCRQAVYAEDKKVARERN
                     IAILGKSLSEQTLGIAKAIKQVDEFLQSKPQWKNRLLESHPEFCFSKLNNNQPILEHK
                     TSAEGQQKRLGILRKYYPKADQVIEKFLADVPYRKKIDDVVDALCLAVMGRVILEKGL
                     KTIPEKPMMDDKGILMQMVYAE"
     gene            2327..3634
                     /locus_tag="PK768_00155"
     CDS             2327..3634
                     /locus_tag="PK768_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59417.1"
                     /translation="MKKLSNAADTKEISLTIYNGGFGAVKEIRKVNLSGSETEVIFAD
                     VAQQIETDSLLVEGLNIIEFNYDYDLVDRDKLLKKYIDKEVYLKDRKTGEKKSCRLLS
                     VEGAGRCVLEDNDTKEIYIDTQAEIVLPSLPSGLIVKPALVWKIGKSTSEDVKVSYLS
                     KGFIWNANYVIEILEKTLNIAGWAEIENQSGTTFENVKIKLIAGDVNRIQDDDRYVME
                     DRMYNCESSAAPQAEEKAFFDYHMYTLLNPTTLKDNQTKQINILNGLNIPYKKYYKLN
                     MNEERANVIVEFTNKKECGLGYAMPKGKIKLYKADEADNSLEFIGEDSIGHTPKDEDI
                     KLTIGNAFDITFDFTEVDRKKLNGFEHYKFECIIRNHKEEAAEVHFEHYVWGIWEMVY
                     ATHEYSKKTSGLIEFSVNVPADSEIKVEFEYKIDRRTEVVVRK"
     gene            3637..4689
                     /locus_tag="PK768_00160"
     CDS             3637..4689
                     /locus_tag="PK768_00160"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HQA59418.1"
                     /translation="MKNDICHFRGCLIGGAIGDALGYTVEFMNSEEIRRKFGNAGITE
                     LICDQRTGKALISDDTQMTLFTAEGILWAHFRGSHKGICSFPSCVFYSYQRWLYTQTG
                     YLASEDYNWILNNEEVDYKSELLNVKNLFVRRAPGNTCLSALSNSTKQNFGTIERHIN
                     NSKGCGGVMRVAPVGLYFYNSPQKAFRIAADCAAITHGHPSGYLSAGVLAFIIAEIIT
                     GKEIKQAALNAIDILKTYEGHEECLEILHKAIDLVEKEIEPQEAIRRLGEGWVGEEAL
                     AIALYCSLKYENDFEKALCLAVNHRGDSDSTGAICGNILGAYLGVNAIPPKWVKNVEL
                     SELITSLSNKLLAVSV"
     gene            4908..8069
                     /locus_tag="PK768_00165"
     CDS             4908..8069
                     /locus_tag="PK768_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQA59419.1"
                     /translation="MKKLYYDLFNSNRRAELVKECIRYINAGLKVFYILPSREAMFDV
                     RRHFTTELGGIFGWHVFGFDDLEQLILGKFLNNAKIIGDLEERMLLKHVLKELPEDTV
                     FDKVKDKPGFLDLLIHTIRKLKRLCISPDEFRSRTAELKGNLLRKCQGFQCIYSGYER
                     IKTEKGLMDIDDISIEAVEACTSTKLFENTAVIVVDGFINIDPVNIKLLKNIQQEYPS
                     ISVYANIPFRNRNNEQFLMDEVIRDFKQMGFEMIENDGSSGISVNPCLQSIAHHIYSG
                     EAYIKVDAENLKIINSPCMDHEIRIAAGKAKELIQNQGVKLSDIAVITADTENYKPKV
                     IEIFEEYGVPISVKQQSKLPSIPMIKDTMALWRLGLQSEYSMAFVSIVTSKYMLPIEI
                     LSDQGFETDKLFKLAEIILKKDGQGNYFEAFLGEYIETTENELSKEAMRSYINAVCAF
                     EKALHGQPLDAIGAFKEFLETIQIERNISALYRERLLNEKLWLRDMEAIMELSKFFDW
                     LHHGYKEYGFDQTDIEPEGLIKDIFDMIAGVETGDITRDTSGVRFIPPDLARGQTYDT
                     VFVLGVNEGIFPAGGNKAQLFDVNEIRELSDIGIRLSNSEWELEREKLRFNACMASAR
                     NRLYLSYRTADEDGSVMIASPFIDEIVSILDKESKGKVIEKPVSMRDRVSFRGETWSI
                     SEAVKAINTHSRNNTAFDKSVITQEVRGRLEYPVHAAAIEFSREVGSEFNAYDGKLEH
                     PVLAQQDVSYGFSASQLNSYSRCPFTYFAQRVLDLTVEDDNIKEMLDTGTFYHTVLKA
                     YHLGNQNPCVPDIERLDTIFNEMSGQLTFDSIPVPLKKYIMEELLMVLRNFIIHDAEN
                     MSRYYQTTGCTLKPVMLEEPFKMTIGKENSILKGVADRVDLELDSKGAYTGRFIIYDY
                     KKGSIRSIKECIEGDDFQLPLYYSAFNNIIKERFHIGQPECLALLYYSIEKLDWNGII
                     RKDIKKALFEGRKGTKSTPDKANMEVVLSWAGNEATSIINNIRKGYFMLPKECPASSM
                     FGCLYSGICRYDRTRLAQKEGVL"
     gene            8069..11404
                     /locus_tag="PK768_00170"
     CDS             8069..11404
                     /locus_tag="PK768_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQA59420.1"
                     /translation="MLEDLCKIYEVNEEQARALDIHLNTALRAGAGSGKTRVLTKRFV
                     RCLLENPTLTLDNIAAITFTRKAATEMKDRVRRELSDRISKIDNAVEKNRLSNIKMQI
                     TNSNIDTIHGFCGKILRDHFAFLGLDPDFNIMEEVDKSVLLSEIADSVIQSFIENEKN
                     EVIVRSLAENFSTGFFTGKLKQGILSAFGAMREKGMDVVSFCQEFKTGGIENADPITV
                     LESTALILINNLDNEYRAYKEKKNLLDFNDLEILSERLLRNNEIRTSYFSRFTTILVD
                     EFQDVNPLQKKILDHLIWEQGKIPEGRLFIVGDHKQSIYGFRGSDYRVFEEACSEVLG
                     CGKVEFLNNCYRSTKNIIGAVNSIFSQLLSPYEKLQYPGQGDSDGRKVELITWEKGLS
                     KENKPKTRWDLAKNLLLSEDMKEELKCALEAEYEDIAVAGKKDYQGDVIAGVIQKLTA
                     EDFLYRDIAILLRSRTSLSEIENSLTRNGIPYCVLGGIGFWDRQEIGDILSLYKLVFF
                     PDDRLALFTVLRSPIFGFSDDMLLALSIFMRKQKIKNLDELLTAFGEMVAEDDKWVVK
                     RAADVLASLLPMDGILNSLELMNKIIKATAYDEVLTALPKGEKKLRNIEKLIRIIEEF
                     DAKGIYSARELLPYLDVLKESSGMDGEAFLDNEDSDAVKILTIHASKGLEFRAVLIPD
                     MDRPLDSQAKRSKPLFFLDDQKGLIAMGLDESLKLNENANPEYAKLYAEKLRRELEDS
                     RRLFYVAATRAKEYLGFIGEKQEVGSEEEIDAQNSFMKQLIWAMNKVGTVEEITSVDA
                     MSLVPNQQKQSVYPPPFIAEIKKLTGINSIENAMNQRLEPCERHEEGNISISSWMKYR
                     DCPRRFYLENLAGLHDKRDAQYTEGLPAAYERMNAADFGSLLHAFLEEVDILDLNKSG
                     FKDNTSEVAAAIDIELPQADRELFEKEVAGFIKIEEKRRKENKGTLVTSLKEFGFRVP
                     LAEGINLTGFIDRVDIFEREGQYIASIIDYKSNRLNNSHAAEEKASNYKEQLLSYAWA
                     LNQIPFYNGKKVIVEEALLYFLNNGEVVSIELRKEDIEGVVEALINSAPWLLGNKTLS
                     EFQCCKSEKCSWCNCKKYCDDLN"
     gene            11506..12084
                     /locus_tag="PK768_00175"
     CDS             11506..12084
                     /locus_tag="PK768_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQA59421.1"
                     /translation="MDKTDINVRYEKLKEIVKERKEQFDELIAFMENETAWLTSPAST
                     KYHLCEEGGLLKHSINVAETMLKIKNAIAPDISDESCVIVALLHDLGKVGMPGRPQYL
                     INEPSEKQMKYGYKPDYPYRFNKELTYLSVPVRSIYLSMKYIDLTEEEVQAIVYHDGQ
                     YVEDNRSCATHEAPLTLLLQYADSWSGFVIEK"
     gene            12179..12586
                     /locus_tag="PK768_00180"
     CDS             12179..12586
                     /locus_tag="PK768_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59422.1"
                     /translation="MDKIRAYESTDKQMIPLSIAPKFEAKFNKMSTHLQEVFTELIIK
                     CSKFCSKCFPTNTPDFRLQKSFNFCLINFVNSKVRIHIRTDGLKLESEHLTIRPLPNH
                     HYNGWEWVEVAIDKECEVDEAVMLIEKAFRHSD"
     gene            12675..13337
                     /locus_tag="PK768_00185"
     CDS             12675..13337
                     /locus_tag="PK768_00185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQA59423.1"
                     /translation="MYHVFVDFEMTCWRKYDRVIGEKIQEIIEIGAVKLDENFTLIDT
                     FSVYVKPEFSQLLSKTCTKLTGIRNDVIKDAPSLAEAVVMLEDWLGTEDIKFYSWGKD
                     DKIQLERECTSKGLYNKMPTKYLKWRDFQRIFMKVFGFTRRLGLKAAMEMTGFDFEGQ
                     QHRAVDDAMNGARLLMLIKNKDRYKKQRELISEVYNCQKPFTSTVGELIAEKLSAFSY
                     AS"
     gene            13368..14564
                     /locus_tag="PK768_00190"
     CDS             13368..14564
                     /locus_tag="PK768_00190"
                     /inference="COORDINATES: protein motif:HMM:NF039739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6361 family protein"
                     /protein_id="HQA59424.1"
                     /translation="MNIGWIDFSKEQRNKVMSVINLLSEPEAVDELGVGIIRDAFSDI
                     FFPGTSTIQTRAKYFLITPYLLAELEREKGMTPDKMINRLHEQELDFIDILKQSGENG
                     IIGETAGRKLKRKPSDIYWNGIRTFGIFTGGKMSLYDYARITCLLKNKKHNTKVLGSI
                     GLKDDEKDADDIDAVTGELTGGFWKLPDFSADWRDGLTIKLTKQEAEFLRNQIMSSVP
                     DSILYFVLEKNYTDFLQFESFDDIEGMVNLFPHNLKEDYIMAKEFSDFVFGIHLRYNM
                     LLSQGKNDDVITEWKLWSLDMGKCVNIDMQKILFDRLRIKNGKLIKFLFDCKDAMLKN
                     DIEKLDELVIMRERRLKGDKRAKLYNVNEFEYKGWVGIGKLQYRFRNAKNLLRDIFEG
                     IGDVNA"
     gene            14659..16347
                     /locus_tag="PK768_00195"
     CDS             14659..16347
                     /locus_tag="PK768_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HQA59425.1"
                     /translation="MDALIGISIALGLSESIDSGLKDNPIYLLEALNKTADKVLVFCE
                     GGQIKVPANAHALHILLEKMVFEIVLKNKKSFHPKFWLVKYENADGEALYRCLVLSRN
                     LTFDRSWDVAVCLEGKVKIDARLKAGYEKSRPLSDFLQSLLKLAKKNSISPGKKSNLL
                     SLAEEILHVKFEIKDNQFSDYCFYPVGIDGYEIENTGLFNTYHELFAISPFLSDNTIK
                     SFNELALTNPNKNTIITRKSELAKLKPSSVTNFDIYVMKDLIVDGEEAFSDGDNDTTE
                     EKQKQDIHAKVYLKTKYSDSELFLGSLNASYNACYGNVEFLLKLCGKRRYLNVEQLKK
                     DVFGENDKDNPFEKTEIKAFDELEKTEADKLQKMIKEICRLRSYAKALECDDKYSLEV
                     TFEKLPEISNVYISPLLSKKEQEISDKVVFAELSMLELSEFFVIKAVSGEETVKRVIK
                     IKTDNIPVARESAVVNSVIKDKNGFIQYITFLLGDDFLLSMLENNRLASNGFVFGNGE
                     PIPALYEKMLKAAAHSPQKFDEIERLMELISDSSIIPEGFKELYRVFQKAVNKK"
     gene            16344..19628
                     /locus_tag="PK768_00200"
     CDS             16344..19628
                     /locus_tag="PK768_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQA59426.1"
                     /translation="MKIDLKITEKQIMNGLKDFQRATVERVFSLFKNGQNRVLVADEV
                     GLGKTLVAKGIIAKTARYHQEEGDTLFKVVYICSNQNIANQNINKLKISRDITVDGVT
                     DTRLSMQHLKIFEQENNQSIKDKFVQLIPLTPSTSFSMTSGCGSVEERALMFAVLKRM
                     ARFEAYIKELEVILVDLATSSWRWASPWYEKRVAACNDVSDGQYLKIITRTINDYFAE
                     KHIDAELILLCQKVRANGGNRIGGTYDILYKLRMMFAQISVDLLKPDLVIMDEFQRFK
                     FLISADVSTETGILADRFLKGNDIKILLLSATPYKLYSTLEEISETQSDDHYSEFFQV
                     MNFLFKSEKRQQEFKEIWNNFSVKLREINSDYFSIIEVKKSAETAMYQGVCRTERISA
                     VKTGDFIDDKSIKIPLRVTEKDILSYIEAEKLLNEIGIGYSVPVDYVKSAPYILSFMK
                     QYQLKKHIEKFFHKNPDDIKLANKKHLWIDDRVIRNYGELDSTNARLERLKEAAFENN
                     AELLLWIPPSKPYYEPQGVFKNAGNFSKILVFSAWEMVPRMIASLISYEAERKTVGKL
                     AALNPDKQNTSYFAPNNKRFPVARLRFNVVNGNPQAMSLFCLLYPSKFLSELYNPIEC
                     LNEGKCLKDIEIQIKAKVDKLLSKLRKYQSDSNRVDERWYYLAPLLIDYPDYVNAWFS
                     SGVELIQEITDDEEEKGQKGFKLHLEKLKEYYENILSVKLGKMPDDLTEVLANIVLAS
                     PAVCAFRANGYNSIYATQFAKAMINQLNKPEATAVIELCYSPKIEKPIQRKGLLGTFK
                     EMLNSNKFDEAHWRNVLSYCKDGNFQALLDEYVHILVDSNGINDAENKHELIHALMLE
                     AVKTHSASYTIDTFNTFKNNVISKKEKGIALRSHYAVGFYKGEGENSKLINRKESIRN
                     SFNSPFRPFVLATTSIGQEGLDFHYYCRRIMHWNLPSNPIDLEQREGRINRFKCLAIR
                     QNIALKYGNNVIFKNDVWNDLFERAKDDYKTGYSELIPFWCLPEDQEIKIERIVPVYP
                     ISKDIGTYERLIKILSLYRLTLGQARQEELLEYLFKNCDDRDKLKELFINLSPFYKEN
                     MAIKGV"
     gene            19881..20204
                     /locus_tag="PK768_00205"
     CDS             19881..20204
                     /locus_tag="PK768_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59427.1"
                     /translation="MLDKAIRIAAKAHEGQMDKAGQPYILHPLRVMFMRRNETERICA
                     VLHDVIEDSDITIEYLRKEGFSEEVLSALDALTKRENENNDDFIGRVIKNKTACKVKR
                     IQIHH"
     gene            complement(20632..>21086)
                     /locus_tag="PK768_00210"
     CDS             complement(20632..>21086)
                     /locus_tag="PK768_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HQA59428.1"
                     /translation="RDPFELCKATGVEVFYADLGHLKGMYKYLKRNRFAVINEKLDDY
                     IKKLVCAHELGHDLLHRDLARRVCLQEFVLYDMKSRPEYEANLFASEILLPDDAIFSY
                     AHEGYDIEQIARILYTDINLIALKMASMNARGYQFRTVYDPKSDFLKK"
BASE COUNT         7177 a         3211 c         4710 g         5988 t
ORIGIN      
        1 gttgaagatt ctatacttaa tgaaaatgct tcttgaaaaa acggatgaag aaaatacaat
       61 gacaatcaat gatatgatag cagaacttga acgctacggt attactgctg agcgaaaatc
      121 catatatgac gatatagagg ccttacggca ttatggaata gatattgcta ctcggaaaac
      181 aaaaactacg gattattttg tggcaagccg tgtctttgaa ttaccggaat tgaaactatt
      241 ggttgatgct gttcaatgtt cgaaatttgt tacacacaag aaaagcaatg accttataaa
      301 aaagatcgag agcttggcaa gctctcatca agctgcgctg cttcatcgac aagtttatgt
      361 gacgaacagg gttaaggcta ttaatgaaaa catttactat aatgtggaca ggcttcatgt
      421 cgcaatcgca gaaaataagc aggtatcttt taagtatttt gactacgata tcaaaaaaga
      481 aaagagatat cgaaagaatg gagaacttta ttttgtaagc ccttatgctt tgtcttggga
      541 tgatgaaaat tattatctta ttaccttctc ggaaaaatat aatgatttta tccattatcg
      601 agtagacagg atgacgaata ttgaaatcat agatcagcca agagcgatac tgccggacaa
      661 tgagcaattt aacattgttg actattcaaa aaaagtattc aatatgttcg gaggagaaga
      721 agagacggta aaattaaagt tcgacaattc ccttgttaat gcggtcatcg atcgttttgg
      781 aaaggatgtt agtttagaca aagttgatga caatagcttt tcaattagaa taaacgttgc
      841 aattagttct accttttttg catggttagc acagtttgga aataaagtga agatattatc
      901 tccggagagc gttattgaaa aatacaaaaa ctctatccag gagattttag aacaatacaa
      961 ataagggggg gtgtcggaag atggaagtct taaaagttcg ataagaccta atggatacat
     1021 attaccggat aatttaaggg gtaggagtat tcacgcaaaa gtaataccaa cggtatgtaa
     1081 tttagagaat atgcttcaaa agcttataca aatcaacgga gattttgctc agctaaaaca
     1141 gtgggagaaa agaagttata aagcatacca gattgaagag ataaaaaaca ggataatgac
     1201 ctccccgcaa tatgcctgga aggatattgt ccgggaacac attttatcaa aaagaccgtc
     1261 tgattttggt gcaagtgtaa ttgacatata tctggttgca tatgtagccg agaccatcgg
     1321 agctggcaaa gatgagtttt ttaaatacat taagaatgcc ggtatttcag ataatggtaa
     1381 ttctgctcaa gctatatggc aagtaggaaa aggagacgga gtatatcttg aaatacttca
     1441 tgataacggg caggtaaggg attggaattt tatgactaaa tgggttgaag ggaaataaga
     1501 attgatgttg acgatgaaaa ataatcgcta tagttgtgtt ggaatcgatg gctgtaaggg
     1561 taaatgggtt gtagtttgca taactgaaaa tgcatttgag gttgaaaaat tgaaaaccat
     1621 aaatgacata tgtagccgat accttgattc tgatagttac ataatagata taccaatagg
     1681 ccttgctgaa aacaaaagtc agctacgccc agatctgatt gtgaaaaagg agcttggtaa
     1741 gaaaggctca agcatatttg aagttccttg caggcaggct gtatatgcag aagataagaa
     1801 agttgcaagg gagcgaaaca ttgctatttt aggaaaaagc ctctctgaac aaaccttggg
     1861 tatagccaaa gcaataaagc aggttgatga attcttgcaa agcaaacctc agtggaaaaa
     1921 caggcttctt gaaagccatc cggaattttg tttttcaaag cttaataaca atcaaccaat
     1981 tcttgagcat aaaacatctg ctgaaggtca gcaaaaacgt cttggaatac ttagaaaata
     2041 ttatcctaaa gcagatcaag ttatagagaa gtttttagcc gatgttccat acagaaagaa
     2101 aattgacgat gtggtagatg ccttatgtct ggcagtgatg gggagagtaa tccttgaaaa
     2161 aggccttaaa actattccgg aaaagccaat gatggatgat aagggaatat tgatgcagat
     2221 ggtttatgca gagtaaagcg gataagttgt tttatgaatt aaaacgactg tccgttttat
     2281 tgtacatatt atttgataca atttactata aagggaggtg caggtaatga aaaagttatc
     2341 aaatgctgct gatactaaag aaatatcatt gaccatttat aacggcggtt ttggggctgt
     2401 aaaagaaata cgcaaagtaa acctatcagg aagtgaaaca gaagtgattt ttgctgatgt
     2461 tgcacagcag attgaaacgg attctctcct ggtagaaggt ttgaatatta tagagttcaa
     2521 ttatgattat gacttggttg atagggataa gctcctcaaa aagtatattg ataaagaagt
     2581 ctatttaaag gacaggaaaa caggcgagaa gaaaagctgc cgtcttttat cggttgaggg
     2641 tgctggaaga tgtgtattag aggataatga tacaaaggag atatatattg atactcaggc
     2701 ggagattgtt cttccgtccc taccatcagg acttatagta aagcctgctc ttgtatggaa
     2761 aattgggaag tcaacatctg aagatgttaa ggtttcctat cttagtaagg gcttcatttg
     2821 gaatgcaaac tatgttatag agattttgga aaaaacttta aacattgcag gatgggcaga
     2881 aattgaaaat caaagtggta caacatttga aaacgttaaa ataaaactta ttgctggaga
     2941 tgtcaataga attcaagatg atgatagata tgttatggaa gatcgaatgt ataattgtga
     3001 gtcctctgca gcaccacagg cagaggaaaa agctttcttt gattaccaca tgtatacatt
     3061 attaaacccc acaacactga aagataatca gaccaaacaa atcaatattt taaatggtct
     3121 aaatatacct tataaaaagt actacaagct caatatgaat gaagaaaggg caaatgtaat
     3181 tgttgagttt acaaataaga aagaatgtgg cttgggttac gccatgccaa aagggaaaat
     3241 caagctttat aaagctgatg aagcagacaa ttcccttgaa tttattggtg aggacagtat
     3301 agggcatacc cccaaggatg aagatattaa gctcaccatc ggtaatgcgt ttgacatcac
     3361 ctttgatttt acagaagttg ataggaagaa attaaatgga tttgagcatt acaaatttga
     3421 gtgcattatt aggaaccata aggaagaagc cgcagaagtt cattttgaac attatgtctg
     3481 gggtatatgg gaaatggttt acgcaacaca tgagtattca aagaaaacat cagggcttat
     3541 tgaattctct gttaatgtac cggctgacag tgagataaag gtagaatttg aatataaaat
     3601 cgaccggcgt acagaggttg tagtgaggaa atgatgatga agaatgatat ttgtcatttc
     3661 agaggctgtc ttattggtgg ggcaatagga gacgcgttag gctacacagt tgaatttatg
     3721 aatagtgaag aaataagaag gaagtttggc aatgctggaa ttactgagtt aatttgtgat
     3781 cagagaactg gaaaagcttt gatttccgat gatacgcaaa tgacattatt cactgcggag
     3841 ggtattctgt gggctcattt tcgcggcagt cataaaggta tttgcagctt tccatcctgt
     3901 gttttctatt catatcaaag gtggctttac acacagactg gttatttggc aagtgaggac
     3961 tataattgga ttttgaataa tgaagaggta gactataaaa gtgaacttct aaatgttaag
     4021 aacctatttg tgaggagagc gcctgggaac acatgccttt ctgctttatc aaacagtaca
     4081 aagcagaatt ttggcactat tgagagacat attaataaca gcaagggctg tggaggggtt
     4141 atgcgcgttg ccccggttgg attatatttt tataattccc cgcaaaaagc attcagaatt
     4201 gcagctgact gtgccgcaat aacccatggt cacccgagtg gatacctttc agcaggtgtt
     4261 cttgctttta ttattgcaga aataattact ggtaaagaga taaagcaggc tgccttaaat
     4321 gcaattgata ttcttaaaac ttatgaaggg catgaagaat gccttgaaat cttacataaa
     4381 gctattgacc tggtggaaaa ggaaatagaa ccgcaagaag caataagacg cctcggagag
     4441 ggctgggttg gggaagaagc actggcaatt gccctatatt gttcattgaa gtacgagaat
     4501 gattttgaaa aggctctctg ccttgcagtg aatcatagag gtgacagtga tagcactgga
     4561 gccatatgcg gtaatattct tggtgcatat ctcggtgtaa atgctatccc tccaaagtgg
     4621 gttaaaaatg ttgagttatc tgagttgata acaagtttgt ctaataagct gttagcggta
     4681 agtgtctaaa taatatgtta atatggataa atttattcaa taactttgac aagtggcatt
     4741 aatattatga gttatgaatt attacattaa ctgtttgaaa aactggcctt taaaatgtgc
     4801 cggtttttta tctattgcac taaattacag ttacaaaaat acccaccttt catttctaaa
     4861 atctgataaa atatgagaag aagcaccttg gacaggagtg aaatctgatg aaaaaactat
     4921 attatgattt gtttaacagt aatagacgtg cagaactggt taaggaatgt atcagatata
     4981 ttaatgcagg cttgaaagtc ttttatatat tgccatcaag ggaggcaatg tttgatgtta
     5041 gacgccattt tacaacggaa ttaggtggta tcttcggctg gcatgtgttt ggttttgacg
     5101 atcttgaaca gctgatactt ggtaaatttc ttaataatgc taagatcatt ggagacctgg
     5161 aagaacgaat gcttttaaag catgtgctaa aggaacttcc tgaagataca gttttcgata
     5221 aagtaaagga taaacctggt tttttagatc ttttgataca tactataaga aagctcaaac
     5281 ggctgtgtat ttcacctgat gaatttcgtt caagaaccgc agaactaaag gggaatctcc
     5341 taagaaaatg tcagggtttt caatgtattt attctggtta tgaaagaata aaaacagaaa
     5401 aaggtctgat ggatatagat gatatctcaa tagaagctgt ggaagcctgt acaagtacaa
     5461 aattgtttga aaatacagcc gttattgtag ttgatggatt tattaacatt gacccggtga
     5521 atataaaact cctgaaaaat atacagcagg aataccccag tatttcagtt tatgcaaata
     5581 tcccctttag aaacaggaat aatgaacagt tcctgatgga tgaagtcata agggatttta
     5641 agcagatggg ttttgaaatg atagaaaacg atggtagttc cggtatttca gtgaatcctt
     5701 gtctccagtc aatcgctcat cacatttatt caggtgaggc ttatataaaa gtcgatgctg
     5761 aaaatcttaa aataatcaat agtccatgta tggatcatga aataagaatt gctgccggga
     5821 aagcaaaaga acttattcag aatcaaggag taaagttgtc ggatatagcg gttattactg
     5881 cggatacgga aaactacaag ccaaaagtaa tcgaaatatt cgaagagtat ggcgtcccca
     5941 ttagtgttaa gcagcaaagt aaactgcctt ctataccgat gatcaaggat actatggcct
     6001 tgtggaggct tgggctccaa agtgagtatt ctatggcttt tgtttctatt gtaacctcta
     6061 aatacatgct gccaattgaa atactttcag atcagggttt cgaaaccgac aaactgttta
     6121 agcttgctga aattatattg aaaaaggatg gtcaaggcaa ctactttgag gcttttttag
     6181 gtgagtatat tgaaactaca gaaaacgaat tatccaagga agctatgaga agctacatta
     6241 atgcagtttg cgcttttgag aaagctttac atggccagcc attggatgct attggagctt
     6301 ttaaggagtt tctggagacg atacaaattg aaagaaacat ttcagcactt taccgggaaa
     6361 gattactgaa tgaaaaactg tggctgagag atatggaagc tataatggag ctttcaaagt
     6421 ttttcgactg gctccatcat ggatataaag aatacggctt tgatcaaacg gatatagaac
     6481 cggaaggttt aattaaggat atatttgata tgatagccgg cgttgaaaca ggggatatca
     6541 caagggatac aagtggagtg aggtttattc cgccggatct tgccagagga cagacttatg
     6601 ataccgtttt tgttttggga gtcaatgaag gtatattccc ggctggagga aataaggccc
     6661 aactgtttga tgttaatgaa atcagggagc tttctgatat cggaattaga ttgagcaatt
     6721 ccgaatggga gctggagcgt gaaaagctga ggtttaatgc ctgcatggca tctgcccgta
     6781 acaggctcta tctgtcttac aggacagcag atgaggatgg gagtgtgatg atagcatccc
     6841 ctttcataga tgaaatagtt tctattttag ataaagagag taagggaaag gttattgaga
     6901 aaccagtcag tatgagggat agggtaagtt tcaggggtga aacctggagt atcagtgaag
     6961 ctgttaaagc gataaataca cattctcgga ataatactgc ttttgataag agcgttatca
     7021 cccaggaagt acgggggagg ctggaatatc cggtacatgc tgcagctatc gagttttcaa
     7081 gagaggtagg gagtgagttt aatgcctatg atggcaagct tgaacatccg gtacttgcgc
     7141 aacaggacgt aagctatggt ttttctgctt ctcaattgaa tagctattca cggtgcccct
     7201 ttacatattt tgctcaacgg gtgctggacc ttacggttga ggatgacaat ataaaagaaa
     7261 tgcttgatac aggcactttt tatcatactg tgttaaaggc ctaccatttg ggaaatcaaa
     7321 acccatgtgt gcctgatatt gaaagactgg acaccatttt taacgagatg tcaggtcagt
     7381 taacttttga ttctattccg gtcccactta agaaatatat catggaagag cttctgatgg
     7441 ttcttaggaa ttttatcatc catgatgccg aaaatatgtc aaggtattat cagactacag
     7501 gctgtacctt aaagccggta atgcttgaag aaccctttaa aatgactatt ggcaaggaaa
     7561 acagtattct aaaaggagtg gcagacaggg tagaccttga attggattca aaaggtgctt
     7621 atacaggaag atttattatc tatgattata aaaaaggcag catcagaagt attaaagaat
     7681 gcatagaagg tgatgacttc caactcccgc tatactatag tgcttttaac aatattatca
     7741 aagaacggtt tcacatcgga caaccggagt gtctggcact cctttattat agcatcgaaa
     7801 agctggattg gaatggaatc atccgtaagg acatcaaaaa agcattgttt gagggaagaa
     7861 aaggaacaaa aagtacaccg gacaaggcta atatggaagt agtactttca tgggcaggaa
     7921 atgaagctac aagtatcatt aataacataa gaaaaggtta tttcatgctt ccaaaggagt
     7981 gtcctgcatc aagcatgttt ggctgccttt atagtggaat ctgcagatat gaccgtacaa
     8041 gacttgcaca gaaagaaggt gtactgtaat gttagaagac ctttgcaaaa tttatgaagt
     8101 aaatgaggag caggcaagag ctctcgacat tcatttaaat acagccctta gagcaggagc
     8161 aggttcagga aaaaccagag tacttaccaa acggtttgta agatgccttt tggaaaatcc
     8221 aacactgact cttgacaata ttgcagccat tacttttaca agaaaggctg caactgaaat
     8281 gaaggacagg gttcgaaggg agctttctga tagaatttca aagattgata atgccgttga
     8341 aaagaatagg ctttccaata taaagatgca aattacaaat agcaatattg ataccatcca
     8401 tggtttttgt ggaaagattc tgcgggatca ttttgctttt ctggggcttg atccggattt
     8461 taatattatg gaagaggtgg acaagagtgt gcttctttca gaaattgcag atagtgtgat
     8521 acagtccttt attgaaaatg aaaaaaatga agtaatagta aggtccctag cagagaactt
     8581 ttcaaccggg tttttcactg gcaagctcaa acagggaata ctatccgcct ttggcgccat
     8641 gagagaaaag ggaatggatg tggtaagttt ttgccaggaa ttcaaaactg gtggcataga
     8701 aaatgcagat cccattacag tactcgaaag tactgcttta atactcatca acaatttgga
     8761 taatgaatac agggcataca aagagaagaa aaacctgttg gattttaatg atctggagat
     8821 tctatctgaa aggcttctca ggaataatga gatcaggaca agctatttca gccgctttac
     8881 aacaattttg gtggatgagt ttcaggatgt aaacccactc caaaaaaaga tccttgatca
     8941 cctgatatgg gaacaaggaa agataccgga gggaaggcta tttatcgttg gagaccacaa
     9001 acagtctatt tatggcttca gaggctcaga ctacagagtt tttgaagaag cctgtagtga
     9061 agttttaggc tgcggcaagg tggaatttct gaacaattgc tatagaagta cgaaaaatat
     9121 tatcggtgca gtgaacagca tatttagtca gcttttaagc ccgtatgaga agctccaata
     9181 tccggggcaa ggagattcgg acggcaggaa agtcgagctt attacctggg aaaagggttt
     9241 gtcaaaagaa aacaagccta agacaaggtg ggatttggca aagaatctgc tgctctcgga
     9301 agacatgaaa gaagagttaa agtgcgcatt agaggctgaa tacgaagaca ttgcagttgc
     9361 aggcaagaag gattatcagg gggatgttat tgcaggtgtt attcaaaagc taactgcgga
     9421 agatttcctg tacagggata tagcaattct tttaagaagc agaacatctc tttcggaaat
     9481 tgagaactcc ctgaccagga atggtattcc ttactgcgta cttggtggga ttggtttctg
     9541 ggacagacag gagatagggg acattctttc actgtataaa ctggtatttt tcccggatga
     9601 caggcttgcg ctctttactg tactgcgctc gcccatattc ggattttctg acgatatgct
     9661 gctggcactc tccatattta tgagaaaaca gaaaatcaaa aacctggatg agctgctgac
     9721 agcatttggg gaaatggtgg cggaagatga caaatgggtg gtcaagagag ctgcagacgt
     9781 acttgccagt ctcctcccca tggatggaat attaaacagc ctggaactga tgaataaaat
     9841 cattaaagca acagcttatg atgaggtact tacagccttg cccaagggag aaaaaaagct
     9901 ccggaacatt gaaaagttaa tcaggattat tgaagaattt gatgcaaagg gaatatacag
     9961 tgcaagggaa ctacttccat accttgatgt tctaaaagaa tcctcgggta tggatggaga
    10021 ggcttttctt gacaacgaag acagtgacgc agtaaaaata ttaaccatcc atgcttctaa
    10081 agggcttgaa ttcagggcag tattgattcc ggacatggac agaccgctgg attcacaagc
    10141 aaaaagaagc aagcctcttt tcttcctgga tgatcagaag ggactgatag ccatggggct
    10201 tgatgagagc ctcaaactga atgaaaatgc gaaccctgag tatgcgaagc tatatgcaga
    10261 aaaactccgg agagaattgg aggactccag aaggctattt tatgttgctg ctacccgggc
    10321 gaaagaatat ttagggttta tcggagagaa acaggaggta ggatcggaag aggaaattga
    10381 tgcacaaaac agctttatga aacagcttat atgggcaatg aataaggttg gcacagtgga
    10441 agagataact tcagtggatg ctatgagcct tgttcctaac caacaaaagc aaagtgtata
    10501 tcctccacct ttcattgcag aaatcaaaaa gcttacaggc attaactcga ttgaaaatgc
    10561 gatgaatcaa agattagagc cctgtgagag acatgaggaa ggcaatataa gcatatcttc
    10621 ctggatgaaa tatagggatt gccccaggag gttttatctg gaaaatcttg caggacttca
    10681 tgataaaaga gatgcacagt ataccgaagg actgccggct gcctatgaaa gaatgaatgc
    10741 tgctgatttc ggatcgcttc tgcatgcctt tttagaggaa gttgatattc tggacttgaa
    10801 taaatcaggc ttcaaggata atacctctga agtggcagct gctattgata tagaattgcc
    10861 gcaggcagat agggaacttt tcgaaaagga agtagcggga ttcatcaaaa tagaggaaaa
    10921 aagacgaaaa gaaaataaag gaaccttggt tacgtccctt aaagaatttg gtttcagggt
    10981 gcctctcgca gagggaataa acttgacagg gtttatcgat agggtagata tttttgagcg
    11041 ggaaggacaa tatatagcat caattattga ttataaatca aataggctga ataattcaca
    11101 tgctgcggaa gagaaggcaa gcaattataa ggaacagttg ctttcctatg cgtgggcgtt
    11161 aaatcaaata ccattttata atgggaagaa agtaatagta gaggaggctt tgctctattt
    11221 ccttaataat ggtgaagtag taagtataga gttgagaaaa gaagacattg aaggtgttgt
    11281 ggaagctcta ataaactcag ctccgtggct tttggggaac aagacattaa gtgagtttca
    11341 atgctgtaag tctgaaaaat gtagttggtg taactgtaaa aagtactgtg atgatttaaa
    11401 ctgaaattaa atctccttca aaaaacggat aatgcactgt gatgcactat ccgttttatc
    11461 gtacacatca tttgatataa ttaagaaaaa caggagatta agtgtatgga taaaacagat
    11521 ataaatgtaa gatatgaaaa gttaaaagag attgtgaaag agcggaagga gcagtttgat
    11581 gagttgattg cttttatgga gaatgaaacc gcatggttaa catccccggc aagcacgaag
    11641 tatcatcttt gcgaggaagg cgggctttta aagcactcaa ttaatgtagc tgagacaatg
    11701 ttaaagatta aaaacgccat tgctccggat atcagtgatg agagttgcgt tatcgtagct
    11761 ttattgcatg atctgggcaa ggtaggtatg cccggaagac ctcaatacct aattaatgag
    11821 ccctcggaga agcaaatgaa atacggatac aagccggatt atccatatcg tttcaataaa
    11881 gagcttacat acttgagtgt accggttagg agtatttacc tgtctatgaa atatattgac
    11941 cttacagagg aagaagtcca agctattgtt tatcacgatg gacaatatgt tgaggataac
    12001 cgttcctgcg caacacatga agcgccgtta acgttgctat tgcagtatgc cgatagttgg
    12061 agtgggtttg taattgagaa atgaaaagag ttatgataaa tgaaaataat aaagaatttt
    12121 tatggcaagg caaacttcat atttataatt tgttaagaaa attcgtggag gtagcaaaat
    12181 ggataaaatc agggcttatg aaagcacaga taaacagatg atcccgttat caatagcacc
    12241 gaagtttgaa gctaagttta ataaaatgag tacacatctg caggaagtat ttacagagct
    12301 tataataaag tgcagtaagt tttgtagtaa atgctttcca acaaacactc cggattttag
    12361 gcttcagaaa tcttttaact tttgtttaat aaactttgtt aattcaaaag tgaggattca
    12421 tataagaaca gatggtttaa agctcgaatc agaacacctt acaataagac cgttacccaa
    12481 tcatcactat aatggatggg aatgggtaga ggttgcaatt gataaagaat gtgaagttga
    12541 tgaagctgta atgcttattg agaaagcatt cagacactca gattaaccga atttactctt
    12601 acgtttccat atcagatgtg tatcgcgtca gatagtattg gcatataaga tttaagaatt
    12661 gttggaggtg tcttatgtat catgtgtttg tagattttga aatgacctgt tggagaaaat
    12721 atgacagggt tattggtgaa aaaattcagg agattattga aatcggtgca gtaaagttgg
    12781 atgaaaactt tacactgata gatacatttt cagtatatgt aaagcctgaa ttcagccaat
    12841 tgctttcaaa gacatgtact aagcttaccg gaataaggaa cgatgttata aaagatgctc
    12901 catcccttgc tgaagcggta gtaatgttag aggactggct tggaactgag gatataaagt
    12961 tttattcctg gggaaaagat gacaagatcc aattagaaag ggaatgcaca tcaaagggct
    13021 tatataacaa aatgccaaca aaatatttaa aatggcgaga ctttcagcgg atatttatga
    13081 aggtttttgg ctttacaaga agattaggtt taaaagcagc gatggagatg accggatttg
    13141 attttgaggg tcagcagcat agggcagttg acgatgcgat gaatggggca agactgttaa
    13201 tgctgattaa aaataaagac cggtataaaa agcagagaga actgatatca gaggtttaca
    13261 actgtcaaaa gccgtttact tctaccgtcg gagagttaat agcggaaaag ctttctgctt
    13321 ttagttatgc atcgtaaaaa aatatacaga tagtgagttg atgatttgtg aatatcggct
    13381 ggattgattt ttcaaaagaa caaaggaata aggttatgag tgttataaac ttgctttcag
    13441 agcctgaggc agtggatgaa ttaggagtcg gtattattcg agacgctttt tcggatattt
    13501 tcttccctgg tacttctact atccagacga gagcaaaata ctttctgatt accccatatt
    13561 tgcttgctga attagaacgt gaaaaaggga tgactcctga taaaatgatt aaccgcttac
    13621 atgaacagga gcttgacttt attgacatat taaaacaaag cggcgagaac ggtattattg
    13681 gtgaaactgc gggaagaaag ctaaaacgta agccttccga tatatattgg aatggcatta
    13741 gaactttcgg catatttact ggcggcaaaa tgtccttgta tgactatgca cggattacat
    13801 gccttttgaa gaataagaag cacaacacaa aagtattagg aagtattggc cttaaggatg
    13861 acgaaaagga tgcagatgat attgatgcag taacgggaga gcttacaggt ggcttttgga
    13921 agttacctga tttttctgct gactggagag atggacttac tataaaactt acaaagcaag
    13981 aagctgaatt tttaagaaat cagattatgt cttctgtgcc tgattctata ctttactttg
    14041 ttttagaaaa gaactataca gattttttgc agtttgaaag ctttgacgat attgaaggaa
    14101 tggtgaatct gtttccgcat aatttaaaag aagattacat aatggcgaaa gagttttcag
    14161 actttgtttt cggcattcat ttaagatata acatgttatt gtcccaggga aagaacgacg
    14221 atgttattac cgaatggaag ctttggagtt tggatatggg aaaatgtgtt aatattgata
    14281 tgcaaaaaat tctctttgat aggcttcgta ttaaaaacgg aaagcttatc aagtttctat
    14341 ttgattgcaa ggatgcaatg cttaaaaatg atattgaaaa actggacgag cttgtaataa
    14401 tgcgtgaacg aaggctcaaa ggagataagc gagccaagct ttataacgta aatgagtttg
    14461 aatataaggg ttgggtaggc atcgggaaac tgcaataccg tttcagaaat gcaaaaaacc
    14521 tgttaagaga tattttcgaa gggataggtg atgtcaatgc ttaaacctgc atccgacaga
    14581 ttggattaca gcaaaatgat ggcacctccc tttggatatg aaacggtgtt tgcggttggc
    14641 actacttatt cccttgattt ggatgcgctg ataggaataa gtatagcttt aggtctttca
    14701 gaaagcattg atagcggatt gaaggataat cccatctatc tgttagaagc tttgaacaaa
    14761 accgctgata aggtgctggt cttttgtgag ggcggacaga taaaggttcc tgcgaatgca
    14821 catgctctgc atattctctt ggaaaaaatg gtttttgaaa tagtccttaa aaataagaag
    14881 tcatttcatc ccaagttttg gcttgttaag tatgaaaatg ctgacggtga ggcgttgtat
    14941 cgctgtttag ttcttagcag gaatttaact tttgaccgga gttgggacgt tgctgtatgc
    15001 cttgagggta aagttaaaat agatgccaga ttaaaagcgg gatatgaaaa gtctcgtcct
    15061 ttaagtgact ttttgcaatc cttattaaag cttgcaaaga aaaatagtat aagtccgggc
    15121 aagaaaagta accttctaag tttggcagaa gaaattcttc atgtgaagtt tgaaattaaa
    15181 gataaccagt tttcagatta ttgtttttac cctgttggta ttgatggcta tgaaatagaa
    15241 aacacaggac tattcaatac ataccatgag ctttttgcta tctctccttt tttaagtgat
    15301 aacactataa agagttttaa tgagttggct ttaactaatc caaacaaaaa tacgattatt
    15361 acccgaaaat cagagctagc taagctaaag ccaagttcag taactaattt tgatatttat
    15421 gtgatgaagg atttaattgt tgatggagaa gaagctttca gcgatggaga taatgacaca
    15481 acagaagaaa aacagaagca ggatattcac gcaaaggtat atttaaaaac aaagtattca
    15541 gatagcgagc tgtttttagg atcgctaaat gcttcataca atgcttgcta tggtaacgtt
    15601 gaatttttgt taaagctctg tggcaaaaga cgatacttga acgtagagca attaaaaaag
    15661 gatgttttcg gggaaaacga taaggataat cctttcgaaa aaacagaaat aaaggctttt
    15721 gatgaactgg aaaaaacaga agctgataaa cttcaaaaga tgataaaaga aatttgcagg
    15781 ctgcgatctt atgctaaagc tttggaatgt gacgataaat attcacttga agtaactttt
    15841 gaaaagctac cggagataag taatgtatat atatcaccgc tcctttcaaa aaaagaacaa
    15901 gaaatttctg ataaggtggt ttttgcagaa cttagtatgc ttgaactatc cgaatttttt
    15961 gtgatcaagg ctgtaagcgg tgaggaaact gtcaaaagag ttataaaaat aaagaccgat
    16021 aatatcccgg tagcaaggga aagtgccgtt gtcaacagcg ttattaaaga caagaacggt
    16081 tttattcagt acataacttt tttgttgggt gatgattttt tactgtctat gcttgaaaac
    16141 aatagactag caagtaacgg ttttgttttt ggaaacggtg agccaattcc tgcgctgtat
    16201 gaaaaaatgc ttaaagcagc ggcacattcg ccacagaaat ttgatgagat agagcggctt
    16261 atggagctaa tatccgatag tagtattatc cctgaaggct ttaaggagct ttatagggtc
    16321 tttcaaaagg cggtgaacaa gaaatgaaaa tagatttaaa aattactgaa aagcaaataa
    16381 tgaacggact aaaggacttt caacgggcaa ccgtagagag ggttttttcc ctttttaaaa
    16441 acggacaaaa cagagtgctt gtggctgatg aggtagggct tggcaaaacc ttggttgcca
    16501 aaggcattat tgcaaagacc gccaggtatc atcaagaaga gggagatact cttttcaaag
    16561 tggtgtatat ctgctctaac cagaacattg caaatcaaaa tattaataag ctcaaaataa
    16621 gcagggatat tacagttgac ggtgtcactg atacgcgtct ttcaatgcag catttaaaaa
    16681 tatttgaaca ggaaaataac caaagcatta aggataaatt tgtacaactt attcctctta
    16741 ctccatcaac atccttttct atgacaagcg gttgcgggag tgttgaggaa agagccttga
    16801 tgtttgcggt attgaaaaga atggcaagat ttgaagctta tataaaagag cttgaagtga
    16861 tacttgttga tttagctaca tcaagctgga gatgggcgag tccatggtat gagaaaagag
    16921 tggcagcctg taatgatgtt tctgatggcc aatatttaaa aataatcaca aggacaataa
    16981 atgattactt tgccgaaaaa catatcgatg cggaacttat attgctatgc caaaaagtaa
    17041 gggcgaacgg aggcaaccgt attggcggta cgtacgatat tctctataaa ttgcgtatga
    17101 tgtttgccca gattagtgtc gatctgttaa agccggactt ggttattatg gacgaatttc
    17161 agcgctttaa atttttaatt tcagcagatg tttcaaccga aacaggcata ttggccgacc
    17221 gctttttaaa gggtaatgat attaagatac ttcttctctc cgcaactcca tataagcttt
    17281 actctacact tgaagagata agtgaaaccc aaagcgatga tcattattca gaattttttc
    17341 aagtaatgaa tttcttattt aaaagcgaaa agcggcagca agaattcaag gaaatatgga
    17401 acaacttttc tgttaagctt agagaaataa actcagatta tttctcgatt attgaggtta
    17461 aaaaaagtgc tgaaactgca atgtatcaag gcgtttgcag aactgagaga atatctgcgg
    17521 taaagactgg tgatttcatt gatgataaaa gtataaaaat accgcttaga gtaacggaaa
    17581 aagatatctt atcctacatc gaagctgaaa aactattaaa cgaaatcggg ataggctaca
    17641 gcgttcctgt tgattatgtc aaatcggcac catacatact ttcgtttatg aaacagtatc
    17701 agctaaaaaa acatattgaa aaattctttc ataaaaatcc tgacgatata aagcttgcaa
    17761 ataaaaaaca tctgtggatt gatgacagag ttatcaggaa ctacggtgag cttgattcca
    17821 caaacgcaag acttgaaaga ctaaaagaag ctgcctttga aaataacgct gaactgctat
    17881 tgtggatacc gccctcaaaa ccctattatg aacctcaggg cgtctttaaa aatgccggga
    17941 atttctctaa gattttggtt ttttcggctt gggaaatggt cccgaggatg attgcatccc
    18001 taatatccta tgaggctgaa agaaaaacgg taggaaagct tgcagcactt aatcccgata
    18061 agcagaacac aagctatttc gcaccgaata ataagcggtt tcctgttgcc aggctgcgct
    18121 ttaatgtcgt gaatggtaac ccacaggcaa tgagcctctt ttgcttgttg tatccgtcaa
    18181 aatttttatc ggagctttat aatccgattg aatgtttgaa tgagggtaaa tgtcttaaag
    18241 acattgaaat tcaaataaaa gcgaaagttg ataaattgtt gtcaaaatta cgtaaatatc
    18301 aatccgatag taatagagtg gatgaaagat ggtactattt agctccattg cttattgatt
    18361 atcctgatta cgtaaatgca tggttttcca gtggtgttga attaattcaa gaaataacag
    18421 atgatgaaga agaaaaaggg caaaagggat ttaaacttca tttggaaaaa ttaaaggaat
    18481 attatgaaaa tattttatca gtaaagttgg gtaagatgcc tgatgatttg actgaggttc
    18541 ttgctaacat tgttttagct tctccggcag tatgtgcctt tagagcaaat ggatataaca
    18601 gcatctatgc tacgcagttt gcaaaggcca tgattaatca attaaataaa cctgaagcaa
    18661 cggctgttat agagctatgt tatagcccaa aaattgagaa gccaatccaa cggaaaggtc
    18721 tattggggac atttaaagag atgctgaaca gcaataaatt tgatgaagcc cattggagaa
    18781 atgtactaag ctactgtaag gatggtaact tccaagcttt gcttgatgaa tatgtgcata
    18841 tacttgtcga ttctaatggt atcaatgatg cagagaataa gcatgagctt attcatgcgc
    18901 taatgcttga agcggtaaaa acacactctg cgagttatac aattgacact ttcaatacct
    18961 ttaaaaacaa tgttatcagc aaaaaagaaa aaggcattgc actccgttca cattatgccg
    19021 taggctttta caagggggag ggggaaaaca gtaagctgat aaatcgcaag gagagcatta
    19081 gaaactcatt taattcacca ttcagacctt ttgtacttgc tacaacttcc attggtcaag
    19141 aagggcttga ttttcactac tattgcagaa ggataatgca ctggaattta ccttcaaacc
    19201 cgattgattt ggagcagcgg gaggggagaa taaataggtt caaatgtctt gctatcagac
    19261 agaatattgc gcttaaatac ggcaacaacg tcatttttaa aaacgatgtt tggaacgacc
    19321 tgtttgagcg tgcaaaggat gactataaaa ccggttactc ggaattgatt ccgttttggt
    19381 gtttgccgga agatcaggaa ataaaaattg agcgtattgt gcctgtgtat cctattagta
    19441 aggatattgg aacctatgaa aggctgataa aaattctctc gctttaccgc ttgacattag
    19501 ggcaagccag gcaagaagaa cttcttgaat acctattcaa gaattgtgac gatagggata
    19561 agctaaaaga gctatttata aatctcagtc ctttttataa ggagaatatg gcaataaaag
    19621 gagtttgata aaaatgaagt tcggcatgag aaaaccaagt attaggaaaa gtattgcagc
    19681 aagaacgagc ttaaaaagat atatacgtca caacttaggc gtaaaagctc caagagggtg
    19741 gggttggatt acaaatccta aaaaggcctt gtataacaag gtctaccgaa aaaccacgtt
    19801 cagtatattt gatattttta agttttttaa atgaaaatga caataatcca tattgataaa
    19861 gatgaaatgg ggtgtactaa atgcttgata aagctattcg tattgcagct aaagcgcatg
    19921 aaggacagat ggacaaggct gggcagccat atatacttca cccgctgcgg gtcatgttta
    19981 tgcgaaggaa tgaaactgaa agaatttgtg cagtgttaca tgatgttatt gaggattcag
    20041 atattactat tgaatatttg agaaaagaag gtttttcgga agaagttctg agtgcattag
    20101 atgctctgac aaagcgcgag aatgaaaaca atgatgattt cattggcagg gttattaaga
    20161 ataaaacagc ctgtaaagta aagaggattc aaattcatca ttaatgaacg aggtgttgcc
    20221 tcgatcattg aggatagctt atcatatgtt gatggaatac tatgggaaat aactaaagag
    20281 catattgata ttctggatga acgtgaaggt gttaagtata acacttactt taaaaagaat
    20341 gttactgtta cgagtctgga acaagctgga aggcagtaca tagcgcttgt ttatatagca
    20401 tcaaataata gacccgggaa accaagacca ggctatttag aacgagttat tgaaggggca
    20461 cgggaaaacg gaatagataa cgattatatt caaactctga aacagtgctg ggaataaata
    20521 tttttttggt gcaaagagcg aaattaacaa tgaaaaaaca tagtttttat tagtttttat
    20581 atcaaatatc aaaaaaataa ccagagcttt tcgatgaaga aaatgctctg tttatttctt
    20641 taaaaaatcg gatttcggat cataaactgt tctaaattgg tatccccggg cattcataga
    20701 agccattttt agggcgatta aatttatgtc ggtataaagt atccgtgcga tttgttcaat
    20761 atcgtaacct tcatgtgcat aagaaaaaat agcatcatcc ggtagaagaa tctcagatgc
    20821 gaatagatta gcttcatatt caggacggct tttcatatcg tatagcacaa attcttgtaa
    20881 gcataccctc ctggccaaat ccctatgtaa taagtcgtgc cctaattcat gagcacagac
    20941 caactttttt atgtagtcat caagtttttc attgatgact gcaaaacggt tccgtttaag
    21001 atatttgtac attcctttga gatgacctaa atcagcataa aaaacttcaa ctccagtagc
    21061 tttacatagt tcaaaaggat ctctcg
//
LOCUS       DAONUO010000003        16761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_2549_length_16761_cov_3.728421, whole genome shotgun sequence.
ACCESSION   DAONUO010000003 DAONUO010000000
VERSION     DAONUO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2549_length_16761_cov_3.728421"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK768_00215"
     CDS             <1..673
                     /locus_tag="PK768_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HQA59429.1"
                     /translation="KDFATGETLNLNSDQVYVAASTIKLPLVLYIYELAAQGKINLDA
                     KLTYTPEYYAQGTGILQGEPFGGQYTIRELSRLSMEYSDNVAWKMLLDFMGQDKLTAY
                     EKSLGAKATGGDNGLYITTPEDMGIYLERLLNFSEEKPEYGNEVLHYMANSIFSEGIP
                     QDLPDDIVVAHKMGALNDKFHDVGIVFGNRPYIIAIFTDEAWEEVSLQTLADVSRIVY
                     NFQES"
     gene            complement(749..1501)
                     /locus_tag="PK768_00220"
     CDS             complement(749..1501)
                     /locus_tag="PK768_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA59430.1"
                     /translation="MAVRSIVHIDEDKCNGCGLCIPSCAEGAIKIIDGKARLVADKYC
                     DGLGACLGSCPQGAITIIEREAEEFDEEAVEEYLKSSGEDFDEEGFSCPGSSQVFSCP
                     GSRAIDMRQTNKTASTCDTGDVTISIKSQLKQWPVQLMLVPVQAPYFENADLLITADC
                     VAVAYPNYHLGMLKGKAVVMGCPKLDDGNHYVEKLSAILKNNNIKSITIAHMEVPCCF
                     GMVKIVEESLRRSGKNIPIKSMEVSIEGEIIA"
     gene            1642..2973
                     /locus_tag="PK768_00225"
     CDS             1642..2973
                     /locus_tag="PK768_00225"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQA59431.1"
                     /translation="MKRKHIILLFLISILIMWILFSRFSRVTIRQLDDLGTVKITINF
                     YIQVEQEGIEDKIKLSSERPGTEIIKNLIWIDDTTLEILAMEKALPKGFNTELKVGPL
                     KTSIPGLYKLVKVNFSAEISPFLTGMSSIAASTGPIELDFSTPIKIQDLRKGLVADFD
                     YVLRERYLIDENGKVFKDCSSFELVPKEPLVPGKQYEVKFDGLLSNYTDGASKTAFHR
                     IFTVADVPKVVRTQPQDSERDVKLYDQVLVEFDEDMKEVDIEIEKMSGDIKLEGKTAA
                     FKPHTVFVPNTTYRVRVTGKSTFGEAMAPYSFEFTTVDMGDKWWVEINLRPIQKVTVF
                     RGNKIVKSMIASAGLPDEENRTPRGFFTLKDRREYFWTERIQEGGLYWVRITDNYLIH
                     SVPRDKDNEIIEEEFKKLGIPASHGCVRLKDEDAKWFYETVPSGTLVIIHD"
     gene            2993..3583
                     /locus_tag="PK768_00230"
     CDS             2993..3583
                     /locus_tag="PK768_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQA59432.1"
                     /translation="MNFIFSLFILTGIIVAAANGRIDIITTAAMTSAANAVQRAITLI
                     GIISLWLGIARVAEESGLINILSRLIAPLFRWLFPSIPKGHPALGCILMNLSANMLGF
                     GNAATPFGLKAMQELQRLNDKPDTATEAMCTFLAINTSSVTIVPATLIALRASTGSQN
                     PSEIMGAVLFATTCSTMAAILGDIVIRNLYRRRRSY"
     gene            3580..4116
                     /locus_tag="PK768_00235"
     CDS             3580..4116
                     /locus_tag="PK768_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQA59433.1"
                     /translation="MTAEMLGQGFSWFIPGLIFVVFLYGHLRGVKVFEVFVEGAHEGF
                     MMAIKLIPYLIGIYVAIGIFRDSGAVDILVTLLSPILRVIKAPTDALLLLIVRSLSGP
                     AALSMTMEIFDKHGPDSFIGRLASTMLGSSDTTFYIIAVYFGSVGVRKTRYAIPLGLL
                     ADFASFVASVYIVRKMFI"
     gene            complement(4153..5745)
                     /locus_tag="PK768_00240"
     CDS             complement(4153..5745)
                     /locus_tag="PK768_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQA59434.1"
                     /translation="MFIIASLIFLGVLFTTLWQLGLMNPSYIIISLLIIAFYMAIYLI
                     DRKMENDKLTKLVSRLKEFNEGVLGQVFDDIKGGIVGNLSQEIANVTQNTRHLVGELS
                     IASEQLKELCQKFYSETDASAKSSQEIAETISHIAERTEDQVSACSDAVTEIVKLSDL
                     SNLIASETAKVVSGNIEVQKSLQESSQLIEKLVNSIELTSQQNSVTAVEVDALVKEAE
                     KVSGIISSVETIAQQTNLLSLNAAIEAARAGDMGRQFAVVADEIRKLSMNAQIAASEI
                     KDTVQNISDRIVKLSEEIVDGFIKVQVEAQHAKIAKQSLETTSQVIEDTLESMDHINN
                     LTNDEAVAANNIKKTIEDFSSLTQDISAAFQETAAVSEEQAAVMHNINATTASLMKVA
                     DEIYSYVEKVLKDSQYDVSSEIKTKVLNLLKHWVKSKEILSMDKEQHHKVFDKLKKEF
                     PNFTGIITVDHTGRSIANSNPSNVTDFSFRDWFKATKSGQDYTSKMHISALTGLPTIT
                     VATPIFKDNEFIGAMSAGIRLR"
     gene            complement(5867..6895)
                     /gene="ltaE"
                     /locus_tag="PK768_00245"
     CDS             complement(5867..6895)
                     /gene="ltaE"
                     /locus_tag="PK768_00245"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HQA59435.1"
                     /translation="MKYIDLRSDTVTLPTKQMRDAIYNAQVGDDVYGEDPSINRLEEM
                     AAQMLGKEAALFVTSGTQGNQICVMTHTQPGDEVILEEKSHIITYEVGGIGRLAGVQA
                     KLIAGKHGIMDPADIEAAIRDEDIHLPKTSLICLENTHNRAGGTVIPLDILKSTYEIA
                     KRHDIPVHMDGARIFNAATYLNIPVKEIAKHADSVMFCLSKGLCAPVGSVIVGTSDFI
                     ARARKFRKMLGGGMRQAGFLAAAGIVALEKMTSRLAEDHENARFLAQGLSEIPGITID
                     MDTVQTNIVICDISGLKMTAGEFAAKLYEKGIRVNGGSSPIVRFVTHYWIKGEDVEAT
                     IEAVKSLL"
     gene            7058..7453
                     /locus_tag="PK768_00250"
     CDS             7058..7453
                     /locus_tag="PK768_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQA59436.1"
                     /translation="MSGKTFISESVFVDIAKEAMRKVEEVYKQEKKGGLAGFTRMFVD
                     RFTPQISVKKTDPAEFEETAGSVAFEVKISVVYGVKIPEVADKAREKIISDVETLTGY
                     SVEKVDIVVERVIKPEEIQEEKAEQKTED"
     gene            7530..7763
                     /gene="copZ"
                     /locus_tag="PK768_00255"
     CDS             7530..7763
                     /gene="copZ"
                     /locus_tag="PK768_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HQA59437.1"
                     /translation="MAGCCDHCNSDIILNVSGMSCGHCKAAVEKAVGALPGVSKVEAN
                     VEESKVEISYNPQEVSIDDIKQAIEDAGYDVEG"
     gene            7985..8371
                     /locus_tag="PK768_00260"
     CDS             7985..8371
                     /locus_tag="PK768_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HQA59438.1"
                     /translation="MITKGDKILFFSIVLLSLIMFTAFQVYGFANGKTYLLIEVNGKL
                     YQKVLLGRDGPNLTIPVPVTTGENVVEVDADRVRMLYAECPDGDCLRQGWISRPGQMI
                     VCLPNKMVIAIQSDKYISDDIDDVTF"
     gene            8392..8934
                     /locus_tag="PK768_00265"
     CDS             8392..8934
                     /locus_tag="PK768_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HQA59439.1"
                     /translation="MILLKHRKIIFLSLLVSFGIVLHIIENMIPVPFPVPGAKLGLAN
                     IISLIAISFYGLREGLMVSVLRSIIGTLLAGSLSSLIYSLSGAIMSTIIMAFAYKRFG
                     NVFSLVGISFLGGVTHNITQVTVASLALSTLGLYIYLPFLMIIGLFTGLFTGLAAQFV
                     KQNLDTTLIKLGILREDKIK"
     gene            8931..9179
                     /locus_tag="PK768_00270"
     CDS             8931..9179
                     /locus_tag="PK768_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HQA59440.1"
                     /translation="MKRSYRSPLVLVIILLVGLVIGSVLGQVLGSYAPILNQGSSIGL
                     STTNLDLGIINVTFGFNVSLNLAGALGLIIAIIFYQRM"
     gene            9194..9772
                     /locus_tag="PK768_00275"
     CDS             9194..9772
                     /locus_tag="PK768_00275"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQA59441.1"
                     /translation="MERPIILASASPRRQQLLLQLGLKFSVEPSYLDENPDTTMDFGP
                     LAAKMAHSKAMAVASKHDEGLVIGADTIVVLDDRILGKPGTFEEAEEMLRNLSGRWHK
                     VFTGVSLVDAATKRHIKEFEESRVKFKVLSSLEIQNYIKTGEPMDKAGAYGIQGKGAL
                     FIEKIEGDYYNIVGLPLFKLSQMLKHFDITIL"
     gene            9792..10487
                     /gene="radC"
                     /locus_tag="PK768_00280"
     CDS             9792..10487
                     /gene="radC"
                     /locus_tag="PK768_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQA59442.1"
                     /translation="MGNNILIKDIPQEERPRERLLRYGAQVLSDAELLAVLIRTGTRS
                     ESALVLAQRILKGDGGKSGLTYVIDATVEELSKIKGIGTAKAVQIKAAVELGRRISSY
                     SGRQQVTITSPLDVKDLLMEEMRFLEKEHFKAILLNTKNHVISIEDISIGSLNSSIVH
                     PREVFKPAIKRSSASVILVHNHPSGDPTPSREDIEVTSRLVNAGNVLGIAVLDHVIIG
                     NNSILSLKEQGLM"
     gene            10536..11567
                     /locus_tag="PK768_00285"
     CDS             10536..11567
                     /locus_tag="PK768_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQA59443.1"
                     /translation="MRFLNIFSKDMGIDLGSANTLVHVKGKGIVLKEPSVVAISRDNG
                     AILAVGEEAKQMIGRTPGNIVAIRPMRDGVIADFDVTESMLKYFISRALRSRPFIKPR
                     VVVGVPSGVTEVEKRAVIDATLQAGAREAYLIEEPMAAAIGAGLEVHEPAGNMVVDIG
                     GGTTEVAIISLGGIVTSKSIRVGGDEMDDAISYYIKKEYNLMIGERTAEQIKINIGSA
                     APGVKEESMDIRGRDLVTGLPKTITITADEIHSALAEPVNNIVEAIKVTLEKTPPELA
                     ADIMDRGIVMTGGGSLLSGLDKRVNSETGMPVHIAEEPMNCVAMGTGKVLEELDLLKR
                     VLISPKKVG"
     gene            11575..12420
                     /gene="mreC"
                     /locus_tag="PK768_00290"
     CDS             11575..12420
                     /gene="mreC"
                     /locus_tag="PK768_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQA59444.1"
                     /translation="MFLRRLLKNRRLLIPIVFLIIAIAVVNITSKNQYVFKNIEMPLA
                     TALSPAQRLLSDLGFGIKETINAIPQFFYLKQENEALKEQVAELLQYKQYITEFQIEN
                     QELRNLLGFRERSFQYDLEAAEVIARDPSNWFNVISIDKGERHGIQKDMAVVTDKGLA
                     GYVISTTHSTSKVMLITDERSSVSAMIQRTRDNGVVKGSIDPAPSGYLKMVFLPHDAN
                     LVKGDIVISSGLGGLIPKGIVIGEVEEANKESYELMQHAIIKPSVDFLKLERVFVIKG
                     EKEAN"
     gene            12420..12923
                     /gene="mreD"
                     /locus_tag="PK768_00295"
     CDS             12420..12923
                     /gene="mreD"
                     /locus_tag="PK768_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQA59445.1"
                     /translation="MKALCYILVTLALFLMQTTMLNLIWTFAIKPDLPLVFALCIAMA
                     DGEKAGAAIGMLNGFLEDVFFGRFIGLGTIAKTAAAYLIGYGSQNLYKGRTIITMALT
                     FVGTVVFNLFFVIVAYLTGELVYPWYQFFSITIPTALLNMLISPLIYRGVVKIERFID
                     FYFDIKY"
     gene            12941..15010
                     /gene="mrdA"
                     /locus_tag="PK768_00300"
     CDS             12941..15010
                     /gene="mrdA"
                     /locus_tag="PK768_00300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQA59446.1"
                     /translation="MRAKKLQKRLNVLLGIVIAIFVVLTISLSALQIVKGDEYEKLAE
                     ENRIRIIPITAPRGVFKDRNGRELVNNRPSFTVSYMSVKTERETQEEVFAILRDILDI
                     PVYTEVSNEKRTVNEDKRIYLSKLPVVDKNGDGKLDYSDITIVEESTGKLLSPVKIDF
                     AAGVVTLDSEPGTAVLLSYNYDTLKNKLRGQGYRPVRLKTDVDFQTVAEIEERRLPGV
                     VIEVEPMRNYLYGNTASHIFGYVGEISQDELESSKDKGYRPGDMFGKTGLEKELESYL
                     KGVDGGQQVEVTASGKPMKVLGQKEPISGHTVNLTIDIKLQQIAENKLKEQLIKLQTD
                     RYNPFPNAKRGAVVALNVKTGEVLAMASIPDFDPNMFARGITQKEWEEISSNPLNPMV
                     NLAISGTFPPGSVFKMVTATAALEEKVTNERESLRCSGVYWTILPKKDWKPGGHGTVN
                     MVRAIGESCNIYFYEVGRRLGIDNIEKYARQYGLGSLTGIELPGEKAGNVASREYKKN
                     TFTRPDDKIWYPAETLDAAIGQGYNSFTPIQIANYVAAIANEGTWMKPHLIKSIVAPD
                     GNTVLEKNPEIGGQIDVSKSTFDVIKRGMRAVTLPGGTAYGVFADFPIAVAGKTGTAE
                     WDVTKDPHGWFVAFAPYEDPEIAVAVFIEQAGSGGSTGGPVARAIFEEYFHLTDVEKT
                     EDYLIQP"
     gene            15123..15734
                     /gene="minC"
                     /locus_tag="PK768_00305"
     CDS             15123..15734
                     /gene="minC"
                     /locus_tag="PK768_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HQA59447.1"
                     /translation="MGNKPVIIKGTKDGITIILEENSNFEDVEKIIYEKLQSGRNFFA
                     GGKVYLKSKKGFVSEEEYKKLQEILTNFGMSLQEADSPKTLIFPKPNRSRVLLLKRTV
                     RSGQKISYRGTVVILGDANPGSEIVATGDILVMGALRGMAHAGAEGDVSAIVAAFRLK
                     PTQLRIAEIISRPPEDKEDLPQFPEIARLKDNIIVIEPYNTLK"
     gene            15761..16570
                     /gene="minD"
                     /locus_tag="PK768_00310"
     CDS             15761..16570
                     /gene="minD"
                     /locus_tag="PK768_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HQA59448.1"
                     /translation="MGEVIVITSGKGGVGKTTTTANLGTGLALYGNKKVVLVDADIGL
                     RNLDVVMGLENRIVYDLVDVANGMCRLKQALIKDKRFENLYLMPAAQTKDKTAVTPEQ
                     MKKLCEDLKQEFDYVLVDSPAGIEQGFKNAVAGADKAIIVTTPEVSAVRDADRIVGLL
                     EASGFEEPKLVINRLRPDMVRRGDMMDIDDMIDILAIELLGVVPEDEKIVISTNRGEP
                     AVADESSKAGKAYRNMVSRLLGQDVPLMTMENVEPSFFDKLRMFFGLKART"
     gene            16593..>16761
                     /gene="minE"
                     /locus_tag="PK768_00315"
     CDS             16593..>16761
                     /gene="minE"
                     /locus_tag="PK768_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HQA59449.1"
                     /translation="MDFLKIFGRESVASKDIAKERLRLILVHDRANVSPKFLEMIKSQ
                     LIDMISNYMEID"
BASE COUNT         4871 a         3248 c         3918 g         4724 t
ORIGIN      
        1 aaaagatttc gccactggtg aaactttaaa tctcaattct gatcaggttt acgtggcggc
       61 tagcacaata aagttacctt tagttctgta tatatatgaa ttggcggcac aaggcaaaat
      121 aaatcttgat gcaaaactca cttatacacc ggaatattat gcacagggta ccggtattct
      181 ccaaggagaa cctttcggcg ggcaatatac aataagggaa ctttcgcggc tctccatgga
      241 atatagtgac aatgtagcct ggaagatgct gctagatttc atgggccaag acaaattaac
      301 ggcgtatgag aagtctttgg gtgccaaggc taccggtggc gataacggac tttatataac
      361 aactcctgaa gatatgggta tatacctgga aaggttacta aatttcagcg aagaaaagcc
      421 tgaatatggg aatgaggtcc tccattatat ggctaattcc atcttcagtg aaggaattcc
      481 tcaagatctg cccgatgata ttgtggttgc tcacaaaatg ggtgcattga atgataaatt
      541 tcatgatgta ggtattgtct ttggaaatcg gccttatata attgccatct ttaccgatga
      601 ggcatgggaa gaggtatcct tacagaccct tgccgatgta tcgcgtattg tatataattt
      661 ccaggaatca taaagtaagg tataacatat atatttctgt ttgacaacag agcccttatc
      721 ttattcaact taagataagg gctctacttt aggctataat ttctccttca atgctgactt
      781 ccattgattt tatggggatg tttttccctg agcgccgtaa ggattcctct actatcttaa
      841 ccataccgaa gcagcaggga acttccatat gagctattgt tatgcttttt atattattat
      901 tttttaagat tgctgagagc ttttctacat agtgatttcc gtcatcaagc tttggacaac
      961 ccatcactac agccttgccc ttcagcattc ccagatggta attgggatag gcaacagcta
     1021 cgcagtcagc tgttatcaac aaatcagcgt tctcaaaata gggggcctgt actggaacga
     1081 gcattagctg caccggccac tgtttcagct gggatttgat ggatattgtg acatcgccgg
     1141 tatcgcaagt agaagcggtt ttattagtct gcctcatgtc gatagctctt gagccggggc
     1201 agctaaaaac ttggctactt ccggggcagg aaaagccctc ctcatcaaaa tcttctcccg
     1261 agcttttcag gtattcttct acagcctctt catcaaattc ttccgcttca cgttcaataa
     1321 ttgtgatggc accctgaggg catgagccaa gacatgctcc aaggccatcg cagtacttgt
     1381 ctgcaaccag gcgggccttg ccgtctataa ttttaattgc tccttccgca catgatggaa
     1441 tacataggcc gcagccattg catttatctt catcaatatg aactatactc cttactgcca
     1501 tagtatttct cctttcttag tcatctacaa caattatagc aggaagaaaa agatttaatg
     1561 tgacaaagct cacaaccctt gatttataaa aataggaaag ttccagtttt tttttcataa
     1621 atatctaggg gagggaaata catgaaaaga aaacatataa ttttactttt cttaatttca
     1681 atcctcatca tgtggatttt attttctcga tttagtaggg tcactattag gcagctagac
     1741 gatctgggca ccgtgaagat tactatcaat ttttacattc aggtagagca ggaaggcata
     1801 gaggataaga taaaactttc ttccgaaagg ccgggcactg aaatcataaa aaatcttata
     1861 tggattgatg atacgacctt agagatactt gccatggaaa aagcccttcc aaaaggcttt
     1921 aatacagaat tgaaagtagg tccgttaaag acatccatac ccggtttata taagctggtt
     1981 aaggttaatt ttagtgcaga gatttcacca tttctaaccg gcatgtcgtc cattgccgcc
     2041 agcacgggcc ccattgaact ggacttttcc acgccgataa aaatacagga tttaagaaaa
     2101 ggccttgttg ccgattttga ttatgtctta agggaaaggt accttataga cgaaaacgga
     2161 aaggtattta aagactgtag cagctttgaa ctagtgccca aggagcctct tgtcccgggg
     2221 aagcagtatg aagtaaaatt cgatgggctt ttgtcaaact ataccgatgg tgcaagcaaa
     2281 acagcttttc acaggatttt tacagttgcc gatgtgccga aagtagttag aacacagccc
     2341 caagacagtg agagggatgt taaactctac gaccaggttt tagttgaatt tgatgaagat
     2401 atgaaagaag ttgatataga gattgaaaaa atgtcgggcg atataaaact tgaaggcaag
     2461 accgccgctt ttaagcctca taccgtattt gtccccaata caacataccg tgtaagggta
     2521 acaggcaaat ccacctttgg cgaggcaatg gccccctaca gctttgagtt tactaccgtg
     2581 gatatgggag ataaatggtg ggtggaaatt aacttaaggc ccattcaaaa ggtgaccgtt
     2641 tttcgaggca ataaaattgt caaatccatg atagcttcag cgggccttcc agatgaggaa
     2701 aatcgaactc cccggggctt tttcacctta aaagatagaa gagaatactt ttggactgag
     2761 aggatacagg aagggggcct ttactgggtg agaataacag acaattattt aattcattcg
     2821 gtgccacgag ataaagacaa tgaaataatt gaggaagaat ttaaaaagct gggcattcct
     2881 gccagccacg gctgtgtaag acttaaagac gaagatgcaa aatggtttta tgaaaccgtc
     2941 cccagcggga cactggtgat tatacacgac tgatgcaggg aggtaagcaa tcgtgaactt
     3001 tattttttcc ttattcatct taacaggaat aatcgtggct gcagcaaatg gaagaataga
     3061 tattattaca accgctgcca tgacttcagc agcaaatgca gtgcaacggg ccattaccct
     3121 aattggcatc attagcttat ggttgggtat agcgagggtt gccgaggaat ctggcctaat
     3181 caatattttg agccggctga tagcccccct atttcgctgg ctttttccgt caattccaaa
     3241 aggtcatccc gccttgggct gcatcttaat gaacttaagt gccaatatgt tgggttttgg
     3301 gaatgctgca acacccttcg gcctgaaagc catgcaggaa ttacagcgac taaatgacaa
     3361 accggatact gccacggagg ccatgtgcac atttttagct ataaatacat ccagtgtcac
     3421 aatagttccc gctacgttaa tagcattgag ggcgtcgaca ggttcacaaa atccctctga
     3481 aataatggga gcagttttgt ttgccacgac ctgctcaacc atggcggcaa ttttaggcga
     3541 tattgtaatt agaaatctgt atcgcaggag gcgttcgtat tgacagcaga aatgttgggg
     3601 cagggctttt catggtttat tcccgggttg atttttgtag tttttttata cggtcatttg
     3661 aggggggtaa aagtatttga agtttttgta gagggggcac atgaaggttt catgatggcc
     3721 ataaagctaa taccctatct catcggcata tatgttgcaa ttgggatttt tagagattca
     3781 ggagctgtag acatacttgt gacgctacta tctcccatct tgagggtaat taaagcgcct
     3841 accgatgcct tgcttttgtt gattgtaagg agcctctccg gaccggcggc actgagcatg
     3901 accatggaaa ttttcgataa gcatgggccc gattctttta tagggagact ggcatctacc
     3961 atgctggggt caagcgatac caccttttac atcattgcgg tatactttgg ttccgtaggt
     4021 gtcaggaaaa cccgctacgc catccccctc ggattactcg cagattttgc aagttttgta
     4081 gcctctgtat atatagtgag aaaaatgttt atataaaaaa gctcccttgc ataatacaag
     4141 gaagcatact gcctacctta ggcgtattcc tgcgctcatt gcgcctataa attcattgtc
     4201 tttaaaaatc ggtgttgcca ccgttattgt tggcagtccc gtaagggccg aaatatgcat
     4261 tttagatgta taatcttggc cgcttttggt tgccttaaac caatccctaa atgaaaagtc
     4321 agttacattt gaaggattag aatttgcgat actccttccg gtatggtcca ccgtgattat
     4381 tcctgtaaag ttgggaaatt cttttttcag tttgtcgaaa accttatggt gttgctcttt
     4441 gtccattgac agaatttctt tggattttac ccaatgtttt agtaaattca atacttttgt
     4501 ctttatttct gaggagacgt cgtattgcga atcttttaaa actttttcta catagctata
     4561 tatttcatca gctactttca tgagacttgc ggtagtcgcg tttatgttgt gcattactgc
     4621 agcttgttct tccgagactg cggcagtttc ctggaaagct gcagatatgt cctgtgtgag
     4681 ggatgaaaag tcttcaattg ttttcttgat attgttggca gctacggcct catcattggt
     4741 gagattattt atgtggtcca tagattcaag ggtatcttca attacctgtg aggttgtctc
     4801 tagagattgt tttgcaattt ttgcatgctg tgcttcaact tgaaccttga taaaaccatc
     4861 tactatttct tcggataatt tgactattct gtcgctgata ttttgcaccg tgtcttttat
     4921 ttcactggcc gcaatttgag cgttcattga aagtttgcgt atttcatctg ctacaactgc
     4981 aaattgcctt cccatgtcac cggctcgagc ggcttctatc gcagcattta atgaaagcag
     5041 atttgtttgc tgggcaatag tttctaccga gctgataatt ccgcttacct tttcagcttc
     5101 ttttaccaat gcatctactt ccacagcagt gacgctgttt tgctgggaag taagttcgat
     5161 ggaattcacc agcttttcta tcaattggga gctttcctgc agtgattttt gcacttcaat
     5221 attgcccgaa accacctttg cggtttcgga ggcgatcagg ttggaaaggt cgctgagctt
     5281 tacaatctcc gtcacagcat cgctgcatgc gctcacctgg tcttccgtcc gttcagctat
     5341 gtggctgatg gtttcggcaa tttcctggga agatttggcc gatgcatcgg tttcagaata
     5401 aaacttttga catagctctt ttaattgttc tgaagcgatt gacagttctc ccacaagatg
     5461 cctcgtgttt tgcgtcacat tggcaatttc ctggctgaga ttgccgacaa taccgccttt
     5521 tatgtcatca aatacttgac ccaagacccc ctcgttaaat tccttgaggc ggcttaccag
     5581 cttggtaagt ttgtcatttt ccatttttct atcgatcagg tatatggcca tgtagaatgc
     5641 aataatcaag agtgagataa tgatgtagct agggttcatc agcccaagtt gccaaagtgt
     5701 cgtaaagagt actcccaaaa agataaggct agctattata aacatttata cccacagaat
     5761 acaaacaggc attgtaaata ccagttcgta tcaatgcgcc tcctttcgtt aaaatatttt
     5821 gactttatga tagagacccc tgcatcaggg caggagccgg ggccccttac agaagcgact
     5881 taacggcttc tattgtagcc tctacgtctt ccccttttat ccaataatgg gttacaaatc
     5941 tcactatcgg tgagcttccc ccatttacac gaatcccttt ctcataaagc tttgcggcaa
     6001 attcaccggc agtcatttta aggccgctaa tatcacatat gacgatattt gtctggaccg
     6061 tatccatatc tatggtaatg cctggtatct cggacagtcc ttgagccaga aacctggcat
     6121 tttcgtggtc ttcggcaagt cttgaagtca tcttttccaa tgccacaatt cccgctgccg
     6181 ccaaaaagcc cgcctgtctc atcccgcccc ctaacatttt tctaaattta cgggctctcg
     6241 ctataaaatc actcgttcct acaattactg acccgaccgg ggcacatagg ccctttgata
     6301 agcaaaacat gactgaatct gcgtgctttg caatttcttt gacggggata ttgagataag
     6361 tagctgcatt gaaaatccta gctccgtcca tgtggaccgg gatatcatgt ctttttgcaa
     6421 tttcataagt acttttgagt atgtccagag ggattactgt accgccggca cggttgtggg
     6481 tattttccaa gcagatgagg ctggtctttg gcaagtggat atcctcatca cgtatagcag
     6541 cttctatgtc tgctgggtcc attatgccat gttttccggc tatgagcttg gcctgcacac
     6601 cggccagcct tcctatgccg ccaacctcgt aagttatgat atgacttttt tcctctagaa
     6661 tgacctcatc tcccggctga gtatgggtca ttacacagat ttgattgccc tgggtaccac
     6721 ttgtaacaaa cagggctgct tcctttccca acatttgggc agccatctct tccaggcgat
     6781 tgatggaggg gtcttccccg tatacgtcgt cgccaacttg ggcattataa atagcatcac
     6841 gcatttgttt tgtaggtaaa gttaccgtgt cacttcttaa atctatatac ttcacagcta
     6901 tggcatctcc ttttaagttg tatattttat tatacattct ctataaatag taaaaatcct
     6961 tttataaaaa tcatttataa ggagaaaaaa ggaaggtttt tttgctccaa acatagaata
     7021 tatattttaa cgatatttta ttaagaggag ggtgtttatg agcggcaaaa cttttatcag
     7081 tgaatcggta tttgttgaca tagctaagga agctatgcgc aaagttgagg aagtttataa
     7141 acaggagaaa aagggcggcc tcgcaggttt taccaggatg tttgtagaca ggttcacacc
     7201 ccagatatcg gtaaagaaaa ccgatccagc agaatttgaa gaaacagctg gcagcgtcgc
     7261 gttcgaggtc aaaatcagcg tggtatacgg agtaaagata ccggaagttg cagataaagc
     7321 cagggaaaaa atcatcagtg atgtggagac attgacgggc tattctgtag aaaaagttga
     7381 cattgtcgtt gaaagagtta taaaacctga agaaatacaa gaagaaaagg ccgagcaaaa
     7441 gactgaagac taaagctatt gctccggagt tcatatctaa tctaggaatc gtattaacag
     7501 gacgctaata gtgaaggagg tatagataca tggccggatg ctgcgatcat tgtaattcag
     7561 atattatcct gaacgtttcc ggtatgtcat gcggccattg caaagcagcc gtggaaaaag
     7621 cagttggtgc actgcccggc gtttcaaagg ttgaagcaaa tgtagaagaa agcaaagtag
     7681 aaatatccta taatcctcaa gaagtaagca ttgacgatat aaaacaggcg atagaagatg
     7741 caggatatga tgttgaaggg taagattctt aatgagccaa agaagcccta tccctcgttt
     7801 gagcacaaaa gagaatatat cgataattaa aaagaattcc ctttaaggta aagagccctt
     7861 tgcaacaaaa atgtgtaatt gtgaagggct ctttacctat ttttatagtc aaattttcca
     7921 cttgttttgg atggaataaa atgttacact aattgtgtag attcaagccg agcgaggatt
     7981 ttccatgatt accaaaggtg ataaaatact atttttttct attgtacttt tatctctaat
     8041 aatgttcaca gcttttcaag tttacggttt tgcaaatggc aaaacttacc ttttaataga
     8101 ggtaaacgga aaactttatc aaaaggtctt actgggaagg gatgggccaa atctaacaat
     8161 accggttccc gtgactactg gtgagaatgt tgttgaagtt gacgcagacc gtgtaagaat
     8221 gctatatgca gagtgcccgg acggagattg cctgagacag ggatggatca gccgccctgg
     8281 tcagatgatc gtgtgccttc caaataaaat ggtcatagct atacaaagtg acaaatatat
     8341 aagtgatgat atagatgatg taacctttta atttcctcgc ttaaaggatg aatgattttg
     8401 ctaaagcacc gcaaaataat atttctttcg ctgctcgtgt cttttggcat agtgctgcac
     8461 atcattgaaa atatgattcc agtgcctttt ccggtgccgg gagccaaact tggccttgcc
     8521 aacattattt ctctaattgc cataagtttt tacggtttga gagaagggct tatggtaagt
     8581 gtcctgcgga gtatcatagg gaccttgctg gcaggttccc tttcaagtct gatatatagc
     8641 ttatcaggtg ctattatgag cactattatt atggcttttg catataagcg cttcggcaat
     8701 gtttttagtt tggtgggaat aagcttttta ggcggggtaa cacacaacat aacgcaggtt
     8761 acggtggcca gccttgccct ttccacttta gggctgtaca tttacctgcc ttttttaatg
     8821 attatcggct tatttaccgg cctattcacc ggcctggcag ctcagtttgt aaaacaaaat
     8881 cttgatacca ctcttataaa actgggtata ttgagggagg ataaaatcaa atgaaacgta
     8941 gttatcgcag tccacttgta ttggttataa tattattagt aggtctagta attggaagtg
     9001 tgttaggcca ggtattaggc agttatgccc ctattttaaa tcagggcagc agtataggtt
     9061 tgtccacaac caatctggat ttaggaatta tcaatgtcac cttcggtttt aatgtgagtt
     9121 taaatttagc tggggcccta gggcttatca tagcaattat cttttatcag cgtatgtagt
     9181 gaggttttga gatatggaaa gacctattat tttagcctcg gcttctccta gaaggcagca
     9241 gcttttattg caactaggcc tcaagttctc tgtggaacca agctaccttg atgaaaaccc
     9301 ggacactaca atggactttg gccctttagc agccaaaatg gcccatagta aagccatggc
     9361 cgttgccagc aagcacgatg aaggtctggt tataggcgct gataccatcg tggttttgga
     9421 tgacaggata ttaggcaagc ctggcacttt tgaggaagct gaagaaatgt taaggaatct
     9481 tagcggccgt tggcataagg tgttcacagg agtatccctg gttgatgctg ccacaaagcg
     9541 ccatataaag gaatttgaag agagccgggt taagttcaaa gtcctaagca gtttagagat
     9601 acaaaactat ataaaaaccg gtgagcctat ggacaaagcc ggtgcctacg gaatccaggg
     9661 caagggtgcc ctgtttattg agaaaatcga gggtgattat tataatattg ttggattgcc
     9721 tctttttaaa ttaagtcaaa tgttgaagca ttttgacata acaatccttt aagtacaggg
     9781 ggttgctgtt gttgggcaac aatatcttga taaaagatat accgcaggag gaacgacctc
     9841 gtgaacgcct gctgagatat ggagctcaag ttttaagtga tgcggaacta ttagccgttt
     9901 taataagaac cgggacgcgc tcggagtcag cattggttct tgctcagcga attttaaaag
     9961 gtgatggagg aaaaagtggg ctaacttatg tgattgatgc tactgtagag gaactttcaa
    10021 agatcaaggg tatcggcacg gctaaagctg ttcaaatcaa agcggcagta gaactgggcc
    10081 gaaggatttc atcttatagc ggacgtcagc aagtaacgat aacgagccct ttggacgtaa
    10141 aagatctcct catggaggaa atgagatttt tggaaaaaga acatttcaag gctattttat
    10201 taaataccaa aaatcacgtg atatcaatcg aagatatatc tatagggagt ctcaactcct
    10261 ccatagtcca tccccgggag gtttttaaac cagccataaa acgcagtagc gcatctgtca
    10321 tattggttca caatcatccc agcggtgatc caactcccag cagggaagat attgaagtta
    10381 cttctaggtt ggttaatgca ggcaatgttc ttggaattgc tgtgctggac catgtgatta
    10441 ttgggaacaa ttctattctc agccttaagg aacagggtct tatgtaagac aaagcacatg
    10501 ctgaaaaact gaggcagcag aaaggggaaa cattgatgag atttttgaat atattttcaa
    10561 aggacatggg aattgattta ggctcagcta atacattggt gcacgtgaaa gggaagggca
    10621 tcgttctaaa agaaccatcc gttgtcgcta tatcccgaga caatggtgct attttggcag
    10681 ttggggaaga ggcaaagcag atgataggcc gtacccctgg taatatcgtt gctatacgac
    10741 cgatgaggga tggcgttata gcggatttcg atgtcaccga aagcatgctc aaatacttta
    10801 tatcgcgggc actcaggagc aggcctttta ttaaaccaag ggttgtagtt ggcgtccctt
    10861 ccggagtaac tgaagttgaa aagagagctg tcattgatgc tactttgcaa gcgggagcta
    10921 gggaagctta tttaattgaa gaaccgatgg cagcagccat aggagctggc cttgaagttc
    10981 acgaaccagc cggaaacatg gtagtggata taggtggtgg gaccacggaa gttgctatta
    11041 tctcattggg gggcattgtt accagcaaat cgattcgtgt cggtggcgac gaaatggatg
    11101 atgccatttc ttactacatt aagaaagaat ataacctgat gataggtgaa agaacggcgg
    11161 aacaaataaa gattaacata ggctctgctg cacccggtgt aaaagaggaa tccatggata
    11221 ttagggggcg ggacttggta acaggcctgc ctaaaaccat tacaattacc gccgatgaaa
    11281 tccattccgc tttggccgag ccagtaaaca atatcgtaga agcaatcaag gtgactttag
    11341 agaagacgcc gccggaattg gctgccgata tcatggaccg tggaattgtg atgaccggtg
    11401 gaggttcttt gctatcaggc ctggataaga gggtaaacag cgagacggga atgcccgttc
    11461 atatcgccga ggaacccatg aattgcgttg ccatgggtac aggaaaggtc ctggaagaat
    11521 tggaccttct aaagcgcgtc ttaatttctc caaaaaaagt aggttgagga cgtggtgttt
    11581 ttgcggcgtc ttttgaaaaa ccgtagactg ttaatcccca tcgtgttttt aattattgca
    11641 attgcagtag tcaatataac atcaaagaat caatatgttt ttaagaatat agagatgccc
    11701 ttagcaactg cactcagccc tgcacaaaga ttactgtctg atttgggatt tggcataaag
    11761 gaaactataa atgctatccc acaatttttt taccttaagc aagaaaatga agccttgaaa
    11821 gagcaagtgg ctgagctttt gcagtacaag caatatataa ctgaatttca aatagagaac
    11881 caggaattgc gcaatctctt aggttttaga gagagaagct ttcaatacga tttagaggcg
    11941 gcagaggtta tagcaaggga tccgagcaat tggttcaatg tcatatccat tgataaagga
    12001 gaaaggcatg gtatacagaa ggacatggca gtggtcaccg ataaaggttt ggcaggatac
    12061 gtgattagca ccactcacag cacctctaag gtaatgctta tcacggatga gcgcagttcg
    12121 gtcagtgcca tgatccagag gactagagat aatggtgtag ttaagggtag tattgacccg
    12181 gcgcccagcg gatatctgaa aatggttttt ttgccccacg atgccaacct ggtaaaaggt
    12241 gatatagtta tatcctccgg cctgggtggc ttgattccta agggcattgt cataggagaa
    12301 gttgaggagg ccaataaaga atcatatgaa ttgatgcagc atgccataat caagccttcg
    12361 gtagactttc tcaaacttga acgggttttt gtgataaagg gagagaagga ggccaattga
    12421 tgaaggccct ttgctatata ttagtaacac ttgcactttt tttaatgcag acaaccatgt
    12481 tgaacttgat ttggactttt gcgataaaac cagacctgcc gctggttttt gctttatgta
    12541 tagccatggc tgacggtgaa aaagccggtg ccgcaattgg catgttaaat ggctttttgg
    12601 aagatgtttt tttcggcaga tttataggtc ttggcaccat tgcaaaaact gccgcagcgt
    12661 atcttatagg gtacggttca caaaaccttt acaaagggcg aactattatt accatggccc
    12721 ttacctttgt gggaacggtt gtgtttaatc tgttctttgt catcgtagct tatttaaccg
    12781 gtgaattggt ttatccttgg tatcagtttt tctctatcac gattcctacc gcactgctga
    12841 atatgctcat atctcccctg atttatcgag gagttgtcaa aatagagcga ttcattgatt
    12901 tttactttga tattaaatat tgatgttttg aggggttttt atgcgggcaa agaagcttca
    12961 aaaaaggctc aacgtattat tgggaatagt aattgctatt tttgtagtcc ttacaataag
    13021 tttgtctgca ctacaaatag ttaaaggtga tgaatacgaa aaactagctg aggaaaacag
    13081 gatcagaata attcctatta cagcacctag aggtgtcttc aaggatagga acggtcggga
    13141 actggtaaac aacaggccaa gttttaccgt atcgtatatg agtgtaaaaa ctgaacggga
    13201 aacccaggag gaagtctttg caattctgcg agacatcctt gatattccag tgtacactga
    13261 agtttcaaat gaaaaacgca ccgtaaatga agataagagg atatacctta gtaaattgcc
    13321 agttgtggac aaaaatggag atggaaagct ggattattca gatattacaa ttgttgagga
    13381 gagcacggga aagcttcttt cacccgtgaa aatagacttt gctgcaggtg ttgtaaccct
    13441 tgacagtgag cccggcacag cagtgctatt atcctacaat tatgacactc taaagaataa
    13501 gctgaggggg caaggttata ggcctgttag actgaaaacc gatgtggatt ttcagaccgt
    13561 tgccgaaatc gaagaacgac ggctcccggg tgttgtaatt gaagtggaac ccatgcgcaa
    13621 ctatctttac ggcaataccg cttctcatat attcggatat gttggtgaaa taagtcagga
    13681 tgagcttgag tcatcaaaag acaaggggta caggccagga gacatgtttg gcaagacggg
    13741 cctggaaaag gagttggagt cataccttaa gggagtagat ggaggtcagc aggtggaggt
    13801 caccgcatcc ggcaaaccca tgaaggtgct ggggcaaaaa gagccaattt cagggcacac
    13861 agtcaatctg acaatcgata taaaacttca gcaaatagct gaaaataaat tgaaggaaca
    13921 actgataaaa ctgcagacgg ataggtacaa tcccttcccc aatgccaaaa gaggtgcggt
    13981 tgtagccctt aacgtaaaga ccggcgaagt gctggcgatg gccagcatcc cggacttcga
    14041 ccccaacatg ttcgctagag gaataactca aaaggaatgg gaagagattt ctagcaatcc
    14101 cctcaacccc atggtgaatc ttgcaatttc aggaactttc ccgccgggtt ccgtatttaa
    14161 gatggtaact gcaacggctg cattagaaga aaaggttact aacgaaaggg agagtttgcg
    14221 ctgctcggga gtttactgga ctatcttgcc caaaaaggat tggaaacctg gcggccatgg
    14281 cactgtaaat atggttagag ctataggaga atcttgcaat atctactttt atgaagtggg
    14341 ccgcagactg ggaatagata atattgaaaa atatgcgagg cagtatggcc ttggaagttt
    14401 aacaggcata gagttgccgg gtgaaaaggc aggtaacgtg gcaagtcgcg agtacaagaa
    14461 aaacaccttt accaggccgg atgataaaat ctggtacccg gctgaaacct tggatgcagc
    14521 cattggccag ggatataata gctttacgcc aattcaaatt gccaattatg tagcagctat
    14581 agccaatgaa ggcacctgga tgaaacccca tttaataaaa tccatagtgg ctcctgacgg
    14641 caatacggtg ttagagaaaa atcctgaaat aggcggccag attgacgttt ccaagagcac
    14701 ctttgatgtt ataaagcgag gcatgcgggc tgtcactttg cccggcggca ccgcctatgg
    14761 agtctttgca gactttccca ttgctgtcgc gggtaagacc ggtactgctg aatgggatgt
    14821 cacaaaagac cctcacggat ggtttgtggc ttttgcccct tatgaggacc ctgaaattgc
    14881 agttgcagta ttcattgaac aagctggatc aggaggcagc actggaggac ccgtagcaag
    14941 ggctatattt gaagaatatt ttcatctgac cgatgtagag aaaaccgagg actacctaat
    15001 acagccttaa ccaaaaatta ccagaatctt tacagattct ggttttttta gcactaaaag
    15061 aaggatttga ttaatttatg aagaataaat acttggagtg gtgctgtttg gagggaattg
    15121 gcatgggtaa taaacctgta attatcaaag gaacaaaaga tggtattacc attatacttg
    15181 aagagaattc aaactttgaa gatgtagaaa agataattta cgagaaactt caaagcggtc
    15241 gcaacttttt tgctgggggt aaggtctact taaaatcgaa gaagggtttt gttagcgagg
    15301 aagagtacaa aaaactacag gaaatcctga cgaatttcgg aatgagtttg caggaagcgg
    15361 attctccaaa gaccttaata ttcccaaaac ctaatcgaag ccgggtgttg ctgctgaaaa
    15421 ggactgtgcg ttccggacaa aagatttcct acagaggtac ggtggtcatt ctaggtgatg
    15481 ccaatcccgg gagtgagatt gtagcaacgg gcgacatact ggttatggga gcccttcggg
    15541 gcatggccca tgctggagct gaaggcgatg tatccgcaat cgttgcagca tttcgcttaa
    15601 agccaactca gcttaggatc gctgagatta tttcgagacc tccggaagac aaggaagatt
    15661 tgccacaatt tccggaaata gctcgcttaa aggacaatat catagtcatc gaaccctata
    15721 acactttaaa atagtgggat gagtaaacag gagggataac atgggtgagg taatagtaat
    15781 tacatcagga aaaggcggag tcggtaaaac tactactaca gctaacctgg gaaccggtct
    15841 tgccttgtac ggaaacaaga aagttgtatt ggtagatgcc gatataggct tgagaaacct
    15901 tgatgtagtg atggggcttg aaaatcgaat agtctacgat ttggttgatg tggcaaacgg
    15961 catgtgccgc ttgaagcaag ccctgataaa ggataagcgc tttgagaatt tgtacttgat
    16021 gcctgcagcc caaaccaaag acaaaactgc agttactcct gaacagatga aaaaactgtg
    16081 cgaagattta aaacaggaat ttgactatgt gctagtcgac tctccagcag ggatagagca
    16141 gggctttaag aacgccgtgg ccggtgcgga taaagcaatt atcgttacaa cacctgaggt
    16201 ttccgcagta agggatgcag atagaattgt ggggctttta gaggcctcgg gtttcgaaga
    16261 gccgaagctg gtcataaatc gcttaagacc cgatatggta agacgcgggg atatgatgga
    16321 cattgatgat atgatagaca ttttggccat agagctatta ggcgtggtgc cggaagatga
    16381 gaagatagtc atatccacga atagaggtga accggcagtt gccgatgaaa gctcaaaagc
    16441 agggaaggct tacaggaata tggtaagtcg actgttaggt caggatgttc ccttgatgac
    16501 aatggaaaac gttgagccaa gcttttttga taaattgcgc atgttttttg gcttgaaagc
    16561 gcgaacctga taagtaaaag gaggaagcat aggtggattt tttaaaaatc ttcggcaggg
    16621 agagtgttgc aagtaaagat atagctaagg aacggctgag attaattcta gtacacgatc
    16681 gggcaaatgt gtcacctaaa tttttagaga tgataaaaag ccagctgata gatatgattt
    16741 ccaactatat ggaaatagac g
//
LOCUS       DAONUO010000004        15951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_2728_length_15951_cov_4.229869, whole genome shotgun sequence.
ACCESSION   DAONUO010000004 DAONUO010000000
VERSION     DAONUO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2728_length_15951_cov_4.229869"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..739)
                     /locus_tag="PK768_00320"
     CDS             complement(62..739)
                     /locus_tag="PK768_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQA59450.1"
                     /translation="METVAIIGAGRMGSFIARKLNSQYNIILIDKDLRKCGLLAKEIS
                     AIATDSYSLLALSDYVILALPADAIHQAISELKSVLQQQQVVINVSTNTEKIALDPLK
                     KLSKIAAAKIIGHAQHMASTGELPLIVIDSEDITIRNRVAKIFSLLGTVCFGDEILVK
                     NINNIASEEAIRAALNIMKRLEEINIPPEYISFAIRNVACGTMSAFALGEGGPFVQKL
                     IQKINQQ"
     gene            complement(750..1841)
                     /locus_tag="PK768_00325"
     CDS             complement(750..1841)
                     /locus_tag="PK768_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59451.1"
                     /translation="MSLIVGIAGTAKNTGKTTTTSAILNELYKINISIGLTSIGYDGE
                     EIDNITRLPKPRLFVRENTILATAQKCLCGGNAKYEILETTDIKTPLGNINIVRVVKE
                     GLAVVAGPNKGSQLRYVENRMIDDFGCKIVLVDGALNRIAPMIETDGIIIATGAARNV
                     NIDILVKETKALYDLLNLADISNDEFLHSVQADKIILFPENLNEEKKLLNYGSLIDDS
                     TVKEIIDRIEDTRIIFIPALIAESALKQLNDGMGKLWANRTLVIKDSIKLLTGGEPEN
                     LLNEINRIKENGGNVKTLRKLPLLAITVNPFYPKYRFEKDMYEPGYIDKHELLEKMRK
                     AVKVPVIDIKHEGATTLLNTLLACLEKQS"
     gene            complement(1889..3703)
                     /locus_tag="PK768_00330"
     CDS             complement(1889..3703)
                     /locus_tag="PK768_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778423.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQA59452.1"
                     /translation="MSRKKVLLAPLDPVHDIGLKMIKRGLEQNGHDTILMPPDHTQEE
                     IIKAIIDNKADVVLISRTLGYGVAEILGKFIDLAEAAGLRDRVKIGIGGMAIRPELAA
                     ELGFDAGFGPGTTVEEAVAFVEDREYIPAETGVKQEKKDITADYKYDFNNKRIEKLLD
                     TIVDGILDYVSDKTTTAIMRAELRRQIIESTSESEKDKFRREYAKLCDDVVKSFYEAG
                     KYREKTRPLSSDEVSALSKYVGDVNSRMRPMKLQHTDKNPVVFIQYGTGCPFMDIAHI
                     KSCEAWGADGVIHFDPSWGARTEGFLEGYITHEEDGSIITYENLKSIKESLAPYTLWQ
                     VRAHRGLNTPETVVIAGKIGADLTKINIAYGSLSGGTDPERLTVDAVAAIKYAAEFSM
                     PFDVVTNEELSGVPAYKAFAGMLIVSKLGLRLGGKPILQPLFCYSPEVMINGQMEDNY
                     VDFNAAKIYCLKNIVNAPIWCGAPIGFLTQTEDRVQSSLMTALHASLASSLGVDGISI
                     ASSDEAFSKGPITAASRIDTLRATQASFRFFGHANMEPTENAKKWAEEMEEGIEKVLE
                     SVVKNGSFIDALYQGLLGSREDGAYPGRAGRNSVLTKA"
     gene            complement(3717..3950)
                     /locus_tag="PK768_00335"
     CDS             complement(3717..3950)
                     /locus_tag="PK768_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59453.1"
                     /translation="MSDILEKVMDAVDLETYLVCKDEEEAEKFTLELMEKLGFKDTTI
                     VFIQHQGPGARIRARGYIYKPGDRYSWLFDQSN"
     gene            complement(3993..4745)
                     /locus_tag="PK768_00340"
     CDS             complement(3993..4745)
                     /locus_tag="PK768_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HQA59454.1"
                     /translation="MQHDKMRMLFLILKSIDNSEVPVGSGYVRNILQMQGINISEATV
                     GRILRQLDIKGYTEKIGFKGRKLTPAGKKKLRELERENTINHYGKELLNVIKVTGKQE
                     LLDLLIARKAIESQLAKLAAMYITDEEISEMQNIVKRQQMHVKEGISIADDDVEFHKI
                     ISKAARNRVLDAAMDLIRQHGQLSPMLEFIRKEVKSTVLLDHKKIFEAIASRNPELAE
                     KAMIDHIEKLERDVKRYWEIVNGVDDSSSDND"
     gene            complement(4921..6204)
                     /locus_tag="PK768_00345"
     CDS             complement(4921..6204)
                     /locus_tag="PK768_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA59455.1"
                     /translation="MPKVVVIGGGWAGTAAALSAKKAGADVVLLERTDMLLGTGLVGG
                     IMRNNGRFTATEEMIAMGGGELFQVTDSVARHKNIEFPGHKHATLYDVALVEPAVKKV
                     LIDAGVEVYTESRVKDVVGEGNKILAVITEDDRKFEGDVFVECTGTAGPQSNCTKYGN
                     GCAMCVYRCPSYGPRVSIAAKMGVKEMMAKKADGSYGAMSGSCKLFKESLSQEIVDKL
                     NSTGVAVIPVPEHSKEGSDILTKKACQQYALKEYVDNVILLDTGHAKLMSPFFPLEDL
                     RQIPGFENARYEDPYAGNLGNSIRYLALSPRDNHLKTEGLENLFCAGEKAGPLVGHTE
                     AICTGSLAGANAVRWAVGKELVQIPTELAVGDAIAYVKEQMKTEEGISKKYTFSGSVY
                     FERMKELGLYSTDVNAIKERVEKAGMTDVFNQKVV"
     gene            complement(6271..7425)
                     /locus_tag="PK768_00350"
     CDS             complement(6271..7425)
                     /locus_tag="PK768_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59456.1"
                     /translation="MGCLDVLFSPPMQGRGDNYQARYIIPYEKHLHHHGGYGRPFPGR
                     FKDVLADRGSTAGELGFYGKRQNNLSMYIVPLVGADYSVHGIKFHPTFQLTNVDPEAS
                     AEKIKKVAERHATMLTGITSLAYDTPGYGYGVKDEDGVPVLQKRLAEIAKDFDVPYVL
                     DNAWGVPFIGHDITKSGADVIIYSMDKASGSPTSGLIIGKEEFMVPIRRALGMHGDRY
                     GTTSSYGKAAYVGFDPGKEAMLGMIAALKELRDNPENLKKPVDDMYDIVKEEFEQIHP
                     KLKKGILISKSYNSRAVEVNYEKTWEGDELGFPIFSIEDMYAGSQLLQTGLSQMGVIP
                     TVAYDGNILISPGLGTTDDEGQIIDSKFRYAVKALVKLMEIIAKYSGYID"
     gene            complement(7353..7736)
                     /locus_tag="PK768_00355"
     CDS             complement(7353..7736)
                     /locus_tag="PK768_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59457.1"
                     /translation="MAKFNKKFQMYQYLQANIANIYAEAKKAADEIGIPEDLRGKFGL
                     TGAISGCPSPLSRNIREAVEKGAQEVIPLSKLVDEIRSIVKDVYGDEYDAAPISTCEA
                     GIWVALTFYFHHQCRGAVTIIRPVI"
     gene            complement(7752..8210)
                     /locus_tag="PK768_00360"
     CDS             complement(7752..8210)
                     /locus_tag="PK768_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQA59458.1"
                     /translation="MSYEKKLQELGLALPEPPKPVAAYVPGVKVDKYIYTAGQIPFVD
                     GQLKYKGKVGDEVSVEDAYEAAKICALNCLSIVKSLAGSLDNVEKIVKVVGFVNSADG
                     FNQQPQVINGASELLGQIFGEAGAHARSAVGVNQLPMDATCEVELIVKLK"
     gene            complement(8251..8673)
                     /locus_tag="PK768_00365"
     CDS             complement(8251..8673)
                     /locus_tag="PK768_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59459.1"
                     /translation="MADPYKTGMFKTNPYAKKKALKGNLVVVLDGKYEERGLQLIPQP
                     SRCLVADEVHELILTDEAKKPGDVVNKIAYIGFFVVRESTVITVGDEVKINGQVIGNI
                     AGFDETHMPNHYNIVIYGEERTSGNERNLDLDDEVVIG"
     gene            complement(8775..9908)
                     /locus_tag="PK768_00370"
     CDS             complement(8775..9908)
                     /locus_tag="PK768_00370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQA59460.1"
                     /translation="MTKKLAVVLFIFCYFATGYSACSKAENETLPLSAKAFVLMDAVS
                     GRILLEKNAQEKMPMASTTKIMTAVIALEKGDLNSRIKVSQKAASMGGSSFYLKSGET
                     MTLENMLYGLLLPSGNDAAVAIAEYIGGTEEKFVQMMNKKALELGALNTHFTNPHGLD
                     DPEHFTTAKDLAVIAKHAWKYNKFREIVQTKTKEIKDGNFARQISSTNRLLWQFDGAD
                     GIKTGYTGNAGKCLVATANKKGFRLISVVLGSTDHFQDSQRLLDYGFENYYLKPIVRK
                     NEICTSVTVEKGVIKSVGLIAEDNILLPIKEGEKVNYYLIAPKKISAPVFKGESIGQL
                     QIYIDNSFVESVNLVAAGDVRLRKYYDLVNRIFRFWMKWETNI"
     gene            complement(10068..10460)
                     /gene="ytfJ"
                     /locus_tag="PK768_00375"
     CDS             complement(10068..10460)
                     /gene="ytfJ"
                     /locus_tag="PK768_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HQA59461.1"
                     /translation="MDEHPIQSLIKTSMESIKEMVDVNTIVGDAVETQDGSVIIPVSR
                     VCFGFAAGGSEFDVKASKDNDKSEKPKLPFGGGSGAGVSVQPVAFMVVGQGQIRLLSV
                     KQNTTVERMIDIAPQIIDQINMLLKKHQ"
     gene            complement(10492..11157)
                     /locus_tag="PK768_00380"
     CDS             complement(10492..11157)
                     /locus_tag="PK768_00380"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HQA59462.1"
                     /translation="MIYLLLILAFSLFFFLQVRFSIVLIKKIQDEYIKFEMDSYMSKV
                     FLEIPEMKLTVKSFVPILSFEYKVGSKSTAKAKSGKIVISPLRDIFRKIIDSMKFFVT
                     YFYTFKILIIRLLKKVNLVYLNINIAFGSENPAFTGVLAGTVWTIISQSLGLLSVWLN
                     FEGAKVKVVVTPDFFKHKSFQMKIDCIFSLRVGYIIIASFIVAWYLMIYKIRSLTKKH
                     QHS"
     gene            complement(11216..11728)
                     /gene="scpB"
                     /locus_tag="PK768_00385"
     CDS             complement(11216..11728)
                     /gene="scpB"
                     /locus_tag="PK768_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQA59463.1"
                     /translation="MCKVELERITGIIEGILFTSGNPLSIDEIAHALNITNKQVLECV
                     KQLEIEYNKPCHGIMISIVGNRIRLTTKAEIFPYIERIFKPKVRSQLSKAALETLAII
                     MFKQPITKSEIESIRGVNCEKAISTLLEKNLICEKGRLSVPGRPILYVTTEECLDYFG
                     LKSIDEISIE"
     gene            complement(11728..12465)
                     /locus_tag="PK768_00390"
     CDS             complement(11728..12465)
                     /locus_tag="PK768_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQA59464.1"
                     /translation="MALNVKIDAFEGPFDLLFHLIEKNQIDIYDIPIAELTEQYLKYI
                     QEIKESRLDTASEFLVMAATLLSIKSKMLLPKPKHEVVSELAAAEDDTADPRSELMYK
                     LLEYKKFKEISMVLKKKEQEQELLFKKPPEELSSMWANEIELADISFEDIKLAFKRVM
                     SQEKVEERIAKVSKDPLPLFRKVIEIYKMLRQKSKVFFSRLYDKRTPKIEIITTFLAV
                     LELVKMKRINAVQEKQFGEIVITCREV"
     gene            complement(12482..13465)
                     /gene="trpS"
                     /locus_tag="PK768_00395"
     CDS             complement(12482..13465)
                     /gene="trpS"
                     /locus_tag="PK768_00395"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQA59465.1"
                     /translation="MKKHVIMSGMRPTGRLHMGNMMGALTNWVKLQDDYDCYFVVVDW
                     HALTTEYSSTEEIKENIRQMVIDWVSVGLDPEKCNIFVQSNVKQHAELHLLFSMITPL
                     SWLERCPTYKEQLKQLENKEINTYGFLGYPVLMATDILIYKADRVPVGKDQQPHIELT
                     REIARRFNYLYKPVFPEPEALYTEFMVLPGIDGRKMSKSYDNHIEISASPKTIMEKVR
                     MMITDPAKIRKNDPGHPEICNVFAFYKVFDQKDNVAELKEICEKGLIGCVECKKKLAS
                     IIIDKMEPIHQKRHELEQNPSIIDNILDIGAKRARIVAERTLEEAREAMKI"
     gene            complement(13483..14106)
                     /locus_tag="PK768_00400"
     CDS             complement(13483..14106)
                     /locus_tag="PK768_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQA59466.1"
                     /translation="MTHYIGPEMLLRIPALLIAISFHEYAHARVSYSLGDPTPKWRGR
                     LSLNPLVHLDPIGLIMLWLFRFGWAKPVEINPSYYKEPKKGTILVSLAGPMANLILAF
                     IAMIILKLDILRIGVLDSFIYILFLYNLTLAVFNLIPIPPLDGSKILAGLLPARQAYE
                     FSKLEAYGPFVLILLVYFGLLGKILNPLIIFIHRGLDVLSNLALLRF"
     gene            complement(14229..15563)
                     /gene="lysA"
                     /locus_tag="PK768_00405"
     CDS             complement(14229..15563)
                     /gene="lysA"
                     /locus_tag="PK768_00405"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQA59467.1"
                     /translation="MLLGTCRVNKLNHLEIGGCDAVELAKEFGTPLYVMDENLIRENC
                     RTYLNSIRSLHENTDVIYAGKAFLTIEMCRIIEEEGLCLDVVSGGELYTAIKAQFPAK
                     RIYFHGNNKSYDELKMAIEYGVGRIVVDNFYELELISDLSKKYPAKNIKVLLRLTPGI
                     EAHTHDYIKTGQLDSKFGFGMENGQALLAVESALSIKGLKLMGFHCHIGSQIFEEEPF
                     KDAAQIMVKFAKTVNDKYGFNTREIDFGGGFGIKYLETDRPLPVKEYIHALVTSVKKC
                     CQNYDVPLPKISIEPGRAIIGEAGTTLYTVGAIKNIPGIRKYVSVDGGMSDNIRPALY
                     GAKYTALIANKAASPKEERVSIAGKCCESGDMLIWDIDLPKIDPGDILAVFCTGAYHY
                     SMASNYNMLPKPAVVFVKNNTARLVVRRQTYEDLLSNEIYLPPAKKVYTASM"
     regulatory      complement(15644..15822)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         4375 a         3554 c         2651 g         5371 t
ORIGIN      
        1 tgcttttatt gtgattacaa aaccaagttg aaaaaaactg attcaagatt gaatcagttt
       61 ttcactgttg atttattttc tgtatcaatt tctgtacaaa aggtcctccc tcgcccaaag
      121 caaatgcact catggtacca catgccacat ttctaatggc aaaactaata tattcgggag
      181 ggatgttgat ttcttcaagt cttttcatta tgttcagggc cgctctaatt gcttcttcac
      241 tggcaatatt gtttatattt ttaactaata tttcatcgcc aaaacataca gtacccagca
      301 gagaaaatat cttagctact ctatttcgta tggtaatatc ttcactgtcg ataacaatta
      361 aaggtagttc accggttgag gccatgtgct gtgcatgccc gataatttta gcggcagcta
      421 tcttgcttaa tttttttaag gggtcaagag ctatcttctc cgtattagta gaaacattga
      481 ttactacctg ctgctgctgc aatacggatt ttagctcaga tatagcctga tgaattgcat
      541 cggcaggcaa tgccaaaata acataatctg atagagcgag aagagaataa ctatccgttg
      601 ctatggctga tatttccttt gccaataggc cacattttct caaatcttta tcgattaaaa
      661 taatgttata ctgagaattt aatttacggg caataaagct gcccatgcga ccggcaccta
      721 taatggcaac tgtttccata ttcagccccc taactctgtt tttctaaaca agctaatagg
      781 gtatttaaca aagtggtagc accttcgtgt tttatatcga ttaccggtac ttttactgcc
      841 tttcgcattt tctccaataa ttcgtgcttg tcaatgtagc ctggctcgta catatccttc
      901 tcaaaacgat actttggata gaaaggattt accgtaattg ccaataaggg aagctttcgc
      961 aaagtcttta cattgccgcc gttttctttt attctattga tttcatttaa tagattttcc
     1021 ggttccccac cggtgagtaa ttttatggag tctttgatca ctaaagttct gtttgcccat
     1081 agtttgccca taccatcgtt gagctgtttt agggcacttt ctgctatcaa tgcaggaatg
     1141 aagatgattc gcgtatcttc aatcctgtcg atgatttcct ttaccgttga atcatcgata
     1201 agagaaccat aatttaacag ttttttttct tcattcaaat tttctgggaa aagtattatt
     1261 ttgtctgctt gaacagaatg aagaaattca tcatttgata tatcagccaa attgagtaag
     1321 tcatataaag cctttgtttc ctttactaaa atatcaatat tcacgttgcg ggcagcacca
     1381 gttgctataa taatcccatc cgtttcaatc atgggtgcta tacgatttaa tgctccatca
     1441 actaaaacaa ttttgcatcc aaaatcatca atcattctat tttctacata tctcaattga
     1501 ctgcccttat ttggtcctgc caccactgcc aagccttctt taacaactct tactatgttt
     1561 atattgccca ggggcgtttt gatatctgtt gtttccaata tttcatactt tgcatttccg
     1621 ccgcagagac atttctgagc agttgccaaa atggtgttct ctcttacaaa taaacgaggt
     1681 tttggtaatc tggttatatt gtcaatttct tctccatcat agccgatact tgttaagcca
     1741 atggaaatat ttatcttgta caattcattt aaaatagcag aagtggtggt agttttcccc
     1801 gtattctttg cagttcctgc tatgcctact attaagctca tcattaaccc cctattaggc
     1861 gaaatcaaaa caccgcatgg tgaaattttt acgccttggt caacaccgaa ttcctgccag
     1921 ctcttcccgg atatgctcca tcttcacgac ttccaagtaa accctggtat aaagcatcta
     1981 tgaaactgcc gtttttcacc acggactcta atactttctc aatcccttct tccatttcct
     2041 cggcccattt tttagcattt tccgttggct ccatgttagc gtgaccaaag aacctaaatg
     2101 atgcctgggt tgccctcagt gtatcaattc tggaagctgc ggttattgga cctttagaaa
     2161 aggcttcatc agatgatgct attgaaatgc cgtctactcc taatgaagat gcaagggacg
     2221 cgtgaagtgc tgtcatcagt gatgactgaa ctctatcttc agtttgggtt aagaatccaa
     2281 tcggtgcgcc acaccatatt ggtgcattga caatattttt taggcaataa atttttgctg
     2341 cattgaaatc cacgtagtta tcttccattt gaccattaat catgacttcc ggtgagtagc
     2401 aaaatagtgg ctggagtatt ggtttaccac cgagtctcaa gccgagtttt gaaactatta
     2461 gcatgcctgc aaatgcttta tatgctggaa cgccggaaag ctcttcattt gtaactacat
     2521 caaagggcat gctaaattca gctgcatact ttatggcagc cacagcatct acggtgagcc
     2581 gctcgggatc agttccacca cttaatgatc cgtaagcaat gtttattttt gtcaaatctg
     2641 ccccaatttt accagctatt accacggttt caggggtatt caatcctctg tgtgctctga
     2701 cctgccagag ggtatacggc gccagggatt ctttaatact ttttaggttt tcataagtta
     2761 ttatagaccc gtcttcctcg tgtgttatgt aaccttctaa aaatccttcg gttcttgctc
     2821 cccaagatgg atcaaagtgt atgacgccat ctgctcccca agcttcacaa gattttatat
     2881 gcgcaatgtc cataaacggg cagcctgttc cgtattgtat gaatactaca gggtttttat
     2941 ccgtatgctg gagcttcatt ggcctcattc gagaattaac atcaccaacg tatttactca
     3001 gtgcacttac ttcatcactc gagagtggtc gggttttttc acgatactta ccggcttcat
     3061 aaaaggattt cactacatcg tcgcataatt tagcatattc cctgcgaaat ttatcttttt
     3121 ctgattcact ggtgctctct attatctgcc ggcgaagctc agccctcata attgccgtag
     3181 tagttttatc acttacatag tcaagaattc catcgactat agtatcgagt agtttttcta
     3241 ttcttttatt gttgaaatcg tacttgtaat ctgctgtaat atccttcttt tcctgcttaa
     3301 ccccggtttc tgcaggtata tattctctat cttcaacaaa agctacagct tcctccacag
     3361 ttgttcccgg accaaagcct gcatcaaaac ccagctctgc agcaagttcc ggtcgtattg
     3421 ccattccacc tatgcctatt tttaccctat ctcttaagcc agcagcttca gccaggtcga
     3481 taaatttgcc caagatttct gctacaccgt aacctaaagt acggctgatt aaaacaacat
     3541 ctgccttgtt gtcaattata gctttaataa tctcctcctg cgtatggtct ggaggcatga
     3601 gtatagtatc atggccattt tgttccaggc cccgctttat catttttagc ccaatatcgt
     3661 gaactgggtc caagggtgct aataaaactt tctttctgct catgacatac tcctccctaa
     3721 ttgctttgat caaagagcca gctatatcta tcgccgggct tatagatgta tcctctggcc
     3781 cgaattctcg cgccaggacc ttgatgctgg atgaagacta tagttgtatc tttaaagcct
     3841 agtttttcca ttaattccaa tgtaaatttc tctgcttcct cttcgtcttt gcaaaccaaa
     3901 tatgtctcca ggtcaacggc gtccattact ttttccaaga tgtccgacat gctatctcct
     3961 cctatactca aatagtccag aatatcatac acttaatcat tatctgaact gctgtcatct
     4021 accccattga ctatttccca gtatctctta acatctcttt ctagcttctc tatatgatcg
     4081 atcattgctt tttctgccaa ttcgggattt cttgaagcga tagcttcaaa aatctttttg
     4141 tggtctaaaa ggaccgtact tttgacttct tttcttataa actcaagcat tggtgacagt
     4201 tgaccgtgct gtctgattaa atccattgct gcatccaata ctctatttcg ggcggctttg
     4261 gaaattatct tgtgaaactc cacatcatca tcagctatgg aaataccttc tttgacatgc
     4321 atctgctgtc ttttaacaat gttttgcatt tcgctgattt cttcatcagt tatatacatt
     4381 gctgccaact ttgccaattg gctctcaatg gcttttctcg ctattaataa atcaagcaat
     4441 tcttgtttgc cggttacctt tataacattg agcaattcct tgccgtaatg attgattgta
     4501 ttctcacgtt ccagttccct tagctttttt ttgcccgctg gagtcaactt tctaccctta
     4561 aaaccaattt tttccgtata accctttata tctaactgcc tcaatattct accaacagtg
     4621 gcttcactaa tatttatacc ctgcatttgt aaaatgttgc gtacgtaacc tgagcccaca
     4681 ggaacttcag aattatctat ggattttaaa attaaaaata acatccgcat tttatcatgt
     4741 tgcatgtcaa acccccattt taatacttac ataattaact atacataaat tttctagtac
     4801 agcaccggtt aaaactataa atttaatatt atggcatatc attaaacttt taacaaaatg
     4861 attgaaaata aaaggagtca gtgatgaccc cttttatttt cgcatttgat gcataatatg
     4921 ttatacaact ttctgattaa acacgtcagt cattcctgct ttttctacac gttcctttat
     4981 tgcatttaca tcggtagaat acaaacctaa ttctttcatt ctctcaaagt atacggagcc
     5041 agaaaaggtg tatttcttag atataccttc ttcagtcttc atctgttcct ttacataagc
     5101 aatagcatcg cctactgcaa gttctgttgg aatctgtaca agttcttttc caactgccca
     5161 ccttacggca ttggctccag caagagaacc ggtgcaaatg gcttcagtat ggcctaccag
     5221 aggtcctgct ttttcgccag cgcaaaatag gttttccaac ccttccgtct ttagatgatt
     5281 atctctaggt gaaagagcaa gatatcttat ggagttgccc aggtttcctg cataaggatc
     5341 ttcatacctg gcattctcaa atccggggat ttgtcttaaa tcttctaagg ggaagaaggg
     5401 actcatcaat tttgcatgtc cagtatcaag tagtattaca ttgtccacgt actctttcaa
     5461 agcgtactgt tgacaggctt ttttagtaag gatatctgat ccttcttttg aatgctccgg
     5521 cacgggtata actgccacac cagttgaatt taacttgtct acaatttcct gtgaaagtga
     5581 ttctttaaag agtttacaag agccgctcat agctccataa ctgccatctg ctttcttagc
     5641 catcatttct ttgacgccca tcttggccgc aatgctcact cggggtccat agcttgggca
     5701 cctgtatacg cacattgcgc atccatttcc gtacttggta caattgcttt gaggaccagc
     5761 agttccggta cactcaacaa aaacatcgcc ttcaaatttt ctgtcatctt ctgtgatcac
     5821 agctaatatc ttgttgccct cgccaactac atccttcaca cggctttccg tatatacttc
     5881 aacacctgca tctatgagga cttttttgac tgccggttcc actaaagcca catcgtacaa
     5941 tgttgcatgc ttgtgaccag gaaactcaat gtttttatgt ctcgctacac tatcggtaac
     6001 ttggaacaat tcgccgccac ccatagctat catttcttct gtagcagtaa accttccgtt
     6061 atttctcatg ataccgccaa caaggcctgt tcccaataac atgtcagttc tttctagcaa
     6121 gactacatct gcaccggctt ttttggctga taaggcagct gcagtgccgg cccagcctcc
     6181 tcctataaca actactttgg gcatgcaaat acctcctttg atttttcagg ctgacgtcaa
     6241 ggctttgcct tgacgtcata caatttctaa ttaatcaata tatccactgt acttggcaat
     6301 gatctccatg agcttgacaa gggcttttac tgcatatctg aacttactat caatgatctg
     6361 tccctcatca tccgtagtac caaggcccgg tgaaatcaga atgtttccgt cataagcaac
     6421 tgttggaatg acacccatct gtgagaggcc tgtttgcaat aactggcttc cagcatacat
     6481 gtcttcgatg gagaagattg ggaatcctaa ttcgtcgcct tcccaggtct tttcgtagtt
     6541 aacttcaacg gctctgctgt tgtaggattt ggatatgagt atgcctttct taagtttagg
     6601 atgaatttgt tcaaattctt ctttaacaat atcgtacatg tcgtcaacgg gtttctttaa
     6661 gttttcagga ttatctctca attctttcag cgctgcaatc attccgagca ttgcttcttt
     6721 acctggatca aatccaacat aagctgcttt tccataggat gatgtggtgc cgtagcggtc
     6781 accgtgcatt cctagggctc tgcgaattgg taccataaat tcttctttac ctatgattaa
     6841 acctgacgta ggtgacccgc tggccttatc catactataa attattacgt ctgcaccgct
     6901 cttggtaata tcatgaccaa tgaaaggaac tccccaagca ttatccaaaa catagggcac
     6961 gtcaaagtct ttggcaattt ccgccaaacg cttctgcagt acaggtacac catcctcgtc
     7021 ttttacgcca tagccgtatc ccggtgtgtc gtaggcaagg gatgtgatac cagtgagcat
     7081 cgtagcatgg cgttcggcaa ctttctttat tttttcagct gaagcttcgg gatcgacatt
     7141 ggtaagctgg aaagtggggt ggaacttaat accgtgtacc gaataatcgg ctcctaccaa
     7201 tggtactata tacatactta agttattttg tctcttgcca tagaaaccta gttcgccggc
     7261 ggttgaacct ctgtctgcaa gaacgtcttt aaatctgccg gggaagggtc ttccgtaacc
     7321 accgtggtgg tgtaggtgtt tttcataggg aattatataa cgggcctgat aattgtcacc
     7381 gcgcccctgc attggtggtg aaaatagaac gtcaaggcaa cccatattcc tgcttcacag
     7441 gtgctaattg gtgcagcatc atattcatca ccataaacat ctttaactat tgatctgatt
     7501 tcgtctacta acttagataa aggaattact tcctgagcac ctttctcgac agcttctctt
     7561 atatttctgc tcaagggaga aggacagccg gaaatagcac cggtcaaacc aaacttacct
     7621 cttaaatctt caggaattcc tatctcatct gcggcttttt tcgcttccgc atagatattt
     7681 gcaatgtttg cttgtaaata ctgatacatt tgaaactttt tgttaaactt tgccattgta
     7741 tttcctcctt gttattttaa ttttactatt aattcaactt cacaagtggc gtccatagga
     7801 agctgattga caccaacagc cgagcgtgca tgggcacctg cttcaccaaa tatctggcca
     7861 agcagctctg aagctccatt gataacctga ggctgctgat taaaaccatc ggcactgttt
     7921 acaaagccaa caaccttcac tattttttca acattatcta aacttccagc taaagacttt
     7981 actatgctga gacagtttaa tgcacatatc ttggctgctt catacgcgtc ttctacggaa
     8041 acctcatcac cgactttacc tttatacttt aattgtccat ccacaaaggg aatttgacct
     8101 gcagtataaa tatatttgtc tacttttaca ccgggaacat atgcagctac cggttttggt
     8161 ggttcaggta acgcaagacc taattcttga agttttttct cgtacgacat acgcacaaca
     8221 cctcctttca agtagtgaaa ataaaaacca ttaaccaatt actacctcat catcaaggtc
     8281 aagatttctt tcattgcctg aagtgcgttc ttcaccgtat ataacgatat tgtagtgatt
     8341 tggcatatga gtttcatcaa agcctgcaat gtttccaatt acttgtccgt ttattttaac
     8401 ttcatcgcct actgttatta ctgtgctttc ccttacaaca aaaaaaccta tataagcaat
     8461 tttgtttact acatcccctg gcttcttagc ttcatcagtt aaaatcagct catgaacttc
     8521 atcagcaact agacaccttg atggttgtgg gataagctgc agaccccgct cttcatactt
     8581 gccgtctaat acaacgacta agtttccttt taaagctttc ttcttagcgt agggattggt
     8641 tttaaacata ccggttttat atggatcagc cataaatcag cctcctcatc aatgatgagc
     8701 tattttatta tattacttcg acaaatataa cgaaaatcct tctcataaat taactttttt
     8761 aacaaatttt tttcttagat atttgtttcc catttcatcc aaaatctaaa tattctatta
     8821 actaggtcat agtattttct taatctgaca tcaccggcag cgactaagtt aactgattcg
     8881 acgaaactat tatctatata aatctgcaac tggcctatgc tttccccctt aaaaactggc
     8941 gcacttattt ttttaggtgc aattagataa tagtttactt tttcgccctc ctttatcggc
     9001 aacaggatgt tatcttccgc aattaaacct acgcttttaa taacaccttt ttcaacagtt
     9061 acgcttgtac aaatctcatt ttttctaact ataggtttca gataataatt ttcaaatcca
     9121 taatctaata atctttgaga atcctgaaag tggtcagttg aacctaaaac aacggatatc
     9181 aaacgaaatc cctttttgtt tgcagtagcc actaagcatt tgcccgcatt tcccgtataa
     9241 ccggtcttta tgccatcagc tccatcaaat tgccataata gccggttggt gctggatatt
     9301 tgtctggcaa aatttccatc ttttatttct tttgttttag tttgaacaat ttcacgaaat
     9361 ttattatact tccatgcgtg ttttgcaata actgctaagt ctttagcagt ggtaaaatgt
     9421 tcagggtcat cgagaccatg gggatttgta aagtgagtat ttaaagctcc taattccaga
     9481 gcttttttat tcatcatttg cacaaatttt tcctcagtcc ccccaatata ttctgcaatc
     9541 gccactgccg catcattgcc cgatggcaat aaaagtccat acagcatgtt ttctagagtc
     9601 atagtttctc cggatttgag ataaaaggaa gaaccgccca tagaagcagc tttttggctc
     9661 acttttattc gggaatttaa gtctcccttt tctaaagcta ttactgcagt catgattttt
     9721 gtggtgctgg ccataggcat tttttcctgt gcattttttt ccaataatat tctaccactt
     9781 acagcatcca ttaaaacaaa agctttcgca cttaatggca atgtttcatt ttctgctttt
     9841 gaacaagcag agtaacctgt tgcaaaataa caaaaaataa acaagacaac tgccagtttt
     9901 ttagtcatca tttgccctcc caatatattc ttttattaat cggccaagtt aattcttgta
     9961 aaaaatggag gtcttgccaa aaatactaat agtcttgaat aaatgatatc ccagaatagt
    10021 atttcacaca ataaaaaaac ggccgcattt catggaccgt cataaaatca ttgatgtttt
    10081 ttcagcagca tatttatttg gtcaataatt tgcggagcaa tgtctatcat tctttctact
    10141 gttgtgtttt gttttaccga caatagtcta atttgcccct gacctactac catgaatgcc
    10201 accggttgaa ccgatactcc ggcaccgctg ccacctccaa aaggcagctt gggtttttcg
    10261 cttttatcat tatcttttga agccttaaca tcaaattcac tgccacctgc cgcaaatcca
    10321 aaacaaaccc gagaaaccgg aatgattacg ctcccatcct gagtctccac agcatctcct
    10381 actatcgtat tgacatccac catctctttg atactttcca tagatgtctt aatcaaactt
    10441 tgaataggat gttcgtccat cggcaagaca ctccttacta tgtgaatgtt ctcagctgtg
    10501 ttgatgtttt ttcgtcagag accttatctt atatatcatc aaataccagg ctactataaa
    10561 acttgcaatt ataatatagc caactctaag actaaatatg cagtcgattt tcatttgaaa
    10621 agatttgtgt ttaaaaaaat caggtgttac cacgaccttc acttttgcac cttcaaaatt
    10681 taaccacacg gatagtaagc ctaagctttg agaaattatg gtccaaacag tacctgccaa
    10741 aactcctgta aaagccggat tttcgctgcc gaaagcaatg tttatattta ggtaaacgag
    10801 atttactttt ttaaggagtc taataattag tattttaaaa gtataaaagt atgtaacaaa
    10861 gaatttcatg ctatcaatta tcttcctaaa aatgtctcta agtggtgaaa tgactatttt
    10921 cccagattta gcttttgcgg tacttttact gccaacttta tactcaaaac taagaattgg
    10981 aacaaagctt ttcacagtta atttcatctc gggtatctct aaaaacactt ttgacatgta
    11041 cgagtccatt tcaaacttta tatattcatc ttgtattttt ttaattaaaa caatactaaa
    11101 tctcacctgt aaaaaaaaga aaagggaaaa agctaggatt aaaagtaaat atatcacttt
    11161 aatctcctta aaccttttcc cttagtttgt ggaaaaaact tgttatcgat tcattttatt
    11221 caatggaaat ctcatcaatg ctctttagac caaagtaatc aagacactct tcagttgtaa
    11281 cataaagtat aggtctgccc ggcacgctta agcgcccctt ttcgcaaatt agattttttt
    11341 ctaaaagagt gcttatggct ttttcgcaat ttacccccct gattgattca atctcggatt
    11401 ttgttatggg ttgtttaaac atgataatgg ccagggtttc taatgcagct tttgaaagct
    11461 gcgatctaac tttcggttta aatattcttt caatatatgg aaagatttcc gctttcgtcg
    11521 taagcctaat tcgatttccc actattgaaa tcattattcc gtgacaaggc ttgttatact
    11581 ctatttctaa ttgttttaca cactctagca cctgtttatt tgtaatattt agtgcatgtg
    11641 caatttcatc aatgcttagg ggattgcccg atgtaaacag tattccttct ataatacctg
    11701 taatcctttc taattccact ttgcacacta aacctcccta caggttatga cgatttctcc
    11761 gaattgcttt tcttggacgg cgttgatccg tttcattttt accagctcca atactgctaa
    11821 aaaggtagtt attatttcga ttttaggtgt ccgcttgtca tataatctac taaaaaatac
    11881 ctttgacttt tgcctcagca ttttgtaaat ttcaatcact ttacgaaata ggggcaatgg
    11941 atcttttgaa accttagcaa ttctttcctc aactttctct tggctcataa cacgtttaaa
    12001 tgctaattta atgtcttcaa aacttatatc agccagttca atttcatttg cccacattga
    12061 ggataattct tcgggaggtt ttttaaagag caactcctgt tcttgctctt ttttctttag
    12121 caccatagaa atctctttaa actttttata ttcaagtagt ttgtacatca gttcacttct
    12181 cggatcggca gtatcatctt ctgccgctgc caattcactg accacttcat gttttggctt
    12241 gggtaataac atcttggatt tgatactcag cagagttgct gccattacta agaattcact
    12301 ggcagtatct aatcggcttt ccttaatttc ttgaatatat ttcaagtatt gctcggtaag
    12361 ttctgcaata ggtatgtcat aaatatcaat ctggtttttc tcaattagat gaaataaaag
    12421 gtcaaaaggc ccttcaaaag catctatctt tacatttaaa gccacggctc ttcaccttta
    12481 attatatttt cattgcctct ctagcctctt ccaaagttct ctctgcaact atgcgagcgc
    12541 gttttgcacc gatgtcaagt atattatcga tgatagaagg attttgttcc aattcatggc
    12601 gtttttggtg aattggttcc atcttgtcaa ttattattga tgccagcttt ttcttgcatt
    12661 ctacgcagcc aattagtcct ttttcgcata tctccttgag ttctgctacg ttgtcttttt
    12721 ggtcaaaaac cttataaaaa gcaaagacat tgcagatttc cggatggccg gggtcatttt
    12781 ttctaatctt tgctgggtcg gtaatcatca ttcttacttt ttccattatc gttttggggc
    12841 tggcagagat ttcaatatgg ttgtcatagc ttttgctcat tttcctgcca tctattcccg
    12901 gtaaaaccat gaattctgta taaagggctt cgggttccgg aaaaactggt ttataaagat
    12961 agttaaatct cctagcaatt tctctggtaa gctcaatgtg cggttgctgg tctttgccca
    13021 ccggcaccct atctgcttta tatatcagta tatccgttgc cattagaacc ggataaccca
    13081 gaaaaccgta tgtgttgatt tctttgtttt ctaattgttt taactgctct ttgtaagttg
    13141 gacacctctc caaccaagac aggggtgtta tcattgaaaa taaaagatgt aattcagcat
    13201 gctgttttac attggattgt acgaaaatat tgcatttttc ggggtcaagg ccaacactca
    13261 cccaatcgat taccatctgc cgaatattct ctttaatttc ttcagtgctg ctatattcag
    13321 tagtaagcgc atgccaatcc actactacga aataacaatc gtaatcgtct tgcaatttga
    13381 cccaatttgt caatgctccc atcatatttc ccatatgcag gcgacccgta ggtctcattc
    13441 cgctcatgat tacatgtttt ttcacagcga ttcctcccat aattaaaatc ttaataaagc
    13501 caaattagat aaaacatcaa ggcctctatg gataaatatt attaaaggat ttaagatttt
    13561 gcccaataaa ccgaagtaaa ctagcaatat tagtacaaaa ggtccataag cctcaagttt
    13621 tgaaaactca tatgcctgtc ttgcaggtaa taggccggct agaatctttg atccatctaa
    13681 tggcgggatt ggtatcagat taaaaacagc taaggtcaag ttgtaaagaa acagtatata
    13741 aataaaacta tccaatacgc ctatgcgaag aatgtcaagc ttcaaaatga tcatcgcaat
    13801 aaacgccagt atcaaattgg ccatgggccc ggccaaggaa accaatattg tacctttttt
    13861 tggctcttta tagtaactcg ggtttatctc aacgggttta gcccaaccga acctaaatag
    13921 ccataacatt attaatccaa tcgggtctag atgaacgagt ggatttaggg aaagtctacc
    13981 cctccactta ggtgtcgggt cacccaagga gtaagatact ctggcgtggg catattcatg
    14041 aaagctaatg gctatcaata gagccggtat tctcaacagc atttcaggac ctatataatg
    14101 tgtcatgtaa catgaccatc ctttccatca tttagtatta ttaaggtcta cttattgcat
    14161 tataccattt aaatcaaaat gaaaaaagtg cgcaaaagca cacttttttc aggacaacta
    14221 aagatatttt acatgcttgc agtatagact ttctttgcag ggggcaaata tatctcattg
    14281 cttagtaggt cctcgtatgt ctgtcgacga acaacaagcc gggctgtatt gtttttaaca
    14341 aataccacag ccggtttggg cagcatattg taattgctgg ccatcgaata atgatatgct
    14401 cccgtgcaga aaactgccaa tatatctccc gggtcaattt ttggcaggtc gatatcccaa
    14461 atcaacatat cccctgactc gcaacatttt ccagcaatag acactctttc ttccttggga
    14521 cttgccgctt tattcgcaat taatgcagta tatttggcac catatagtgc cggcctaata
    14581 ttgtcgctca ttcctccatc aacactgaca tattttctta tacctggtat attttttatt
    14641 gcacccactg tatatagggt tgtcccagct tcgccaatta ttgctcttcc aggctcaatt
    14701 gagatcttcg gaaggggaac atcataattc tggcaacatt tcttaactga tgtaaccaat
    14761 gcatgaatat actcttttac cgggagtggc ctgtctgttt caagatattt aattccaaaa
    14821 ccaccaccaa aatcaatttc tctggtgttg aatccatatt tatcatttac cgtctttgca
    14881 aatttaacca ttatttgagc agcatcttta aatggctcct cttcaaatat ttgagaacct
    14941 atgtggcagt ggaaacccat tagctttaaa cctttaatac ttaaagcact ttccacagcc
    15001 aacagcgctt gtccgttttc cattccaaac ccaaattttg aatctagctg ccccgtcttt
    15061 atataatcat gcgtatgagc ttcaatgccc ggggtgagac gtaaaagtac ttttatgttc
    15121 ttagcagggt attttttgct taaatcactt atcaattcaa gttcataaaa attgtcaact
    15181 acaattcgcc cgacaccgta ttctatagcc atttttaatt catcgtagga tttattatta
    15241 ccatgaaaat atattctctt tgcaggaaac tgagctttta tggcagtgta tagctctcca
    15301 cctgaaacca catcgaggca aaggccttcc tcttcgataa ttcggcacat ttctatagtt
    15361 aaaaaggctt ttccagcata aataacgtca gtgttttcat gtaaactgcg tatactgttc
    15421 aaatatgtgc gacagttttc ccgaatgaga ttttcatcca tgacatataa aggtgtgccg
    15481 aattcttttg ccagctctac tgcatcgcat ccacctattt ctaaatggtt aagtttgtta
    15541 actctgcacg ttcctaaaag catattgctt ctcctcccat taaaaacaaa aacaaccggg
    15601 atatttgccg gttgtacttc ttcaaagggc aaatacctca ctcatctgaa gtagcgctcc
    15661 accggtgata ttaccggtga cagtcttata cctattcggt ataagcccag catggttttt
    15721 ggggaaaaac catacttcgg cgatgacccc tttcaatctg cttaataggc ttcccctgcc
    15781 tccacaaatc tactgatggt cgatcgcgcc tctaccccaa ctcttatcga gatgaggtaa
    15841 tgctattgac tttttcaata caataacaca tagatttgct attgtcaagg tcattctgaa
    15901 gggatttgga aattatattc tgaaaaaaag tcagtaatgt tgcaacatag a
//
LOCUS       DAONUO010000005        15441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_2860_length_15441_cov_3.842194, whole genome shotgun sequence.
ACCESSION   DAONUO010000005 DAONUO010000000
VERSION     DAONUO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 15441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15441
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2860_length_15441_cov_3.842194"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PK768_00410"
     CDS             complement(<1..439)
                     /locus_tag="PK768_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HQA59468.1"
                     /translation="MYKKIFLATLMLLLILSIPICAMASPKPIEIYINGEKVESDVAP
                     IIVNDRTLAPVRVISENLGAEVYWDNDNRLVQVETPSKTIILKIDDTRALVDGQEVVL
                     EVPAKIVNDRTMVPLRFLGETLGAEVSWDNDLRRVIINRSGSKV"
     gene            complement(584..1546)
                     /locus_tag="PK768_00415"
     CDS             complement(584..1546)
                     /locus_tag="PK768_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HQA59469.1"
                     /translation="MRRLLHAVLSLFIVFILIGCSAQSANDNEDSAYEDIIESGKKVM
                     VYYIKDGFLTPVTYSIDDSELEINAAVNLLFSGILPDGFDSCLLSTGLNTMKISGDTV
                     SLDISGNPFQGERAELARKQVVLTLTDCPNIINVNISINGQPYGDSLKRPSHINLIDP
                     DTYQQDQANPDELDKYITIYYPDKTAKYLVPITIKSDKIDGSSASSVKASELAKAALQ
                     HLIEGAEEIGLGFNDKMIKSLQISEGVAVVDLDRNILLNKFSTKIQYAEIAIKSVVRT
                     LTSIDGIEKVQFLVDGVKMGYITGNINIQNPIKPDKWYNFLTAA"
     gene            complement(1578..2300)
                     /locus_tag="PK768_00420"
     CDS             complement(1578..2300)
                     /locus_tag="PK768_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HQA59470.1"
                     /translation="MEQPGEIKKVSMAVIKRLPKYYKCLGELLRKNVERVSSQELSKL
                     LGFTASQIRQDLNNFGEFGQQGYGYNVSQLYNEVAKILGLTTSHKMVLIGAGNLGQAL
                     ANYSGFATHGFNIIAIFDTNPSIVGRKIRDIPIYHVDDMEYFINSNDVDIAVLAVPGD
                     VTEKIVEVLVNTDIRGIWNFTPTEINVSNKDITVENVHLIDSLLTLSYRINESWLRER
                     LLKQEGKNTKFPHRKKMSFMVK"
     gene            2456..3940
                     /locus_tag="PK768_00425"
     CDS             2456..3940
                     /locus_tag="PK768_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQA59471.1"
                     /translation="MGHWLVNTVLLTATNTITGTIGFFYRLVLSKYLGSEGMGVYQQT
                     LAFFGTAITLVTAGIPLSVSKLIAEMQTEANKRNHIILSAFTLTTAFSLLGASFLISL
                     SFLLKLKLLLIVLPAAIFVGYSSVMRGYFFGMQDTRPVQWSNITECIFRTLFGVLFVS
                     SNLLIGFETKTRGAVTALTLGEFISMCILFIFFAKSIGNYRNSRKNSVIATIKDILKI
                     AVPVSFSQIIHSISRSIEALLVPKGLVLSGLSSYEGLALYGKTSGMVMPLIFFPALFV
                     RALSSNIIPQIAKALALKNKEYAFKLSQQALFLTSFFAFAVTAFFIALSEPVAEFLFP
                     KFELGNLIIAFSAGIPFYYLESIIMSILRAIGNNSTPITTSLTTLFITNQILFLLTAK
                     TPMGIYGYSVALITASAVAIFISIRHIEQNFQRQFNLLEIILKPMICCVFMAYIMSKA
                     FLPLVNLDFPKIICIGADLILGISGYLILALALGIDLRGIRQRY"
     gene            4022..4414
                     /locus_tag="PK768_00430"
     CDS             4022..4414
                     /locus_tag="PK768_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59472.1"
                     /translation="MKTIRLFHRLSSGQYLAMYVTRHRQRSKSVAYIVAVCIFPTKRE
                     CNYWFRHQEELISKSFNTWGLEGMLKALKWLKQLKHAIGPGESIVIYWVDERRRRAFK
                     FLERYGFKQGEYLGRPCYIFKNSGSSLG"
     gene            complement(4463..5548)
                     /gene="ychF"
                     /locus_tag="PK768_00435"
     CDS             complement(4463..5548)
                     /gene="ychF"
                     /locus_tag="PK768_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQA59473.1"
                     /translation="MKVGLVGLPTVGKTTFFNLLTHAKAETNAFQSGKQNANIGLARV
                     PDGRIDFLAQVYKPKKVTYAQIEVVDIPGLTRGAGEGKGSGNQFLSNIRQVDCLVHIV
                     RAFRDENVIHSEGSIDIIRDIENIGTELLLADLQLVETRIDRINNGRKVTKEQVAERE
                     VLEKIRESLENEMSLADFNLSEDEEKLLEHLDFLTAKPVIIVVNVDDEQLIEGYEQKD
                     EVLRYCHEKNLPVFEVSAKAEVEINELDDDDRIAFMRELNIKESGIDLLARAIYDRLS
                     LISFLTVGEDEVRAWTIKKGTVAKKAAGKIHSDIERGFIRAEVISFDEFKECGSMAKA
                     RDLGKLRLEGKDYVVQDGDIISFRFNV"
     gene            complement(5745..5820)
                     /locus_tag="PK768_00440"
     tRNA            complement(5745..5820)
                     /locus_tag="PK768_00440"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5785..5787),aa:Ala,seq:ggc)
     gene            complement(5905..5979)
                     /locus_tag="PK768_00445"
     tRNA            complement(5905..5979)
                     /locus_tag="PK768_00445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5945..5947),aa:Val,seq:cac)
     gene            complement(5984..6059)
                     /locus_tag="PK768_00450"
     tRNA            complement(5984..6059)
                     /locus_tag="PK768_00450"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6024..6026),aa:Ala,seq:cgc)
     gene            complement(6124..6648)
                     /gene="lepB"
                     /locus_tag="PK768_00455"
     CDS             complement(6124..6648)
                     /gene="lepB"
                     /locus_tag="PK768_00455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQA59474.1"
                     /translation="MSNDIKREIFEWLKAIVLALILAFIIRGFIFEPMIVPSGSMIPT
                     IQINDRILVNKYIYRFQSPEYNDIVVFRYPDDHKQIFVKRLIGKGGDVIEIRDSVLYR
                     NDEPIQEPQLNEAMYNDFGPYKVPEGHYFMLGDNRNNSKDSRFWDNKYVSREQIVGKA
                     TYRIWPINRIGALK"
     gene            complement(6718..7458)
                     /locus_tag="PK768_00460"
     CDS             complement(6718..7458)
                     /locus_tag="PK768_00460"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQA59475.1"
                     /translation="MERVKLKVPGKSTFVLATLLILALIIQYLYGKNLAVSVLSGWLP
                     LSGKVIVIDPGHGGIDGGASFDSVLEKHINLEVSLKLKSILEKEGANVVMTRTKDIAL
                     DHLNNKSEYRHKRDLIARVDIINNSRPDIFLSLHVNAEKSSAKTRGPMVFYYYDSVKS
                     RQLALQIQKNLEEAYIKAGHRVPARKANGNRSLFILKNTKPAGVIVELGFITNSGDRQ
                     LLTTEAFQKQIAKAMALAIKEYFSQKAL"
     gene            complement(7829..8983)
                     /locus_tag="PK768_00465"
     CDS             complement(7829..8983)
                     /locus_tag="PK768_00465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQA59476.1"
                     /translation="MNNKKGKLWPYLVALALIIFAGVMLVYRDAFNDYVSVLATWQPV
                     TTSKNADSDDVNTEEELSQEEPEEVDLPNPTATFLAVGDVMMHDGQIWSGYSDAADSF
                     DYTEFFHVVKDEISAADIAMANLETTLAGKEQKYTGYPMFNSPDEIADALKDAGFDII
                     VTSNNHSLDRGAKGAIRTIQVLKERGLAPIGTYESEEEREQILIKELNGISFAFLSYT
                     YGTNGIPIPKDMPYLVNLIDEELILSDIEKARSQADVVVVYLHYGDEYQRTPSKYQRK
                     LAHLLLKNGASFIVASHPHVIQPGEWMEVQEADGEIIKKYIAFSMGNFISAQRFPYTE
                     EGLMVKFTLEKDLKKNKVYLTDVEEIDTWVDKFVRDGKMRYVVRFGKKPT"
     gene            complement(8983..10065)
                     /locus_tag="PK768_00470"
     CDS             complement(8983..10065)
                     /locus_tag="PK768_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HQA59477.1"
                     /translation="MNIYGVIMAGGGGTRFWPLSRATAPKQFLNITGEDSMINDTIKR
                     IKDVIPPENILIVTNKAQEKILNEVIQEDIPKENVLVEPVGRNTAACIAYAAIVIKKR
                     YGDSVMAVFPSDHYIKNVDEFQRVLRSACSIAESTEKLVTMGIEPTYPSTGYGYIKYD
                     KDRSLPAGNKTAYEAVEFVEKPSVPKAKKYLESGNYLWNSGMFVWKSSVILNNFRRFL
                     PRLYRNIEKIEPFLGTPEEGKAVEDIYPILPSISIDYGVMERSDEVVVIPGDFGWNDV
                     GCWDSLGSVFPPDENGNITKGDFVDIETRNSIIYSNSRFVAAVGLENMIVVETSDALL
                     VCSKEKAQDVKKVVEYLNKVGREELL"
     gene            complement(10227..11108)
                     /locus_tag="PK768_00475"
     CDS             complement(10227..11108)
                     /locus_tag="PK768_00475"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQA59478.1"
                     /translation="MTLCKKDTLIGFIGTGVMGKSMAMNLMKAGYKVLVYNRTKAKAE
                     ELIEKGATWKDTVAEVAREANVIITIVGYPKDVREVYLGENGIIENAKAGSYLIDMTT
                     SSPKLAKEIYEAAKARNLHALDAPVSGGDVGAKEARLSIMVGGDAEAFEEVKPIFEVM
                     GKNIVLQGGAGAGQYTKMSNQIAIASNMLGVCEAMAYAIKAGLDPKVVLKSIESGAAG
                     SWSLSNYTPRMLSGDFEPGFYIKHFIKDMKIALESAKELGLKTPGLELALSIYEELAE
                     RGEENSGTQALFKYYMN"
     gene            complement(11145..11666)
                     /locus_tag="PK768_00480"
     CDS             complement(11145..11666)
                     /locus_tag="PK768_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HQA59479.1"
                     /translation="MQRIDVILGDITKVEVDAIVNAANKTLLGGGGVDGAIHRAAGPE
                     LLEECRQLKGCETGQAKITKGYRLPAKHVIHTVGPIWQGGDANEDELLASCYRSSLML
                     AVENNVKTIAFPSISTGAYGFPVKRAADIAIREIARFLEENNSIEKVLMVCFDEKTMK
                     AYSEALSKLRKEY"
     gene            complement(11752..12672)
                     /locus_tag="PK768_00485"
     CDS             complement(11752..12672)
                     /locus_tag="PK768_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HQA59480.1"
                     /translation="MASSFSRLIQGVWRLADPKLCIASTVPMVVSTAMAYKALGYIDI
                     YWLFLSFFGVYLIETGKNAINEVVDYKSGVDRFVTPDKRTPFSGGKKTIVDGLLTVEE
                     TKIIALVTMLAAAAIGLYIAIAREPMVLWIGIAGFLISIFYSLPPFKFCYRGLGEFAI
                     GLTYGPLVVSGTYLVQTHTVNLEILLLSLMIGFLIANVVWINQYPDYEADARGNKRNW
                     VVRLGKEKGIVVYILLYVLAYLSLLIAVFFTGNPLLLIALLSAPIAKRSVDVAKKYYD
                     DIARLVEANAKTIQVYQMTGLTLTAAILIG"
     gene            complement(12802..13773)
                     /locus_tag="PK768_00490"
     CDS             complement(12802..13773)
                     /locus_tag="PK768_00490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HQA59481.1"
                     /translation="METQKELNTLLNKNYKLLALDIDGTLITSGHLVTPRTLKAIKKV
                     KDLGIRVTIATGRHYHSVRRLARIIGVNAPMVCCDGAIITDKDSSCVTYHLLPHEIAV
                     DVMRMTLDYEGFKVLLFVKSGKIYGGRRYQGQYFKRFFRVPLKYTFKGYLNLLRDFAF
                     MPIKNMGSTQTVISALEEPVAKVFIYSNGRPGDLKDFINKITAKYGEEISITSSIPNC
                     IDVLKGGVSKAKGLIELAETLGISHDEIITVGDNYNDIEMLKYAGLGVAMGNAPELVK
                     SKADYVTDSNDDEGLAKFLEKLISHRDGASCFQLANQSSVAAAGKLH"
     gene            complement(13873..15105)
                     /locus_tag="PK768_00495"
     CDS             complement(13873..15105)
                     /locus_tag="PK768_00495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQA59482.1"
                     /translation="MLPILHVDMNAFYASCHQAKDPSLKGKPILVAGDPKKRTGIILT
                     ASYEARKYGVKTAMPNWQARKLCPQAIFVEPDYELYVRTSQKVMEILGRFTPLVEVFS
                     IDEAWLDVTGCERLFGDSVTIAHKIQAAIRSELDLLCSIGVSSNKLLAKMASDLKKPD
                     AVTVLPPEDVPKLIWPLPVDELFGVGRRMAEHLSHMNIKTIGDLARVPQGLLGKAFGL
                     NGRYLHLWANGIDTSPVDPHAMDEAKSMGHSVTLPKDITSYDEAETVLLSLSEQVGRR
                     VRRENYMGRTVTITLRDNSFCTITRSATISYTNSTEDIYNLAKKLLYSNWDGKTPLRL
                     LGVSLSQLVKEFEQVSLFDTDEKKKKLNKVMDEIKDRFGDGVIFRAKLLNDEKLNRRK
                     LGKSDNNAPTLKGIKTMK"
     gene            complement(15259..>15441)
                     /locus_tag="PK768_00500"
     CDS             complement(15259..>15441)
                     /locus_tag="PK768_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59483.1"
                     /translation="ALGTWQDAKLPVNPCGVKGQALEQQVLILGDYRYFTGYHMTSKI
                     TLSCRYHNLIARHTMV"
BASE COUNT         4120 a         3669 c         2846 g         4806 t
ORIGIN      
        1 cgaccttgga cccggaacgg tttattatga cgcgccgcag atcattgtcc cagctcacct
       61 cggctccaag ggtctcacct aaaaagcgca gcgggaccat ggtcctatca tttacgatct
      121 ttgcaggcac ttccaggaca acttcctgac cgtctaccaa tgccctcgtg tcatcgattt
      181 ttaggataat tgtctttgac ggagtttcta cctggaccag cctgttgtca ttgtcccaat
      241 aaacttcagc cccaagattt tcagaaatca cgcgcaccgg agccaaagtt ctgtcgttta
      301 cgattatagg ggccacatcg gactcaacct tttcgccatt tatgtaaatc tctatcggct
      361 tgggagaagc catggcgcaa atgggtatgc tgaggatcaa taaaagcata agtgttgcca
      421 gaaatatttt tttatacatt gtaacaaaac ctcctatgat tggtgcagcc ttgattcatt
      481 tttatgtact tcgactttat ctttcaaaat cctttagaaa aggacaagtg taatagaatt
      541 gtaaagttga aaaggaaagc tttggacaaa aaaataaagg ctgctatgca gccgtcaaaa
      601 agttatacca tttatcaggc tttattggat tttgaatgtt tatgttgcct gttatatacc
      661 ccatctttac gccatctact aaaaactgga ctttctcgat tccatctatc gaagtcagtg
      721 tccttacaac tgattttatg gctatttccg catactggat tttagtagaa aatttattca
      781 ggagtatgtt cctatccaaa tctactactg caacgccttc acttatctgc aagctcttta
      841 tcatcttatc attaaagccc aaacctattt cttccgcacc ttcaatcaag tgttgtaatg
      901 ccgctttcgc aagctccgaa gctttaactg aagatgccga tgagccatct attttgtcag
      961 acttgattgt tatcggaact aagtattttg ctgtcttgtc cgggtaatat atcgtgatgt
     1021 acttgtctaa ttcatcagga tttgcctgat cctgctggta ggtatcagga tctatcaagt
     1081 ttatatggct gggccttttt aaggaatccc cgtagggctg tccgttaata gaaatattta
     1141 cgttaatgat attaggacaa tcggtaaggg ttaaaacgac ctgttttctg gcaagttctg
     1201 ccctttcccc ctggaaggga ttgcctgaaa tatccagaga aaccgtatcg ccacttattt
     1261 tcatggtatt taagccggtg ctcagcaagc agctatcaaa accatcaggg aggatcccgg
     1321 aaaataataa gttaacagca gcatttatct caagttctga gtcgtcgata ctataagtca
     1381 ccggcgtcag gaatccatct tttatgtaat agaccattac ttttttcccg gattcaataa
     1441 tatcttcata tgctgaatcc tcattatcat tggcggactg tgcactgcaa cctataagaa
     1501 taaagacaat aaaaagagat aatacggcgt gcagtagtct tctcacaaaa agccctccaa
     1561 aatttatatc gttttctcta ttttaccatg aacgacattt tttttctatg gggaaactta
     1621 gtattttttc cctcctgttt taaaagtctt tcccgaagcc agctttcatt tatgcgataa
     1681 ctcaaagtga gaagactatc aatgagatgc acgttttcca ctgttatatc cttattactg
     1741 acattgattt ctgttggagt aaaattccaa attccacgga tgtccgtatt aaccaacacc
     1801 tcaacaattt tttcggtcac atctcccggc accgcaagca ccgctatatc aacatcattg
     1861 ctgtttataa aatactccat gtcatccaca tggtatatgg gtatatctct aatcttgcga
     1921 cccacgatgc tcggattggt atcaaaaatt gcgataatat taaagccatg tgttgcaaaa
     1981 ccgctataat ttgccagtgc ctgacccagg tttcccgcgc caattagtac cattttatgg
     2041 gaagttgtca gccccaatat tttggcaacc tcattataaa gctgggaaac attgtagccg
     2101 tatccttgtt ggccaaattc accaaagtta tttaaatcct gcctaatctg agatgcagta
     2161 aaacctagta gtttgctgag ttcctgcgac gacacccgct ctacattttt ccggagaagc
     2221 tctccgagac atttatagta ttttggcaag cgcttaatca ctgccataga cacctttttt
     2281 atttcaccgg gctgctccac aatatcaccc cgcttgttat taatttaaca taaatatacc
     2341 ataatatttc actgtagtca aatatttcga atattagtac tgtattcagt catcataaaa
     2401 gcaggcggct gccgaatatt atatttatgc atattcgaga aaggatgttt tggcgatggg
     2461 ccactggctt gtaaacacgg tccttttaac cgcaacaaat accataaccg gcacgattgg
     2521 ctttttttac aggcttgttt tgtcaaaata tttgggttct gaaggcatgg gcgtttatca
     2581 gcaaaccctg gcctttttcg gtacagcaat aactcttgtg acagcaggta ttccattgtc
     2641 ggtatccaaa ctcatagcgg aaatgcagac tgaagctaat aaaaggaacc acatcatctt
     2701 gtctgctttc accctgacaa ccgccttttc tctgctggga gcttcttttc ttatttccct
     2761 atctttcctt ctcaaactga aactgctgct tatagtgttg cctgcagcaa tatttgtagg
     2821 ctattcctcc gtcatgagag gatacttctt tggcatgcag gatactaggc cggtgcagtg
     2881 gtccaacatt actgaatgca tatttcggac tttgttcggc gtgctttttg taagtagcaa
     2941 tttgcttatt ggatttgaaa ctaagactag gggtgcagtt actgccctga cccttggaga
     3001 attcatatcc atgtgcatac tatttatttt ttttgcaaag agcataggca attaccgtaa
     3061 ttccagaaaa aattctgtaa ttgcaacaat caaagatatc ctgaaaatag ccgtcccggt
     3121 ttcatttagc caaatcattc attccatttc acgttctatt gaagcactat tagttccgaa
     3181 aggactagtc ctgtcgggac tttcatctta tgagggctta gccctttacg ggaaaacatc
     3241 gggcatggta atgcccctga tcttcttccc tgccctattt gtaagggcct tgtcatcaaa
     3301 tatcatccct caaattgcaa aggctttggc tctaaagaac aaagagtatg cttttaagct
     3361 atctcagcag gccttgtttc tcacatcctt cttcgccttc gcagtgacgg cattttttat
     3421 tgccctatca gaacctgtgg cagaattcct atttcccaag tttgaactgg gaaatcttat
     3481 tatagctttt tccgccggca tcccctttta ttaccttgaa agtatcataa tgtcaatact
     3541 tagggctatc ggcaacaaca gcacccccat tacaacatcc ctcactactt tgtttataac
     3601 aaatcaaatc ctgtttttgt taacggctaa aacacccatg ggaatttacg gttattccgt
     3661 tgccttaatt accgcttctg cagtggcaat atttataagt attcggcata tcgagcaaaa
     3721 ctttcaaaga caatttaacc tgctagagat tatattaaaa cccatgattt gctgcgtttt
     3781 tatggcctac ataatgagca aagctttttt gcccctggta aatttagact tcccaaaaat
     3841 aatatgcata ggggcagacc taattctagg aatcagtggc tacttaatac ttgccctggc
     3901 tttgggaata gacctaagag gcataagaca aagatactga ctttacttga gctagaacat
     3961 ccggcaccca ttatataaaa acgcaataca atgtattaga agctggtgtg gaggggatgc
     4021 aatgaaaaca attagactat ttcaccgatt atcaagcggc cagtacctgg caatgtatgt
     4081 aacacggcac cgacagcggt caaagagcgt cgcctatatc gtggcagtgt gtattttccc
     4141 cacaaaaagg gagtgcaatt attggttcag gcatcaagaa gagctaattt cgaaaagctt
     4201 caatacttgg ggcttggagg gcatgttgaa ggctttaaaa tggctaaagc agctgaaaca
     4261 tgccattggc cccggcgaat ccattgtaat ttactgggtt gacgagcgca gacgtcgggc
     4321 tttcaagttc cttgaaagat acggatttaa acagggtgaa tatctaggtc gtccatgtta
     4381 tatattcaaa aacagcggca gctctttagg ttaaaacaag gagcaaagta gctactcctt
     4441 gttttcaatc ctcgttatgc ttttaaacat taaatctgaa acttataata tctccgtcct
     4501 ggacaacata gtcctttccc tctaagcgca gcttgcccaa atccctggcc ttggccatag
     4561 aaccgcattc cttgaattcg tcaaagctta ttacctctgc ccttataaat ccccgttcga
     4621 tgtcagagtg aattttacct gcagccttct ttgccactgt gcccttcttt attgtccacg
     4681 ccctgacctc gtcttctccg acagttaaaa atgatattag gctgagccta tcgtaaatgg
     4741 cccttgcaag gaggtctatg ccggactctt taatatttaa ctccctcata aatgcaattc
     4801 tatcatcgtc atccaactcg tttatttcca cttccgcttt tgcactgacc tcaaataccg
     4861 ggagattctt ctcatgacag tatcttaaaa cctcgtcttt ttgctcgtaa ccttcaatta
     4921 gctgctcatc gtccacattt accactatga ttaccggttt ggccgttaaa aaatccaagt
     4981 gttccaaaag cttttcttca tcttcggaaa gattgaaatc ggcaagactc atttcatttt
     5041 ccagggactc acgaatcttc tcaagcactt ctctttcagc tacctgctct ttggtgactt
     5101 ttcttccatt atttattcta tcgattctag tttccacaag ctgtaggtcc gccaggagca
     5161 gctccgttcc gatattctca atgtccctta ttatgtctat cgaaccttcg gaatgaatta
     5221 cattttcatc cctaaaagcc ctcactatgt ggaccaggca atccacctgc cgtatgttgc
     5281 tgaggaattg atttccggaa cccttacctt caccggcccc ccttgtcaaa cccggaatat
     5341 ccaccacttc aatttgagca taggtgacct ttttgggttt gtaaacctgg gctagaaaat
     5401 caattctccc atcaggcacc ctggcaaggc ctatatttgc attttgcttg ccgctttgaa
     5461 aggcattcgt ctctgccttt gcatgagtca ataaattaaa aaaagtggtt ttacccacag
     5521 taggcaatcc aactagtcca accttcacag caacatgtca ctccttctaa gccattacaa
     5581 ttaatggaaa ttacaaaatg cgaattctgg gaaatattat catcacaaac tttattttag
     5641 acaccttttt tattttagct ttataaacta ttaaaatcaa caataatgta ttataaaaac
     5701 aaaaaacgta gcaacggttt tccgttctac gtcctgattt catttggtgg agaatagggg
     5761 agtcgaaccc ctgacctcct gaatgccatt caggcgctct cccagctgag ctaattcccc
     5821 atctaataag tttaatcaca agcatttact agtataatag ttttatttga tattgtcaag
     5881 gctagtttat acacattttt atcatggtgg gcgcggcagg tttcgaacct gcgacctctt
     5941 gaatgtgaat caagcgctct cccactgagc tacgcgcccc ttttggtgga gataaggggg
     6001 atcgaacccc tgaccttctg attgcgaacc agacgctctc ccagctgagc tatatcccca
     6061 ctaatagcca attgccgtga aaatattata atacaaagct aatgaaaatt caagattgta
     6121 attttattta agggcaccaa ttcgatttat tggccatatt cggtacgttg ctttgcctac
     6181 gatttgctct cttgaaacat atttgttgtc ccagaacctg ctgtcctttg aattgtttcg
     6241 gttatccccc aacataaagt aatgcccttc aggaacttta tagggcccga aatcattgta
     6301 catggcttcg tttaactggg gctcctgtat gggttcatca tttctataaa ggacactatc
     6361 cctaatctct attacatccc cgcccttacc tataaggcgc ttgacaaata tctgcttatg
     6421 gtcatcggga tatctaaaga ccactatgtc attatattca ggagactgaa atctatatat
     6481 atacttattt accaaaatcc tgtcgttaat ttggatagtt ggaatcatgg aaccgctagg
     6541 tactatcatg ggttcaaata taaaacctct tatgataaat gcaagaataa gtgccaggac
     6601 aatagccttc aaccattcaa atatctctct cttaatgtca ttgctcatgt cttcacctgt
     6661 aaccttaaaa tttctgttgc gataattcac ttattaatta taacacaaaa tattttttta
     6721 caaagccttt tgtgaaaaat attctttaat cgcaagcgcc attgcttttg ctatttgctt
     6781 ttgaaaagct tctgttgtta gaagctgcct gtccccggaa ttagttatga aacccagttc
     6841 cacaatgaca ccggctggct ttgtgttttt caatataaaa agagatctgt tgccgttagc
     6901 tttccgtgcc ggtaccctgt ggccagcttt aatgtaagct tcttctaagt ttttttgaat
     6961 ctgcagagcc aattgcctgc tttttacact gtcataataa taaaagacca tgggcccccg
     7021 agttttagct gagctttttt cagcattcac gtgaaggctc aggaatatat cgggtctgga
     7081 attatttata atgtcgactc tcgcaatcag gtccctcttg tgcctatact cactcttatt
     7141 gttcaaatgg tccaaggcaa tatcttttgt cctggtcatg accacattgg caccttcttt
     7201 ttctaagatg ctctttaatt tcaaggaaac ttcaagattg atatgtttct ctaaaacgct
     7261 atcaaagctt gctcctccat cgataccgcc gtgaccgggg tcaatcacta tcaccttccc
     7321 ggacaagggc agccaaccgc ttaaaacact taccgcaaga tttttgccgt ataaatattg
     7381 gattatcagt gccaatataa gtaaagtcgc taatacaaag gtacttttgc cgggaacctt
     7441 taattttaca cgttccacac taaagccccc ttttgccagc gttgacaaat actgttctta
     7501 ttaaacatat ccttacatgt atggaaatat gaccgttgct ggatatttaa gctaacggag
     7561 cttgtgaaag agaacatttt ctttattgac ggaatattct tgatttttta aacgctttat
     7621 gctattatat aacaaaaaaa cagaaggaga tgatttataa tggcaatccc gagaaagatt
     7681 aattttactg aaattgcaac ttgtaaacat ttaaaatatg aggaaacatg ctacgggcct
     7741 attcaatatt gtagcttaca tgcatggggg aaccacgtgc cctgcagcga agacataaaa
     7801 gaaatctgcg aaaaggacca ttatatcatt atgtaggttt cttgccgaac ctgaccacat
     7861 atctcatctt tccatctctt acgaacttat ctacccaggt atcaatttct tcaacatcgg
     7921 taagataaac cttgttcttt ttcaaatcct tttccagagt gaacttgacc atgagccctt
     7981 cctcggtata aggaaatctc tgtgccgata tgaaattgcc catcgaaaac gcaatgtatt
     8041 tcttaattat ttctccatca gcttcctgga cctccatcca ttctccaggc tgaataacgt
     8101 gggggtgact tgctacaata aagcttgctc cattttttag gagaaggtga gcaagctttc
     8161 tctgatattt tgaaggagtc ctctggtatt catcaccata atgcaggtac accacaacca
     8221 catcagcctg gcttcgcgct ttttctatat ccgaaagtat caactcttca tcaatcagat
     8281 taacaaggta cggcatgtcc ttcggaatgg gaatgccgtt tgttccgtac gtgtacgaca
     8341 aaaaggcaaa acttatgccg tttagttctt ttatcaggat ttgctcacgt tcctcttcag
     8401 attcataggt gcctataggg gccaatccgc gttctttaag gacctgtatt gtcctgatgg
     8461 ctccttttgc ccctctgtcc aaactgtgat tattggaagt tactataata tcaaagccgg
     8521 catctttcag tgcatcagct atttcatcgg gactgttgaa catgggatag cccgtatatt
     8581 tttgttcctt gcctgccaaa gttgtctcta gattggccat ggcaatatcc gctgcggaaa
     8641 tctcatcttt taccacatgg aaaaactccg tgtaatcaaa gctatccgca gcatcactgt
     8701 atcccgacca aatctggcca tcatgcatca tcacatctcc cactgccaga aaagttgctg
     8761 tgggatttgg tagatctact tcttctggtt cctcttgact taattcttct tcggtgttca
     8821 catcatcgct atccgcattt ttgcttgtag ttacgggttg ccaggttgca agtacactta
     8881 catagtcatt aaaagcatct ctatagacca acatgacacc cgcaaaaatt attaatgcca
     8941 aggcaactaa atagggccaa agctttcctt tcttattgtt cattacagca gttcctctct
     9001 tcccacttta ttcaagtatt ccactacctt ttttacatcc tgagcttttt cttttgaaca
     9061 taccagtaag gcatcgctgg tttccaccac aatcatgttt tccaaaccta ccgctgcgac
     9121 aaatcggctg ttagaataaa tgatggaatt gcgggtttca atatcaacaa aatctccttt
     9181 tgtaatgttg ccgttttcat caggagggaa cacgcttcct agagaatccc agcatccgac
     9241 atcattccaa ccaaaatccc ccggaataac aacaacttcg tcagaccttt ccatgacacc
     9301 gtagtcaatg gaaatgctgg gcaatatagg atatatgtct tcaactgcct ttccttcctc
     9361 gggagtacct agaaaaggct ctattttctc tatatttcta tataggcggg gcaaaaacct
     9421 tctgaaatta tttaaaatga ctgaactttt ccagacaaac atgccgctgt tccaaaggta
     9481 gttaccgctt tccaggtatt ttttggcttt aggcacgctg ggtttttcta caaattctac
     9541 tgcttcatat gccgtcttat ttcccgcagg taaagaccta tccttgtcat attttatata
     9601 gccgtacccc gttgagggat aggtgggctc tatccccata gtgacgagtt tttccgtgct
     9661 ctctgcaatg ctacaagcag accttaagac cctttggaac tcatccacat tttttatgta
     9721 gtggtccgag ggaaagacgg ccattaccga atccccgtat ctcttcttta tgacaattgc
     9781 agcatatgcg atacatgctg ccgtatttct gccaacaggc tctaccaaaa cattttcctt
     9841 gggtatatct tcctggatca cttcattgag tattttttcc tgggctttgt tggtgacgat
     9901 taatatattc tccggcggaa taacatcttt tatcctcttt atggtatcat ttatcatgct
     9961 gtcttcgccg gtgatgttta aaaattgttt cggagcggtg gcacgactca aaggccaaaa
    10021 ccgggtgcct cccccgccag ccattattac tccatatatg ttcattgata tcctccagtg
    10081 cttttattat atttatgttt attttgccct gcttttccaa atatctttca aaatcagagc
    10141 taataatatg gcaaaacacc gaaagatatc aaaaaaaaga aacttcaaca gcctttaaac
    10201 tgaagaagtt tcttttgcag tcaatattaa ttcatgtagt acttgaacag tgcttgcgta
    10261 ccgctatttt cctcgccccg ctccgccaat tcttcgtata tggatagggc aagctccagg
    10321 ccgggagttt taagtccgag ttcctttgcc gattccaggg caattttcat atcttttata
    10381 aagtgtttta tgtaaaatcc cggctcaaaa tctccagaga gcattctggg tgtataattg
    10441 ctaagagacc agctgcctgc agccccagac tctatgctct ttaagaccac cttcggatca
    10501 aggcctgcct ttatggcata ggccattgct tcacaaacgc ccagcatatt agaggcaatg
    10561 gcaatttgat tgctcatctt tgtgtactgc cctgctccgg cgccgccctg caggacgatg
    10621 ttcttgccca tcacttcaaa aattggcttt acttcctcaa aggcttcggc atcaccgcct
    10681 accatgattg atagcctcgc ttccttagct ccaacatcgc cgcccgaaac cggtgcatcc
    10741 agcgcatgta agttcctggc ctttgctgct tcgtaaatct ccttggccag tttcggactg
    10801 gatgtggtca tatcgatcag gtaagagcct gcttttgcat tttctatgat tccgttttcg
    10861 ccaagataga cttcccttac atccttcgga tatcccacaa ttgttatgat gacattagct
    10921 tcacgggcaa cttcggccac cgtatccttc caggtcgcac ctttttcaat cagttcctca
    10981 gcttttgcct tggtgcggtt gtagacaagt accttatagc ctgctttcat caaattcatc
    11041 gccatacttt ttcccattac ccctgtgcca ataaagccta taagggtatc ttttttacat
    11101 agcgtcatac ataaggcccc cttgttatta tgtttttgct gcttctaata ctcctttcgc
    11161 agctttgata acgcttccga ataagccttc atagttttct catcaaaaca aaccattaga
    11221 actttttcaa tgctgttgtt ttcctctaaa aaccttgcta tctctctaat tgcaatgtct
    11281 gcagcccgct ttaccggaaa accgtatgcg cccgtgctaa ttgacggaaa tgcaattgtc
    11341 ttcacattgt tttctacggc cagcattagt gaactgcgat aacatgatgc cagcagctca
    11401 tcctcgttgg catcccctcc ttgccagatg ggccccaccg tgtgaatcac gtgctttgcg
    11461 ggtagcctgt atcccttcgt aattttggcc tgtccagttt cacagccctt taattgccgg
    11521 cattcttcga gaagttcggg cccggcagcc cggtgaattg ccccgtctac accgccaccc
    11581 cccagcagcg ttttatttgc tgcatttacg atggcatcaa cttccacctt ggtaatatca
    11641 cctaaaatca catcaatccg ttgcaaaaat cacccctccc atcggtgcct ggcacctaaa
    11701 gtttttaagt tattgaatat ttagggacag tccccaatta ttcatttggt tttacccaat
    11761 aagaatggcc gcggtcaggg ttaagcccgt catttgatat acctgtattg ttttggcatt
    11821 tgcctcaacg agtctggcta tgtcatcgta gtattttttc gccacatcga cagagcgttt
    11881 ggctatcgga gcgctcagca gggctatgag caataaagga tttcctgtaa agaaaactgc
    11941 gataagcaga cttaaatagg ctaagacgta taataaaata taaactacaa tccctttttc
    12001 ctttccgagt ctcacgaccc aatttcgctt attgcccctt gcgtcggcct cataatccgg
    12061 gtactgatta atccacacta cattggctat taaaaaacct atcatcaatg aaagcagcag
    12121 tatctccaag ttgacggtgt gtgtctgcac aagatatgtg ccggatacaa caagtggacc
    12181 ataagttaag cctattgcaa attccccaag acccctgtag caaaacttaa atgggggaag
    12241 gctgtagaat attgatatca aaaagcccgc aatgccgatc cacaagacca tcggctctcg
    12301 tgcaatggca atgtaaagac caatggccgc tgcggcaagc atggtaacaa gggctattat
    12361 cttagtttcc tcaacagtca aaaggccgtc tacaattgtc ttcttcccgc cgctaaaagg
    12421 tgtcctctta tcgggagtta caaaacggtc cactccggat ttataatcca caacttcatt
    12481 tatggcattc ttacccgtct caattaagta aacgccaaaa aagctcaaaa acaaccaata
    12541 aatgtcaatg taccctaagg ccttgtaagc cattgctgta cttaccacca tgggaaccgt
    12601 cgatgcaatg cagagtttag gatctgctag ccgccaaaca ccctggatta gcctggaaaa
    12661 gctgcttgcc atttaaatct tacacctctc gcccttaatt tcctattttt tatatgtata
    12721 ttataccaat ttaagtaaaa aaatttaaag atttttggga tcttcaaaag cgtcaacttc
    12781 atttagtaga ttttcacgtc attaatgaag tttacctgcc gctgcaaccg agctttgatt
    12841 ggcaagttga aagcagcttg ctccgtcacg atgtgaaatg agtttttcta aaaatttcgc
    12901 aaggccttca tcgtcattgc tatccgtcac ataatctgcc ttgcttttaa ccagttcggg
    12961 agcattgccc atggccactc ctaaacctgc gtacttgagc atttctatat cattataatt
    13021 atcgcccacg gttattattt cgtcatgact tatgcctagt gtttcggcaa gttcaattag
    13081 acctttggcc ttggacacgc caccctttaa aacatcgatg caatttggta tggaagaagt
    13141 tattgagatc tcttctccgt atttagccgt gattttattt ataaaatctt ttaaatcacc
    13201 aggcctaccg ttgctgtaaa tgaatacctt tgcaaccggc tcttcgagcg cagaaatcac
    13261 tgtctgggtg cttcccatgt ttttaatcgg cataaaagcg aaatctctca gcaggtttaa
    13321 ataaccttta aaagtgtact ttagtggcac gcggaaaaat cgtttgaaat attggccttg
    13381 atatcttctg ccgccgtata tctttccgct tttaacaaaa agcagtactt taaaaccttc
    13441 gtagtccagc gtcattctca taacatctac cgctatctca tgaggcagca agtgataagt
    13501 cacacaactt gaatctttat ccgtaattat tgcaccgtcg cagcacacca tgggagcatt
    13561 gactccgatt atccgtgcca acctacgaac actgtgataa tgccttcccg tagctatggt
    13621 aacccttatc cccaaatcct taaccttttt aatcgctttc agggtgcggg gggtaaccaa
    13681 gtggccgcta gttattaatg tgccgtcaat atccagggct agtaatttgt agtttttgtt
    13741 aagcaatgta tttaattcct tttgtgtctc cattgacttc atcctttgtc ttttaaagca
    13801 ttaatcattt gttattatat cttattagca actattttca aataacaaga cagtaatgat
    13861 ccaaaatgat tcttatttca tcgtttttat gcctttcaag gtcggagcgt tgttatcgct
    13921 tttgcccaat ttgcgtcgat tcagcttttc atcatttaag agttttgctc gaaatataac
    13981 tccatcgcca aatctatcct ttatttcgtc catcaccttg tttaatttct tctttttttc
    14041 atccgtgtca aagagggaca cctgctcaaa ttcctttacc aactgggaaa gactgactcc
    14101 caaaagtcta aggggtgttt ttccatccca gttggaatac aaaagctttt tcgccaaatt
    14161 gtagatatct tcagtgctat tggtatagga aatggtggcc gaacgggtaa tggtgcaaaa
    14221 gctattgtcc ctcaaagtaa ttgtcaccgt gcggcccatg taattttccc gtctgaccct
    14281 tctacccacc tgttctgaaa gagataataa caccgtctcc gcttcatcat aggatgttat
    14341 gtccttggga agggttaccg aatggcccat ggatttggcc tcatccatag cgtgggggtc
    14401 cacaggactt gtgtcgatgc cgtttgccca aaggtgcagg tatctgccat ttaagccgaa
    14461 agctttgccc aggagtcctt gagggacccg ggccagatcc ccaatggtct ttatattcat
    14521 gtggctgaga tgctccgcca tgcgccggcc tacaccaaat aactcgtcaa cgggcagagg
    14581 ccatataagc ttgggcacat cttcaggagg cagcaccgta acggcatcgg gctttttcaa
    14641 gtccgatgcc attttggcca aaagcttgtt gctcgatacg ccaatggaac atagcaaatc
    14701 cagttccgac ctaattgcag cctgtatctt atgggcgatg gtcacggaat cgccaaaaag
    14761 cctttcgcat cctgtcacat cgagccaggc ctcatcaatg ctgaaaacct caaccaaagg
    14821 tgtaaatctc cccagaattt ccatgacctt ctggctagtt cttacatata attcgtagtc
    14881 cggctccaca aaaatagctt gcggacatag ctttcttgcc tgccagttgg gcattgccgt
    14941 cttaactccg tatttgcgcg cttcataaga tgccgtcaat attataccgg tgcgcttttt
    15001 agggtcgccc gccaccagga taggtttgcc cttaaggctg ggatcctttg cctggtgaca
    15061 ggaagcataa aaagcattca tatccacatg caatatggga agcatagcac catctccgaa
    15121 catgtgtttt gtcctttatt ataccaccat tctatagtaa aatcaacatg acagcgagcc
    15181 cacacctgac aaggacacgg gtaggcttca ttcgccatgt cggcaatttc agcttcgtgc
    15241 ctggtgccaa acctcatcct agaccatagt atgcctggca attagattat gataacggca
    15301 tgacaaagtg attttgcttg tcatgtgata tccggtgaag taacggtagt ctcccaaaat
    15361 caagacctgt tgttctaaag cttggccctt cactccgcat gggttcacgg gcaatttggc
    15421 atcctgccaa gtgcccaaag c
//
LOCUS       DAONUO010000006        14825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_3038_length_14825_cov_3.977183, whole genome shotgun sequence.
ACCESSION   DAONUO010000006 DAONUO010000000
VERSION     DAONUO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 14825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14825
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_14825_cov_3.977183"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK768_00505"
     CDS             complement(<1..448)
                     /locus_tag="PK768_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HQA59484.1"
                     /translation="MNLKQEIIDFAKRIGFDKVGFTTAEPFLEEKELLIEKKAKNLLS
                     PFEEHDVQLRCDPNKILPAAKTIICFAMGYLMEPYEPAETAGDQESGQLIAKISRYAR
                     VRDYHYVLTEKLEKVVEFISSKRAGQFKIMVDTGSLLEKAAARRAGL"
     gene            540..818
                     /locus_tag="PK768_00510"
     CDS             540..818
                     /locus_tag="PK768_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HQA59485.1"
                     /translation="MISNEQIARINELYRKQKSQGLSEEEKKEQVYLRRLYIDAVKAN
                     LKAQLDSIKKAKKSNCCGHHNHNHNHSHGHNNCGHPHHHGHGCDCDNH"
     gene            complement(815..1330)
                     /locus_tag="PK768_00515"
     CDS             complement(815..1330)
                     /locus_tag="PK768_00515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HQA59486.1"
                     /translation="MKALLVIDMLVDFIDEKGALSVGPAGQKIVPFIKERIEEFRKNG
                     HPVIFICDNHEKNDKEFDMFPAHCIAETAGARIIDDLDVNDEDKIIRKRRYSAFFGTD
                     LDLYLREKGVDELYLVGVCTNICVLYTAADARNLAYNVNIYKDGVASFDEKANDFALK
                     EAENTLGCRII"
     gene            1518..2201
                     /locus_tag="PK768_00520"
     CDS             1518..2201
                     /locus_tag="PK768_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HQA59487.1"
                     /translation="MQYRKDELVFGIKRALPIVMGYIPVGFALGVMAADAGLSPLQMG
                     LMSLFVYAGSAQFIAIDMLSSGVTAVPIIITTFLVNLRHFLFSASLAPYFRKIKQGFI
                     PLISFFITDESFAVSITDAEQRPLSHFYYFGLYITAYSSWVVSTVIGATLGNLIPDTK
                     ALGFDFALPGMFLALLCMQMKNARFVLIALISGFLSIFFIYTIPGNWNVILATIIAAM
                     IGVFLGDEN"
     gene            2191..2517
                     /locus_tag="PK768_00525"
     CDS             2191..2517
                     /locus_tag="PK768_00525"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HQA59488.1"
                     /translation="MKIDVNYIYLILAMGFVTYLPRMLPATLLSKRKIPDFFVKFLSF
                     VPTAVLSALLFPGVLIVENKLNIGLSNPLFIATILTFPLAYKTKYMFATVLVGMGVVV
                     LHNYFV"
     gene            2544..3674
                     /locus_tag="PK768_00530"
     CDS             2544..3674
                     /locus_tag="PK768_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQA59489.1"
                     /translation="MEIKTFAVERWMNTYENDAVYNLAETDAKPFTLDELLSLGNKDE
                     LIEELLKLKLGYNPTTGRQELREVIAGFYRDTQPENILITTGAIEADFLVTNVLVKPG
                     DTVIVQFPAYQALYSTAEARGARVKYWKMDIDKGYEPDIDELKSLIDEKTRFIVLNIP
                     HNPTGAVISESQLKTILSWAEEGDFWVLCDEVYHDFALYPNIIPAHGRSLSKRAISVG
                     SMSKAYGLSGLRLGWIAGPKELVNACWGWKDYTSISNSPINDFLATFALKNVDKVMER
                     NLSLARANLDIFLKWLEEHKDFFEYVEPKAGVLLFPRLKNIDITTEELCKRLYEKHKL
                     LMLPGECFEMPGFLRIGFGNDSDMFKTGLSIFSAFLKSQSGI"
     gene            complement(3986..4070)
                     /locus_tag="PK768_00535"
     tRNA            complement(3986..4070)
                     /locus_tag="PK768_00535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4034..4036),aa:Leu,seq:gag)
     gene            complement(4121..5734)
                     /locus_tag="PK768_00540"
     CDS             complement(4121..5734)
                     /locus_tag="PK768_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA59490.1"
                     /translation="MKAKKVIILFLVLTFFITACSETSLNPVEQNEAVEQENPGPPQS
                     GGTVNLGITSALNLNPLLANDDETMAIQSLIFEGLVKIDAQGMVQPALAESWQISDDG
                     KTYTIKIRSDVTWHNGQTFSSADVKAVFDKIMELKRQKGNKDLPAYTEFDNVESYEAQ
                     DKNTLVITLSKPDADFLYGLNRGIPWAGFLEADARENEMEELVGTGPFKVVDRTSNLV
                     TLNRNDDYFGNVPYIDEINIKVYPDSTSLKEAFAIGDVDLLAIEPIDWNVFEDMENVY
                     LLQYPSRYFEFIAINLTNPILNDVNVRQALLMGIDRNRILQDTTLGRGIVIDGPILPF
                     SWAFNSQIRHVTYSKKMAAQQLEEAGWIDEDDDGILEKTIGNKKYKFEFELLVNTDNP
                     ARYQTASHIEKNLKDLGISVKLVNVTWDELVQRVMGKKYDAAIMGWMLASNPDLRFMF
                     ASSEIKSGYNFVSYSNPQLDDVLTRAATDHEGRRELLYKAQEIINEDLPYLFLYSPNK
                     VLAINKKIQGVKPNPVNIYYDISDWWVDR"
     gene            5945..6352
                     /gene="ndk"
                     /locus_tag="PK768_00545"
     CDS             5945..6352
                     /gene="ndk"
                     /locus_tag="PK768_00545"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQA59491.1"
                     /translation="MEKTFVMIKPDGIKRGLVGRIIERYEQKGLKISAAKLVNATIDL
                     AEKHYAEHASKPFFSELISYITSGPVMAMVLEGPGAIKLARIINGATKVEDALPGTIR
                     GDFATSTTENLVHASDSLESAQREIALWFPEIE"
     gene            complement(6358..7392)
                     /locus_tag="PK768_00550"
     CDS             complement(6358..7392)
                     /locus_tag="PK768_00550"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HQA59492.1"
                     /translation="MEEIKTLRDVKNIKVDSKRRLHSADHDEILAGMTTDIYFIKTME
                     ILRHMGLSQKEVVAEVFPRRPGVLCGTQEVLNLLHDKNLEIYALPEGETFEAKETVMR
                     IKGSYEEFGIYETVLLGMMAHPSAWATAARECREAAGQRPFICFGARHVHPAVAPVME
                     RAAVIGGADNASCILAAKLLGREPSGTIPHAAMIVAGDTVSVAKTYNEIMPDDSPRII
                     LVDTFKDEAEEALNVAEALGKDLEGIRLDTPSERGGVTPGLVHEIRNRLNQKGYDYVK
                     IFVSGGLTPERIRTLIEAGADAFGVGSYISGATPIDMTMDLKMVDGTPVAKRGRIPGL
                     QDNPKLVRIK"
     gene            complement(7493..8035)
                     /locus_tag="PK768_00555"
     CDS             complement(7493..8035)
                     /locus_tag="PK768_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQA59493.1"
                     /translation="MSKKLVAVLVIILGFAALYNNIGWFMRYIYPLKYEQSIVSHAKE
                     YDVDPYLVASVIKVESNFSPKVVSKQGAIGLMQIMPKTAIWAAQQMGIEDFQIEKLQN
                     PDLNIKIGTWYLSSLLREFDNNTILALAAYNGGRGNVAKWLAQGRMQQNEDDIPFPET
                     RNFVMRVKRSYKWYRRLYNL"
     gene            complement(8017..8631)
                     /gene="coaE"
                     /locus_tag="PK768_00560"
     CDS             complement(8017..8631)
                     /gene="coaE"
                     /locus_tag="PK768_00560"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276412.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQA59494.1"
                     /translation="MFVVGLTGGIASGKSTVSLLLKDKGAVIIDADEIARQIMQPDKP
                     AWFKVINHFGHEILNEEEYIDRKKLADIVFSDKRQLEFLNSFTHPEIINEIKSQLEYY
                     KSLQKEVVIIDAALLLEVGLDAIVDEVWVVTVDEKVQLERLMNRDKNLNKEQAMSRIR
                     SQMPQEEKIKYADRVIDNSGSIEDTKVQVDSIWREISRQCQKNL"
     gene            complement(8644..11229)
                     /gene="polA"
                     /locus_tag="PK768_00565"
     CDS             complement(8644..11229)
                     /gene="polA"
                     /locus_tag="PK768_00565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQA59495.1"
                     /translation="MRKMLLIDGNSLLHRAFHALPPLRTSKGVHTNAVYGFLNMLFRI
                     LQEEKPDYISVAFDKKGPTFRHDEYVAYKATRPKTPEELAGQFDILKDILKALNIHYF
                     EVDGFEADDILGTMSKKGEEAGVFSVIVTGDKDALQLVSSNTSVMLTKKGISELEVYD
                     PAKVKERYGIPPERIVDMKGLVGDKSDNIPGVPGIGEKTAAKLLRDFDTIDNILANTE
                     KLKGKLKENLEVYHEQAKISKYLATIVTDVSVDFSFEDLASREPDYDRLITLFRELEF
                     YSLIDRIKPTDNNDKQESFKTIKINNADDFQNLVSEISLEKLIGVLVDAKAKDLKTFT
                     INGIYISLKDKSYYIDVSLLDDKNLIRQLKSILEDDDVYKITHDGKFLRKVLKQLDID
                     FASQFDVMLAAYLLEPSKPNYDIATLALEYLGEKLDEAADLGEKAAYLKSLKKVLDDK
                     IRDFEMQRLFYEVEMPLSVVLADMEQIGIKVNPEKLKELGARFGEKLDILTEDIYSKA
                     GMEFNINSPKQLGEVLFEKLSLPVIKKTKTGFSTDAEVLEKLRGSHPIVESILEYRFL
                     MKMKSTYADGLLALVDKVTERIYTNFNQTVTSTGRISSTEPNLQNIPVKTEEGRHIRG
                     VFTADGPGHVLLSGDYSQIELRVLAHISEDRTLMEAFIKGEDIHTRTASEVFGVSPED
                     VTPSLRDKAKAVNFGIVYGISDYGLAQGLGISNQEAKEYIDAYFERYPGVRDYVRDTI
                     RLAKLSGYVTTLLNRRRYIPDINSRNYHLRSFAERTAMNTPIQGSAADIIKVAMVKVY
                     RHLREKNLKAKIILQVHDELILDVPEDELSIVKEILKTDMENAIPLRVPLVVDFTQGY
                     TWEEL"
     gene            complement(11363..12637)
                     /locus_tag="PK768_00570"
     CDS             complement(11363..12637)
                     /locus_tag="PK768_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HQA59496.1"
                     /translation="MGCSKHYEDLIDKYIEGLVTLEEKELLESHMEVCPDCRQEAKEL
                     QKIIKASASLGQVELPVDLKANLMGKINTLSYQQRSHEKSLPLLKLIRNIPELLYAYI
                     YHNKRIFVGALSVFLIGIFVVTAYNQGVFDMNYATNSVKEEAVSESAPADMSSMMTMD
                     GAGTSGWMQSEGESIAQVPSTNMADTRLKTFSAEEKSQKIIKNADLSIYVEKFDEKVD
                     YIIKTVEELGGYIEHSQIQGGKSIDSPRRAYMALRIPQAKLNDALDIFKTLGRVTNNQ
                     ISGENITDAYYDTDARIRNLVQQEQRLLEILNMANTIDEILRIEIELNRVRTDIDMLK
                     GQLMAWDKMVEMSLVNVNLIEQEPSKEKVTTVTPLELMQRARQGFITAINISMNMLAF
                     LAELTGALLPVALILGILYLLIRRVLSKRKKG"
     gene            complement(12668..13237)
                     /locus_tag="PK768_00575"
     CDS             complement(12668..13237)
                     /locus_tag="PK768_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQA59497.1"
                     /translation="MNAEKELISRAKKGDVSAFEELIAGYEKKVYNTVYRFFNNSEDA
                     MDITQEIFIKVYTSLHSFREDSSFSTWIYRIAVNTCIDFLRKKKDETLPIKDEMPLGN
                     DLKHSSYTQLPEDFVEKQELKEVLLKAINTLPKEQRICVILRDIQGFSYTEMSEILMC
                     SLGTIKSRLSRGRRALREKLKSPELFLSN"
     gene            complement(13334..14245)
                     /locus_tag="PK768_00580"
     CDS             complement(13334..14245)
                     /locus_tag="PK768_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQA59498.1"
                     /translation="MAKNMLPGYLSLLGNGQLLEKVRQAKQHLSKCNLCPHECNVNRK
                     ENLGVCRAGEMAIVASYGPHFGEERPLVGRNGSGTIFFGYCNMRCVFCQNYELSFRGY
                     GAAVSDEKLAEIMLLLQNHYKCHNINLVTPTHFVPNILEALYIAAQKGLKLPLIYNCG
                     GYEKIETLKILEGVIDIYMPDFKYAFSERGRKYSGVKDYPQKAKLALKEMDRQVRGLK
                     TDERGIAYKGLLIRHLVMPGGLEDTKDVLKFIKEELSADCLVNLMDDQYYPANKAHQY
                     EEISRRLSNREYMEALYYAKSLGLRLA"
BASE COUNT         3966 a         3341 c         2771 g         4747 t
ORIGIN      
        1 ccaatcccgc tcgcctggca gcagcttttt ccaaaaggct gcccgtatcg accatgattt
       61 tgaattgacc tgctcttttt gacgaaatga attctacgac cttttcaagc ttttctgtta
      121 acacataatg gtaatctctt acccgggcat atctggaaat cttagctatt aactgaccac
      181 tctcttgatc cccagcagtt tctgccggct catagggctc catcaggtac cccatggcaa
      241 agcatataat cgtcttggca gcgggtaaga tcttgtttgg gtcacacctg agctgtacat
      301 catgctcttc aaagggcgaa agtaggtttt ttgccttttt ttctataagg agttcctttt
      361 cttcaagaaa aggctccgcc gtagtaaagc ccactttgtc aaaacctatt cttttagcaa
      421 aatcgatgat ttcctgcttt aaattcatat ttatcaccaa ttgtattttt tttaaaaaac
      481 tttataatgg tagtatactc agttttccca tagagtcaaa actcgaaagg atggttttga
      541 tgatatccaa tgaacaaatc gcccgcataa atgagcttta tagaaaacag aaaagccaag
      601 gcctttccga agaagaaaaa aaagagcagg tttacctgcg cagactttac atcgatgctg
      661 ttaaagcaaa tctaaaagct caactggaca gcataaaaaa agcgaagaaa tctaactgtt
      721 gcggccatca taaccataat cataatcaca gccatggcca caataactgc ggccacccgc
      781 atcaccatgg ccacgggtgt gactgcgaca atcattaaat tattcggcag ccaagggtat
      841 tttccgcttc ttttagggca aaatcatttg ccttttcatc gaaagatgct actccgtctt
      901 tgtagatgtt cacattgtaa gcaagattcc ttgcatcggc ggcagtatag agcacgcaga
      961 tattagtgca cacacccact agatatagct cgtctactcc cttttctctt agatacaggt
     1021 ccaaatctgt cccgaaaaag gcactgtagc gtcttttcct tattatctta tcttcatcat
     1081 tgacatcaag gtcatctatt atccgggctc ctgctgtttc agctatacag tgggccggaa
     1141 acatgtcgaa ttctttgtca tttttctcat ggttgtcaca gatgaaaatt acaggatggc
     1201 catttttcct aaattcttca attctctctt ttataaatgg aactattttt tgacctgcag
     1261 gtcccacgct caatgctccc ttttcatcaa taaaatcaac taacatatcg ataaccaata
     1321 aggctttcat gaaatactcc cccttccagg cgtcagccaa atctttcttg tccctttaac
     1381 ttgaaaaaat tataccataa ttggtcatgt tctagcaatt atgcgatttc cgtgacattt
     1441 accaatactc atattgacac aaatcttatt catctgttat cataggattt aattattatg
     1501 cattatgagg gataattatg cagtatagga aagatgaact ggtttttgga attaaaaggg
     1561 cgctgcctat cgtcatggga tacatacccg ttggctttgc actgggggta atggccgcgg
     1621 atgccggctt aagcccactt caaatgggat tgatgtctct tttcgtatac gccggctctg
     1681 cccaattcat agccattgat atgctgagct ctggagtgac tgccgttccc ataattataa
     1741 ctaccttttt ggtgaacctg agacattttt tgttcagtgc atcactggcc ccatattttc
     1801 gcaaaataaa acaaggcttt attcccctta tatccttctt cattactgac gaaagttttg
     1861 ccgtgagcat taccgatgca gagcaaaggc ccctctccca tttctactat tttggccttt
     1921 atataacagc ttattccagt tgggttgtaa gcaccgtaat aggtgcaact ttaggcaatc
     1981 taattcctga cacaaaagct ttaggctttg actttgccct tccggggatg ttccttgccc
     2041 ttttgtgcat gcagatgaaa aatgcgagat ttgtcttgat tgcattaata tcaggctttc
     2101 tctctatatt tttcatatat acaatacccg gcaactggaa tgttattctg gcaactatta
     2161 ttgcagccat gataggagtg tttcttggcg atgaaaattg atgtaaatta tatttacctt
     2221 atactggcaa tgggctttgt cacctatttg ccgagaatgt taccggcaac tctgttgtcc
     2281 aaaagaaaaa ttccggattt ctttgtgaaa ttcttaagtt ttgtcccaac agctgtgctt
     2341 tcagccttgc ttttccccgg tgttctcatt gtggagaata aattgaatat cggcctttca
     2401 aaccctcttt ttattgccac tattttgact ttccccttgg cttataagac aaaatacatg
     2461 tttgcaacag tccttgtggg aatgggagta gttgttttgc ataattattt cgtataagta
     2521 tatcggagga gttgtattta tttatggaaa ttaagacctt tgccgttgag cgctggatga
     2581 atacatacga gaatgatgcg gtgtacaatc ttgcggaaac cgatgccaag cccttcacct
     2641 tagatgaact actatccctt ggcaacaaag atgagttgat agaagaactt ttgaaattaa
     2701 agctgggata taaccctact accggcagac aggaattgag agaagtaata gcaggctttt
     2761 accgtgatac ccagccggaa aatatattga ttacaaccgg cgccattgag gcggacttct
     2821 tggtgacaaa tgtgctggta aagccgggtg acacggtcat agtccagttt cccgcatacc
     2881 aggccttgta ttctaccgcc gaagcccgtg gggcccgggt taaatactgg aagatggata
     2941 tcgacaaagg ctatgaacct gatattgatg agctgaaaag cctaattgac gaaaagacca
     3001 gatttatagt gcttaatata ccccacaatc ctaccggggc cgtgatttcc gaaagtcagc
     3061 ttaaaacaat tctctcctgg gctgaagaag gggatttctg ggtactctgt gacgaagtat
     3121 atcatgactt tgccttatac ccgaacatca tcccagccca tggcaggagc ctttctaaaa
     3181 gggccataag tgtaggcagc atgtcaaaag cctatggcct ttcaggcctt cgtttgggtt
     3241 ggatcgcagg acctaaagag cttgtaaacg cctgttgggg atggaaggat tacacgtcaa
     3301 ttagcaattc tcccatcaat gattttctgg caacttttgc ccttaaaaac gtagataaag
     3361 tcatggaaag aaacttaagc ttagcaagag caaaccttga tattttctta aagtggttag
     3421 aagaacacaa agatttcttt gagtatgttg agcccaaggc tggcgtactg ctatttcccc
     3481 gcttgaaaaa catagatatt acaaccgagg aactgtgcaa aaggctctat gaaaaacata
     3541 aactgctgat gctgccggga gagtgttttg aaatgcccgg atttttgcgg attggctttg
     3601 gtaatgacag cgacatgttt aagacaggtc tttccatatt ctctgcgttt ttgaagtcac
     3661 agtcaggaat ctaagcggat aacataatac ctccaaagca gatactctaa ttgatggaat
     3721 tagatgggaa cctctcccct ttgagtaccc tgtagttttg ttgttccagt tgtcgtctac
     3781 ttgataggag taattcatca tctgcatgga gatagtctta tattgccact ttactagaca
     3841 agacgattat agaccaaagt tgtagaggac tgtctgcaca aaactaaaag gcagcaagta
     3901 ttcggcttaa atacctgctg ccccaactct aggtaaacta acaaacaaaa ataaaaaagc
     3961 cctgttgggc tttatttctt ttttatggtg cggagagcgg gaattgaacc cgcacgggat
     4021 aacccatacg cccctcaaac gtacgcgtct gccagttccg ccatccccgc acagtgcatt
     4081 atttattata tatgttgttt ggcattgtgt caagcatttt tcatcgatca acccaccagt
     4141 cactgatgtc gtaatatatg ttcaccggat taggtttgac accttgaatt tttttgttta
     4201 tagccaggac tttgttggga ctgtacaaaa acaaatatgg caaatcctcg ttaattatct
     4261 cctgggcttt gtataacaat tccctacggc cttcatggtc ggtagcagct ctagtcaata
     4321 catcgtcaag ctgtggattt gaataggata caaaattgta cccgcttttt atctcactgc
     4381 tggcaaacat gaaacgcaaa tcggggttgc tagccagcat ccatcccatt attgcggcat
     4441 cgtacttttt gcccatgacc cgctgcacca attcatccca ggtgacgttt actaatttaa
     4501 cggaaatgcc gagatccttt aagttctttt ctatgtgaga tgcggtctgg tagcgagcgg
     4561 gattgtcggt gttaactaat agttcaaatt caaatttata ttttttgttc ccgatggttt
     4621 tttctaaaat cccatcatca tcctcatcta tccagcctgc ctcttccagt tgctgtgctg
     4681 ccattttttt gctataagtt acatgtctta tttgcgaatt aaaggcccat gaaaagggca
     4741 aaattggccc gtcaattact atgcccctac ctaaagttgt atcctgcaga atcctatttc
     4801 tgtcaatccc catgagtaag gcctgacgca cattgacgtc gttaaggata ggattggtaa
     4861 ggtttattgc gataaattca aaatatcttg aaggatactg tagtaagtaa acattttcca
     4921 tgtcctcgaa cacattccag tctattggtt caatggccag caagtcaaca tcgccgatgg
     4981 caaatgcttc ttttaaggat gtgctgtcag gatatacctt tatatttatc tcatcgatgt
     5041 acggcacatt gccaaagtaa tcatcatttc tgttcaaagt cactaaattg cttgttctat
     5101 caactacttt aaagggacct gtgcccacca gctcttccat ttcattttct cgggcgtcag
     5161 cttctagaaa acccgcccaa ggtatgcctc ggttcaatcc atacaaaaaa tccgcatcgg
     5221 gcttggatag ggtaataaca agggtgtttt tgtcctgagc ttcatagctt tcaacattgt
     5281 caaattcagt gtatgccggc aaatccttgt tgcctttttg cctttttagc tccataattt
     5341 tgtcaaaaac cgctttcaca tctgcagagg aaaaggtctg gccattgtgc cacgtcacgt
     5401 ctgacctgat ctttatggta taggtcttgc catcatcgct gatctgccaa ctttcagcaa
     5461 gagcgggctg caccatgccc tgggcgtcga ttttcacaag gccttcgaaa atcaggctct
     5521 gaattgccat tgtctcatcg tcatttgcaa ggagtggatt caaatttaat gcacttgtga
     5581 tgcccaaatt tacggtacca ccgctttgag gaggaccggg attttcttgt tcaactgctt
     5641 cattctgttc aacgggattt aaagacgttt ctgaacaagc agtaataaaa aaagtcaata
     5701 ctagaaatag gattattacc tttttagctt tcatgagggc ctcccatcag ttaagcaagt
     5761 ataaatcgga atataaatgt tggtcgtcag gttacataaa taatcccccg acgacctcac
     5821 caattaaatt atatcttagc gattatttgt ggtcaaattc caataattat catgtgtatt
     5881 atcatgttta ttgcgttaaa agcgtttttt tgctaatatg tataaaaaac tttcgaggtg
     5941 aaccatggaa aagactttcg tcatgattaa acctgatgga attaaacgtg gtctcgtagg
     6001 cagaataatt gagcgctatg agcaaaaagg tttaaaaatc tctgcggcaa agctggtaaa
     6061 tgccacaata gacttggctg aaaaacacta cgcggaacac gcatcaaaac cctttttttc
     6121 agagctaata tcttatatca catcaggacc tgttatggcc atggtcctgg aagggcctgg
     6181 tgcaataaaa cttgcccgca taattaatgg tgccactaaa gtggaggatg ctctgcccgg
     6241 cacgatacga ggagactttg cgacatcaac tactgaaaat ctcgtccatg cttcagacag
     6301 ccttgaaagc gcacagcggg aaatcgccct ttggtttcct gaaatagaat aactaattta
     6361 ttttattcta acgagctttg gattgtcctg taatccgggt atccggcccc ttttagcaac
     6421 tggtgttcca tctaccattt ttaaatccat agtcatatca attggagtgg caccggatat
     6481 gtagcttccc acgccaaagg catcagcacc tgcctcaata agtgtcctta tcctttccgg
     6541 agtaagcccc cctgatacaa aaatctttac ataatcatat cctttttgat ttaatcgatt
     6601 tcttatctca tggaccagtc ccggtgttac tccaccccgc tcactaggtg tatcaagcct
     6661 tataccttct aggtcttttc ccaaagcttc tgctacattc agagcttctt ctgcctcatc
     6721 tttaaacgtg tcaaccagaa ttatcctggg agaatcgtca ggcataatct cgttgtaggt
     6781 tttggctaca gaaacggtat ctcctgcaac tatcatggcg gcatgaggaa tggtccctga
     6841 aggttctctg cctaacagtt tggctgctaa aatacagctg gcattatccg caccacctat
     6901 aactgcggct ctttccatga ccggcgctac ggccggatgg acatgcctag caccgaagca
     6961 tataaaaggt ctttgccctg ctgcttcccg acactctctt gcagcagttg cccaagcact
     7021 tggatgtgcc atcataccta aaagaaccgt ctcatatatg ccaaattcct cataggatcc
     7081 cttaatcctc atcaccgttt cttttgcctc aaaggtctcc ccttcgggca gtgcatatat
     7141 ctcaagattc ttgtcatgca aaaggttcag gacctcctgt gtaccgcaca acaccccagg
     7201 ccttctggga aaaacttctg ctacaacttc tttttgggaa aggcccatat ggcgtagaat
     7261 ttccattgtt ttaataaagt atatatccgt agtcatgcct gctaagattt catcgtgatc
     7321 agcagaatgc aaccgtctct tcgaatcaac tttaatgttt tttacatctc ttaatgtttt
     7381 aatttcttcc atccaacaaa cctctctctc aatgtttttg tgtttcatat caatgattac
     7441 tatactatat aattatatcc ataaagccaa taaagtctat aagcctgcca actcacaaat
     7501 tgtacaggcg cctgtaccat ttgtaggacc ttttgactcg cattacgaaa ttcctggtct
     7561 caggaaaagg gatgtcatct tcattttgct gcatcctgcc ttgggccagc catttagcca
     7621 catttccccg acctccgtta tatgccgcaa gggcaaggat cgtattgtta tcaaactcgc
     7681 gcagcagcga ggataagtac caggtcccta tctttatgtt taaatccggg ttttgaagct
     7741 tttctatctg aaaatcttca atccccatct gctgtgccgc ccaaatggca gttttaggca
     7801 taatttgcat aaggccaata gctccctgtt ttgatacgac tttaggggag aagttgctct
     7861 caactttaat gaccgaagca acaaggtatg ggtccacatc atactcttta gcatggctaa
     7921 caattgattg ctcgtatttt agtggatata tgtatctcat aaaccaacct atattgttat
     7981 acagtgctgc aaagccaagg attataacca gtactgctac aagttttttt gacactgtct
     8041 tgatatttcc ctccagatgc tatcgacttg gactttggta tcttcgatgc tgccactatt
     8101 atcgatgacc ctgtcagcat atttaatctt ttcttcttgt ggcatttgag agcgtatcct
     8161 actcatggct tgttccttat ttagattttt atccctattc atcaacctct ccagctggac
     8221 tttttcgtca acggtcacaa cccaaacctc gtcaacgatt gcatctaacc ccacttccag
     8281 gagtagtgct gcatcgataa taacaacctc tttttgcaag cttttataat actccagctg
     8341 agattttatt tcatttatta tttcgggatg cgtaaagctg ttgagaaatt caagctgtct
     8401 tttatctgaa aagacaatat ctgccagttt ctttctgtcg atatactcct cttcatttaa
     8461 aatctcatgt ccaaaatggt taataacttt aaaccatgcg ggtttgtccg gctgcattat
     8521 ttgcctcgct atctcatcag catctattat aacagcacct ttgtccttaa gcaataagga
     8581 caccgtgctc ttgccgctgg caatcccacc tgtgagccca actacaaaca ataggtaccc
     8641 ccctcataat tcttcccaag tatatccctg agtaaagtca actacaaggg gaactcttaa
     8701 gggaatggca ttttccatgt ctgtttttaa tatttctttt acgatggaaa gttcatcttc
     8761 cggtacatcc aatatcagtt catcatgaac ctgcaatata attttagctt ttaagttttt
     8821 ctctcttaaa tgtctgtaca cctttaccat ggcaaccttg atgatatccg cggcactacc
     8881 ctgaatgggt gtgttcatgg cagtccgctc cgcaaaagag cggagatggt aatttctgct
     8941 atttatatca ggtatatagc ggcggcgatt tagcaatgtt gttacgtaac ctgaaagttt
     9001 tgctaatcgt atagtgtccc ttacatagtc tctgacaccg ggatatcgct cgaaatatgc
     9061 atctatgtat tctttagcct cctggttgga tatgcctaat ccttgagcca ggccatagtc
     9121 gcttattccg taaacaattc caaaatttac agctttcgcc ttgtctctaa gggaaggtgt
     9181 cacatcttcc ggcgaaactc caaaaacttc actggccgtt cgggtgtgta tgtcctctcc
     9241 cttaataaag gcttccatca aggtcctatc ttcagagatg tgggccaata ccctaagttc
     9301 tatctgggaa taatcacctg acaaaagtac atgtcccgga ccgtccgctg taaatacccc
     9361 cctgatatgc ctgccttctt cggttttcac gggtatgttt tgcaaattag gttccgtgct
     9421 gctgatacgc ccggttgatg taacagtttg attaaaattg gtgtatattc tttctgtcac
     9481 cttatcaact aaggctaaaa ggccatcagc ataggtagat ttcattttca ttaaaaacct
     9541 gtactccaaa atgctttcaa caattgggtg actgcctctc agtttttcca ggacctccgc
     9601 atctgttgaa aagccagtct tggttttctt aatcacaggc aaggacaatt tctcaaatag
     9661 cacttcaccc aattgtttcg gtgagttgat attgaattcc atgcccgcct tactgtatat
     9721 atcttctgta agaatgtcga gtttttcgcc aaaccttgca cccagttcct tcagcttttc
     9781 ggggttcacc tttatgccga tttgttccat gtctgccagc actacgctta agggcatctc
     9841 cacctcgtaa aagagccttt gcatttcaaa atcgcgtatt ttgtcatcta ggaccttctt
     9901 taaactctta agatatgcgg ctttttcacc taagtcggca gcttcatcga gcttttcccc
     9961 taagtattcc agtgccaggg tagcaatgtc ataattaggt tttgaaggct ccaaaaggta
    10021 agctgccagc ataacatcga attgacttgc aaagtctata tcgagctgct tgagaacctt
    10081 tctcaggaat ttgccatcat gtgtaatttt atatacgtca tcatcttcta aaatggattt
    10141 tagctgcctt ataagattct tatcatctag aagtgaaaca tctatgtagt aacttttgtc
    10201 tttcaatgaa atatagatgc cgtttatcgt aaaggtcttt aagtctttgg cctttgcatc
    10261 cacaagtact ccaattagtt tttccaaact aatctctgag accaagtttt gaaaatcatc
    10321 ggcattattg atttttattg ttttaaatga ttcttgtttg tcattattgt ccgtaggctt
    10381 tatcctatcg atcaaactgt aaaactccag ttcccgaaat aaggtaatta acctatcata
    10441 gtcgggttca cggctggcta aatcctcaaa actaaagtct acggatacat ccgttacaat
    10501 tgttgccaag tatttgctga ttttagcttg ttcatgatat acttcaagat tttctttgag
    10561 cttgcccttc agcttttcag tatttgctaa aatattatct atcgtgtcaa aatcacggag
    10621 caattttgcc gctgtctttt ctcctatacc gggcaccccg ggaatgttgt cggacttatc
    10681 accaacaagg cctttcatat ctactatgcg ttcaggggga atgccgtacc tttctttcac
    10741 ctttgccggg tcgtaaacct ccaactctga aatgcctttt ttggtaagca tgacactggt
    10801 atttgaagac acaagctgca gggcatcttt atcgccggtc actatgacag aaaaaactcc
    10861 ggcctcttcc ccttttttgg acatggtccc caagatgtca tcggcttcaa agccgtcaac
    10921 ttcgaagtaa tgtatgttga gagcttttaa tatgtccttt agtatgtcaa actgcccggc
    10981 tagttcctca ggggtctttg gccgcgttgc cttgtatgct acgtactcat catgcctaaa
    11041 agtcgggcct tttttgtcaa aagcaacgga aatgtaatca ggtttttcct cctgtaaaat
    11101 tctaaacaac atattcagaa aaccgtatac agcattggta tgtaccccct tggaggttct
    11161 taaggggggc agtgcatgaa atgccctgtg aagtaggctg tttccgtcta ttaatagcat
    11221 ctttctcata ttcttcaact cccgtattac tattatttca ccaaaaatct ttaaaaacct
    11281 gcaaaatatc aaaaaagtta gttctaaatc tgtagggatt tagaactaac ttttaatccc
    11341 tttgttgtgt attaaattgg agctaacctt ttttcctttt gctcaaaaca cgcctgatta
    11401 gcaagtatag aataccgaga attagcgcta ctggcaaaag ggcaccagtg agttctgcta
    11461 aaaacgccaa catattcatt gaaatgttta tggcggtgat aaagccttgt cttgcccttt
    11521 gcatcaattc caggggagta acagttgtca ctttttcttt tgatggctcc tgttcaatca
    11581 aattaacatt gactaaagac atttctacca ttttgtccca tgccatcagt tgtcctttaa
    11641 gcatgtctat atcagttctt accctgttta gctcaatttc gattcgaagt atttcatcaa
    11701 tagtattcgc catgtttaat atttccagga gcctctgctc ttgctgaact aggttccgga
    11761 tcctcgcatc cgtgtcatag tatgcatcag taatattctc accacttatt tggttattgg
    11821 tgactcttcc aagggtctta aatatgtcca gtgcatcatt gagcttagcc tgtggaattc
    11881 tcaaagccat atatgctcta cggggggaat ctatcgactt tcctccctgt atttggctgt
    11941 gctctatata accacccaac tcttccacgg tttttattat ataatcgacc ttttcatcga
    12001 atttttcaac gtatattgat agatcggcat ttttgatgat cttctggctt ttttcctcgg
    12061 cagaaaaggt tttgaggcga gtatctgcca tgtttgtcga cgggacttgg gcaatgcttt
    12121 ccccttctga ttgcatccaa cctgatgtcc cagctccgtc cattgtcatc atggaagaca
    12181 tgtcggcagg tgcggattca gaaactgcct cttcttttac actattggtg gcatagttca
    12241 tatcaaaaac accttggttg tacgccgtaa ccacaaatat tccgatgagg aatacgctaa
    12301 gggcaccaac aaaaatcctc ttattatgat aaatataagc atataataat tccgggatat
    12361 tccttattag ctttaacagt ggcagcgatt tttcatggga cctttgttga taagacaggg
    12421 tattgatctt tcccattaaa ttagccttta aatcaaccgg taactcaact tgtcccaggg
    12481 aagctgaagc tttgataatt ttctggagct cttttgcttc ctgtctacaa tcgggacaaa
    12541 cttccatgtg gctttcgagt aattcttttt cttcaagggt taccaggcct tcaatgtatt
    12601 tgtcaatcaa atcttcatag tgtttgctgc atcccacgtc gttcccccct ttgtactaat
    12661 atagacgtca gttgctcaaa aaaagttccg ggctcttgag tttttccctt aatgcccgtc
    12721 tgcccctact cagtctggat tttatagtgc caagggaaca catcaaaatt tcactcatct
    12781 cggtataact gaagccttga atatccctta atattacgca tatgcgctgc tctttcggaa
    12841 gggtatttat cgcttttaaa agaacttctt tcagctcctg tttttccaca aaatcttcag
    12901 gtaactgtgt ataagaactg tgctttaaat cgtttcctag tggcatttcg tcttttatgg
    12961 ggagggtttc atccttcttt ttgcgcaaaa aatcaatgca ggtatttacc gcaatgcgat
    13021 aaatccaagt agaaaagctg gaatcctctc taaagctgtg gagggaggta taaactttta
    13081 taaatatttc ctgggtaata tccatagcat cttctgaatt attgaaaaaa cggtaaaccg
    13141 tattgtacac ttttttctca tatccggcaa tcaattcttc aaaggctgaa acatcgcctt
    13201 ttttggcccg gcttatgagc tctttctctg cgttcaaaat tatttccccc cttcacttta
    13261 ctctcctagg tattcgactt taaggcttaa aattccttca aaaagaatag ccgaaattta
    13321 ccgtcggtat attttaagca agcctcaaac ctaaactctt tgcataatat aaggcttcca
    13381 tatattcccg gttactgagc cttcttgata tttcctcata ttggtgggct ttatttgccg
    13441 gataatattg atcgtccatc aggttaacca agcagtcagc tgacaattcc tcctttatga
    13501 atttcaaaac gtcttttgtg tcttcaagac cgccgggcat aaccaggtgc cttatgagca
    13561 agcccttgta agctatgccc ctctcatcgg tttttaaacc tctcacctga cggtccattt
    13621 cctttaaagc cagctttgct ttctggggat aatcctttac tccagagtac ttccggccac
    13681 gttcagaaaa cgcatatttg aaatcaggca tgtaaatgtc tatgacacct tctagtattt
    13741 taagggtttc tattttttca tagcctccgc aattatagat caagggcagt tttaagccct
    13801 tttgagctgc aatatataaa gcctctagaa tattgggtac aaagtgggtg ggggtgacga
    13861 ggttgatatt gtgacatttg taatggtttt ggagcagaag cataatttct gcaagctttt
    13921 catccgaaac ggctgccccg tagccacgaa agctgagttc atagttctgg caaaaaacgc
    13981 accgcatgtt acaatagccg aaaaatatgg tgcccgaacc atttctgcca actaaaggcc
    14041 tttcttcgcc gaaatggggg ccgtaacttg caacaatggc catttcccct gctcggcaaa
    14101 ctcccagatt ctcctttctg ttcacattgc attcatgagg acacaaattg catttgctta
    14161 aatgctgttt ggcttgtctt accttttcaa gcaattggcc attccccagt aagctaagat
    14221 agcccggcaa catgtttttt gccatgttct ttttcccctt tttaacattg tatcgataat
    14281 attcttccat tcatatattt cttaattgta ttcactgtat gtgtttgtga aataatttta
    14341 ccatatctct tcaagcttga aatttagaaa gcaacgcctc atttatactt gcttaggaaa
    14401 gcgggtacag attgtcaaga tgcctcttat attgagacat attataaaat gtttcagaca
    14461 agtctatcat caaactccgt aatatcaccg gtttgcgttt taaaaacatc cgccatctca
    14521 tcattccgat ctcgcttatc ttggctgaga acgtttcttg tattgtagca tcgctcgcat
    14581 agactgctgg gatgtacata aacatagtct cattatagca tggttggtaa ataatttttg
    14641 atatgtttaa atatcattaa gagtacgatt attttaatta atgcaaaaat aaaatcttgg
    14701 aaggaatcat caagtgaagc tttttgacaa acttcccccc aatctttttt ctatactggt
    14761 ttccaaaaac aaggcactgt acgctgaggc tttgtttgtc ctgcgtaaag cattcaagca
    14821 gcata
//
LOCUS       DAONUO010000007        13890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_3359_length_13890_cov_4.201879, whole genome shotgun sequence.
ACCESSION   DAONUO010000007 DAONUO010000000
VERSION     DAONUO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_13890_cov_4.201879"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1267
                     /gene="nifS"
                     /locus_tag="PK768_00585"
     CDS             77..1267
                     /gene="nifS"
                     /locus_tag="PK768_00585"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HQA59499.1"
                     /translation="MNRIYMDHAGTTPMRSEVLEAMLPYLTEKFGNASTIYSYGREAK
                     KALEDSREKVAQLIGANTKEIYFTSGGTESDNWALRGVAAANIKKGKHIITSAIEHHA
                     VLHTCQALERQGFKITFLPVDEHGLVNVEDVVNAITDETIMVSIMHANNEIGTIQPIK
                     EIGKAIKREKKDIVFHTDAVQTVGKIPVDVDDLGVDLLSISAHKIYGPKGVGALYIRK
                     GTKILQFMTGGAQEGNRRAGTENIPGIVGFGKAAELAKTELKEQYDKLIVLRDKLIKG
                     IMDNIPYTRLNGHPALRLPHNVNVSFEFIEGESMLLNLDMKGICASSGSACTSGSLDP
                     SHVLLAIGLPHEIAHGSLRLTLGRDNTEQDVDYVLEVLPGIVNKLREMSPLFAEGKEQ
                     AKNV"
     gene            1260..1640
                     /gene="nifU"
                     /locus_tag="PK768_00590"
     CDS             1260..1640
                     /gene="nifU"
                     /locus_tag="PK768_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HQA59500.1"
                     /translation="MYNEKVMDHFANPRNVGEIPDADGVGTVGNAVCGDIMQMYIKVK
                     NDIIEDIKFKTFGCGAAIATSSMATEMVKGKSIDEALKLSNKQIAEALGGLPPVKMHC
                     SVLAADALREAINDYKKKVGSNIG"
     gene            1633..2724
                     /gene="mnmA"
                     /locus_tag="PK768_00595"
     CDS             1633..2724
                     /gene="mnmA"
                     /locus_tag="PK768_00595"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQA59501.1"
                     /translation="MDKNKVVVAMSGGVDSSVCAYLLKQQGYEVVGMTMQIWQDLPYE
                     DQLKEGGCCSIGAVHDARRVADKLDIPYYVINLKDSFNEKVIKHFVQEYLSGKTPNPC
                     IACNKHLKFEELLRKAFDIDAFYLATGHYAKVEYDNVRKRYVLKKAVDNDKDQSYALY
                     NLNQFQLEHTLFPLGYYTKKEIRKIAEEIGLAVADKPDSQEICFVNTNYRDFIREKSP
                     ESIKEGYFVDIKGNILGRHKGIPFYTIGQRRGLGISAGKPLYVIDIDVNNNKIVLGEE
                     KDLYVKEFLAHKLNWVAIDSLNEELELMAKIRYNFHEKAAVIKPHGNDMVKVVFDEAQ
                     KAVTPGQAVVFYQDDLVIGGGIIKQRCDL"
     gene            3056..4042
                     /locus_tag="PK768_00600"
     CDS             3056..4042
                     /locus_tag="PK768_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQA59502.1"
                     /translation="MFIAIILLLLFIINNREKIAQVLQPFVFGVVIAYILNPVVEILT
                     ENKMHRTAAVALIYFILIGSMIIALVYILPVIIIELNNLMDTLPLYSREVQKFLASIK
                     TSYLAALPASLQEIVDRNISRMEELLLRYLQKIADGIIIVLSSILSIILGPILGFYFL
                     KDLDKIKESAFLYIPAPNREIVKHWVERIGITLGRYLRSQLLVSLIVGILTTLSMLIL
                     NIDFAILIGVVAGLANIIPYFGPIIGTMPAIAIAILRYPNRIPWIIILMLVIHQLESG
                     IISPHIVGEYVGLHPITVIFVLLTGGMFFGLTGLILAVPVAALIKLIMQSKK"
     gene            4193..4894
                     /gene="rpsB"
                     /locus_tag="PK768_00605"
     CDS             4193..4894
                     /gene="rpsB"
                     /locus_tag="PK768_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQA59503.1"
                     /translation="MSIVSMKQLLEAGVHFGHQTRRWNPKMKEYIFTERNGIYIIDLQ
                     KTVKKIEEAYEFVKNISADGGNILFVGTKKQAQESIQEEAQRCGMFYVNQRWLGGMLT
                     NFKTIRKRIDRLNELEKMEEEGLFEVLPKKEVLNLQHEKGRLEKNLGGIKDMTDLPEA
                     VFIVDPRKERIAVQEARKLDIPIIAIVDTNCDPDEIDYVIPGNDDAIRAVKLLTEKIA
                     DAVIEGRQGEQMEEE"
     gene            4958..5551
                     /gene="tsf"
                     /locus_tag="PK768_00610"
     CDS             4958..5551
                     /gene="tsf"
                     /locus_tag="PK768_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQA59504.1"
                     /translation="MVTPEQVKELRGKTGAGVMDCKKALVEANGDMEKAIVILREKGL
                     AKAAKKQSRSATEGIIESYIHGNGRIGVLVEVNCETDFVARNEEFKILAKDIAMQVAA
                     SNPKYLSREDVPDEVIEKEREILRAQALNEGKPEKIVDKIVDGRIEKFFEENCLLEQA
                     FIRDPDKKVSQYIMEKIAVIGENITVSRFVRFERGEV"
     gene            5661..6374
                     /gene="pyrH"
                     /locus_tag="PK768_00615"
     CDS             5661..6374
                     /gene="pyrH"
                     /locus_tag="PK768_00615"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQA59505.1"
                     /translation="MKKPVYKRVVLKISGEALAGDKGFGIDQDILNSIAGQISEIHNL
                     GVQVAIVVGGGNIWRGRNSKQMDRATADYMGMLATVINALALQDALENRGVQTRVQTA
                     IEMREVAEPYIRRRAIRHLEKGRVVIFAAGTGNPFFSTDTTAALRAAEIEAEVILLAK
                     KVDGVYDSDPVLNESAQKFDSLDYIEVLNKGLGVMDSTATSLCMDNSIPIIVFGISTP
                     GNIKKVILGENIGTLVRGS"
     gene            6381..6938
                     /gene="frr"
                     /locus_tag="PK768_00620"
     CDS             6381..6938
                     /gene="frr"
                     /locus_tag="PK768_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQA59506.1"
                     /translation="MDKKLFSEMEKRMDKVLAILKSDYATLRTGRASTALLDKIYVDY
                     YGTPTPINQVASLSIPEPKMILIQPWEVKMLQTIEKAILKSELGITPNNDGKVIRLIL
                     PELTAERRQELAKFAKKKCEEAKVAIRNIRREYNDALKKMEKNKEISEDELKRSQEEI
                     QKITDKFIDEADKILANKEKEIMEV"
     gene            7269..8012
                     /locus_tag="PK768_00625"
     CDS             7269..8012
                     /locus_tag="PK768_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQA59507.1"
                     /translation="MGHAKAAIDKINVKNLPKHIAIIMDGNGRWAKQKGLPRIAGHWA
                     GAEALRDIVRTCCKLNIQVLSVFAFSSENWKRPVQEVNTIMNLLLYYLKKEVDELDKN
                     NVRIHVSGAWEELPERIVGQIKKSIQQTQNNTGLILNIVLNYGSRNEILSACKKLYKD
                     VQSDIISIDGIDESTFQNYLYTRDLPDPDLLIRPSGELRISNFLLWQIAYTELWFTEV
                     YWPDFKSDDLLKAILDFQKRERRYGGLKS"
     gene            8026..8835
                     /locus_tag="PK768_00630"
     CDS             8026..8835
                     /locus_tag="PK768_00630"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQA59508.1"
                     /translation="MITRIISAVVGIILLGYVINTGGWVFYAAISFLNICALYELRSA
                     FSKKNIHIRFFLNSALAVVLFYCASFKNFDFILTYLFAILLLMFEFLYNIIVSNENHL
                     KDMVFSVFSFLYTSFLFMSIILVRNISLGLKLTWWIFITTWACDTGAFFSGMIIGKTA
                     LAPAVSPNKTIEGSLGGILSSVAASVLYSVYFLPEINTLHAILFGICIGVVAQIGDLS
                     ASFIKRYCQIKDFSNIIPGHGGILDRLDSALFSFPVAYIFIKLLLQKGGLL"
     gene            8832..9911
                     /gene="ytvI"
                     /locus_tag="PK768_00635"
     CDS             8832..9911
                     /gene="ytvI"
                     /locus_tag="PK768_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HQA59509.1"
                     /translation="MKMRTTYSPIFFLVLAVAVTAIVIVLAIKYFLPLVIGIFIAILI
                     DPIVTYLERKLELDRGIITVIVLSAIFCLTGYISLLIIARFTFELARFTNFLPTQLRH
                     FNALIDKSYLYLSNFFAKVPEDIIVYLKANLNQILSTISGSLSNLYTFLVNKIGMVPN
                     LLVNLLVVIIFVFLFSYFLTKDKDKIIHIIKNLFPEQLHEKVKSVQLELVFSFFRLIK
                     AQIILVLISTVITVLGFCILRVEYALTLGIICGILDIMPLFGPSLIFIPWIIYLLVIG
                     NVNFAIGLLFLYVDVIGSRQMFQAKIIGRNLGIDPLLTLLSIYLGVKVFGWKGLFVGP
                     LVVVIVRALIHCGIIPPLNKPTKKV"
     gene            9932..10933
                     /gene="rseP"
                     /locus_tag="PK768_00640"
     CDS             9932..10933
                     /gene="rseP"
                     /locus_tag="PK768_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQA59510.1"
                     /translation="MQTILSSILVFGMLIFFHELGHFIVAKLSDIKVNEFSLGLGPQL
                     ISMKLKETHYSIRLLPFGGYVKMEGEDTDTSDPRAFNNKSALVRLGVILAGPLMNFIL
                     AILLLGIIGFYSGVPTRQVTVTPGEPAEISGIQDNDIIHAIDGRKVGHWEEIVEIISG
                     RYNEKISVTVIRDGKYIDYDVTTTVEPKTQRGIIGIKSVMQKHSVLESIKYGYKKTVL
                     ILKLILVGIYQVLTGKVKADVVGPVGLAHIVGEAAKIGVYQVLYIAAVISANLGLFNL
                     FPIPALDGGRGIFLVFELLRGKPVEPEKEGLIHFIGFALLMFLMILILFKDLRELWF"
     gene            11003..12079
                     /gene="ispG"
                     /locus_tag="PK768_00645"
                     /gene_synonym="gcpE"
     CDS             11003..12079
                     /gene="ispG"
                     /locus_tag="PK768_00645"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQA59511.1"
                     /translation="MLEFSTIKRRKSRKVMIKDVGVGGDSPITVQSMTNTKTDDIEST
                     VNQIISLENAGCDIIRVAVPDMASAEAISKIKEQIHIPIVADVHFDYRLAIEAIKQGA
                     DGLRINPGNIGDAEIIKKVVETAKSFDVPIRIGVNSGSLEKDLLEKYGGPSPEALVES
                     ALRHVEILENMNFEHIVISLKSSDVLSCIHSYKLIAQKVDYPLHLGITEAGTLLTGTI
                     KSSVGLGILLYEGIGDTIRVSLTGEPVDEVKVGFEILKSLNLRQYGINFISCPTCGRT
                     QINLIKIANEVQEKLSHIKKPLKVAIMGCIVNGPGEAREADIGIAGGKKRVAIFKKGE
                     IIKTVSEEDAVSELVKEIKHMINE"
     gene            12107..13543
                     /gene="proS"
                     /locus_tag="PK768_00650"
     CDS             12107..13543
                     /gene="proS"
                     /locus_tag="PK768_00650"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQA59512.1"
                     /translation="MENDFVKEITPKSEDFSQWYVDVILKAELADYSPVRGCMVIRPY
                     GYSIWEKMQQLLDSRFKATGHKNAYFPLFIPESLLQKEAEHVEGFAPEVAWVTHGGNE
                     ELVERLAVRPTSETIICHMYSKWIKSWRDLPVLINQWCNVVRWEKSTKPFLRTAEFLW
                     QEGHTAHSSFEEAEEETLRMLEVYRDFVETDLSMPVITGVKSENEKFAGALKTYTIEA
                     LMGDGRALQAGTSHNLGQHFAKAYDITYLDKDGKLKHVWQTSWGVTTRLIGAIIMVHG
                     DDRGLKLPPKIAPIQAVIVPILGKESETVLNKARELYSILNKEIKTELDDRNEYTPGW
                     KFNEWEMKGVPVRIEIGPRDIKNEQVVFVRRDTGEKITVSESELLPRLKSLLDEIQAN
                     MFRQAQVFLNENIREVDNFDEFKQIIDEKRGFIKAHWCGNTECEKKVKEETGATLRCI
                     PFCKETISGKCIVCDKQSDTVAYFAKSY"
     gene            13680..>13890
                     /locus_tag="PK768_00655"
     CDS             13680..>13890
                     /locus_tag="PK768_00655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA59513.1"
                     /translation="MPKIAVVIPAYDEEKTIGNVLKPVSAISMIDDIIVVSDGSHDRT
                     ANIARSYKARVIEFDKNHGKTYAVLS"
BASE COUNT         4655 a         2125 c         3065 g         4045 t
ORIGIN      
        1 aataatagcg aaacttaaag attcagagag tttgtttatt ataaacgaga ctatattaaa
       61 aaggagatgg caacttatga atagaatata tatggatcat gccgggacta cacccatgag
      121 gtctgaagtt ttagaggcaa tgttaccata tcttactgag aagttcggaa atgcttctac
      181 aatatattca tatggcagag aagctaagaa agcccttgaa gatagcaggg aaaaggtggc
      241 acagctaatc ggtgccaaca caaaagagat atattttact tccggaggga cggagtctga
      301 taattgggca ttaaggggag ttgcggctgc caatataaaa aagggcaaac atattataac
      361 ttcggcaatc gaacaccatg ccgtattgca cacatgtcaa gccttggaaa gacaggggtt
      421 taaaattact tttttacctg tggacgaaca tggtttagtt aatgtagagg atgtagtcaa
      481 tgcaattaca gatgaaacta tcatggtttc gattatgcat gctaacaacg aaatcggaac
      541 aattcagcca ataaaagaaa ttggcaaggc cataaaaagg gaaaaaaagg atattgtttt
      601 tcatactgat gccgttcaaa ctgttgggaa aattcctgta gatgttgatg acttaggtgt
      661 tgaccttttg agcatatctg cacataaaat ctacggtcca aaaggtgtag gtgcccttta
      721 catcaggaag ggtaccaaga tattgcaatt tatgacaggt ggagctcaag aggggaatcg
      781 cagagcagga actgaaaata ttccgggaat tgttggattt ggaaaagcag ctgaacttgc
      841 caaaactgaa cttaaggagc aatatgataa gcttattgtg ctgcgagaca aattgataaa
      901 aggtataatg gataacatac catacactcg gctaaacggt cacccagcct tgagattgcc
      961 tcataatgtg aatgtatcat ttgaatttat tgagggtgaa tcaatgctgc tcaatttgga
     1021 catgaagggt atttgcgcat ccagcggttc agcttgcacg tcgggttcgt tggatccttc
     1081 acacgtgctg ctggctattg gcctgcccca tgaaattgca catggatcat tgcgtttaac
     1141 actgggcaga gacaataccg aacaggatgt agattacgtt cttgaggtgc tgcccggtat
     1201 agtaaataaa ttgagagaaa tgtcaccttt atttgcagaa ggaaaggagc aagctaaaaa
     1261 tgtataatga gaaggtaatg gatcactttg caaatccacg gaatgttgga gaaattccag
     1321 atgctgacgg agttggaaca gttggcaatg cagtttgcgg agacatcatg caaatgtata
     1381 taaaagtaaa aaacgatatt attgaggata tcaaatttaa aacttttggc tgtggcgctg
     1441 ccattgccac aagtagcatg gctactgaaa tggtcaaggg taaatctatc gatgaggcgt
     1501 taaagctaag caataagcag atagctgaag ccctgggagg gctgcctcct gtaaaaatgc
     1561 actgttcagt tctggccgca gatgcactaa gagaagcgat aaatgattat aagaaaaaag
     1621 tagggagcaa tattggataa aaacaaagtt gttgtcgcga tgagcggagg agtggatagc
     1681 tccgtttgtg cttatctgtt gaaacagcag ggatatgaag ttgttggtat gacaatgcag
     1741 atttggcagg acttgccgta tgaagatcag cttaaagagg gtggatgttg ttccattgga
     1801 gctgtacatg atgcacgtag ggtagcagat aagctagaca ttccatatta tgtaatcaat
     1861 ttaaaagatt cttttaatga aaaagtaata aaacactttg ttcaagagta tctatctgga
     1921 aaaactccaa atccatgcat tgcctgcaac aagcatttaa agtttgagga attgcttaga
     1981 aaagcatttg atatagatgc cttttacttg gcaacgggtc attatgccaa agtggaatat
     2041 gacaatgtca gaaagcggta tgttcttaaa aaagctgtcg acaacgataa ggaccagtct
     2101 tatgctcttt acaatctaaa tcaatttcaa ttagaacata ccctttttcc actgggctac
     2161 tatacaaaaa aagaaattcg taaaatagct gaagaaatag gattggccgt ggctgataag
     2221 cctgacagtc aagaaatctg ctttgtaaat accaactatc gtgactttat acgtgaaaaa
     2281 tctcctgaaa gtatcaaaga gggttacttc gtagacatta aaggaaatat attaggcagg
     2341 cataagggta ttccctttta tactataggt caaagacggg gacttggaat ttcagcaggc
     2401 aaacccctgt acgtaatcga catagatgtt aataataata agatagttct aggcgaagaa
     2461 aaagatttat atgtaaagga atttttggca cataaattga actgggtcgc aatagattcc
     2521 ctgaacgaag agcttgagct aatggcgaaa atcagatata attttcacga aaaagctgct
     2581 gtgattaaac ctcatggaaa tgacatggta aaggtagttt ttgatgaagc tcaaaaggct
     2641 gttacaccgg ggcaagcagt agtcttttat caggatgacc ttgtaattgg cgggggaatt
     2701 ataaagcaaa ggtgtgattt gtaaaaagat acgcttaaat gcgtattttt ttatttactg
     2761 ggaaaaatat taatatcaac aattaaatat tcatgagtag aggtgaaaat cttggtagcg
     2821 tgtcctttgt gtcaaaaaaa cactgtaggc aaggttggaa caaatcaata ctattgttgg
     2881 gaatgttttg ttgaattttc aattcaaaaa gataaaacta tgatatatga gctaagcgac
     2941 gatggcagct tactgccata cggagaaaac caacaattaa ctatgggaaa ttgaagagag
     3001 gagtgacata attattaaat tgttttcaaa ccggatattc ttataccggc tttttttgtt
     3061 tattgcaatt atattattac tgctttttat aattaacaat agagaaaaga tagcccaagt
     3121 tttgcagcca tttgtttttg gagttgttat cgcttatata ttaaacccgg tcgttgaaat
     3181 cctaacagaa aataaaatgc accgaacagc tgctgttgct ttaatatact ttattttgat
     3241 aggatcaatg attattgctc ttgtttatat attaccggta ataattatag aattaaacaa
     3301 tttaatggac acacttcctc tttatagcag ggaagtgcag aaattcttgg caagtataaa
     3361 aacaagctat cttgcagctt tgccggctag tcttcaagaa attgtggata gaaacataag
     3421 tagaatggaa gagctattat tacgctattt acaaaaaata gcagatggga ttattatagt
     3481 gttatcaagt attttaagca ttatcttagg ccccatacta ggtttttatt ttttaaaaga
     3541 cttagataaa ataaaggaaa gtgcattcct ttacatacct gctcctaata gagaaatcgt
     3601 gaaacattgg gtagaaagaa tcggcatcac tttaggcaga tatttgagaa gtcaattatt
     3661 ggtcagtctt atcgtgggca ttctaacaac actttcaatg cttattctaa atatagattt
     3721 tgctatactt attggtgtgg ttgcaggttt agcaaatatt ataccgtatt ttggccctat
     3781 aataggaaca atgccggcca ttgctatagc tatccttcgc tatcctaata gaataccttg
     3841 gattattatt ttgatgttag taatacacca gttggaaagc ggaataatct cacctcatat
     3901 cgtaggggaa tatgttggcc ttcatccaat tacagtaata tttgtacttt taacgggtgg
     3961 aatgtttttt gggttaactg ggctaatctt agcagttccg gtagcagcac taattaaact
     4021 aattatgcaa agcaaaaaat aaaaatgttg ttggaagttc ttgcactaac cttgttttta
     4081 tggtatacta tcaaccggca aaatacacac gctgatggat tttgaagagg gtgccgtaag
     4141 gtctcttcaa tcaatgaaat cagcggaggt tttaacatta ggaggtgaaa aaatgtcaat
     4201 cgtctcaatg aagcaattgc tagaagccgg tgtgcatttt ggtcatcaga caagaagatg
     4261 gaatcccaag atgaaagaat atatttttac agaacgcaat gggatatata ttatcgacct
     4321 gcaaaaaaca gtcaagaaaa tagaagaagc ttacgaattt gtcaaaaata tatctgctga
     4381 tggcggtaat atactttttg tcgggaccaa aaaacaagcc caggaatcaa ttcaggaaga
     4441 agcccaaagg tgtggcatgt tttatgtaaa tcagcgttgg ctgggcggta tgctcacaaa
     4501 ctttaagaca atacgaaaga gaatagaccg gctaaatgag cttgaaaaaa tggaggaaga
     4561 aggtcttttt gaagtattgc ccaaaaaaga agtgttaaac ttgcagcatg aaaagggtcg
     4621 tttggagaaa aacttgggcg gtataaaaga tatgacagat cttccagaag cagtattcat
     4681 cgtggatccg agaaaagaac gaattgctgt tcaggaggct aggaaattag atattcctat
     4741 aattgcaatt gttgatacaa actgtgatcc ggatgaaata gattatgtca tacccggaaa
     4801 tgacgatgct atcagagcag ttaaattgtt aacagaaaaa attgccgatg ctgtcattga
     4861 aggtaggcaa ggcgagcaga tggaagaaga atagtataaa caaggtaagg gggatttgct
     4921 acccttacct tttatttcaa aacagggagg ttttattgtg gttactccag agcaagttaa
     4981 agagctaaga ggaaaaacag gtgccggcgt tatggattgt aaaaaagcct tagtcgaagc
     5041 caatggagat atggaaaaag caatcgttat actaagagaa aagggattag caaaagcagc
     5101 gaaaaaacaa agtcgctctg caactgaagg gataatagaa tcttatatac acgggaacgg
     5161 ccgcataggt gttttagttg aggtaaattg tgaaacagat tttgtagcta gaaatgagga
     5221 gtttaagatt ttagcaaagg acattgcaat gcaggtagct gcatcaaatc ccaaatattt
     5281 atcccgcgag gacgttcctg atgaagtcat tgaaaaagaa agagaaatac tgagagctca
     5341 agctctaaac gaaggcaagc ctgaaaaaat agtggacaag attgtagatg gacgtattga
     5401 gaaattcttc gaggaaaatt gccttctaga acaggctttt attagggatc cggacaagaa
     5461 agtttcacag tatataatgg aaaaaattgc tgtaattggt gaaaatatca ccgttagtcg
     5521 ttttgtacga tttgagcgtg gagaagttta ggcatttcta tttaatacta taaaaaagag
     5581 acaccataca agtgtctctt ttttaaaaaa agaggatttt ttgagaagac atcgaattcc
     5641 tttagaaaag aggactgtgg atgaaaaaac cggtttataa aagagtcgta ttaaaaatta
     5701 gcggtgaagc cttagctggt gataagggct ttggcatcga ccaagacatt ttaaattcca
     5761 tagctggtca gattagtgaa atacacaacc tgggtgtgca agtagccata gttgtaggtg
     5821 gtgggaatat atggcgtggt agaaattcga aacaaatgga ccgtgcaact gccgattata
     5881 tgggaatgct tgctaccgta attaatgcac ttgccctaca ggacgcattg gaaaacagag
     5941 gagttcagac aagagttcag acagctatag agatgagaga agtagcagag ccgtatatca
     6001 ggagaagagc tataaggcac ttagaaaaag gtagagttgt catttttgca gcaggcaccg
     6061 gaaatccatt cttttctacg gataccacag cagctctgag ggcagctgaa atagaggcag
     6121 aagttatatt gcttgcaaaa aaagtggacg gtgtttatga ttctgacccg gttttaaatg
     6181 aatctgcaca aaaattcgac tctcttgatt acatcgaagt tctaaataaa ggcttaggag
     6241 tgatggattc tacggcaaca tcattgtgca tggacaacag tattcccatt attgtctttg
     6301 gcatttctac acctggcaat ataaaaaagg taatattagg tgaaaatata ggaacattag
     6361 taagggggag ttgatgagaa gtggacaaga agctattttc cgaaatggaa aaacgaatgg
     6421 ataaggtttt agccattcta aaatcagatt atgctacttt aagaactggg agagcttcca
     6481 cagcattact agataaaata tatgtagatt actacggtac accgaccccg ataaatcaag
     6541 ttgcctcact tagtattccg gagcccaaaa tgattctgat acagccatgg gaagtaaaaa
     6601 tgttgcagac gatagaaaag gcaattttga aatcggagct aggcattact ccaaataatg
     6661 acggaaaagt aattagatta atactaccgg aactgactgc tgaaaggcgt caggaacttg
     6721 ctaaatttgc caagaaaaaa tgtgaagaag ccaaagttgc aattagaaat atccgaagag
     6781 aatacaacga tgctttaaaa aagatggaaa aaaataaaga aatttcagaa gatgaattga
     6841 agcgttcaca ggaagaaatt caaaagataa cggacaaatt catagatgaa gctgataaaa
     6901 tcctagcgaa caaagaaaaa gaaataatgg aggtataaca atatttaaaa ataacctcaa
     6961 gttgattgct atgcctttag ataaagccac taaattatta ttaaatcggg gaatccatcc
     7021 gaaaattatt tatacgagag cccctaataa ggttattgac tgttatagtt tgccaagaat
     7081 tgtgcgctgt acatatgacg atgataatac tgttacctta gtagttgctt ttttcaggga
     7141 aaaaagtttc aaggactgtc aagttgggga ttagaattta aggttatgca ccccctcttt
     7201 agagggggtt tttaagtaat gtatgatagc atttttagtg taatgcaaga caatgggagg
     7261 aatatcctgt gggacatgca aaagcagcaa ttgataagat aaatgtaaag aatctaccaa
     7321 agcatatcgc tattattatg gacggaaatg gtcgatgggc taagcaaaaa ggcctaccaa
     7381 gaattgcggg acattgggct ggagctgagg ccttgagaga tatagtacgc acttgctgca
     7441 agttaaacat tcaggttctt agtgtatttg cattttcatc agaaaattgg aaaaggcctg
     7501 ttcaagaagt aaacactatc atgaatttgt tgctttatta tttaaaaaaa gaggtcgatg
     7561 aacttgacaa aaacaatgtc aggattcatg tatcaggcgc ttgggaagaa ttgcccgaaa
     7621 gaatagtagg gcaaataaag aaaagcatac aacaaacgca aaataacaca ggtctcatat
     7681 taaatatagt attaaattac gggagccgca atgaaatatt atccgcttgc aaaaaactgt
     7741 ataaagacgt gcaaagtgat ataataagca ttgatggtat agatgaaagc acctttcaaa
     7801 attacttata tacccgggat ttgcctgatc cggatctgtt aatcaggcct agtggtgagc
     7861 taagaataag caattttttg ctgtggcaga ttgcatatac tgaattgtgg tttactgagg
     7921 tgtattggcc tgattttaaa tcagatgatt tgttaaaagc catactagat tttcagaaac
     7981 gcgagcgtag atatggtggt ttaaaatcat aaagcaggtg gatttatgat tacaagaatt
     8041 ataagtgctg ttgtcggtat aatactatta ggatacgtca taaatacagg tggttgggtt
     8101 ttttatgcag ctatttcctt cttgaatata tgtgcgctgt atgagctacg cagtgcattt
     8161 tcaaaaaaaa atatccacat aagatttttc ttaaattcag ctcttgcagt tgtactgttt
     8221 tattgtgcaa gttttaaaaa ctttgatttt atattgactt atctttttgc catattattg
     8281 ctcatgtttg agtttttata taatataatt gttagcaatg aaaatcatct taaagacatg
     8341 gtttttagcg ttttttcttt cctttacaca tcatttttgt tcatgtccat tattttggtt
     8401 cggaatatta gcttggggtt aaaacttaca tggtggatat tcattactac ttgggcatgt
     8461 gatacaggag ctttttttag tggaatgatc ataggaaaga cagctttagc tccggcagtt
     8521 agtcccaata aaactataga aggaagtttg ggcggcattt tgtcaagtgt agcggcaagt
     8581 gtgctttatt ccgtatattt tttacctgaa attaatactc tgcacgctat actttttggc
     8641 atttgtatag gtgttgttgc gcaaatcggc gatctatcag catcatttat aaaacgatac
     8701 tgccagataa aggatttttc aaatattatt ccaggtcacg gcggtattct cgataggctc
     8761 gacagcgcac ttttttcctt cccagtagca tatatattta taaagttatt gttacaaaaa
     8821 ggtggtctac tatgaaaatg agaaccactt attctcctat attttttcta gtccttgcag
     8881 ttgccgttac tgctatcgta attgtactgg ctattaagta ctttttgcca ttagtaattg
     8941 gaatatttat tgctattttg atagatccta ttgtaactta tcttgaacga aaattagaac
     9001 ttgacagggg aataataaca gttatagtcc tttcggctat attttgtcta acgggatata
     9061 tttcattatt gattattgct cgtttcacct ttgagcttgc acggtttaca aattttttac
     9121 cgactcagct gcgtcatttt aatgcactca tagataagtc atatttgtat ttatcaaact
     9181 tttttgctaa ggtgccagaa gacataatag tatatttaaa agcaaattta aatcaaatat
     9241 tgtctaccat atccgggtcc ctttcaaatt tatatacttt tctagtgaac aaaataggca
     9301 tggttccaaa tttgctagtg aatctactgg ttgtgataat ttttgttttc ttgttcagct
     9361 attttctaac gaaggacaag gataaaatta tacatataat aaagaacctg tttcctgaac
     9421 aattacacga aaaagtgaaa tcggtgcaat tagaactagt tttttctttc tttagattaa
     9481 tcaaagcaca gataattctg gttttaattt ctaccgttat tactgtatta ggcttttgca
     9541 ttttaagagt tgagtacgcg ctaactttgg gtataatatg cggtatatta gatataatgc
     9601 ctctgtttgg gcctagttta atatttatac catggattat atatttactt gttattggta
     9661 atgttaactt cgccataggc ttactgtttt tatatgtaga cgttatcgga agtagacaga
     9721 tgttccaagc aaaaatcatc ggtcgcaatc ttggtataga ccctctatta acattgcttt
     9781 ctatttattt aggagttaaa gttttcggat ggaaaggatt attcgtagga cctctggttg
     9841 tagtaatagt aagagctctc atacattgtg ggatcatacc tccattgaac aagccaacaa
     9901 aaaaagtgta agttgcggaa ggtgaagaaa attgcagact atactgtcat ctattttagt
     9961 ttttggaatg ctgatttttt ttcatgaact gggtcatttc atagttgcaa aactgtctga
    10021 cataaaagtc aatgaattta gccttggatt aggaccacag ttaatcagca tgaaattaaa
    10081 ggaaacgcac tattccatcc gattgcttcc attcggtggt tatgtaaaga tggaaggcga
    10141 agatactgat acgagcgatc cgagagcctt taacaataaa agtgcactgg tccgcttggg
    10201 cgttatactt gctggacctt taatgaattt cattcttgcc atattgctcc ttggaattat
    10261 cggtttttat tcgggagttc ctacaaggca agtgacggta actcccggag aacctgctga
    10321 gatatcaggg atacaagata atgacataat acatgcaatt gatggtcgta aagttggcca
    10381 ttgggaggaa atagtcgaga taataagcgg tagatataat gaaaaaatca gcgtcacagt
    10441 cattagagat ggaaaataca ttgattatga tgttacgaca accgttgagc caaaaacgca
    10501 gcggggcatt atcggtatta aatcagtaat gcaaaagcac tctgtattag agagcattaa
    10561 gtacggttat aaaaagactg ttttgatatt gaagctaata ttagtgggta tatatcaggt
    10621 gctgacaggc aaagtgaaag ccgatgtagt agggccggtt ggtctggccc atatagtagg
    10681 agaagctgct aagatcggtg tttatcaggt gctgtatatt gcagctgtta taagcgcaaa
    10741 tttgggtctc ttcaacctgt ttcctattcc tgctcttgat ggtggcagag gaatattttt
    10801 ggtatttgaa ctgttaagag gcaagcctgt tgaaccggaa aaagaagggt taatccattt
    10861 tattggattc gcacttttga tgttcctgat gatactgata ttatttaagg atttaaggga
    10921 attgtggttc tgagaaagtt agctaatcac caggctttaa ttgatagtag caagtacata
    10981 tgtataggta agagggagaa ctatgctgga atttagcaca attaaaaggc ggaaatctag
    11041 aaaggtaatg atcaaggatg taggtgttgg aggagatagt cccattacag tccaatctat
    11101 gactaataca aagacggatg atattgaaag cactgtaaat caaatcatca gtttagaaaa
    11161 tgctggatgt gacatcatac gagtggcggt tcccgatatg gcaagtgccg aagccatttc
    11221 aaagataaaa gagcaaatcc atataccgat tgtggcagat gtgcactttg attatcgatt
    11281 agctatcgag gctataaagc agggagcaga tgggcttagg attaatcctg ggaatatcgg
    11341 cgatgccgaa ataattaaaa aagttgttga aacggcaaaa tcatttgatg tgccgattag
    11401 gatcggcgtc aattcaggtt ctttggaaaa ggatctactt gaaaaatacg gcggcccctc
    11461 acctgaagct ttggtggaaa gtgcattgag gcatgtggaa attttagaga atatgaattt
    11521 tgagcatatt gtaatttcat taaagtcttc agatgtgctc tcatgtatac attcctacaa
    11581 attgattgct caaaaagtgg actatccttt gcacctgggc atcacggaag cgggaactct
    11641 cctaaccgga acaattaagt cttcagtagg cttagggatt ttactgtatg aaggaatagg
    11701 tgatactatc agagtatctt tgacaggtga acctgtagac gaggttaaag tgggttttga
    11761 aatactaaag agtttaaatt tgagacaata tggtattaat tttatatcat gcccaacttg
    11821 tgggagaaca cagatcaatt tgattaaaat agccaatgag gtacaggaaa aactgtcaca
    11881 cataaaaaaa ccgttaaaag ttgccataat gggctgcatt gttaatggtc ccggagaagc
    11941 tagagaggca gatattggta ttgccggtgg aaaaaagcgg gttgcaatat ttaaaaaagg
    12001 tgagataata aaaactgtgt cggaagaaga tgctgtttcg gaactggtga aagaaataaa
    12061 acacatgata aatgaataga aagttgtttt ggaaaggagc attcttatgg aaaatgactt
    12121 tgtaaaagaa attacgccca aatctgagga tttttcccaa tggtatgttg atgtcatact
    12181 taaggctgaa ctggcggatt attcaccggt tagaggatgt atggtaataa ggccttacgg
    12241 ctacagtatt tgggaaaaaa tgcagcagct tttggacagc agattcaaag caacaggtca
    12301 taaaaatgcc tatttccccc tcttcattcc cgaatcactt cttcaaaaag aggcagaaca
    12361 tgtggaaggc tttgcgccag aagttgcatg ggtcacccat ggaggtaatg aggaattggt
    12421 agagcgcctt gcggttagac caacatcaga aactatcatt tgccacatgt attccaaatg
    12481 gataaagtca tggcgagact tgccggtcct tataaatcaa tggtgcaatg tagttcgctg
    12541 ggaaaaaagt acaaaaccct ttttacgtac tgctgaattc ttatggcagg agggccatac
    12601 agctcatagc agttttgaag aagcggaaga agaaacatta aggatgttgg aagtatatcg
    12661 tgattttgtt gagacagatt tgtcaatgcc cgttataacg ggagtaaaat ctgaaaatga
    12721 aaagtttgcc ggagccttaa aaacatatac cattgaagca ctgatgggtg atggtagagc
    12781 acttcaggct ggcacttccc acaatctggg tcagcacttt gcaaaagcct atgatattac
    12841 ttatcttgat aaggatggaa aactaaagca tgtttggcaa acatcttggg gagttacaac
    12901 tcgtctaata ggagcaatta ttatggtcca tggtgatgac aggggcctca agcttcctcc
    12961 aaagattgcc cctattcagg cagttattgt gcctattttg ggaaaggagt ctgaaaccgt
    13021 acttaataaa gctagggagt tgtactccat tctcaataag gagattaaga ctgaattaga
    13081 tgatagaaat gaatacactc cgggatggaa atttaacgag tgggaaatga aaggtgtgcc
    13141 tgtaagaatt gagatagggc caagggatat aaagaacgag caggttgttt ttgtgcgaag
    13201 ggacactgga gaaaagatca cagtcagtga atcggaactg cttccacgct taaagagttt
    13261 gctagatgaa atacaagcaa atatgtttag acaggcacaa gtttttctta acgaaaatat
    13321 tagagaagtg gacaactttg atgagtttaa gcaaatcatt gatgaaaaaa gaggttttat
    13381 aaaagcacat tggtgcggga atactgaatg tgaaaagaag gtaaaagaag aaacaggcgc
    13441 caccttgaga tgcattccat tttgtaaaga aactatcagc ggaaagtgta tagtatgtga
    13501 taaacaatct gatacagttg catattttgc aaaatcatat tgattccata gcatagggcg
    13561 ccctaattat acggggtgcc tcaaaacttt cttgttctag agcagagtcc atgtattgtt
    13621 gaaattggtc caaagctgtg ttaaagtaaa agtaagacta caatgatgag ggtgataata
    13681 tgcccaaaat agccgttgta atacctgctt acgatgaaga aaagactatt ggcaacgtct
    13741 taaaaccggt atcagctata agcatgatag atgatattat agtagttagt gacggttctc
    13801 acgatagaac cgcaaacatt gctcgatcat ataaggcgcg agtaatagaa tttgataaga
    13861 atcacggaaa aacatatgct gttttgtcag
//
LOCUS       DAONUO010000008        13748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_3404_length_13748_cov_3.744760, whole genome shotgun sequence.
ACCESSION   DAONUO010000008 DAONUO010000000
VERSION     DAONUO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3404_length_13748_cov_3.744760"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK768_00660"
     CDS             <1..246
                     /locus_tag="PK768_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59514.1"
                     /translation="LRKAFKQHMTLNKSDLVAMLIAALDETLFELDLEAEKDEFKEAE
                     EEIKRGQVQSATAHLIIYFLYYLCVYSLLNSGSFAPK"
     gene            complement(285..371)
                     /locus_tag="PK768_00665"
     tRNA            complement(285..371)
                     /locus_tag="PK768_00665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(335..337),aa:Leu,seq:caa)
     gene            579..2210
                     /gene="hcp"
                     /locus_tag="PK768_00670"
                     /gene_synonym="priS"
     CDS             579..2210
                     /gene="hcp"
                     /locus_tag="PK768_00670"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HQA59515.1"
                     /translation="MRMFCYQCQEASRGVGCTVKGVCGKTAEVANLQDLLIYTLKGIS
                     DIVQKGNLNVQDLNDLNHEMVKALFMTITNANFDDEAIENEIKKMITYRDELRAKANV
                     DNLHDAARFQVDSKVDMLEKASSVGVLSTKDEDIRSLRELITYGLKGMAAYTHHALNI
                     GKENNEIYAFVYEALAATLDDTLTADELVALTLKTGEYGVKAMALLDEANTSKYGNPE
                     ITEVNIGVRDNPAILISGHDLTDLEQLLEQTQGTGVDVYTHSEMLPAHYYPAFKKYDN
                     FVGNYGNAWWKQVEEFESFNGPILFTTNCIVPPRKEEIRARIFTTGAAGYPGCLHIEA
                     DEDGKKDFSPIIEMAKRLNPPKEIENGKIIGGFAHAQVFALADKVVDAVKSGAIKKFF
                     VMAGCDGRMKSREYYTEFAKKLPKDTVILTAGCAKYRYNKLDLGDINGIPRVLDAGQC
                     NDSYSLALIALKLKEIFELNDINELPIAYNIAWYEQKAVIVLLALLYLGVKNIHLGPT
                     LPAFLSENVAKVLVENFGIAGIGTVDEDLALFMGQ"
     gene            complement(2278..4023)
                     /locus_tag="PK768_00675"
     CDS             complement(2278..4023)
                     /locus_tag="PK768_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQA59516.1"
                     /translation="MKTDFLMDIKVKDALRKEVILVHSNMTVENLNEIMLLKKQEEVL
                     VVDSDDKIVGIITRNDLAKSLARGIDLETQIKDIMTPDVFCVHPDKPLLQARGEMRNL
                     AIGRAPVLDERGQLLGILTGKSICDTFSGQLEKVVHFQSLIMDSIKTAICILNDKCEI
                     LAYNKAFEQLFKPYKIFKLAPHHFLPAKLAEKIKKGETPLDEIYFENKDGRKFLLKTC
                     PFKFDEKLSGMIMNIEEISDVINIITELEKANQRMLFLEKQLSTTKEQTAFGKLSSKN
                     PKMIEVIELAKRTAMTDVPILIKGEAGTGKAMLAATIHEFSERRKGPFIKVNCAAIPA
                     NIFESELFGLDIGTFSETAYSGKIGLIELANKGTIFFENIHEMPFNIQMKLESFIKNK
                     TFYKTDGNTPIKTDVRIIASTDKELGSLVGKDEFYEKLYEDINIVSIEIPPLRERSED
                     IVDIVKFFIEEFEQQYNKKVKHIDSEVLKTFMDYSWPGNIRQLKNVVERLVILCEDGS
                     ITNDDLPREVKNEEDYHTVLQDVSGLEEATDIAERQVIIDALKKCAYNKTKTAEYLKI
                     SRSTLYNKMREYNIQ"
     gene            4211..5134
                     /locus_tag="PK768_00680"
     CDS             4211..5134
                     /locus_tag="PK768_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HQA59517.1"
                     /translation="MKIKSIISFIMAIFVFSTCAKAAPPCSCGEDINLKLEDPPIMGQ
                     DVREIQIQLYRLGYFKDSINGIYDKTTADAVKEFQRREGLPADGVFGPKTFRKLAELY
                     EQPVAQIETEKPQGEVSLVILTLERELVVLDDGKVFKRFPVAVGKFNTPTPIGLFTIT
                     QKDAWGEGFGSRWMRLSVPWGVYGIHGTNKPWSIGSFESGGCIRMHNVHVEQVYEWVK
                     IGTKVFIVGGVDGPFTFGLYPLTQGSKGSDVMEIQKRLSGHGFYNGPIDGIYGYNTRE
                     AVIAFQKAHGLNPSGNVDTFTYEKLGIILFE"
     gene            complement(5118..5777)
                     /locus_tag="PK768_00685"
     CDS             complement(5118..5777)
                     /locus_tag="PK768_00685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQA59518.1"
                     /translation="MKYKAVIFDLDGTLLDTLQDLADSMNHVLESEGLPIHETEKYRY
                     FVGNGAYNLVKRSLPPDKQEESYIRYCQTLFKKEYGKRWADTTKPYEGIPELLDKLTD
                     LGFKLAVLSNKPHEATLLVVNKLLPNWTFDAVFGEREGVPRKPDPAGALEISQLFGIP
                     TNEFIYFGDTSTDMVTAKAAEMFAVGVLWGFRTADELMDNGADLIIEKPLDIFKFIQT
                     E"
     gene            6070..6948
                     /locus_tag="PK768_00690"
     CDS             6070..6948
                     /locus_tag="PK768_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQA59519.1"
                     /translation="MNMEKAKNRIMEINKVLWTILALNLIVSIMKLVVGYAISSASMV
                     ADGFHSFSDCSSNIIGLIGTALAAKPADECHPYGHKKFETFTSIIIALLLFIVSINII
                     KSSVSKLINPVIPHVDIYSFIVMIITIAINIFVIIYETKQGKKLKSDILISDAIHTKT
                     DVYVSLSVILSLIAVKLGYPILDPIISFVISIVIIKAGVEILINSSKVLCDAAVLDPQ
                     DVIDLVSKIDGVLDCHRVRSRGREDDFSLNLHIVVDPSMPVSQSHRLHHEIEEEIRKH
                     FSGVHYIDIHIEPLTD"
     gene            complement(7188..7274)
                     /locus_tag="PK768_00695"
     tRNA            complement(7188..7274)
                     /locus_tag="PK768_00695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7238..7240),aa:Leu,seq:cag)
     gene            complement(7386..8183)
                     /locus_tag="PK768_00700"
     CDS             complement(7386..8183)
                     /locus_tag="PK768_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQA59520.1"
                     /translation="MLKELSKRQQRLIKNLKESKTARAKNRCFFVEGVRSVEEVFDSD
                     YDIRFVVLSDSFLRSDDVLLQKIKRKVSDDKLYRVSNRTFSELTDTVTPQGILAVINF
                     ADEKIDDILKDNFLIVALDRIQDPGNMGTIIRTADAAGTDGIIVGKGCVDIYSPKVVR
                     SAMGSLFHIPIVQTEDLRETLIELKQKGGQVVTTHLSATQDYSDVNYKTSTVIVMGSE
                     AEGVSQEIYDISDELIKIPMPGQAESLNVAVAHGIIAFEAVRQRRQA"
     gene            complement(8198..8851)
                     /locus_tag="PK768_00705"
     CDS             complement(8198..8851)
                     /locus_tag="PK768_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HQA59521.1"
                     /translation="MRQFLVVGLGRFGTSLAKTLYNLGYDVMGIDSNEEIIQNIADSI
                     THAVQADATDENTLKALGVRNFDVAIVSIGNDIQSSILITIMLKEMGIKYVVAKAQSQ
                     LHGKVLYKIGADRVVFPERDMGVRVAHNLVSSNILDYIELSPEYSIVEIAAIPAWHNK
                     SLGQLDMRRKYGLNVMAIKRGNNDVKISPHADDLISENDVLVVVGRKSDIEKLERHI"
     gene            complement(8867..10273)
                     /locus_tag="PK768_00710"
     CDS             complement(8867..10273)
                     /locus_tag="PK768_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HQA59522.1"
                     /translation="MPRQTLNKKRPRSLFLDTTGAISINILHEGLKRLKPTQILAVGF
                     ASLIIVGAFLLSLPAAAKSGESIGLLNALFTATSAVCVTGLTVIDTNAFSLFGQIVIL
                     ILIQIGGLGIMTMATFIFLLLGKKITLRDRLVMQEALNQLTLSGLVKLTRQILLLAFV
                     LEGLGALLFIYRFTKYFDFGQSLYYGLFHGVSSFTNSGFDVVGFGSLAPFIEDPIINI
                     VTMFLNECGGLGFTVIYDIVSTRNFKRLSLHSKVVLTMTLILMLLGCIMFYTLEWNNP
                     STLGRLTPSGKMLAAAFHSVSTRTSGFNTFSLEDLTTPAKYLSIFLMFIGSSPASTGG
                     GIKTSTFALIIVTIYTVMVSKEDVEIFNRRISTDNIFKAVVIAIVSALLIFVSSFILT
                     ITEQADFLPIFFEATGAYSTVGLSLGITQRLTDYGKYIIILTMFTGRLGPLTLAMALG
                     SQRKKALRKFPEGRILIG"
     gene            complement(10251..10607)
                     /gene="rplT"
                     /locus_tag="PK768_00715"
     CDS             complement(10251..10607)
                     /gene="rplT"
                     /locus_tag="PK768_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQA59523.1"
                     /translation="MPRVKRGVTARKRHKKIIKLAKGYRGSQSKLYRPANQAVLKALA
                     HSYRGRKLKKRDFRKLWIARINAAARANGISYSRFINGLKKAGVQINRKMLSEMAIHD
                     ASGFSQLVDIAKANTQ"
     gene            complement(10625..10822)
                     /gene="rpmI"
                     /locus_tag="PK768_00720"
     CDS             complement(10625..10822)
                     /gene="rpmI"
                     /locus_tag="PK768_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQA59524.1"
                     /translation="MPKVKTHRGAAKRFKVSKTGKIKRAKAFKSHILTKKTAKRKRHL
                     RKPAVLSKADQKRIKQVIPYM"
     gene            complement(10828..11340)
                     /gene="infC"
                     /locus_tag="PK768_00725"
     CDS             complement(10828..11340)
                     /gene="infC"
                     /locus_tag="PK768_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQA59525.1"
                     /translation="MQVNHEIKAREVRLIGPDGKQLGIMPLKEALRHAQEAQLDLVKV
                     ASNAKPPVCKIMDFGKYKYEQSKREKEARKNQKVINIKEIRMNPTIDEHDFQVRLKNT
                     LKFLKEGNKVKVSIRFRGRQMTHTQLGEEVLNKMIELTQELGVVEKPPKMEGRNMVMV
                     LSPKQTKTKE"
     misc_feature    complement(11380..11490)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(11538..13448)
                     /gene="thrS"
                     /locus_tag="PK768_00730"
     CDS             complement(11538..13448)
                     /gene="thrS"
                     /locus_tag="PK768_00730"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQA59526.1"
                     /translation="MSEIQVTLKGGVKKTYPKGISLLEIAEDISKKLAKEALVAKVDG
                     VLRDLSYKVQDDSVVEILTFDDEEGRKVFWHSTSHVMAQAVKRLFPDAKLAIGPAIDE
                     GFYYDFDMEESFSPSDIEQIEKEMAKIIAENYSFSRKDVSRDEALSDLEEQNEPYKVE
                     LVQALEDDANISFYNQGEFSDLCAGPHIPSTGKIKAFKLTSLAGAYWRGDEDNKMLQR
                     IYGISFPKKSMLDEYLHRIEEAKKRDHRKLGRELDLFSLHEEGPGFPFFHPKGMVLWN
                     ILIDFWRKEHQKRGYQEIKTPIILNEELWQRSGHWEHYKDNMYFTKIDDMAYAVKPMN
                     CPGGILMYKSNSHSYRDLPIRLGELGIVHRHEKSGVLHGLMRVRCFTQDDAHIFMMPS
                     QIEEEIIGVIDLVDYFYKIFGFEYHVELSTRPENSMGSDELWEQATNALQKALEKKGM
                     PYKINEGDGAFYGPKIDFHLKDSLGRTWQCGTIQLDFQMPERFDLSYIGPDGEKHRPV
                     MIHRVIYGSIERFIGILIEHYGGAFPMWLAPVQARILPISEKHLEYARKVKQQLEEAG
                     IRVELDERNEKIGYKIREAQMQKTPYMLIVGDKEAEENTVSVRVREKGDTGQQSIESF
                     IEEVKELTAKFN"
     misc_binding    complement(13499..13674)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3861 a         2959 c         2542 g         4386 t
ORIGIN      
        1 ctgcgtaaag cattcaagca gcatatgacc ctaaataaaa gtgatcttgt ggcgatgctc
       61 attgccgctc ttgatgaaac gctatttgag cttgatttgg aagcagaaaa agatgagttt
      121 aaagaagcag aggaagaaat aaaaagagga caggtacagt ctgccactgc gcatcttatt
      181 atatattttt tatattacct ctgcgtgtat tctcttctca acagcggctc tttcgcccca
      241 aaataaaata gaccgactct cgtcggcctt aaatttctta agtttggtgc cgaaggcggg
      301 agtcgaaccc gcacgggcgt gagcccacag gattttgagt ccagcgcgtc tgccaattcc
      361 accacttcgg cacgtattta tgtcaaatgc ataataatat tatcatatca cccatgaaaa
      421 ttcaagagta tgtttttatc tctttgtgat ttctgtcaca gactagcatc ggctttttca
      481 cgtattatat ttttaacaga caaatgatta gtgcttgccg gtataatgtg ttttgacata
      541 ttatattggt aacggtaact attaaggagg tagtctttat gagaatgttt tgttatcaat
      601 gccaggaagc atcgagaggc gtagggtgta ctgttaaagg ggtatgcggc aaaacagctg
      661 aagttgctaa tctgcaggac ttattaatct acacactcaa aggtatttct gacattgttc
      721 aaaaaggcaa cttaaatgtc caagatttaa atgatttaaa tcatgagatg gtgaaggcac
      781 tttttatgac tattaccaat gctaactttg acgatgaagc cattgaaaat gaaattaaaa
      841 agatgattac ttatagagac gaattgaggg caaaagcaaa tgtagataat cttcatgatg
      901 ctgcccgctt tcaggttgat tcgaaagtgg acatgcttga aaaggcgtct tccgttggcg
      961 tactttctac aaaagatgaa gatatccgct ctttgagaga attaattaca tatggtctta
     1021 agggaatggc agcatatact caccatgcct taaatatcgg aaaggaaaac aatgaaattt
     1081 atgcatttgt ttatgaagca ttggctgcaa cccttgacga cacactcact gcagatgagc
     1141 tagtagcttt gactttaaaa accggtgagt acggtgtgaa agcgatggcc ctcttggatg
     1201 aggccaatac ttcaaagtac ggcaatcctg aaataacaga agttaatatc ggtgtcagag
     1261 acaatcctgc catcttaata tccggccatg acctgactga cctggaacag ttattggagc
     1321 agacgcaggg caccggagta gatgtctata ctcatagcga aatgttgcct gcccattatt
     1381 atcctgcctt taagaagtat gacaattttg taggtaacta cggcaatgcc tggtggaaac
     1441 aagttgaaga attcgaaagc tttaacgggc caatcctctt tacaaccaac tgtatagttc
     1501 cgccgagaaa ggaagaaatt agggcaagga ttttcaccac cggtgcagca ggttatcctg
     1561 gctgcctgca tatagaagcc gatgaggacg gcaaaaaaga tttttcacct atcatagaaa
     1621 tggcaaagag actcaatcct cccaaagaaa ttgaaaatgg taaaattatt ggcggttttg
     1681 cccatgccca agtctttgcc ctggcagata aagttgtcga tgcagtaaaa tccggtgcaa
     1741 ttaagaaatt cttcgtaatg gcaggatgcg acgggcgcat gaaatcaagg gaatactaca
     1801 ctgaattcgc aaaaaagctt cccaaggaca cggtaatact tactgccggt tgcgctaaat
     1861 accgctacaa caagctggat ttaggtgaca ttaacggcat acccagggtt ttagacgcag
     1921 gacagtgtaa cgactcatat tctctagctt tgatcgcact gaagttaaag gaaatatttg
     1981 aattaaatga catcaacgaa ctgccgatag catataatat cgcctggtac gaacaaaaag
     2041 ccgttatagt actccttgcc ctattatatt taggcgtgaa aaatatccat ctcggcccta
     2101 cactgccagc tttcctttcg gaaaacgtag caaaagttct tgttgaaaac tttggtatcg
     2161 ccggaattgg aacggtagat gaagatttgg cattgttcat ggggcagtaa actgacaagc
     2221 tcctagcttc ctttaaggaa ggctaggagc ttttttatgt caaggtcaac atcaatttta
     2281 ctggatatta tactctctca ttttgttgta caatgtactc ctcgaaatct tcaaatactc
     2341 tgcggtcttt gtcttattgt aagcacattt tttcaaagcg tctattatca cttgcctttc
     2401 tgcaatatca gttgcttcct ctaaaccgct aacatcctgc aatacggtgt gatagtcttc
     2461 ctcatttttc acttcccttg gcaggtcatc gttggtaatt gacccatctt cgcacaggat
     2521 taccaatctc tccactacat ttttcaattg ccttatattg cctggccagc tgtagtccat
     2581 aaaagttttt aatacttcag aatcaatgtg tttaaccttt ttattgtatt gctgctcaaa
     2641 ttcctctatg aaaaatttta caatatctac aatatcctca ctgcgctctc tgaggggtgg
     2701 aatctcaatt gagactatat ttatatcttc atacagtttt tcgtaaaact catctttccc
     2761 aactaagctt ccgagctctt tatccgtgga cgcaattatt cttacatcgg ttttaattgg
     2821 ggtattgcca tcggtcttgt aaaatgtttt attttttatg aaactctcaa gcttcatctg
     2881 tatattaaaa ggcatctcat gtatattttc aaaaaagatg gtacctttat tcgccaactc
     2941 tatcaagcca attttgccgg aatatgccgt ttcactaaat gttcctatat ccaggccaaa
     3001 aagctcactt tcgaaaatgt ttgccggaat tgccgcacaa ttcaccttaa taaacggtcc
     3061 ctttcgcctt tcgctaaatt cgtgtatggt agcagcaagc attgctttgc cggtacctgc
     3121 ttcacccttt atcaaaatag gaacgtcggt catggccgtt cgcttggcaa gttcaattac
     3181 ttcaatcatt ttggggtttt tgctgcttag cttcccaaaa gccgtctgtt ccttagtggt
     3241 actcaattgt ttttccaaaa acaacatcct ctgattagcc ttctccaatt cggtgataat
     3301 atttataaca tcagatattt cttctatatt cataatcatt cctgacaact tttcatcgaa
     3361 cttaaacggg catgtcttca ggaggaattt ccggccatct ttattctcaa aatagatttc
     3421 atcaagaggt gtttcaccct tttttatttt ctctgccagt ttagccggta aaaagtgatg
     3481 tggtgcaagt ttaaatatct tatatggttt aaacaattgt tcaaaggctt tgttgtaagc
     3541 taatatttcg catttatcgt ttaagataca tatagctgtt tttatgctat ccataattag
     3601 gctttgaaaa tgcactactt tttctaactg ccctgaaaat gtatcgcata tgctcttgcc
     3661 cgtcagtata cctaggagct gtcccctctc atctaaaacc ggggctctgc caattgctag
     3721 gttgcgcatt tcgcctcgag cctgaagcaa aggtttatca ggatgcacac aaaacacatc
     3781 aggagtcatg atgtctttaa tctgggtttc taagtcaatg cccctagcaa gacttttggc
     3841 aagatcattt cttgtaatta tgcctactat cttatcatca ctgtcaacga ccagcacttc
     3901 ttcctgtttt tttaaaagca ttatttcatt taaattctca acagtcatat tgctgtgaac
     3961 caaaataact tcttttctta gagcatcttt aacttttata tccattaaaa aatccgtttt
     4021 caaagccgtt tcctcccaga tatttcctat gtgtaaaatc ttatacagta tattaatatt
     4081 tctacattag cggtcaaaaa cctctttgat tttaaaaaaa attatatcca gatatgtaaa
     4141 aagtcaccta tatggtatgg ctgaattaaa acggtaaatt caacaaaaaa tatccctatg
     4201 gggtgataaa atgaaaatta aaagcattat atcatttatc atggccatat ttgttttctc
     4261 aacttgcgct aaagctgctc ctccctgctc gtgtggagaa gacataaatt taaaattgga
     4321 agaccctcct atcatgggtc aagatgtgag ggaaatacag attcagcttt acaggctggg
     4381 ttattttaaa gattcaataa atggaattta cgacaagact acggcggatg ctgtcaagga
     4441 atttcaaagg cgggaaggtc taccagcaga cggagtcttt gggcctaaaa cttttagaaa
     4501 actggcagaa ctttatgaac aaccggtggc acagatagaa acagaaaaac cacaaggaga
     4561 agttagttta gtcatcttga ctttagaaag ggaacttgtg gttttagacg atggcaaagt
     4621 ttttaagcgc ttccctgtgg cagttggtaa gttcaacaca cctactccca taggactgtt
     4681 tacaattaca caaaaagatg cttggggaga aggtttcggt tcgcgctgga tgcgattgag
     4741 tgtcccctgg ggcgtttacg gaatccacgg caccaataaa ccttggagca tagggagctt
     4801 cgagtccggt ggatgtatac gaatgcacaa tgtccatgtg gaacaagtgt atgaatgggt
     4861 aaaaatcggc acaaaagtat ttattgtggg aggagtagac gggcccttta cctttggctt
     4921 atatccgctc actcaaggct ccaagggttc agatgtgatg gagatccaaa aacgcctatc
     4981 gggacatggc ttttataacg gccctattga tggaatctat ggatacaata ccagggaagc
     5041 ggtgatagcc tttcaaaagg ctcatgggtt aaatccttca ggcaatgtag atacattcac
     5101 gtatgaaaaa ctagggatta ttctgtttga ataaatttaa atatatcgag gggcttttct
     5161 attattagat cagcaccatt atccataagc tcatcagcag tcctaaatcc ccacagcacc
     5221 cctactgcaa acatttccgc agcttttgcc gttaccatat cggtactcgt atcaccaaaa
     5281 tatatgaact cattggtggg aatgccgaaa agctgggata tctccaaagc ccctgcagga
     5341 tccggctttc ttgggacacc ctctcgctct ccaaaaaccg catcgaaggt ccaattgggc
     5401 aatagtttgt ttaccaccaa taatgttgct tcatgaggtt tatttgagag cacagctagc
     5461 ttgaatccca agtcagtgag cttatccaat agttcaggga taccttcgta aggtttagta
     5521 gtatcagccc acctcttccc gtattctttc ttaaataatg tttggcagta tcttatatac
     5581 gattcttctt gtttatcagg tggaaggctt ctctttacga ggttatacgc tccattgcct
     5641 acaaaatatc tgtacttctc cgtttcatga atgggtaaac cttcactttc aagcacatgg
     5701 ttcatagaat cagctaagtc ttgtagggta tctaataaag tgccatctaa gtcaaaaatt
     5761 actgctttat acttcaaaaa aatggctcct ttctataatt atgtaattgt agatatgtcc
     5821 ttaatttaaa acataatcta ttttatgctt tttttcaagc aagagaagca caggaggttt
     5881 tatcaaggga cagtattgac tgaaacaagc atagtatgtt atcattttat tgataacata
     5941 ttttttacat ttaaatcgga acatagcaac aagtttgggc atgtgggaca ttaaacccac
     6001 aattttttta tattatagtt gttgtcaagt aagcattttt atctcataaa catcgtgggg
     6061 gcacttgtga tgaatatgga aaaggctaaa aacagaatta tggaaattaa caaggtcctc
     6121 tggaccattc ttgcgcttaa cttgatcgtc tcaataatga aactagtggt gggatacgca
     6181 atatcttcag ccagtatggt tgcagacggt tttcattcct tttccgattg cagttcaaat
     6241 atcataggtt taataggaac tgctttggct gcaaagccgg cggacgaatg ccacccttac
     6301 ggccacaaaa aatttgaaac atttacttcg atcataatag cactgttgct gtttattgtc
     6361 agcataaata ttattaaaag cagtgtatcg aagctaataa atcctgtcat ccctcatgtc
     6421 gatatataca gctttatagt aatgattata acaattgcaa ttaatatttt tgtaattatt
     6481 tatgaaacaa agcagggcaa aaaacttaaa agtgatatct tgatttcaga tgctatccac
     6541 acaaaaactg atgtgtacgt ttccctgtcc gttatcttaa gtctaattgc agttaaattg
     6601 ggctatccca tcctggaccc gattatttca tttgtaattt caattgtaat tataaaagct
     6661 ggagtggaga tactgataaa ctccagtaag gtcttatgtg atgctgcggt cctagacccg
     6721 caggatgtga tagatttggt atcaaaaatt gacggtgtct tggactgtca ccgggtgcgc
     6781 tctcggggaa gggaagacga cttttctcta aacttgcaca ttgtagtaga tccttccatg
     6841 cctgtaagtc agtcccacag actacatcat gaaattgagg aagaaattcg aaagcatttt
     6901 agcggtgtcc attatattga tatacatata gaaccattga ctgactagca ctatcaatct
     6961 ccagaccata atattgtttt tttactctgc ctcaaccata tccctctact tttttagctg
     7021 tttccgcata tccggaatca tgtacccacc ttacgatgtt ttgcccggta gtgtgatgaa
     7081 atcttattat atcttatatt tcaatgtgat aaaggatcta aaacaactaa aaacagggtt
     7141 taaaaactag ttcaactagt tctaaaccct gttgtctttg tcataattgg tgccgaagac
     7201 aggattcgaa cctgcacgcc cttgcgggca ctagaccctg aatctagcgc gtctgccaat
     7261 tccaccactt cggcacttat atttgtcgga tgcataatta atattaccac atcagttttt
     7321 aaaattcaag agcttttgtt taactgctat tatcgctttc gtacacaatt gttgtgctga
     7381 cggctttatg cttgccttct ttgccttacc gcttcaaagg ctatgatgcc gtgggccaca
     7441 gctacattta acgactctgc ctgaccgggc atgggaattt taatcagctc atcggaaatg
     7501 tcatagattt cttgtgatac accttcagcc tccgagccca ttactatgac ggtacttgtt
     7561 ttatagttta catcagaata atcttgtgtc gccgataaat gtgtagttac cacttgccca
     7621 cctttttgtt ttagctcaat aagcgtttcc ctcaaatcct cggtttgcac tatgggaata
     7681 tgaaataaag aacccatagc tgatcgcaca acttttgggc tatatatatc gacacagcct
     7741 tttcctacaa taattccgtc tgttcctgca gcatcagcag tccgtattat tgttcccatg
     7801 ttgcccgggt cctgtattct gtccaaagct acaatgagaa agttatcttt taatatgtca
     7861 tcaatttttt catcggcaaa attgatgact gccaaaatcc cttggggtgt tacagtatct
     7921 gtaagttcag agaaagttct atttgaaact ctgtataatt tgtcatcaga aacttttctt
     7981 tttatttttt gcagcaaaac atcatcagat cttaaaaagc tatctgataa tacaacaaat
     8041 cttatgtcat aatcagaatc aaaaacctct tcaacacttc ttacaccttc aacaaaaaaa
     8101 cagcggtttt ttgctctagc ggtcttagac tctttcagat ttttaattag cctttgttgc
     8161 cttttagata gttctttcaa catttatctg cacctggtca aatatgtctt tccaattttt
     8221 caatgtcaga tttacggcct accaccacaa gcacatcatt ttctgatatc aagtcatctg
     8281 catgaggcga tatttttaca tcattattac cacgttttat agccatgaca tttaagccgt
     8341 atttccttct catatccagc tgaccaaggc ttttgttatg ccatgccggt atggcagcga
     8401 tttctacgat ggaatactcc ggtgagagct caatatagtc taatatatta gatgaaacta
     8461 aattgtgagc tacccggaca cccatatccc tttccggaaa gaccactctg tcagctccga
     8521 ttttatacag gaccttccca tgcaattgac tttgagcttt tgctaccacg tactttatac
     8581 ccatctcttt taacataatt gtaatcaaaa tactcgattg aatatcattg cctatgctaa
     8641 ctattgccac atcaaagttt ctaacaccca aggcctttag ggtattttca tccgttgcat
     8701 ctgcctgtac tgcatgtgtt atgctgtctg ctatgttctg tattatttct tcattgctgt
     8761 caatacccat tacatcataa ccaagattgt acaatgtttt ggccaagctt gtcccaaaac
     8821 ggcccaaacc cacaactaaa aactgcctca tgcaccaaac ctccatttaa ccaattagga
     8881 tccgcccctc tggaaacttt ctcaaagctt tttttctttg actccctaaa gccatggcaa
     8941 gagtaagtgg tcccaatcgg cccgtaaaca ttgtcagtat tattatgtat ttgccataat
     9001 ccgtaagtct ttgggtaatg cctaagctta aacctactgt tgaataagct cctgtcgcct
     9061 caaaaaaaat cggtagaaaa tctgcctgtt ccgtaattgt cagtataaat gagctgacaa
     9121 aaatcaacag tgcagacaca attgctataa caaccgcttt aaatatatta tcagttgata
     9181 tgcgcctatt aaatatctcg acatcttctt tggataccat tacagtatat atcgttacaa
     9241 taataagagc aaaggttgaa gtctttatac cgcctcccgt tgaagcaggc gacgatccta
     9301 taaacattaa aaaaatggat aaatatttgg ccggcgttgt taaatcttca agactaaatg
     9361 tgttaaagcc ggaagtcctt gtggataccg agtgaaatgc tgccgccagc atctttccac
     9421 ttggtgtaag cctacctaat gtacttgggt tgttccattc caatgtataa aacattatac
     9481 aacctaacag catcagtatt aatgtcatag taaggacaac cttggaatgc agggatagac
     9541 gcttaaaatt tcgtgtggaa acgatgtcgt aaattactgt aaatccaaga cctccgcatt
     9601 cattaaggaa catcgttaca atatttatta tcgggtcttc aatgaatggt gccagacttc
     9661 caaagcccac tacatcaaag ccagaattag taaatgaaga aactccatga aacaggccgt
     9721 aatataggct ctggccaaag tcaaaatatt ttgtaaagcg atagataaac agcaacgcac
     9781 caaggccctc taatacaaag gctagaagta gaatttgcct tgtcagctta accaagcccg
     9841 ataatgtaag ttgatttagg gcttcctgca ttacaagtct atctctcaaa gttatctttt
     9901 tccctaataa aaggaaaata aaagtagcca tggtcattat cccaaggccg cctatttgaa
     9961 ttaagatcag aataactatc tgaccaaaga gggaaaaagc attagtgtca attacagtaa
    10021 ggcctgtaac acatacagcc gacgttgctg tgaaaagggc atttaacaag cctatacttt
    10081 ctccactttt cgcagctgcc ggcaggctta aaaggaaagc tcctacaatt atcaaagagg
    10141 caaaaccaac ggctaatatt tgagtaggct tgagtctttt taagccttca tgcaatatat
    10201 ttatgctaat cgcccccgtg gtatctaaaa aaagcgacct aggccgcttt ttattgagtg
    10261 tttgccttgg caatatctac cagctgcgaa aagccgctag cgtcatgtat tgccatttcg
    10321 gacagcattt tgcgattaat ttgtacaccg gcttttttaa gcccgttaat aaacctgcta
    10381 taggaaatac cgttggccct ggctgcggca ttaattctgg caatccacag cttcctaaaa
    10441 tccctctttt taagttttct acccctatag gaatgagcca acgcctttaa aacagcctgg
    10501 ttagcaggtc tgtataattt gctctgtgat cctctatatc cctttgccag tttaataatc
    10561 tttttgtgtc gcttacgtgc agtaactcct cttttaactc gtggcataat tatcctcctt
    10621 taacttacat atatggtata acttgtttta tcctcttttg gtcggctttg ctcagcacag
    10681 ccggtttcct caagtgtctt ttcctcttag cagttttttt ggtcaatata tgacttttaa
    10741 aggctttagc ccttttaatc ttcccggttt ttgaaacttt aaacctttta gctgctcctc
    10801 tgtgtgtttt tacttttggc ataaatgtca ctccttagtt tttgtttgtt ttggtgaaag
    10861 aaccatgacc atatttctgc cttccatttt aggtggcttc tcaacgactc caagctcttg
    10921 tgtcagctct atcattttgt ttaaaacttc ttcaccaagc tgtgtgtggg tcatttgcct
    10981 gccgcgaaat ctaatgctga ccttaacctt gttcccctct tttaaaaact ttaaggtatt
    11041 ttttaatcta acttggaaat cgtgctcatc aattgtggga ttcatccgga tctcttttat
    11101 attgataact ttttgatttt tgcgtgcttc tttttcacgt ttgctctgtt catatttata
    11161 tttcccaaag tccatgattt tacataccgg tggtttggca tttgaagcaa cctttacgag
    11221 atccagttgg gcctcctgag catggcgaag cgcttccttt agcggcataa tccccaactg
    11281 ctttccatca ggacctatca atcttacttc tcgagcttta atttcatggt tcacctgtat
    11341 gtctttatct ttgctaatac tgttcacctc caaaaataat gaataaaaaa gggacgagaa
    11401 gaatcactcg tcccgtcctt acgaaatcta caggtaagaa ccttataagc cttggccata
    11461 aggtgagaag tgaatcttct acttaataaa catttcattt tttcacaagt aactatacca
    11521 tatgttagcc cttactgtca attaaatttt gcagttaatt cctttacttc ctcaatgaaa
    11581 gattctatag actgttgccc tgtatcgcct ttttctctca cccgcaccga aacggtgttt
    11641 tcttccgctt ccttatcgcc aacaatgagc atgtaaggag tcttttgcat ctgtgcttcc
    11701 cgtattttgt aacctatctt ttcatttctc tcgtcaagtt ctactctgat gccggcctcc
    11761 tccagctgct gttttacttt gcgggcatat tctaagtgct tttcactgat aggcagaatt
    11821 ctcgcctgaa ccggcgcgag ccacatcggg aaagcacctc cataatgctc aatcagtata
    11881 cctataaagc gctctatgct gccgtagata acgcggtgaa tcataactgg ccggtgcttt
    11941 tcaccatctg gcccgatata ggataggtca aagcgctcgg gcatctggaa atccagttga
    12001 atggtaccgc actgccaggt tcttcctaaa ctgtccttta agtggaagtc gattttggga
    12061 ccgtaaaagg cgccatctcc ttcgtttatt ttatagggca tacctttttt ctcaagagcc
    12121 ttttgcagtg cattggtagc ctgctcccaa agttcatcgg aacccatcga attttccggt
    12181 ctcgtggaaa gctccacatg gtactcaaag ccaaatattt tgtagaagta atcaactagg
    12241 tctattacac ctattatctc ttcttctatt tgagagggca tcatgaagat gtgtgcatca
    12301 tcctgagtaa agcagcggac tctcatgagg ccatgcaata ccccggattt ttcgtgccgg
    12361 tggacgatcc ccaactctcc caatcttatg ggcaaatccc tgtaactgtg agagttgctt
    12421 ttgtacatga gtatgccccc cggacagttc atgggcttta ctgcataagc catatcatct
    12481 attttcgtaa agtacatgtt gtctttgtaa tgctcccagt ggcctgacct ttgccagagc
    12541 tcttcattta atattatagg cgttttaatt tcttgatagc cccttttctg atgttccttg
    12601 cgccaaaaat ctatcagtat attccagagc accatgccct tgggatggaa gaagggaaaa
    12661 ccaggtccct cttcatgaag gctgaaaagg tcaagttccc tgcccagttt gcggtggtcg
    12721 cgctttttgg cttcctcgat tctatgcagg tattcatcca gcatggactt tttaggaaat
    12781 gatatgccgt aaattctctg gagcatcttg ttgtcttcat cgcccctcca gtaagcaccg
    12841 gcaaggcttg tcaacttaaa ggcctttatt ttgcccgtag aggggatgtg gggtcctgca
    12901 cataggtcgg aaaactctcc ttgattatag aaactaatat ttgcatcgtc ttcaagggct
    12961 tgaaccagct ctactttata aggctcattt tgttcctcca agtcgctaag agcttcgtct
    13021 cttgaaacat cttttctgga aaaagagtaa ttttctgcaa tgattttggc catttctttc
    13081 tctatttgct caatatccga tggtgaaaag ctttcttcca tgtcaaaatc ataataaaaa
    13141 ccttcgtcaa tagcaggccc gatggcaagt tttgcgtccg ggaacaggcg ctttaccgct
    13201 tgtgccatca cgtgagatgt gctgtgccaa aatactttac gcccttcttc atcgtcgaag
    13261 gtcagaatct caactacact gtcatcttga actttataac ttaaatctct taggactccg
    13321 tctactttgg ctaccagggc ctctttggcc agttttttgc ttatatcttc agcaatttca
    13381 agcaatgaaa tccctttcgg atatgttttt ttaactccac cttttaaggt tacctgaatt
    13441 tcgctcataa caaatccctt cctttaactt tatgttacat tcaaaaagcc tctcatccct
    13501 aagggacgag aggctttccc gtggttccac cctaatttga actcattagc agttaaggct
    13561 tgccgccgta aaactccgag gtggtcttcg gcttagtttt cccagggctt tcccagctac
    13621 cggcccctct ctgagggaaa atcataagct tactcttctc ttcatagttt tctttgacta
    13681 tagaatttta tttattataa atcttatggt ggcttatgtc aagggcctga attgctcccc
    13741 tgctactg
//
LOCUS       DAONUO010000009        12440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_3900_length_12440_cov_3.939281, whole genome shotgun sequence.
ACCESSION   DAONUO010000009 DAONUO010000000
VERSION     DAONUO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12440
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3900_length_12440_cov_3.939281"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /gene="cooS"
                     /locus_tag="PK768_00735"
     CDS             <1..1273
                     /gene="cooS"
                     /locus_tag="PK768_00735"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HQA59527.1"
                     /translation="TAATRAALSDGWGGSMIGTELSDIIFGVPEPHEIEANLGVLKEN
                     EVNIVIHGHEPTLSEAILVAVNDKELIELAKKQDAEGINLVGMCCTGNEIAMRHNVPM
                     AGNFFQQELAIVTGVVDAMVVDVQCIMPALSQVAKCYHTKLITTSPKAKIPGAIHMQF
                     DEAKAIESAKKIVREAVLNFKNRTGNTFIPEEKSKGLVGFSNESIISALDGVTNSFAH
                     PQGTVKPLVDAIKSGVLRGAVAIVGCNNHKVPHDKGHVELAKELIKNDVLVVTTGCGA
                     YACIKAGLMTKEAAKLCGQGLATVCELVKIPPVLHMGSCVDISRILLLLSEVAKFMDV
                     DIDDLPVAGAAPEWMSEKALSIGTYVVASGVYTMLGVAPQVTGSENLTKFLTEDVEAI
                     YGGKFDIESDPKEAAKKIIEHIETKRKALGI"
     gene            1305..2084
                     /locus_tag="PK768_00740"
     CDS             1305..2084
                     /locus_tag="PK768_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HQA59528.1"
                     /translation="MKIAITGKGGVGKTTFAGVLSKVLAKDGYKVLAVDADPDANLSM
                     ALGLPGEKISSITPLSEIKDLIAQRTEARPGVTGQMFKLNPKVDDIPEKYCVEHDGVK
                     LLVMGTVKAGGSGCICPEHAFLRALTQHLLLDSQEALIMDMEAGIEHLGRATAGCVDA
                     FIVVVEPGQRSIQTLKTIKKLAGDLGITKIFAVGNKIRNQEEREFLIKACEDVELLGF
                     LSYDPELIEADRQGQTPFKRCILYTQQVEAIKDNILKLLDN"
     gene            2201..3880
                     /locus_tag="PK768_00745"
     CDS             2201..3880
                     /locus_tag="PK768_00745"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQA59529.1"
                     /translation="MAFKSDIEIAQEAKLEHIKDVAAKIGLTEDDLEYYGRYKAKIDY
                     NLLERYKDKQDAKLILTTAINPTPAGEGKTTTTVGLGDALRKLGKNAMIALREPSLGP
                     VFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMVDNHIYQGNELNIDP
                     RRITWKRCVDMNDRQLRFVVDGLGGKANGVPREDGYDITVASEIMAVFCLASDIEDLK
                     NRLARIIVGYTYDGKPVTAGQLKAHGAMAALLKDAFKPNLVQTLEGTPAFVHGGPFAN
                     IAHGCNSIIATKMAMKLADYVVTEAGFGADLGAEKFLDIKCRMADLKPDAVVIVATIR
                     ALKYNGGVSKENLNEENLDALKKGLPNLLKHVENVTEKFGIPAVVAINQFPTDTEKEL
                     ALVQEECKRLGVNAVLSEVWAKGGEGGLELAKEVVRIIEEGKNNYRPIYDLDMGLKDK
                     IITIAKEIYGADGVEFAPAALKEIKTLEELGFRNVPICVAKTQYSLTDDPKLLGRPTG
                     FKINVRNVRISAGAGFAVVLTGDIMTMPGLPKRPAAENIDVDANGRITGLF"
     gene            4078..4902
                     /locus_tag="PK768_00750"
     CDS             4078..4902
                     /locus_tag="PK768_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 family protein"
                     /protein_id="HQA59530.1"
                     /translation="MEHLKLFIAASKDTPDLPGFFQVKYGGEFAARKFLPHLTNFKYN
                     PHLCTKSCFEFRQRFPLDYTEYLAGIMLFPSVLPELIDDAESYVKEPIPTHDVFVAVG
                     VHPDILIELIKRVSDAGCKAVVVPREDPAWLTTSLVDKLKGLCEEKGLEYAFPRPFCS
                     LSKGKFEYINNFIDQFKIGKPQYRLVTDNKGNVRDVDVKCSSPCGATYHVASGLIGLN
                     KEDIVDTANKLWHTYPCLSSSQMDPNINDSPMHLAAYINLYAARDAQKNSEEGSQC"
     gene            4896..5525
                     /locus_tag="PK768_00755"
     CDS             4896..5525
                     /locus_tag="PK768_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HQA59531.1"
                     /translation="MLIEKSCKDFVEVLASKEPVPGGGGAAALVGAIGMALGNMVGNL
                     TVGKKKYKDVEAEVYEIMEKATKLQQDLLSMVDGDAEVFGEVAKVYRMPKNTDEEKAA
                     REEAMEKALKHACSVPMDIIKAALEAIKLQARLGEIGSIMALSDVGVGVLCLKAALIS
                     GKLNVIINLNGIKDESFVSKVSEEMDALVEEGIKIVDETYAKVEERLKN"
     gene            5539..6393
                     /locus_tag="PK768_00760"
     CDS             5539..6393
                     /locus_tag="PK768_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HQA59532.1"
                     /translation="MTKILSGKEVAASLKEKLVAEVEKLKESGKAPSLCIVMAGDRPD
                     SASYIKGAVKRCTDIGIDCHVEKLPEEISQDEFLEVLDRLNNDSKIHGIIIMRPLPEQ
                     ISEDVVKYKIAPEKDVDCFNPINVSKVMSGDSDGFPPCTPQAVIEILDHYGIEIKGKR
                     AVVIGRSMVVGRPLAMMLLKRHATVTVCHTRTKDLAGEAKRAEILIAAAGKARMVTRD
                     MLSEGAVVIDVGINFDENGKLCGDVDFDDVSEIAGQITPVPGGVGSVTSTVLAKHVVE
                     AFKQQEGL"
     gene            6406..7053
                     /locus_tag="PK768_00765"
     CDS             6406..7053
                     /locus_tag="PK768_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQA59533.1"
                     /translation="MIISKQKPFEEILNFLKGKDKIFITGCSLCATSCMTGGQQQIDE
                     MKNKLEEHGKTVTGTTVLDPSCHRLQVRATLKKQKVELASADAVLCMACGDGAQTVAA
                     FAKIPVYPANDTLFIGEVERIGHFHEACKACGQCELGWTAGICPITKCAKSLLNGPCG
                     GATNGKCEVDPDNDCAWVLIYNRLKEQDMLDTLLEIKGPKDYSKAAHPRKYVVGR"
     gene            7066..7938
                     /locus_tag="PK768_00770"
     CDS             7066..7938
                     /locus_tag="PK768_00770"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQA59534.1"
                     /translation="MNLKEKLARGDFVITAEMAPPKGIDFGHIRECARAVKGRVDGVN
                     VTDFQSAVVRASSLGGAKMLLDEGLEPIMQITGRDRNRIAIQGELLSAAALGIKNVLA
                     LTGDHPSIGDHPDARGVFDMDSVNILQAATKLMEGQDLNGNELSGTPEYFLGASVTPL
                     YDPLELQIIKMKKKIQAGAKFFQTQAVYDIDVIRSFMDEIKDLNTKVLVGVIPLKSAG
                     MAKYMNNNVPGIQVPDEIIERMRKAEDKTKEGLNIAAEFIEQIKEEKICDGVHIMAIG
                     AEENVPTLLDMCGF"
     gene            7957..9339
                     /gene="lpdA"
                     /locus_tag="PK768_00775"
     CDS             7957..9339
                     /gene="lpdA"
                     /locus_tag="PK768_00775"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQA59535.1"
                     /translation="MTKRIAILGAGPGGYVGAIKAAKMGAQVTLIEKDTVGGTCLNRG
                     CIPTKALLASSDVLTKIRQAKDFGILVDGDIRPDIDFIMDRKDKVVERLVQGIEFLLD
                     KNNVRLVKGTGKITDRNKVEVTKEDGSKEDVEADAIIIATGSSPAQIPIFPYDGERVI
                     TSDEALNLRQIPKSMIIVGAGVIGCEFAMFFNNLGTSITIVEMMDHALPLEDKEIGSE
                     MAKAMKRKKIKLMLKTKIESAEKTDTGVKAILDNGKEIEADIMLVAIGRKANIADIGL
                     EQLGVATDRGRILVDEYMETNVKGIYAIGDVVPGAQLAHLASAEAECAVENIMGNNIQ
                     MDYSAVPRGVFTDPEVSGVGLTEEEAVAKGYSIKKGDFAFRGLGKAQAMGEFFGKVKV
                     IVDEQTDKLLGASIIGPNATDIIHELAVGVRYGLTAEQLGRVIHSHPTLSEAVMEALQ
                     DVNGKSAHKV"
     gene            9352..10131
                     /locus_tag="PK768_00780"
     CDS             9352..10131
                     /locus_tag="PK768_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA59536.1"
                     /translation="MAYTVAVAGKGGTGKTTLAGFLIDYLVEKSLTPVLAVDADPNAN
                     LNEVLGEDIEITLADIREDVSIKNRDGNLPGGMSKTDYINYKLQQAIIEGKGFDLLVM
                     GRPEGEGCYCFANGILREATDRLSDNYKVLVIDNEAGLEHLSRRTTKNVDIMFAVSEC
                     SKRGIQAAARVKQLIEELKLDVKELYLIVNRVPDEGLSDDIKAAIEGYGLKLAGTVPL
                     DAKVFEYDNKGIPLVKLPKDSVAVNAARQIFDEVLISHIKA"
     gene            10167..11105
                     /gene="acsD"
                     /locus_tag="PK768_00785"
     CDS             10167..11105
                     /gene="acsD"
                     /locus_tag="PK768_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HQA59537.1"
                     /translation="MAYKMPTQKYSGKINEVTIGTDLKIGGESVLPFYSFDGDIGNKP
                     AIGMEIWDVYPESWPEAVLNIFKDVVDDPVKWAKFCIENYNPDFICLKFEGANPDGLD
                     RSPEECAEVAKNVADNVNVPLVIAGCQNSEKTAKVFTKVAEALANKNFVFLSAVESNY
                     KEVGAAVGLAYGNIVAAESSVDLNLAKQLNILITQLGIQPDRMVMDPGCSAVGYGFEY
                     VVTTMDRIRLAALQQNDSTLQVPMILPVSFESWKVKESIATEDDAPEWGSQEDRGVGM
                     EISTAVGVLAAGANAVILRHPRSVEVVRNFVNQMTE"
     gene            11124..>12440
                     /gene="acsC"
                     /locus_tag="PK768_00790"
     CDS             11124..>12440
                     /gene="acsC"
                     /locus_tag="PK768_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HQA59538.1"
                     /translation="MALNGMQIYKLLPKKNCKECGFPTCLAFAMKLAQGNVEAEKCPY
                     MSDEAKAQLAEATAPPMRTVKFGAKDFEYALGGETVLFRHEKTFVNRPRFAVMFSDTM
                     SPEEVDKKINNINAVDYNRINEEMYVEIAAFRCESQDKDAFIGLIKKVMENCPKVVPM
                     IITENAEIAAAAVNECADRGPILYGANGENYEAMVNLAKDKKLLLGVTASGKESLYEL
                     VEKIQNTGYRELLLDAGSRNLKNAFDDMVNIRRTALLSGDRTFGYPTVAFVCDMAEDK
                     MMQLACATLFVQKYASIIVLSDIDYSMALPLFAHRQNIFTDPQRPMRIEQGIYKIGDA
                     NEESPLLVTVDFALTYFIVSGEVERSKVPSWVLIPDAGGYSVLTAWAAGKFNGKSIAN
                     AVKEFEVENKLKTRELIIPGKVAVLKADIEDELPGWKVVVGTEEAAT"
BASE COUNT         3887 a         2581 c         3175 g         2797 t
ORIGIN      
        1 gactgcggcg acgagagctg cactaagtga cggatggggc ggttccatga ttggaacgga
       61 actatccgac ataattttcg gcgttcccga gcctcacgaa atcgaagcaa acctcggagt
      121 tctcaaggaa aatgaagtaa acatagtaat ccacggccac gagccaacac tgtcagaggc
      181 gatacttgtt gcagtaaatg acaaagaatt aatagaactt gccaaaaaac aggacgcgga
      241 agggataaac ctggtaggca tgtgttgcac gggtaatgaa atcgccatgc gccataatgt
      301 tcccatggca ggcaacttct tccagcagga gttagcgata gtaacaggtg tcgtagacgc
      361 catggtagtc gatgttcagt gcattatgcc cgccttgtca caagtggcaa aatgctatca
      421 tacaaagctc attacaactt cacctaaagc caaaattccg ggcgccatac acatgcagtt
      481 tgacgaagca aaagcaatag aatcagccaa aaaaatcgtt cgggaagcag tgctcaattt
      541 caaaaacaga acaggaaata ccttcattcc ggaagaaaaa tcaaaaggat tagtaggctt
      601 tagcaatgaa tcaatcataa gcgccttgga tggtgtcacg aacagctttg cacatcctca
      661 gggaaccgta aaaccgttgg tagatgccat caaatccgga gtactgcgcg gagcagtagc
      721 catcgtaggt tgtaacaatc acaaggttcc ccacgataaa ggccatgtag aacttgctaa
      781 agaattaatc aaaaatgacg tattagttgt taccaccgga tgcggtgcct atgcctgcat
      841 aaaagccggc ctcatgacaa aagaagccgc aaaactgtgc ggccaaggcc ttgccaccgt
      901 ctgcgaactg gtgaaaattc caccggtgct gcacatgggc tcttgtgttg acataagcag
      961 aatactactc ctgctttcag aagtagcaaa gttcatggat gtggacatag atgatttacc
     1021 cgttgccgga gcggctcccg aatggatgtc agaaaaagct ttatctatcg gaacatatgt
     1081 tgtggcttca ggcgtatata ccatgttagg agtagcgccc caggtgaccg gttctgaaaa
     1141 cttaaccaaa ttcctcaccg aggatgtaga agctatctat ggcggaaaat ttgacataga
     1201 atcagacccg aaagaagccg ccaaaaagat tattgagcat attgaaacaa aacgaaaagc
     1261 cttgggaatt taacaagccc caggatatta aggatgtgtt gcccatgaaa atagccatta
     1321 ccggcaaagg tggcgttggc aagaccacct ttgctggagt cctttccaaa gtactagcaa
     1381 aagatggata caaagtgctt gccgttgatg cagaccccga tgcgaatctt tccatggccc
     1441 taggcttgcc tggtgaaaaa atatcatcga taactccact ctctgaaata aaggacttaa
     1501 tagcacaaag aacagaagcc cgaccgggag tgaccggcca gatgttcaag ttgaacccaa
     1561 aggtcgacga catacctgaa aagtactgtg tcgaacacga cggagtaaaa cttttggtca
     1621 tgggcacggt taaagcagga ggttcaggct gtatatgccc cgagcacgcc tttttgcgag
     1681 cactgaccca gcacctgcta ttagattcac aggaagcttt aataatggat atggaagcgg
     1741 gaatcgagca tctgggaaga gccactgcag gctgtgtcga tgctttcatt gtagttgtag
     1801 aacccggcca aagaagcatc cagaccctga aaactataaa aaagttggct ggagaccttg
     1861 gaatcacgaa aatttttgct gtaggaaata agatcaggaa tcaagaggaa agggagtttt
     1921 taatcaaagc ctgtgaagat gttgagcttt taggttttct ctcctatgat ccggaactaa
     1981 tagaagctga taggcaaggc cagactcctt ttaaaagatg tatactttat actcaacagg
     2041 tggaagcaat taaggacaat atactgaagt tgttggacaa ttgattttgg cacaaagctc
     2101 tggacagaag aatggcaaat cagctcctta gaaatccacc ctcatcttct atcagagcta
     2161 cttttaaaaa acagcaaata tattcaaggg aggaagaaaa atggctttta agagcgacat
     2221 tgaaattgcc caagaagcta aattagagca catcaaggat gtagccgcta aaattggcct
     2281 aactgaagac gacctggaat actacggcag atacaaggcc aaaatcgact acaacctgct
     2341 agagcggtac aaggacaagc aagatgccaa gctcatattg acgacggcta tcaatcctac
     2401 acctgccgga gaaggcaaaa ccaccactac cgtcggctta ggcgatgcat taagaaagct
     2461 tggtaaaaat gccatgattg cccttagaga accttcgctt ggccccgtct ttggagtaaa
     2521 aggcggcgca gcagggggcg gctatgccca ggtagttccc atggaagaca taaaccttca
     2581 ttttacgggg gatttccatg ctataggtgc agccaacaat ctcttggcgg ccatggttga
     2641 caaccacatt taccagggaa atgagctaaa catcgatccc cgccgcatta cctggaagag
     2701 atgtgtggac atgaatgacc gccagctgag gttcgtggta gacggccttg gcggaaaagc
     2761 caacggagtg cccagggaag acgggtatga cataactgta gcttctgaaa taatggcagt
     2821 attttgcctg gccagcgata ttgaagactt gaaaaatcgc cttgcacgaa taatcgtagg
     2881 ttatacgtat gacggcaaac ccgttacagc agggcaattg aaagcccatg gcgctatggc
     2941 ggcactgctt aaagacgcct tcaagcctaa cctggtgcag accctagagg gaacgccggc
     3001 attcgtacac ggagggcctt ttgccaatat cgctcacggc tgcaacagca tcatcgccac
     3061 aaaaatggcc atgaagctgg cggattacgt agtgacggaa gcaggttttg gtgcagactt
     3121 aggtgctgaa aaatttttgg atataaaatg tcgcatggca gacctgaaac cggatgcagt
     3181 tgtgatagtt gcaactattc gcgcattgaa atataatgga ggagtcagta aagaaaatct
     3241 aaatgaggaa aacctggatg cactaaagaa gggattacca aacctattga aacacgtgga
     3301 gaacgtaacg gaaaaattcg gcattccggc agttgtagcc attaaccagt tcccgactga
     3361 taccgagaaa gaacttgccc ttgttcagga agagtgcaag cgcttaggcg taaatgccgt
     3421 gctgtccgaa gtttgggcaa agggcggcga aggtggcctt gagcttgcaa aagaagtcgt
     3481 gagaatcatc gaagaaggca aaaacaacta taggcccatc tacgacttgg acatgggttt
     3541 gaaagataaa ataattacca ttgccaagga aatatacggc gcagacggag tagaatttgc
     3601 gcccgcagca ttgaaagaaa taaaaacctt ggaagaattg ggattcagga atgtgcccat
     3661 ctgcgttgca aaaacccagt attcattgac cgatgacccc aaactgttgg gtcgacccac
     3721 cggctttaag ataaatgtaa gaaatgtgag aatatccgcc ggtgccggct ttgccgtagt
     3781 gcttaccggt gatattatga caatgcccgg ccttccaaaa cgcccggcag cagaaaacat
     3841 tgatgtagat gcaaacggaa gaataacagg actattttag aaattaaagt tccaacaaaa
     3901 ggtcgtcatg taattccgct catgggttta catgacgacc aaaaacagat attattatat
     3961 tatagattgt ctagacatgt atataattat gagaaagacc taacaatcac tatcggtaaa
     4021 aatgttcaaa ttgatcttta ttcggcaaat caaaacagac ttaaaggtgg tcttcccgtg
     4081 gaacatctaa aacttttcat tgcagccagc aaagacacac cggacctgcc aggcttcttt
     4141 caggtcaaat acggaggaga attcgctgcc cgaaagttct taccccatct tacaaacttt
     4201 aaatacaatc cccacttgtg cactaaatcc tgctttgaat tcaggcaaag attcccgctg
     4261 gattatacgg aatatttagc aggcatcatg ctttttccct ctgtcctccc tgaacttatt
     4321 gatgatgccg aatcatatgt aaaggaacct atcccaactc atgatgtgtt cgtagccgta
     4381 ggtgtgcatc cggacatact cattgaactc ataaagcgag tttcggatgc cggctgcaaa
     4441 gcggtggtag ttcctcggga agacccggct tggctgacta catccctggt tgataaatta
     4501 aagggcctgt gtgaagaaaa aggactagaa tatgctttcc ctcggccatt ctgttcactt
     4561 tcgaaaggaa agtttgaata tataaacaat ttcatagatc aattcaagat aggaaaaccg
     4621 caatacaggc ttgtaaccga taacaagggg aatgtaagag atgtagatgt taaatgttca
     4681 tctccctgcg gagctaccta tcatgtagct tcggggctta tagggttaaa taaggaagac
     4741 atagtcgata cggccaacaa gttgtggcat acctatcctt gcctttctag cagtcaaatg
     4801 gaccctaata taaacgactc gcctatgcac ttagcggcat acatcaactt atatgcggca
     4861 agggatgctc aaaaaaactc tgaggaggga agccagtgtt aatcgaaaaa agctgtaagg
     4921 attttgtaga ggtcttggcc tctaaggaac cggtccccgg aggaggaggc gctgcggcct
     4981 tagttggtgc cataggcatg gccttaggca acatggtagg caacttaact gttggtaaaa
     5041 agaagtataa agacgtggaa gcagaagtct atgagatcat ggaaaaggcg acaaagttac
     5101 agcaagattt actgtcaatg gtagacggcg atgccgaggt ttttggcgaa gtggccaagg
     5161 tataccgcat gccaaagaat acggatgaag aaaaagctgc tcgggaagag gctatggaaa
     5221 aggcgttaaa acatgcctgc agcgtgccca tggacattat caaagcggcc ttggaagcta
     5281 ttaagcttca ggcacggctt ggagaaatag gttccatcat ggctttaagt gacgtgggcg
     5341 tgggagtgct gtgtttgaaa gctgccttaa ttagcggaaa gctgaatgta ataataaatt
     5401 tgaacggaat caaagatgag agctttgtct caaaggtcag cgaggaaatg gatgccttgg
     5461 tggaagaggg catcaagatt gtcgatgaaa cttacgcaaa agttgaagaa cggcttaaaa
     5521 attaaggggg tttggcaaat gactaaaata ctcagcggca aagaagtagc ggcatcgtta
     5581 aaggaaaagc ttgtggccga agttgaaaag ctcaaggaat caggcaaggc tcccagcctt
     5641 tgcatagtga tggcaggaga tcggcccgat tcagcatcgt acatcaaagg tgccgttaag
     5701 cggtgcaccg atatcggcat agactgtcat gtggaaaaac ttcctgaaga aatcagtcag
     5761 gatgagtttt tggaagtcct tgacaggttg aacaatgatt caaagataca cggtattatc
     5821 attatgaggc cgcttccgga gcagatttcg gaagatgttg tcaagtataa aatagctcct
     5881 gaaaaggacg tggactgctt taatcccatc aatgtcagta aagtgatgag cggcgacagc
     5941 gatgggtttc ctccatgtac acctcaagcg gtaattgaga ttttagacca ttacggaatc
     6001 gaaatcaagg gtaaaagggc agtggttatt ggaaggagca tggttgtagg caggcctttg
     6061 gcgatgatgc tactcaagcg ccatgctacc gtgacagtat gccacaccag gaccaaagac
     6121 ctggccggtg aagccaagag agccgagata ctcattgcag cggcaggaaa ggctcgaatg
     6181 gttacaaggg atatgcttag cgaaggggca gttgtaatag acgttggaat taactttgat
     6241 gaaaatggga agctctgcgg cgatgtggac tttgacgatg taagtgaaat cgcgggccag
     6301 attactcccg tccccggagg tgttggttct gtaacatcca cggtacttgc caagcacgta
     6361 gtcgaagcat ttaaacaaca agaggggcta taggaggaaa attttatgat tatatccaaa
     6421 caaaaacctt ttgaggaaat actaaatttt ttgaagggta aagataaaat tttcataacg
     6481 ggttgcagct tgtgtgccac atcctgtatg acaggtggcc agcagcagat cgatgaaatg
     6541 aaaaacaagc ttgaagaaca cggcaaaacc gtaaccggaa ccacagtttt agacccgtcc
     6601 tgtcacagat tgcaggttag agcaactctc aaaaaacaaa aggtcgaact agcaagtgcc
     6661 gatgcggttt tatgcatggc ctgcggagac ggtgcccaga cggtggcagc ttttgcgaaa
     6721 atcccggtat accctgccaa tgacaccctg ttcataggtg aagtagagag aataggccat
     6781 tttcacgagg cttgcaaagc ctgcggccag tgcgaacttg gatggactgc cggcatttgc
     6841 cccataacaa agtgcgccaa gagcttgcta aatggcccat gcggtggagc aacaaacggc
     6901 aaatgcgagg tggatccaga caatgactgt gcatgggttt taatatacaa ccgactgaaa
     6961 gaacaagata tgcttgacac actgctggag ataaagggcc ccaaggatta cagcaaggcg
     7021 gcacatccca gaaagtatgt cgtcggtaga taggaggaag gtcggatgaa tctgaaggaa
     7081 aaattggcaa gaggagactt tgttatcact gctgaaatgg ctccaccaaa aggcattgac
     7141 tttggccaca ttagggagtg tgccagggcg gtcaagggaa gggtagatgg agtaaatgtc
     7201 acggattttc aaagtgccgt agtgagggct tcttccctgg gaggagcaaa aatgctgctg
     7261 gatgagggcc tggaaccgat tatgcagatt accggcaggg acagaaaccg catagctatt
     7321 cagggcgagc tgctttcagc ggcagctttg ggcatcaaaa atgtattggc actgaccgga
     7381 gaccatccca gcatagggga ccatccggat gcccgcggag tatttgacat ggattcggta
     7441 aatattcttc aagcggcaac aaaacttatg gagggacagg atttaaacgg caatgagctt
     7501 tcagggacac ccgagtactt cttgggagcc agtgtgacac ctttatacga tcccttagag
     7561 ctgcagataa ttaagatgaa gaaaaaaata caggcgggtg cgaagttttt ccaaacccag
     7621 gctgtttatg atatagatgt cattaggagt ttcatggacg aaataaagga tctaaacacc
     7681 aaagtgctgg taggagtcat accgctaaaa tctgccggta tggcaaaata tatgaacaac
     7741 aacgttccgg gtatccaggt gcctgatgag ataattgaga gaatgagaaa agctgaagac
     7801 aagacaaaag aagggctaaa tatcgcagct gaatttatcg aacaaataaa agaagagaaa
     7861 atatgcgacg gagtccacat catggccatt ggggcagagg aaaatgtccc cacacttctt
     7921 gacatgtgtg gattctagat gacaggaggc ttggtggtga ctaaaagaat agcaatatta
     7981 ggtgctggcc ccggtggata cgtgggggcc atcaaagcgg caaaaatggg agctcaggtt
     8041 acactaattg aaaaggacac ggtaggaggg acctgtctta ataggggctg tattccaaca
     8101 aaggcgctcc ttgcatcaag cgatgttttg acaaagataa gacaggccaa ggattttgga
     8161 attttagtag atggtgatat caggcccgac atcgatttca taatggaccg gaaggataaa
     8221 gtagtggagc gcttggtcca gggcatagag tttttgctcg acaaaaacaa tgtcaggctt
     8281 gtcaaaggca caggcaaaat aacagacagg aataaagttg aagtgactaa agaagatggc
     8341 agcaaagagg acgttgaagc ggatgccata attattgcca ccggctcatc acctgcccaa
     8401 attcccatct tcccctatga tggcgaaagg gttattacaa gtgatgaagc gctaaatttg
     8461 aggcagattc caaagagcat gatcattgtt ggagccgggg ttatcggatg cgaatttgca
     8521 atgtttttca acaacctggg cacttccatt accatagtgg aaatgatgga tcatgccctg
     8581 cccctggaag ataaggaaat cggcagtgaa atggcaaaag cgatgaagcg caagaagata
     8641 aagctaatgc tgaagaccaa gattgaaagt gccgaaaaaa ccgataccgg cgttaaggct
     8701 attctcgata acgggaaaga aatcgaggca gatataatgc tggttgctat cggccgcaag
     8761 gccaatattg ccgatatagg ccttgagcag ttgggagttg ccactgatag aggcaggata
     8821 cttgtcgatg aatatatgga gacaaatgtg aaaggaattt acgcaatcgg agatgtggtg
     8881 ccaggagcac aacttgccca cctagcctca gccgaggcag aatgcgctgt ggaaaatatc
     8941 atgggaaaca atatccaaat ggattactct gcggtcccta gaggagtgtt tacagacccc
     9001 gaggtatcgg gagtggggct tactgaagaa gaagcagttg ccaagggtta cagcattaaa
     9061 aaaggagatt ttgcctttag aggtctcgga aaagcccagg caatgggaga gtttttcggc
     9121 aaggttaaag tcatcgtcga tgagcaaaca gacaaattac tgggagcttc aatcatcggg
     9181 cccaatgcga cggatatcat ccacgaactt gcagtaggcg taagatatgg acttacagca
     9241 gagcagctcg gcagagtcat tcactcacat cccacattaa gcgaagcggt aatggaagcc
     9301 cttcaagatg taaacggcaa atcagcccat aaagtataag gagatgatag tatggcttac
     9361 actgtagccg ttgccggaaa aggcggaacc ggcaaaacca ccctggcagg ttttttaatc
     9421 gattacctgg tagaaaaaag tttaacaccg gtcctggcgg ttgacgcaga ccccaatgcc
     9481 aatttaaatg aagtcttagg tgaagacatc gagataacct tggcagatat cagagaagat
     9541 gtgagcataa agaatcgcga cgggaatttg ccgggtggca tgagcaaaac tgactatatt
     9601 aattacaaac tccagcaggc cataattgaa ggcaagggat ttgacttatt ggtcatgggg
     9661 aggccggaag gagaaggttg ctactgcttt gccaacggca ttttgagaga agcaaccgac
     9721 aggctctcag acaactacaa agtattagtc atcgacaatg aagcaggcct tgagcacctg
     9781 agccggcgaa ccaccaagaa cgtggacata atgtttgcag tgagcgaatg ctccaagcgt
     9841 ggaatccagg cggcggctag agtgaagcag ctcatcgaag aattaaagct agatgtgaag
     9901 gaactttacc tcatagtaaa ccgggtccct gacgaagggc tgtccgatga cataaaggca
     9961 gctatagaag gctacggctt aaaattagcc ggcactgttc cccttgacgc gaaagtcttc
    10021 gagtatgaca acaagggcat accccttgtc aagctgccca aagactccgt ggcagtaaat
    10081 gccgcaaggc aaatttttga cgaggtgtta atttcccata taaaggctta aagcccatat
    10141 gtcaataaat aattggagga gatactatgg cttataaaat gccaacacaa aagtattctg
    10201 gcaaaatcaa tgaagttaca attggaaccg acctaaaaat aggcggcgaa tcggtccttc
    10261 ccttttacag ctttgacggc gatatcggaa acaaaccggc catcggcatg gaaatatggg
    10321 atgtatatcc agaatcctgg cctgaagctg tgctaaacat tttcaaagat gtggtcgatg
    10381 acccggtaaa atgggccaag ttttgcatag aaaactacaa tcccgatttt atatgcctga
    10441 agtttgaagg tgcaaatcct gacggccttg ataggtcacc ggaagaatgc gccgaggttg
    10501 cgaaaaatgt agccgacaat gtcaatgtac ccctggttat tgcgggctgt caaaacagcg
    10561 aaaagaccgc caaagtcttt acaaaagtcg ctgaagcatt ggccaataaa aacttcgtat
    10621 tcctgtctgc tgtagaatcc aattataaag aagtgggagc ggcggttggc cttgcctatg
    10681 gcaacatagt cgctgccgaa tcctccgtcg acctaaacct tgcaaagcag ctaaacatac
    10741 taatcaccca gcttggcatc cagccggata gaatggtcat ggatcccgga tgttcggcgg
    10801 tgggatatgg ctttgagtac gtagttacca ctatggacag gataagactc gcagctctcc
    10861 agcaaaatga cagcaccctg caggtgccaa tgattttacc cgtatccttt gaaagctgga
    10921 aggtaaagga atctatcgcc acggaagacg atgcgcccga atggggcagc caggaagaca
    10981 ggggcgtcgg catggagatt tccactgcag ttggagttct cgcagccggt gccaatgcgg
    11041 taattctcag gcatccccgt tcagttgaag tagttcgcaa ttttgtaaac cagatgaccg
    11101 aataagtaaa ggagagatga attatggccc tcaatggcat gcaaatatat aaattactgc
    11161 caaagaaaaa ctgtaaagag tgcggattcc ccacctgcct ggcctttgcc atgaaactgg
    11221 cacaggggaa cgtggaggcg gaaaagtgcc cttacatgtc ggatgaagcc aaggcacagc
    11281 ttgctgaagc cacagcacct cccatgagaa ccgtaaaatt cggtgcaaaa gatttcgaat
    11341 atgctcttgg cggagagacg gtgcttttca ggcatgaaaa aacctttgtc aaccgcccca
    11401 gatttgcggt aatgttttca gacacaatga gccctgaaga agttgataaa aagatcaata
    11461 acatcaatgc cgtagactat aacaggatca acgaagaaat gtacgtggaa atagctgcat
    11521 tcaggtgcga gagccaggac aaagatgcat ttatcggcct cattaaaaaa gtcatggaaa
    11581 attgtcccaa ggtagtaccc atgatcatca ctgaaaatgc agagattgca gccgcagcag
    11641 taaatgagtg tgcagataga ggtcccatac tctatggtgc caatggggaa aactacgagg
    11701 cgatggtaaa cttggcaaag gataagaagc tgctgctcgg agttaccgca tcaggtaaag
    11761 aaagcttgta tgaactggta gagaagattc aaaataccgg ctatagggaa ttgttgctgg
    11821 atgcaggcag ccgcaacttg aaaaatgcct ttgatgatat ggtaaacata agaaggactg
    11881 cccttttgtc cggcgacagg accttcggat atcccacagt tgcctttgtc tgcgatatgg
    11941 cagaagataa gatgatgcag ctggcctgcg cgacactctt tgttcaaaaa tatgcgtcta
    12001 taatcgtctt aagcgatatc gattactcaa tggcattgcc actgttcgct cacaggcaaa
    12061 acatctttac agatcctcaa agaccgatgc ggatagaaca gggcatatac aagataggtg
    12121 acgccaatga agaatcaccg cttcttgtta ctgttgactt tgcattgacc tatttcatag
    12181 tttcgggaga agttgaaagg tcgaaggtgc cttcttgggt cttgataccc gatgcaggtg
    12241 gatactcggt gcttactgct tgggctgccg gcaagtttaa cggcaaatca atagctaatg
    12301 cggtaaaaga atttgaagta gaaaacaaac taaagaccag agaacttata atccccggca
    12361 aagtagccgt gctcaaagcc gatatagaag atgagctgcc gggctggaaa gttgtggttg
    12421 gcaccgaaga agctgccacc
//
LOCUS       DAONUO010000010        12382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_3926_length_12382_cov_4.328385, whole genome shotgun sequence.
ACCESSION   DAONUO010000010 DAONUO010000000
VERSION     DAONUO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12382
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3926_length_12382_cov_4.328385"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1411
                     /gene="gatB"
                     /locus_tag="PK768_00795"
     CDS             2..1411
                     /gene="gatB"
                     /locus_tag="PK768_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQA59539.1"
                     /translation="MVGLEVHVELSTKTKIFCGCSTEFGKEPNSHCCPICLGMPGTLP
                     LMNKSVVEYATKAGLALNCEIAEFSRFDRKNYFYPDLPKGYQISQYYIPLARNGYVEI
                     ESNGEKKKIGIRIMHMEEDAGKLIHEEDGSHSLVDLDRAGVPLIEIVSEPDIRSGEEA
                     WLYLNNLKTILQYIEVSDCKIEEGSMRCDINISLRPEGSDEFGTKVEVKNLGSFRAVR
                     RAIEYEERRQREILESGGKVLQETRRWDEARGITIHMRGKVENRYSLEPNLAPVIMNK
                     EWKEEIFRQIPELPDAKKARFIEEYGLPEYDASVITASKILSNFYEECVKHYHDPKII
                     SNWLMVELLSILNETGQGIKDLKFTPKQLCDMLAMIDKGTISGKIAKDVFREMFDTGK
                     SPEAIVKEKGLIQISDESQLESIAKQVIENNPKSVEDYKNGKKKALGFLVGQVMKETK
                     GKANPQMVNEIFIRLLNDE"
     gene            1605..2855
                     /locus_tag="PK768_00800"
     CDS             1605..2855
                     /locus_tag="PK768_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017560897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HQA59540.1"
                     /translation="MAVIKAFKALRPVPHLVSKVAALPYDVVTTKEAAEIARDNRYSF
                     LRIDRPEIAFDDSMDPYDTKVYEKAREILFSMIDEKIYIQDDKPCLYIYQLNRQNRIQ
                     KGLVACASIDDYLNDVIKKHEHTLPVKEQDRMNHVNYTEAHTGPILMSYRENVRITEI
                     IDNWTSSHNPIYDFVSMDQVGQTVWIIDDPDTIEEIVNLFAGIDALYIADGHHRAAAA
                     VKVGLAKKRENPDYTGKEEFNYFLTVIFPDTQLTILAYNRVIKDLNGLSEQELLEKIK
                     ESFDIVYKGEEAFIPDAKHSFGMYIDKKWYGLKVKPGSFNESDPVASLDVSILHDLII
                     APVFGITNPRTDERIDFVGGAKGVEELEKRVNSGEMSVAFSLFPTPIEDLMAISDAGA
                     IMPPKSTWFEPKLQSGIFIHSMSL"
     gene            complement(2981..4702)
                     /locus_tag="PK768_00805"
     CDS             complement(2981..4702)
                     /locus_tag="PK768_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA59541.1"
                     /translation="MEKKVKIVVLGGGFGGLTAAQSLDRYLRKNRNVEIILINKTPNQ
                     VYLTQLHEIAGNRIPDDGILYSLEAALDQTRVKLVVDEINKIDVKNRRLYGVDNEYSF
                     DYLILACGSEPAFFDIPGMKENAFTLWSHEDAVKIREHIVNMFELASTEEDAEKRKEY
                     LTFVVGGGGYTGIEMIGELVEWVEDLCIDYNIKASEVSLMVVEALPTILPTMSDSLIE
                     NATNYLRKKGVQILTNARIVNVTANSLTLESGETVNSRTLIWTGGVRASSLVANSGLE
                     LGKRNKVVVNEFMESSEPGIYAIGDNTYYEDKSGNVMPCLVESAIQSAKCAAYNVAAE
                     INNKSKKPLKLKLHGNMVSIGSEYCIAEVMGFKLKGFLASAAKYLADIHYLFGISGFG
                     FIFDYIDHQFFRKTRNKPTVIKHIGVSTSAAWLALLRIFLGYKWLMSGIDKVNSGWLS
                     AGDKLVAGASTAPIGPNTPDWYAQFVESVIYPNAYLFQVMITLGEIALGICFILGLFT
                     VLAAIGSIFMNLNFIISGSGDMWFLVVSIAMLAGAGRSFGLDYYVVPYVSKIIKRFLR
                     RGEIKLT"
     gene            4921..6102
                     /locus_tag="PK768_00810"
     CDS             4921..6102
                     /locus_tag="PK768_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQA59542.1"
                     /translation="MYKKFYKSTFFAGLFVFCAEIKNEYYKRIPKKANFSGGGKGGII
                     TRLNKKVFRLIIAAFIIVACIAACSRNSGEKPISKTNFMLDTLISVQAYGPNASKAID
                     KAFERIREIETKMTSKADYSEVIGINQMAGQDFFKVSPDTYYVIKKGLYYSELSDGKF
                     DITVGPLVELWGIGTEHARVPGNDEIQEILQFVDYTQVEIKDEDNSVFLKSPGMAIDL
                     GGIAKGYAADEAADVLRQNGVEHGTVNLGGNIVVIGAKPDGRPWKIGIQNPFFKTRGS
                     VMGSVEVTDKTLVTSGPYERYLEKDGKIYHHILDTKSGFPVENELMSVTIISDSSIDA
                     DALSTAVFAMGLEDGVDFVEGLDYLDAVFITKDYKVYITAGVGRYNFTLTDDQFELME
                     R"
     gene            6245..6874
                     /locus_tag="PK768_00815"
     CDS             6245..6874
                     /locus_tag="PK768_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HQA59543.1"
                     /translation="MFKKFKIPDATIGRLSLYSRYLAEADEKGITTVSSQNIARETGV
                     TPAQVRKDLAYFGEFGTRGVGYNSRELYNYIMKILGLDKRWSVAIIGAGNLGRALTQY
                     KGFQQRGFDVKCIFDNDPKKIGKKLANVEIYPIDSLEEKVKELDINLGIIAVPAPSAQ
                     EVADKLVGAGIKGIINFAPINITVDGSVILRRVDLAAQLEYLTYYLEET"
     gene            6874..7683
                     /locus_tag="PK768_00820"
     CDS             6874..7683
                     /locus_tag="PK768_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA59544.1"
                     /translation="MKLLEIKELLQAKVLWGEEFLDIDVSNACGADLMSDILADNKFN
                     AILLTGLTHRQIIQTTVMGDFAAIVFVRGKVPPEDVIELAKAEKLPLLTTSFPLYESC
                     GILYKAGLGQDRVNGEASDRSSRGDSESPEAMRLTYDIVGNDFNSAGKAAEQAKRVLK
                     QIGIDSGVIRKVSVAAYEAEMNIVIHAYKGKLIFSITPRYIELIAEDEGPGIPDIEKA
                     MQEGYSTAPDRIRELGFGAGMGLPNMKKFSDFFEIDSELGKGTKVRMVINL"
     gene            7728..9044
                     /locus_tag="PK768_00825"
     CDS             7728..9044
                     /locus_tag="PK768_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HQA59545.1"
                     /translation="MTLDVDKCKGCTNCIKGCPTEAIRVRNGRAYILENKCIDCGECI
                     RICPNNAKYAATDSIDRLKDFKFTVALPAPSLFGQFKEGTTTKNVIYALKKIGFDEVY
                     QVPLAAEEVSIAIREYISRHKNLRPIISSSCPAVIRLIQVRFPELIKNIIPIQSPMEI
                     AAKRAKEILGEKYDLAPEEIGVFFISPCPAKATSIKQPIGTGRSYVDGVISISGIFGE
                     IVKNLDAKAFQSVFPDSSGIGIGWGRSGGENMAIGGDNYLAVDGIQNCIEVLEEVEMN
                     KLSDVDYIECQACVGGCIGGALTVENHYIARVKLRRLSEKMGYQSILDEASVIEQFNQ
                     GYYHMEGEIQAKSAFKLDDNLAEAIRKMELLEKTVKDLPGLDCGSCGSPNCRALAEDI
                     VKGLANETDCIFKLRDKVKHLAAEMFDLAQKMPPTMESEDRGNDSR"
     gene            9041..9385
                     /locus_tag="PK768_00830"
     CDS             9041..9385
                     /locus_tag="PK768_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQA59546.1"
                     /translation="MKLSEVQDLLGLKLVTKVTNLDRKVGGGYASDLLSWVMAHAKEN
                     DIWITIQSHQNIVAVASLLSLAAIIVAEDANIDENTITKAEQENIPIYSSDKSIYELC
                     GILYNLLSRESG"
     gene            9385..10131
                     /locus_tag="PK768_00835"
     CDS             9385..10131
                     /locus_tag="PK768_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA59547.1"
                     /translation="MLLEYPADLHIHTCLSPCASDEMIPVNILNMAALIGTKILGICD
                     HNSAGNIEAMLKAARDYDILVIPGIEVQSAEEVHLVCLFETINQLLQFQEFIYDALPP
                     IKNNPQFFGHQWLVDDRGNLLGEETRMLLTSASLTVDEVAQKVHELQGLIIPAHVDRR
                     HFSIIGQLGFIPENLKIDAVEFSRAISLAEFKSTFKSLTNNPLITSSDAHSLQEMVYQ
                     KTYFLLEELNFKEIILALRGLEGRKVLIKE"
     gene            10193..10681
                     /gene="nuoE"
                     /locus_tag="PK768_00840"
     CDS             10193..10681
                     /gene="nuoE"
                     /locus_tag="PK768_00840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQA59548.1"
                     /translation="MAVALNQQQDFIEKLNKIEDMLKKYQGKRSALIQALQEAQAIMG
                     YLPIEVQKLVAENLGVTLSEVYSTITFYSFFNLKPIGKYHIRVCLGTACYVRGAEKVL
                     DRLKTELGIEVGDTTDDLKFTLDGCRCIGACGLAPAIIVNDEVYGRLTPDKIPEILKK
                     FE"
     gene            10684..11232
                     /locus_tag="PK768_00845"
     CDS             10684..11232
                     /locus_tag="PK768_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA59549.1"
                     /translation="MKELSLHILDIVQNSLGAKASLIEININEDVKNDLLVIRIKDNG
                     LGMDTATAQKVMDPFYTTRTTRRVGLGIPLFAQAAESCGGDFKIYSEKGRGTEIVATF
                     TLSHIDRAPIGSMADTMVSLIAMRPDVDYIYRHCVGDREFTLDTREIKETLEDVPINN
                     PMVIDWIKEFVNGNLKEINGGA"
     gene            11234..11608
                     /locus_tag="PK768_00850"
     CDS             11234..11608
                     /locus_tag="PK768_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQA59550.1"
                     /translation="MPVIKSIEDLKKIRDQAKDQINLRVDNESKTRVVVGMGTCGIAA
                     GARQVMLAILEELKKRNVSDVIVTEAGCIGLCQYEPLVDVIKPDRPKVTYVNMNPDKA
                     REIVVKHIINDQVVDEYVVPNI"
     gene            11643..>12382
                     /locus_tag="PK768_00855"
     CDS             11643..>12382
                     /locus_tag="PK768_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQA59551.1"
                     /translation="MEIYRAHVLVCKGTGCVASGSEPIFETLQKEIEKKGLDKEVKVI
                     QTGCLGLCELGPNILIYPEGSYYCQVKAEDVPEIVEEHLLKGRVVQRLLYKEPDSKER
                     YRSLMDIDFYKRQKRIALRNCGIINPEIIEEYIANDGYAALAKALTDMKPQEVIDEVK
                     KSGLRGRGGGGFPTGIKWQFAANAEGDIKYVVCNADEGDPGAFMDRSILEGDPHSVLE
                     AMAIAGYAIGASEGYIYVRAEYPIAVKRL"
BASE COUNT         3891 a         2378 c         2868 g         3245 t
ORIGIN      
        1 cgtggtaggc ttggaagtgc atgtggaact gtcgacgaag accaagatat tttgcggctg
       61 cagtactgag tttggcaaag agcccaacag ccactgctgt cctatctgtc tcggcatgcc
      121 cggcaccctg cctctcatga ataagagcgt agttgagtat gcaacaaagg ccggccttgc
      181 tctaaattgc gagatagccg agttctcaag atttgacagg aagaattatt tctatcccga
      241 ccttccgaaa ggttaccaga tctcccaata ctatattcca ctggcgagaa acgggtacgt
      301 tgagattgaa tctaatggcg agaaaaagaa gataggtatt agaattatgc atatggaaga
      361 ggatgccggc aaactgattc atgaagaaga tggatcccat tctcttgttg acttggacag
      421 ggccggtgtt cccttaatag aaatcgtgtc agaacctgac ataaggtcgg gcgaagaggc
      481 ttggctctac ttaaataacc tcaagacgat actccaatac atcgaagtat ccgattgtaa
      541 aatcgaggaa ggatccatga ggtgtgatat aaatatctcc ctgaggcctg aaggctctga
      601 tgaatttggc accaaggttg aagtgaaaaa cctgggctct tttagagctg taaggcgtgc
      661 tattgagtac gaagaaagac gtcagaggga gatcctcgaa agcggtggaa aggtccttca
      721 ggaaacccgc agatgggatg aggctcgagg cattactatt cacatgagag ggaaggtgga
      781 aaaccgctac tcccttgagc caaatctagc tccggttatt atgaataagg agtggaaaga
      841 ggagattttt aggcagatac ctgaacttcc cgatgcaaaa aaggctcgct ttatcgagga
      901 atacgggttg cccgaatacg atgcctctgt cattactgct tcgaaaatcc tctcaaattt
      961 ctatgaggaa tgcgtaaaac attatcatga tccaaaaata ataagcaatt ggctcatggt
     1021 tgaattgctc agcatattaa atgaaaccgg acagggcatt aaggacttaa aatttactcc
     1081 caagcagtta tgtgatatgc ttgccatgat tgacaagggc actataagcg gtaaaattgc
     1141 caaagatgtc tttcgcgaga tgttcgacac cggcaagtcc cctgaggcaa tagtaaaaga
     1201 aaagggcctt attcagattt cggatgagtc ccagctagaa agcattgcaa aacaagtgat
     1261 agagaacaat cccaagtcag tagaagacta caagaacggc aagaaaaagg ccttaggttt
     1321 tcttgtgggt caggttatga aggaaaccaa gggtaaggcc aatccgcaga tggtaaatga
     1381 gatcttcatt cgcctgttaa atgatgaata aattgcaaac ccctacctgg aacaaatagg
     1441 gtaggggtta acttgtaaga atatcggtgg ataaatgtcc ctggcatgac cttataatcg
     1501 cgttgcctgt ggattgattt gtaaacagtg tcaatagcaa aattaaacga actatagtat
     1561 gatattaaat agtgaaatca aaatctataa aacggaggtt gacaatggca gtaattaaag
     1621 ctttcaaagc attgaggccg gtccctcatc tagtatcgaa agttgcagcc ctaccctatg
     1681 atgtggtgac tacaaaagaa gccgctgaaa tagcgaggga caacaggtac tcatttttgc
     1741 ggatagacag gcctgagatt gcttttgatg attccatgga cccctatgac acaaaggtat
     1801 acgaaaaagc tcgggaaata ttgttttcca tgatagacga gaaaatatac atacaggatg
     1861 ataaaccctg cctgtacata taccaattaa acagacaaaa ccgaattcaa aagggcttgg
     1921 tagcctgtgc atcaatcgat gattatttga atgatgttat aaaaaaacat gagcatactc
     1981 tgcctgtaaa agagcaggac aggatgaatc atgttaatta caccgaagcc catacgggcc
     2041 ctattctcat gagctatcgg gaaaacgtaa ggataacaga aattatagat aattggacat
     2101 catcccacaa tcccatttac gactttgttt caatggacca ggttggtcag accgtttgga
     2161 taattgatga ccccgatacc atagaggaaa tagtcaattt gtttgcaggt attgatgccc
     2221 tgtatattgc agatggccat catagggccg ctgcggccgt aaaggttggc cttgcaaaaa
     2281 agcgggaaaa tcccgattat acagggaaag aagagttcaa ctacttccta acggtaattt
     2341 tcccagatac gcaactgacc atattggctt acaacagggt tatcaaagat ttaaatggtc
     2401 taagcgaaca ggaattgctt gaaaaaatca aggagagttt tgatatagtt tacaagggcg
     2461 aagaagcctt catacccgat gcgaagcatt ccttcggcat gtatatagat aaaaaatggt
     2521 acggtttaaa agtcaaaccc ggcagcttta acgaaagtga ccccgtggcc agccttgatg
     2581 tttcgatact gcatgacctg ataattgcac ccgtttttgg aatcactaat cccagaaccg
     2641 atgaaagaat tgattttgtg ggcggagcaa aaggtgtgga agaattagaa aagcgggtga
     2701 acagcggtga aatgagcgta gctttttcac tgtttccgac acccatagaa gatctcatgg
     2761 ccatctcgga tgccggagct atcatgccac ccaagtcaac ctggtttgaa cccaaactgc
     2821 aaagcggtat atttatacac tccatgagcc tgtaagttac aagacaactt aatcaggaaa
     2881 taagactact cgtcaaaatg gaaagaggta ggaatatttt cctacctctt tttatggggc
     2941 atcttggtca ccgatgcctg gcaccagcga ccaagttgtt ttaagttagc ttaatctctc
     3001 cacgtcttaa aaaccgtttt ataatttttg atacataggg caccacatag taatcaaggc
     3061 cgaaggagcg accggcgcca gccagcatag caatagacac taccaggaac cacatatcac
     3121 cggaaccgga gatgataaag ttcagattca tgaatatcga cccgattgcc gccagcacgg
     3181 tgaacaggcc cagtataaag catatgccca gggcgatttc accgagtgtg atcatcacct
     3241 ggaagagata tgcattgggg tagatcacac tttcgacaaa ctgtgcgtac caatcaggag
     3301 tattaggccc tatcggtgcc gtagaagctc cggcgactaa cttatcacct gcagaaagcc
     3361 atccgctgtt taccttgtca atgcccgaca taagccattt gtagccgagg aagattctta
     3421 gaagcgccag ccatgctgcg cttgtgctca ctccaatgtg ttttatgaca gtcggcttat
     3481 ttctggtctt gcggaaaaac tggtggtcga tgtaatcgaa aataaaacca aagccgctaa
     3541 taccgaataa atagtgtata tctgccagat atttcgcagc agatgcgagg aagcctttga
     3601 gcttaaagcc catcacttca gcaatacaat actcggaacc gatggaaacc atatttccgt
     3661 gtagtttcag cttcaagggc ttcttggact tgttatttat ttcagctgct acgttgtatg
     3721 cagcacattt tgccgattga atcgcggatt ctaccaagca gggcattaca ttccctgatt
     3781 tatcttcata ataagtgtta tctccaatgg catatatgcc cggttcgctg ctttccataa
     3841 attcattgac gacaacttta ttgcgttttc cgagttcaag gccgctattt gcaactaagg
     3901 agcttgccct tacaccgccc gtccatatca gcgttctgct gtttacagtt tcaccggatt
     3961 caagtgtaag agagtttgct gttacattga ctatgcgggc atttgtaaga atttgaactc
     4021 cctttttcct gagatagttt gtggcatttt caatgaggct gtctgacatg gtgggcaaaa
     4081 ttgtcggcag tgcttctaca accattaatg atacttcact ggcctttata ttgtagtcga
     4141 tgcaaaggtc ttcaacccat tccaccaatt cgccgatcat ttcaataccg gtatagccgc
     4201 cgccacctac cacaaaagtc aaatattctt ttctcttttc cgcatcttct tcagtgcttg
     4261 ccaattcaaa catgtttact atgtgttctc taatcttaac ggcatcttca tgggaccaca
     4321 atgtgaaagc gttctctttc attcccggta tgtcaaaaaa tgcaggttcg ctaccgcatg
     4381 caagaatgag gtaatcaaag ctgtattcgt tgtctacacc gtaaagcctg cgattcttaa
     4441 catcgatttt attaatttca tcgaccacaa gtttgacccg ggtctggtcc aaggctgctt
     4501 ctagcgaata caatattccg tcatcgggaa tcctgtttcc ggcaatttca tgcagctgag
     4561 ttaaataaac ctggttggga gtcttattaa taaggatgat ttctacgttt ctattcttgc
     4621 gaagatatct atcaagggat tgagctgcag taagacctcc aaagccacct cccaatacga
     4681 caatctttac cttcttttcc atgttttatc ctcccctaaa attaatgctt gtttgcacca
     4741 tcacaatccg gtgcatagat tatcttacac gagagagcgc aatttaatac acatggtgtt
     4801 cataatattt tacaagatat tatacactat aatatattct atgtataaat ctaaattcct
     4861 gctcctttac tatacattaa accaaaaatt tggaacaagt caagtgattt atatcacatt
     4921 ttgtataaaa aattttacaa aagtacattt tttgcaggac tttttgtctt ttgtgcagaa
     4981 ataaagaatg aatattataa aagaataccg aaaaaagcaa atttttctgg aggaggaaaa
     5041 ggagggatca tcacgcggct aaacaaaaaa gttttcaggc ttataattgc agcttttata
     5101 atcgtcgcat gtatagctgc ttgcagtcgg aattccggag aaaagcctat tagtaagaca
     5161 aatttcatgc tggatacgct gatatccgtg caagcttatg gtcccaatgc atcgaaagcc
     5221 attgacaaag cctttgagcg aatccgcgag attgaaacaa agatgacatc aaaggcagat
     5281 tacagtgaag tgataggcat caaccagatg gctggtcaag acttttttaa agtgagtcct
     5341 gatacctatt atgtaataaa gaaaggtctt tattactcag agctttctga tggtaaattt
     5401 gacataacgg tagggccctt ggtggaactg tggggcatag gcaccgaaca tgctcgggtg
     5461 cctggaaatg atgaaataca ggaaatctta caatttgtag attatacaca agttgaaata
     5521 aaggatgaag acaatagcgt tttcttaaaa agtcctggta tggccataga tttaggtggg
     5581 atcgcaaaag gctatgctgc cgatgaagcg gcagatgtct taagacagaa tggcgttgag
     5641 catggcacgg tcaatttagg tgggaatatc gttgtaatag gggcaaaacc cgatggcaga
     5701 ccgtggaaaa taggcatcca aaaccctttt ttcaagaccc gagggtctgt tatgggctct
     5761 gtggaagtta cggataaaac tttagttact tcgggacctt acgagaggta ccttgagaaa
     5821 gatggcaaaa tataccacca tattttggac accaaatcag gttttccggt agaaaatgaa
     5881 ttgatgagcg tcacaatcat ttcggacagc tccatcgatg ccgatgcatt gtctacagct
     5941 gtttttgcaa tgggcttgga ggatggagtg gattttgtag agggccttga ttatttagat
     6001 gccgtcttca taacaaagga ttacaaggta tacattacgg cgggtgtagg ccgatataat
     6061 tttacgttga cggatgatca gtttgaatta atggagaggt aaaagcaaaa aatggtaaaa
     6121 gactgttaaa attttaacaa agtttttaga caatactaaa acattgtgac taatttcact
     6181 ttttctattg taaaaagaat attgttgtgc tagaattaaa attgcaaggg agtgatatgt
     6241 atctatgttc aagaaattta aaattccgga tgcgaccatt ggtaggttgt cattgtattc
     6301 caggtatctg gcggaagcag atgaaaaggg tataacaact gtatcttcgc agaatattgc
     6361 gcgagagaca ggtgtcactc ctgctcaagt ccgcaaggat ttagcttatt ttggagaatt
     6421 cggcaccaga ggtgtcggtt acaactcgag agagctttac aattacatca tgaaaatttt
     6481 aggtcttgat aagcgctggt cagttgccat tatcggtgcc ggaaacttag ggagggcgct
     6541 aacccagtac aagggtttcc agcagagggg ttttgacgta aagtgcatct ttgacaatga
     6601 tccgaaaaaa atcggaaaga agctggccaa tgtagagatt tatcctatcg attctctgga
     6661 agagaaagtt aaagaactgg atataaactt gggaataatt gccgttcctg caccttccgc
     6721 acaggaggta gctgataaac tggtcggtgc aggaataaaa gggattataa actttgcacc
     6781 tatcaatatt acagtagacg gaagcgtgat tttaagaaga gtagatttgg ctgctcagct
     6841 agaatacttg acttactatc tggaggaaac ttgatgaagc tgcttgaaat aaaagagctt
     6901 ctgcaggcca aagtcctgtg gggagaggag tttttagata tcgacgtatc caatgcctgc
     6961 ggagcagact tgatgagcga tattttggct gataacaaat ttaatgcaat tttgctcaca
     7021 ggcctaactc accggcagat cattcaaacc accgtcatgg gggattttgc agcaattgtt
     7081 tttgtgcgcg gcaaagttcc tcctgaggat gtcattgaac ttgcaaaagc agaaaagctt
     7141 cctttgctga caacgagttt tcccctttac gaatcctgcg gaatcctgta taaagcaggt
     7201 ttgggtcagg atagagttaa tggtgaggca tcagacaggt cttcaagggg agattccgaa
     7261 tcacccgaag ccatgcggct cacctatgac atcgtcggca atgactttaa cagtgccggc
     7321 aaagctgcag agcaggcaaa aagagtatta aaacaaatcg gcatagattc cggtgtcata
     7381 cgaaaggttt ccgttgctgc atatgaagct gaaatgaaca tcgtaataca tgcctataag
     7441 ggcaaattga tattcagcat tactccaaga tacatagaat tgatagcgga agatgaaggt
     7501 cctggaatcc ccgatataga aaaagccatg caggagggct attctacagc accagataga
     7561 atcagggagc tgggttttgg agccggcatg ggcttgccaa acatgaagaa attttcagat
     7621 ttttttgaga tagattcaga gcttggaaaa ggcacaaaag taagaatggt tataaaccta
     7681 tagcatcatc gaaagcggtg aaggacttgc agctttttta ccattcggtg actcttgatg
     7741 tagataagtg taaaggctgt accaattgca taaaagggtg tcctactgaa gcaataagag
     7801 tgagaaatgg gcgagcatat attctagaga acaagtgcat agactgcggt gaatgcatac
     7861 gcatatgccc caataatgcc aagtatgcag ctactgacag catcgataga ttaaaggatt
     7921 tcaagtttac agttgccctt cctgcaccaa gcctctttgg ccaatttaag gaaggcacta
     7981 caactaaaaa tgtcatatat gcacttaaga aaatcggttt tgacgaggtt tatcaagttc
     8041 ctctagcggc agaggaagta tccatagcta tacgtgaata catttccagg cacaagaatc
     8101 tacggccaat aatatcatca tcctgccctg ccgttataag actgatacag gtaagatttc
     8161 cagagctaat aaagaacatt atccccattc aatcgccgat ggaaatcgct gcaaagcgtg
     8221 ccaaagagat tttaggagaa aaatacgatc ttgctccaga ggagattggg gtatttttta
     8281 ttagcccttg ccctgccaaa gctacatcga ttaagcagcc catcggaacc ggtagatcct
     8341 atgtagatgg agtcatttcc atttccggta ttttcggcga aatagtcaaa aatctagatg
     8401 ctaaagcctt ccaatccgtt ttccccgatt cctcaggcat cggaattggc tggggcaggt
     8461 ccggagggga aaacatggct ataggcgggg ataattatct ggcagttgat ggaattcaaa
     8521 actgcataga agtgctggaa gaagtggaga tgaataaact ttctgatgtg gattatatcg
     8581 aatgccaagc atgtgtaggg ggatgcattg gcggtgcact taccgtggaa aaccactata
     8641 tagccagggt taaactgaga aggctatctg aaaagatggg ctatcagagc atattggatg
     8701 aagcatccgt aatagagcag ttcaaccaag gatattacca catggaggga gagatccaag
     8761 ccaagtctgc atttaagttg gatgataatt tagctgaagc aattcgaaaa atggagctct
     8821 tggagaaaac cgtcaaagat ttaccaggtc ttgattgtgg cagttgcggt tctcccaatt
     8881 gcagggccct ggccgaagat attgtcaaag ggctggcaaa tgaaactgac tgcatcttta
     8941 agttgaggga caaggtaaaa catttggctg ctgaaatgtt tgatttggcg caaaaaatgc
     9001 ctccaacaat ggaatctgag gataggggga atgatagcag gtgaaattga gcgaagttca
     9061 ggacttactt gggctaaaat tagttacaaa agtcacaaac cttgacagga aagttggagg
     9121 aggatatgct tcagacctat tgagctgggt catggcccat gccaaagaaa atgatatttg
     9181 gattaccatt caaagtcacc aaaatatagt agcagtggct tccttgctca gtttggcagc
     9241 gataattgtt gccgaagatg ccaacataga tgaaaacacg ataacaaagg ccgagcaaga
     9301 gaacatacca atctactcca gcgataagtc tatttatgaa ttatgcggta tactgtataa
     9361 tctgttgtca agggagtctg gataatgctt ttggaatacc cggcggattt acacattcac
     9421 acatgcctat ctccgtgtgc cagcgatgaa atgatacctg taaacatatt gaacatggca
     9481 gccctgatag ggactaaaat actaggcatc tgtgaccaca attctgcggg aaatatcgag
     9541 gccatgctga aagcggcacg agattatgat atcctggtaa ttcccggaat agaagtgcag
     9601 agtgccgaag aggtccacct agtatgcctc tttgaaacca taaatcagct tttacaattt
     9661 caagaattca tatacgatgc cttgccacca attaaaaaca atccgcagtt tttcgggcat
     9721 caatggctcg ttgacgatag aggaaattta ttaggtgaag aaactcgaat gctgttgacc
     9781 tcagcatcac ttacggtgga tgaagttgct caaaaggtgc atgaattaca agggttgata
     9841 attcctgccc atgtagatag aaggcacttt agcattatcg gccaattagg atttataccc
     9901 gaaaacttaa aaatagatgc agtggagttt tcaagggcta tttccctggc agaatttaag
     9961 tcaactttta aaagccttac gaacaatccg ttgattacat cttccgatgc gcacagtttg
    10021 caggaaatgg tttatcaaaa aacttatttc ctgttagaag aattaaattt taaagaaatt
    10081 attttagctt tgagaggttt ggaagggagg aaggtattga taaaggagta aaaggcactg
    10141 ttttataaca gatagtgaaa aagagaacaa taacatatga ggagaggata aaatggcagt
    10201 agctttaaat caacaacaag atttcatcga gaaactcaat aaaattgaag atatgttgaa
    10261 gaaataccag ggtaagagga gtgccctgat tcaggccctg caggaagcac aggctataat
    10321 gggctatctc ccaatagaag ttcaaaaact agtggcagaa aacttaggcg ttactttaag
    10381 tgaagtgtac agcactatta ctttttattc attttttaac ctaaagccca tagggaaata
    10441 tcatatcaga gtatgcttag gaacagcctg ctatgtgagg ggtgcagaaa aggtacttga
    10501 cagactaaaa actgaactcg gaattgaagt aggggacacc accgatgatt taaagttcac
    10561 cctcgatggc tgccgctgca taggcgcctg cggtctcgcc ccagcaatta tcgtcaatga
    10621 tgaagtgtat gggagactca ctccggataa aattccagag atactgaaaa aatttgaata
    10681 gccatgaagg aactttccct acacatttta gatatagtcc aaaactcatt aggggcgaaa
    10741 gcatcgctta ttgaaattaa tattaatgaa gatgtaaaaa atgacttatt ggtaattagg
    10801 ataaaggaca acgggctcgg catggatacc gccactgccc aaaaagtcat ggaccccttt
    10861 tatactaccc ggacaacccg cagggtagga ttgggcatac ccttattcgc ccaggccgct
    10921 gaaagctgcg gcggagattt taaaatatat tccgaaaagg gaagaggaac ggagatagta
    10981 gcaactttta cgcttagcca tatagatagg gcacctatag ggagtatggc agataccatg
    11041 gtatccttga ttgccatgcg gcctgatgtg gattatatat accgtcattg tgtaggggac
    11101 agagaattta ctttggatac ccgtgaaatc aaggaaaccc ttgaagatgt gccaattaac
    11161 aatccgatgg taattgactg gattaaagag tttgtgaatg gaaaccttaa ggaaattaac
    11221 ggaggtgcat aaaatgccag taataaagtc tatagaagat ctgaaaaaaa tccgtgatca
    11281 agcaaaggat cagataaatc tgcgagttga caatgaaagc aagacccgcg tagtcgtcgg
    11341 catgggtact tgcggcatag cggctggtgc aaggcaggta atgctggcca tactggagga
    11401 gctaaaaaag cgaaatgtct cagatgtcat agtaaccgaa gccggatgta tcggcctctg
    11461 ccagtacgaa cccttggtag atgtaataaa accggatcgc ccaaaagtta cctatgtaaa
    11521 tatgaatccc gataaagcca gggagatagt ggtaaaacac attatcaacg atcaagttgt
    11581 ggatgaatat gtagtaccta atatttaatg gataaataca ggagcttgag gaggttatga
    11641 aaatggaaat atatagggct cacgttttgg tttgcaaagg aacaggttgt gtagcttccg
    11701 gcagtgaacc aatctttgaa acactacaga aggaaatcga gaaaaaaggc cttgataaag
    11761 aggtaaaggt tattcaaacc ggttgcttag gcctttgtga actaggaccc aatattttga
    11821 tttaccctga aggcagttat tactgtcagg ttaaggcaga ggatgtaccg gaaattgttg
    11881 aggaacatct gttaaaagga cgcgtagtcc aacgccttct ttataaagag ccggattcta
    11941 aggaacgcta tcgctcttta atggatatag acttttacaa gcgtcaaaaa cgcatcgcct
    12001 tgaggaactg cggtattatc aatcctgaaa taattgaaga atacattgca aatgacggtt
    12061 atgcagcctt ggcaaaagca ttgactgata tgaaacccca ggaagtcatt gatgaggtaa
    12121 agaaatccgg cctaagaggc cgtggaggcg gaggattccc cacaggtatc aagtggcagt
    12181 ttgctgcaaa tgcagaaggt gatatcaaat acgtagtttg caatgcggac gaaggtgacc
    12241 cgggtgcttt catggataga agtattctgg aaggcgaccc ccactcggta ctggaagcca
    12301 tggcaatagc tggttatgcc attggtgcca gtgaaggata tatttatgta agggcggagt
    12361 acccgatagc cgtaaaacgc ct
//
LOCUS       DAONUO010000011        11664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4251_length_11664_cov_3.466448, whole genome shotgun sequence.
ACCESSION   DAONUO010000011 DAONUO010000000
VERSION     DAONUO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11664
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4251_length_11664_cov_3.466448"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PK768_00860"
     CDS             complement(<1..266)
                     /locus_tag="PK768_00860"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HQA59552.1"
                     /translation="MQKILGEVLEENRELAKKGRVASYIPALAKANPDHLGICIAEID
                     GNTYKAGDCDVKFTIQSISKVIALMLALMDNGEKVFEKVGREGT"
     gene            496..1038
                     /locus_tag="PK768_00865"
     CDS             496..1038
                     /locus_tag="PK768_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQA59553.1"
                     /translation="MKELWKLFVTFSRIGALTFGGGYAMLPMIQREIVEKNRWATEEE
                     VMDYYAIGQCTPGIIAVNTATFIGFRQRGIPGAIAATMGMVFPSLIIISIIAMSFKEF
                     QDIAIVRHAFGGIRVAVAALIANTVLKLLRGSVKDRLGIFIASLSFISVALFKLSPIF
                     AVVASAALGIISKTVRKSEN"
     gene            1035..1598
                     /locus_tag="PK768_00870"
     CDS             1035..1598
                     /locus_tag="PK768_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQA59554.1"
                     /translation="MIYLQLFFEFFKTGLFAIGGGLATLPFLQNISEKYGWFSSRELV
                     DMIAVSESTPGPIGINMATYAGYKTANLPGAIIAVIGEVAPSIIIIVLIAHYYMKFNE
                     HPKVAAAFYGVRPAVAGLIGAAGFEVAKVALFNINKYLSTKNVLDIFDVKSLLLFAFI
                     LISSNKYKKHPVLFLLAAAAIGIIFKY"
     gene            1904..2239
                     /locus_tag="PK768_00875"
     CDS             1904..2239
                     /locus_tag="PK768_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HQA59555.1"
                     /translation="MERFTLNSEDAVLMVVDIQEPLVPVMKYGKQVIENCNIIITIAK
                     DFGIPIIVTEQYPKGLGPTVPELRSNLERSVTYEKISFTAFTDEVAAELEKLGRKKVI
                     VEKRPILYA"
     gene            2411..3586
                     /locus_tag="PK768_00880"
     CDS             2411..3586
                     /locus_tag="PK768_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HQA59556.1"
                     /translation="MSKLLKLSKPSLKKTGMLLVLLLLIMTLSSSIAVAAGPLGVSTQ
                     YPGISAQPGSSVSFPLTISGTGFVDLEVVSIPSGWTAIIFGAGKKIHQVYVPSDDVAA
                     AELRVQIPSDAKNGSYDIVIEASGSGGRARLPLNIIIDTDASGADKIEVQYPALSGPD
                     TATFSFRGTLYNNSGRERFYSLGAAAPDGWQVAFKPAYQSNQITSLSLEAGKSQDLDI
                     SVKPPSGVKAGTYTITAAAASGSEITKVDLTVEIAGTYRMELSTPSGRLNLNTTAGKQ
                     GALTLELENTGSADLLGINLSASTPPGWAVTFEPEKVERIKAGEKQQITAFITPDSKA
                     IAGDYIVNIRASAPETSDSADIRVTVQTSTLWGIVGVAIIAGVIYWVVTTFQKYGRR"
     gene            3589..4542
                     /locus_tag="PK768_00885"
     CDS             3589..4542
                     /locus_tag="PK768_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA59557.1"
                     /translation="MNDGIIIETQGLTKVYGSITAVDNLNLTVRKGEIFGLLGPNGAG
                     KTTTILMLMGLSEPTSGSIRVAGLDPVRQALGVKHIVGYMPDNIGFYGDLTGRENLFY
                     TAQLNGIAPDVAKSKIDELLTKVGLQEAADRRVREYSKGMRQRLGIADVLIKDPEIIF
                     LDEPTLGLDPEGTKELLNLILELGKREGKTVLISSHLLHQVQAICDRVGIFVKGKLVA
                     IGSVSDLAKEIGSEVIVELKTSNMEDFPTHEVNAMPGISSVQMEKDMIVIKSDDPEIA
                     PKLVRELNRMNIDIYHVRSRSSDLDDIYSRYFHEGEAKLNV"
     gene            4535..5554
                     /locus_tag="PK768_00890"
     CDS             4535..5554
                     /locus_tag="PK768_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQA59558.1"
                     /translation="MSKLNKLKEKFQKQLAAFKADSNSQGSSNNLATVFFKELSDQLS
                     STRFFILFSLITLTGIVSFYSAASNIRGTTDDAVASFVLIRLFTTSGSSLPSFTWFLS
                     ILGPLVGLSMGFDAINAEQNSGTMTRLLAQPIYRDDVINGKFLARLFVLVLITLGLGF
                     FVAGMGIIITGVPPSGEEVLRLLLYLGLSIVYMGFWLALAMLFSLVFRHTATSALCCI
                     SLWLFFSIFVPILAGMVADAIFPITQSSDTMTQLYNVRVSQALSRLSPSTLYSEASVT
                     LLSPTVRTLGPVLLEQTFGAIAGFLPLGQSLLLIWPHITGLVAGTLFIFGIAYTIFLR
                     QEIRA"
     gene            complement(5747..6523)
                     /locus_tag="PK768_00895"
     CDS             complement(5747..6523)
                     /locus_tag="PK768_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQA59559.1"
                     /translation="MATRVSVSFEDKVAYLTFSEENERRPCTLDWDVLKELRNAVSQI
                     EDNIPDTRLVIVQSASPKSFIVGANIAALEELNPENIMEWVRQGHDVFLRLRKLPIPV
                     IAKVESYALGGGLELAMACDFIVATENAVFAQPEASLGVMPGWGGSFRLINLVGPNRA
                     KQLFMTGEHISAKQAYEWGLVNLVCSAEEIDEKLNEIAEKIVQNDYQVLAFVKRMINN
                     ISDVRDENGILLEEISSSVCLNSESTKARLNAFFEKRRKK"
     gene            complement(6560..8080)
                     /locus_tag="PK768_00900"
     CDS             complement(6560..8080)
                     /locus_tag="PK768_00900"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HQA59560.1"
                     /translation="MAKIVSVEEALAQIKDSDTVAIQGSGGGVCEPTFILKKLGEKYC
                     REKSPKNLTLLHANGIGDRDTYGTDALAYPGLVKRDIAGHWGMAPKMAQLALEEKIEA
                     YNLPQGVISQMFQTIASGKPGVITKTGLHTFIDPRIEGGKMNKSAKEDLVKVIEIDGE
                     EWLYYPNIKIDVAIVRGTTADTKGNISSEEEAAILEGISIAQAAHNCGGIVIAQVKYI
                     AEHGTINPKDVTIPGIYVDYVVQDPDQQITCDRVYDPMLAGNVRAPFERIPPLPLSSR
                     KVIARRAAKELKKGDIINLGVGIPAGIAAVAAEDGYISDLNFTIEQGLIGGMPMGGVI
                     FGVSYCPEAVVDQSLQFNFYDGGGLDVCFLGFAQIDKEGNVNSSKTGNLLSGCGGAIN
                     ISQNAKKVVFCGTFTAKGLEQDISNNEIKIVKEGSIKKLVNKVDQITFSGNYAKSINQ
                     EVYYITERCVFKLANEGLCLTEIAPGIDLQRDILEQMEFEPIIAENLKVMDRNFFE"
     gene            complement(8105..8518)
                     /locus_tag="PK768_00905"
     CDS             complement(8105..8518)
                     /locus_tag="PK768_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQA59561.1"
                     /translation="MVDIPFEIGQSVTFSKTVSESDVYLFAGITGDFSPNHIDEEYMK
                     QTPYKKRIAHGVLSIALSSTASTKFCQMSKSPCVSYGYDRIRFIKPIFIGDTVTVKYT
                     CTEIDRENHKTYAKIEITNQHGELCTVGTHILKFL"
     gene            complement(8565..9953)
                     /locus_tag="PK768_00910"
     CDS             complement(8565..9953)
                     /locus_tag="PK768_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59562.1"
                     /translation="MSKKKLAFVTLGDTRLEFYKKREHIVKEESQKLLDILKDRYELY
                     VPETVFNIEEGQKVADEIRSRGISAVIIHLPIWATPNLAFRVAYSTDKPVLLVGNKRQ
                     DSSSLVTMLAIAGMLDQTGKECYRLLVDVNNAKDLDEIDVFVSACYLVDELARSSYCL
                     LGGRSIGIGTTVADPSQWQSLFGVEFDHKDQYEIVYRSKTYSDEKVAFYKDWINKAFK
                     VDFGDLFSEETLDLQIRSYLALKDIVKENHYNFLGIKCQTDMSDHFVLQCLPIALLNN
                     NYDAEGPKEVIPTACEADCDGALTMKLLSIASGNNPSNLVDIRHFDADKKELILANCG
                     SVAPFFSKPSSNGDDYSTTLMPHVFGKAGGGSLQMIAKEGKVTLARLFRSKGKYVMGC
                     FEAELESRPIEELKKTTWCFPHEFVKADIDYDKFFRTMNSNHLHTVYGSYGKVLELFC
                     KMKGIEFINYNA"
     gene            complement(9987..11279)
                     /locus_tag="PK768_00915"
     CDS             complement(9987..11279)
                     /locus_tag="PK768_00915"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HQA59563.1"
                     /translation="MMILITLVMTFIVLLIIGVPIAYVLGCTAMLGIWLLPGMSLITT
                     VQRMFSGLNSFTLLAVPLFIFAANVMNQSGISERIVDFAVAIVGRVRGGLAYANVLDS
                     MLFAGLSGSAQADTAGLGKVVIPAMIREGYDKETAVGVTAATSTLATIIPPSVTMVVY
                     SSVAGVSVGAMFLSGIVPGILIGLGMMGVVYYHAVKHDYPRHTGTTLKQLLRLTVVNV
                     PALLTPLIIIGGINLGFMTATEAASVACIYAMLLGFFAYRSLKLKDFKALLEDTVRTS
                     AVSTFALATANALGQLLSYYNFNQHVGEFFSATFDSRFSFLAIVVLFYIFIGTFMDAI
                     PAIILFVPALMPTATSLGISGIQLGLVIIITLAIGLITPPYGLCLLIASKIGDLTVDR
                     SFYAAAPYIAVALFVVFLVVFVPDVALGLPKLIRPELF"
     gene            complement(11295..>11664)
                     /locus_tag="PK768_00920"
     CDS             complement(11295..>11664)
                     /locus_tag="PK768_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HQA59564.1"
                     /translation="EIANYAFIWLAFVGSSICIRSDEHFRFTAFTERLSEKGKLINKL
                     VVQLILLVFNCIVAYFGVMLFLKFRTWKFSSLPSVSLGWAWLCIPIYGFTAALYNLEQ
                     IYSLLKFGVPTKGEISQDSA"
BASE COUNT         3160 a         2683 c         2464 g         3357 t
ORIGIN      
        1 tccgtgcctt ccctgcccac cttttcaaag accttttccc cgttgtccat gagggctagc
       61 attagagcga ttacttttga tatgctctgg atcgtaaatt taacatcaca atctcctgct
      121 ttataggtat tcccgtctat ttctgcaatg catataccta ggtggtcggg atttgctttc
      181 gccagggcag gtatgtaact ggcaacacga ccctttttag caagctcccg gttttcttca
      241 aggacttccc ctaaaatctt ttgcatagaa cccaccttcc attttaaagc atcgtttcac
      301 ttgttttttt gtaatctttt gaatagtgtc tttgcattta tataatacca cttttctgat
      361 tacaaggcaa gccattttcc taaatgctgt tatatctttc ataaacttcc aatgctctgg
      421 tacgctttat ttttttgctg caaagtgtta aactcattga tgtacagata atttataaac
      481 attaaggtgg attttatgaa agaactatgg aagctttttg ttacttttag ccgcatagga
      541 gccttgacct tcggcggggg ttatgccatg ctgcccatga ttcaaaggga aattgtagag
      601 aaaaaccggt gggcaactga agaagaggtc atggattatt atgccatcgg ccagtgcaca
      661 ccgggaataa tagccgtaaa tactgcaacc tttataggtt ttaggcaaag gggcatcccc
      721 ggtgccatag ctgccaccat gggcatggtg tttccttccc tgataataat cagtatcata
      781 gcaatgtctt ttaaggaatt ccaggatatc gccatcgtca gacacgcctt tggaggtatc
      841 agggttgcgg tggccgcact tattgcaaat acagtattaa agcttttgag gggctccgtc
      901 aaggatcggc taggaatctt tatagctagt ctgtcattca tctccgttgc cctctttaag
      961 ctttccccga tttttgcagt tgtagcatcg gctgctttag gaataatttc taaaacagta
     1021 aggaagagtg aaaattgatt tatctacagt tattttttga attctttaag acgggcctat
     1081 ttgccattgg tggaggtctt gccacacttc catttctcca aaatatatca gaaaaatacg
     1141 gctggttttc atcccgggag ttagtcgata tgattgccgt atccgaatcc actccaggcc
     1201 ctataggaat caatatggca acttatgcag gatacaagac tgccaatttg cctggggcca
     1261 taattgcggt gattggggaa gttgcccctt ctataattat tattgtatta atcgcccact
     1321 actacatgaa attcaacgaa cacccgaaag ttgcggccgc attctacggg gttagacctg
     1381 ccgtggcagg tcttatcggc gctgccggtt ttgaggttgc aaaagttgct ctgtttaata
     1441 ttaataaata cttatctact aaaaatgtat tagatatttt cgatgtaaaa tcactgttgc
     1501 tcttcgcctt catcctcatc tcatcaaata agtataaaaa gcatcccgta ctttttcttt
     1561 tagcagctgc cgcaataggt ataatattta aatactgatt gcagtttgac ctggttatac
     1621 ccaacaattc gcccccctat attcaatatg aggtcatttc aaagggcaaa ttgttttatg
     1681 aaacagataa aaagtttcga tgtgaatttc aagctatcgc tttacatagg tactttgata
     1741 ttaagccact gtatgatgaa tacaactttt atcttaagaa acgaatctta gaaggaagga
     1801 tgctcaatgg ataacgaaaa aatccttcaa aatcaattaa ctgactttga agaattcgca
     1861 aaaataattg taagggaaat atcataatgg gggaggtctt cacttggaaa gatttacgtt
     1921 aaactctgaa gatgccgtct tgatggttgt cgatattcag gaacctttgg tgcctgtaat
     1981 gaaatacggc aagcaggtta ttgaaaactg caatattata atcactatag ccaaagactt
     2041 tggaattcct ataatagtta cggaacagta tcctaagggc ttggggccaa cggtcccgga
     2101 attgaggagc aatcttgaaa gaagtgtgac ttacgaaaaa ataagcttta cagccttcac
     2161 tgacgaagta gccgctgagc tagaaaaatt ggggaggaaa aaggtaattg tagaaaagcg
     2221 gcctattctc tatgcctaat tcatcctctc tattgttata atttatcaaa aatacatatg
     2281 cttgctctga tttttaaagc ttcaatcgct accaaaaaac tataacaatt tcctcacatt
     2341 tttttcaaaa ttatatttta aaatgtctta gtaggctaat cataacactt tgagggaagg
     2401 tgatgaagtt ttgtcgaaat tattaaaatt gagcaagccc agcttaaaaa agaccggaat
     2461 gttgctagtg ctattattgc ttattatgac cctctcatcc agtatcgctg ttgctgccgg
     2521 tcccctgggt gtttcaactc aatatccggg catttcggcc caaccgggaa gcagtgttag
     2581 ctttcctctc actattagtg gaaccggatt tgtagacctt gaggttgttt caattccttc
     2641 tggatggact gcaatcattt ttggcgccgg caaaaagatt catcaggttt acgtcccctc
     2701 agatgatgtg gcagctgctg aactacgggt gcagatcccc tcagatgcaa aaaacggcag
     2761 ttatgatata gtgattgaag catctggcag cggtggccgc gcaagattgc ccttaaatat
     2821 tataattgac accgatgcca gcggtgcaga caaaattgag gtccagtatc ctgccctttc
     2881 cggccctgat acggcaacct tcagttttag gggaacccta tacaacaaca gtggtcgtga
     2941 gcgcttttac agcctcggtg ctgctgcacc cgatggttgg caggttgcct ttaaaccggc
     3001 ttatcagagt aaccagataa cgagcctcag cttggaagcc ggcaagtccc aggatttaga
     3061 tatatcggtt aaaccaccta gcggtgtcaa agctggaaca tacacgatca cggcggcagc
     3121 tgcctccggt agtgaaataa ctaaagttga cctgacggtt gaaattgctg gcacctatcg
     3181 catggaactt tcaaccccct cggggcgctt aaacctgaac accactgcag ggaaacaggg
     3241 agccctaacc cttgaactgg aaaacacggg tagtgcagat ttgttaggca taaatctttc
     3301 cgcttcaact ccacccggat gggctgtgac ttttgagccc gaaaaggttg agcgcataaa
     3361 ggccggtgaa aagcagcaga ttacggcttt tattaccccc gacagcaagg caattgccgg
     3421 cgactacata gtgaacatca gggcttcagc ccctgagacc tcggatagcg ccgatatccg
     3481 tgtaaccgtc caaacctcca cactatgggg catagtagga gtagccatta tagcaggtgt
     3541 tatatattgg gtagtaacaa cctttcaaaa gtatgggcgg cgataaccat gaatgatgga
     3601 ataattatcg aaactcaagg gttgacaaag gtttacgggt cgataaccgc cgtagacaac
     3661 ttaaatctta cagtgagaaa aggcgagata ttcggccttt taggtccaaa cggtgcgggt
     3721 aaaaccacta ccattttgat gctgatgggc ctttccgagc ctacttcagg cagcattcgg
     3781 gtagcgggac tcgaccctgt gcgccaagct cttggagtta aacacatagt gggatacatg
     3841 cccgacaaca taggcttcta cggcgatttg acaggcaggg aaaatctctt ttacactgcc
     3901 caattaaacg gtatagctcc tgatgttgcc aagagtaaaa tagacgagct tctgactaaa
     3961 gttggtcttc aggaggctgc cgacaggagg gttcgtgagt actccaaggg aatgcgtcag
     4021 cgacttggca ttgccgatgt tcttataaaa gatccggaga tcattttttt agacgaaccc
     4081 accctgggcc ttgacccgga gggaactaaa gaattgctga atttaattct agaacttggc
     4141 aaaagagagg gcaagaccgt tctcatctca tcccatcttc ttcatcaagt ccaggctatt
     4201 tgtgataggg ttggaatttt cgtaaagggc aagttggtcg ccatcggcag tgtctccgat
     4261 ttggccaaag aaatagggag cgaagtaata gtagagctga aaacaagcaa tatggaagat
     4321 ttcccaactc acgaagtaaa tgccatgccc ggcataagtt ctgtgcaaat ggaaaaagat
     4381 atgatagtca ttaaaagtga tgatccggaa attgccccaa aactcgtaag agagcttaat
     4441 agaatgaaca tcgatattta ccacgtaaga agtcgcagct cggacttgga cgacatttac
     4501 agccgctatt tccacgaagg ggaggcgaag ctgaatgtct aaactaaata agcttaaaga
     4561 aaagtttcaa aaacaactag cagcttttaa ggcagactca aacagccagg gcagcagtaa
     4621 caatttggca acggtgttct tcaaagagct gtccgaccag ctttccagca ccaggttttt
     4681 tatccttttt tccttaatca ctcttacagg tatcgtttca ttttactctg ctgcctccaa
     4741 cataagaggt acaaccgatg atgccgtggc aagttttgtt ttaatcaggc tctttacaac
     4801 atccggctct tccctcccgt cctttacctg gtttctttcc atactggggc cattagtggg
     4861 gctttccatg ggcttcgatg ccataaacgc cgagcaaaac agcggaacga tgacaaggct
     4921 cctggcccag cctatatata gggacgatgt aattaacggt aagtttcttg cgaggctttt
     4981 cgtgcttgta ttgataactt tgggtttagg cttttttgta gccggcatgg gcattataat
     5041 cacaggagtc ccccctagcg gtgaagaagt gctaagactg ctcttgtatc tcggacttag
     5101 catagtgtac atgggatttt ggctggcctt ggccatgctc ttttcccttg tctttcgcca
     5161 cacagccaca tctgcactct gctgtatctc gttatggctg tttttctcaa tctttgtccc
     5221 catactggca ggtatggtag cagatgccat tttccctata acccaaagtt ccgatactat
     5281 gacccagcta tacaatgtca gggtctcaca ggctttaagc aggctgtctc cttcaacact
     5341 ttacagcgag gcttcggtaa ccctgctttc acctaccgtt agaactttag gcccggtgct
     5401 gttggaacaa acctttggcg caattgccgg ttttctgccc ttgggccaaa gcctactttt
     5461 gatatggcct cacattactg gccttgtggc gggaacactt tttatctttg gaatagctta
     5521 taccatattt ttgcgccagg aaatcagggc ataagaatac tgccgcgccc tatcatacta
     5581 gcaggctcga acaaaatagg gacttcgcgc ttataaggct gtagattagg ataccaaaca
     5641 gaaagaaaat cggcttagga catttatccc taagccgatt attgttaaca ccggtgaaaa
     5701 ccagcttttg ttttcaaagc aaagctgaaa tacttatcat cctgtcttat tttttccgcc
     5761 gcttttcaaa aaaggcgttc aggcgtgcct ttgtcgactc tgagtttagg cagacgctgg
     5821 aggatatttc ttcaaggagg atgccgtttt catccctgac atccgatata ttgtttatca
     5881 tccgcttgac aaaagccagg acctgataat cattttgtac tattttttca gctatttcat
     5941 ttagcttttc atcgatctct tcagcagaac atacgagatt gactaggccc cattcatagg
     6001 cctgcttggc gctgatatgt tcgccggtca tgaaaagctg ctttgcccta ttgggaccaa
     6061 ctagatttat cagtctaaag gagccgcccc agcccggcat tactcccaag ctggcttccg
     6121 gttgggcaaa tacggcattc tcagttgcga cgataaaatc acaggccata gccagttcca
     6181 ggccgccgcc caaagcgtaa ctctcaacct tagcaattac cggtatgggg agtttcctca
     6241 gtctgaggaa cacatcatgg ccctgccgca cccattccat gatgttttcg ggattgagct
     6301 cttccagcgc ggcaatattt gctccgacta taaaggattt gggcgatgcg ctctgaacta
     6361 ttacaagcct ggtatcgggt atattatctt cgatttgtga aacggcattt ctcagttcct
     6421 ttaatacgtc ccaatccagg gtgcagggcc gcctctcatt ctcctcactg aaagtcaaat
     6481 aagccacttt gtcttcaaaa ctgacactaa ctcttgttgc caaattaatc acgctccact
     6541 gatattaagc aagatgcttt tactcaaaaa aattcctatc catcactttt aggttttcag
     6601 cgataattgg ctcaaattcc atctgttcaa gaatgtccct ttgtaaatct atgccgggtg
     6661 ctatctccgt caggcacaag ccttcattag ctagcttaaa tacgcacctt tcggtaatgt
     6721 agtagacctc ctgatttatg ctctttgcgt aattgccgct gaaggttatc tggtctacct
     6781 tatttacaag ctttttaatg ctgccttctt ttacaatctt gatttcatta ttgctgatat
     6841 cttgctctaa gccctttgcc gtaaatgtac cgcagaagac caccttttta gcattttgcg
     6901 atatgttgat tgctcctccg caacctgaca ggagattccc ggttttgctg gaattcacat
     6961 taccttcctt gtcaatttgg gcaaagccta agaagcagac atcaaggcca ccgccgtcat
     7021 agaaattgaa ttgcagggat tggtccacaa cggcttccgg gcaataagac acaccaaaga
     7081 tgactcctcc cataggcatt ccgccaataa ggccttgctc tattgtaaag ttcaaatcag
     7141 atatataccc gtcctcagct gcaacggctg cgattccagc cggtatgcca acacctaaat
     7201 ttatgatatc gccttttttt aattccttgg cggcacgccg ggcgataacc tttcgggagg
     7261 aaaggggcaa tggaggtatc cgctcaaagg gagcacgcac gttcccagca agcatggggt
     7321 catacaccct gtcacaggtt atctgctgat ctgggtcctg aacgacatag tcaacgtata
     7381 tgccgggtat cgtaacgtct ttgggattga tagtgccgtg ttcagcaatg tactttacct
     7441 gggctattac aattccaccg caattgtggg cagcttgggc gattgatatt ccttcaagta
     7501 ttgcagcctc ttcttcggaa ctgatattcc cttttgtatc agcggtggta cctctcacta
     7561 tagctacatc gattttgatg ttgggatagt aaagccattc ttctccgtca atttcaatga
     7621 ccttcaccaa gtcttcctta gccgatttat tcattttgcc accttcaatg cgcgggtcta
     7681 tgaaggtgtg caaaccggtt tttgtaatga ctcccggttt tccagaagca atcgtctgaa
     7741 acatctgtga aataacaccc tggggcaggt tgtaggcttc gattttttct tccaaggcta
     7801 gctgtgccat cttgggagcc attccccaat gccctgcaat atcccttttt acaagacccg
     7861 gatatgcaag ggcatcggta ccgtaagtat ctctatcgcc tataccgttg gcatgcagca
     7921 acgtcaagtt tttaggcgat ttttccctgc agtacttctc acctaatttt ttcaatataa
     7981 aagttggttc acaaacgcct ccgccggagc cctgaatggc aacggtatcg ctatccttaa
     8041 tttgcgccaa ggcttcttcc acacttacga ttttagccaa taaatgccac ctccattttc
     8101 aaatttacaa gaacttgaga atatgcgttc ccactgtgca cagctctcca tgctgattgg
     8161 ttatttcgat tttggcatag gttttgtggt tttccctgtc gatttcggtg caggtgtact
     8221 ttacagtcac cgtatccccg ataaatatgg gcttgataaa tctaatcctg tcatagccgt
     8281 aggagacaca gggtgattta gacatctggc aaaacttagt agacgctgtg gatgaaaggg
     8341 caattgagag cacgccatgg gctattcgct ttttgtaggg tgtctgcttc atgtattctt
     8401 cgtcaatgtg attgggtgaa aagtcgccgg taattcccgc aaacaggtac acatccgatt
     8461 cactgactgt tttactaaat gtaacgcttt ggcctatctc aaagggaata tcaaccatca
     8521 aagccacctc caatctaaaa ttgtaaaggt tcatatgcct gcgattaggc attgtaattg
     8581 ataaactcta tacccttcat tttgcagaac agctctaaca ccttgccgta gctgccgtat
     8641 actgtatgca aatgattgga gttcattgtg cggaagaatt tatcgtaatc aatatcggct
     8701 ttcacaaact cgtggggaaa acaccaggtg gtttttttca gttcttctat gggccttgat
     8761 tccagttcgg cttcaaagca gcccatcaca tatttgccct tgctcctaaa aagcctggct
     8821 aaggttactt tcccttcttt ggcaatcatt tgcagtgacc cgccccctgc ttttccaaag
     8881 acgtgaggca tcagtgttgt agagtaatca tctccattac ttgagggctt tgagaaaaat
     8941 ggtgcaacgc tgccgcaatt ggccaagatc aattcttttt tatctgcatc aaaatgcctt
     9001 atgtcaacca agttacttgg attgtttccg ctggcaattg ataacagctt catggtcaat
     9061 gcgccatcac aatcggcttc acaagctgta ggtataactt ccttaggacc ttcagcgtca
     9121 tagttattgt tcaaaagtgc tattggcagg cactggagca cgaagtggtc actcatatcg
     9181 gtctgacact ttatgcccag gaagttgtaa tgattttctt tgacaatgtc ctttaaggcc
     9241 aaatagctcc tgatctgaag atctaatgtt tcttcgctaa agaggtcacc gaagtctacc
     9301 ttaaaggcct tgttaatcca atctttgtag aacgccactt tttcatcgga ataagttttt
     9361 gacctataca cgatttcgta ttggtctttg tggtcaaatt caacgccgaa caggctctgc
     9421 cactgagagg ggtcggcaac ggtagtgcct attcctatgc ttctgccgcc caacaggcag
     9481 taactgcttc tcgcaagctc atccaccagg tagcaagcgc tgacaaaaac atcgatttca
     9541 tccaaatctt ttgcattatt gacatcaacc aagagcctgt agcactcttt tccggtctgg
     9601 tccaacatgc ctgcaatagc cagcattgtt accaaacttg agctgtcctg gcgcttgttg
     9661 cctacaagca gtacgggttt atccgtgctg taagccactc taaaggccag gttgggtgtt
     9721 gcccaaatgg gcagatgaat gatgaccgct gagatacccc tgctcctgat ttcatcagct
     9781 actttttgtc cctcttcgat gttaaaaacc gtttccggca cgtaaagttc gtatctgtct
     9841 tttaatatgt caagtaattt ttgggattct tctttaacta tgtgttcacg ctttttgtaa
     9901 aactcgagcc tggtatcacc tagggtgaca aaggccagct tttttttgct cattcgacat
     9961 cacctttcat ttaaacaaac aattaattaa aacaattcgg gtcttatcaa cttaggtaat
    10021 cccaatgcaa catccggaac aaatacaacc aagaatacca caaatagcgc tacggctata
    10081 taaggtgccg ccgcatagaa ggatctatcc accgtcaggt ctccaatctt gctggcgatt
    10141 agcaggcaca gcccgtatgg aggagtgatt aagccaatgg caagggtaat gatgattact
    10201 agacccaact gaattccact tatgccaaga gaagttgctg ttggcatgag tgccggcaca
    10261 aagagaatga ttgcgggaat agcgtccata aatgtcccta tgaagatgta aaatagtacc
    10321 acaattgcca ggaagctaaa tctactgtca aaagtagcgc tgaaaaactc tcccacgtgc
    10381 tggttaaagt tatagtagct taaaagctgt cctaaggcat ttgcagtagc caacgcaaag
    10441 gtggaaactg cactggtcct caccgtatct tccaaaaggg ccttaaaatc ctttaatttt
    10501 aaagatctat atgcgaaaaa gcccagtagc attgcataga tgcaagcaac acttgctgct
    10561 tcagtggcag tcatgaaacc aagatttata ccacctatga taattaaggg agtaaggagt
    10621 gcaggtacat taacgactgt aagcctcaaa agctgtttta aggttgtgcc ggtatgtcta
    10681 ggataatcgt gctttacggc gtggtaatat actaccccca tcattcctaa gcctatgaga
    10741 atgcctggga ctattccgga aagaaacatg gctcctactg atactcctgc tacgctgctg
    10801 taaactacca tggtcacgct aggaggtata atggttgcca gggtagaagt tgcagctgtc
    10861 actcctacgg ccgtctcctt gtcatagcct tctcttatca ttgcaggtat gactacctta
    10921 cctagacctg ccgtatccgc ctgagctgag ccggaaagtc ctgcaaaaag catggagtct
    10981 aacacgttag catatgctaa gccgcctctt actctgccca caatggcaac ggcaaaatct
    11041 actattcttt cagaaatacc ggattgattc atgacattgg ctgcaaatat gaacaaagga
    11101 accgccagta gcgtaaatga atttaaaccg ctgaacatcc tttgaactgt agtaatcagg
    11161 gacatccccg gcaagagcca aatgccaagc atggccgtgc agcctaaaac gtaggcaatt
    11221 ggcacgccta ttatcaagag cactataaag gtcataacta aggtaattag tatcatcata
    11281 aatctcacac cctcctatgc tgaatcctgg cttatttcac cttttgtggg cacaccaaat
    11341 ttcagcaagc tgtatatttg ctctagatta tataaagctg cagtaaaacc gtatatggga
    11401 atacaaagcc aagcccagcc caaactgaca gatggtaaag aactgaattt ccaggtccta
    11461 aactttaaaa agagcattac gccaaaatat gctactatgc aattaaagac aagcaatatt
    11521 agctgtacta ccaatttatt tattaatttg cctttctccg acaacctctc ggtgaaagcg
    11581 gtaaatcgga aatgttcatc tgatcttata cagatggatg aacccacaaa tgccagccaa
    11641 ataaaagcgt agttggcaat ctct
//
LOCUS       DAONUO010000012        11422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4379_length_11422_cov_3.448931, whole genome shotgun sequence.
ACCESSION   DAONUO010000012 DAONUO010000000
VERSION     DAONUO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4379_length_11422_cov_3.448931"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PK768_00925"
     CDS             complement(<1..1287)
                     /locus_tag="PK768_00925"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HQA59565.1"
                     /translation="MDFFNVTILPDSVQIKVPADFTLKQVFNENGINFEFPCGGLGVC
                     KRCKVTIIRSDGQVQEALACQYRVKEDVTVKLPHKEAKHAILSEGIERHVAVDTLVKK
                     VYFELPHPTLSDNRDDWKRVIEQQGIKTSLKVLQDLPEKLRESDFKVTAVIEADRVIS
                     IESRDTTHKLLGMAFDIGTTTIAGYLVDLKSGKDLCRVSTLNPQTRFGADVISRISYA
                     SESAEGLDKLHGAVINEINNLIQEAAKQANHSPLDVYAITFAGNTTMHHLLLKISPKY
                     LARAPYVPVVTAPVVVDAAEINIRINPEGKVFAFPNIAGFVGGDTVAAALAAELDKAD
                     KLKLLIDIGTNGEIVLGSKGKYLACSTAAGPAFEGVQISCGMRGASGAIDHVSIGEEY
                     EYTVIGNTSPRGIAGSGLVDAVAELFKAGIVDEKGRL"
     gene            complement(1531..2751)
                     /locus_tag="PK768_00930"
     CDS             complement(1531..2751)
                     /locus_tag="PK768_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQA59566.1"
                     /translation="MTKSAQELANERLARIEAAMNLQEPDRVPLRGIGGDIIPAYAGI
                     TQQEFCYDYDKGIKAVEKFLKDFPFDWPSGSIPGLDGRVFSVAFANSPEVAPSTTFIS
                     GPMHDVLGDKYYRFPGNEIDVNATPQFIGGTFMDPEEYDELIEDPVKFVTEKVLPRAC
                     DNLATPRQAMATWVRLGMEFGRFAAAGRQIGMIAASLGYPSVRMGGAYAPLDIIGDFL
                     RGITNTVLDIRRYPDKVKKATEALVEPILNYALTYKKLGINLVMIPLHLNEYLSPKLY
                     KEFYWPTLKEIILKLGEEGVKSWVFFEGHHEQHLETILELPKGWGVAYFEKTDVVKAK
                     QVLQGHTCVVGGIPVSLLVGGTPERIKEYVKNLLEQVKPGGGFMLSPNIGTAPAETPL
                     ENLTALIEAVELYG"
     gene            complement(2758..3387)
                     /locus_tag="PK768_00935"
     CDS             complement(2758..3387)
                     /locus_tag="PK768_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777418.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQA59567.1"
                     /translation="MQEELFNKIVDMDEEGSVKLAKEYLDGGGDPQKLLETCRNAMAV
                     IGDKFEKGEYFLSELILGGEIFTSIMEFTLPHIKEGESKKLGKIVLGTVKDDIHNIGK
                     DIFKAFAEAAGFEVIDIGVDVPVEDFVQQVKEHKPDIIGMSCLITAGITSMKKTVEAI
                     KAEGITDAKIIIGGGRVDEEVKQLAGADAWADDAAKGVRLCKELVGVKE"
     gene            complement(3463..4860)
                     /locus_tag="PK768_00940"
     CDS             complement(3463..4860)
                     /locus_tag="PK768_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA59568.1"
                     /translation="MKKPLSSAIKRFYGIGDFGFTLMTQVELTFFVFFLTDIAKFSLP
                     MVALIGAITSIADAISSPFYGAIISGTKPMKWGRNRSWMLIAPPIVVLLYIFQFTRIG
                     SESMAAFIVCAGFILSHIAWNIPWVANVSLIPVLATNPEERAFLSARRATYTALGTVA
                     FSYTGPPLANFLGQVTNNPILGYSLLAGIMAFVMMVCYWTVFKITEGYEETVQVQTSA
                     AKSASAQGVASIKGMLNSLVQNPPLIVLLISDFLRYMVNFVMAAAAAYYFTYVAQNPA
                     LLSTYLLLGGLAQVVGAHMAATLSKTLSSRNASIFGLFALAASLIICKYVAMNIVMFF
                     VIIMLARIFLGLLTAVMVSLYSDVAVYAQWKTGEDTSPFVMGLMTLSLKTAVISRGTI
                     IPFVLSAAGFVAGVDPATASMAIKNAVINVFVFIPGIFSLVAGLILAFGYKLTKERLI
                     ELQNEINMRKAEVGA"
     gene            complement(5101..6459)
                     /locus_tag="PK768_00945"
     CDS             complement(5101..6459)
                     /locus_tag="PK768_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQA59569.1"
                     /translation="MVNAKQLQQERIRIFHDIYDNKIPKRVPINIGLLFEVIAQFGNL
                     DLKKVLWNPELIYDAADAMCQTVYSDVCPLMSSLRYPSYYKILKSKSFVMASNGFIQH
                     TGVMGMYLEDYDYLIEKPYECIVERVLPRLYKAIDWNEPINSAFSLMKAFYAYNNEFA
                     QANRIIQNLIQNYGYYPQNPLYYGFTQTPFDFLADQLRGFKGISKDIRKIPDKIEKAC
                     EALYPLAFAKGLPKEISKYGYVFIPLHMPAFMREKDFERLYWPTFKRMVDEYASLGLR
                     CNLFCEHDWNRYLDYLYELPQNTILMFEYGDPKLIKQKLGKKHIITGLYPLINLKTKS
                     PEQCADEVKRYMDILAPGGKYIFGFDKSPLSLNDLNIDSLRAVANTVRDYGIYSNPGN
                     NTNFEFDNKKYNAKPSITLKSKYLMSWDYYKLGCKISDFEQQKIKCIEDGIFEFIMFL
                     LL"
     gene            complement(6502..7191)
                     /locus_tag="PK768_00950"
     CDS             complement(6502..7191)
                     /locus_tag="PK768_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HQA59570.1"
                     /translation="MKEMIIACETLKDELLTAMERTGSQLPIIWIDSLYHMEPGMLRK
                     KLQEEIDSVENAENLLLAFGSCGNALIGVKATTANLIIPKTDDCISMVLCKQGEKFER
                     AKKTYFLTKGWIESSRSLTAEYKRAVERYGKEKAERIFKFMLNSYKYLMLIDTQSYDV
                     KKYKGKAEKIAELTNMELIIERGSVWFLEKLLTGPYDDDFCIIPKGEEVKIHHFGYDT
                     DFAPNKSVNLV"
     gene            complement(7365..9017)
                     /locus_tag="PK768_00955"
     CDS             complement(7365..9017)
                     /locus_tag="PK768_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA59571.1"
                     /translation="MKKCKKVTVMLLVVVLFMTFLAPVANAKSWDMPPGQLKKVTGIP
                     YGQLKKIGFLDLDETIEWARMAIDRMYARGIFTGYPGNVFKPRNNVTHLEAIIMSLRV
                     MGWEEEAKRTKVNDDIRKINLLWDDAYYYIALAVEKGLIKAEELRGFNPNAPAKRYEV
                     ARYIVRALDLESQAQKHMKEKLPFKDANAIPKDAVGYVYVMLDLGLMKGDDNNRFKPN
                     DPITRAEMAVMLNRLDERLGQEDENELIGTIRDIDLKNLTITIRNSSGTKTYDLLQET
                     PVYIDGRYRSLEDLRVGDKVELFIDEDEFVIFIQVIEETKQAIVSAKGLVVEVGRRTI
                     SLVTYITEPDEAFIGTLVENDIEGRHYELETKQGTFVLKGNTKGLEDYVGDMIVVKGE
                     ITDEFSIYMRGPLLEVEKYYVLRSRDMVTYDVDGNTKITLDGKTAKLSEITVGDFAQV
                     RADKDNLAIEIKAESCKDKVREREKDGKNTRDGKLEGKVVSVNLGATWQLTIKNDKGQ
                     YTLIIDKDVQLKGLKSLRNLKRGMEIELRIEDGKIIEIELDD"
     gene            complement(9215..10480)
                     /locus_tag="PK768_00960"
     CDS             complement(9215..10480)
                     /locus_tag="PK768_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQA59572.1"
                     /translation="MQKTLMSKKLLALLLIVTLLPASLAFAKEDEKAENEVLKLSLED
                     AIKLAEENNQQVKLSELALEKAKLGRQQYRYQDRKTRDLEDEIRRQIEWWNSLGDEEK
                     QALLEQEGFTPPFDPGITSSFEYTYTMDLLEKQTDVAVKLAEAGIDVTIRGIRFGVEA
                     AYYGAISARDNRLLAEAAVERYQDLLRIAEAKYNAGVVAKTEVLDAQVQLAKAEADLL
                     KAKSQEEKAYINLKKLLGLAQDELIELTDTFDYKPVDFDVTLEELIAEALKNRIDIMS
                     AEGAYEVAKLDFDLNSKVYPSNTFIYKEKQHAMEDARIKLEDTKASVEAEVRQIWLDF
                     EEAKVNIPVLDKALEMAEESLRLARLSYEAGLVRSVDVAAAEEGLRQVQLQRSGVIYN
                     YNLARLKLENVVRFSVSGAAAISSEASPM"
     gene            complement(10465..10845)
                     /locus_tag="PK768_00965"
     CDS             complement(10465..10845)
                     /locus_tag="PK768_00965"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQA59573.1"
                     /translation="MDLPVLNEELNTILSEVTESIKKLYGSKLKKIILYGSYAMGCQD
                     EESDIDLMVLIDMDEPQLRNYDKKLNEIISDIGYRHLKVLSLIDMSYEKYNKWVDVVP
                     YYKNVKNQGVVVYEQKEDFLCKKL"
     gene            complement(10966..>11422)
                     /locus_tag="PK768_00970"
     CDS             complement(10966..>11422)
                     /locus_tag="PK768_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQA59574.1"
                     /translation="MFTVPLALIGVVFALLLSGHNISLISLIGVVMLAGIVVNNAIVL
                     IDYVNQLRERGMSRYEAIITAGPARLRPILMTTLTTVLGLVPLSLGIGEGGELGAPMG
                     VTVIGGLTFSTLLTLIVIPVVYTIFEDWGDILARFKKRIRQKSADAKEA"
BASE COUNT         3038 a         2697 c         2050 g         3637 t
ORIGIN      
        1 taagcggccc ttctcgtcca caatccccgc tttgaagagt tcagccaccg catccactaa
       61 acctgaaccc gcaattccgc gcggggaagt gttgccgatt acggtgtact catactcttc
      121 accaatcgat acatgatcaa tggctcccga ggcgcccctc ataccgcagg atatctgcac
      181 tccctcaaaa gctggccctg ccgcagtaga gcatgccagg tacttgcctt tgctgcccaa
      241 cactatttcc ccgttcgtgc caatatctat taaaagcttt aacttatcag ctttgtccag
      301 ttctgccgcc aaagcagctg ctaccgtatc tcctcctaca aacccagcaa tatttggaaa
      361 tgcaaaaacc ttgccttcag gattaattcg tatgtttatt tcagctgcat ctacaactac
      421 cggtgccgtt actacgggca cgtaaggggc tcgagctaag tactttggtg atatcttaag
      481 caataaatga tgcatagtgg tatttccggc aaaggtaatg gcataaacgt ctaaggggct
      541 atgatttgcc tgttttgctg cttcttgaat cagattattg atttcgttta ttaccgcacc
      601 atgcaatttg tctaagcctt cagctgactc ggaagcatag ctgatgcggg aaatcacatc
      661 tgccccgaac ctcgtttgag gattcaatgt agataccctg cacaaatcct tgccgctttt
      721 taaatccact agataacccg caatggtagt cgtccctatg tcaaaagcca tccctaacag
      781 cttatgtgta gtatcgcgac tttcaataga gatgaccctg tctgcttcaa taactgcagt
      841 tactttaaag tcactctccc ttaatttttc aggcaggtcc tgcagtacct tcagggacgt
      901 tttaatgcct tgttgctcta tgactctttt ccagtcatcc ctgttatcgg aaagagtggg
      961 atgaggcagc tcgaaataca ccttttttac caaagtatca acggctacgt gtcgctcgat
     1021 gccctcagac aatatggcat gtttagcttc cttgtgaggg agtttcaccg ttacatcttc
     1081 ttttaccctg tactggcatg caagggcctc ttggacttgc ccatctgatc taatgatagt
     1141 taccttacac cttttacaga cgcctagtcc cccacaggga aattcaaagt ttataccgtt
     1201 ctcattaaaa acttgtttta aagtaaagtc tgccggtacc ttaatttgta cactgtcagg
     1261 aagtatggtt acattaaaga aatccatcat atcacccctt gtctgactat tataccatta
     1321 ttataacatt ttttaatggt actatttgct gtatgtttca cttcctcccg ttgttcctgt
     1381 tgtagatgca tttttgactc tatagtgcaa aggcagttcc gcataggcgg atagcaaccg
     1441 ctaaaaccaa tgcccggaaa caaccacccg gaaccttctt ttcagagtaa aattttaggc
     1501 gccgatacag cttatcggcg ccctataata ttaaccgtac aattctactg cttctataag
     1561 ggcagttagg ttctccagcg gtgtttcagc aggagctgtt ccgatatttg gtgaaagcat
     1621 gaatcctccg ccgggtttta cttgctccag caaattctta acatattctt ttattctctc
     1681 gggagtgccg ccgaccaaca aacttacagg aataccgcca acaacacagg tgtgtccctg
     1741 taatacttgt tttgccttaa ctacatcggt tttttcaaag tatgctacac cccagccctt
     1801 tggcagttca agtatggttt ccaaatgctg ctcatgatga ccctcaaaga atacccagga
     1861 tttcacacct tcttcgccga gttttaaaat tatttctttt aaggtcggcc aataaaactc
     1921 cttatacagt tttggtgaca agtactcatt caaatgcaga ggaatcatca ctaggttaat
     1981 tcctagtttt ttgtaagtga gagcatagtt caaaataggc tctaccaatg cctcagttgc
     2041 cttcttgact ttatcgggat acctgcggat atctaatacc gtattagtaa ttccccttaa
     2101 aaagtcgcca ataatatcca gaggcgcata ggctcctccc atccttacgg agggatagcc
     2161 gaggcttgca gcaatcatac ctatttgacg gccagcagct gcgaatctac caaactccat
     2221 gccaagcctg acccatgtag ccattgcctg tctgggcgtg gccaaattgt cacatgctct
     2281 cggtaggacc ttttcggtta caaattttac cggatcttca attagctcat cgtactcttc
     2341 gggatccata aaagtgccgc caataaactg cggtgtggca tttacatcga tttcattgcc
     2401 cggaaatcta tagtacttat ctcccagcac atcatgcata ggacctgaaa tgaaggttgt
     2461 acttggagct acctcggggc tgttagcaaa tgccacacta aacacccttc cgtcaagacc
     2521 gggaatggaa cccgaaggcc agtcaaacgg aaaatccttt aaaaactttt caactgcttt
     2581 tatgccttta tcatagtcat agcaaaattc ttgctgggtg atacctgcat atgcaggaat
     2641 tatatctccg cctattcctc taagcggaac cctgtcaggt tcctgaagat tcatagctgc
     2701 ttctatgcgc gcaaggcgct cgttcgcaag ttcctgagca ctcttagtca ccttgtatca
     2761 ctcctttacc ccaactaatt ctttgcacaa ccttactccc ttagccgcat catcagccca
     2821 tgcatctgcc cctgccaact gtttaacttc ttcgtctact ctgccgccgc ctatgattat
     2881 cttggcatcc gttattccct cggcttttat tgcttctacg gtctttttca tcgaagtaat
     2941 accggctgtt attagacagg acattcctat aatgtcaggc ttgtgttctt taacctgttg
     3001 tacaaaatct tctactggaa cgtctactcc gatgtctatt acctcgaagc ccgctgcctc
     3061 tgcaaatgct ttaaatatgt ccttgcctat gttatgtatg tcatctttta ctgtaccaag
     3121 tacgattttg ccaagtttct tcgattcacc ttcttttatg tgcggcaagg tgaattccat
     3181 tatgcttgta aatatttctc cgccgagtat gagttctgac aggaagtatt cccctttttc
     3241 aaatttgtcg ccaattacgg ccatggcgtt tcgacatgtt tcaagtagct tttgaggatc
     3301 tcctccacca tccaaatact ctttggctag ttttactgaa ccctcttcgt ccatatcaac
     3361 tattttattg aaaagttcct cttgcatatg ttttcctcct ttttgtattt attgttttat
     3421 tcttaaaatt ttacaggcac ccccccttga ctataaattt atttacgctc caacctccgc
     3481 tttcctcata tttatttcgt tttgtaattc aattaatctc tcttttgtta acttgtaccc
     3541 aaacgcaagt atcaatccag ccactaagga aaagataccc ggtatgaaca caaacacatt
     3601 aattacagca tttttaatag ccattgacgc cgtagccggg tctacaccgg caacaaatcc
     3661 agctgccgac agaacaaatg ggatgatggt tcccctggaa ataactgctg ttttaagtga
     3721 tagggtcatt aatcccatga caaatgggct ggtatcctct ccggtcttcc actgggcata
     3781 taccgcaaca tctgaataga ggcttaccat aaccgctgtc aaaagaccaa ggaatatcct
     3841 agccagcatt ataattacga agaacattac gatattcata gctacgtatt tacagattat
     3901 caatgacgct gctagggcaa acagaccaaa aatcgatgcg ttcctagacg aaagcgtctt
     3961 tgaaagagta gctgccatgt gcgcacccac aacttgtgcc aaaccaccca gtagcaaata
     4021 tgttgataac agggctgggt tttgcgctac ataggtaaag taataagcag ctgccgcagc
     4081 cattacaaaa ttaaccatat atcttagaaa gtcggaaatc agcaggacaa tgagaggagg
     4141 attctgcacg agactgttca gcattccttt tattgatgct acgccttggg ctgaagcact
     4201 ttttgccgca gatgtttgca cctggacggt ttcttcgtag ccttcagtta ttttgaaaac
     4261 cgtccaataa caaaccatca taacaaaggc cattatgccg gcaagtagag agtatcctaa
     4321 tataggattg tttgttacct ggccaagaaa attggccaag ggaggacctg tgtaagaaaa
     4381 ggcaactgtc cctaatgcag tgtatgtagc acgtctggca gaaagaaagg cacgttcttc
     4441 aggattcgta gccaataccg gtatcagcga aacgttcgca acccatggaa tattccaagc
     4501 aatatgactt aaaataaaac ctgcacatac aataaaagct gccatagatt cggagccgat
     4561 tctggtgaac tggaaaatgt ataaaagaac tacaatgggt ggcgcaataa gcatccaaga
     4621 acggttcctg ccccatttca tcggttttgt gccgcttatt atggcaccat aaaacggaga
     4681 tgatatagca tccgcaatac ttgtaatagc tccgattagg gctaccatgg gaagggaaaa
     4741 ctttgctatg tcagtaagga agaaaacaaa gaaggtcaat tctacttgag tcattaatgt
     4801 gaaaccaaaa tcaccaatgc cataaaacct tttgatggca ctgctcaacg gctttttcac
     4861 ggtttctacc cctcctttgt atgatttaga tatttaaata attattaaac tgttttatta
     4921 aatcacctcc catttaaaga cttaaattgt attgtatata catgtagact tatttttagt
     4981 atatataaaa taaacgtatt tgtcaataca atttagaaac ttttatatta tgtacaatat
     5041 ataggcaatg tgatatattc tgtcaattct gcctaaataa tgacattaaa atacataatt
     5101 ctacagcaat agaaacatta tgaattcaaa tatcccatct tcaatacatt ttattttttg
     5161 ctgttcaaaa tcagatattt tgcaacctag tttataatag tcccacgaca ttaagtattt
     5221 gcttttaaga gttattgaag gctttgcatt atatttctta ttatcaaatt caaagtttgt
     5281 attgttcccc ggattactat aaataccgta atccctgacc gtgttggcaa cagctcgcaa
     5341 tgagtcgatg ttcaaatcat ttaacgataa aggactttta tcaaagccaa atatgtattt
     5401 accgcccgga gcgaggatat ccatgtacct tttgacttca tcggcacatt gctccggaga
     5461 ttttgttttt aaattaataa gtggataaag accggtaata atatgttttt tgcccaattt
     5521 ttgttttata agtttgggat ccccgtattc aaacataagg atagtattct ggggcaattc
     5581 ataaaggtaa tccaaatacc tgttccaatc atgttcgcaa aatagattgc aacgaaggcc
     5641 aagggaagcg tactcatcca ccattctctt aaaagtcggc caataaagcc tttcaaaatc
     5701 tttttccctc atgaaagcag gcatgtgcag tgggataaat acatagccgt atttagaaat
     5761 ctccttgggc agacccttag caaaagccaa aggatataag gcttcacagg ctttttcaat
     5821 tttatcggga atcttcctga tatccttgct aattccttta aatcctctca attgatcagc
     5881 taaaaaatca aagggcgttt gggtaaagcc gtaatacaat ggattttggg gataataacc
     5941 gtaattctgg atcaaatttt gtattatcct atttgcctgg gcgaactcgt tattataggc
     6001 gtaaaatgcc ttcatcaggc taaatgcact attgattggt tcgttccagt caatagcttt
     6061 atatagtctg ggtaaaaccc tttctacaat gcattcatac ggtttttcta tcagataatc
     6121 atagtcttct aaatacatcc ccataactcc agtatgctga ataaagccgt tagatgccat
     6181 gacaaaagac ttggatttaa gtattttata ataagagggg tatcttaacg agctcatcaa
     6241 gggacacaca tctgaataca cagtctgaca catggcatca gctgcatcgt aaatcaattc
     6301 gggattccaa aggacttttt tcaggtccaa gtttccaaac tgtgctatta cttcgaagag
     6361 aagacctata ttaatgggaa cccttttagg aattttgtta tcgtagatat cgtgaaaaat
     6421 ccggattctt tcttgctgca gctgttttgc attcaccata tttcacctcc cccgcatagt
     6481 aatattaaag attcaagggt attaaaccag atttaccgat ttgtttggag caaaatcagt
     6541 atcatatcca aaatggtgta ttttaacttc ctcccctttt ggtatgatac aaaagtcatc
     6601 gtcgtacggg ccggttagca gtttttctaa aaaccaaaca cttccccttt caatgataag
     6661 ctccatattt gtcaactcgg caatcttttc agctttgccc ttgtattttt tcacatcata
     6721 agattgggta tcgatgagca tcaggtactt atagctgttg agcatgaatt tgaaaatcct
     6781 ttccgccttt tccttgccgt acctctccac cgctctcttg tattccgctg ttaaacttct
     6841 tgaagactca atccagcctt tcgtcaaaaa gtaagtcttt ttcgcacgct caaatttttc
     6901 accctgtttg cacaatacca ttgaaatgca atcatcggtc ttagggataa tgagatttgc
     6961 agtagtggcc ttgactccga tcaaagcatt tccgcaactg ccaaaagcaa gtaaaagatt
     7021 ttccgcattt tccacggaat ctatttcttc ctgaagtttt ttgcgcagca tcccgggttc
     7081 catatggtac aatgaatcta tccaaataat aggtagttga gatcctgtcc tttccattgc
     7141 cgtaagcaac tcatctttta atgtttcgca tgcaataatc atttctttca tggtgttacg
     7201 ctcctttgtc agtttttacg atttgcttat tatactataa aattttataa caattttgtc
     7261 aatgaaaagg tatttaagaa aaatgccccc ttaatgtgac aggattgctg ccccgccaca
     7321 ttaaggggac acatttaagt attgattctc tataaattta ttatttaatc atcaagctca
     7381 atctctatga tttttccgtc ctctatcctt agttcaattt ccatgcccct cttgagattc
     7441 cttaagctct ttaacccttt aagttgcaca tctttatcta tgattaatgt gtactgacct
     7501 ttgtcgtttt ttatggtcag ctgccaagtt gcgcccaagt tgaccgacac cactttgcct
     7561 tctaacttgc catctcttgt gtttttgccg tctttctccc gttctctgac tttgtcttta
     7621 caactctccg ccttgatttc tatggcaaga ttgtctttat ccgcacgaac ttgcgcgaaa
     7681 tctcccacag ttatttcaga tagtttggct gtcttgccgt ccagggttat ttttgtattc
     7741 ccgtctacat catatgttac catgtcccgg gaccttaaca cataatattt ttctacttct
     7801 agcaatgggc ccctcatgta aatggaaaat tcgtctgtaa tttctccttt tactacaatc
     7861 atatctccaa catagtcttc aagccctttt gtgttgcctt ttaatacaaa tgtgccctgc
     7921 tttgtttcca attcatagtg acggccctct atgtcatttt caacaagggt acctataaag
     7981 gcctcatcgg gctccgttat atatgtcacc agggaaattg tccttcggcc cacctctacc
     8041 acaaggcctt ttgctgagac tatggcctgc ttggtctctt caatgacctg gataaatatc
     8101 acgaattcat cctcatcgat aaatagttcc accttatctc caactcttaa gtcttctaag
     8161 ctgcggtacc tgccatctat atagacggga gtctcctgca gaaggtcata ggtctttgtg
     8221 cctgaagaat ttcgtattgt tatggttaaa ttcttcaagt caatatctct tatggtgccg
     8281 attaattcat tttcatcttc ctgcccgagg cgttcatcca ggcggttgag cattactgcc
     8341 atttcagccc tggttatggg atcatttggc ttaaacctgt tgttgtcgtc acctttcatc
     8401 agccctaagt ctagcatcac gtatacgtag ccaacggcat ccttggggat ggcattagca
     8461 tctttaaagg gaagtttttc cttcatgtgt ttttgtgctt gactttccaa atccaatgcc
     8521 cttacgatgt atcttgccac ttcatagcgc ttggctggtg cgttgggatt gaagcccctt
     8581 aattcctcag cttttatcag gcctttttca acagccaggg ctatgtagta ataggcatca
     8641 tcccacaaaa ggttgatttt tctaatatca tcgttaacct tagtcctttt agcctcttct
     8701 tcccatccca tgaccctaag ggacattatg atggcttcca agtgagtcac attgttcctg
     8761 ggcttgaaga cattacccgg gtaccccgta aatatcccgc gggcatacat ccggtcaatg
     8821 gccatcctgg cccactcgat tgtctcgtct aaatcaagaa agcctatttt tttaagctgc
     8881 ccatagggta tccccgtcac tttttttaac tgaccgggtg gcatatccca gctttttgca
     8941 ttagccacag gggctaggaa ggtcatgaat aaaaccacga ctaaaagcat cactgtaact
     9001 tttttgcact tcttcattga taccctcctc ttttgaatat ggaccctgtt tccggaaagc
     9061 aaggttttgt tttatctatc atataatatc gaaggagagt cccctattat gagggtatct
     9121 cggaattgta accggattgt aacactattg taactttcgg gtaatatgcc aagtcaggtt
     9181 tggctttttt cattatactt gtgccgtaaa atacttacat gggactagct tctgatgaaa
     9241 tggccgcagc tccggataca gaaaagcgca ctacgttttc aagtttcagc ctggccaagt
     9301 tgtaattata tatcactccg gacctttgaa gctgcacctg tcttaggcct tcctcggcag
     9361 ctgccacatc aacgctccga acaaggcccg cctcgtagct gagccttgca aggcgaaggc
     9421 tctcttctgc catctccaaa gccttgtcca aaacaggtat gtttactttg gcttcttcaa
     9481 agtcaagcca tatttgacgc acttctgcct caacagacgc cttggtatct tctaatttta
     9541 ttcgggcgtc ttccatggca tgctgttttt ctttgtatat gaaagtattt gagggataaa
     9601 ctttgctatt taagtcgaaa tccagttttg ctacctcata ggctccttct gcgctcataa
     9661 tgtcaattcg gttctttagt gcttctgcta tcagctcttc taatgtcaca tcgaaatcta
     9721 caggcttgta gtcaaaagta tcggtaagct ctatcagctc atcctgggcc agccccaaga
     9781 gtttcttgag atttatgtaa gccttttctt cctgcgactt ggctttaagc aaatccgctt
     9841 cggcctttgc caactgcacc tgggcgtcta aaacttcagt ttttgcaact actccagcat
     9901 tatattttgc ttcggcaatc ctcagcaaat cctgatatct ctcaacggca gcctctgcta
     9961 aaagcctgtt atcccttgcg gaaatggcac cgtagtaagc ggcctctaca ccaaaacgaa
    10021 tccctctaat ggtcacatcg atgcccgcct cggcgagttt aactgctaca tctgtttgtt
    10081 tttctaaaag gtccattgtg tatgtatact caaaacttga tgttataccg gggtcgaatg
    10141 gtggcgtaaa tccttcttgc tctaggagtg cttgtttttc ttcatcacca agggaattcc
    10201 accactctat ctgccttctt atttcatctt ccaaatcccg agttttcctg tcctgatacc
    10261 tatactgctg tcttcccaat ttggccttct ccaaggccag ttctgacagc tttacctgct
    10321 gattattctc ttcggcaagt tttattgcat cctcaaggga aagtttcaag acttcatttt
    10381 cagccttttc gtcttctttg gcaaaggcga gacttgcagg taacaatgtc actatgagta
    10441 atagagccag taattttttg ctcatcaaag ttttttgcat aagaagtcct ccttctgttc
    10501 atatacaaca acaccttggt tttttacatt tttataatat ggtactacat ctacccactt
    10561 attatatttt tcgtaactca tatcaattaa tgacaggacc ttcaaatgac ggtaccctat
    10621 atcgcttatt atttcattga gctttttatc gtagtttcgc agttgaggct cgtccatgtc
    10681 gattagtacc atcagatcaa tatcggattc ctcatcctgg catcccattg catatgaacc
    10741 gtacaaaatg atttttttaa gtttagaacc atacagcttt ttaatacttt cagtaacttc
    10801 actaaggata gtatttaatt cttcatttaa aacaggaaga tccacggttt cacctctttt
    10861 cctgttcatt aactaaaatc attatagcaa tactttatta taaaaacaat tttaataata
    10921 tgccgcctac caggattatt atggcaggcg gctttgccgg ctctgttaag cttccttagc
    10981 atccgctgat ttttgtctta tcctcttttt aaaccttgcc aaaatatctc cccagtcctc
    11041 aaaaatcgtg taaactaccg gtatcactat caaggtaagc agggtggaaa aggtcaaacc
    11101 tccgataacg gtaacaccca taggggcacc cagttctcca ccttctccta ttccgagaga
    11161 caggggcacc aagcctaaga cggttgtcaa agttgtcatc aggatgggcc tgagccttgc
    11221 cggtcctgcc gtgataatag cttcgtacct ggacataccc ctttctctga gttggttgac
    11281 gtagtctatc aggactatgg cattgttgac aacaattcct gctagcataa ctactcctat
    11341 taatgaaatc aagcttatgt tatgacctga tagaagcaat gcaaaaacaa ctcctatcaa
    11401 agccaaggga acagtaaaca tg
//
LOCUS       DAONUO010000013        11357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4409_length_11357_cov_3.896567, whole genome shotgun sequence.
ACCESSION   DAONUO010000013 DAONUO010000000
VERSION     DAONUO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4409_length_11357_cov_3.896567"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK768_00975"
     CDS             complement(<1..460)
                     /locus_tag="PK768_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA59575.1"
                     /translation="MINKELVVIGGGPAGLAAAIEAKKNGVEDVLLIERDSRLGGILP
                     QCIHNGFGLHIFKEELTGPEYAHRFIEELEDLNIEVLLDTMVIDLNENKQLTAVNREK
                     GLMQIQADAIILTMGCRERTRGAINIPGFRPAGIFTAGTAQRFVNIEGYMP"
     gene            complement(450..1922)
                     /locus_tag="PK768_00980"
     CDS             complement(450..1922)
                     /locus_tag="PK768_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA59576.1"
                     /translation="MEDIVIIGAGVTGCSIARELSKYDLSITVVEKEEDVACGTSKAN
                     SAIVHAGFDAAPGTWKAKLNVIGNRLFPALCEELDVPFVMNGSLVVAVTDEEEQALEE
                     LLERGRINGVEKLRIIGKDELLDLEPNLSPNVRGALDAPTGGLVCPYELVIALAENAN
                     QNGVKFWLNAPVTDIEVLEDNSFLVKTPKGSIQTKYVINAAGLFADEISKMAGAEEYS
                     ITPRKGEYLIFDKQFGNLVKKAIFPTPTKISKGILVCPTVDGNIFIGPNSNNIEDKHD
                     TSVNVPGIEEIIAGGKKLVPDLPLKNVITSFAGLRAVSNTNDFIIEASKLVKGFINVG
                     GIQSPGLTSAPAIALMVKDILQEEGLTLKEKADFIPHRPKKVRFREMSNEERSKLIKE
                     NPAYGRVICRCETVTEGEIIDAIRRPVGARSIDAVKRRTRAGMGRCQGGFCSPRVLEI
                     LMRELGADAIDITKKGPGSNILMGRIKEFLQNEGGDDNDK"
     gene            complement(2038..3531)
                     /gene="glpK"
                     /locus_tag="PK768_00985"
     CDS             complement(2038..3531)
                     /gene="glpK"
                     /locus_tag="PK768_00985"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQA59577.1"
                     /translation="MAKYVMALDQGTTSSRAIIFDENGTIKGVTNREFTQIYPKPGWV
                     EHDPMEIWGSQIGVAKEVLEKSGLSPSDIAAIGITNQRETTIVWDKNTGKPVYNAIVW
                     QCRRTAPICDELKEKGLTEKIREKTGLVIDAYFSGTKVKWILDNVEGAREKAEKGELL
                     FGNVDTWLIWNLTGGKVHVTDYSNASRTMLFNIHTLDWDDEILAELNIPRAMLPKPMP
                     SSHVYGYTTTEVFGGEVPIAGDAGDQQAALFGQACYSPGMAKNTYGTGCFMLMNTGEK
                     AVASESGLLTTIAWGVNGKVEYALEGSIFIAGAVVQWLRDELRILDNAAQSEELATKV
                     EDNNGVYLVPAFVGLGAPYWDMYARGTIVGLTRGAKREHIVRAALESICYQTRDVLEA
                     MQKDSGITLKSLKVDGGAVANNFLMQFQSDILGVPVDRPKVIETTALGAAYLAGLAVG
                     YWKDKDDIAAKWQIDRTFEPEMDSETRDNLYKGWKKAVERALDWEDK"
     gene            complement(3686..4024)
                     /locus_tag="PK768_00990"
     CDS             complement(3686..4024)
                     /locus_tag="PK768_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59578.1"
                     /translation="MTFPKIIATIAGGFIFPFLIRLCWGKLVEHLGPIGGWIAAGFIV
                     GTTWTLNHGVGLIYQSGAAWIDMAWAAFIGLFVASALSGDDVGKGLGTVINAILGGIL
                     GGFILYCLYV"
     gene            complement(4059..4442)
                     /locus_tag="PK768_00995"
     CDS             complement(4059..4442)
                     /locus_tag="PK768_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59579.1"
                     /translation="MKNIGSIIGFAIAGIFVMSVWGAFAEAYGIFGGWFAGLAIISIM
                     WFMNHFLELVANEGAFVDIAAGIAVTGTLRDVFLNGPQAGIDSLPTLAFVAIGAVLAG
                     VTAVAIEKMWAERDAAVSAKEDISA"
     gene            complement(4586..5194)
                     /locus_tag="PK768_01000"
     CDS             complement(4586..5194)
                     /locus_tag="PK768_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HQA59580.1"
                     /translation="MDDVLNDLIMSPVIPAIRDIERIDLALSKKSRCIFILTGNILNI
                     KDVVNRAKSAGKRVFLHVDLLEGISKNGMGMKYIAQEIKPDGILTTRSNLVSCAKAEG
                     LFTIQRIFVLDSLAIDTATKSVKHVTPNAIEILPAVIPKVIRRICQRVSYPIIAGGLI
                     EEESEVKAALQEGAIAISVSNENIWNIPFEDLEMWRKSFLGK"
     gene            complement(5392..5988)
                     /gene="plsY"
                     /locus_tag="PK768_01005"
     CDS             complement(5392..5988)
                     /gene="plsY"
                     /locus_tag="PK768_01005"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQA59581.1"
                     /translation="MIKTIVYIIVSYLIGSISFAYYICSKFYSIDIRQYGSGNPGSTN
                     VLRVLGAKPALIVFVADLLKGLITVSIGRLIGGENLALLAAIAVVVGHDWSIFLNFKG
                     GKGIATSLGVVIGVFPKVAPILIIVGISVIFISRYVSLGSITTATLLPILLIIFKYPA
                     KHIIVGLIIGAIAVYRHRDNIERLLAGKENKLGQKVKR"
     gene            complement(6160..6570)
                     /locus_tag="PK768_01010"
     CDS             complement(6160..6570)
                     /locus_tag="PK768_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HQA59582.1"
                     /translation="MNIAVVDGMGGGIGSQIISLLRQDLPSDIKIYALGTNSAATSNM
                     MKEKANKGATGESAITFAVNKVDVIVGSLGIIIPNSMLGEITPSIATAIASCNAKKIL
                     IPIIDTDDITLLGIEKKPLFLAIKDAIEKIKELL"
     gene            complement(6567..6764)
                     /locus_tag="PK768_01015"
     CDS             complement(6567..6764)
                     /locus_tag="PK768_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HQA59583.1"
                     /translation="MCESKVYILEDGQEKKLMDNVIHIEPQDGKLFMYDLLGEQKIVD
                     AVIKEIKLLEHKIILEREKSK"
     gene            complement(6782..7027)
                     /locus_tag="PK768_01020"
     CDS             complement(6782..7027)
                     /locus_tag="PK768_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59584.1"
                     /translation="MSEHNCCRGCGGRASKEKDVETLRILLAHWIEHNNSHEQGFREW
                     AEKAEKIGNAKVYEMITKAADSLKAASDYLLEAKKHI"
     gene            complement(7175..9103)
                     /gene="acsV"
                     /locus_tag="PK768_01025"
     CDS             complement(7175..9103)
                     /gene="acsV"
                     /locus_tag="PK768_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HQA59585.1"
                     /translation="MDKCIVTFLPIGKAVEVAKGTNLMEAARKAGVFLDAPCGGKGHC
                     GKCRVTVKSGQHKHEYTPILTEDEIQQGTRLACLTWVQEDMTVKLMEVDVVNDIMVEN
                     ITSRASTKLVNRAIDILKDSGIKVGTSFKIVPVKLPEPTIDDNIPDLERVQRELKSVL
                     GCTMVNWTIDALRKLPVLLRDSDYGITLTLLKNGGGYDVINVSAESKTSSYGLCVDVG
                     TTTVAACLVDIETGEIKSSANSGNLQMQYGGDIINRIIYSTKENGLEKLNHAIVEGTV
                     NQLLREMIKELEITQDEIVFSVFAGNTTMAHLLLRVYAENIRLEPYIPAFRNAPALKA
                     GDISLGINKNAPVFILPNVASYVGGDIVAGVLASGLWNGDETVLFIDLGTNGEIVLGN
                     KEWMLTCACSAGPAFEGGEISCGMRAASGAIDEVRIDRQNLRPVVRVIGDTKPKGICG
                     SGIIDLIAEMFLKGIIDMKGKINQNIKSDRIRYNQHFGCFEYVVAFGSETKDGLDITI
                     NEIDIDNFLRAKGAVYSGAKTLLNNIGMDVSDIDRVIIAGGIGQNLDINNSITIGLLP
                     DLPHEKFEFIGNSSLTGAYACLVSDDAREKVKEIADSMTYIELSADPAYMEEFISACF
                     LPHTDTSLFPSLKKAMNL"
     gene            complement(9203..9580)
                     /gene="gcvH"
                     /locus_tag="PK768_01030"
     CDS             complement(9203..9580)
                     /gene="gcvH"
                     /locus_tag="PK768_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQA59586.1"
                     /translation="MEFPGDLKYHQEHTWAKVEGDVATVGITDYAQDQLGEVLFVDLP
                     EVGDTVTQGEPFGSIESAKVASDLYAPISGEIIEVNEALDDEPELVNSSPYEDGWIIK
                     VKVSDPAEEANLLSSSDYEKTLE"
     gene            complement(9601..>11357)
                     /gene="acsB"
                     /locus_tag="PK768_01035"
     CDS             complement(9601..>11357)
                     /gene="acsB"
                     /locus_tag="PK768_01035"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HQA59587.1"
                     /translation="GVPLVTGDIPGVAVVIGEAPTAEEAAKIIKDYQSKGILVTLVGK
                     VIDQAIEAGVKIGLDFRVVPLGYDVTSVIHIVSFAIRAALIFGNVTPGDLAELLKYTK
                     ERVPAFVNALGPLSELVVSAGAGAIALGFPVLTDQDVQEVPMNLIVQKDYDKLVQSSL
                     EARGIKIKVTEIPIPVGFAPAFEGERVRKDDMFAEFGGGRTTAWELVKMREMSEVEDH
                     KIELIGPDVDTLGPEGGKLPLAVLVEVAGKNMQEDFEPVMERRIHYFMNYIEGVMHIG
                     QRDIAWVRISKSSYEAGFRLKHIGEVMYAKMLDEFGSIVDKVQVTIITDKEKVEELLN
                     EVARPRYNARDARLAGLTDESVDTFYSCLLCQSFAPAHVCIVTPERLGLCGAVSWLDA
                     KATHELDPTGPCQPIVKGECLDERKGHWASVDKAVTEQSNGATTSVNIYTIMEDPMTS
                     CGCFECICGIMPEANGVIIVNREYHGMTPLGMTFGELASTTGGGVQTPGFMGHGRQFI
                     TSKKFLYADGGLQRVVWMPKELKEALKEKLIERGKEIGIENFYEMIADESVGTDPDTV
                     VEFLTNVGHPALTMDPMF"
BASE COUNT         2842 a         2783 c         2233 g         3499 t
ORIGIN      
        1 caggcatgta tccttcaata tttacaaacc tttgggcggt gccagcagta aatatccctg
       61 cgggtctgaa accgggaata ttaatagctc ccctggtcct ttccctgcat cccatggtga
      121 ggattatcgc atctgcttga atctgcatca atcccttttc gcggttgact gcagtgagct
      181 gtttattttc gttaaggtca atcaccatcg tatcaagcag aacttctata tttaaatctt
      241 ctaattcctc aataaatcta tgtgcatatt caggaccggt aagttcttct ttaaaaatat
      301 gaaggccaaa cccattgtga atacattgtg gcaggatacc gcctaagcgt gaatcgcgct
      361 caattagtaa aacgtcttct actccgttct ttttcgcttc gattgccgcg gcaaggccgg
      421 caggaccacc gccgattact accagttcct tatttatcat tgtcgtcacc gccttcgttt
      481 tgtagaaatt ctttgatacg acccattagt atattagaac ctggtccttt tttggttata
      541 tcaatggcat ccgcacccag ttcgcgcatt aaaatctcca gtactcttgg tgagcaaaaa
      601 ccaccctgac atcttcccat gcctgcacga gtcctcctct tcacagcatc tatgctgcgt
      661 gcgccaactg gtctccttat ggcatcgata atttcccctt cggtaacagt ttcacagcga
      721 cagatgacgc ggccgtatgc ggggttttcc ttgatgagct tgctgcgctc ttcgttactc
      781 atttctctaa accgtacctt cttcggtctg tgcggtataa aatctgcttt ttcctttagg
      841 gttaagccct cttcctgcaa gatgtccttt accatgagag ctatggccgg tgcagatgtt
      901 aagcctggtg attgaatgcc gcccacgttg ataaagccct tcaccagttt agaagcctcg
      961 attataaaat cattggtatt tgataccgct ctcaggcccg caaacgaggt tattacattt
     1021 ttcagaggca aatccggcac aagttttttt ccgcccgcta tgatttcttc gatgcccgga
     1081 acatttacgc tggtgtcatg tttgtcttcg atgttatttg agttaggacc aatgaaaata
     1141 ttgccatcaa cggtgggaca taccagtatt ccctttgaaa tcttggttgg cgttgggaat
     1201 atggcctttt ttaccaagtt cccgaattgc ttatcaaata tcaggtattc gccctttctt
     1261 ggagttattg aatactcttc tgctccagcc atttttgata tttcatcagc gaatagacca
     1321 gcagcattta ttacatattt ggtctgaata gatcctttcg gggtcttcac taaaaagcta
     1381 ttgtcttcta aaacctctat gtccgtcaca ggtgcattta gccagaattt aacaccgttt
     1441 tgattggcat tttcagcaag agctataaca agttcatatg gacataccag cccacctgtt
     1501 ggagcatcga gggctcctcg aacatttggg ctcagatttg gttctaaatc aagcaattca
     1561 tcttttccga tgatgcgaag cttttctact ccgttaattc gccctcgctc taacaactct
     1621 tcaagggctt gttcttcttc atcggttacc gcgactacta gagaaccgtt cataacaaag
     1681 ggaacatcaa gttcctcaca caaggcagga aaaagcctat tgcctataac attcaattta
     1741 gctttccaag taccaggggc tgcatcaaag cccgcatgga ctatggcgct gtttgctttg
     1801 cttgtaccgc acgccacatc ctcttctttt tccactacgg taatgctcaa atcatacttt
     1861 gacagttcgc gagctataga gcaacccgtt acgcctgctc caataattac tatgtcttcc
     1921 atattctacc tccctaaaaa tgggaaaacc acacctatat aaaggtgtgg ttctccttat
     1981 tctccgccac agctttaaat gcttaagaaa ttttttaagc ttttgctttg acaagtatta
     2041 tttatcttcc caatcgagag ctctttcgac agctttcttc caacccttat ataggttatc
     2101 tcttgtttca ctgtccattt ccggttcaaa ggtcctatcg atctgccact tagctgcgat
     2161 gtcatctttg tctttccagt atcctactgc aagaccagcc agatatgcag cacctaaagc
     2221 ggttgtttca attactttcg gtctgtcaac gggtacgcct aagatgtcag actggaattg
     2281 catcaggaag ttgttagcaa ctgctccgcc gtctaccttg aggctcttca gggttatgcc
     2341 ggagtctttc tgcatggctt cgagaacgtc tcttgtctga tagcaaattg actcaagtgc
     2401 agctctgacg atatgttcac gctttgcacc gcgggtgagg cctacaattg tgccgcgagc
     2461 atacatatcc cagtaaggag cacctagacc tacaaaggcg ggtaccaggt atactccgtt
     2521 gttgtcttct acttttgttg caagctcttc actctgggct gcattgtcga gaattctgag
     2581 ctcatctctg agccactgta ctacagcacc agctatgaag atactgcctt caagggcata
     2641 ttctactttg ccatttacac cccatgctat ggtggtcagc agtccgctct cggaagcgac
     2701 agctttttca ccagtgttca tcaacatgaa gcatccggtt ccgtatgtgt tcttcgccat
     2761 acccgggctg tagcaggcct gaccgaacag tgctgcttgc tggtcaccgg cgtcaccggc
     2821 aatcggaact tcgcctccga atacttctgt ggtggtgtat ccgtatacat ggcttgaggg
     2881 cattggtttc ggaagcattg cgcgaggtat gttgagttct gccagtattt catcgtccca
     2941 atccagcgta tggatattaa agagcattgt tcttgaggcg ttactgtagt cggtgacgtg
     3001 aaccttgcca ccggtcaggt tccaaatgag ccatgtgtca acgttaccga atagcaattc
     3061 gcctttttct gctttttccc tggcaccttc cacattgtcg aggatccatt ttacttttgt
     3121 gccggagaaa taagcgtcaa ttacgagacc ggtcttttcg cggatttttt cggtgagccc
     3181 tttttctttc agctcgtcac aaataggagc cgttctgcgg cactgccata caatggcatt
     3241 gtaaacgggt ttgccggtat tcttatccca tacaatggtg gtttcgcgct ggttggttat
     3301 acctattgct gcaatatcgc ttggagacag accgcttttt tcaagaactt ccttggccac
     3361 gccaatctgg cttccccaaa tttccattgg gtcgtgttct acccagccgg gcttggggta
     3421 aatctgggtg aattcgcggt tggttacacc cttaatggtt ccgttctcat caaagatgat
     3481 cgcccttgaa ctggttgtcc cctggtctaa agccattaca tacttagcca ctaaacattc
     3541 ctccttgttt ttataatatt attttagatt tatgtgtgcg gtgcggccta ggccgcacca
     3601 aaatttccta atgcggcact tttgttacta ttaactttta tatattattt tgcagtcagt
     3661 aaggatatta atatttttct gacaatcaaa catacaagca gtagagaata aatccgccta
     3721 gaattcctcc aagaattgca ttaataaccg tccctaaacc tttgcctaca tcgtcaccgg
     3781 atagggctga agctacaaac aagccaataa atgctgccca ggccatgtca atccaagctg
     3841 ctccggattg atagattaga cctactccgt ggttgagtgt ccatgtggta ccgactataa
     3901 acccggcagc gatccaaccg ccaataggtc ctaaatgttc aacaagtttt ccccagcata
     3961 gtcttatgag aaatgggaat ataaaaccac cagctatagt cgctataatc tttggaaatg
     4021 tcatttatat acctccccct tattttttca tgataagcct acgcggaaat gtcttctttt
     4081 gctgaaacag cggcatctct ttctgcccac attttttcaa tggcaaccgc tgttacaccg
     4141 gcaagtacag caccaatagc tacaaaagct aatgtaggca gtgagtcgat tcctgcttgc
     4201 ggaccattga gaaatacgtc cctaagagtg cccgtcactg caataccggc tgcaatgtcg
     4261 acaaaggcgc cttcgttagc aacaagttct aggaaatggt tcataaacca cattattgaa
     4321 ataatcgcaa gcccggcaaa ccagcctcca aaaataccgt atgcctctgc aaaagccccc
     4381 catactgaca ttacgaatat accagcaata gcaaatccaa taatagaacc gatgtttttc
     4441 acaaaatgtc acctccaagt tattttggtc ctaagaagtt gtaacctatt aataactcaa
     4501 gagccctttt gtgaacaccc ccctcctttt tgggcaaatt aaaaatcctt ccaaggcaaa
     4561 taaagacaac acaatagtag tgtacttatt tacccaggaa ggactttctc cacatctcca
     4621 agtcctcaaa aggtatgttc catatatttt catttgacac cgagatggct atggcacctt
     4681 cttgaagtgc cgccttcact tcactttcct cttcgataag gcctccggcg attatcgggt
     4741 aagaaaccct ttggcaaatc cttctgataa ctttcggtat taccgccggt aggatttcaa
     4801 tagcattggg ggttacatgc tttacggact tagtagcggt atcaatggcg agactgtcaa
     4861 gtacgaatat tctttggatg gtgaaaaggc cttcagcttt tgcgcaacta acgagatttg
     4921 atctagttgt aagaattcca tccggcttaa tctcttgggc tatatatttc atgcccatac
     4981 catttttgct aatgccttca agtaaatcga catgtaggaa aactcttttg cctgcgcttt
     5041 tagctcgatt tacaacatcc ttaatattca gtatattgcc ggtcagaata aatatgcatc
     5101 gagatttctt ggatagagct aagtcgattc tctcgatatc tcgtattgcc ggtattactg
     5161 gtgacatgat caaatcattc aaaacatcat ccaaggaatc acctctagat tacacagtaa
     5221 tacctgcaaa caccccatta tgtgtttgta aatatccact tttatattat tgcaaaatct
     5281 gtgccaaatt gacaaatgca gttaattggg catttacagg ttgcggacat ggtaaaaagt
     5341 atcagatatg atatgcgttt ttgcagttat ttgatacttt ttaccaattg gtcatctctt
     5401 taccttttgg cctaatttgt tttccttacc agcaagcagc ctctctatgt tgtccctgtg
     5461 gcgatatacg gctatcgctc ctataattag gccaactatt atgtgttttg ccgggtattt
     5521 aaatattata agaagtatgg gtaacagagt tgctgtagta attgaaccta acgaaacgta
     5581 gcgggatatg aatataactg aaatgccaac tattataagt atgggagcca ctttcggaaa
     5641 gacgccaatg acaactccta agctagtggc tatgcccttg cctcccttga agttgagaaa
     5701 gattgaccag tcgtgaccta cgactaccgc aatggcagca agcagggcca ggttttctcc
     5761 acctataagt ctgccgatgg ataccgttat taggcctttt aacaaatctg ccacgaaaac
     5821 tataagggct ggctttgctc ctagcacgcg aagaacattg gtggagccgg gattcccact
     5881 accgtattgg cggatatcga tggagtaaaa tttgctgcag atataatacg caaaggagat
     5941 ggaacctatc aggtaactta ctataatata tacgatagtt tttatcaaga cgattcttca
     6001 cctttctgaa gagatttttc acaatttcta attcacttaa tattatacac ttcatgttgc
     6061 atgaagtcaa tgattttttt aaaaaaagtt ggtacttttt tacctttctc cctgaaacaa
     6121 aaaagccgct gcattgcggc atgatgattt ttacaagttc tacaatagtt cttttatttt
     6181 ttctatagca tctttgatcg ccaaaaacaa tggcttcttt tctatcccca agagggtaat
     6241 gtcatcggtg tctataatcg gtatgagaat cttcttggca ttgcaggagg ctatggccgt
     6301 tgcaatgctt ggagttatct cccccaacat ggagttggga ataattattc ccagggaacc
     6361 aactatgaca tctaccttgt ttacggcaaa agttatagcg ctctctccgg tagcaccctt
     6421 gttggccttt tccttcatca tgttggaggt tgcggctgaa ttcgtcccca gggcatagat
     6481 tttaatgtca ctggggagat cctgcctcaa aagtgatatg atttgactgc cgatgcctcc
     6541 tcccatgccg tctactactg ctatattcat tttgatttct ctctctcaag tattatttta
     6601 tgttcaagaa gtttaatttc tttaatcacg gcatctacaa tcttctgttc acctaatagg
     6661 tcatacataa acaatttccc atcttgaggt tctatgtgga ttacattgtc catcagcttt
     6721 ttttcctggc catcttccag gatgtacacc ttagattcac acattctagc acctccctta
     6781 cttatatgtg ttttttggct tcaagcaagt aatcactggc ggctttaagg gagtcagctg
     6841 cttttgtaat catttcatat accttggcat ttccaatttt ctctgctttc tcagcccatt
     6901 ctctgaaacc ctgttcgtgg gagttgttat gctcaatcca gtgggctaaa agtattctca
     6961 aggtctcaac atctttttcc ttacttgccc tgcctccgca tcctctgcag caattgtgtt
     7021 cgctcatgat actcctcctt ctattttatg atatcacata aacaccctgt gtaaagctaa
     7081 tttcgtctag atgacagaaa caatcatccc ttaattgtgt ggttccttag tactgtcgtt
     7141 ataatcgcaa cttgaatact tgtggctgta aaacctataa attcatcgcc ttttttaagc
     7201 tggggaatag actggtatcc gtatgaggca gaaaacaggc gctaataaat tcttccatgt
     7261 aagccgggtc tgcgctcagt tcgatgtaag tcatactgtc ggcaatctcc tttacctttt
     7321 cccgagcatc gtcacttaca agacatgcat atgccccggt gagggagctg ttgccgataa
     7381 actcaaattt ttcatggggt aaatcgggca acaggccgat agtaattgaa ttgtttatat
     7441 caaggttttg ccctatgcct ccagcgatga tgaccctgtc aatatcggaa acatccattc
     7501 ctatgttgtt cagaagggtt tttgcaccgg aatagacggc acctttggcc cggaggaaat
     7561 tgtcgatatc aatctcattt attgttatgt ctagaccgtc cttggtttca ctgccgaagg
     7621 ctactacgta ttcaaaacag ccgaaatgct ggttgtacct tattctgtca gattttatgt
     7681 tttgatttat tttccccttc atatctatta taccctttaa aaacatttcg gcaataaggt
     7741 caattattcc agagccgcat atgcctttgg gttttgtgtc tccgatgacc ctgacaacgg
     7801 gtcttaaatt ttgcctatca attcttacct catcaatggc accgctggct gccctcatgc
     7861 cgcagctgat ttcgccgccc tcaaaggccg gtcctgccga acaagcacag gtcaacatcc
     7921 attccttatt tcccagcact atttctccgt tggtgcccaa atctataaag agcacggtct
     7981 cgtcaccgtt ccacagaccc gatgccaaaa ctcccgcaac tatatcgccg cccacatagc
     8041 ttgccacatt ggggagaata aataccgggg catttttgtt tataccaagt gaaatgtcgc
     8101 cagccttcaa tgcaggtgcg tttctaaatg ccggtatata aggttccagc cggatatttt
     8161 ccgcataaac cctcagcagc agatgggcca tggttgtatt acctgcaaag accgagaaaa
     8221 ctatttcgtc ttgtgtaatt tcaagctctt ttatcatttc cctgagcagc tgatttacgg
     8281 tgccttcaac tatggcatgg ttgagtttct ccagtccatt ttccttcgtg gaatagataa
     8341 tgcggttaat tatgtcgcca ccgtactgca tctgaaggtt accgctgttg gcagatgatt
     8401 ttatttctcc ggtctcaata tccaccaagc aggctgccac agtagttgtg cctacatcaa
     8461 cacaaagtcc ataagaagag gtcttgcttt cggcagacac attaatgaca tcgtatccgc
     8521 ctccattttt caaaagtgtc agggttattc cgtaatcgga atcccttaac agcacaggca
     8581 gctttctcag tgcatctatg gtccaattta ccattgtaca tcccaaaacg cttttcagct
     8641 ctcgctgtac cctttccagg tcggggatat tgtcatctat tgtaggctcg gggagcttga
     8701 cagggacaat cttaaaacta gttccaactt taatacctga atcctttaga atatcaatag
     8761 ccctgttgac taattttgtg cttgctctag acgttatatt ttccaccatt atgtcgttga
     8821 caacatccac ttccataagc ttaacggtca tgtcttcttg gacccaggta agacaggcaa
     8881 gcctcgtgcc ttgctggatt tcgtcttccg tgagaatggg agtgtattca tgcttgtgtt
     8941 ggcccgattt gaccgttacc cggcatttgc cacaatggcc tttgcctccg caaggggcgt
     9001 caagaaatac accggccttt cgtgccgctt ccattaaatt ggtgcccttg gcaacctcaa
     9061 cagccttacc tatgggcaaa aaggtaacta tacatttatc catattggtt atcaccctat
     9121 ctgtttcttc gaaatataca atttaaagct cccattttat taatactaca agccacttgt
     9181 taaaagtggc ttgtagggac tattattcca gggttttttc gtagtcactg ctagaaagga
     9241 gatttgcctc ttcagcagga tcgcttactt tcaccttaat tatccaaccg tcttcataag
     9301 gtgaactgtt taccagctca ggctcatcat cgagtgcttc atttacttcg ataatctctc
     9361 cgctaatggg tgcgtaaagg tctgaggcaa ctttagccga ttctatgctg ccaaagggct
     9421 caccctgagt gactgtatcg ccaacttcag gcaaatccac aaacaaaact tcgcccagct
     9481 ggtcttgggc atagtcagtt attccgactg tagcaacatc gccttcaact ttcgcccatg
     9541 tatgctcttg atgatatttc aaatctcctg gaaattccat atgaaaactc cttccctttt
     9601 ttagaacatt ggatccatgg ttagtgcagg atgacccaca ttggtcaaga attccacaac
     9661 tgtatccggg tccgttccaa cactttcatc tgcaatcatt tcatagaagt tttcgattcc
     9721 tatttctttg cctctctcta tcaatttttc tttaagagct tcttttaatt ctttaggcat
     9781 ccagactacc cgctgcaagc caccatcagc atataagaac tttttgctgg tgataaattg
     9841 tcttccgtgg cccataaatc caggtgtctg aactccgcca ccggtggttg atgccaattc
     9901 gccaaaggtc attccgagag gtgtcatgcc atggtattca cggttgacga taattacgcc
     9961 attagcttcc ggcatgattc cacagatgca ttcgaaacag ccgcaggaag tcatgggatc
    10021 ttccataatg gtataaatgt ttaccgatgt tgtagcacca ttggactgtt ccgttactgc
    10081 cttgtctaca gatgcccaat gaccttttct ttcatcaagg cattcgcctt taactatggg
    10141 ctgacaaggt cctgtgggat ctagttcgtg tgtagctttt gcgtcaagcc agcttacagc
    10201 accgcaaagg cccaagcgct ccggtgttac gatacacacg tgagcgggtg caaaggattg
    10261 acacagcagg caggagtaga aagtatccac actttcgtct gtaaggccag caagtcttgc
    10321 atctcgtgcg ttgtaccttg ggcgtgcgac ttcattgagc aattcttcta ccttttcttt
    10381 gtcagtgata attgtgactt gcaccttatc aacgatgctg ccaaactcat ctaacatctt
    10441 ggcatacata acttcaccga tatgtttcag cctaaatcct gcttcatagg aggatttgct
    10501 gattctcacc caggcgatat ctctctggcc gatatgcata acaccttcga tatagttcat
    10561 gaagtagtgg attctgcgtt ccattaccgg ttcaaagtct tcctgcatgt tcttacccgc
    10621 tacctcaact agtacggcca ggggcagttt gccaccttcg ggtccaagtg tatctacgtc
    10681 aggaccgatg agttcaatct tatgatcttc tacttccgac atttcacgca ttttgactaa
    10741 ttcccatgct gttgtacggc cgccaccaaa ttctgcaaac atatcgtcct tgcgcaccct
    10801 ttcaccttcg aaagcaggtg caaatcccac gggaattgga atctcggtaa cttttatctt
    10861 aatgccgcga gcctcaaggg atgattgaac caatttgtca taatcctttt gaacgatcag
    10921 gttcatggga acttcctgta catcctggtc agtaagtacg gggaagccca aggcaatagc
    10981 accggctcct gctgacacca caagttcgct taggggtccc aatgcattca cgaaggcagg
    11041 aaccctttcc ttggtgtatt ttaacagctc cgctaaatct ccgggcgtta catttccgaa
    11101 aataagggct gctcttattg caaatgatac aatgtggatt accgaagtca cgtcgtatcc
    11161 caggggaact actctaaagt caaggcctat cttaacacct gcttcgatgg cctggtcgat
    11221 aaccttgcca accaaggtca ccaggatacc cttggattga taatccttga taatctttgc
    11281 tgcctcttct gcagtaggtg cttcaccgat cacaacagct acaccgggaa tatcgccggt
    11341 tactaggggc acaccca
//
LOCUS       DAONUO010000014        11332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4419_length_11332_cov_4.266117, whole genome shotgun sequence.
ACCESSION   DAONUO010000014 DAONUO010000000
VERSION     DAONUO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11332
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4419_length_11332_cov_4.266117"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK768_01040"
     CDS             <1..274
                     /locus_tag="PK768_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59588.1"
                     /translation="TGLVGWPYLILYVLIPALLAICYRMKLPRIIGWQKARVALPFVL
                     LYIIDFAVIKGISTKSMATLLAVTVWPAFGEEFLYRGVLQQACSAW"
     gene            434..760
                     /locus_tag="PK768_01045"
     CDS             434..760
                     /locus_tag="PK768_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59589.1"
                     /translation="MYTLLVWASLFFSAFFDNIPYIATMLPVTSGIASLMGIDPTILY
                     FGLLSVATLGGNITPIGASANITGLGILKREGHEVSAKDFMKISVPSWRHCNDNIRIP
                     GFIKQF"
     gene            836..1423
                     /locus_tag="PK768_01050"
     CDS             836..1423
                     /locus_tag="PK768_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA59590.1"
                     /translation="MNQYVTGAIIKRLREERKMTQAELAQVLNVSDKAVSKWETGKGY
                     PDITLVEPIAKALGISTIELLAGESISNRNRSFNMLRSKFYVCPVCGNVMAATGESVI
                     SCCGVKLPPLEAEEPDEGHQLEIETVEDEYYVSLNHEMTKEHYISFIAGVSDIGIQLV
                     KLYPEGGAEARLKINRTRVFYYYCNHHGLFKITRR"
     gene            1582..1776
                     /locus_tag="PK768_01055"
     CDS             1582..1776
                     /locus_tag="PK768_01055"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HQA59591.1"
                     /translation="MTTCSNTITCTCTYSSCSRRGKCCECVAYHRRSGEVPGCFFSAA
                     GERTYDRSIENLYRDYKGNR"
     gene            2093..2590
                     /locus_tag="PK768_01060"
     CDS             2093..2590
                     /locus_tag="PK768_01060"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59592.1"
                     /translation="MCTSFQGKSTPWCLRCEVGNTVCGRTAGGVCPRCPGAHLSIGYH
                     KKDAGYFRSRSLPPFRSNIHYPEEIIGGRKAALKIVFVTPEEFGFSRDMMKENRIPLI
                     VCSRVGLKGLVMHTEMAHIFRQAKDGFFMFSRFWLGERLKSGIIKKDGHDGIPGQGFG
                     GALLH"
     gene            2699..2842
                     /locus_tag="PK768_01065"
     CDS             2699..2842
                     /locus_tag="PK768_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59593.1"
                     /translation="MKNYLVEHTEHLGTETTNEILANMKKSIENPDEFLADKGVHRTV
                     FGL"
     gene            2832..3020
                     /locus_tag="PK768_01070"
     CDS             2832..3020
                     /locus_tag="PK768_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59594.1"
                     /translation="MAFKQSDEYKNSPMFQIESILKEFCSTSGYNDVFITALKELSPS
                     YADYCKQLEIANNKLMTE"
     gene            3322..3579
                     /locus_tag="PK768_01075"
     CDS             3322..3579
                     /locus_tag="PK768_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA59595.1"
                     /translation="MARRCLSGRRSKKTGAHRGDRPWEGLKQSALPHIWVRYFKVDKK
                     NKRPLIGTGLGLSIVKKVVEMHAGRYGVESEEGVGSIFCLK"
     gene            3672..5411
                     /locus_tag="PK768_01080"
     CDS             3672..5411
                     /locus_tag="PK768_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA59596.1"
                     /translation="MRRYWKYVKPYLSAFILGPIFMIVEVIGEVVMPLLLSNIIDYGI
                     NGGRGIAYITAMGITMVITALFMMAGGVGGAYFAIKASTGFANDLRKDLFNQIQKFSF
                     NNIDQFNTGSLITRLTNDVTQVQNMIQMLLRMALRSPGMLIGALIMAFVLNPRLALVI
                     LCVIPLLACAIYFIMKVAFPLFTAMQKKLDALNTATQENLTNIRVVKSFVRERHEEEK
                     FKKANTDLKDSTIKAMNVVIFTLPVMTLAMNITTLAVVWFGGKQVIAGSMTPGVLTAF
                     INYIVQILISLMIVSFIILISSRTLASIKRIDEILDTEIDLTDEEARHKDRAVQHGKI
                     EFRGVYFKYYKNNEKWVLEDVNLVINPGETVGIIGSTGSGKSTLVQLIPRLYDVDRGE
                     VLVDDVNVKDYALKNLRDGVGIVLQQNVLFSGTIKENLQWGDEEARDDEIYQSAERAQ
                     AHGFITSFEAGYDTELGQGGVNVSGGQKQRLCIARTLLKKPKILILDDSTSAVDTATE
                     AKIRESLRNELKGTTKIIIAQRISSVIDADRIIVLDQGRIVGMGTHAELIQSCEAYSE
                     IYYSQMDKKVAAS"
     gene            5408..7333
                     /locus_tag="PK768_01085"
     CDS             5408..7333
                     /locus_tag="PK768_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA59597.1"
                     /translation="MSRLSAERKETLLRRYGSQGINAGAGPGGRRPGGFGGPGASRVG
                     MKGRPKNISATISRLLSYIGQDKVKILFVFACVLGGNLSSLGGSYILRPVINNLVYPG
                     LSAEEKIKNLVIGILVMAGIYLVGAVCIYLQRKIMIGVSQKALVKIREELFHKIQKLP
                     LKYHDTHAHGDIMSRFTNDLDSVGEMLNHTMVQVFSAVLTLLGTVILMFVTNWLLAIV
                     TLTTVPLLVYAGGIIGKQSRKYFTGQQQAIGAVNGYIEETIAGQKVIKVFSHEETAME
                     EFAFLSDELQKKQVRAQFFGGIMGPVIGNLSQISYAITATVGGILCLTANFDIGGLTI
                     FANYSRQFSMPINELSMQMNIIFAALAGAERVFEVMDETPEAADPEEAIEICSEANRA
                     EGARVVKGEVELKNVTFGYIPGKAVLKNINITAKPGQKVAFVGSTGAGKTTITNLINR
                     FYELEEGEILIDGINIKQIKKDSLRSNIAMVLQDTHLFSGTVRENIRYGRLDATDEEV
                     VAAAKISCAHSFIERLPQGYDTLLDGDGANLSQGERQLLNIARAAISKAPILILDEAT
                     SSVDTRTEKYIERGMDQLMKDRTTFVIAHRLSTVRNADKIIVLENGEIIEQGTHEELL
                     EKGGRYYQLYTGAIELD"
     gene            complement(7339..8511)
                     /locus_tag="PK768_01090"
     CDS             complement(7339..8511)
                     /locus_tag="PK768_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQA59598.1"
                     /translation="MQLNTLFYLALILFSGLIFGRLGKLLKLPNVTGYLIAGLLIGPC
                     LFKLVPLNVTSQLTIVSEMALAFIAFSIGAEFKIPYLKKVGATPIVIAVFEALAASIV
                     VTVVLLLFKFDIKIALVLGAIASATAPAATIMVIKQFAAKGPVTETLLSVVALDDAVA
                     LIAFGFAAAIAKSLQHPHEVSVIMSMVSPVIEIVGSILLGFLLGILFTIPLRYFKKDS
                     NRLIITTAFVFLGSSLATMLGLSPLLLCMCMGAMLINISQAGGAILKLVDIVTPPIFL
                     MFFVISGMELNIYVIPQIGVIGIIYVICRVVGKVAGSYLGAAMMKAPAPVKKYLGFTL
                     IPQAGVAIGLSLVAAGDFPELGETIRTVVLCATLIYELVGPAVTKFSLEKAGEIRG"
     gene            complement(8515..8853)
                     /locus_tag="PK768_01095"
     CDS             complement(8515..8853)
                     /locus_tag="PK768_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59599.1"
                     /translation="MKLLLIVLNKVELLDTLLERFMDNGIHGATILNSTGMARELAKS
                     NHDLPIFGNLRYIIDPEREESKTIFIVLKDEQVEEAKKIVRDVVGDISKPDTAVIFTL
                     PVLSAEGVDF"
     gene            complement(8928..9089)
                     /locus_tag="PK768_01100"
     CDS             complement(8928..9089)
                     /locus_tag="PK768_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59600.1"
                     /translation="MQKSDTVDSIVTFIDEILCYDENMGSRWNPLNLPWKFWQKYREA
                     RDAPFLHPS"
     gene            complement(9198..9677)
                     /locus_tag="PK768_01105"
     CDS             complement(9198..9677)
                     /locus_tag="PK768_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQA59601.1"
                     /translation="MPSSIKKSAEKVQAALDKFGLELKVIELPDSTRTAQEAAKAIGC
                     TVGQIAKSLIFKGKTSQNPILIIASGPNRVNENAIKEYAGEELVKADARFVLEQTGFA
                     IGGIPPVGHKTPIITFIDEDLMLYDEIWAAAGTPNAVFKLTPEILVDITKGRVIKVK"
     gene            9883..10377
                     /locus_tag="PK768_01110"
     CDS             9883..10377
                     /locus_tag="PK768_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQA59602.1"
                     /translation="MSENKTLANLMEAFAGESQANKKYLAYAKKAEKEGKLNAAKLFR
                     AAADAEKAHALRQLEVAGKVGSTADNLKSAVEGETYEFTDMYPQFLKDAEAEGNKAAV
                     TAFTFAMKAEEVHARLYKEALENLDQTEEVFYYLCPICGNIEKSIPEKCSICGFPGEK
                     FIKY"
     gene            complement(10495..>11332)
                     /locus_tag="PK768_01115"
     CDS             complement(10495..>11332)
                     /locus_tag="PK768_01115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQA59603.1"
                     /translation="ITPEVTLRGIKSVRQGKAIPCSRPIQLIDPSDPNHSPASHKMYP
                     MLDAGLFNGSFDELTFYTHSMLNTHIDAFCHVGIAGYAFNGRKFDETVNMEKGAVYCD
                     ITDMLSIVTRGVFIDVARNRGVDGLRPGEYVTPDDLKPLLERLQPGDAAVVRTGVTLI
                     GGNVDERDEEGKPKLHYGIAGFHADCIPLLAEKQISVLASDSPSDTYPCPVEGLDGPH
                     DSPVHGLCLTLYGIPLCHNMDLEEVGRISEETGQQEFLFMVAALHLKHGTGSPCTPLA
                     VF"
BASE COUNT         3073 a         2532 c         2869 g         2858 t
ORIGIN      
        1 aacaggactt gtcgggtggc cgtatttgat attgtatgtg ctgattccag cgcttcttgc
       61 catctgctac cgcatgaagt taccccggat aataggttgg caaaaggcac gcgtcgcact
      121 tccattcgtt ctgctttata taattgattt tgctgttata aagggcatat ccaccaaaag
      181 catggctaca ttgcttgcag ttacggtgtg gcccgctttt ggcgaagaat ttttgtatcg
      241 gggtgtactt caacaagcct gctcggcatg gtaaaaaagc ctgtcacagc catcgtcctt
      301 acatccatcc tgtttgcggc aagccatatc ccgatttatg tgttggctgc gtccggcgaa
      361 ggttttacca accttctcaa aagaggcttt acagatacat ttcgttatct ccatggcgat
      421 gtgaaaggcg tttatgtata ctcttcttgt ttgggcctcg ctgtttttct ccgcgttttt
      481 tgacaacatc ccttatattg caactatgct gcccgttacg agtggaattg caagccttat
      541 ggggatagat cctactattc tctactttgg tcttctctcc gtggcgacgc ttggaggtaa
      601 tatcacgcct atcggagcgt ccgcaaatat cactggcctt ggcattttga agagggaagg
      661 gcacgaagtc agcgcgaagg attttatgaa aatcagtgtg ccttcatggc ggcattgtaa
      721 cgacaatata cggattcctg gctttatcaa acagttttga tgctcatctc tacttttcgt
      781 aggtaggttt cgtctgtaac atatgctatt cttaatacgc aaggaggagg ttttcttgaa
      841 tcaatatgta actggagcaa ttattaagag gcttcgcgaa gaaagaaaaa tgacccaagc
      901 agagcttgca caggttctga atgtgagcga taaagctgta tcgaaatggg aaacaggaaa
      961 aggatatcct gatattacgc ttgtcgaacc catcgctaag gctcttggca tatcaacaat
     1021 tgagctgttg gcgggtgaaa gcatatcaaa ccgtaaccgt tcatttaata tgctgagaag
     1081 caagttctat gtgtgtcctg tatgtgggaa tgttatggct gcgacaggcg aaagcgttat
     1141 tagctgctgc ggtgttaaat tacctccgtt ggaggctgaa gaacctgacg aaggccatca
     1201 acttgaaata gaaacggttg aggatgagta ttatgtcagc ttaaatcacg agatgacaaa
     1261 agagcattat atatccttta ttgcaggtgt gtccgacatt ggtatccagc ttgtgaagct
     1321 ctacccggaa ggcggtgcag aggcaaggtt aaaaataaac aggacaagag tgttctatta
     1381 ctactgcaac catcacggat tgtttaaaat aacaaggcgg tgacatcgat attatttgct
     1441 caactgtacc ctgggggatg cttaggccaa gattgttcgc ccgattttag tttcctggca
     1501 gttcgccgga cctgctttat tgaaaggcaa tttgtttttt actataatga ttatgtcaat
     1561 ttatattttg ggggatttga aatgactact tgcagcaata ccatcacttg cacctgtaca
     1621 tatagctcgt gttctcgacg cggcaaatgc tgcgaatgcg ttgcctacca ccgcagaagc
     1681 ggagaggtgc ccggatgctt cttttccgcg gccggtgaaa ggacttatga tcgctccatt
     1741 gagaacctct atagggatta taaagggaac aggtaaagat gcaggccggc gaatggctca
     1801 gcgccagggc ccgttgatat tgcttattaa aagttgtttt cggtcttgca gagattaaac
     1861 tgcccggaat ttattaaact tttttatcga agtttttatt tgctttattt ctagaaaccc
     1921 ttgacaacat cagcacccat tcctcgcgga gcgggacgcc tcgttgcatt tcttcagcaa
     1981 agccggtaaa atccatgcct gcactgttgc cggcggcagc agcaccacat taagcgctct
     2041 gcatgagatt gtaggctaaa ccgcatttta tcacagctta tctcaagacc aactgtgcac
     2101 aagcttccag ggcaaatcta ccccctggtg tctcaggtgc gaagtaggaa acactgtatg
     2161 cggccgaaca gccggcggcg tttgcccccg gtgtcccgga gcccacctgt caatcggcta
     2221 tcataagaag gatgccggct acttcaggag caggagcctg ccgccattcc gcagcaatat
     2281 acattacccg gaggagatca tcggagggag aaaggctgcg ctgaagattg tttttgtgac
     2341 cccggaagaa ttcggttttt caagggatat gatgaaggaa aaccggattc ccctgatcgt
     2401 ctgcagccgc gtgggcctca agggtttggt gatgcatacg gaaatggccc acatttttag
     2461 gcaggcaaag gatggttttt ttatgttcag ccgcttctgg ctgggggaga gattgaaaag
     2521 cgggattatc aaaaaagatg gccatgacgg aatccctggc caggggtttg gcggagcatt
     2581 gctgcattga gtacaaaaat ctcgccatga gcccattgtg acggaaaaac aaagagcggc
     2641 atacgagaaa atcagatcat ttttggataa ctgccccccg cttgatcttc cggaagactt
     2701 gaagaattat ctagtagaac atactgagca tctgggcacg gagacgacaa atgaaatact
     2761 ggccaacatg aagaaatcca tagaaaaccc tgacgaattt ctggcggata aaggagttca
     2821 tagaacagta tttggccttt aagcagtcag atgaatacaa gaactccccc atgtttcaaa
     2881 tagaatcgat cttaaaagag ttttgcagta caagcggtta taacgacgtg tttatcaccg
     2941 cattaaaaga actgagcccc tcctacgccg attattgcaa gcagctggag atagccaata
     3001 acaagttgat gacagagtag ggcctgcttg ccgaagaggg cgcgatccgg cggcggaagt
     3061 ccgcttcctc cttaagtgcc agggcaactc ttcttttttt cgctcatttc gcctcaacct
     3121 ggtggggcca aggttgctat tctcccccaa ccaaaatctt cagctaaagt ttagacagca
     3181 aacaattggg cttcagtttt ccggcataac attttgtgat cgagttattt catcaagcca
     3241 tcccctttgt ctttgccgac gaagtaaaga ttacccagtt attttataat ctcttgctca
     3301 acgccatcac ccacagcggc catggcaagg cggtgcttgt ccggcagacg gtcaaagaag
     3361 accggtgcgc atcgaggtga tcgaccatgg gagggcctca agcaaagcgc tctgccccat
     3421 atctgggtgc gttatttcaa ggttgacaaa aaaaataaaa ggcctttaat aggtacaggg
     3481 ttgggacttt caattgtaaa aaaagttgtt gaaatgcacg ccggccggta cggcgttgaa
     3541 tccgaggaag gggtaggcag catcttttgt ttgaaataga attggggagg atgcatcaat
     3601 aaaaaattga tgccggagaa aacggctcaa caaaattgcc ccatataatt taaagctaag
     3661 ggaccattgc catgagacgc tattggaaat atgtaaagcc atacttgtct gcatttatcc
     3721 tagggccgat tttcatgatt gtagaggtta tcggtgaagt agttatgcct ctgttattga
     3781 gcaatattat cgactacggg attaatggcg gcaggggcat tgcttatatc acagccatgg
     3841 gcataaccat ggttatcacc gcactgttta tgatggccgg cggtgtagga ggagcttatt
     3901 ttgccattaa agcctccacg gggtttgcca acgatttgcg aaaagacttg ttcaatcaaa
     3961 ttcagaagtt ttcatttaac aacattgatc aatttaatac cggatctttg atcacccggt
     4021 taacaaatga tgttacccag gtacaaaaca tgatccaaat gttgttaagg atggctttaa
     4081 ggtcgcccgg catgcttatc ggtgcgctca taatggcttt tgtgctaaat ccgaggctgg
     4141 ccctggtaat cctttgtgta atacctttgc tggcttgcgc catctatttc attatgaaag
     4201 tagcctttcc cctgttcact gccatgcaga agaagctgga tgcgctaaat accgccactc
     4261 aggaaaattt gaccaatatc agggtggtaa aatcctttgt aagagaacgg catgaagaag
     4321 agaaatttaa aaaagccaat actgacctga aggacagcac catcaaagca atgaatgtgg
     4381 ttatctttac cctgcccgtt atgactttgg ccatgaacat caccactttg gccgtggtct
     4441 ggtttggcgg aaaacaggtt attgccggca gcatgactcc cggcgttctg actgccttta
     4501 tcaattacat tgtccagatc ctcatctcgc tgatgatcgt ttccttcatc attctgatca
     4561 gttccagaac cctggcctcc ataaaacgca tcgatgaaat tcttgataca gagatcgacc
     4621 tgaccgacga ggaagcccgg cataaggata gggctgttca acacggcaaa atcgagttta
     4681 ggggagttta ctttaaatac tataaaaaca atgaaaagtg ggttctggaa gatgtcaacc
     4741 tggtaataaa tcccggcgag acggtgggca tcatcggttc caccggcagc ggcaagtcaa
     4801 ccctcgtgca gctgatcccc aggctttatg acgtagaccg gggagaagtg ctggttgatg
     4861 acgtgaatgt gaaagactat gccctaaaga acctgcgcga cggcgtcggt attgtcctcc
     4921 agcaaaatgt actgttttcc ggtaccatta aagaaaatct ccagtggggc gatgaagagg
     4981 cgagggatga tgagatatac caatctgcag aaagggcgca agcccatgga tttattactt
     5041 cctttgaggc cggctatgat accgaactgg ggcagggcgg tgtcaatgtc tccggcgggc
     5101 agaagcaaag actgtgcatt gcccgaacgc ttttgaaaaa gccgaaaatt ttgattctcg
     5161 atgacagcac cagtgccgtg gacacggcca cggaggcaaa gattagggaa agcttaagga
     5221 acgagctgaa agggacaacg aagattatta tcgcccaaag gatttcttca gtaatcgatg
     5281 cggatcggat tatcgtcctt gaccagggca ggattgtggg catgggcacg catgcggaat
     5341 tgattcaaag ctgtgaagcc tattcggaga tttattactc ccagatggat aagaaggtgg
     5401 ccgcatcatg agtcgcctca gcgcagaaag aaaagaaacc ttattgcgca gatatggtag
     5461 tcaagggata aatgccggcg ccggccccgg gggtagaagg ccgggcggct tcggggggcc
     5521 tggagcctcg cgcgtgggca tgaaaggcag gcccaaaaac atctcggcga ccatcagcag
     5581 gctgctgtcc tatatcgggc aggacaaggt taaaatcctc tttgtttttg cctgtgtcct
     5641 gggcggcaac ctgtccagcc tgggcgggag ctatattttg cggccggtca ttaataatct
     5701 ggtctatcca gggctgtctg cggaagaaaa gatcaagaac cttgtcatag gcatcctggt
     5761 tatggcgggc atctatctgg tgggtgccgt ttgtatctat ctgcagcgga aaatcatgat
     5821 cggagtctcc caaaaagccc tggtgaaaat cagggaagaa ctctttcaca agattcaaaa
     5881 gcttccgttg aaatatcacg acacccatgc ccacggcgat atcatgagcc gttttaccaa
     5941 tgacctggat tccgttggcg aaatgctcaa tcataccatg gtgcaggttt tctccgcagt
     6001 gcttactttg ctgggcaccg tcatcctgat gttcgtcacc aactggctgc tggcaattgt
     6061 cactctgact acggtacctt tgctggtcta tgcgggaggc ataattggaa agcaaagcag
     6121 gaaatatttc accggccagc agcaggcgat tggcgccgtt aatggctata tcgaggaaac
     6181 tattgccggc cagaaggtta taaaagtatt ttcccacgaa gagacagcca tggaagaatt
     6241 cgccttcctg agcgatgaac tgcaaaagaa gcaagtaaga gcccagtttt ttggcggcat
     6301 tatgggaccg gtcattggca accttagcca gatcagctat gccataacgg cgacggtggg
     6361 cggcatcctg tgtttgaccg ccaattttga tatcggcggc ctgaccatct ttgccaacta
     6421 ttcaaggcag ttttccatgc ccatcaacga gttgtccatg cagatgaaca taatcttcgc
     6481 cgcattggca ggggcggaaa gagtgttcga ggtaatggat gagacgccgg aagcggcaga
     6541 cccggaggag gccatcgaga tttgctctga agccaaccgg gcggaagggg cacgggtggt
     6601 aaaaggggag gtggaactga agaatgtgac ctttgggtat attcccggca aggccgtact
     6661 gaaaaatatc aacatcacgg caaaacccgg ccagaaagtg gcttttgtcg gctctaccgg
     6721 tgccgggaag accaccatta ccaacctgat caacaggttt tatgaactag aagagggaga
     6781 gatattaata gacggcatca atataaagca aattaaaaag gattcattaa gaagcaatat
     6841 agccatggtg cttcaggata cccacttgtt ttcgggtacg gtcagggaga atattcggta
     6901 tgggcgtctt gatgccaccg atgaagaagt agtcgctgcg gccaagattt catgcgcgca
     6961 ttcctttatc gagaggctgc cccagggata tgataccttg ttggacggcg acggtgccaa
     7021 cttgagccag ggcgagcgcc agcttctcaa tatcgcccgg gccgccatat caaaagctcc
     7081 tatactgatc ctcgacgagg ctaccagttc cgtggatacg agaacggaaa agtatattga
     7141 acggggcatg gatcagctca tgaaggacag gacgaccttt gttatcgccc atagactgtc
     7201 cacggttcga aatgccgaca agattatagt attggaaaat ggggagatta ttgagcaggg
     7261 cacccacgaa gaacttctcg aaaagggcgg gagatattac cagctataca ccggagcgat
     7321 tgagctggac taacaaactt atcccctaat ttctcctgct ttctccaagg aaaatttggt
     7381 aacggcaggg cccaccagtt cataaatcaa agtggcgcaa agaactaccg tgcggattgt
     7441 ttctccaagt tcgggaaagt cgccggcggc aaccagcgaa agaccgatgg ccactcccgc
     7501 ctgcggaata agtgtaaatc ccaggtattt tttaacgggg gccggcgcct tcatcattgc
     7561 agcacccaga tacgatccgg ctactttgcc gaccacgcgg cagattacat aaataatgcc
     7621 gataaccccg atttgcggta tgacgtaaat gtttagttcc atgcccgaaa tgacaaagaa
     7681 cattaggaaa atggggggcg ttacaatatc gacgagttta agaatggcgc cgcctgcctg
     7741 gcttatgttg atcagcatgg cccccatgca catgcaaagc agcaatggag acaagcccag
     7801 catggtagcc agggaggaac caaggaatac aaaggcggtg gttataatta agcggttgga
     7861 atccttcttg aaatagcgca gcggtattgt aaacagtatg ccgagcaaaa agcccagcag
     7921 aatagagccg acaatttcaa taacgggcga aaccatcgac attattacgg agacctcatg
     7981 gggatgctgc aggctttttg caatagctgc tgcaaagccg aaggcaataa gggcaactgc
     8041 atcatccagg gcgacgacac taagaagggt ttcggtaacg gggccttttg ccgcaaactg
     8101 ctttattacc atgatcgtgg cggcgggtgc cgtggccgaa gcaatggcgc cgaggactaa
     8161 agcgattttg atgtcgaact taaataaaag caataccaca gttacgacaa tagatgccgc
     8221 cagcgcctca aagacggcga tgactatagg agtagctcca acctttttca agtaaggtat
     8281 tttaaattcg gcgcctattg agaaagcaat aaaggccagg gccatttcgg aaacaattgt
     8341 tagctggctg gttacattaa gaggaaccag cttgaagagg cacggtccta tgagcaggcc
     8401 ggcgatgagg tagcctgtca cattggggag cttaaggagt tttccaaggc ggccgaagat
     8461 aagtccagaa aataaaataa gggccaggta gaaaagtgta tttagctgca ttttttagaa
     8521 atcaacccct tctgccgaga gaacgggcag ggtaaagatg acggcagtat ccggctttga
     8581 tatgtctcct acaacatccc gaacaatctt tttggcttct tcaacctgct cgtcttttag
     8641 aacaataaat atggttttgc tttcctccct ttccggatcg ataatatagc ggagattgcc
     8701 aaatatgggc aaatcatggt ttgattttgc aagctccctg gccatgcctg tgctgttcag
     8761 gatagtggcg ccgtggatgc cgttatccat gaacctttcc agcaaggtat ccagcaattc
     8821 gaccttgttt agcacgatca gcagcaactt catacatcat cactccagtt aaattgatat
     8881 aaaaggctga ttaagcgaac attctcctcc cggattcttc ccggaagtta tgaagggtgc
     8941 aggaaggggg catccctggc ttcccggtat ttctgccaaa atttccaggg aaggttaagg
     9001 ggattccagc ggctgcccat attttcatca tagcataaga tttcatcgat aaaggtaact
     9061 attgaatcga cggtatcgga tttttgcaaa aggttgaccc ggtagcagcg gcgtgaatgt
     9121 gcttacagtc atggaaataa gtaaaaaatg gagaggccaa tagaaaataa acgttaaata
     9181 aggggaaacc ggcgttttta ttttacttta ataacccttc cttttgttat atccaccaga
     9241 atttccggag taagtttaaa aaccgcattg ggggttccgg cggcggccca tatttcatca
     9301 taaagcataa gatcttcgtc aataaaagtg atgattgggg ttttatgccc caccggcgga
     9361 attccgccga tggcgaagcc tgtttgttcc aagacaaatc tcgcgtctgc ctttaccagc
     9421 tcttcgccgg catattcttt aattgcattt tcatttaccc tatttggtcc actggcaata
     9481 attaatatag gattctgcga ggttttgcct ttaaaaatca gcgatttggc tatctggcca
     9541 acggtgcagc ctatagcttt tgcggcttct tgggcggtac gggtagaatc gggcaattct
     9601 attaccttaa gttcaagccc aaatttatca agtgcagcct gcactttttc tgcgcttttt
     9661 tttatactgg agggcataat aatctcctcc ttattgcagc tctgccgagg aagggggcat
     9721 ataaataata attataattg gaatttgaaa atccttgctg ccattatctt ccgaagaata
     9781 tttattcaaa cagataattt attgtataat ttaactaaag acatggcacc cacatcgttc
     9841 tatattgatt tcggattaca ataaagcaag gaggaaaaga cgatgagcga aaacaaaacc
     9901 ctggccaatc ttatggaagc gtttgccggg gaatctcagg ccaacaaaaa gtacctggct
     9961 tatgccaaga aagcggaaaa agaaggaaag cttaatgccg ccaaattgtt cagggccgct
    10021 gccgatgccg aaaaagccca tgccttgaga caacttgaag ttgccgggaa ggtcggctct
    10081 actgctgaca acctgaaaag cgctgtcgag ggcgaaacct atgaatttac ggatatgtat
    10141 ccccagtttt taaaggatgc tgaagccgag gggaacaaag ctgctgtcac ggcctttact
    10201 tttgccatga aagcggaaga ggtgcacgcc aggctgtata aggaagcgct ggaaaacctt
    10261 gaccagacgg aggaagtgtt ctattatctc tgcccgatat gcgggaacat cgaaaaatct
    10321 attccggaaa aatgcagcat ctgcggtttt ccgggggaga aattcattaa atactagcct
    10381 gggaattggg caataaaaaa atagccgtcc cagcttggga ataggacatt aaataaggca
    10441 ctcaacgttc agttgcgttg agtgccttat tgttttgctt agttgtgccg ccaattaaaa
    10501 aactgccaga ggagtacagg gagagcctgt accatgtttt aaatgcagtg cagcaaccat
    10561 aaatagaaat tcttgttgtc cggtttcttc gctgatgcgc cctacctctt ccaggtccat
    10621 gttgtggcac agaggaatgc cgtaaagggt taggcatagg ccgtgaacag gggagtcatg
    10681 agggccgtcc aagccctcca cggggcaagg gtacgtatcg ctgggagagt cagatgccaa
    10741 aacgcttatt tgtttttctg ccagcagggg aatgcaatcc gcatgaaagc cggctattcc
    10801 gtagtgaagc tttggcttcc cctcttcgtc gcgttcatca acattgccgc caataagggt
    10861 aacgcctgtg cgtactaccg cagcatcccc gggttgcaag cgctccaaca agggtttaag
    10921 gtcatccggg gtaacatatt cacccggcct gaggccgtca accccacggt tgcgggcgac
    10981 atcaataaat accccccggg ttactatgct gagcatatcc gtaatgtcgc agtatacagc
    11041 gcccttttcc atattgactg tttcatcaaa tttcctgcca ttaaacgcat atccggcgat
    11101 tcccacatgg caaaaagcat caatatgtgt gttcagcatg ctgtgggtgt aaaaggtgag
    11161 ttcatcaaac gaaccgttaa aaagaccggc atcgagcatg gggtacatct tgtgggaggc
    11221 ggggctgtgg ttgggatcgc ttgggtcgat caactgaatg gggcgggaac agggtattgc
    11281 tttgccttgc cgcacggatt ttatgccgcg cagggtcact tccggcgtaa tg
//
LOCUS       DAONUO010000015        11331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4420_length_11331_cov_4.087620, whole genome shotgun sequence.
ACCESSION   DAONUO010000015 DAONUO010000000
VERSION     DAONUO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4420_length_11331_cov_4.087620"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PK768_01120"
     CDS             complement(<1..834)
                     /locus_tag="PK768_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HQA59604.1"
                     /translation="MQSKVRKLRTFFNYGKKSKIKTGAEYINMLRVLMIGDIVGRPGR
                     TIIREKLLYIKDKYEVDYVIANGENAAGGNGITEKIAQELFISGIDFLTMGNHVWDNK
                     DVFNFIETENRMIRPANYPIAPGKGYQTVEIKKGPILGIINISGRTFMPPLDCPFRTA
                     DAIIEQISSITKIIIVDFHAEATSEKIAMGWYLDGRVSLVAGTHTHVQTADEKILPNG
                     TAYITDVGMTGPIDSVLGIKKESVINKFINQLPVRFEVAKGPAELNGIIVDIDELTGK
                     AL"
     gene            complement(824..2335)
                     /gene="rny"
                     /locus_tag="PK768_01125"
     CDS             complement(824..2335)
                     /gene="rny"
                     /locus_tag="PK768_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQA59605.1"
                     /translation="MIVGLIALAAGYLIRKYIAEAKIQSAEESAKKIIEDAENKAESI
                     KRESVIEAKEEILKLKNEAEKEQRERRNELQRTERRLIQKEEALDRKLESIEKKEEAI
                     SKKQNEINQLHEKINDLYSQQIAELERISALTTDEAKELLLNKVKDEIRHDAAIMIKE
                     IESQAKEEAQKRAREIISCAIQKCAADHVAETTVSVVTLPNDEMKGRIIGREGRNIRA
                     LETLTGIDLIIDDTPEAVILSGFDPIRRQVAKIALEKLILDGRIHPARIEEMVEKAQK
                     EVDNIIREEGEKATFDTGIHGLHSDLVKYLGKLYFRTSYGQNVLHHSIEVAHLAGAMA
                     AELGVDIKLTKRAGLLHDIGKSVDQDIEGPHALVGAELAKKYGESPEVVNAIASHHND
                     VEPQTIEAILVQAADAISAARPGARRETLEAYIKRLEKLEEIANSFAGVEKSYAIQAG
                     REIRIMVKPEAVDDLGVIEMARGISKKIEEELEYPGQIKVNVIRETRAIEYAK"
     gene            complement(2611..3279)
                     /locus_tag="PK768_01130"
     CDS             complement(2611..3279)
                     /locus_tag="PK768_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQA59606.1"
                     /translation="MTGEFDKSIDYIDKYTQQLIVEHAMKKSIKEKLEKDLTSKENEL
                     EEVTNSIDILEQVRLLLQRASEYAREQIKQQIEMLVTHCLQFVFGPNLEFEIELNEFR
                     GKSSAEFYVVSTYDDVKIRTKPQDARGGGIVDVISLALRIAVIQSTDMYKDGPIILDE
                     PAKHVSNEFINNVAQFLKQVSNVFHRQIIMVTHNQYLSEISDLAYKVELKNGISEVTV
                     LNSE"
     gene            complement(3282..3524)
                     /locus_tag="PK768_01135"
     CDS             complement(3282..3524)
                     /locus_tag="PK768_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59607.1"
                     /translation="MDQEKEIHELKQKIDKAKAMRYKAEVRLEELQKQRQSLLDELNK
                     LNVKPEELDKEIEKISNEINDLLKKAKDLLPKNIEG"
     gene            complement(3544..4986)
                     /locus_tag="PK768_01140"
     CDS             complement(3544..4986)
                     /locus_tag="PK768_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA59608.1"
                     /translation="MGSIKSLKLINFQSHKETQINFHEGLTVILGQTDQGKSAIIRAL
                     KWVLYNEPRGTDFITAGCKYCRVELEMQDGSKIIRERDGQRNRYILEQNGQEHIFEGF
                     GNTVPLEIVKAHGIPKIFIDRDATSAVNLAEQLEPPFLISESGSNRAKALGRLVGIHI
                     IDAAQRTTLKDLVDKQQRYKLLEEEIENIRDELQSYNDVDKLEKKISKLSGILEKLKQ
                     KRNILFKLTQLQQELEPTDIEIEKNKGILSKLDFIEEAEKNIRIIDALHSKYNYLCQL
                     MKKLALAADSIKVEQELVKATKSLDSIEVYYTSILKLAQKQERLINIRRNLIDNERNT
                     NGIKRVLKDTENAYFADILLLEAGKLLEALKRYISIRDKWRSVDYQLGVHTKEIAQYV
                     HMDKSEHYLTELSQKMKKLSLLQNIKDSILTVESSINKGEAYLEKLESNLALMAKEYS
                     QLLERLSICPTCLAPIDKETTQKIVSDLLN"
     gene            complement(4989..5984)
                     /locus_tag="PK768_01145"
     CDS             complement(4989..5984)
                     /locus_tag="PK768_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA59609.1"
                     /translation="MRILFFTDTHIRGNNPQNRKDNFAETLFQKLEEVFDIAKDNDVQ
                     LLLHGGDIFDRPDIAPSLVRDFILLVDKYSLPIYAVAGNHDVFGQNPLTLNRTMLGLL
                     DGAGLIKLINPGEKLYFKENNKKIQLTGQHYFYGIDADDEKRSYVVRKDPDVDFAIHV
                     VHGMLLEKPFFEGMAYTLIEDILDTEADITLSGHYHAGFATKCIDNKYFINPGSLVRI
                     NNSLNEFLRMPKVILLDLEDDVRIEEIYLNKALPGEDVLDRSKVESMAFRERKLSEFI
                     QSVYSTGNYDTFDINKIIEEISKQQNIKDEVIMEALKRIGQAQELLGKEDWLEVS"
     gene            complement(5986..7017)
                     /gene="recA"
                     /locus_tag="PK768_01150"
     CDS             complement(5986..7017)
                     /gene="recA"
                     /locus_tag="PK768_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQA59610.1"
                     /translation="MAEKDRALETAIAQIERQFGKGSIMRLGDAASKFNIEAIPTGAL
                     PLDLALGIGGVPRGRIIEIYGPESSGKTTVALHIVAEAQKMGGTAAFIDAEHALDPNY
                     AKNLGVDTDNLLISQPDTGEQALEIAEALVRSGAIDVVVIDSVAALVPKAEIDGEMGD
                     AHVGLQARLMSQALRKLAGAINKSKTVAIFINQLREKVGIMFGNPEVTPGGRALKFYS
                     SVRLEVRKSEILKQGDEMLGNRTKVKVVKNKVAPPFKQAEFDIIYGEGISQEGCVLDL
                     AVDEGLIQKSGAWYSYGKERMGQGRENAKQFLKEHPEILKSLEQQIKQKYNIITPESE
                     NNTESKEGA"
     gene            complement(7072..7620)
                     /gene="thpR"
                     /locus_tag="PK768_01155"
     CDS             complement(7072..7620)
                     /gene="thpR"
                     /locus_tag="PK768_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HQA59611.1"
                     /translation="MRCFIAIDLDSNVKANIKEFIQQENLNQIFKGIRWVKPDNFHIT
                     LAFLGEITTNQASTVKNILEGIIPKHEPFVIEMSDVGFFPNRRNPRVFWIGIKEQPKL
                     LKLKHDIDKGLTESNINFDKKPFSPHLTLGRIKSSVEIKSETVETLDFKDSFLVKEVS
                     LMKSDLYSNGPIYTELYSFKLG"
     gene            complement(7617..9002)
                     /locus_tag="PK768_01160"
     CDS             complement(7617..9002)
                     /locus_tag="PK768_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQA59612.1"
                     /translation="MSWKRAFVILICAVLAPLVFIHIFDSINVSLQGMELSLGLQLAE
                     KGSTTIHLPPIGVLKAYTHSSPITLKIVLQNVDIDVIKAIIDSIHDKNELISLIKEDA
                     LNAIKILAAKTLLLSISGTFFAAFVLRLKKRDFICCTIISIALVIIILSWTFLSYNVT
                     AFDKPEYFGTLRAAPWLIDIWNKGISQINVLREQLKSMSEGISMVFSRMDSVASTEES
                     MVRVLHVSDIHNNPAAFDFMEQIVQNFNVDFIIDTGDITDYGTYLEDMVIKNLSNLQV
                     KYIFVAGNHDSPNTLEMLEAIDNVIVLDGEMINVKGINVLGFSDARSKTLSIDSSDDM
                     DNLRLKFAIREKLYNLEEKPDILAVHDPQASAALTGFVPIILNGHVHSASLKEENRSL
                     VINAGSSGAAGIRGIQSKADIPYSAVLLYFKSYEALQKPQFFAADIIKISNFKAGFQV
                     ERIFFDQEDEA"
     gene            complement(9003..10244)
                     /locus_tag="PK768_01165"
     CDS             complement(9003..10244)
                     /locus_tag="PK768_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HQA59613.1"
                     /translation="MQAEIIAVGTELLLGQIANTNAQIISNALREIGIDVYFHTCVGD
                     NQKRLMEVLETALKRSDIIVLTGGLGPTKDDLTKETVALYLNLPLIVDDKSLKNIKRF
                     FAERGRPFTENNFKQALIPDGAVAIENKIGTAPGILLNINGKIIILLPGPPQEMELML
                     KDAVIPYLSKTCSSTIFSKVLKFYGIGESALEEKLEDLISQQTNPTIAPLAKAGEVTI
                     RLTAKAENRTAAKNIIKPIEDEIIKRVGDFLYGYDDDSVEEIVAGLLSKFNKTIALAE
                     SCTGGLMAHKLTNIPGISQFFERGIVSYSNRSKEELLNVNPETIRSYGAVSEETAKEM
                     ARGIRISSGTDIGISITGIAGPSGGSIEKPVGLVYIGYSDSNTCYAEKHIFSGRRRDI
                     KERSVNSAFHLLRKMLQRLGD"
     gene            complement(10362..>11331)
                     /locus_tag="PK768_01170"
     CDS             complement(10362..>11331)
                     /locus_tag="PK768_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA59614.1"
                     /translation="IEVIAGIRGRHSSHLEYDQTLNQLLVEMDGISSSDDVNILVIAA
                     TNRPDIIDSALLRPGRFDRIVKVDLPDKEGRLEILKLHTKGKPLADDVDLEQISRETF
                     GFSGAHLENLCNEAAIFAMRENKKQITQKNFLEAIDKVIMGEKLNRKPSNEERRRIAI
                     HESGHALISEHFNPNSVSTITITSRGQALGYIRQIPEDDMYLYTKQYLQSQIAVALAG
                     AVAEEVVLGDISTGASGDFEQATNIAKKMIFSGMSELNIVDKDSLPSEKLHKVVSDIL
                     SEQKLSVQKLIEEKLDILKLVVDYLMENERIDGEKFRELIRTSQVA"
BASE COUNT         3051 a         2398 c         1850 g         4032 t
ORIGIN      
        1 caaagctttg cccgtcagct catctatatc cactattatc ccatttagtt cagctggccc
       61 tttagcaact tcgaatctga caggcaactg attgataaat ttatttatta cgctttcctt
      121 ttttatgcct aataccgaat cgataggtcc agtcatacca acatcggtga tatatgctgt
      181 tccgtttggt agaatttttt catcagctgt ttgcacgtgt gtgtgagtgc ctgcaaccaa
      241 agatacccta ccgtcaaggt accaacccat tgcgattttt tcagatgtgg cttctgcatg
      301 aaaatctaca ataatgattt tagtgatact gctaatttgt tcaataattg catctgcagt
      361 tctaaaggga caatctaaag gtggcataaa ggttctaccg gaaatattta tgatgcctag
      421 tattggacct ttttttattt ccacagtttg atatcctttt ccaggagcaa taggataatt
      481 agcaggcctt atcattcgat tttccgtttc aataaaatta aaaacatctt tattgtccca
      541 tacgtgatta cccatagtca gaaaatcaat accgctaata aagagttcct gagcaatttt
      601 ttctgtaatt ccatttcccc cggcagcatt ttcaccatta gctataacat aatccacctc
      661 atacttatct tttatataca atagtttctc cctaataatt gttctgccgg gtcttcctac
      721 aatatcgcca atcattaata ctctcaacat gttaatatat tcagcccctg tttttatttt
      781 agactttttg ccgtagttga agaaagtgcg caatttgcgc actttacttt gcatattcga
      841 tagctcgggt ttccctgatt acatttactt taatttgacc tggatattcc aattcctctt
      901 ctatcttctt ggaaatgccc ctcgccattt caataacgcc taaatcatct acagcctcag
      961 gctttaccat tatacgaatt tctcgtcctg cttgtatggc ataagacttt tcaacacctg
     1021 caaaggaatt ggcaatttct tcaagttttt caagtctctt aatgtaagcc tctaaggttt
     1081 ctcttctcgc acctggtctt gcagctgata ttgcatctgc tgcttgaact aatatagctt
     1141 ctatggtttg tggttcgaca tcattgtggt gagatgctat ggcatttaca acttcaggag
     1201 attctccgta ttttttagca agttcggctc caactaaagc atgaggccct tcaatgtctt
     1261 gatcgacact ctttcctatg tcatgtaata aacctgctct tttagtgagc ttaatatcta
     1321 cacctaattc cgctgccata gcccctgcca agtgtgcaac ttctatagaa tgatgcaaga
     1381 cattctgacc gtaactggtt ctgaagtata atttgccaag atacttgaca aggtcactgt
     1441 gtaaaccgtg tataccggtg tcaaaggtgg ctttttctcc ttcttctctt attatgttat
     1501 ctacttcttt ctgagctttt tctaccattt cctcaatccg ggcaggatga attcttccgt
     1561 ctaaaatcag tttttctaaa gcaatttttg ctacctgacg cctgatggga tcaaagccgg
     1621 aaagaattac tgcctcagga gtatcgtcga taatcaaatc aatgccggtt aatgtttcta
     1681 aagctcttat gttgcgtcct tctcttccga tgattctacc cttcatttca tcattaggta
     1741 gagtaaccac cgacaccgtc gtttctgcca catggtcggc agcacatttt tgaatggcac
     1801 aggaaataat ttctctcgcc cttttctggg cttcctcttt tgcttgactt tcaatttctt
     1861 taatcatgat agcagcgtcg tgccgtattt cgtcttttac tttgtttaag agaagttctt
     1921 tagcctcgtc ggtagtcaat gctgaaatgc gctcaagttc tgcaatctgc tgcgaataca
     1981 agtcgttaat tttctcatgg agttggttta tttcattttg tttctttgat atggcttctt
     2041 cttttttctc aatagactca agttttctgt caagggcttc ctctttttga attaatcttc
     2101 tttcggttcg ttgcagttca tttcttcttt ctcgctgctc tttttcagct tcatttttta
     2161 gttttaatat ttcttctttt gcttcaatga cagattctct ttttattgat tcggctttgt
     2221 tttcggcatc ttctattatc ttttttgccg attcctctgc cgactgtatt ttagcttcag
     2281 caatatattt gcggattaaa tatccggcag ctaaagctat taatcctact atcaaattcg
     2341 ctaaaatgtt tccactgttg atacttttca cctcctatag gtaaaacaat aaaaaccgag
     2401 gggaactcgg ttttattaat taaatagaga ttactctaat ttaatttaca atcgtgcccc
     2461 cgaacgtcag gctttaagta aagcatagtt tcagtactat tataatttta tactttttta
     2521 acaaccatgt caagaaaaag cggcatgcca cgagaaaaag gaagcgagcc atcgtttgga
     2581 atctagaaga aaacatttgt agctttgttc ctattctgaa ttaagtaccg ttacttcact
     2641 gatgccgttt tttagttcaa ctttatatgc taagtcggat atctcactta aatattgatt
     2701 atgtgttacc ataattatct gtctgtgaaa aacattgctg acctgcttta gaaactgagc
     2761 cacattattt ataaactcat tgctgacatg tttagcaggc tcatctagta ttattggacc
     2821 atctttatac atatcagtgc tttgtataac agcaattctt aaagccagtg aaattacgtc
     2881 gacaattccg ccacctcttg cgtcttgtgg tttcgttcta attttcacat catcatatgt
     2941 cgaaacaaca taaaactctg cactgctttt cccacggaat tcatttaatt caatctcaaa
     3001 ttccaggttt ggtccaaaga caaactggag acaatgggta actagcattt caatttgctg
     3061 tttgatttgt tcccgggcat attcggaagc cctttgaagc aacaatctca cttgttccag
     3121 tatatcaatt gaatttgtca cttcttccag ctcgttttct ttggaagtta aatctttttc
     3181 cagtttctct tttatgcttt ttttcatggc gtgttcaaca attaattgct gcgtatactt
     3241 atcaatatag tctatagatt tatcgaattc tccggtcata tctatccctc tatgttctta
     3301 ggaagtaaat cctttgcctt ctttagtaag tcgttgattt cattgcttat tttttcaatc
     3361 tccttatcca gctcttccgg ttttacattt aatttgttta attcatccag cagagattgt
     3421 cgttgttttt gaagttcttc caggcgcact tcagccttat atctcatggc ttttgcctta
     3481 tcgatttttt gctttaactc gtgaatttct ttctcttggt ccatcatttt cacctcaaaa
     3541 aaatcagttt aaaagatccg atacaatttt ttgcgttgtt tctttatcta ttggcgcaag
     3601 acacgtggga cagatggata atctctcaag taattggctg tattcttttg ccataagggc
     3661 aagatttgac tcaagttttt caagatatgc ctcaccttta ttaatagaag attcaacagt
     3721 taaaattgaa tctttaatat tttgaagtaa tgaaagtttc ttcatcttct gcgacaattc
     3781 agttagatag tgctcggatt tatccatatg gacatattgg gcaatttctt ttgtatggac
     3841 accaagttga taatcaacag atctccattt gtcacggatt gaaatatatc tcttgagagc
     3901 ttctaatagt tttccggctt caagcaataa tatatctgca aagtaagcat tttcggtgtc
     3961 tttaagtact ctttttatgc catttgtatt tctttcatta tctattaaat ttcgtctgat
     4021 attaatgagt ctctcttgct tttgcgctaa ctttaaaata gatgtataat agacttcgat
     4081 agaatctaaa ctttttgttg ctttaacaag ttcctgctca acctttatgg aatctgcagc
     4141 taaagcgagt tttttcatta gttggcataa atagttatat ttactatgca gtgcatcgat
     4201 aattcttata tttttttccg cttcttcaat gaaatctagt tttgacaaaa tacctttatt
     4261 tttttctatt tctatatcgg ttggctccag ctcctgttgt agctgtgtca gtttaaacaa
     4321 gatgtttctc ttttgtttta acttttctaa gataccgctc agttttgaaa tctttttctc
     4381 aagtttatcg acatcgttat aagactgcaa ttcatccctt atattctcta tctcttcttc
     4441 caacagttta tatcgctgct gcttatccac caagtctttt aatgtagtcc gttgagccgc
     4501 atctattatg tgaattccca caagccttcc taaagcttta gccctattgc ttccgctctc
     4561 agatattaaa aaaggtggtt ctagttgttc tgcaagattt actgccgatg tggcatctct
     4621 atctataaaa atttttggta tgccatgagc tttgactatc tctaagggta cggtatttcc
     4681 aaatccctca aaaatatgtt cctggccgtt ttgttctaaa atatatcgat ttcgctggcc
     4741 atctctttct cgaattattt tggagccatc ttgcatctcc agctctacac ggcagtattt
     4801 acaacctgca gttataaaat ctgtccctcg aggttcattg taaagtaccc actttaaagc
     4861 tcgtataata gcactcttcc cctggtctgt ctgtcccaat attaccgtca aaccctcatg
     4921 aaaattgatc tgggtttcct tgtgtgattg aaaattgatt agttttagac tttttatgga
     4981 tcccaaattc agctcacctc aagccaatcc tcttttccca aaagctcctg ggcctggcct
     5041 attctcttta aagcttccat tattacctca tctttaatat tttgctgctt cgaaatttcc
     5101 tcaataattt tattaatatc gaaggtatcg taattgcctg tagaataaac actctgtatg
     5161 aattctgaaa gttttctctc tctaaatgcc atgctctcca ctttagacct atcaagaaca
     5221 tcttcaccgg gaagggcctt gttaagataa atctcttcta ttcttacgtc atcttccaaa
     5281 tccaatagaa taactttcgg catcctcaaa aactcattca aactgttgtt aatcctaacc
     5341 agactccccg gattaatgaa atacttgtta tcaatacatt ttgtagcaaa tcctgcatga
     5401 taatgtccgc ttaaagttat atcagcctcg gtatcgagta tatcttctat cagagtgtag
     5461 gccatgccct caaaaaaagg tttttccagg agcattccat gaactacatg tattgcaaaa
     5521 tcaacatcag ggtcttttct caccacataa cttcttttct catcatctgc atctattccg
     5581 tagaaatagt gctgacctgt cagctgaatc tttttgttat tttctttgaa atacaatttc
     5641 tcaccaggat taattaattt tattaaaccg gcaccgtcta atagacctaa catagtacga
     5701 tttaaagtga gcggattttg accgaagaca tcgtggtttc cagctacagc atatataggc
     5761 aacgaatact tgtctaccaa taatatgaaa tccctaacca gggaaggcgc tatgtcaggc
     5821 ctgtcgaaaa tatccccacc atggagcaat aattgtacat cattgtcttt tgcaatatca
     5881 aaaacctctt ctagtttttg aaataatgtt tctgcgaaat tatcctttct gttttgagga
     5941 ttattgccgc gaatgtgagt atcagtaaaa aaaaggattc gcatatcaag ccccttcttt
     6001 tgattctgta ttgttttcag attcaggggt aataatatta tatttttgtt ttatttgctg
     6061 ctctagactt tttaaaattt ccggatgctc tttcaaaaat tgcttggcat tttctcgacc
     6121 ctgacccatt ctttcttttc cgtaggaata ccaggcacca ctcttttgga taagaccttc
     6181 atcaacggcc agatccagta cacacccttc ctgtgatatt ccttcaccgt agattatatc
     6241 aaattctgcc tgtttaaaag gtggagcgac tttgttttta acaaccttaa ccttagtcct
     6301 atttcccaac atttcatcgc cttgttttaa aatttcgctc ttgcgcacct ctaatcgcac
     6361 cgatgagtaa aactttaaag ctctgcctcc aggggttact tcaggatttc cgaacataat
     6421 acctactttt tctctcagtt gatttataaa aattgccacc gtctttgact tgttgatagc
     6481 ccctgccagc ttccttagcg cttgagacat aagtctggcc tgcaagccga catgggcgtc
     6541 tcccatttca ccatctattt cagcttttgg aacaagtgct gctactgagt caattacaac
     6601 tacatctata gcaccacttc gaacaagggc ttctgctatt tccaaagctt gttcacctgt
     6661 atcaggctga gaaatcagca aattgtcagt gtccacacct aaattttttg catagtttgg
     6721 gtctagggca tgctccgcat caataaatgc agcggtaccc cccatttttt gtgcttcagc
     6781 gactatatgt aaagcaacgg tagtttttcc tgaagactcc gggccatata tttctattat
     6841 cctaccccta ggaacaccgc ctatgcctaa tgctaaatcc agtggcaatg cgccagttgg
     6901 aatagcttca atattaaatt ttgatgcagc atcgcccaat ctcattattg aacccttgcc
     6961 gaactgcctt tcaatttgag caatagcggt ttctagggct ctatcttttt ctgccatatt
     7021 taaccctcca aactttttta ttgtgcatat ttattcaaca taagtaaaaa tttagccaag
     7081 tttgaaactg taaagctcgg tataaatcgg gccgtttgaa tacaaatcac ttttcattaa
     7141 tgatacctct tttaccaaaa aggaatcctt aaaatctaaa gtctctacag tttcggattt
     7201 aatctctacg gaagatttaa ttctgcccag tgtcagatga ggtgaaaaag gctttttatc
     7261 aaagtttata ttactctctg taaggccttt gtctatatca tgttttaatt ttaggagttt
     7321 gggctgttct tttataccaa tccaaaacac ccttggattt cttctattcg ggaagaatcc
     7381 aacatctgac atttcaatga caaatggttc gtgtttcggg ataatacctt ctaagatatt
     7441 cttaacggtt gatgcctgat tagttgtgat ttcacctaag aaagcaaggg tgatatgaaa
     7501 gttatcaggc tttacccacc ttatgccttt gaaaatttga tttaaattct cctgctgtat
     7561 aaattcttta atattagcct taacgtttga atccaaatct atggctatga agcatctcat
     7621 gcctcgtcct cctggtcaaa gaaaattctt tcaacttgga aacccgcttt aaaattagaa
     7681 attttaatta tgtctgcagc aaaaaattgc ggtttttgca gcgcttcata agacttaaaa
     7741 tataataaga ccgccgagta aggtatatca gctttcgatt gaattccgcg tatacctgca
     7801 gctccgctac ttcctgcatt tattactagg gatctatttt cttcttttaa agaggcacta
     7861 tggacatgac catttaaaat aattggaaca aatcctgtta aagctgctga agcttgaggg
     7921 tcgtggactg ctagaatatc cggcttttct tctagattat acagtttttc tcgaatggca
     7981 aattttagtc ttaagttgtc catgtcgtcg gaagaatcaa tgcttagcgt ctttgagcgc
     8041 gcatcagaaa aacctaaaac gtttatgcct tttacattaa tcatttcgcc atctaagacg
     8101 atcacattat cgattgcttc tagcatttct agagtattgg gagaatcatg atttccggct
     8161 acaaaaatat acttgacctg caaatttgac aaattcttta taaccatgtc ttctaaatag
     8221 gtgccgtaat cggtgatatc tccggtatca atgataaaat cgacattaaa attctgaact
     8281 atctgctcca taaaatcaaa agcagcagga ttgttatgaa tatcgctgac atgcaagacc
     8341 cttaccattg actcttcggt ggaagcaacg ctatccattc gagaaaagac cattgaaatg
     8401 ccttcagaca tactcttcaa ctgttcccgc aatacattaa tctgtgatat gcctttattc
     8461 cagatgtcga taagccaggg agcagctctc aatgtcccaa aatactcggg tttgtcaaaa
     8521 gccgttacat tgtacgataa aaaagtccaa gacaatatta taataactag tgctatactg
     8581 attatggtgc agcaaataaa atctcttttt tttaatctta atacaaatgc agcaaaaaat
     8641 gttccggaga tgcttagaag taaggttttt gcagcaagta tcttaatggc atttagtgca
     8701 tcttctttta ttaatgaaat caactcattt ttatcatgta tgctgtcaat gatggctttt
     8761 ataacatcta tatctacgtt ttgtaaaaca atttttaagg taatgggaga actgtgagta
     8821 taagctttga gaacacctat aggcggtaaa tgtatggtag ttgagccttt ttctgccaat
     8881 tgcaaaccca aagaaagctc catcccctgc agacttacat ttatactgtc aaaaatgtgg
     8941 ataaaaacta agggggctaa aacagcacag attaaaataa caaaggctct tttccaactc
     9001 atttaatcac ctaagcgttg caacattttc ctaagcagat gaaaagcgct gtttactgag
     9061 cgttctttta tgtcccttct tctaccgcta aaaatatgtt tttcagcata gcatgtattg
     9121 gaatccgaat atcctatata taccaggcct acaggtttct cgatgcttcc accggagggg
     9181 cctgctatac cggtaattga aatcccgata tcagtgcctg atgaaattct gatacccctg
     9241 gccatttctt tggctgtttc ctcacttacg gcaccgtatg accttatagt ttcgggattt
     9301 acatttaaca attcctcttt tgagcgatta ctgtaagaaa ctatacctct ttcgaaaaac
     9361 tgcgatatcc cgggaatatt tgtgagtttg tgtgccatta aacctcctgt acaggactct
     9421 gctaaagcta ttgttttatt gaatttagat aataatcctg ctactatctc ctctacagaa
     9481 tcgtcatcat atccgtataa aaagtcgccc actcttttaa ttatctcatc ctcaatgggt
     9541 tttattatgt ttttagcagc agtgcgattt tcggctttgg cagtaagtcg aatcgtcact
     9601 tcccctgctt ttgccaatgg tgcgattgtt ggatttgtct gctgagatat caaatcttcg
     9661 agcttctctt ccaatgccga ttcaccgatt ccgtaaaact ttaagacttt agaaaatata
     9721 gtgctactac acgtcttaga aagatagggt atgaccgcat ctttgagcat taattccatc
     9781 tcttggggag gacccggaag caagataatt atctttccat ttatatttag tagtattccg
     9841 ggggcggttc ctattttgtt ctcaatggct acagccccgt ctggtatcaa agcttgcttg
     9901 aaattgtttt cggtaaaagg ccttcccctt tcggcaaaaa atctcttgat gttcttcagt
     9961 gatttatcat caacaattaa cggaaggttt aagtaaagag ccacggtttc ctttgttaga
    10021 tcatccttgg ttggccccaa accacccgtt aaaacaatga tatcggaacg ctttaatgct
    10081 gtttccaata cctccattaa ccttttttga ttatctccca cacaagtatg aaaatagacg
    10141 tcaattccaa tttcccgtaa agcattggat atgatttgag catttgtatt tgctatctgt
    10201 cctaatagca gttccgtacc tacggcaatt atttcagctt gcaagtcttg tcaccttcaa
    10261 acaaatatga tgaataaata atgctaaatt aattattcgt tttttttaag gaatatcctt
    10321 tttttattca aacaaaagtt tgactaaaaa agacacttaa tctacgcaac ttgggacgtt
    10381 cgtattaact ccctgaattt ttcaccatct attctttcat tttccatcag ataatcaact
    10441 accaatttca agatgtctag tttttcctct atcagctttt gtactgataa tttttgttct
    10501 gacaatatgt ctgaaacaac tttgtgtagt ttttctgatg gcaatgaatc tttgtcaact
    10561 atatttaact cagacatccc agaaaatatc atcttttttg ctatatttgt ggcttgttcg
    10621 aaatcaccgg atgcacctgt gctgatatca ccgagcacca cttcctctgc tacggctccc
    10681 gctaaagcca cagctatttg tgattgcaga tattgctttg tgtataaata catgtcatct
    10741 tccgggattt gcctgatata tccgagagcc tggcctctcg aagttatcgt tattgtggaa
    10801 actgaatttg gattaaagtg ttcgctgatt aaagcatggc cgctttcatg aattgcaatg
    10861 cgcctacgct cttcattgct aggttttctg ttgagttttt cacccatgat aactttatca
    10921 atagcctcta agaaattttt ctgtgtaatt tgttttttat tctctctcat ggcaaaaatg
    10981 gcagcttcgt tgcagagatt ttccaagtgg gctcctgaaa aaccaaaagt ctctctagat
    11041 atctgttcaa ggtccacatc atcggcaagg ggtttgccct tggtgtgcaa tttaagtatc
    11101 tctagtcttc cttctttatc cggaaggtct acttttacaa ttctatcaaa tctgcccggt
    11161 ctaagtaggg ccgagtctat gatatcgggt ctgtttgtag cagcaatgac gagtatattg
    11221 acatcatcag atgaggaaat accgtccatt tctactaata attgatttaa ggtttggtca
    11281 tattctaaat ggctgctatg cctgccgcga attccggcaa taacttctat c
//
LOCUS       DAONUO010000016        11182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4491_length_11182_cov_4.002337, whole genome shotgun sequence.
ACCESSION   DAONUO010000016 DAONUO010000000
VERSION     DAONUO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4491_length_11182_cov_4.002337"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK768_01175"
     CDS             complement(<1..207)
                     /locus_tag="PK768_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59615.1"
                     /translation="MNSLYLKVLKVDSSEAVINKFYDTLIDTNTRKNRHYVPLSFYVM
                     NLFLKNGFVLKEDIIKVQHNCKSTP"
     gene            complement(223..1182)
                     /locus_tag="PK768_01180"
     CDS             complement(223..1182)
                     /locus_tag="PK768_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HQA59616.1"
                     /translation="MQKSKLQSLEKGKTHKVEAKPFVKWAGGKTQLLDELASRLPFII
                     KESKSIERYVEPFVGGGAFFFYLKGNYEVKEAFLYDINVELTVGYKVIQKDVYDLIYQ
                     LEKIEKEYLKLPEEERKKYYYRTRDEYNRQQETFDYENYNGDWIKRAAYLIFLNKTCF
                     NGLFRQNKKGKFNVPFGRYKKPTICDRENLLNVHVALNNTHIFAADFEESKKFITENT
                     LVYFDPPYRPLNETSNFTSYDKDGFSDEDQIRLSEFYREMDKRGAFLLLSNSDPKNVD
                     INDDFFDSLYAGFIIERVNAKRFINCDGSKRGDIKELLIRNYE"
     gene            complement(1389..6266)
                     /locus_tag="PK768_01185"
     CDS             complement(1389..6266)
                     /locus_tag="PK768_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQA59617.1"
                     /translation="MPLNPITATKEIEKRYSQYLSTTFYINDAELQNKFLHELSQKNR
                     FIKGPILEATPPFKKGKNIEDLIIEGVLCEEFKKLNSPDLPIQRGLYLHQEKAVRKII
                     TEKRNIIAATGTGSGKTEIFMIPILNYLFTQKEKGKLTHGVRALLLYPMNALANDQLK
                     RMRNLLHNYPDITFGSYTGETEERERDALEKYRKMFNGDEPLKNELLSREKMKKQPPH
                     ILITNYAMLEYLLLRPDDNVFFDGPYAGNWKFIVLDEVHTYTGAKGIEMSMLLRRLKE
                     RVLTDNSPKLQFIGTSATIGRGKKDFQKVAEFGEKLFGEEVSWEDFNERKQDIIYGER
                     LPVEISKESWGKPDAVLYERLCSIIERSDRVDIVNSLLETARDHRVPLQVINMARENN
                     PKGGYHGVLYEILKGDSRIIELQQHLEKGPCYLSDVSKVIFPSEKAPRDKLVYLIQLA
                     NAAFPEDGYRPLIPARYHLFVRAIEGAYLILVPEERIFLEKREHVNINNHQYPAFEIA
                     SCRNCGALYLVGKTIDGEENRKVLKQHGKLHIEEDVSLEYYLLLRDNQEYEYINEDEL
                     ALGYRFDEYKEMYKLCGKCGAIDKDNLVGKMCGCGEENYIKLLKVDSKEGLVHKCPAC
                     GSVQPQGSIVWRFLFGVDAVTSVLATALYQQGEDTGTSLSEEQIHQEEDEWGTLSTDN
                     SFVGESKDQKVKGGQLLVFSDSRQDAAFFATYLSNSYSQILRRRMIIKTLEKNKDKAI
                     SNRWRLVDLVNHLKKYLEETELFPNKSPQQLEDEAWKWVLYEFFAFDRRTGLEGLGLL
                     GFSLLKPKVNFRNPFMAEPWNLTFEESWMLFQVLLDSFRKNAAIVFPPNISPQDEFFA
                     PRNREYYFKQKSNTSGIFGWCPSKGINSRLDFLMRLAKNGLNLNISKKECAQLLEEIW
                     SKGLRLDNPKSIFYSYFSSVHNSSDGTLYRVKTDYWELRPGIIDKSITWYFCPVCHRV
                     TLYNIKGVCPTFKCPGKLEKCDPDTLFKNNHYRNLYMSVLPMWMNSREHTAQLTTEAA
                     AKIQQQFNEGRVNVLSCSTTFELGVDVGELETVFLRNVPPSPANYIQRAGRAGRRKNS
                     SAFILTFAQRKSHDLTYYNNPKRLILGEIMPPYFELKNEKIISRHVFSIAFSKFWREH
                     ADFFGSVENFFYNYKDGVELFKNFLIRRPMDLLASIKTVVPKYMHKQLEIDEWGWVNK
                     LIDDEKGLLSWAKTELISDVKQLESMQNYYVKKKKYRKADLMNKTINTLKRRSLINYL
                     AQKNILPKYGFPVDVVDLQIYHHGDEAKQLDLNRDMQLALSEYAPGSQVIAAGLLWTS
                     RYLKKLSDRELVKRIYAICDHCGNYESTLYDDKQQIGSCSACGNRIGRNRGVFVIPEF
                     GFISENPKAPTMEKPRKTYTTRKYYAEGGTEDTRTDVDFNGIHAELISMANGRLAIIN
                     HAGYKGFLVCEKCGYAVVNDGIKDFTHNTAYGEKCNGSWQRVALGFEFHTDILQIRFK
                     NYNRHEDGFWLSVLYGILEGVSYSMEIERTDIDGCLYAYSGDPTSPALVLFDSVPGGA
                     GHVKRIIQENNFEEVLKATLKIVSECTCGGEEGGTSCYGCLRNYSNQFCHDVLDRNKV
                     KEFMEEMLGKKYIRD"
     gene            complement(6288..10250)
                     /locus_tag="PK768_01190"
     CDS             complement(6288..10250)
                     /locus_tag="PK768_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59618.1"
                     /translation="MKNLREWEKHLASLVKNVELLGDIELTLEEFKELTQELKEFTKE
                     YSYISLKNYAPLSLAVFLVRIGIEEYSEGNYWSAVQQTLGLPIDDPRWQRTFGDSFLN
                     TLDEFAFPKFDKAGLKYVTPILAHGRVPNNYLENYFDVIFGFFTEKLKDDLSKDYIRN
                     TVELWREDHSGRIGLQTKLDKEKAELKKVNDELNKHSNIIEYLPLYEKAKEISNRIIE
                     ADELAYLLALPEDFCKNAEIELEKLNQELHGKQNLLKAIVDFKKERSEKFARLTAKQH
                     VLDEIIDSIEMFTEKFEKPISENIGREILALPLDELEAAYQNYTSSSQRNIFYNIKIF
                     FLEIIQRLFGKKKQEEKYAAEIKELVADIPFKESILLNIDEVYRITKDLRTLFTEYFT
                     YKREIESLEIALEQIKSGFLETAAGKVPSIILDDSDEITVQLEKDIKRLHNKIIDLEK
                     EINDYKTKVVALGKGNFEKGKEILAEQRKYRKDYLEISEQIGTDRSFEIFERNTDITT
                     LKTHIEELKKKRNALIKLAEVTEKKLQSYPEPPLYNLNESAKKFIYFGEDISIDFIYE
                     TANMLKSFLYGEDLTQVNLPVRIQESAKEWIMKHGEELEKIKHIESTEPEHLFRTPSI
                     YYDQEIREIRVNLPEQRFSKALVSEDRVSIKIFKDDYRALIDGVTVRLYSMDTFFLTE
                     EKNIILTEPAKTLTIELRSGEKIIQTWSIPLMFDDNPFIIFDQKGKKIDTDKVFANER
                     AILILDNKFKIGPSEIIEGEENLYDGWFDYKSCYLNLTDASQIVLLKDDDVYTLFRKP
                     DRLKPTLIGQNMLRNITIKDSPVYLDSPPEIIFSEAAMQLRLWQLILKVDEIEKYYLL
                     DDLTGLKEVKDDIIRLPLSKLTNNKFGTYQLFLEKRGVGGYKCEFEFSVLPNTSFEFD
                     KEFYEPNYSNKRCSLQIKSSYSILFAPLSSSYIVEAKTNKECIVKLTPDRDTIAGDLS
                     YQIRGSNLTFPIRISIPAIGYMYDDSESWNYEIQELWHEDIENLRVFIPIDNISKVVL
                     YINDREQESIASIANKIATFDLAIFKDTFRENKKSVQELFIEFPERRDLTAILLNRIR
                     TRWEVSDVSIKQDVVEKKRILIISWEEKGKAANRAIVFWEISSGAKLFEFKLEDDTHE
                     FVIEKPLDDFPDGAYRIQFLEIDPWETAVSIMPESDEINTSDVLIGEITVPIDKIIQH
                     GIRVAFFSDDQNGNLYTVYPANAPLINDIKQIKGEDIEAEYQGMFYQVNEDGELLPMP
                     TNPVRFMINEDMSLAYLIDRDRDGPMYCPKCREIFWEIREKHKRHHIEPRKIFFYLNR
                     "
     gene            10765..>11182
                     /locus_tag="PK768_01195"
     CDS             10765..>11182
                     /locus_tag="PK768_01195"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQA59619.1"
                     /translation="MSGELITIDLEKLKGYVTSKLAKFPEIQGVYLFGSSLDKMRPDS
                     DIDLGIIFDEKLSEDIDLLIEKVYYALGRFDGHSFDIVSVRDVNAILSFRIFHQGMPL
                     YIADNDKVTDLMERVSRIYSDIYPRYRRALEIISGLR"
BASE COUNT         3080 a         2244 c         1861 g         3997 t
ORIGIN      
        1 tggtgtagat ttgcagttgt gttgaacttt tatgatatcc tcttttaaga caaatccgtt
       61 tttcaggaaa agattcatta cataaaaaga tagaggcacg tagtggcggt tttttcttgt
      121 gtttgtatct atcaatgtgt cataaaactt gtttatcaca gcttcagaac tgtcaacttt
      181 tagtaccttt aagtataatg agttcatgaa aagaccccca tactattcat agttccttat
      241 aagtaattct tttatgtccc cccgctttga accatcacaa ttgataaatc ttttggcatt
      301 tactcgctct attataaaac cggcataaag gctgtcaaaa aaatcgtcat ttatgtctac
      361 atttttaggg tcagaattgc ttaatagcaa aaaagctccc cttttatcca tttctctata
      421 aaattcagat aaccttattt gatcttcatc tgaaaacccg tctttatcat agcttgtaaa
      481 gttagaagtt tcatttagtg gcctgtatgg agggtcaaag tatactaatg tattttctgt
      541 aataaatttt ttactttcct caaaatcagc agcaaatata tgagtattat taagggccac
      601 gtgaacattg agcaggtttt ctctatcgca tatggtgggt tttttgtacc tgccaaaggg
      661 tacattgaat ttcccctttt tgttttgccg gaataacccg ttaaaacagg ttttgtttaa
      721 aaatattaaa taggctgccc tttttatcca gtcgccgtta tagttttcgt aatcaaaagt
      781 ttcttgctgc cggttatact cgtctctagt tctgtaataa tactttttcc tctcttcttc
      841 aggcaatttc agatattcct tctctatttt ttcaagctgg taaatcaaat cataaacatc
      901 cttttgaatg accttatatc ctacagttaa ttccacattt atatcatata aaaacgcttc
      961 ttttacctcg tagttgcctt ttaaataaaa gaaaaaggca cctcctccta caaaaggttc
     1021 tacgtatctt tctatagact tcgactcttt tattatgaat ggcaacctac tggccagttc
     1081 atccaaaagc tgggtttttc cacctgccca ttttacaaac ggtttggctt cgactttatg
     1141 ggtcttgcct ttttctaatg attgtaattt gcttttttgc attttctatc acgtcctatg
     1201 tttgtatata tctttcagtc attatatcat atatttggta tagatatttt ttcgtcaaat
     1261 aattacaaat tccttctatt cgggtatagt tacgaaagaa atgagataga acaaaaacac
     1321 cgctataaag cggtaaaatt atatttctag agtctatttt tataatgaat tctgctagac
     1381 ctacgtatct aatctctaat gtatttttta ccgagcatct cttccataaa ttctttaact
     1441 ttatttctat ccagcacatc gtggcagaat tggttggaat agttcctcaa acaaccgtag
     1501 caacttgttc ctccttcttc gccgccacaa gtacattctg agactatctt tagagtcgct
     1561 tttaaaactt cttcaaaatt gttttcttgt attatgcgtt ttacgtggcc tgcgccgcca
     1621 ggaacactat caaataatac aagggcaggg ctcgtggggt ctcctgaata ggcataaagg
     1681 cagccatcga tatctgttct ttctatttcc atggaataac tgacaccttc taaaatgccg
     1741 tacaaaacag aaagccaaaa gccatcttcg tgcctgttgt agtttttaaa acgtatttgc
     1801 aaaatatcag tatggaattc aaagccgagg gcaacccttt gccaggaccc attgcatttt
     1861 tccccgtatg cagtattgtg ggtaaaatct ttgatgccat catttacaac agcatatccg
     1921 catttttcgc atacgagaaa acctttgtat ccagcatgat tgattatggc caatcttccg
     1981 tttgccatgg atattagttc tgcgtgaatt ccattaaaat ctacatccgt tctggtatcc
     2041 tcggtgcctc cttcggcata atattttctg gttgtataag tctttctggg tttttccatg
     2101 gttggagctt tggggttttc tgaaataaaa ccgaactcgg gaatgacaaa gacacctcta
     2161 tttcgaccta ttctgttgcc gcaggcagag cagctgccta tttgctgctt atcgtcatat
     2221 agggtgcttt catagttccc acaatgatca cagatggcat agatcctttt aaccagttcc
     2281 ctatcagata atttctttaa ataacgactg gtccacagaa ggccagctgc tataacttga
     2341 cttcctgggg catattcgga caatgctagc tgcatgtcgc ggtttaaatc tagctgtttc
     2401 gcctcatcac catggtgata tatttgcaga tccactacat caacaggaaa gccgtatttt
     2461 ggaagtatgt ttttttgagc taaatagttt atcaagctcc ttcttttaag ggtgtttata
     2521 gttttattca ttaggtctgc tttacgatac tttttcttct tcacataata gttttgcatt
     2581 gattccaatt gctttacatc actgatgagc tcggtcttcg cccaactgag taggcctttt
     2641 tcatcatcaa taagtttatt tacccaaccc cattcatcaa tctctagttg cttgtgcata
     2701 tatttgggca ccacggtttt aatcgatgca agtaaatcca ttggacgcct tatcaaaaag
     2761 tttttgaaaa gctctacccc atctttgtaa ttgtaaaaga agttttcaac actgccgaag
     2821 aaatcagcat gttccctcca aaatttggaa aatgctattg aaaaaacatg tcttgagatg
     2881 attttttcat tttttaactc aaaataaggt ggcatgattt cgcctaatat gagtcttttt
     2941 gggttgttat aatatgttaa gtcatgggat tttcgctggg caaaggttag tatgaaggct
     3001 gatgaatttt tccttcttcc agctcttcct gccctttgaa tgtaattggc cggtgatgga
     3061 ggaacattcc tcaaaaagac agtttccagt tctcctacat ctactccaag ctcaaaggta
     3121 gtagaacagc taagcacatt tacacggcct tcattaaact gctgctggat tttcgctgcg
     3181 gcttcagttg tcagctgggc agtgtgctcc ctgctattca tccacatggg caatacactc
     3241 atgtaaagat ttctatagtg attatttttg aacaatgtgt ctggatcgca tttttccagt
     3301 ttgccggggc atttgaaggt ggggcaaaca ccctttatat tgtacaaagt cactctgtga
     3361 cacacgggac agaagtacca agttatgctc ttgtcaataa ttcctggcct gagctcccaa
     3421 taatcggttt ttactctata caaagtccca tctgacgaat tatgcactga agaaaaatag
     3481 gaataaaaaa tactcttggg gttgtcaagc cgtagtccct ttgaccaaat ttcctctaat
     3541 aattgagcac actctttttt tgatatattt aggtttaaac cgttttttgc aaggcgcatt
     3601 aaaaaatcca accgtgaatt tatgcctttt gaaggacacc agccaaaaat tccacttgta
     3661 ttgctttttt gtttaaagta gtattcgcgg tttctaggtg cgaagaattc gtcctgagga
     3721 gatatattcg gcggaaacac aatagcagca tttttcctga aactgtcaag taatacttga
     3781 aataacatcc aactttcttc aaaagtaaga ttccaaggct cagccataaa aggatttcta
     3841 aagtttactt tgggttttaa tagtgaaaag cccaacaggc ctaagccttc caagccagtt
     3901 cgcctatcga aagcaaagaa ttcgtataac acccatttcc aagcttcatc ttctagttgc
     3961 tgagggcttt tatttggaaa cagctcagtc tcctctaaat atttttttaa atggttaacc
     4021 aagtcaacta gacgccatct gttggatatt gccttgtctt tgttcttttc taaagtcttt
     4081 attatcatcc tacggcgcag tatttgtgaa taagaattgc tcaaatatgt agcgaaaaag
     4141 gctgcatcct gccggctgtc agagaagact aaaagttgtc ctccttttac cttttggtct
     4201 ttcgattctc ctacgaaaga attatccgtt gataaggttc cccactcatc ctcttcttga
     4261 tgtatttgct cttcagataa gcttgttcct gtgtcttcac cctgttgata cagcgcagtt
     4321 gcgagaacac ttgtgactgc atcgacacca aacaaaaatc tccaaacgat ggaaccctgt
     4381 ggctgcacgc ttccacaagc gggacattta tgaactaaac cttctttaga atcaacttta
     4441 agcagtttaa tgtaattctc ttccccacat ccacacattt tgccaacgag gttatcttta
     4501 tcaatagcac cgcatttacc gcaaagctta tacatttctt tgtattcatc aaagcggtat
     4561 cctaatgcca attcgtcctc attgatgtat tcatactctt gattgtccct tagtagcaaa
     4621 taatattcca aacttacatc ttcttcaata tgcaacttgc cgtgttgttt aagcaccttt
     4681 ctgttttctt ctccgtcaat agtcttcccc acaaggtata atgctccaca attccggcag
     4741 ctggcaattt caaaggcagg atattgatgg ttatttatat taacatgctc tctcttctct
     4801 aaaaaaattc tttcttcagg aactaatatc aaatatgcac cttctattgc tctgacaaat
     4861 aggtggtatc tggcaggtat aaggggtcta tatccatctt caggaaaagc tgcattggcc
     4921 agctgaatca aatatactag tttatctcta ggggcctttt ccgatggaaa gattaccttt
     4981 gatacatcac taaggtaaca tggccctttt tccagatgct gttgcaactc gatgattcta
     5041 ctatcaccct tgagaatttc gtaaagcacc ccgtgataac cgcccttagg attgttttct
     5101 ctggccatat ttataacttg taaaggcact cggtggtctc tggcagtttc cagcaaactg
     5161 tttactatat caaccctgtc agatctttca ataatgctgc acagcctttc ataaagaact
     5221 gcatctggtt taccccagct ctctttagat atttcaacag gcaatctctc gccgtaaata
     5281 atatcctgct ttctttcatt aaaatcttcc caagaaactt cttcgccaaa aagtttttcc
     5341 ccaaactcag ctactttttg aaagtccttt ttccccctac caatggtggc gctagtacca
     5401 atgaactgaa gtttcggaga gttatcagtt aatactcttt cttttaatct ccttagcagc
     5461 attgacatct cgataccttt ggctccggtg tatgtatgaa cctcatccag tacgatgaac
     5521 ttccaatttc cagcatatgg cccgtcaaag aaaacattgt catcaggacg tagcaagagg
     5581 tattccaaca tggcatagtt ggttataagt atatggggtg gctgtttttt cattttttct
     5641 cgggacagca gctcattttt caacggttca tccccattaa acatcttcct gtatttttct
     5701 aaagcatctc tttctctttc ttccgtctct ccggtataac ttccaaaagt tatatcaggg
     5761 taattgtgca agagattcct catccttttt aattggtcat tggctaatgc gttcatggga
     5821 tataggagta atgccctaac tccgtgagtg agctttccct tttctttttg agtaaaaagg
     5881 taatttaata tgggaatcat aaatatctct gttttaccac tgccagtccc tgtagctgct
     5941 atgatgtttc gtttttccgt tataattttt cgaactgctt tttcttgatg gagataaagc
     6001 cccctttgga tgggcaaatc aggagagttt agtttcttaa attcttcaca aaggacacct
     6061 tcgataatta aatcttcgat gtttttacct tttttaaaag gtggtgtagc ctctaaaata
     6121 ggtcctttga taaaacggtt tttttgggac agttcatgca aaaatttatt ttgaagctca
     6181 gcatcattaa tataaaaggt tgtagataag tactgtgagt acctcttttc aatttctttg
     6241 gtggcagtaa tagggtttaa aggcatatac aagaccccct ttttccctta tcgattaaga
     6301 taaaagaata tctttctcgg ctctatgtgg tgtcttttat gtttttctct tatctcccaa
     6361 aaaatttctc tgcattttgg gcaatacatt gggccatctc tatcacggtc aattaggtag
     6421 gccagggaca tgtcttcgtt tatcataaat cttactggat ttgtaggcat cggcaataat
     6481 tcaccatctt cattcacctg ataaaacatt ccctgatact ctgcttctat gtcctctcct
     6541 tttatttgct taatgtcatt tattaaaggt gcatttgccg gatatacagt ataaagattt
     6601 ccgttctggt catcactgaa aaatgctact cgtattccat gttgaatgat cttatcaata
     6661 ggtacggtaa tctccccaat tagcacatcg gaagtgttta tttcatcact ttctggcata
     6721 attgaaacgg cagtctccca agggtctatt tcaagaaatt gtatcctgta agctccatca
     6781 ggaaaatcgt cgagcggctt ttcgataaca aattcatgag tatcgtcttc taatttaaat
     6841 tcaaacagct tagcccctga tgaaatttcc caaaaaacaa tagccctgtt tgcagctttg
     6901 cctttttctt cccatgaaat tattaatatt cgttttttct caactacatc ttgtttaata
     6961 ctgacatcac taacttccca tctggtcctg attctattta gcaatatagc tgttaaatct
     7021 cttctttcag gaaattcaat gaacagttcc tgaacagact ttttgttttc tctaaatgta
     7081 tctttaaata tggctaagtc aaaagtggca attttgttgg ctatgctagc gatactttct
     7141 tgctcacgat catttatgta taataccact ttgctgatgt tatcaatagg tatgaaaact
     7201 cttaaattct caatatcctc atgccataat tcctgtattt cgtaattcca gctttcgcta
     7261 tcatcataca tatatccaat ggcgggaatc gatattctta ttggaaatgt caggttactt
     7321 cctctaattt gatagctaag atcgccggca attgtatcac ggtctggtgt aagttttaca
     7381 atgcattctt tattagtttt ggcctccacg atatagctgg aggacagcgg tgcaaaaaga
     7441 atgctgtagg aagatttgat ttgtaagctg catcgtttat tggaataatt cggttcgtaa
     7501 aattctttat cgaattcaaa agaagtattg ggcaatactg agaactcaaa ttcacactta
     7561 taacccccga cacccctctt ttctaaaaac aattgatagg taccgaattt attattagtc
     7621 aatttagaaa ggggtaatct gattatgtcg tcttttacct cttttaaacc tgttaaatcg
     7681 tctaataaat aatacttctc tatttcatcc actttcagaa ttaactgcca caaacgcaat
     7741 tgcattgctg cttctgagaa aataatttca gggggtgagt caaggtacac cggagaatcc
     7801 tttattgtaa tattgcgcag catgttttgg ccgattaaag ttggctttaa cctgtcaggt
     7861 tttctaaaga gcgtatatac atcatcatct ttaagtaaaa cgatttggct agcgtcagtt
     7921 agatttagat aacagctttt ataatcaaac catccatcat acagattttc ttcaccttcg
     7981 attatttctg aaggtccgat tttgaatttg ttatctagaa ttaatattgc tcgctcattg
     8041 gcgaaaactt tgtctgtatc tattttcttg cctttttggt cgaatataat gaatggatta
     8101 tcatcgaaca tcagaggtat tgaccaagtt tggataatct tctcaccgct tctcagttca
     8161 atagttaaag tctttgctgg ttcggtcaga ataatatttt tctcttcagt taaaaagaat
     8221 gtgtccatag aatacagtct tacggtcaca ccatctatca atgcccgata atcatcttta
     8281 aaaattttaa tagaaactct atcctctgat actagagcct tcgaaaaacg ttgttccgga
     8341 aggtttactc tgatttctct tatctcttgg tcatagtaaa tgctaggagt tctaaacaaa
     8401 tgttctggct ctgtgctttc aatatgttta atcttttcaa gttcctctcc atgtttcatg
     8461 atccattctt ttgcagattc ttgtattcga actggcagat ttacctgtgt taaatcttcc
     8521 ccgtataaaa agcttttcaa catattggcc gtctcataaa taaaatctat agatatatct
     8581 tcgccgaagt agatgaattt ttttgccgat tcgttcaggt tataaagggg gggttccggg
     8641 tagctttgaa gctttttttc ggtaacctcc gcaagtttga ttaatgcatt tctcttcttt
     8701 ttaagttctt caatatgggt tttcagtgtg gttatatccg tgttcctctc aaatatttca
     8761 aaagacctat cagtgccaat ttgctcgctg atttctaaat agtcttttcg atattttcgt
     8821 tgttcagcaa ggatttcttt gcctttttcg aaattacctt tccccaatgc gacaactttt
     8881 gtcttatagt catttatttc cttttccaaa tctatgattt tgttatgtag tctttttatg
     8941 tctttttcaa gctgcacagt aatttcatca ctatcgtcaa gtatgattga tggaactttt
     9001 cctgctgctg tttccaaaaa gccacttttt atttgttcta atgcaatctc caatgattct
     9061 atctctctct tatatgtaaa atactcggta aataatgtcc ttaaatcttt ggttatcctg
     9121 tagacttcat cgatatttag caatatactt tctttaaagg ggatatctgc gaccaactct
     9181 tttatctctg ctgcatattt ttcttcttgc ttcttttttc caaaaagtcg ttgtataatt
     9241 tcaaggaaaa atatcttaat attgtagaag atgtttcttt gggatgaaga agtgtagttt
     9301 tgataagctg cctctaactc atctaatggc agtgctaata tttctcttcc gatattttcg
     9361 gaaattggct tttcaaattt ttcagtgaac atttcgatac tatcgataat ttcatccaaa
     9421 acatgttgtt ttgcggttaa acgcgcgaat ttctcagagc gttctttttt aaaatctaca
     9481 atagctttta gtaagttctg ttttccatgt agttcctgat ttagcttttc taattctatc
     9541 tctgcatttt tgcaaaagtc ttccggtaat gcgagtaagt aagctagttc atcggcctca
     9601 attatcctat tggatatttc ttttgctttt tcatataaag gcaagtattc tataatattt
     9661 gagtgtttat taagctcgtc atttactttc tttagttcag ctttttcttt atccagtttt
     9721 gtttgtaaac ctattcttcc gctatgatct tccctccaca attctacggt gtttctaata
     9781 tagtctttag agagatcatc ctttagtttt tcagtgaaaa agccaaagat aacatcaaaa
     9841 taattttcta gatagttgtt tggaacccga ccatgggcaa gtataggtgt gacgtatttt
     9901 aaacctgctt tatcaaattt gggaaaggca aattcatcaa gagtgtttaa gaatgaatct
     9961 ccaaatgtcc tctgccacct tggatcatcg attggaaggc ctaatgtctg ctggactgcc
    10021 gaccaataat tgccctcact gtattcttct ataccgattc tcactaaaaa aaccgccagt
    10081 gataacggtg cataattttt taaagaaata tagctgtatt ctttggtaaa ttcctttaat
    10141 tcttgagtga gttctttaaa ctcttctagt gtaagctcta tatctcctaa taactccaca
    10201 tttttaacca atgatgcaag atgtttttcc cactctctga ggtttttcat tggcaagcca
    10261 ccccttacat acttgactct gcatccttaa aagatataac tgcagtattt cgaaatatta
    10321 tagcttaata tattattttt ctacaaattg ttctaatttt ccttcatttg tatcaaaata
    10381 aatctggcat gctgattact agccgccata tgaaagaaaa aaaccgcaaa aacgcggttc
    10441 agttctctgt ttaccatttt gcacggcttc ctttatcttc cttaatccaa ttcacattcg
    10501 ggtgctagat atacttcaat gtcactactc tttcctgctg tgccctttgc caggaaaccg
    10561 aaaagggaag caaaaatgca ttgattcaag tttctatcca gctgtcgcca tccgagattt
    10621 ttcagcttca aaagtttttt attgacactc tggagtttgt taagattctt accttgactg
    10681 gaaatattat accataaata ttgttgtata atttaaaaaa tatattataa tgggtataag
    10741 atgtttattt tgaggtgttt tgctttgtcc ggtgaattga ttacgattga tcttgaaaaa
    10801 ttgaagggct atgtcacttc aaaattagca aagtttcctg aaatacaagg cgtttactta
    10861 tttggttcca gcctggataa aatgagacct gatagtgata tagatttagg tataatattc
    10921 gatgaaaagc taagtgaaga tattgaccta ttaattgaga aagtatacta tgcgctgggc
    10981 cgctttgatg gccattcatt tgacatagtt tccgtacggg atgtaaacgc tatattatcc
    11041 tttagaattt ttcatcaagg catgccctta tacattgctg acaatgacaa agttacagat
    11101 ttgatggaaa gagtgagtag aatatactca gacatatatc cccgttatag aagagcccta
    11161 gagataataa gtggcttaag ga
//
LOCUS       DAONUO010000017        11097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4529_length_11097_cov_4.008966, whole genome shotgun sequence.
ACCESSION   DAONUO010000017 DAONUO010000000
VERSION     DAONUO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_11097_cov_4.008966"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PK768_01200"
     CDS             <1..552
                     /locus_tag="PK768_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HQA59620.1"
                     /translation="DYSEQKDKMAKEKEAVINELAREITRYLALLSRVSLSDDEYNSI
                     TDLVSTVTDIERVGDHAMNILELAEYRNDHRLPFSNIAIDELLEMSSKVQETFDLAVH
                     TFLHWDKASAQNIVKNEEEIDMMEKQLRLNHIKRLNEGSCDPSSGVIFLDLLANLERV
                     GDHSFNIASYVLNIDKNYKKLSV"
     gene            906..1607
                     /locus_tag="PK768_01205"
     CDS             906..1607
                     /locus_tag="PK768_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQA59621.1"
                     /translation="MISFYKIFFFSSISGLGVVLGGYLGTKRIPDKILAFVLAFGSGV
                     LLSVLSYSLMHEAYRLSGPVCTSIAFFLGGAFLYILEGLMTRFVAPGTGAIIGTALDD
                     LPEALSMGIGFATGQGRLGIVIALSVLLHNIPEGISSTKDLVDKAGLSSKSAMVLAVL
                     IGLLDPFAALSGYYLLRNLSEVWLGMIMAFSGGSILFMTGTSLIPKASNLGTRLENAG
                     LLVGFLAAFLISRLL"
     gene            1725..2144
                     /locus_tag="PK768_01210"
     CDS             1725..2144
                     /locus_tag="PK768_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HQA59622.1"
                     /translation="MESTDVAKIALKMALSTRSEEAELKQSFRNMGVKVCAVDFGGNL
                     VNSIEKVIERSLVAAKREGIIKENVHVLEGALIGATREALSQILQKAMGFNVGGKIGI
                     ARCGEHLTVAMFFGVGFLNFNDVVVGLGHRALPKNNS"
     gene            2319..3344
                     /locus_tag="PK768_01215"
     CDS             2319..3344
                     /locus_tag="PK768_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA59623.1"
                     /translation="MKKHFVITLMIILTLALTLAGCANSTGTDSQAEPAPQETENQEK
                     VFKIGISQFVEHPALDAARDGFIDGLREAGFEEGKNIEIILENAQADFPTTQTIANNL
                     VSENVDLILAIATPSAQSAANATKDIPILITAVTDPVEAGLAKSLEKPGTNVTGTTDM
                     NPVKEQIELLKEILPDAQNIGIIYNAGEPNSVIQVNIAKKAAEDIGLQVLEATVASSS
                     EVNQAIQSLAGRVDAIYTPTDNTVASAISAIVMAANDAKIPIIGAERGHVDGGALASL
                     GIDYYLLGKQTGHVAARVLNGENPADTPIEGSKDLKLIINKKSAEILDLDIPEPVLSR
                     ADEIIEK"
     gene            3363..4328
                     /locus_tag="PK768_01220"
     CDS             3363..4328
                     /locus_tag="PK768_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA59624.1"
                     /translation="MLQNLFTTILALVPALSSLFIKSLEQGLVFGIMALGVYITFRIL
                     RFSDLTVDGSFPLGAAVAAFLIVSGHSPFFATFIAFIAGTIAGAITGFLNTKAGISDL
                     LSGILTQTSLYSINLRIMGRPNTPLLNQPTIYSAISDVLSKIWPSFPTKYTYIIFFVL
                     FALLIKILLDSFLKTQMGFALRATGDNPQMIRSQGVDTNLSKILGLTLSNGLIALSGA
                     LVAQYQGFADVGMGVGTIVAGLASVIIGDSLFGARSVFISTLGVLFGSFLYRFSIALV
                     LSFRLAQASDLKLLTAIVVIIALTAPRFKKSLQLSTKGGKSNGNS"
     gene            4315..5109
                     /locus_tag="PK768_01225"
     CDS             4315..5109
                     /locus_tag="PK768_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA59625.1"
                     /translation="MVILENVQKIFHKNTPNENHALVDVSLKIEDGDFITVIGSNGAG
                     KSTMLNAVAGVFPIDGGKIILDGQDITNLPEYKRAAFIGRVFQDPMLGTAPSMTIEEN
                     LSMAYARPNNLALRWGLDFNRRKQFKEYLKNIPLGLENRLSTKVKLLSGGQRQALTLI
                     MATMKKPKLLLLDEHTAALDPKTALLVNSLTEEIIHKNKITTIMVTHNLEHAIKMGNR
                     LIMMHKGRIVMDIKGEEKSTMTIPRLLNKFEQLQGEKFVEDRVMLA"
     gene            complement(5106..6794)
                     /locus_tag="PK768_01230"
     CDS             complement(5106..6794)
                     /locus_tag="PK768_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HQA59626.1"
                     /translation="MKTLLVGINAKYIHTNLAIRNIAAYLNDENIIIYEATINDRLDG
                     ILEDIMSYNADIIGFSCYLWNIEEVQYLAENIKKIKPETQIVLGGPEVSYEAEDLLKV
                     KPQIDYVILGEGEERFGKLLKVFSGKMALEELDGIAYRSDGSVIVNPPKGYVYLNRIP
                     FSYSGEDLEHKLVYYETSRGCYFRCAFCLSSLETDVRFADMEKVRQDFLRFAQMGVKV
                     VKLVDRSFNCNLPRALELLEVIRELPGSTVFHCEINPELVNEDFIKALEGLEDRLQFE
                     VGIQSTNTKTLREISRTPDVKRALEGIELLKTTGIKLHVDLIAGLPHEDLESFGHSFD
                     DVYSLYPEEIQLGFLKLLKGTRLRKDAHKYGIVYRSKPPYEILYNKDISYRELCILSG
                     IARLLDKYYNTGRFRHSLNYLCSKFQRPFDLYLSFYTFCKKRGLFKLRHSLTTQYDIL
                     FDFVQSLDVDKDLFGDILKFDFMLTSSKAALPDFIKPIETKEFVNAAKEYVYNEKWLE
                     ANLPQALGLSSRNLHNQISYGLFKYDVPHGTERKEKGIVFLQKEGESYYAEFSL"
     gene            complement(6916..8403)
                     /locus_tag="PK768_01235"
     CDS             complement(6916..8403)
                     /locus_tag="PK768_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HQA59627.1"
                     /translation="MAILKINERDNVAVALDTITEGETFSVDSLSIKALEKIDKGHKI
                     ALRDIKQGENVIKYGFPIGHATTDIKAGQWVHTHNVKTNLGEISSYEYNPHLSDAEKV
                     ETDISFKGFLREDGRVGVRNEIWIIPTVGCVNRNVNMIAQIASQKYAGAKNVEGFYAY
                     THPYGCSQLGQDHLSTQTILANLVKHPNAGGVLVLGLGCENNNIDEFKKVLGEYNPDK
                     VKFLVAQEVEDEIQAGVDLIGELVEYASRFERKDCPISELVVGLKCGGSDAFSGITAN
                     PLVGSFSDKLISYGGSTILTEVPEMFGAETILMNRAINREVFEKTVKLINDFKEYFMR
                     YNQPIYENPSPGNKKGGITTLEEKSLGCTQKGGTSDVVDVLNYGDVVKEKGLSLLNGP
                     GNDIVASTVLAAAGCQIILFTTGRGTPLGTAVPTIKISTNSDLYSRKSNWMDFDAGRL
                     LAGESMEKLTEELLDYVIKVASGQKCKAEEMGFREIAIFKDGVTL"
     gene            complement(8428..9948)
                     /locus_tag="PK768_01240"
     CDS             complement(8428..9948)
                     /locus_tag="PK768_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HQA59628.1"
                     /translation="MVKLNKDLLKSGHKFPEDLQVQSYPEDLPERVIQFGEGNFLRAF
                     VDWMFHQMNKNNLFNGRVVVVQPIAQGLVDKLNEQDGLYTLFLRGLKDGKPTELKEII
                     SSVSRGINPYSQWDEYLKCAENPDIEFVISNTTEAGIAYDKSDSLDKKPPVSFPGKLT
                     AYLYHRFNHFNGDPGKGMVIIPCELIDRNGDNLRKIILQLADDWKLPEAFKEWIKEHN
                     IFLNTLVDRVVTGYPKDEIDDILAYLGYQDDLVDTGELFHLWVIEGPKELSEKLPFTK
                     VGLNVLWTEDMTPYRTRKVRILNGAHTSSVPAAFLYGLETVGEMMDHEVLGKYVRQII
                     YDEIIPSIDLDKDMLTEFADSVVERFQNPYIKHYLLSILLNSSSKFKTRVLPSILEYK
                     ELHGKLPEKLTFSLAALIAVYKDGKVEGPFMKARREKGEFTMQDDQWALEFFEDTWKD
                     YDGSKEASKKVAETVLANSKMWGQDLNEVDGLADKVADYLYQITNDGIKSTIQKLV"
     gene            complement(10008..11096)
                     /locus_tag="PK768_01245"
     CDS             complement(10008..11096)
                     /locus_tag="PK768_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQA59629.1"
                     /translation="MGIVIEVGLDKSTGGRRPINLCINPDAGYVFGVEVTRFESFVGT
                     CDLKNDPTDFRTLKIDMTEPKEGIKELAAAIKRFMNEERYKKKTFLGVGVAFPGLLNV
                     PEGIVKRSINLGHNWDTFPVKRALEEELGMHVFIENNSKASALAERWFGGGTGCKDLV
                     YVNLGEGISAGVIINDRILQGVHGHAGEIGHMSIQEAGPLCNCGNRGCLESICGIPAI
                     MRMAKAELTKIPDDDPLKKVWLGKGEFSIEDIIKSSCTKNSYSHELLKQAAVYIGKAI
                     ANVINFYNPECVFIGGKMAAAAEYSMDVIKSTVRSHAFPVIALSTDIQISSLKEKAGI
                     MGACALVLRDLFKPDSDMLEDVEQAFSK"
BASE COUNT         3060 a         2419 c         2305 g         3313 t
ORIGIN      
        1 gattacagtg aacagaagga taaaatggca aaagaaaagg aagcggttat caacgagctg
       61 gcgcgagaaa tcactcgata cctggccttg ctttcacgcg tttctttatc agatgatgag
      121 tacaattcca tcactgactt ggtaagcacg gtaaccgata tagaacgtgt tggggaccat
      181 gctatgaaca tactggaact ggccgagtat agaaatgacc atcgtctgcc ctttagcaat
      241 atagccattg atgaattgct agaaatgtct tccaaagtac aagagacctt tgatttagca
      301 gttcatacat tcctgcattg ggacaaggct tcagcacaaa atattgtaaa aaatgaggaa
      361 gaaattgaca tgatggaaaa gcagctccgt ctaaatcaca taaagcgctt aaatgaaggc
      421 tcctgtgacc caagttcagg agttatattt ctcgacctgc tggcaaattt agaacgtgtc
      481 ggggaccact cttttaacat cgcttcgtac gtgttaaaca tcgataaaaa ctacaaaaag
      541 ttatctgtat aatgctgttt atacaaaaga catttctgac gaaaatgggt cagaaatgtt
      601 ttttttattt tagggcaata taagtattgc cccataaaat gaaaaaagag cagctagttt
      661 tcttgctctt ggttcttgtt ttcttcgctg tcttcctggt ttaccttgct gttgcagtac
      721 gggcatatcc aagcacagac atctgctgcg gagtatgaac gtttattaca ataaggacat
      781 gtaaaatatg gcaatatagc ccccccctag caattctcta aaagctacgc caagctatta
      841 ttatatattc aattcgttat ttgttaaatc ctgcatgtgt aaaatatttt gtaggtgaat
      901 gagcaatgat tagtttttat aagatatttt tcttcagctc catatcaggc ctaggcgtag
      961 ttctaggcgg ctacctgggg acaaaaagaa ttcccgataa gattttagct tttgttttgg
     1021 catttggttc cggagtttta ttatcggtgc tttcctattc tctaatgcat gaagcataca
     1081 ggctttcagg accggtatgc acttctatag cattcttctt aggtggagct ttcctttata
     1141 tattagaagg gctgatgaca agattcgtcg cgccggggac aggtgccata ataggaacag
     1201 cccttgatga tttgcccgaa gctttaagta tgggaatagg ctttgccacg ggtcaaggtc
     1261 gattggggat agtgattgct ttatcggtgc tgcttcacaa tattcctgaa ggtatatcat
     1321 ccacaaagga tctcgttgac aaggcgggtc tttcatcaaa atctgccatg gtattagccg
     1381 tgctgattgg cctcttagac ccctttgcag ctctcagcgg gtattacctt ttgagaaatc
     1441 tcagcgaagt ttggttaggt atgataatgg cattttccgg cggttcaata ttgtttatga
     1501 ctggcacatc ccttatacct aaagctagta acttgggaac gcgcttggaa aatgcaggct
     1561 tgctggtggg ttttctggca gcctttctca tcagcagatt gctgtaaaat ttaatgaaac
     1621 tcccatgtgc aaagtttgat ccaatcctta acaattattt tcctcatcca aatgaatact
     1681 atgatataat aagatgagat agaatttttt cgggggtggg acttttggaa agtaccgatg
     1741 ttgccaaaat agctctaaaa atggcacttt ctacgcgctc tgaagaagcc gaactgaaac
     1801 aaagttttag aaacatgggt gttaaggttt gtgctgtgga ctttggagga aaccttgtaa
     1861 attccattga aaaagtcatt gagcgttccc ttgtcgcagc aaagcgggaa ggtatcataa
     1921 aggagaacgt tcacgtattg gagggtgcgc tgataggggc aacccgtgaa gctctttctc
     1981 agattttgca aaaagctatg ggctttaatg ttggcggtaa aattggaatt gcccgctgcg
     2041 gagagcatct gactgtagca atgtttttcg gagttgggtt tttgaatttc aacgacgtgg
     2101 ttgtaggcct tggccacaga gctttgccta aaaataattc ttaagtcaaa aatctactat
     2161 tgactttttg caactagggc tatataatac tatgcaaata aaaaaacgtg tagaacggta
     2221 gtatggtagg agatggtttg gaagaaacac tcttactggg tgtttttttg tgtacttttt
     2281 tctccccatg ctaaccaaaa agaagggggt ttttttaatt gaaaaaacac tttgtcatca
     2341 cattaatgat tatccttact ttagcactca cgttggctgg atgtgccaat tccacaggca
     2401 cggattctca ggcagaacca gctccacagg agactgagaa tcaagaaaaa gtgtttaaaa
     2461 taggcatatc ccagtttgta gaacatcccg ccttagacgc agctcgtgat ggttttatcg
     2521 acggtttaag agaagccgga tttgaagaag gaaagaatat tgaaattatc ttagaaaatg
     2581 ctcaggcgga ctttcctaca acccaaacaa ttgcaaataa tttagttagc gaaaacgttg
     2641 acttaatcct ggcaattgca acaccgtcgg cacaatcagc tgctaatgct actaaggata
     2701 tcccgatact aatcacggct gtaaccgacc ctgttgaagc aggcctcgca aaaagccttg
     2761 aaaagccggg tactaacgta acgggcacta cggacatgaa cccggttaaa gaacaaattg
     2821 agctattaaa ggaaattctg cccgatgccc aaaatatcgg cataatatat aatgctggcg
     2881 aaccaaattc ggtaatccaa gtaaacattg caaaaaaggc agccgaagat ataggtcttc
     2941 aggttcttga ggcaaccgta gcaagcagca gtgaagtaaa ccaggcaata cagtcgctgg
     3001 caggcagggt agatgccata tacacaccca cggataacac cgtagcctca gccattagcg
     3061 ctattgtaat ggctgcaaat gatgccaaaa tacccatcat cggtgccgaa cgggggcatg
     3121 tagacggcgg tgccttagct tcactcggaa tagactacta tcttttagga aaacagaccg
     3181 gtcatgtggc ggcaagggta ttaaacggtg aaaatcctgc agatacccct atagaaggat
     3241 ctaaagatct aaagttaatc ataaataaaa aatctgcaga gattctagat ttagatattc
     3301 cggaaccggt gctgtccagg gccgatgaaa ttatagagaa atagttaagg tgtgattcga
     3361 gcatgttgca aaatttattc acgacaatat tggcactagt gcccgcacta tcgagcttgt
     3421 ttataaaatc gctagagcaa ggacttgtgt tcggaataat ggctctaggt gtttatataa
     3481 cttttagaat acttaggttc tccgatttga ctgtagacgg cagtttcccc ttaggagctg
     3541 ctgtggccgc ctttctcatc gtgagcggcc acagtccgtt ttttgctaca ttcattgcct
     3601 ttattgcagg aacaatagcg ggggccatta caggcttttt aaatacaaaa gcgggaatca
     3661 gtgatttgct atccggaata ttgacccaga cttctctcta ctcaatcaac cttcgcatta
     3721 tgggtcgacc aaacaccccc ctattaaatc agcccacaat ttattctgca ataagtgatg
     3781 ttttaagcaa aatatggcct tcctttccaa ctaaatatac ttacatcatc ttttttgtat
     3841 tatttgcctt attgataaag attttgctag attctttctt aaagacacaa atgggctttg
     3901 cccttagggc taccggagat aatcctcaaa tgattagaag tcagggagtt gacacaaatc
     3961 tttccaaaat actaggtctt acattgtcaa atggtttaat tgctctgtcg ggagccttag
     4021 ttgctcaata tcaaggtttt gccgacgtag gaatgggagt aggcacaata gtggcaggcc
     4081 ttgcttcggt aatcattggt gattcacttt tcggagcaag aagcgttttt ataagcacac
     4141 taggggttct ctttggctcc tttttatacc gttttagcat tgctttggtc ctttccttcc
     4201 ggttggcaca agcctcggat ttaaaattac ttactgcaat cgtagtaatc atagcattaa
     4261 cggctccgcg ctttaaaaag tctctacagc tttccacgaa aggaggcaag tctaatggta
     4321 attcttgaaa atgttcaaaa gatttttcat aaaaatactc ccaatgaaaa tcacgccctg
     4381 gtagatgtgt ctttgaaaat tgaagatggc gattttatca ccgtcatagg cagtaacggt
     4441 gccggcaaat ccacgatgct aaatgccgtt gcaggagtgt ttcccataga cggcggtaag
     4501 atcatccttg atggccagga tattaccaat ctaccggaat acaaacgggc agcttttata
     4561 gggcgagttt ttcaggatcc catgctggga actgctcctt ctatgaccat tgaagaaaat
     4621 ctttcaatgg cctacgcgag gccaaacaat ttggccctca gatggggatt ggattttaat
     4681 cgcaggaaac aatttaaaga gtatctaaaa aacattcccc ttggcttgga aaatcgcctt
     4741 tctaccaagg taaagctgct atccggcggg cagcggcaag ccctgaccct tattatggca
     4801 accatgaaaa agccaaaact gttgctatta gacgagcaca ccgccgccct agatcctaag
     4861 acggccctct tagtaaacag tctcaccgag gaaataatac acaaaaacaa gatcaccaca
     4921 ataatggtga cccacaactt agaacacgct ataaaaatgg gcaatcggct cataatgatg
     4981 cacaagggcc ggatcgtcat ggacataaag ggagaggaaa agtcaaccat gacaattccc
     5041 cgcctcttaa ataaatttga gcagcttcag ggcgaaaaat tcgtcgaaga cagagtcatg
     5101 ctcgcttaaa gggaaaattc cgcatagtag ctttcgcctt ccttttgtaa aaaaacaatg
     5161 cccttttctt ttctttcagt gccatgcgga acatcatatt taaaaaggcc ataagatatt
     5221 tgattgtgta aattacggct tgaaagaccc aaggcttgag gcaggtttgc ttcaagccat
     5281 ttttcattgt aaacatattc tttagccgca tttacaaatt ccttagtttc aatcggtttt
     5341 atgaaatccg gcagggctgc cttactgcta gtgagcatga aatcaaattt tagtatatcg
     5401 ccgaaaaggt ctttatccac gtcaaggctt tggacaaaat caaaaagtat gtcatattgg
     5461 gttgttagtg aatgccgtag cttgaaaagc cctctttttt tgcagaacgt gtaaaaagat
     5521 agatataagt caaaaggcct ttggaattta ctgcagaggt aatttaagga atgcctgaaa
     5581 cgaccggtat tgtagtattt atctaggagc cttgcaatac cggaaaggat gcaaagctcc
     5641 cggtaactta tgtctttgtt atataaaatc tcatagggag gctttgaccg atatacgatg
     5701 ccgtatttgt gggcatcttt tcttaacctt gtccctttta agagctttaa aaatcccagc
     5761 tgtatttcct cagggtagag actatacaca tcatcaaaag aatggccaaa actttctaaa
     5821 tcctcatggg gcaaacctgc tattaaatct acatgaagtt taataccggt tgttttaagt
     5881 agctcaatgc cctccagcgc ccttttcacg tcgggagtcc tggatatttc ccttagagtt
     5941 ttagtgttgg tactttgaat acctacttca aactggagcc ggtcctctag accttccagg
     6001 gctttaataa aatcctcgtt taccagctcg ggatttattt cgcagtgaaa gaccgtactg
     6061 ccgggcagtt cccgtatgac ttccagtaat tctagtgccc ttggcaggtt acagttaaag
     6121 gagcggtcga ctaattttac aactttgact cccatttggg caaaacgcag gaaatcctgc
     6181 ctcacctttt ccatatcggc aaatctgaca tcggtttcca gtgaagatag gcaaaaggcg
     6241 catctaaaat agcaacccct agatgtctca tagtacacta gtttatgttc caaatcttca
     6301 ccgctatagg aaaaaggaat gcggttcaag tatacgtagc ctttcggtgg attaacaatt
     6361 acacttccat cgcttcgata agctatccca tctaattctt ccaaagccat cttgccgcta
     6421 aatactttga gcagcttgcc gaaccgctcc tcgccttcac ccagtatcac atagtcaatt
     6481 tgcggcttca cttttagcaa atcttccgct tcgtaagaaa cttctggccc acccaagaca
     6541 atttgcgttt cgggctttat tttttttata ttttcggcta aatattgtac ttcttcaatg
     6601 ttccataaat agcaggaaaa gccgattata tcagcattgt aagacatgat gtcttctaaa
     6661 atcccgtcca atctgtcatt tatggtagct tcataaatga tgatattttc atcatttaaa
     6721 tatgctgcaa tatttcttat ggccagattt gtgtggatat acttggcatt tattcctact
     6781 aacaaagtct tcatatacat ccctcggctt gttttttaca attattatac catcctttta
     6841 ctgctactac acacaataaa aaaatcaccc tgactaagct tttaagctaa ggccaggatg
     6901 atttaaaggg cgaatttata gggttacgcc atccttgaag atggcaattt ctctaaatcc
     6961 catttcttca gccttgcact tttgacctga agcgactttt atgacgtaat ctaatagctc
     7021 ttcagttagt ttttccattg attcgccagc aaggagccta ccagcatcaa aatccatcca
     7081 gttggatttt ctgctataaa ggtcgctgtt ggtggagatt tttattgtag gcaccgctgt
     7141 ccccaggggt gtgccacgac ctgtagtaaa taggattatt tggcaacctg cagctgctag
     7201 gactgtggat gctactatgt cattgccggg accgtttaac aggcttaggc ccttctcttt
     7261 aacaacatca ccgtaattga ggacatctac gacatcactt gttccgccct tttgggtgca
     7321 gcccaaggat ttttcttcca aggttgtgat accacccttt ttgttgccgg gagatgggtt
     7381 ttcatagatg ggttgattgt acctcatgaa atattcttta aagtcattta tgagttttac
     7441 cgtcttttca aacacttccc ggtttattgc ccgattcatt agaatggttt cagcgccgaa
     7501 catctcgggc acttccgtca aaatagtgct cccaccgtag gagatcaatt tatcggaaaa
     7561 ggaacctact agggggtttg cagttatgcc ggaaaaagcg tcagaacctc cgcattttag
     7621 gccaactact agttcggata tagggcagtc ttttctttca aaccgtgatg cgtactccac
     7681 caattcacct ataagatcta ctcctgcctg aatttcatct tctacttcct gggccactaa
     7741 aaactttacc ttatccggat tgtattcgcc caaaaccttt ttaaattcat cgatgttgtt
     7801 gttttcacag ccaaggccca agaccaaaac tcctccggca ttagggtgct tgaccaaatt
     7861 tgccaaaatg gtttgagtgc taagatggtc ctgacctagc tgggagcaac cgtaagggtg
     7921 agtatatgca taaaagccct caacattttt tgcgccggca tacttttggg aagcaatctg
     7981 ggcaatcata ttgacgtttc tgttgacaca gccgaccgtt ggaattatcc atatttcgtt
     8041 tcgcacacca actcggccgt cttcgcggag aaagccttta aagctaatgt cggtttcgac
     8101 cttttcagca tcagaaagat gggggttgta ttcatagctt gatatctcgc ccaaatttgt
     8161 tttgacattg tgagtgtgaa cccattgacc ggctttaata tcagtcgtag cgtggccgat
     8221 gggaaagcca tattttatta cattttcacc ctgtttaatg tcgcgaaggg caattttgtg
     8281 gcccttatcg attttttcca aggctttgat cgaaagggaa tcaaccgaaa atgtttcacc
     8341 ctcagttatg gtatcaaggg ctactgccac attgtccctt tcattaatct tcaatatcgc
     8401 cataaaaccc ctcctctcgg gctgtcttta taccagcttt tggatagttg actttatgcc
     8461 gtcatttgta atttggtata ggtaatcggc aactttgtcc gcaaggccgt caacttcatt
     8521 taggtcttgg ccccacatct tgctatttgc aaggacggtt tcggctacct tcttggaagc
     8581 ttctttgctg ccatcataat ccttccaggt atcctcgaaa aattccaaag cccactgatc
     8641 atcttgcatg gtaaattcgc ctttttccct gcgggctttc atgaaaggcc cttctacctt
     8701 cccgtccttg taaactgcaa ttaaagctgc cagggagaat gtcagctttt caggcaattt
     8761 gccatgtagt tccttgtact caagaatcga cggcaagacc cttgtcttga atttagatga
     8821 agagttgaga agtatgctta atagatagtg cttaatatag ggattttgga agcgttccac
     8881 tactgagtct gcaaactcgg tcagcatgtc tttgtccaag tctattgaag gaattatctc
     8941 atcatagata atctgacgca catatttgcc caggacttca tggtccatca tctcgcccac
     9001 tgtttctaaa ccgtacagga aggctgcagg taccgaggaa gtgtgagcac cgtttaaaat
     9061 acgaactttc cgggtcctgt agggagtcat atcctcggtc cagagcacat ttagaccgac
     9121 tttggtaaag ggaagtttct ctgaaagttc ttttggcccc tcgattaccc acaggtggaa
     9181 aagttcacct gtgtccacca ggtcatcctg atagcccagg tacgccaaga tgtcgtcgat
     9241 ttcatccttg ggatagccgg tgaccactct atcaaccagc gtatttagga aaatgttgtg
     9301 ctccttaatc cactccttaa atgcttcagg caacttccag tcatctgcca gctgtaagat
     9361 gatttttctt aaattgtcgc cgtttctatc gattagctca cagggtatta ttaccatacc
     9421 cttgccggga tctccattga aatgattgaa tctatggtat agataggctg taagctttcc
     9481 ggggaaggat acgggtggtt ttttgtccaa gctgtcgctc ttgtcatatg ctatccctgc
     9541 ttcagtagta ttggaaatta caaactcaat atcgggattt tcggcacatt taaggtattc
     9601 gtcccattgg ctgtatggat ttatgccgcg gctgacagaa ctgatgattt cttttaattc
     9661 agttggtttg ccatccttta atcctcttag aaataaggta tacaggccgt cttgttcgtt
     9721 tagcttgtca acgaggccct gcgctatggg ctggaccact acaacgcggc cgttaaagag
     9781 attgttcttg ttcatctgat ggaacatcca gtcaacaaaa gcccttaaga aattgccctc
     9841 gccaaactgg attactcttt ccggcaggtc ttcgggataa gactgcacct gtaaatcttc
     9901 aggaaactta tgaccgcttt ttaataagtc cttgtttaac tttaccataa gcatacctcc
     9961 atttttttat tctaatgaga acccttgatg gttgagcttt aaaaacttca ttttgaaaaa
    10021 gcttgctcca catcttctaa catgtcggag tcaggcttaa acaaatcccg caaaaccagt
    10081 gcacacgcac ccattatccc ggctttttct ttaagtgagg atatttgtat atcggtagaa
    10141 agggctatta cagggaaggc atggctcctt acggtgcttt ttattacatc catggagtat
    10201 tcggctgctg cagccatttt accaccgatg aatacgcact cgggattgta aaagtttatg
    10261 acatttgcta tggctttacc gatatatacg gcagcctgtt ttaacagctc gtgggaatag
    10321 gaattttttg tgcaggatga tttgattata tcttcgatgg aaaattcgcc ttttcccagc
    10381 cataccttct ttaaagggtc atcatcgggt attttggtaa gttctgcctt ggccatcctc
    10441 attatggctg ggataccgca gatactttca aggcatccgc gatttccgca gttgcacagt
    10501 ggaccggctt cctggatcga catgtgaccg atttcaccgg catggccatg aactccctgt
    10561 aagatgcgat catttatgat gacaccggcg ctgataccct cacctaaatt cacgtacacc
    10621 aaatccttgc atccggttcc tcctccaaac cacctctccg ccagggcaga tgctttagaa
    10681 ttgttctcta tgaaaacatg cattccgagt tcctcttcta aagcccgctt gacgggaaaa
    10741 gtgtcccagt tgtggcctag atttatagag cgcttgacaa tgccctcagg cacatttaac
    10801 aagccaggaa aagctacccc aactcccaaa aaagtctttt tcttatacct ctcttcattc
    10861 atgaaccgtt ttattgcagc ggccaactct ttgatgcctt cttttggctc tgtcatatcg
    10921 attttaagag tacgaaaatc ggttgggtca ttttttaaat cacaagttcc cacaaaggat
    10981 tcaaaccttg taacctccac cccaaaaaca tatccggcat cgggattgat gcagagattt
    11041 atgggccttc tgcctcctgt ggatttgtca aggccaactt ctatcacaat gcccatg
//
LOCUS       DAONUO010000018        11071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4543_length_11071_cov_3.564542, whole genome shotgun sequence.
ACCESSION   DAONUO010000018 DAONUO010000000
VERSION     DAONUO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11071
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4543_length_11071_cov_3.564542"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PK768_01250"
     CDS             <1..569
                     /locus_tag="PK768_01250"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HQA59630.1"
                     /translation="RCRKCGTKISVMYPLVELLTGLMFIFAFIKFGLTFEFLSAIILI
                     TCLMITTFIDLEHQIIPDKVVLPSLAAGLVLNIIFHREDFYNYLLGFALGGGIIFLIV
                     VLSRGGMGGGDIKLFATVGMFLGFRLTLLALLLSFVLGSVVGLILIILKLKNMKDAIP
                     FGPFIALASVISMLAGDRIISWYWGLFF"
     gene            581..1039
                     /locus_tag="PK768_01255"
     CDS             581..1039
                     /locus_tag="PK768_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQA59631.1"
                     /translation="MHKKGYTLIELILVISIIGILATISLPIYRTSLAHYRHSMALRM
                     IVSDIRMIQQQSITERRSYKLIFNTTETTGHLTDYWLGREESIVESRQLPEGNRVIET
                     NFENDTLSFNPSGEPSRGGGHITIGDSLGNKSYVIVTPATGKVKISDKLS"
     gene            1023..1436
                     /locus_tag="PK768_01260"
     CDS             1023..1436
                     /locus_tag="PK768_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQA59632.1"
                     /translation="MISFLKGAGDQRGFTLIELIVAMTILAIIIAPTLGLFSATFKNN
                     RHTKEKLVALALAQGEIEELKAMDFQRLLQRVGESTKKLPAEDGTVYTVHREIELLDD
                     EDDEPKLLKITVTVSWTGGEVSFSTVKGDIDYGEI"
     gene            1423..1902
                     /locus_tag="PK768_01265"
     CDS             1423..1902
                     /locus_tag="PK768_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQA59633.1"
                     /translation="MGKFKESSGMTLIELIVSLGIMVMVLSAVFSFYSAGMKSWLISE
                     NQMEVLQNARIALEWISRDLKVASEYEILSRQEISITSYQGKIIYEHDDKEKQLIMKK
                     NGDIMPVADYIVDLRFEEDSSGAIVIIELTAESNSYEVKLSTKIAPPVMKTPETEET"
     gene            1923..3191
                     /locus_tag="PK768_01270"
     CDS             1923..3191
                     /locus_tag="PK768_01270"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQA59634.1"
                     /translation="MSCIKANQGLVLIVLLFTVAVISVLGATIVSASLRENMIAYYQE
                     DYVSAQYIAECGLQKALVSLEEHPDWESDDYWNDLLGREIEFEDGSYKLEFSKKGSYE
                     PGNELEVVSAGKYRSAVCVIKADVKITKVHTKIYDNLVSINSETAITYSGNADIHGDM
                     YCNTDMTLNGNAYVSGNVTNLGKSTLGGSSEIGGDLYSEGDIELFGDATIRGNVKSRG
                     NLTLKGNSKIKGTIQVNGVVTPEGKYDFIHGGVEAREPMVFPKLDELLDTYLQEAQKE
                     GKYYSYGNIPSKISGTTFVDSNVSISGNKDITGHGVLVINGNLDMSGNIDIISEDGGA
                     VVIIVNGDVIIGGQKSLKCVFFTAGNFDVKGNTDIYGCIVSKNITGSGNLDIWPLANL
                     ENRLPDNAPGIALGEFETKVELVALTYENE"
     gene            3266..4309
                     /gene="pilM"
                     /locus_tag="PK768_01275"
     CDS             3266..4309
                     /gene="pilM"
                     /locus_tag="PK768_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HQA59635.1"
                     /translation="MFWGNNNSILGLDIGSSHIKIVHLKRYRQNWKLENRAIVKTPTD
                     AISDGNISNKDELVRVLTDTIAKHKFQERKVVTAVSNMRIVTRYITMPPMPEKELKEA
                     VNWEAKNHIPIYDENMKIDFRLLGKTDDNRTRLVIAGISKQTASEYLDMLTDARLKPV
                     AIDIYPVSLQRFFGGSDSSRPYSIVDLGAVSTKLVIIKEDSVYADSLIAVGIKDIQRN
                     IIDYFGIQGEELASWQESFSLRSLRDKQEHAELFRILAPSLNELTDGINRFIKFFDTQ
                     NRNQTVAKLVLTGGGALWKGIGDFIGQETGIPTSTVEPELTNPTLDTSTLGREELHLL
                     SNAVGLAMRGVVY"
     gene            4309..4866
                     /locus_tag="PK768_01280"
     CDS             4309..4866
                     /locus_tag="PK768_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQA59636.1"
                     /translation="MNEINLLPDEYIKTARKKPKRTLVMGLSLAVVALLILLSASFIG
                     RYQEIRTAIDHINELYNESFRPLLEREKELRATEREISEKYSLYLELEEHKTIMSEIL
                     LEIAQLTPDNVQIVYIDVGNDKNAVIRGRAPSDRVVANFMASLGKSENLLNINLDHIT
                     SEAGRGDDGLFTFQLTFFLKEKGEM"
     gene            4868..5479
                     /gene="pilO"
                     /locus_tag="PK768_01285"
     CDS             4868..5479
                     /gene="pilO"
                     /locus_tag="PK768_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HQA59637.1"
                     /translation="MIQLNNREKRFIIIGIIIILVSLAYARLIYPTYIKLNALMKEYK
                     EKQGIEGTLEEMESRVEILEAKIDDDMKLLEKVQQAIPYGKDIHKLLLDLEAFIEKHS
                     LRQHAFAPQELEDRESHYILPINIRVSGEFEDIIGFLKDWESYHRIVNITAAKLYPEE
                     GSTIVGEFVANIYILKAEDGLVEDYEFPGFDGDNGRSDPFAPF"
     gene            5504..6040
                     /gene="ilvN"
                     /locus_tag="PK768_01290"
     CDS             5504..6040
                     /gene="ilvN"
                     /locus_tag="PK768_01290"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQA59638.1"
                     /translation="MRRAVFSVLVHNEYGVVTRISGLFTRRGFNIKSFTGEETEDPKI
                     SRITIVAEGDERELAQIKSQVEKLVDVIKVVELDVKASVQRELALIKVRTQDKNRTEI
                     FQIVETFRGNIIDIEPGSVIIELTGDASKVKAFLNIMKIYGIIEIVRTGAIALERGRE
                     SIYDAPDPDRTPVEEESE"
     gene            6037..7695
                     /gene="ilvD"
                     /locus_tag="PK768_01295"
     CDS             6037..7695
                     /gene="ilvD"
                     /locus_tag="PK768_01295"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQA59639.1"
                     /translation="MISDRVKKGIMRAPHRSLFYAMGYTKEELDRPLIGIVNSQNEIV
                     PGHFHLDTIAQAAKIGVAMAGGTPMEFPAIGVCDGIVMGHIGMKYSLASRELIADSIE
                     AVAVAHGFDGLVLIPNCDKIVPAMLMAAARLNIPAVVVSGGPMYAGRHKGRDIDLNTC
                     FEAVSAFSSGKLTAEELEEIEQAACPGCGSCSGIFTANSMNCLTEVLGMGLPGNGTIP
                     ATSGARIALAKKAGMQVMEMVRRGIKPRDIMTREAFENAITVDMGMAGSTNTVLHLPA
                     IANEAGIKLELDIFDEISSRTPYLAKMSPSGEHHVEDLHEAGGIPALMKELSRKDLLH
                     LDLITVTGKTVGENIANVRIKNTEVIHTIDNPYRETGGLAILKGNLAPDGAVVKEAAV
                     APEMLYHEGPAKVFESEEEVTEAIYSGKVEKGDVLVIRYEGPKGGPGMREMLSPTSSI
                     AGMGLDKDVALITDGRFSGATRGASIGHVSPEAMEGGPIALIQNGDIIRIDIKNRKLD
                     VLLSEEELSRRRANWQKPEPKVKSGYLSRYARLVTSASTGAVLR"
     gene            complement(7767..8144)
                     /locus_tag="PK768_01300"
     CDS             complement(7767..8144)
                     /locus_tag="PK768_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQA59640.1"
                     /translation="MIIYIVQPGDTLSVIANRFNVTIQDIIENNWVGTDMMIVPGQIL
                     FIPRDRHHKYDSMDSADPLSMLQPYIKQQVLYVVQRGDTLAAIARRFDSTVEAIAEAN
                     RFDGANVPIFPGQILRVPIIGFR"
     gene            8400..9467
                     /locus_tag="PK768_01305"
     CDS             8400..9467
                     /locus_tag="PK768_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQA59641.1"
                     /translation="MRKSKRVTIYDIAKKINASPSTVSRVLSNSDYPVSPEMRKKVLK
                     AAKEMNYYPNILARNLKTKNSKNVGIVLPSISNPFYPSVVRGIEDVALKNDYTLFICS
                     CEGNKDREKKYFQYLMENLSIGIITIFTDLYTEGIQEFIEHGGVVVSVCAERVDNSGV
                     CCIYFDKEKEGYMAAKHLIDLGHENIAILTPPLTNPLRIGKVEGYKRALIEAGIAINQ
                     DYIYIASEEYAPNYMDTIYECNIASQLTRDLLQRTPEVTGIICINDIMALGCIAELES
                     HGYRVPEDYSVVGFDDLFLSNLVKPRLTTVRFEKYNVGRLAMEILQKIVEGEQRIKQY
                     NFSKYTYLEKRESTGRPRVKL"
     gene            9629..10654
                     /locus_tag="PK768_01310"
     CDS             9629..10654
                     /locus_tag="PK768_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HQA59642.1"
                     /translation="MKKVLKVLLAATLVVALLAGCGSGGTGGSGGGSQEAQPVKLIAA
                     HVNPEDSSFHAGMVAFKEKLEELSGGSMTVEIHHSGQLGGNENELVEKMATGTVDVIV
                     SAPNFVAAIVPEADLFSIPYLYEDVEHWERVVTGEVGQTILDKIEAGTDFKPLGYWMC
                     GVRQYFGKKPITTPDDLKGVKIRVHNSETIQSIWSALGAQPAILAYNELYSGLQNNVI
                     DAAENDFGSILQMKFYEVGPYISLTNHDIATRYFLMSKAKYNSLTDEQKAWIDEAAKA
                     SCEHQWVFDRNLIEEAKVKLEEAGVKFNETEMHLFIDKTQEVREKAAEKLGVSDLLEK
                     ISELRDN"
     gene            10815..>11071
                     /locus_tag="PK768_01315"
     CDS             10815..>11071
                     /locus_tag="PK768_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HQA59643.1"
                     /translation="MKEALSKLERILVYVGNACFVLFFAMTTIQVTARAVGFNSAYTE
                     EIANYAFIWLAFVGSSICIRSDEHFRFTAFTERLSEKGKLI"
BASE COUNT         3460 a         2131 c         2629 g         2851 t
ORIGIN      
        1 gaaggtgcag gaagtgtggg actaaaattt cggtaatgta tccgctggtg gaacttttaa
       61 ctggtctcat gtttattttt gcctttatta aatttggctt gacctttgaa tttttgtccg
      121 caatcatatt gattacttgc ctcatgatta ccacctttat cgatttagag caccaaataa
      181 tacccgataa agtagtttta cctagccttg ctgccggttt agtgcttaat attatctttc
      241 acagggaaga tttctataac tatttattgg gttttgcatt gggtggaggc attatttttc
      301 ttatagtggt cctttcccgg ggcgggatgg gaggcgggga tataaagctt ttcgcaactg
      361 taggaatgtt tttgggtttt aggctcaccc tcttggcatt gcttctctcc tttgtattag
      421 gcagtgtagt cgggcttatt cttatcatac tgaaacttaa aaacatgaag gacgccattc
      481 ccttcgggcc ttttattgct ctggccagcg ttataagcat gcttgctggc gataggatta
      541 tttcttggta ttggggattg ttcttttagg aggggaaata ttgcacaaaa agggttatac
      601 gttaattgaa cttattctcg ttatatcaat tatcggaatt ttagcaacta tttccctgcc
      661 gatatacaga actagcttag ctcattaccg gcattcgatg gctttacgca tgatcgtatc
      721 tgacatacgt atgattcaac agcaatccat aacagaaaga cgttcataca agctgatctt
      781 taacacgacc gaaaccaccg gtcatttgac cgattattgg ttgggccgtg aagaaagtat
      841 agtagagtca aggcagctac ctgaaggcaa tcgtgtcatt gagacaaact ttgaaaatga
      901 tacactgagt ttcaatccat caggggaacc ctcccgagga ggaggccata ttactatcgg
      961 cgacagctta gggaataaaa gctatgttat tgttacaccg gctaccggga aggtgaagat
     1021 aagtgataag ctttcttaaa ggggcaggtg accaaagggg atttacactg attgaattaa
     1081 tcgtggcgat gacaatactt gctattatca tagctccaac tttgggcctt ttttccgcca
     1141 cttttaaaaa taatcgccat acaaaggaaa aacttgtagc tctagcactt gcccaaggtg
     1201 agattgaaga gctaaaggcc atggatttcc aaaggctttt gcaaagggta ggagaatcaa
     1261 caaagaagct gcctgcggaa gacgggaccg tatataccgt gcatagggaa attgagttgc
     1321 tggatgatga agatgatgaa cccaaactgt tgaagataac ggtaaccgtc tcttggactg
     1381 gtggagaagt aagtttttcc accgttaaag gggatataga ttatggggaa atttaaagaa
     1441 agctcgggaa tgacactcat tgaacttatc gtatccctgg gaatcatggt aatggtactt
     1501 tcagcggtat tcagttttta ttctgccgga atgaaatcct ggttgatttc cgaaaaccaa
     1561 atggaggtcc tgcaaaatgc aagaattgcc ttggaatgga tctcccgtga cttaaaggtg
     1621 gccagtgaat atgagatctt atcccgtcaa gaaataagta ttacatcata tcaaggcaaa
     1681 attatttatg aacacgatga taaagaaaag caactgatta tgaaaaaaaa cggcgacata
     1741 atgccggttg cagattatat agttgatttg cgctttgaag aagacagctc cggtgcaatt
     1801 gtaataatag agttgacggc agaaagcaac tcttacgaag tcaaactatc tacaaagata
     1861 gcaccaccag tgatgaaaac accggagaca gaggaaactt gaaaccggaa aggaaggaga
     1921 ctatgagctg tattaaagcc aatcagggtt tagttttgat agtgctgctc tttacagtgg
     1981 cagttatatc agttcttggt gccacgatag tttctgcgtc ccttcgagaa aatatgatag
     2041 catactacca ggaggattac gtatcagccc agtacatagc tgagtgtggc ctccaaaagg
     2101 ccttggtgtc attggaagaa caccctgatt gggaaagcga tgattattgg aatgaccttt
     2161 tgggccggga aattgaattt gaagacggaa gctacaagtt ggaatttagt aaaaaaggca
     2221 gctacgaacc gggaaatgaa cttgaagttg tttcagcggg caaataccgg tctgcggtgt
     2281 gcgtaatcaa agctgatgtc aagataacca aagtgcatac caaaatctat gataacctgg
     2341 tatcaattaa cagcgaaaca gccattactt acagtggaaa tgcagatatt catggagata
     2401 tgtattgtaa cacggatatg actcttaatg gaaatgcata cgttagcggt aatgttacaa
     2461 atttagggaa aagcacatta ggtgggagtt ctgagatcgg cggggatctt tattcagaag
     2521 gtgatataga gctgtttggg gatgctacaa taagaggaaa tgttaagagc aggggcaact
     2581 tgactttaaa aggaaattcc aaaataaaag gtacaattca agttaatggt gtagttactc
     2641 cggaaggcaa gtatgatttt atccacggtg gagtggaggc acgtgagcct atggtatttc
     2701 cgaagctgga cgaattatta gatacttacc tccaggaggc acagaaagaa ggcaagtatt
     2761 attcttatgg caatatacca tcgaaaattt cagggacaac ttttgtggat agcaatgtaa
     2821 gtattagtgg aaataaagat atcacgggtc atggggtcct tgtgataaac ggaaatcttg
     2881 atatgagcgg aaatatcgat attatcagtg aagatggcgg agcagtggtt attatagtaa
     2941 acggtgatgt aataattggg ggacaaaagt cattaaaatg tgtgtttttc accgcgggaa
     3001 attttgatgt taaaggaaat actgacatct atggctgtat tgtatcaaag aatattaccg
     3061 gttcaggcaa tcttgatatc tggccactgg ccaatctgga aaacaggctt cccgataatg
     3121 cacctggaat tgcactggga gagtttgaaa caaaagttga acttgtggct ttaacctatg
     3181 aaaatgaata aacatcaact tagttttcat atacaacaat tcagataaag gaaaaataat
     3241 attattgttg taaggggggc aaaaattgtt ctggggcaat aacaattcca ttcttgggct
     3301 tgacataggt tcttcccata taaaaattgt ccatctgaaa cgatacaggc aaaactggaa
     3361 acttgaaaac cgggccattg tcaaaacccc cacagatgct atttcagacg ggaacatatc
     3421 caataaagat gaactggtca gggttttaac tgacactatt gcaaaacaca agttccagga
     3481 gagaaaagtt gtaactgctg tgtccaatat gaggattgtc acccgctata tcaccatgcc
     3541 tcccatgccc gaaaaggagc tgaaagaagc cgtaaattgg gaggccaaaa atcacattcc
     3601 catttatgac gaaaacatga agatagactt caggctgctg gggaaaaccg atgacaacag
     3661 gacgagactt gtaatagcgg ggatatcaaa gcaaactgct tccgaatacc tggacatgtt
     3721 gactgatgcc aggctaaaac ccgttgccat agatatttat cctgtttccc tgcagagatt
     3781 ttttggaggc tccgacagca gccggcctta tagcattgtg gatttggggg cagtcagcac
     3841 aaaactcgtg ataatcaaag aggattccgt ttatgcggac agccttattg ccgtaggcat
     3901 aaaggatatt cagagaaaca taattgacta tttcggcatc caaggggagg aacttgcctc
     3961 ttggcaggag agcttttcac ttagatcttt aagggataag caagagcatg cagagctctt
     4021 tcgaattctt gcgccttccc tcaatgaact aacggacggc ataaacaggt ttataaagtt
     4081 cttcgataca caaaatcgaa atcagaccgt tgctaagctg gtattgacgg gaggcggcgc
     4141 cttgtggaaa ggaattggcg attttatcgg tcaggaaaca ggcattccta ctagcacagt
     4201 agaacccgaa ttaacaaatc caacccttga tacttcaacg ctcggcagag aagagctgca
     4261 tcttttatcc aatgccgtag ggcttgcaat gaggggagtg gtctattgat gaatgaaata
     4321 aatctcctcc ccgatgaata cataaaaaca gcaaggaaaa agcccaagag aacgcttgta
     4381 atgggccttt ccctggccgt ggtggccctt ttaattttgc tttccgcaag cttcataggt
     4441 aggtaccagg agataaggac tgcgatagac cacataaatg agctttacaa tgagagcttc
     4501 agaccccttt tggaaagaga aaaagagctt agggcaaccg aaagggagat aagcgaaaag
     4561 tacagtttat acttggagtt ggaagagcat aagacaataa tgtcagagat acttctcgaa
     4621 attgcacagc ttacacctga caacgttcag attgtatata ttgatgttgg caatgataaa
     4681 aacgctgtca ttagaggacg ggcaccttct gatagggttg ttgcgaactt tatggcaagc
     4741 ttaggcaagt cggaaaacct tctaaacata aatctcgacc acatcacaag tgaggctggc
     4801 cggggagacg acggtctttt cacattccaa ttaacatttt tcttaaaaga aaaaggtgag
     4861 atgtgaaatg attcaattga ataatcggga aaaaaggttt attattatag gtatcatcat
     4921 tattcttgtt tccctggcat atgcccggct tatttatcca acgtacatca agctcaatgc
     4981 cctgatgaaa gagtacaagg aaaaacaggg aattgaaggg accttggaag aaatggagtc
     5041 cagggtagaa atcctcgagg caaaaataga cgacgacatg aaattgcttg aaaaggttca
     5101 gcaggccata ccatacggaa aggacattca caagttgctt ctagacttag aagcctttat
     5161 tgaaaagcac agcctaaggc agcatgcctt tgctccccag gagctggagg acagggaaag
     5221 ccattacatc ctgcccatta atatcagggt ttccggtgag tttgaggata ttataggatt
     5281 tttaaaggat tgggaaagtt atcatagaat tgtcaacata accgcagcca agctgtatcc
     5341 ggaagaaggc agcactatag tcggtgaatt tgttgcaaat atatatatac tcaaggctga
     5401 agacggcctt gttgaagatt acgaatttcc cggctttgac ggcgataatg gcaggagcga
     5461 tccctttgct cctttttaat cacaaaagaa ggaaggtgtt agtgtgcgca gagctgtttt
     5521 ctcggtactt gtccacaacg aatacggtgt ggttacccgt atttcggggc tatttaccag
     5581 gaggggtttt aatatcaaga gctttaccgg cgaagaaacg gaagacccca agatatcccg
     5641 catcaccatt gtagctgaag gcgatgagcg ggagcttgcc caaataaaaa gccaggtgga
     5701 aaaattagta gatgtgataa aagttgtgga acttgacgta aaagcttcgg tgcagcggga
     5761 actggccctt atcaaagtcc ggacacagga taagaaccga acggagattt ttcaaattgt
     5821 agaaacattt cgaggcaata tcatagacat cgaacccggg tccgtaataa ttgagcttac
     5881 gggagatgcc agcaaagtca aggcattttt aaatataatg aagatctacg gtatcatcga
     5941 gatagtgagg acgggggcca tagccctgga gcgaggcagg gagagcatat acgatgcgcc
     6001 ggacccggac agaacaccgg ttgaggagga aagcgaatga taagtgacag agttaaaaaa
     6061 ggtatcatga gagcacccca cagatccctt ttttacgcta tggggtacac aaaagaagaa
     6121 ctggacagac cccttatcgg catagtaaat tcccagaatg aaatagtccc cggccacttt
     6181 cacctggaca ccatagccca ggcagccaaa ataggtgtgg ccatggcggg gggcacgccc
     6241 atggagttcc cggcgattgg cgtctgcgac ggcatcgtaa tgggccacat cggcatgaag
     6301 tattccctgg cgagcaggga gcttatagcc gattccatag aagcggtagc ggtagctcac
     6361 ggctttgacg gcctggtgct gatccccaac tgtgacaaga tagtgcctgc catgctgatg
     6421 gcggcagcca ggcttaacat acctgcggta gtggtaagtg gcggccccat gtacgcgggc
     6481 agacacaagg gcagggacat cgatttaaac acctgttttg aagcagtttc ggctttttca
     6541 tccggcaagc taactgccga ggagcttgaa gaaatagaac aggctgcatg tcccggatgc
     6601 ggctcgtgtt ccggtatttt cactgcaaac tccatgaact gcctgaccga ggtcttaggc
     6661 atgggccttc cgggcaacgg caccatacct gcaacaagcg gtgcccgtat agcactggcc
     6721 aagaaagccg gcatgcaggt catggagatg gtaaggcgcg gcattaagcc tcgagatatc
     6781 atgacgaggg aagcttttga aaatgccata accgtggata tgggcatggc aggctccacc
     6841 aatacggtgc tgcacctgcc ggcaattgcc aatgaagccg gcatcaagct ggagctggac
     6901 atctttgatg aaataagcag caggactcct tacctggcca aaatgagtcc cagcggcgag
     6961 caccacgtcg aggaccttca cgaggccgga ggaattcccg ctctcatgaa ggagctttcc
     7021 agaaaagact tgttgcacct tgacctgatt accgtaacgg gcaagacggt aggtgaaaac
     7081 attgcaaatg tccgtatcaa aaacaccgag gtaattcata ctatcgacaa tccctacagg
     7141 gaaacgggtg gccttgccat cttaaagggc aatctggccc ccgacggtgc ggtagttaag
     7201 gaagcagcgg tagcgccgga aatgttgtac cacgaaggcc ccgccaaagt ctttgaatct
     7261 gaagaagagg tcacggaagc catttattcc ggcaaggttg agaaaggcga tgtcttggtc
     7321 atccgctatg aaggtcccaa gggagggccg ggcatgcggg aaatgctttc ccccacctct
     7381 tctatagcgg gcatgggtct tgataaggac gtggccctaa ttaccgatgg ccgcttttcc
     7441 ggggcaacgc ggggtgcctc gataggccat gtgtcccccg aagccatgga agggggtccc
     7501 atagccttaa tccaaaatgg tgatataata agaattgaca taaagaacag gaagctggac
     7561 gtgctccttt cggaggaaga attgtcaagg cgaagggcca attggcaaaa gcctgaacct
     7621 aaagtcaaaa gcggctatct ttcccgatat gcacgccttg taacgtcagc atcaactgga
     7681 gcggtgctaa gatagcaact tatagtatcc tgagctttca acaagaaacc ccctgatttt
     7741 ttgctaaaag atcagggggc ttttaattat ctgaatccga taatgggaac cctgaggatt
     7801 tgacccggaa atatggggac atttgctcca tcaaatcgat tggcctcagc tatggcctcc
     7861 actgtgctgt caaacctgcg ggcaatagct gccaaagtgt caccccgctg caccacataa
     7921 agcacctgct gcttgatgta cggttgcagc attgataaag gatctgcaga atccattgag
     7981 tcgtacttgt gatgacgatc tcggggaata aacaggatct ggcccggaac aatcatcata
     8041 tcggttccta cccaattgtt ttcaattatg tcctgaattg taacattaaa cctattggct
     8101 ataacgctga gggtgtcgcc gggctgaacg atgtatatta tcatagcacc atcctcctct
     8161 ttatacttta atagtatttt attcataccg ccgataaaag tttacttcga aaattcaacc
     8221 tggcgaaaaa tcatcattat caatcttgta aatatttcca gaaaataatt ttgtacaaaa
     8281 agaggatttt ctaaaaatgc ctcgaatata taatagtatg aaatatagaa atcggttgct
     8341 caatcgattt ctattcgatt tgtaaaccaa attgcgatta ttgtgaagat gtgattataa
     8401 tgagaaaatc aaagcgagta accatttatg acattgcaaa aaaaatcaat gcttcaccga
     8461 gcactgtttc acgagttttg agcaacagtg attatcctgt cagccctgaa atgagaaaaa
     8521 aagtcttaaa agctgcaaaa gaaatgaatt actatcctaa catattggcc aggaatctga
     8581 agacaaaaaa cagcaaaaac gttggaatcg tattgccgtc aatatctaat cccttttatc
     8641 cttctgttgt tagaggaatc gaagatgtag cattaaaaaa tgattataca ttgtttatat
     8701 gcagctgtga agggaacaag gacagggaaa aaaagtattt tcaatatcta atggaaaatc
     8761 taagtatagg catcattact attttcaccg atctttatac agaaggcata caagagttca
     8821 ttgaacatgg cggagttgtg gtatctgttt gcgcagagcg agtggataat tcaggagttt
     8881 gctgcattta ctttgacaag gaaaaagaag gctacatggc tgcaaagcat ttgatagatt
     8941 taggccacga aaatattgcc attttaactc cgcccttaac caaccctttg agaattggga
     9001 aggttgaagg gtataagaga gccttaatcg aggcgggaat agccataaat caggattata
     9061 tctatattgc atctgaagaa tatgccccaa attacatgga caccatttat gaatgtaaca
     9121 ttgcttccca attgacaagg gatttgcttc agagaactcc ggaagtaacg ggaatcatct
     9181 gtattaacga cattatggca ctgggatgca tagccgaatt ggagagccat ggatacagag
     9241 tgcctgaaga ctattcggtg gtgggttttg acgatttgtt tttatcaaat ttggttaaac
     9301 caagattgac cactgtcaga tttgaaaaat ataatgtagg cagactggcc atggagatac
     9361 tacaaaaaat tgtagaaggg gaacagagga taaagcaata taattttagc aaatacacat
     9421 atttagaaaa aagggaaagc accggtcgac cgagagtaaa attatagaca gaatatcata
     9481 cacttttttc aattggcttt tttaatttac cctaatagaa atcgatttct ataattttct
     9541 attataccta gctattaaaa tctatcatta atttgattgc caaatcctta aggatttgca
     9601 ataaaaaatc atagggggag ttttaaaaat gaaaaaagtc ttaaaagtac tactggcagc
     9661 cacattggtt gttgcattac ttgcaggatg tggttctggt ggcacaggcg gctctggagg
     9721 aggttcacaa gaagcccagc ctgtaaagct aattgcagcc cacgtcaatc ctgaagatag
     9781 ttctttccat gcaggcatgg tagcttttaa ggaaaagctg gaagaattat ccggtggatc
     9841 aatgactgtt gaaatccatc acagcggtca gctgggtggc aatgaaaacg aacttgttga
     9901 aaaaatggca actggtactg ttgacgtaat cgtatcagct cccaactttg ttgctgcaat
     9961 cgtccctgaa gcagatttgt tctcaattcc ttacttatac gaagatgtag aacattggga
    10021 aagggttgtc actggagaag taggtcaaac cattctagat aaaattgaag ccggcacaga
    10081 ctttaaaccc ttaggttact ggatgtgtgg tgtaaggcaa tactttggta aaaaacccat
    10141 cacaactccg gatgacttaa aaggcgtaaa aatccgcgta cacaacagtg aaaccattca
    10201 gagcatatgg tcagccctag gagctcagcc ggcaatttta gcctacaatg aactgtattc
    10261 aggtttgcag aacaatgtca tcgatgcagc agaaaacgac ttcggcagca tactgcaaat
    10321 gaagttctat gaagtagggc catatatttc gctgaccaac catgacatag ccacaagata
    10381 cttcttgatg agcaaggcaa aatacaactc tctgacagat gaacaaaaag cctggatcga
    10441 cgaggcagct aaagcaagct gtgagcatca gtgggtattt gacagaaacc tgatagaaga
    10501 agctaaagtc aaattggaag aagccggtgt aaaattcaat gaaacggaaa tgcacctatt
    10561 cattgacaaa acccaggaag taagggaaaa ggcagctgaa aaactaggag tcagcgacct
    10621 attagaaaaa atcagtgaat taagagataa ctaaattaag aaagttaatg cggtgcggct
    10681 tatgccgcac ctgcacaatt aatttgtggt atttagccaa gcgtctctcc agtgaaagtg
    10741 actataaaga tttacgagat tagaccttgt attgagttca ttagcagaaa atccttcaaa
    10801 aggagggagt ttagatgaaa gaggcattaa gtaaattaga aagaatacta gtatacgtag
    10861 ggaacgcatg tttcgttctg ttctttgcaa tgactacaat tcaagttaca gcacgcgctg
    10921 taggttttaa ttctgcatac acggaagaga ttgccaacta cgcttttatt tggctggcat
    10981 ttgtgggttc atccatctgc ataagatcag atgaacattt cagatttacc gctttcaccg
    11041 agaggttgtc ggagaaaggc aaattaataa a
//
LOCUS       DAONUO010000019        11054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4550_length_11054_cov_4.002637, whole genome shotgun sequence.
ACCESSION   DAONUO010000019 DAONUO010000000
VERSION     DAONUO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4550_length_11054_cov_4.002637"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..234)
                     /locus_tag="PK768_01320"
     CDS             complement(70..234)
                     /locus_tag="PK768_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59644.1"
                     /translation="MKDIKPVLDQVLDELDKTFLDRYLKEEKEEIYKLPTSTDIKIDV
                     DWREEANAAD"
     gene            complement(231..1103)
                     /locus_tag="PK768_01325"
     CDS             complement(231..1103)
                     /locus_tag="PK768_01325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQA59645.1"
                     /translation="MASKQVEFDKKHKFLILETIYESEATQTCFYKAKDMEYDRTVGI
                     KIICFDDFKQRDLLLQEARSMIKLEEFTDKIPSLYEYHIDNKNKKLILVMQYIDGLTL
                     REIIKQTGDKIKDPYVIRQNIELIYQISLVLGAIHTSAPVFQHKDLKPENIIVKNKGQ
                     LNQAVYLIDLGITGRPLRRKTGTPLYQSPEQRGYLGFISSDNVDVFSLGLIFFEILTG
                     KQLRIGVDLLRKSNEKHWVNIPAVTDYNENVPPIYNDIISKCLQLMPDKRYRNGMEVA
                     KAINYALKRKRYGR"
     gene            complement(1096..2439)
                     /locus_tag="PK768_01330"
     CDS             complement(1096..2439)
                     /locus_tag="PK768_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59646.1"
                     /translation="MYFDALKDVQTHAKNELYETCLAAEENARIRPNESVINARKALE
                     VFTASVLADHGITIPSDKSERQSKGLSDLYGQIRKCRWENFIDKNTYHLLDEIRRDAN
                     KVVHVTYNDAGVPEKYEIKNIDADITLASKLVKNLYEGIVNHFLKGKRRLTLDVERLP
                     FGKYQVVELVKKTDMEASAGKFNYICKRVDLDENNRERCSWAYIRAFKKEPEIYNRRD
                     STVLYEVWEGIHELPRNIVMGSVIDTSRECGLIYIKYNIDQRTFTLANRERLGKVTAK
                     AALEIIEKLIIALLSIQNVKAGTKIYHRGIRPEYVFVTPQNNSFSVSLGCFETSKIVK
                     DDEREAYTTVYHTMKEKQTSNPFAPYELRNNLIQDTDGVDWEKVDTYSLGVLLLWILG
                     DDIRSDKQINIDILIDHYSDAFVDFMEQVIENSLNIKPSLREFLEALRKELVLVG"
     gene            complement(2630..3154)
                     /locus_tag="PK768_01335"
     CDS             complement(2630..3154)
                     /locus_tag="PK768_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HQA59647.1"
                     /translation="MGKIKTPEPVTPIASVFTSDEKLFESAKEILSKELGPCIYESTV
                     LPFNHTDYYTAEMGSGLKRRIFAFEKLVDPSKLPALKLWSNSIEEDWAVGGKRQVNID
                     VGYVSLGKLVLASTKNHSHRIYLGEGIYGEVTLRFVGGEFQPWPWTYPDYASEEYRKI
                     FGGIRQLHKEKLRS"
     gene            3316..4104
                     /locus_tag="PK768_01340"
     CDS             3316..4104
                     /locus_tag="PK768_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQA59648.1"
                     /translation="MGIVGGGRAGTLFLKTLLEIPEVDVIGICDKNPEAAAIVFAQEH
                     GIPTYLDLRLFLENHMDVVLELTGVPEVSNTIGQLKKPGTHLMDSHAAKLASLLTEHQ
                     QELSEKLADYIDHIKELLVNVTSNISAINDTVLSIDSTSKSILESVSSAIESINKTDQ
                     IIEIINGITSQIRILGINASIEAAHAGEMGKGFSVVASEIGNLTTSSQNATSEIKAII
                     DKMKADINKLSGIANTLEIICNKQREVASSLSDDNAKMSSIISK"
     gene            complement(4162..7200)
                     /locus_tag="PK768_01345"
     CDS             complement(4162..7200)
                     /locus_tag="PK768_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQA59649.1"
                     /translation="MKIKFKEQQFQIDAVQSVVDCFRGQPNQVSHFQLDRGMAKGEIA
                     GQQMLLLESGFKNNPIMLSEEEVLENIRKVQLRNGLKPSASLAGRYNLTIEMETGTGK
                     TYTYIRTMYELYRAYGWSKFIIVVPSIAIREGVYKSFQMTEDHFMDLYGTKIRYFIYN
                     SKQLHKIDQFASDSGINAMIINSQAFNARGKDARRIYDELDEFGSRRPIDLLSQTNPI
                     LIIDEPQSVEGKKTKEALKLFNPLFTLRYSATHKEEYNKIYRLDALDAYNMKLVKKIK
                     VKGISVKGFTGTSAYIYFEGIDVSSKRKPIARIEFEVKQKTGIKRVSRNVDEGFDLYT
                     HSNYMEQYKGYRVSEINGYKNTISFINGVALSAGDVQGDVSESTLRRIQIRETIRSHF
                     ERERELYSKGIKVLSLFFIDEVAKYRQYDEYGNQVNGEYGKIFEQEYLNILNEYITDS
                     DDPYLEYLKSIDVKDTHKGYFSIDKQGRMIDPQAKGREKISDDESAYDLIMRDKERLL
                     SFDEPTRFIFSHSALREGWDNPNVFQICTLKHSDSVIRKRQEVGRGLRLCVDQNGNRI
                     DGETPGVNVHDVNILTVMASESYETFAKGLQSEIAETLSDRPQKANVEFFLNNVVKNA
                     RGEELFIDENLAKTLHRSFIRNLYVDDNDNLTDKYYEDLEAGKLKLPYELRDFEEGIV
                     QLVCKIYSEGATPLVEDSRAENIKKLEVNENFKKKEFEELWNKINQKTAYYVDFDTEE
                     LIENCIEALDSGLKVSEITFQVKTGEMDSISSKEELERGEAFKVKETATDKEYKPVTT
                     TVRYDLVGKLVDETKLTRKAIVKILKGIQPKTFDQFKKNPEEFILKVSRIINEQKATM
                     IIQHITYNPIDEVYDSSIFTKNTLRGTFGKDAIEVKKHIYDYLVTDSEKEKEFAKALD
                     ASSEVSVYAKLPRGFLIPTPVGNYNPDWAIVFNEGKVKHIYFVAETKGKLESLHFDTR
                     GIEEAKIHCAKKHFEKISSSTVKYEVVDSYEKLMDKVMGQ"
     gene            complement(7211..9124)
                     /locus_tag="PK768_01350"
     CDS             complement(7211..9124)
                     /locus_tag="PK768_01350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQA59650.1"
                     /translation="MEKLKMKSKDITRENIEIIGKLFPNVITETEDTNGNVSRAIDFE
                     LLKQELCDEIVEGSKERYQLTWPGKKEALILANTPTNKTLRPVKEDSLNWESTQNIFI
                     EGDNLEALKLLQEAYLNKIKFIYIDPPYNTGNDFIYKDKFTIDKTKYAKKSGQMDQEG
                     NRLFLITEYHGRYHSDWISMIYPRLKLARNLLSEDGVILISIDDNEIHNLRRVCDEIF
                     GERNFVANLIWRKSKGSGNDSKYLMVETEYILLYAKNIENLKFKNQLVDIDDGKYKYK
                     DEYYEERGGYNLEKLDRGSKGYVESLDFGIEAPDGTLVFPNNRREKYNDGWRWMWSKS
                     KVEWGIKNGFIVVKKGKDNQWNVYCKNYAKVDNEGNPIVRTRLYRNHIGFEETILNIQ
                     ANHEMKKLFAGALFSYPKPTNLLKYLIDMFYLDNEIILDFFAGSATTADAVMQLNAED
                     GGNRRYIMVQLPEACDEDSEAYKAGYKNIAEISKERIRRAGKKIKEETGADIDYGFRV
                     FKVDSSNMKDIYYTPDKLNQSILDELESNIKEDRTGLDLLTQVMLEAGLELSLPIETK
                     DICGREVHFVAGNSLAACFDENLDEDFIRQIAKHEPLKAVFRDSSFSSCPDRINLEEI
                     FKGISPDTQIKVL"
     gene            complement(9633..9854)
                     /gene="csrA"
                     /locus_tag="PK768_01355"
     CDS             complement(9633..9854)
                     /gene="csrA"
                     /locus_tag="PK768_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HQA59651.1"
                     /translation="MLVLTRKIGEGIIVGNDVIIKVFEIEGERVKIGIEAPKTTKVLR
                     LELYEDISMENKKAVHVSKNAVDKLFELF"
     gene            complement(9848..10279)
                     /locus_tag="PK768_01360"
     CDS             complement(9848..10279)
                     /locus_tag="PK768_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HQA59652.1"
                     /translation="MVTTESKKIITFVNGILGLEQYKSYVLLDHPGTDIVKWLEAVDQ
                     PEIALPVVNPFLFFPDYAPKISRENLLQINIKSPEEAIVLCVLTIGANTKDITVNLKA
                     PIIVNSDSRLADQFIADNPEYNVREPLKLKKACSDGRCESC"
     gene            complement(10290..10871)
                     /locus_tag="PK768_01365"
     CDS             complement(10290..10871)
                     /locus_tag="PK768_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6470 family protein"
                     /protein_id="HQA59653.1"
                     /translation="MLIYNLDIQFAFGRIGVDTYKGSVDPKMNIHEGVDINTRLPEIA
                     LSRSLPVIEDIDASHCWADMGQPKLFSAIPMWYNEAKQYALEYIGSKAAGGDALGAIE
                     KGISIGDIVESESFSDAPEVNVKAVPKTPPKITFKMGKVNTNFIQGSVKVNSSDKTVT
                     IDYERANVNIFLEQNPYINVKAVPTGKNIDKIA"
BASE COUNT         2801 a         2427 c         2102 g         3724 t
ORIGIN      
        1 acccgaacat tattgccgat aaaaaatatc aggggtgaat ctacagcata ccattcaccg
       61 gaatataggt taatctgctg cattagcttc ttctctccaa tccacatcta tttttatatc
      121 agtggaagtg ggtaatttat aaatttcctc tttttcttct ttaaggtatc tatcaagaaa
      181 agtcttgtcg agctcatcca atacttggtc cagtaccggt tttatatctt tcaccttcca
      241 tacctctttc tcttaagggc gtaatttatg gcctttgcca cttccatacc gtttctatat
      301 cttttgtcag gcattagctg caggcatttg cttataatat cgttatatat aggaggaaca
      361 ttctcattat aatccgtaac ggcaggaata tttacccaat gcttttcatt gctttttctc
      421 aataagtcta ctccgatcct cagttgtttg cctgtgagta tctcaaaaaa gattaagcct
      481 aaagaaaata cgtctacatt gtcagacgat ataaatccta aatagcctct ctgctccggg
      541 gattggtaga ggggtgtgcc ggtttttcgt cgcaatgggc gaccggtaat gcctaaatca
      601 attaaatata cagcttggtt cagctgaccc ttgttcttga caataatgtt ttccggtttt
      661 aaatccttat gctgaaacac gggagctgac gtatgaattg cacccaatac caggcttatt
      721 tggtaaatca attcaatatt ttgtctgatc acataaggat cttttatctt atcccctgtt
      781 tgtttaataa tttctctcag tgtaaggcca tctatgtact gcatcaccag gataagtttt
      841 ttatttttgt tgtcaatatg gtactcgtag aggctgggaa tcttatcggt gaattcttcc
      901 agttttatca tactcctggc ttcctgcagc agtagatccc tctgtttaaa atcatcaaag
      961 catataatct tgattcccac agttctgtcg tattccatat ctttcgcctt atagaagcag
     1021 gtttgagtgg cctctgattc ataaatcgtt tccaggatga ggaatttgtg ttttttatca
     1081 aactcaactt gtttgctagc caactagaac cagctctttt ctcaaagcct ccagaaattc
     1141 cctaagtgaa ggttttatat ttaaggaatt ctcaattact tgctccataa aatctacaaa
     1201 ggcgtcactg taatggtcta tcaagatgtc tatgttaatt tgcttatctg aacggatgtc
     1261 atcacctaaa atccataaaa gtaatacacc cagggaatat gtatcgactt tttcccaatc
     1321 cacaccatcc gtatcttgaa ttaagttatt ccttagttcg tatggggcaa atggatttga
     1381 ggtctgtttt tctttcatgg tgtgatatac cgtggtgtaa gcctcccttt catcatcctt
     1441 tacgatcttg gatgtctcaa aacagcctaa gcttacactg aaagaattgt tttgcggcgt
     1501 cacaaagaca tactcaggtc taatccccct atggtaaatc ttggtaccag cctttacgtt
     1561 ttgaattgaa agcaatgcga taatgagctt ttcaatgatt tccagcgccg ccttggccgt
     1621 cactttgccc agtctttctc tgttggctaa agtaaaagtt ctttggtcga tattgtattt
     1681 aatataaatc aggccacatt ccctagaagt atcaattaca ctgcccataa ctatatttcg
     1741 cggcagttca tgaataccct cccaaacctc atacaaaaca gtactgtcac gtctgttgta
     1801 tatttccggc tcctttttaa aagctctaat atatgcccag ctgcagcgct cacgattgtt
     1861 ctcatccagg tcaacgcgct tgcatatata attgaatttt cctgcagaag cttccatatc
     1921 agtttttttg accaattcca caacctggta tttgccaaac ggcagccgct ccacatctag
     1981 agtaagtctc cttttcccct ttaaaaaatg attcacaatc ccttcataaa gatttttaac
     2041 cagttttgat gccagtgtaa tatccgcatc aatatttttt atctcgtatt tttccggaac
     2101 accagcatcg ttgtacgtaa catggacgac tttatttgcg tctcttctta tttcatcaag
     2161 caagtgatat gtatttttgt caataaagtt ttcccagcgg cattttctga tttgtccgta
     2221 taaatcgctc aaacccttgc tttgcctttc agatttatct gaaggtatgg taatgccgtg
     2281 gtcagccagt actgacgccg taaagacttc caaagccttt cgcgcattga ttacactttc
     2341 attgggccga attcttgcat tttcttcagc agccaagcac gtttcataaa gctcattttt
     2401 agcatgggtt tgcacatctt ttaacgcatc aaaatacata agaaccaccc cattctaaaa
     2461 acacaatgcc aaatgaatat tactatgtat acatttctac agtcttatag aaaatccttc
     2521 ctttaagcaa gttggagatg ccgaaaattc tttgtattcg tattattttg ttccatattg
     2581 tcgataaaag cagcattgca aaacaaaaaa gccgcaatga atgcggttac taggagcgaa
     2641 gtttttcctt gtgcagctgg cgaatcccgc caaagatttt gcgatactct tcacttgcat
     2701 aatcgggata ggtccagggc cagggctgaa attctccacc gacaaaacgc agggtaacct
     2761 ctccgtagat cccttcacct aaatagatgc gatggctgtg gttttttgtg ctggcaagga
     2821 ccaacttacc aaggcttaca taacctacat cgatattgac ctgccgtttg ccgcctactg
     2881 cccagtcctc ttcgatagag tttgaccaaa gctttaaggc aggaagtttg ctaggatcaa
     2941 caagcttttc aaaggcgaat atccgacgtt ttaggccact acccatttca gcggtgtagt
     3001 agtcggtgtg attaaagggc agcaccgtgc tttcgtaaat gcaaggtcct agctcttttg
     3061 acaagatttc ttttgcagat tcaaataatt tttcatctga agtaaaaacg ctggcaattg
     3121 gagttaccgg ttctggcgtt tttatttttc ccatactcat ccccttttct caattcatat
     3181 agcttgatta aaaatccgaa aggttctttc cattgaagga ttttatcaaa ttttgtcgaa
     3241 taatacctga aagtttattt ccatattttt gatggattac ttcaaatgtt ttttcaagga
     3301 ggcagcacat tgaaagtggg aatcgttgga ggcggcaggg ccggaacact gtttctcaaa
     3361 acacttttag aaatacccga ggttgatgtc ataggcattt gcgataaaaa cccagaagcg
     3421 gcggctattg tttttgcgca ggagcatggc atccccacat atttggacct aagactattt
     3481 ttggaaaacc atatggatgt agtgttagaa cttacggggg ttccggaagt aagcaatact
     3541 attgggcagc ttaaaaaacc aggaactcac ctcatggatt cccatgcggc aaagctcgca
     3601 tctcttttga cagaacatca acaggagctt tcggaaaaac tcgctgacta tatagaccac
     3661 atcaaggaac tgcttgtgaa tgtaacatca aatatttcag caatcaatga cactgtttta
     3721 tcgatagaca gcacgtctaa aagcatcctt gagtcggttt caagtgccat agaaagtata
     3781 aacaaaacag accagatcat tgaaatcatc aacgggatta catcacaaat tagaattctt
     3841 ggtataaacg cctctatcga ggcagcccat gctggagaga tgggtaaggg cttttcggtg
     3901 gtggcatcgg agataggcaa tcttaccaca tccagtcaaa acgcaacatc tgaaataaaa
     3961 gccatcattg acaaaatgaa ggcagacatc aacaagctct cggggattgc gaacactctt
     4021 gaaattatct gcaacaaaca aagggaagta gccagctctc tttctgatga caatgcaaaa
     4081 atgagcagta tcataagtaa ataataattt tgggtggtgg atgttctcca gtgtcgtcta
     4141 ttgtgctgtt ggttgccgac tttattgtcc cataactttg tccattaact tttcgtaact
     4201 atccaccact tcgtacttta cagtgctgct gcttatcttt tcgaagtgct tttttgcaca
     4261 gtggattttg gcttcttcta ttccccttgt gtcaaagtgc agggattcaa gcttgccctt
     4321 ggtttcggcg acaaagtata tgtgttttac cttgccctcg ttgaagacta tagcccagtc
     4381 gggattataa ttgccaacgg gagttgggat taagaagccc cttggcagct tggcatagac
     4441 gctaacttca ctgctggcat caagggcttt ggcaaattct ttttcctttt cagaatcggt
     4501 aaccaggtag tcgtatatgt gctttttcac ttctatggca tccttgccaa aagtccctcg
     4561 caaggtattt ttcgtaaaaa tactactatc gtatacttca tctatgggat tgtatgtaat
     4621 gtgctggatt atcatcgtgg ccttttgttc atttatgatc ctggagacct tcagtataaa
     4681 ttcttccgga ttcttcttga actggtcaaa ggtcttaggc tgtatacctt ttaggatctt
     4741 cacaatggcc tttcttgtaa gctttgtctc atctaccagt ttccccacta aatcgtatct
     4801 tacagtagtt gttaccggtt tgtattcctt gtccgtggcc gtttctttga ctttaaaagc
     4861 ctctcccctt tccagctctt ctttggagga aattgagtcc atttctccag ttttcacttg
     4921 aaatgttatt tcagacacct ttaagccgct gtccagggct tcgatgcagt tttcaatcaa
     4981 ttcttccgtg tcaaagtcca catagtatgc cgtcttttgg tttatcttgt tccagagttc
     5041 ctcgaattcc tttttcttga agttttcatt gacctccagc tttttgatat tctccgccct
     5101 gctgtcttcc acaagaggtg tggcaccttc actgtatatt ttgcatacaa gctggactat
     5161 gccctcttca aaatccctca gttcatatgg aagtttgagc tttcctgctt ccaagtcctc
     5221 ataatattta tcagttaaat tgtcattgtc atcgacgtaa agattcctta taaaggagcg
     5281 gtgaagggtt tttgcaaggt tttcatcaat aaagagctct tctccccgag cgtttttaac
     5341 cacattgttt aagaaaaact ccacattggc tttctgtggc ctgtcggata aggtttcggc
     5401 aatctctgac tgtaagccct tggcaaaggt ttcataagac tcgctggcca ttacagtgag
     5461 gatatttacg tcgtggacat ttacccctgg agtttcaccg tctatcctat tgccattttg
     5521 gtcaacacag agtctaaggc ccctgcccac ttcctgcctt tttctgatta ctgaatcact
     5581 gtgctttaaa gtgcaaattt gaaatacgtt ggggttgtcc cagccttccc ttaaggccga
     5641 gtgggaaaat ataaatcttg tgggttcgtc aaagcttagc agtcgttctt tgtccctcat
     5701 tatcaaatca tatgcagact catcatctga tattttttct ctacctttag cctgtgggtc
     5761 tatcattctg ccctgtttgt ctatggaaaa atatcctttg tgggtatcct ttacatcgat
     5821 agattttaaa tactccaaat aggggtcatc agagtccgtg atgtattcat ttaagatatt
     5881 taagtactct tgttcaaaaa ttttaccgta ttcgccattg acctgattgc cgtattcatc
     5941 atactgtctg tacttggcca cttcatctat gaaaaagagg ctgagtacct ttataccttt
     6001 gctgtatagt tctctttccc tttcaaagtg ggaccttatg gtttcccgaa tctgtatcct
     6061 tcttaaagtt gactcgctga catctccctg gacatctccg gcagaaaggg caactccgtt
     6121 gataaagctt atggtgtttt tgtagccatt tatttcagat acccggtatc ccttgtactg
     6181 ctccatgtaa ttggaatgag tgtacaaatc aaaaccttca tctacatttc tgctaactcg
     6241 ctttatgccc gtcttctgct tgacctcaaa ctctatcctg gctatcggtt ttctcttgct
     6301 gctgacatct atgccttcaa agtagatata tgcactggtg cccgtaaagc cctttacaga
     6361 aatacccttc actttgattt ttttcaccag tttcatgttg tacgcatcaa gggcatccaa
     6421 gcggtatatt ttgttatatt cctctttgtg ggtagcagaa taccttaaag taaacagggg
     6481 gttaaagagt ttcagtgctt ccttggtttt cttgccttcc accgattgag gctcgtctat
     6541 tatgaggatg gggttggttt gagacaagag atctattggc cttcttgagc cgaattcatc
     6601 cagctcatca tagattcgcc gggcatcttt tccccgggca ttaaaagctt gagagtttat
     6661 tatcatggca tttatccctg aatcagaggc gaattggtct atcttgtgaa gctgcttgga
     6721 gttgtagata aagtaacgga tttttgtacc gtataaatcc ataaaatggt cttcagtcat
     6781 ttggaaggat ttgtacaccc cttcccggat agcaatggac ggcaccacta tgatgaactt
     6841 gctccagccg taggctctgt atagttcata cattgtcctt atatatgtat aggttttgcc
     6901 ggtgcccgtc tccatttcga tggtcagatt atatctgccg gctaagctag cagagggctt
     6961 taaaccgttt cgcaattgga cttttcgtat gttttccaac acttcttctt ctgacaacat
     7021 tatgggattg tttttaaagc cgctttcaag caagagcatt tgctggccgg caatttctcc
     7081 ctttgccata cccctatcca gttgaaagtg ggagacctga tttggctgcc ctctaaaaca
     7141 atccactaca gattggaccg catctatctg aaactgctgc tctttaaatt ttatcttcat
     7201 aaaatcacct ttataaaacc ttaatctgtg tatcaggaga tatgccttta aagatttctt
     7261 caaggttaat tctgtctggg catgaactaa aggagctgtc ccgaaatact gcctttaaag
     7321 gttcatgttt tgctatctgt ctaataaaat cttcgtccag gttttcatca aagcaggccg
     7381 ccaaggaatt gcctgccaca aagtgaactt ccctgccgca aatatctttg gtctctatgg
     7441 gaagggaaag ttccaatcct gcttcaagca ttacttgtgt cagaagatca aggccggtcc
     7501 ggtcttcttt tatattggat tctaattcat ctaagatgct ttgattaagc ttatccggtg
     7561 tataataaat gtctttcatg tttgatgaat ccactttgaa tactctaaag ccgtagtcga
     7621 tatcggcacc ggtttcttct tttatctttt tcccggcacg gcggatccgc tccttggata
     7681 tttcggctat gtttttgtaa cctgctttgt aggcttccga gtcttcgtca caagcttcag
     7741 gaagctggac cattatgtat cttctattac cgccatcttc tgcattgagc tgcatcaccg
     7801 catctgcagt agtggctgaa cctgcaaaga agtccaggat gatttcattg tccaggtaaa
     7861 acatgtcaat taaatatttt aacaggtttg tcggtttcgg ataggaaaaa agggctcctg
     7921 caaaaagctt tttcatctcg tggttggcct gtatattaag tatggtttct tcaaaaccga
     7981 tgtgattcct gtagagtctg gtccttacaa tgggattgcc ttcgttgtcc actttcgcgt
     8041 agtttttgca gtacacattc cactgattat ctttgccttt tttaactact ataaagccat
     8101 tttttatgcc ccactctacc ttcgatttgc tccacatcca gcgccagccg tcattgtact
     8161 tttctctcct gttgtttgga aaaaccaagg tcccatcagg agcttctatg ccaaaatcca
     8221 ggctctccac ataccccttg cttcccctgt ccagcttttc taagttgtat ccgcctcttt
     8281 cttcgtagta ttcatccttg tatttgtact ttccgtcgtc gatatctact agttgattct
     8341 tgaactttag attttctatg tttttggcat aaaggagtat gtattccgtt tccaccatga
     8401 ggtactttga atcattgccg gagcccttgg attttcgcca aattagattt gccacaaaat
     8461 ttctctctcc aaagatttcg tcgcagaccc ttctcaagtt gtggatttca ttgtcatcga
     8521 tgctgatgag gattacccca tcttcagaca gcaggttcct ggccagtttt aatctcgggt
     8581 agatcatgct gatccaatcg ctgtggtacc ttccgtggta ttcggtgatt agaaagagcc
     8641 tgttgccttc ttgatccatt tgccccgatt tttttgcata tttagtttta tctatggtga
     8701 atttgtcctt gtatataaaa tcattgcccg tgttgtaagg tgggtctatg tagataaatt
     8761 ttatcttgtt taaatatgct tcctgaagga gtttcaaagc ttcaagattg tctccttcga
     8821 taaagatgtt ttgtgtgctt tcccagttta ggctgtcttc tttcacaggc cttagggttt
     8881 tgttagtggg agtattggcc aaaatcaagg cttctttctt gcctggccat gtaagctggt
     8941 atctttcctt gctgccttcg actatctcat cgcagagttc ctgctttaac agttcaaaat
     9001 ctatagcacg gcttacgttg ccatttgtat cttcagtttc cgttatgaca ttcggaaaga
     9061 gtttgccgat tatttctatg ttttcccggg taatatcctt tgatttcatt ttcagctttt
     9121 ccatttgaag acctccttca tgaacttttg ataaaaatta ctattctatt tgggtttgtg
     9181 ctggcattag ttctgctttc tgttatgggg taataacaac ataatgtcaa taatccaatg
     9241 ataatgcggg accttttacg tcagcaattt taatatgctc aactttggta aaaggatatt
     9301 gaacgccgtt ttggtcaagg gaaaattatc gattagtgtc aaatcgtagg gcgaagataa
     9361 cggcaaacgc tacctttaag gtagaccgtc gtcattgtta agtttggtcc aaacgcagca
     9421 ggggcaaagt ttacaatgac aggtctaact ttagggagcg gtgccgttat ctcgcccgtg
     9481 attttgacgt catagttccc ataatttctt gctataggaa atgttttgct taaaacttat
     9541 tatatcatgg ccatgttgat aagtacacca caaaaccgca gaatctacaa aagacctgcg
     9601 gtttttattt gcgaaaaaat cttatgatac atttaaaaca gttcaaaaag cttgtcgacg
     9661 gcatttttag agacatggac agcctttttg ttttccatac tgatatcctc gtatagttcc
     9721 agtctcaata ctttggtggt ctttggagct tcgatgccga tcttaacccg ctcgccctcg
     9781 atttcaaaaa cctttataat cacatcattg cctacaatta tgccttctcc aatctttctt
     9841 gtaagtacta gcaagattca caccgcccat cagaacaagc ttttttgagt ttcagcggct
     9901 cccggacatt gtattccgga ttgtcagcta tgaattggtc tgccaaacgt gaatctgagt
     9961 ttacaatgat tggcgctttg aggtttacgg taatgtcttt tgtatttgca ccgattgtca
    10021 gcacacaaag gactatggct tcttccggag acttgatatt tatttggaga aggttttccc
    10081 gggaaatttt tggagcatag tccggaaaaa aaaggaaggg attgaccacg ggcagggcta
    10141 tttccggttg atctacagcc tcaagccact taacaatatc ggttcccgga tggtccagca
    10201 atacataact cttgtactgc tcaagaccta atataccgtt aacaaatgtg ataatctttt
    10261 ttgattccgt tgtcaccaca acacatctcc tatgcaatct tatctatatt tttgcccgtt
    10321 ggcaccgctt tcacgtttat atacgggttt tgttcaagaa atatattgac atttgccctt
    10381 tcataatcta tcgtaacggt tttatcagat gaattgacct ttacgctgcc ttgtatgaaa
    10441 ttcgtattta ctttgcccat tttaaaggtt attttaggcg gtgttttggg gactgctttc
    10501 acatttactt caggcgcgtc ggaaaaggat tcgctttcca ctatgtcgcc aatagaaatg
    10561 cccttttcta tagctcctaa tgcatcaccg ccggcagctt tgctgcctat gtattccagg
    10621 gcgtactgtt ttgcttcatt ataccacatg ggaattgctg agaaaagttt gggctggccc
    10681 atatcggccc agcagtggga cgcatctata tcttcaataa caggaaggct tcttgaaagg
    10741 gcaatttcag gcaatcgggt gttgatgtca acaccttcat gtatattcat ttttgggtcc
    10801 acagaaccct tgtatgtgtc aactcctatt cggccaaaag caaattgaat atccagattg
    10861 tatatcagca tatttggacc tcctaaggac ttctcggatg ctttatctca ggaagtccac
    10921 taaggtaggt tgaattattc ttgcacccac ggcaagggat gccctatata tgctctcttc
    10981 catcttaagt tccgttatca ttttagccag atcaattccc tcgacgctgt tgagtagatc
    11041 taaattatca attt
//
LOCUS       DAONUO010000020        10948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4611_length_10948_cov_3.592307, whole genome shotgun sequence.
ACCESSION   DAONUO010000020 DAONUO010000000
VERSION     DAONUO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4611_length_10948_cov_3.592307"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1679
                     /gene="uxaC"
                     /locus_tag="PK768_01370"
     CDS             276..1679
                     /gene="uxaC"
                     /locus_tag="PK768_01370"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HQA59654.1"
                     /translation="MKKFMDEDFLLSNDTAKTLYHENAKQMPIVDYHCHIDPKDIYED
                     VVYANLTQVWLGGDHYKWRAMRTNGIEERYITGEDTTAYEKFEKWAETVPKLIGNPLY
                     HWTHMELKRYFDIDDILSPKTCKKIWDAANERLKDLSVRKIIEKSNVKLICTTDDPID
                     DLRWHKYISEDETCKIKVLPAFRPDKVLNIDKPGFVEYIKKLGDVCGINIKTVDDVKA
                     ALASRIEFFVKMGCKASDHALDYIVYRKTDENLDAVLQAALDGKAIDQEKADEYKAEL
                     LMFCASQYKKHDIVMQLHYGALRNTNPAAFAKLGPDSGFDAICGYDKSGVALGALLGA
                     LEAKDSLPKTIIYSLNPTDNALITAIIGCFQSPEVPMKIQHGSAWWFNDTKTGMMEQM
                     TNLANFSILPNFIGMLTDSRSFLSYTRHEYFRRILCDLIGQWVENGEYPADMEILGKM
                     VEDICYNNTVRYFGFEV"
     gene            complement(1830..3191)
                     /locus_tag="PK768_01375"
     CDS             complement(1830..3191)
                     /locus_tag="PK768_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQA59655.1"
                     /translation="MKLNPNVFRQYDIRGIVGEDLDESFATRLGQAFGTYARQEGENK
                     AIVGCDNRTSSPLLKKAVINGLLSTGCDVVDIGTVVTPIFYFAREHYGINPGIMVTAS
                     HNPAQFNGFKVAFGPGTIYGDEIQYLRRMMEKGEFLSGKGSVSSKDPVEDYMDMISSK
                     IRLSKKLRVGIDCGNGTASLFAEKFFSRLGVEVYPLYCTSDPSFPNHFPDPVNAENLA
                     DLRELVLREQLDLGIGFDGDGDRIGVVDDRGNVIFGDMLMILYWREIMPKYPGSTAIV
                     EVKCSEALVDEIKKLGGKPIFYKTGHSLIKAKMREVGAIFTGEMSGHMFFADEYYGFD
                     DAFYAAARLLRILSNTDSKLSELLSDVPKYFSTPELRIPCPDEEKFDKVLAVKRYFEG
                     KYPIIDVDGARVLFSDGWGLVRSSNTGPELIVRCESKTEEGLEKIKREMQEALLPLKL
                     FNG"
     gene            3407..4513
                     /gene="hisZ"
                     /locus_tag="PK768_01380"
     CDS             3407..4513
                     /gene="hisZ"
                     /locus_tag="PK768_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQA59656.1"
                     /translation="MTGYFRRPAGVKDYLPPKVRKKRRIEKVIVDIFEKWGYEEVIAP
                     SFEYLETFAKTGKWDFTEKIFKFIDRHGEAVALRPDITTSIARMVATHYSNHDAPLRF
                     YYLANVFRFQEPRKGRDQEFYQIGAELIGIPGLEADVEIILLASHMLEQIGIKDFLIN
                     IGHTKFLEGLLDELENSSSLKDRIAQAIKEKNFVQLKDLIEESAIRPEIKKTVLALPR
                     FYGGAEIFEKLDQFTLNEKCIAALSELKKVWNMLKQFNNIQLSFDPGLARGIDYYTGI
                     VFEIYSPKSGFPLGGGGRYDSLLDKFNGSRPATGFALTEDVILTVLEKDMEDTYEPYY
                     LYYNPAEYIEAFHKAEDLRRQGRIVKMLPADKYD"
     gene            4544..5158
                     /gene="hisG"
                     /locus_tag="PK768_01385"
     CDS             4544..5158
                     /gene="hisG"
                     /locus_tag="PK768_01385"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQA59657.1"
                     /translation="MLKIALSKGRILSETLKLFNKIQMDFSQVNEDTRKLFFDFEEYG
                     TRVILAKPSDVPTYVEYGVADIGVAGKDVLLEDKKDVFELVDLKLAQCKMVVAIPQDR
                     SIDMPIYRVATKYPQIAERHFVKRGIPVEIIKLHGSVELGPILGISDAIVDIVSTGST
                     LKKNNLKILEEICPISSRLIANQVSFRVKSYEIMALIEKLESVL"
     gene            5238..6311
                     /gene="hisC"
                     /locus_tag="PK768_01390"
     CDS             5238..6311
                     /gene="hisC"
                     /locus_tag="PK768_01390"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQA59658.1"
                     /translation="MRFARDDVLKMIPYMPSQEACKIKLDANESPYDVPLKVKERIWK
                     RLQEEHFNYYYDPSCDELRESLSKYTGAKPQQIFVGSGADEIILDLILAFAGPGRDVI
                     IPSPTFSSYEIFATVAGSNVIKVPIHRIKQGNGWIWDLDINQIKEHFTQDKDQLLFIC
                     YPNNPTGDYFDEEKVLDLINSFNGIVAVDEAYFEFGGKTFADRLHQFPHVVVIRTFSK
                     IFSLAGLRVGYAIGHEDLINQLYKVKLPYNVSLFSQIAAVEILKETDWLEDMKRQFAK
                     ARDELKEELDNIKGLRVYPSAANFFLCELEKSRDFVYEELLKRGILTRRLSDDILKNT
                     LRFCIGTPEQNRFLISSLREILD"
     gene            6330..6917
                     /gene="hisB"
                     /locus_tag="PK768_01395"
     CDS             6330..6917
                     /gene="hisB"
                     /locus_tag="PK768_01395"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HQA59659.1"
                     /translation="MARTAEVYRKTGETEIKVKMNLDGQGENKITTQVFFLNHMLDLF
                     SRHGLFDLEVDGKGDIEVDFHHLVEDVGIVLGQAFLKALGNKEGIKRYGTFYVPMDEA
                     LAFCSVDISGRPYIYFDAEFSRDKVGEFDVELVKEFFVAFANNARITIHLEVVRGENT
                     HHMIEALFKAFGRALREAVAFDPREKGVPSTKGVL"
     gene            6937..7665
                     /gene="hisA"
                     /locus_tag="PK768_01400"
     CDS             6937..7665
                     /gene="hisA"
                     /locus_tag="PK768_01400"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQA59660.1"
                     /translation="MNIYPAVDIKGGKCVRLVQGDFSQETVYLENPVDAAKMWQEKGA
                     KWVHVVDLDGAKSGNPGNFDVIRNIREALSAKIQVGGGIRNIGTVESYLNMGIDRIIF
                     GSSAITDLTLVKVTIDRFGAEKVAVGIDVRQGHVAIEGWTKSSGITVEVVLRQLKDIG
                     VNTIIYTDISRDGMMKGPNFAGIEKVLSFGDFSVIASGGISSLEDLEQLSMYENRGLE
                     GAIIGKALYNGSLDLKTVLERFDT"
     gene            7686..8432
                     /gene="hisF"
                     /locus_tag="PK768_01405"
     CDS             7686..8432
                     /gene="hisF"
                     /locus_tag="PK768_01405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQA59661.1"
                     /translation="MNKKRLIVCLDVQDGRVVKGIHFKNIRDIGDPVELAAYYDRQGI
                     DEIVLLDISATFEGRKTTLDVIKRVSENIRVPFTVGGGISTLKDIEDLLKAGATKVSI
                     GSAAVKNPQLIKEAAQEFGNGHLVLACDAKKNNGSWEVYTHGGRTATGLDAVLWCKKA
                     ESLGIGEILLTSMDADGTKDGYDIRLTRAVADAVKVPVIASGGAGKLEHFKDVFVKGR
                     ASGALAASVFHKGIFTVREVKEYLISEGVE"
     gene            8436..9071
                     /gene="hisIE"
                     /locus_tag="PK768_01410"
     CDS             8436..9071
                     /gene="hisIE"
                     /locus_tag="PK768_01410"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HQA59662.1"
                     /translation="MIELDSIKYDEKGLVPVVVQAEDSKEVLMLGYMNKESLQKTIET
                     KKAWFYSRKRKKLWQKGETSGNYLEVKDILYDCDEDTLLIKVIPKGPTCHTGNYSCFY
                     RKLLENVECSAKAEPSNFDIINELVDVIDSRFSERPEGSYIAKLFAGGKERILKKVGE
                     EASEVIIASMSDNRADTIYEAADLLFHLLIALRYDKITVEEVMGELKSRRK"
     gene            9268..9343
                     /locus_tag="PK768_01415"
     tRNA            9268..9343
                     /locus_tag="PK768_01415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9301..9303,aa:Val,seq:tac)
     gene            9352..9428
                     /locus_tag="PK768_01420"
     tRNA            9352..9428
                     /locus_tag="PK768_01420"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9386..9388,aa:Asp,seq:gtc)
     gene            9437..9512
                     /locus_tag="PK768_01425"
     tRNA            9437..9512
                     /locus_tag="PK768_01425"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9470..9472,aa:Phe,seq:gaa)
     gene            9573..9649
                     /locus_tag="PK768_01430"
     tRNA            9573..9649
                     /locus_tag="PK768_01430"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9607..9609,aa:Val,seq:cac)
     gene            9727..9800
                     /locus_tag="PK768_01435"
     tRNA            9727..9800
                     /locus_tag="PK768_01435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9759..9761,aa:Gly,seq:ccc)
     gene            9960..>10948
                     /locus_tag="PK768_01440"
     CDS             9960..>10948
                     /locus_tag="PK768_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQA59663.1"
                     /translation="MSKIFHVGIDIGSTTVKLVVLNRQHEIIYSKYERHYADIRKKLW
                     EILQGAVDIIKDAKATVMITGSGGIGIARDLGVPFTQEVIASTKAIKTYYPKTDVAIE
                     LGGEDAKITYFNDGVDQRMNGTCAGGTGSFIDQMATLMKVDASGLNELARNHKVIYPI
                     AARCGVFAKTDVQPLLNEGVAREDIAASVLQAVVIQTISGLACGRPIRGNIAFLGGPL
                     YFLSELRKRFIETLQLTPEQAIFPENSQLFVALGAALSSLNEQPVHYKTFLAELDAFG
                     KTAIRETNTLEPLFSEPAEYAQFKARHQKNTVKRRDLAGFTGNCYLGIDAGSTT"
BASE COUNT         3360 a         2026 c         2548 g         3014 t
ORIGIN      
        1 tagacaaaat aaaggattac ttaaaagaca aactaggctt ttaggcttac aataagtaag
       61 ggcatcctga ttacaaagga tgcctttaaa atttcattaa caatcaaagg ctttataatg
      121 caggatgcaa gtttgaagga aggttgctaa ttaattgaca caaactatta ttttatagat
      181 gcaaaaattt cttatttgcg gtaatattat agtagaccat ttcacatgcc gcaactttgt
      241 tttattatat aattactatg tgggaggtag tgattatgaa gaaattcatg gatgaggact
      301 ttttgttaag taatgacact gcaaaaaccc tttatcatga gaacgcaaaa caaatgccta
      361 tagtcgatta tcattgccat attgacccga aggatattta cgaagatgta gtttatgcaa
      421 atctcacgca agtatggcta ggaggagacc attacaaatg gcgggcaatg agaaccaacg
      481 gtattgaaga gcgttatata acgggggaag atactacagc atatgaaaaa tttgaaaaat
      541 gggcagaaac cgttcccaag ttgataggca atccattgta tcactggaca catatggagc
      601 tgaaacgtta ctttgatata gatgatattc tttcacctaa aacctgtaag aagatttggg
      661 atgctgccaa tgaacgttta aaggatttgt cagttcgtaa aattatcgaa aagtccaatg
      721 ttaagttgat ttgtacaact gatgatccca tcgatgattt acgctggcac aagtacatat
      781 ctgaagatga gacttgtaaa atcaaagtat taccggcttt caggccggac aaggtattga
      841 acatagataa acccggcttt gtggaatata ttaaaaagct aggggacgtt tgtggtataa
      901 atattaaaac ggttgatgat gttaaagcag ccctggcatc aaggattgag ttttttgtta
      961 aaatgggctg taaagcatca gatcacgcac tggattatat tgtttacaga aagacggacg
     1021 agaatctaga tgccgtgctt caagcagcat tagacgggaa agcaattgac caggaaaaag
     1081 cggatgaata taaagctgag ctcctcatgt tctgtgcatc gcagtataag aaacacgata
     1141 tcgtgatgca gctacattac ggtgcattga gaaatacgaa tcctgcagct tttgcaaagc
     1201 ttggccctga ttctggcttt gatgcaattt gtggctatga taaaagcggt gttgccctcg
     1261 gtgcactttt gggtgcatta gaagccaagg atagtctgcc aaaaacgatc atatattccc
     1321 tcaatcctac agacaatgct ttgatcactg caattatcgg ctgtttccaa tctcctgaag
     1381 tccctatgaa gatacaacac ggaagcgcct ggtggtttaa tgacacaaag accggcatga
     1441 tggaacaaat gactaacctc gccaattttt ctatactgcc caactttata ggtatgctaa
     1501 cagattcacg aagtttctta tcgtacaccc gccatgagta tttcaggcgc atcctctgtg
     1561 acttaatcgg acagtgggta gagaacggcg agtacccggc cgatatggaa attttgggta
     1621 aaatggtgga ggatatctgc tataacaata ccgtcaggta ttttggattt gaagtttaaa
     1681 ctagaaatat tttttcacaa ctgcccaacc tttatcgtga atctctttat aatgtataaa
     1741 ggatgggcag tttttatatg aattactttt gatttgacag aaatatttag gattttccgg
     1801 tgacaccttc acatgggaat atccggaatt tagccattaa atagttttaa aggcaatagg
     1861 gcttcttgca tttctctttt tattttctcc aatccttctt cagttttaga ctcacagcgg
     1921 acgataagct ccggaccggt atttgagctc ctaaccaagc cccaaccatc agagaataga
     1981 acgcgtgcac cgtcaacatc tattataggg tatttgccct caaagtaccg ctttaccgcc
     2041 aggactttgt caaacttttc ttcatcaggg cagggtatgc gaagttccgg tgttgaaaag
     2101 tatttgggca catctgaaag caattctgag agtttgctgt cggtattgga aagtatccga
     2161 aggagcctcg cagcagcata aaaggcatcg tcaaaaccat agtattcatc ggcaaagaac
     2221 atgtggccgg acatttcgcc tgtaaatatt gcacctactt ctctcatctt ggctttgatc
     2281 aaggagtggc ctgttttgta aaatatgggc ttgccaccga gtttttttat ttcatctacc
     2341 agggcttctg agcacttaac ttcaacaatg gcagtagagc caggatattt tggcattatc
     2401 tcacgccaat acagaatcat cagcatatct ccgaatatta cattacctct gtcatcgaca
     2461 accccgatgc gatccccatc accatcaaaa cctatgccca aatcaagttg ttctctcaaa
     2521 actaattctc ttaaatccgc taaattctct gcattaacag ggtccgggaa atgatttgga
     2581 aagctgggat cggaggtgca gtaaagcgga taaacctcca ctccaaggcg gctaaagaat
     2641 ttttcagcaa agagtgaggc agtgccgttg ccgcagtcga tgccgaccct gagttttttg
     2701 gaaaggcgga ttttgctgga tatcatgtcc atataatctt caacggggtc ctttgaactg
     2761 acagaaccct tccctgacaa aaactcaccc ttttccatca tccttcgtaa gtattgaatt
     2821 tcatctccgt atatggttcc agggccaaaa gctactttaa agccattaaa ttgggcagga
     2881 ttgtggctgg cggtaaccat aattcccgga tttataccat agtgttcacg tgcaaagtaa
     2941 aatataggag ttacaactgt cccgatgtcc acgacgtcac aacctgtaga caagagacca
     3001 ttgattacag ccttttttag taagggtgac gaagtcctat tgtcacatcc aactatagcc
     3061 ttgttttcgc cttcttgacg tgcatatgta ccaaaagctt gccccagcct tgtggcaaag
     3121 ctttcatcta aatcttcacc gacaatgcct ctgatgtcgt actgcctaaa aacattggga
     3181 tttaatttca tacaacctgc ctccacatct tattttttaa tgcataataa aatctgttat
     3241 agtatttccc aaaaacctgt aattattttg aatacaacat acaaactacc tttgcactta
     3301 tcagcattat accataaatt ttcattttcc tattgataaa gattaataat tgtgatagta
     3361 ttctcttaaa tgctttaacg atttaatata ctaaaggatg attgttatga ccggctattt
     3421 tagaagacct gcaggagtga aagactattt gccaccaaag gttcgcaaaa agcggcggat
     3481 tgaaaaagta attgtggaca tatttgaaaa gtggggctat gaagaggtaa tagctccttc
     3541 ttttgaatat ttggagacct ttgcgaaaac tggcaagtgg gattttacgg agaagatttt
     3601 taagtttatt gacagacacg gtgaggctgt ggctttaagg cccgatatta ccacatctat
     3661 tgccaggatg gtggcaactc attatagtaa ccatgacgcg cccttaaggt tttattatct
     3721 ggcaaatgtt tttagattcc aggagcccag aaagggcagg gaccaggagt tttatcaaat
     3781 aggcgccgag ctaatcggca taccggggct tgaagccgat gtggaaataa ttcttcttgc
     3841 atcacatatg cttgaacaaa tcggcataaa agacttttta ataaatattg gccacaccaa
     3901 attcctggag ggtctacttg atgaattaga gaacagctcc tctctaaaag acagaattgc
     3961 tcaagccata aaagaaaaaa attttgtgca gcttaaagat ttgattgaag agagtgctat
     4021 aagacctgag atcaaaaaaa cggttctagc tttgccaagg ttttacggtg gagcggagat
     4081 attcgagaaa cttgatcagt ttacattaaa cgaaaaatgc attgcagcac tgtcggaact
     4141 taagaaggtt tggaatatgt taaagcagtt taacaacatc caacttagct ttgatccggg
     4201 gcttgccaga ggcatcgatt attatactgg catcgtgttt gaaatatatt cacctaagtc
     4261 gggatttccc ctgggagggg gagggcgata cgactccctc cttgataagt ttaatggatc
     4321 ccgaccggct acaggttttg cattaaccga ggatgttatt ttgacagtat tagagaagga
     4381 tatggaagat acatacgaac cttattatct ttattacaac ccggcagaat atatcgaggc
     4441 atttcataag gcggaagatt tgagaaggca aggccgcata gtgaaaatgc tgccagctga
     4501 caaatatgat taacatatat tttaaaggag aattgctgta aagatgctaa agattgcttt
     4561 atctaaaggc aggatactat ctgaaaccct aaaacttttt aataaaatac aaatggattt
     4621 tagccaagtg aatgaagaca cacgcaagct tttttttgat tttgaagaat atggcacccg
     4681 tgtaattctg gcgaaaccat cagatgtgcc gacatatgtg gaatacgggg ttgctgatat
     4741 tggggtagcg ggcaaggatg tgcttttaga agataagaaa gatgtttttg agctagttga
     4801 tttaaagctt gctcagtgca aaatggtagt tgcaattcca caagataggt ccatcgatat
     4861 gccgatttat cgggtggcta ccaaatatcc tcaaattgcc gaaagacatt ttgtaaaaag
     4921 gggaattccg gtagaaatca taaaacttca cggttctgtg gaattagggc ctatactggg
     4981 catttcagat gctatagtgg atattgtgtc aactgggagt accctcaaga agaacaattt
     5041 aaaaatcctg gaagaaatat gtcctattag cagcaggctc atagcaaacc aggtcagttt
     5101 ccgggttaaa tcctatgaga taatggctct gatagaaaaa ctggaatctg ttctgtgatt
     5161 agcccttatg taaatggaga aaaaggtgta taattataca tcattagttc aaggaggaga
     5221 ttgacattaa ttcaccaata cgctttgcaa gagacgatgt attaaaaatg ataccttaca
     5281 tgccttcaca agaggcttgc aagataaaac tcgatgccaa cgagagtcct tacgatgtac
     5341 cccttaaggt aaaagaaaga atatggaaga gattgcagga ggagcatttc aactactact
     5401 atgaccccag ttgtgatgag ctgcgggaga gcctttccaa gtacacagga gcaaagccac
     5461 agcaaatatt tgtaggaagc ggtgccgatg aaattattct agacctcatt ttagcctttg
     5521 ctggccccgg tcgagatgtt ataattccat cgcctacttt ttccagttat gaaatctttg
     5581 ccactgtggc aggttctaat gtcataaaag ttcccatcca ccgcataaag caagggaacg
     5641 gctggatttg ggaccttgat ataaatcaaa taaaggagca ttttacgcaa gataaggatc
     5701 agcttttgtt tatctgttat cccaacaatc ctacaggaga ctattttgat gaagaaaaag
     5761 tccttgactt aataaacagc ttcaacggca ttgtggcagt tgatgaggca tattttgagt
     5821 ttggcggcaa gacttttgca gatagactcc atcaatttcc ccacgtagta gttattcgca
     5881 cattttccaa gatattttcc ctggcaggcc ttagagtcgg ttacgccata ggccatgaag
     5941 atttgatcaa tcagctgtat aaagtgaaac tgccctataa tgtaagtctt ttttcccaga
     6001 ttgcagctgt cgaaatttta aaggagacag attggctaga ggatatgaaa aggcagtttg
     6061 caaaagccag agatgaactg aaggaggagt tagataatat aaaagggtta cgagtatatc
     6121 caagtgccgc taatttcttt ctgtgtgaat tagaaaagtc acgggacttt gtatatgagg
     6181 aactcttaaa gaggggaata cttacacgtc gattaagtga tgatatactg aaaaacactt
     6241 tgcgcttttg cataggtaca ccggagcaaa accggtttct tatatcatca cttagagaaa
     6301 ttttggatta aatttggagg aggttctcaa tggctaggac cgctgaagtt tacaggaaaa
     6361 ccggagaaac ggaaataaag gttaaaatga atctggatgg gcaaggggaa aacaaaatca
     6421 ctacacaggt ctttttccta aatcacatgt tagacctttt ttcccgtcac ggcctatttg
     6481 acttagaagt tgatggaaag ggagatatag aagtagattt ccatcattta gtggaagatg
     6541 tgggcatcgt gttgggccag gcttttttga aagccctggg caataaggaa gggattaagc
     6601 ggtacggaac attttatgtc ccaatggatg aagctctggc cttttgcagt gtggatataa
     6661 gcgggagacc ttacatatat tttgatgccg agtttagtcg cgacaaggtg ggggaatttg
     6721 atgtagagct ggtaaaagag ttttttgtag cttttgccaa caatgcccgc ataaccattc
     6781 atttggaagt tgtccgtggg gaaaacaccc atcatatgat agaagcgctc tttaaagcct
     6841 ttggtcgtgc attgcgagag gcagtggcat ttgatccaag ggaaaaaggg gtgccctcca
     6901 cgaaaggggt actgtaagtc tagaaagggt gacggaatga atatatatcc ggcagtagat
     6961 atcaagggag gaaagtgtgt aaggctagtc cagggagatt tttctcagga aaccgtttat
     7021 ttagaaaacc cggtggatgc agcaaagatg tggcaggaga aaggggccaa gtgggttcat
     7081 gtagttgact tggatggtgc aaagtccggg aatcccggca actttgatgt gatccgaaat
     7141 attagagaag ccttaagtgc caaaatccaa gtaggtggcg gcattagaaa tatcggcact
     7201 gttgaaagct atttaaacat gggcatagac cgcatcattt ttggttcttc cgcaatcaca
     7261 gatttgacac tagtgaaggt taccatagac aggttcggag cagaaaaagt ggctgtaggg
     7321 attgatgtta gacaaggtca tgttgctatt gaaggttgga caaaatcatc cggaatcaca
     7381 gtagaagtgg tcctcaggca gttaaaagac attggtgtaa atacgatcat ttataccgac
     7441 atttcaagag atggcatgat gaaagggcca aactttgccg gcatcgagaa agtgttgagt
     7501 ttcggagatt tttcagtgat cgcatctgga gggatttcca gcttggaaga tctggaacaa
     7561 ttgtctatgt atgaaaatag gggtttagaa ggagcaatta ttggaaaagc cctatacaat
     7621 ggttcccttg atttaaagac ggttttagag cgctttgata cttaaggagg ataggagtaa
     7681 taaaaatgaa taaaaaacgg cttatcgtct gcttagatgt acaagatggc agggtagtaa
     7741 agggcattca ttttaaaaac atccgggaca ttggagatcc tgttgagctg gcagcttatt
     7801 atgacagaca gggcatagat gagattgtgc tcctggatat atcagccact tttgaaggca
     7861 gaaagaccac cctagatgta ataaaaagag tttcggagaa tatccgggtg ccttttactg
     7921 taggtggtgg catatccaca ttaaaagata ttgaggattt gcttaaagca ggtgctacta
     7981 aagtttccat cggttctgct gcagtaaaaa atccccaatt aatcaaggaa gctgcccaag
     8041 agtttggcaa cgggcatctg gttcttgcct gtgatgccaa aaaaaacaat ggaagttggg
     8101 aagtctatac tcacggcggc aggacggcca ctggacttga cgctgttttg tggtgcaaaa
     8161 aggctgaaag ccttggcata ggggagatac ttctgacaag tatggatgct gacggcacca
     8221 aggatggcta tgatattcgg ttaacaaggg ccgtagctga tgcagttaaa gtgcctgtaa
     8281 tagcttcggg aggggcagga aagctggaac atttcaagga tgtgtttgtc aaagggcgag
     8341 cgagcggggc acttgccgca tctgtttttc ataaggggat ttttaccgta agggaagtta
     8401 aagaatactt gatttcagaa ggagtagaat aatccatgat tgagttggat agtataaaat
     8461 atgatgaaaa gggccttgtg cctgtagtag ttcaagctga ggatagcaaa gaagtattaa
     8521 tgcttggcta tatgaacaaa gaatcattac aaaaaactat tgagacaaaa aaggcttggt
     8581 tttacagccg caagagaaaa aaactctggc aaaaagggga gacaagcgga aactaccttg
     8641 aggtaaaaga tatcctctac gattgtgatg aagatacgct gcttataaaa gtaattccta
     8701 aaggtccaac atgccacacg ggaaattact catgttttta ccgcaagctt ttagaaaatg
     8761 tagagtgtag cgctaaagct gagcctagca attttgacat aataaatgaa cttgttgatg
     8821 taattgattc taggttttcc gagagacccg aaggatcata tattgcaaaa ctatttgccg
     8881 gcggcaaaga aaggatacta aaaaaagttg gcgaagaagc tagtgaagta attatagctt
     8941 ctatgagcga taacagggct gatactattt acgaagctgc agacctgctt tttcacctgc
     9001 taatagcact tcgatatgat aaaataacag ttgaagaagt aatgggagaa ctcaaaagtc
     9061 gccgaaagta actttcttga caaattgcca ataaatatat ataattttta gtaagcagtg
     9121 agggcaaaga tgttgatgca gcaatagtga actagataaa caaagtaaca acgagttata
     9181 aagtgtataa gtggaaaaat gcttgataat aagtaaatat gatttttgac atcaattcga
     9241 atctaatgta tactagttaa tgtctttggg cggatagctc agctgggaga gcacgtgcct
     9301 tacaagcacg gggtcacagg ttcgagccct gtttcgccca ccaaaatatg cggcccagta
     9361 gttcagttgg ttagaacgcc agcctgtcac gttggaggtc atgggttcaa gtcccatctg
     9421 ggtcgccatt atattagcct tggtagctca gtcggtagag cagaggactg aaaatcctcg
     9481 tgtcggtggt tcgattccgc cccaaggcac cagtctaact ttagaaaaga tcatctcaac
     9541 atgctctgag tcatagagct gacttttacc gggggtgatt agctcagctg gttagagcgc
     9601 atgcctcaca tgcatgaggt cgggggttcg agcccctcat cgcccaccaa tcttttttct
     9661 gcgcttaatt aaatattctc ttatagattt ggtcgtgatt tttaaactgg atgaaaaaga
     9721 tattatgcgg gtgtggttca atggtagaac tccagcttcc caagctggcg gcgagggttc
     9781 gattcccttc acccgctcca ttccgaaaaa agaaagaacc cggcaatagg tcgggtttgt
     9841 tgtttattct ttattgtgaa ataatgaaaa tatattaaaa tagagatgta gtaataattt
     9901 tggcaaggtt tttcttaaaa accaaggagg tgaacacagc gcctttgctg tgggactaaa
     9961 tgagcaaaat ctttcatgtg gggatcgaca ttggttcgac gaccgtgaaa ttagtggtcc
    10021 tcaacagaca gcatgaaatc atctatagca aatatgaacg tcattacgcc gatattcgga
    10081 aaaagctgtg ggagattctg cagggcgcag ttgatatcat caaggatgcg aaagcaaccg
    10141 tgatgattac cggctccggg ggaataggga ttgcccgcga cctgggggtg ccctttaccc
    10201 aggaagtgat tgcctccacc aaagcgatta agacttatta tccgaaaacc gatgtggcca
    10261 ttgaattagg cggagaagac gccaaaatta catactttaa cgatggggtc gaccaacgga
    10321 tgaacggaac ctgtgcagga ggaaccggtt cctttatcga ccaaatggcc acgctcatga
    10381 aggtggacgc cagcggtttg aatgagttgg ccaggaacca taaggtgatt tatcccatcg
    10441 cagcccggtg tggtgttttt gcgaaaaccg atgtgcagcc cctcttgaac gaaggagtgg
    10501 caagggaaga tattgccgcc tcggtgctgc aggcggttgt gatccaaacg atcagcggtt
    10561 tggcctgtgg ccggccgatt cggggcaaca ttgctttttt agggggcccc ttgtattttc
    10621 tatcggaatt aagaaaaagg ttcattgaaa ccttgcaatt aactccggag caagcgatct
    10681 tccccgaaaa ctcgcaactt tttgtggcgc tgggggcggc cttgtcctcg ctgaatgagc
    10741 agccggtcca ttataaaact ttcctggcgg aattggacgc tttcgggaag acggccatca
    10801 gggaaaccaa taccctggaa ccgctctttt cagaaccggc ggaatatgcg caatttaaag
    10861 caaggcatca aaaaaatacc gtcaaaagaa gggacctggc cggttttacc ggtaactgtt
    10921 atttagggat cgatgccggt tcgaccac
//
LOCUS       DAONUO010000021        10851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4670_length_10851_cov_3.470915, whole genome shotgun sequence.
ACCESSION   DAONUO010000021 DAONUO010000000
VERSION     DAONUO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4670_length_10851_cov_3.470915"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..895
                     /locus_tag="PK768_01445"
     CDS             116..895
                     /locus_tag="PK768_01445"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HQA59664.1"
                     /translation="MWTRGYLKDRAKKVLGVSYWKAFLVSIILLLVGANGSGGGSGGS
                     ASNSAETLRGLSKEELIIYLIIIFVVVILALIFGLAFRIFLGYPLEVGGRKFFIENAQ
                     LEGQTSADLNSLGFAFKKDKYMDVIKAMLYKGVLNFLWYLLFIIPGIVKSYAYMMVPY
                     ILAENPNIGYRRALELSEQMTYGHKMDIFVLKLSFIGWYLLGLMCCCIGTVFVVPYEN
                     ATLAELYLVLKYQAIDKGICSYEEFMAMDIIPPRQGFSEFQ"
     gene            910..1359
                     /locus_tag="PK768_01450"
     CDS             910..1359
                     /locus_tag="PK768_01450"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQA59665.1"
                     /translation="MVEILLLLIKVIFIIVIYLFIFGVIRLIYLDIKSMNISKTNTMG
                     FHPYFKLINQRDMLGFKVEENYTLDRSVTIGRQDKNDIVIKDPFISEIHAHVSVEGDM
                     VYIKDLKSKNGVFVNGSRVDGDQRVLLQDGDIIKIGQVKFLFVAAGK"
     gene            1371..2639
                     /locus_tag="PK768_01455"
     CDS             1371..2639
                     /locus_tag="PK768_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQA59666.1"
                     /translation="MLGETTFRRPLLLIAVFNIIGYSFLFLSGEAYNWHIAALGTFVT
                     LLICTVCLFIIKRNAGDEYLFLIVSMLLSIGLLVISRLNEGLGIKQFIWVLVGLTLFF
                     SVYFVYIRVSKWERFTYLYIFLPFAFYLITLVLGKNINGAVNWISIGGFTFQPSELSK
                     ILFIFFLASYFKQPDMLFLKDSRYSEKTKVVVNRVLLMLVTYLNIGFLVIQRELGTAV
                     LMFLVFLIVLYVYGRDMRTFFLNSALIIPGLLASYALFYHIRVRVEVWIDPWSDITDK
                     GYQITQSLFAIASGGFFGTGIGMGRPDMVPAVTTDFIFAAICEELGVFGGVAVVLLFM
                     LLIYREIKIILGVKNRFNKILGFGIAVMFGLQTFIIIGGVIKLIPLTGITLPFVSYGG
                     SSLTTSFIALGIMQAISNTDMDEAGGEIYG"
     gene            2632..4068
                     /locus_tag="PK768_01460"
     CDS             2632..4068
                     /locus_tag="PK768_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA59667.1"
                     /translation="MAENKRIIRVMVGLCSLFLALVVYLTFIQLFKSKSLIANTYNRR
                     QVKIEENTARGNIYDRNGVLLAYSEKNGEQQERIYPFGPLYSHVIGYNSKVYGKSLIE
                     AAYNRELLGINEYSQVFGMASSSNYPGRKGDNIYLTIDHELQALGGELLQGRKGAVVA
                     MDPKTGEVLALVSSPSFNPGAKELEKNWQVMVESQDAPFLSRATQGLYPPGSTFKILI
                     TAAAIEKGLENEIYNDKGSIVIDEREIRNSAGKAYGEIDLKKALAVSSNVVYAQLGVT
                     LGEGSLKDIAQRAKFGKEIDFDIPVSASRFPHDSMSKTDLAESAIGQGKVLVTPLHMA
                     MITSGIANGGVMMKPLLVKKLEDPDGKTDKDAKSAEFSKIMSKDVSEKIKVMMQEVVV
                     SGTGHNAAIQGINVAGKTGTAENELSVKKKAKEHSWFVGFAPVEDPKIAVAVIVEYGG
                     SGSDTAAIIARNIIKKYLLEQKTSENKN"
     gene            4934..7000
                     /locus_tag="PK768_01465"
     CDS             4934..7000
                     /locus_tag="PK768_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQA59668.1"
                     /translation="MFQRIRTRLYKFEIIRKTRVNVRLILAFILLSIIPITLLGVYAY
                     YLSKNAIESKIYSYTEQFTDQIKKSIDGQLAKYERDTFDISTNKEIQQQLRIASGSEN
                     DFDKFKAIKALEDLLMTKFTLTKEISFARFMIDNGETIDYQLTGVLQKEGVNDALVRL
                     ADEGDGAPLWTTVKDDENFYLVCVKVINDLQSAKRLGYLFIGLKNEALVNVYKDVDIG
                     GGSEIFVIDKDGTIISSENEKELGNMYGDEELANEIGAKISEDTNVFEFNDYLVSYKE
                     IEGTNWVVVGKIPISYTRHEPERIRDSLLGFIAVCLILSLLTSIIISMSISVPLERMA
                     KLMNETKNGIFTSDIEDYGKDEISDVIKDFKNMISNISVLISKVKLSSNDVLNHLSVL
                     DTSIEKSNAASKQINGITRQIAADASEQASEINNCAISIRILSEGINKVEDNMQDMTK
                     VIDNTKKLSQDALEIVETLNDKATKTSMASNRVIEDINKLSEDIKRIKAIITTIAGIA
                     EQTNLLSLNAAIESAKAGEAGKGFGVVASEVKALAEQSKEASSKIGKIINDILLKTEE
                     TVNVAYSAHSNVNDQMSVVNQTNESFNSIFNAMESIVGCIDDMSESIKEVLDSKEKVS
                     ASIENIATISEQTASITQEVSATTQEQYSYMESLAAASKKINLMAEQLADAVSKFRIR
                     NEEDTI"
     gene            6997..8319
                     /locus_tag="PK768_01470"
     CDS             6997..8319
                     /locus_tag="PK768_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQA59669.1"
                     /translation="MKKVFYKIVCMVITLVLFLNLSACTNSGKAKDVDDKQNPQTKIE
                     GNNQNKIKLSLWHMWVSDSDGNKKPFQTVLKHVEEKFPNVELVVEGIDNESYKKKLKN
                     AMATNQIPDIFFTWSAGFSKPFVENGKVLALDEYIGDIKDKLLPGITDSMTYGGKVYG
                     LPYQMQVAALYCNKELFDQNGVKIPENYEELLTAVKAFSEKGITPMVNGAKELWPSMF
                     YYDILALRTAGPQLCNDALNKKASFDSPEFIDAARRLVELTKAGAFGENPLGLSYDNG
                     NIMFAEGKAAMLFNGNWVAGIVEGDTSKVKGKIIAVKFPLINDGKGSLTEYLGGAGDG
                     FMVSADTPNKELAVDVAKFIAEDMARESYLANAGMPGWIVQVDDSKVSPMVKQMVNMT
                     QDSTAWILWWDVFLEGEDAEIHKNLVVELIAGQISPEDFAKEMQKLNE"
     gene            complement(8386..10107)
                     /locus_tag="PK768_01475"
     CDS             complement(8386..10107)
                     /locus_tag="PK768_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQA59670.1"
                     /translation="MKNTLKLLISVGILIIFISVSIFVSDAVSSVDYFNEGNSLLNSG
                     KTEEAIQSYNKAIELNSEIPEYHYNKGVALLQLGKYDEAIVSFDSSIELAPKFKEPYI
                     NKIVCLLQTNKAEEALETSDKCLEFYPNDSYTYILKGCALNILKRYEEVLDTSDKVIS
                     LNPSENTVLSYAYSNKGAALAWLRRYEDAFNACNKAIDLYNANTDAYLSMSFAYSLQR
                     NYEKAIDMCDKVFEINPSSLDAYINKSSYLVYAERYEEAVECCNKALEMDVLKSPYIE
                     SSLLANKAGALASLNKAEEALAATEKAIELCPENSLAYANKGSALDILGRYEEALVFS
                     NRAIGLDPTLGYAYSAKGHSLYMLKKYDEAIAACSKAIELNPEDIRICVQCYSNIGAS
                     LNGKGNYEEALENIDKAMEYEINDPNILYSVYVNKAYALTGLQKFEESLEYSNKAIEL
                     NPQDCKGYVNKSSALIKLSKYDEAIECCNTAIESNIEDYLLYNNMGFALEKKGKLKQA
                     LQAYSKSLALSPDNGLVKSNLLRALTWYNIKRVCLFLAIFSVIAAIIIIAVVQIIKKK
                     KSQTITP"
     gene            10630..>10851
                     /locus_tag="PK768_01480"
     CDS             10630..>10851
                     /locus_tag="PK768_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59671.1"
                     /translation="MGKKAILCMLLAMSLIIMGIPSLQYPAKAAYIELKGTDIYNGLR
                     GQSFNEGWKFNKGDASNGQSINYDDSKWSD"
BASE COUNT         3463 a         1742 c         2322 g         3324 t
ORIGIN      
        1 gtcttttacg ttttattgtg aaaatcattt attcttgata taatttaaca ttataaaaat
       61 atataatctg tattggtaat gaggctgggt aacggctgaa gaacaggggg atattatgtg
      121 gactagagga tatcttaagg atagggcaaa aaaagtgtta ggggtttcat attggaaggc
      181 ttttttggta agtatcattt tgctcttagt cggtgcaaat gggagcggag gaggcagcgg
      241 aggaagtgct tcaaacagcg ctgaaacttt aaggggcctt tcaaaggaag aattaataat
      301 ttatttaata ataatatttg ttgttgttat actagcccta atatttggtc tggcatttcg
      361 aattttttta ggatatcccc ttgaggttgg aggaagaaag ttttttattg agaacgcaca
      421 attggaaggg caaacttctg cagatttgaa ttctcttggc tttgcattta aaaaagataa
      481 gtacatggat gttataaaag caatgctgta taaaggagta ttgaattttt tgtggtattt
      541 attatttata atacctggca ttgtgaagtc ttatgcatat atgatggttc cgtatattct
      601 ggctgagaat cctaatatag gctatagacg ggctttggag ttaagtgagc aaatgactta
      661 tggacataaa atggatattt ttgtattgaa actctcgttt attgggtggt atttattggg
      721 tctcatgtgc tgctgtatag gtactgtttt tgtagttcct tatgaaaatg ctactttggc
      781 agagctgtat ttggtgctga agtatcaggc cattgacaag ggcatttgca gctatgaaga
      841 gtttatggcg atggatataa taccgccgcg tcaagggttt agtgagtttc aatagaggat
      901 ggcataaaaa tggttgagat attactgtta cttataaaag ttatttttat aatagttata
      961 tatctgttta tttttggtgt cataagactt atttatcttg atattaagtc tatgaacatc
     1021 agcaaaacca ataccatggg ttttcatccc tattttaagc ttattaatca acgggacatg
     1081 ctggggttta aggttgagga aaactacact ttggatagaa gtgttactat aggccggcag
     1141 gataaaaatg atattgtaat aaaggatccc tttatttccg aaattcatgc ccatgtttca
     1201 gtggaaggtg atatggttta cataaaggat ctaaagagca aaaatggtgt ttttgttaat
     1261 ggatcaaggg ttgacggaga tcagagagta ttattgcaag acggcgatat cattaagatc
     1321 gggcaggtaa aatttttgtt tgttgctgcc ggtaaatgag gtgtttatta atgctggggg
     1381 aaacaacatt ccgcagacct ttgcttttaa tagctgtgtt taatattatt ggatattctt
     1441 ttcttttttt aagcggtgaa gcttacaact ggcatattgc tgcactggga acttttgtaa
     1501 cccttctgat ttgcaccgtg tgccttttta ttatcaagag gaacgcggga gatgagtatc
     1561 ttttcttaat agtatccatg cttttaagta ttggactttt ggtgatatca cggcttaacg
     1621 aagggctggg cataaaacag tttatttggg ttttagttgg tttaacactt tttttctccg
     1681 tttattttgt atatatacgg gtgagcaaat gggaaagatt tacctacctg tatatattct
     1741 tgccttttgc attctatctc attaccttgg tcttggggaa aaacatcaat ggtgcggtaa
     1801 attggatttc catcggcggg tttacctttc agccatcgga attgagcaaa attcttttta
     1861 ttttttttct ggcaagctat tttaaacaac cggatatgtt attcttaaaa gattcccgat
     1921 acagcgaaaa aaccaaagta gttgtcaatc gagtgctttt aatgcttgtc acatatttga
     1981 atattggttt tcttgttatt caaagggaat tgggaacagc ggtcctgatg tttttggttt
     2041 ttcttatcgt cctatatgta tatggcaggg acatgagaac ctttttcttg aattcggcac
     2101 tgataatacc gggacttctg gcaagttatg ccctctttta tcacattaga gtaagggtgg
     2161 aggtctggat tgatccgtgg tcggatatca ccgataaggg ttatcagata acccaatctt
     2221 tattcgccat tgcttcaggt gggttttttg gaacgggaat cggtatggga agacccgaca
     2281 tggttccggc agtgacaacc gactttattt tcgctgcaat atgtgaagaa cttggggttt
     2341 ttggaggagt tgcggtagtt cttttgttta tgcttcttat ttatagagag ataaaaataa
     2401 tactgggtgt gaaaaacaga tttaataaga ttttgggttt tggaattgct gtaatgtttg
     2461 gattgcagac ctttattatc atagggggag tgataaagct aatacccctt acgggaataa
     2521 cccttccttt tgtgagctat ggaggaagtt ctcttaccac cagctttatt gctctgggta
     2581 ttatgcaagc tatatcaaat acggacatgg acgaggcagg cggtgaaatt tatggctgaa
     2641 aacaaaagaa taattcgggt tatggttggt ctatgttctc ttttcttagc gttagttgtt
     2701 tacctcacat ttattcagtt gtttaaaagt aaaagcctta ttgccaatac ctataacagg
     2761 cggcaggtta aaattgaaga gaacacagcc cggggcaaca tatatgacag aaacggagtt
     2821 cttcttgcat acagtgaaaa aaacggagaa cagcaggaga gaatatatcc ctttggacca
     2881 ttgtacagcc atgtgatcgg gtacaactca aaagtgtacg gtaaatcctt gattgaagcg
     2941 gcttataaca gagagctgtt gggaataaac gaatacagtc aggtatttgg aatggccagt
     3001 agttcaaatt accctggcag gaaaggggat aatatttatc ttaccattga ccatgaactg
     3061 caagctcttg ggggtgaatt gctccagggg agaaagggtg ctgttgtggc tatggatccc
     3121 aaaacgggag aagttcttgc tttggtaagc agtcccagtt ttaaccctgg ggccaaagaa
     3181 ttggaaaaga attggcaggt tatggttgaa tcccaggatg cacctttttt gtcccgggct
     3241 acgcagggcc tttatccccc aggctctact tttaaaattc tcattactgc cgcagcgatt
     3301 gaaaagggat tggaaaatga aatttataat gacaagggat ctatagttat tgatgaaagg
     3361 gagatcagga attcagcagg aaaagcttat ggagagatag atttaaaaaa ggcccttgcg
     3421 gtatcgagca atgtggtata tgcccagctt ggtgttaccc ttggcgaggg aagcttaaag
     3481 gatattgctc aaagggcaaa attcggtaaa gagatcgatt tcgacattcc agtaagtgcc
     3541 agccgctttc cgcacgacag catgagtaaa acggatttgg ctgaatcggc tatcggacag
     3601 ggaaaggttt tggtaactcc tttgcacatg gcgatgatca catcgggaat tgccaacgga
     3661 ggtgtaatga tgaagccttt gttggtgaaa aaactggaag accctgacgg taaaaccgat
     3721 aaagatgcaa aatcagcaga gtttagtaaa ataatgtcaa aggatgtttc tgaaaaaatt
     3781 aaagtaatga tgcaggaggt agtggttagc ggtacagggc ataacgcggc aattcagggc
     3841 attaatgtag ccggtaaaac aggaacggca gaaaacgagt tgtcggtgaa gaaaaaggcg
     3901 aaggagcatt catggtttgt cggttttgct ccggtagaag accctaaaat agctgttgct
     3961 gtaattgttg aatatggtgg ttcaggaagt gacactgcag caatcattgc aaggaatata
     4021 ataaaaaagt acctcttgga gcaaaaaact tcagaaaata aaaactgaaa gaatatttac
     4081 ataaaagtag agcccatgat gatctacact acagctgata taaatctttg catacctata
     4141 aacaatcttt cgactgccca caagcattac aaaacctata ccaacctttg ctcgattatc
     4201 aaggatacct tagaaacctg ttgcaatcat ccatagactg cagcaaacct ccagcatata
     4261 atacctttgc acagggttta ccgagtcaat cggtaagcta atgcaataga attaatgcct
     4321 gcggaaccct tgatagtctt agctcgagtg ctatacatcc ctttcaaccg tattgtaata
     4381 actattggga tgattcacaa aactgcatga aaaatccatt cattgttact acaatacagc
     4441 tttacggaac atatagcatt cttccctccg gctaatatca agccttagta ccaacaattg
     4501 gatagacctg gtatcaacct ttggccgaac actataaagc cacctagtga ccttatattg
     4561 ttcttggctc gaccatcatg aaccctacgc taattattat ggaccgaatt caaattatga
     4621 ttcgtggtaa aatttagaat ttaaaaaagc cttcccctaa gaaagtttat tatatacatc
     4681 tcaaaataat aaatgtaagt aagtagaagt catgcaaaaa aaaacatata attgcatcag
     4741 agggtttttc cgaaatataa aagagagact tttgatcgat ataatgccgg gattcaatcc
     4801 aatgagactt tggattgtct tttgtgacat gtgtttcatt atagttacgt atttcgttgt
     4861 tttatgattc attcagaact ctttaattgg gtcgattgag aaatccaata agatattttg
     4921 aggagaaata tttatgtttc aaagaatacg gacaagactc tataaatttg aaattatcag
     4981 aaaaaccaga gttaatgtgc gtttaatatt ggcttttata ttactttcaa tcattccgat
     5041 tacattactt ggggtgtatg cttattatct gtcgaaaaat gctattgaat ctaaaattta
     5101 cagctacact gagcagttta cggaccagat taaaaagagt attgacggac agttggcgaa
     5161 atatgagcga gatacttttg atatttccac caataaagag attcaacagc aattgaggat
     5221 agcatcgggc tcggaaaatg attttgataa attcaaggcg ataaaggctt tggaagattt
     5281 gctgatgaca aaatttacac ttactaaaga aatttctttt gcacgattta tgattgacaa
     5341 tggtgaaact atagattacc aattgacagg tgttttgcaa aaagaaggtg ttaatgacgc
     5401 gttggtccgg ttagccgatg aaggagacgg agcacctctt tggacaactg tgaaagatga
     5461 tgaaaacttt tatttggttt gtgtaaaagt gataaatgat ttacaatcgg caaaaaggct
     5521 gggatacttg tttatcgggc tgaaaaacga agcattggtt aatgtctata aagatgttga
     5581 cataggagga ggttcggaga tttttgttat tgataaagac ggaacgatta tttcatctga
     5641 aaatgagaaa gaactcggaa atatgtacgg tgacgaagaa cttgcgaatg aaatcggtgc
     5701 aaagataagt gaggatacaa atgtatttga gtttaatgat tacttggtgt cgtataaaga
     5761 aattgaagga acaaattggg ttgtggtcgg taaaattcct atatcatata ccagacatga
     5821 gcctgagagg atcagagatt cactgctcgg atttatcgca gtatgcttga tactttcctt
     5881 attaacatcg attattatat cgatgagtat ttccgtacct ctcgaaagaa tggccaaact
     5941 tatgaatgag actaaaaacg gtatttttac atccgatatt gaggactatg gaaaggatga
     6001 aatttcggac gttataaaag actttaagaa catgatatcg aatataagtg ttcttatatc
     6061 caaggttaag ttatcatcca atgatgtttt gaatcatttg tccgtattgg acacttcaat
     6121 tgaaaaatcc aatgctgctt caaagcaaat aaacggtatt acccgacaaa tagctgccga
     6181 tgcatcggaa caggcttctg aaataaacaa ctgtgcaata agtatcagga tcctttcaga
     6241 aggcattaac aaggttgagg ataacatgca ggacatgacg aaggtaatag ataacaccaa
     6301 aaaactcagc caggatgccc tggaaatcgt cgaaacatta aatgataaag ctacaaagac
     6361 aagtatggca tcaaacagag taatagagga tatcaataaa cttagtgagg atataaaaag
     6421 aataaaagct ataattacaa ccattgcggg aattgcagag cagactaatc tcctttcttt
     6481 aaatgctgct atagaatctg caaaggcagg agaggcaggg aagggtttcg gagtggttgc
     6541 aagcgaagtt aaagcacttg ccgagcagtc aaaagaagct tccagcaaga ttggtaaaat
     6601 tataaacgat attttgttga aaacggaaga gacggtaaat gtagcttaca gcgcacacag
     6661 caatgtaaat gatcaaatgt cggtggttaa ccaaaccaat gaatcgttta attccatatt
     6721 taatgcaatg gaaagtatag taggttgtat agacgatatg tcggagtcga ttaaagaagt
     6781 gcttgattca aaagaaaagg tttcggcatc catagagaat atagctacaa tttcagagca
     6841 gacagcatca attacacaag aggtatctgc aacaactcaa gaacaatact catatatgga
     6901 atcgttggct gcggcttcta aaaaaataaa tctgatggca gaacaattag cggatgcagt
     6961 atcaaagttc agaataagga acgaggagga tacaatatga aaaaagtctt ttataaaata
     7021 gtttgtatgg tgattacact ggttttattt cttaatttgt ccgcatgcac aaacagtgga
     7081 aaagccaaag atgttgatga taaacaaaat cctcaaacaa agatcgaagg caataatcag
     7141 aataaaataa agctcagcct ttggcatatg tgggtttccg acagtgatgg aaataaaaag
     7201 cctttccaaa cggtattgaa acatgttgaa gaaaagtttc caaatgtcga gcttgtggta
     7261 gagggtattg ataacgagtc ttacaagaaa aaactaaaaa atgctatggc aacgaatcag
     7321 attcctgaca ttttctttac ttggtccgcc ggtttttcga aaccgtttgt tgagaatgga
     7381 aaagtgttgg ctcttgatga atacattgga gatattaagg acaagctatt gccaggaata
     7441 acagacagta tgacatatgg cggcaaggta tacggattgc cgtaccagat gcaggttgct
     7501 gctctatact gtaataagga actttttgat cagaacggag ttaaaatacc ggaaaactat
     7561 gaagagctgc taacagcggt aaaagcattt tccgaaaaag gaattactcc tatggtaaac
     7621 ggagctaaag aattgtggcc ttcaatgttt tattatgata tcttggctct tcgaacagcg
     7681 gggccacaat tgtgtaatga tgcattgaac aaaaaagcat cctttgattc tcctgaattt
     7741 attgatgcgg caagaagact ggtcgaatta acaaaagccg gtgctttcgg tgaaaatccc
     7801 cttggattgt cctacgacaa cggtaacata atgttcgcag aaggaaaagc tgcgatgctg
     7861 tttaacggaa actgggtggc cggtatagtt gaaggtgata cctcaaaagt caagggtaag
     7921 attattgcgg ttaaattccc gttgatcaac gatggaaaag gaagtttgac ggagtatttg
     7981 ggaggtgccg gtgacggatt tatggttagt gccgacacac ctaataagga actggctgtt
     8041 gatgtggcaa agtttatagc agaagatatg gcaagagagt cttatcttgc aaacgcaggt
     8101 atgccggggt ggattgtgca ggtggacgat tcgaaagtat caccgatggt taagcaaatg
     8161 gtgaacatga cacaggattc aacggcgtgg attctttggt gggatgtatt cctggaaggt
     8221 gaagatgctg aaatacataa gaatcttgtg gttgaattga tagccggaca aatatcaccg
     8281 gaggattttg caaaggaaat gcaaaaactc aacgaatgac acaaaatgat gacacggggg
     8341 gacggtttgg tgtgatctta aaccgtcccc ctatgcgttt gtatttcatg gtgtgatggt
     8401 ttgagacttt ttctttttta tgatctggac aacggctata ataataattg cagcaataac
     8461 cgaaaatatt gccaaaaaga gacaaactct tttaatatta taccaggtta aggctcgtag
     8521 aagattactt tttacaagtc cattatccgg actgagcgcc agacttttgg aatatgcctg
     8581 caaagcttgt ttaagcttgc ccttcttttc aagagcaaaa cccatattat tataaagcaa
     8641 ataatcctct atattcgatt cgatagcagt gttgcaacac tcaatagcct catcatattt
     8701 tgaaagcttt ataagagcag agcttttatt tacatatcct ttacaatcct gagggttcaa
     8761 ctctattgct ttgttgctgt attcaagaga ttcttcgaat ttctgaagtc ctgttaaagc
     8821 atatgcctta tttacataca cagagtacaa aatatttgga tcgtttattt catattccat
     8881 ggctttatca atattttcca atgcctcttc ataatttccc tttccattta aagaagctcc
     8941 gatatttgaa taacactgaa cacatattct gatgtcttcg ggattcaact cgatggcttt
     9001 actgcatgca gctattgcct catcgtattt ttttagcata tacaacgaat gacctttagc
     9061 actataggca tagccaagtg ttggatccag gccaatagcc ctattgctga aaacaagggc
     9121 ttcttcatat ctaccgagaa tatctaatgc actgccttta ttagcatatg caagggaatt
     9181 ctcgggacac aactctatag ctttttctgt agcagcaagg gcctcttcag ctttattgag
     9241 agatgccaac gcgcctgcct tattggcaag taagctggat tctatatatg ggctttttaa
     9301 aacatccatt tccaatgctt tattgcagca ctcaacagcc tcttcatatc gttctgcata
     9361 taccaaataa gaagatttgt ttatatatgc gtcaagagaa gaaggattta tctcaaaaac
     9421 cttatcgcac atatcaatgg ctttttcata gtttctttgt aaactgtaag caaaactcat
     9481 ggacaaataa gcatcggtat tggcattgta gagatctatt gctttattgc acgcattgaa
     9541 agcatcttca tatcttctaa gccaggccag ggcggcacct ttattggaat atgcatagga
     9601 aagaacggta ttttcagagg gatttaagga aatcactttg tccgatgtat ccaaaacctc
     9661 ttcgtaacgc ttcagaatat ttagagcgca tccttttaat atatatgtat atgagtcatt
     9721 cggataaaat tccagacatt tatctgaggt ttcaagtgct tcttccgctt tattggtttg
     9781 cagaagacat acgattttat ttatataagg ttctttaaat ttaggggcca attctatcga
     9841 actgtcaaaa gatactatgg cttcatcata ttttccaagc tgcaataaag caactccttt
     9901 attatagtga tactcgggaa tttcggaatt caattcaatg gccttattat agctttgtat
     9961 tgcttcttcc gttttgccgg aattcagaag agaatttcct tcattaaaat agtcaacaga
    10021 cgaaactgcg tccgatacga aaatagaaac ggaaataaaa ataataagta ttcccactga
    10081 aattaatagt tttaatgtgt ttttcataat attttccttt tctctttctt atgaaaatat
    10141 tatattcatg gtatacatat acgtttatta acttttacta catggtttac tataattgat
    10201 aggtgatatt acctataaat actaacttta ctacaaaaag tgtagtgctt aaggctaatt
    10261 ataccataat ctttaatagc cattcaaggg cacaagagaa caggggaacg tttcttctgt
    10321 gcagaacact tcagcacaga agaactgtcc ccctgtgtca tttccataaa agccattcct
    10381 ctattattta ttttataaaa tccttttaaa ttcccaactt aaaaagacca gtactttcgt
    10441 aaaagtaaaa caaggggtag ttataaaact ttttatatat aatataaaaa ctcattaaat
    10501 atcatggcac tttgcgtttc aggtaaaagg attgaatatc gtttggaatt tctatatcag
    10561 tgtccctgtg aaatcataag tgaataaata taatgactat aaataattat ttttaaggga
    10621 ggagattgta tgggaaaaaa agcaattcta tgtatgctcc tggcaatgtc attaattata
    10681 atgggcattc ctagtttgca gtatccggca aaggctgctt acatagaatt aaaagggacg
    10741 gatatttaca acggactaag aggtcaaagc tttaatgaag gctggaagtt taataaaggg
    10801 gatgccagca atggtcaaag catcaattat gatgacagta agtggtcgga t
//
LOCUS       DAONUO010000022        10837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4677_length_10837_cov_4.144315, whole genome shotgun sequence.
ACCESSION   DAONUO010000022 DAONUO010000000
VERSION     DAONUO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10837
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_10837_cov_4.144315"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1686)
                     /locus_tag="PK768_01485"
     CDS             complement(1..1686)
                     /locus_tag="PK768_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HQA59672.1"
                     /translation="MIVKPAKTLKISAFIIFFIIFISCVFFFLNFYTVSAFPEDKAAL
                     IIENTVITEGTPFILENDRVLLSYNIIKEHLDPYIFWDAAENKITITTLDKTVRLATD
                     SLTAFINTKPVEISFPAKNVTTQPYIPIDFLEELYDIKVKLIGEKNRVIIDKNLSPRK
                     IGTAKSSAVTVKLSPTWFSAPVARLTKTDTLTVYSLEEGWFQVRTEEGLIGYVPEHKL
                     EVKEMPKKAENAPQARQHNPGRGKLNMVWDYIHRVTPDMSGEKAPLGLDIISPTWFSI
                     VDGQGTIESKADISYIRWAHNNNLAVWALINNDFDPDITREFLSSSETRDKIIRQILM
                     YAELFQLDGINIDFENVYYEDRDLLVQFVRELTPILHEAGIVVSMDVTPKSTSLNWSL
                     CYDRKELGKVVDYMILMAYDEHWATSPVSGSVASIGWVEKGIVSLLEDVPAEKVILGL
                     PFYTREWEERPAENGGIAVKSRALSMAQAKRIISQNGSSLSWDEKAGQHFAYYTKGDK
                     VYKIWLEDDKSIQLKAALVNKYDLAGAASWRKGFEEPIIWEVLYNELKLPFKV"
     gene            1903..4089
                     /locus_tag="PK768_01490"
     CDS             1903..4089
                     /locus_tag="PK768_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HQA59673.1"
                     /translation="MVELEGTVERITYYNQENGFTVAKLAVKDDEDLITAIGYFHSLE
                     VGEVLKLKGSWTLHKDYGHQFKVDYYETLIPATSKEIENYLASGLIRGIGPATAKKIV
                     NMFGEKSLEVLANSPGELLRIPGIGEKKLEMIRESYSQQQETREIIMFLQKYGVGPGI
                     AVKIYKNYKDKSIQILKENPYRLADEVFGIGFKTADHIAQSMGMQKDSLERLCAGLKY
                     AMFRAAEEGHVCLPVDELLYRASQLLDVEIELLKQALLALKEKKDIITDCSWGSEDVY
                     LTSFHRCESAVARRLFLLAAMVDKTLDISDEDIDKIEKRCNIKLAARQREALEKAAQH
                     GVVVITGGPGTGKTTTIRSLLEFFKGHDLKVALAAPTGRAAKRMMEATGMEAKTIHRL
                     LEYKAYEEGGMAFGKDHNNPLDEDVIIIDEVSMVDIILMQHLLSAVRLGARLILVGDK
                     DQLPSVGPGSVLKEIIEAERLPVVVLDEIFRQAQESMIVVNAHRINKGYFPYLNVKDK
                     DFFFEQVLLPEDILQTILELVTTRLPKYKNFDPMEDIQVLTPMKKGLLGVHNLNNSLQ
                     GLLNPPKPGKREVHFRSQIFREGDKVMQIKNNYEKEVFNGDLGRIVKIDEEQVLVTFA
                     DSWQERDIVYQLQELEELNLSYALSVHKSQGSEFPVVIMPISTQHYVMLQRNLLYTAV
                     TRAKKLLVLVGTKQALTIAVQNNKSIRRYGHLSDRIICEFSQAKSV"
     gene            4179..5012
                     /gene="larE"
                     /locus_tag="PK768_01495"
     CDS             4179..5012
                     /gene="larE"
                     /locus_tag="PK768_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HQA59674.1"
                     /translation="MLDEKLSKLKESIKALGKVVVAFSGGVDSTFLLKTCIDELGRDN
                     VLAVTAHSQAITERELIEAQALAALLNARQVIVESTELDDPNITDNPPERCYYCKKQI
                     FSNFTAIARENGYNFILEGTNFSDSDDFRPGMAAIKELGVLSPLKDAGLTKDEIRELS
                     KAMGLPTWNKPSYACLFSRFPYGEKITPDKLKRVAKAEDILRQYGFCQFRVRSHGDLA
                     RIEFLPQEMDKVFDESFRNTVISEFKKIGFIYITLDLAGYRSGSMNEGLKEEDMALWK
                     N"
     gene            5000..5755
                     /gene="larB"
                     /locus_tag="PK768_01500"
     CDS             5000..5755
                     /gene="larB"
                     /locus_tag="PK768_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HQA59675.1"
                     /translation="MEKLRHLLEKLKAGEVAVDEVLEALKNLPYENLGFACIDNHRSL
                     RRGVPEVIYCAGKTPSQVAEITRHMLRNGSNVMGTRADMATFDKVREVAEDAKYYDVA
                     RIFAVQREEQPKNKGKIAVVTAGTSDIPVAEEAAVTAELFGNPVERLYDVGVAGIHRL
                     LMNMEVLRQARVIIVVAGMEGALASVVGGLVDKPVIAVPTSVGYGAHFNGLAALLAML
                     NSCASGISVVNIDNGFGAAYAAHQINRLCENNN"
     gene            5772..6983
                     /gene="larC"
                     /locus_tag="PK768_01505"
     CDS             5772..6983
                     /gene="larC"
                     /locus_tag="PK768_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HQA59676.1"
                     /translation="MSTIKSSTEKNILYLDCFSGISGDMFLGAMLDLGLDEKEFLAEL
                     KKLPLKDYEVEIKKSIKKGITGTDVTVITNEHHPHRGLKEIYEIIDNSSLNSNVKEKS
                     KEAFYKLASVEGKIHGKSPYEIHFHEVGAVDSIVDIVGACILMDMLGPSRVCASAINV
                     GFGTVKCAHGILPVPAPATMELLKGIPVYSGEEPGEFTTPTGALILSTFVEKFGTIPP
                     GIPEKSGYGLGKAEHKSPNVLRAVLIKSLDNSADIHSRGLEEDQVAFLEANVDDMNPE
                     LYEHVIESLFQKGALDVFLTPVIMKKSRPGIKISCICTQDKKLLLSETILRETTTLGV
                     RELEVARLKLSRTIEKASTPFGDIRIKVSKIGDEIVRVTPEYEDMKKLAKKNNMPLLK
                     AYEEIKPFCKK"
     gene            7110..8588
                     /gene="gltX"
                     /locus_tag="PK768_01510"
     CDS             7110..8588
                     /gene="gltX"
                     /locus_tag="PK768_01510"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQA59677.1"
                     /translation="MPDKVRVRFAPSPTGSLHIGGARTALFNLLFARHNNGTFVLRIE
                     DTDTERSTEESAAQIIRSMRWLDLDWDEGPEKGGDFGPYFQSQRLELYKKEVDRLLAE
                     GKAYRCYCTPEELAERREAALKAGKPPKYDGTCRNLTAEQIEKYEAEGRKYAIRLKMP
                     EEGQTEVNDLIRGRVVFENSVLDDFIIVKSNGIPTYNFACVIDDNAMQITHIIRAEEH
                     LSNTPKQIQAYLALGYEIPQFAHVPMILAPDRSKLSKRHGATSVEEFREQGYLAKAII
                     NYLTLLGWSPEGTEEIFEIDKAIKEFTLERVNKTAAIYDVKKLTWINGHYMRELDSAF
                     VTEETIPFMIKKGIITEQEAKEKFDYIRRIVEISRDRAKTLDELADAIAFFFKDVSEY
                     EEKGVRKHFTKENAAKLLTLGADALERLDDFTLEKTEATFRSITEELGLKAAEIIHPT
                     RLAITGRTIGPGLFDIIVLLGREKTVERMRRAVKWISENVES"
     gene            8729..10237
                     /locus_tag="PK768_01515"
     CDS             8729..10237
                     /locus_tag="PK768_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HQA59678.1"
                     /translation="MFGELVGVVISIFFVAIVLVLSELIRRLGKFSTEFTRKFVHIGV
                     SHWWLVAMFLIGDIRYALIPPVIFVFLNYYSYKKELIKSMERGKDSSDLGTVYFPISL
                     IVIIMLTWNGGFLGEDLKHLGALGVLTMGYGDGFAAIIGENFGRRKYEIFGNRKSLEG
                     SIAMFGFAFLVSAIILGNNISFEFDVFRISFVIAVLAAIIEAITPYGFDNLTVPIFTT
                     LNAYYFVKGSPAFLFIYMACIGFVISFFIAYAAYKKESLTLDGSIGATFLGTLMYATS
                     GLFGSIMMVVFFLSSSILSHFKKNKKGKAARQFDKTGRRVIMQVFANGGVGLIHSILY
                     YITKNPAYLVFLGVSFAAANADTWATELGLLNKKNPISLRTFKPVEKGTSGAISLFGT
                     FSALIGSMLIGIFATFGFSLLNINIAELGLEVTHAFQMVTLGGFIGSLIDSILGATVQ
                     GVYYSHELKGETEKKEYNGRPNLLIRGFGFVNNDLVNFLSIGISSILFLGIA"
     gene            10370..>10837
                     /locus_tag="PK768_01520"
     CDS             10370..>10837
                     /locus_tag="PK768_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59679.1"
                     /translation="MNFFRLALFGVFLLLVVLLVPLVAGEQGQLIHAVEGAKEVVNGE
                     VVEEPSPVDEPANNEAVEEKPYPIPPEKQEEEALFPRNMTVLNASEITAVNIRERPTA
                     DSKSIGIVYGNLMHVDVIQNLDNGYSEISTWDYASMKDIVGFVPTELLKTVELN"
BASE COUNT         3178 a         2133 c         2620 g         2906 t
ORIGIN      
        1 ttaaacttta aaaggcagct ttagctcatt atacagcact tcccagatga tcggttcttc
       61 aaaacctttg cgccaggaag cggcccctgc caaatcatat ttatttacaa gggctgcctt
      121 cagctgaatg gatttgtcgt cttccagcca tattttgtaa accttgtcac cttttgtata
      181 gtaggcgaaa tgttgccctg ctttttcatc ccagctcagg ctgctgccat tttggctaat
      241 tattcttttc gcctgggcca tggataaggc cctggatttt acagcaatgc cgccattttc
      301 agcaggcctc tcttcccatt cgcgggtgta aaaaggtaag cctagtatga ctttttcagc
      361 cggcacatct tccaataggc ttacaatgcc tttctctacc cagcctatag aggccacgga
      421 gccgctgacg gggcttgttg cccaatgctc atcataggcc attaaaatca tgtaatctac
      481 aaccttaccc agttcctttc tgtcataaca aagtgaccaa ttgaggctgg tggacttggg
      541 tgtaacgtcc atggaaacaa caatgccggc ttcgtgaaga atgggagtaa gttcacggac
      601 gaattggacc aagaggtccc tgtcctcata gtatacattt tcaaagtcga tattgatgcc
      661 gtccagttga aagagctccg catacattaa gatctgcctg atgattttat cccgggtttc
      721 ggagcttgat aaaaactcac gggtgatatc cgggtcaaag tcattgttga ttaatgccca
      781 gacggccaag ttgttgttat gggcccagcg gatgtaagat atatcggctt tgctctcgat
      841 ggtgccctgg ccgtcaacta tggagaacca ggtaggggat attatatcaa gacctaaggg
      901 ggccttttca ccactcatgt ccggtgtcac tctgtggata tagtcccaca ccatattgag
      961 cttcccccga ccgggattgt gctgccttgc ttgaggagca ttttcggctt tttttggcat
     1021 ttctttaacc tccagcttgt gctcggggac gtagccgatt aaaccctctt cggttcttac
     1081 ttgaaaccag ccctcttcaa gggagtacac cgtaagggta tccgtttttg taagccttgc
     1141 tacgggagcg gaaaaccaag ttggtgaaag ctttacggtt acggcactag attttgccgt
     1201 gccgattttt ctaggtgaca gattcttatc gatgatgacc cggtttttct cgccgattag
     1261 tttaaccttt atgtcgtaca gttcctctag aaaatcaata ggaatgtagg gctgtgtcgt
     1321 tacatttttt gctggaaagg atatctccac aggttttgta tttataaagg cggttaggct
     1381 atccgttgcc agacgcaccg tcttatcaag ggtagttatg gtaatcttgt tttcggctgc
     1441 atcccagaag atataaggat ccaggtgttc ctttattata ttatatgaca ggagaacccg
     1501 atcgttctcg aggataaatg gagttccctc cgtaatcacg gtgttttcga taattaaagc
     1561 agctttatct tccgggaagg cggatactgt gtagaagttt aaaaagaaga aaacacagga
     1621 gataaagatt ataaaaaata taatgaaagc tgatattttt aatgtcttgg caggtttgac
     1681 gatcataatt tcctccgtaa ttgggcggtc aatggccgcc tgcttccata taatagcatg
     1741 tctacaaaat ccctgaactg atatatttcg acataaaagc tcaagttcct ctatttatta
     1801 aagtccaatt tttgcggaca acaataatag cgtttaagtt gcaggggaat tcaaaaaatg
     1861 ttagaataaa ccgaaaaggc gatgtaatta gaggtgctgt atatggttga gctggagggc
     1921 accgtagaga ggattacata ttacaaccag gaaaacggct ttacggtggc aaagcttgca
     1981 gtaaaagatg atgaagattt gataacggcg atagggtact ttcattccct tgaagtggga
     2041 gaagtcctaa agctcaaggg aagctggacc ttgcacaagg actacgggca ccaatttaaa
     2101 gtggattatt acgagaccct gatacctgca acatccaaag agatagaaaa ttacctggcc
     2161 tccgggctaa ttcgcggaat aggtcccgca accgctaaga aaattgtaaa catgtttggt
     2221 gagaaatcct tggaagtatt ggctaactca cccggggagc ttttaagaat tcccggcatt
     2281 ggcgaaaaga agcttgaaat gataagggaa tcttatagcc agcagcagga gactcgcgaa
     2341 atcattatgt ttctccaaaa atacggggtc ggcccgggta ttgctgtgaa aatatacaag
     2401 aattacaaag acaaatccat tcaaatctta aaggaaaatc cctatagatt ggccgatgag
     2461 gtgttcggaa ttggctttaa gaccgcggac cacatagctc aaagcatggg aatgcaaaag
     2521 gattccctag aaagactttg tgccggttta aaatacgcta tgttcagggc tgccgaggaa
     2581 ggccatgttt gccttcctgt cgacgagtta ctatatagag cctcgcagct tttagatgta
     2641 gaaatcgagc ttttaaagca ggccttgctg gcccttaaag agaaaaaaga tatcatcacg
     2701 gattgctctt ggggcagcga agatgtatat ttgacttcct ttcaccgctg tgaaagtgcc
     2761 gttgcccgca ggcttttttt attggccgcc atggtagata aaactttgga tattagtgat
     2821 gaggatatag acaagataga aaaacgctgt aatataaagc tggcagcccg acagcgagaa
     2881 gcactggaaa aggctgcaca gcacggtgtg gtagttataa cggggggacc tggaacggga
     2941 aaaacaacta caatacggag ccttttggaa ttcttcaaag gccatgattt gaaggtggcc
     3001 ttggcggctc caacagggcg tgcagccaag cgaatgatgg aggccacagg catggaggcc
     3061 aagaccattc acaggttact ggagtataag gcgtacgaag aaggcggtat ggcttttggc
     3121 aaagaccaca acaatccact cgatgaagat gtaatcatca tagatgaagt gtcgatggtc
     3181 gacattatac tgatgcagca cctgctatca gcggtgagac ttggagcaag gctgatattg
     3241 gtgggagata aggatcagct gccttctgtt ggaccgggca gtgttttaaa agagataata
     3301 gaagccgaga gactaccggt agtggtcctg gatgagattt tcaggcaggc acaggagagc
     3361 atgattgtag taaacgccca taggattaac aaaggctatt ttccctacct taacgtaaag
     3421 gacaaggact ttttctttga acaggtcctt ttgcccgagg atatactgca gacaatttta
     3481 gagctggtga caacacggct gcctaaatac aaaaatttcg atcccatgga agatattcag
     3541 gtacttacgc caatgaaaaa gggcttactt ggtgttcaca atctaaataa cagcttacaa
     3601 gggctgctta atccccccaa acccggcaaa agagaagtgc actttcgctc tcaaattttt
     3661 cgggaagggg acaaggttat gcagattaag aacaactatg agaaggaggt tttcaacggc
     3721 gatttgggcc gcatagtcaa aatcgatgaa gagcaagttt tggtgacatt tgctgattcc
     3781 tggcaggagc gggatatagt ttaccagctc caggaattgg aggaactaaa cctttcttat
     3841 gctttatcgg tacataaaag tcagggcagt gaattccccg tagtaataat gccgatatcg
     3901 acccagcact atgttatgct gcagcgcaac ctcctgtaca ccgctgttac gcgggcgaaa
     3961 aaattactgg tccttgtagg cacaaaacaa gccctgacaa tagctgtgca aaacaataaa
     4021 tccatcaggc gttacgggca tttgagtgac aggatcatct gtgaattttc tcaggcgaaa
     4081 agtgtttaat gatattggtg gattttttgt caagtgcttt ttttactctt ttttttattt
     4141 aatgtggatt cttattagcg agtaaggggt gaagctgtat gttggatgaa aaactctcta
     4201 agctgaagga aagcattaag gcccttggaa aggtggtagt agcattttca ggcggagttg
     4261 acagcacttt tttgctaaag acctgtattg atgaactggg gcgcgacaat gttttggccg
     4321 tgacagccca ttctcaggcc attaccgaac gtgaacttat agaagcacag gccttggcag
     4381 ctctcttaaa tgccaggcaa gtaatcgtag agtccaccga actggatgac cccaatatta
     4441 cagataatcc acctgaaagg tgctactatt gcaagaagca aatttttagc aattttactg
     4501 ccattgccag ggagaatggc tacaatttta tattagaagg cacaaatttc agcgatagtg
     4561 atgatttcag gcccggcatg gcggccataa aggaactcgg tgttttaagc cccttaaaag
     4621 atgccggcct gacaaaagat gaaatacggg agctttctaa agcaatgggc cttcccacat
     4681 ggaataaacc ttcatatgcc tgcctttttt ccaggttccc ctatggagaa aaaattacgc
     4741 cggataagct aaaaagggtg gccaaagctg aagacatttt aaggcaatat ggtttttgcc
     4801 aatttcgggt gcgaagccac ggcgacctgg ccaggataga gttcttgccc caggaaatgg
     4861 acaaagtttt cgacgagtct tttcgaaata cagtgataag tgaatttaag aagataggct
     4921 ttatctatat taccctggat ttagcaggct ataggtccgg aagcatgaac gaaggactga
     4981 aagaggagga tatggcactt tggaaaaatt gagacatttg cttgaaaagc tgaaggccgg
     5041 ggaggtagcg gttgatgagg tattagaagc tctaaaaaac ctgccctatg aaaatctagg
     5101 ctttgcctgt attgacaacc atcgcagtct cagaaggggt gtgccagaag taatatactg
     5161 tgccggcaag acgccgtccc aggtggcaga aataacaagg cacatgctca gaaacggcag
     5221 taatgtgatg ggaacccgtg ccgacatggc cacatttgac aaggtacggg aggtcgctga
     5281 agatgctaag tactacgatg ttgcgaggat ttttgcggtg cagcgggagg agcagccaaa
     5341 gaacaaaggc aagattgcag tggtaactgc gggcacttcg gacatacccg tggccgaaga
     5401 ggctgccgtg actgcggagt tgttcggaaa tcctgtggaa agactttacg atgtgggagt
     5461 tgccggtatt caccgcttgc tgatgaacat ggaagtcctt cgccaggcaa gagtcataat
     5521 tgtcgttgcc ggtatggaag gagcactcgc cagcgtagtc ggaggcttgg tagacaaacc
     5581 agtgattgca gttcccacta gtgtgggtta cggtgcacac tttaacggtt tggcagcttt
     5641 gctcgctatg ctaaacagct gtgccagcgg catatctgtt gtaaacatcg acaacggctt
     5701 tggcgcagcc tatgcggcac atcagataaa ccggctgtgc gaaaacaata attaatgtta
     5761 aaaatgaggg agtgagtacc ataaaaagtt cgactgaaaa aaacatctta taccttgatt
     5821 gtttttcggg cataagtggt gacatgtttc taggtgccat gctcgacttg ggcctggatg
     5881 aaaaagagtt tttagctgaa cttaaaaaac tacccctaaa ggattatgaa gtggaaatca
     5941 aaaaatcaat aaagaagggc attaccggaa cggatgtcac ggtaataacc aatgaacatc
     6001 atccccatcg aggacttaag gaaatatatg aaatcatcga caatagcagc ttaaactcca
     6061 acgtaaaaga aaagagtaaa gaagcttttt acaaattggc ttcagtggaa ggtaaaattc
     6121 acggcaaatc tccctatgaa atccattttc atgaagtggg tgcggtggat tcaatagtag
     6181 atatagttgg cgcatgcatc ctaatggata tgctaggccc aagcagagtt tgtgcatctg
     6241 ccatcaacgt aggctttggg actgttaagt gcgctcacgg tattttgccg gtaccggcgc
     6301 ctgccactat ggagctttta aaaggaatac cggtctactc aggagaggag cccggggagt
     6361 ttacgacacc aacaggagcc cttatattaa gtacattcgt agaaaaattt ggtacaatac
     6421 cccccggaat tccagaaaaa tccggttacg gtttaggcaa agcagaacac aagtccccca
     6481 atgttttaag ggcagtattg attaaatcat tggataatag tgctgatata cacagccggg
     6541 gccttgaaga ggaccaggtc gcatttttgg aagcaaatgt ggacgacatg aatccggagc
     6601 tttacgagca tgtaatagag agcctttttc aaaaaggggc cttggatgta tttctcacac
     6661 cggtcataat gaaaaagagc aggcccggaa taaaaataag ttgcatctgc acgcaggata
     6721 aaaaactgct tttatctgaa actattctga gagaaaccac tacgctgggt gtcagggagt
     6781 tggaagttgc ccgcctaaag ctatccagga caattgaaaa agcgagcacc ccttttggtg
     6841 atataaggat caaagtatcc aagatcggtg atgagattgt cagagttacg cctgagtacg
     6901 aggacatgaa aaaattggcc aaaaagaaca atatgccctt gttgaaggcc tatgaagaaa
     6961 taaagccttt ctgcaagaag tagtagtttt tcttgtaaag ctgattcctt agtgttatac
     7021 taatttcagg ttttattgac aatagctttc acaaggctaa atttctatgt gagtcttaac
     7081 taatttaata acatgggagt gttttatcga tgccagacaa agttagagta agatttgcac
     7141 ctagtcccac gggcagcctt cacataggtg gtgcgcgaac ggctcttttc aacctgctct
     7201 ttgcccgtca caacaacgga acttttgttt tgagaataga ggataccgac accgagcggt
     7261 caactgaaga atccgcagcc cagataatac gctcaatgag atggttagat cttgattggg
     7321 atgaaggacc agaaaaaggc ggagattttg gtccttactt tcaatctcaa agacttgaac
     7381 tctacaaaaa agaagtagat aggcttttag ctgaaggaaa ggcatatcgc tgctactgca
     7441 ctccagaaga gcttgccgag cgacgggaag cggctttaaa agcaggcaag cctccgaaat
     7501 atgatggcac ctgccgaaat cttactgccg aacaaattga gaaatatgaa gcggaaggca
     7561 ggaaatatgc cataaggctt aaaatgcctg aagaaggtca aacagaggtc aacgacttaa
     7621 tccgaggcag ggtagtcttt gaaaactcgg ttttagatga ttttataatt gtaaaatcaa
     7681 acggaattcc cacttacaat tttgcgtgcg tcattgatga caatgccatg caaatcaccc
     7741 atattatccg tgccgaagaa catctttcaa atacacccaa gcagatacag gcatatttag
     7801 ccttaggata cgaaatacca cagtttgccc acgtgccgat gattcttgca ccggatcgca
     7861 gcaagctcag caagcgccac ggtgcaacat ctgtggaaga atttagagag cagggatatc
     7921 tggcaaaggc cataatcaat tacctcacct tactgggttg gtcacctgag gggacggaag
     7981 aaatctttga aattgacaag gccataaagg aatttactct ggagcgggta aacaagaccg
     8041 cggcaatata tgatgtcaag aagctgactt ggatcaatgg ccactacatg cgtgaactgg
     8101 actctgcttt tgtcacggaa gagacaatac cgtttatgat aaaaaaagga ataattaccg
     8161 agcaggaagc caaggaaaaa tttgactata tcaggcgaat tgttgaaatt tcaagggaca
     8221 gggccaaaac tttagacgag ttggcagatg ccatagcttt tttctttaaa gacgtgtcgg
     8281 aatatgagga gaaaggcgta agaaaacatt tcacaaaaga aaatgccgcg aagcttttaa
     8341 ccttgggggc agatgcactg gaaagattag atgactttac acttgaaaag accgaggcaa
     8401 cctttagaag catcacagaa gagttgggac tcaaagctgc ggaaataatt catcctacgc
     8461 gccttgctat caccggcagg acgataggcc ccggcctttt tgacattatt gtactgctgg
     8521 gcagggaaaa aaccgtagaa cgcatgagaa gagctgttaa atggatcagt gaaaatgtgg
     8581 agtcctagga atagaatgat aaagggatgt tgaaatgttt tcgactttca gcattccttt
     8641 tttttatttg tagcaggatt tttgataagt tttgcataat catatagaat aaaaaataga
     8701 gttttcatat gatctgggag ggtgtttagt gtttggagaa cttgtaggag tagtaatatc
     8761 catttttttt gtggcaattg tcctcgtatt gtcggaatta ataaggagac tgggaaagtt
     8821 tagcacggaa ttcactcgca agtttgtaca tattggcgtt tctcattggt ggctagtagc
     8881 catgttttta ataggggata tccgctatgc cctaattccg ccagttattt tcgtttttct
     8941 gaactactat tcttataaaa aagaactgat taagagcatg gaaaggggta aagacagttc
     9001 cgacctaggt accgtttatt tcccaatatc actcatcgta ataattatgc tcacatggaa
     9061 cgggggtttt ctgggcgaag atttaaaaca cctgggagct cttggcgttt taaccatggg
     9121 ctacggtgac ggttttgcgg cgataatcgg agaaaatttt gggcgaagga aatacgaaat
     9181 ttttggcaac cgaaaaagcc tggaaggctc catagcgatg tttggctttg cctttctagt
     9241 ctctgccatt atacttggaa ataatattag ttttgaattc gatgttttca ggataagttt
     9301 tgtgatcgca gttttagcag ctataattga ggctatcaca ccctatggct ttgacaatct
     9361 tacggttccc atatttacta ctttaaatgc ctactatttt gtaaaaggaa gtccagcttt
     9421 tttgttcata tatatggcct gtataggctt tgtgatcagt ttttttattg cctatgccgc
     9481 ttacaagaaa gaatccctga ctcttgacgg ttctataggt gccacctttt tgggcacact
     9541 catgtatgcc acatcaggcc ttttcggctc aatcatgatg gtagtgtttt tcctatcttc
     9601 cagcatccta agccatttta agaagaataa aaaaggtaag gctgcccggc agtttgataa
     9661 aacaggccga cgggttatta tgcaggtatt cgcaaatggc ggtgtggggc ttatccactc
     9721 catcttgtat tacattacaa aaaatcctgc ttatttagta tttctaggcg tttcttttgc
     9781 tgccgccaat gcagatacct gggccacgga attgggtttg ctaaacaaga aaaaccctat
     9841 ttcccttaga accttcaagc ctgtggaaaa aggcacttcg ggagccataa gtctgtttgg
     9901 caccttttcg gctttgattg gctctatgct gattgggata tttgcaactt tcggtttttc
     9961 cttattaaac ataaacatag ctgaactagg ccttgaagtt acccatgctt ttcaaatggt
    10021 aactttaggc ggctttatag gctccttaat tgattcaata ttaggggcta cggtccaggg
    10081 cgtttattat tcccatgaac ttaaaggaga aacggagaaa aaagagtata acggcaggcc
    10141 caatttgctc ataaggggct tcggctttgt aaacaacgat ctggtcaact ttttgagtat
    10201 aggaatctca agcatattgt tcttgggaat cgcttagcta tctagtatgc agctcaaaaa
    10261 agatttagat tgtgtttcca gtaaaaagtt gctgaaaaaa gtggacaagt atggtattgt
    10321 aaaataaagc gacatattgt cataatttta ccggcaagga gaggttttgg tgaatttttt
    10381 cagattagct ttgtttggtg tgtttttgct tttggtggtc ttgctggtgc ccttagttgc
    10441 cggagagcaa gggcaactga tacatgcagt agagggagct aaggaagtgg ttaatggtga
    10501 agttgtcgag gagccgagtc ctgtagatga accggcaaat aatgaagctg tcgaggaaaa
    10561 gccttatccc ataccgcctg aaaagcagga ggaagaagcc ttgtttccac ggaacatgac
    10621 cgttttgaat gcaagtgaaa taacagcagt gaacatcagg gaacgaccga cagccgattc
    10681 aaagagcatc ggcatagtat acgggaacct catgcatgta gatgtcatac aaaatctaga
    10741 caacggctat tcggaaatct ccacgtggga ctacgcaagc atgaaagata tagtgggctt
    10801 tgtccccaca gagctcctga agacggttga gctgaat
//
LOCUS       DAONUO010000023        10704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4763_length_10704_cov_3.935769, whole genome shotgun sequence.
ACCESSION   DAONUO010000023 DAONUO010000000
VERSION     DAONUO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10704
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4763_length_10704_cov_3.935769"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..150
                     /locus_tag="PK768_01525"
     CDS             10..150
                     /locus_tag="PK768_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HQA59680.1"
                     /translation="MTFGGGFPIFCGDEVIGAIGVSGGSVEEDEMVARAGLKIITEGS
                     EF"
     gene            153..1538
                     /locus_tag="PK768_01530"
     CDS             153..1538
                     /locus_tag="PK768_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HQA59681.1"
                     /translation="MIQEELISIIKDKILRELSKDTSSDESELGIFDTVDEAVAAAKE
                     AQKKLNLLDLEKREIIISAIREASINNANYLAALCVEESQMGRVDDKIAKIILAAKKT
                     PGTEDLIPWAKSGDHGLTLVERGPFGVIASITPVTNPVSTIVNNGIGMIAGGNTVVIN
                     PHPRAIRVSIETIKLINDAIAKAGGPRHLICTVKEPTIETSQQMMKHPDVRLISATGG
                     PGVVKAALSSGKKAIGAGAGNPPVLVDETADIAKAAHDIVLGASFDNNLPCISEKVAI
                     VVEEVAQKLMDAFKNENVYILNDDEARAITEKVIVEGAVNKELVGKDASYILRQIKVE
                     PISDVRLLIMPADKDHPLVHHEQLMPVLPVVTVSDVDEAIDFAVKCEQGNRHTAIMHS
                     KNVDHMTKYARAIETTIFVKNGPSYAGIGMGGEGFTTFTIAGPTGEGLTSARSFTRQR
                     RCILVDGFRIV"
     gene            1566..2900
                     /locus_tag="PK768_01535"
     CDS             1566..2900
                     /locus_tag="PK768_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQA59682.1"
                     /translation="MEIRETLREKGVVGAGGAGFPTYAKLKQGGIDYYIANGAECEPL
                     LDVSKEIMARFPDRVVKALNILKDSTGAKRAVIALKGKYKQAIKALDEELSQTGSDIE
                     LFQLGDFFPAGDEQVMVYEVTGKVVPEGGIPIAVGAVVNNIETLFNVYGAIEEDAPVT
                     QKFITVGGQVKDPITLKVPVGTTIGWLLDFLGVDYKDKVIIDGGPMMGNIADPSAPIT
                     KVTGGLLIFPKGHPAVTVKESDISRILKLARIACIQCRYCTLQCPRYLLGHDLEPSKI
                     MLSVGFKLTDGYIKQAMLCCECGVCEAYSCPQQLSPRRVNITLKRELAKTGFRYTTDK
                     TNFKARDERQWRKIPTSRLKLRLQLMEFDRPAHLVDGEFVKPPEVHIPMKQHIGAPAV
                     PVVGVGEKVTIGQVIGRVPEDQLGCNVHASIDGTVAHCDEQKIVIRGEDAKW"
     gene            2894..3451
                     /locus_tag="PK768_01540"
     CDS             2894..3451
                     /locus_tag="PK768_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HQA59683.1"
                     /translation="MVRAIGLVETISIAAGIETADAMIKIAPVKLFIARPICPGKFLI
                     LVGGEVGAVKNSLKEGCRKADEYLADSVLIPRVHEDVFPALEGGIVLDDLDSLNSLGV
                     IETFSVTASILAADAAVKAGNIKLVEIRPAIGLGGKSYVTMLGNVSAVKTAVEAGCQK
                     AEPEGTLVKSVVIPNASKDLLISLL"
     gene            3739..4518
                     /locus_tag="PK768_01545"
     CDS             3739..4518
                     /locus_tag="PK768_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQA59684.1"
                     /translation="MPRVPRQFSKTGFYHIMMRGLNRERIFEKVDAKKTMLNFLAEKV
                     ADSDVGIYAYCIMDNHIHLLLKAEPEDLVAYMKKVNGSFASHYNREENRIGPVFQGRY
                     KSECVEDERYFWGVLRYIHRNPVQAYMVKVPQEYEWSSIKDYLSGKSRLLDEKALQLK
                     RENFSDNESFLKMHEEEDLKIYLDVNEDLDKMKGSVARKLIQRALQENGASHVLELRQ
                     KGNAFQNLLDKLAVEVKLTYKEIAELTDLSYSMVQRLVSKA"
     gene            4593..5849
                     /locus_tag="PK768_01550"
     CDS             4593..5849
                     /locus_tag="PK768_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HQA59685.1"
                     /translation="MNKKTIIKAAMIALIFVIFMSQGALASEKAIAVIANGERLTFDV
                     PPILHNNRTLIPFRAIFEAYGAQDIAWNQELQKATGSVDSTTIELTVGSNIALVNGSH
                     VELDVPPIVKNGRVLVPVRFISEALGYDVEWDGAKREVIIKSPDYYNAFYNDALGVKL
                     RIGQSVDELTAIMGKPSRIDKSGKYYDWYIYNDEIEHYMQVGVYENKIVAFATNASSW
                     VLNNDLRIGDFKEVIEKKYELRGSFIYLKEDSLEILWDIHDNYSLSTFILESTNAPNS
                     NYSFLSFGEDYFSEEVARSFERQAFDLTNVYRLRHGRQPLAWRKDLAEMALYHSKDMA
                     ANGYFSHDSLNGDTLADRAKKFKVSYRSLGENIAKGYGNTLRAVGGLYNSKGHRENML
                     TSEFKYLGVGVAFSGSTLYLTQNFCY"
     gene            complement(5895..8471)
                     /gene="clpB"
                     /locus_tag="PK768_01555"
     CDS             complement(5895..8471)
                     /gene="clpB"
                     /locus_tag="PK768_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HQA59686.1"
                     /translation="MDISKYTQKSQEAILKAQDLAVEHRHQEVTSRHLMYALLRQEDG
                     IIPRLMQKIGCDLNLLKSLAEDAVNRIPSVHGYDGPLAMNTGLARVFLRAEKEAQNMK
                     DMFVSVEHLLLALLEEGDHEVKQVFKKAGISRNSVLTALKDLRGSQQVTGENPESTYE
                     ALERYGRDLTRLAREGKLDPVIGRDDEIRRVIEILSRRTKNNPVIIGEAGVGKTAIVE
                     GLARRIVAKDVPDGLKDKRIIALDMGSLIAGAKFRGQFEERLKAVLKEIQESDGQVIL
                     FIDELHTVVGAGAAEGALDAGNLLKPMLARGELRAIGATTIDEYRKYIEKDAALERRF
                     QPVMVNPPSVEDTISILRGLKEKYEVYHGVRIQDNAIIAAATLSDRYISDRFLPDKAI
                     DLMDEAAARLRTEIDSMPVELDDIKRKIIQLQIEETALKKETDDLSLKRLESIRQQLA
                     ELNKEFEELKTIWEREKEEISRIHKIKSEIEAVKLEIEKAEREYDLNKAAELKFGKLN
                     QLERELRVQEEAIKQRQNKKTLLKEEVDEEEIARVVSQWTGIPLNKLMEGEKQKLLQL
                     DEILHRRVIGQDEAVKAVADAVLRARAGIKDPGRPIGSFIFLGPTGVGKTELARALAE
                     ALFDDERNMIRIDMSEYMEKHSVARLIGAPPGYVGYDEGGQLTEAVRRKPYSVILFDE
                     IEKAHNDIFNVLLQILDDGRLTDGKGRTVDFKNTVLIMTSNIGSQEILDFQAGKGEDF
                     SKIKEKVLDLLKYHFKPEFLNRVDEIIVFEPLGPHHMEKISELLLKRFAQRIMDSIGL
                     ELTWSEKTIKYLAEKGYDPTFGARPVKRLIQQSIETPLSRRVIQGIKPGEKINLDEFI
                     AE"
     gene            8956..10704
                     /locus_tag="PK768_01560"
     CDS             8956..10704
                     /locus_tag="PK768_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQA59687.1"
                     /translation="MNINFTELSKGYKGKMVFKKISGQITDGDRVGLVGANGVGKTTL
                     AKLLVGLEEKDSGTITYSPPHMKLLYVEQYPVFDKDVTVYEEAIRIALKFNGNRANVA
                     AIAKKGLSKVGLKEDKWKQKAMSLSGGEKTKLALYKALAADFDLLILDEPTNHLDAKS
                     LEWLEEFVLSIEKPILVISHDRFFLDKVANKIWELTSQELKAYEGNYSSYKEQKEIEI
                     KSLTREYNKEQMRISQLKEEINDRRNWYNSAHKGAGTNDFYRSRATKHARALKAKQRQ
                     LERIEKEKIEKPKKPVSPAFDIINKNIIGRKFPRFLVQGKNLAKKYGNKQIFQDVTFN
                     IQHQDKIALLGENGVGKTTLLKIICGLDKDFCGNLKINPSVKIGYFAQELDNLNSEAT
                     VLDDVLLEGNTVEETRLLLAGLLFRGDDVFKKIRNLSMGEKGRVAFAKLILSGANLLV
                     LDEATNYMDIVSKEKIEEVLEELRGSIIFVSHDRYFIRRLANKVFTIENRKLYCFEGN
                     YDYYLAKRKDQQAQKEIGTDYRTVAENIKRLELELAFLSGKLDETVDEEEKEKLNERF
                     LAVARELNKNRELLKL"
BASE COUNT         3046 a         2153 c         2645 g         2860 t
ORIGIN      
        1 ggaaggtacg tgactttcgg aggaggtttt ccgattttct gcggcgatga agtcataggt
       61 gctatcggag tcagcggggg gtctgttgaa gaagatgaaa tggttgcaag ggcgggcttg
      121 aaaataataa ccgaagggag tgaattttaa ttttgattca ggaagaacta atttctatca
      181 taaaagataa aatcttaagg gagctgtcga aggatacgag ctcagatgaa tcggagctgg
      241 ggatattcga taccgtagat gaagcggttg ccgctgcaaa agaggctcaa aagaagttaa
      301 acttgctgga cctggagaaa cgggaaatca tcatatccgc tattagagaa gcaagcatta
      361 acaatgcaaa ctacctggcg gccctttgtg tggaagaatc ccagatggga agggtagatg
      421 ataaaatagc aaaaattatc ctggcagcga agaaaactcc cggcacggag gatttaattc
      481 cctgggcaaa atcaggcgac catggtctca cccttgtgga aagaggtccc tttggtgtga
      541 tagcttcaat aacgcccgtt acaaatccgg tgtcaaccat cgtaaataac ggcataggca
      601 tgattgcagg aggcaacaca gtagtaataa atccccatcc ccgggccatc agggtaagca
      661 tcgagacgat aaagctcatc aacgatgcca tcgctaaagc cggtggcccc agacacctga
      721 tttgcaccgt taaggagccg accatagaga caagtcaaca gatgatgaag catcctgatg
      781 tcagattgat ttcggccacg ggaggcccgg gtgtagtaaa agctgcactc tcctcaggca
      841 aaaaagccat aggggctgga gcgggcaatc ctccggtgtt ggtggatgag actgcagata
      901 ttgccaaagc tgcccatgat atagtgctgg gggccagttt tgacaacaac ctcccctgca
      961 tctcagaaaa ggtggccatc gtagttgaag aggtggccca aaagctaatg gacgccttta
     1021 aaaacgagaa tgtatatata ttaaacgatg atgaagcaag ggcaattacc gaaaaagtta
     1081 tagtagaggg tgctgtcaac aaggaactgg taggcaaaga cgcaagctac attctgaggc
     1141 agattaaggt ggagccaatt tccgatgtgc gattgctgat aatgcctgca gataaagacc
     1201 atcccctggt ccatcatgag caactgatgc cggttttgcc ggtggtgacg gtttccgatg
     1261 tggatgaagc cattgatttt gcagtgaaat gcgaacaggg caaccgccat acggcaatta
     1321 tgcactcgaa aaatgtggac cacatgacaa aatacgccag ggccattgaa accactattt
     1381 ttgtgaaaaa cggcccttca tatgcaggca tcggcatggg aggagaaggc tttacaacct
     1441 ttacgatagc gggccccacc ggtgaaggct tgacttctgc caggtcattt acccgtcagc
     1501 ggcgctgtat attggtagac ggatttagga tcgtttagca gatttggcag ctaggagtgg
     1561 taaacttgga aataagggaa acattgaggg aaaaaggtgt agttggagct ggaggcgcag
     1621 gatttcctac ctatgccaag ctcaaacagg gaggcatcga ttactacata gcaaatggcg
     1681 cggaatgcga acccttactg gacgtcagca aggagatcat ggccaggttc cccgatagag
     1741 tcgtaaaagc cctgaatatt ttaaaagatt ctacgggtgc caaaagggct gtaattgcct
     1801 taaaggggaa gtataagcag gccataaagg ccctggatga agaactgtca cagacgggaa
     1861 gtgacattga gctttttcaa ttgggagatt tcttcccggc aggagatgaa caggtgatgg
     1921 tctatgaagt caccggtaag gtggtgcctg aagggggaat ccccattgca gtgggagcgg
     1981 tggtaaacaa cattgaaaca ctgttcaatg tctacggggc cattgaggaa gacgctcccg
     2041 tgacccagaa attcatcacc gtgggaggtc aagtgaaaga tcccattacc ctcaaggtgc
     2101 cggttggcac gacaatcggc tggctcctgg attttctagg tgttgattat aaagacaagg
     2161 tcatcatcga cggcgggcca atgatgggaa atattgcaga cccaagtgca cccataacca
     2221 aggtgacggg cggcttgctg attttcccca aagggcatcc ggcagtaacc gtaaaggagt
     2281 cggacataag ccgcattttg aaactggcca ggattgcctg tattcagtgc aggtactgca
     2341 ccctccagtg tccccgctat ctcctgggcc atgatttgga gcccagcaaa ataatgcttt
     2401 ccgttggctt taagctgaca gatggctaca tcaagcaggc gatgctatgc tgtgagtgcg
     2461 gagtgtgtga agcttattcc tgcccgcagc agctatcccc aaggcgggtc aatattactt
     2521 taaaaagaga attggccaag acaggtttta gatatacgac tgacaagact aactttaaag
     2581 ctcgggatga aagacagtgg cgcaaaattc ccaccagccg tttaaaactc cggctgcagc
     2641 ttatggaatt tgacaggcca gcccatttag ttgacgggga atttgtaaaa ccgccggaag
     2701 ttcacattcc catgaagcag catatcggtg ccccggcagt tccggtggtg ggagtgggcg
     2761 aaaaagttac aataggtcag gtaatagggc gggtgcctga agaccaattg ggctgtaatg
     2821 ttcatgcaag tatcgatggg acggtagcgc attgtgatga acaaaaaatt gtcatccggg
     2881 gggaggatgc caaatggtaa gagcaatagg acttgtagag accatcagca tagctgcagg
     2941 tattgagaca gctgatgcaa tgataaaaat tgcaccggta aaacttttta tagccaggcc
     3001 tatttgcccc ggtaaattcc taatccttgt tggcggcgag gtgggagcgg tcaaaaactc
     3061 cctgaaagaa ggctgccgaa aagccgatga atacctggcc gattccgtgt tgattccccg
     3121 ggttcatgaa gatgtatttc ctgccctgga aggtggcatc gtactggatg atcttgactc
     3181 cctcaactct ctaggagtca tagagacctt ctctgtgact gccagcatcc ttgctgccga
     3241 tgctgccgta aaagcaggaa atataaaact tgtggagata aggccagcca ttggccttgg
     3301 cggcaaatcc tatgtgacca tgctaggcaa tgtaagtgcc gtaaaaacag ccgtggaagc
     3361 cggttgccaa aaagccgaac ccgaaggaac cctggtcaaa agcgtagtca tccccaacgc
     3421 atccaaagac ctactaatct ccctcctctg aatatttcgg gacgggtaca aaaaactcat
     3481 tcctcctttt gaatatttcg ggacaggtac aaaatattca tacccctttt aaaaaaaagc
     3541 atccatgaga tgctttttta ttttgtagat aagtaatcat agacatttta agaaaaatca
     3601 gttatgttat tgataacgaa tattttggga caggtacaaa atattcacaa gcggtttcaa
     3661 tatagatgaa taatatgtat ccgtcccgag atattcagat attcatttaa atattcacta
     3721 ctgtaagggg tgattgccgt gccgagagtg ccgcgacaat ttagtaagac ggggttttac
     3781 cacattatga tgcgtggcct aaatcgtgag cggatttttg aaaaggttga tgcaaagaaa
     3841 acaatgctga attttttagc tgaaaaagtt gcggacagtg atgtaggcat atatgcctat
     3901 tgtattatgg acaatcatat tcatcttttg ctcaaggcag agcctgaaga tttggtggct
     3961 tatatgaaaa aagttaatgg ttcttttgca tcgcactaca atcgggaaga aaatagaata
     4021 gggccggtgt ttcaggggag atacaagagt gagtgcgtag aggatgaaag gtatttctgg
     4081 ggtgtcttaa gatacattca tcgaaatccg gtgcaggcat acatggtaaa ggtacctcaa
     4141 gagtatgaat ggagcagcat aaaggactat ttatcgggca agtccaggtt gcttgatgaa
     4201 aaagcgctgc agctaaagcg ggaaaatttt agcgataacg agtcatttct taaaatgcat
     4261 gaagaagagg atttaaaaat ttaccttgac gtaaatgaag acctggataa gatgaagggg
     4321 tccgtggcca ggaagctgat tcaaagagca cttcaagaaa acggagccag ccatgtcctt
     4381 gagttgagac aaaaaggcaa tgcctttcaa aacttattgg acaagctggc cgtggaggta
     4441 aagttgacat ataaggagat agcagaattg acggatttaa gttattctat ggtgcaacgg
     4501 ctggtgtcaa aagcctaatc gatgtaatcg atgtgtatga cgatgaataa tttgtacccg
     4561 tcccaaaata ttcatttaaa taggaggtcg ctatgaataa gaagacaata attaaagctg
     4621 ctatgattgc attaatattt gtcattttta tgtcgcaagg agcacttgcc agtgaaaaag
     4681 caattgctgt aattgcaaat ggtgaacgat tgacatttga tgtcccgcca attttacaca
     4741 ataacagaac tttgataccc tttagggcga tttttgaagc ttatggggca caggacatag
     4801 catggaacca ggagttgcaa aaagctacag ggagcgttga cagcactacc atagaactga
     4861 cggtaggctc aaatatagcc cttgtcaatg gaagtcatgt ggagctggat gttccgccga
     4921 ttgtgaaaaa cggaagagtg ttggtgccgg ttcgattcat atccgaggcc ttaggctatg
     4981 atgtagaatg ggatggtgcc aaaagagaag ttattatcaa gtctccggat tattacaatg
     5041 cattttacaa tgatgcctta ggcgtaaaac ttagaattgg ccaaagtgtt gatgaattaa
     5101 cagcgatcat gggaaagccc agcaggatcg ataagagcgg caagtattat gattggtaca
     5161 tatacaacga tgaaatagag cattacatgc aggttggggt ttatgagaat aaaatagttg
     5221 cttttgccac gaacgcatcc tcctgggtgt tgaataatga tttgagaata ggtgatttta
     5281 aagaggtcat agagaaaaag tatgaactac gaggaagttt tatatatttg aaagaagaca
     5341 gtcttgaaat attgtgggac atacatgaca attatagctt gtctacattt attttagaaa
     5401 gcacaaacgc tcctaacagt aactatagct tccttagctt tggcgaagat tattttagtg
     5461 aggaagttgc caggtctttt gagagacagg cctttgattt gacaaatgtg tataggctta
     5521 gacacggtag gcagccttta gcctggcgaa aagacctggc tgaaatggca ttgtaccatt
     5581 ccaaggatat ggctgcaaac ggttacttca gccatgattc attaaacgga gacaccttgg
     5641 cagacagggc gaaaaagttt aaggtaagct accggagttt aggagaaaat attgccaagg
     5701 gttatggtaa tactttaagg gctgttggag gtttgtataa ttcgaaaggc catagggaaa
     5761 atatgctaac aagtgaattt aaatatttgg gagtcggtgt tgcttttagc ggaagcacat
     5821 tatacttaac acaaaacttt tgttactaat gcataataag cccagatgaa taatttgtac
     5881 ccgtcccaaa atattcattc agctataaat tcatctagat ttatcttttc gccgggtttt
     5941 attccctgta taacgcgcct gctcaatggg gtttcgatgc tttgctgtat aaggcgcttt
     6001 acaggtcttg cgccgaaagt tgggtcatag cccttttcgg ccaggtactt tatggtcttt
     6061 tccgaccagg taagttcaag gcctatagag tccattatcc gctgggcaaa gcgttttaat
     6121 aagagttcgg agattttttc catgtggtgg gggccaaggg gctcaaatac aattatttca
     6181 tctactcggt ttaaaaactc gggcttgaag tggtatttca agagatcaag tactttttcc
     6241 ttaattttag agaaatcttc tccttttcca gcctggaagt ccaggatttc ctggctgccg
     6301 atgttggagg tcattatgag gaccgtgttt ttaaaatcca cggtgcggcc cttgccgtcg
     6361 gtgagcctcc cgtcatccag tatttgaagg agcacgttaa atatgtcgtt gtgggccttt
     6421 tctatttcat caaagagtat taccgaataa ggcttcctgc ggacggcttc ggtaagctgg
     6481 ccgccctcat cgtaacctac atatccgggc ggtgctccga tgagccttgc cacagagtgc
     6541 ttctccatgt attccgacat gtctattcgg atcatatttc gctcatcgtc aaaaagagct
     6601 tcggctaagg ccctggccag ttcggttttc cccaccccgg taggtcctaa aaatatgaag
     6661 ctccctatgg gccttccggg gtctttgatg cccgccctgg cccggaggac cgcatcggct
     6721 actgccttta ccgcttcatc ctggccaatg accctgcggt gaaggatttc atcaagctgc
     6781 agaagttttt gcttttcgcc ttccataagc ttgtttaagg gaatgccggt ccactggctg
     6841 acgacccgcg ctatttcttc ttcgtcaact tcttccttca agagggtctt cttgttttgc
     6901 ctttgtttaa tggcttcttc ctgaaccctt aattctctct caagctgatt tagttttcca
     6961 aatttgagtt ccgcagcttt attcaggtca tattcccgct cggccttttc aatttccagt
     7021 ttgacggcct ctatctccga tttgatttta tgaatacggc ttatttcttc tttttccctt
     7081 tcccatatgg ttttcagctc ttcgaattct ttattgagct cagctagctg ctgtcgaata
     7141 ctttccaacc tttttagcga caaatcatcc gtttcctttt ttaaggcggt ttcttctatt
     7201 tggagctgga ttatcttgcg ctttatgtca tcaagttcca caggcatgct gtcaatctcc
     7261 gtgcgcaatc tagccgctgc ctcgtccatc aggtcaattg ccttgtcggg cagaaacctg
     7321 tctgaaatgt acctgtccga aagggtagct gccgctataa tggcattgtc ctggattcgg
     7381 acgccgtgat atacttcata cttttccttg agacccctca gtatggaaat ggtgtcttcc
     7441 accgatggcg gatttaccat taccggctgg aagcgccgct ctagggcagc gtctttttca
     7501 atgtattttc ggtactcgtc tatggtggtt gcacctatgg cccgcagctc cccacgtgcc
     7561 agcatgggct taaggaggtt tccggcatcc agggcccctt cggcggctcc ggcaccgact
     7621 actgtgtgga gttcatcgat gaaaagaatg acctggccgt cggactcttg tatttctttt
     7681 aagacggctt tcagccgctc ttcaaactgg ccacggaact tggccccggc gattagggaa
     7741 cccatgtcaa gggctatgat gcgcttgtct tttaggccat cggggacatc ctttgccact
     7801 atgcgccggg ccaggccttc cacaatggca gtcttgccca caccagcttc gcctatgatg
     7861 acggggttgt ttttggtgcg ccgtgatagg atttcgatga cccgccttat ttcatcatcg
     7921 cggccgataa ccgggtcaag tttgccctcc cgggccaggc gggtgaggtc cctgccgtac
     7981 ctttcaaggg cttcatacgt agattcaggg ttttcaccgg ttacctgctg gctgcctcga
     8041 aggtctttta gggcagtaag gacgctgttc ctactgatac ctgccttttt aaagacctgc
     8101 tttacctcat ggtcgccttc ctcaagcagc gccagcagca agtgctcgac acttacgaac
     8161 atatctttca tgttttgggc ttctttttca gccctgagga aaaccctggc gagccccgtg
     8221 ttcatggcca ggggcccgtc gtagccgtga acggaaggta tccgatttac tgcgtcttcg
     8281 gcaagggact tgagcagatt taagtcacat ccgatttttt gcatgagacg ggggatgatg
     8341 ccgtcttctt gacggagtag ggcgtacatt aggtgccttg aagtcacttc ctggtgccga
     8401 tgctctacgg ccaaatcctg ggcttttaaa atggcttcct gtgatttttg cgtatattta
     8461 cttatatcca taatatacac cttctttcag tttttataat acaacaaagg tcaggcaagg
     8521 tcaattactt cacacaagtt aataagttag gtcccggccc cttgccttgc cggcttctat
     8581 ttgttacaat aaaataaatc gagtttttga ggtgccaaat gaggaaagtg ttttttaact
     8641 aattaatcaa atagatccta aagagcatag gtgaaccctt aagctttggc caggggacca
     8701 gtatgccctt tttaggatgg attagttaaa aaacacttaa gtaacttaga acgaatttat
     8761 attatatgtg atgcaggaac tttggtaaaa tccgcatcat atctatataa atactatgca
     8821 atttactgca gcattacact taggtgttag ctgcagtttt tattttgtgt gaaaggtata
     8881 gttttcgtta atcgcagagt gttgtctgca ctgcagggca tgttcgtata tttttacttg
     8941 gaatggggat gttttatgaa tataaatttt actgagcttt caaagggcta caagggaaaa
     9001 atggttttta aaaaaatcag cggccaaata actgatgggg atagagtggg tttggttggt
     9061 gcaaacggtg ttgggaagac gactcttgcc aagctcctgg tgggcttgga agaaaaagat
     9121 agtggcacta ttacttactc gccgcctcat atgaagcttt tatatgtaga gcaatatccg
     9181 gtctttgata aagatgtcac cgtttatgag gaggctatta gaattgcgtt aaagtttaac
     9241 ggcaataggg caaatgttgc cgccattgct aaaaagggcc tttcaaaagt tggcttgaaa
     9301 gaagacaagt ggaagcaaaa agcaatgagc ttaagcggag gggaaaaaac caagctggcc
     9361 ctttataaag cattagctgc agactttgat ttactcatac tcgatgaacc cacaaaccac
     9421 ctggatgcga aaagcttgga gtggctggag gaatttgtct taagtattga aaaaccaatt
     9481 ttagtgattt cccacgatag atttttcctg gataaagttg caaataagat ttgggagtta
     9541 acttctcagg aattgaaggc ctatgagggg aattattcat catataaaga gcaaaaagaa
     9601 attgagataa aaagtctaac aagagaatac aataaggagc agatgaggat ttctcaatta
     9661 aaagaagaaa taaatgacag gaggaactgg tataacagtg cccataaggg ggcgggcacc
     9721 aacgattttt acaggtctag ggccacaaag catgcaagag ctttaaaagc caaacaaagg
     9781 cagcttgaaa gaattgaaaa ggagaaaatt gaaaaaccca aaaaaccggt gtcgcctgct
     9841 tttgatatta tcaataaaaa tattattggt agaaagtttc ctaggtttct cgtccagggg
     9901 aaaaacctgg ccaagaagta tggcaacaag caaatctttc aagacgttac gtttaacata
     9961 caacaccagg ataaaatcgc attgctcggt gaaaacggtg ttggcaagac aactctgctt
    10021 aaaataatat gcggtttgga taaggacttt tgcggcaatc ttaagataaa cccttctgtt
    10081 aaaatcggct actttgcaca ggagcttgac aacttaaaca gtgaagctac agtacttgat
    10141 gatgttctgc ttgaaggtaa tacggttgaa gagactcgcc tgctgcttgc cggtttactt
    10201 tttagagggg acgatgtgtt taaaaagata cggaatttaa gcatgggaga aaagggcagg
    10261 gtagcctttg caaaactgat tttatcaggt gccaacttgt tggttttaga tgaagccacc
    10321 aattatatgg acatagtatc aaaagaaaaa atcgaagagg tactggagga attaaggggc
    10381 agcattatct ttgtctccca cgacagatat tttatcaggc gtttggcaaa taaagttttt
    10441 acaatagaga atagaaagct ttactgcttt gaagggaatt acgattatta cttggccaaa
    10501 cgcaaagatc aacaggcgca aaaagaaatc gggacagatt atcgcaccgt agctgaaaat
    10561 atcaagcgcc tggaactgga gctggccttt ttatcaggta aactagatga aaccgtggat
    10621 gaagaagaga aagagaagtt aaatgaaaga tttttagccg tggcacgtga acttaacaaa
    10681 aacagggagt tgcttaaact gtaa
//
LOCUS       DAONUO010000024        10682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4773_length_10682_cov_3.633104, whole genome shotgun sequence.
ACCESSION   DAONUO010000024 DAONUO010000000
VERSION     DAONUO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4773_length_10682_cov_3.633104"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /gene="hemW"
                     /locus_tag="PK768_01565"
     CDS             complement(<1..942)
                     /gene="hemW"
                     /locus_tag="PK768_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQA59688.1"
                     /translation="MKSMKTIGLYLHIPFCIKKCNYCDFTSYESLEQVPEYLAALKKE
                     ISNLEKHKFSVNTLYIGGGTPTVLSENQLFSLLNVLHKSIHIAKEAEISIEANPGTLT
                     REKLCLLKKLGINRLSIGLQACQNSLLQTMGRIHTVEEFESNFEAARDTGFDNINVDL
                     IFGLPGQSPEDFEKTLTHVLSFSPEHISCYALSVEEGTKFYEWQKEGRLVLPSDDEER
                     KMYHKAIEILTHEGYEHYEISNFSLPGRECRHNLTYWTYEEYLGLGAGAHSFFDNKRF
                     YNHPGLSEYIESINMSSSAIANIELISEKEQQAEFCFM"
     gene            complement(948..2750)
                     /gene="lepA"
                     /locus_tag="PK768_01570"
     CDS             complement(948..2750)
                     /gene="lepA"
                     /locus_tag="PK768_01570"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQA59689.1"
                     /translation="MSIPRERIRNFCIIAHIDHGKSTLADRLLEYTGTISNRKMEEQV
                     LDKMDLEKERGITIKAKAVRMIYRADDGYDYLLNLIDTPGHVDFSYEVSRSLAACEGA
                     LLVVDASQGIEAQTLANTYLALENNLEIIPVINKIDLASADPEEAKREIEEIIGLDAQ
                     NAILTSAKEGQGIKEVLEAIVKLVPPPEGSSDKPLRSLIFDSMYDSYKGAVAFVRIMD
                     GSIRPRQLIKMMSTGKTFEVTEIGVFNPDMQPVDYLEAGQVGYVAASIKNVIDTRVGD
                     TITDAQKPAKQALPGYRKAVPMVFCGMYPAEGEEYENLKEALGKLQLSDASLFFEPES
                     SAALGFGFRCGFLGLLHMDVVQERLEREYNVNLITTAPSVIYKITKTDGSTIDVDNPT
                     NFPNPAEIECIEEPYVEASIMLPTEYVGPVMELCQEKRGSMKNMEYINERRVILRYEM
                     PLSEIIYDLFDQLKSRTRGYASLDYELIGYRKSDMVKLDILLNGEVVDALSFIVHKDK
                     AYSRGRQIAEKLKEVIPRQMFEVAIQAAIGGKIIARETVRALRKNVLAKCYGGDITRK
                     KKLLEKQKEGKKRMRQIGSVQVPQEAFMAVLKME"
     gene            complement(2783..3127)
                     /locus_tag="PK768_01575"
     CDS             complement(2783..3127)
                     /locus_tag="PK768_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQA59690.1"
                     /translation="MIYKDLITLIIIFFLLLALGVRIAEQGLYETMGLDLRPKSFDFD
                     FQRDRVYSFTILGNSLEVKKYYQVGNIVACREKIDVTINGKSFTINPIIPTGVMMRES
                     VNLDKKTAKMYN"
     gene            complement(3195..4346)
                     /locus_tag="PK768_01580"
     CDS             complement(3195..4346)
                     /locus_tag="PK768_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HQA59691.1"
                     /translation="MIRIKVIRMRSILLSVIVLISILSFLSFLLANYFHSRAVSVIAG
                     MQNESRQSFIHKIDSDIFKGMLTLSLPALEAPDQEYIFIDFRNLMGSLSKTLTNVDVL
                     NPETYFFIHLPLISMIGTEAAMTPGFELPQGPQIYEDEHLPSENLPPPEIKEAEETEI
                     EKLVNISEGPLVLIYHTHTTEGYMPSEKFNYKPDSTYHTRDLNFSVAKVGELMTEELN
                     RLNVPTIQDKTFHDIPTYMTSYANSLKTVEQILKQHPSIKIVIDLHRDAPVPDLQKSR
                     EITTVKIDDSTYSRFLLVVGTDRTFPHPNWKENYRFATLLHDRLEQLYPGISRGIDLR
                     SERFNQHLSNKAILVEIGSHGNTMEESLASAKIFAQVLAEIVKELSITE"
     gene            complement(4415..5425)
                     /gene="gpr"
                     /locus_tag="PK768_01585"
     CDS             complement(4415..5425)
                     /gene="gpr"
                     /locus_tag="PK768_01585"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HQA59692.1"
                     /translation="MPLCQPNFDKEAIGINIRTDLALEAREMVGEEQLPGVIVETEES
                     EQMTISRVRIKDEQGAKAMGKPMGYYVTLEVPRLKEKDPALTEEVSKNLADELERMVE
                     IPRDATILVVGLGNWNVTPDSLGPKVIEKLIITRHLLEHMPGKFSPNSGIRPVCALAP
                     GVLGITGIETGDIIRGIVEKVKPNMIIAIDALASRSMERISTTIQIADTGVYPGSGVG
                     NKRMGITRETMGVPVIAIGVPTVVDAATMANDTLDMLIGQFARQATPGSEFYKLLEEL
                     DKEEKYKLIREVMTPFVGQLVVTPKEIDALIDNTAKTLAGGLNLALHPGIEYDEVSTF
                     LQ"
     gene            complement(5397..5723)
                     /locus_tag="PK768_01590"
     CDS             complement(5397..5723)
                     /locus_tag="PK768_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQA59693.1"
                     /translation="MIGMIIRFIVSALVLMLVSFILPGFNVSGFTGALIAAIIIAILG
                     FIAESLLGDKVSPQSRGIVGFITSAVVIYFAQFLVPAMSVTWWGALLASLVIGVIDAF
                     VPTELR"
     gene            5890..6162
                     /gene="rpsT"
                     /locus_tag="PK768_01595"
     CDS             5890..6162
                     /gene="rpsT"
                     /locus_tag="PK768_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQA59694.1"
                     /translation="MANTASARKRVRQTKKRTLRNNKVKSQIRYSVKRFMEALNAADP
                     ENIQSALRYAVKMIDKAASKGVIHKNTAARKKASLYKRLSEANTAS"
     gene            complement(6391..7353)
                     /gene="holA"
                     /locus_tag="PK768_01600"
     CDS             complement(6391..7353)
                     /gene="holA"
                     /locus_tag="PK768_01600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQA59695.1"
                     /translation="MKNVILLFGEEKLLINEEIDKIKKKLVPGHLETMNFIPIDGKNV
                     PQEEILNACQTVPFLSQKKLVVVYDAPFFESGKGDAEKSDKQDDLLYRLQTIPDYTVL
                     IFSASKVDKRKKIYKLIKSKGIAREFNQLSIKDKAFWIQKRAKLYGKNIDLKTAYFIA
                     EHTGDLNQTNNELKKIAAFIGDKGSIESEDLSKIFYKSLEGNIFEMMDYIGMKNPSNA
                     IGILNHLTEQGEKAVVILYMISKHLMDLMTVKSMEGLNYQDILSKSGLHPFVLKKALK
                     QAKNFTLDELNRNLKLCQQLDSDIKTGKIQEMLGLELLIAEISS"
     gene            complement(7350..9695)
                     /locus_tag="PK768_01605"
     CDS             complement(7350..9695)
                     /locus_tag="PK768_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQA59696.1"
                     /translation="MYRPFLFIATYFVFGVALGNFMKAAWMYFFSTIICLLATVFCKN
                     KKAKVAFLAASLVFFGAFYYCFRLDRIPGTIVDFAGEQQTIVGIISDTLTTKNDRVVY
                     DVKTLYIKANEGYKTITGKVRLSSSLEDKSQMLRYGDVIEFSGKLKLPQGRRNPNGFD
                     YNAYLLQKGISTTMFSREIEVIGKGKTNAFKAAAFYLRERLVAFFESHLPPNISSLIV
                     GITLGIKDNIPGETIKAVKDSGVAHAFAVSGLHTGVIYGALELFLNKLGASRKISFVI
                     ESIAIIFYSFMAGLSPSVMRASIMIMVFMLSKVIGRENDSLNSLCFSAVILLLINPLI
                     LFSVSFQLSYAAVIGIVLFYNPIKNKLNALPKYLNESIAVMVGAQLVVAPILAYYFYN
                     LSLIGFITNLLVVPLVSFILIGGIISGITGLFFEPLGAIFIKFPGFLLELVENIVLVS
                     SRLPFATVIVPAMPLISTFLYYISLALIFDLLSLKSEYYIKHKKAIAILLIVIAVLPG
                     LWPFRAFEVTFIDVGQGDSLLIQTAGGKVILIDGGGIPSYTNSDFDTGEDIVLPLLYS
                     KGIKQIDIAVFTHFDDDHAKGLLSILKSMRVKNIVYGLPEDGALYNEMLEIAQQKGIK
                     TIQVARGDSFIVDNAVFDVLHPVKYAPFSSGNNNSIVLKMSYNGVSFLFTGDLEYEGE
                     QSLISSRSDIGAHILKIGHHGSSTSTSEEFLSRVNPMYAVITVGVDNNFGHPSAKVID
                     LLEERGIAVFRTDINGAISFKIFKDNVKIYTTISGREYIKP"
     gene            complement(9947..10549)
                     /locus_tag="PK768_01610"
     CDS             complement(9947..10549)
                     /locus_tag="PK768_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HQA59697.1"
                     /translation="MVHNQPPVMIKTRYLKKERLDVEYPSIEGLFNKNAEHYINSVLI
                     SIVNTLIEQTDYFQNPMTEVTGRYYIRTNNKGLLGISVEMYWFFGGAHGMTVLKSVTF
                     NVTTGRIYRLQDLFKESSDYVKRLTDIIKRQIQERDIPVIVDFTSIKPDQDFYIENRT
                     LVIYFQLYELTPYAYGFPTFIIPTQEIDDILIEDILSRGE"
BASE COUNT         3003 a         2337 c         1990 g         3352 t
ORIGIN      
        1 catgaaacag aattctgcct gctgttcctt ttccgaaatc agctctatgt ttgctatggc
       61 agaagaagac atgttgatgg actcgatata ctccgaaagg ccgggatgat tgtaaaagcg
      121 cttgttgtca aaaaaagagt gtgctccggc gccaaggcct aaatactcct cgtaagtcca
      181 gtatgtcaaa ttgtgcctgc attctcggcc gggtagtgaa aaatttgata tctcgtaatg
      241 ctcgtagccc tcatgagtca gaatctcaat tgctttatgg tacatctttc gctcttcatc
      301 atcagaggga aggacgagcc ttccctcttt ttgccactcg taaaacttgg ttccctcttc
      361 aacagacaaa gcataacacg agatgtgctc cggtgaaaaa gacaaaacat gtgttaaagt
      421 tttttcgaaa tcttcagggc tctgaccggg taggccgaag atcaaatcca cattgatgtt
      481 atcaaatccc gtatcacgag cagcttcaaa attgctttca aattcctcca cggtgtgaat
      541 tctgcccatt gtctgaagaa gtgagttttg acatgcctga aggccgatgc taagtctatt
      601 tatgcccagc ttctttaata ggcacagctt ttcccgagtc aatgttccag gatttgcttc
      661 aatgctaatc tccgcttcct ttgctatatg aatactcttg tgcagcacat ttaacaagga
      721 aaatagctgg ttttccgata agacggtggg agtcccccca cctatataca gggtatttac
      781 actgaatttg tgcttttcca ggtttgaaat ctcctttttt agggctgcaa ggtattccgg
      841 tacttgctct aaactttcat aggaagtaaa atcgcagtag ttgcattttt taatgcaaaa
      901 aggtatgtga aggtacaggc cgatggtttt catgcttttc atgctttcta ctccattttc
      961 aaaaccgcca tgaaggcttc ctggggcacc tggactgagc ctatttgacg catgcgcttt
     1021 ttgccctctt tttgcttttc taaaagtttc tttttccgtg taatatcgcc gccgtaacat
     1081 tttgccagca catttttcct aagcgccctg acagtttccc gggctatgat cttgccacct
     1141 atggccgctt gaattgcaac ttcaaacatc tgtctgggga ttacttcttt caatttttcc
     1201 gctatctggc gtccacggct gtacgcctta tctttgtgta cgataaatga aagtgcatcc
     1261 acaacttctc cgtttaacaa aatgtccagt ttaaccatat ctgatttcct gtagccgata
     1321 agctcgtagt ccaaggatgc gtaacctcga gttcttgatt ttaattggtc aaacaggtcg
     1381 tagattattt cagaaagggg catctcatac cttaaaatca ccctgcgctc gttgatgtac
     1441 tccatattct tcatactgcc ccgtttttcc tggcaaagtt ccataactgg ccccacatac
     1501 tcggtaggca gcattatgga agcttccaca tagggttcct ctatacattc tatttcggca
     1561 ggattgggaa aattagtggg attgtccaca tcaattgtgc tgccatcagt tttagttatt
     1621 ttgtagatta cgctaggagc cgttgtaata agatttacat tgtattccct ctccaaccgc
     1681 tcctggacaa catccatgtg cagtaagcct aaaaagccgc acctaaaacc aaaaccgagg
     1741 gctgcggagc tttcaggctc aaaaaataag gatgcatcgc tgagctgcag tttgcccagt
     1801 gcttctttta agttctcgta ttcttcacct tccgcgggat acataccgca aaagaccatg
     1861 ggcacggcct tcctgtatcc cggcaaagct tgtttggcag gcttttgagc atcggtgatg
     1921 gtatcgccta ccctcgtatc gataacattt tttatgctgg ctgcaacata gccaacctgg
     1981 ccagcttcaa ggtaatcaac gggctgcatg tcaggattaa aaacgccgat ttccgtcact
     2041 tcaaaggtct tgcccgttga catcatctta atcaactgcc tgggtcttat gctgccgtcc
     2101 attattcgaa caaaagccac cgcccctttg tacgaatcat acatggagtc aaaaataagg
     2161 gaacgcaagg gcttgtcgct ggagccttca ggtggcggga ctagcttgac aatcgcttcc
     2221 aggacttcct ttataccctg accctccttt gcggaagtga gtatcgcatt ttgggcatca
     2281 aggcctataa tttcctcgat ttcgcgcttt gcctcttcag ggtcagcact tgccaaatct
     2341 attttattta taaccggtat gatttctaaa ttgttctcaa gggctaggta agtatttgca
     2401 agggtttgcg cctctatacc ctggctggca tctaccacaa gcaaggcccc ctcgcaggct
     2461 gcaagacttc ttgacacttc ataactaaag tcaacatggc cgggcgtatc tatcagattt
     2521 aaaaggtaat catacccgtc atcggctcga tatatcatcc taaccgcctt ggctttgata
     2581 gtaatgcctc tttccttttc caaatccatt ttatcgagaa cttgctcttc catttttctg
     2641 ttagaaattg tgcctgtata ctctaaaagc ctatctgcca gagtggattt gccgtgatcg
     2701 atatgagcga taatgcaaaa attcctaatt ctctcccgag gtattgacat cgtgttcctc
     2761 ctcttaattc tcagctttaa attcaattat acattttggc agttttttta tcaagattca
     2821 cggattccct catcattacc ccggtaggaa ttataggatt gattgtaaaa cttttcccat
     2881 taatagtaac atctattttc tccctgcagg ccactatatt tccaacttga taatattttt
     2941 taacttctag gctgttgccc aagatggtaa agctatatac ccggtccctt tgaaaatcaa
     3001 aatcaaagct cttcggtctc aaatcaaggc ccatggtttc atacagacct tgttcggcaa
     3061 tgcgaacccc gagagcgagc aaaaggaaaa aaataatgat gagcgtaatt aggtccttgt
     3121 aaatcatgct ccagttcctt tacctgcgat atgataagct ttgcatgatt tatcttaaac
     3181 aaattatctc tttttcattc agttattgat aattctttta ctatttcagc caaaacttgt
     3241 gcaaaaatct ttgcagacgc aagggattct tccatagtat tgccgtggct gccaatttct
     3301 actaggatag ctttgttaga tagatgctga ttaaagcgct cggatctcaa gtcaatgcct
     3361 cgggatatgc ccggataaag ctgttccaac ctatcatgta gtagcgttgc aaatcgataa
     3421 ttctccttcc aattgggatg gggaaaagtc ctatccgttc ccacaacaag caaaaacctg
     3481 gaatatgtgc tgtcatctat cttgacagta gttatttccc tggacttttg caggtcaggt
     3541 accggtgcat ccctatgaag gtcgataact atttttatgg atggatgctg ttttaagatc
     3601 tgctccacag tttttagtga gttggcatat gatgtcatat atgtaggaat atcatggaaa
     3661 gttttgtcct gaattgtggg cacgtttagt cgatttaatt cttccgtcat cagttctccc
     3721 acctttgcaa cgctgaagtt taaatccctg gtgtggtaag tggaatccgg tttataattg
     3781 aatttttccg atggcatata gccttcagtt gtatgagtgt ggtatatgag caccagcgga
     3841 ccttctgaaa tattcacgag cttctcaatt tcagtctctt cagcttcttt tatttccggc
     3901 ggcggtaggt tttctgaagg taagtgctca tcttcgtata tttgggggcc ttgtggcagc
     3961 tcgaaacccg gtgtcatggc tgcctcagtg cctatcatgc taattagcgg caggtgaata
     4021 aagaagtagg tttcaggatt gagcacatcg acattagtaa gtgtcttgga aagactgccc
     4081 atcaggtttc tgaaatcgat aaagatatac tcttgatcag gagcttcaag tgcgggcagg
     4141 ctcaaggtca gcattccctt aaatatatcg ctgtcaattt tgtgtataaa actttgtcgg
     4201 ctttcatttt gcatccctgc aattacagaa accgccctgg aatgaaaata atttgcaagg
     4261 agaaaagata aaaagcttaa aattgatatc aatacaatga cagataacag aatgcttctc
     4321 atccttatta cttttattct tatcaagccc atcacatcct ctcaataagc atattcacgt
     4381 acataaaaaa aaatgaggca aatatttagc ctcactactg caaaaaggta cttacttcat
     4441 cgtattctat ccccggatga agggctagat ttaaacctcc ggccaaggtt ttagcggtat
     4501 tgtcaatcag ggcatctatt tcctttggtg taacgaccag ctggcctaca aagggtgtca
     4561 taacttcgcg aattagcttg tatttttcct ctttgtccag ttcttcaaga agcttataaa
     4621 actcactgcc gggagtagct tgccttgcaa actggccgat gagcatatct aaggtgtcat
     4681 tggccatggt ggcagcatca actaccgtgg ggacacctat agctataaca ggcactccca
     4741 tggtttcacg ggttataccc atccgcttgt tgccaacacc ggagccggga tatacgcccg
     4801 tatcagcgat ttgtatggta gtgcttatgc gctccatgct cctagatgct aaggcatcta
     4861 ttgcgatgat catgttaggc ttgaccttct caactatgcc acgaatgata tctcccgtct
     4921 ctatcccggt tattcccaaa actcccggag ccagtgcaca cactggacgt ataccactgt
     4981 tgggactaaa cttccccggc atgtgttcaa gtaagtgcct tgtaatgatt agtttttcta
     5041 ttacctttgg ccccagagaa tcgggggtga cattccagtt gccaaggcct acgacgagaa
     5101 tagtagcatc tctagggatt tcaaccattc gctcaagttc atcggctaga tttttactca
     5161 cttcttccgt aagggcagga tccttttctt ttaacctagg cacttccaag gtcacgtaat
     5221 aacccatggg cttacccatg gccttagccc cctgctcatc cttaatccta accctgctta
     5281 ttgtcatttg ctctgactct tcagtttcaa ctattacgcc gggcagctgt tcttctccca
     5341 ccatctctct tgcctctagg gcaaggtcag tgcgtatgtt aataccaatc gcctccttat
     5401 cgaagttcgg ttggcacaaa ggcatcgatc acacctatga ccaaggatgc cagcaacgct
     5461 ccccaccaag ttacgctcat ggcaggaact aaaaactggg caaaataaat tacaactgct
     5521 gaagtaataa aacctacaat ccctcgactt tggggagaga ccttgtcacc gagcaagctt
     5581 tcggctataa agcctaaaat tgcaataatt atggcagcaa ttaaagcacc tgtaaagccc
     5641 gatacattaa agccgggtag aataaagctg acaagcatta ataccagggc tgaaacaata
     5701 aaccttatta tcattcctat cataaaactg cacctccata tttttttcat ttattatatt
     5761 aaccgctaac aatttataat atacgataga ggcttttcat cattttttat taatgttgca
     5821 gacttgcctt gcattttggc aagcgaagtg ataaaatatc cctagtattt tttagggggt
     5881 gaaggaattt tggcaaatac agcttcggcc aggaaacgcg tgcgccagac gaagaagcgg
     5941 acgcttcgga ataataaagt taaatcacag ataagatact ctgtaaaaag gtttatggaa
     6001 gccttaaatg cagcggatcc tgaaaatatt caaagcgcct tgagatatgc cgtaaagatg
     6061 attgacaaag ctgcatccaa gggcgtcatt cacaaaaata ctgctgcccg caagaaagcc
     6121 agtctgtata aaaggctttc agaagcaaac acagcaagtt aaactagcgt aagccagagc
     6181 ttacgcattt tcttttttat ccagcataaa tatttcattt ggattaatat ttccctacga
     6241 aaaaagcttc caagccggat acctttgctt tggggacaca aaaacgctcc cccttgtagt
     6301 aaaatccaaa agaggagcat ataattcccc ggactcggaa gcttttttcg tgtgcttgtt
     6361 tgtttaattc ggcttttttt gccttgtcct ttaagaagat atttccgcaa tcaaaagttc
     6421 cagacccagc atttcctgga ttttgcccgt cttgatatcg ctatccaatt gctggcatag
     6481 tttaagattt ctatttagtt catccagagt aaaattcttt gcctgtttta acgctttttt
     6541 cagaacaaag gggtgtaaac ctgactttga caatatatct tgataattta aaccttccat
     6601 gctttttacc gtcatcaaat ccatcaaatg ttttgatatc atatacagga tgacgacagc
     6661 cttttcgccc tgctctgtta aatgatttag tattccgatg gcattcgagg gatttttcat
     6721 gccgatataa tccatcatct caaatatatt gccttccaat gatttgtaga agatctttga
     6781 caaatcttca gattcaatac ttcctttgtc acctataaaa gcagcaattt ttttcagctc
     6841 attatttgtt tggttcaaat ctccggtgtg ctctgctata aaataggcag tctttaaatc
     6901 tatgtttttg ccataaagct ttgccctctt ttgaatccaa aatgccttgt cttttatgga
     6961 aagctgattg aactcgcggg ctataccttt tgatttgatt agcttatata tctttttccg
     7021 cttatcaact tttgatgcgc taaaaatcag tacggtatag tcgggaattg tctgaagtct
     7081 atataacaaa tcatcctgct tgtccgactt ttccgcatct cctttcccgc tttcaaagaa
     7141 aggtgcatca tatactacca ccaatttttt ttgtgaaagg aagggaactg tttggcaggc
     7201 atttagtatt tcttcttgag gaacattctt gccgtcgatg ggaataaagt tcatcgtttc
     7261 caaatgacca gggacaagct tttttttgat tttgtcaatt tcttcattga ttaagagttt
     7321 ctcttctcca aaaagaagta ttacattttt catggtttta tgtactccct accggatatg
     7381 gtcgtatata ttttaacatt gtctttaaag attttaaagc ttatagctcc attaatgtcg
     7441 gtcctaaaaa cggcaatgcc cctctcctct aatagatcta ttacctttgc tgagggatgg
     7501 ccgaaattat tgtctactcc aacggttata acagcgtaca ttggatttac ccttgataga
     7561 aactcctcag atgttgatgt ggagcttcca tggtggccga tttttaaaat gtgggcccca
     7621 atatcacttc ttgagcttat gagactttgc tctccttcat attctaaatc acctgtgaat
     7681 aaaaaactta caccattgta actcatttta agcacaatcg agttattgtt gccggaagaa
     7741 aagggcgcat acttaacagg atgcagtaca tcaaaaactg cgttatccac aatgaaactg
     7801 tctcctctgg ctacctgtat ggttttgatt cctttctgtt gtgcgatttc aagcatttcg
     7861 ttatataggg caccatcttc cggcagcccg tacacaatgt ttttaactct catggatttt
     7921 aatattgata gcaagccttt ggcatggtca tcgtcgaagt gcgtaaaaac agcaatatca
     7981 atttgtttta ttccttttga ataaagcaaa ggcaaaacta tatcttctcc cgtatcaaaa
     8041 tcactgttag tatatgacgg gataccgccc ccatcaatta atatgacttt accgcctgca
     8101 gtttgaatta gtaaagaatc accctgacct acatcgataa aagtcacctc aaaggctctg
     8161 aaaggccata ggccaggcaa tactgcaatt actattaata aaatggcaat tgcttttttg
     8221 tgttttatat agtattcgct ttttaaagat aataagtcaa agattaatgc cagggagata
     8281 taatacaaaa atgtgcttat aagtggcatc gcaggtacta ttacggttgc aaaggggagc
     8341 cttgaactta cgaggacaat attttctacg agttctaata gaaagcccgg aaattttata
     8401 aaaatggccc ccaaaggttc aaagaaaagt cctgtaattc cggaaataat accgccaatt
     8461 aatataaagc tcactaaggg caccactaga agattggtta tgaaaccaat tagagataaa
     8521 ttataaaaat aataagccaa aatcggtgct accactagct gagctcctac catcactgca
     8581 attgactcat tgagatattt tggtagggca ttaagcttat tttttattgg attataaaaa
     8641 agaactatac cgatgactgc agcgtatgaa agttgaaaac tgactgaaaa tagaattagg
     8701 ggatttatta gcagcaggat cactgccgaa aagcataagc tgtttaacga gtcattttcc
     8761 cttccaataa ctttagacag cataaatacc attatcatga tggaagccct catcacagaa
     8821 ggtgataggc ctgccataaa actataaaaa attattgcta tgctctcgat gacaaatgag
     8881 atttttctgg aggcgcctag tttattaaga aataactcaa gggccccata tatgacacca
     8941 gtatgaaggc ctgacactgc aaaggcatga gcaactccgc tatcttttac cgctttaatg
     9001 gtttctcccg ggatattgtc tttaattcct aaagtgatac caacaatcag cgatgatata
     9061 tttggtggaa gatggctttc gaaaaacgca actagacgct cccttaagta aaaggcagct
     9121 gctttgaaag cattggtctt gcctttgcct attacttcaa tctcccgtga aaacatagtg
     9181 gttgagatac ccttttgcaa aagatatgca ttgtagtcaa aaccatttgg atttcgacgg
     9241 ccctggggca gtttcaattt cccggaaaac tcaatcacat ctccgtagcg cagcatctgg
     9301 cttttatctt ccaagctcga agaaagcctt actttcccgg taatggtttt gtatccttca
     9361 ttggccttta tgtataatgt ttttacatca tatactaccc tgtcattttt ggttgtaagt
     9421 gtatcgctta taatgcccac tatagtttgc tgttccccag caaaatcaac aatggtacca
     9481 gggattctat ccagcctaaa acaataataa aaagctccga agaaaactaa agaggcggcc
     9541 aaaaatgcca cttttgcttt cttgttttta caaaatactg tggccaaaag gcaaataatt
     9601 gtcgagaaaa aatacatcca ggcagccttc atgaaattgc ccaaggctac tccaaaaaca
     9661 aaatatgtgg caataaacaa aaaaggccta tacataccct gctccttgca caaacatttt
     9721 ttgtcagtat atccataatc caaattttcg tacctgctaa ataacgttaa tgacttttag
     9781 ctgtatgttt aaccctttgg catcatactt tattttatca gatgtagcta taatttttct
     9841 actctaaaac ttattgagct ttggcttctc gaacttatga ggttaagttg caataaaaga
     9901 tccaagggaa tttaattatc gccattgccc ccttggattt tctcaattac tcgcctctgc
     9961 ttaatatgtc ttctatcaaa atatcatcaa tttcctgggt aggaattata aatgttggaa
    10021 aaccgtatgc ataaggtgtc agttcatata gctggaaata tataactaga gttctgtttt
    10081 caatataaaa gtcctggtct ggctttatac tggtaaaatc aactattaca ggtatatctc
    10141 tctcctgaat ctgtcgtttt attatatctg tcaagcgttt cacataatca gagctttctt
    10201 taaacagatc ctgcagacgg taaatcctac ctgttgttac attaaaagtc acggatttta
    10261 aaactgtcat accgtgtgct ccgccaaaaa accaatacat ttcaacagaa atacccaaaa
    10321 gtcctttatt gtttgttctt atataatatc ttcccgtcac ttcggtcatg ggattttgga
    10381 aataatctgt ttgttctatc aatgtattaa ctatgcttat caacactgaa ttgatataat
    10441 gctctgcatt cttgttaaac aaaccttcaa ttgaagggta ctctacgtcc agcctttcct
    10501 tttttaagta acgagtctta atcattacag gtggttgatt atgcaccatg atgaatcacc
    10561 tctcaaaaat aaaatgccac tttatttaat ctattccggt ataaaacttt ttgttaaaac
    10621 aaaaaacacc tatcgaaagg tgttttgtag aaaaccgtat cttaggatta caatataatt
    10681 aa
//
LOCUS       DAONUO010000025        10602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4816_length_10602_cov_3.691002, whole genome shotgun sequence.
ACCESSION   DAONUO010000025 DAONUO010000000
VERSION     DAONUO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4816_length_10602_cov_3.691002"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /gene="ispD"
                     /locus_tag="PK768_01615"
     CDS             complement(<1..526)
                     /gene="ispD"
                     /locus_tag="PK768_01615"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQA59698.1"
                     /translation="MHISAIIAAGGQGRRMNSSVSKQFLNIKGHPILYYTLNKFEKMD
                     ELNSIVLVTGEADIDYTREKIVKRYRFKKVELVSGGKERQDSVYNGLRKLSPQTDIVV
                     IHDGVRPFVPRQIIESSIAAAVEYKAVGVAVPVKDTIKVVDSDNTIKSTPDRSALWAM
                     QTPQTFHYDIIMKAY"
     gene            complement(544..1632)
                     /locus_tag="PK768_01620"
     CDS             complement(544..1632)
                     /locus_tag="PK768_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HQA59699.1"
                     /translation="MINKALRIIITIIGLGLGLQLTFLILFINNSIGLFRLDNVPTLI
                     IHITGTLLFGIIFYFLSSPIINVGMKAKEWIENRLQHTPMRDILYGFFGVLMGLLLAN
                     LFSRAFLSIPLIGGYMPIVINLIMAYFGGSIAIKKKEELSNLISFPIGNLSKKKKSPA
                     IINLVKPKILDTSVIIDGRIADICKTGFIEGTLVIPSFVLEELRHIADSSDSLKRNRG
                     RRGLDILNKIQKELDIEVEIYEGDFDDINEVDSKLVKLAQVLDASIITNDFNLNKVAE
                     FQKVQVLNINELANAVKPVVLPGEEMVVQVIKDGKESGQGVAYLDDGTMIVVDGGKKH
                     IGETIDVMVTSVLQTAAGRMIFAKPKSL"
     gene            complement(1757..2233)
                     /locus_tag="PK768_01625"
     CDS             complement(1757..2233)
                     /locus_tag="PK768_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HQA59700.1"
                     /translation="MFNIGDKVVYPMHGAGIIEAIEEKEILGEKQKYYVMRMPIGDMK
                     VMIPLKNIKEIGVRQVAGDDEIREVLNILHGEKSKMSANWNRRYRANMEKIRSGNIFQ
                     VAEVVRNLSIREREKGLSTGERKMLENAKNILVSEIVLSKNIKEEEALQLIENAFN"
     gene            2460..2855
                     /locus_tag="PK768_01630"
     CDS             2460..2855
                     /locus_tag="PK768_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQA59701.1"
                     /translation="MKDLSWDEFQDTVADCLIRHKSILDVLSKLHETSARVNRAISKS
                     VTSCGCIQINAKKQQVPSEIRLYELVSYMDTHTKGQLCDNCREIVELEIGNLLFYLAA
                     LCNILGLSLEDIMSAENEKLATLGIYNLS"
     gene            complement(2914..4257)
                     /gene="radA"
                     /locus_tag="PK768_01635"
     CDS             complement(2914..4257)
                     /gene="radA"
                     /locus_tag="PK768_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQA59702.1"
                     /translation="MKERRRFICQQCGAIAPKWMGRCPECNSWNSIVEEAVLPEKKLE
                     GPYRNPQLLQEIEYSEDERILTSIAELDRVLGGGIVPGSLVLIGGDPGIGKSTLMLQI
                     CGNLSRERNVLYISGEESEKQIKLRADRLGIRDGNLYIMAENNMNSIEVAIKELRPQV
                     AIIDSIQTVYYPELSSGPGSVSQVRESTVKSLSLSKETDTAIFIVGHVTKEGSLAGPK
                     LLEHMVDAVLYFEGERYHSYRLLRSVKNRFGSTNEIGIFEMRDKGLEEVSSPSKYLLS
                     GKLKDTAGSAVVCILEGTRPLLVEVQALVSRSGFNIPRRQTSGVDYNRAALLIAVVEK
                     KLGYMLGQEDIYINVAGGIKVEEPGADLAVVAAIASSFKNRPVSEDTVIIGEVGLAGE
                     VRAVSHVEKRIQEASKLGFAKAVIPYENAVDISGSAKIKLQGVKNIGEAVEAIIE"
     gene            complement(4402..6840)
                     /locus_tag="PK768_01640"
     CDS             complement(4402..6840)
                     /locus_tag="PK768_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQA59703.1"
                     /translation="MNGKFTQRAQKVIAYAQEEARRLNHNVVGTEHLLLGLIREGEGV
                     AARALANLGIDVSKVRSQVEAMIGAGPFPIQGPIGYTPRAKRVMELAMDESRKLGHNY
                     VGTEHILLGLIREGEGVAAQVLNNLGVSLDKAQKEVLNLLGDPKAQGGHKKTANTPTL
                     NQFGRDLTELAAEGKLDPVVGREKEIERVIQVLSRRTKNNPCLIGEPGVGKTAVVEGL
                     AQKIVEQDVPEILKDKRVVSLDMASLVAGTKFRGEFEERLKKVMNEIKQASNVILFID
                     EMHTIIGAGAAEGAIDASNILKPALARGEIQTIGATTLDEYRKHVEKDPALERRFQPI
                     TVDEPTVEDTIAILKGLRDRYEAHHRVKISDEALEAAAKLSHRYITDRFLPDKAIDLI
                     DEAASRVRLKTLTTPPELKEVEEELEGIRKEKDAAIKAQEFEKAASLRDKEQETKAKL
                     EQLKQEWQKDHKLDNTTVTEEDIAEIVASWTGIPVQRLAEEESERLLNMEKILHNRVI
                     GQEEAVVAISRAIRRAYAGLKDPSRPIGSFIFLGPTGVGKTELARALADAMFGDENAM
                     IRLDMSEYMERFAVSRMVGAPPGYVGYEEGGELTEKVRRRPYSVVLFDEIEKAHPDVF
                     NILLQILEDGRLTDAKGRTVDFRNTILIMTSNVGANLIQRQKVLGFEPVKDEEQDYAD
                     MKERVLSELKRTFRPEFLNRIDEIIVFHALNEEHIGSIVSLMVNEVNDRLTEQGIIIE
                     VTDKAKDLLAKEGFDPMFGARPLRRVIQKRIEDRLSEELLKGNIATGDTVVIDAKEDD
                     LTFNKKESAEVPGN"
     gene            complement(6850..7902)
                     /locus_tag="PK768_01645"
     CDS             complement(6850..7902)
                     /locus_tag="PK768_01645"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HQA59704.1"
                     /translation="MSLEDLMDTSRIKWINSPGPESDIVISSRVRLARNLNEVPFPHL
                     LSERQANQIVDDVYSALKENPVFSKDIKLYKMNELTAEQKTMLTEMHFISMDLANSER
                     GAVIINHDKTVSIMINEEDHLRIQVMLPGLQLEEALRQIDKIDDLLEEKLDFAFDEKI
                     GYLTACPTNVGTGMRASVMMHLPALTMMEKIGQILSTISQVGLVVRGVYGEGSQAKGN
                     LYQISNQITLGKSEEDIIQNVVSVARQILMSERKTRQNLSGETRLRLEDKILRSYGIL
                     TNAKLVTSQEAMSFISDVRLGVDMGIIKDLVPSVLNELFIMTGPAYIQEVSENATTPL
                     ERDIYRANFIRQKMQR"
     gene            complement(7914..8444)
                     /locus_tag="PK768_01650"
     CDS             complement(7914..8444)
                     /locus_tag="PK768_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HQA59705.1"
                     /translation="MLCEECHMRPATVHITKIVNNEKTEMHLCEECAKEKHLSLSSSI
                     GGFGFEDTGFSVAKLLSSFFEPSSKTPIGSSEEFKCGRCGLTFQTFSKTGRFGCSQCY
                     STFKNQMNPMLRRIHGKTFHVGKVPKRSGGQIRIKQEIDRLKRELQEAVNAEEYERAA
                     VIRDKIKELEQNQIGS"
     gene            complement(8447..8926)
                     /locus_tag="PK768_01655"
     CDS             complement(8447..8926)
                     /locus_tag="PK768_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HQA59706.1"
                     /translation="MANLSDMIENFIKRMFEDMPEGILEIQRNEMANYFQCAPSQINY
                     VLTTRFTLERGYYVESRRGGGGYIRIKKLKINEDEFLKDIIEYIGDSISGSDSKAILE
                     RLWEEDVITEREMKILNAAINKYNLPLCPQDRDMVRARIMKAVITALLDFEARKGGA"
     gene            complement(9059..9295)
                     /locus_tag="PK768_01660"
     CDS             complement(9059..9295)
                     /locus_tag="PK768_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59707.1"
                     /translation="MVRSKKKMHVGPTPDRVEDIHKKKVDSLIEDQLLDDTMYGEIIP
                     SLMGWTSSEDQEQRASRLVEISEELSTSKRKKKR"
     gene            complement(9392..>10602)
                     /locus_tag="PK768_01665"
     CDS             complement(9392..>10602)
                     /locus_tag="PK768_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQA59708.1"
                     /translation="GSDEEVIRKCRADEPFSALVFKTMADPYVGRLTLFKVFSGSLKS
                     DSSVYNVTQDKTEKFGQIYVMRGKKQENITEVFAGDIAAVAKLQHTTTNDTLGEKDNP
                     VVLKPIDFPKPVLTLAAAPKSSGDEDKISSGLQRLMEEDKTFEVTKNNETGQLLVSGM
                     GEIHLEVLGARLANKFGSEMVLDTPAVPYRETIRGTTKVEGKHKKQSGGRGQYGHVWI
                     EIQPLDLENEFEFEDKIFGGVVPKQYVPAVEKGIREALQDGVLAGYPMVGVKVILYDG
                     SYHPVDSSEMAFKIAGSMAFKKGAAEAKPVLLEPIMDVTVVVPDNYMGDIIGDLNKRR
                     GRVLGMEPKAGMQHIKAQVPMAEMFRYATDLRSMTQGRGSFSSSFSHYEEVPAPIAEK
                     VIAEAQKNKE"
BASE COUNT         2440 a         2692 c         2202 g         3268 t
ORIGIN      
        1 cgtacgcctt cataatgatg tcataatgga atgtctgggg ggtctgcatg gcccagagag
       61 cgctgcggtc aggggtgctt tttattgtgt tatcgctgtc aactactttg atggtgtcct
      121 ttacgggcac cgcaacccct acagccttgt attctaccgc agcagcaatg ctgctttcga
      181 taatttgcct cggcacgaat ggtctcactc cgtcgtgtat aacaacaata tcggtttgag
      241 gagatagttt cctcaaaccg ttatagacag aatcctggcg ttctttgccg ccactaacaa
      301 gttctacttt tttgaatcga tatcgcttta cgattttctc ccgggtgtaa tcaatgtcag
      361 cttcacccgt caccaataca attgaattta actcatccat cttttcgaat ttgtttaggg
      421 tgtagtagag tataggatgt cctttgatgt tcaaaaactg cttgctgacg ctactgttca
      481 tacgtcttcc ctgaccgccg gcagctatta tggcgctaat gtgcattgct tattcactcc
      541 aagttacaag gattttggct ttgcaaatat cattcttcca gcggcagttt gaagtacact
      601 ggtaaccatc acgtctatgg tctcaccgat atgttttttg ccaccgtcca ctacaatcat
      661 cgtgccatca tccaaatagg ctacaccttg accgctttcc ttcccgtcct ttatgacctg
      721 gaccaccatt tcttcgccgg gaagcaccac cggttttacc gcattcgcca gctcattgat
      781 attgagcacc tggacctttt gaaactcggc tactttattc aagttaaaat cattggtaat
      841 tattgaagca tcgaggactt gggcaagctt gaccagcttg ctgtcaactt cgtttatgtc
      901 atcaaagtcc ccttcgtaaa tctccacttc tatatctagt tccttttgta tcttgtttaa
      961 gatatcaagg cccctcctgc cccggttgcg cttgagactg tccgaagagt cggctatatg
     1021 tctcaattcc tccaacacga aggaaggtat aactaaggtg ccttcaataa agcctgtctt
     1081 gcaaatatcc gctatgcggc catctatgat aacgcttgta tcaagtattt taggctttac
     1141 caggttgatg atggcaggag atttcttttt cttgcttaaa ttgcctatgg gaaaggaaat
     1201 gaggtttgac aactcttcct ttttcttaat ggcaatgctt ccgccaaaat aggccatgat
     1261 taaattgatt acaatgggca tataaccccc tatcaggggt atgcttaaaa aagcccttga
     1321 gaacaaattg gccaacaaga gccccatcaa aacgccgaaa aatccataca gtatatcccg
     1381 catgggggtg tgctgcagcc tgttttcaat ccactcctta gctttcatac cgacattaat
     1441 aattggagaa cttaggaaat aaaatatgat tccaaaaagt aaagtgccgg taatgtgaat
     1501 tatcagagtg gggacgttgt ccaggcgaaa aagtcctata gagttgttta taaataggat
     1561 taaaaaggtc agctgcaaac ccaatccaag gccgattatt gttattatga ttcgcaaggc
     1621 tttatttatc aaccatgtca cctcctataa ttattatagc catatttaag cttacttaac
     1681 ttttcaatta ttatacaata aatcattaat taaatctata aaattggcta taggagctta
     1741 taaaaaaaaa tgcacttcaa ttgaatgcat tttcaatcag ttgaagcgct tcctcctctt
     1801 taatattctt gctgagaact atttcgctaa cgagtatgtt ttttgcattt tcgagcattt
     1861 ttcgttctcc cgtagagagg cccttctccc tttctcttat cgacaagttt cggaccacct
     1921 ctgctacctg gaagatgttt ccgctcctga tcttctccat attcgcccta tagcgcctat
     1981 tccaattggc cgacatcttg ctcttttcgc cgtggaggat gtttaacact tcccttatct
     2041 catcatcccc ggcgacctgc cgaaccccta tctcttttat attttttaaa ggtatcatta
     2101 ccttcatgtc accgattggc atgcgcatta catagtattt ttgtttttct ccgagtattt
     2161 ctttttcttc tatggcttca ataatccctg ctccgtgcat gggatatacc actttatctc
     2221 cgatattgaa catccaaaag ctcctcctcg aaaatcaact tgtattgtta taatagcata
     2281 tttgagggct tttgtcaatt taaataatta ttatactaca agatgtagtt agtgtcaatg
     2341 gtattttgcc cttttatttg ggagctttcg aatcgattaa aatccatgtt tgtttgacaa
     2401 atccattaaa agccgtctat aatatagtca gatagtatat ggcaaggggg agtataggtg
     2461 tgaaagacct tagctgggac gagttccaag acactgttgc tgattgttta ataaggcaca
     2521 agagtattct cgatgtactg agcaagcttc acgaaacctc tgcacgggtt aatagagcta
     2581 tatcaaagtc ggttacgagt tgcggctgca tacagattaa tgcgaaaaaa cagcaggtcc
     2641 ccagtgaaat tcgcctctac gaattagtat cttacatgga tacacacact aaaggtcagc
     2701 tttgtgacaa ctgtagagaa atagtcgagc tcgaaatagg aaatctcctt ttttacctgg
     2761 ccgctttgtg caatatttta ggcctgagcc tggaagatat tatgagtgct gaaaacgaga
     2821 agctggctac ccttggaata tataatttat cataggatat taaaatgaaa agggaacgga
     2881 aaaccgttcc ctaaagtttt ttatggtcca tctttattct attatggcct ctaccgcttc
     2941 cccgatattt tttacacctt gaagctttat ttttgcggag ccgctgatat ctaccgcatt
     3001 ttcgtaggga atcacggcct tggcaaagcc cagttttgac gcctcctgaa tccgcttttc
     3061 cacatggctg acggccctta cctcacctgc aagccccact tcgccgatta tcaccgtgtc
     3121 ttctgataca gggcggtttt taaaacttga agctatggct gccaccaccg ccagatccgc
     3181 cccgggctct tcaactttta tgccgccggc tacgtttatg tagatgtcct cctggcccag
     3241 catgtatccc aattttttct caactaccgc aatgagcagt gccgcccggt tgtagtccac
     3301 acctgaagtc tggcgccggg gaatgttaaa acctgacctg gaaactaggg cctgaacctc
     3361 gaccaaaagg ggcctggtcc cttccaatat gcacaccacg gccgagccgg cggtgtcttt
     3421 tagcttgcct gacagtaaat acttggatgg actgctaact tcttccaatc ccttgtctct
     3481 catttcgaaa atgcctattt cattggtaga gccaaagcgg ttcttgacgg accttaggag
     3541 cctgtaagaa tggtatcttt caccttcaaa gtataggacc gcatcaacca tgtgctcaag
     3601 tagcttcggt cctgccaaac tgccttcctt ggtcacatgg cccacgataa aaatggcagt
     3661 atcggtttcc ttggacaggc tgaggctttt tacggtagat tcacgcacct ggctgacgct
     3721 gcctggcccg gaggaaagct cgggatagta caccgtctgg atagaatcaa tgattgccac
     3781 ctgcggcctt agttccttaa tggccacctc tatcgaattc atgttgtttt ctgccattat
     3841 gtacaaattc ccatctctta ttcccaatct gtctgcccga agctttatct gtttttccga
     3901 ttcctcccct gatatgtaca ggacatttct ctctctgctt aaattgccgc aaatttgcag
     3961 catcagtgtt gacttaccga tgccgggatc cccgccgatt aatacgaggc ttccggggac
     4021 gatgccaccc cccaataccc ggtccagttc agctattgat gtcaaaatcc tctcatcctc
     4081 ggagtattca atctcctgca aaagctgcgg gttcctgtaa ggcccctcca gctttttttc
     4141 aggcagcacc gcttcctcca caatgctgtt ccaactattg cactcggggc agcgccccat
     4201 ccacttagga gctattgcgc cgcactgctg gcatataaag cgtcttcttt ctttcaaaat
     4261 gtcccctccc tgtttcttaa aaaaagaggc agacaaggcc ttgtgaaagc tcctcgctgc
     4321 ctctcatata tacaaagata cagatagact cttaaacttc cgataacaat aagttaatag
     4381 cttagtgcct ggcaccgctg cctagttgcc gggcacctct gccgattcct ttttgttaaa
     4441 ggtcagatcg tcttctttgg catcgatgac aaccgtatcc cctgttgcaa tgttgccctt
     4501 taagagctcc tctgagagcc tatcttcgat gcgcttttgt attacacggc gaaggggtct
     4561 tgcaccaaac attgggtcga agccttcttt tgccagcaaa tccttggctt tgtcggtaac
     4621 ttcgataatt attccctgtt cggtgagtct gtcgttcacc tcatttacca tgagtgaaac
     4681 tattgaccca atgtgttctt cgttgagggc gtggaaaact atgatctcat ctatgcggtt
     4741 tagaaactcc ggcctgaaag tcctctttag ttcgcttaag accctttctt tcatatcggc
     4801 gtaatcttgc tcttcatctt ttaccggttc aaagcccagc actttttgcc tttgaatgag
     4861 atttgcgccc acgtttgaag tcattataag tatggtattc ctgaagtcga cggtccgacc
     4921 cttggcatct gttagcctgc cgtcttcgag aatctgcagg aggatgttga atacatcggg
     4981 atgggccttt tctatttcgt caaaaagcac caccgagtat ggcctgcggc gcactttttc
     5041 ggtgagctct ccaccttctt cataacctac gtatccggga ggggctccca ccattcttga
     5101 aacggcaaat ctttccatgt attcggacat gtcaagtctt atcatggcgt tttcatcacc
     5161 gaacatggca tcagccagtg cccttgcaag ttctgtcttg ccaacgccgg taggtcctaa
     5221 gaagatgaat gaaccgatgg gccttgaagg atcctttaaa cccgcatagg cacggcgaat
     5281 ggccctggat atggctacca ccgcttcttc ctggccaatg accctattgt gcaggatttt
     5341 ttccatgtta agcagccgct cggattcctc ttcagccaat cgctgaacgg gaataccggt
     5401 ccaactggcc acgatttcgg caatatcttc ttccgtcacg gtggtgttat ccagcttgtg
     5461 gtccttttgc cactcctgtt ttaattgttc aagcttggct ttggtctcct gttccttatc
     5521 cctgaggctt gccgcttttt caaattcctg ggctttaatt gccgcatctt tttccttgcg
     5581 gatgccttcc agctcttcct ccacttcttt gagctcagga ggagtagtga gggtctttag
     5641 gcgaaccctg gatgcagctt cgtcaatcag gtcgatggcc ttatccggca aaaacctgtc
     5701 ggttatatat ctgtgcgaaa gttttgctgc agcttctagg gcctcgtcgg aaatcttcac
     5761 cctgtggtga gcttcatacc tgtctctcaa gcccttgagg attgcgatgg tatcttccac
     5821 cgttggttca tcaacggtta tgggttggaa gcggcgctct aaggccgggt ctttttcgac
     5881 gtgttttctg tactcatcca gggtggtagc acctattgtc tgtatttccc ccctggccag
     5941 ggcgggtttt agaatgttgg aagcatcgat tgcgccctcg gctgcaccgg cccctatgat
     6001 cgtgtgcatc tcatcgataa agagtattac gttactcgcc tgtttgatct cattcatgac
     6061 cttttttaag cgctcttcaa attctccccg aaacttggtt cccgcaacga gggatgccat
     6121 gtccaggctt acaacccttt tatcctttaa aatctctggc acatcctgtt ccacgatttt
     6181 ttgggcaagg ccttcaacta cagccgtttt gcctacaccg ggttcgccga tgaggcaggg
     6241 gttgttcttg gtgcgccgtg agagaacctg tatgacccgc tctatttcct tttcacggcc
     6301 taccaccggg tccagcttgc cctccgcggc aagttcagtc aggtcccggc cgaactggtt
     6361 aagggttgga gtattggccg tttttttgtg gccgccctgg gccttgggat ctcccaggag
     6421 gtttaagacc tctttttgtg ccttgtccag gctcactccc aggttgttca gcacctgggc
     6481 tgcaacgcct tcaccttccc gaatcaggcc gagcagaatg tgctccgtgc ccacataatt
     6541 gtggcccagc ttcctggact catccatggc cagctccata accctttttg ccctaggtgt
     6601 ataaccgatg ggaccctgaa tcgggaatgg ccctgctcct atcatggcct caacttgact
     6661 tctgactttg cttacatcta ttccaagatt ggctaaagct ctagcagcta caccttcacc
     6721 ttctctaatc aaacccaaaa gtagatgttc ggtgcctact acattgtggt tgaggcgccg
     6781 tgcctcttcc tgggcgtagg caattacctt ttgagcacgc tgtgtaaact ttccgttcat
     6841 gttctcacct catctttgca ttttttgtct tatgaaattc gcccggtaga tgtcacgttc
     6901 caatggggtt gtagcattct cggatacttc ctgaatatat gccgggcccg tcatgatgaa
     6961 taattcgttt aaaaccgacg gtaccaaatc cttaataatg cccatatcga cacccaatct
     7021 cacatcggat ataaaagaca tggcttcctg tgatgtaaca agtttggcat ttgtcagtat
     7081 tccgtaagac ctcaagatct tgtcttcaag gcggagtctt gtttcacctg atagattctg
     7141 cctggttttg cgttcactca tcaggatttg acgcgcgaca cttacgacgt tttgtataat
     7201 atcttcttcc gattttccca gggttatctg gttggaaatc tggtataagt tgcccttggc
     7261 ttgcgagcct tcaccgtaga caccccttac tacaaggcct acctgggata tggttgacaa
     7321 gatctggcca atcttttcca tcatggtcag ggctggcaga tgcatcatta cagatgccct
     7381 catgccggtg cctacgtttg taggacatgc agtgaggtat ccgatttttt catcgaaggc
     7441 aaaatccagc ttttcttcaa gcaaatcgtc aatcttgtcg atttgcctca gtgcctcttc
     7501 cagctgcagg ccgggaagca tcacctgtat cctcaggtgg tcttcctcat ttatcattat
     7561 actcacggtt ttgtcatggt tgattatgac agcgcctctt tccgagtttg ccaaatccat
     7621 gctgataaag tgcatctccg taagcatggt cttttgttct gccgtcagtt cattcatttt
     7681 atacagctta atatccttgg aaaacacagg gttttccttt aaagccgagt acacatcatc
     7741 tactatttga ttggcttgcc tctctgaaag aagatggggg aaggggacct cgtttaagtt
     7801 gcgtgccagt ctcaccctgc tgcttataac tatatcgctt tccgggccag gagaattgat
     7861 ccattttatc cgtgacgtat ccatcaaatc ttccaagctc atccgatcat ctcctaactg
     7921 cctatttggt tctgttccag ttcttttatc ttatccctta taacggctgc ccgttcgtat
     7981 tcttctgcat ttacggcttc ctgcaattcc cgctttaatc tgtcgatttc ctgctttatt
     8041 ctaatttgac ctccggacct ctttgggact ttccctacat gaaaggtctt cccgtgaatc
     8101 ctccgaagca tagggttcat ctgattctta aaggtgctgt aacactgact gcagccaaag
     8161 cggcctgtct ttgaaaaagt ttgaaaagtc agtccgcacc tgccgcactt gaattcttcc
     8221 gagctgccga tgggagtttt gctgctaggt tcaaaaaagc tagacagcaa tttggccacg
     8281 gaaaagcccg tatcttcaaa gccaaatccg cctatacttg aagataatga aaggtgcttt
     8341 tcctttgcac actcttcaca tagatgcatt tcggtttttt cattgttaac tatcttagtt
     8401 atatgaactg tagccggtct catatggcac tcttcgcata acatggttat gctccccctt
     8461 tcctagcttc aaagtccagc aaggctgtaa tcacagcctt catgatgcgt gcccggacca
     8521 tgtcgcggtc ctgagggcac agcggcaaat tgtatttgtt tatagcagca tttaaaattt
     8581 tcatttccct ttccgttatt acatcttcct cccacaatct ctcaagaata gctttgctgt
     8641 cggaaccgga aatggaatcg ccgatatact cgataatgtc ctttaaaaac tcatcttcat
     8701 ttatttttag cttttttatc ctaatatatc ctccgcctcc ccggcggctt tcgacataat
     8761 agccccgttc caaagtaaag cgcgtggtaa ggacgtagtt tatctgggaa ggggcacatt
     8821 gaaagtagtt tgccatttca ttgcgctgta tttcaagaat cccttccggc atgtcctcaa
     8881 acattctctt gatgaaattt tcgatcatat ccgaaaggtt tgccatgcaa ttctcctccc
     8941 attgagcttg tattgggtta tttgactttc cctgaccttt acttatatta tattttaaac
     9001 taacaaaaaa tgcaagtgtg tttatagaaa ataaagggag gacaaagtcc tcccttcgct
     9061 atcgcttctt ttttcgcttg gaagttgaaa gttcttcaga gatctcaaca agacgactgg
     9121 ccctttgttc ctgatcttca gaagaagtcc aacccataag gcttggaatt atttcgccat
     9181 acattgtgtc atccagcagc tggtcctcaa tcaggctatc aaccttcttc ttgtgaatgt
     9241 cctccactct gtcaggcgtt ggacctacat gcatcttttt tttagacctt accatggcgt
     9301 ccctcctttt ttctgcttat cagcctacat ctaagcattt ccttaaatgc gaccataaat
     9361 gcaggaaagg agggagcata ttatggtaaa attactcctt gtttttttga gcttcagcta
     9421 ttactttttc ggcaatgggt gcaggaacct cctcgtagtg actgaaactt gaggagaaag
     9481 agcctctgcc ttgggtcatg gaacgcaggt ccgtagcgta gcggaacatt tccgccatgg
     9541 gcacctgagc cttgatgtgc tgcatgcctg cttttggctc catgccgagg actctgccgc
     9601 ggcgcttgtt caggtcgccg ataatatcgc ccatgtaatt gtcgggaaca actacggtta
     9661 cgtccatgat cggttccaag agcaccggct ttgcctcagc tgcgcccttc ttaaatgcca
     9721 tggagccggc tattttaaag gccatttcag aagagtccac tgggtgatag gaaccatcgt
     9781 acaggatgac ctttacccct accatcggat agccggccaa gacgccgtcc tgtagggctt
     9841 ccctgatacc tttttcaacg gcagggacat actgtttggg cacaactccg ccgaatatct
     9901 tatcttcaaa ttcaaactca ttttcaaggt ccaggggctg gatttctatc caaacgtgac
     9961 cgtactggcc acggccaccg ctttgtttct tgtgcttgcc ttcaacttta gtggttccgc
    10021 ggattgtttc acgatagggc actgccgggg tatccagtac catttcggaa ccaaatttat
    10081 ttgcaagtct cgcacccaag acttccaggt gaatctcacc catacccgat accaacagct
    10141 gaccggtttc gttgttctta gtgacttcaa aggtcttgtc ttcctccatc agcctttgaa
    10201 gacctgaaga aatcttgtcc tcgtcgcctg aggacttggg cgctgcggca agggtaagta
    10261 caggtttcgg gaaatcaatg ggcttgagta cgacgggatt gtctttttct ccaagggtgt
    10321 catttgtggt ggtatgctgc agttttgcca ctgcggctat atcccctgca aatacttccg
    10381 tgatattctc ctgtttcttg cccctcataa catagatctg accgaatttt tcggttttgt
    10441 cctgtgtcac attgtacacc gaactgtcgg actttaagct acctgaaaac accttgaaca
    10501 gtgtgagcct tcccacatag gggtcagcca tcgtcttaaa caccagggcc gagaagggtt
    10561 catcggctct acattttctt ataacttctt catcggagcc gg
//
LOCUS       DAONUO010000026        10408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_4922_length_10408_cov_3.738723, whole genome shotgun sequence.
ACCESSION   DAONUO010000026 DAONUO010000000
VERSION     DAONUO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4922_length_10408_cov_3.738723"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PK768_01670"
     CDS             complement(<1..243)
                     /locus_tag="PK768_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HQA59709.1"
                     /translation="MLTDVKGILVGHAQDNEAGTGCTVVLCKDGAVAGVDVRGGAPGT
                     RETDLLEPENMIQKVHAIYLGGGSAFGLDGVSGVMRF"
     gene            complement(263..1306)
                     /locus_tag="PK768_01675"
     CDS             complement(263..1306)
                     /locus_tag="PK768_01675"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HQA59710.1"
                     /translation="MGKIRVLVVDDSAFMRTFISDIISKEPDMLVIDTAGDGDAAISK
                     VKAMNPDVVTLDVEMPGKNGLEVLKEIKNISSAQVIMLSGLTTEGSATTIEALRIGAF
                     DFVAKPGDTSFNRITDMKRDLLSKIRQAFVAKNKTMRKIFRKDKQDHKIQKKAKGKIE
                     AVVIGASTGGPKVLYDIITRLPADMLVPVFVVQHMPQGFTKAFAERLDKQANLKVVEA
                     THEESIRPGVVYIAPGGYHMLLTENKIILDTSPPIHGVRPAVDKLFISAAKVFEEKTL
                     ACVLTGMGRDGAEGVRAVKAKGGFTIAQDEATSVIYGMPKAAYETGCIDMVLPDNDIL
                     EEIIRLVKTNDKN"
     gene            complement(1331..2665)
                     /locus_tag="PK768_01680"
     CDS             complement(1331..2665)
                     /locus_tag="PK768_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQA59711.1"
                     /translation="MGNQTSIFDEEAKISIPLADRMRPRNLDEFVGQEHILGDGKILR
                     KLIESDSITSMILWGPPGVGKTTLAKIIAKRTKAKFENFSAVLSGIKEIREVMKQAEK
                     RRLYGQRTLLFIDEIHRFNKSQQDAFLPFVERGDIILVGATTENPSFELNSALLSRSK
                     VFLLNPLKPEDIVVLLKKALGDEKRGLGSMKISVADDILEKISLYADGDARVALNTLE
                     LAVLSATPSSDGVIDITDKVLQEVFQKKTLLYDKMGEEHYNLISAFHKSIRNSDSDAA
                     VYWLVRMLESGEDPLYIARRMIRIASEDVGLADPRAIEQAVAAFHSAHFIGMPECSTS
                     LAQAAVYLALAPKSNAVYKAYMEAREDALKTLAEPVPMHLRNAPTKLMKELGYDEGYK
                     YAHDFENKVAAMQCLPDSLAGKDYYRPTKEGEENLYRKRKNQLKLLQEKNKI"
     gene            complement(2670..2945)
                     /locus_tag="PK768_01685"
     CDS             complement(2670..2945)
                     /locus_tag="PK768_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQA59712.1"
                     /translation="MKKSACIHLYGIVQGVGMRYQVFRKASSLGLFGYVRNMSDGSVM
                     IEVEGEEDSILFLVDYVKNDVRWAQVDDIVIKWGDHKNSYKRFEILG"
     gene            complement(3162..4640)
                     /gene="spoIVA"
                     /locus_tag="PK768_01690"
     CDS             complement(3162..4640)
                     /gene="spoIVA"
                     /locus_tag="PK768_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HQA59713.1"
                     /translation="MKKFDLFNDIVERTGGDIYIGVVGPVRSGKSTFVKKFMELMVIP
                     NISDANYRSRAKDELPQSGAGKTITTAEPKFIPSEAVEITFKDNIKFRVRLVDNVGYT
                     VRGALGYEDEDGPRMVNTPWFEEEIPFQEAAEIGTRKVIEEHSTIGLVITTDGSVTEI
                     PRENYVEAEKRIVQELKSIGKPFVIVLNSIHPSTDEVAELKEQLEVEYDVPVIPLDCL
                     QTSVDDIYMILEQILYEFPIMEINVRIPGWIQALQSEHWFRKQFEDSVRESMKDVKRL
                     RDMENTIDIFKSYDFIHEVTLKEMNLGSGTAEIILDAKKELFFKVLSEISELEIKSER
                     ELLEHIFELASAKKAYDKVAKALEDVHRVGYGIVPPQLDEMTLEEPEIIRRGSKFGVK
                     LKAIAPSIHMIKTNIQAEVSPIIGTEKQSEELINYLMGEFESDPSKLWETNLFGKSLN
                     DLVREGIQNKLINMPESAQDKIQETLQRIVNEGSGGLICIIL"
     gene            complement(4837..5871)
                     /locus_tag="PK768_01695"
     CDS             complement(4837..5871)
                     /locus_tag="PK768_01695"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQA59714.1"
                     /translation="MLKVAVIGARSWGTALAHVAAENNNNTYLWVRRRELAEEIIKTG
                     ENRPYLPGADISPKINVSTDIKQVVTDADIVIMAVPSQAIRSMARQIRSFVKEDAIIT
                     SASKGIEINSLKRMSQVLAEELDNRKSHNIVALSGPSHAEEVIRNLPTAIVAASPGQE
                     AAEKVQDVLINNNFRVYTNTDIIGVELGGALKNVIAICSGISDGLGFGDNTRAALMTR
                     GIVEITRLGVKLGASPETFSGLSGIGDLIVTCSSNLSRNRRAGIDIGRGKTVEEVMQS
                     TNMVIEGINTTRAAFMLSQKHKIEMPITEQAYRVLFKGKDPLLAVNSLMSREGKHEHE
                     DIISQRFFVY"
     gene            complement(5874..7193)
                     /gene="der"
                     /locus_tag="PK768_01700"
     CDS             complement(5874..7193)
                     /gene="der"
                     /locus_tag="PK768_01700"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQA59715.1"
                     /translation="MSFIVAIVGRPNVGKSTLFNRIMGRRISIVHDKPGVTRDRIYGE
                     TDWNGKHFTLIDTGGIEPSSKDVILKQMKRQADFAIDTADLILFMVDGKEGMTPADMD
                     VAELLRRSGKPVLLVVNKVDNYENSFDNYEFYQLGFEDPIFISASHGLAIGDLLDRIT
                     DYIEKRGEDEEDEDVTKVAIIGRPNVGKSSLLNAIIGEERVIVSDIPGTTRDAIDTYL
                     QLDGKKLIFIDTAGLRRKSRINEDIEYYSSLRAIGAINRADVVLMVLDASEDITEQDK
                     RVAGMAHDAGKAIIITVNKWDLVDKDSHTVEEFNERIRTEFAFMQYAPIVYVSAKTGQ
                     RINRIIKLINSVMENYTFRVKTSVLNSLINEATAISEPPTIKGRKLKVYYAVQTAIKP
                     PSFVFFVNDNKLFHFSYVRYLENTLRQAFGFEGTPIWIKSRQRKGSD"
     gene            complement(7285..7761)
                     /locus_tag="PK768_01705"
     CDS             complement(7285..7761)
                     /locus_tag="PK768_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HQA59716.1"
                     /translation="MKIFYCCYGSAHSSVVAASIHLGLLPSDRVPTSREFLVLPHYDK
                     TEPFEIGTPFFMGKDEYGSEIYILGMAGERPLIKRALLSFFSLLGVDTHGIILIDTLN
                     SVNLLTKIGGFFSRRLGFVTVGRPLTILGIQVKYRDFVELVKAVKKREQNKLSQLD"
     gene            complement(7758..8225)
                     /locus_tag="PK768_01710"
     CDS             complement(7758..8225)
                     /locus_tag="PK768_01710"
                     /inference="COORDINATES: protein motif:HMM:NF022827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HQA59717.1"
                     /translation="MKVIYSCYWGSCLAMVAAFLHLEYIDEEREIFPLNLFNNISDCE
                     FGELIHVGRDDKGRDVFAIGTKNSGKILAKTLVGIAGVYGFPADSVQFVDLNHLYNGT
                     ILLGVFLIRIFNLGFLGRRLIVSGIKKNFGRLKNLVQSVRAKQLAPTERGGSR"
     assembly_gap    8352..8361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8362..9468)
                     /locus_tag="PK768_01715"
     CDS             complement(<8362..9468)
                     /locus_tag="PK768_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HQA59718.1"
                     /translation="MKKGNSGKKIITVIVIVIILSIFCSTALAEEERDEGYYTVYEEG
                     TNKKIFSTARVLHIGDQYLNEDNILYEITKISGDKAYAKFKERVDIQKTLGQTESQDE
                     ALVFGDFSLEVADTKKEKVIAIYHTHSDESYIPTDGKASIPHNGGIYKVGKALKAALE
                     EKGIKVIQSEKSHDPHDSMAYQRSRRTAAELLKNAPDAIVDIHRDAVPAEEYYGNVNG
                     QHLARIQLVVGRQNPQMETVNNFAKQLKATADNKYPGLIKGIFYGKGAYNQDLYPRSI
                     LVEAGTYTNSRYKAQDGANIMADVIATTIYGEDYAKESTPGKGATTKIRGEGGGSSRA
                     LLWILGIAVLGFGAYMLISTGGINELSEKVKRFSS"
     gene            complement(9452..10147)
                     /locus_tag="PK768_01720"
     CDS             complement(9452..10147)
                     /locus_tag="PK768_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HQA59719.1"
                     /translation="MPAGVILLLVIAVLIYFGLLQRVLDRMHMTDSMAIIFIGLMVIG
                     SFLPDIPLGGRMSINIGGGIVPIALALYLIVTADSNQEKVRSIIAALITGTAIYVAGR
                     ILPAEPGTMIVEPILAFAIIAGIIAFIFGRSRRGAFIAGILGIIISDIISALGIAARP
                     VGTTIGGAGILDAAVISGVIAVGLCEIVGETGEKLLGGSWAKKKAEKSSVTSSLVERK
                     SPQEEGEDCEEGE"
     gene            complement(10218..>10408)
                     /locus_tag="PK768_01725"
     CDS             complement(10218..>10408)
                     /locus_tag="PK768_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HQA59720.1"
                     /translation="TGKGKWKLDLVLTNRKQLEEVGINPQNITESGFCTSCRNDIFFS
                     HRKDKGKTGSLAAVLQLI"
BASE COUNT         2711 a         2501 c         1781 g         3405 t
ORIGIN      
        1 aaaacgcatt acccccgaga caccgtctaa gccaaaggca cttccaccgc ccaagtaaat
       61 agcgtgaact ttttgtatca tattctcagg ttccaataag tcggtttcgc gggttcccgg
      121 agctcccccg cggacatcta cacctgccac tgcaccatct ttacataaga cgaccgtgca
      181 gccagtacct gcctcattat cttgggcatg accaacgaga attcccttta catctgtcag
      241 catattgtct tcctctctca acttagttct tgtcgtttgt tttaacgagt cttattattt
      301 cctctaaaat atcattgtca ggaagcacca tatcaataca ccctgtttca taagcagctt
      361 taggcatccc gtagatgacg gatgtggctt catcctgagc aatagtaaaa ccgcccttag
      421 ctttaacggc cctaacgcct tccgctccat ctcttcccat ccctgtaagc acgcaggcca
      481 gtgtcttctc ttcaaagact tttgcagctg aaataaacaa tttatcaact gcaggtctca
      541 ctccatgaat aggaggagat gtatcaagta ttattttgtt ttcagtaagg agcatatgat
      601 atccacccgg tgcaatgtat acaactccgg gtctaattga ctcttcgtgt gtagcttcaa
      661 caactttgag gttcgcctgt ttatcaagcc tttctgcaaa ggctttagtg aagccctgtg
      721 gcatgtgctg aacgacaaat actggcacaa gcatgtcggc tggtaaccta gttataatat
      781 cgtataaaac tttcggtcct cccgttgaag cacctataac taccgcttct attttgccct
      841 tagctttttt ttgtatttta tgatcctgct tgtcttttct aaaaattttt ctcattgtct
      901 tgttctttgc tacgaatgct tgtcgtattt tgctaagcag atccctcttc atgtcagtta
      961 ttcgattgaa tgatgtgtca cctggttttg ctacaaaatc aaaagctcct attctcaatg
     1021 cttctatggt tgtggcagaa ccttctgtcg ttaaaccgct taacatgatt acctgagcag
     1081 aacttatatt ttttatctcc ttcaaaactt caaggccatt ttttcccggc atctccacat
     1141 ctagtgtaac cacatccgga ttcattgcct ttacttttga tatagcagca tctccatctc
     1201 cggccgtatc aattactagc atatccggtt ccttacttat tatgtccgaa atgaatgtcc
     1261 tcataaaagc ggaatcatca accaccaaca ctcgaatttt ccccatcata tctgcctccg
     1321 aaaatagtat ctaaattttg tttttttctt gcaacaattt aagctgattt tttctttttc
     1381 tataaaggtt ttcttcacct tcttttgttg gacggtagta atctttgcct gctaaactgt
     1441 caggtaggca ctgcatggca gccactttgt tttcaaaatc atgggcatac ttatagccct
     1501 catcgtagcc caattccttc attaactttg tcggagcatt tctcaaatgc atcggaactg
     1561 gctcagctaa agttttcagt gcatcttctc ttgcttccat ataagccttg tatacagcat
     1621 ttgatttcgg tgccagggca agataaactg ccgcttgggc aagacttgta ctacattctg
     1681 gcatgccaat aaaatgggct gaatgaaaag ctgccacagc ttgttcaata gctctagggt
     1741 ctgcaagccc aacatcttct gatgcaatgc gaatcatgcg cctggctata tacaatgggt
     1801 cttcaccgga ttctagcata cgtaccagcc agtaaacagc agcatcactg tcgctgtttc
     1861 tgatggattt atggaaggct gatattaaat tgtaatgttc ctcgcccatt ttatcgtata
     1921 agagggtctt tttctgaaag acttcctgca gaactttatc cgtaatatca attactccat
     1981 ctgaagaagg tgtagcgctc agcaccgcta attccagggt attaagggct actcttgcat
     2041 caccatctgc atatagggaa attttttcta atatatcatc tgcgacactt atcttcatac
     2101 tgcctaaccc acgcttttca tcccccaagg ccttttttag caatactact atatcctcgg
     2161 gttttaatgg attcaataag aacactttcg aacgtgaaag aagagcagaa tttaattcaa
     2221 aggaaggatt ctccgtagta gcacctacga gaattatatc ccctcgttct acaaagggaa
     2281 gaaaggcgtc ctgttgtgat ttgttaaacc tgtgaatctc atcaataaaa aggagtgtcc
     2341 tttgtccgta cagccgacgc ttctcggcct gcttcatgac ttccctgatt tctttaattc
     2401 ctgaaaggac agcgctgaag ttttcaaatt tggctttggt tctctttgca attatctttg
     2461 ccagagttgt tttcccaact cccggcggtc cccacaatat catagaggtt atgctatcac
     2521 tttcgatgag ttttctcaaa atcttgccat cccccagaat atgctcctgc cccacaaact
     2581 catctaagtt tctcggtctc atccggtctg ctaggggaat cgagattttt gcttcttcat
     2641 caaatataga tgtttgattg cccattttat caccctaata tttcaaaccg cttatagctg
     2701 tttttatgat ctccccattt tataacaata tcatctacct gagcccaacg cacatcattt
     2761 tttacataat caacaaggaa aagtatggaa tcttcctctc cctccacttc aatcataaca
     2821 cttccatctg acatattcct tacataccca aacaagccta gacttgaagc ttttctaaat
     2881 acttgatatc tcatacctac gccttggacg atgccgtaaa ggtggatgca agcagatttt
     2941 ttcattaaaa acctccttac actcgttgca taatcattgg tattttctca atataacatc
     3001 attgatagtt ggaagcagaa ctcaatctta gctgtattat accatttaaa attagttttg
     3061 ttctatatta tcataaaaaa tctgccgcaa aatttgacct acagtgaaat aaaataagtt
     3121 aagctaatgg tgctagcacc ttaacttaac ttgtttgagc attacagtat gatacagatt
     3181 aggccgccac tgccttcatt gactatgcgt tgcagggtct cctgaatctt atcttgggca
     3241 gattcaggca tatttatcag cttgttttgg atgccttctc tcacaaggtc atttaatgat
     3301 ttcccaaata gattggtttc ccacagtttt gatggatcgc tttcaaactc acccataaga
     3361 taattaatta gctcttcgct ctgcttttca gtaccgataa tgggagatac ttctgcctgg
     3421 atgttggttt ttatcatgtg aatagaagga gctatagctt taagctttac tccaaatttc
     3481 gaaccccgac gaataatctc tggttcttcc agtgtcattt catcaagctg aggcggaact
     3541 attccgtaac ccactctatg aacatcttct aatgccttcg ctactttatc ataggctttc
     3601 ttcgcactgg caagttcaaa gatatgttct aaaagttctc gttctgattt tatctcaagt
     3661 tccgatattt cacttaaaac cttgaagaac aactcttttt ttgcatccaa aataatttct
     3721 gcagtgcctg aacctagatt catctctttc aatgtaacct catgtataaa atcgtagctt
     3781 ttaaatatgt ctattgtgtt ttccatgtcc cttaaccttt ttacatcttt catgctttct
     3841 ctaaccgagt cttcaaattg cttcctaaac caatgctcac tctgaagtgc ttgtatccag
     3901 cccggtatcc gaacattgat ttccatgatt ggaaactcgt ataatatttg ttccaatatc
     3961 atgtatatat catcaacact cgtttggagg caatccaagg gaatcacggg cacatcgtat
     4021 tcaacctcaa gctgctcttt taattctgca acttcatcag tggatggatg tatagagttc
     4081 aacacgatta caaaaggctt tcctatggat ttgagttcct gtacaatcct tttttctgct
     4141 tcaacgtaat tttctctcgg gatttcggta accgaaccat cagtggttat aactaaacca
     4201 atagtggaat gctcttctat aacctttctt gttccaattt cggcagcctc ttggaaggga
     4261 atctcttctt cgaaccaggg tgtgtttacc atcctgggtc catcttcatc ttcatagccc
     4321 aaagctcccc ttaccgtata gcccacatta tctaccaatc tcaccctaaa tttgatgttg
     4381 tctttaaagg tgatttcaac cgcttcactg ggaatgaatt tcggttcagc tgttgtgatg
     4441 gttttgccgg ctccgctctg gggcaactca tcctttgccc ttgagcgata gtttgcatcg
     4501 gatatgttag gtatgaccat tagctccatg aactttttca caaaagttga tttccctgat
     4561 cttacgggac ctacaacccc aatataaata tcccctccgg tcctttcaac aatatcatta
     4621 aataagtcga attttttcat ccatttccct ccctttttgc tcttcccacc ccgtttcgtg
     4681 tcaacaaagg ctcgattatt tctgtcaatg cttatccgaa ttttctgtgg aggggtggaa
     4741 tactttgctc ctatacaata tatatatgac aagggtatat ctttattact aaaattaaaa
     4801 aaaacgtggc cttgatatgt atcaattggc cacttattaa tatacaaaaa atctctgtga
     4861 gattatatct tcatgttcat gtttgccttc cctgctcatt agcgaattta ctgccagcaa
     4921 tgggtctttt cctttaaaaa gaacccgata tgcctgttcg gtaattggca tttcaatttt
     4981 gtgcttctgt gacagcatga aggctgccct agtggtgttt atgccttcaa tgaccatatt
     5041 ggtagattgc atgacttctt ctactgtttt tccacgccca atgtcaatgc cagcccttcg
     5101 gtttctgctt aaattactgc tgcaggtgac tattaggtcg ccaattcccg ataaacctga
     5161 aaatgtttcg ggacttgcgc ctagtttgac cccaagacgt gttatttcaa ctattcctcg
     5221 agtcatcaaa gctgccctag tgttgtcccc gaatcctaga ccatcggaaa taccggagca
     5281 gattgcaatt acgtttttca gagcccctcc aagttcaact ccaatgatgt cggtgttggt
     5341 gtatactcta aaattgttgt taatgagcac gtcctgaacc ttttctgctg cttcctgccc
     5401 gggagaggca gccacaatag ctgttggcaa attcctgatg acctcttcag catggcttgg
     5461 gccagatagg gctacaatgt tgtgactttt tctattatca agttcttccg ccaaaacttg
     5521 tgacatgcgt ttgagactgt ttatctcgat accctttgat gcacttgtaa taatagcatc
     5581 ttcttttacg aaactgcgaa tttgccttgc catgcttctg atcgcctgag atggcaccgc
     5641 cattatcact atatccgcgt cggtcactac ttgtttaatg tcggtagata catttatctt
     5701 cggggaaata tcggcaccgg gcaaataagg cctgttttct ccagtcttta ttatttcttc
     5761 tgccaactcc cgccttctga cccacaagta cgtattgttg ttgttttcag ctgcaacatg
     5821 tgcaagcgcc gtgccccaac ttctagctcc tatgactgct acttttagca cacttaatcg
     5881 ctccctttcc tttgacgaga ttttatccat ataggagttc cttcaaatcc aaaggcctgt
     5941 ctgagtgtat tctctaaata tctaacgtac gaaaaatgaa aaagcttgtt atcattgaca
     6001 aaaaaaacaa aagacggtgg ctttatagca gtctgaacgg cataatatac cttcaatttc
     6061 ctacctttta tcgttggcgg ttctgatata gccgtagcct cattaattaa actattaagt
     6121 acacttgtct tgactctgaa agtatagttt tccattacag aattgatgag tttgattatg
     6181 cgattaattc tttgaccggt cttagctgat acatagacaa tcggcgcata ctgcataaaa
     6241 gcaaattcag ttctgattct ttcattgaat tcctctacag tatgtgagtc tttgtctacc
     6301 aaatcccatt tgtttacggt gatgattata gctttgccgg catcatgagc catgccagca
     6361 acacgcttgt cctgctccgt tatgtcctct gatgcatcca gaaccattag caccacgtcg
     6421 gcccgattga tagctcctat ggcgcgcaga ctgctataat attctatatc ttcgttgatt
     6481 ctgctttttc ttctaagacc cgcagtatcg ataaaaatca atttcttccc gtctaattga
     6541 aggtatgtat ctattgcatc tcgagtggtt ccgggaatat cgctgacaat aaccctttct
     6601 tctccaatga ttgcatttag cagcgatgat ttccccacat tgggtctgcc aataatggcc
     6661 actttggtga cgtcttcatc ttcttcatct tcaccgcgtt tttcgatata atcagttatt
     6721 ctgtcaagca aatctccgat agccaaacca tggcttgctg atataaatat agggtcttca
     6781 aagcccaact gataaaactc gtagttatca aagctattct cgtaattgtc cactttgttt
     6841 acaaccaata acaccggttt tccgcttcta cgcaaaagct cggccacatc catatcggca
     6901 ggcgtcattc cctcttttcc atcaaccatg aacaaaatta agtctgcggt atctatggca
     6961 aaatcggctt gccgtttcat ctgctttaat atgacatctt tagatgatgg ttctatgcct
     7021 cccgtatcaa tcaaggtgaa atgtttgcca ttccaatccg tctcaccgta tatcctgtca
     7081 cgggttacac cgggtttatc atgaacaatg gaaatccttc tgcccataat tctgttaaac
     7141 agtgttgact tgcccacatt gggcctaccg acaatagcta ctataaaact catctttctt
     7201 ctgcccccaa ttcttttatt caggaaatgc atttgtaatt taactattat tacctgcatc
     7261 aatattattg tcaataaata gcctttagtc aagttgactt agcttgtttt gctccctttt
     7321 ttttacagct ttaacgagct caacaaaatc cctgtatttt acctgtatgc ccaaaatggt
     7381 aaggggccgc cctactgtta cgaaacccaa tcttcttgaa aaaaaacctc ctattttggt
     7441 taagagattt acactgttta gggtatcgat aaggattata ccatgagtat cgacgcctag
     7501 aagactaaaa aaacttaaca atgccctttt tatcaatggc cgctcacccg ccatgcccaa
     7561 aatataaatt tctgagccat actcatcctt acccataaaa aagggtgttc ctatttcaaa
     7621 aggctccgtc ttgtcataat ggggcagcac taaaaactct cttgatgttg gcactcgatc
     7681 cgaaggtaat aaaccgagat gaatggaggc ggcaactacc gaagaatggg cacttccata
     7741 acagcagtag aatattttca tcgactcccc cctctttctg taggagccaa ttgcttagcc
     7801 cttacacttt gaaccaagtt ttttaatctg ccaaagtttt ttttaatccc cgaaactatt
     7861 agtcttctac ccaagaaacc caaattaaat attctaatca aaaaaacacc taacagaata
     7921 gtgccattat aaagatggtt taggtctaca aattgcactg agtcagccgg aaaaccataa
     7981 acccctgcga tgccgactaa ggttttagca agtatctttc cactgttttt tgttcctatt
     8041 gcgaatacat ctcttccttt atcatccctt cccacatgta tcagttcccc aaattcgcaa
     8101 tcactaatat tattgaacaa gttcagggga aaaatctctc tttcttcgtc aatgtactca
     8161 agatgcaaga aagcagccac catagcaagg cagctgcccc aataacacga ataaattact
     8221 ttcatacaaa ctcccccggt gtcctacatt gataaacagt tttagttttt ttgttcatca
     8281 tcatcttcac tttcagaccc gcattcccct gaggcagatt ttttgtcacc taaaaaattc
     8341 gcaaattctc tnnnnnnnnn ngcttgaaaa tcgttttact ttctcgctca gttcgttgat
     8401 gcctccagtg gatattagca tataagctcc aaagccaagc actgctattc ccaaaatcca
     8461 cagcaaagct cttgagctgc cgccaccttc tccgcgtatt tttgtagttg ctcctttacc
     8521 gggtgtactc tcctttgcat agtcttcacc gtaaatggta gtggctatca catctgccat
     8581 tatattggca ccgtcttgcg ccttatatct tgaatttgta tatgtgcccg cttcaaccaa
     8641 aatacttctc ggatataaat cttggttata tgcgcctttg ccgtagaaaa ttccctttat
     8701 tagaccgggg tatttgttgt cagctgtggc ctttagctgc ttggcaaaat tattaaccgt
     8761 ttccatttga gggttttggc gtcctacgac cagttgtatc cttgcaaggt gttgaccatt
     8821 aacattccca tagtattcct ctgctgggac tgcatctcga tggatatcta caatggcatc
     8881 cggagcattt tttaaaagct cggcggcggt gcgtctggac ctttggtagg ccattgaatc
     8941 atgagggtcg tgagattttt cagattggat taccttgata cctttttctt ctaaagctgc
     9001 ctttaatgcc ttgccaactt tatatattcc gccattgtga ggaatgctgg ctttgccgtc
     9061 agttggaatg taggattcat cgctgtgagt gtgataaata gcaattactt tttctttctt
     9121 ggtgtcggct acttccaaag aaaagtctcc aaagactagt gcctcgtcct gggattcagt
     9181 ttgccctaag gtcttttgta tatccaccct ttctttaaat tttgcatagg ccttatcccc
     9241 agaaatcttt gttatttcgt ataaaatatt atcttcattt aggtactggt cccctatatg
     9301 caatactctg gcagtggaaa aaattttctt gttggttcct tcctcataaa cagtataata
     9361 accttcatct ctctcttcct cagctaaagc cgtggagcaa aaaatggaga gaataattac
     9421 aatcacaata acagttatta tttttttgcc gctattcccc ttcttcacag tcttcacctt
     9481 cttcctgggg actcttccgt tctaccaagc tgctggtcac cgaggacttt tcggcttttt
     9541 tcttcgccca gcttccaccc aagagttttt ctcctgtttc gccgactatt tcacacaatc
     9601 caactgcaat aacccctgat atgacagctg catcaagaat tccagccccc cctatagttg
     9661 tccccacagg ccttgctgct atgcccaggg cagaaataat atcgcttatt attataccca
     9721 gtatgcctgc aatgaaagct ccccttctag aacggccaaa aataaaggca attattcccg
     9781 cgatgattgc aaaagctaat atcggttcaa caatcatggt cccgggctct gccggcaata
     9841 tcctgcccgc aacataaatt gcagttcccg ttatcaaagc tgcaattatt gatctcactt
     9901 tttcttgatt cgaatccgca gtaacaatta gataaagggc taaagcaatt ggcacaatcc
     9961 ctcctcctat attgatggac attcgtcctc caagagggat atcaggcaaa aaactgccga
    10021 taaccattaa accgataaaa attatcgcca tgctgtcggt catgtgcatt ctgtctaaaa
    10081 ccctttgcag taaaccgaag taaatcagaa ctgcaattac cagcaataaa ataacccctg
    10141 caggcatcgg gtattctcct ttcctggaaa atgtctgtaa ttatgatgcc ctcaggagta
    10201 aaaaaatata cttgcgacta tatgagctgc agaactgctg ctaggcttcc ggttttacct
    10261 ttgtccttcc tgtgtgaaaa gaagatgtca ttcctacaag atgtgcaaaa gccgctttcg
    10321 gttatgtttt gcggattaat tcctacttct tctaattgct ttctgttggt taaaacaagg
    10381 tccagcttcc acttgccttt acctgtct
//
LOCUS       DAONUO010000027        10242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5022_length_10242_cov_4.157357, whole genome shotgun sequence.
ACCESSION   DAONUO010000027 DAONUO010000000
VERSION     DAONUO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5022_length_10242_cov_4.157357"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..780
                     /locus_tag="PK768_01730"
     CDS             97..780
                     /locus_tag="PK768_01730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQA59721.1"
                     /translation="MYYYEEASEKNWFRPENLKRITICIIFIIVVLLLKKVNMPIFKT
                     ALSKIEYYLCDYSYEYQDIVEAVKNISEAPDKIPVLSQGSSKLLPMPVHGSISSEYGL
                     RLHPILKQQQMHKGIDIVEKEGAPVKVVIDGVVDSVGYDEELGNTVKISHGNGLTTVY
                     AHLKDVYVKEKETVKQGFIIGTIGNTGLSETAHLHFEIWKDGKAEDPRKWLNIQDNTR
                     EDLDGFKGL"
     gene            761..1624
                     /locus_tag="PK768_01735"
     CDS             761..1624
                     /locus_tag="PK768_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HQA59722.1"
                     /translation="MVLRVSNCKVSISSLFIAAIVLALAAGMHIEMLAALFALGVHEF
                     AHVFAGSRLGIPIHEIEILPFGGRIISGISDATDESEVIMVLAGPLANFAAAGFMLGL
                     SYFAILPAKIAWQFINYQLMLGVFNMLPAFPLDGGRIFALWLSKHMTFTSSVRIASHA
                     GKILAYSLLLTAFIGIIFKKINFSLIITGFFLLLQASKEEENAHLIFMNYLAKKKEKL
                     LKNQYFPGEIVVVDQEATAKKILYLFLPKKYFIVYILDRNMEIRKTLTETEIFDIIME
                     KGLDFKMKELI"
     gene            1676..3529
                     /locus_tag="PK768_01740"
     CDS             1676..3529
                     /locus_tag="PK768_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HQA59723.1"
                     /translation="MTIENQLEKLLSRVSKPTRYIGNEYNSIRKDLDNIRVHIALAFP
                     DVYEVGMSHLGIKILYHLLNEYPDIYAERVYAPWVDFEDLMRKEGVPLFSLESKTSIS
                     QFDFLGFTLQYELSYTNIVNMLELSNVPIFSRERNDSHPFVIAGGPCAFNPEPLADII
                     DFFVIGEAEEAILEVIDLYKDFMERGESRQKFLCNVARIPGVYVPSLYSASYNSDGTI
                     SSIVPIKDHIPKRVQKRVIQNLDNVYYPTKFIVPYMDIVHDRASLEIFRGCTRGCRFC
                     QAGMIYRPVRERSVGRLEKLAKDIISSTGYGEISLLSLSSSDYSDLKRLAELLTESFR
                     ESKVGLSLPSLRIDAFSLGLAEKVQEIRKSGLTFAPEAGTQRLRDVINKGVTEEDLLS
                     SVRDAFALGWNKVKLYFMIGLPTETDEDIKGISELAHAVVDVYRQVNGHTKGLTVTVS
                     TSTFVPKPFTPFQWESQITLEEIYERQNLLKKLLRKNRNISYRWHDGKLSFLEAVFSR
                     GDRRLGNVLKIAHEKGCKFDSWDEFFNFDKWMSAFEEAEIDPDFYAYRKRSESEKFPW
                     EIIDAGIDRKFLLRELERAKRGQITPDCRISCYGCGIKRLGDGFCEAKIEV"
     gene            3498..4181
                     /locus_tag="PK768_01745"
     CDS             3498..4181
                     /locus_tag="PK768_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HQA59724.1"
                     /translation="MVSVKLRLRFKKEGLARFISHLDLMRTFERAFRRAELPIAFSQG
                     FNPRPKMTFASALSVGISSSSEYLDVEFSEDVSASDVALKLNPALPEGIKVTDVKMAD
                     EAISLSMLNGAKYVVRLALNEVDLEELKKSTKGLLEQKEIIIEKRTKSGKKLVNIAPF
                     IYSLGLSNCDGSAADIVMSVAIGQQGSVAPTMVINELERILCKELEIVSIHREAIFYK
                     SNGKEILPM"
     gene            4234..5925
                     /locus_tag="PK768_01750"
     CDS             4234..5925
                     /locus_tag="PK768_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQA59725.1"
                     /translation="MSKEILADVDRDQIRIGVLEDRQLVEYYVERTHDERVIGNIYKG
                     KVANVLPGIQAAFVDIGLEKNAFLYIGDLYNFKHNSEDEDVFNAVTKTSIKDVLHVGQ
                     EVLVQVVKESMGSKGARVSMNITLPGRYLVLMPTVDYIGISRRIENEEERHRLKTLAE
                     KIRPPHMGVIVRTVAEGKDIDELKADMEYLVRLWDNIQKKQKGGKVPRLIYKDMNLLA
                     RIVRDIFTPEVNKFYVNTSFGYEKVRELASFISPSLMDRVSLYMGQEDIFEYFNIESE
                     TQRALERKVWLKSGGYIVIDHTEALTAIDVNTGKFVGSIDLEDTVVKTNLEAAKEIAK
                     QLRLRDIGGIVIIDFIDMNSQEHKNMVIETLEAELKKDRTKAHVLGITNLGLVEMTRK
                     KVRQSLDEVLEKVCPYCEGRGRILSEDTMAKKVEREIARIFRTRRGEAILLEVHPSVA
                     AVVIGAGGSRLSYLEQRYGKYIFIKGKDDLHPEEIRIKAVGSKAKLENFAMPVREGQT
                     IDVVLEEAHISNPKNGIARVDGYVIDVEDGADYLGLKVKVQINKTYRTYAKASLI"
     gene            complement(6534..6896)
                     /locus_tag="PK768_01755"
     CDS             complement(6534..6896)
                     /locus_tag="PK768_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59726.1"
                     /translation="MAESENSLKTDLLDMVLEVIQGQRGQSINELISLLALSNLLGII
                     SFLNTQELKYEVKSSQSKSSTSELKEMAATLLASMGTGDKKINPAALINLMKTLSAAD
                     SSEGSAKGSDKSDDKKKD"
     gene            complement(6989..7315)
                     /locus_tag="PK768_01760"
     CDS             complement(6989..7315)
                     /locus_tag="PK768_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59727.1"
                     /translation="MKTFSISENDIQLLKAIRPFMSGKSQKLLDLLLVTINVFKPEEP
                     NQKINYDALNTLLTMVQESIEAKKKHENIIELEEYEEYEEESDNQAEDVENLLNLLAK
                     KQDDES"
     gene            complement(7287..7496)
                     /locus_tag="PK768_01765"
     CDS             complement(7287..7496)
                     /locus_tag="PK768_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59728.1"
                     /translation="MKLKKTNQATVTPDSASSPSPLPNPYTLFLILILLINSFGGINN
                     IFGNIKNILKGKRRYKDEDIFHFGK"
     gene            7622..8095
                     /locus_tag="PK768_01770"
     CDS             7622..8095
                     /locus_tag="PK768_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HQA59729.1"
                     /translation="MGNKSIIILTVILLIAAATGASFLRYNAQAKAKQYALSSWEGDH
                     MLLARIVAGEAEGEPYAGQVGVAAVIINRMRNPRFPSTVADVVYQPGAFESVSNGLIW
                     SRYPSRTHFEAARQALNGWDPTYGCIFFWNPYKPVSRWIWSRPIVTQIGNHVFAK"
     gene            8114..9460
                     /gene="ypeB"
                     /locus_tag="PK768_01775"
     CDS             8114..9460
                     /gene="ypeB"
                     /locus_tag="PK768_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HQA59730.1"
                     /translation="MKKSYIWLISFLVLGGLIFGYWSISNRAFSAENALEASYQRGFY
                     NLLEHVNSLNLLISKSQVTSSDEQRIMLLTTIWHQSEGARNSLASMPLGNRDMTNMQK
                     FFAQLGDFSYRLAEKLVKKQDVTDSDWAKMNQFRKNIQSLSQELRRLQDNVSAGKIRW
                     QDKPDSLFSTRKIQGMGDSLESIDQKLKKEAPTITYDGPFSDHVESPTVKGVTGKLIS
                     ESEAVKIARQVVGKISDWDLKADVAGKTKGTIPAYTIEFRREENQAPDIIMDLSETGG
                     HVIWFFNTRQIGSPKIAIKQAVDNAKAFLESMDYKDFEATGSLRENNTITVTFVPKDG
                     EVLLYPDFIKVEVALDTGEIVGFNALAYLTFHTERNLPQPKLTEEEVRAKLNTNLSVD
                     RIRLTLIPDEALNEKLCYEADVRLNSDRYFIYVNAENGIEERILKVVETDKGTMTM"
     gene            9534..10019
                     /locus_tag="PK768_01780"
     CDS             9534..10019
                     /locus_tag="PK768_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59731.1"
                     /translation="MAKIGDYFQSMDNDGSTMSIAYLLLMANVLGIAVYLDCKKKAKA
                     YTREAEKDMDEETLPPGSTLIKKGKSTTQIDKQLSFDNEKVTELASDNGQNLEKKADK
                     AKVQEKITETNQVEATAEIDNKKQIHTKDNIIDFKEAVASKGKGKKQVLEPLVWRFPG
                     N"
BASE COUNT         3203 a         1820 c         2399 g         2820 t
ORIGIN      
        1 taatattata ctgaactcgt gggttctttt gaacccattt tttttgtaat aaagaggcct
       61 tgcggataat agatatataa aaaagggggg ccctttatgt attactatga agaagcttcc
      121 gaaaagaact ggtttagacc tgagaatcta aaaagaatca ccatctgtat tattttcatt
      181 attgtagttt tattgctgaa aaaggtgaac atgcccatat tcaagactgc cctttcaaaa
      241 attgagtatt acctttgcga ttactcctac gagtatcaag atatagtgga agctgtaaaa
      301 aatatttcag aagctccgga taaaataccc gtcttaagcc agggaagcag taagcttttg
      361 cccatgccgg tccatggcag tatctcttcg gagtatggct tgcgacttca tcccatatta
      421 aaacagcaac agatgcacaa gggcatagac atagttgaga aagaaggagc tcctgttaaa
      481 gttgttatag atggcgtggt ggattctgtg ggctatgatg aggaactcgg aaatacggtt
      541 aagataagcc acggcaacgg tttgacaacg gtttatgccc atttaaagga tgtttatgtg
      601 aaagagaaag aaactgtaaa gcagggcttt ataatcggga caatcggcaa caccggcctt
      661 tcagaaacag cacaccttca ctttgagata tggaaggatg gcaaagcaga agacccaaga
      721 aaatggctca atatacagga taacactcgg gaggacttag atggttttaa gggtctctaa
      781 ttgtaaagta agcataagtt cgttatttat tgcggctatt gtcctggccc ttgcagcggg
      841 tatgcacata gagatgctgg cagcattgtt tgcactggga gttcatgagt ttgcccatgt
      901 atttgcgggc agcagactcg gcatccctat tcatgaaata gagatactcc cttttggggg
      961 taggatcata tccggtataa gtgatgcaac tgatgaaagt gaggttataa tggttttggc
     1021 cgggccgctg gcgaactttg cagcagcagg gtttatgcta ggcctttcat atttcgcaat
     1081 tttacctgca aaaatcgcct ggcaattcat caactaccag ctcatgctgg gggtttttaa
     1141 catgctgccg gccttccctc ttgatggggg gaggattttt gcattatggc taagcaagca
     1201 catgactttt acatcatcgg tgcgcattgc atcacatgct ggcaagatac ttgcatattc
     1261 attgctgctt actgcattta ttggaattat ctttaaaaag attaacttta gtttaataat
     1321 aacaggtttt tttttattgc tgcaggcttc aaaggaggag gagaacgccc atcttatttt
     1381 catgaattat ttggcaaaaa aaaaggagaa actattaaaa aaccaatact ttcccggtga
     1441 aattgttgta gttgaccagg aagctacggc aaaaaagata ttatatttat ttttaccaaa
     1501 aaaatatttt atagtttata ttttggatag aaacatggaa atacgaaaaa ctttaacgga
     1561 gacggaaata tttgacatta taatggaaaa aggattggac tttaagatga aagagttgat
     1621 ataataatta atacaaataa tatctcaatt gagaccaaag ctaggaggca ctgttttgac
     1681 catagaaaat caactggaaa aactattgtc acgggtatca aaacctaccc gctatatagg
     1741 gaacgaatac aacagtataa gaaaagacct tgacaacata agagttcaca tagcacttgc
     1801 atttccggat gtatacgagg tgggtatgtc ccatctgggt ataaagattc tgtatcatct
     1861 cctaaatgaa tatccggata tttatgcaga gagagtttat gctccttggg ttgattttga
     1921 agatttgatg aggaaagagg gtgttcctct ttttagtttg gagagcaaaa cctccatttc
     1981 ccaattcgat tttttggggt ttaccctgca atatgaattg agctatacca acatagtgaa
     2041 tatgttggag ctctcaaacg tacctatttt ttcaagggag agaaatgaca gccatccttt
     2101 tgtgatagcg gggggcccct gtgcctttaa cccggaaccc ttggctgaca ttatagactt
     2161 ttttgttata ggagaggctg aagaagccat attagaggtc atagacttat ataaagactt
     2221 tatggagagg ggtgaaagtc gacagaagtt cttgtgcaat gtggctcgga ttcccggagt
     2281 ttatgtgcca tctttgtata gtgcatcata taatagtgat gggacaataa gcagtatagt
     2341 accgataaaa gaccatattc cgaagcgtgt tcaaaagaga gtaatacaga atctggacaa
     2401 tgtttattat ccgactaagt ttatcgttcc ctatatggac atagtccacg accgggcttc
     2461 cttggagata tttcgaggct gtacaagagg ctgccgtttt tgccaggcag gtatgattta
     2521 tcggccggtc agggaaaggt cggtagggcg cctggaaaaa ctagcaaagg atatcatatc
     2581 atcaacgggc tatggagaaa tctctttatt gtcccttagc agcagcgatt attctgacct
     2641 aaaacgactt gccgaactgc tgacggaaag ttttagggag agtaaggtgg gtttatcgct
     2701 gccctccctt agaattgacg ctttttcgtt gggccttgca gaaaaagtcc aggaaatcag
     2761 aaaatcgggg ctaacttttg cgcctgaagc cgggacccag aggctgagag atgtaatcaa
     2821 caaaggcgta actgaggaag atcttttgtc ttcagtaaga gatgcctttg ctttgggctg
     2881 gaacaaagtt aagctgtact tcatgatagg ccttcctact gaaactgacg aagacataaa
     2941 gggcatttcc gagcttgccc atgctgttgt cgatgtatat cggcaggtaa acggtcatac
     3001 caagggcctg acggttaccg tcagtacgtc taccttcgtc ccaaagccgt ttacgccctt
     3061 tcagtgggaa agtcaaatta cgctggaaga aatctatgaa cgtcaaaatt tgctgaaaaa
     3121 gctgctcaga aaaaatagga atatttctta cagatggcac gatggcaaac taagcttttt
     3181 agaagcggta ttttcaaggg gtgaccgaag actcggcaat gttttaaaaa ttgcccatga
     3241 aaagggctgt aagtttgata gctgggatga attctttaat tttgataagt ggatgtctgc
     3301 ttttgaagag gctgaaattg atcctgactt ttatgcatat cgcaaaaggt ctgaatctga
     3361 aaagttcccc tgggaaatca tagatgccgg tatcgataga aagtttttgc ttagagaatt
     3421 ggaaagggca aaaagaggac aaataacccc cgactgcagg ataagctgtt acggctgcgg
     3481 gattaaaaga ctgggagatg gtttctgtga agctaagatt gaggtttaaa aaagagggac
     3541 tggccaggtt catttctcat ctagacctga tgaggacttt tgaacgggct ttcagaaggg
     3601 ctgagttgcc catagctttc agccagggct ttaatccgag gccaaagatg acttttgctt
     3661 cggcattgtc agtaggaatt agcagtagca gcgaatattt agatgtggaa tttagtgaag
     3721 atgtctctgc cagcgatgta gcattaaagc taaatcctgc cctgcctgag gggatcaaag
     3781 taaccgatgt gaaaatggca gatgaagcta ttagcctttc catgttaaac ggtgccaagt
     3841 atgttgtcag gctagcttta aatgaggtgg atttagagga actaaaaaaa agcacaaaag
     3901 gccttttaga acaaaaggag attataatag aaaaaaggac caaatccggt aaaaagttag
     3961 taaatattgc tccttttatt tatagcctgg gtttatcaaa ttgtgatggg agtgctgcgg
     4021 atattgtcat gtcagttgca atcggacagc aggggagcgt tgcacctacg atggtaatta
     4081 atgaattgga aaggatactg tgcaaagaac ttgagattgt cagcatacac agggaggcaa
     4141 tcttttacaa gagcaatgga aaggaaattt tacccatgta ataaagcagg aagcatattt
     4201 aatttttggg ggatttgagg agctgaaaat ttgatgtcaa aggagatttt agcagatgtt
     4261 gatcgggatc agatacgcat tggggtgcta gaagataggc agttagttga atactatgtt
     4321 gaaaggaccc atgatgaaag ggttataggc aacatctaca aggggaaagt tgccaatgta
     4381 cttccgggaa ttcaagctgc ttttgtggat ataggattgg agaagaatgc atttttatat
     4441 attggggatt tatataattt taagcataat tctgaagatg aagatgtctt taatgctgtg
     4501 acaaagacat ctataaaaga tgtattacac gtaggccagg aagttttggt acaggttgta
     4561 aaagagtcaa tgggcagcaa aggggcccgg gtgtcgatga atattacgct gccgggacgg
     4621 tacttagtat tgatgccaac agtagattat ataggcattt ccagaaggat agaaaatgaa
     4681 gaagagcgcc atcgcttaaa gacccttgca gaaaaaatcc ggccacccca catgggggtt
     4741 atcgttagaa cggtcgcaga gggaaaggat atcgacgaat taaaagctga catggagtat
     4801 ttggtacgcc tttgggataa tatacaaaaa aagcaaaaag ggggaaaagt cccgcgcttg
     4861 atatataaag acatgaatct tctcgcccga atcgtaagag atatttttac tcccgaggtg
     4921 aataagttct acgtcaatac gtcgttcgga tatgagaagg tccgggaatt ggcctcattt
     4981 atatcgccat ccttaatgga cagggtgagc ctttatatgg gccaggagga tatctttgaa
     5041 tactttaata ttgaatcgga gacccaaaga gctcttgaaa gaaaggtgtg gcttaagtcc
     5101 ggcggatata tcgttattga ccacacggaa gctttaactg ccattgatgt aaatacaggc
     5161 aagtttgtgg gaagcatcga tttggaggat acggtagtca agacaaatct ggaagctgca
     5221 aaagagattg caaaacagct gagacttcgc gatatcggtg gaatagtaat aattgacttc
     5281 atagacatga attcccagga acacaaaaat atggtcatag agaccctgga agctgaactc
     5341 aagaaggata ggaccaaggc ccatgtcctt gggattacta atttgggcct tgtggagatg
     5401 accaggaaaa aggtccgtca gagcctcgat gaagtattag aaaaagtgtg cccttattgc
     5461 gagggaagag gcagaatatt atcagaggat accatggcta aaaaagtaga gagagaaatt
     5521 gccagaatat ttaggacccg ccgaggtgaa gctattttat tggaggtcca tccctcggtg
     5581 gcagcagtag taattggggc cggtgggagc agactcagct atcttgagca gcgatacggg
     5641 aagtacattt ttattaaagg caaggatgat cttcatccgg aagaaattcg cataaaagcc
     5701 gtgggaagca aagccaagct cgagaatttt gcgatgccgg ttagggaagg tcagacaata
     5761 gatgtagtac tcgaagaagc gcatatatcc aatcctaaaa acggtatagc aagggttgac
     5821 ggctatgtta tcgatgtgga agatggagcc gactacttag gtcttaaagt gaaggttcag
     5881 ataaataaga cttacaggac ttatgcgaaa gctagtctca tttaatactt agggctcccg
     5941 gagtaattcc gggagcccta aattttttac aattctattt ttacaaatct gcctttttag
     6001 aacctgataa tactgttttt catatattat agcagaggca atgataggga tattgtcagc
     6061 agataatagg cagtaacccc aaaataagtc attgcatata ctatatcagg tcacaaaaaa
     6121 ggagggaatt ttaaatggca gacatccaag gcggtggctt ctttggtagt cgttgggcct
     6181 ttgccctgtt tcttatatta atacttctgt ttttcgctga tgattaagtc gatatgccgt
     6241 gctttttgca cggctatttt gaaaatactt aaggagggag tcttagatgg ctgatcttca
     6301 aagtggtggc ttcttcggca gccgttgggc ctttgccctg tttctaatac tgattctcct
     6361 gttctttgcc gatgaatgag gtaatgttaa ggagaggagg gaacaaggat gggtggagga
     6421 cctgtcggaa aaggctcaag tggaaacaga tgggcatttg ctctgttttt aatactagtc
     6481 cttttgttct ttgcagatga ctaagaaaat ggccgtgtcg ttcgtgacac ggcctagtcc
     6541 tttttcttat catccgattt atctgagccc tttgcactgc cttcgctgct gtcggcagct
     6601 gatagagttt tcatcaaatt tatcagggct gcaggattta tttttttgtc tcctgtcccc
     6661 atggaagcga gtagtgtcgc agccatctct ttaagttcgg atgtgctgct ttttgattga
     6721 gaactcttta cttcatattt aagctcttgt gtattcaaaa agctgataat tccaagcaga
     6781 ttgctgagag ccaacaggct tatcaattca ttgatgcttt ggccgcgctg gccctgtatc
     6841 acttctaaaa ccatgtcaag caaatcggtt ttgagcgagt tttcactttc tgccaaaagc
     6901 gcacctcctc atgtgtgaaa gttaagatat cttatatgaa tatatgctgc agtatataaa
     6961 aaaagaaccc aaggttgttc acaacatatt aactttcatc atcttgtttt ttggccagta
     7021 gattcaacaa attctcaaca tcttctgctt gattgtctga ctcttcttcg tattcttcgt
     7081 actcctccag ctcaataatg ttttcatgtt tttttttggc ctcaatggat tcctggacca
     7141 ttgtcaatag cgtgtttaaa gcatcgtaat taatcttttg attcggctct tcaggtttga
     7201 acacatttat tgtaacaagt aaaaggtcca gcaatttctg gctctttccg ctcataaaag
     7261 gccgtatagc ttttaaaagc tgtatgtcat tttccgaaat ggaaaatgtc ttcatcttta
     7321 tacctcctct ttccctttaa aatgtttttt atattgccga aaatattatt tatacctccg
     7381 aaggaattta ttagcaggat aagtatcaga aaaagagtgt acggatttgg cagggggcta
     7441 ggactactgg cggagtcagg ggtaacggtt gcttgatttg ttttcttaag cttcacttac
     7501 ttcccctcct aagacttttc tctaaattat tatatgaagg gagcaatcaa aaaggtactg
     7561 gaataagctt ttcaaaaata agcaaactat ggacaggaat atataaagag ggtgattatc
     7621 tatgggaaat aaaagtatca tcatcttaac agttatactt ttaattgccg cagcaacagg
     7681 agcttctttt ttgcgctata atgcccaggc caaggcaaag caatatgccc tttcctcttg
     7741 ggaaggggat cacatgctgc tggcgcgcat tgtagcaggt gaagctgaag gggaacctta
     7801 tgcggggcag gtgggcgtag cggctgtaat tataaaccgc atgagaaatc caagatttcc
     7861 ctcaacggtt gccgatgttg tatatcagcc aggggctttc gaatctgtaa gcaatggttt
     7921 gatatggtcc aggtacccat cccgaactca ctttgaagct gccagacagg ccctgaacgg
     7981 atgggaccct acttatggct gcattttttt ctggaatccc tataaaccgg taagcagatg
     8041 gatatggtct cggcccatag tgacccaaat aggaaaccat gtatttgcaa aataaattgg
     8101 gagggcaata acattgaaaa aatcctatat ctggctaatt agttttttgg tgcttggcgg
     8161 tctgatattc ggatactggt ctatatcaaa cagggccttt tccgcggaaa atgccctgga
     8221 agcatcttat cagcgcggct tttataattt actggaacat gttaacagct taaacctttt
     8281 gatttcgaaa agtcaggtca cctcctcgga tgaacaaaga ataatgctgc tcaccactat
     8341 ttggcaccag tcggaagggg cgaggaatag ccttgcgtcg atgcccttgg gaaatcggga
     8401 catgacgaac atgcaaaagt tttttgccca gctaggagac ttttcatacc gccttgccga
     8461 aaagttggtc aagaaacaag atgttacgga cagtgactgg gcaaagatga atcagtttag
     8521 aaagaacatt caaagcttaa gtcaagagct aagacgcttg caggacaatg tatcagcagg
     8581 aaaaataaga tggcaggaca agcctgacag cctttttagc acgcggaaaa ttcaaggcat
     8641 gggggacagc ttagaaagta tcgaccaaaa attaaaaaag gaagcgccca ccattaccta
     8701 tgatggccca ttttcggacc atgttgaaag ccctactgta aaaggagtga ccggtaagct
     8761 cataagcgaa agtgaagctg tcaaaatcgc ccgccaagtt gtaggaaaga taagcgattg
     8821 ggatttaaag gctgatgtag ctggaaagac aaaagggact attcctgcat atacaattga
     8881 attcagacgt gaggaaaatc aagctccaga tatcatcatg gatctaagcg aaacgggggg
     8941 ccatgtgatt tggtttttta atacccgaca gataggcagt cccaaaatcg ccattaagca
     9001 ggcagtagat aacgctaagg ccttcctaga atcgatggat tacaaagact ttgaagcaac
     9061 cgggtcttta cgggaaaaca ataccataac ggtgacattt gttccaaaag atggtgaggt
     9121 gcttttgtat cccgacttta ttaaagtgga agtcgctcta gatactggcg agattgtagg
     9181 ttttaatgcc ctggcatatt tgacatttca tacggagcgc aatttacccc aaccaaagct
     9241 aactgaagaa gaagtcaggg caaaattaaa tacaaacctc agcgttgaca gaatacggct
     9301 tactctaatt cccgatgaag cactaaatga aaagctgtgc tatgaagcgg atgtgaggct
     9361 aaattctgat agatatttca tttacgtaaa cgcagaaaac ggcattgaag agagaatcct
     9421 aaaagtggta gaaaccgata agggcacaat gacaatgtaa aaaattgtga cgaataaaac
     9481 cccaccggca tagtatatat ataaggtgaa aaatttgctg gaggggtttt tttatggcca
     9541 aaataggcga ttatttccag agcatggaca atgacgggtc aaccatgtca atagcatacc
     9601 tgctactcat ggcaaatgtg ttgggcatag ctgtgtacct tgactgcaaa aagaaagcaa
     9661 aggcatacac ccgagaagcg gaaaaagaca tggatgaaga aactttaccc ccaggttcta
     9721 cattaattaa aaaggggaaa tcaactacac aaatcgataa acaattatct tttgacaatg
     9781 aaaaggtcac agagcttgca agtgacaacg gacaaaactt ggaaaaaaag gctgataagg
     9841 caaaagtcca ggaaaaaata acagaaacaa atcaagtcga agcgactgct gaaatagata
     9901 acaagaagca aattcatacc aaggataaca ttatcgattt taaagaagca gtggcgtcga
     9961 aagggaaggg aaagaaacag gttcttgaac ccttggtttg gagatttccc ggtaattaat
    10021 ttgttgattt acatagtgta aaattacttt gggtaaaaac caaaaaataa aagataagag
    10081 tatctcctct gtgatatgat gtaatctgca agaaccacaa aaatcacaga aaaggagata
    10141 cccttatgga actaattata catgaaatca tacaaaaaat cagtagcagt tttgaaaaag
    10201 agctagaaaa actaataaga gaaaaaagag acatatccga at
//
LOCUS       DAONUO010000028        10104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5104_length_10104_cov_3.530799, whole genome shotgun sequence.
ACCESSION   DAONUO010000028 DAONUO010000000
VERSION     DAONUO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5104_length_10104_cov_3.530799"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PK768_01785"
     CDS             complement(<1..650)
                     /locus_tag="PK768_01785"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQA59732.1"
                     /translation="MSSIVIEGGRPLKGTVKISSAKNAVLPVIAASLLTESECIIEDA
                     PELEDVKVMTEVLNSLGAKALREGDSIKISAGTIDSFEAPYDLVRKMRASFLVMGPLL
                     ARKGRARISMPGGCAIGSRPIDLHLKGFAALGANIELGHGFVEAKCDKLKAATIYLDF
                     PSVGATENIMMAAALAEGQTTIGNAAKEPEIIDVANFLNSMGAKIRGAGTDVIKIEG"
     gene            complement(689..1435)
                     /locus_tag="PK768_01790"
     CDS             complement(689..1435)
                     /locus_tag="PK768_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HQA59733.1"
                     /translation="MKLVKSLIVFILITLLTPGVTLSRDNEQVLQEALNAAGAEPDMI
                     DIVDWSVINREFLEFAQMENCRDKIIDIFEIGEKTFDIIKENSESYRILNTRVQLDSE
                     TFLHIILQSVRLPEEYEKEPQTYLVVNVSGRKFDNFVYYGKKVQEAVLSLGGESKITS
                     CVTGYFNGKLDEAEQDQILENIYDCLKISHKQIMKDEYTFSLMGYSPLFVDCIEIMDK
                     CYNVNIAIRYNLEDDKTYIWIGIPVISIEY"
     gene            complement(1669..2295)
                     /locus_tag="PK768_01795"
     CDS             complement(1669..2295)
                     /locus_tag="PK768_01795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQA59734.1"
                     /translation="MQTRVNPTRMELTRLKRQIVIARRGHKLLKDKQDELIKKFVDMV
                     KENKRLREEVEKELQLAFKNFIIARSQMPQEVLEESLMIPSARVSIDVSKENIMSVNV
                     PKIKINKEEGGNIYSYGLATTSAELDSAITILSRMLEKMLKLSEIEKACQLMADEIEK
                     TRRRVNALEYVMIPTMEENIKYITMKLDENERSTITRLMKIKEMVMEA"
     gene            complement(2298..3680)
                     /locus_tag="PK768_01800"
     CDS             complement(2298..3680)
                     /locus_tag="PK768_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQA59735.1"
                     /translation="MIKEYRTAREIAGPLMMVENVEGVKYNELVEIETGSGEIRRGQV
                     LEVDGDKALVQLFEGSTGLNINDCKIRFLGKSIQLGVSIDMLGRVFDGSGRPKDNGPK
                     IIPEKRLDIDGNPINPTARDYPSEFIQTGISAIDGLNTLVRGQKLPIFSGSGLPHAQL
                     ATQIARQAKVLGTESKFAVVFAAMGITFEEANYFISDFRRTGAIDRSVLFINLADDPA
                     IERISTPRMALTCAEFLAFEKEMHVLVILTDMTNYCEALREVSAARKEVPCRRGYPGY
                     LYTDLSTIYERAGRLKGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSCELY
                     RKGVMPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYARGKQAKELAVILGEA
                     ALSDTDKLFAKFADEFETRYVSQGENEDRSIEETLNLGWELLTILPKTELKRIRDEYI
                     EKYLPKKGEE"
     gene            complement(3673..5445)
                     /locus_tag="PK768_01805"
     CDS             complement(3673..5445)
                     /locus_tag="PK768_01805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016469 - proton-transporting two-sector
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit A"
                     /protein_id="HQA59736.1"
                     /translation="MTNGVVVKVSGPLVVATGLPQAKMFDVVRVGQQGLIGEVIEVHG
                     EKLSIQVYEETSGLGPGDPVVSTGVPLSVELGPGLLERMFDGIQRPLEAIEKQAGSYI
                     TRGIDIPALDREKKWDFVPTAKQGQKVTGGDIIGTVQETVIVEHRIMVPPGISGEIVE
                     IYEGSFTVTDTVAKIKTDSGEIIDVAMMQKWPVRMTRPYKEKVPPEEPMSTGQRVIDT
                     FFPVTKGGTACIPGPFGSGKTVVQHQLAKWADAEIVVYIGCGERGNEMTDVLLEFPEL
                     IDPKTGEPLMKRTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLSRRLAEFYERAGRVMTIGSEEREGALTVVGAVSP
                     PGGDLSEPVTQATLRVVKVFWALDASLAYARHFPAINWLTSYSLYSDTVEEYMSKQIA
                     PDWKDLRTEAMRLLQEESSLQEIVRLVGIDALSIKDRLILEVARSIREDFLHQNAFHD
                     VDTYTSLNKQYRMLKLIFMFYEEAQKAIEKGVVFSEIENLPVRERIARAKYSDEKDTH
                     IFDEIEKELKQQIETLMEGGSLDD"
     gene            complement(5442..5777)
                     /locus_tag="PK768_01810"
     CDS             complement(5442..5777)
                     /locus_tag="PK768_01810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HQA59737.1"
                     /translation="MSTYKMAVVGDRDTVLAFKSLGVDVFPVLNEREANFTITRLANE
                     NYAVIFVTEQIAKEIPEKIAQYRSQMLPAITLIPSNRGTLGIGIGEVKKSVEKAIGAD
                     ILFGREGEE"
     gene            complement(5764..6765)
                     /locus_tag="PK768_01815"
     CDS             complement(5764..6765)
                     /locus_tag="PK768_01815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HQA59738.1"
                     /translation="MAKDTDFLYVSARIKHLETKLLGRSDIERILDAPGPEEAVKVLS
                     DTEYGSDLAEMENIYEFEKVLDKSMIRTIRTLKESFSNHEFIRFFTMKHDFHNLKVIV
                     KAMISGTENGNLSHLAEIPPEEIKKYVQEETGADIPDELKAAYDRAVEAYELTKDPQQ
                     IDFALDQALFDNLEEIVKVSRDSFLQEYLTALSDLTNIKIMMRLKKMDADIRTLDNAL
                     VSGGSLSKEFFKEKYFESSQSLIDALDNTPYKQLVEEGISQWESTGSPSLFEKLIDNF
                     LISLARRGLYKPFGSETVIGYLAARENEIKLLRIIMVGKINGISSDMIRERLRDVYV"
     gene            complement(6770..7372)
                     /locus_tag="PK768_01820"
     CDS             complement(6770..7372)
                     /locus_tag="PK768_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HQA59739.1"
                     /translation="MEGIAKIKERILEEAREEKEKIISDANAQAQNIVKQYEQKAKDI
                     LDDILEKAGKTAEEKRRRILSMAQLENRKQLLLAKQQIIDEVFERAKTSLKNVPLEKY
                     QTLIENMLLSSVITGNEEIIIAEHDKNRITPDFIQKINNTLSGMGKQGNLRISKTYGN
                     MIGGFVLKSQDLEINSTFDSLIDIEREELETEIAKILFEE"
     gene            complement(7386..7865)
                     /locus_tag="PK768_01825"
     CDS             complement(7386..7865)
                     /locus_tag="PK768_01825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HQA59740.1"
                     /translation="MSLTLGQVLALFGAALAVGLPGIGSARGVGIVGESAAGLVTEDP
                     GKFGQALILQALPGTQGIYGFLAGFLTIQKIGLLAGTLVDITPYQGWLIFASCMPIAI
                     VGFLSAIYQGRVAAAGIGIVSKRPEEVAKGITFAAMVETYAVLALLATILMLFGINV"
     gene            complement(7904..9880)
                     /locus_tag="PK768_01830"
     CDS             complement(7904..9880)
                     /locus_tag="PK768_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HQA59741.1"
                     /translation="MPQDESVQQAWQWLVPPVVGDEHGRLIPRKQVTAKTPSRLNPEV
                     QPTLDFVEDIEQAGEVQKNYVNQELNRELQELETKLAELNFGIDFLKPYVKSTNPLIH
                     GKPSIKKDELSEILSKESSILDNLQAVFDLDKQLSQLKTEETRLIGQIEMLSPWRALD
                     IPLEELGETRKTVFEAAVMPRKSRAELDRIITEEGLLAEIATLGESQDDVYTLVVYHK
                     EHKENMEKLFKEFSVNLQDFSNFEGTPKELIKAAEKRIEDIEVERGTIKEKSKELSDN
                     LRYFQILYDYYTVEKDRKSAFLKVTDTDKAFSMQAWLPEEHEELVKSTVSKVTDTVYM
                     EFSAPTDEDDMPVLLSNAKLVEPFEVITELYSLPDPRGLDPNLYMAPFFFVFFGMMLS
                     DAGYGIIMSLLAGYALFRFKLAGSGKKFTAMLFLCGISTVIWGAIFGGWFGDLIRVKP
                     MWINPLDNPLSVLILSFVMGVVQIYVGIILNAYKNIRAGKVADALMDQGLWLVLLTGL
                     LMFVMPELAQLGKYMAIFGAVGLILTQGRSQKGIIKKFTSGVLSLYDITGYLSDVLSY
                     SRLLALGLTTGVIATVINTMAKTVAGSIIGYIVMVLIIVGGHVFNLAINVLGAYVHSS
                     RLQYIEFFSKFYDSGGRAFDPLRVKTSYVDLEDI"
     gene            complement(9894..>10104)
                     /locus_tag="PK768_01835"
     CDS             complement(9894..>10104)
                     /locus_tag="PK768_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59742.1"
                     /translation="MTGASVVPVAVTSAKCWPRKAFIKKPGVVDVSIGKPISSVGRKH
                     DELMAEVQAWIESEMQRLDPEAYRK"
BASE COUNT         2456 a         2492 c         1993 g         3163 t
ORIGIN      
        1 ccttcaattt taatgacatc ggtaccggca ccccggatct tggctcccat ggagtttaag
       61 aagttggcaa cgtcgattat ttcaggctcc ttggctgcgt ttccaatggt ggtctgtccc
      121 tctgccaggg ctgcagccat cattatattt tccgttgctc ctacactggg aaaatccagg
      181 tatatagtcg cagctttgag cttgtcgcat tttgcctcta caaatccatg acccaactca
      241 atattggctc ctaatgcggc aaaacctttt aaatgtagat ctatgggcct tgaaccgata
      301 gcacaacctc cgggcatgga tattcgggct cgtccctttc ttgcaagcaa gggacccatt
      361 accaaaaaag aagccctcat ttttcgcact aaatcatagg gggcctcgaa actatcaatt
      421 gtaccggcac ttatctttat agaatctcct tcccgcagag ctttcgctcc caaggaattt
      481 agaacttccg tcatgacttt gacatcttca agttcaggcg catcttcaat aatgcattcg
      541 gactcggtga gcagtgaggc ggctataacg ggcagtactg cgtttttggc actgctaatt
      601 ttgaccgtgc cttttagggg cctgcctccc tcaatgacaa tgcttgacaa cgacttcccc
      661 tccgattgta cagtatacta gtagtgtttt aatattctat tgaaataacc ggtatcccaa
      721 tccatatata cgtcttatca tcttccaagt tatatctgat ggcgatgttt acattgtaac
      781 atttatccat aatttctatg caatctacaa ataaggggct gtatcccata aggctaaaag
      841 tatactcgtc tttcattatt tgcttgtggg aaattttaag acaatcgtaa atattctcaa
      901 ggatttggtc ttgctcggcc tcatctagtt taccattaaa atagcctgta acacaagagg
      961 taatttttga ctctcctcca agtgacagca cagcttcttg gactttcttc ccatagtata
     1021 caaaattatc aaatttcctg ccacttacat ttactaccag gtacgtttga ggctcttttt
     1081 catattcttc aggtagtcga acggattgaa gaatgatatg taaaaatgtt tcggaatcta
     1141 actgaactct agtgtttaat attcgatatg attcgctgtt ttcctttata atatcaaaag
     1201 ttttttcccc tatttcgaat atatctatga ttttatcgcg acaattttcc atttgcgcga
     1261 attcaaggaa ctccctgttt attacagacc aatcaacgat atctatcata tccggctctg
     1321 cacctgctgc atttaatgct tcctgcagta cttgttcatt atccctactc aaagtcactc
     1381 ccggagtcaa caaggtaatt aaaataaata caataaggct cttcacaagc ttcattaaag
     1441 ccacccccca tacttacaag tattctctag attctattcc cctcattcaa gattattacc
     1501 cctttgccca aacttatgct tagattctag tatgtgccat atatttcctt cagtgaatac
     1561 tgcctgtttt tgtctgttgg agcacaattt ttttgcattt gccgactttt tagtttgttc
     1621 atttaaaaaa ccccctactg cttttttcgc agtagagggt ttttaattct aagcttccat
     1681 aaccatttct ttaatcttca tgagccttgt tatggtgctg cgttcgtttt catccagttt
     1741 catcgttata tattttatat tttcttccat ggtcggtatc attacatatt ctagcgcatt
     1801 taccctgcgg cgggtcttct ctatttcatc ggccatcagc tgacaggctt tctcgatttc
     1861 tgatagtttc agcatctttt ctagcatcct tgacaatatg gttatggcgc tgtccagctc
     1921 tgccgatgta gtagcaaggc catatgaata tatgttgccg ccttcttctt tgttgatttt
     1981 aatcttgggc acattgacgc tcataatgtt ttccttgcta acatcaatac tgacccgggc
     2041 agagggaatc atcaagctct cttctaatac ttcctggggc atttgactcc gagctattat
     2101 gaagttcttg aaggccaatt gcaattcctt ctcaacttcc tcccgaagcc ttttgttttc
     2161 tttgaccata tcgacaaact tttttattaa ttcatcctgc ttgtctttta atagtttgtg
     2221 cccgcgccgg gctatgacta tctggcgctt cagccgcgta agctccatcc gggttggatt
     2281 tacccgagtc tgcatgttta ctcttctcct tttttaggca ggtatttttc gatatactcg
     2341 tctcgaatac gcttgagttc cgtcttcggc aaaattgtca agagttccca ccctaagttg
     2401 agggtctctt ctatgctcct gtcctcgttt tctccctggg aaacgtaccg cgtttcaaat
     2461 tcatcggcaa attttgcaaa gagcttatcc gtatcgctga gggccgcttc accgaggatt
     2521 actgccagct ccttcgcctg cttacctctg gcatatgccg caaacagctg gttcatggtg
     2581 tcggcatggt cttccctggt cttgcctttt ccgataccct tgtctttcag ccttgatagt
     2641 gaaggtaaca cgtctattgg cggcattaca cctttcctgt aaagttcgca gctcaaaatt
     2701 atctggcctt cggtaatata acccgtcagg tcaggaattg ggtgggtctt atcatcttcg
     2761 ggcatggtca gtatcggtat ctgtgtgatg gatccttttt tgcccttgag acgacctgcc
     2821 ctttcatata tagtggaaag gtcagtatat aggtatcccg gatagccacg gcggcaaggt
     2881 acttcctttc gggcagctga tacttcccga agtgcttcac agtagttggt catgtcggtt
     2941 aagataacca ggacgtgcat ttccttttca aaggctaaaa actctgcaca ggtaagggcc
     3001 attcggggag ttgaaatacg ttcaattgca gggtcatcag ctagatttat gaaaagcacc
     3061 gaacggtcaa tggcacctgt cctcctaaag tctgatataa agtaattggc ctcttcaaag
     3121 gttatgccca tggcagcaaa taccacggcg aatttgctct cggtgcccaa gactttagcc
     3181 tgtcttgcaa tctgagttgc tagctgtgca tggggcaagc cggagcctga aaagatggga
     3241 agcttttgac cccgcacaag agtgtttaag ccgtcaattg ctgaaatacc ggtctggata
     3301 aactccgagg gatagtccct ggccgttggg tttatggggt tgccgtctat gtcaaggcgt
     3361 ttttccggaa tgatcttggg gccattgtcc ttgggccttc ctgatccgtc aaagacccta
     3421 cccagcatat ctatagatac gcccaactgg atgctcttgc ccaaaaatct tatcttacaa
     3481 tcatttatgt taagaccagt tgagccttca aaaagctgga ccaatgcctt gtcgccgtca
     3541 acctctaata cctgtcctct gcgtatctct ccgctacctg tttcaatttc aactagctca
     3601 ttgtacttaa caccttcaac attttccacc atcataaggg gaccggcaat ttcccgtgcg
     3661 gttctgtatt ctttaatcat ctaggctccc cccttccatt agcgtttcaa tctgctgctt
     3721 taactctttt tcgatttcat cgaagatatg tgtatctttt tcatcggagt attttgccct
     3781 ggcaattcgt tcccgaacag gtagattttc tatttcggag aacacaacac ctttttcaat
     3841 ggccttttgg gcttcttcat agaacatgaa aatgagcttt aacatcctat attgtttgtt
     3901 caatgaagta tatgtgtcca catcatggaa ggcgttttga tgcaaaaagt cttccctgat
     3961 ggaacgggcc acttccagta taagtctgtc ctttatcgaa agggcatcaa tacccaccag
     4021 tcggacgatt tcctgcaaac tggactcttc ctggagaagc ctcattgctt ctgtcctcaa
     4081 gtctttccag tcaggggcta tttgtttgct catatattct tcgacagtat cggaatacag
     4141 tgagtaactt gtcagccagt tgatggccgg gaaatgcctg gcatatgcca gggaagcatc
     4201 tagtgcccag aataccttta ctactctaag ggttgcctgg gttaccggtt ctgaaaggtc
     4261 accgcctgga ggagaaacgg cacctacgac ggttagggca ccttcccttt cctcactgcc
     4321 gatggtcatg actctgcctg ccctttcata aaactccgca agccttcggg aaaggtatgc
     4381 gggatatcct tcgtcaccgg gcatttcttc aagtcgaccg gacatttctc taagggcttc
     4441 tgcccaacgg gaggtggaat cagccatcaa tgcaacactg tagcccatgt ccctgaagta
     4501 ttcggctata gttatgccgg tgtatataga agcttctcta gctgcaacgg gcatgttgga
     4561 tgtgttggca atgaggaccg tccttttcat caggggctca ccggtcttgg ggtcgataag
     4621 ttcagggaat tccaagagaa catcagtcat ctcgttgccc cgctcaccgc agccgatgta
     4681 aacaacgatt tctgcatcgg cccattttgc tagctgatgc tgtactactg tttttccact
     4741 gccaaaaggc cccggtatac atgccgttcc accttttgtg actgggaaaa aggtgtcgat
     4801 tactctttgc ccagttgaca tgggttcttc cggcggaact ttttccttat aaggccgtgt
     4861 cattctaaca ggccatttct gcatcattgc tacatcgata atttcgccac tatcagtttt
     4921 tatctttgca acagtatctg taacggtaaa gctgccttcg tagatttcta ctatctcacc
     4981 ggatattccg gggggcacca ttatgcggtg ctcaactatt accgtttcct gcaccgttcc
     5041 tatgatatcg ccaccggtaa ccttctgacc ctgcttggcg gtgggtacaa aatcccattt
     5101 tttctctcga tcaagagcgg gtatgtcgat acctcttgta atataacttc ccgcttgctt
     5161 ttcaattgct tcaagggggc gctggattcc atcaaacatc ctctccagca gtcccggtcc
     5221 cagttcaacg ctaaggggaa cacctgttga aaccaccgga tccccggggc ctaagcctga
     5281 tgtctcttca taaacctgta tggaaagttt ttcaccatga acttctataa cttcaccgat
     5341 aaggccttgc tggcccactc tgaccacgtc gaacattttg gcttggggca gtcccgttgc
     5401 cacaacgagg ggaccggaaa cttttactac tacaccgttt gtcattcctc tccctctctt
     5461 ccaaataaga tatctgctcc aatggctttt tccacggatt ttttcacttc tcctattcca
     5521 attccaaggg tgccccggtt acttggaata agggtaatag caggaagcat ctggctcctg
     5581 tactgggcta ttttttccgg gatttccttg gcaatctgct ccgtaacaaa aatcaccgca
     5641 tagttttcgt tcgcgagcct ggtaattgta aaattggctt ctctttcatt caagacggga
     5701 aagacgtcaa cacccagaga cttaaatgcc aaaaccgtat ctctatcccc tactacagcc
     5761 atcttatacg tagacatctc tcaatcgctc ccttatcata tccgaagata tgccgttaat
     5821 ctttcccacc attatgatcc tgagaagttt tatttcgttt tcccgagcag ccaggtaacc
     5881 tatgaccgtt tcagacccaa atggtttgta aaggccgcgt cttgccagtg aaatcaggaa
     5941 attgtctatc aatttttcaa aaagagaagg gctgcccgtt gattcccact ggctgatgcc
     6001 ttcttccaca agttgcttgt acggagtgtt gtcaagagcg tcaattaggc tttgactgct
     6061 ttcaaagtat ttctctttaa agaattcctt tgacaagctt ccgccactta ctaaggcatt
     6121 gtcgagagtt cgaatatctg catccatttt tttaagtctc atcattatct ttatatttgt
     6181 caggtccgaa agagcggtca agtattcttg caaaaatgaa tctcttgaca ccttaacaat
     6241 ttcttctaaa ttatcaaaaa gtgcctggtc taaagcaaaa tcaatttgct gagggtcttt
     6301 ggtcagctca taagcttcca ccgctctatc gtaagctgct tttagctcat caggtatatc
     6361 ggcacctgtt tcctcctgga catatttttt tatctcttca ggaggaatct ctgctagatg
     6421 agacaggttc ccattctcgg tacccgaaat cattgcctta acgattacct ttaaattgtg
     6481 gaagtcatgt ttcatggtga agaatcttat gaactcgtgg ttcgagaaag attccttcaa
     6541 agtcctgatt gtccttatca tgctcttgtc caggactttc tcaaactcgt atatattttc
     6601 catttcggct aaatcactgc cgtactcggt atcgctaagc actttaactg cttcttccgg
     6661 gcccggagca tcgagtattc gttcgatatc gcttctgccg aggagtttgg tttctaaatg
     6721 ttttattcgg gctgaaacat ataggaaatc tgtgtctttc gccatttaat cactcctcga
     6781 atagaatttt ggcaatttct gtttccagtt cttccctttc tatatcaata agggagtcaa
     6841 aggtactgtt tatctccagg tcctgggatt tcagcacgaa accacctatc atattcccat
     6901 aggttttcga aatcctaaga tttccctgct tgcccatgcc cgaaagagta ttgttgattt
     6961 tttgtataaa gtcgggtgtt atcctgtttt tgtcatgttc ggcaataata atctcttcat
     7021 tgccggtaat gacactgctc aaaagcatgt tttcaattaa agtttgatac ttctcaaggg
     7081 gcacgttttt aagggaagtc tttgcccttt cgaaaacttc atcaattatt tgttgttttg
     7141 ccagcagcag ttgctttctg ttttcaagct gggccataga aagaattctg cgcctttttt
     7201 cttcagcagt cttgcccgct ttttccaata tatcatcaag aatatcttta gctttttgct
     7261 catattgttt cacaatgttc tgagcctgtg cattggcatc tgaaataatc ttttcctttt
     7321 cttcccgggc ttcttccaaa atcctttcct ttatctttgc aataccctcc atggtataca
     7381 ctcccttata cgttgatgcc gaacaacatc aatatggttg ccagcagtgc aagtacagca
     7441 taggtttcta ccatggcggc aaaggtaatt cccttggcta cttcttcagg ccgtttggat
     7501 acgatgccta taccggcagc agcaactctg ccctggtaaa tagctgataa gaagcctacg
     7561 atggcaatag gcatgcaaga agcaaatatt aaccaacctt gataaggtgt aatgtcaact
     7621 aaagtcccag ccaggagacc aattttttga atggtcaaaa atcctgctaa aaatccgtaa
     7681 ataccctggg ttccaggaag tgcctgaaga atcaacgctt gaccaaattt accaggatct
     7741 tcagtcacca agccggcagc gctctcaccg acgatcccta ctcctcttgc agatccaatt
     7801 cccggtagac ctacggccaa tgccgcacca aataaagcta atacttgtcc tagtgttagt
     7861 gacatttgta ctcctcctcc aaaaattgtt tactttttat tatttaaatg tcttccaaat
     7921 ccacatagct tgttttaact cttaaggggt caaaagccct gcctccgctg tcgtaaaact
     7981 tgctaaagaa ttcaatgtac tgcagacgac tgctgtgaac gtaagcgcca agtacattta
     8041 tagccaaatt gaacacatgg ccacctacga taatcagtac catcacgata tatcctatga
     8101 tgctacctgc tacagtcttg gccatggtgt ttataacggt tgctatgaca cctgttgtaa
     8161 gacctagggc caatagcctt gagtatgaca ggacatcgct gagatatccg gttatatcgt
     8221 acaaactcaa tacacccgat gtaaacttct tgatgatgcc cttttgggat ctcccctggg
     8281 ttaatatcaa gcccaccgct ccgaatatgg ccatatattt cccaagctgg gcaagttcgg
     8341 gcattacgaa catcaaaaga cctgtcaaaa gtaccagcca caagccctgg tccattaaag
     8401 catctgctac ttttcctgcc ctgatgttct tgtatgcatt taagatgatt cccacatata
     8461 tctggacgac tcccattaca aaggatagta tgagcactga aaggggatta tccaggggat
     8521 ttatccacat tggctttact ctaatcaggt caccgaacca gccaccgaag attgcacccc
     8581 atattaccgt agatatgccg cagaggaata acatggccgt aaatttcttg cccgagccgg
     8641 ccagtttaaa tctgaaaagg gcataaccgg ccagcaggct catgataatg ccatagcccg
     8701 cgtcgctaag catcatcccg aaaaatacaa agaaaaatgg ggccatgtaa agattagggt
     8761 cgagtcctcg cgggtccgga agactgtaca gctcggttat cacctcaaag ggctccacaa
     8821 gcttagcatt ggagagcagc accggcatgt catcttcatc tgttggtgca ctaaattcca
     8881 tgtagaccgt atctgttact tttgaaacag tacttttcac cagttcttcg tgttcttcag
     8941 gaagccaagc ctgcatggaa aaagctttat cggtatcagt cacctttaaa aaggcgcttt
     9001 tcctatcttt ttccacggtg taatagtcgt aaagaatctg aaaatatcgt aaattatcgg
     9061 acaattcttt ggatttttcc tttatggttc ctcgttctac ttcaatgtct tcaattcttt
     9121 tttcagctgc cttaatgagt tccttgggtg ttccctcgaa atttgagaaa tcctggaggt
     9181 tcaccgaaaa ctctttaaac agcttttcca tgttttcttt gtgctctttg tggtatacaa
     9241 caagtgtata cacgtcatcc tggctctcac ccagtgttgc tatttctgcc aataggccct
     9301 cctcagtaat tatcctgtct aattctgccc tggattttct aggcataact gccgcctcaa
     9361 agacagtttt gcgtgtttcc cccagttctt ccagaggaat atccagagca cgccaaggtg
     9421 aaagcatctc tatttgacct attagccttg tttcttccgt ctttagctga gataattgct
     9481 tatccaggtc aaaaactgcc tgtagattgt ctaaaatact actctctttt gaaagaattt
     9541 ctgacagttc atcctttttt atacttggtt taccgtgtat aagaggatta gtggatttaa
     9601 catatggttt taaaaaatca ataccaaaat taagttcagc aagcttagtt tccagctcct
     9661 gcaattctct attgagttcc tggtttacgt aatttttctg cacctcacca gcttgttcaa
     9721 tatcctctac aaaatccagc gtaggctgca cctcgggatt cagtcgcgac ggagtcttgg
     9781 cagtgacctg ttttctggga atcaagcgcc cgtgctcgtc acccaccacg ggtggaacca
     9841 gccattgcca ggcctgctgt acagattcgt cttgcggcat gaatgcgctg gctttatttg
     9901 cgataggcct cagggtccag gcgctgcatc tcggattcga tccaggcttg cacctcggcc
     9961 atcagctcat cgtgcttgcg gcccaccgaa gaaatgggct tgccgatgga aacatccacc
    10021 acgccgggct ttttgataaa ggctttacgc ggccagcatt tggcggaggt cacagccaca
    10081 ggcaccacag atgcgccagt catg
//
LOCUS       DAONUO010000029        10097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5114_length_10097_cov_3.976399, whole genome shotgun sequence.
ACCESSION   DAONUO010000029 DAONUO010000000
VERSION     DAONUO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5114_length_10097_cov_3.976399"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PK768_01840"
     CDS             complement(<1..615)
                     /locus_tag="PK768_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HQA59743.1"
                     /translation="MKKVIVLFLIIAITILIIPCYGYSAQDLQKKAIIFIVDNVNYDD
                     LAKYGQANIRYLLENGSIGLMNTNSGGAYNSPNAYATIGAGTYAVSSTVGTYAGGYDD
                     LYYGDEIHKVYKRNTGGEMDEENIANIDILGLKLLNERLNRPVKIGLLGSLLNEQGYK
                     TAIIGTEATNLDDISNRATLITMNGEGITNYGKVDGSLLTRDFMS"
     gene            776..1894
                     /gene="steA"
                     /locus_tag="PK768_01845"
     CDS             776..1894
                     /gene="steA"
                     /locus_tag="PK768_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HQA59744.1"
                     /translation="MTIIGTAKIDHKTKNLINRLEPGDIAVINHEDIDEVAALGLTAS
                     KVKAVINAAKSITGRYPNPGPGIILEANIPIIDDVGQQIMSCKEGSKVTIRDSGEIIV
                     DDKLVAMGEFLTPEKVKEQMEAAKKNMEVSLDKFIDNTLDYAKKEKYLIMGGVEIPET
                     DTVIKGRHVLIVVRGNNYKEDLAAIKTYIDEVKPVLIGVDGGADALLEYGYKPDIVIG
                     DMDSVSDEALRMCRDIIVHAYPNGKAPGLSRVKELDLDAKTFVAPGTSEDIAMLLAYE
                     KGADLIVAVGTHSSMIDFLEKGRKGMGSTFLVRLKIGSSLVDAKGVSKLYNQKLKPSY
                     MMGLFAAAMVPIIIISTISPPIKHALKLLELRLKLLLP"
     gene            1908..2819
                     /locus_tag="PK768_01850"
     CDS             1908..2819
                     /locus_tag="PK768_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HQA59745.1"
                     /translation="MLINIKYLVITVISIFLALGIGILIGIQIDSQDIILEQQEITVQ
                     RMENKLDELNRINLDLQNELGELTTINDIHENYIKNVFPDYIKNKLLGLHIIILETTD
                     EYTYASMRQALKTAGAQVTSVTIISDKILNMSESEIAQLKEYFGIADNVNIIQAILKR
                     IAETVGGKYEPDDIAFLIERGIIYVNGDLQQPADYVVLTGGSSKEDNKHEIIDIPLIK
                     ELKKLSLPLAGVETTNVEISYIDIYKKQQLSTVDNVDTAIGQTSLVLVIKGKEGHYGV
                     KKSAQALMPYLNEEEISGEGNDTDTRF"
     gene            2788..3483
                     /locus_tag="PK768_01855"
     CDS             2788..3483
                     /locus_tag="PK768_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQA59746.1"
                     /translation="MKVTILIPAFNEEKNIAHTIKGLENFRDTFCQKNQLDLGIIVID
                     DGSKDKTGKKAIDAGVEVIQLKQNKGKGNALREGLKQADGDIIVFLDADLRDSSEEAY
                     KLVTPILNGEADVTIARFKPPGKKKGLGFVKALAYHGVKFFTGHGVTSALSGQRAFKK
                     EVLKSIGTIPDGFGIEVGMLIDILRKGFVVKEVDVNMQHDVTGRDFRGFMHRGKQFWH
                     ILKVLISKLFASD"
     gene            3952..4782
                     /locus_tag="PK768_01860"
     CDS             3952..4782
                     /locus_tag="PK768_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA59747.1"
                     /translation="MSRLYVFIIFIAAAIFSNALTPAFIDLFKSSGRLVKNYKGEMIP
                     QGIGIMFPIYYLLWYAFLKLFNESFMDNALLILFAITTVCFIGFIDDMLGTRDVLGLK
                     GHFKSLLKGRLTTGALKAIVGLLLAFVLSFPLATTFIEVIVNTLIIALFTNFFNLLDL
                     RPGRAIKVYIIIFLALLAALNLTEKTDILLTFVPLLGFVVGYLPYDLKASSMMGDAGS
                     NVLGISVGLLVVLSLSWHIRIAVLAFLIGIHFFTEKYSLTKLIEKNSLLNYLDNLGRS
                     "
     gene            4789..5868
                     /locus_tag="PK768_01865"
     CDS             4789..5868
                     /locus_tag="PK768_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HQA59748.1"
                     /translation="MINYAKGRVKEIICKRRNCTEIAVVIDNEECKAVNYDSITGTVK
                     PGDRVLLNTTAQDLKLGSGGYHFVVSVNDGDTVFPTFEKTGHIMKLRYTPLQFAVLSA
                     DEQQSPYHKVFDDFESLKGMPVIIGELHSMLAPIVFNIKQNRPELRVSYIMTDGGALP
                     LSFSRTVAYLREKELIQGTVTFGQAFGGDFETVNIYTALITAYEILKSDIAIVTMGPG
                     ITGTGTKYGFSGIEQGPIIDAVNTLGGRPIFIPRISFADSRKRHCGISHHSVTVLKEI
                     AKTRAEVVIPLMEVDKMDLIKKLIVKNELDKNHKIVYIEERDKILESLLHFDFKLSTM
                     GRGLDQEEEFFLTAGAAGFYASSIL"
     gene            complement(5992..6873)
                     /gene="ilvE"
                     /locus_tag="PK768_01870"
     CDS             complement(5992..6873)
                     /gene="ilvE"
                     /locus_tag="PK768_01870"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HQA59749.1"
                     /translation="MSIQVYVNGEFFPKEEAKISVFDHGFLYGDGVFEGIRAYGGRVF
                     KLKEHIDRLYDGARGIMLDIPLTKEEMTEVVLETLRRNQLKDAYIRLVVSRGKGDLGL
                     DPRKCQKPTIICIADKIVLYPEELYTEGMEIITAATRRNRPEGVNAQMKSLNYLNNIM
                     AKIEANLAGVPEALMLNTEDYVAECTGDNIFIVKNGVLITPPPFVGILVGITRNTIIE
                     LAKQMGIKVEEKVFTRYEVFTADECFLSGTAAEAIPVVKVDGRPIADGKPGPVTKKII
                     EAFKELIKTEGTEIYKN"
     gene            complement(6927..8606)
                     /gene="ilvB"
                     /locus_tag="PK768_01875"
     CDS             complement(6927..8606)
                     /gene="ilvB"
                     /locus_tag="PK768_01875"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQA59750.1"
                     /translation="MTGAELLLKTLTELGVDTIFGYPGGSALPIYDALYSNKKIRHIR
                     TVHEQGAAHAADGYARTSGKVGVVLSTSGPGATNLVTGIANAYMDSIPMVAFTGQVSL
                     DLLGRDSFQEVDITGVTLPITKHNFLIRDKRDIVKKVKEAFFIAKNGRPGPVLVDLPK
                     DVMMQEIIEDEIRKDFNGFIDTFSSKLNANKKFGNLTEKAAEMIMQAKRPVIYAGGGI
                     ISSDACDELLQLAERINAPVTTTLMGITGFPSDSPLSLGMLGMHGTAYANMAVTHCDL
                     LLALGARFDDRVTGNVARFAPKAKIIHVDIDPAELGKNVNIDLGIKGDVKHFLKELLS
                     ILPSKQHDEWLKTVIDWKKSYPLKYCDKGLKPQYVVEEICRQTGGNAVIATEVGQHQM
                     WAAQYYKLKEKRTFVSSGGLGTMGFGLPAALGAKLGRPEKPVFNIAGDGSFEMNCHEL
                     ITAARYNIPVITVIFNNRSLGMVRQWQELFFSERYSHSLMDGVDADYVKLAEACGVKG
                     YCVSEKSQLSKAIEEAMAIQAPAVIDVKIDPKENVMPMVPAGKAIDEMLGI"
     misc_binding    complement(8672..8934)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9014..>10097)
                     /gene="leuB"
                     /locus_tag="PK768_01880"
     CDS             complement(9014..>10097)
                     /gene="leuB"
                     /locus_tag="PK768_01880"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQA59751.1"
                     /translation="EGGKIMHKVALLPGDGIGPEIIDEAVKVLKAVERRFGLEFEFME
                     GLVGGASIDTCGLPLSEEVLELCMSSDAILFGAVGGPKWDDLPKEKRPEQAILKLRKR
                     LGLYANLRPLVFFGALIDASPVKREILGDSLDIMIVRELTGGLYFGKKGTLKTQETIR
                     AFDTMEYTEEQIKRVAKIAFEIARNRKGNLVSVDKANVLETSRLWRSIVSEMAKDFPD
                     LKVSHMYVDNCAMQLVLNPKQFDVILTENTFGDILSDEASVLTGSIGILPSASLGESK
                     PFLYEPIHGSAPDIAGTGKANPIACILSAAMMLEYSFGERAAAEAIKNAVKRALDEGY
                     RTADIQLGAKYTVSTSEMGDVIAGYL"
BASE COUNT         2824 a         2252 c         2084 g         2937 t
ORIGIN      
        1 gctcatgaag tcccttgtta aaaggctgcc gtctactttt ccgtagttcg ttatgccctc
       61 gccgttcatt gtgattaatg ttgccctgtt gctgatatcg tctaaattag ttgcctcagt
      121 tcctattatt gcggttttat agccttgttc gttaagcagg gagcctaaaa ggccgatttt
      181 aaccgggcga tttaagcgct catttaaaag ctttaggcct aatatgtcta tgttggcaat
      241 attctcctca tccatctctc cgcccgtatt cctcttatag accttatgta tttcgtcacc
      301 gtagtatagg tcatcatatc ctcccgcata tgttccaact gttgaactta ccgcatatgt
      361 tcccgctcca atagtggcat aagcattagg actgttatat gcaccgccgg aattcgtgtt
      421 catgaggccg atggagccgt tttccagcaa ataccttatg tttgcctggc cgtattttgc
      481 caggtcatcg taatttacat tgtcaactat gaatattatc gccttttttt gcaaatcctg
      541 agcagaatat ccgtaacagg gtataataag aatggttatt gcaataatga ggaataaaac
      601 gatgactttt ttcataagga taccaccagt taataccact attataacgc tattatatca
      661 atctaattca gcccttgcaa taaaaatgca aattggaggg catgttgagc aagttgtatt
      721 gttgtgctaa attataatca ggactttcag tagtctttca cacgaggtgt ttcaaatgac
      781 aattatcgga actgcaaaaa tagatcataa aactaaaaac cttatcaatc gactggaacc
      841 cggagatatt gcagtaatca atcacgaaga tattgatgaa gtggcagccc tgggcttgac
      901 ggctagcaaa gtaaaagccg tgataaatgc cgcaaaatcg attaccggcc gctatccgaa
      961 cccgggtccc ggcatcattt tagaggcaaa catccccata atcgacgacg tgggacagca
     1021 aatcatgtcc tgcaaagaag gctcaaaagt tacaataagg gacagcgggg aaatcattgt
     1081 tgatgacaaa ttagtagcaa tgggtgaatt tcttacacct gaaaaggtaa aagagcaaat
     1141 ggaagctgcc aaaaaaaata tggaagtttc actggataag tttatagata atacattaga
     1201 ttatgccaag aaagaaaagt acctgataat gggcggtgtg gagataccgg aaaccgatac
     1261 cgttataaag ggcaggcatg tcttaattgt agtgcggggc aacaactata aagaagattt
     1321 ggccgcaatt aaaacctata tcgatgaggt gaaacccgtg ctgataggcg tagatggcgg
     1381 tgccgacgct ttgctggagt acggatataa acccgatatc gtcatcggcg acatggatag
     1441 cgtaagcgat gaggcactgc gaatgtgtag ggatataatc gtccacgcat accccaacgg
     1501 caaggcgccg ggtctttcca gggtaaagga gctggacctt gacgctaaaa ctttcgttgc
     1561 tccgggcacc agcgaagata ttgccatgct cctggcatac gaaaagggcg ctgatttgat
     1621 tgtagcggtg gggacccact caagcatgat tgattttttg gaaaaaggtc gcaaaggtat
     1681 gggaagcaca tttttagtca gactgaaaat tggctccagc ctggttgatg ccaaaggggt
     1741 aagcaagctg tacaatcaaa aattaaaacc ttcttatatg atgggcctgt ttgccgcagc
     1801 catggtaccc attattataa tttccaccat atcacctccc attaaacatg ccctgaaact
     1861 cttggaactg cgactaaaac tgcttttgcc ttaaaggagc tggaacgatg ctgataaaca
     1921 taaagtacct ggtcataact gtaatttcta tatttttggc acttggaata ggaatcctta
     1981 taggaattca aatagattcc caggatatca ttttagaaca gcaggaaatc acagtccaga
     2041 gaatggagaa caagctggat gagctcaacc ggatcaattt agacctacaa aatgagcttg
     2101 gcgaactgac gactattaac gatatacacg aaaactatat taaaaatgtt tttcctgact
     2161 atataaagaa caaattgtta ggcctgcaca taataatttt agaaacaacg gatgaatata
     2221 catatgccag tatgagacag gccttgaaaa ccgccggtgc ccaggtcacc tcggttacaa
     2281 tcatcagtga caaaatcctc aatatgagcg aaagtgaaat cgctcagtta aaggagtact
     2341 tcggcattgc agataatgta aatataatcc aggcaatcct caaaagaatt gcagaaaccg
     2401 ttggcggcaa gtacgagcca gacgacattg cctttttgat agaaagagga atcatttatg
     2461 taaacggcga cctgcaacag cctgctgact acgtcgtttt aaccggaggc agcagtaagg
     2521 aagacaacaa gcatgaaatt atagatattc cgcttataaa ggagctcaaa aagttgtccc
     2581 tgcccctggc cggagtagaa actaccaatg ttgaaatttc ctatattgac atctataaaa
     2641 aacagcaact atctacggta gacaatgtag atacggctat aggccagact tccttggttc
     2701 ttgtcataaa aggaaaagaa ggtcattacg gtgtaaaaaa gagcgcccaa gccctgatgc
     2761 cctatctaaa tgaggaggaa atttcaggtg aaggtaacga tactgatacc cgcttttaat
     2821 gaagaaaaaa atatagcaca caccataaaa ggccttgaaa attttcgtga cactttttgc
     2881 cagaaaaacc agctggattt gggcattatc gtaatcgatg acggctcaaa agataaaacc
     2941 ggcaaaaaag caattgatgc cggagtggaa gtaattcagc ttaaacagaa caagggaaag
     3001 ggcaatgccc tccgtgaagg cctgaagcaa gccgatggtg acataatagt tttcctggat
     3061 gcagaccttc gggatagttc cgaggaagct tacaaactgg tcactccaat tttaaatggt
     3121 gaagcagatg taacaatcgc cagattcaag ccgccgggca agaaaaaagg ccttggtttc
     3181 gtcaaagccc ttgcatatca cggtgtgaag ttttttaccg gtcatggtgt aacaagtgcc
     3241 ctgtcaggcc agagggcctt taaaaaagaa gttctcaaat caataggcac aatccctgat
     3301 ggttttggaa ttgaagttgg aatgctgatt gacatactga gaaagggctt tgtggtcaag
     3361 gaagtagatg taaacatgca gcatgacgtt accggcaggg attttagagg atttatgcac
     3421 cggggaaagc agttctggca catattgaag gtgcttattt caaagctttt tgcttcagat
     3481 taatcaatag gcggctttga tggaaaagct gtgttgcgtg acgaatgtca taaagtgata
     3541 acagcgtaat aaaaacattt gtatctttat ctaaaatagg tttgatttca atggaatata
     3601 attaacttct atgatttgtg gggagctcgt caggtaatac cagcgagtga atatttatta
     3661 ttttgttaat ggttcttgtc gagatttttt caaaagccgt taaaaagcat ttcgcggggt
     3721 agcttcgatg ataatctttt tcttgtcgat ttagctgttc attcaacgtg ttaaagtttc
     3781 aatccagact gagcttaaaa gatttaggct tttgaaaaat tgagacctag aaccatcaaa
     3841 aaaaaagaaa cttgaacgag cggtattacc tgacgagcgt cataaagata agacagcaac
     3901 ataatacttg tcttatagct aaattagttt atatttgtgg aggaaaatcc attgtcaagg
     3961 ctttatgttt ttataatctt tatcgctgca gccatcttca gcaatgcttt aactccagcg
     4021 tttatagatc tattcaagag cagtggcagg cttgtaaaga attataaagg cgaaatgatc
     4081 ccccagggaa tcggcataat gtttccgata tattatctgc tttggtatgc atttcttaaa
     4141 ttgttcaatg aaagctttat ggacaatgct ctcttgattc tatttgctat tacgacagtg
     4201 tgctttatcg gctttattga tgacatgctg ggaactcgag atgtgctcgg acttaaaggc
     4261 cattttaaaa gcctgctcaa aggccgtctt accacgggag ctttaaaggc cattgtcggg
     4321 ctgctgctag cttttgtcct cagtttccct cttgcgacga cttttattga agtcatagta
     4381 aacacactaa ttatagcact gtttactaac ttctttaatc tgctggattt aaggccaggc
     4441 cgggcaatta aagtttacat aatcatcttt cttgcccttt tagctgcttt gaacctaacg
     4501 gaaaaaaccg acatactgct tacctttgtc cctcttttgg gatttgttgt cggctatttg
     4561 ccatacgatt taaaagcaag ttccatgatg ggggatgcag gttctaacgt gcttgggata
     4621 tcagtgggat tattagttgt gctttccctc agctggcata taaggattgc ggttttggca
     4681 tttttaatag gcatacattt ctttacggag aagtactcgc tgaccaaatt gatagagaaa
     4741 aattctctct taaattatct tgacaatctg ggaaggtcgt gaactatcat gatcaattat
     4801 gcaaaaggca gagtcaaaga aatcatatgc aaaagacgga attgtaccga aattgctgtg
     4861 gtaatagata atgaggaatg taaagctgta aactatgaca gtataacggg aaccgtaaag
     4921 cccggcgatc gggtgctttt aaatacaacg gcccaggact tgaaactcgg cagcggcggc
     4981 tatcacttcg tcgtttccgt gaatgatgga gatactgtat ttcctacttt tgaaaaaaca
     5041 gggcacataa tgaaactccg atacactccc ctacagtttg ccgttttgag cgccgatgag
     5101 cagcaaagcc cttatcataa agtatttgac gactttgaaa gtctaaaagg tatgcccgtt
     5161 ataatcggag aactccacag catgcttgcc ccgatagttt tcaacataaa gcaaaatcgg
     5221 cccgagctgc gggtgtcata cataatgacc gatggcggcg cactgccttt aagctttagc
     5281 cgaacggtgg cttacttaag ggaaaaggaa ttaattcaag gcaccgtaac cttcggacaa
     5341 gcctttggag gagattttga aaccgtcaat atttacacgg ccttgataac ggcttacgaa
     5401 attttaaaat cagacattgc aatagttact atggggcccg gcataaccgg aacgggcaca
     5461 aaatacgggt tttccggcat cgaacagggg ccgataatag atgcggtaaa caccttaggc
     5521 ggcaggccta tattcattcc caggataagc tttgcagaca gcagaaaaag acactgtggc
     5581 ataagtcacc acagtgtcac cgttctcaaa gaaatcgcca aaacccgcgc tgaagtcgtc
     5641 attcccctta tggaagtaga caagatggat ttgataaaaa aactgattgt gaaaaatgaa
     5701 ctcgataaaa atcacaagat tgtctatatt gaggagaggg acaaaatcct ggaaagcctc
     5761 ttacattttg actttaaatt gtcaaccatg ggaagagggc ttgaccagga agaggagttt
     5821 ttcctaactg caggtgcagc gggattttat gcgtccagca tcctttaaga attttggccc
     5881 ttcccacact actttcctaa aaagtttact gacaatttgc tgctaatact ataaccggtt
     5941 caaacgctgc actacttcct tttttgcttc taaacttttg tcaaagaccc tttagttttt
     6001 gtatatttca gttccttcgg tcttaatcaa ttccttaaag gcttcaatga ttttcttcgt
     6061 cactggcccg ggcttgccat cggctatagg ccggccatct actttcacca caggtattgc
     6121 ctcggcagca gtgccggaca ggaagcactc gtctgctgta aagacttcat accttgtaaa
     6181 gaccttttct tccactttta ttcccatttg ttttgccagt tctatgatgg tatttctagt
     6241 tatacccact aatattccca caaagggcgg aggggtaatt aagacaccgt ttttcactat
     6301 aaaaatatta tcgcccgtgc attcagctac gtaatcctct gtgttgagca tcaatgcttc
     6361 gggaacccct gcaaggtttg cctctatctt agccataatg ttgttgaggt aatttaggga
     6421 cttcatctgg gcatttacac cttctggcct gttccttctg gtagctgccg ttatgatttc
     6481 cattccttct gtgtagagtt cttccgggta cagcacgatt ttatccgcga tacatataat
     6541 agttggtttt tgacactttc tcgggtcaag acccaagtct ccttttcctc gcgatacaac
     6601 caaccttata taggcatctt tgagctgatt tcttctaagg gtttctagta ccacttctgt
     6661 catttcttct tttgtaagag ggatgtcgag cataattccc ctcgctccgt cgtatagcct
     6721 gtctatgtgt tcctttagct taaaaactct gccaccgtat gcccttatcc cctcaaatac
     6781 tccatccccg tacaaaaaac cgtggtcgaa aactgaaatt ttagcctctt cttttgggaa
     6841 aaattcaccg ttaacatata cctggatact catcctttaa tcttcctcct ttagaaataa
     6901 atatatatgt tagcagataa tatgctttat atgcccagca tctcatcaat ggctttaccc
     6961 gccgggacca tgggcattac attttctttg ggatctattt ttacatcaat tactgcaggt
     7021 gcctgaatgg ccatggcttc ttctattgct tttgacagtt ggctcttttc tgaaacgcag
     7081 tatcccttta ctccgcaagc ctcggccagc tttacgtagt ccgcatctac cccatccatc
     7141 agcgaatggg agtacctttc gctaaagaaa agttcctgcc actgcctgac cattccgaga
     7201 ctccgattgt taaatattac tgttatgacc gggatgttgt acctggcggc agttatcagc
     7261 tcatggcagt tcatctcgaa gctgccatct ccggcaatgt tgaacacggg tttttccggt
     7321 cggcccaatt tggctcctaa ggctgcaggc agaccaaaac ccattgtgcc caagcctccc
     7381 gaggatacaa aggtcctctt ttctttgagt ttgtaatact gtgccgccca catttgatgc
     7441 tggccaactt ccgttgcaat tacggcattg ccacctgttt gacggcagat ttcttctact
     7501 acatattgtg gttttaaacc cttatcgcaa tacttcaggg gataagactt tttccagtct
     7561 attacggttt ttaaccactc atcatgctgt tttgatggca gtatgcttaa caattccttt
     7621 aagaaatgct ttacatcacc ttttatgcct aaatctatat taacattttt cccaagttct
     7681 gcaggatcta tatcaacatg gatgattttg gctttcggcg caaacctggc tacatttccc
     7741 gtaaccctgt catcaaacct ggcgcccaat gccagcagca aatcgcagtg ggtcaccgcc
     7801 atatttgcat aggcggtccc atgcatcccg agcattccaa ggcttagtgg actatctgaa
     7861 ggaaagccgg taatacccat aagcgtcgtc gttaccggtg cgtttatcct ctctgcaagc
     7921 tgcaataact catcacaggc atcggagctt attatcccgc cacctgcata aataaccggc
     7981 ctttttgcct gcatgatcat ttctgcggct ttctcggtca gatttccgaa ctttttgttt
     8041 gcgttcagct tggaagaaaa tgtatcgata aagccgttaa aatcctttct gatctcatcc
     8101 tcaattattt cctgcatcat aacgtcttta ggcaaatcca caaggaccgg ccccggcctt
     8161 ccgtttttgg ctatgaaaaa agcttccttt acttttttta cgatatctct tttgtcccga
     8221 ataagaaaat tgtgctttgt aattgggagg gttacgcctg ttatgtctac ctcctggaag
     8281 gagtccctgc ccagcaagtc cagggagact tggccggtaa atgccaccat gggtatcgaa
     8341 tccatgtagg cattggctat tcccgtaacc aagtttgtag ctccaggccc ggaagtgctt
     8401 aacacaaccc ctacttttcc tgaagtcctc gcataaccgt cggcagcatg ggctgctccc
     8461 tgctcgtgta ccgttctgat atgccttatt ttcttgttgc tgtacaaagc atcatatata
     8521 ggtaaggctg aaccaccggg atatccgaaa atagtgtcta cacccaattc agtcaatgtt
     8581 tttagtagta attctgctcc agtcatcatt tcacctcttt ttttcaaaat taattatagg
     8641 tataaaaaaa gattcttcac ctaaccttat ataaaggggc gaagaatctc gcgttaccac
     8701 cctatttcgt ttatacctcg cggtataaac ctcagtaggt acagggcatt cacccgatac
     8761 ccggcctttt taacggaggc caatttccac attgggattc cggccaggat tactacgatt
     8821 tcaccctggc agctccgaga cgattttcgt tgactttttt aaacgtcggc tttcacctga
     8881 cccgactctc tgtggtttaa aaaacgccac tactcttctc ttcatagctt ttattaacag
     8941 cctttttaaa tttatttaga gttgattata tcatttattg cataaactta caagagttta
     9001 ttttgatttt ttgtcaaagg tatcctgcaa taacgtcgcc catctcagat gtagacactg
     9061 tatatttcgc accgagctgt atatcggcag tcctgtaccc ctcatcgaga gctcttttta
     9121 ctgcattttt tatggcttcc gcggctgctc tttcaccgaa agaatactcc agcatcatcg
     9181 cagctgacag tatgcaagct atcggatttg ccttgccggt gccggcaatg tccggcgccg
     9241 aaccgtgtat gggttcatac aaaaacggtt ttgactcacc taggcttgcc gatggcaaaa
     9301 tgcctataga acctgtcagc accgacgcct catcgctcaa tatatcccca aaggtgtttt
     9361 ccgtcaatat gacatcgaac tgctttgggt tcaagaccag ctgcatggca caattgtcta
     9421 catacatgtg actgaccttc aggtcaggga agtcttttgc catttcggaa actatgcttc
     9481 tccagagcct ggaagtctcc aagacatttg ccttatctac agagacgaga ttccccttcc
     9541 tgtttctggc aatttcaaag gcaattttgg ctaccctctt aatctgctct tcggtatatt
     9601 ccatagtgtc aaatgcccgt atggtttctt gagtctttag cgtccctttt tttccaaaat
     9661 ataggccgcc tgtcaattcc cgaacaatca ttatatcaag gctatcgccc aaaatctccc
     9721 tctttacggg ggaagcatcg attaatgcac caaaaaacac aagcggcctt aaattggcat
     9781 aaaggccgag cctttttctc agctttaaaa ttgcctgctc cggccgcttt tccttgggca
     9841 gatcatccca tttcggcccc cctacggctc caaacaaaat ggcatcactg ctcatgcaaa
     9901 gttccagcac ctcttcgctg aggggaaggc cgcaggtatc tatagaggca ccacccacca
     9961 agccttccat aaattcaaat tcaagaccaa agcgcctttc taccgccttt aatactttaa
    10021 ccgcttcatc gatgatttcg ggaccgatgc cgtctccagg cagaagtgct actttatgca
    10081 ttatttttcc accttct
//
LOCUS       DAONUO010000030         9974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5214_length_9974_cov_3.916524, whole genome shotgun sequence.
ACCESSION   DAONUO010000030 DAONUO010000000
VERSION     DAONUO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5214_length_9974_cov_3.916524"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PK768_01885"
     CDS             <1..1058
                     /locus_tag="PK768_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59752.1"
                     /translation="GKLYIATRDELEENPVPLLYSSEDPEFYPWEPVITNVQGFDPDK
                     NPRGSIWNMAVFNNRIYISTSYEDRIQVWRTNDEEPRLNDWTLVVDGFENPLNTSTLA
                     MGVFKNHLYVSGTKPLPLAWLIPFGFDLIRIDKDDNWETIVGGNPLILFDTIIDKDSD
                     CLSNLGSGFNNPFNVYGWQIQEYKDKLLISTFDDSNNMEVILTTLLENRAALENLIGE
                     TATNILIAAYRAVVEILTAIRYPRGFDLYISEDGVNFSSVFLDGLGNPHNYGGRILFV
                     DSKNDLYLGTANPFDGCEVWRTDNIPTEALQPCGDNHYEGLLKIKEMLSENFKVINDN
                     MPVILELMSKNAYLKSM"
     gene            1300..2613
                     /locus_tag="PK768_01890"
     CDS             1300..2613
                     /locus_tag="PK768_01890"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HQA59753.1"
                     /translation="MKKDIKILLLVITVLLFTFLLQGCSNSNNSTDHSASENNLGTPS
                     EDPSTAVDESLPLNISFKVYSETVKDDDGSDLVSFRFSYPYIENPDGTEGIEEINNYY
                     QRQLEHFISNVVPEGKKAALDAKKAAEEGSFDFFGLFFERESNIYYDENNLLSVLNIS
                     YEYTGGVHPMSYWSSETFDVRTGEMLTLSDIFGLSDEEALEKVYQLVISQIEEAKDKE
                     GIYFDNYRENVKSYSETDFLLGPNGIIFYYQLYTIAPYAAGIPTFELPYDETELAIGI
                     LPAKPNDLEREVCLHAGWLIEANKDIFYNIFGLSMLPLELPEEIPAEQSLFPVKDKRF
                     VTFSELDSYIRGVYLKSEADSLLNSGRYVDKDGRLHGDISKDTGMGYYIDWNNYRYKV
                     TDIKENSANITIYVVDDSPAGREETTIEVKMLKENDRWLLERMFY"
     gene            complement(2836..>6924)
                     /locus_tag="PK768_01895"
     CDS             complement(2836..>6924)
                     /locus_tag="PK768_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA59754.1"
                     /translation="MLTGRLPLKADAAAEWAELHATKVPERPDTIDAKIPSVLSDIVM
                     KLLAKSARDRYQTAYGLLKDLLECKRQWLKSGKIEPFKLGMHDISTRFKLPTKLIGRE
                     RETAVLKQAFDRARSGSAELVFVTGTAGIGKTMLINETVKALVADEGYYGYGKYDQLR
                     QNVPYAAISSALGIVIRQLMAESKEKLEEWKKDILCSLGNAGALITEIVPDLEMIIGR
                     QPAVEIPQSKEAINRIMMVFENFLKVFSNRNTPLVMFLDDLQWADPASLRLLEFLYKD
                     TKLKYLLIIAAYRDNEIDANHPIQNIIDKKEKTEIPVTQIKLFPFDIKEVTGFLQETL
                     HSRREKTEKLAKILYRKTYGNPFFLSQMLVSICNQKLITFNIKKGRWEWNEESINSFP
                     IPDDVLVLILSRLNNLTKAQLDILKLASCIGNSFDTKTLSMVSNRREAEIAALLIPAI
                     MEGLVLQLIEGDHKPLSTGTEVQNTTYEFLHDRVRQAVYSLFTESEKKTAHLKVGRLI
                     LQLGMGEDLNDKILAAMDHMNRAIDLITDQQECLKLAEYNLLAGRKAKASAAYDTAVN
                     CIKAGIKLLPVNAWETYYDLCFDLYVEYAQNEFLIGNTSEAKELFGLIIRRAKSELDK
                     ADVLGLQILLYTGSGEYAKAVEIGIKALKGLGMVIPANPSTKDLLKELLWYKLFMSRR
                     SIEDLYKLPEMKDPVQRKVAELLIKYILSTSTGYPNLHSFAIIKAGNHTLRYGNTEMA
                     PIGYVGYSIVEGVIFANYAAGDRLGKMAIELTERYDKTYPKCIVYFTFGAIVWHWSHH
                     AKDGIEYLQKAVKYAVEAGDVLINGYSRTVLLENRYIIGTPLIDILDEINNCKNYMQK
                     VKHDNLNLNVGIYSRVVSILTSDIGFHNGKADEDKSVFRDTRDKASLGAYYYTQMQIN
                     YFAGNFREALAEAEKVKDCTASIQGFLLSAECNFYQSLIITAIYEDIPASQRRRLSRI
                     LRDNQKQMKKWAISCPENFLHKFQLVEAERMRIRRNKRQAEELYEKAIQAAYESGYQQ
                     NEAIACELAAKFYIAEGKQRVAKIYMNDALKLYSQWGASAKVKEIRQRYPELLDDILT
                     DNEQLEKDGERDIKNEILRTLITDKETASSLELSILQKTIRDISGEFKSNPESILEAA
                     MKSVFATKGFLIIEKVDELFIEAEIDHQSNSTTVRKPIPLKQCDGKLSKGIVRYVANT
                     RETVIINNVYQHGIFTKDPYLDRTGDVSIACLPLYLDGIPAGVLYLENKLIANVFTEE
                     RLKLLELLIGQTSYAKALEEFLYSIKSREDMSCEAPVEKLTDRELEILKLIAEGLTNN
                     EIAKRLHISNNTVKTHIKNIYEKLHVNRRVQVVKRAKELSIL"
     assembly_gap    6925..6934
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6935..7523)
                     /locus_tag="PK768_01900"
     CDS             complement(<6935..7523)
                     /locus_tag="PK768_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQA59755.1"
                     /translation="MLNLKDYDIQEKVYENGSKILYRGYSSGNGKAVIIKVLKEESTS
                     SADVSRLYYEYEITRKLSTKSIMKPIGLEKAGNSLALIIECDGEISLREYIEGLPLNV
                     ESFLDISIKLTQSLGDIHYCGIVHRNITPDNIFIDPKIKEVRITDFSYAVLYDTDSSK
                     ITIKTHFVDNIEYMSPEQSGQIGGTDFSSDYYSLGV"
     gene            complement(7667..8275)
                     /locus_tag="PK768_01905"
     CDS             complement(7667..8275)
                     /locus_tag="PK768_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HQA59756.1"
                     /translation="MKPYFQLGLMPLIGAIIGWGTNMLAIKLIFRPLNPVKIPILGIQ
                     IQGLIPKRRFDISRSIGEVLEHEIISPEEIVDSLTNKNTKAEIIKYIKNLIANRVSEK
                     LPGYIPLGFRDSISNYIGDMIDRNGAEIFDEMKTVITEKAKDQLDLKQLVENKINSLD
                     IEELEDLIIRLSRQELRQIEVLGGIIGFFIGTIQAAISYYLM"
     gene            complement(8336..9244)
                     /gene="ytxC"
                     /locus_tag="PK768_01910"
     CDS             complement(8336..9244)
                     /gene="ytxC"
                     /locus_tag="PK768_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HQA59757.1"
                     /translation="MRLLTIGSIEPIAGFEDRLKLEFDLLKEDGFEITYKKIAKDNIS
                     FYHCYVDDELLLMENNFINIKQRFINCIANALSDVIINYWEPELIRRIIRENYFYFNK
                     TEQGMIFDFAYSILNYNEISGKRDISYQIKRKNYVLHKIQEFLSTSGLIILDGFVNFR
                     LKEYMRQLEDSVDIAIDEYLMDKEYNEFIKLLRHFVDLQEPKRDLVNVVFSNHKIILM
                     DENLNYIERDSSLDLIAKENPDVNMDDITVSTLINIAPRRITIHGFNGNEKREVAHAL
                     YSIFEGRVRFCNHCELCAKSKSLTKK"
     gene            9420..9584
                     /locus_tag="PK768_01915"
     CDS             9420..9584
                     /locus_tag="PK768_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59758.1"
                     /translation="MNGKTTSWIVTGVGAVVTAVGYMTGGMIGAGITGFGLAHVVLGI
                     LDMFRPSVRN"
     gene            complement(9737..9811)
                     /locus_tag="PK768_01920"
     tRNA            complement(9737..9811)
                     /locus_tag="PK768_01920"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9777..9779),aa:Val,seq:gac)
     gene            complement(9818..9906)
                     /locus_tag="PK768_01925"
     tRNA            complement(9818..9906)
                     /locus_tag="PK768_01925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9870..9872),aa:Leu,seq:taa)
BASE COUNT         2882 a         1992 c         1830 g         3260 t
ORIGIN      
        1 gaggcaaatt atatattgca actcgagacg aactcgagga aaacccagtg ccgctacttt
       61 atagcagtga ggatcccgaa ttttatccgt gggaaccggt tattactaac gttcagggct
      121 ttgatccgga taaaaacccc aggggttcca tatggaacat ggcagtgttt aacaatagga
      181 tatatatatc tacgagctac gaagatagga ttcaagtctg gagaacaaat gacgaagagc
      241 cgcggttaaa tgactggacc cttgttgttg atggattcga aaatccgtta aatacttcta
      301 cccttgcaat gggggtgttt aaaaaccatc tctatgtaag cggaacaaaa ccactgccat
      361 tagcttggtt aattccattt ggatttgacc tgataagaat tgataaagat gacaattggg
      421 aaacgatagt cggaggaaac cccttgattc tatttgacac gataatagat aaggatagcg
      481 attgtctttc gaatcttggg tcgggattta acaatccctt caatgtatac gggtggcaga
      541 ttcaggaata taaggataaa ctcttgatta gcacctttga tgattccaat aatatggaag
      601 tgatactgac aaccctgttg gaaaataggg ctgctttaga aaaccttata ggtgaaactg
      661 caacaaatat attaattgcg gcctacagag cagttgttga aatattaaca gccattagat
      721 atccaagggg ttttgacctg tatatatctg aggatggtgt taatttcagt tctgtatttc
      781 ttgatggtct tggcaaccca cacaactacg gtggcagaat cctctttgta gacagtaaaa
      841 acgatttata ccttggaaca gcaaatccct ttgatggctg tgaagtatgg agaaccgata
      901 acattccaac cgaagctcta caaccttgtg gcgacaacca ttacgaagga cttctgaaaa
      961 taaaggagat gctgagtgaa aacttcaaag tcataaatga taatatgccg gttattcttg
     1021 agcttatgtc aaaaaatgcg tacctaaagt ccatgtagtc atgaataaat atagggggaa
     1081 cttatatgct ttcccctttc tttttcacta tcaacctttt cccctaaatg taactttgac
     1141 tccgtgccgg ctccaaaatt cttgtttttt cgagaaaatc atccatttgg gggattactt
     1201 tactatccaa gcagaatata ttacgatata ggaaggtttt ggtaatgtca tttgtgttct
     1261 cttgtcttta agactgaata ttactggagg ggttatgata tgaagaaaga tattaagata
     1321 ttattactag taattactgt gctccttttt acttttctct tgcagggctg tagcaactcc
     1381 aacaattcaa ccgaccattc agcttctgag aataaccttg gtacgccttc tgaagacccc
     1441 tcgactgccg tcgatgagtc cttaccatta aatatttcct ttaaagtata cagcgaaacc
     1501 gttaaagatg atgacggttc tgatctggtt agttttaggt tttcatatcc ttatattgaa
     1561 aaccccgacg gcactgaggg aattgaggaa ataaacaact attatcaaag gcagctggaa
     1621 cattttataa gtaatgtggt gcccgaaggc aaaaaagcag ccttggatgc aaaaaaggct
     1681 gcagaggaag gcagcttcga cttttttggt ttattctttg agagggaatc aaacatttac
     1741 tacgatgaaa ataatctact gtcagttcta aatataagct atgaatatac cggaggagtg
     1801 catcctatga gctactggtc ctccgaaacc tttgatgtaa gaaccggtga gatgctgact
     1861 ctttctgata tttttggtct tagcgacgaa gaagcccttg agaaggttta ccaacttgtc
     1921 ataagtcaaa ttgaagaagc aaaagataag gaaggcatct actttgataa ttatagggaa
     1981 aacgtgaaga gttattccga aactgatttc ttgctcggtc ccaatggtat catattttac
     2041 tatcaactat acacaatagc tccttacgcc gcaggtattc caacgtttga acttccgtat
     2101 gatgagactg aactggctat cggtatcttg cccgcaaaac ctaatgacct cgaaagagaa
     2161 gtatgccttc atgcaggatg gctgatagaa gccaacaaag atatctttta caacatattc
     2221 ggcctttcca tgctcccgtt agaattgcct gaagaaattc ctgctgaaca atccttgttc
     2281 cccgtaaaag acaagaggtt tgttactttt tcggaactgg acagttacat tagaggagtt
     2341 tatctgaaga gtgaagctga ctcactgctg aatagcggca gatatgttga caaagacgga
     2401 aggctgcatg gtgacattag caaagacacg ggcatgggat attacattga ctggaataat
     2461 taccgctaca aggtaactga tataaaggaa aacagtgcaa atataacaat ttacgtagtt
     2521 gacgattcac ctgctggcag ggaagagaca actatagaag taaaaatgct gaaagaaaac
     2581 gatcgttggc tattggaaag gatgttttat taagccagaa gttttttcgc acttgaaaag
     2641 ggttgcaaaa tgaacacagt tcattttgca acccttttca agtaatttat caaaacacca
     2701 gggggagtaa acaattttat acataataat atacatagtc ttttttaaaa acatcacccg
     2761 aagaggtgaa agataaaatg aagcacaccc cgagttttag aagtgtgctt tgaaaaaatg
     2821 tatttgattg tgtatctata gtatgcttag ctcctttgcc cttttcacca cctgaactct
     2881 tctgtttaca tggagtttct catatatatt cttgatatga gtttttacag tattatttga
     2941 tatgtgcagc cttttggcaa tttcattatt tgttaaccct tcagcaatta gttttagtat
     3001 ttctagctct ctatcggtca acttttcaac gggagcttcg catgacatgt cttctcggga
     3061 tttgattgaa tataagaatt cttccaatgc cttggcataa cttgtttgac ctataagtaa
     3121 ctcaagtaat tttaaacgct cttcggtaaa aacatttgct ataagcttgt tttcaagata
     3181 caaaactcct gcaggtatgc catcaagata cagcggcaaa catgctatgg aaacatctcc
     3241 ggtgcggtca agatatgggt cttttgtaaa gataccatgc tgatagacat tgtttattat
     3301 gacagtttcc cgggtgttgg caacatatct gacaatcccc ttggatagtt tgccatcaca
     3361 ttgttttaag ggaatgggtt ttcgaaccgt ggtggagttg ctctgatggt ctatctcagc
     3421 ttcaatgaat aattcatcta ccttttctat aattaaaaaa cccttagtag cgaaaacaga
     3481 tttcatagcg gcttctaaaa tactctcggg attcgatttg aactctccgc ttatatcgcg
     3541 tattgttttc tgcaaaatgc ttagctccaa actgctcgct gtttccttat cagtaataag
     3601 ggttctcaaa atttcgtttt ttatatccct ttccccgtct ttttccaatt gttcattatc
     3661 agttaagata tcatccaata gttcaggata acgctgtcta atttctttca cttttgcaga
     3721 ggctccccat tggctataca attttaaagc atcgttcata tatatttttg ctactctttg
     3781 ttttccctcg gcaatgtaaa actttgctgc taattcacac gctattgctt cattctgctg
     3841 atagccactc tcataagcag cttgaatcgc tttttcgtat agttcctcag cctgccgttt
     3901 atttcttctt atacgcattc gctctgcctc aaccaattgg aatttgtgta aaaaattttc
     3961 cggacaggag atagcccatt ttttcatttg tttttgattg tctcttagaa ttcttgaaag
     4021 ccgtcttctt tggcttgctg gaatatcttc atatatagca gtaattatga gtgattggta
     4081 aaaattacac tctgcagaca acaaaaaacc ttgaattgat gctgtgcaat cctttacttt
     4141 ctcggcttca gctagtgctt ccctgaaatt tccagcaaag taattaatct gcatttgagt
     4201 gtaataatat gcccccagag atgctttgtc tcttgtgtct cggaaaacac ttttatcttc
     4261 atcggctttt ccattatgaa acccgatgtc actcgttaga atagaaacga ccctgctgta
     4321 aatgccaaca ttcaagttga gattatcatg ctttactttt tgcatatagt ttttacaatt
     4381 gttgatttca tcgagaatat caatcagtgg tgttcctatt atgtatctgt tttccaaaag
     4441 aacggtacgg gagtagccgt taatgagtac atctcctgct tcgacagcgt atttaaccgc
     4501 tttctgcaag tattcaattc catctttcgc atgatgagac cagtgccaga ctattgctcc
     4561 aaatgtaaaa tacacaatac attttgggta tgttttgtcg tatctctcgg ttagttcaat
     4621 tgccatctta cccaacctat cacctgcagc ataattggca aatataactc cctcaacaat
     4681 actgtaaccg acatagccaa taggagccat ttcggtattt ccgtatctta atgtatgatt
     4741 ccccgctttt ataatagcaa aagaatgtaa gtttggataa ccggtactag tggaaagtat
     4801 atactttatt aggagctcag caactttcct ctgaaccggg tccttcatct ccggcaattt
     4861 ataaagatcc tctatgcttc tgcgactcat aaaaagctta taccatagca gttctttcaa
     4921 taaatctttt gtggatggat tggctggtat taccattcca agccctttta atgcttttat
     4981 accaatttca actgctttgg cgtattctcc actaccggta tatagcaaaa tttgcaatcc
     5041 caatacatca gctttgtcaa gttcactttt tgcacgtcta ataatcagac caaatagttc
     5101 ttttgcttca gatgtatttc cgattaaaaa ctcattttga gcatattcta catacaggtc
     5161 aaagcataaa tcgtaatatg tttcccaagc atttacaggg agtaatttaa tccctgcttt
     5221 aatgcaattc accgcagtat cataagcagc agatgctttg gccttccgtc ctgcaagtag
     5281 attatactct gcgagtttca aacattcttg ctggtcagtt attaaatcta tggctctatt
     5341 catatgatcc attgcggcca gtattttgtc gttcaaatct tctcccatgc caagctgcag
     5401 aatcaagcgt ccgactttta aatgtgcagt ttttttctct gactcagtga ataatgagta
     5461 tactgcttgt ctaactctat cgtgtaaaaa ctcgtaggta gtgttttgta cctctgtgcc
     5521 tgttgataaa ggcttatggt ccccttcaat taattgcagc acaagccctt ccattattgc
     5581 cggtatgaga agagcggcta tttcagcttc cctcctattg ctgaccattg ataaagtctt
     5641 cgtgtcaaag ctattgccta tacaggacgc cagttttaaa atatctaatt gtgcttttgt
     5701 aagattatta agtctagaga gtatcaggac taaaacatca tccggtattg gaaaactatt
     5761 tatggattcc tcattccatt cccaacgccc ctttttaatg ttaaatgtta ttagtttttg
     5821 attacaaatt gatactagca tttgacttag aaaaaaggga tttccgtacg ttttgcgata
     5881 caatatttta gccagctttt ccgttttctc tcttctacta tgtaatgttt cctgcagaaa
     5941 cccagttact tcttttatat caaatggaaa gagctttatc tgagtgacag gtatctccgt
     6001 tttctctttt ttatcaatga tattttgtat gggatgattt gcatctattt cattgtcacg
     6061 ataggcggca attatgagca agtattttaa tttggtatct ttatataaaa actctaaaag
     6121 ccgtaaagaa gccggatcag cccactgcaa atcatcaaga aacatcacga gaggtgtatt
     6181 cctgtttgag aaaaccttta aaaaattttc aaaaaccatc attatacgat ttatggcttc
     6241 ctttgattga ggaatttcaa ctgctggttg acggccaatt atcatttcca aatccggcac
     6301 aatttcggta atcaatgcgc cagcattgcc cagcgaacag agtatgtctt ttttccactc
     6361 ctcaagtttc tctttgctct cagccataag ctgccttatt acaattccca aggcacttga
     6421 gatagccgca tagggcacat tttgtctcag ctggtcatat ttcccataac cgtagtagcc
     6481 ctcatcagct acaagggctt ttacagtctc attgatcagc atggtctttc ctatacctgc
     6541 agttccagtt acaaatacca actcagcact gcccgaacgg gcgcgatcaa atgcttgttt
     6601 tagcactgct gtttcccgtt ctcttccaat aagcttagta ggcaatttaa agcgagtaga
     6661 tatgtcatgc atccccagtt taaagggctc aattttcccg ctttttagcc attgtcgttt
     6721 acattctagc aagtccttca ataaaccgta tgcagtctga tatctgtccc gggcggattt
     6781 agcgaggagc ttcataacaa tatccgataa taccgaaggg atcttggcat cgatagtatc
     6841 cggcctttca ggcaccttag tggcatggag ctctgcccat tctgcagcag cgtctgcttt
     6901 gaggggaagc ctacctgtta acatnnnnnn nnnncaactc cgagagaata ataatctgac
     6961 gagaaatcgg tgccccctat ctggcctgat tgctcggggg acatgtactc tatgttatca
     7021 acaaaatgag ttttgatcgt tatcttcgaa ctgtctgtat cataaaggac agcatagcta
     7081 aaatccgtta ttctgacttc ctttatttta gggtcaataa aaatattatc gggtgttata
     7141 tttctatgga caataccgca gtaatggatg tcgcctaaac tttgtgtgag ttttattgaa
     7201 atgtcaagaa aggattctac atttaaaggt agaccttcga tatactctct cagggatatt
     7261 tccccatcgc attcgattat aagggccaga ctgttgcccg ctttttcaag gccgataggc
     7321 ttcatgatgc tctttgtgct tagctttcga gttatttcat attcgtaata cagccttgaa
     7381 acatcagcag agctggtaga ctcttctttg agtactttta tgataacagc tttaccgttt
     7441 ccacttgagt accccctgta caatattttg cttccgttct cataaacttt ttcttggatg
     7501 tcataatctt ttaaattaag cataggaagt ccccttttag cggattggga ataataaaac
     7561 ttttaaaaag gtttatgcta cactatatat ttattataca ttaactaaaa aaaattagct
     7621 atctatgtag ccctcgcggg aacacaatag atagctaata tcataatcac atcaggtaat
     7681 aagaaatagc agcttggatt gtacctataa aaaatcctat tatgcctcca agaacttcta
     7741 tttgccttaa ttcttgcctt gatagcctta taattaaatc ctcgagttcc tctatgtcca
     7801 gggaatttat tttattttct acaagttgct ttaaatccag ctggtctttt gctttttcgg
     7861 taattaccgt tttcatttca tcgaatattt cggctccgtt cctatcaatc atatctccaa
     7921 tataattgct tattgagtct ctaaaaccta aggggatata gccgggaagt ttttcactaa
     7981 ctctatttgc tatcaaattt tttatatact ttataatttc tgctttggtg tttttgtttg
     8041 tcaggctgtc cacaatctct tcaggtgaaa taatttcatg ctctaaaacc tctccgatac
     8101 ttctagagat atcaaacctc cgtttaggaa tcaaaccctg tatctgtatt cccaaaatgg
     8161 ggatttttac aggatttaag ggccgaaata ttagtttgat cgccaacatg ttagttcccc
     8221 aaccgattat cgctccgatt agaggcatca atcctagctg aaagtatggt ttcataagat
     8281 acatcaccat tttgataata gcatacataa caatcaaaaa aaaggcctct tggcctcatt
     8341 ttttcgtaag agatttggac tttgcacaga gttcgcagtg gttgcaaaat ctcacccggc
     8401 cttcaaaaat actgtataga gcatgggcaa cctcccgctt ttcattgccg ttaaaaccgt
     8461 gaatggtaat tctcctagga gcgatattta ttaaagtgct gactgttata tcgtccatat
     8521 tgacatctgg attttccttg gcaataaggt ccaagctgga atccctttct atataattta
     8581 gattttcatc cattaaaatt attttgtggt tgctaaaaac aacattaacc aggtccctct
     8641 ttggttcttg aaggtctaca aagtgacgaa gcagttttat aaactcgtta tattccttat
     8701 ccatgagata ttcatcaatg gcgatatcta ccgagtcttc cagctgcctc atatattctt
     8761 ttaaacgaaa attaacaaat ccatccaaga taattaagcc gctagttgaa agaaattcct
     8821 ggattttatg taggacatag ttttttcgct taatttgata tgagatgtcg cgttttccgc
     8881 ttatctcgtt atagtttaat atagagtagg caaaatcaaa aatcattcct tgttcagttt
     8941 tgttaaaata aaagtagttt tcccttataa ttcgcctgat gagttccggt tcccaatagt
     9001 ttataatgac atccgaaagt gcatttgcta tacaattaat aaatctttgc ttgatattaa
     9061 taaaattatt ttccataagt aacagttcat catcaacata gcaatgataa aaagagatgt
     9121 tatcttttgc tattttttta taagttattt cgaaaccatc ttcttttagt aaatcaaatt
     9181 ccagctttaa tctatcttca aaaccggcaa taggttcaat tgagcctatg gtcagcaatc
     9241 tcacaaaaac cacccctttc tcttgtaata catattatat gtatgtggct tataccatat
     9301 acaataaaac aaaggcaata ataccatatt ctgcatttgt aaagcataag tcgccaatct
     9361 ggttcatgtt atataaaaac tttcaattgc gggaataata tagttcagga ggcgatttta
     9421 tgaatggaaa gactacaagt tggatagtaa caggcgttgg tgcagttgtt acggcagtag
     9481 gatatatgac aggcgggatg attggagccg gcataacggg ttttggtctg gcccacgttg
     9541 ttctaggaat cctagatatg ttcagaccga gtgttcgtaa ctaacactat ttggttgtgt
     9601 cagttcttca tgaccttcat catttttact tgttgcataa aaatggccta gaggaagctt
     9661 cccctaggcc caaatttgta tcaatacaat ctatattttc actttcatcg gcagtttgca
     9721 aataaaaaca aaaaaatggt gggcgctgta aggattgaac ttacgacccc ttccgcgtca
     9781 aggaagtgct ctcccactga gctaagcgcc cactatctgg tgccgggaac cggaatcgaa
     9841 ccggtacgat cttttgggat cgcaggattt taagtcctgt gcgtctgcca attccgccac
     9901 cccggcatgt tttaatgcag actatattgg aggcggcacc cagattcgaa caggggataa
     9961 aggtgttgca ggcc
//
LOCUS       DAONUO010000031         9682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5402_length_9682_cov_3.055781, whole genome shotgun sequence.
ACCESSION   DAONUO010000031 DAONUO010000000
VERSION     DAONUO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5402_length_9682_cov_3.055781"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="PK768_01930"
     CDS             complement(<1..111)
                     /locus_tag="PK768_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HQA59759.1"
                     /translation="MQGKVKWFNQEKGYGFIEVEGDKDVFVHYSAIQQEGF"
     gene            265..591
                     /locus_tag="PK768_01935"
     CDS             265..591
                     /locus_tag="PK768_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59760.1"
                     /translation="MSKKGAFVKIKNFVGKLMKKRNKQVDVVELTLPGLNIKMSRNIL
                     LDTPHEITVVVPRAEIRKICQNEDCSKYQCELIYSSITLVHAPRHPLAGPAACQSEDM
                     RKSYQQ"
     gene            complement(582..2234)
                     /locus_tag="PK768_01940"
     CDS             complement(582..2234)
                     /locus_tag="PK768_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HQA59761.1"
                     /translation="MKITVKGIGAKTLDGPISLQELAKEYQDNFSSPITAAVVDNNLR
                     ELTYVLDNDATVEFLDMSSEVGMSIYLRSLVFLFVKACYDIFPDCKVLVEHSLGDGLY
                     CEIVREEPLSEDDVGTIENRMRQLVEKDIPIKKNKIPLKEAMALFQKAGMEDKVRVLR
                     HRKSDFLNVYEMDGTYDYFYGYMLPSTGYLKWFGLKFYLPGIILQHPNRSNPTQIAEY
                     HEQPKLASIFRESDRWAHILGIPDVGALNEHIISGKSGDVIRVAEALHEKKIAQIADA
                     IANERERLRIILIAGPSSSGKTTFAQRLIVQLRVNGLRPISISLDDYYLDRDKTPLGK
                     DGKPDFEALEAIDLRLFNQHLAMLIQGKEVIVPRFNFHTGKREYGDTPTRIEKDQPII
                     IEGIHGLNERLTASIPKEKKFKIYVSALTQLGIDSHNRISTTDTRLLRRMVRDSWSRS
                     TDAKQTISRWPKVLEGAEANIFPYQEEADVMFNSALVYEIAVLKKYAEPLLKKISPQD
                     PEYIKAKHLLKFLEYFEPIEDERDIPSTSIIREFIGGCSFYC"
     gene            complement(2292..3140)
                     /locus_tag="PK768_01945"
     CDS             complement(2292..3140)
                     /locus_tag="PK768_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQA59762.1"
                     /translation="MTQDLRRAPKATGNKIPLLLLVILCIIAILFPQFALAEPIPPKP
                     QANVYVFDYADLIESQDEQAMQKIAKSIDDRTKAQIVVVTVNDLSHMEIEEYALNMFR
                     NWGIGDREKNNGVLILVNKESLIANQRGRIRIEVGYGLEGAITDGKAGAILDNFALPA
                     FETGDYSRGIKDAFMAVSAEVAKEYGLDLEDGELSGLESYAVEEDDDLLTDIIIAIII
                     IFFIIITLKTRGRGRRYYRGGPFGPFGGGGFSGGGFGGFGGGGFGGGGFGGFGGGRSG
                     GGGASR"
     gene            complement(3183..3761)
                     /locus_tag="PK768_01950"
     CDS             complement(3183..3761)
                     /locus_tag="PK768_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQA59763.1"
                     /translation="MKSGLQVVIALIVIIAILGLFAFSTYNGLVTSEEAVTSAWSQVE
                     NQLQRRLDLIPNLVNTVKGYAAHEEAVFTEVTRAREKLIGAGSVADMAEANQELSGAL
                     SRLLAIAENYPQLKADANFRQLADELAGTENRIAVARMDYNNAVQTFNTKIRRFPTVI
                     FANMFGFERKEYFQAEEGASQAPVVDFSTENK"
     gene            complement(3893..4687)
                     /locus_tag="PK768_01955"
     CDS             complement(3893..4687)
                     /locus_tag="PK768_01955"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA59764.1"
                     /translation="MSPTVAINMEKLTKIFYTNQKKVVALDNIDLSVKEGEFLCIVGP
                     SGCGKTTLLRILAGLEKQTWGKLVIRSKNPEKPLTSMVFQGDSVFPWMTVKENVAYSL
                     RIKGLPKSERLRIAGRYIKLMGLEGFADAFPHQLSGGMKQRINVARAFVSDPEILLMD
                     EPFGALDEQNRLLLQQELLKIWKGSGKTSLFITHSIDEALVLADRIMVMTAHPGKVKA
                     IVPVNIPRPRDILSLRSSPNFGALFKQIWELLQEEVLKAKSMELKT"
     gene            complement(4693..5541)
                     /locus_tag="PK768_01960"
     CDS             complement(4693..5541)
                     /locus_tag="PK768_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA59765.1"
                     /translation="MSLMEETLQEQLSRIIIKEAKGESKKAVFDKGLLLKVLAFLSPS
                     LLLVLWEIFARLELIDIRLFSSPVMIFKTMMPMLYSGELLYHTFISAQRIVLGFLAGA
                     VPGVILGLSMGLFAPVRASLMPMVSATFPIPKLAIMPLILIIFGLGEASKIFTIGIGV
                     FYLVLINTMAGVLNIDDIYLDVAKNFGAKPFQFYTTVAFPGALPMIFAGFKLGMGTAL
                     LLIVAAELSAAKAGVGWMIWRAYDMFDIEKMFVALITLAFLGYIFSNLLEIAEKIVIP
                     WQNNRR"
     gene            complement(5602..6687)
                     /locus_tag="PK768_01965"
     CDS             complement(5602..6687)
                     /locus_tag="PK768_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA59766.1"
                     /translation="MFLVLVMLLSAFVIGCGRQAVENKVEEVSQKQAIEQTDEEEVEV
                     QPLSPKVKVTVGMKEVVSDSGVLIGMAKGYYEELGIEIESVAFNTGQDMINALGAGQL
                     DVGCTVTASGLFNAMLRGIPIKIVADKGINVAGQGYYRLMIRKDLVDEIKDFEDLRGR
                     KLAVVGTASLDEIALDRVLNQAGMTTADVDLQVIRSFPDIVAAMSNKSIDGGMIIEPY
                     VTAAMDADIADPWKDPSEYDPDAQTALLIYGKSMLERPEVANSFMIAYLKAIRDYNNA
                     FFKDINKDEIISILAEYSVVTDKELYEKMFPVGLNPDGFVRMKGVQMDLDWYKERGLL
                     KGDLNAEDVVDNSFAEYAVKVLGKYEK"
     gene            complement(6896..7171)
                     /locus_tag="PK768_01970"
     CDS             complement(6896..7171)
                     /locus_tag="PK768_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HQA59767.1"
                     /translation="MNTVSLNLMFKALSDPIRRKILLMLKKRDMTAGEIAEEFDISKP
                     SISHHLNILKQAGMVIDERKGQYIYYSINTSVFEDVVSFALQLSARK"
     gene            complement(7200..7862)
                     /locus_tag="PK768_01975"
     CDS             complement(7200..7862)
                     /locus_tag="PK768_01975"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HQA59768.1"
                     /translation="MADKTKSRLPINWFVVALILSLYVLGYFLYPHLPEQVPSHWNMA
                     GEVDGYSSRTFHILFFPSLILFLYLVMSFAPVLDPRPESYRKFQGVYEGFRYLMIGLL
                     MLLYMATTLYALGYTFSIGKIVRFAIGLLLVFTGNYFGKIRHNYTFGIKTPWTLASEE
                     VWNKTHRMSGPLWVVVGFIWMISVLIAEKLAFAISMGSFITVSIYGCVYSYILFQRLK
                     NQ"
     gene            complement(8079..8345)
                     /locus_tag="PK768_01980"
     CDS             complement(8079..8345)
                     /locus_tag="PK768_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59769.1"
                     /translation="MFLFNKESVYIGYSLEELAKVRYVLNREGIKYSYRVVNRSGQWS
                     GPGSRRGRFGSFGMNMKYEKQYTVYVHKKDVEKAKYLVNSVLHQ"
     gene            complement(8556..>9682)
                     /locus_tag="PK768_01985"
     CDS             complement(8556..>9682)
                     /locus_tag="PK768_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQA59770.1"
                     /translation="FAVWYLVFDNFTAGLINAVSVLVIACPCALGLATPTSVMVGTGK
                     GAEHGVLIKGGEHLERAHKIKALVLDKTGTITKGQPEVTDIISQGDMTPEEILMFAAI
                     AEKNSEHPLGEAIVDRARQKSMDIVDAEDFMAIPGHGIYAKIQGKDVYLGNRRLMKSK
                     NIDTNDVEAILEKLEDEGKTAMIMAMDNKIQGIIAVADTLKDHSKKAISELKEMGIEV
                     WMITGDNERTAKAIAKQVGIDNVMAEVLPEHKAREVEKLKSQGNITAMVGDGINDAPA
                     LAAADVGIAIGTGTDVAMEASDITLMSGDLRGIVTAIKLSRATMRNIRQNLFWAFIYN
                     TIGIPFAAFGFLSPAIAGAAMAFSSVSVVTNALRLKRFKP"
BASE COUNT         2538 a         2351 c         1930 g         2863 t
ORIGIN      
        1 aaagccttcc tgctgaattg ccgaatagtg aacaaagaca tctttgtctc cttcgacttc
       61 gatgaaacca tagccttttt cttggttaaa ccacttaact ttaccttgca attattcatt
      121 cctcctaaaa acttaaatag aatgggccct tctttattca actatatcac accgaaaaag
      181 caaagtcaaa tcaaagattt ttgttccatt tcatataatg aaagtaacta aggcggcaag
      241 aatcatgaga caaggagaat ttttatgtca aaaaaagggg cttttgtaaa aataaaaaac
      301 tttgttggta aattaatgaa aaaaagaaat aagcaagtgg atgtggtgga gcttaccctg
      361 cccggcctta atataaagat gtccaggaat attcttttag atacacccca tgaaataacg
      421 gttgtggtcc ccagggctga aattcgcaaa atttgtcaaa atgaagattg cagcaaatac
      481 cagtgtgagc tcatatacag cagtataacc ctggttcacg ccccaaggca tccgctagcg
      541 ggcccagctg cttgtcaatc ggaggatatg cgaaaaagct atcagcagta aaagctacag
      601 cctcctataa actcgcggat aatggaagtg ctgggaatgt ccctttcgtc ttcaatgggt
      661 tcaaagtact cgagaaattt cagcagatgc ttggccttta tatattccgg atcctgcggg
      721 ctgatttttt tcagcagggg ttcggcatat tttttgagca cagctatttc ataaacaagg
      781 gctgaattga acataacatc cgcttcttcc tggtaaggga aaatattcgc ttcggcacct
      841 tctaggacct tgggccatct ggatatggtc tgttttgcat ctgtggacct gctccaactg
      901 tccctaacca tgcgccgtag caatctcgtg tcggttgtgg agattcggtt gtggctatct
      961 atccccagct gggtcaaagc acttacgtat attttaaatt ttttctcctt gggtatggaa
     1021 gccgttaaac gctcattcaa gccgtgaata ccttctatga taatgggttg gtctttttct
     1081 attcttgtag gagtatcgcc atattcccgc ttgcccgtgt gaaaattgaa cctgggcact
     1141 attacttcct tgccctgaat cagcattgcc aaatgttggt taaacagtcg aaggtcaatg
     1201 gcctccaggg cttcaaagtc gggttttcca tcttttccta gcggggtctt gtccctgtcc
     1261 aaataataat cgtccaagga aatggatatg ggccttagcc cgttgaccct aagctgaact
     1321 atgagccgct gggcaaaggt ggtcttaccc gatgaggatg ggccggcgat taggattatc
     1381 ctcagacgtt ccctttcatt ggcaatggcg tcggcaatct gtgctatctt tttttcgtga
     1441 agggcttcgg ccacccttat gacatccccg ctcttgcccg atattatgtg ctcatttaag
     1501 gctccgacat cgggtatgcc caaaatgtgg gcccatcggt ccgattctct aaaaatactg
     1561 gccagctttg gctgttcatg gtactctgct atttgtgtgg gatttgatct attgggatgc
     1621 tgaaggatga ttcccggcag ataaaacttc aggccaaacc atttcaagta gccggttgat
     1681 ggcagcatat atccgtaaaa gtaatcgtaa gtgccatcca tctcgtatac gtttaaaaag
     1741 tcgctctttc tgtgtcttaa aaccctgacc ttgtcttcca ttccggcttt ttggaacaaa
     1801 gccatagcct ctttgagggg tatcttgttc tttttaatgg gtatgtcctt ttccaccagc
     1861 tgccgcatgc gattctctat agttcctaca tcatcctcgc tcaatggctc ttcgcgaacg
     1921 atttcgcagt aaaggccgtc acccagtgag tgctctacaa ggaccttgca atcggggaag
     1981 atatcgtagc atgccttgac gaacagaaat acaagggagc ggagataaat gctcatgccc
     2041 acttccgagg acatgtccaa gaactctacc gtagcatcat tatcaaggac atatgtcaat
     2101 tctctcagat tgttatccac tacggcagca gtaataggtg aggaaaaatt gtcttgatat
     2161 tcctttgcca gctcctgaag gctaataggg ccatccaagg tctttgcacc aatacctttt
     2221 acggttattt tcaatacgat cacctcttta cttactttgt atatatgata ccctaaattt
     2281 gccctggcaa actatcgact cgctcctccg ccaccactgc ggccgccgcc aaatcctcca
     2341 aagccaccgc ctccaaatcc tcctccgccg aacccgccga agcctcctcc ggagaagcct
     2401 ccaccgccaa agggaccgaa aggacctccc ctataatacc tgcgtcccct tccccttgtc
     2461 ttgagtgtaa ttattatgaa aaagattatt atgatggcga tgattatatc tgtcagtaag
     2521 tcatcatctt cttcaacggc gtagctctca aggccggaaa gttcgccatc ttccaggtca
     2581 aggccgtatt ctttggcaac ttctgcagag acggccatga aggcatcttt aattccccgg
     2641 gaataatcac ccgtttcaaa ggccggcaat gcaaaattgt ccaggatggc tccggccttg
     2701 ccgtcagtta tcgcaccttc caagccgtac ccgacttcaa tccttatgcg acctctttga
     2761 tttgcaatga ggctctcctt atttaccagg attaagacac cgttgttctt ttccctgtcg
     2821 cctatgcccc aatttctaaa catattaagg gcatattcct ctatctccat atgggataga
     2881 tcgttgaccg tcaccacgac tatctgggcc tttgtcctgt catcaatgga tttggctatt
     2941 ttttgcattg cctgttcatc ctggctttct atcaggtcgg cataatcgaa tacatataca
     3001 ttcgcctggg gttttggagg aatgggctcg gctaaggcga attgcgggaa aaggattgcg
     3061 ataatacaga gtattacaag caaaagcagc gggattttat tgcccgttgc cttaggtgct
     3121 cttcttaagt cttgagtcat ttaagttcac cgctttcaaa ttcatttgac aaacaaaagg
     3181 cactacttgt tttcagtact aaaatctact accggagcct ggcttgcccc ttcctccgcc
     3241 tggaagtact cttttctttc aaagccaaac atgttggcaa atataacggt gggaaatctc
     3301 cttatctttg tgttaaaggt ttgcaccgca ttattgtaat ccatgcgggc aactgctatc
     3361 ctgttttcgg tgccggccag ttcatccgca agctgcctga aattggcatc ggctttaagc
     3421 tggggatagt tttctgcaat ggccagcagc cttgataaag caccggacag ctcctggttg
     3481 gcctctgcca tatctgcgac agaacctgcc ccgatgagtt tttccctggc ccgtgtaact
     3541 tctgtaaaca ctgcttcctc atgggcggcg taacctttta ccgtgttgac caaatttggt
     3601 attaaatcca aacgccgctg caattgattt tcaacctggc tccatgcgct ggtcaccgct
     3661 tcttcagatg tgaccaagcc gttgtaagtt gaaaaagcaa acaaacctag aattgcaatt
     3721 atcactatta gagctattac cacttgtagt ccacttttca atgaagaccc tcctgacttt
     3781 tgtttaatct cttttatatt aattattata cacgaaatat ttaactcgtg agaaaaaatt
     3841 tctaaatcgc aataaaataa agcagcgcct gctgctcaaa tactcaacaa agtcatgtct
     3901 tcaactccat agacttggcc ttcaacactt cttcctgaag cagctcccaa atttgtttaa
     3961 agagagctcc gaaattcggc gatgaacgta aagacaggat atctctaggt ctcgggatat
     4021 ttacgggaac aatggcttta accttgcccg gatgggcggt catcaccatt atcctgtcgg
     4081 ccaacacgag ggcttcgtca atgctgtgag ttatgaagag ggaagtcttg ccgctgccct
     4141 tccatatctt caagagctcc tgctggagga gcaagcggtt ttgttcgtca agggcaccga
     4201 agggctcatc cattaacagt atttccgggt cactgacaaa agcccgggcc acattgattc
     4261 tctgcttcat ccccccggac agctggtgcg gaaaagcatc ggcaaatccc tcaaggccca
     4321 tcaattttat atagcggccg gctatcctga gcctttcact tttgggaagg cccttgattc
     4381 tcaagctata ggcaacattt tccttcaccg tcatccacgg aaatacagag tcaccttgaa
     4441 agaccatgga tgtcaatggt ttttccgggt tttttgacct gattactagc ttcccccaag
     4501 tctgtttttc caaacctgcc aaaattctaa ggagggttgt cttaccgcat ccgctgggtc
     4561 ccacaatgca caaaaattcc ccttccttta ccgataaatc gatattatcg agggctacca
     4621 cctttttttg attagtataa aagattttcg tcagtttctc catgtttata gctacggtgg
     4681 gactcaaatt tttcacctcc tgttgttttg ccagggtatg actatttttt cggctatttc
     4741 cagtaaatta gagaaaatat atcccagaaa ggccaaggtt ataagggcta cgaacatctt
     4801 ctcgatgtcg aacatgtcat aggcccgcca gatcatccag cccacgccgg ctttagctgc
     4861 cgaaagttct gccgctacaa tgagcagcaa agctgtcccc attcccaact taaaacctgc
     4921 aaatatcatg ggcagcgctc cggggaaggc taccgttgtg tagaactgaa agggctttgc
     4981 cccgaagttt ttggccacgt ccagatatat gtcatctatg tttagtacgc cggccatcgt
     5041 atttatgagc accaggtaaa aaacaccgat gcctatagtg aatatcttgg aggcttcccc
     5101 aaggccaaat attatcagaa tcaagggcat gatggccagc ttcggaatag ggaaggtggc
     5161 tgataccatg ggcattaggc tggctctgac cggtgcaaaa agtcccatgg aaaggcctaa
     5221 aatgacaccc ggcacagccc ctgccagaaa tcccagcact attctttggg cactgataaa
     5281 ggtatgatac agtagctcac cggaatagag catgggcatc atggttttga aaatcatgac
     5341 ggggcttgaa aaaagcctta tgtctataag ctcaagtcgg gcaaaaatct cccataaaac
     5401 aaggagcagt gatggtgaaa gaaaagctaa tacttttaaa agcaagcctt tgtcaaatac
     5461 tgcttttttg gattcgcctt tggcctcttt tattattatc cgagacaatt gttcctgaag
     5521 ggtctcttcc attaaactca tgttaattcc cctttctatt aaggtggccg aaaaccgtat
     5581 tcgattttcg gccacagctg cctatttttc atatttccct agcactttta ccgcatattc
     5641 ggcaaagctg ttgtcaacca cgtcttccgc atttaaatct ccttttaata gtcctctttc
     5701 tttataccag tccaggtcca tttgaacccc tttcattcgc acaaagccgt cgggatttaa
     5761 acctaccggg aacatctttt cgtaaagttc cttgtccgtg actaccgaat actcggcaag
     5821 gatagagatg atctcatctt tattgatatc tttaaagaaa gcgttattgt aatcccttat
     5881 tgctttgaga taagcaatca taaagctatt ggccacttcg ggcctttcca gcatgctctt
     5941 accataaatc agcagggccg tttgcgcatc tggatcatac tccgaggggt ccttccaggg
     6001 atccgcgatg tctgcatcca tcgcagcggt gacatagggc tcgattatca ttcctccgtc
     6061 aatgctcttg ttgctcatgg ccgccactat atcgggaaag ctcctgatta cctgcaggtc
     6121 aacatcagcc gtggtcattc ctgcctggtt taaaaccctg tctaaggcaa tctcatccag
     6181 ggaagcggtg cctactaccg caagcttgcg ccccctcaaa tcctcaaagt ctttgatttc
     6241 gtccaccagg tcctttcgaa tcatcaggcg gtaatagccc tggccagcaa cattgatgcc
     6301 cttatctgct acaatcttta tgggtatgcc ccgaagcatg gcattaaaaa gaccagatgc
     6361 cgttacggtg cagcctacat ccagctggcc tgcacccagg gcgtttatca tatcctggcc
     6421 ggtattgaaa gccaccgatt cgatttcaat gcccagttcc tcatagtaac ccttggccat
     6481 gcctatgagc acacctgaat ccgatactac ctctttcatc cccacggtca ctttcacttt
     6541 tggagaaagc ggctgtactt ctacttcctc ttcgtcagtt tgctctatcg cctgcttttg
     6601 tgatacttct tccaccttgt tttccacagc ctgccgacca catcctatga cgaaggcgga
     6661 aagcagcatc accagtacta aaaacatagc tataggcctc aatagaacca cccctttaaa
     6721 aaaattgcct tctttatatt attcaaaaag cctcaaaggg gttacggcaa aataaaaaag
     6781 gctcaacctt cggataatcc cgctagtttg agccttaaat gaatattccg ggacgggtac
     6841 aaaatattca tttcgaatga atgtgaatat tttgtacccg tcccggaata ttcattcatt
     6901 ttcgggcgct aagctgcaga gcgaaactca ccacatcttc aaagacggat gtatttatcg
     6961 agtagtaaat gtactggcct tttctttcgt ctattaccat gcctgcctgt tttagtatgt
     7021 taagatgatg ggaaatgctg ggctttgata tgtcaaactc ttccgcaatc tctcctgcag
     7081 tcatatcccg cttttttagc atcaaaagga ttttccttcg tatggggtct gacagggctt
     7141 taaacattaa gtttaggctc acagtgttca tcatctcctt cggttgccgt gtagccatct
     7201 cattggtttt taagtctttg aaataagata taggaataga cacagccgta aatgctgact
     7261 gttataaagc ttcccatgga aattgcaaaa gctaactttt cagcaatgag aacactgatc
     7321 atccatatga agccaacaac aacccagagt ggcccggaca tcctgtgggt cttgttccac
     7381 acttcttcac tggcaagggt ccagggtgtt tttatgccaa aggtatagtt atgccttatc
     7441 ttgccaaagt agttgcctgt aaaaacaagc agtagaccta tcgcaaatct aactattttg
     7501 cctatagaaa aggtatatcc caatgcatat agtgtcgtag ccatataaag taacatgaga
     7561 agtccaatca ttagatagcg gaatccttca tatacacctt ggaactttct ataactttcc
     7621 ggcctgggat ctaaaactgg tgcaaatgac attactagat ataagaaaag aatcaggctc
     7681 gggaaaaata aaatgtgaaa agtccttgaa ctataaccgt ctacttcacc ggccatattc
     7741 cagtgtgacg gcacctgttc gggtagatgt ggatagagaa aatatcccaa aacatacagc
     7801 gatagaatca gtgctaccac aaaccaattt atcggcaatc ttgatttcgt tttatctgcc
     7861 atacgagcac ctcccattat ttagataatt agacaattat ctaaatatat tttaacataa
     7921 aagttgctta ataacaacac gctatcagga aaaatattat tacaaaataa ttacaggcat
     7981 tataataggg gctagccttt gctacccgtt tcggcatggc attatattgc acgatatgag
     8041 aatatcccgt cttattctat gacgactcaa catttgtttt attgatgtaa aacactattg
     8101 acaagatatt ttgccttttc aacatccttt ttgtgaacat aaacagtgta ttgtttctca
     8161 tatttcatat tcattccgaa actgccaaac ctgcctcttc ttgaaccagg tcccgaccac
     8221 tggcccgaac ggtttaccac tctataagaa tactttatac cctccctatt aagtacatat
     8281 ctaacctttg ccagttcttc taaggaataa cctatgtata cgctttcttt gttaaaaaga
     8341 aacatataag gcagcctccc atacggagta ttaatgtgta aatgatgcat tatttttact
     8401 ccctgatttc tagcaattca attataccag aatttacatt attgtatatc aaattaaata
     8461 ataatatata aaaaagatat aggaaagatg attatattaa tccatctaac ctatatctat
     8521 tagtgtggca atcttcgctt tttactttat ttagattaag gtttaaatct tttaagtctt
     8581 agcgcattag ttaccactga aactgaactg aaggccatag ctgctccggc gatggccggg
     8641 cttaaaaagc caaaggctgc aaaggggatt ccaatagtgt tgtagataaa tgcccaaaac
     8701 aggttttgcc taatatttcg catagttgca cggctcagct ttattgcggt aactattccc
     8761 cttaaatcgc cgctcattag agtgatatca gatgcttcca tggccacgtc ggtgccggtg
     8821 ccgattgcga tacctacatc cgctgcagca agagcgggcg catcgtttat gccatctccc
     8881 accatggcgg taatattgcc ttggcttttc agtttttcta cctctcttgc cttatgctcg
     8941 ggcagcactt ctgccataac gttatctata cccacttgct ttgctatggc tttggcggtt
     9001 ctctcgttat ctccggttat catccatacc tcaatgccca tttcctttaa ttcgctgata
     9061 gctttcttgg aatggtcttt taaggtatcg gctacggcta ttatgccctg gattttatta
     9121 tccatggcca tgatcatggc cgtcttgcct tcatcttcca atttctctaa gatggcttcc
     9181 acatcatttg tatctatgtt tttggatttc atcaatcgcc tgttgccaag gtatacatct
     9241 ttgccctgga ttttggcata aataccatgg cccggtattg ccatgaaatc ttctgcatcg
     9301 acaatatcca tgcttttttg tctggctcta tccactatag cctcacctaa gggatgttcc
     9361 gagtttttct ccgcaattgc cgcaaacatc aatatctctt caggagtcat gtctccctgt
     9421 gagattatgt cagttacttc cggctggcct ttagtaatgg tgcctgtttt gtccaggacc
     9481 aatgctttta ttttgtgggc tctttccagg tgttctccgc ccttgatcaa aaccccgtgt
     9541 tctgcacctt tgccggtgcc taccattacc gaggtaggtg tagcaaggcc caatgcacag
     9601 gggcatgcta tgacgagaac cgatactgca ttaatcaagc ctgccgtaaa gttgtcaaag
     9661 acaaggtacc acacggcaaa gg
//
LOCUS       DAONUO010000032         9539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5505_length_9539_cov_3.509701, whole genome shotgun sequence.
ACCESSION   DAONUO010000032 DAONUO010000000
VERSION     DAONUO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5505_length_9539_cov_3.509701"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="PK768_01990"
     CDS             complement(<1..154)
                     /locus_tag="PK768_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HQA59771.1"
                     /translation="MRRAHEIDFQIFGDDMQFVEISLDPEETVVAEAGAMMYMDSAIR
                     SDTVFGD"
     gene            complement(422..916)
                     /locus_tag="PK768_01995"
     CDS             complement(422..916)
                     /locus_tag="PK768_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59772.1"
                     /translation="MKKLLVLLLVLALIFSVAGCGVKKKIENKIGEAIGEKIIEGATG
                     QKVDIEGDKVTIGGKEGSLTFGGGQWPDNDLVKSIPEFRDGKIEAVMSSEETVTIAIE
                     EVEQRAFENYLANIKKNFTKDTFEMTTNEVISYSGANEKIKVHLSYDIPGKSMFIAIG
                     KQAE"
     gene            1302..2558
                     /locus_tag="PK768_02000"
     CDS             1302..2558
                     /locus_tag="PK768_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQA59773.1"
                     /translation="MKNTMDKMIDEFNATVGAEKGIIINVTSISGSASLHEKLIMAAY
                     GDPGAPELPDISTAYPKTALILAEKGLLIDLGEQFSEEELAAYLPRFLEEGRIKDGKL
                     YVFPIAKSTEVFFVNKTIFNKFASETGIGFKDLDTIEGIIRAAEKYYQWTDSQTPDIP
                     HDGKTFFMVDSLFNFTLVGCKQLACDFINDNQIDCFVPAFSRVWDCFYEPVLRGYVAI
                     FDGYSTDLIKTGDVVCYTGSTAGVAFLPARVTYPDNTTEPTDFAILPYPIFEGGKKIA
                     VQRGAGFCVLKSTKEKEYAAGIFLKWFTKPEQNLKFVLSTGYLPVTLEAFDRIMAQEI
                     EAPTDDNIRKLLDVAVEMQQNYDFYIPPVFEGIDALEKQYEQNLKRIAKSSRSEFLKL
                     IQYENPDTAYSKVSEGVFEQFIHMKL"
     gene            2574..4172
                     /locus_tag="PK768_02005"
     CDS             2574..4172
                     /locus_tag="PK768_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQA59774.1"
                     /translation="MIQKSGLKQRLRIISKMGRSMQLRLFLFLLALATTMILGIVAIL
                     FVTGTLTAGIKESSKFMERELNYIHDNIYKQFGSLSVLAVELSSDLSKSIEASMAERG
                     YEVTDIKNHPELLEGLVSSEYQRMLFSLAKSKSSGVFLILNTTFNDKLENADNSRAGL
                     YIKNMEPNIISSSSPTIVLLRGFANIGRKNSIPLHVQWSMEFDVSDAPYYHIPMQEAS
                     KSKLPLSRLYYWSPAVTLPGCSEEVMLCSVPLIDSNGNVFGVCGFEISAMLFKLSQMP
                     DSSKFARTFCLLAPIKDDIIDSSAALYSGGYPAQNLTLKEEDLHIEKKTNSLFSYAQE
                     SGDIFVGLHRPVRLYPEDSAFAKHKWISAIMIPAEDIKYSLTRFNLQLAFLLMLLMIL
                     GAVISFFLSRQYLKPIVDGIDMIKAKGLKGPPRTNIPEIDDLIEFLSMHNEKIQEQKD
                     DTLASEVLDKFLENTKTLSPAERAVFDLYVEGYNAKEISEKLCLSINTIKTHNKRIYM
                     KLNVASRKELLLYVQMLKEAGREP"
     gene            complement(4179..5243)
                     /locus_tag="PK768_02010"
     CDS             complement(4179..5243)
                     /locus_tag="PK768_02010"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HQA59775.1"
                     /translation="MASLWWIIESTDNEKFPYLLSIKKGEDTLLRLRVQNRWPGAGTH
                     IFCLRDPGDGTKTIEEVERVPIINLSRYGKRISVVLDRPINKRCDFLFLKKKYKKKEG
                     EYEQIFWRTQQGLKERKPRVKLAAQGDFGIHILIDVNEKYPWKFKDCTVERKALDAGD
                     YALLRDDGIIAVVERKTFDNLRTEIANLPILHQKLCELEAYAHTALVVEANYSDFLNP
                     DKLMFYTPSFMAKALAELSALHPKINIVFAGNRKLANEWTLRFFEAIVSNANDGLPGM
                     VAEQIENYEPSSNFRGGIYYDIRMEILDSFPAEFSISDVREKFPDTDDGTIRRVLRDL
                     KDKNIIHSTGRGRNSKWVKI"
     gene            complement(5365..5799)
                     /locus_tag="PK768_02015"
     CDS             complement(5365..5799)
                     /locus_tag="PK768_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQA59776.1"
                     /translation="MERIKLVVENQIGETKGTPLEGAVRQNFTGETKEAGIYLAMARL
                     AQRQGYPEIAEVLKSIAWDEAEHAARFAELNGMIQEDLFENIKMMLEGEIASNKDKKQ
                     AADKAADVGLETARDYFNETAKDEARHARMLEGILARYKNCC"
     gene            complement(5843..6289)
                     /locus_tag="PK768_02020"
     CDS             complement(5843..6289)
                     /locus_tag="PK768_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59777.1"
                     /translation="MNVDIEALINAVSKCCLGEAQCGACDMDNCLIGYCKKMLTTSLK
                     DNTEFIDGGVENLPYYDTRFYDEVDVADTIGFLLNQCRNCNLYHDEACIINIIRSALE
                     IILLGQAQEYKGSVLIYLNNIRDADEAVADKILEAFNRWKEAASNN"
     gene            6644..7642
                     /locus_tag="PK768_02025"
     CDS             6644..7642
                     /locus_tag="PK768_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HQA59778.1"
                     /translation="MSKKVLALALAAVLILVMLAGCGGGKSTPAPENGGEEEEAKPQE
                     QIFLNIATGGTAGTYYPLGGALSEIWNKNIPGVNSTAQSTGASVANINLLKEGKADII
                     MVQNDITYYAANGVELFEEKYEDIRGLAILYPETVQLVTLANKGINSVADLKGKRVAV
                     GAAGSGAEANARQILNAAGITYDDITEQYLSFAEASNNLKDGNVDAAFLTAGFPTAAV
                     TDIAAQHNLQLISLDDDLIEKLMNEYPYYTKITIPAGTYSGVDEDIQTVAVQAMLAVK
                     SDMDEDLAYNLVKQMYDNLDRIGAAHSVGELIKPETGTEGMPIELHPGAQKYFDEL"
     gene            7688..8203
                     /locus_tag="PK768_02030"
     CDS             7688..8203
                     /locus_tag="PK768_02030"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HQA59779.1"
                     /translation="MKKSLKFFAILVTAALLFFFVNYRLVNTKVLIIEAEGKNIKKII
                     AVPQKKFTISFIHSVQKTPVYEVFEICEDNRLILTETTYSSLGVGLPFTEEEGKFTNE
                     QGKFKLTGMNREFTSIPILVSPIPKHTIIVGENTYPLLSIVAPDDLVKITAADRWILG
                     RRNNFTMEGTE"
     gene            8207..>9539
                     /locus_tag="PK768_02035"
     CDS             8207..>9539
                     /locus_tag="PK768_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HQA59780.1"
                     /translation="MTEIKDNEFEQAEVVSEEFLRKVDKESDYRVLTGFFAKLIAAIA
                     ITFSVFQVYTAAFGTLDAMLQRAVHLAFALCLIFLLYPARVSWPRDKIHPIDVVFAIL
                     GAAAPLYIVVFYKQLVMRAGLVTTTDYIVGIIAIILILEAVRRVVGIPMVCVAILFLV
                     YALYGRQLPGVLAHRGADLPTLVQHLFYTTEGIFGIPLGVSSTFIFLFILFGAFLEKT
                     GLGQLFIDLSNAVAGWAAGGPAKVAVIASALEGTVSGSSVANTAGSGSFTIPMMKKLG
                     YEPEFAGAVEATASTGGQLMPPIMGAAAFLMAEFIGMPYFRIVTAAIIPSLLYYFGVW
                     TQVHFEAKRLGLKGMSRDELPKIGDVLREKGHLLVPLLALIYLLAKGYTPMRAALVAI
                     VLTVGCAMLRKNTRISLLDIIEGLENGARGALGVVAATACAGIIVGVVTLTG"
BASE COUNT         2659 a         2023 c         2029 g         2828 t
ORIGIN      
        1 cgtcaccgaa taccgtatct gacctaatgg cactgtccat gtacatcatg gcccccgcct
       61 cggcgactac ggtttcttcc ggatcaaggg agatctccac aaactgcata tcgtcaccga
      121 aaatctgaaa atcaatttca tgagcccttc gcatcaataa ttcctccttt attcttaaag
      181 taattctata agtcttgact cgctatgcgt ttccttcctg ttgccacaat gagccctgct
      241 gcaattgcca ggatcgctgc acacccgatg ccaatttata acgttgagtt agaggattta
      301 tcgcgccctt cctttagtaa ttgagctatt tgcaattgaa ggaccgcaag gttatgcagt
      361 ccttcaattt cacacctgta attcacagct cgttctgctt tatactttta cttaatcctc
      421 attattctgc ttgcttgcca atggcgatga acatgctttt gccgggtatg tcgtaggaaa
      481 gatgaacctt aattttttca tttgcgccgg aataagatat cacttcattt gtggtcattt
      541 caaaagtatc cttagtaaag ttctttttga tattcgcaag gtagttttca aaagcccttt
      601 gttctacttc ttcaatagca attgtgacag tctcctcact gctcataaca gcttcaatct
      661 tgccgtccct gaactcaggg atgcttttga ccaaatcgtt atccggccac tgcccgccac
      721 cgaacgttaa ggaaccttct tttcctccga tggttacctt gtctccttct atatcaacct
      781 tttggcctgt tgcgccttct atgatctttt caccgattgc ctcaccgatc ttgttttcga
      841 tctttttttt cacgccgcat ccagctaccg aaaaaatgag cgccaacaca agcagcagca
      901 ccaatagctt tttcatggca cacctccttt gttactttat tatgatcgac caagcctttg
      961 gctttccgtc tacttcaatt tattaattat ttacgttatt gtaatcaccc aaggggtgat
     1021 tagggtaaga aaagaggggt gatttttggg gtgatttatg atataatatc tacgctataa
     1081 tttattagaa tattttaaat cgtaaaactt gtcaaaaatt gtggattcca atagaaagct
     1141 gtcgcaaggg ggggattgcg atagtaaaag cgaagcggtt cttaagtgtt gttttgctgt
     1201 ctgtgagtat ggctcttttt tgtggctgtc agggtgacga tgatttattg gaccccagaa
     1261 atcccgttac tttaaccctc tggcataact atggagggca aatgaaaaat acgatggata
     1321 aaatgataga tgagttcaat gcaaccgtcg gagctgaaaa aggcatcata atcaacgtta
     1381 cttccatatc cggttctgca agcttgcatg aaaagctgat aatggcagca tacggggacc
     1441 ccggagcccc ggagcttccc gatattagta ccgcttaccc gaaaaccgct cttatcttgg
     1501 ctgaaaaggg gctcctcata gatttagggg aacagttctc ggaggaagaa ctggcagcgt
     1561 acctgcctcg ctttttggag gaaggtcgga ttaaagatgg aaagctttac gttttcccaa
     1621 tagcaaaatc tacagaagtg ttttttgtaa ataaaacgat atttaataaa tttgccagtg
     1681 aaaccggcat cggtttcaaa gatttggata caattgaggg gattatcagg gccgcagaga
     1741 agtattatca gtggactgac agtcaaactc ctgatattcc ccatgatggc aagacgtttt
     1801 ttatggtaga ttcccttttc aatttcaccc tcgttggctg taaacaattg gcttgtgact
     1861 ttattaatga caatcaaatt gactgctttg tacccgcatt ttctagggtg tgggattgct
     1921 tttatgaacc ggtactccgg ggatatgttg ctatttttga cggctacagc acggacctga
     1981 tcaaaacagg tgatgttgtg tgctatacgg gttctacggc gggagtggct ttcttgcctg
     2041 ccagggttac gtatccggac aacacgacag aacctaccga ttttgccatt ttgccttatc
     2101 caatctttga agggggtaaa aaaatcgccg tacagcgggg ggcaggtttt tgtgttttaa
     2161 aatcgacgaa agagaaagaa tatgccgccg gaatttttct caaatggttc acaaagccgg
     2221 aacaaaactt gaaattcgtt ctttcaaccg gatatcttcc agttaccctg gaggcttttg
     2281 acaggataat ggcccaagag atagaggccc cgactgatga taatattagg aagcttcttg
     2341 atgtggctgt tgagatgcag cagaattacg acttttacat accgccggta tttgaaggta
     2401 tcgacgctct tgaaaagcag tacgagcaaa acctaaaaag aattgctaaa agttcaaggt
     2461 cggagttttt aaagctgatt caatatgaaa atcctgatac ggcatacagc aaagtcagtg
     2521 aaggtgtttt tgagcagttt atccatatga agctttaata caggatggat agtatgatac
     2581 aaaaatctgg tttaaaacaa agacttagaa taataagtaa aatgggcagg tctatgcagc
     2641 ttagactttt cttgtttctg cttgctcttg caacaaccat gattctcgga attgtcgcga
     2701 ttctattcgt aaccgggaca ctaacggccg gaattaagga aagcagcaaa ttcatggaac
     2761 gtgaattaaa ttatattcat gacaatattt ataaacaatt cggcagcctt tccgtcctgg
     2821 ccgtggagct gtcgagtgat ttgtccaaaa gtatagaagc aagcatggct gaaaggggat
     2881 acgaagtgac cgatatcaaa aatcatcccg aactcttaga agggctggtc agcagcgaat
     2941 atcagcgcat gctgttttcc ctggccaaat ccaaaagcag cggtgtgttt ctcattttaa
     3001 acacgacgtt caatgacaag ttagaaaatg ccgataattc tagagcggga ctatatataa
     3061 aaaatatgga accgaatatt atcagttcca gctctcctac tatagtcctt ttgagaggtt
     3121 ttgcaaatat aggtaggaaa aactccatac cccttcacgt ccagtggagt atggagtttg
     3181 atgtcagtga cgcaccgtat tatcatattc ccatgcaaga ggcaagcaaa agcaaattgc
     3241 ctttatcaag gctttattat tggagtcctg ctgttacatt acccggttgt agcgaagaag
     3301 tcatgctgtg ctccgttcct ttgattgatt ccaatggaaa tgtcttcgga gtgtgtggct
     3361 ttgaaataag tgcaatgctt tttaagctca gccaaatgcc cgatagcagc aaatttgccc
     3421 gcaccttttg tttgctcgct ccgatcaaag acgacatcat tgatagctcc gcggccttgt
     3481 attccggagg ctatcctgca cagaacttaa ctttgaagga agaagatctg cacattgaaa
     3541 aaaagacgaa ctctctcttt tcatatgctc aagagagcgg cgacatcttt gtgggacttc
     3601 accgacctgt tcgtctatat cctgaagact ccgcctttgc caagcataag tggatatcag
     3661 ctataatgat acctgccgag gacatcaagt actccttaac ccgttttaat ttacaattag
     3721 cttttttgct catgctgcta atgattttag gggcagtaat atcatttttc ctaagcagac
     3781 agtatttaaa accgatcgtt gacggcatag atatgatcaa ggcaaagggg cttaaaggac
     3841 caccaaggac taatattcca gagatagatg accttattga gtttctttct atgcacaatg
     3901 aaaaaatcca ggagcaaaaa gatgatacgc ttgcatcgga agtcctagac aagtttttgg
     3961 aaaacacaaa aaccctttca ccggcagaaa gggcggtctt cgacctttat gttgaggggt
     4021 acaatgccaa ggaaatttcc gagaaactct gtctcagcat caatacaatc aagacccaca
     4081 acaagcgcat ctatatgaaa ctaaacgtag cttcaaggaa ggaattacta ttatacgtac
     4141 aaatgcttaa agaagcaggg agggagccct gacagttctt aaatcttcac ccatttacta
     4201 tttctgcccc taccggtaga atgaatgatg ttcttgtctt taagatctct caaaaccctt
     4261 cttattgttc cgtcatcggt atcgggaaat ttttccctga catcggatat gctaaactca
     4321 gccggaaagc tatccaatat ttccattctt atatcatagt atattccgcc tcggaagtta
     4381 gaagatggct cgtaattctc tatctgttca gccaccattc cgggcaggcc atcgtttgca
     4441 ttagatacta tagcttcaaa aaacctcaaa gtccattcat ttgccagctt tctgttccca
     4501 gcaaatacga tgtttatttt aggatgcaga gcggaaagtt cagccaaggc cttggccata
     4561 aatgaaggtg tataaaacat taatttatct ggatttaaaa aatcagaata attggcttcc
     4621 acaacaagtg ctgtgtgggc atatgcctca agttcgcaca acttttggtg taaaatagga
     4681 agatttgcga tttcagttct taagttatca aaggttttcc tttccacgac agctatgatt
     4741 ccatcatccc ttaatagggc gtagtcacct gcatccagag cttttctctc tacggtacag
     4801 tccttgaatt tccagggata tttttcattg acatctatca aaatatgaat cccgaaatcc
     4861 ccttgagctg caagtttcac tctgggtttt ctttctttta aaccctgttg ggttctccaa
     4921 aagatttgtt cgtattcgcc ttcttttttc ttatattttt tcttcaaaaa taaaaagtcg
     4981 caccttttgt taataggccg atctaggact acggagatgc gctttccgta gcgacttagg
     5041 ttgattattg gcacccgctc gacctcttcg atagtctttg ttccatcgcc aggatctctc
     5101 aaacaaaaaa tatgagttcc agcacctggc catctgtttt ggaccctaag ccgcaaaagg
     5161 gtgtcttcac ctttttttat gctgagcaag taagggaact tttcattatc tgtgctttca
     5221 ataatccacc atagagaagc catcattagt ctcctttaaa ggttttgtgt agttaaatta
     5281 tagcgcatcg acctggcagg gggacgtttc gtttgccagg ttgaaaaacc tggcaaacga
     5341 aacgtccccc tgccaggtcc gcgtttagca gcagttcttg tagcgggcca agattccttc
     5401 cagcattctg gcgtgtcttg cttcgtcttt ggcggtttcg ttgaagtaat ccctggcggt
     5461 ttcgaggcct acgtcggcgg ctttgtcggc ggcctgtttt ttatccttgt ttgaagcgat
     5521 ttcgccctcc agcatcatct ttatgttttc aaataggtct tcctggatca tgccgtttag
     5581 ctccgcaaat ctggcggcgt gctcggcttc gtcccaggcg atggatttta ggacttccgc
     5641 aatttccgga tagccctggc gctgggcaag tctggccatg gccaggtaaa tacccgcttc
     5701 ttttgtttca ccagtaaaat tctgccttac agctccctca agtggcgtgc ccttggtttc
     5761 tccaatctgg ttttctacca caagctttat tctttccata ttatacctcc ctcttttaat
     5821 gttctcatgt ttttatcggc aattaattat tgctggctgc ttctttccaa cgattaaagg
     5881 cttccaagat tttatcggca acagcttcat ccgcatccct gatattattc aggtagatta
     5941 agacgctgcc cttgtattcc tgggcctggc caaggagtat aatttccagg gcgcttctta
     6001 taatattaat aatgcaggct tcgtcgtggt aaaggttgca gtttctgcac tgatttaata
     6061 aaaaacctat agtatctgca acgtccactt catcataaaa tcttgtatcg taataaggta
     6121 ggttttcaac accgccatct ataaattccg tattatcttt aagagaagtg gtgagcatct
     6181 ttttacaata tccgatcaag caattgtcca tgtcgcatgc accgcactgg gcttctccca
     6241 agcagcactt tgaaactgca tttatcaagg cttcaatatc tacattcata ccctttctcc
     6301 ttcccaaaag gctaaaataa taatcgcaca tcataatgat aattgttctt aatataaata
     6361 taaataaact ggaatctaaa gtcaagcttt ctgttgtaat acagttaatt atgcatgatt
     6421 tttaggcaaa atgagtaaaa taaaggggac aaatacagaa tattccttat aactcaattt
     6481 tgacaaatta atagtaaagt attaaaatat taattagtat gtgaactgta aatgacaatg
     6541 tttgcactaa tgtatacttg acaaattgca tacaagtatg ttattatttt ttcgttatgt
     6601 tcacctacaa aaaataataa attaatttaa agggggatta atcatgagta aaaaagtgtt
     6661 agccctagca cttgctgctg tgctaatcct ggtgatgctg gccggctgtg gcggaggaaa
     6721 aagcactcct gcaccggaaa atggtggtga ggaagaagag gcaaaaccac aggagcaaat
     6781 attcttgaat attgccacgg gcggaacggc cgggacctac tatccattag gcggagcact
     6841 ctctgaaatt tggaataaga acatacccgg agtcaattct acagctcaaa gcacgggtgc
     6901 atcagttgca aatatcaacc tgttgaagga aggcaaagcc gatatcatca tggttcaaaa
     6961 tgacataact tattatgcgg caaatggcgt ggagcttttt gaagagaaat atgaggacat
     7021 aaggggcctt gccattttgt atcccgaaac agttcagttg gtcactctag caaacaaggg
     7081 aatcaattcc gttgctgacc tcaaaggcaa gagagttgcg gtaggcgcgg caggaagtgg
     7141 cgctgaggct aatgcaaggc agattcttaa tgctgcagga attacttatg acgacattac
     7201 tgaacagtac ctatcctttg cagaagcttc caacaacctc aaagacggca atgtcgatgc
     7261 agctttttta actgcaggat tccccacagc agccgttacg gatatagcgg ctcagcataa
     7321 cctacaattg atttccttag atgatgattt aatcgaaaaa ctgatgaatg aatatccata
     7381 ttatacaaaa attactatac ctgctggcac ctacagcggt gtagatgaag atatccaaac
     7441 cgttgctgtt caggcgatgc tggcagtaaa aagtgacatg gacgaggatc tggcttacaa
     7501 cctcgtaaaa cagatgtatg acaatcttga tagaattgga gcagctcact ctgtaggtga
     7561 acttataaaa cctgaaacag gcaccgaagg tatgcctata gaacttcacc caggcgccca
     7621 gaagtatttt gatgagctat aataaaggag gtaactgagc attggagcaa tccaatgctc
     7681 agcttttatg aaaaaatctt taaaattttt tgcaatactg gtcacagcgg cattgctatt
     7741 tttttttgtt aattatagac tagtaaatac gaaggtttta attatagaag ctgaagggaa
     7801 gaatatcaaa aaaataatcg cggttccaca gaaaaaattc acaataagtt ttatacactc
     7861 ggtgcagaaa acacctgtat acgaggtctt tgaaatatgc gaggacaaca ggttaatcct
     7921 aacggaaacc acttactctt ctttgggagt ggggctccct tttacggaag aagaggggaa
     7981 gtttacaaat gaacagggca agttcaagct gacaggcatg aatagagaat ttacttccat
     8041 tcccatactg gtttcgccta ttccaaagca taccattatc gttggagaaa atacttatcc
     8101 cttactgtct attgtagccc cggatgacct tgtgaaaatt acggctgctg acaggtggat
     8161 acttggaagg cgtaacaatt tcacgatgga agggactgag tgagaaatga cggaaatcaa
     8221 agataacgaa tttgaacaag cagaagtggt aagtgaagag tttcttcgaa aagttgacaa
     8281 ggagtcagat taccgagttt taactggctt ttttgctaaa ctaatagccg ctattgcaat
     8341 aaccttttct gtatttcaag tttacaccgc agcttttgga accctagacg ccatgttgca
     8401 aagggccgta catcttgcct ttgctttatg cctgatattt ctcctgtatc cggcaagagt
     8461 tagttggccg agagacaaga tacatcccat cgatgtcgtc tttgccatct taggggctgc
     8521 agcgccactg tacatcgttg tattttacaa gcagctggta atgagagcgg gtcttgtcac
     8581 tactaccgac tacatcgtcg gaatcatagc tataatttta atacttgaag cagttagaag
     8641 agtggttggt atacccatgg tgtgtgtggc aatattgttt ttggtatatg ccctttacgg
     8701 aaggcagctt ccgggcgtcc ttgctcacag gggagccgat cttccaacac tggtccagca
     8761 tctcttttac accaccgaag gtatttttgg tataccccta ggcgtgtcat caacctttat
     8821 attccttttt attctgttcg gtgctttctt ggaaaaaacc ggcttgggcc aacttttcat
     8881 agatctttcc aatgccgttg caggctgggc ggcaggaggg ccagccaagg tagccgttat
     8941 agcatctgcc ctggaaggaa ccgtatcggg aagctccgtg gcaaatacgg caggttcggg
     9001 tagctttacc attcccatga tgaaaaagct gggatatgag cctgaatttg ccggtgcggt
     9061 agaagctaca gcttccaccg gtggtcagct tatgccccct ataatgggtg cagcggcctt
     9121 tctcatggca gaattcatag ggatgcctta ctttagaata gtaacagctg ccataatccc
     9181 ctcacttttg tattattttg gagtgtggac tcaggttcac tttgaggcta agcgcttagg
     9241 tcttaaggga atgtcccgtg atgagctgcc aaagattggc gatgtgctca gggaaaaggg
     9301 ccacctttta gttcccttac ttgcattgat atatctttta gcaaagggtt atactcccat
     9361 gagagcagcc cttgtcgcca tagtgctaac agtaggatgt gctatgctca ggaaaaatac
     9421 ccgtattagc ctccttgata taattgaagg ccttgaaaat ggtgcaagag gtgcattagg
     9481 tgtcgttgcc gcaacggctt gtgccggtat tattgtaggt gttgtgacac ttacaggtc
//
LOCUS       DAONUO010000033         9392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5604_length_9392_cov_4.235729, whole genome shotgun sequence.
ACCESSION   DAONUO010000033 DAONUO010000000
VERSION     DAONUO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5604_length_9392_cov_4.235729"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2051)
                     /gene="nifJ"
                     /locus_tag="PK768_02040"
     CDS             complement(<1..2051)
                     /gene="nifJ"
                     /locus_tag="PK768_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQA59781.1"
                     /translation="MAKVMKSMDGNTAAAHVAYAFTDVAAIYPITPSSPMAELVDEWS
                     AHGRKNIFGQQVKVVEMQSEAGAAGTVHGSLAAGALTTTFTASQGLLLMVPNMYKIAG
                     ELLPGVFHVSARAVAGHALSIFGDHSDVMACRQTGFALLASSSVQEVMDLAGVAHLSA
                     IKGRVPFLHFFDGFRTSHEIQKIEVLEYEDLAKLVDYDAIREFRKRALNPERPVTRGT
                     AQNPDIFFQAKEAANPFYLAIPEVVEEYMNKINELTGRNYKLFNYYGHPEAERVIVAM
                     GSVCETIEETVDYLMAKGEKVGVLKVRLFRPFSTKHFLAEMPKTVKKIAVLDRTKEPG
                     SLGEPLYQDVCTVYIEADKRPTIVGGRYGLGSKDTTPTHIKAVFDNLKADSPKNHFTI
                     SIVDDVTNTSLPVEEQIDTTPEGTTCCKFWGFGSDGTVGANKSAIKIIGDNTDMYAQA
                     YFSYDSKKSGGVTISHLRFGNKPIRSTYLIDKADFIACHNPAYIGKYDVLAGLKDGGT
                     FLLNCQWTPEELEEKLPASVKNYLAKHNISFYIINAVDIAQEIGLGGRINMIMQAAFF
                     KLANIIPVEDAVKHLKDAIVKSYGHKGEKIVEMNYQAVDKGIEALVKVDVPEAWADAK
                     DEAAAEVDAPDFIKNVADVMNRQEGDKLPVSAFAGREDGTFPMGTARYEKRGIAVNVP
                     E"
     gene            complement(2414..3259)
                     /locus_tag="PK768_02045"
     CDS             complement(2414..3259)
                     /locus_tag="PK768_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQA59782.1"
                     /translation="MKDIVVVTDSSCDLPEEVIDNYPIKVLPMPVSVKENPEMDISGL
                     SIKEFYDAMRRGEILPTTSQVVVSAFMRCFEECINNGQTPLVLGLSSKLTSSFDSALL
                     AKENMKIEDAVIIDTKCASLGLGLVVLKAAKMAKEGSSIQDIAEEIRHYALHMEHIFT
                     VDSLDHLKRGGRISAAQAFVGGLLNIKPILHFVDGAIHPLEKVRGRKNVVKRMVEVMG
                     QRVKNPEKQVVGISHADNEELAMELAEAVKNEFKVKDIMISWIGQVIGAHTGPGAVAL
                     FFQNA"
     gene            complement(3373..4554)
                     /locus_tag="PK768_02050"
     CDS             complement(3373..4554)
                     /locus_tag="PK768_02050"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HQA59783.1"
                     /translation="MIQKVILIVLDSVGVGELPDAAKYGDEGSNTLGNVAKAVGGLSL
                     PNMQKMGIGNIIDIEGVPAVKEPTAAYGKAAEKSAGKDTTTGHWELAGVILDRPFPVY
                     PDGFPPELIEEFEKRIGRKVLGNKVASGTEIIKEPGDEHVKTGRPIVYTSADSVFQIA
                     AHEEVIPVKQLYDICKIAREILRGEHGVARVIARPFVGKSGEYLRTYNRADFSLKPPH
                     DTLLDKVVRAGMEVIGVGKIWDIFAGQGITKAMHTEGNMDGVDKTLEAIDMIQKGLVF
                     SNLVDYDMLYGHRNDPVGYAKALEEFDGRLPEILEKLGDNDVLIITADHGCDPTTVST
                     DHSREYVPVLVYGDRIKAGVKLGTLSSFSDIGQTVTDFLGCLSLKNGRSFKQLVFKDA
                     K"
     gene            complement(4905..5156)
                     /locus_tag="PK768_02055"
     CDS             complement(4905..5156)
                     /locus_tag="PK768_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59784.1"
                     /translation="MKIGAGRQKKDDVIDPAVGIWIYKKVGDKVTKGEAFAKILANDE
                     EKLHWAAEESKAAFEFSREPIARRKVIWAKITKDGVHEL"
     gene            complement(5122..6231)
                     /locus_tag="PK768_02060"
     CDS             complement(5122..6231)
                     /locus_tag="PK768_02060"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HQA59785.1"
                     /translation="MFFVPEIIEKKKRGLKLSKEEIEFIVNGYVDGSIPDYQVSALLM
                     AIYFKGMDEEETTNLTMAMAHSGEMVDLSAIKGIKVDKHSTGGIADTTTLVLLPLASS
                     VGVKVAKMSGRGLGHTGGTVDKLESIPGFRCELDGKDFIDAANSIGAAITGQSKNLVP
                     ADKMLYALRDVTATVDSIPLIASSIMSKKIAGGADKIILDVKFGSGAFMKNYDDALKL
                     GSLMVKIGGLAGRETVAYITDMDQPLGLAIGNSIEVIEAAETLKGRGNEDLLSLCLEF
                     ASEMMVLAEIESDKEKAIERLKASIDAGEALKKFKEIIANQGGNPEVLEDYSLLPKAK
                     YELEVVAPTDGFEANRCYPPRNYKYENRGRQAEKG"
     gene            complement(6605..6997)
                     /gene="cdd"
                     /locus_tag="PK768_02065"
     CDS             complement(6605..6997)
                     /gene="cdd"
                     /locus_tag="PK768_02065"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQA59786.1"
                     /translation="MERIDEKKLIKKTLSAMANAYAPYSKFPVGACIVTADGEEITGC
                     NIENASYGLSMCAERVALFKAYSEGKKDIVALAVAANVDEPVSPCGACRQVISELAPK
                     AIVYLTNKDGSKVKKMTVEELLPYGFSL"
     gene            complement(7051..7812)
                     /locus_tag="PK768_02070"
     CDS             complement(7051..7812)
                     /locus_tag="PK768_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQA59787.1"
                     /translation="MKLGVCQMAVSENLGVNTKKIISFIKEAALQDINIVGFPEMALT
                     GYTKEILADENLNNMVQDSLSEIKKACKFHNTAVLVGHPYKAGEKLYNSATVFLPDGS
                     TFTYDKKYPTELESKYFEAGKKEPLVFPFGDAKIGVIICRDQNYPELCRALTKAGAQF
                     IYILSAHFYDPKEARWKLEKNRAIPITRAVENKVYVLLSNFVGSHLGKVSLGNSLIAD
                     PDGAVVVSAGETEEGLLSVSIDDFSRRKNEFHSEN"
     gene            7981..9087
                     /locus_tag="PK768_02075"
     CDS             7981..9087
                     /locus_tag="PK768_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQA59788.1"
                     /translation="MKVVVLGGAGDMGSRAVRDLATKKEVTELIIADININAAENLAA
                     KLGEKVKAIYVDANEPKTLVKAIEGSDVVASAMGPFYKYEKVAVKAAIAARVNYVSIC
                     DDYDAVESILPLDEEARKLGLSILTGLGWTPGISNILARKGAEQLDEVEEINIYWAGS
                     ANDATGLAVTLHTIHIFTGMVTSFMDGKRVEIPAGSGKEKVEFIEPVGFVDMYHLGHP
                     EPVTLPLYIPSVKTVTLKGGLKESYLNQLAIAVARLGLTNTAGKKQALGKLLKALLPV
                     LEKIQRPAVPMSGIRVDIKGYLQGKKQHLVYQAADHMDNLTGVPLAIGALMMGKGEIT
                     RKGVFAPEAAVDPDRFIDELAQRNIKVVCTKGIS"
     gene            9104..>9392
                     /gene="yfcE"
                     /locus_tag="PK768_02080"
     CDS             9104..>9392
                     /gene="yfcE"
                     /locus_tag="PK768_02080"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HQA59789.1"
                     /translation="MKIAIISDTHGSITAFNRAMEVAGPCDFIVHAGDILYHGPRNPL
                     PEGYKPQELANAINALEVPFVAAAGNCDAPIDQVLLSVPIQAPYAMLYIENF"
BASE COUNT         2385 a         2318 c         1965 g         2724 t
ORIGIN      
        1 cattcgggaa cattcaccgc aatgccccgc ttttcatacc tggcggtgcc cattgggaat
       61 gtgccatctt cacgacctgc aaatgcactg acaggcagtt tgtcgccttc ctggcgattc
      121 attacgtcgg caacattctt gatgaagtca ggtgcatcta cttcagcagc agcttcatct
      181 tttgcatcgg cccaagcttc aggcacgtct actttcacca aagcctcgat gcccttgtcc
      241 acagcctggt agttcatttc gacgatcttt tcacctttgt ggccgtagga tttaacgatg
      301 gcatctttta gatgttttac tgcatcttct acggggatga tattagcaag tttgaagaat
      361 gcagcctgca ttatcatatt tattcttccg ccaaggccga tttcctgggc aatatctact
      421 gcgttgatta tatagaagct gatgttgtgt ttagcaagat aatttttaac cgatgcggga
      481 agcttttctt ccagttcttc gggagtccac tgacaattta gcaggaatgt gccgccatcc
      541 tttaggcctg ccaagacatc gtatttgccg atgtatgcag gattgtggca agcgataaag
      601 tctgctttgt cgatcaagta ggtagagcga attggtttat tgccaaagcg caggtgggaa
      661 atcgttacac caccggattt cttggagtca taggagaagt atgcctgggc atacatatcg
      721 gtgttgtcgc cgataatctt aatagcgctc ttgttggctc caacagttcc gtctgaacca
      781 aagccccaga atttacagca tgtggttcct tcgggagttg tgtcaatttg ctcttctacc
      841 ggaagtgaag tgtttgtcac atcgtccacg atgcttatcg taaagtggtt cttgggcgaa
      901 tcggccttta agttgtcaaa gaccgcctta atgtgggtag gtgttgtatc cttagagcca
      961 aggccgtagc gaccgccaac aattgtagga cgtttgtctg cttcgatata cacggtgcaa
     1021 acatcctggt acaatggctc gcctaaagag ccgggttctt ttgtcctatc aaggacagct
     1081 attttcttga ccgtcttggg catttcagct aggaaatgct ttgtagagaa gggtctgaac
     1141 aggcgaactt tcagcacgcc tactttttca cccttagcca tcaagtaatc gacggtttcc
     1201 tcgatggttt cgcaaacgga acccattgcc acaattaccc gctccgcttc gggatggccg
     1261 tagtagttga acaacttgta attgcggccg gtaagttcgt tgattttgtt catatattct
     1321 tcaacaactt caggaattgc aagatagaaa ggatttgcag cttcctttgc ctggaagaat
     1381 atgtccggat tttgggcagt gccgcgtgtt acgggacgct cgggatttag agctctcttc
     1441 ctaaattctc tgatcgcatc gtagtccact aattttgcca aatcctcata ttcgagaact
     1501 tctatcttct ggatttcgtg ggatgttcta aaaccatcaa agaaatgtaa gaagggtacc
     1561 cttcctttta tggctgatag gtgtgccaca ccggccaaat ccattacttc ctgaacgctg
     1621 ctggaagcaa gtagtgcaaa accggtctgg cggcaggcca ttacgtctga atggtctcca
     1681 aaaatagaca gggcatgtcc tgcaacagct cttgcgctga catgaaatac accgggcaat
     1741 agctctccgg cgattttgta catattcgga accatgagca gcaatccttg ggatgctgta
     1801 aaagtcgtgg tcagggctcc tgctgccaat gaaccgtgta cagttccagc agctcctgct
     1861 tcagattgca tttccacaac ttttacctgc tggccaaaaa tattttttct tccgtgagca
     1921 ctccactcat cgacaagttc tgccatgggg gacgatggag ttattgggta aatagctgct
     1981 acatcggtaa atgcatatgc tacatgggca gcagcagtgt tcccgtccat ggacttcata
     2041 acttttgcca ttggttctcc tctcctttgt ttattattta agacaatatg caaaggctct
     2101 cttgtttagc tagcttgtga taaaaatcac ttaatgaaaa ttaccttaaa tacgttttaa
     2161 acacactgtc gggagccttg atacaaaaca caccaaaaaa cattataaca taacaaaaat
     2221 actttttcca cttaaggtct taaatcacct ctctcaaata aagatgtgta agacacgcat
     2281 gttttttgta tactgtttgt taaattctac atttttctgc aaaatcctcc attgatatgc
     2341 gagggcaaca ataaaaaaat ctcctttaaa gcaagacatt tagacaatgt ctgctcccag
     2401 gagataagga cgatcaagcg ttttggaaaa aaagagcaac tgctcctggc ccggtgtgtg
     2461 cacctatcac ctgcccgatc cacgatatca taatgtcctt tactttaaac tcatttttca
     2521 ctgcttcagc aagctccatg gccagttctt cattgtcagc atggcttatg ccaaccacct
     2581 gcttttcggg attttttacc ctctgcccca ttacttccac cattcgctta accacatttt
     2641 ttctgcccct gaccttttcc aagggatgaa tggctccatc aacaaagtgc aaaatgggtt
     2701 ttatgttgag caagcctcca acaaaggctt gtgcagcaga tattcttcct ccacgcttta
     2761 gatggtctaa agaatccacc gtaaaaatgt gttccatatg taaagcatag tgtcttattt
     2821 cctccgctat atcctgaatg ctacttcctt ccttggccat ttttgccgct tttaacacga
     2881 ccaagcccag tcccaggctt gcacattttg tatcgattat caccgcatcc tcaatcttca
     2941 tattctcttt agccagcagt gccgaatcaa agctgctggt aagttttgaa gaaaggccaa
     3001 ggactaaggg agtctgcccg ttattaatgc actcctcgaa gcaccgcata aaggcggaaa
     3061 ccactacttg ggatgtcgta ggcagtatct cacctcttcg cattgcatca taaaactctt
     3121 taattgataa accggaaatg tccatctcgg ggttttcttt tacagagacc ggcattggca
     3181 aaacttttat tggataatta tctataactt cctccgggag atcgcaagag ctatccgtca
     3241 ctacaactat atctttcatg gcgcttcacc tctcacctta taatggcaaa aatattatac
     3301 cacaattaca gtattgataa aataagcttt gttcatttag cacgtactag aatacttttc
     3361 ccacttttcc cgctactttg catccttaaa aaccagctgc ttaaaactcc tgccgttttt
     3421 aaggctcagg cagcccaaaa agtccgtcac cgtctggcct atatcagaaa aactggagag
     3481 tgtcccaagt ttgacccccg cctttatcct gtcaccgtag actagaaccg gcacgtattc
     3541 cctggagtgg tctgtgctta ccgtagtagg gtcgcaacca tggtctgcgg tgataattaa
     3601 cacatcgtta tctccaagtt tttccagtat ctcgggcagg cgcccatcga actcctccag
     3661 tgctttggcg taacctaccg ggtcatttct gtggccgtaa agcatatcgt aatcaaccag
     3721 gtttgaaaaa accaagcctt tttgtatcat atctatcgct tctagggttt tatctacacc
     3781 atccatgttg ccttccgtgt gcatggcttt agttatgccc tgaccggcaa agatatccca
     3841 tatcttcccg actcctatga cttccatgcc ggcccttacc accttgtcaa gcaaagtgtc
     3901 atggggcggt tttaaagaaa aatctgccct gttataagtc cttaggtatt cgccggattt
     3961 gcccacaaag gggcgcgcta taacacgggc caccccatgc tctccccgga gtatttcccg
     4021 ggcaatcttg cagatatcat aaagctgttt tacgggaata acctcttcat gggcggcgat
     4081 ctgaaatact gaatctgcag aggtatacac gatgggtctt ccggttttta catgctcgtc
     4141 tcccggctct tttattatct cggtgccgga tgcgacctta ttgcccagga cttttcggcc
     4201 tatccttttc tcaaattcct ctattagctc cggcggaaaa ccatcgggat acacgggaaa
     4261 gggcctatcc aatataacac cggcaagttc ccagtgtcca gtcgtcgtat cctttcccgc
     4321 agacttttcc gcagcctttc cgtacgcagc tgtcggctct tttacagctg gtacaccttc
     4381 tatatcaatg atatttccga tacccatctt ctgcatgttc ggcagcgaaa gtccgcctac
     4441 tgccttggcc acattgccca gcgtgttgct gccctcatcg ccgtatttgg ccgcatccgg
     4501 caattctccc actcccacac tgtcgaggac aattagaatt accttttgaa tcatgttatc
     4561 agtccttttt tcgtatttgg ggtttttgac aagaaaagaa ctctttactc aaagccttaa
     4621 tcttgagctg atgttaacaa gctaattctc tagagcaatg aaagaaattt tcttcatgtt
     4681 ttagaaagat aatgacaaac gaacccttta agcaatgccg tcgtcgctct taagtttggt
     4741 ggaagctgag caggggcagc gtgcaggagg cacgctggca ggcgctcggc aggatgccga
     4801 attgcctgcg aacccctgcg gagtgcagca ctgaacttaa gagcaagagg cattgattat
     4861 aaggtgagct gtcgttatct tctctaaatt tcattaaaaa gattttatag ttcatgaact
     4921 ccgtcctttg ttatctttgc ccatattacc tttcttcttg cgatgggctc ccttgaaaat
     4981 tcaaaggcgg ccttactttc ttcggctgcc caatgaagtt tttcttcatc atttgccagg
     5041 attttagcga atgcttcgcc ctttgttact ttatcgccaa cttttttgta tatccatatt
     5101 cctacggcag ggtctatgac atcatccttt ttctgcctgc cggccccgat tttcatactt
     5161 gtaattccga ggcggatagc atcgatttgc ttcaaaaccg tctgttggtg ctacaacttc
     5221 cagctcatat tttgcctttg gcaaaaggga atagtcctct aaaacttcgg gattgcctcc
     5281 ctggttcgca attatctcct tgaatttctt tagggcctcc cctgcatcaa tacttgcctt
     5341 aagccgctct attgcctttt ctttgtcact ttcaatctcg gcaagcacca tcatctccga
     5401 agcaaactct aaacacaggg aaagcaggtc ttcattgccc ctgcctttta aagtctccgc
     5461 cgcttctatc acttctatac tgttaccgat ggcaaggcct aggggctggt ccatgtcggt
     5521 aatgtatgcc acggtctccc ggccagccaa tccaccgatt ttaaccatca agcttcccaa
     5581 tttcagcgca tcgtcataat ttttcataaa agcgccgctg ccaaatttca cgtcaaggat
     5641 aatcttgtcg gcaccgccgg caatcttttt gctcatgatg gaactggcga ttagcggaat
     5701 tgaatcaacg gtggccgtca catctcttaa ggcgtataac atcttgtctg ccggaaccag
     5761 attcttcgat tggcccgtta tagcagcacc gatgctattt gctgcatcga taaagtcttt
     5821 gccgtccagc tcgcatctaa agccgggaat ggattccagc ttgtcaacgg ttcccccagt
     5881 atggccaagg cctctgccag acatcttggc cacctttacc cccaccgaag atgccagggg
     5941 caaaagcacc aatgtggtcg tgtccgcaat gcctccggtg gaatgtttat caacttttat
     6001 tccttttatg gctgataaat ccaccatctc tccagagtgg gccatggcca ttgtgaggtt
     6061 ggtcgtctct tcctcatcca ttcctttaaa gtaaatggcc attagcaatg cagatacctg
     6121 gtaatcggga atgcttccgt ccacatagcc attcactata aactctatct cctctttgga
     6181 aagctttaaa ccccgcttct ttttctcaat tatctccggc acaaaaaaca taccaacacc
     6241 tgccttttta tttaaaatag acagttcttt tccttaccac cttgctggag atggcaagac
     6301 ccttccttat agttattccc gtaaacacga cgtcaccttg caaaaaagac cgtttagttt
     6361 gagtcgaaga aaaactctta agcttggctc tgcactccgc acggattcgt aggcaattcg
     6421 gcatcctgcc gagtgacaag agcctcctcc tgcacgctgc ccctgctcgt ttattccaag
     6481 cttaagaata ataatgtcct tgctttattg ggttcgcttg tcgttatctt acctcaattt
     6541 tcgctgtgaa atgaattatg tgtctattgt tgaccagtaa atcaatggaa tttgtttata
     6601 aaatctaaag cgaaaatccg tagggcagca gctcttccac tgtcatcttt ttaactttag
     6661 agccgtcttt attggttaga tacacaatgg ccttaggtgc cagttccgat attacctgcc
     6721 ggcatgcccc gcaaggtgac accggttcat ctacatttgc agccaccgcc aaagctacga
     6781 tgtccttttt cccttccgag tacgccttaa aaagggcgac gcgctcggca cacattgaaa
     6841 ggccgtatga ggcattctca atattgcagc ctgtaatttc ctcaccatcg gcggttacga
     6901 tacaagctcc caccgggaac tttgaatacg gcgcgtaggc atttgccatt gccgacaagg
     6961 tcttcttgat gagctttttc tcatcaatcc tttccataca accactacct ttgataaata
     7021 tgttttaatt cattagaaat gattctatcc ttaattttcg ctgtggaatt catttttcct
     7081 tctggaaaaa tcgtcaatag agactgaaag aaggccttcc tcagtttcac ctgcagaaac
     7141 taccacggcc ccgtccgggt cggcaattaa gctgttcccc aggctaacct tgccaagatg
     7201 cgagcccaca aaatttgaaa ggagcacgta tactttattt tccacagccc tggttatggg
     7261 aattgccctg ttcttttcca gcttccacct ggcttctttt ggatcataaa agtgggctga
     7321 aagtatgtat ataaattggg ctcctgcttt tgtcaaagcc ctgcaaagtt ccgggtaatt
     7381 ttggtctcta catattataa cgcctatttt tgcatcaccg aacggaaaca ctagtggctc
     7441 ctttttgccc gcttcaaaat atttcgactc cagttccgtg ggatattttt tgtcataagt
     7501 aaaggtgctg ccgtcaggta aaaaaacagt ggcactgttg taaagttttt cgccagcctt
     7561 gtagggatgg cccaccaaca cggctgtatt atggaacttg caggcttttt taatctctga
     7621 cagggaatcc tgcaccatat tgttcaggtt ttcatctgct aaaatttcct tggtataacc
     7681 cgtaagggcc atttcgggaa atcctactat attaatatct tgcagggcag cctctttaat
     7741 aaaactgatg attttcttgg tattaacacc gagattttct gaaactgcca tttggcaaac
     7801 tcccagcttc ataaattaca cctcttttgt caaattacac taaaactatt atatcctaag
     7861 atttgctatt aaaaaaagtc aaaacatcat tttttaaaag tcccaaagta tatcgcatgc
     7921 ctatttattt ttgttgtata ataaggacat atatggcatt ttgtgaaagg agataaactt
     7981 atgaaagtag tagttttagg cggtgccggt gacatgggca gcagggcagt aagggacttg
     8041 gcaacaaaaa aggaagtgac tgaactcatc attgccgata ttaatataaa tgccgctgaa
     8101 aacttggcag caaagctggg cgaaaaggtc aaagcaattt acgtagatgc aaacgagcca
     8161 aaaaccttgg tgaaggctat tgaaggcagt gatgtagtcg caagtgccat gggtcctttt
     8221 tataaatacg aaaaggtggc ggttaaagcc gctatcgcgg ctagggtaaa ctacgtaagt
     8281 atatgtgatg actatgatgc agttgaaagc attttgcccc tggatgaaga ggcaagaaaa
     8341 cttggtttga gcatcttgac aggcttgggc tggactcccg gcattagcaa tattcttgcc
     8401 cgaaaagggg ccgagcagct tgatgaagtg gaagaaatta atatctactg ggcaggcagt
     8461 gccaacgatg ctaccggcct tgccgtaacc ctccacacca ttcacatatt taccggcatg
     8521 gttacaagct tcatggacgg taagagagtg gaaattcccg ccggcagcgg caaggaaaag
     8581 gttgagttta tagagcctgt aggtttcgtg gacatgtacc atcttggcca ccccgagccg
     8641 gtcaccctgc ccctatacat accaagtgtt aaaacggtta cacttaaagg cggcctcaag
     8701 gaaagctacc taaaccaatt ggccatagcc gtcgccagat tgggtttaac caataccgct
     8761 ggcaaaaaac aggccttggg aaagctgttg aaagctctac ttcccgtcct tgaaaagatt
     8821 caaagacctg ctgtccccat gtcgggaatt cgagtagaca taaagggata tttgcagggc
     8881 aagaagcagc acctggtgta ccaggcagcc gatcacatgg ataacctgac gggtgtgccc
     8941 ctggccatcg gcgctttaat gatgggcaaa ggggaaatca cccgaaaggg agtattcgcc
     9001 ccggaagctg ccgtagaccc ggaccgcttt atagatgaac tggctcaaag aaatataaaa
     9061 gttgtatgca caaagggaat ttcttaagtt ggaggtgttt accatgaaaa ttgcgattat
     9121 aagcgatacc cacggaagca ttaccgcttt caacagggcc atggaagttg ctggcccctg
     9181 tgactttatt gtccacgccg gagatatact gtaccacggt cccagaaatc ctttaccgga
     9241 aggatacaag ccccaggaac tggccaatgc cataaatgcc ttagaggttc cgttcgtagc
     9301 tgcagcaggc aactgtgacg cacctatcga ccaggtactg ctctcggttc ccatccaggc
     9361 accttatgct atgctataca tcgagaattt ta
//
LOCUS       DAONUO010000034         9328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5664_length_9328_cov_3.737086, whole genome shotgun sequence.
ACCESSION   DAONUO010000034 DAONUO010000000
VERSION     DAONUO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5664_length_9328_cov_3.737086"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK768_02085"
     CDS             complement(<1..206)
                     /locus_tag="PK768_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQA59790.1"
                     /translation="MDSKINYRLFLAGACVSTIFGFTFLFTKEALDVLEPFHLLGFRF
                     ALAALIFLIMQLCGIVKINLKGKDL"
     gene            complement(366..1700)
                     /locus_tag="PK768_02090"
     CDS             complement(366..1700)
                     /locus_tag="PK768_02090"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQA59791.1"
                     /translation="MISYEEALDFIHGLNRFGTKLGLHNITKLLELLGNPHENIKIIH
                     VAGTNGKGSTCAMIASILRSAGFKVGLYISPYLEVFNERMQVNGENISNENLARLTEK
                     VREKVFYMRENGLGSPTEFEVVTAIGFSYFKEQAVDFLVLEVGMGGRLDATNVVTPLV
                     SVITPISMDHQQYLGSTLRDISREKCGIIKQGVPVVTAPQEDEAMAVIEDTCRERNCS
                     LTKVINSSVSRSPDTISYRFVNGGIKGMTFDLYTPKTDYPGLQIGLMGRHQLDNAATA
                     VAAVEPLGNSGVNLSREAIYKGLKGARWPGRLEIMKEKPTVLIDGAHNIAGIKTLKAV
                     LEEYFYDRKKILVVGILEDKDYTEMLEELIPVADTVICTAPDNPRALSAARLAEAVSN
                     IFQKSQIKVYAQEKIEDALKLASSLAADEDMVVFAGSLYMIGHVRTLLNKQH"
     gene            complement(1842..4490)
                     /locus_tag="PK768_02095"
     CDS             complement(1842..4490)
                     /locus_tag="PK768_02095"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQA59792.1"
                     /translation="MEKNLPSVYDPKQVEDKWYEYWEKENFFYADRDPDKKPFTIVIP
                     PPNVTGQLHMGHALNNTLQDILIRTKRMQGYSTLWLPGTDHAGIATEARVKEQLAEEG
                     LSKFDLGRDKFLDRVWQWKEKYGGTIINQLKKLGSSCDWSRTRFTMDEGLSRAVREVF
                     VLLYEKGLIYRDNYIVNWCPMCKTTLSDIEVEHEEQMDKLYYVKYPYKDGTGHLTVAT
                     TRPETILGDTAVAVNPEDERFKDVIGRTLILPIVGKEIPVIADSYVDMEFGTGAVKVT
                     PGHDPNDFEMGLRHDLPVITVIGEDGFMNDKAGKYEGMDRFECRNALVEDLMSEGLME
                     KIEDLGHSVGHCYRCDTVVEPMVSKQWFVKMKPLAKPAIDVVKEGKVKFVPERFAKIY
                     LNWLENIRDWCISRQLWWGHRIPAWYCQECGATTVSREDITECSRCGSSNIERDPDVL
                     DTWFSSALWPFSTLGWPEKTPDLEYFYPTSVLVTAYDIIFFWVARMIFSGMEFMKEPP
                     FEYVVITGLVRDALGRKMSKSLGNGIDPLEVIDKYGADTLRFTLSTGNAPGNDMRFYW
                     EKVEASRNFANKIWNASRFVMMNLEGFEPDKIDIEKLTLKDRWILKRINDVTHEVTDS
                     LEKFEIGIAAQKIYDFFWSEFCDWYIEMAKIDLYGDSEEGKKRTQKVLWTVLEQALKL
                     LHPFMPFITEEIWQHIPHEGKSIMVSPWPRFKEEWNFDDTEQMELIMDAVRGIRNIRA
                     EINVPPGKKAFAVARVSSPEVMGILRDNLDIIYSLAKVSEMELIDESSAIPEKAISCV
                     IKGAEIFIPMEGLVDLDKEIARLRKERATLEKELERVNKKLNNQGFLEKAPKEVVEKE
                     EQKQRDYVEILKKVEERLKMMTDIAN"
     misc_binding    complement(4563..4771)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4831..5637)
                     /locus_tag="PK768_02100"
     CDS             complement(4831..5637)
                     /locus_tag="PK768_02100"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQA59793.1"
                     /translation="MGLLIKAFIMGVVEGLTEFLPVSSTGHMIIVGDFIDFKGESFHT
                     MFEIVIQLGAILAVIFYYRDKIIDSLRHLRPGERGFELWFKIFIAFLPSAVIGLLLDD
                     LIEEYLFSSFTVAIALIGGGFLMIFAENLFGRWGMDDMDKTDIRQALTIGVGQCLALI
                     PGMSRSASTIMGGMVAGLSTKAAAEFSFFLAIPTMIAASSYSLIKGFSGMTPIQWQAL
                     GIGFVVSFFVALLAVDRFLSFLKNHSLKFFAYYRILIGIAMVVLITSGVL"
     gene            complement(5653..6636)
                     /locus_tag="PK768_02105"
     CDS             complement(5653..6636)
                     /locus_tag="PK768_02105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HQA59794.1"
                     /translation="MKPKVLVTRNIPRDGLKELFEKCQVDYHDSNELLSTEELFERVK
                     EIDGLLCVGIRTDDELMNHAPRLKVISNYGVGYDNIDVEAATRRKIIVTNTPDVVTEA
                     TAELTFSLILAVTRRIAEADRILRSKVPFRWGPMQLVGFELVGKTLGIIGFGRIGQAV
                     ARRAKAFGMNVIYYQRTTRDKSVDSSESCYYRPLEALLKESDIVSIHVPLTKDTHHLI
                     GEEQFSMMKKGSYIINTARGPVVDEEALVKALKSGILAGAGLDVFEKEPHIHPELLEL
                     DNTVLTPHIGTSTIDTRIAMAKMTAENLIAVLEGKRPANVVNPEVYSLTEN"
     gene            complement(6652..7941)
                     /locus_tag="PK768_02110"
     CDS             complement(6652..7941)
                     /locus_tag="PK768_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HQA59795.1"
                     /translation="MKEEKLKLRRKIYRLVAEHSRTLDNLDDVYEAVLDSVSEDISRE
                     EAEFRVRAALGSFEKGKIPEEVDFPFISVIEKACKGYGDDCTCVDACETEALSKSDED
                     GRPVIEIDKCLSCGLCIVACPEGAITDKAEIAQTINLIKKHKRVYAILAPAFSGQFGP
                     DVSEEQIKSALLMLGFYDVVEVALGADMITVKEANEFLKRMQRGDEFMITSCCCPPFV
                     RLAKGFRPKIAGMVSDSVSPMIAVARFIKAEDDNAKVVFIGPCIAKKTEAKLPELKDA
                     VDCVLTFEELEAIFEAYKLEPGKIDVEMPLNDASHDGRIYAHTGGVSKAIAASIKSLN
                     PGLDVKIRHGNGIKECRQILDAIEDGRLEANFVEGMACIGGCVGGPGKLVNHEVGAAA
                     VDEFAKKSKVKEAISNEAANILVEKYKEKVELDSPKM"
     gene            complement(8177..8698)
                     /locus_tag="PK768_02115"
     CDS             complement(8177..8698)
                     /locus_tag="PK768_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HQA59796.1"
                     /translation="MKKFSTRELVNLSLLIALNIVLTRVASIRINIGAVEGVRIGFGS
                     FPIILAGLIFGPAGGGIVGAIGDIIGYYINPAGAYFPPLTLSAALTGVIPPLVLKMTK
                     RPNWSLWELIVAIGIGQFITSILLTPYLLQLAFNIPFFATLPARIISQAINVPIYAVL
                     AQRIKKKADIIFN"
     regulatory      complement(8770..8868)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            9110..>9328
                     /locus_tag="PK768_02120"
     CDS             9110..>9328
                     /locus_tag="PK768_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQA59797.1"
                     /translation="MNHSVRKISNSILDLIGMTPMVKLNRLSPENGATIVAKLESFNP
                     GGSVKDRIAFSMIQDAEDRGILKPGSTVI"
BASE COUNT         2452 a         2356 c         1776 g         2744 t
ORIGIN      
        1 aggtccttgc cttttaggtt tattttaaca atgccgcaaa gttgcataat tagaaaaatc
       61 agggctgcca gagcaaagcg aaagcccagt aagtgaaagg gctccaaaac atcaagagcc
      121 tctttggtaa acaaaaaggt aaagccgaat atagttgaaa cacaagctcc cgccaaaaat
      181 agccgataat tgattttact gtccatttac attctctcct gctatgtgcg tagttataat
      241 cctttctaaa actatactat gtttttgaca gttcagcaag attgccaatt tatatacaag
      301 aattggacgt gaaaagcaca cagttaaaca cctctgtgtg ccaaaaaatg atattcagcg
      361 tgctattaat gttgtttatt tagcaaggtt cttacgtggc caatcatata cagcgagccg
      421 gcaaagacaa ccatatcctc gtcagcggcc agagaagaag ctagcttaag ggcatcttct
      481 atcttctctt gggcataaac cttgatttga gatttttgaa agatatttga tacggcttcg
      541 gcgagccttg cagcagataa tgccctagga ttgtccggtg ccgtgcaaat cacagtgtct
      601 gctacaggta tcagttcctc cagcatttct gtatagtcct tgtcttctaa aatacctaca
      661 accaagattt tcttcctgtc atagaaatac tcttcaagga cagctttgag agtctttatg
      721 ccggcaatgt tatgggcacc atctattaaa acagttggct tttccttcat tatttccagt
      781 ctgcctggcc atctggcccc ttttaagccc ttgtatatag cttcccttga taaatttaca
      841 cccgagttac ccagcggttc cacggcagca actgcagttg cagcattgtc caactgatgc
      901 cttcccatga ggcctatttg aaggcccgga taatcagttt tcggagtata caaatcaaag
      961 gtcatgccct ttatgccgcc atttacgaac cggtagctta tggtatcagg tgatctgctt
     1021 acactgctat ttatcacctt tgtaagtgaa cagtttcttt ccctgcaggt atcttcaatg
     1081 acagccatag cttcatcttc ctgaggagct gtcaccacag gaaccccctg cttgataatg
     1141 ccgcattttt cccgggaaat atcgcgaagg gtgctgccca ggtactgctg atggtccatg
     1201 cttatcggcg ttatgacact taccaggggt gtcaccacat ttgtggcatc aagcctgccc
     1261 cccatcccca cttcaaggac gaggaaatcc accgcctgtt ctttaaaata ggaaaagccg
     1321 atagccgtca caacttcaaa ttccgtcggt gagcccagac cattttccct catgtaaaac
     1381 accttttcac ggaccttttc ggttagcctt gccaaattct cattgctgat attttctccg
     1441 ttgacctgca tacgctcatt gaaaacttcc aggtacgggg atatataaag gccaacttta
     1501 aaacctgcag accgaagaat tgaagcgatc atggcacagg ttgagccttt gccgttggtg
     1561 ccggccacat gaattatttt tatattctcg tgtggattgc ctaagagctc tagaagtttt
     1621 gtgatattgt gaaggcccag ctttgtgccg aatctattaa gaccgtggat aaaatctaag
     1681 gcttcctcgt agcttatcat atgttctttc ccttctatag aatatttatg acagaataga
     1741 taaagatgac ggcaattctc cccaaaggaa agcccttact caacctggtt ttgccgccac
     1801 cttcatccga aaaacatttc ctttatgcgt atttaacttg tttaattggc tatatcggtc
     1861 atcatcttta gacgttcttc aacctttttc aatatctcaa cgtagtccct ttgtttctgt
     1921 tcttccttct ccactacttc tttgggtgcc ttttctaaga atccttgatt gttcagtttc
     1981 ttatttaccc tttcaagctc tttttctaat gtggctcttt ccttcctgag tctggctatt
     2041 tccttatcca aatccaccaa gccttccatg ggtataaaga tttcggcgcc cttgattacg
     2101 caggaaattg ctttttccgg tatagctgag ctttcgtcaa ttagctccat ttcggagact
     2161 tttgccaagc tgtaaataat atcaagattg tcccttagta tgcccattac ttccgggctg
     2221 ctgacccttg caacggcaaa tgctttcttt ccgggaggaa catttatttc cgcacggata
     2281 ttcctgatac cccgcaccgc atccattatg agttccattt gctctgtatc gtcgaagttc
     2341 cattcctctt taaatcgtgg ccagggagat accatgatgc ttttgccttc atgagggatg
     2401 tgctgccata tttcctcggt gataaagggc ataaagggat gtagcagttt taacgcttgc
     2461 tcaaggaccg tccacaacac cttttgtgtc cgcttcttac cctcttcaga atcaccgtat
     2521 aggtctatct tggccatctc tatataccaa tcgcagaatt cgctccagaa aaagtcgtag
     2581 attttctggg cagcgatgcc gatctcaaat ttctccaggg aatcagtaac ttcgtgggtt
     2641 acgtcattga tgcgctttag tatccagcgg tcctttaaag tcagcttctc tatatcgatt
     2701 ttatcaggct caaagccctc caaattcatc atcacaaagc gggaagcgtt ccatatcttg
     2761 tttgcaaaat tgcggctggc ttcgaccttc tcccagtaga atctcatatc gttgccgggg
     2821 gcattgccgg tgctcaaggt aaaccgcaaa gtgtctgccc cgtacttgtc tataacctcc
     2881 aaggggtcga taccgttgcc aagggacttg ctcatcttgc gccccagggc atcgcgcaca
     2941 aggcccgtga tgacaacata ttcaaagggc ggctctttca tgaactccat gcctgaaaag
     3001 atcatcctgg caacccagaa gaaaattatg tcgtaggcag taaccagcac tgacgtgggg
     3061 taaaaatact ccagatccgg cgttttttcc ggccagccca gagtggaaaa tggccatagg
     3121 gccgaactga accaggtatc cagcacatcc ggatcacgct cgatattgct gctgccgcac
     3181 cgggaacact ccgttatatc ttcacgagac acggtagttg ctccgcattc ctggcaatac
     3241 caggcgggaa tcctgtggcc ccaccaaagc tgcctggata tgcaccaatc ccggatattt
     3301 tcaagccagt ttaagtaaat ttttgcaaac ctttcaggga caaactttac cttcccctct
     3361 ttcaccacgt caatggcagg ctttgcaagg ggtttcatct tgacaaacca ctgttttgaa
     3421 accatgggtt ccacaacggt atcgcagcga tagcagtggc cgacgctgtg gcctaaatcc
     3481 tcaatcttct ccatcaggcc ttcactcata aggtcttcca ccaaggcatt cctgcactca
     3541 aacctatcca taccttcgta ttttcctgcc ttatcattca taaatccatc ttcacctata
     3601 accgtaatta ccggcagatc gtgccttagg cccatctcaa aatcgttcgg atcgtgaccg
     3661 ggtgtaactt ttacagcacc ggtaccaaat tccatatcta cataagaatc cgcaatgacg
     3721 ggaatttcct tgcccactat tggcaggatt agggtcctgc ctatcacatc tttgaagcgc
     3781 tcatcttcag gatttacggc taccgccgta tctcccagaa tagtctctgg ccttgttgtt
     3841 gccaccgtca aatggccggt tccgtccttg tagggatatt ttacatagta taacttgtcc
     3901 atttgttctt catgttccac ttctatatcg gaaagggtgg ttttacacat tggacaccaa
     3961 tttacaatgt agttgtcccg atatatgagg cctttttcgt acagcaggac aaatacctct
     4021 cttaccgccc tactcaatcc ttcatccatg gtaaacctag ttctcgacca gtcacaggaa
     4081 gagcctaatt tttttaattg attaataatg gttcccccgt atttttcctt ccactgccaa
     4141 accctatcta aaaacttgtc cctgcccaga tcgaatttgg acaagccttc ttcggccaac
     4201 tgctccttta ctctggcttc ggtagcaatc ccggcatggt cggttccagg aagccacaag
     4261 gtactataac cctgcatgcg cttggtccgt atcaagatat cctgcaaggt gttgttcagg
     4321 gcatggccca tgtggagctg gcctgttaca tttggcgggg ggataactat ggtaaagggc
     4381 ttcttgtcag gatccctatc tgcataaaag aaattctctt tctcccagta ttcataccac
     4441 ttgtcctcaa cctgttttgg atcgtaaaca gatggcaaat ttttttccat ttaatccact
     4501 cctatactta aaagcttaat atttttattt tccgtaatga atctaaaaaa gtctttcatc
     4561 cactaaagga cgaaagactt tcttccgcgg taccacctta attctccata cagagcactt
     4621 tagctttaac gggcatgccg tctccgacta cttaagttca ccggagctgc tccggggcga
     4681 ccttcaaagc tactcctgga aggctttcag ccgggacctt cctctctttt taaaggagct
     4741 cgcttttact cctccccttc accgcattct tttttactat tctacctatg acacaggcct
     4801 ttgtcaagat ttgctgaagc tgctgaaatt ttatagcaca cccgaggtaa tcagcacaac
     4861 catagctatc ccaattaata tgcgatagta ggcaaagaat ttaagagagt gattttttaa
     4921 aaatgacaaa aacctgtcta ctgccagcag cgcaacaaaa aaagaaacta caaatccaat
     4981 tcccagggcc tgccattgga taggagtcat gccggaaaaa cctttaatga gtgagtatga
     5041 gcttgccgca atcatggtag ggatggcaag gaaaaatgaa aactccgctg cagcttttgt
     5101 agaaagccct gctaccatac ctcccataat ggtagatgcg gaccttgaca ttcccggtat
     5161 caaagcaaga cactgaccga cacctattgt taatgcctgc ctgatatcgg tcttatccat
     5221 atcatccatg ccccagcggc cgaataaatt ctcggcaaat atcattaaaa atcctcctcc
     5281 aattagagca attgccacgg taaaggagga aaaaagatat tcttctataa ggtcgtcaag
     5341 caataagcct attaccgctg atggcaaaaa cgcaatgaat attttaaacc acaattcaaa
     5401 gcctctttca cctggccgca agtgtcgtaa agaatctatt attttgtccc tgtagtagaa
     5461 aatgacggct aagattgcac ccagctgtat gacgatttca aacatggtgt ggaaactttc
     5521 gcctttaaaa tctatgaaat caccaacgat aatcatgtgg cccgtagagg atacgggtaa
     5581 aaattcggtg agaccctcca ctactcccat aataaaagct ttgataagta agcccatgct
     5641 agttctcctt ctttagtttt ctgtcaaact ataaacttca ggatttacta catttgctgg
     5701 tcgctttcct tcaaggactg ctatgagatt ttcagctgtc attttagcca tggcaatcct
     5761 ggtatctatg gtgctggtcc cgatatgcgg agtcaacacg gtattgtcaa gttctaaaag
     5821 ttccggatgt atgtggggtt ccttttcaaa tacgtctaaa cccgcccctg ccaaaatgcc
     5881 gctctttagg gcttttacta aggcttcttc atctacgacg ggacccctgg cagtatttat
     5941 tatataactg ccttttttca tcatggagaa ctgttcttct ccaatcaggt gatgagtgtc
     6001 tttcgtcagg ggcacatgaa tgctaactat atccgactcc ttaagcaatg cctccaaggg
     6061 tctgtaataa cagctttcgg atgagtcaac acttttgtcc cgcgttgtcc tctgatagta
     6121 tattacattc atgccgaaag ccttagccct gcgtgcaaca gcctgaccta tgcggccaaa
     6181 accgatgatt cccaaggtct tgcccaccag ttcaaagccg accaactgca ttggccccca
     6241 tcgaaaaggc accttgcttc tcaaaatgcg gtctgcttcg gctatccggc gagtcaccgc
     6301 taaaataagg gaaaaggtca attccgcagt cgcttcggtg accacatccg gtgtattcgt
     6361 aactataatt ttgcgtcgtg tggcagcttc cacatctata ttgtcatatc ccactccgta
     6421 attgctaatc accttcagcc ttggagcgtg attcatcagc tcatcatctg ttctgatgcc
     6481 tacacacaaa aggccgtcaa tctctttaac tctctcaaac aactcttcag tagacaagag
     6541 ctcgttagaa tcgtggtaat ccacctggca tttttcaaaa agctctttca ggccatcacg
     6601 aggaatattt cttgtcacaa gtacctttgg cttcaacccc tatacctccc tttacatttt
     6661 tggagaatca agctcgacct tttctttgta cttctcaaca agaatattag ctgcttcatt
     6721 tgatatggct tcctttacct tgctcttttt tgcaaattca tcaactgctg ctgcaccaac
     6781 ttcatggttc accagttttc ccggtcctcc tacgcagcca ccgatacagg ccattccttc
     6841 aacaaagttg gcctccaatc tcccatcctc tattgcatcg agaatctgcc tgcactcctt
     6901 tatgccattg ccatggcgta ttttcacatc aagacccgga tttaaactct tgatgctggc
     6961 ggcaatcgct tttgacacac ctccggtatg ggcatatatt ctgccatcgt gggaggcatc
     7021 atttaacggc atttctacat cgattttgcc gggttccagc ttgtaagcct caaagattgc
     7081 ctccaactct tcaaaggtca gtacgcaatc gacagcatcc tttaattcgg gaagttttgc
     7141 ctccgtcttt ttagctatgc aaggccctat aaagaccact ttggcgttgt catcttcggc
     7201 cttaatgaac ctcgcaacag ctatcatggg agataccgaa tccgaaacca tgccggctat
     7261 cttcggcctg aagccttttg ccaggcggac aaatggtggg caacaacagg aggtaatcat
     7321 aaactcatcg cctctctgca tgcgctttaa aaactcattg gcttccttca ctgttatcat
     7381 atcagctccc agggcaacct ctaccacatc ataaaagcca agcatcaaaa gggcggattt
     7441 tatctgttcc tcacttacat cggggccaaa ttggccggaa aatgccggtg ccaaaatggc
     7501 atataccctc ttgtgctttt taattaaatt tatggtctgg gcaatttctg ccttgtcagt
     7561 aatggctccc tcgggacagg ctactatgca taggccgcag gaaaggcact tgtcaatttc
     7621 tataaccggc cttccgtctt catcgctttt ggaaagtgct tcagtctcgc aggcatccac
     7681 acacgtacaa tcatcgccat atcctttaca tgctttttca attacggaaa taaaggggaa
     7741 atctacttct tccggaatct tccccttctc aaaagaccct aaggctgccc ttaccctaaa
     7801 ctcggcttct tccctgctta tatcttcgct tacactgtcg agtaccgctt catacacatc
     7861 atccaaattg tccaaggtcc gggaatgttc tgcaaccaac ctataaatct ttcgcctgag
     7921 ttttagtttt tcctctttca tgatgacctc ccgctcttat actcatatgt aaactacaca
     7981 ttatatatat agtatagtat atatcttgcg gtaaataaag gcatcacgcg aatttcccat
     8041 gacatcagcc tcgtataaaa tggtatggct gtgcttcccg ttgaaccgcc atccaactca
     8101 agttgaagga tagtgatttt gcacatacag gatgcatcat tgaaaagagc ccctatccgg
     8161 ggctccaact acggtcttaa ttaaatatta tatctgcttt ctttttgatt ctttgggcca
     8221 aaactgcata tattggcacg ttaatagctt gtgatattat tctcgcgggc aatgtagcga
     8281 aaaatggtat attaaaggcc agctgcaaca aatacggtgt taaaaggata gaagtgatga
     8341 attggccgat acctatagca acaataagct cccacaaact ccaattcgga cgttttgtca
     8401 tctttaaaac tagaggtggt attacgccgg ttaaggcagc cgataaagtt aatggaggga
     8461 aataggctcc cgccggattt atataatacc cgattatgtc gcctattgct cctacaattc
     8521 ccccacctgc aggtccaaaa ataaggcctg ctaaaattat gggaaagctt ccaaaaccga
     8581 ttctcacgcc ctcaactgct ccgatgttta ttcttatgct ggcaactctt gtaaggacga
     8641 tgttgagagc aattaacaag gacaaattga ccagctctct ggtagaaaac tttttcatta
     8701 aaacaagcct ccttccatta ttaatgggca gaatctgcca caccaatgga aaaaggcttc
     8761 ctctgatgtg gcagcgaacg cgataactta ccgcaagctt ttaaaaagct tttcgtctaa
     8821 gggcaacgtc ccatccctta gcacttaacg caagttacct actctgccat tttcttttga
     8881 tatgtattct atcaaagttt tcccctcttg gctagtgttt ttttactttt ttagacaaat
     8941 tgaccgcttt tgcagaaaaa aatgaaggca cgtcccccgc ttctattatc attgtatgtg
     9001 taaccctata attatcataa aaaaattcaa ttttttaaaa agtttttatg gaaaaaataa
     9061 gccaggtact atatactata aaatcaaaag ctacaaagga ggtttcaccg tgaaccactc
     9121 tgtccgtaaa atttccaatt cgattttaga tttaatcgga atgaccccta tggttaaact
     9181 taatcggcta tctcccgaaa atggagcaac aattgtcgca aagcttgaat catttaatcc
     9241 cggcggtagc gtaaaggacc gcatagcttt tagcatgata caagacgccg aagacagagg
     9301 tatccttaaa cctggctcta cggttatt
//
LOCUS       DAONUO010000035         9165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5799_length_9165_cov_3.601098, whole genome shotgun sequence.
ACCESSION   DAONUO010000035 DAONUO010000000
VERSION     DAONUO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5799_length_9165_cov_3.601098"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(509..1852)
                     /locus_tag="PK768_02125"
     CDS             complement(509..1852)
                     /locus_tag="PK768_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HQA59798.1"
                     /translation="MALLVSIILLTLLGLGLRSIKPVTSDIYARDGFAIVALGWLFVS
                     VFGGLPFLISGAIPSVMDALFESASAFSTTGASILKEVEGLPKGILFWRSSAHWMGGM
                     GVLILMIAVLPSVKANTLYIMKAETPGPVPEKFVPKVGQTAKILYVIYIAMTAIEVIF
                     LLAGGMSLYDALIHAFGTASTGGFSNRNASIGAYNSVYIDAVVTIFMLLFGVNFTVYY
                     ALLKVNVKSILRDEELKFYLGTVITATVLIILNTYGTVFQTIDEAIRYSSFQVSSIIT
                     TTGYSTADFALWPVFSQSILVLLMFLGASAGSTGGGMKCLRIILLFKIIKREIIKIIH
                     PRSVQTVKINGRVVDEEILSGVMAFFFAYVAIFAAATIIVSLDNKDMISTATAVASCL
                     SNIGPGLGVVGPTGNYADFSDISKAVLSLCMIIGRLEIYPILLLFTPTFWKRLNI"
     gene            complement(1938..3326)
                     /gene="trkA"
                     /locus_tag="PK768_02130"
     CDS             complement(1938..3326)
                     /gene="trkA"
                     /locus_tag="PK768_02130"
                     /inference="COORDINATES: protein motif:HMM:NF007041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HQA59799.1"
                     /translation="MKIIIIGDGKVGYSLAENLSKENHDVTIIDKNSEALKKAEENLD
                     VMCIRGNGVSTKILMMAGVQDTDLLIAVTESDEMNMVCCLTAKKLGVGHTIARIRDPE
                     YASELSLLKRELGLNMVINPEQAAGSEIARLIKFPPATNVEIFAKGRVEMVEINVTND
                     MAISNMQLKNIANRFSSSVLIGAVQRGDDIIIPDGEFTVREGDTIYIFGQSAKVFDFC
                     RRIGIHVQKIKNVMIMGGGRIAYYLAKYLDEMNIHVKIIEIDHERCRELTELLPHALI
                     ICGDGSDETLLKSENFSEMGAFVAVTGRDEDNIIAALLAKQFGIGKVIAKINRMNHSD
                     IIKSMGIDNVVSPKLITTNYILRFVRGLQNAMGNHVNALYRIVEDKAEAIELTAGNSS
                     KYIDIPLKNLNVKKGILIAAIVRKNEIIIPNGNDVIKPGDNVILITKDYKITDLENII
                     DVVKKHELWNDF"
     gene            complement(3363..3692)
                     /locus_tag="PK768_02135"
     CDS             complement(3363..3692)
                     /locus_tag="PK768_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59800.1"
                     /translation="MKQIDKSDSQIELEKLCNTVRTLIEHKEFKKCELLISKAMEKYP
                     HAPQPHNLIGILLEMEGDHLTAMKHFRAAWALDPSYIPARYNMELFLSFSPKGQFAFD
                     ETDCPIE"
     gene            complement(3830..4528)
                     /locus_tag="PK768_02140"
     CDS             complement(3830..4528)
                     /locus_tag="PK768_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA59801.1"
                     /translation="MSYYNEYILVVEDDIKIRNYICYSLQTEGFRYITASTGQGALKT
                     LVSEPIDLLLLDLGLPDIDGMEIIKKIREWSEMPIIVVSARDQDKEKAAALDLGADDY
                     LTKPFSSTELLARIRVALRHFYKQGGNKTPAIIQVGGLRIDFEKRLVYLDDKELHVTP
                     LEYKLLSLFFKNVGKVLTTKYILKEIWGIDYSSDTQVLRALMAGLRRKIEKNPAKPRY
                     IVTEVGVGYRLVDE"
     gene            complement(4515..6080)
                     /locus_tag="PK768_02145"
     CDS             complement(4515..6080)
                     /locus_tag="PK768_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HQA59802.1"
                     /translation="MGEINNNQAIDAGHQNRNRKDTLYCLFIMMILLISATAIGFVFR
                     TVEFPETNIVLVYLLAVLLTARMTHGYIFGFLACVIATGAFNFFFAEPYLAFSVEASN
                     YIVTFVVMTITALITSTLTSYGKQKTIEANKREAETKALYSLTHRLSGAADMDDIASI
                     AISTISDIMSTQAACLCFDEHGMPMSTFIQQVSSEKQIRRDYTDTPDIKCQIQKINTG
                     YIKGVEFYDWPIYGSETILGIIRIPKERAETMNESQMRLLKTMIESIALAMDRYWSIN
                     HRIRTNEEIMRERYRGNLLRAISHDLRTPLSGIIGTAEMLMHMIQRNDTLYSLAEDIY
                     KEALWLHSLVENILNLTRLQDGKLVISKDMEAVEEVVGSAVGHILRRYPEREIEVSVP
                     DEFLMVPMDAKLIEQVLINLLENAVKHTLPEQEISVSVTEDKRNDCVVFTVADRGEGI
                     DPVDLPNIFQMFYTSNLKHADSHYGIGLGLAICDAIIKAHGGSIVARNRTDGQGAMVI
                     FKLPMEMNQNELL"
     gene            complement(6871..8115)
                     /gene="codA"
                     /locus_tag="PK768_02150"
     CDS             complement(6871..8115)
                     /gene="codA"
                     /locus_tag="PK768_02150"
                     /inference="COORDINATES: protein motif:HMM:NF005748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HQA59803.1"
                     /translation="MLFRNGLIENATEGVDIRVLDGKFAAIAPNLEPLPGEEIIDCTG
                     KLILPPFIESHIHLDTCLTAGQPSWNMSGTLFEGIERWEERKQFLTKEDVRSRVKKVL
                     HMQVANGIQHVRTHVDINDPKFTALEAILELREEVKSFINIQIVAFPQYGILSYPKGR
                     ELLEEALKMGADAVGAIPHFEFTREYSVESLNICFELAQKYGKLIDVHCDEIDDEASR
                     GLETLAARAYETGMRDMVTASHTTAMHSYNNAYVNRLMRLLKMSGINFVANPCVNVHL
                     GGRVDNYPKRRAMTRVKELVAEGINVSFGNDDIFDPWNPMGNAGMRDAVFMGLYVGHM
                     LGYEEIMKSYKFVTYNAARTLHLGDAYGIKEGNSASFVIMDAKNYYEVLKDNATVLAS
                     YKDGKLIARTEPAKTQVFVPGE"
     gene            complement(8169..8381)
                     /locus_tag="PK768_02155"
     CDS             complement(8169..8381)
                     /locus_tag="PK768_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59804.1"
                     /translation="MNNKKNNIPFEDELLAGIVGGMAVGVDCGLQENNFPNWKVLSDE
                     QFHCRSGLSGGLPLLQRTRTPHFLLQ"
     gene            8487..8714
                     /locus_tag="PK768_02160"
     CDS             8487..8714
                     /locus_tag="PK768_02160"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HQA59805.1"
                     /translation="MGKVIDLTKSVYEICKKHPEVVDIMKDLGFENITSPGMLNTAGR
                     FMTIPKGAAMKGIPMEKIKEAFAAKGFDLES"
     gene            complement(8722..8838)
                     /locus_tag="PK768_02165"
                     /pseudo
     CDS             complement(8722..8838)
                     /locus_tag="PK768_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8902..>9165)
                     /locus_tag="PK768_02170"
     CDS             complement(8902..>9165)
                     /locus_tag="PK768_02170"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HQA59806.1"
                     /translation="VMVFTLLGDAYIGGELVKEKTAVKLTSGDQVEIKATDKKAQVLF
                     ISSIRLDEPIVWGGPIVMNTKEELNKAFDDLKKGTFIQKIISY"
BASE COUNT         2685 a         2062 c         1613 g         2805 t
ORIGIN      
        1 tacatgaggt ttgctcttgg tatgtgaaaa tctaagaata ggtgagatat ttaaacataa
       61 ataaagatta tagagggagg gtgaagtaga acctaaaacc tatctgacca gtacaaataa
      121 cagaatcacc actcatttat gtttccaggg gtttgctgca tgtttcgcta ctcagatgca
      181 tttgtacaac ataaaggaag gtatccctga caatataaac attttccccg tctatcacca
      241 cggggaaccg ttgactcagc gactctctct tatctggtgt aaggaccgat atttgccccg
      301 ctacgccaaa tacttcctag attgtctgtt ccgatttttt aacaatgtgg atcaaatcca
      361 tatgagtcga aaggtttaat ccttgcgata aaaaatccac tagttctacc ttatatgcga
      421 aaggcttttt ttcctccttg ctataatgct ttcttacagg taatatctat aaaaaatccc
      481 gctttagaag cgggtataca gccataaatt aaatatttaa gcgtttccag aaagtggggg
      541 taaagagtaa aagtatcgga tatatttcca gccttccgat aatcatgcat aaagataaca
      601 ccgctttgct aatatctgag aaatcagcat aatttcctgt cgggccaact actcccaatc
      661 ccggcccaat attgctcaaa catgaagcga ctgccgttgc ggtagaaatc atgtctttgt
      721 tatctaatga tacaataata gtggctgcag caaatattgc aacataagca aagaaaaatg
      781 ccattactcc ggatagtatt tcttcatcaa caactctccc attgatttta acagtttgca
      841 cagatcttgg atgaataatt tttatgattt cccgcttgat aatcttaaaa agcagtatta
      901 tccgaaggca tttcatccct ccacctgtgg accctgcact tgctccaaga aacatcaata
      961 agacaagtat gctttggctg aatacgggcc aaagcgcaaa gtccgcagtt gaataaccgg
     1021 tcgttgttat tatggaactt acctgaaaag acgaatatct tatagcttca tctattgttt
     1081 gaaaaaccgt gccataagta tttaatataa tcaatacagt agcagttatc acagttccta
     1141 aatagaactt cagttcttca tcccgcagga ttgactttac attaactttc aataatgcat
     1201 aatatacagt gaaattaaca ccaaataata acataaaaat ggtgactaca gcatctatat
     1261 aaacgctgtt gtatgcacct atacttgcat ttctgttgga aaagccgcca gtactcgctg
     1321 ttccgaaagc atgaatcaaa gcgtcataca gggacattcc tcccgcaagt aaaaatataa
     1381 cttcaatggc agtcatggca atataaatga catacaggat tttggctgtt tgcccaactt
     1441 taggaacaaa tttttcaggg acaggtcccg gagtttcagc cttcattata taaagtgtat
     1501 tggctttcac agaaggcagg acggcgatca ttagaatcaa gactcccatt ccgcccatcc
     1561 agtgtgcaga acttctccaa aacaaaatgc ctttaggtag tccctccact tcctttaata
     1621 tactagctcc agtagtgctg aacgctgatg cagactcaaa taaagcatcc atcacagagg
     1681 gaatggctcc acttataaga aacggcaatc ctccaaacac agaaacaaat aaccaaccta
     1741 aagctacaat tgcgaaacca tcccgcgcat aaatgtcact cgttaccggc ttaatggagc
     1801 gaagtcccaa tcccaataac gttagtagaa ttatagaaac aagcaaggcc attgtgtccc
     1861 cttcccaata aataaacgat atcaacaggg atggcaccat acatgcggcc tcaacgcata
     1921 acacgcctcc taagctctta aaaatcattc catagttcat gctttttcac cacatcaata
     1981 atgttttcta aatctgttat tttataatct tttgtaataa ggataacatt atcacctggt
     2041 tttatcacat cattgccatt tggaattatg atctcatttt ttctaacaat agcagcaatt
     2101 aaaatccctt tttttacatt gagatttttc aggggtatat caatatattt gctggaatta
     2161 ccggcagtta gttcgatggc ttctgcctta tcctcaacaa tacgatataa tgcgtttacg
     2221 tgattaccca ttgcattttg caatcctctt acaaatctca aaatataatt tgtagtgatg
     2281 agctttggac tcaccacatt atcaatgccc atacttttta taatatctga atgattcatc
     2341 ctgttaattt tggctataac tttcccaata ccgaattgtt ttgccaataa tgcagctatg
     2401 atattatctt catctcgtcc cgtgacggcc acaaaagcac ccatttcact gaaattttca
     2461 gactttaata atgtttcatc agaaccatca ccgcagataa tcaaagcatg aggcagaagt
     2521 tccgttaatt ccctacacct ttcgtgatcg atttctataa tttttacatg aatattcatt
     2581 tcatccaaat attttgccaa ataatatgca atgcgtcctc cgcccataat catgacattt
     2641 tttatctttt ggacatgaat acctatgcgt ctgcaaaagt caaaaacttt cgcggattga
     2701 ccaaatatat atattgtatc accttccctg actgtaaact caccatcagg gataataata
     2761 tcgtctccac gttgcacggc acctatcaga actgatgaag aaaatcggtt tgcgatattc
     2821 tttaattgca tgttagaaat tgccatgtca ttggttacat ttatttccac catttcgact
     2881 ctacccttgg cgaatatctc aacgttggtg gcaggcggaa acttaatgag gcgggcaatt
     2941 tcacttccag ctgcctgttc aggattgata accatattta agcccagctc tcttttcaat
     3001 agagaaagtt cgctggcata ttcagggtct cttattcggg caatcgtatg ccctacacca
     3061 agttttttgg cagtaagaca gcataccata ttcatttcat cactttcagt tacagcaata
     3121 agaaggtcgg tatcttgcac cccagccatc ataagtattt ttgtgctgac gccatttcct
     3181 ctaatacaca ttacatccaa attttcttcg gcctttttta atgcttctga gtttttatct
     3241 ataattgtaa catcatggtt ttccttggat aagttttcag ctaaactata gcctactttc
     3301 ccatcaccaa taatgataat tttcattatt aatccccctt acaatagcct tttcagttac
     3361 ttttattcta tgggacagtc tgtttcatca aatgcgaatt ggccttttgg agaaaagctt
     3421 aaaaaaagtt ccatgttata ccgtgcagga atatagcttg gatcgagggc ccaggccgcg
     3481 cgaaaatgtt tcatggcagt caaatggtca ccttccattt caagcaatat gccaatcaaa
     3541 ttatgaggct gtggggcgtg aggatatttt tccatagctt tcgatataag aagttcacat
     3601 tttttgaact ctttatgttc aataagcgtt ctcacagtat tacacagttt ttccaattct
     3661 atctgactat cagatttgtc tatttgtttc attttttacc cccctcatta agatttctta
     3721 ctttacaatg atagcaaaag cttcgtgaga atagtgtgaa aatgctgtta gaatgctgtg
     3781 agaaaaaata ataaaaccgt atcgctacgg tttttcacaa ttttattctc tattcatcta
     3841 caagcctgta tccaacccct acttctgtca ctatatagcg aggtttggcg ggattcttct
     3901 caatttttct tctaagcccc gccattagag ctcgcaatac ttgagtgtca ctgctataat
     3961 ctatgcccca aatctctttt aagatatatt tcgtagtaag caccttgccg acatttttaa
     4021 aaaacagtga caatagctta tattccaaag gtgtcacatg cagctcttta tcatctagat
     4081 aaactaaccg tttttcaaag tctatccgca aaccaccaac ttgaatgatt gccggtgtct
     4141 tatttcctcc ttgtttataa aaatggcgca gagcaacacg aattcgagcc aaaagctctg
     4201 ttgaagaaaa gggctttgtt agataatcat ccgcacctaa atcgagggca gctgcctttt
     4261 ccttgtcctg gtcacgggca gacacaacaa taatcggcat ttctgaccat tctcgaatct
     4321 tctttataat ctccatgcca tcaatatcag gtaatcccaa atccagcagc aataaatcga
     4381 taggttctga tacaagagtt ttcaacgctc cttgaccggt gcttgcagta atatatctga
     4441 acccttccgt ctgcaatgaa taacagatgt aatttcttat ctttatatca tcttcaacaa
     4501 caaggatgta ctcgttataa tagctcattt tgattcatct ccattggaag cttaaaaata
     4561 accattgccc cttggccatc tgtacggttg cgagccacta tactgcctcc atgcgctttt
     4621 atgattgcat cacatatcgc aagccccaag ccaataccgt aatgtgaatc agcgtgttta
     4681 agattggaag tatagaacat ttgaaatata tttggcaaat cgactggatc tattccttcg
     4741 cctcgatccg ctacggtgaa cacaacacaa tcatttcgtt tatcctctgt tactgatacg
     4801 ctgatttctt gctctgggag agtatgctta accgcattct ccaaaaggtt tattaaaact
     4861 tgttcgatta gctttgcatc catagggacc atcaggaact cgtccggcac actgacctct
     4921 atttcgcgtt ccgggtatct gcgtaaaata tgcccaacag cactgcctac cacttcttca
     4981 acggcttcca tatctttgct tattactagt ttcccatcct gcaaacgggt caggttcaaa
     5041 atgttttcaa ccaatgagtg aagccataag gcttctttat atatatcttc tgcaagacta
     5101 taaagtgtat catttctctg tatcatatgc ataagcattt cggctgttcc aataatgccg
     5161 gaaagtggag tacgcaaatc atgagatatc gcacgcaaca agtttcctcg ataacgctcc
     5221 cgcatgattt cttcattcgt tcgaatacga tggtttattg accagtaacg atccatcgcc
     5281 aatgcaatgc tttcaatcat agttttaagc aaccgcatct gcgattcgtt cattgtttct
     5341 gctctctctt taggtatacg gatgatacct aatatagttt cactgccata gataggccaa
     5401 tcataaaatt caacaccctt tatatagcct gtattaatct tttgtatttg gcatttaata
     5461 tcaggcgtat cagtataatc acgtcgaatc tgcttttcag atgatacctg ctgaataaag
     5521 gtactcattg gcatgccatg ttcatcaaaa caaaggcatg ctgcctgtgt gctcatgata
     5581 tcgcttattg tactgatagc aatgctagca atgtcatcca tgtctgccgc tccggaaagc
     5641 ctgtgtgtta gtgaatatag tgcttttgtt tccgcttctc ttttattcgc ttcaatagtt
     5701 ttttgcttgc cataagatgt aagagtgctt gttataagcg ctgttatagt catgacgaca
     5761 aaagtaacga tataatttga agcttcaaca gaaaatgcaa gatatggctc ggcaaaaaag
     5821 aaattaaacg caccggttgc tatcacacag gctaaaaaac caaagatata tccatgggtc
     5881 atacgagccg ttaacaatac tgcaagcaga tagacaagga caatattggt ttcaggaaat
     5941 tcgacagtac gaaacacaaa acctatggcc gttgcagaaa ttaatagtat catcattata
     6001 aacaggcaat ataatgtatc cttcctgttt ctattttgat gtcccgcatc gattgcttga
     6061 ttattgttta tctcacccat agttttgttc ctctctcgta atcatcataa atcaacccta
     6121 ctcaattgtc gaggaaaaca agccctgtcc tctctttttt tgtgtactat tctagcacat
     6181 attggcattt tataaaacag tcatcgtgga acttttgtat aagatgtcaa taaatgcgaa
     6241 gagacctttt tgaaggcttt attctatatg tgttagtcct tttgttagcc cgtacacaaa
     6301 ccaggggaca tatacattgt attattgagg ttaaataatc tatcttgtgc cccataacct
     6361 gggcataaag gctgcttcca ccggataaca aagccgcaaa ggccatattg tattcaggca
     6421 ggactatcat gcttccatga tataagatag tatcgccgcc ctttattaaa gactggatac
     6481 catattgact gtacgggtat gcttccaccg aatcccggcc caagccatgg ccgaaaagcc
     6541 cttcctcctg gacattattc cacaatttga caaataaaaa tctgtgtaac ttgataaagt
     6601 agaaaaataa atacggaagc attttataaa caaaaacagg ataccatccc ctaatttaag
     6661 gaatagtatc ctgtctatgt tctacagtag tttgagcatt tccagcgccc agtctacctt
     6721 cgttttttca ctcgtgacac cgcccaatac gacgacatta caattctggt tctgactctt
     6781 aaaagtttgc agaaataggc gttctgccca caagcatgat atttttgcaa aggtgaatca
     6841 cgctaaatta gattactatg gggacaagtt ctattcgcca gggacgaata cctgagtctt
     6901 ggcgggctct gtcctggcta taagcttgcc gtccttgtag gaagccagaa cagttgcgtt
     6961 gtctttcagc acctcgtaat agttctttgc atccatgata acaaagctgg cgctattgcc
     7021 ttccttgatt ccataagcat cacccaagtg aagagtacgg gcagcattgt aggtaacaaa
     7081 cttataggat ttcatgattt cctcataacc cagcatatgc cccacataca ggcccataaa
     7141 caccgcatca cgcattccgg cattgcccat tggattccaa gggtcgaaga tatcatcgtt
     7201 gccaaatgac acgttgatac cctctgcgac caattccttc acacgggtca tggctctgcg
     7261 cttaggatag ttatccacgc ggccacctag atggacattg acacagggat ttgccacgaa
     7321 gttgataccg gacatcttca gtaaacgcat gagacggttc acataagcat tattgtagct
     7381 gtgcatggca gtagtatgtg aagcagtgac catatccctc atacctgttt cataagctcg
     7441 ggcagccagt gtctccagac cacgggatgc ttcatcatcg atctcgtcac aatggacatc
     7501 gatgagcttg ccatacttct gggccagttc gaagcagata tttaaggatt ccacgctgta
     7561 ttcccgggtg aactcaaagt gaggaattgc accaacagca tcggccccca tcttcagggc
     7621 ctcttccagt agctctctac ctttaggata gctgagaatg ccgtactgtg gaaaggctac
     7681 aatctgaatg tttatgaagc tttttacttc ctccctgagt tccagaatgg cttccagtgc
     7741 ggtaaacttt gggtcgttga tgtccacatg ggtacggaca tgctgaatac cgttagcaac
     7801 ctgcatatgg agcacctttt tgacacgtga ccttacatct tcctttgtca aaaattgttt
     7861 tcgctcctcc caacgttcta tgccctcgaa gagggtgccg ctcatgttcc aactgggctg
     7921 accggcggtg aggcaagtgt ccagatgaat gtgggattcg ataaaggggg gcagaatcaa
     7981 cttgccggtg cagtcaatga tttcttcacc tggcagaggc tccaggtttg gagcgatggc
     8041 ggcaaactta ccatccagca cccgaatatc tacaccttcg gtggcatttt cgatgagacc
     8101 atttctaaat aacatatttt ataccccctt tgcattaata aggaaaagct gaagaatgtg
     8161 tggtgattct attgtaacaa aaagtgagga gttcttgtcc gctgcaataa gggcaagcct
     8221 cctgaaagac cactcctgca atggaactgc tcatctgaaa gcactttcca atttgggaaa
     8281 ttattctcct gaaggccaca atcaacgcca acagccatac cccctactat acctgccagc
     8341 aattcatcct caaatggtat attgtttttc ttattattca tgccaatcac ccttgtcaag
     8401 tggttatctt tttatcatat tatataaaac cacgactaac aaaacagcta acaatagctg
     8461 aaatgtcttc ataggaggtc agtgccatgg gaaaagttat cgatttaacc aaaagcgtct
     8521 atgaaatatg caaaaaacat cccgaagtag ttgacatcat gaaagactta gggtttgaga
     8581 acattaccag ccccggcatg ctaaacaccg caggcaggtt tatgactata cctaagggtg
     8641 ctgcgatgaa aggtatcccc atggaaaaaa tcaaagaggc attcgcagca aagggctttg
     8701 atttagaatc atgaccacac atatcttccc tatatcttct tttatttttc cgtatattat
     8761 ttgccttctg tatttaagtg caaatactat atgatttttg caattccatt tagtacgtga
     8821 taatgtttta ttatccaatt ggatttacct cctctgattt aatattgcgg ttggccaaac
     8881 ccactcatcg gtttatattt atcaataaga tattatcttc tgtataaaag tacctttttt
     8941 caaatcatca aaagccttat tcaattcttc cttggtgttc atgacaatag ggccacccca
     9001 aacaataggt tcgtccaaac gtatcgaact aataaacagt acttgagctt ttttatcggt
     9061 agcctttatt tctacttggt cgcctgaagt gagtttcacc gccgtctttt cctttacaag
     9121 ttcacctccg atatatgcgt cccccaaaag ggtaaacacc atgac
//
LOCUS       DAONUO010000036         9037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5902_length_9037_cov_4.492875, whole genome shotgun sequence.
ACCESSION   DAONUO010000036 DAONUO010000000
VERSION     DAONUO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5902_length_9037_cov_4.492875"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PK768_02175"
     CDS             <1..659
                     /locus_tag="PK768_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HQA59807.1"
                     /translation="GAGNPGKYMSKLKQAGIKVIPVVSSVALARRLERVGADALIAEG
                     MECGGHIGELTTMALVPQVVDAVKIPVIAAGGIADARGFLAALALGAKGVQMGTRFVC
                     ARECTAHEKFKEAIITAKDRSTIVTGKTTGHPVRVIKNKLSLKFEELEQKQASVFELE
                     ALGQGKLRLAVVDGDVENGSVMAGQVSGMIKEVKSAENIIQDIIKGAAKLLNSLSSLS
                     "
     gene            675..1610
                     /gene="fabD"
                     /locus_tag="PK768_02180"
     CDS             675..1610
                     /gene="fabD"
                     /locus_tag="PK768_02180"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQA59808.1"
                     /translation="MGKTAFIFSGQGAQYVGMGKDLYDNFHTARRVYEEADEVLKDKI
                     SKLCFEGPDEKLQETTNTQPAILTHSIACFSVLKEEGIVPDVVAGLSLGEYSALVASE
                     SLEFRDAVPLVQKRGKYMQEAVPVGKGTMAAIIGLEREKVLEICQMASTEGVVEAANF
                     NCPGQIVLAGEVRAVIKAMELAKAKGAKRVVMLSVSAPFHCSLLKPAADNLAKELENV
                     TIKDIKIPVISNVNADFVSEGNKVKELLIKQVNSPVLWEDCIKKMINSGVDTFVEVGP
                     GKALSSFVKKIDKTLKVSNVEDTESFYKTIKNLGG"
     gene            1615..2355
                     /gene="fabG"
                     /locus_tag="PK768_02185"
     CDS             1615..2355
                     /gene="fabG"
                     /locus_tag="PK768_02185"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HQA59809.1"
                     /translation="MDLAGKVCLVTGGSRGIGKAICLQLAKEQAKVIVNYSSSENMAA
                     EVVKEIEDLGQQAIKYSANVSDFAQVSKMVDDIIGEFGRIDVLVNNAGVTRDTLILRM
                     KEEDWNKVIDVNLKGVFNCSKACAKYMIKQKKGKIINISSIVGIYGNIGQVNYAASKA
                     GIIGLTKSLAKELGSRGITVNAVAPGFIETDMTASLLEKNSDIISMIALKRLGSPHDV
                     ANLVVFLASKKADYITGQIIAIDGGMTL"
     gene            2404..2658
                     /gene="acpP"
                     /locus_tag="PK768_02190"
     CDS             2404..2658
                     /gene="acpP"
                     /locus_tag="PK768_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQA59810.1"
                     /translation="MRKGGDTVEILDRIKQIIADQLGIDEDQIVPEAAFIDDLGADSL
                     DIVELIMALEEEFDMEIPDEDAEKIKTVQDAIDYIKNHSA"
     gene            2842..4083
                     /gene="fabF"
                     /locus_tag="PK768_02195"
     CDS             2842..4083
                     /gene="fabF"
                     /locus_tag="PK768_02195"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQA59811.1"
                     /translation="MEKKRVVVTGMGVISPVGTGLNKFWDSLVKGKSGIDKITRFDTS
                     DLPSRIAGEVRDFNPEAYIEKKELKRLDRFTQFAISATKMALEDADLDLSTVDKTRIG
                     VILGSGIGGNATWEEQHSILLSKGPKRVSPFFIPMMIVNMASGQVSMAFGLKGPNFTV
                     VTACASGTNAIGEAFRTLQRGDADIIISGGTEAPITMLSLAGFSSMKALSSRNDEPSL
                     ASRPFDRERDGFVMGEGAGILVMESLDSALKRNAKIYGEVVGYGSTADAYHLTQPSPE
                     GEGASRAMEAAISDAGISPSDVDYINAHGTSTPLNDKFETMAIKNTFGEHAYKLAVSS
                     TKSMTGHLLGAAGAVEIVATLLSVYNDEIPPTINYENPDPECDLDYVPNRAVKRTVNI
                     ALSNSMGFGGHNACIIVKKFK"
     gene            4093..4806
                     /gene="rnc"
                     /locus_tag="PK768_02200"
     CDS             4093..4806
                     /gene="rnc"
                     /locus_tag="PK768_02200"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQA59812.1"
                     /translation="MLVDQNRLLQLNALQKKINIQFSDVYTLNHAFIHPSYTNEKGSL
                     YQDNNQRLEFLGDSVLELAVSEYLYRNYDLLTEGQMTKVRAITVCEPSLARVARQLGL
                     GDYLILGRGEENTGGRNKNSILADTFEALIGAIYIDKGYEDAYNFVICNLGAVISKAA
                     YGEGDIDYKTNLQEFLQKSGGDRVFYNVTKETGPDHDKVFYVNVVWKNEILGTGIGKS
                     KKQAEQNAAKAALEKIKGR"
     gene            4910..5170
                     /locus_tag="PK768_02205"
     CDS             4910..5170
                     /locus_tag="PK768_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HQA59813.1"
                     /translation="MEVLKVSAQSKPKSVAGALAAVLREKGVAELQAVGAGAVNQAVK
                     AIAITRGFVAPNGIDLITVPAFAEIDIDGEERTAIKFIVEPR"
     gene            5245..5868
                     /locus_tag="PK768_02210"
     CDS             5245..5868
                     /locus_tag="PK768_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HQA59814.1"
                     /translation="MKNNYLVAINEGWQTLSLKNPEQIAEFMEVTYLKEEGLFLVPHL
                     NENYIVDCNNKTVKSEKDGSIAGTELTILTLHYLTFFKEKENLAHKWVSLKEIPNGGI
                     LFYPAFYADTIKSIIKAFGNNTELFQACAKELGGYSGIFGTTSALFHVFPKIPICVVI
                     WQGDEEIPANATVMFDKSVSRFLHIESIIGVGSYLAKRLIFLARKKT"
     gene            5936..>9037
                     /gene="smc"
                     /locus_tag="PK768_02215"
     CDS             5936..>9037
                     /gene="smc"
                     /locus_tag="PK768_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQA59815.1"
                     /translation="MYLKRIELYGFKSFANRVELEFQPGINAIVGPNGSGKSNIIDAI
                     RWVLGEQSVKILRGYKLEDVIFAGSHMKKPMGMAEVAITLDNSDRLIPLDYSEISFVR
                     RTFRSGESEFFINKTPCRLKDIQEILIDSGVGKDGYSVISQGQIDEILTYRPEERRVI
                     FEEAAGIMKHKIRKREAEKKLEETMDNILRIDDVINELLNQLEPLAEQKEIALKYNEL
                     SLSYKEIDVNLLLYRRDNRKKKLKLILNELQNNKKALTKIHNMIEVLKQKLAEFKSKQ
                     NEVEKTYENAQADLYSVNSQIKDLNKDFQVTTAEKERLEKENNDFCNKINGHEKALEK
                     SKRELKIKTDTLEEKIKTINQLELTLEEREKMLLFLNEEIDSKQKLIESVKGDVIDLL
                     NYASEKRNVISQLNTSKNNLEKRQKQIEKEMEQIQYANSQTLKEISQINEEITRLTSL
                     CDELELNIKELINLENQLAAKLEKNEKQVLQKQGELAVVTSRLKTLKEIDASYEGYQH
                     GVKNLLIALKTKKYLDDGIYGSVADIISVKERYEVAIETALGQGMQNIICESEENARK
                     AIEFLKRNNYGRVTFLPLSTVKPRSLDKSEEKYLSISGCIGTALELISFDNKFFNPLA
                     FLLGRVLVAKSLEDALKIARQSNFAFKIVTIQGELLSPGGSITGGSQRRSSFLLSRKR
                     QINDCEGKITELNDILTDLKEDNQKLREEIEDKRRLIEKSTASLYSAKSDLEVLKKQL
                     QEKHYVLTERQEREKQLDEEKQHIQLESLEIIEQIASLEVDIGDIDIRNTSTQRRVKS
                     LQEEIDKLKEKKDEVSDEITELKVKLASYKQEQIAISQSLQALEENINLYIKEIRECQ
                     EKITKNDEVISLMGNMLIDYKNTINDLMNTYRQQQELLKVTKEQKDALKSNIDKTQDE
                     LNKLEKECHQIETTIHNLHIQETTMNIELNQIDNQLYERYSLNFEEALKLKRDFPSIE
                     ELKNSLVSLKDQMESLGPVNMKAIEDYDNLKARYDFLITQRDDLIKAKANLDSIIEEI
                     TNTM"
BASE COUNT         3220 a         1447 c         2025 g         2345 t
ORIGIN      
        1 cgggtgcagg aaatcctgga aaatatatgt ctaaacttaa gcaagcaggt atcaaagtta
       61 tacccgttgt ttcatcagtt gcattggctc gaagactcga aagagtaggc gcagatgcct
      121 tgatcgccga aggcatggag tgtggcggcc atataggtga attgacaacg atggcattgg
      181 tgccacaagt ggtagatgcc gtgaaaatac ctgtaattgc agcaggaggt atcgcggatg
      241 ctagaggatt tcttgcggca ttggccttgg gagcaaaggg ggttcagatg ggcactcgct
      301 ttgtttgcgc cagagaatgt acggcacacg aaaagtttaa agaagccata atcactgcaa
      361 aagatagaag tacaattgta actggaaaga caacgggcca tccggtgaga gttataaaaa
      421 acaaactgtc tcttaagttt gaagagttgg agcaaaagca agctagcgtt tttgagttgg
      481 aagccctagg tcaaggcaag cttcgacttg ccgttgtcga tggtgatgtg gaaaacggtt
      541 ctgtgatggc tggccaggtt tccggcatga tcaaagaagt aaaaagtgct gaaaacatca
      601 ttcaggatat tatcaaaggt gcagcgaagc tgcttaatag tttatcaagt ttaagctgat
      661 tggaggtgcc aagagtggga aaaacagcat tcattttttc gggtcaaggt gcacaatacg
      721 taggaatggg caaggacctt tacgacaatt ttcatacagc gcgaagggta tacgaagaag
      781 cagatgaggt tttaaaagac aaaatttcca aattgtgttt tgaaggacca gatgaaaaat
      841 tgcaggaaac cacaaatact caaccggcta ttttaactca cagtatagca tgtttctctg
      901 tacttaaaga ggaagggata gttcccgatg ttgtagcagg tttgagtttg ggcgaatact
      961 cggccctcgt ggcatctgaa tcattggagt ttcgtgatgc tgtaccttta gttcaaaagc
     1021 gaggcaagta catgcaggaa gcagttcctg taggcaaagg cactatggca gcaataatag
     1081 gccttgaaag ggaaaaggtt ttagaaatct gccaaatggc ttctaccgaa ggagttgttg
     1141 aagcggccaa cttcaattgc cccggtcaaa tagtgttggc aggagaagtc agagccgtga
     1201 ttaaagccat ggagctagct aaagcaaaag gtgccaagag agttgtgatg ctttcagtca
     1261 gtgcaccctt tcactgcagt ttattaaagc ctgcagcaga caatttggcg aaagaactgg
     1321 aaaatgtaac aattaaagac attaaaattc ccgttatttc aaatgtaaat gcagattttg
     1381 taagtgaagg caataaggtt aaagaactat taataaaaca ggtaaattct cccgtgttat
     1441 gggaagactg tattaaaaag atgataaata gtggtgtaga cacttttgtt gaggttggtc
     1501 caggcaaggc tttaagcagt ttcgtcaaaa aaattgataa aacgctaaaa gtatcaaacg
     1561 tggaagatac ggagtccttt tacaaaacca tcaaaaattt gggaggctga agtgatggat
     1621 cttgctggca aagtgtgcct ggtgacaggc ggttccagag gtatagggaa ggcaatatgt
     1681 ttgcaattag cgaaggagca agcaaaggtc atagtcaact attcatcatc ggaaaacatg
     1741 gctgcagaag tagtaaagga aatagaagat ttaggtcaac aagcgataaa gtatagtgca
     1801 aatgtatctg actttgcaca ggtgagtaag atggttgacg atataatagg tgaatttggg
     1861 agaatcgatg tcttggtaaa taatgctggc gtgacgagag ataccttgat tttgcgaatg
     1921 aaggaggagg attggaataa ggttatagat gttaacttaa aaggagtgtt caactgttca
     1981 aaagcttgtg caaaatacat gataaagcaa aagaagggta aaataatcaa catatcatct
     2041 attgtaggta tatacggtaa tataggccag gtaaattatg ccgcctccaa agctggcata
     2101 ataggactta caaagtcctt agctaaggag ctgggaagtc gtggaattac agtaaatgca
     2161 gttgcccccg gctttattga gacagatatg accgcttcgc tccttgaaaa gaacagcgat
     2221 ataatttcca tgatagcctt aaaaaggcta ggcagccccc atgatgttgc gaatttagta
     2281 gtttttctcg catcaaaaaa agctgactac ataacgggac agataatcgc tattgacggt
     2341 ggtatgactt tgtaatatct ttagaatatg gaataaaact aataaaatta atcatactat
     2401 gcactgagga aggggggtga caccgtggag atactggaca gaataaaaca gattattgcg
     2461 gatcaactag gcatcgatga ggatcagatt gtacctgaag ccgcatttat tgatgattta
     2521 ggagcagatt ctctggacat agtggaactt atcatggccc ttgaagaaga gtttgatatg
     2581 gaaatacctg atgaagatgc ggaaaagatt aagacggtac aggatgccat tgactacata
     2641 aaaaatcact ccgcataatc ataatacgtc ccgtagctca cctacgggac tttgtttttg
     2701 ttgaggcttt tgattttgta atcagggata tcccgttaaa atataatact caattgtgtt
     2761 gaatacaaga ttatcattac tgcacctagg tgactttttg cacttcagca taacaaacta
     2821 tcttctacga ggaggtaaac tatggaaaag aaacgtgttg tagtaaccgg aatgggtgtt
     2881 atttctccgg ttggcaccgg attaaataaa ttctgggact ccttagttaa aggcaaatca
     2941 ggtatagata agattacccg atttgacact agcgatcttc cttccagaat agcaggtgag
     3001 gtaagggatt ttaatccgga agcttacata gaaaaaaaag agctgaaaag attagacagg
     3061 tttactcagt ttgctatatc ggctacaaag atggcccttg aagacgcaga cctggacctt
     3121 tcgactgttg ataaaacaag gataggtgtt attttgggtt caggcatagg aggtaatgca
     3181 acctgggagg agcagcacag catccttttg agcaagggtc caaagagagt aagtcccttt
     3241 tttataccga tgatgatagt caatatggct tcgggacaag tatccatggc ctttggattg
     3301 aaaggtccca attttacagt ggttactgct tgtgcatcag gaacaaatgc tatcggtgaa
     3361 gcatttagga ctttacaaag aggggatgct gatattataa tatcgggagg cacagaagca
     3421 ccaataacaa tgctttccct ggcagggttt tcttccatga aagcattatc tagcagaaat
     3481 gatgagccat cccttgctag ccggcctttc gacagggaaa gggacggctt tgtaatgggt
     3541 gaaggcgctg gaatactagt aatggaatca ttagatagtg cattgaagag aaatgcaaaa
     3601 atttacggtg aagttgtagg ttatggctca acagccgatg cctatcattt gacacagcca
     3661 tcaccggaag gggaaggtgc ctctcgtgca atggaagctg caatatccga tgctggcatt
     3721 tctccaagtg atgtggatta tataaatgca catgggacct ccacgccctt aaatgacaaa
     3781 ttcgaaacta tggcgataaa aaataccttt ggtgagcatg catataagct tgcagtaagt
     3841 tccacaaaat cgatgaccgg ccatttgcta ggtgccgcag gcgcagtgga aattgtcgca
     3901 acacttcttt cagtttataa tgatgagatt ccacctacaa tcaactatga aaaccccgat
     3961 cccgaatgtg atttggacta cgtgcccaat agagctgtaa aacgaactgt aaacatagcc
     4021 ttatcaaatt cgatgggttt tggcggccat aacgcatgca ttattgtaaa aaaatttaaa
     4081 tgaggaataa atatgttggt agaccaaaat aggcttttac aactcaacgc tttacaaaag
     4141 aagataaata ttcaatttag tgatgtttat accttaaatc atgcctttat acacccatct
     4201 tatactaatg agaaaggaag tctgtaccag gataataatc aaagattaga gtttttggga
     4261 gattccgtac ttgaattagc tgtaagtgaa tacctttata gaaattatga tttactaact
     4321 gaagggcaga tgacaaaagt tcgcgctatt actgtttgcg aaccaagtct agctagggtt
     4381 gcaaggcaat tgggcttggg tgactatctt attttgggaa gaggagaaga aaacacagga
     4441 gggcggaata aaaattcaat ccttgcagac acttttgaag cattaattgg ggcaatctat
     4501 atagacaaag gttatgagga tgcttataat tttgtaattt gcaatttagg agctgtgata
     4561 tcaaaagctg cgtatggcga gggagacatt gattataaga caaatcttca agaattcctg
     4621 caaaagtcag gtggcgacag agtcttttac aatgtgacaa aagaaacagg cccggatcat
     4681 gacaaggtgt tttacgtaaa tgtagtgtgg aaaaacgaga ttttgggcac cgggataggc
     4741 aagagtaaaa aacaagcgga gcaaaatgca gcaaaggctg cccttgaaaa aataaagggt
     4801 cgctagaaaa atataatatt gcaaaaagaa ggaattttgt aacccttata gaatatatat
     4861 tgcataatat aaaaaaataa taagcatgaa taagggggat atttcgaaaa tggaagtact
     4921 aaaagtgtca gctcaatcta agcctaaatc tgttgcaggg gctttagcag ctgtactaag
     4981 agaaaagggt gtggcagagc ttcaagccgt gggagccgga gctgtaaatc aggcagtgaa
     5041 ggccatagca attaccaggg gctttgttgc accaaacggt atcgatttaa taacagttcc
     5101 tgcctttgcg gaaattgaca ttgacggcga agaaagaaca gcgataaaat ttattgttga
     5161 accccgataa atatcttatg attatacctg tctgttaaga caggtatttt tatgtagaag
     5221 cttttgcgtt atgaggggat tgaaatgaag aataattatc ttgttgcaat taacgaaggt
     5281 tggcaaactc tttctttaaa aaacccggaa caaatagcgg agtttatgga agttacatac
     5341 ttaaaggaag aaggcctttt tcttgtaccg catcttaacg agaactatat agtggattgc
     5401 aacaataaga ctgtaaagag tgaaaaagat ggttcaattg ccggtacaga actgacaatc
     5461 cttacacttc actacttgac attctttaaa gaaaaggaaa atttagcaca caagtgggtc
     5521 agtctcaaag aaatcccaaa tgggggtatt ttattttacc cggcctttta cgctgatacg
     5581 attaaaagca ttattaaagc attcggaaac aatacagagc tttttcaagc atgtgccaaa
     5641 gagctaggag gctattccgg tatttttggc acaacatctg cactgtttca tgtgtttccc
     5701 aaaatcccaa tttgtgtggt tatttggcag ggagatgaag aaataccggc gaatgcaacc
     5761 gttatgtttg ataaatcggt tagccgtttt ttgcatatag agagtatcat cggtgtcgga
     5821 agttaccttg caaaaagatt aattttcctt gcccgtaaaa aaacttgatt tatgctcggt
     5881 gagttctttt tttgtgtggc ttgtgttaga attgataagg tagggaggga tgccgttgta
     5941 tctgaaaaga atcgagcttt acggctttaa atcctttgcc aatagagtag aactcgaatt
     6001 tcaaccagga attaatgcaa tagtaggtcc aaatggcagc gggaagagca acataatcga
     6061 tgccattcga tgggtattgg gtgaacaaag tgtaaaaatt ctaagaggat acaaacttga
     6121 agatgtgata tttgcaggca gtcatatgaa aaagcctatg ggtatggcgg aggttgccat
     6181 tacccttgac aattccgacc gcttaatacc cttggattat tctgaaataa gttttgtacg
     6241 tcgaaccttt agatcgggtg aaagtgaatt tttcataaat aaaactccct gcaggctaaa
     6301 agatattcag gaaatcctca ttgattccgg agttgggaaa gatggttatt cagttataag
     6361 ccagggtcag atagatgaaa ttttgactta taggccggaa gaaaggcggg taatttttga
     6421 agaagctgca gggataatga aacataaaat tagaaaacgt gaagctgaaa aaaaacttga
     6481 agaaaccatg gacaacattt tgcgaataga tgatgtaata aatgaactat taaatcagtt
     6541 ggagccttta gccgaacaaa aagaaattgc attaaagtat aatgagctta gcttatcata
     6601 taaagaaata gatgtaaatt tacttttgta caggcgggat aacaggaaga aaaagttaaa
     6661 gctcatattg aatgagctcc agaacaataa aaaggcattg actaaaattc ataacatgat
     6721 agaagtattg aaacaaaaac tagcagaatt taaatcaaaa caaaatgaag ttgaaaaaac
     6781 atatgaaaat gcccaggctg atttatacag tgtgaatagc cagataaaag atttaaacaa
     6841 agactttcaa gtaacgacag ctgaaaaaga aagacttgaa aaggaaaata atgacttttg
     6901 caacaagatc aatggacacg aaaaagcctt ggaaaaatcc aaaagagagc taaaaataaa
     6961 aacggatact ctagaagaaa aaataaaaac cataaaccag cttgaattga cattagaaga
     7021 aagagagaaa atgctccttt tcctcaatga ggaaatagat tcaaaacaaa agctcattga
     7081 aagcgtaaaa ggtgatgtaa ttgatttatt aaattatgct tcggaaaaaa gaaatgtcat
     7141 ctctcaactc aatacgtcga aaaacaactt ggaaaaacgt caaaagcaga ttgaaaagga
     7201 aatggagcaa atacagtatg ctaacagcca aacccttaaa gaaatctctc aaataaacga
     7261 agagattact cgcctaacat ccctgtgtga tgaactggaa ctgaatatca aggagctaat
     7321 taatcttgaa aatcaactgg ctgcaaaact tgaaaaaaat gaaaagcaag tattacaaaa
     7381 acaaggagaa cttgctgttg taacgtcccg cttgaagaca ttgaaggaaa ttgacgcaag
     7441 ttatgagggt tatcaacacg gtgtaaagaa cttattaatt gcactaaaaa ctaaaaaata
     7501 tttagatgat ggcatctacg gctccgttgc cgatataatt tctgtaaaag aaagatacga
     7561 agttgccatc gaaacagctt tgggtcaggg catgcagaat ataatatgtg aaagtgaaga
     7621 aaatgctagg aaggcaatag aatttcttaa aagaaacaat tacggtcgag ttacattttt
     7681 accactttct acagtaaagc ccaggtcttt agataagagc gaggagaagt atttgtcaat
     7741 tagcgggtgc ataggaacag cgctggaact cattagcttt gataacaaat tttttaatcc
     7801 tttagcattc cttttgggca gggttttagt agcaaagtcc ttagaagatg ctttaaagat
     7861 agcaagacag agcaactttg cttttaaaat tgttacaatc caaggtgagc tactgagccc
     7921 aggtggctct ataacaggtg gaagccaaag acgatcgagc tttctattat ccagaaagag
     7981 acaaataaat gactgtgaag gcaaaattac agaacttaat gatatcttaa ccgaccttaa
     8041 agaggataat caaaagctaa gagaggagat tgaagataag cgcagactta tagaaaaaag
     8101 caccgctagt ttgtattctg caaaatcaga ccttgaagtt ttaaaaaagc agctacaaga
     8161 aaaacattat gttttaaccg aaagacagga gagagaaaaa cagttggatg aggaaaaaca
     8221 acacatccag ttagaatctc tagaaataat agagcaaatt gcctcccttg aagtagatat
     8281 tggtgatatt gatatacgga acacttccac tcaacgcaga gtaaaaagtc tacaagaaga
     8341 gatagataag ttgaaagaga aaaaggatga agtatcagat gaaataaccg agcttaaagt
     8401 taaactggca tcttataaac aggaacaaat tgctataagt cagagcttac aagccttaga
     8461 agaaaacata aatctgtata taaaagaaat cagagaatgt caagaaaaaa ttacaaaaaa
     8521 tgacgaagta atatcattga tgggtaatat gctaatagat tataaaaata ccatcaatga
     8581 tctgatgaac acttatagac agcagcagga gcttctcaag gtcaccaagg aacaaaaaga
     8641 tgcattaaaa tcaaatatag ataaaactca ggatgagctg aacaagctcg aaaaagaatg
     8701 ccatcaaata gaaacaacaa ttcacaactt acatattcaa gaaacaacaa tgaatataga
     8761 attaaatcaa attgacaatc aattatatga acgttattct cttaacttcg aggaagcact
     8821 aaagttaaaa agagactttc ctagtattga agaattaaaa aacagtttag tatctttaaa
     8881 agatcaaatg gaatcccttg gccccgtaaa tatgaaggcc atagaagact atgacaatct
     8941 aaaagctagg tatgattttt tgatcacaca gagggatgat ttaataaaag ccaaggcaaa
     9001 tcttgacagc ataatcgaag agatcactaa taccatg
//
LOCUS       DAONUO010000037         9032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5905_length_9032_cov_3.645204, whole genome shotgun sequence.
ACCESSION   DAONUO010000037 DAONUO010000000
VERSION     DAONUO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5905_length_9032_cov_3.645204"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK768_02220"
     CDS             <1..332
                     /locus_tag="PK768_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HQA59816.1"
                     /translation="NIFIPSASAKWGILSSIFVPMLMILGYNPAFTQLAYRIGDSITN
                     PITPLMPYFAILLGYAQQYDKNIGMGTLISLLIPYSMIMGVFWIILFIIWFLLGLPVG
                     PGAPIFL"
     gene            361..1545
                     /locus_tag="PK768_02225"
     CDS             361..1545
                     /locus_tag="PK768_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQA59817.1"
                     /translation="MIDVLAYIRQEAEKLYPEAVKMRRDFHRHPELGFKEVRTAKVVA
                     DYLRALNLEVTEGVGKTGVVAVLSGDQAGKTVALRADMDALPIQEETGLDFASEIPGV
                     MHACGHDGHTAMLLQVAKLLSSMKSRLKGRVKFIFQAAEELSHGGATLMCQEGAVDDV
                     DAIFGLHIWPTLETGHIGVLSGPMMASADYGYVTVKGKTGHGSQPHKAVDACVVAAQA
                     ILGIQTVVSRQIDALDPAVISIGSISGGTAANVIADEVKFAFTVRALSKENREKVPVL
                     VERTIKGITESAGATYEFNYDYCLPPVINDSKAVALVEEVGKQLLGEDKVIRLSKPVM
                     GSEDFSFFQEKVPGAYFFLGGKNEEKGIVPLAHNSKYNFDEDAMLTGISMMAAIAHKF
                     LS"
     gene            complement(1647..2444)
                     /gene="murI"
                     /locus_tag="PK768_02230"
     CDS             complement(1647..2444)
                     /gene="murI"
                     /locus_tag="PK768_02230"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQA59818.1"
                     /translation="MSNIPIGVFDSGIGGLTVAGEIMKLLPGENIVYFGDTARVPYGS
                     KSPEAITQFAFEGLKFLLSQDVKLIVIACNTVSATCLEELAASTRVPVLGVIEPGARA
                     AVNATRNHRVGVIGTERTTKSRAYERAINALNESISIYSKACPLFVPLVEEGWLENEA
                     ALITAKIYLEPLKGQGIDTLVLACTHYPLLKPAIGQIMGRGVRLVDSAEETAKEVWQI
                     LNGGNLLRKANAPASYRYFVSDDPEKFVRVGEKFLKRSIEPISVIEP"
     gene            complement(2605..3873)
                     /locus_tag="PK768_02235"
     CDS             complement(2605..3873)
                     /locus_tag="PK768_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQA59819.1"
                     /translation="MSLPRVAFGSRQLKKQRPLIKGTDVRRLQQALKHLGFFRARVDG
                     VFGYRTSEAVREFQSFFKLKQNGKVSDDEFNILTELVQAGINKWYTPCHDFAHTGYLP
                     APITAQLTLSKTWNISDVLWLNSTSDRLIVTTKEQVLAISLISGKVLWRTAKIFPVAA
                     PVISEGQLLIPAQNLEILDLFSGKRLQSISQDNFTTAVAARGGKIYASSWGSLHAFDR
                     KGKALWKYRTSGAFCTSPTLGYDLIYFASYDRNIYCLDDEGTLYWKTKVSDMIKLPLA
                     IWDGKIFAVSQDARIYAINPLLGSLIWQKKFTDDELMMPAFHPDFMLLANYKGEVVAI
                     SFQSGQTKWAADLPAAPTTSPVVLKKTCFIGTEGGLVAYDIESLKCRRFLEGETIRAI
                     APAALSLFVATDKKLVKLVPNQEPHPTRKQ"
     gene            4170..5453
                     /locus_tag="PK768_02240"
     CDS             4170..5453
                     /locus_tag="PK768_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HQA59820.1"
                     /translation="MITRREFLKLCLSSAALSLSGILLPKVAEALDERGEKVTVIWLE
                     MMTCTGDFLSFANTLNPDVEQLIKDTINLEFQNTMMAAEGELALQHLYDVASREEGRY
                     ILVAEGTVPTRDNGFYGITGYYEDGTPLKDIDAVKFLGKRAKHVIAIGTCASFGGPYA
                     ASPNPSGSVPVHKVLKGIQVINVPGCPAHPDWFVGTLSHVILYGVPSLDAFGRPRMFY
                     RHTIHDFCPRRQHFENGIFAKEPGDEGCLFQIGCKGPVTYSDCPTRKWNSMHLNWPVG
                     ANTPCIGCVSPEFPDRMLPFFAHLQDVNLPNINVNASLIGKAAIGTAAAAIGTHAIVS
                     AAKGRIFRHLAESSETKDVGKELEKVVEEKLSGDKISDEDMQGVEKSPSGSENKSVAK
                     PENTAKHCEEISRELIPSRPKIKRPKKLVTRKLRR"
     gene            5458..6861
                     /locus_tag="PK768_02245"
     CDS             5458..6861
                     /locus_tag="PK768_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQA59821.1"
                     /translation="MPTKIISPITRLNGFWRIDVNIENGQVVDAYSSGIFFRGIEKIL
                     HGRDPRDAVYLTQRICGICSTAHGLASSLALEDAFKIKPPRNGIIIRNLLFGADLLQN
                     HIRHFYFLTVPDFVRGPDRPPFVPRYEVDYRLSRTESDKIYENYMESIHISRAVHDMV
                     TIFGGKIPHNHGILAGGATVPPTADNVRMFMSLLQKVRSFVQNKMMADLEKIATAYED
                     CYKIGIGTGNLMAFGMFPHPDKKDDFYFKPGIIIGKKREKLDAMAITEHIKYSWYRAS
                     PPLHPEEGETQPDTGKEDAYSWVKAPRYKGHPVEGGPLARLILSGDYKGGHSAMDRIA
                     ARVMEANIVSEMMEEWCRQLEIGKPVFTPYDMPANAEGIGMVEAMRGGLGHWMRMRAG
                     RITHYQIITPSAWNCSPRDDEGRRGPVEEALIGTPIEDFDQPIEVGRVARSFDPCLAC
                     AVHMIDMKGREKYVEIL"
     gene            6845..7525
                     /locus_tag="PK768_02250"
     CDS             6845..7525
                     /locus_tag="PK768_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQA59822.1"
                     /translation="MLKSFEESKEAQDIAVKKRNIVTISYVNILLYPLPIRIFHWVLV
                     ASLYLTIISGLMISCAYALVPMKSIRFIHISAGFVAFGIVLYRLGYAFMSGDYRNFSI
                     KLKDFKTFPDLAKYYLFLRENPPALRTKYNIGQKITMLSWLMGILYQTFTGIMLLNSS
                     FVSKTSALSLLSKLILPQYLRTTKYFISIYFVITIMLHIYLAHTEDIAKSQSMLTGWV
                     RVKRKNNN"
     gene            complement(7638..7829)
                     /locus_tag="PK768_02255"
     CDS             complement(7638..7829)
                     /locus_tag="PK768_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HQA59823.1"
                     /translation="MARRRSVMSEDLKYELAKELGVADTVAKKGWGEVSSRNCGNLVR
                     LAIEKAEKALAEQNGKTQS"
     gene            complement(8026..>9032)
                     /locus_tag="PK768_02260"
     CDS             complement(8026..>9032)
                     /locus_tag="PK768_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HQA59824.1"
                     /translation="VFVINPTYYGVASDLRKIVEIAHSYGKPVIVDEAHGAHLGFHPD
                     LPLSAMEAGADMSAVSVHKLLGSMTQSSILLVKGDLIDDRKVKAAMNLTQTTSPSYPL
                     LASIDVARKQAALKGRELLQRTIELCERVREELNGIKGIYVMGRDVEGTEGCFALDPT
                     KLAINLREIGLSGFETEKLLKYEYGIQMELSDLYNVLALGTIADNEASMRTLVNAIKD
                     IVQKYGSKNLIKITTELPDNQELVVSPRFAFYSEKRVVPLEEAEGEISAEMLMAYPPG
                     IPIICPGERISKEVIEYAKTLKSEGCQMEGTFDPDARTIMVLADIESVKYYERIKEVV
                     "
BASE COUNT         2398 a         2147 c         2101 g         2386 t
ORIGIN      
        1 taaatatctt catccccagc gcatctgcaa aatggggtat cctgtcttcg atctttgtcc
       61 ccatgctcat gatcctgggt tataacccag cttttaccca attggcatat agaataggtg
      121 actccatcac aaatcccata acaccgctga tgccatactt tgccatcctg ctaggatatg
      181 cccagcagta tgacaaaaac atcggaatgg gaacattaat ttctctgctg attccttatt
      241 ccatgataat gggagtgttc tggataatct tgtttataat ttggttcttg ctgggcttac
      301 cggtaggtcc cggtgcaccc atatttttgt aaaatttccc aacaactttg aggtgattaa
      361 atgattgatg tgttagccta tataaggcaa gaagctgaaa agctgtaccc ggaagccgtg
      421 aaaatgcgcc gcgatttcca caggcacccg gaactgggct ttaaagaagt tcgcacggca
      481 aaagtagtgg cagattattt gcgggcttta aatcttgagg ttaccgaagg cgtgggcaaa
      541 accggcgttg tggcagttct aagcggggac caggctggca agacggtggc cctgagggct
      601 gatatggatg cccttccaat tcaggaagag acgggccttg attttgcatc ggaaatcccc
      661 ggcgtaatgc atgcctgcgg ccatgatggc catacggcca tgctccttca ggttgcaaag
      721 ctcctatcat cgatgaaatc caggctgaaa ggccgggtca aattcatctt ccaggcggcg
      781 gaagaattgt cccacggggg cgccaccttg atgtgtcagg aaggagcagt cgatgacgta
      841 gatgcaattt ttgggctgca catatggccg acccttgaaa caggccatat tggcgtattg
      901 agcggcccca tgatggccag tgcagactac ggctatgtaa ccgtaaaggg aaaaacaggt
      961 cacggctcac agcctcacaa ggctgtagat gcctgcgtgg ttgcggcaca agccatactt
     1021 ggaatacaaa ctgtagtcag ccgtcaaata gatgctttgg acccggcagt aatcagcata
     1081 gggtctattt cagggggcac tgcggcaaat gtcattgccg atgaggttaa attcgccttt
     1141 accgtgaggg ccttgagcaa ggagaaccgc gaaaaagttc cggtacttgt agaaaggaca
     1201 ataaaaggaa taaccgaaag tgccggcgca acatacgaat ttaattacga ttactgcctg
     1261 ccgccggtaa tcaatgacag caaagctgta gccctcgttg aagaggtagg aaaacagctt
     1321 ctcggggaag ataaagtaat aaggctcagc aagcccgtaa tgggttccga agacttttcc
     1381 tttttccagg aaaaagtccc aggtgcctac ttcttcctgg gtggcaaaaa cgaggagaag
     1441 ggaattgtcc cactggcaca caacagcaag tacaactttg acgaagatgc catgctcacg
     1501 ggcatatcaa tgatggcggc tattgcacat aagtttttat cttaaaaata tcggcagctg
     1561 cataaaaaac atcttgcttt tgcaggaaat tactttcagc taacaacaat aatgaccacc
     1621 tgaaaagggg tggtcattat tttatgttat ggttcaatta cgcttatggg ctcaatggat
     1681 cttttaagga acttttcgcc cacacggaca aatttttccg gatcgtcact gacaaaatac
     1741 cgataacttg ccggtgcatt ggcttttcga agtaggttgc ccccatttaa tatctgccaa
     1801 acttccttgg ccgtctcttc tgccgagtcc accagccgaa ctcctcttcc catgatttgc
     1861 cctattgccg gctttaaaag tggatagtgg gtacaggcta gtaccagggt atctattccc
     1921 tgccccttta ggggttccag gtagattttg gcggtgatta aggcagcttc attttccagc
     1981 cagccctctt ctaccagcgg aacaaacaag gggcaggcct ttgaatatat gctgatgctt
     2041 tcgtttaagg cattaatggc cctttcgtag gcacggcttt ttgtggtgcg ctcggtgccg
     2101 attactccca ccctgtgatt cctggtagca tttaccgcag ccctggcccc cggctctata
     2161 actccaagga ccggcaccct tgttgaggcg gcaagctctt caagacaggt ggcactgacg
     2221 gtgttgcagg ctatgacgat tagtttaaca tcctggctca gcaagaattt taggccttca
     2281 aaggcaaatt gcgtaatcgc ttccggtgat ttggaaccat agggcacccg tgccgtgtct
     2341 ccaaaataga ctatattttc tccgggtaac agtttcatga tttcgccggc tacagtcagg
     2401 cctccaatac ccgaatcaaa gactccgatg ggtatattgc tcatttggat tgttcagctc
     2461 cgtcctgttt tgtgaatttg tgtattttat acacgatgtt attgtattag ctatggtcaa
     2521 taataccaca cttaaacagt agggacaata aaatgaggat gggattcttt tacaaagcgc
     2581 aagtttcccg ggacccaact gcaatcattg cttgcgtgtt gggtggggtt cttggttcgg
     2641 cacaagcttc accaactttt tatcagttgc cacaaacagg ctcaaagctg ccggcgcaat
     2701 cgcccttatc gtttcacctt ccaggaaccg tctacatttt aaactttcaa tatcgtatgc
     2761 cacgagcccc ccttcggtcc ctataaaaca cgttttcttc aatactacgg gcgatgttgt
     2821 gggagcagca ggcaagtctg ccgcccattt tgtctggcca ctttgaaagc tgatagcgac
     2881 cacttctccc ttgtaatttg ccaggagcat aaaatccgga tggaaggccg gcatcataag
     2941 ctcatcatcg gtaaactttt tttgccatat gaggcttccc aacaggggat ttatggcata
     3001 aatccgagca tcctgcgata ctgcaaaaat cttgccgtcc catatggcca ggggaagctt
     3061 tatcatatcg gagacttttg tcttccagta gagggtccct tcgtcatcga gacagtagat
     3121 gtttctgtcg tacgaggcaa agtaaatcaa atcatatccc aaggtcggtg aagtgcaaaa
     3181 ggcgccagag gtcctgtact tccacagtgc cttgcccttt cggtcgaagg catgaaggct
     3241 tccccatgaa gaggcataaa ttttcccacc ccttgcggca acggctgtag taaaattatc
     3301 ttggcttatg ctttgtagcc ttttgccgga aaataaatcg agtatttcaa ggttttgtgc
     3361 tggtatgagc agttgccctt cggatatgac aggggctgcc acgggaaaaa tcttagcagt
     3421 cctccaaagc actttccccg atattaagct tatggcgagg acctgctctt ttgttgtcac
     3481 aatcaacctg tctgaggtgg agtttagcca aagaacatcg gaaatattcc aggtcttgct
     3541 aagtgtaagt tgtgcagtaa tcggagccgg caagtaccca gtatgggcaa agtcatgaca
     3601 gggagtgtac cacttgttta ttcctgcctg caccagttct gttaatatat taaactcatc
     3661 gtccgagact ttaccatttt gttttagctt gaaaaagctc tgaaattccc tgaccgcctc
     3721 ggaggttcta tatccaaata ccccgtcaac tcgggcacga aaaaatccca aatgttttaa
     3781 ggcctgctgc agccgacgca catcggtccc cttaatcagg ggcctttgtt tttttagctg
     3841 acgactgcca aaagctactc taggcaagct caaggcaaca ccacctagcc ttataataag
     3901 ctttgcgata taaatattat attcagtgca caagcagtcg gttacagctc tttgccccga
     3961 ataaatcgtc aacagaaggt aaattctatt aacaagccca taaaaatgaa cttttgaatt
     4021 ttttgggacg ggtacaaaat attcatgaac tttaaacttt tggggacggg tacaaaatat
     4081 tcaaagcgga gccggttccg cccaacaaca ttagcagaag ccaaatatgg caagcgaaac
     4141 gtccccctgc catgtttaag gagagttata tgataacgcg gcgggaattt ttaaaactgt
     4201 gcctctcttc agcagctctc agcctgtcgg ggattttgct gccgaaagtt gctgaagctt
     4261 tagatgaacg gggagaaaaa gtaacggtca tatggcttga aatgatgacc tgcaccgggg
     4321 actttctctc ctttgccaac actttaaacc ctgatgtgga gcagctgata aaagatacga
     4381 taaatcttga atttcaaaat accatgatgg ccgccgaagg tgagcttgcc ttacagcacc
     4441 tgtacgatgt ggcaagccgc gaagagggaa ggtacatact ggtggcggaa ggcaccgttc
     4501 ccacaaggga caatggcttt tacggaatta ccggttacta cgaagacggc actcctctta
     4561 aagacatcga tgcagtcaaa ttcttgggca agcgggcaaa gcatgtcata gctataggta
     4621 cctgtgcatc cttcggaggc ccttatgcgg caagtcccaa tccgtcagga tctgtcccgg
     4681 tgcacaaggt attaaaaggg atccaggtca taaacgtgcc tggatgccct gcacatccgg
     4741 actggttcgt gggaacttta tcccatgtta ttttatacgg cgttcccagt ctcgatgcct
     4801 ttggacggcc ccgtatgttt taccggcata ccatacacga tttttgtcca aggcgtcagc
     4861 actttgaaaa cggcattttt gcaaaagaac cgggagatga gggatgccta tttcaaatag
     4921 gctgcaaagg tcccgttaca tactccgact gccctacccg gaagtggaac agcatgcact
     4981 taaattggcc ggtaggtgcc aataccccct gcataggctg cgttagcccc gaattcccag
     5041 atagaatgtt accttttttt gcccatctgc aggatgtgaa tctgcctaat ataaatgtaa
     5101 atgcttcatt aatcgggaaa gctgccatag gcactgccgc agcagccatc ggaactcatg
     5161 ccattgtaag tgccgctaag ggccgcatat tcaggcacct tgcagaatcc agcgaaacca
     5221 aagatgttgg caaagagctg gagaaagttg tggaggagaa gttaagtggg gataagattt
     5281 cggatgaaga catgcaaggc gtggaaaaat ccccgtcagg gtctgaaaat aaatcggttg
     5341 caaagccgga aaacaccgca aaacactgtg aagaaatttc ccgggagctg atcccctctc
     5401 ggccaaaaat aaaaaggccg aaaaaattgg ttaccaggaa gttgagaagg tgattgtttg
     5461 cctactaaga tcatcagtcc aataaccagg ctgaacggtt tttggcgcat cgacgtaaat
     5521 atcgaaaatg gccaggtggt agatgcatat tcaagcggta tctttttcag ggggatagaa
     5581 aagattctac acggcaggga ccccagggat gccgtatatt taacccagcg catatgcggc
     5641 atatgctcta ccgcccacgg ccttgccagc agcctcgccc tggaggacgc ttttaagatt
     5701 aagcccccaa gaaacggcat catcatcagg aatctgctct ttggagcaga ccttttgcaa
     5761 aaccacattc ggcactttta ctttctgacc gtaccggatt ttgtcagggg acccgacagg
     5821 ccaccctttg tcccccgata tgaagtagac tatcgccttt ctagaaccga aagcgataaa
     5881 atttacgaaa actacatgga aagtatacac atatcccggg cagtccatga catggtcacc
     5941 atattcggtg gcaaaatccc ccacaaccac ggcattttgg cgggtggcgc cacggtccca
     6001 cccactgctg acaatgtgcg catgtttatg tcgctacttc aaaaagtgcg gagttttgtt
     6061 caaaataaga tgatggctga tttggaaaaa atagcaacag cttatgaaga ttgttacaag
     6121 ataggcatcg gcaccggcaa tttgatggcc ttcggcatgt ttccccatcc cgacaaaaaa
     6181 gatgattttt actttaagcc cggcataatc atcggcaaaa aacgggaaaa gctggatgcc
     6241 atggccatca ccgagcatat aaagtattcc tggtacagag cttcccctcc cctgcatccg
     6301 gaggaagggg aaacccagcc cgataccggc aaagaggacg catattcctg ggtaaaggcc
     6361 cccaggtaca aaggccatcc ggtggagggc gggccactgg ccaggctcat cttaagcggt
     6421 gattacaagg gcggccacag cgccatggac cggattgccg ctcgagtaat ggaggcgaat
     6481 atcgtatcag agatgatgga agaatggtgc cggcagcttg agataggaaa gcctgtattt
     6541 acaccgtacg atatgccggc aaatgcggag ggaatcggga tggtggaggc catgagaggg
     6601 ggccttggcc actggatgag gatgagagcc ggcaggataa cacattatca aattatcaca
     6661 ccctctgcct ggaactgttc gccgcgggat gacgaaggca ggcgcgggcc cgtcgaggag
     6721 gcattaatag gcacgccgat agaagatttt gaccaaccca tcgaggtggg gagagttgcc
     6781 cgaagctttg acccctgcct tgcctgtgca gtacatatga ttgacatgaa agggcgggaa
     6841 aaatatgttg aaatcctttg aggaatcaaa ggaagctcaa gacatagctg taaaaaaaag
     6901 gaatattgtc acgatctctt atgtcaatat actcttgtat ccactcccca taaggatctt
     6961 tcactgggta ctagtcgcaa gtctttacct taccattatc agcggtttga tgataagttg
     7021 tgcatatgcc ctagttccaa tgaaatcgat aagattcatc cacatatctg ctggctttgt
     7081 ggcctttggc attgtgctgt atcgcctggg gtacgccttt atgtccggag attacaggaa
     7141 tttttccata aaactaaaag attttaagac atttcccgac cttgcaaaat actatctttt
     7201 tttgagggaa aaccctccag ctttaaggac taagtacaac atcggacaga aaataactat
     7261 gctgtcctgg ctaatgggca tactgtatca aacttttacc ggcattatgc tgctaaactc
     7321 atcctttgta tccaaaactt ctgcattatc gcttttgtca aagttgattc tgccccagta
     7381 ccttagaacg accaagtact ttatttcaat ttatttcgtc atcaccatta tgcttcacat
     7441 atacctggcc cataccgagg atattgctaa gagccagtct atgcttaccg gttgggtaag
     7501 ggtaaaaagg aaaaacaaca attaaaaaaa tacaacaatg ctatcagaga tggatgccgt
     7561 ataactgaac tttgccgttt cactaaaaaa ccggagctag aacttcgccc cggtttttcg
     7621 tgcgctttca ggagtgctta gctctgggtt ttgccatttt gttcggcaag ggccttttcc
     7681 gctttttcaa tggcaagtct caccagatta ccgcagtttc tggatgaaac ttcaccccag
     7741 cctttcttcg ccaccgtatc tgccacacca agttccttag ccaactcgta cttaaggtcc
     7801 tcagacatga cactacgacg acgagccatg agcccacctc ctttgttttt cacgcactat
     7861 tattatcttg tgttcaataa aattttttat gatggtaatt tctaaaccgc tttttagcta
     7921 aatttttttt aaaaaataaa agacatgtaa ctgtatatta caaagaatca atgtaaaaaa
     7981 taaggtgtct agaccacgaa aaacgtagtt ttgacacctc tttttttata ccacttcttt
     8041 gattctctca taatatttga ccgactctat atccgccagc accatgattg tccgcgcatc
     8101 cgggtcgaag gttccctcca tctggcatcc ttctgatttt agtgttttgg catattctat
     8161 gacttctttg cttatgcgtt caccgggaca gatgatgggt atccccggcg gatatgccat
     8221 aagcatttcg gcgctgattt caccttccgc ctcttccagg ggaactacgc gcttttcgct
     8281 gtaaaaggca aatcttggcg aaacgactaa ctcctggttg tcaggaagtt cagtggttat
     8341 ctttatcagg tttttactgc cgtatttttg gactatgtct tttattgcgt tcaccagggt
     8401 cctcatactc gcctcattat cggcaatggt gcccaatgcc agcacattat atagatcgga
     8461 aagttccatt tggataccgt attcatattt taacagcttt tcggtttcaa aaccggacag
     8521 tcctatttct cttaagttaa tggcaagttt tgtgggatct aaagcaaagc aaccttcagt
     8581 gccttcaaca tccctgccca taacgtaaat gccctttatg ccatttagct cttctcgcac
     8641 tctctcacaa agctcaatgg tgcgctgcag aagctctctg ccctttaagg ctgcctgctt
     8701 tctggccaca tcgatagagg ccagcaaggg atatgaaggg ctggtggttt gggttaagtt
     8761 catagctgct tttacttttc tgtcatctat gagatcccct tttacgagga gtatcgagct
     8821 ttgcgtcatg gagcccagca gcttgtgaac gctgacggcg ctcatgtcgg caccggcttc
     8881 catggccgat agcggtaaat ccggatgaaa tcccaaatgt gcaccatggg cctcatctac
     8941 aattacgggt ttgccgtaag agtgagctat ttccacaatc ttgcggaggt ctgaagccac
     9001 tccgtagtat gtggggttaa ttacaaaaac cg
//
LOCUS       DAONUO010000038         9027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5909_length_9027_cov_4.368480, whole genome shotgun sequence.
ACCESSION   DAONUO010000038 DAONUO010000000
VERSION     DAONUO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5909_length_9027_cov_4.368480"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PK768_02265"
     CDS             complement(<1..982)
                     /locus_tag="PK768_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59825.1"
                     /translation="MKSINGNRLVNRIKELGQIGRDAENRLTRAAEADMLRVRANMIL
                     PAGTSITWSVTADGANWITRWRARGIETGTVVEVSDDNGYSWRSIGDVSVASVTSDTK
                     ELWAEVIPGNAVMWRAELATTNKDVTPVITGIAGTAVKWEVNNKPKPPIVHVPEEGWY
                     YTTTPTFYWDFVDEDEGDYQTAFQLEVRRQSDDGLVFDSRKINSSETSFTLPTSNLPH
                     IEGPLWQSGTYAFYVRVKVWDMEDVESEWSMPGYFKVLAYERPRIAEIVSAPPEQEIP
                     EKGDDSSHIMILSGMRAADLPKVKAGSKVTVVLDSVGPILNDAASIAIFPY"
     gene            complement(979..2217)
                     /gene="dpaL"
                     /locus_tag="PK768_02270"
     CDS             complement(979..2217)
                     /gene="dpaL"
                     /locus_tag="PK768_02270"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HQA59826.1"
                     /translation="MSRNIELIKANLIDNRKEKPKTSVDFISEEVVKKVNDFHKGFSE
                     YSVTPLHKLDALAKHFGLKNIFVKDESYRFGLNAFKVLGGAYAIGKYLANKLGMDISE
                     VSFEYLRSKEVKEKIGEITFVTATDGNHGRGVAWAANQLGQKSVVYMPRGSSLTRLNN
                     IRKEGADASIIDGNYEDAVKLSEEMAKKNGWVVVQDTAWEGYEEIPTWIMQGYGTIIY
                     EAMEQLKGYSIDKPTHVFLQAGVGSFAGAILGYLADKFGEDRPITVIMEPDAANCLYL
                     SSLSGKREIVEGDMPTIMAGLACGAPNTISYYILMDYADGFFSCPDYVAARGMRILAS
                     PLKGDPQVISGESGGAVGIGAVTLLMERDEYRDIRERLKLDETSVILIINTEGDTDPM
                     KFRDIVWDGDYSSVVGGVEG"
     gene            complement(2319..3071)
                     /locus_tag="PK768_02275"
     CDS             complement(2319..3071)
                     /locus_tag="PK768_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQA59827.1"
                     /translation="MKIVSFQLNGNVSYGVIENNEIMLIEGDIFGDFVVTDTRVSLDE
                     IKILPPVVPSKAVCIGLNYHDHIEETNSKVPDRPVVFIKPSTAVIGHMDDIKYPELSK
                     RVDYEGELAVVIGKKAKDINVNEAKEYIFGYTCGNDVTARDLQPADGQWTVSKSFDTF
                     LPLGPWIETELDPLNLEIRTFLNDELKQSSNTRHLIFDPYTLVSYISQIMTLLHGDII
                     LTGTPSGIGPMKKGDKVVVEIENIGSLINYVK"
     gene            complement(3263..4096)
                     /locus_tag="PK768_02280"
     CDS             complement(3263..4096)
                     /locus_tag="PK768_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HQA59828.1"
                     /translation="MAAKRMEGKLKSFDGTELFYRKDVPEKPKAIVVIVHGLCEHSGR
                     YDYVTEKFNEKGLGVYRFDNRGHGKSGGDRGYVEDFQHFFDDADKIVELALKEQKDLP
                     VFMLGHSMGGFIAAGYGMKYPGKLRGQIFSGPALLEIEALVKDIKKDDYFEKNPREKA
                     PNALSSLICRDTEVVKAYEEDPLVLKETNLKLLGEAFVKGPKWLAENVSKYEYPCLIL
                     HGEDDRIVPADSSKWLYENAPSKDKSIKIYEDCYHEILNEKEEKDEVINDIITWIEAR
                     I"
     gene            complement(4250..4552)
                     /locus_tag="PK768_02285"
     CDS             complement(4250..4552)
                     /locus_tag="PK768_02285"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQA59829.1"
                     /translation="MSFGLKQSDIEYIIGVIKKFPEIEKAVIFGSRAKGNYKAGSDVD
                     IAIYGENITFDTISSLHFMLEEEGPLPYFFDIVDYTHLEHRELKEHIDRVGKVIFE"
     gene            complement(4549..4944)
                     /locus_tag="PK768_02290"
     CDS             complement(4549..4944)
                     /locus_tag="PK768_02290"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HQA59830.1"
                     /translation="MDKDIRWKQRFANFKKATAQLTEFIQKGELNKFEVQGLIQCFEY
                     TFELAWKTMKDYLELEGFNVKSPRATIKTAFQIELIDDGHAWIDALEKRNLMAHTYDE
                     KRAMEAEKLIREKYYHIILKLRQKLEDLL"
     gene            complement(5234..6040)
                     /locus_tag="PK768_02295"
     CDS             complement(5234..6040)
                     /locus_tag="PK768_02295"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HQA59831.1"
                     /translation="MNRKRQLDIEKIADSVRAECKVTDYGIQNIFEAAEKLGYRVIRY
                     PLGNDAPLGFAIIKNTDRIIFSNSSSILSREIFSVAHEIGHHKLHLSEQGLTLIKDYD
                     FNDRDEYEVEANYFAACLLMPVEKVNSFIRFELNDKNINKWTGLDIARIQTAFNVSYD
                     MALIRLKSLGILNDDIKEKLKLEKLEKTASRLLKVINGNIDLCKATEVKRVPAEYLEW
                     VIFNYNEKLIPIKSLEAALNYVDLKVEDLDLSQEEHEEDESFDDLLKVMD"
     gene            complement(6037..6384)
                     /locus_tag="PK768_02300"
     CDS             complement(6037..6384)
                     /locus_tag="PK768_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA59832.1"
                     /translation="MNEVLGTRIRFLRESKGLTQEQVARQMNCSRQKYARIEKGLVDI
                     SYASIVTISKILGVKAEDITSAVNNINQKQVIYRRDGDSIREDKFNYIIEMIDTFYAH
                     RKLYNSVRQVNVE"
     gene            complement(6677..7438)
                     /locus_tag="PK768_02305"
     CDS             complement(6677..7438)
                     /locus_tag="PK768_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HQA59833.1"
                     /translation="MYKNTLKEKLQTGENVVGCIIQGAIPPLVEICGLVGFDFVFLDA
                     EHGPLSVRECEELIRAAEARNIIPLVRVRKNDPELILRYLDVGAMGIIIPGVRNKEEA
                     EQAVQAVKYYPRGNRGLSATRSSDYGLGMAMKDYVEYANQQTMVLAVIENVDAVNNIE
                     EILGVDGMDGAIIGTSDLAQSLGYPGQGKHPAVQEAFKKALEGGIRVGKPIGGVVRAG
                     ETAKTYFEQGCKMAVKSAYGIFAGAAKEFVRSAKS"
     gene            complement(7463..8965)
                     /locus_tag="PK768_02310"
     CDS             complement(7463..8965)
                     /locus_tag="PK768_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HQA59834.1"
                     /translation="MFELSNIVEGLNVLLSGSSMLAILIGTIIGIIVGALPGASATMA
                     IAVMIPITFWFSAEFSLLMLISVYCSGIFGGAITAILLNIPGTPASAATSFDGYPLAR
                     SGQGGKAIAVAIWSSFIGGLLSGIALLFAAPALATLALRFGPPESMMVALFGLTMVAS
                     LSSENLAKGLLMGALGMFMGCIGLDPTFGYARFTFGSPNLYSGIPVVPLLIGVFSVPE
                     VINMLTSKGWDKVVWDRKTKIRISFKEFKSIIKTCGVSAAIGMIIGLIPAIGPETATF
                     IGYDQAKKMSENKEMFGKGAIEGVAASECANNAVVGTSLAPMFALGIPGSGAAMVLMG
                     GLMIHGLMPGPRLFTEHKSLLMTIFLGFIVAQFFMLVGGLIAAKFSPAILNISHTILG
                     PSILIICMVGSFAVSNNIFDVYVMLIAGFISYFMGKAGFAPVPTVLGLILGPMFEGEV
                     GRTIRISSKTGFLPYILGRPITLLLAALTILTIALPIIFHKGGQKEVSIS"
BASE COUNT         2549 a         2070 c         1419 g         2989 t
ORIGIN      
        1 tgtatggaaa aattgctata cttgctgcgt cattgagaat aggcccgacg ctgtctagga
       61 ctacagttac ctttgagcct gcctttactt tgggcagatc agccgccctc atgccggaca
      121 atatcattat gtgactgctg tcatcgcctt tttcgggtat ttcctgttcg ggtggtgcgc
      181 ttactatttc cgctatccga ggccgttcat atgctaatac cttaaagtac ccaggcatcg
      241 accattctga ttcaacatcc tccatatccc agacttttac ccttacgtaa aaagcataag
      301 ttccggactg ccataatggc ccttcaatat gtggaagatt gctcgtgggt aaagtaaaac
      361 tagtttcgct gctatttatc tttcttgaat cgaatacaag gccatcatca gattgtcttc
      421 ttacttcaag ctggaaagcc gtctgatagt cgccttcatc ttcatcgaca aaatcccaat
      481 aaaaagtcgg tgtggtggta taataccatc cttcttccgg gacatgcaca atcggcggct
      541 ttggtttatt atttacttcc cactttacgg cagttcccgc tatgcctgtt ataaccgggg
      601 ttacatcttt gtttgtagtt gcaagttcag ctctccacat aaccgcattt ccgggaataa
      661 cttccgccca aagctctttt gtatcagacg tcacgcttgc tactgaaaca tctcctattg
      721 atctccagct atagccgtta tcatcgctta cttcaacgac cgttcctgtt tctatgcctc
      781 tagctctcca cctagttatc cagtttgccc cgtctgcagt tactgaccat gtaatacttg
      841 ttccagcagg tagtatcatg ttagccctga cccttaacat gtcagcttct gcagctcttg
      901 ttaatctatt ttccgcatct ctgccgattt gccccagttc tttaatgcga ttgaccaaac
      961 ggtttccatt tatacttttc atccttccac acctcccaca acagaactat agtctccatc
     1021 ccaaacaatg tctctaaatt tcataggatc tgtatctcct tcagtgttta ttattaatat
     1081 cactgaagtt tcatctaatt ttagcctttc tcttatatcc cgatattcgt ctctttccat
     1141 aagtagagtc actgccccaa ttcccacggc accccctgat tctcccgata ttacctgggg
     1201 atccccttta aggggtgatg ccaatattct catgcctctt gccgccacat aatctgggca
     1261 tgagaaaaag ccatcagcat aatccatcaa tatataatag cttatagtat ttggggcgcc
     1321 acaggccaag cctgccataa tagtaggcat atctccttct acaatctctc tcttgccact
     1381 taaggaagac agatacaggc aatttgcagc atccggctcc ataataactg ttattggcct
     1441 atcttctcca aatttatctg ccaaatagcc caagattgct ccggcaaagg agcctactcc
     1501 cgcttgaagg aatacatggg tgggtttgtc tatactatat ccctttagct gctccatagc
     1561 ttcatagatt atggtgccat atccctgcat aatccaggtg ggtatctctt catacccttc
     1621 ccaagctgta tcttggacaa ccacccaacc attcttcttg gccatttctt ctgaaagctt
     1681 aactgcatct tcataatttc cgtctataat agatgcatct gccccttctt ttcttatgtt
     1741 gtttaatctg gtaagggacg aaccccttgg catatagaca acggactttt gccctagttg
     1801 atttgctgcc catgccaccc ctctgccgtg gttgccatct gttgcagtga caaaggtaat
     1861 ttctcctatc ttttctttca cttctttaga gcgcaaatat tcaaaagata cttctgatat
     1921 atccatacct aatttattgg ctaaatattt gcctattgca taagcaccac ctaagacttt
     1981 aaaagcattt aatccaaatc tatatgactc atcttttaca aagatatttt ttaaaccaaa
     2041 gtgcttcgct aaagcatcta acttgtgcag cggcgtcacc gaatactcac taaagccttt
     2101 gtgaaaatca ttaacttttt taaccacttc ttcggaaata aaatctacac tggtctttgg
     2161 tttttctttt ctattatcaa ttagatttgc ttttataagc tcaatatttc ttgacatttt
     2221 tccccctcct tagtgatttt tttgggacgg gtacaaaata ttcatcatga tgaatatttt
     2281 gtacccgtcc caaaaaaatc acaaaaaatt caccaaaact atttcacata attaattaaa
     2341 ctgccgatgt tttcaatttc aacgacaact ttgtcgcctt ttttcatggg gccaattccc
     2401 gagggtgtgc cagtaagtat tatatctccg tgaagaagag tcattatttg tgaaatataa
     2461 ctaaccaatg tatatggatc gaaaatcaaa tgacgtgtat tagatgattg ctttaactca
     2521 tcatttaaaa aagttcgaat ttcaagatta agtggatcca attccgtctc tatccaaggt
     2581 cccaaaggta aaaaagtatc aaaagatttt gatacggtcc attgaccatc tgccggttga
     2641 agatcccttg cagttacatc attgccacag gtatatccaa aaatatattc tttagcttca
     2701 ttcacattta tatccttagc ttttttaccg attactacgg caagctctcc ttcataatct
     2761 actctcttgg ataattcagg gtactttatg tcatccatat ggccaataac tgcagtagaa
     2821 ggcttaataa agacaacggg cctgtccgga accttggaat tagtttcttc tatgtggtca
     2881 tgatagttta aacctataca aacagcttta cttggtacaa caggtggcaa tatttttatt
     2941 tcgtccaaag aaacccgtgt atctgttaca acaaaatcac caaagatatc tccttcaatt
     3001 aacataatct cattattttc aatgacacca taagacacat tgccatttag ctgaaatgat
     3061 actattttca tatctgcacc tccagataaa tcctaatcat tgagattttt caataactta
     3121 gatttattat agcatatggc tatttagccg catatttaaa aaatctgctt tagtttgaca
     3181 tttgttgtga acaatagggg acgggtacaa aatattcatc atgatgaata ttttgtaccc
     3241 gtcccctatt gttcattcta gtttagattc tggcttctat ccatgtgata atgtcgttta
     3301 ttacctcatc tttttcttct ttttcgttta aaatttcatg atagcagtct tcgtatattt
     3361 tgatagattt atcttttgag ggtgcatttt catagagcca ttttgacgaa tctgcaggaa
     3421 ctattctgtc atcttcgcca tggagtatca agcagggata ttcatatttt gagacatttt
     3481 ccgcaagcca cttgggtcct ttgacaaaag cttctcccag gagtttcaaa ttagtttctt
     3541 tcaataccag gggatcttct tcataggctt ttactacttc agtgtctcta caaatcagac
     3601 ttgatagagc attaggagct ttttccctgg ggttcttttc aaaatagtcg tcttttttaa
     3661 tgtcttttac caatgcttca atttccaata gtgccggacc tgaaaagatt tgccctctta
     3721 atttgccggg atatttcatg ccatagcctg cagcaataaa tcctcccatg ctgtggccca
     3781 acataaatac aggcaggtct ttttgctcct tcaaagcaag ttctacaatt ttgtccgcat
     3841 catcaaagaa atgttgaaaa tcttccacgt agccccggtc accgccggat tttccgtgac
     3901 cccgattgtc aaaccggtat actcccaggc ccttttcatt aaacttctcc gttacataat
     3961 catatcttcc cgagtgctca cacaatccgt gaactataac tactattgcc tttggctttt
     4021 ctggaacatc ttttctgtag aatagttctg ttccgtcaaa ggatttaagc ttcccctcca
     4081 tccttttagc agccatcaaa catcctcctt ttatcttgtc ttgctataat aaatcttatt
     4141 gtaattgagc ttttgtgtca agtccgattt ttctgtaata ttttgttgaa taaaatgccc
     4201 tgatgtttct gtttttcagc aaaacatcag ggccagtgtt tctcttctat tattcaaaaa
     4261 tgacttttcc aactctgtca atgtgctctt ttaattctct atgctctaga tgtgtgtagt
     4321 ccacaatatc gaagaaatac ggcaatggtc cttcctcttc aagcataaag tggagggagg
     4381 atattgtatc gaaagtaata ttttcaccat atatagctat gtcaacatca gaacctgcct
     4441 tatagtttcc cttagctcgt gagccaaata tcactgcctt ttctatttca ggaaactttt
     4501 ttatgacgcc aattatatat tcgatatcag actgctttaa accaaaactc atagcaaatc
     4561 ctccagcttt tggcgcagtt ttaatattat gtggtagtat ttctccctta ttaatttttc
     4621 tgcttccatt gctctttttt catcatatgt atgtgccatc aggtttctct tttctaatgc
     4681 atctatccac gcatggccat catctatcaa ctctatctga aatgctgttt ttattgttgc
     4741 tcttgggctt tttacattaa atccttccag ctctagataa tccttcatgg ttttccaggc
     4801 taattcaaaa gtatattcga aacattgtat caatccttga acttcaaatt tatttaattc
     4861 gcctttttgt ataaattccg taagctgtgc agtagctttt ttaaaattgg caaatctctg
     4921 cttccatcgt atatctttgt ccacagcttt cacctcacag tcaaaacaga gctttttctg
     4981 ataccatttt atctgataaa ggtgggttgt tcaatagctt ttcttattct tgccttaatt
     5041 gtttccataa aggccttttc cgtttcatca ctgaacagat tcactcacaa gttcatcttt
     5101 gatagatatg tacttagttt ttgataccga ataaattcat agacctttag tttgttaaat
     5161 ctattgaata aaatagcttg gaagctcgcc ttatataaat ggataataac attagtgtct
     5221 agtgatgcat ccattagtcc atcaccttaa gcaaatcatc aaagctttca tcttcttcgt
     5281 gctcttcttg agataagtct aaatcttcca ctttcaaatc cacataattt aaagctgctt
     5341 ccaagctttt tataggaatt agtttttcat tgtaattgaa aataacccac tctaaatatt
     5401 cagctggcac tctcttaact tccgttgcct tacaaagatc aatgtttccg ttgataactt
     5461 ttaaaagcct agatgcagtc ttttccagtt tctccaattt taatttttcc tttatatcat
     5521 catttagtat acctagtgat tttaatctaa tcagtgccat atcataacta acgttgaatg
     5581 ctgtttgtat tcttgctata tccaatccag tccatttatt aatgttctta tcatttagtt
     5641 caaatctaat aaagctattt accttctcaa cgggcattag caaacatgca gcaaaataat
     5701 tagcttcaac ttcatactca tctctatcgt taaaatcata gtccttaatt aatgtcaaac
     5761 cctgttccga caaatgcagt ttatggtgtc caatctcgtg agcaacagaa aaaatttccc
     5821 tagataagat tgatgatgaa tttgaaaaaa taattctatc agtgtttttt attatggcaa
     5881 atcccaaagg tgcatcatta cctaaaggat atctaattac tctatatcct aacttttcag
     5941 cggcttcaaa tatattttga ataccataat ctgtaacttt gcactcagct ctaacactgt
     6001 cagctatctt ttcaatatca agttgccttt ttctattcat tcgacattaa cctgccttac
     6061 actgttataa agttttctat gcgcgtaaaa agtatcaatc atttctataa tataattaaa
     6121 tttatcttcc cgaatagaat cgccatctct tctgtatata acttgttttt gattaatgtt
     6181 attgacagca gatgttatat cttcagcttt aactcccaga atcttggaaa tagttacgat
     6241 actagcatat gaaatatcta ctaaaccctt ttcaatcctt gcatattttt gtcttgagca
     6301 attcatttgt ctagcaacct gctcttgtgt caaaccttta gattctctca aaaatctgat
     6361 tcttgttcct aatacttcat tcatggcaac acctccctct caatttgatt ataatttatt
     6421 gtatgctaaa gtcaacaata atgttataat tttgtatcat ctatatctat tatttcttag
     6481 ttttggttga tttattcact acatgttaca tttgtttaac atgtatcatc agttaagctc
     6541 gctaaaaaaa cgcagttaaa aaatatatgt gaataaaaat aaataaaaag ggacgtgcac
     6601 aaaaaattca tcaaagtgaa tattttgtac ccgtcccctt ttattcaact atccactatt
     6661 tgcaaatgtg caaagtttag gatttggcac tgcggacaaa ttctttggcg gcaccggcga
     6721 atattccata ggctgatttc actgccattt tgcagccttg ttcaaaatag gtttttgccg
     6781 tttcacctgc tctgacaaca cctccgatag gctttccgac tcttatacca ccttcaaggg
     6841 ctttcttgaa ggcctcttgt acagccggat gtttcccctg accgggatag cccaaacttt
     6901 gggccaaatc actggtgccg ataattgcgc cgtccatacc gtcaaccccc aggatttctt
     6961 caatattatt tacggcatca acattttcga taactgctaa aaccatcgtc tgctggttgg
     7021 catactccac ataatccttc atggccatgc ctaatccata atctgatgat cttgtggcag
     7081 acaagcctct atttccacgg ggataatatt tgacagcttg tacagcctgt tcagcttctt
     7141 ctttatttct cacccctgga atgatgattc ccatggctcc aacatccaaa tatcgtaaaa
     7201 taagctctgg gtcatttttc cgcactcgaa ctaaaggaat gatattgcga gcttcagctg
     7261 ccctgatgag ctcctcacat tccctaacag aaagaggacc atgttccgca tcaagaaaaa
     7321 caaaatcaaa tcccaccagg ccacagattt ccacaagggg aggaatggct ccttggataa
     7381 tgcatcctac cacattttca cctgtttgaa gtttttcctt taatgtgttt ttatacacac
     7441 aaaccaccct cttatattaa ttttaagaaa tacttacctc tttttgacca cctttgtgga
     7501 aaataattgg caaggcaatt gttaaaatag tcaatgccgc caatagtaga gtaatgggac
     7561 gacctaggat atatggcaaa aatccggttt ttgaagagat tctgatagtt ctacctacct
     7621 ctccctcaaa cattgggcct aaaattagac ctaaaacagt aggcaccggc gcgaaacctg
     7681 ctttacccat gaagtaagaa atgaaacctg caatgagcat cacatataca tcaaaaatat
     7741 tattacttac tgcaaaagaa cctaccatgc agataattaa aatagatggc cccaatatgg
     7801 tatgggaaat attcagtata gcaggtgaaa actttgctgc aataagcccc ccaaccaaca
     7861 tgaaaaactg cgccacaata aagcccaaga aaatggtcat aagcagactt ttatgctctg
     7921 taaaaagccg cggccccggc ataagaccgt gaatcatcaa gccacccatc aataccattg
     7981 cagcccctga tcctggaata cctaaagcaa acatgggtgc cagagatgta cctacaacag
     8041 cattatttgc acattcagaa gccgccactc cttcaatggc gccctttcca aacatttctt
     8101 tgttctcaga catctttttt gcttgatcgt atccgataaa agttgccgtt tccggcccaa
     8161 tggcaggtat aaggccaatt atcattccaa ttgccgcaga aactccacat gtctttatga
     8221 tagacttaaa ctctttaaag cttattcgta tttttgtctt tctgtcccag actaccttat
     8281 cccaaccttt tgaagtgagc atattgataa cttctggaac agaaaacact ccaattaaca
     8341 gcggcaccac cgggataccg gagtagagat ttggacttcc aaaagtgaaa cgtgcatagc
     8401 cgaaagtggg atccagccca atgcagccca taaacattcc caatgcaccc atcaaaagtc
     8461 cttttgccag attttctgaa gaaagagagg caaccatagt taggccaaat aatgccacca
     8521 tcatactttc agggggaccg aagcgaaggg ctaaagttgc cagggcaggt gcggcaaaca
     8581 gcagtgcaat gccgcttaac aggccaccaa taaaggatga ccatatggca acggcaatcg
     8641 ctttgccccc ttgtccactt cttgccaaag ggtaaccatc aaaggaagtt gccgccgaag
     8701 ccggcgtccc tggaatattt agaagaatgg ctgttatggc tccgccaaat ataccgctac
     8761 agtatacact gattaacatc agtaatgaaa actctgcgga aaaccagaaa gttattggaa
     8821 tcattactgc tatggccatg gtggcagatg cccccggcaa tgccccaact ataatcccta
     8881 taatggttcc aattaagatg gccagcattg aagagcctga taatagaaca tttaagcctt
     8941 caacaatgtt acttaattca aacaaatatg ttcctccttg tataatgtat ttaggttatt
     9001 tattaggaaa acctataaac cttattc
//
LOCUS       DAONUO010000039         8932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_5981_length_8932_cov_3.732680, whole genome shotgun sequence.
ACCESSION   DAONUO010000039 DAONUO010000000
VERSION     DAONUO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5981_length_8932_cov_3.732680"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PK768_02315"
     CDS             <1..390
                     /locus_tag="PK768_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59835.1"
                     /translation="MSAYANIAEPGSEADPLVSRSYVDKVFHSLREYVDSKSGGSSSF
                     EIIYLEQGQVLIGNHSTEMILRSGIASAVDSGMGGLADVTAGKDIRHGENISQNHLLI
                     VPRDDGRGVRAENNNVVLMVRGGYTIQ"
     gene            492..1061
                     /locus_tag="PK768_02320"
     CDS             492..1061
                     /locus_tag="PK768_02320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA59836.1"
                     /translation="MERLTVLLALMALDAGGAETHAVSLAKQLKRRGVNVIVASHGGK
                     LTKVLETSDIPHYTLPLDRKSPLHLVKSVRGLTDIIKRYNVNIIHAHARIPAWISQWV
                     SYVTGCPYITTSHGIYSTGWGMGLITTWGRKVIAVSEDVKKHLVNGFRVSPDKIVVIP
                     NGIDLEQFDPQNIDSSLVEAQLGLKEDDL"
     assembly_gap    1204..1213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1214..1650
                     /locus_tag="PK768_02325"
     CDS             <1214..1650
                     /locus_tag="PK768_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA59837.1"
                     /translation="MADRFNLRSNQNKVIVTGARLDTPVFMNMADVAVGVGRVALEAM
                     AMEKPVIIAGEAGFMGILGPENFEIARKHNFSGRGADMKTDASNIADAIKTLLADGKI
                     RRELGSFGRQAVVRHFSIETMTDEVLKVYHQVIEEVNDEPNR"
     gene            1634..2119
                     /locus_tag="PK768_02330"
     CDS             1634..2119
                     /locus_tag="PK768_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HQA59838.1"
                     /translation="MSLIDNKGRVFGLINIIDLLVILLVVAVVGRFTLMQKQQSAGAV
                     TRTIEVVVHVKEVRDATCNVINEGDVVKETKTNAVLGKITNVKVVPSESIVETADGRL
                     VVAPNPVYKDIYLTIQGSGTAGDNAIVLGSNEIRVGTTLQLKTNMYSVTSTVMSIDVQ
                     L"
     gene            2179..4068
                     /locus_tag="PK768_02335"
     CDS             2179..4068
                     /locus_tag="PK768_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQA59839.1"
                     /translation="MLSLWIKNSLIMNMISLIFRPEVYKSSLFYKMISSLGTSITKAL
                     EGSFLRVFFTHKGWEPEAIRESRLMAAFQKISNPLNKVRHFLNTIVQNSFPYRFAKKA
                     TDSFVQAPMSTVAFMILPFMLSITILKGFYENFTPKSLLYRAILLVLLFFLLNVKVSL
                     KSLLDFSKTWKIFSWFCDVDLQHRESSGRALHYRDGLEFAAVSFTAGLLYYFLPQTTF
                     IKLFGIVFASTAIYIWPGLGLAMTAFMLPLLPTTYTVGIIGITFLSLLIKGDEINNTI
                     PAAIIPALLFMATAVIAAVFSVMRAESLKVLPLYAAYFMIFYTAAVLFRDSKTIKLVL
                     ASIIVSTVFLSLLGIYQYFFIKIPTAEAWVDVEQFPELATRVYATLENPNVLGEYLGL
                     SIPLLAGAFLASDRFRHKFILASVVCMLTLCLVLTFSRGAWLGLAISVLIFALLKEPR
                     LLALIVILALLSPMFLPPVVMNRIASIGSLEDSSNAYRITIWIAALRMIKDYWLTGVG
                     LGLTAFSRVYRDYMIAGASAVHAHNLYLEVCLELGVIGVFSLLWMVFRGFSEALSNVT
                     MNDKISYIAAGILAALAGHLFHGLFDYVWYSPRIVMAFWMYLGMMSALTSASFTGDDI
                     GERLS"
     gene            4065..5159
                     /locus_tag="PK768_02340"
     CDS             4065..5159
                     /locus_tag="PK768_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA59840.1"
                     /translation="MIRVLHVLTDTNIGGAGRYLFNLLSHRGDSPFEIIVACPGGGEL
                     ERQLRDKGIKVYTLQGGESSAKLGHIKRIRQIISREKVDIVHTHASLAGRIAGKLSGC
                     KVVMTRHGMGRGENGPAKRLITRIISKFFTDRIISISRAVKIDLIDSGVPADMISTIY
                     NGIDLSKFADIKGTLREKLGLSPEIPVVGIVARLVPEKGYEYAINAFYHVLKVFPSAR
                     LVIVGDGPLEESLKEMCTRLGITDNVAFMGYRPDVESLEADFDVFVLPSISEGLGLAL
                     LEAMALGKPAVATETGGIPEVIKHEANGMLVPPGNDKYLAEAIIKVLSDKELAKSLGL
                     AAQKTVFERFSSRTMIEKVEKLYMEILRRN"
     gene            5246..6367
                     /gene="csaB"
                     /locus_tag="PK768_02345"
     CDS             5246..6367
                     /gene="csaB"
                     /locus_tag="PK768_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HQA59841.1"
                     /translation="MKKGEKLMHRVLVSGYYGFGNAGDEAILIAIVESLKKLKENIEI
                     TALSADPLKTMEDYGINAVQRTNPFAIIREISRADLVISGGGGLLQDVTSNRSIPYYL
                     FILYLAKKLNKKVMFYANGVGPVFREMNKGMIKLVGNMVDLITVRDKQSKRELENLGV
                     TKPPMIVTADPAFVLKPIDDAVGYCLLEKLKISLREDHPRIGVSVRPWNLNKSREVIA
                     KACDYLVKSHNADVIFLPMQYPNDYFESLEVMKLMKEPATIVSEAMDPRELLWLCGKM
                     DIMFGMRLHALIFGAMNEVPLLGLVYDPKVEYFLERVEQLSAGKPGDLDLISLCNLLE
                     KTLSEIPKSKKILQEKREELKLLAEENAKLAVNLLEDIK"
     gene            6364..7125
                     /locus_tag="PK768_02350"
     CDS             6364..7125
                     /locus_tag="PK768_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HQA59842.1"
                     /translation="MKVGGRERIDILGVLLDRIDYHEACTRVENYLKSHDRKVIVTPN
                     AEIIMAARNNRDMMEVLNDADICLPDGIGVVIASRIFGKPLQERTTGFDFMIKILKLA
                     EIQGLSVFLLGGKVGVAEQAAAKIRELFPGLEIAGTHHGYFKDEEKDEIIDIINDKEP
                     DILLVAMGFPKQEKFMIENKDKLKFRVAMGVGGSFDVLSGNVSRAPVFMQKAGLEWLF
                     RLITQPWRFKRMMVLPLFLIEVVIHRLFSNKVRLR"
     gene            7138..7989
                     /locus_tag="PK768_02355"
     CDS             7138..7989
                     /locus_tag="PK768_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQA59843.1"
                     /translation="MKLRALFLDYAQIFIGSLIAALGLTMFLVPNKLAAGGVSGIATV
                     LHYVFDVPVGWTMLGLNIPLFVAGVALLGKSVGIKTIIGGLLFSVFTEITAGFPVPTQ
                     DLILSAVYGGIVLGIGLGLVFRGRGSTGGTDLAAMILHHFVPSISEGQGILVIDFFVI
                     ILSGLAFNWELAMYAWISLFVSSKVIDLILEGFNYAKAVYIISNKPEELSRKILTELG
                     RGVTLFRAKGGYSLKEKNVLLCVITRFEIAKLKSLVHEADPKAFVIIHDVHEVLGEGF
                     SFQADDN"
     gene            8014..8835
                     /locus_tag="PK768_02360"
     CDS             8014..8835
                     /locus_tag="PK768_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQA59844.1"
                     /translation="MSISNQLIDELQAKARIIRRHIIEMVAEAGSGHPGGSLSSADIV
                     TALYFHFMNIDPKRPDWPDRDRFVLSKGHAAPVLYAALAEKGYFPKEELLKLRKTGAM
                     LQGHPDMKTTPGLDMTTGSLGQGLSAANGMALAGKLDNKDYRVYVLMGDGEIEEGQIW
                     EAAMAAAHYKLDNLTGFVDYNGLQIDGPINQVMSPESIQEKFKAFRWHVIDIDGHDMK
                     QIIEAVEEAKTIKGKPTVIVAKTVKGKGVPFMENQVGWHGKAPSLEQAKEAIEAL"
BASE COUNT         2525 a         1707 c         2268 g         2422 t
ORIGIN      
        1 atgagtgctt atgcaaatat agcggaaccg ggctctgaag ctgaccccct tgtcagcagg
       61 agctatgttg ataaagtgtt ccacagtctc agggagtacg tggattccaa atccggtggt
      121 tcctcgagct ttgaaataat atatttggaa cagggtcaag ttttaattgg caatcacagt
      181 acggaaatga ttttaagaag cggaattgcc tcagctgtag acagcggaat gggcggtttg
      241 gcagatgtga cggcaggtaa ggacataaga catggagaga atataagtca aaaccacctt
      301 ctcatcgtac cgagggatga tggtaggggc gtaagggctg agaacaataa tgtggtgttg
      361 atggttagag gtggatatac tatacaatga caagctagct caagttttgc tgaaaagctg
      421 tgaagcttta cataagcact gtatgatgtt atactttttt tagcaaattc tcggagagag
      481 aggctttttt aatggaaaga cttacggttt tattggcttt aatggcactt gatgccggcg
      541 gagcagaaac tcatgctgtc tcccttgcca aacaattgaa aagacgaggg gtaaacgtga
      601 tagttgcttc acatggcgga aaattgacga aagtccttga aacaagcgac ataccccatt
      661 acactttgcc ccttgacaga aaatccccct tgcacttggt gaaatctgta agaggcctta
      721 ctgatattat aaaaaggtac aacgttaata ttatccacgc tcatgctcga ataccagcct
      781 ggatttctca atgggtgagc tatgtcaccg gctgtcctta cataacaacg tctcacggaa
      841 tttattctac cggctgggga atgggcctta ttacgacatg gggccgcaag gtcatcgccg
      901 taagcgagga tgtgaaaaag cacctggtaa acggctttag ggtgagtccc gataaaattg
      961 tggtgattcc aaacggtatc gacctggagc agtttgatcc ccaaaacatc gattcatctt
     1021 tggtggaagc acagttaggc ttaaaagagg acgacttata aatcatatac atcagccggc
     1081 taatgggcgc caggggggaa attgccttaa gattaattga agccatgccg gaaattgcaa
     1141 aagtctttcc ccgggtaagt ctcgtaattg tgggcgaagg cgacaagttc gaagctatta
     1201 aacnnnnnnn nnnagatggc ggataggttc aatttaagat caaatcaaaa caaggtaatc
     1261 gtcaccggtg cccgtcttga tacccccgtt ttcatgaaca tggcggatgt ggctgtcgga
     1321 gtaggccgtg tggcacttga agccatggcc atggaaaagc ctgtaatcat tgccggtgag
     1381 gcaggattta tgggcatatt gggaccggaa aactttgaaa tagcgaggaa gcataacttc
     1441 agcggccggg gggcagatat gaagaccgat gcctcgaata tagccgatgc cataaagaca
     1501 ctgctggctg atggtaagat cagaagggaa ttggggagct tcggccgaca ggcagtagtc
     1561 aggcattttt ccatagaaac tatgaccgat gaagttttaa aagtatacca tcaggtaatt
     1621 gaggaggtca atgatgagcc taatagataa caaaggcagg gtatttggtt taattaacat
     1681 catagactta ctggtaatct tattggttgt agctgttgta gggcgcttta ccctaatgca
     1741 gaagcaacag tcggccggtg ccgtcacaag aaccattgaa gttgtagttc atgtgaagga
     1801 agtaagggat gccacctgca atgttataaa cgaaggagat gttgtgaaag agaccaaaac
     1861 caatgcggtt ttgggcaaaa taacgaatgt gaaagttgta ccctctgaaa gcattgttga
     1921 aactgctgac ggacgcttag tggtcgcacc caacccggta tacaaggata tctatctgac
     1981 aattcaggga tcgggaactg ccggcgataa tgctatagtg ctgggcagca acgagattcg
     2041 agtgggtacc acactgcagc tcaagacaaa tatgtattcg gtgacatcca ccgttatgag
     2101 tatagatgtt caattatgac agattcaagc taatatggct tgtgtacagt atgaatcctt
     2161 actgtaagga gattcgcaat gctcagtttg tggattaaaa acagccttat aatgaacatg
     2221 ataagcctca tatttagacc ggaagtctat aagagcagcc tgttttataa aatgatatct
     2281 tccctcggca catcaattac caaagcactt gagggcagct ttctcagggt attttttacc
     2341 cataagggct gggaacctga agccataaga gaaagtcgat tgatggcagc cttccaaaaa
     2401 atcagtaatc ccttaaacaa agttcgacac tttttaaaca ctattgttca aaacagcttt
     2461 ccctatagat ttgcaaaaaa ggcgactgat tcatttgtac aggctcccat gtcaacagtg
     2521 gccttcatga tactgccttt tatgctgtca attacgatct taaagggctt ttatgaaaat
     2581 ttcactccaa aatctctgtt gtaccgcgca atcttactag ttttattatt ttttttgcta
     2641 aatgttaagg tttctcttaa gtcactgcta gattttagca aaacttggaa aatctttagt
     2701 tggttttgtg atgtcgatct gcagcaccgg gaaagctccg gaagagctct gcattacagg
     2761 gatgggctag aatttgctgc agttagcttc acagcaggat tgctgtacta ctttttgcct
     2821 caaacaactt ttatcaaatt gttcggcatt gttttcgcat ctacggcaat ttatatatgg
     2881 ccgggcttgg gccttgccat gacggcattt atgctgccct tgctgccgac cacatatacc
     2941 gtgggcataa tagggataac ctttttatcc ctgctcataa aaggagatga aataaataac
     3001 accataccgg ctgccataat accagctttg ctgtttatgg caactgcagt aatagcggcg
     3061 gtgttttcgg tgatgagagc agagagcctt aaagtccttc ccctctacgc ggcatatttt
     3121 atgatctttt acactgctgc agtattgttt agagattcaa aaaccataaa gctggtgcta
     3181 gcttccataa tcgtttctac agtctttctt tcgctgctgg gcatttatca gtattttttt
     3241 atcaaaattc caacagccga ggcttgggtt gatgttgagc agtttcccga acttgcaacc
     3301 cgggtctatg caactcttga aaacccgaac gttctgggag agtatctggg tctctcaatt
     3361 ccgctgctag caggtgcttt cctggcatcg gatagatttc ggcacaaatt cattttggcc
     3421 tcggttgtgt gcatgctgac actatgcctt gtcttgacct tttcaagggg agcctggctg
     3481 ggattggcta tatcggtctt aatctttgct ttgctgaagg aaccacgact cttggcgctg
     3541 attgtcatat tggccctttt gtcacccatg tttttaccac cggttgtaat gaatcggata
     3601 gccagtatag gcagcctgga ggatagttct aatgcctatc gaataacaat ttggattgca
     3661 gctttgagaa tgataaaaga ttactggctt accggtgtgg gattgggcct tacggcattt
     3721 tcaagggtat acagggatta tatgatagcg ggggcatcgg ccgttcatgc gcacaattta
     3781 tacttggaag tttgccttga gctcggggta ataggagtat tttccctcct ctggatggtt
     3841 ttcagaggct tttccgaggc cctaagcaat gtgacgatga atgataaaat atcatatatt
     3901 gcggctggga tccttgcagc cttagccgga catctgtttc atggattgtt tgattacgtg
     3961 tggtacagtc ccagaatcgt catggctttt tggatgtacc tagggatgat gtcagccttg
     4021 acatcggctt cctttaccgg tgacgatata ggggagaggc tatcatgatt agggtccttc
     4081 acgtcctaac ggatacaaat attgggggtg cgggcagata tctcttcaat ttgctgtcac
     4141 accggggaga cagtcctttt gagattatag ttgcctgccc tggcggtggt gagctggaac
     4201 ggcagttgag agataaaggc ataaaggtat ataccttgca agggggagaa agttccgcaa
     4261 agctagggca cataaaaagg attaggcaga ttatttcccg ggaaaaagtg gacattgtac
     4321 atacacatgc aagcctcgct ggtcggattg ctggtaaatt atcagggtgt aaagtggtta
     4381 tgaccaggca cggaatgggc aggggtgaaa atggacctgc taaaaggctt ataacaagga
     4441 ttatttcaaa gttttttaca gaccgaataa tatccatatc tcgggccgtg aagattgacc
     4501 ttatagacag cggagtccct gcggatatga ttagcacaat atataacggc atagatctat
     4561 caaaattcgc agatatcaag gggaccttgc gggagaaact cggcctttcg cctgagatac
     4621 cggtagtggg aatagtggcg aggcttgtgc cggaaaaggg ttacgagtac gctataaatg
     4681 ccttttatca tgtgctcaag gtttttccat cagcccgtct cgtgattgta ggggatggcc
     4741 cccttgaaga atcattaaag gagatgtgta cgagactggg cataacagac aatgtagcct
     4801 ttatgggcta cagaccagat gtggaaagcc ttgaagcgga ttttgacgtg tttgtcctcc
     4861 cttccatcag cgaaggtttg ggcctggctt tgctggaggc aatggccctc ggaaagcctg
     4921 ctgtcgctac ggaaacgggg ggtatacctg aagttataaa acacgaggct aacggcatgt
     4981 tggtgcctcc gggcaatgat aagtatttag ccgaagccat cataaaagta ctatcggaca
     5041 aagagctagc aaagagcctt ggccttgctg ctcaaaagac ggtttttgaa agattttctt
     5101 caagaaccat gattgaaaaa gttgaaaagc tatacatgga gattctccgg aggaattgag
     5161 tcaatttgcg gcaggcagtg ctgcgtgaaa gctataaggc tgttaataga tgctgggttt
     5221 gtatcaatta gggggattta tctctatgaa aaaaggagaa aaactgatgc acagagtatt
     5281 ggtttccggc tattacggtt tcggtaatgc tggggatgaa gccatactta ttgctattgt
     5341 ggaatctcta aaaaaattaa aagagaatat tgaaattact gccctttcag cagatcctct
     5401 aaagaccatg gaggattacg gcataaatgc tgttcaaagg actaatccat ttgcaataat
     5461 aagggagata tctagggctg acctggtaat cagcggagga ggaggactcc tgcaggatgt
     5521 taccagtaac cgttcaattc cctattatct gttcatactg tacctggcta aaaagctaaa
     5581 taaaaaagtg atgttttatg ccaatggtgt aggaccggtc tttagagaaa tgaacaaagg
     5641 catgattaaa ctcgttggaa atatggtgga ccttataacc gtaagagaca aacagtccaa
     5701 aagagagctg gaaaatctcg gtgttaccaa gcccccgatg attgtcactg cagatccggc
     5761 atttgtactc aaacccattg atgatgctgt ggggtactgt ctgcttgaaa aactcaagat
     5821 tagccttagg gaggatcatc cgagaattgg agtttccgtg agaccctgga atttaaataa
     5881 gagcagggag gtaattgcta aagcatgtga ctacctggtt aaaagtcaca atgcggatgt
     5941 tatatttttg ccgatgcaat atcccaatga ctacttcgag tcccttgaag ttatgaaatt
     6001 aatgaaagaa cctgccacaa ttgtctcaga agccatggac ccgcgggaac ttttgtggct
     6061 ctgtggcaaa atggatatca tgttcggaat gcggctccat gctctgatat ttggtgccat
     6121 gaatgaggtg cccttgctgg gtctcgttta cgatcctaag gttgaatatt ttctggagcg
     6181 ggtggagcaa ctttccgccg gcaaaccagg tgatttggac ctaatttctc tctgcaattt
     6241 gttggaaaag acattaagtg aaataccaaa gagcaagaag attcttcagg agaaaaggga
     6301 agagctgaag ctgctggcag aggaaaatgc gaaactggcc gtaaatctac tggaggatat
     6361 aaaatgaagg ttggcggtag ggaacggata gatatactcg gcgtattact tgaccgtatt
     6421 gattatcatg aagcttgcac gagggtggag aattatttaa agtctcatga taggaaggtg
     6481 attgtaacgc ccaatgcgga aataatcatg gctgcacgta ataaccggga tatgatggaa
     6541 gttctaaatg atgcagatat ttgtctgccg gacggcattg gtgtagtcat agcatcccgc
     6601 atttttggca agccgctgca agagcggact acgggttttg acttcatgat aaaaatactg
     6661 aaacttgcag aaattcaggg cttgagtgtg tttttgctgg gaggtaaagt tggtgtagcg
     6721 gagcaggcgg cagctaaaat acgggaattg ttcccgggcc tcgagattgc cggaactcat
     6781 cacggctatt ttaaagatga agagaaagat gaaataattg acataataaa tgataaggag
     6841 cccgacattc tgctagttgc gatgggcttt cctaaacagg aaaaattcat gattgaaaac
     6901 aaagacaagc tgaaatttcg cgtggccatg ggtgtaggag gcagctttga tgtactttcg
     6961 ggcaatgtga gcagagcgcc ggttttcatg caaaaggcgg gtctggaatg gcttttcagg
     7021 ctaataactc aaccatggcg atttaaaagg atgatggtgc tgcccctttt cttaattgaa
     7081 gttgtaatac acagattgtt ttctaataaa gtcagattaa gataaagaag tgagtctatg
     7141 aaactcagag ccctgttcct ggattatgca cagatattca tcggttcctt aatcgcagcc
     7201 ttaggcctta cgatgtttct tgtgccaaat aagttggcgg caggtggtgt cagcggtata
     7261 gcgactgtat tgcactatgt ctttgatgtg ccggtaggct ggaccatgct gggccttaat
     7321 atccctcttt ttgttgcggg agtcgcttta ttaggcaaaa gtgtcggaat aaagacaata
     7381 attggcggcc tgctgttctc ggtgtttacg gagattactg caggttttcc ggtgccaacc
     7441 caagacttaa tcctttctgc agtatacggt ggcatagtgc ttggaatagg tctgggcctt
     7501 gtgtttaggg gaaggggctc tacgggtggg actgatttag cagcaatgat tttacaccac
     7561 tttgtgccat ctataagtga ggggcaggga atattagtca tagatttttt tgtaataatt
     7621 ttaagtggcc ttgcctttaa ttgggaactg gcaatgtatg cctggatttc cctgtttgtg
     7681 agcagtaagg tcatagattt gatactcgaa gggtttaatt atgccaaagc cgtttatatc
     7741 atttcaaata agccggaaga gctgtcacgc aaaatattga cggaactggg caggggagtg
     7801 acccttttta gggctaaagg cggttattcc ctaaaagaga aaaatgtcct gctatgtgta
     7861 ataacacgct ttgagatagc taaactaaaa agcttggtcc atgaagcaga ccctaaagct
     7921 tttgtgatca tccatgacgt ccacgaggtt ttaggtgaag gctttagttt ccaagctgat
     7981 gataattaac tttgtaagga gtgatcgttc actatgagca tctcgaatca acttatcgat
     8041 gaacttcagg caaaggcccg catcattaga cgacatataa ttgaaatggt tgccgaagcc
     8101 ggatcaggcc atccgggcgg gtccctttct agtgccgata ttgttacggc cctgtatttc
     8161 cattttatga atatagatcc aaaaagacct gattggcctg acagagatag atttgtgcta
     8221 tctaagggtc acgccgctcc cgttttatat gctgcattag cagagaaagg ctattttcca
     8281 aaggaggagc tgttaaaact aagaaaaacc ggggcgatgc ttcagggaca cccggatatg
     8341 aaaacaactc ccggccttga tatgactacg ggttctctag ggcagggatt gtctgccgca
     8401 aacggcatgg ccctcgcggg aaaacttgac aataaagact accgggtata tgttttgatg
     8461 ggggacggtg agatagaaga aggtcagatt tgggaagcag ctatggcagc agctcattac
     8521 aagttggaca accttactgg attcgtagat tacaacggcc tgcaaatcga cggccccatc
     8581 aaccaggtca tgtcacctga aagcatacag gaaaagttca aagcctttcg ctggcatgta
     8641 atcgacattg acggccatga tatgaagcag atcattgagg ctgtcgaaga agcaaagact
     8701 attaagggca agcctaccgt aatagttgca aaaaccgtta aaggtaaagg cgtgcctttc
     8761 atggaaaacc aggtaggatg gcatggaaag gcaccaagct tagaacaggc aaaagaagcc
     8821 attgaagctt tataagtata tgggaggaga tttagatgac gaagatagca acccgtgatg
     8881 cctatggcga ggcactggca cagctgggtg aagagataaa ggatattgta gt
//
LOCUS       DAONUO010000040         8864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6041_length_8864_cov_3.873993, whole genome shotgun sequence.
ACCESSION   DAONUO010000040 DAONUO010000000
VERSION     DAONUO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6041_length_8864_cov_3.873993"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /gene="smpB"
                     /locus_tag="PK768_02365"
     CDS             complement(<1..296)
                     /gene="smpB"
                     /locus_tag="PK768_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQA59845.1"
                     /translation="MENKSRVLVTNRKARHDYHVLETFEAGIALVGTEVKSLREGKGN
                     LKDSFAKVENGELFLYNMHISPYEKGNIFNRDPLRTRKLLMHKKQINRLAGRVQ"
     gene            complement(437..847)
                     /locus_tag="PK768_02370"
     CDS             complement(437..847)
                     /locus_tag="PK768_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQA59846.1"
                     /translation="MTFDDGLLAENPQAPGLIVISGTFNGNIYNGSIVYVTSNGSFKG
                     NIITKNLIIAGKVTGSVQVENLKICDSGKLFFDKIQYDNLCIEEGGLLIEAPGNVEVK
                     SPSARFNVSIPISKETGKKETIGSDELLHFDCSY"
     gene            complement(870..1886)
                     /locus_tag="PK768_02375"
     CDS             complement(870..1886)
                     /locus_tag="PK768_02375"
                     /inference="COORDINATES: protein motif:HMM:NF037161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HQA59847.1"
                     /translation="MTQLIGIDVGYGFVKVTDGQVGYSFPSVVGEGHTKPTFSMKIGT
                     SPAIDQLRIGIGDRLFFVGKSAIKHSRFAYRDLSYTRSISDDLEILFFSALSLFIKDA
                     VNAFNVVTGLPVERMHLAEDLIKRVKGERTVTLYNGASSRNTTINVNEIEVVPQPLGT
                     YWSSLSTSAIPADGRIGVVDIGFKTTDFATIEDSEYVPEKSTSIPVGLATAYDDIGSN
                     LITEFGIEKESHALDGIVINRKINISGETIDISNIIESAFEKLATNILVEIHSLWNTS
                     EFDTLLLTGGGGQALSSYLLPQLSHAKLSSDPITANCRGYLTWANRLWNQVLPQTNGT
                     LKFD"
     gene            2088..2564
                     /locus_tag="PK768_02380"
     CDS             2088..2564
                     /locus_tag="PK768_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HQA59848.1"
                     /translation="MCKVLIIGMGNLLMSDDGLGLVALYELRKSYEPRPEVCYLEMGT
                     SVLNYVTDIGKAETLIAIDAITAGHDPGTLYRAYDSDIDMVDRRPFFLNSHGASLREA
                     VALSRELTGLPSRIIIYGIEPDTCEFGFFISPRVKKSLKRLVEIVQKDIDCILNSR"
     gene            2570..2920
                     /locus_tag="PK768_02385"
     CDS             2570..2920
                     /locus_tag="PK768_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HQA59849.1"
                     /translation="MKLLHELSITSDILDLVLCELAKKGYNKVIKIDITIGDLSGIVR
                     EHIKYYFELLSRATPAAGATLTINYKKSKFRCESCKIIYESCDFSFLCPVCKSRGTLV
                     ESCASIYINSIEVE"
     gene            2920..3570
                     /gene="hypB"
                     /locus_tag="PK768_02390"
     CDS             2920..3570
                     /gene="hypB"
                     /locus_tag="PK768_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HQA59850.1"
                     /translation="MKIKVLKDVLEANNYIARKIREELAKRQAMMINIIGSPGAGKTS
                     FIIKTIEVLDVKAVVIEGDVTSDIDSRKVASFGIPVVQVNTGGGCHLNANMIDLALKK
                     IPVENAVIFVENVGNLICPASYDIGEDFKLAICSVPEGHDKPFKYPAIFEKAGAIVLN
                     KVDLIKHVDFDRDTFYEGVKTLNPKVSVFEVSSINGDGIEEWGQWLKSQFMEKINR"
     gene            3531..5261
                     /gene="hypF"
                     /locus_tag="PK768_02395"
     CDS             3531..5261
                     /gene="hypF"
                     /locus_tag="PK768_02395"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HQA59851.1"
                     /translation="MAQVAVHGKNQSLICYQAQDEVLHDYEAICKAVDMIKQGKIIAV
                     KGIGGYHLAVDARNEAAVSRLRQKKHRYGKPLAVMMLNTTEVEKYCEISISERQILES
                     QAAPIVLLKRRKGGAPLAYSLTKGLDTLGVMLPYTPVHKTLMEKLGFPLVITSGNISD
                     EPICICEEEAHSRLKNVADGFLHHDRPILNRIDDSVCFFAAAGVRMVRRSRGFANAPI
                     MLDTPSKPVLACGAFYKNTFCLFDDNKAYVSHHIGDLDGSLAYCYFKEEIQKYKRKYN
                     INPSLVVRDLHPSYLSSLYADELGLPCLKVQHHHAHIASVMAEYGLREKVLGIAYDGT
                     GLGSDGKIWGAEFLLADLKDFQRLGHLKYVPLPGGDLAVKHPFRTALGFIYPNMHRFS
                     KYIERLDGEQVKIIINQIERKVNAPLASSMGRLFDAVASIINLRDTVDYEGQAALELE
                     SLILPTNDCYGYRIEREGTGFFIDVDVLLEELYTDYINGVPQGIISAKFHNTVINFTL
                     DLAKRIRDMNNVGKVVLSGGCFQNRYLLEGLNRELQGEGFAVYIPSKIPVNDGGISLG
                     QAAIASTYFS"
     gene            5278..5490
                     /locus_tag="PK768_02400"
     CDS             5278..5490
                     /locus_tag="PK768_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HQA59852.1"
                     /translation="MCLAVPMKVKNIRWPNAEVLAEGLRREVNIQLVPRVNIGDYVLV
                     HAGFAIQVIDGKWAKENLELWEQIKS"
     gene            5469..6503
                     /gene="hypD"
                     /locus_tag="PK768_02405"
     CDS             5469..6503
                     /gene="hypD"
                     /locus_tag="PK768_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HQA59853.1"
                     /translation="MGTNQVLRSVNTELKIMEVCGTHSEVVSKYALRQFYTGRVKLVS
                     GPGCPVCVIASEDIDLIIEYARCGFTIVTFGDLIRIPGTVSSLAVELAHGKSIKVVYS
                     PLDALAMAKDNPSNQVIFVAVGFETTAPLVALTIMKAKKEGIKNFSVLSLHRTIPQIL
                     RTLFQTGVNLDGLILPGHVCTITGSKPFEFLARDFGITAVISGFAPEDIMESIEIIRE
                     NLHRPSIYIQYKRAVSTSGNMVAQEAIKKVFKPCDALWRAFGLIKDSGLAIKEAWSFF
                     DARKRWPIKPIHHKQALNKKCSCKDIMKGILTPLECPLFRTFCRPESPIGPCMVSSEG
                     SCAIYYKYGG"
     gene            6507..7505
                     /gene="hypE"
                     /locus_tag="PK768_02410"
     CDS             6507..7505
                     /gene="hypE"
                     /locus_tag="PK768_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HQA59854.1"
                     /translation="MDIVLSAHGGGGKLTEELIQEVFLNEFDSDVLNEMEDSAVLDID
                     FNKLAFTTDSYTVKPLFFPGGDIGRLAVCGTINDLLTRGAVPLFLSASFIIEEGFRLY
                     DLKRIASSMARTCREAGVKIVAGDTKVVAMGDADGIFINTSGAGYVPFGVNISVKNAK
                     PGDKVIISGTVGDHGMAVLSSREDFTFDPPLESDCAPLADIVNTLRSFGESVRVLRDP
                     TRAGVAEVLYNISRASGVGIEIYEEKLPVKPQVASACNMLGLDFLQLANEGKLVAVVE
                     SSAAVKILEAIKKCKNGREAAIIGTVNDSGQITARTALGTSRILSRPVGELVPRIC"
     gene            7592..>8864
                     /locus_tag="PK768_02415"
     CDS             7592..>8864
                     /locus_tag="PK768_02415"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQA59855.1"
                     /translation="MAENIEKASVKIKGMTCAACAARIQKVVGKMPGVQDASVNFATE
                     KLNVTFEPEKVSVEDFIKKVRDAGYDVVMDKAELGLKGMSCAACAARIEKALSKAPGV
                     YKATVNFATETATVEFNSSETSLKNLIKVVRDAGYDAFEKTDVNIDREKEEREREIKN
                     LGSLAVISAILSAPLLFGMFLHMMGMSGGILFNPWFQLAVSAPVQFIIGFRYYKGAYH
                     ALKGGGANMDVLVAMGTSAAFFYSLYNAFTVPAEQIHHYLYFEASAVVITLITFGKYL
                     EAVAKGRTSEAIKKLMGLAPKTARVIRGSEEIDIPIEEVEVGDIVVVRPGEKIPVDGV
                     ITEGYSSVDESMLTGESIPVEKHVGDEVVGATINKTGTFKFKATKVGKDTVLSQIIKL
                     VEEAQGSKAPIQKLADQISGVFVPVVVVVAAI"
BASE COUNT         2623 a         1764 c         2064 g         2413 t
ORIGIN      
        1 tcctgaaccc gtccggcaag tctgttaatt tgctttttat gcatcaataa tttgcgcgtt
       61 cttaagggat ctctattgaa gatattcccc ttttcatatg gacttatgtg catattatat
      121 aaaaacaatt caccgttttc aactttcgca aaactgtctt ttagattacc cttgccttca
      181 cggagcgatt taacctccgt tcccaccaaa gcgataccag cctcgaaggt ttccaataca
      241 tgataatcat gtctagcttt tcgatttgtt accaggactc gggatttgtt ttccaacgct
      301 caaacctcca cttgatggaa atctgtttac acctcatatt ataacatact gtagtatagc
      361 cgcaatcata aactataaaa acgagggttg ccaaataaat aaaaagccgc ttgagtggct
      421 ttatacttaa aagcatttaa taactgcaat caaaatgaag taattcatca gaaccgattg
      481 tttctttttt accagtctct tttgagattg gaatactgac attaaatctt gccgagggag
      541 attttacctc aacatttccg ggagcttcta ttaacagtcc accttcttca atacacaaat
      601 tgtcatattg tattttgtca aaaaacaatt tgccggaatc acagattttc aggttttcta
      661 cttgaacact gccggtgact tttccggcga taatcaagtt tttggtaatg atattccctt
      721 taaaactacc gttggaagta acatatacaa tgcttccatt gtaaatattg ccgttaaaag
      781 taccgcttat cactatcaaa ccgggtgctt gtggattttc tgccaacagt ccatcgtcaa
      841 atgtcatgat ttagtcactc cacattaccc tagtcaaact ttaaagtgcc gttagtttga
      901 ggtaaaacct gattccacaa tctgttagcc caagttaaat agccacggca gttggcggtt
      961 atcgggtccg aagaaagttt tgcatgagat aattgaggaa gcaaatatga actcaaagct
     1021 tgtccgccac caccggttaa taaaagggta tcaaattccg atgtgttcca tagggaatgg
     1081 atttcgacga ggatgtttgt agctaacttt tcaaatgcac tttcaatgat gttggatata
     1141 tcgatggttt ctcccgaaat attgattttc cgattgatga caattccatc caaggcatgg
     1201 ctttcctttt caatgccaaa ttcggtgatc agatttgaac caatgtcgtc ataagcggtt
     1261 gccaaaccaa ccggtataga tgtacttttt tcgggtacgt actccgaatc ctctatagtc
     1321 gcaaagtcag tagttttgaa gccgatatct actactccaa ttcgtccgtc tgctgggatg
     1381 gcagatgttg agagagaaga ccaataggtc cccaaaggtt gcggtaccac ctctatctca
     1441 tttacattta tggtggtgtt gcggctagat gcaccattat aaagggttac agttcgttca
     1501 cccttcactc gttttatcaa gtcttctgcc aggtgcatcc gttcaactgg gagaccggta
     1561 acgacattaa aggcatttac ggcatctttg ataaaaagac ttaaagcgct gaaaaaaagt
     1621 atttccaagt cgtcacttat ggagcgggtg taggaaaggt cacgataagc gaacctagaa
     1681 tgtttaatgg cagattttcc aacaaaaaag agcctgtcgc ctatgccgat tcgaagttgg
     1741 tcgattgcgg ggctagtgcc tattttcatg ctaaaagttg gttttgtatg accttctcct
     1801 acgacactgg gaaaggagta accgacctgg ccatcagtta ctttgacaaa accgtaaccc
     1861 acatcgatac caattagctg tgtcacaaag aaacctcctt tttatgtttt aatgtgcgcc
     1921 cttagtcttt tgccccactt ttaacaatat aataccaatt attcaatatt ttgtaaacaa
     1981 gttgatgaat cgtgtcgttt aaggacgttt atggataaaa ttagcagaaa tgtggaagaa
     2041 ttatttaata atgactttta ttatgtagaa gaaagtaggc aaggcttttg tgcaaagttt
     2101 taattatcgg catgggcaat ctattgatgt ctgatgacgg tctaggcctt gttgctctat
     2161 atgaattacg taaaagctat gagccgcgcc ccgaggtttg ttatttagag atgggcacat
     2221 ctgtattaaa ctatgttact gatataggga aagcagagac cttgatagcc atagatgcga
     2281 taacagcagg tcatgatccc gggaccctat accgcgcata tgatagtgac attgatatgg
     2341 tagaccgaag gccatttttt ttaaacagtc acggggccag tctccgggag gcggttgcac
     2401 tttccagaga actaacgggt ttgccgtcaa ggattatcat atacggtatc gagccggata
     2461 cctgtgaatt cggttttttt atctcgcccc gggtgaaaaa atcactaaaa aggctcgtag
     2521 agatagtgca gaaggatatc gattgtattt taaatagcag ataaagcagg tgaagctttt
     2581 gcatgaactt tcgattacat cagatattct agatttagtt ttatgtgaac tcgcaaagaa
     2641 aggctataac aaagtaataa aaatagacat taccatagga gatttatcgg ggattgtaag
     2701 ggaacatata aaatactatt ttgaactttt aagccgggcc actccagctg caggtgcaac
     2761 tttgaccatc aactacaaaa aaagtaaatt tcgatgtgaa agttgtaaga tcatctacga
     2821 aagttgtgat ttttcattcc tatgtcccgt atgtaaaagc agaggaactt tggttgaaag
     2881 ctgtgcatcc atttatatta atagcataga ggtggaatag tgaaaatcaa agtcttaaaa
     2941 gatgtgcttg aggctaacaa ttacatagcg cgcaagataa gggaagaact tgcaaagaga
     3001 caagctatga tgattaacat aatcgggtct ccgggagcgg gaaagaccag ctttatcata
     3061 aaaaccatcg aagttcttga cgttaaagct gtagtgatag aaggggatgt gacttccgat
     3121 attgactcta gaaaagttgc cagtttcggt attccggtag tacaagtaaa cacaggcggg
     3181 ggatgtcact taaacgccaa tatgatagat ttggctctta aaaaaatccc cgtagaaaac
     3241 gccgtgatct ttgtagaaaa tgtgggcaat ttaatctgcc ctgcttccta cgatattgga
     3301 gaggatttta agcttgcaat atgcagtgtg ccggaaggcc atgacaagcc ctttaaatat
     3361 ccggccattt ttgaaaaggc cggggccatt gtattgaata aagtcgattt gataaagcat
     3421 gtagattttg acagggacac cttttacgag ggtgttaaga ccttaaaccc aaaagtaagt
     3481 gttttcgaag tttcttccat aaatggagat ggaattgagg agtggggcca gtggctcaag
     3541 tcgcagttca tggaaaaaat caatcgctga tttgttatca agctcaagat gaagttttgc
     3601 atgattatga ggctatatgc aaagcagttg atatgataaa gcaggggaaa atcattgccg
     3661 taaaaggcat agggggttat catctggctg tagatgcccg aaatgaggca gctgtatcga
     3721 gactcaggca aaaaaagcac aggtatggca agcctctggc tgtgatgatg ttaaatacaa
     3781 ctgaagtgga aaagtactgt gagatcagca tttctgaacg acaaatcctt gaaagtcaag
     3841 ctgcaccgat agtcctttta aagcgaagaa aaggcggtgc ccctttagca tatagtctca
     3901 caaagggcct tgacacttta ggagttatgc tgccctatac gccggtacat aaaacattaa
     3961 tggaaaagct cggcttcccg ctggttataa ccagcgggaa tatcagcgat gaacccattt
     4021 gcatatgtga agaggaggcc catagcagat taaagaatgt tgcagatggt tttttgcatc
     4081 atgacagacc cattttaaac aggatagatg actccgtatg cttttttgct gcagctggag
     4141 ttaggatggt cagaagaagt cgaggatttg caaatgcccc cattatgttg gataccccat
     4201 caaagcccgt ccttgcttgc ggagcctttt ataaaaatac cttttgtctt tttgatgata
     4261 ataaagccta tgtaagccat cacataggcg acctggacgg cagcctggct tattgctatt
     4321 ttaaggaaga aattcaaaaa tacaagagga aatataacat aaatccgagc ttagtagtaa
     4381 gggaccttca cccgtcatat ctttcaagtc tttatgccga tgaactcggc ctgccctgcc
     4441 ttaaagtcca gcatcatcat gcccatatag cgtccgtcat ggctgaatac ggtttaaggg
     4501 aaaaagtttt gggcatcgct tatgatggca caggattggg cagtgacgga aaaatctggg
     4561 gagcggaatt tttgctggct gaccttaagg atttccaaag gcttggccac ctaaaatacg
     4621 tcccccttcc aggtggtgac ctagctgtaa aacacccctt tcgaaccgcc ttaggcttta
     4681 tctatccgaa tatgcaccgg ttttccaaat acattgagag gctggatggc gagcaggtaa
     4741 aaatcataat aaatcagata gaaaggaagg taaacgctcc actcgcttcc agcatggggc
     4801 ggctttttga tgcggtagct tcgataatta acctaaggga cacagtagat tatgaaggcc
     4861 aggccgccct ggaacttgaa tccctcatct tgcccacaaa tgattgttac ggctaccgta
     4921 ttgaaagaga aggcacaggg tttttcattg atgtggatgt gctcctggag gaactgtaca
     4981 ctgactacat aaatggcgtt cctcaaggca tcatttctgc aaaatttcac aacaccgtca
     5041 taaattttac cctagacctg gcaaaaagaa taagagacat gaataatgta ggaaaagttg
     5101 tcctctccgg aggatgtttt caaaaccggt atctcctgga agggttaaat agggagcttc
     5161 aaggggaagg atttgcggta tacatcccca gtaaaatacc cgttaatgac ggaggtattt
     5221 cactgggcca ggctgccatc gcttcgacat acttttcgta aaaagaggtg aagtccaatg
     5281 tgccttgcag ttcccatgaa ggtcaaaaat attaggtggc cgaacgcaga agttttagct
     5341 gaaggtctac gccgggaagt caatatacag cttgtcccaa gagttaacat aggtgattat
     5401 gtcctggttc atgcaggctt tgcgatacag gtaattgacg gtaaatgggc aaaggagaat
     5461 ttagagctat gggaacaaat caagtcttga gaagtgtaaa taccgagctc aaaatcatgg
     5521 aagtctgcgg gacccattcg gaagttgttt caaaatatgc cctaaggcag ttttacacag
     5581 gcagggttaa actcgtttcg ggaccgggct gtcccgtttg cgtaatagct tccgaagaca
     5641 tcgatttaat cattgaatac gcaagatgcg gttttaccat tgttacattt ggggacttga
     5701 taaggattcc aggaaccgtg tccagcttag ctgtagagct tgcccatggt aaaagcatta
     5761 aagtcgttta ttcgccctta gatgcccttg ccatggcaaa ggacaatcct tcaaatcagg
     5821 tgatttttgt agctgtaggc tttgaaacaa ccgcccccct tgttgcactg acaatcatga
     5881 aggctaagaa ggaaggcata aaaaacttta gtgtcctaag ccttcacagg accataccgc
     5941 aaatacttag aactcttttt caaaccggtg taaatttgga tggtttgata ctgcctggcc
     6001 atgtgtgcac cattaccggc tcaaagccct ttgaattttt agcccgggac tttggcatta
     6061 ctgctgttat ttccgggttt gctcctgaag atataatgga aagtatcgag ataattagag
     6121 agaatttaca tcggccttca atatatattc agtacaaaag agccgtttca acttccggaa
     6181 atatggttgc ccaggaagca ataaaaaagg tttttaagcc ctgtgacgcc ttgtggaggg
     6241 cttttggcct tattaaagat tccggtcttg caataaaaga agcttggagc tttttcgatg
     6301 cccgcaagag atggcccata aagccaattc accataagca ggctttaaat aaaaaatgca
     6361 gctgtaaaga tatcatgaaa gggatcctga cgcctttaga gtgtcccctg ttcaggacat
     6421 tttgcagacc ggaaagcccc atcgggccat gtatggtgtc ttccgaggga agctgtgcca
     6481 tctactacaa gtacggaggg taatgtatgg acatagtttt aagcgctcac ggcgggggtg
     6541 gtaaactcac cgaagagctg atacaggaag tttttttaaa tgaatttgac agtgacgtat
     6601 taaatgaaat ggaagactct gccgttttag acatcgattt taataaactt gcctttacta
     6661 ctgacagcta cacagtaaaa cctctatttt tccccggagg cgatatcggc aggcttgccg
     6721 tatgcgggac gatcaacgac cttttgacga ggggtgctgt gcctttattt ttaagcgcct
     6781 cttttattat tgaagaaggc tttagactct atgatttaaa gagaatcgca agttccatgg
     6841 ccaggacctg ccgtgaggcc ggggtgaaaa tcgttgcggg cgacacaaag gttgtggcaa
     6901 tgggagatgc cgacgggatt tttatcaata cttcaggggc aggctatgtg ccttttggtg
     6961 tcaatatatc cgtaaaaaac gcaaagcccg gtgacaaggt cattatttcc ggcactgtag
     7021 gagaccatgg aatggcggtt ttatcaagca gagaggattt tacttttgac ccccctttgg
     7081 aaagtgactg tgcaccacta gcagacatag taaatacatt gcgaagtttt ggtgaatcag
     7141 tgagagtact ccgcgatcct acccgggccg gagtggcgga agtcctctac aacatatcac
     7201 gggccagcgg tgtgggtata gaaatctatg aagaaaagct gcccgtaaag cctcaggtgg
     7261 catctgcctg caacatgctg ggcctagact ttctgcagct ggccaatgag gggaagctcg
     7321 tggcagtagt agaaagcagt gcggcagtaa agatactgga agccataaaa aaatgtaaaa
     7381 atggccgtga agcggccata atcggaacag tcaacgattc cggccagata acggccagga
     7441 cggcactggg cacttcaagg attttaagcc gccctgtagg tgagctcgta cctcgaatat
     7501 gctaaaagca gttgacaaaa ttatgtagta gtaatacaat taaaacagag atacccccat
     7561 ggggtatata aagtgcggag gaggcgaaac aatggcggaa aatatagaaa aagccagtgt
     7621 taaaataaag ggaatgacct gtgctgcctg tgcggcacga attcaaaagg tcgtagggaa
     7681 aatgcccggt gttcaagatg ccagtgtaaa ctttgctacg gaaaaattga acgtgacatt
     7741 tgaacctgaa aaggtttccg tcgaggattt tataaaaaag gtaagagacg cgggctatga
     7801 tgtggttatg gacaaggctg agctcggctt aaaaggcatg agctgtgctg cctgtgcagc
     7861 ccgaattgaa aaagcccttt ccaaagcacc cggggtctat aaagcaacag ttaattttgc
     7921 aacggaaaca gcaacggttg agtttaacag cagcgagacc agcttgaaaa accttattaa
     7981 agtcgtaagg gatgcaggct atgatgcctt tgaaaagacc gatgtaaata tagacaggga
     8041 aaaggaagag cgtgaaaggg aaataaaaaa tctcggcagc cttgcggtga tttctgctat
     8101 actttcagct ccgctgctgt ttggtatgtt tttacacatg atgggaatgt caggaggaat
     8161 actgttcaat ccctggtttc agctggcagt atctgcaccc gtgcagttca taatcggttt
     8221 taggtactac aagggtgcct atcatgcctt aaagggcggc ggcgccaaca tggacgtatt
     8281 ggtggccatg ggaacctccg cagccttttt ctacagctta tacaatgcct ttactgtacc
     8341 cgctgaacag attcaccatt atttatattt tgaagcttca gcagttgtca taactctgat
     8401 tacctttggc aaatatttgg aagcagtcgc aaaaggcagg acttccgaag ctataaagaa
     8461 gcttatgggc ttggccccta aaaccgcaag ggtaattcgt ggcagcgagg aaatagacat
     8521 cccaatagaa gaagtggaag tgggagacat agttgtcgtg cgaccgggag aaaagatacc
     8581 agtagatgga gttatcaccg agggttattc ctccgtagat gagtccatgc tgaccggtga
     8641 aagcatacct gttgaaaaac acgtggggga tgaggtagta ggtgctacca tcaataagac
     8701 cgggaccttt aaatttaagg cgaccaaggt tggcaaggat accgtccttt cacaaatcat
     8761 taaattggtg gaagaagccc aaggttccaa ggcccccata caaaagctgg cagaccaaat
     8821 atcgggcgtc ttcgtccccg ttgtagtagt tgttgcggcc atta
//
LOCUS       DAONUO010000041         8825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6069_length_8825_cov_3.901596, whole genome shotgun sequence.
ACCESSION   DAONUO010000041 DAONUO010000000
VERSION     DAONUO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6069_length_8825_cov_3.901596"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /gene="aksA"
                     /locus_tag="PK768_02420"
     CDS             complement(<1..255)
                     /gene="aksA"
                     /locus_tag="PK768_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777287.1"
                     /note="in Methanococcus jannaschii this protein catalyzes
                     the condensation of alpha-ketoglutarate and acetyl-CoA to
                     form trans-homoaconitate; functions in alphaketosuberate
                     synthesis which is a precursor in coenzyme B and biotin
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitate hydratase"
                     /protein_id="HQA59856.1"
                     /translation="MKTGKKKIYLVDTTLRDGEQTAGVVFANREKIQIARMLDEVGVD
                     EIEAGIPVMGGDEKETVAAIAKLGLKARIMAWNRAVISDIQ"
     gene            complement(545..799)
                     /locus_tag="PK768_02425"
     CDS             complement(545..799)
                     /locus_tag="PK768_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59857.1"
                     /translation="MTFKYKQGIALTLLLFLSGSVTVYLGFSKGHDIAMVLSRPRGAS
                     GWITSGEMILACTYTPVIIGVSLIILSLVFSTFLFIKWIE"
     gene            complement(789..1025)
                     /locus_tag="PK768_02430"
     CDS             complement(789..1025)
                     /locus_tag="PK768_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59858.1"
                     /translation="MKDFLATFLIVLIFCYFFLFFGGVIVLENIFAGLVFVALVISIL
                     ITLYIRQETKIEELEARIKNLETKSEAKEANGDF"
     gene            complement(1048..1749)
                     /locus_tag="PK768_02435"
     CDS             complement(1048..1749)
                     /locus_tag="PK768_02435"
                     /inference="COORDINATES: protein motif:HMM:NF023031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59859.1"
                     /translation="MEYRYGKNINYEDFSSGRVLYHVKGMTNFPVRLAQEIYRRCLEH
                     SPKKNDIALYDCCCGGGYLLTVLGFLNQDTIRKIIGSDIDMEMLNVAEKNLSLLHEEG
                     INKRIAEIEQMIASFQKQSHIEARKSAIKLKSMIRSSIEFKLFHADALKEINLGEKPD
                     IIITDLPYGNLVDWNSDEENVIDKLLDSLHGICSSDTIIGICMDKKTKVRNEKFIRLE
                     RQKIGKRKFEILRKR"
     gene            complement(2064..2963)
                     /locus_tag="PK768_02440"
     CDS             complement(2064..2963)
                     /locus_tag="PK768_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HQA59860.1"
                     /translation="MTYKVSSDNYMFSFSKELEPVLRVKSGDVVEIETMDCFANQIKT
                     NEDKMESLDWNRINPATGPVYVEGTEAGDALKVTIEKIEIGDKGVVATGKGLGVLGHL
                     MEGLYSRVLDIKDGQVIFNEDISFPTNTMIGVIGVAPKEGSINCGTPGSHGGNMDTKL
                     IREGSVLYLPVFVEGALFGLGDLHASMGDGEIGVSGVEVCGSVTVKLEVVKNIGIANP
                     IVKTNDITATIASAITLDEAVTIATQDMANLIKLHSSMAMEEIATLMSAVGNLEISQV
                     VDPEKTARFAMPNEILERLNIQL"
     gene            complement(3170..4723)
                     /locus_tag="PK768_02445"
     CDS             complement(3170..4723)
                     /locus_tag="PK768_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HQA59861.1"
                     /translation="MSKKNIEEQLSDYIDALNDEREPEVEGAENDRELGKLIETVHRV
                     RTLREPSLPDGAYPERLARAVADEIQREKQRAIRKPTKSPSKSILRMLLPMTAVAACL
                     LLLVFVNSLGIFKQDVVYAMEKAVAKLNNYHGILEMRTRNAAGEEWLVRWVELWCEGD
                     KYALKQDDGTLTVNNGQQKWQINHEEREVAILPLLPDPTGSSFDLRDEAKRAKQYPHT
                     VVGTELVAGREAIKLAISPPGGEEYYLWVDAETNLPIQLETAMQKALQTTYTFVSFEP
                     NIEIPADIFAYRVPEGYKVVEDDPGQLVASVEEAAAISGFMPLLPKQAPVRILAFQDR
                     IVLDYGDTTIVESRATGEFKPEPNASIGSAADGPLEIWYERLRWRREGLEIQVEGARS
                     LELAGEIAADLSVPDTNQDITSKAKVKVPVDMGIVEANQKQVDSGSSPWQLDPLHVAL
                     TFVNLQVTPEGIQGEPQIDMPSFRLATNNSEEAVVEVDEGPIERVYLKRLVRQDETGI
                     WTVVGYDPR"
     gene            complement(4704..5285)
                     /locus_tag="PK768_02450"
     CDS             complement(4704..5285)
                     /locus_tag="PK768_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQA59862.1"
                     /translation="MIAVDLYKKAIDGDPDAIEKICLDTWQPLYRFIYYKVQNREEAE
                     DITQETYVKTLKYLQKSTIPLEKFLAFMKTVSLNVIRDRWRQKKRGWVSVNFREINPA
                     EAAYVDKQNDIAQRLVLENALAELSQEQRAIIDLRIIKGYSVAETAKLLGKTEGAVRT
                     AQYRALKALARILDEAAAKDRTRRREQLEQKEY"
     gene            complement(5448..5975)
                     /locus_tag="PK768_02455"
     CDS             complement(5448..5975)
                     /locus_tag="PK768_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59863.1"
                     /translation="MIKVRSMKKQLKYIFLFLIFICLLTGCFSQALYKKISATLGIKI
                     PANVKIEYEDTHGGFHGDGESFAKIVFDPYQAEEILRQIESLDSWHELPLSENLHLIM
                     YGGEKDNVEYAYNFAKSFGIPHVKNGYWFFVDRHDKSSSPEKDENIFKRSSFNFTLAI
                     YDIDTNTLYFFELDT"
     gene            complement(6242..6454)
                     /locus_tag="PK768_02460"
     CDS             complement(6242..6454)
                     /locus_tag="PK768_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA59864.1"
                     /translation="MPIIVNLDVMLAKRKMSVTELSEKVGITMSNISILKNGKAKAIR
                     FSTLEKICEVLDCQPGDILEYAKEEK"
     gene            complement(6465..6944)
                     /locus_tag="PK768_02465"
     CDS             complement(6465..6944)
                     /locus_tag="PK768_02465"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HQA59865.1"
                     /translation="MERKELTKWLKIVIVFTGLIGLFLCFIVAPFLGLEMKFMYPEYG
                     YMFWPCLIFIWATGVPFYIALYKGWLICGEIANDNSFCEENVQRLKEISKLALLECIL
                     YLAAMIILLALNLLHPSILLMILFIIFVGISIAVMAATISHLVEKARELKEENDLTI"
     gene            7197..7931
                     /locus_tag="PK768_02470"
     CDS             7197..7931
                     /locus_tag="PK768_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HQA59866.1"
                     /translation="MGNVAAFFDIDGTLFRNSLMIEHFKKMIRYEVIDPSLWYGRLRD
                     SYSEWDKRYGDFNDYMDELVQVYVKELKGINKSYIEFIASQVININWDKVYRYTRARI
                     NWHKEQGHMIFFISGAPDFLVEKMAEKYRVTAYRATRYIVDENNNFTGQVVSMWDSEN
                     KQKAVNELVAQYKVDLTASFSYGDTNGDLSMLKIVGNPVAINPVKKLLMAIKEDKELF
                     EKTTIIVERKDLIYKLSPSVELIQFE"
     gene            complement(7949..8806)
                     /locus_tag="PK768_02475"
     CDS             complement(7949..8806)
                     /locus_tag="PK768_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA59867.1"
                     /translation="MSGMLMMNRPILVALAAIFTISVFCTWQNNAITTTQIDYISPKI
                     PEEFNGYRIVHISDLHNKQFGKNQERLLKRIRAVSPDIIVITGDLIDRRKYDLETAML
                     FINGAIKIAPLYYVSGNHEAWSGDYANISERLRSSGVQILDDTKATMIRGKGKIDILG
                     LSDPDFLTYSYLEGTNTSKLREHLERLSDDAVFQILLCHRPELFEIYARENIDLIFSG
                     HAHGGQVRIPFVGAIVAPDQGFFPKYSSGAYTQNQSTMIVSRGLGNSIIPIRIFNRPE
                     IVVVTLRSE"
BASE COUNT         2406 a         2067 c         1597 g         2755 t
ORIGIN      
        1 ctgtatgtct gaaattaccg ccctgttcca ggccattatc ctggccttta agcccagctt
       61 ggctatagca gcaacggttt ccttttcatc tcctcccata acgggaatcc cggcctcgat
      121 ctcatctact cctacctcat ccagcatccg ggctatctgg attttttcac gattggcaaa
      181 gactacaccg gcagtctgct ctccgtctct taaagtggta tccacaaggt aaattttttt
      241 ctttcccgtt ttcatagcag ctcctcctct tgcttacttg aataattgta tacaattata
      301 caggtgtaat tggatttcgt caagagcttt tttggaaatt tttctataga atttattgta
      361 atgttttcgc ccatatttta tagttgtcac aaatgatatc tcttaatatt tataagtccc
      421 gcttgaaact cggtctgcaa aagctggtac aaacaaaaaa aggacggtct ccgtggtcac
      481 cggtcacccc tttctcccgc gaccacgaga accgtcccta tcatttttca ctcttaaaat
      541 gcttttattc aatccacttt ataaatagaa acgttgagaa aactaaagac aagataatta
      601 aagacacccc tattattacc ggcgtatagg tacaagctaa aatcatttct ccagatgtta
      661 tccaaccgct tgctcctctt ggccttgaaa ggaccattgc aatatcatgg cccttggaaa
      721 aacctaaata taccgttaca ctgccgctta aaaacagtaa taaagttaat gcaataccct
      781 gtttatattt aaaagtcacc atttgcctcc tttgcttcgc ttttagtttc gaggttttta
      841 atccgcgctt ctaactcctc aattttagtt tcctggcgta tatacagcgt tattaaaata
      901 gaaataacga gtgcaacaaa aacgaggcct gcaaaaatgt tctctaaaac aatcaccccg
      961 ccaaagaata gaaagaaata acaaaatatg agaactatta aaaaggtagc aagaaagtct
     1021 ttcattgcct atccccctaa gtagtaatta tctttttctc aagatctcaa attttctctt
     1081 tccgattttt tgtctttcca atcttataaa tttttcattc ctaacttttg tcttcttgtc
     1141 catgcaaata cctatgatgg tatccgaaga acaaattcca tggaggctgt ctaacaactt
     1201 atctatgaca ttctcttcat cggaattcca atctaccagg ttgccatagg gaagatccgt
     1261 tatgataata tcaggttttt ccccaagatt tatctccttt aatgcatctg catgaaaaag
     1321 cttaaattcg atactgcttc taatcatact cttaagtttt attgcgcttt ttctagcttc
     1381 aatatgtgat tgcttttgaa aactagctat catctgctct atctccgcta tgcgcttatt
     1441 tatcccttct tcgtgcagca atgataagtt cttttctgca acattcagca tttccatatc
     1501 aatatcgctt cctatgattt ttctaatagt gtcctgattt aaaaaaccca aaacggtaag
     1561 caagtatccc ccgccgcagc agcaatcata caaggctata tcattctttt taggagaatg
     1621 ctctaagcac cttctgtata tctcctgggc cagtctaaca ggaaaattcg tcatcccctt
     1681 tacatgatac aggactctgc cgcttgaaaa atcttcgtaa ttgatatttt tgccgtacct
     1741 gtattccata caaaaacccc atttatatca ggtaatcaat acttattata ccaatagaaa
     1801 agaattagag aaagctggcc gctaaaatat aaatggagca cagtattgaa atttctttac
     1861 agagaagcta agcggattcc cccgataaat gtcacatgct ggaaaaagga cataaaaaac
     1921 tggaccccat tattaggagt ccatgtacac ttttttttta gcagtctact gacaaagagc
     1981 agttcattct tggaagcttt ttatatgcac tttttgttct attaggctag ttttgtcttg
     2041 gtattataag ctttgctgaa tatctataat tgtatgttta gtctttccag gatttcattt
     2101 ggcatggcaa aacgggcggt tttttccgga tctacgacct gtgagatttc taaattgccc
     2161 accgcgctca tcaaagtggc tatctcttcc atggccattg agctatgtag ttttatcaga
     2221 ttggccatat cttgggtggc tatggtgaca gcttcgtcta aggttatggc cgatgctatc
     2281 gttgccgtta tatcattggt ttttacgatg ggatttgcaa ttcctatgtt ttttaccact
     2341 tctaacttaa cggttaccga gccgcacact tccactcctg atacccctat ttctccatct
     2401 cccatggagg catgtaaatc acctaatcca aacagggccc cctccacaaa tacgggcagg
     2461 tacagcaccg aaccttccct gattagtttt gtgtccatgt ttccaccatg ggatcccggt
     2521 gttccgcagt ttatgctgcc ctctttaggt gcaacaccaa ttacaccgat catcgtgtta
     2581 gtggggaaag agatatcttc gttgaaaata acctgtccat cttttatatc aaggactctc
     2641 gagtaaaggc cttccataag atggcccagc acccctaaac ctttgcccgt ggccacgacc
     2701 cctttatcgc ctatctctat tttctcaatg gtcactttca gagcatctcc ggcctctgta
     2761 ccttcgacgt aaaccggacc cgttgcgggg tttatcctgt tccagtcaag ggattccatc
     2821 ttgtcttcat ttgtcttgat ttgatttgcg aaacagtcca tggtttcaat ttcaactaca
     2881 tcacctgatt tgacacgaag aacgggttct agctcttttg aaaagctgaa catgtagttg
     2941 tcgctcgaaa ctttataagt cacttaatct tccacctccc ggattttaaa taattttgtc
     3001 ttttttctat tatacccctt ttccagtttt ttgtgaattt aattcatctt tgccatttat
     3061 ttccaataat agtaagtttt aacgcggatc atattttaaa tatctgattc gcatgaaaaa
     3121 gggccttgtc tcttttccaa aagacaaagc ccgcaaatag tattaaattt tagcggggat
     3181 catatcccac taccgtccaa atacctgttt catcctgtcg gacaagcctc tttaggtata
     3241 cccgctcgat ggggccttca tctacttcta cgaccgcttc ttcggaattg tttgttgcca
     3301 gcctaaatga aggcatgtct atctgtggtt ccccctgaat tccttcggga gttacctgta
     3361 aattgacgaa agttagggcc acatgcaacg ggtcaagttg ccaggggctg ctgccgctgt
     3421 ctacctgttt ttgattggcc tcgacaattc ccatgtctac cgggaccttg accttagcct
     3481 tgcttgtaat atcctggttt gtatctggga cgctaaggtc tgccgcaatc tctccggcca
     3541 gttccaagct tcttgctcct tcaacctgga tctcaagtcc ttcccgcctc cacctcagcc
     3601 gctcatacca aatttcaagg ggcccatctg ccgcgctgcc tatggaagca ttgggttcag
     3661 gtttaaactc accggtagct cttgattcca caattgtagt atctccgtaa tccaggacga
     3721 ttctatcttg aaatgccaag atgcgaaccg gcgcctgttt cggaagtagg ggcataaatc
     3781 cgctgatagc agctgcctct tcaacgctag ccaccagctg gccggggtca tcttcaacta
     3841 ccttatatcc ttccggcacc ctgtaagcaa atatatcggc aggaatctcg atattaggct
     3901 caaaactaac aaaggtatac gtagtttgca gtgccttttg catagccgtt tcgagttgaa
     3961 tgggcaaatt ggtttccgca tccacccata aatagtattc ctctcctccc ggtggagaaa
     4021 tggcaagttt tattgcctca cgtccggcaa ctagctctgt gccaactacg gtgtgaggat
     4081 attgctttgc ccgctttgcc tcatcccgca agtcaaagct gcttcccgtc gggtccggca
     4141 gcagcggtag gatggccact tccctctctt cgtggttaat ctgccacttt tgctgtccgt
     4201 tatttacggt cagagtgccg tcatcttgct ttaaggcata tttatcgccc tcgcaccaaa
     4261 gctccaccca gcgcaccagc cattcctcac ctgccgcatt ccttgtccgc atctccagga
     4321 tgccgtgata gttattgagt ttggccaccg ccttctccat ggcataaacc acatcctgct
     4381 tgaaaatgcc cagggaattt acaaagacca acagcagcag acaagccgcc acagccgtca
     4441 tgggcagcaa catccttaga atggatttgg atggagattt agtgggtttt ctaatggccc
     4501 tttgtttttc cctttggatt tcatccgcta cggcccgggc cagtctctcg ggataagccc
     4561 catcaggcaa gctaggctcc cttaaagtgc ggacccggtg aaccgtttct atcagctttc
     4621 ccaattcccg gtcgttctct gcgccttcaa cttcgggctc gcgctcgtca tttaaggcat
     4681 ctatataatc ggaaagttgt tcttcaatat tctttttgct caagctgttc cctcctccta
     4741 gttctgtctt tagctgccgc ttcatccaaa atcctggcca aggctttcaa tgcccgatat
     4801 tgggccgttc ggaccgctcc ttcagtcttc cccagcagct ttgccgtttc tgccacagaa
     4861 tagcccttga taattcgcaa atcgataatt gcccgctgtt cctggctgag ctctgccaag
     4921 gcattttcca atacaagtcg ctgggcaata tcattttgtt tatcgacata ggctgcctct
     4981 gccggattta tttcgcggaa gttgaccgat acccatccgc gcttcttttg ccgccacctg
     5041 tcacgtatca cgttgaggga taccgtcttc ataaaagcca gaaatttttc aagcggaatt
     5101 gtactttttt gaaggtactt taaggtctta acatatgtct cctgggtgat atcctctgcc
     5161 tcctcccggt tttgcacttt gtagtagatg aaacggtaaa ggggctgcca agtatcaaga
     5221 catatcttct ctattgcatc cggatcacca tctattgcct ttttatacag atcaacagct
     5281 atcacaagtt ctacaccttc tttcttcccg gccggttttt gtcttccata aataaagacg
     5341 aaactgacac ccaaaatgtc acacatgaat atgaataatt agggacgggt acaaaaaatt
     5401 cactcgtgaa ttttttgtac ccgtccctaa ttattcatcc atcaccatta tgtatcaagt
     5461 tcaaaaaaat atagtgtatt tgtatcaata tcgtatatgg caagggtaaa gttaaaggaa
     5521 cttcgcttaa aaatgttttc atctttttct ggggagctgc tcttatcatg tcggtcaaca
     5581 aaaaaccaat aaccgttttt aacatgagga attccaaagc ttttggcaaa attataggca
     5641 tactccacat tgtccttttc tccgccatac atgataagat gaagattttc gctcaggggt
     5701 agctcatgcc aactatcaag actttctatt tgccttaaaa tttcttcagc ctgatatggg
     5761 tcaaatacga tttttgcaaa gctttctcca tccccgtgaa atcccccatg agtgtcttca
     5821 tattctattt ttacatttgc aggtatttta attcccaagg tggctgagat ttttttgtat
     5881 aaagcttgtg aaaaacatcc tgttaataag cagataaaaa tcaaaaataa aaatatgtat
     5941 tttagctgtt ttttcatgga tcgcacttta atcattttaa gcctgagaat ctgtcacctc
     6001 tcctttaaag ccacggaaat tgcaatcaaa attactccca ttataaataa acctaaatac
     6061 ataaataact gaaaaggccc tcccccgaaa tggctaaatg ggtacggtcc gtttataatc
     6121 catatatacc tttcataact gccatgaata aaccaaaaca gtaccgaagg gctaattatt
     6181 aaaatgaatc ctattcccaa aaacaaacct gatatatact cttttacggt tctccccatt
     6241 tttacttttc ctcttttgcg tattctaaga tatcaccagg ctggcaatct aatacttcac
     6301 atattttttc tagagttgaa aatcgtatgg cctttgcttt tccattcttc aatatagata
     6361 tatttgacat tgttatcccc accttctcag atagctcagt aacactcatt ttccgtttag
     6421 ctaacataac gtctaaattt actattatag gcatcacatc accctcatat tgtcaaatcg
     6481 ttttcttctt ttagttccct tgctttttcc accagatgag aaatggttgc agccataaca
     6541 gcaattgaaa ttccgacgaa aattatgaat aatatcataa gtaatatgct aggatgcagc
     6601 aaatttaaag caaggagaat tatcatggcc gccaagtaaa gtatacattc cagcaaagcc
     6661 aacttgctta tctcctttaa cctctggaca ttttcttcac aaaatgaatt gtcatttgca
     6721 atttccccac atatgagcca gcccttgtaa agggcaatgt aaaaaggaac tccagtagcc
     6781 caaataaaaa tcagacaagg ccaaaacata taaccatatt ctggatacat aaacttcatc
     6841 tcaaggccta aaaagggagc aacaataaag cataagaaca gacctattag accagtaaaa
     6901 acaataacaa ttttaagcca tttggttaat tcctttcgtt ccatttcatc atctccttaa
     6961 tcaacattat aattgatttt tgtgtgtatt gcaatacatt tttattaaga tacgattaat
     7021 atttatcgtt tttcgatatg gatacttaag tgaataatta tggttgggta caaaaaaaat
     7081 catcccgatg aatttcttgt gcctgtccct aattattcat tgatatcttt tttgttttaa
     7141 atgatataat atacaataat gctactcctt aataaatatt ggaggaggcg aaagctatgg
     7201 gaaatgttgc cgctttcttt gacattgacg gcaccctctt tagaaattcc ctcatgatcg
     7261 agcattttaa aaagatgata aggtatgaag tgatagaccc tagcctctgg tacggccgcc
     7321 tgagggattc ttattctgaa tgggataagc gctacgggga ttttaatgat tacatggatg
     7381 aattggtgca ggtctacgtt aaagagctca agggcataaa caagtcctat atcgagttta
     7441 tagcaagtca ggtaataaat ataaactggg ataaggttta caggtatacc agggccagga
     7501 tcaactggca caaagagcag ggccacatga tatttttcat atccggagct cccgattttc
     7561 tggtggaaaa gatggcagag aaataccggg tcaccgccta cagagccacc cggtacatcg
     7621 tggatgaaaa caacaacttc accggtcaag ttgtcagcat gtgggattca gaaaacaaac
     7681 aaaaggctgt aaacgaattg gtggctcaat acaaagtgga tttgactgcc agcttctcct
     7741 acggggatac caacggtgat ctttctatgc ttaagatagt tgggaatccg gtggcaataa
     7801 atcccgtcaa gaagctttta atggccataa aagaagacaa ggaactcttc gaaaaaacca
     7861 ccataatcgt agagcgaaaa gacttgatat acaagctaag cccttccgta gaacttatcc
     7921 agtttgaata atctgggacg ggtacaaatt attcacttcg taaagttacg acaacaattt
     7981 caggcctgtt aaaaatcctg atggggatta tgctgttgcc caggcccctg cttacaatca
     8041 ttgtcgattg attctgtgtg taggcgccgc tgctatactt cggaaaaaag ccctgatccg
     8101 gcgccacaat ggcccctaca aaaggtatcc tgacctgccc accatgggca tggcctgaga
     8161 aaatcaggtc tatgttctcc cttgcatata tttcaaagag ctccggcctg tggcaaagca
     8221 ggatttggaa gacggcatca tccgataagc gctcaagatg ctccctcaat ttggaggtat
     8281 ttgtcccttc caaataacta tatgttaaaa aatccgggtc ggacaagccc agtatgtcta
     8341 ttttcccttt gcctctaatc atagttgcct ttgtgtcatc aagtatttgc acaccggagc
     8401 ttctgagtct ttcgctgata ttggcataat caccggacca ggcctcgtga tttccggaaa
     8461 catagtaaag gggagcgatc tttattgcgc catttatgaa aagcatggcc gtttccaagt
     8521 cgtattttct tctatcaatc agatccccgg taatcacaat tatgtccggg gacactgcgc
     8581 ggattctctt taataggcgc tcctgatttt tgccaaattg tttattgtgt agatctgaaa
     8641 tatggacaat cctgtatccg ttgaattctt caggtatttt aggactgatg taatctattt
     8701 gcgtggtagt tatggcattg ttctgccatg tacagaaaac tgaaatggta aaaattgcag
     8761 caagagcgac taatattggc ctattcatca ttagcatacc cgacattcct ttttttgatt
     8821 taatg
//
LOCUS       DAONUO010000042         8658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6240_length_8658_cov_3.737533, whole genome shotgun sequence.
ACCESSION   DAONUO010000042 DAONUO010000000
VERSION     DAONUO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6240_length_8658_cov_3.737533"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..546)
                     /locus_tag="PK768_02480"
     CDS             complement(169..546)
                     /locus_tag="PK768_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59868.1"
                     /translation="MQKTFFEKTGSGKHVIEAVVTRTCEGINIYIGGGEKSHIGAIVV
                     SQPRYSLKGDGTISCTTSVFNIVGHKDDIIAIPLAEKLCKKTGQVVVVCAGVHVDNAT
                     LEDIKKLEKNGEELTEKLLKKFD"
     gene            complement(552..1781)
                     /locus_tag="PK768_02485"
     CDS             complement(552..1781)
                     /locus_tag="PK768_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HQA59869.1"
                     /translation="MKAIVVDIDGDYLIVANSRGDFIRIYNDYPGCQVGDEITVRETK
                     AGFFGSMLSSFAKKKALVVAACFMLMLITSYGVYGYLNPVTYVTIDINPSVEFSLNRY
                     DLVRDVCALNEDGKIIIGDGREYKNMKFDKALNLLLSRAVEAEFLNEDANTVMLTVSS
                     VKDSTSSNKKQQLQEIAEKQLHKVIEREEESSSLRTEQATKGLATEETSAQGTNKRSI
                     KIIVENTTYEKHQEAKRMAISQGKLVLYEKLKRVKPDVALEHIKETPVGQILKELEKI
                     GPKDKPGPESRPSTNRDDKRQGNDKKEVMNHGQLHSEKIREQKDHRSEQMEKIQKEQK
                     NKIKDKIKEVNEKIKDAKDQLKEALKETREKGKDLKEEKARKKENTNEKGKQKEKSND
                     NRKSKQWDKESKYRRKD"
     gene            complement(1787..2497)
                     /gene="sigI"
                     /locus_tag="PK768_02490"
     CDS             complement(1787..2497)
                     /gene="sigI"
                     /locus_tag="PK768_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigI"
                     /protein_id="HQA59870.1"
                     /translation="MEQHQSLNERVMNIKHDTEEINRLIEEYKPFIASTVEQHLARYV
                     EYGIDDELSIALMAFHEAIEKYDIKKGNFLSFAKMTIKNRLIDHYRKEQKIMGPVVYV
                     EQAKEQDDEELLNLYVDESIKEYTEAQKSELRKLELQEIKKELAKWGLSFADVAKSSP
                     KQEGTRRTYLAAIDFIMKSPELIDIIKNKKYLPVEKIAAGLKIPRKRIERGRNYIIAA
                     VLILSGDYQFINEYIKWR"
     gene            complement(2703..3974)
                     /locus_tag="PK768_02495"
     CDS             complement(2703..3974)
                     /locus_tag="PK768_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HQA59871.1"
                     /translation="MQLFLERVAIPINSSKVYDENKVVSQAIRPGDYLPFGQLLILGL
                     QHAFTMFGATVLVPILTGISVSVALFTAGIGTLWFHLVTNRKVPIFLGSSFAFIAPVA
                     LAVKEMGIPAAQGGIIVAGLLYCAMAAFIHFLGPKFMESLLPPIVTGPIIMVIGLNLA
                     PTAIESASENWFIAIAVLMVVAYVNLYAKGFLRMLPVISGIIVGYILCLFANLVDFTP
                     IREAAWFAVPDFSLPVFNLSAIGLIAPVAIASMVEHVGDILSVGATVEEDFVKDPGLP
                     RTLIGDGIATTLAGLLGGPANTTYSENTGVLALTRVWKPEVMRVAAVIAIGLSFVQKL
                     GAAIRTIPAPVIGGISIVLFGMIASVGVRTVVENQIDFKQPRNLFISSVILVVGIGGA
                     VINFGGGLQIGGMGLAAILGIILNQVLPRES"
     gene            complement(4033..5175)
                     /gene="wecB"
                     /locus_tag="PK768_02500"
     CDS             complement(4033..5175)
                     /gene="wecB"
                     /locus_tag="PK768_02500"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQA59872.1"
                     /translation="MKILSIFGTRPEAIKMAPLVKELQQRDCFQCEVAVTAQHRQMLD
                     QVLSLFGINVDYDLNIMTDRQSLFSITTKALLGLRDVLDSSKPDMVLVHGDTTTTFAG
                     ALAAFYDGIKVGHVEAGLRTHNKWLPFPEEMNRKLTGALADLHFAPTRTARKNLLREG
                     VSSQNIFVTGNTVIDALRTTVKEDFVFSTDALNNLDFDRQKVVLVTAHRRENLGEPLE
                     NICHAIRKLADSFRDIKVVYPVHLNPAVRDTVFQILGQHERILLLDPLDTDEMHNLMA
                     KSFLILTDSGGLQEEAPSLGKPVLVLRHETERPEAVKAGTVKIVGTDREVIYKEAARL
                     LTDKQEYDRMANAVNPYGDGRASIRIADFLLYYFGLQEDKPREFCT"
     gene            complement(5198..6235)
                     /locus_tag="PK768_02505"
     CDS             complement(5198..6235)
                     /locus_tag="PK768_02505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQA59873.1"
                     /translation="MPRYIYGFLASMILAYLVTPSVMKLAWKIGAIDKPGDPRRIHKQ
                     PMPRLGGLAIYIAFVLTGLLTLPLKQGYIQGIFLGASLIVIVGIVDDIYNLPARVKLL
                     GQIAAAGILVAFGVKVEIITNPLGGMFVLGKLAIPATLFWVVGITNTLNFIDGLDGLA
                     AGIAAIAAFTMMLVNISLEQANGALVMAILAGAAIGFLPYNFNPAKIFMGDTGAMFLG
                     FVLAAQAVEGTVKSATAIALIVPILALGLPIFDTAFAILRRFINGKPLMQADKGHVHH
                     RLIAIGLSQKQAVLFMYIISISLGICSLVLARIGVKQAVIALSLVLFMLLVSVRYINI
                     ALDTKKSTHGM"
     gene            complement(6325..6744)
                     /locus_tag="PK768_02510"
     CDS             complement(6325..6744)
                     /locus_tag="PK768_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HQA59874.1"
                     /translation="MRKDWDQYFLDIAFQVRDRSTCPRLKVGAVVVKDNRIKGTGYNG
                     SPSKMAHCDDVGCMMRANHCVRTIHAEVNALLECSPEERKGATIYVTARPCAECSKLI
                     INSGITRVVYAQDYHVDYDWFKEAPWIEVVHLPREKP"
     gene            complement(6769..8175)
                     /locus_tag="PK768_02515"
     CDS             complement(6769..8175)
                     /locus_tag="PK768_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQA59875.1"
                     /translation="MKLFFSNTDKVMDAIINNRPIASQNPKVKQVFSKIGQLEDLKDD
                     AREMLLKTISLSASLGNIEVNIKHLMDEIETIMNKLGLQSENTLAFVQETTASMGEID
                     TAIEDNVKNIDEIHANIENIVKSNNKNMESLKLMGEVCKKVTESNSAVNQTLTKLLEH
                     LKEIGGIVEVIEEIADQTNLLALNASIEAARAGEAGRGFAVVSEEIRKLADSTKESLE
                     KFKSFTQEIHRDSAQSLESMERTNEVMAQIPEVTSAIREAVEGSFTAINKIKSDMEGF
                     VASFQQISSAVSEVSSAMHSLSTETEEVVYIVNTLESDIKRLESIKDQINYMDDGFIA
                     QNKEYYQKFMDKNNPVTKKELAGILENAKKQHSLWMDTLKEAVDEGKLIPLQVDSNRC
                     AFGHFYNALIIKDGSIHELWERIDEYHENLHNAGQQALIAIRDGDLEEAKNQYQIAKE
                     NSKHVFEILDRMIENLSQ"
     gene            complement(8338..>8658)
                     /gene="upp"
                     /locus_tag="PK768_02520"
     CDS             complement(8338..>8658)
                     /gene="upp"
                     /locus_tag="PK768_02520"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQA59876.1"
                     /translation="RDPETLMPVEYYCKLPSDVSERELIILDPMLATGGSAAKAVEVL
                     KDKGATSIKLMCLIAAPEGIEAVHKEHPDVEIYTAAIDERLNSKGYIVPGLGDAGDRL
                     YGTK"
BASE COUNT         2136 a         2116 c         1702 g         2704 t
ORIGIN      
        1 ttaaatatct tgttattttt gccgttcttc ttaaaaacgg ggtacgctta taagcttcgg
       61 aggtattgtc ctatattcta cattgtttga cgatatcctt tagaaactta aattctgtga
      121 gacttttgta catattcaag ctcccttcag gctgggcgag aaagtgaatc agtcaaactt
      181 ctttagtagc ttttcagtta actcctcccc atttttttct agctttttta tatcttctaa
      241 agtggcattg tcaacatgga caccagcgca tactacaacg acttgtccag tttttttgca
      301 cagcttttca gcaaggggta tggcgattat gtcatctttg tggccaacta tattaaaaac
      361 cgacgtggtg cagctaatcg tcccatctcc tttcaaactg taacgaggtt gactcacaac
      421 gatagcgcct atatgagatt tttcaccacc gcctatataa atgttgattc cctcacaggt
      481 tctagtgacc acggcttcta ttacatgctt tccagatccc gttttttcaa aaaaggtttt
      541 ttgcatttgt ctcaatcctt tcgacgatat ttgctctctt tatcccattg tttagatttc
      601 ctgttatcat tcgacttctc tttctgtttt cctttttcgt tcgtgttttc cttctttctt
      661 gctttttctt cttttaagtc tttgcctttc tctctagtct cttttaaggc ttcctttaat
      721 tggtccttcg catctttaat tttctcattt acttctttaa ttttatcctt tattttattc
      781 ttttgctcct tttgtatttt ctccatttgt tcacttctat ggtctttttg ctctcttatt
      841 ttttctgaat gcagttggcc atgattcata acttcttttt tgtcattgcc ctgtctttta
      901 tcatcgcggt tagtacttgg ccttgattcg gggccgggtt tgtcttttgg ccctattttt
      961 tctaattcct ttagtatctg gcccactggg gtctccttga tatgttccag ggcaacatca
     1021 ggcttgactc ttttcaattt ctcgtataac acaagttttc cctgggatat ggccatcctt
     1081 ttggcttcct ggtgcttctc atacgttgta ttctccacta tgatttttat acttctctta
     1141 tttgtacctt gggcggaagt ctcttcggtt gcaagcccct tcgtggcctg ctctgtccgc
     1201 aaagaactgc tctcctcttc cctttcaatc accttgtgca attgcttttc cgcaatttcc
     1261 tgcaactgct gttttttgtt agatgaagta gaatccttta cacttgatac ggtaagcatt
     1321 acagtattgg catcttcatt caaaaactca gcctcaacag cccttgaaag gagcaaattt
     1381 aatgccttat caaatttcat attcttatat tcacggccgt cccctatgat aatcttgccg
     1441 tcttcgttca gggcacaaac atcccttacc aggtcatacc tgtttaatga aaactcaaca
     1501 ctgggattga tgtctatcgt tacgtaggtt accggattta aatagccgta cacaccgtaa
     1561 gaagtgataa gcataagcat aaaacatgcc gcaacgacca aggccttctt tttggcgaaa
     1621 gatgataaca ttgagccgaa aaatcctgct ttggtttccc tgaccgttat ctcgtcgccc
     1681 acctggcagc cgggataatc attgtatatt cttatgaagt cgcccctgct atttgccaca
     1741 atcaaataat ctccgtcaat gtctacaact atggctttca atgttatcac ctccacttga
     1801 tgtactcgtt tataaattgg taatcccccg ataaaatcaa caccgctgct attatataat
     1861 ttcggcctct ttctattcgc ttacggggta ttttaaggcc tgcagcgatt ttttccaccg
     1921 gcaggtactt tttattttta attatatcaa ttaattcagg gctcttcata ataaaatcta
     1981 tagctgcaag gtacgtgcga cgagtgcctt cctgtttggg cgagcttttg gccacgtcgg
     2041 caaagctaag gccccacttt gcgagctcct tcttgatttc ttgaagttcc agcttcctca
     2101 gctcactttt ttgcgcctct gtatactcct ttatcgattc atccacgtag agattcaata
     2161 attcttcatc atcctgctct ttggcctgct ctacgtaaac taccggtccc attatttttt
     2221 gttcttttct atagtgatct atgagccggt tctttatggt catctttgca aaggataaaa
     2281 aattgccctt ttttatgtca tacttttcaa tggcttcgtg aaaggccatg agtgcaatgc
     2341 tcagttcatc gtcaatgccg tattccacgt acctggccag gtgttgttct accgttgatg
     2401 ctataaaggg cttgtactct tctatcaggc gatttatctc ttcggtatca tgttttatgt
     2461 tcattactct ctcatttaag ctttgatgtt gttccataaa atcccctaca tttccaaaat
     2521 ttgatgctac tccgactata tatcaattct acacagcgcc ataaattcct ttgacaacat
     2581 tatgcaaatt ctggaaatca tttattaaaa aaaagaggaa cgcacaagtt cctctttatt
     2641 gtaatacttg atatatacca agttcggtgc agggcaccat agtaatttac tctctacttt
     2701 gctcaacttt ctctcggcag cacttgattt aagattatgc caaggatggc tgccaagccc
     2761 ataccgccga tttgtaagcc gccaccgaaa tttatgacgg ctcctcctat acccactacg
     2821 agaattacgc tggagatgaa aaggtttcgg ggctgtttaa aatctatctg gttttccacc
     2881 acggtcctga ctccaacact ggcaatcatt ccaaaaagca ctatggatat gcctccgatt
     2941 acaggggccg gaatggtcct tatggctgca cctaattttt gcacaaaaga aaggcctatg
     3001 gcaattactg ccgcaaccct catgacttca ggtttccaaa cccgagtcag ggccaggact
     3061 cccgtattct ccgaataggt tgtgtttgca ggtccgccca gaaggccggc tagggtggtt
     3121 gcaatgccgt cgccaataag ggtcctagga aggcccggat ctttaacaaa gtcctcttcc
     3181 accgttgccc ccacagagag tatgtcccct acatgttcca ccatagaggc tattgccact
     3241 ggagctatta agcctatagc tgacaggtta aaaaccggca gtgaaaaatc cggcacggca
     3301 aaccaggcag cttccctgat gggggtaaaa tcgactaaat tcgcaaaaag acaaagtatg
     3361 taccctacaa taattccgga aataacgggc agcatcctca aaaaaccctt ggcgtacagg
     3421 tttacataag cgacgaccat gagcacggcg atggctatga accaattttc gctggcactt
     3481 tctatggcag taggcgctag gttcaagcct atgaccatga ttatagggcc ggtaactatg
     3541 ggaggcagca atgattccat gaatttgggt cccaagaaat gtataaatgc agccattgcg
     3601 caataaagta aacctgctac gataatgcca ccttgggccg ccggaattcc catttccttt
     3661 acggctagag ccaccggtgc aataaaggcg aaggatgaac ccaaaaatat aggaactttc
     3721 cggttcgtaa ccaaatgaaa ccagagagtt ccaataccag cagtaaataa agctacactg
     3781 acactgatac ctgtgaggat aggcaccagc accgttgcgc cgaacattgt aaaagcatgc
     3841 tgaaggccaa gaatcaatag ttgaccgaaa ggtaaataat ctcccggccg tatggcttgt
     3901 gaaactacct tgttttcatc gtagactttt gaagagttaa taggaatcgc caccctttcc
     3961 aaaaaaagtt gcaaaatatg cctcttatac tttagcggaa aatatgtcta aaatcaagct
     4021 attttgtcga gttcatgtac aaaattctcg tggcttgtcc tcttgcaggc caaaataata
     4081 taacaaaaag tccgcaatcc taattgaagc acgaccatcg ccgtaagggt ttacggcatt
     4141 tgccattcgg tcatactctt gtttgtcggt aagaagtctt gcagcttctt tatatatgac
     4201 ctccctgtcc gtgccaacta ttttaacggt gccggccttg accgcttccg gccgctcggt
     4261 ttcatgacgc aataccagca ccggctttcc tagagaggga gcctcttcct gaaggccgcc
     4321 ggagtcggtt agaataagaa agcttttggc catgagattg tgcatctcat cggtatctaa
     4381 ggggtcaagg agcagtatgc gctcatgctg gcctaggatc tgaaaaacgg tgtctcttac
     4441 ggcggggttt aaatgcacag ggtacactac tttaatatcc ctgaaactgt cggccaattt
     4501 tctaattgca tgacagatat tttccaaagg ctcacctaaa ttttcccttc tgtgtgctgt
     4561 cactaacacc actttttgtc tgtcgaaatc caagttgttc agtgcatcgg tggaaaaaac
     4621 aaagtcctct ttcaccgtag tcctcagggc atctatcacg gtattgccgg tgacaaagat
     4681 attttgagaa cttactccct cccgcaaaag gttttttcga gcagtccggg taggagcaaa
     4741 gtgcaaatct gccagggctc cggtgagctt tcgattcatc tcctcgggaa atggaagcca
     4801 tttgttgtga gtcctgagac ccgcttcaac atggcctacc ttaatcccat cgtaaaaagc
     4861 cgcaagggcc cctgcaaatg tggtagttgt gtcaccatgt actaaaacca tgtcgggttt
     4921 agagctatcc aaaacgtccc tcaagcccag gagggccttt gtggtgatgc tgaaaaggct
     4981 ttgcctatcg gtcatgatat ttaaatcgta atccacatta ataccaaaaa ggctcaacac
     5041 ctggtccagc atttgccggt gttgagctgt aacagctacc tcacactgaa aacaatccct
     5101 ttgctgaagc tccttaacca agggtgccat tttaatggct tccggccttg ttccaaagat
     5161 cgacaaaatc ttaattttat ccaagactta ttccccttta cattccatgg gttgattttt
     5221 tggtatctaa ggctatattt atatacctta cgctaacgag cagcataaag agtaccaagc
     5281 taagggcaat gacggcttgt tttacgccaa tccttgccag caccagcgaa catattccta
     5341 aggatatgct gattatatac atgaacaaaa ctgcctgctt ttgagatagg cctatggcta
     5401 tcaggcgatg atggacatgg cccttgtcag cctgcatcag gggcttgcca tttatgaatc
     5461 tacgcaatat ggcaaaggcc gtatcaaaaa tgggcagacc caaggccaag atggggacaa
     5521 tcagggctat ggccgtagcg ctttttacag ttccttccac agcctgtgcc gcaaggacga
     5581 aacctaaaaa cattgcaccg gtatccccca taaagatttt ggcaggattg aaattatagg
     5641 gcaaaaatcc gatagccgca ccggcaagta ttgccattac cagtgcgcca tttgcctgct
     5701 ccaggctaat gtttaccagc atcatggtaa atgcagcaat ggctgcgatc ccggctgcta
     5761 aaccgtcgag gccatctatg aaatttaacg tattggtaat gcccaccacc caaaacaagg
     5821 tagcaggaat ggccagtttc cccagaacaa acatgccgcc caagggattt gtaataatct
     5881 ccactttcac tccgaaagct accaaaattc cggccgccgc aatttgcccc agcagcttca
     5941 cccttgccgg caaattgtaa atatcatcta caatgcccac gataacaatc aggctggcgc
     6001 caaggaatat gccttgtata tatccttgct tcaaaggcag ggttaaaagg ccagttaata
     6061 caaaggcgat gtatatggcc agacctccga gcctgggcat gggttgttta tgaattcggc
     6121 gaggatcgcc gggtttgtcg atggcgccta ttttccaagc cagtttcatg acggaaggcg
     6181 taaccaggta tgccaaaatc atagaggcta aaaagccgta tatgtaccgt ggcatgaaat
     6241 cgctcccttt ataaggtctg tctctaacca ttttattgca tatacaaggg tttgtcaatt
     6301 gccgttagtt gccagctgct gagttcaggg cttttccctg ggcaaatgaa ctacttctat
     6361 ccagggtgcc tctttaaacc aatcgtaatc cacgtgatag tcctgggcat atactaccct
     6421 cgtaataccc gagttgatga tgagcttgga gcactccgca caggggcggg cggtaacgta
     6481 gatggtggcc cccttcctct cctcgggcga acattccagc agtgcgttca cctcggcatg
     6541 gatggtcctt acgcagtgat ttgccctcat catacagcca acatcgtcac aatgggccat
     6601 ctttgaagga ctcccgttgt aaccggtgcc ttttatcctg ttgtccttca ccacaacggc
     6661 tcccaccttg agcctggggc atgtagacct atcccttacc tgaaatgcta tgtccaaaaa
     6721 atactggtcc caatcttttc gcatgattgc ctccttacat tacctatctt attgtgacaa
     6781 gttttcgatc atccggtcta aaatttcaaa tacgtgtttt gagttttctt tggctatttg
     6841 gtactgattt ttggcctctt ccaggtctcc gtcccttatg gcaatcagtg cctgctgtcc
     6901 ggcattgtgc aggttctcgt gatactcgtc aatgcgttcc cacagctcat gtatggaacc
     6961 atcttttatt atcagggcgt tgtagaaatg gccgaaggcg catctgttgg aatcaacttg
     7021 caggggtatg agtttgccct catcaacagc ttctttcagt gtatccatcc agaggctgtg
     7081 ctgttttttg gcattttcca aaatgcctgc caattctttt ttcgtgacgg gattgttttt
     7141 atccatgaat ttttgatagt attccttgtt ttgggctata aagccatcat ccatataatt
     7201 aatctgatct ttaattgatt ccagacgctt tatatcactt tcaagggtat tgacgatgta
     7261 gactacctcc tcggtttccg tggaaagact gtgcatggcc gatgatacct cgctgacagc
     7321 ggaacttatc tgctggaatg aagcaacaaa gccttccata tcgcttttaa tcttgttgat
     7381 agcggtaaaa cttccttcaa ccgcttccct aattgcactt gtcacttccg gaatttgtgc
     7441 cattacttca ttggtccgct ccatactttc gaggctttgg gctgaatccc tatgtatttc
     7501 ctgggtaaag ctcttgaact tttccaagct ttccttggtg ctatccgcca atttccttat
     7561 ttcttctgaa accaccgcaa atcccctgcc cgcttctcct gccctggctg cttctatgct
     7621 ggcattgagt gccagcaggt ttgtctgatc cgcgatctcc tctatcacct caacgatgcc
     7681 gccgatttcc tttaaatgct ctaagagctt ggtcaaggtt tgattgaccg cactgttgct
     7741 ctcggttacc tttttgcaca cttcacccat tagttttaag ctctccatgt tcttgttgtt
     7801 gctctttaca atgttctcga tgttagcatg aatttcgtct atgtttttta cattgtcctc
     7861 aattgcagta tctatttcgc ccatggaggc agtggtttcc tgcacaaagg ccagggtgtt
     7921 ctcggactgc aagccgagct tgttcataat ggtctctatc tcatccatca aatgcttaat
     7981 atttacttct atgttcccta aggatgcgct caatgaaatg gtttttaata acatttccct
     8041 ggcatcatct tttaaatctt ctaactggcc tattttgcta aaaacctgtt tcaccttggg
     8101 attttgggat gctatgggcc tattgtttat tatggcatcc attaccttat cggtattaga
     8161 aaaaaacaat ttcatacccc tctcattatt catatcattt tctttcaaat gcggaaactt
     8221 ggaaaattta ctccgattaa tagtttagca cataatcgaa cataatttca atttaataaa
     8281 aattaactac tagtaaaaaa taaaggcctc ctaaaaagga cagcccttat tatatactta
     8341 ttttgttccg tacagcctat cgccggcatc gccgagaccg ggcacaatat aacccttgga
     8401 atttagcctt tcatcaatgg ccgcagtgta tatctcaaca tcgggatgtt ctttgtggac
     8461 agcctctatc ccttcgggtg ctgcaatcaa gcacatgagc tttatgctcg ttgcaccctt
     8521 gtcttttaaa acttctactg ccttagctgc agagccgccg gttgccagca tggggtcgag
     8581 aattatcagt tcccgctcgc ttacatcgct gggcaacttg cagtagtact ccacgggcat
     8641 caaggtttcg ggatcccg
//
LOCUS       DAONUO010000043         8472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6376_length_8472_cov_3.732209, whole genome shotgun sequence.
ACCESSION   DAONUO010000043 DAONUO010000000
VERSION     DAONUO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6376_length_8472_cov_3.732209"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PK768_02525"
     CDS             complement(<1..695)
                     /locus_tag="PK768_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQA59877.1"
                     /translation="MPAIYDTSIPIEEKLSILKKEVEHKGHIRMGIADASGTMMSTED
                     TSIDVSDRAYYIKSMNGENAVSDPIISKHDGSVILCFGVPIKENGQIVASMIAVREGT
                     TLSDVVDDINFGQSGKAFVINKDGTTIAHYDEEQVINMFNAIESSKEDLSYASLAQLK
                     KQMLEGHKGSSRYTYNGVTNIVGFAPIEGLDWYLAVSAPEDEVLASLGKIQAYMPLVF
                     MVFVLGGIALSYF"
     gene            complement(743..907)
                     /locus_tag="PK768_02530"
     CDS             complement(743..907)
                     /locus_tag="PK768_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59878.1"
                     /translation="MKSLKSKIVLLFVPLFIVAFLGLTVVLYNISATTIEAEAMNSMK
                     NVENTREQKS"
     gene            complement(1227..2003)
                     /locus_tag="PK768_02535"
     CDS             complement(1227..2003)
                     /locus_tag="PK768_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HQA59879.1"
                     /translation="MKNYEAYKMTANQFINGKYNKVILSIGSCEAHGYHLPLGTDTLV
                     AYKLACEVAEHYDDILALPPIPIGYSGHYDNFPFTLTFSYDTTIRVLYDVIASVLRNG
                     IKRIIIINGHDGNIAPIEIASRQIKEKYPDARIIALPQWWVTAGELLPKDTFEVWDGL
                     GHAGEGETSIMYYYYPQWCEPELAKSVVPDNLPPYVDVKWDFAEITDTGQTGDATKAS
                     AEKGKKMHDVLVKCLIDAIDYLNERDWNYNTTGHAKAKLD"
     gene            complement(2154..2561)
                     /locus_tag="PK768_02540"
     CDS             complement(2154..2561)
                     /locus_tag="PK768_02540"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HQA59880.1"
                     /translation="MSIEWTPDLSVGVKHIDDQHKIWFQKANELFEAGRQQRAKEYVN
                     TMIDFLDEYTKQHFRDEEAYMEKIGYPEIDAQKKAHASFIKDLAKLKEDFNKSGGNIL
                     VIINANKIVIDWLSNHIRKMDKKIGEYVRSLDQ"
     gene            2807..3907
                     /locus_tag="PK768_02545"
     CDS             2807..3907
                     /locus_tag="PK768_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQA59881.1"
                     /translation="MESIFSPQKVNLGRQLELDVARGLAIVFMVLIHSFELFMEHPFP
                     NTASTHIIRFLGSPPAAPVFMLLLGVGIVYSKRTSAAFLFNRGIMTLILAYALALSRD
                     LIPSYVAYLIKGDEEILIDGFLEFLGVDILQFAGLTFLFFALVIKLKFEIWQIVSCVF
                     VFSTFGAILGEKSTGNLGLDAFAGLFWGTWDRTWFPFFSWIFFPVAGYIFGHFLMHCK
                     NKTEMYKRIFIVSSVSLVPLVILSYDYRIEFGAFGDLFLQQYYHQDLFGNIILTIFSL
                     FWISLLYFIVTSLKNCSFHTLQRWSKNVNQIYVTHWIIYGILCQIIENTLSVLEIVVL
                     AAVVLIVSDLIATQYLKFKSKGYSFRFVIGKS"
     gene            3940..4074
                     /locus_tag="PK768_02550"
     CDS             3940..4074
                     /locus_tag="PK768_02550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQA59882.1"
                     /translation="MGDECLRQIASVLTKCVNRSVDLAARYGGEEFACILPDTAFTVQ
                     "
     gene            4071..4292
                     /locus_tag="PK768_02555"
     CDS             4071..4292
                     /locus_tag="PK768_02555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQA59883.1"
                     /translation="MKVAERIRQRIHDLKIEHTESPVSKYVTASFGVTTVRYSLKSSP
                     MEIIAFADKLLYKAKVSGRDMIEYTKFRS"
     gene            complement(4303..5361)
                     /locus_tag="PK768_02560"
     CDS             complement(4303..5361)
                     /locus_tag="PK768_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HQA59884.1"
                     /translation="MLFLIIGVLLFIASSHFPYITERIYSSFIYKKIIRSLSLITSPL
                     PFSLAEMLIVLSVIIAIAYIIRFGTCLAKTKETKNLKVLFQNIVTLVGIIYLTFQLLW
                     GLNYNRLMISEILDLNTRTPTQQELIGLCEDLIAKANRLRRDLNEDESNIMKLPYGKD
                     GALRIAYMGFERVSSFYPDFEGRYGRPKGIVLSEYMSYTGITGFFFPFTGEANVNMHI
                     PDSSLPFVIAHEMAHQLGFAREDEANFIAYIACINHPDIYFQYSGTLSALGYSMSALR
                     KVDHVKHNDLLAAYSEAVRRDLQYNNKFWARYSGPIERASNKINDTYLKSQNQKTGVK
                     SYGAMVDLLIAERRKNTK"
     gene            5589..6344
                     /locus_tag="PK768_02565"
     CDS             5589..6344
                     /locus_tag="PK768_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQA59885.1"
                     /translation="MWKSVRINNKSVSALTLFSVVLVVMALVTSCASNSEVSKSNESD
                     SEQGNLPDGFVYVTDVIPTAQLEIRYFSEDNFVGAVIDGYEAPKAILTVEAAEALKKA
                     ADILEEQGYYIKIFDAYRPQRAVDHFVRWSKDLDDTKMKEKYYPDLDKSVLFELGYIA
                     EKSGHSRGSTVDLTLVEISSGKELDMGSGFDFFGDISNHGTHLITPEQEKNRNILRDA
                     MVETGFKVYPEEWWHYTLENEPYPDTYFDFPVK"
     gene            6508..6816
                     /locus_tag="PK768_02570"
     CDS             6508..6816
                     /locus_tag="PK768_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA59886.1"
                     /translation="MSNKSANIQKIEYLAKLKLQSTVVGRHEEIEAMKMQFDKGLEGH
                     SAVTVIAGDVGIGKTALIKTGLSDLSKLNCVSAYGKFEQYEDKGPYIAITQIIEQMHN
                     "
     gene            6839..8068
                     /locus_tag="PK768_02575"
     CDS             6839..8068
                     /locus_tag="PK768_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HQA59887.1"
                     /translation="MSGDLNVGGLVGCVNSEDVVRDPSFSDIIKSYASGTVSGSKHVG
                     GLVGNNYGRVEYSHSIGNVKGETYVGGLVGFNSYYVEDCYAAGLTEGKVNIGGLVGFN
                     DAYVMSSNASGTTEGETYVGGLVGFNYGLVEYCYADTNVFAKTYVGGLVGYNDGTTEG
                     SYASGNVMGEDFVGGLVGYNIYEVTDSYATGTVFGSIAVGGLVGINKDQVTNCYAIGL
                     VYGGTVQGGLIGINDGGVTSSYYDKETSGQSDTGKGEPKTTAEMKLRSTYQDWNFEYV
                     WSIRDGETYPMFYWQEEAPQSSFVVEIENIGALSGMAFNLYINYARGEDGELLNGFYR
                     VKVDSDLDGENVVNEEFIFIDGETTVPVTLDAVGIHKLRIYVTRISFSNVVEINKKVI
                     SVTTESDSSLLPKVLEM"
     gene            8065..>8472
                     /locus_tag="PK768_02580"
     CDS             8065..>8472
                     /locus_tag="PK768_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59888.1"
                     /translation="MTFKAKDSEGMEHSFVNEGYWGPEGGFDIPAEYEATTEWKLVFS
                     KAGNYTITFSLIDADTEQVIADITDTVEINVKHAESGGSGRKDESPKPETKPSDESRI
                     TITTELDRNKGVASGQIDEKTLKEAIDEIIVAIP"
BASE COUNT         2487 a         1535 c         1786 g         2664 t
ORIGIN      
        1 atgaaatagg aaagagcaat accgccaagg acaaaaacca taaaaacaag aggcatataa
       61 gcctgtattt tgcctaggct tgccaacact tcatcttctg gcgcacttac ggctaaatac
      121 caatccaatc cctcaatggg tgcgaaacca acgatgttag taactccatt atatgtataa
      181 cggctgcttc ccttatggcc ttccagcatc tgctttttca actgggcaag agacgcatag
      241 gacagatctt ctttggagct ctctatggca ttaaacatgt ttataacttg ctcctcgtca
      301 taatgagcta ttgtggtacc gtctttattg atgacaaatg ctttgccgga ctgcccaaag
      361 ttgatatcgt caacaacatc acttaacgtg gtaccttctc tgacagctat catggatgcc
      421 acgatttgac cgttttcttt tattggaact ccaaagcata gtataaccga gccatcgtgt
      481 ttgcttatga ttggatctga tactgcattt tcaccgttca ttgacttaat ataataggct
      541 ctatcactga catctattga tgtatcttcg gtagacatca tggttccgct ggcatctgct
      601 atgcccatcc tgatatgtcc tttgtgttca acttcttttt taagtatcga caatttttct
      661 tctattggta tgcttgtgtc atatattgct ggcattgcag ctaaactctc tagggaagcc
      721 aattcggcat ttaagcggct ttttacgatt tttgctccct ggtattttca acatttttca
      781 ttgaattcat cgcttctgct tcgatagttg tagccgaaat gttatacagt acaacagtca
      841 ggcctaaaaa tgcaactatg aacagtggta caaaaagaag cacgattttt gatttaagac
      901 ttttcataag ttactcctcg cttccgatat cttaatttgg catttgcttt tgtttgtctc
      961 acaatttcca ttaattagga cgaattatac tatattagta tagtcgaatc aataattttt
     1021 ttatcgaagc atgtaaaaaa tttttcacag caggtcgcta tttgtcttaa ctttgatgca
     1081 aagtgtgtaa cattggtacc gatggttaaa aagtgaatgt gggaactgcc cggtaaataa
     1141 tgatttttat cacgccttta gaagatgagg taagaaaaca ataaaaatcc tctgatgatg
     1201 agttttcatc agaggatttt tattactcaa tctaatttag cttttgcatg gcctgttgta
     1261 ttgtaattcc agtctctttc atttagataa tcaatggcat ctatcaagca cttaaccaat
     1321 acatcgtgca tctttttgcc tttttcagca gaggctttgg tagcatctcc cgtttggccg
     1381 gtgtcggtaa tctctgcaaa atcccacttt acatctacat atggaggcag attatcggga
     1441 actactgatt tggcaagctc gggttcgcac cactggggat aataatagta catgattgag
     1501 gtctcgcctt cgcccgcatg gcccaagcca tcccaaacct caaaggtgtc ttttggcagt
     1561 agttctcctg cagttaccca ccactgcggt agagcaatta ttctggcatc cggatatttt
     1621 tcttttatct ggcggctagc tatttcaata ggagctatat ttccgtcgtg gccatttatg
     1681 attatgattc tttttatgcc atttcttaga acggatgcta tcacgtcata taacactcta
     1741 attgtcgtat cataactaaa tgtcaatgta aagggaaagt tatcgtaatg gccactatac
     1801 ccaatgggaa ttggagggag tgccagtatg tcatcataat gttcggccac ttcgcaagcc
     1861 agcttgtagg ccaccagtgt atcagttcct agcggcaagt ggtacccgtg ggcctcgcag
     1921 gaaccaatgg ataaaattac cttgttatat ttaccattga taaattgatt cgccgtcatt
     1981 ttatatgctt catagttttt caccttctca tcccccttta attaaacttt gaatattaca
     2041 caaattacca atgttatagt atcaacttcc taaaattctg tcaatacatt ggtggagcca
     2101 tgccgatcaa cattaaaaaa tggccaccct gtaggacagc catttttaac gaattattga
     2161 tcgagacttc taacatactc tcctattttc ttgtccattt ttctgatgtg attagaaagc
     2221 caatctatga ctattttatt tgcattgata atgaccagta tattgccgcc agacttatta
     2281 aagtcttcct ttaatttggc aagatccttg ataaaactcg catgtgcttt cttttgagca
     2341 tcaatttcag gatagccaat tttctccatg taggcttctt catccctaaa gtgttgtttt
     2401 gtgtattcat ctaagaaatc gatcattgta ttgacgtatt cttttgctct ctgttgtctc
     2461 ccggcttcaa acagctcatt agccttttga aaccatattt tgtgttgatc atcgatatgt
     2521 ttgacaccta ctgacaaatc tggagtccat tctattgaca taaagaccct cctcgcagat
     2581 tattcttatt acctagattt tataggtttt tttagataat ttcaatagat ttccaagaga
     2641 aaggggtatg tatgtctaga tttgtaaggg aaagtatgcc taatagctag aatttgacat
     2701 ctatttataa tatttgacat ttttttcgct tacttattga aaaaatagag acgagatgaa
     2761 ataataagaa taataaaatt aaattaaata tatggggggc ggatttatgg agagtatttt
     2821 ttctcctcaa aaggttaatc tcggcaggca gttggagctg gatgtagcaa gaggtcttgc
     2881 tattgttttt atggtgctga tacattcatt tgagcttttt atggagcatc cttttccaaa
     2941 tactgcctct actcacataa ttagatttct cggttctcca cctgcagcac ctgtatttat
     3001 gttactattg ggtgtaggta ttgtctattc aaagagaact tctgctgcct ttttatttaa
     3061 caggggtata atgacattaa ttctcgctta tgcactagct ttaagccgcg atttaattcc
     3121 ctcgtatgtt gcttacctta taaaaggtga tgaagaaata ctaatagacg gttttcttga
     3181 gtttctgggt gttgatattt tacagtttgc cggtcttact tttttgtttt tcgctttagt
     3241 tataaaactt aaatttgaaa tatggcagat cgtttcctgc gtgtttgttt ttagcacatt
     3301 tggtgcaatt ttaggtgaaa aatctacagg aaatctcgga cttgacgcgt ttgctggtct
     3361 attttgggga acttgggata gaacatggtt tccctttttc tcatggatat ttttccccgt
     3421 agccggatac attttcggtc attttcttat gcattgcaaa aataagacgg aaatgtataa
     3481 gaggatattt atagtaagct ctgtttcact agttcccctt gtaatactca gctatgatta
     3541 cagaattgaa tttggagctt ttggagactt atttctgcag caatactatc accaggacct
     3601 cttcgggaat ataattctta caatcttttc actattttgg atcagtctat tatattttat
     3661 cgtcacgtca ttaaagaatt gtagttttca tactcttcag cgctggagca aaaatgtgaa
     3721 tcaaatatat gttactcatt ggattattta tggaattctg tgtcagataa tagaaaatac
     3781 cctaagtgta ttagaaattg ttgttttagc agcagtcgta ttgatagtgt cggatttgat
     3841 tgcaactcaa tatttaaagt ttaaatcaaa agggtattcc tttagatttg ttatcggcaa
     3901 atcctagttc tatagcaaaa ctattttcac ggccatgtca tgggagatga atgtttgcgt
     3961 caaatagcca gtgtattgac gaagtgtgta aaccggtctg ttgatttggc ggcccgttac
     4021 ggaggagaag agtttgcctg tatactgcct gatacagcat ttacggtgca gtgaaagtag
     4081 ccgaaaggat tcggcaaaga attcacgatc taaaaataga acatactgaa tctcctgttt
     4141 caaagtatgt tactgcaagt tttggagtaa ctacagtgcg atattcatta aagagctcgc
     4201 caatggaaat cattgctttt gccgataaat tgctgtataa agctaaagta tccggccgcg
     4261 acatgataga atacaccaaa ttcaggagtt gattaagtag gcctatttcg tgttttttcg
     4321 tcgctctgcg atcaataaat ctaccatcgc accataagat ttaactccag ttttttggtt
     4381 ttggcttttt aggtaagtat cattgatttt gttcgaagcc ctttcaatag gaccggaata
     4441 tctagcccaa aatttattat tatattgcag atcgcgtctt actgcttcac tatatgcggc
     4501 aagcaggtca ttgtgtttta catgatctac ttttcttaag gcgctcatag agtagcctag
     4561 agctgaaagt gttcctgaat attgaaagta aatgtcggga tggttaatgc atgcgatata
     4621 tgcaataaaa ttggcttcat cttcgcgagc aaaacccaac tgatgtgcca tttcgtgggc
     4681 tatgacaaag gggagggaag agtcgggtat gtgcatattg acgtttgctt cgccggtaaa
     4741 agggaagaag aatccggtga ttccggtgta tgacatatat tctgaaagca ctataccttt
     4801 tggcctaccg tacctcccct caaaatcggg ataaaacgat gaaacccttt caaatcccat
     4861 ataggcaatt cttaaggcgc cgtctttgcc gtaaggaagc ttcattatat tactctcatc
     4921 ctcgtttaaa tctcttctca aacggtttgc ctttgcaatc aaatcttcgc agaggcctat
     4981 cagttcttgc tgtgtaggcg ttcttgtgtt caaatccagt atttcactta tcattaatct
     5041 attgtaattc agcccccaga gcaattgaaa agtgagataa attattccta caagggtcac
     5101 tatattttga aataatacct ttaaattttt tgtctctttc gtttttgcaa ggcaggtgcc
     5161 aaatcggata atgtaggcta tcgcaatgat tactgacaac acgataagca tttctgccaa
     5221 tgaaaaagga agcggacttg taatcaggct taagcttcga atgatttttt tgtaaataaa
     5281 tgaagaatat atcctctcag taatataggg aaaatgtgat gaagctataa atagtaatac
     5341 gccgatgatt aagaaaagca aaacacatca cagccttttt aaaatcacaa aaatgttttg
     5401 gtgtaaggaa acacccaaaa aactactgta gtagcacttt ttaatataat agccccaacg
     5461 gatttaaaaa tcaataaatg aaaaaagtcg aaagatgtat gtaaagagtc ttgcttttgt
     5521 atttttttag aatataatag aaattgattt gtagggcttt tttattttat attgaggagt
     5581 gatgttcgat gtggaaatct gtaagaatca acaataaaag cgtatcggcc ctgacgctat
     5641 tttcagttgt tttggtcgta atggccttgg ttacctcatg tgcctccaat tcggaggtct
     5701 ctaaatcaaa tgaatctgat tcggaacaag gcaatcttcc ggatgggttt gtttacgtaa
     5761 ccgatgtaat tcctactgca cagcttgaga tacgctattt tagcgaagat aattttgtgg
     5821 gtgcggttat agatggatat gaagccccga aagctattct taccgtagag gcagcagagg
     5881 cccttaagaa agcggcggat atacttgaag aacaggggta ctacataaaa atatttgatg
     5941 cctaccgccc acaaagggca gttgaccatt ttgtgcgctg gtcgaaagat ttagatgata
     6001 ctaaaatgaa ggaaaaatac tatcccgatt tggacaagtc ggttctgttc gaacttggct
     6061 atatagctga aaaatccggc cattcgagag ggagcacggt agatttgact ttggttgaaa
     6121 tatcttcagg caaagaattg gacatgggca gtggctttga cttctttggt gatatttcaa
     6181 accatggcac ccatttaatt acccccgagc aggagaaaaa ccgaaatatc ctacgggatg
     6241 ctatggtaga aactggattt aaagtatatc ctgaagaatg gtggcactat acacttgaga
     6301 atgaacctta tccagataca tattttgact tcccggtcaa ataaatcaat gatagttaaa
     6361 accgcagttt gcagttaatc cgaggattgc attctgcggt ttttgtatat atgtcacttt
     6421 ttgtagtgag caatgtggaa acatgtggta taatatttaa tgactattag aaaataaaca
     6481 atattaccat ttaaaggata agggcatatg agcaacaaga gtgcgaatat acagaaaata
     6541 gaatatctag ccaaattgaa actgcaaagt accgtagtgg gaaggcatga agaaatagaa
     6601 gcaatgaaaa tgcagtttga caagggactg gaaggccatt ctgcggtgac agttattgcc
     6661 ggtgatgttg ggattggaaa aacggcactg ataaagactg ggctgtcaga tctatccaag
     6721 cttaactgtg tgagtgccta cggcaaattt gaacagtacg aagacaaagg gccctatatt
     6781 gcaataactc aaataataga acaaatgcac aattagttat tctaatgcca gcggaaaagt
     6841 gagtggcgac ctaaatgttg gagggcttgt aggatgtgta aattctgaag atgtagtaag
     6901 agacccaagt ttttccgata ttataaaatc ttacgccagt ggaactgttt ccggtagcaa
     6961 gcacgttgga ggcctagtgg gtaataatta cggcagggtc gaatactctc attctatagg
     7021 gaacgtgaaa ggagagactt atgtcggtgg tttggtagga tttaacagtt attatgttga
     7081 agattgttat gctgcagggc tcacggaagg aaaggttaat atcggcggtt tagtaggctt
     7141 caacgatgcc tatgttatga gcagcaatgc ttctgggacc acagaaggtg agacatatgt
     7201 cggtggtttg gtgggcttta attacggctt ggttgagtac tgttacgctg acacaaatgt
     7261 ctttgccaaa acttatgttg gcggtttggt aggctataat gacggtacta cagaagggtc
     7321 atacgctagc ggtaatgtta tgggagaaga ttttgtcgga ggtctggtcg gttataacat
     7381 atatgaagtt acagattctt atgcaacagg taccgtattt ggaagtatcg ccgtgggagg
     7441 tttagtagga atcaataaag accaagtaac taactgttac gccatcggac tagtatacgg
     7501 tggaaccgtt cagggtggct tgataggtat aaacgacgga ggggttacta gttcttacta
     7561 tgacaaagag accagcggtc aatcggatac cggcaaaggg gagccgaaga ctaccgcaga
     7621 aatgaagctt cgctccacct atcaggactg gaacttcgaa tatgtatgga gtataaggga
     7681 cggggaaact tatcccatgt tttactggca ggaagaagcg ccacagtcga gctttgtggt
     7741 ggagattgag aatatcggag ctctttcggg catggcattt aatctatata taaattatgc
     7801 caggggtgag gatggcgagc tgctaaatgg cttttatcgg gtgaaggtcg atagcgacct
     7861 ggacggtgaa aatgtagtaa atgaagaatt tatattcatc gatggtgaaa caacagtacc
     7921 cgtaacatta gatgcagtag gaatccacaa gctccggata tacgttacac ggatatcatt
     7981 ctccaacgtt gtagagatta acaaaaaggt tatttcggtt acgacggagt cagattcaag
     8041 tttgctgccg aaggtcctgg agatgtgacc ttcaaggcga aagacagcga aggtatggag
     8101 cacagctttg tcaacgaagg ctactggggt ccggaaggtg gatttgacat acccgcagaa
     8161 tatgaagcca cgaccgagtg gaaactggtc tttagcaagg caggaaacta taccatcacc
     8221 ttcagcctta tagatgccga tacagaacaa gtgatagcgg acattaccga tactgttgag
     8281 attaatgtaa aacatgccga aagtggtggc tctggtagga aagatgaaag tcctaaacca
     8341 gagacgaaac catctgatga aagcagaatt acaataacta ccgaattaga cagaaacaaa
     8401 ggtgtggcat cgggccaaat cgatgagaaa acattaaaag aagccataga tgaaattatt
     8461 gttgccattc ct
//
LOCUS       DAONUO010000044         8336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6506_length_8336_cov_3.451999, whole genome shotgun sequence.
ACCESSION   DAONUO010000044 DAONUO010000000
VERSION     DAONUO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6506_length_8336_cov_3.451999"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PK768_02585"
     CDS             <1..965
                     /locus_tag="PK768_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQA59889.1"
                     /translation="LVVAKPSTSGATTSPELVEGTVQYLKSKGFDNIIIMEGSWVGDR
                     TSRAFEVCGYTQIARKYNVPLIDLQKDSYTTCYVDGMEINVCEQVLKVDYLINMPVLK
                     GHCQTKITCALKNLKGCIPNSEKRRFHSMGLHKPIAYLNKAVKSHLIIVDGLMGDLDF
                     EEGGNPVQMDRIIVGFDPVLIDSYVADLMGYDAEEITHIKIAKEIGVGNCLASSNQIY
                     ELNEDFNTRKIPRSRAVERLSKYVEERDACSACYGSLIHALARLDEQGLLRKLKGKIC
                     IGQGFKRVEGKGIGVGACTRYFEQNVPGCPPTAKDIIVLLKKDI"
     gene            1047..2483
                     /locus_tag="PK768_02590"
     CDS             1047..2483
                     /locus_tag="PK768_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQA59890.1"
                     /translation="MKNNMTPDEKFQMMTQTPVEKLIVTLAIPTITSMLITSIYNMAD
                     TFFVSKISTSASGAVGVAFSLMSIIQAVGFTIGMGSGNYISRLLGQKKREHAAQVAAT
                     GFFTALILGVVITVIGLIFLDTLVYALGATPTIAPYAKAYIKFILLGAPFMTASFVLN
                     NILRFQGSAYFAMKGITTGGIINIILDPIFIFGLNMGTAGAAIATIISQFISFSILYY
                     NSSRGGNIKISSRNFTPKWEVYKQILKGGMPSFYRQTLGSIAMIFMNRSAGVYGDAAV
                     AAMAIVTRVFQFVILVMIGFGQGFQPVCGFNYGAKRYDRVLRAFWFSVKVSVTFLVFM
                     AAIGFVFSSNIVAAFRKEDLEVIAIGTRAFRYYCIAFPLSSWVVMTNMLLQTIGRSFE
                     ASIVAMSRQGLFFLPAILILPRKLGLLGVQISQPISDLCSFLIALLLGLRVLNELKSL
                     HEEVEIAKGDKCGEVYISCLKESRAHQN"
     gene            complement(2578..2991)
                     /locus_tag="PK768_02595"
     CDS             complement(2578..2991)
                     /locus_tag="PK768_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HQA59891.1"
                     /translation="MIEMKVKTVTVDIGGNFTVLLVDKEKEKMVLPIVIGGLEAHNIV
                     MPIQGVEPPRPMTPDLLKTAIEKLGGVVEKVVITSIKGNTYYAEIHINQNGQSIVLDS
                     RPSDAIALAVRCDIPIFMNRWLVEFTYDTSDIQLS"
     gene            3613..4257
                     /locus_tag="PK768_02600"
     CDS             3613..4257
                     /locus_tag="PK768_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQA59892.1"
                     /translation="MFQDRQDAAMQLAEKLKTYKNDPASIVLAIPRGGVIIADVVCEQ
                     LNLPMDIVVTRKLGAPYNEELAIGAVDSRGGTILNHNIIFRLRVPEAYIEIEAKRKAE
                     EARTRLKQYRGTDEYESLSDKNAIVVDDGIATGYTVMSAINFLKGLKPKKLILATPVI
                     APDTRVKMEKIVDELICVKSEDPFYSVGQFYYKFFQVTDSEIMDVLAKRRFPRQ"
     gene            complement(4341..5003)
                     /locus_tag="PK768_02605"
     CDS             complement(4341..5003)
                     /locus_tag="PK768_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQA59893.1"
                     /translation="MKVEFLGHAAFLITEESYTLLFDPFLTGNPLAAKKADEVKVTHI
                     FVSHAHSDHLGDAIQIAKNSDATVYAVFEVAQIFEKEGIKVQAGNLGGRLPADFGSVK
                     IFQAFHSSGVAGGSASGFVVELIGKKIYFAGDTALFGDMSLLESEEIDLALLPIGDIY
                     TMGIEDAAKAVEMIKPKKVIPMHYNTFPAIQRDPMEFKKLVEARTQTEVIILKPGESV
                     EI"
     gene            5133..6191
                     /locus_tag="PK768_02610"
     CDS             5133..6191
                     /locus_tag="PK768_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HQA59894.1"
                     /translation="MGFRFRKSIKLAPGVRLNLGKKSASISFGTRGLRHTISTSGRRT
                     VSIGVPGTGIYYTKSSKVGSKLGSKSAARDDSADPYQEVAEFEEALEYITSLHEKCDY
                     EYDWEAIAKEPPPFKAGDKGPNELSALEKLESFKPSILGRVFKSLEAKRRAELEEEVI
                     KAVEEDKKLYEAWEKQRRLAESVLNRDTRAYGTLLKDIKFCSELAGLVNSFSFQIPDA
                     DSMIIDCHINLEDVIPTHYKTLTKTGRLSIRKYSKTDYHAISKLYLTSLMWRIARNIL
                     GLLPLQSVTINILTCVVDTSLGQVMNITTLSVKIDKSIFDKLNFDLIDPFDALVNFEH
                     NVRFLKTKGFQAVKKLND"
     gene            6215..7045
                     /locus_tag="PK768_02615"
     CDS             6215..7045
                     /locus_tag="PK768_02615"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HQA59895.1"
                     /translation="MLLGAFISISKGYEHALKEALSIGANTMQFFTRNPRGSAAKALD
                     AVDIMKMKALIKETSFGPIVAHAPYTLNLASGKKEAREFSMRVLREDLERMEVVGALY
                     IVLHPGSHVGAGVDRGIRKIASALKEFACGREKVMVLLETMSGSGSEVGYTFEQLYEI
                     MDLTGNPDAFGVCFDTCHVLGAGYDIVESLDEVLFDFDKILGLSKLKVVHLNDSKFPL
                     GSRKDRHASLGEGELGLDAIKRIITHPKLQDKPFLLETPGGIENYKKEIKMLRAVAEN
                     "
     gene            7250..7669
                     /locus_tag="PK768_02620"
     CDS             7250..7669
                     /locus_tag="PK768_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA59896.1"
                     /translation="MVKILFERDLCLGCKTCELACAAAHSKTKSIAGAIRENKISRIR
                     IKTKGGKLKLEQCMLCKNPRCISVCPTRALSKDEESGIILVDEMSCTGCGLCEDACPF
                     GAIELHTFPTICDRCAHSEEPLCALSCPTRALKVKIV"
     gene            7688..>8336
                     /locus_tag="PK768_02625"
     CDS             7688..>8336
                     /locus_tag="PK768_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HQA59897.1"
                     /translation="MSGDFKCLSCDAANYKLMEKAKNENIETVWDRAEAMQPQCGFGE
                     MGLCCTNCSMGPCRIVKVSEDGPKKGICGADEDTIAARNLARAIAAGSAAHSDHGRDI
                     AHALHYAAKNPESGYEVKNSEKLISLAKEWGIETENLDIEDIAAKVSEVALQEFGKPF
                     GHLRFTERATKGRLDIWNKMNVLPRAIDRDIVEVLHSTHIGCDADYRNIMTAATRA"
BASE COUNT         2506 a         1609 c         1882 g         2339 t
ORIGIN      
        1 acctggtagt tgccaaaccc tccacatccg gagccacaac atcgccggaa cttgtagagg
       61 gaaccgttca atacttaaaa tcaaagggct ttgacaatat aataatcatg gagggttcct
      121 gggtgggcga tagaacctca agggcctttg aagtgtgtgg atacactcaa attgcaagaa
      181 aatataatgt tcccctcatt gatttgcaaa aggactctta tacgacgtgc tatgttgatg
      241 gaatggagat aaatgtatgt gagcaggtgc ttaaagtaga ttatttaata aacatgccgg
      301 ttttaaaagg ccattgtcag accaagataa catgtgcatt aaaaaattta aaaggctgca
      361 ttcccaatag tgaaaagaga agatttcaca gcatgggatt acataaaccc attgcatatc
      421 tcaataaggc ggtcaaaagc cacttaataa tcgttgacgg cctaatgggc gatttagact
      481 ttgaagaagg gggaaacccc gtccagatgg acaggatcat agttggtttt gaccctgtgt
      541 tgattgattc atatgtagca gatttgatgg gctatgatgc agaggagata acgcatataa
      601 aaatagctaa agaaataggt gtgggcaatt gccttgcttc aagcaaccaa atatacgagc
      661 taaatgagga ttttaatacc aggaaaatac cacgctcccg tgcagtcgaa agattgtcaa
      721 agtatgtgga ggaaagggat gcgtgctctg cctgctatgg cagcttaatt catgccttgg
      781 caagactcga tgaacagggt cttttaagga aacttaaagg aaagatttgt attggccagg
      841 gatttaagcg agtagagggc aaaggtatcg gggtaggagc ctgcacaaga tattttgaac
      901 aaaatgtacc gggttgtcct ccaacggcca aggatattat cgtgctgctt aaaaaagata
      961 tatgagaatc attcttaatt catgttataa tgttagtatc tgcaaatcta gcaagtataa
     1021 cttgatttat agagagagtg ttatccatga aaaacaacat gactcccgac gaaaaatttc
     1081 aaatgatgac acagactcca gttgagaaac tgattgttac actagccatt cctactatta
     1141 caagcatgct gattacttca atatacaata tggccgacac cttttttgtg agtaaaataa
     1201 gcaccagtgc ctcaggtgct gtaggtgttg ctttttccct aatgtcgatt attcaggcag
     1261 tcggctttac catcggcatg ggttcgggaa attatatttc aagactcctt ggtcaaaaga
     1321 agcgagagca cgctgcacaa gttgcagcta ccggtttttt tactgctctt attttgggcg
     1381 tagtaataac ggtaattggc cttattttct tggacacact tgtgtacgct ctcggagcta
     1441 ctccaaccat cgctccttat gcaaaagctt atataaagtt catcctgctt ggtgcacctt
     1501 tcatgaccgc atcttttgta ttaaacaata tcttacgctt ccaaggcagt gcatactttg
     1561 cgatgaaggg aataacaacg ggaggtatta ttaacattat attagaccct atattcatct
     1621 ttggtctcaa tatgggtacc gccggtgcag ctatagccac aattattagc caatttatca
     1681 gttttagcat tctatattac aattcgtcaa gaggtggaaa tataaagatc agttctagaa
     1741 actttacccc gaaatgggaa gtttataaac aaatcctgaa aggtggtatg ccgtcttttt
     1801 atcgccagac actggggagt attgccatga ttttcatgaa ccgcagtgcc ggtgtctacg
     1861 gtgatgctgc tgttgccgcg atggcaatag taacacgagt atttcaattt gtgatattgg
     1921 taatgattgg gtttggccaa ggctttcagc cggtgtgcgg gtttaactat ggggcaaaac
     1981 gatatgatag ggtgctgcgt gccttctggt tcagcgttaa agtttcggtg acattcttgg
     2041 tgttcatggc tgcaatcggt tttgtgttct catctaatat agtggcagcc tttcgaaaag
     2101 aagatttgga ggttatagca atcggaactc gcgcttttag gtattattgc attgcgttcc
     2161 cgctttcttc atgggttgta atgactaata tgctgcttca aacaataggc agatcatttg
     2221 aggcttcaat tgttgcaatg tcacggcagg gcctgttttt cttaccggca atcttgattt
     2281 tgcctcgcaa gctgggcctc cttggtgttc aaatcagcca gcccatatcg gatctttgct
     2341 catttttaat tgcattgctg ctagggcttc gcgtattaaa tgagctaaaa tcgctgcatg
     2401 aagaagtcga aattgccaaa ggagataaat gcggtgaagt atatatatcc tgccttaaag
     2461 aaagcagagc acaccaaaac taatacattt tgccaattga aatagagacc tcgcctttta
     2521 agggaggtct ctattttaca tataaagtgt ttgaattttt gctataatat taacatacta
     2581 tgataactgt atatccgacg tgtcataggt gaactctaca agccatctat tcatgaatat
     2641 aggaatatca catcttactg ctaaggctat ggcatcgctt ggtctggaat ctagcacaat
     2701 ggattggcca ttttggttta tgtgaatttc agcataataa gtattacctt ttatactagt
     2761 aatgacaact ttttctacaa ctccgccgag tttttcgata gctgttttta aaagatcagg
     2821 agtcattggc ctgggtggtt ctactccctg aatgggcata actatgttgt gagcttccag
     2881 cccacctata actataggga gcaccatttt ttctttctcc ttgtcaacca acaggactgt
     2941 aaaattcccg ccaatgtcga cagttacagt tttgaccttc atttcaatca tatgattcac
     3001 ctcagtaaaa tgatgaggta aaaattttcc tctaaatcta ttataacaat aacacatatt
     3061 ggtagcaaat aaaaaggcaa ccttgcggtt tgcctggtgt ctcactgaca acctttctat
     3121 atagtgtaat aacccgtatg ggacaatcat catctgctcc gctgtacagc ggattcctac
     3181 tttttgacac acagtcaaac attatgttgc tgatagtatt tttgctgaat caatccaacc
     3241 ctttacacat tctttttata tatctgtctc atatcttatt tatgtgctca ccttttaata
     3301 ttttagttgt tccaaatctc caccaggcct tcttttaggt caacatcgtt gatgatcttg
     3361 tcttcaattt cccaacagtc aaaaaactat aaaaatcttt taaatctagc attttttatt
     3421 aacgattaat tattacaggt atggggataa aaccaggcgt aatatgccga cactggtata
     3481 cagaaggtca aatgatttca ttagtcgaga ttgtgtgtta aaataaagtt aaaacaatga
     3541 attaattgtc gattcagaat ctaatatcgt gatttccaag caaactaata ttttgttgaa
     3601 aggaggcttt taatgtttca ggatcggcag gatgcggcaa tgcaattggc tgaaaagtta
     3661 aagacatata aaaatgatcc ggctagcata gtattagcca tccctagagg tggtgtcatc
     3721 attgccgatg tggtgtgtga acagctgaat cttcccatgg atattgtggt tacaagaaag
     3781 ctgggagccc cctacaatga agagctggcg ataggtgcag tagattcaag gggcggtacg
     3841 attttaaatc acaatattat cttcaggctc cgcgtacccg aggcatatat tgaaatagag
     3901 gctaagcgta aggctgaaga ggctagaact aggctgaaac agtacagggg aacggacgag
     3961 tatgaaagtt tgtcggacaa gaatgctatt gttgttgatg atggtatagc caccggatat
     4021 accgtaatgt cggcaataaa ttttttgaaa ggcctaaagc caaaaaagtt gattttagcc
     4081 acgcccgtta tagcgcctga taccagggta aagatggaaa aaatagtgga tgagttgata
     4141 tgcgtaaaat cggaagatcc cttttattct gttggccagt tttactacaa gttctttcag
     4201 gtaactgatt ctgaaatcat ggatgtcttg gcaaaaagaa gattcccaag gcagtgacca
     4261 tgttctgtcg tttttaaaat taaaattcga agattaataa aaaaggggct cttgcattct
     4321 tttgcaaaag ccccttctcc ttatatttct acagattcgc cgggttttag gatgattact
     4381 tccgtttggg ttcttgcctc caccagcttt ttaaattcca tggggtccct ttgtatggca
     4441 gggaaggtgt tgtagtgcat aggtatcacc tttttgggct tgatcatttc cactgccttg
     4501 gctgcatctt caattcccat ggtgtagata tcgcctatgg gcaagagtgc taaatctatt
     4561 tcttcacttt caagtaaact catgtcacca aaaagggccg tatctcctgc aaagtatatt
     4621 tttttgccta ttaactctac tacaaaacca gaggcactgc cccctgccac tccgctgctg
     4681 tggaaggcct ggaaaatctt cacgctgcca aaatccgccg gaagccttcc gcccaagttg
     4741 cctgcctgga cctttattcc ttccttttca aagatttgcg ccacttcaaa aacggcatat
     4801 actgtagcat cactattttt ggcaatttgt attgcatcgc ctagatggtc agaatgggcg
     4861 tgggagacaa aaatgtgcgt aacttttact tcatcggcct tttttgctgc caggggattt
     4921 cccgttaaaa aggggtcaaa gaggagggtg tagctctctt ccgttattaa aaaagctgcg
     4981 tgacccaaga attcaacctt catcaaaatc acctcaacta tttgactatt atatcaaaac
     5041 agctgccgta ttgacaaatt atcggaaatt agaccgggag cttgaatatg ctataataaa
     5101 gtaaagtgat gggttaaagt gaggttttgc ttatgggctt tagatttaga aagagcataa
     5161 aattagctcc cggtgtgagg ctaaatctag ggaaaaagag cgctagtatc agctttggca
     5221 cgaggggctt gaggcacacc ataagcacta gcggcaggag gaccgtatcc atcggcgtgc
     5281 cgggtaccgg tatctattac acaaaaagca gcaaagttgg cagcaagctt ggcagcaaat
     5341 ctgcagccag ggacgatagt gcagacccct atcaggaagt agcagagttt gaggaggctc
     5401 ttgaatacat aacatccctt cacgaaaaat gtgattatga gtacgactgg gaagcaatag
     5461 caaaagagcc gccccctttt aaggcgggag ataaggggcc aaatgaatta tccgctttgg
     5521 agaaattgga aagctttaag cctagtattt taggaagagt ttttaagtct ttggaagcga
     5581 agcgaagggc agaactcgaa gaagaagtta taaaagccgt agaagaggat aagaagctgt
     5641 atgaggcctg ggaaaaacaa aggaggctag ccgaatcggt attgaaccgc gatacaagag
     5701 cctacggaac cctgctaaaa gacataaagt tttgcagtga actggcaggc cttgtcaact
     5761 cctttagctt tcaaatcccc gatgcagact ccatgataat tgactgtcat atcaacctgg
     5821 aagatgtaat cccaactcac tataagaccc tgactaagac cggcaggctc tctattagaa
     5881 aatatagtaa gactgactac catgctattt cgaaactgta cctaacaagt ctcatgtggc
     5941 gcattgccag aaatatattg ggccttttgc cgttacaatc agtgactata aacatcctga
     6001 cctgcgtagt agataccagc ttggggcagg ttatgaacat aacgacttta tcggtaaaaa
     6061 tagacaaaag catcttcgac aaattgaact ttgacttgat tgaccccttt gatgctttag
     6121 tgaactttga gcacaatgtg aggtttttga agacaaaggg ctttcaagca gtaaaaaaat
     6181 taaacgatta gactaaaggg tggaagttat tgacatgtta cttggagctt ttatatccat
     6241 ttcaaaaggc tatgagcacg ctttaaaaga ggcactttct attggggcaa acaccatgca
     6301 gtttttcact cgaaatcctc ggggcagtgc tgccaaagcc ttagatgctg ttgatataat
     6361 gaagatgaaa gcgcttataa aggaaacaag ttttggcccc atagtagccc acgcccctta
     6421 cacattaaat cttgcctctg ggaaaaaaga ggcaagggaa tttagcatga gggtcttgag
     6481 ggaggatttg gagcggatgg aagtcgtagg agctctctac attgtgctcc atcccggcag
     6541 ccacgtaggt gccggcgtgg atagggggat acgaaaaata gcttccgctt taaaagaatt
     6601 tgcatgtggt cgtgaaaaag tgatggtgct tcttgaaaca atgtcaggtt ccggcagcga
     6661 agtgggatat actttcgagc aattgtatga aatcatggat ttgacaggga atcccgatgc
     6721 ctttggagtg tgttttgaca cctgccacgt cctaggagcc ggatacgaca tagttgaaag
     6781 cctggatgag gtgctttttg attttgataa aatcttgggg ctttccaagt taaaggtagt
     6841 ccatctcaat gacagcaagt ttcctttggg gagtcgtaag gacaggcacg caagcctggg
     6901 agagggagag ctgggactag atgcaataaa aagaatcata acacatccca aattacagga
     6961 caagcctttt ctgttggaga ctccaggtgg tattgaaaat tacaaaaagg aaataaagat
     7021 gctaagggca gttgctgaaa attaaagcgg ttaaagctct caaagatacg gggaaaggtc
     7081 ctgagggtga acctttagag acctttcctt tttattattg aattgaatta tttattatcg
     7141 tttttaccta ttaaacatat tgacaaatat ttgtcaatgt agtatcatct aactaggatt
     7201 gtgaaaatag taacaaacat aaaattataa aaagaaaaga ggagttcaaa tggtaaagat
     7261 tctttttgaa agagaccttt gccttggttg caagacctgc gaactggcct gtgcggcagc
     7321 tcattcaaag acgaaaagta tagctggcgc cataagggaa aacaagattt ctcgaatcag
     7381 aataaaaacc aaaggcggca aattgaagtt ggaacaatgc atgctctgca aaaatcctcg
     7441 ctgtattagt gtatgtccga caagagccct gtcaaaggac gaggaaagcg gaattattct
     7501 agtcgatgag atgtcatgta ccggctgcgg cctgtgcgaa gatgcatgtc cttttggtgc
     7561 tatagaatta catacatttc ccaccatctg cgacagatgt gcccattcag aagaacccct
     7621 gtgtgcctta tcgtgtccca caagagcatt aaaagttaag atagtataaa aaacatgagg
     7681 tgattttatg tctggagact tcaagtgtct gagctgtgat gcagcaaact acaagttaat
     7741 ggaaaaggcg aaaaatgaaa acattgagac cgtttgggat agggctgaag ccatgcaacc
     7801 ccagtgcggc tttggagaga tgggattgtg ctgtaccaat tgctccatgg gcccctgtcg
     7861 catagtaaag gtcagcgaag acggtcctaa gaaaggcatt tgcggtgctg acgaagacac
     7921 catagcggcc agaaaccttg ccagggctat agcagcagga tcagcggccc actcggacca
     7981 cggtcgagat attgcccatg ccctgcacta tgctgcgaaa aatcccgaat caggttacga
     8041 ggtaaagaat tccgaaaaac tcatttcact agccaaagaa tggggtatag aaactgaaaa
     8101 tttggatata gaagatatcg cagcaaaggt atccgaagtt gccctccagg aattcggcaa
     8161 acctttcggc catcttagat ttactgagag ggcaacaaaa ggcagactgg acatttggaa
     8221 caaaatgaac gtgctgcctc gagctataga tagagacatc gtcgaagttt tgcattcaac
     8281 acacatcggc tgtgatgccg attatagaaa catcatgact gcggcgacga gagctg
//
LOCUS       DAONUO010000045         8257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6587_length_8257_cov_3.755547, whole genome shotgun sequence.
ACCESSION   DAONUO010000045 DAONUO010000000
VERSION     DAONUO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6587_length_8257_cov_3.755547"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..888)
                     /locus_tag="PK768_02630"
     CDS             complement(184..888)
                     /locus_tag="PK768_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA59898.1"
                     /translation="MEYKIMLVEDDASIAKLISSHIEKYGYKPTIVSDFDRILDVFIE
                     EKPHLVLLDINLPKYDGFYWCRKIRQVSKVPIIMISARDSEMDQVMGIESGADDYITK
                     PFYYDVVIAKIKSQLRRCYGAYVPGVSEERKIELEGLTLYPERPELHFNNNFTVLTKK
                     EAVLAETLMRMSPRAVSRDVLMEKLWDDQVFIEENTLNVNVARLRKKFQEIGVDDALE
                     TVRGYGYRLNVTWRKY"
     gene            complement(963..1898)
                     /locus_tag="PK768_02635"
     CDS             complement(963..1898)
                     /locus_tag="PK768_02635"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HQA59899.1"
                     /translation="MIISASRRTDIPAFYSDWFLNRIKEGFVLVRNPMNFRQVSQVNL
                     SPDVVDCIVFWTKNPQPMINRLSQLAGYNYYFQFTLTPYDNTIETNVPDKKCLIKTFQ
                     ELSARIGRDKVIWRYDPILLTNKFDVKHHVKWFEFLANELNGHTKKCVVSFIDMYKKT
                     ERNLKGIDLVPMSEELMKEVAHHLSLISKRYNLQLEACCEDIDLTQYGIEPGKCIDDE
                     LISKIIGKKLDIKKDPNQRLSCGCVQSIDIGAYNTCQHRCLYCYANFNNALVDKNVRL
                     HSTKSPLLYGSLEEEDKVSIRKVSSNISNQLSFLD"
     gene            2264..2656
                     /locus_tag="PK768_02640"
     CDS             2264..2656
                     /locus_tag="PK768_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HQA59900.1"
                     /translation="MIKWKDEFSIGIDEIDEQHKKIFDIANEAYDLLKDEFTIDKYDR
                     ITELLEELKNYAKFHFSFEEEYMLSIRYKGYFSHKIAHDTFVDKVNSYDLDAIDENQD
                     HYILEILEFVVNWISQHILVTDKKIVSQ"
     gene            complement(2708..2893)
                     /locus_tag="PK768_02645"
     CDS             complement(2708..2893)
                     /locus_tag="PK768_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59901.1"
                     /translation="MLWGVVVGLFVGWILAMFGVNDLLIRGVFELTGRTITDAGYYTI
                     LAFIGAIGGAINDFKQK"
     gene            complement(3081..3797)
                     /locus_tag="PK768_02650"
     CDS             complement(3081..3797)
                     /locus_tag="PK768_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA59902.1"
                     /translation="MLKVQNINVYYGRIHALKDLSIDIDQGKIVTIVGANGAGKSTLM
                     WTLAGVLKAKSGTMTFMGKPYPPNAHEAAAKGIVLVPERRRLYANLTVKENLMMGAFL
                     RKDHEGIKKDLYYVYSLFPILKERTNQYAGTLSGGEQQMTAIARGLMSRPKILLLDEP
                     SLGLSPIYTAEVFKTIQEINAQGTTILLAEQNAKKALEIAHSAYVLETGRMVLSGTGK
                     ELLSDPRVQEAYLGVRHKTI"
     gene            complement(3790..4563)
                     /locus_tag="PK768_02655"
     CDS             complement(3790..4563)
                     /locus_tag="PK768_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA59903.1"
                     /translation="MALIELKNVTKVFGGIVAVKNVSFSVEKDEIIGLIGPNGAGKTT
                     IFNLITGVYDATEGKIIFQGRDITGENPVTIVKSGIARTFQNIRLFNKLSVLENIRTV
                     LFRKADYNLANALLRTPKVVRIEKKLCDKSLEFLEVVGLTDYINNRANSLPYGLQRKL
                     EIARALALEPKLLLLDEPAAGMNPDECLDLIELITRIKKEYNLTIIMIEHHMDVVMEL
                     CPRIVVINFGEKLMEGSKEEVQNDPRVLKAYLGEEYSYA"
     gene            complement(4566..5450)
                     /locus_tag="PK768_02660"
     CDS             complement(4566..5450)
                     /locus_tag="PK768_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQA59904.1"
                     /translation="MDYILGVIAVICIETIATVGLAVFTGFTGLFSLGHAAFMAIGAY
                     TAAILTYFYNAPFILALIAGMIMSGLISIILGFPTLRAKLRGDYFAIATLGFGEAIRV
                     ILENMSITQGARGLPGIKHYTTLPVAIFFAVLVLWLTKNFIWSKYGRNAIAVREDAVA
                     AEMAGIDLFKVRMLSLIYSAMCAGLAGGLFAHYLNFIQPVMFTSILSTRLTSAVVAGG
                     MGSITGPVIAVALFIAVPELLRVANMWRLVAYGLILVLIMIYRPSGIMGYHELSFEAI
                     KNLLRWISRGKTAGRKEM"
     gene            complement(5463..6335)
                     /locus_tag="PK768_02665"
     CDS             complement(5463..6335)
                     /locus_tag="PK768_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQA59905.1"
                     /translation="MFLQTLVTGLSIGGVYALMAVGYSLVFSILNFSNFAHGTIIMLG
                     AYVGYFIISNLKLGFTFSLVGAIIGAAILAVLNVKLAYTPLINRRASSLYLMITAMGV
                     SIFLENMVYVTVGSHFYSFPEIFKQTSIKFFNSDISPLDLWALVFSFISIYGLHLFLT
                     KTKLGTAIRAASYDMTIASLNGVNMSRLISVVFILAGSFAGLAGIFLGIKYMVYPTMG
                     WITNKAYIAAVIGGLGSLPGAVIGGILLGVIETFVSVYISSTIRDVFSFSMVIIFLVF
                     LPAGLMGRHLEEKV"
     gene            complement(6435..7640)
                     /locus_tag="PK768_02670"
     CDS             complement(6435..7640)
                     /locus_tag="PK768_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA59906.1"
                     /translation="MKKRIVGLLLITAMVLSVFLSACGGQQSSPPATESEGQEQEAAE
                     PIKIGHEVALTGDASMWGQSEKNALDMEIEKINAAGGVDGRPLKLISYDNRADAVEAV
                     NVAKRLIEQDKVVAIIGPAQSGVANAISSVTEEKQVPFIGTTPTNPLVTVTEDGTVKK
                     YAFRTCFIDPFQGTVAAQFAYDKLEARNAAILYDVGSDYSSWLAKYFEEAFTEMGGKI
                     VGKEAFRSGELDYRAMLGKIKQQNPDILFIPTAQKEAALAAKQARDLGITASLMGGDN
                     WGSPDLITLGGSAIEGGYFVNLASLEDPDIQDFVKEYREKYGADPVLPNPVMAIDALY
                     MLIDAIKRAGTTDGPALAEALENTKDLKVLTGILTIDPETHNPLNKPAVIQQVKDGEF
                     IFVEKYVTK"
     gene            complement(7652..>8257)
                     /gene="dapF"
                     /locus_tag="PK768_02675"
     CDS             complement(7652..>8257)
                     /gene="dapF"
                     /locus_tag="PK768_02675"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQA59907.1"
                     /translation="CICRYASDSNIAPEEMTFETLAGIYKAKVCGCEVKVDMGSIDIS
                     KARYNQIIVLNDSELTYNFLEVGVPHCVVFLDAQKTPREELLRIGRSLRNNKTHFPRG
                     TNVNFVKILDNNHIYLVTYERGVEDLTESCGTGSAAGAIVSTVLRGMESPIRVTNLGG
                     ENKVFVELKDDLSECKISIQGRAVYVAKGIICQESTNENIK"
BASE COUNT         2392 a         1880 c         1450 g         2535 t
ORIGIN      
        1 ttttgttgga aaaataacgg tataacaaaa atacacccag aagaaagcta cttaaaaaaa
       61 taaagtaata tacattttct aatgtctcaa atccaccaag atttctataa aaaataaata
      121 gaaatagcat attgaaaaga tataataata caaatgaaat atggtcatat aggaagattt
      181 tcatcaatat ttcctccagg ttacatttag tctatatcca tatcctctta ccgtctcaag
      241 ggcatcgtca acaccaatct cttgaaactt ttttctaaga cgtgccacat ttacatttaa
      301 tgtgttttct tctatgaaga cctgatcgtc ccatagtttt tccataagca catctcggct
      361 tacagctctc ggcgacatgc gcattaatgt ttcagcaagc acagcctcct tctttgtcag
      421 cacagtaaaa ttgttgttaa aatgcagttc tggcctttca gggtacaagg taaggccttc
      481 aagttcaatt tttctttctt cagatacgcc aggaacatat gcaccatagc agcgcctcaa
      541 ctgacttttt atttttgcaa taactacatc gtaatagaaa ggttttgtta tataatcatc
      601 cgcaccgctt tcaattccca tcacctgatc catttcgctg tcacgagcag aaatcataat
      661 tatgggaacc ttagatacct gccttatctt tctacaccaa taaaagccat cgtatttggg
      721 cagattgata tcaagtaaga ctagatgggg cttctcttca ataaaaacat ccaaaatcct
      781 atcaaagtca cttacaattg tgggtttata cccatacttc tcaatatggg agctgataag
      841 ctttgctata gaggcatcat cttcaaccag cattattttg tattccatac aatcctcctt
      901 ataactagtg aaagcagcag atttctgctg cttacttctt ttaggatgta aagcggccaa
      961 agctaatcca aaaaacttaa ttgattactt atatttgaag atacctttct tatgctgacc
     1021 ttgtcctctt cttccaagga tccatacaaa agtggagatt ttgtgctatg gagacggacg
     1081 tttttgtcaa caagagcatt attaaaattt gcatagcagt ataaacatct atgctgacat
     1141 gtgttatagg caccgatatc gatgctctgt acacatccgc agcttaacct ttggttggga
     1201 tcctttttta tatcaagttt tttacctatt attttcgata ttagctcatc gtcaatgcac
     1261 tttccgggct caattccgta ttgagttaaa tctatgtcct cacagcaggc ctcaagctgc
     1321 aaattgtacc tttttgagat aagggatagg tgatgggcaa cttccttcat tagttcttcg
     1381 ctcatcggca caaggtctat tccctttaag tttctttcag tttttttata catatctatg
     1441 aaacttacca cacacttttt tgtatggcca ttgagctcat ttgccaggaa ttcgaaccat
     1501 tttacatggt gttttacatc aaacttatta gttaagagga tggggtcgta cctccatatc
     1561 actttatctc ttccaattct ggcagataat tcttgaaaag tctttattaa acatttctta
     1621 tccgggacat tggtctcaat agtattatca tatggagtca aggtaaactg gaagtaataa
     1681 ttatatcctg ctaattgaga caacctattt atcataggct ggggattttt tgtccaaaag
     1741 acaatgcagt ctacaacatc aggtgacaag tttacttggc tcacctgacg aaagttcatt
     1801 ggatttctga ctaaaacaaa tccttcttta attctattta aaaaccaatc agagtaaaag
     1861 gccggtatgt ctgtccttct gcttgcactg ataatcataa tctctacttc ctaatattcg
     1921 attattgtca acagatactt ttttggcaaa caaaatcata ttaaatcctc tttacagcag
     1981 ttcactatac ttctcaatta gtctaaaagg ctttgcacaa aaattatatc agaaatcatg
     2041 gcttttatgt atcaatctta tttctaaatt aatatctctt aatgactgag catccgctgc
     2101 acggcaatta aaatcgaaat cattaccaat tctgtgaata tttctctact ttgcgacatt
     2161 tctgccgatt ttgcgagata taatgtaaaa ataggctgtt ttttgtagat gacaaccctg
     2221 ccacgttatg gtaatataag aaaaagtttg ggaggtgttg ttgttgatta agtggaaaga
     2281 cgaatttagt ataggtatag atgagatcga tgaacagcac aaaaaaatat ttgacatagc
     2341 caacgaagca tatgacctgt taaaagatga gtttactatt gataagtacg ataggatcac
     2401 cgaattgttg gaggagctta aaaactatgc taagttccac ttttccttcg aagaagaata
     2461 catgctgagt ataagataca aaggatattt ttcccacaaa atcgcacatg acacttttgt
     2521 ggacaaggtt aattcttatg atttagatgc aattgatgaa aaccaggacc attatatctt
     2581 ggaaattttg gagtttgtag tcaattggat tagtcagcac atcttagtaa ctgacaagaa
     2641 aatagtctct caatagtgtt atgaataata agggggctga tgtgtcgtaa tagatatcag
     2701 ccccactcta tttttgcttg aaatcattta tagcgccgcc tattgcgcct ataaaagcta
     2761 aaatagtata gtatcccgca tcagtgatag ttcttccggt caactcaaaa actcctcgta
     2821 ttaagagatc atttacacca aacatcgcta aaatccagcc aacgaacaaa ccaactacta
     2881 ctccccagag cacaaaaaca cctccatcgt cggactacat cgctttacac taattatatc
     2941 atattccaat agttattcac ggtgactctt aagtgcctta acaacgtcga aacttggatc
     3001 agtaattatt tccttgttaa cttgtaaacg aacaaaaagt tactaaaata aatagccccg
     3061 gttgaaccgg agcatacatg ctatatagtc ttatgtctga cccccaagta ggcctcttga
     3121 actcttgggt ctgacagcag ctctttcccc gtacccgaca ggaccatgcg ccctgtttca
     3181 aggacataag cactgtgggc aatctccaaa gcttttttgg cattttgctc agctagaaga
     3241 attgtagtac cttgagcgtt tatttcctgt atggttttaa acacttctgc ggtataaatt
     3301 ggagaaagac ccagggacgg ttcatctaac aagagtattt taggtcttga catcagaccc
     3361 cgggctatag cagtcatctg ctgttcacca ccgcttaaag tcccagcata ttgatttgtc
     3421 cgctctttta aaattggaaa gagtgaataa acataataca gatctttttt tattccctca
     3481 tggtcttttc ttagaaaggc tcccatcatt aggttttcct tcaccgtcaa attagcgtaa
     3541 agtctgcgac gttcaggcac caggacaatg cctttagccg cagcttcatg tgcattggga
     3601 gggtacggtt tgcccataaa tgtcattgtc ccgctttttg ctttgagcac acctgctaat
     3661 gtccacataa gcgttgactt gcctgctcca ttggcaccca ctattgtaac tatcttgcct
     3721 tgatcgatgt caatgctcaa gtcttttaga gcatgaattc tcccataata tacgtttata
     3781 ttctggactt taagcatagc tatactcctc ccctaagtaa gcctttagca ccctggggtc
     3841 attttgcact tcttctttac tgccttccat gagtttctcg ccaaagttta tcactactat
     3901 ccgaggacaa agttccatta ccacgtccat gtggtgctcg atcatgatga tggtcaaatt
     3961 gtactctttc tttatcctcg taattagttc tatcaagtcc aaacactcat cagggttcat
     4021 tcctgcggcg ggctcgtcta gaaggagcag ttttggctcc aatgcaaggg ccctagctat
     4081 ttccaacttt cgctggagac cgtaaggcag gctgtttgcc ctattattaa tataatctgt
     4141 aagccctaca acttccagga attctagtga tttgtcgcag agttttttct ctatcctgac
     4201 tactttcgga gtcctcaaca atgcatttgc aagattgtag tctgctttcc gaaataagac
     4261 cgtccttata ttttcgagga cgctgagttt attaaagagc cttatatttt gaaaggtcct
     4321 ggcgatgccg cttttcacta ttgtaaccgg attctctcca gttatgtccc tgccttgaaa
     4381 gattattttg ccctcagtgg catcgtatac tccggttata aggttgaaaa tcgtagtttt
     4441 acctgcgcca ttcggaccga ttaacccaat aatttcatcc ttttccacgg aaaaactcac
     4501 gttttttacc gccacgatcc ctccaaagac cttggtcaca ttttttagtt caattagcgc
     4561 cataactaca tctccttcct accggcggtc ttaccacgcg aaatccatct taacaaattc
     4621 tttatagctt caaatgaaag ctcatgataa cccattatac ctgatggtct gtatatcatt
     4681 ataagtacca atataaggcc gtaggctact aaacgccaca tattggcaac ccgcagcagt
     4741 tcaggaactg ctataaacaa cgcaacagca ataacgggtc ccgtgatgct tcccatacct
     4801 ccggcaacca ccgcagatgt tagcctggtg gataatatcg aagtaaacat tacaggttgt
     4861 ataaaattca gataatgtgc aaaaaggcca cccgcaaggc cggcgcacat cgcactgtaa
     4921 attagagaaa gcattctcac tttgaacaaa tctattcctg ccatttccgc tgctactgca
     4981 tcttccctta cagctattgc gtttctacca tatttagacc atataaaatt tttagtgagc
     5041 cacaaaacca aaaccgcaaa gaaaatggca actggcaaag tggtataatg ctttattccg
     5101 ggcaaacccc tagctccttg agttatgctc atgttttcca aaataaccct tatggcttct
     5161 ccaaagccca atgttgctat ggcaaaataa tctccgcgaa gctttgccct caaagtcgga
     5221 aagcctaaaa ttatgctgat aagccccgac attatcatgc ctgctattag agctaggata
     5281 aagggtgcgt tgtaaaaata cgtaagtatt gccgcagtgt atgcacctat cgccataaag
     5341 gctgcatgac caagggagaa tagacccgta aatcccgtga aaactgcaag gccgacggta
     5401 gcaattgttt ctatacatat aacggcaatt actcctaaaa tataatccat aattttccct
     5461 ccctagactt tttcctccag atgtcggccc atcagacctg ccggcaaaaa cacaaggaaa
     5521 attataacca tggaaaaact aaacacgtct ctgatagtcg atgaaatata tacagaaaca
     5581 aaggtttcta ttacccccaa taaaatacct ccgattaccg ctccgggaag gcttcccaat
     5641 ccaccaataa ccgccgcgat atatgcttta ttcgttatcc aacccatagt tggatagacc
     5701 atgtatttga ttcctaaaaa aatcccagca aggcctgcaa aactgccggc taaaataaaa
     5761 acaacgctaa ttagcctgga catattcaca ccatttaaag acgctatggt catgtcataa
     5821 gaagccgctc ttatggcagt gcctagtttt gtctttgtaa gaaaaaggtg aaggccatat
     5881 atgcttataa aagaaaagac caaggcccac aggtccagcg gacttatgtc actgttaaaa
     5941 aacttaatag atgtttgctt aaatatctcc ggaaaggagt aaaagtgaga acccaccgtt
     6001 acatatacca tgttttccaa aaaaatggat acgcccatag ctgtaatcat caggtaaagt
     6061 gaagatgctc gtctgttaat tagcggtgta taagccagct tgacgttcaa aaccgcaaga
     6121 atggcagctc ctataatagc tcccaccagc gaaaaggtga atccaagttt gagattagaa
     6181 ataataaaat atcctacata tgcacctagc atgataatag taccgtgagc aaaattactg
     6241 aagttaagta tggaaaacac cagcgaataa cctaccgcca ttagggcata aaccccacct
     6301 atggacaatc ctgttaccaa tgtctgcaaa aacatagttt tgtctccttt ctctagctgg
     6361 tgcaaggcat agcttattgg cagggaaaag caaaaggctt tcccctgcct ttgccttgaa
     6421 tagtaaattt cgtattactt tgtcacatat ttttctacaa agataaattc tccgtcttta
     6481 acctgttgaa ttacggccgg tttgttgagg ggattatggg tctcagggtc tattgtcagg
     6541 atgccggtca gtaccttaag atcttttgta ttttccaggg cttcggccaa ggctggtcca
     6601 tcagtcgtgc cggccctctt aatcgcatcg atgagcatat acagggcatc gatggccata
     6661 accggattag gcagaaccgg gtctgcaccg tatttctccc tgtattcttt tacaaagtcc
     6721 tggatatccg ggtcttccaa ggaagccaag ttgacaaagt acccgccttc gattgcagaa
     6781 ccacctagtg taataaggtc cgggctgccc cagttatctc cgcccattaa actagccgtg
     6841 atgcccaagt ctctagcttg ttttgctgcc agagccgctt ctttctgagc ggtgggaata
     6901 aataaaatat cgggattctg ttgttttatc tttccaagca ttgccctgta gtccagctct
     6961 cctgaacgga aagcctcttt gcccactatt ttgccaccca tttcagtaaa tgcttcttca
     7021 aagtacttgg caagccatga agagtagtct gatcctacat catataatat ggcagcattt
     7081 cttgcttcca gcttatcata agcaaactga gcagctactg tgccctggaa ggggtcaata
     7141 aagcaggtcc taaaggcata ttttttcact gtaccatctt cagtcacagt gaccaatggg
     7201 ttagtgggag tagtccctat aaagggtacc tgcttttctt ctgtaactga agaaatcgca
     7261 tttgctacac cgctttgggc ggggccaatg atagccacta ctttgtcctg ctcgattaat
     7321 ctttttgcaa cgttaaccgc ttcgaccgca tccgctcggt tatcgtaaga aatgagcttt
     7381 aatggcctgc cgtcaactcc tccggcagca ttgatctttt ctatttccat atccagcgca
     7441 ttcttttccg attgacccca catcgatgca tcgccagtta aagcaacttc atgacctatt
     7501 ttaataggtt cggctgcctc ttgttcctgt ccttcagact ctgttgcagg aggacttgat
     7561 tgttgtccac cgcaagcact caaaaagacg ctcaacacca ttgccgtaat tagcagtaag
     7621 ccaactattc ttttcttcac gctctctccc cctattttat attttcgtta gtactttcct
     7681 ggcatataat ccctttggcc acatacacgg cccttccctg gatgcttatc ttgcattcgg
     7741 ataagtcatc ttttaattct acaaatacct tgttctcacc ccccagatta gtcactctta
     7801 tcgggctttc catgccgcgt aatactgtag atactatggc gcctgctgct gaaccggtgc
     7861 cgcaagattc tgtcaaatct tcaacgcctc tttcataagt aaccaaataa atatgattat
     7921 tatctaagat ttttacaaaa ttaacattcg tccccctggg aaaatgggtt ttgttatttc
     7981 tcaacgacct gcctatcctc agcagttctt ccctcggagt tttctgtgca tctaagaaaa
     8041 ctacacaatg gggaactcct acttctaaga agttatatgt caattcgcta tcgtttaaaa
     8101 ctattatctg attatatctg gctttgctta tatctattga acccatatca acctttactt
     8161 cacaaccgca gacctttgct ttataaatac ccgctaaagt ttcaaaagtc atctcttctg
     8221 gtgctatatt gctatcgcta gcatatcggc atataca
//
LOCUS       DAONUO010000046         8241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6604_length_8241_cov_3.689348, whole genome shotgun sequence.
ACCESSION   DAONUO010000046 DAONUO010000000
VERSION     DAONUO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6604_length_8241_cov_3.689348"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PK768_02680"
     CDS             <1..472
                     /locus_tag="PK768_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA59908.1"
                     /translation="EITNTMSIMLSTTISKVNEEFNKVFTELFEGGRAELIVSGEGSV
                     LDCGIEINAQPPGKKLQNISLLSGGERALTAIALLFAILNTKATPFCVLDEIEAALDD
                     VNITRFTRYLKEVSKHTQFILITHRKATMEVADALYGISMEDAAVSKVLAVKMV"
     gene            492..1388
                     /gene="ftsY"
                     /locus_tag="PK768_02685"
     CDS             492..1388
                     /gene="ftsY"
                     /locus_tag="PK768_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQA59909.1"
                     /translation="MSFLNRLKESLSKTRENFSERIDSLLKFTKKIDDETFEELEELL
                     IASDVGVNTTLELIDKLKEESRKIKSPEELKEKLKEEIINLFPESSNISLTSPTVMLV
                     VGVNGVGKTTSIGKLANKYKKQGKKVILAAADTFRAAAIDQLEIWGNRVGVDVIKHQE
                     GADPASVLFDALQAGKARKADLIICDTAGRLHTKKNLMKELKKLYRVCQKEYPEANVT
                     SLLVLDATTGQNALVQAKTFKEAVDFQGIILTKLDGTAKGGIVVAIASELKIPVWYIG
                     IGEGIDDLQEFDHQTFVDALFA"
     gene            1814..2173
                     /locus_tag="PK768_02690"
     CDS             1814..2173
                     /locus_tag="PK768_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HQA59910.1"
                     /translation="MDNITKLNLLFDYYGDFLTKKQRDIFEQYYINDLSLGEIAENSG
                     ITRQGVHDILRRAERTLENFEKKLDMVNKYLSYRKKIEKILVLLEEIEPGVAAEYKQK
                     FTDIYNGVATLLKEGGV"
     gene            2166..3512
                     /gene="ffh"
                     /locus_tag="PK768_02695"
     CDS             2166..3512
                     /gene="ffh"
                     /locus_tag="PK768_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQA59911.1"
                     /translation="MCRLAFESLVQKLGDIFKKLRGKGKLSEQDVKQAMREVRLALLE
                     ADVNFKVVKDLVSKITDRAVGQEVMESLTPAQQVIKIVNEELTELMGSKQSKLNFSRD
                     ITVIMLVGLQGSGKTTTAGKLGKLLSKDGKRPLLVAADIYRPAAITQLQVVGEKLNLP
                     VFTMGQQNPVDISKAALEHSRKHNYNVVIIDTAGRLHIDDELMMELTNISQAVKPSEI
                     LLVVDSMTGQDAVNVAEEFNRRLDLTGLILTKLDGDSRGGAALSVKAVTGCPIKFVGM
                     GEKLDDLEVFHPDRMASRILGMGDVLTFIEKATQDLDLQKAKELEKKLKSQNFDLNDF
                     LEQLLQVKKIGSFDQILNMIPGLSAQKLKGVEFDEKELVRVEAIINSMTPEERSNPSI
                     INGSRRKRIAAGSGCSIQDVNRLLKQYNQTRKMIKQFAGFEKSRKKGKHRFPFFGF"
     gene            3527..3814
                     /gene="rpsP"
                     /locus_tag="PK768_02700"
     CDS             3527..3814
                     /gene="rpsP"
                     /locus_tag="PK768_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQA59912.1"
                     /translation="MAIKIRLKRMGAKKKPFYRVVVADSRMPRDGRFIEEIGYYNPLT
                     DPIEVKINEEKASDWLKKGAIPTDTVKYLFKNLGILPDNKKTEEQEIKSEE"
     gene            3858..4085
                     /locus_tag="PK768_02705"
     CDS             3858..4085
                     /locus_tag="PK768_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HQA59913.1"
                     /translation="MLELVEYIAKALVDHPENVTVNQVEGEQSIILELKVDPEDMGKV
                     IGKQGRIAKAIRTVVKAAAAKEGKRVVVEII"
     gene            4148..4666
                     /gene="rimM"
                     /locus_tag="PK768_02710"
     CDS             4148..4666
                     /gene="rimM"
                     /locus_tag="PK768_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778270.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQA59914.1"
                     /translation="MSYRNKHLTVGKVLCPWGVGGQVKVEPRTDNPKRFRDLPGVFIE
                     FKDSTVSYNIESVIFVNDYYPVLKFEGINSRQEAETLRGCYLKIPRKYAVRLPEDHFF
                     ICDIIGLSVFDESDTYIGIVKDVLHTGANDVYVVEAQDKKEVLIPAIKQVVKNIDLEN
                     DKMVIRPLEGML"
     gene            4666..5385
                     /gene="trmD"
                     /locus_tag="PK768_02715"
     CDS             4666..5385
                     /gene="trmD"
                     /locus_tag="PK768_02715"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQA59915.1"
                     /translation="MLIIHVLTLFPEFFESPFNVSIIKRALDKGLIQIELINIRDFSR
                     DKHKKVDDYPYGGGSGMVMKPEPIFEAVDYVEKTIEGERRRIILMSPQGKTFNQSLAR
                     ELSTEKNLVFICGHYEGIDERVKTLITDEISIGDYILTGGEIPALAIIDAVTRLVPGV
                     LGSSQSLEEESFSDGLLEYPQYTRPEVYRGLKVPDVLLSGNHKEIELFRRREALIRTR
                     EKRPDLFKKLKLTDFDKELLQ"
     gene            5536..5877
                     /gene="rplS"
                     /locus_tag="PK768_02720"
     CDS             5536..5877
                     /gene="rplS"
                     /locus_tag="PK768_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQA59916.1"
                     /translation="MDMITLVEQQQMKKDIPDFRPGDTVSVHTKVVEGSRERIQVFEG
                     TVIARKGSGLKETFTVRKISYGVGVERVFPVHSPRIDKIEVVKKGKVRRAKLYYMRKL
                     RGKAARIKERD"
     gene            5914..6774
                     /gene="ylqF"
                     /locus_tag="PK768_02725"
     CDS             5914..6774
                     /gene="ylqF"
                     /locus_tag="PK768_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HQA59917.1"
                     /translation="MDIQWYPGHMVKTKKMIKQNLRLVDVVLELVDARAPLSTKLPDA
                     DSLVGSREIITVLNKADLADKKVTEMWINYFKDRGIPAVSVDTLRKTGFKRLFSLINS
                     VKKRSLLGSRRCMIIGVPNVGKSSLMNQMAGRKSARTADVPGVTRSKMWLKVSDNLEL
                     LDTPGILWPKFEDKAVGIKLAMLGSIKEDLLDLQQLAFILIKFLSIYYPERLKSRYNL
                     NTNIPPLQALEKIAASRGFFLEGGILDIERSAKTIITEFRQGKIGAISLERPEEKEGF
                     WIDISNQDIV"
     gene            6791..7423
                     /locus_tag="PK768_02730"
     CDS             6791..7423
                     /locus_tag="PK768_02730"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQA59918.1"
                     /translation="MLIENELVRLQKLYDYERKLMEKGYTLVAGVDEAGRGPLAGPVV
                     ASAVILEVDTQIIGLRDSKKISEKKREGVYEDIKSKAIAFAYDVVDVQYIDDHNILNA
                     TLMAMKNAVCKLPVKPEYILVDALRIPDIDIPQESIVHGDSVCACIAAASVIAKVERD
                     KIMRSYDLLYPQYGFCKHKGYGTKEHINSIKKHGFCPIHRKSFSIRGLNI"
     gene            7424..7777
                     /locus_tag="PK768_02735"
     CDS             7424..7777
                     /locus_tag="PK768_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQA59919.1"
                     /translation="MNKKELGAIGEKYAADYLKGKKYEILQLNFSCPLGEIDIIAKYK
                     NSIVFVEVKTRTSTKFGKGMEAVNYYKQQKLRKVALYYLNNKAPFFSNIRFDVIDILI
                     QNEDEIKIEHIENAF"
     gene            7789..>8241
                     /locus_tag="PK768_02740"
     CDS             7789..>8241
                     /locus_tag="PK768_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQA59920.1"
                     /translation="MLAQVKSCSIYGIESFLVDVEVDISSGLPDFHIVGLPDVAVKES
                     RERVRAAIKNQSYVFPIRRITVNLAPADIRKEGPHLDLPIALGILAATEQIPVEILKD
                     YAIIGELSLEGKVRPVNGVLPMAIEIKESGLKGVMVPIENVEEASVIKG"
BASE COUNT         2842 a         1311 c         1886 g         2202 t
ORIGIN      
        1 agagatcact aataccatgt caataatgct gtctaccact ataagcaaag tcaatgagga
       61 gtttaataag gtattcacag aactttttga aggcggcaga gcagaactaa ttgtatccgg
      121 tgaaggcagc gtattggact gcggaataga aataaacgct caaccaccag gaaaaaaatt
      181 acaaaatatc tctcttttat cagggggaga aagggcattg acggctattg ctttactttt
      241 tgcaatttta aatacaaaag caacaccttt ctgcgtcctg gatgaaatcg aagcagcttt
      301 ggacgatgta aacataaccc gctttacacg atatctcaaa gaagtctcga aacatactca
      361 atttatatta attacacata gaaaagctac catggaagta gcggacgctt tatatggcat
      421 atctatggaa gatgctgcag tgtcaaaagt tttggcagta aaaatggtat aataaaaacg
      481 aggtgaagta aatgagcttc ttaaacaggt taaaagaaag tttaagcaaa actagggaaa
      541 acttcagcga gaggattgac tctctgttaa aattcacaaa aaagatagat gatgagacat
      601 ttgaggagct tgaagaactt ttaatagcat ccgatgttgg cgtaaataca actttagaac
      661 taatagacaa gcttaaagaa gaatctagaa aaataaaaag tcccgaagaa ctgaaagaaa
      721 aactaaaaga ggagatcata aatcttttcc ctgaaagcag caacatatca ttaacttccc
      781 caacagtcat gttagtagtt ggtgtgaatg gagtaggcaa aaccacttcc ataggcaaac
      841 tcgccaacaa atataaaaaa cagggcaaaa aggtcatatt agccgcagca gataccttta
      901 gggcagcggc tattgaccaa ctcgagattt ggggcaatcg tgtaggtgtc gacgtaataa
      961 aacatcaaga aggcgcagac cctgcatcgg tgctcttcga tgcactgcaa gccggaaaag
     1021 ccagaaaggc tgacttgata atctgcgata cagcaggcag gcttcacacg aaaaagaatc
     1081 tgatgaaaga actgaaaaag ctttacaggg tttgtcaaaa ggaatatcca gaagctaatg
     1141 tcacaagcct attggtttta gatgccacta caggtcagaa tgccttggtc caagcaaaga
     1201 cctttaaaga agcggtggat tttcagggaa ttatccttac gaagctagac ggcacagcaa
     1261 aaggcggaat tgttgttgcc atagcgtctg aacttaaaat acccgtatgg tatataggta
     1321 tcggcgaagg aatagatgat ttacaagaat ttgatcacca aacttttgtt gatgctttat
     1381 ttgcctaata attttaagac tattttacta ctactatgaa acatattacg cgaaacgatt
     1441 ataaggttaa agatgaacgg cacctctacc taaagccata cctgttattt taaaagcttg
     1501 gtcctgcaca acgcagggtt tcacgggcaa ttcggtatcc tgccgacaac ctgccagcgt
     1561 gcatacaaca cgatgaccct gcttcacata tccatggtta aaaaaaacaa tggtctggct
     1621 aaaggtattg tttgccgtgc atcttaaacc tcaagttaca gtatattgtc aattagaatc
     1681 aacacaatgt ggggatgttg cctcaataag gattgattta tttcgaagaa tttatcgcaa
     1741 aacagttgac ttcgtgcaat tctttatgta aaataaacag gtgtaaagga taaatgcttt
     1801 acaggtgaat taagtggata acattacaaa actcaacctg ttgtttgatt attacggtga
     1861 ttttttgaca aaaaagcaaa gggatatctt cgaacagtat tatattaatg atttgtcatt
     1921 gggcgaaatt gctgaaaaca gtggtataac caggcaaggt gtccatgata ttcttcggcg
     1981 tgccgagagg acattggaaa actttgagaa aaagttggat atggtaaaca agtatctatc
     2041 ttatagaaag aaaatcgaga agatacttgt gcttcttgag gaaatcgagc ctggtgttgc
     2101 tgccgagtac aagcaaaagt ttactgatat ttataatggt gttgccacgt tgctcaagga
     2161 aggtggtgtg tagattggcc tttgaatccc ttgtacagaa attgggagat atctttaaaa
     2221 agcttcgcgg aaagggaaaa ctgtcggagc aggatgtaaa gcaggctatg agagaggtaa
     2281 ggcttgcact tttggaagct gatgtgaact tcaaggttgt aaaagacttg gtctcaaaaa
     2341 ttactgatcg agcagtgggt caggaagtta tggagagctt gactcctgca cagcaggtca
     2401 taaaaatagt caatgaagaa ttaaccgaac tgatgggctc taaacaaagc aagcttaact
     2461 tctcaagaga catcaccgta attatgctag taggtcttca aggttcagga aaaacaacga
     2521 ctgccggcaa gctaggaaaa ttgttatcaa aagatgggaa aagaccccta ttagtggctg
     2581 cagacatata ccgtcctgcg gcaataacgc agcttcaagt ggttggagaa aagctaaatt
     2641 tgcctgtttt tacgatgggt cagcaaaatc ccgttgatat ttcaaaagct gctctagagc
     2701 attcaaggaa acataattac aatgtggtaa taatcgacac ggcgggtcgt ttgcatatag
     2761 atgacgaatt aatgatggaa ttgactaata taagccaagc agtaaaacca agtgaaatac
     2821 tgcttgtcgt ggatagtatg acgggtcagg atgcggtaaa cgtagcagag gagtttaaca
     2881 gaaggcttga ccttacaggg ttaatattga caaaattaga tggagacagt cgtggtggtg
     2941 ctgcactttc cgttaaagct gttacaggtt gccccattaa atttgtaggt atgggtgaaa
     3001 aattagacga tttagaggtt tttcatcctg atagaatggc ctctagaata cttggcatgg
     3061 gggatgtttt aaccttcata gaaaaggcga ctcaagatct agatttgcag aaagctaagg
     3121 agctagagaa gaagctaaaa agccagaact tcgacttgaa tgatttttta gagcaactgt
     3181 tacaagttaa aaaaattggt tctttcgatc agattcttaa catgataccc ggactttcag
     3241 ctcaaaaatt aaaaggcgtg gaatttgacg aaaaggaact tgttcgagtt gaggccatta
     3301 taaattcaat gactcctgaa gagaggagta atccttcaat tattaatggc agtcgccgta
     3361 agcgcatagc tgcaggcagc ggttgttcca tacaagatgt aaaccgactg cttaaacaat
     3421 acaatcagac tagaaaaatg ataaaacaat tcgcaggctt tgaaaaaagc cgtaaaaaag
     3481 gcaaacatag attcccattt tttggatttt agaaggaggt gaaaaaatgg ctataaaaat
     3541 acgattaaag cgaatgggtg caaagaaaaa acctttttat agagttgtag tggctgatag
     3601 ccgtatgcca agagatggaa ggttcatcga agagattggt tattacaacc ctttaacaga
     3661 tccgatcgaa gttaaaatta atgaagagaa ggccagtgat tggttgaaaa aaggtgccat
     3721 tcccaccgat accgttaagt acttatttaa aaatttaggg atcttgcctg acaataaaaa
     3781 aacagaagaa caagagatta aaagtgaaga ataattcata gtagataata attgtaactt
     3841 taaaggagga agtgtcagtg ttagaattag tggaatacat tgcaaaagca ttggtagatc
     3901 atccagaaaa tgtaactgtc aaccaggtgg aaggtgagca atccatcatt ttagaactaa
     3961 aggtagaccc agaagatatg ggaaaagtaa ttggaaaaca aggaagaatt gccaaggcaa
     4021 ttcgcacagt ggttaaggca gcagctgcca aggaaggcaa aagagttgtc gtagaaatca
     4081 tatagtttca tgggttgggg taacctgacc cttcaataaa tctcgtttat taaaaggaga
     4141 aagtggcttg agttatagaa acaagcatct tacggtaggt aaagttttat gtccttgggg
     4201 agttggaggc caggttaaag ttgaacctcg aaccgataac cctaagagat ttcgtgactt
     4261 acctggagtt ttcattgaat ttaaagatag tactgtttcc tataatatcg aatcagttat
     4321 ttttgtaaat gattattatc ccgtattaaa atttgaaggt attaattcaa ggcaagaagc
     4381 agagacttta agagggtgct atcttaaaat tccaagaaaa tatgccgtaa gactgccaga
     4441 agaccatttc tttatatgtg acattatagg gcttagcgtc tttgatgagt ctgacacata
     4501 tataggaata gtcaaagacg tgctgcatac cggggccaat gacgtttacg tagtggaggc
     4561 acaggataag aaggaagtcc taattccagc aattaagcag gttgtaaaga atatagattt
     4621 ggaaaatgat aaaatggtga ttaggccgct ggaagggatg ctgtgatgct tataatacat
     4681 gttttgacac tttttcccga gttttttgaa agccctttta atgtcagcat tatcaagcga
     4741 gctcttgata agggtttaat acaaatcgaa ctaataaaca taagggattt ttcccgggat
     4801 aagcataaaa aagtcgatga ttatccttat ggtggcggca gcgggatggt catgaagccc
     4861 gaacctatat ttgaggcagt agattatgtt gaaaaaacaa tagagggcga aaggcgcagg
     4921 ataatactaa tgtcccccca aggtaaaact tttaatcagt ccttagcccg agagttgtct
     4981 acggaaaaga atcttgtttt tatctgcggc cattacgaag gaatagatga aagagtaaaa
     5041 actttaatta ccgatgaaat ttcaataggc gattacattc ttacgggggg cgagattcca
     5101 gccctggcca taatcgacgc cgtaacaaga cttgtgcctg gagtgttagg cagcagccag
     5161 tccttagaag aggaatcttt tagcgatggg ctactggaat atcctcaata tacaaggcca
     5221 gaagtgtata gaggccttaa agtacctgat gtgctgcttt caggcaacca taaggaaata
     5281 gagcttttca ggcgaagaga agctttaatc aggactcgtg agaaaaggcc tgatctattc
     5341 aaaaaactca agcttacaga ttttgacaaa gagttgttgc agtgagcttt ataatttata
     5401 aagacgttaa agtgaagctg taaactgcga tgtttttaac aatttatatg ttgattgtca
     5461 ataaataccc tttgtgttat aatacttttt gttgttgtcg aaacaccaaa ttaccagcag
     5521 aaggagggaa tacggatgga tatgattact cttgtagagc aacaacaaat gaaaaaagac
     5581 atacctgact ttagaccggg agataccgtt agtgtccata caaaggttgt agaaggtagt
     5641 cgagagagaa tacaggtttt cgaagggact gtaattgcta gaaaaggcag cggtttaaag
     5701 gaaaccttta ctgttcgcaa gatatcatac ggcgtaggtg ttgaaagagt atttcctgtt
     5761 cactcaccgc gcattgataa aatcgaagtt gttaagaaag gtaaagtgcg tagagctaaa
     5821 ctttactaca tgagaaaatt aagaggtaaa gctgcacgaa taaaggagag ggactgaaaa
     5881 ggtccctttt gttgttctta ggaggtggtc tcggtggata tacaatggta tccaggccat
     5941 atggttaaaa caaaaaaaat gattaaacaa aatttaaggt tggttgatgt tgtattagag
     6001 ttagtagatg ctcgggcacc ccttagcacg aaactccctg atgctgacag tttagtagga
     6061 agtagagaaa ttatcacagt gctaaacaaa gctgatttag cagataaaaa agttaccgaa
     6121 atgtggataa attatttcaa agatcgggga attcctgcgg tctcggttga tactctaagg
     6181 aagacgggct ttaaaaggct ttttagtttg attaacagcg taaagaaaag gtccttattg
     6241 gggtcaagac gatgcatgat aataggtgtt ccaaatgtgg gaaaatcatc tttaatgaac
     6301 caaatggcag ggagaaaaag tgctagaacg gctgatgtcc ctggagtaac ccgtagcaaa
     6361 atgtggctca aagtgagtga taatttggaa ttgctggata cgccggggat tttatggcca
     6421 aagtttgagg acaaggctgt tggcatcaag cttgcaatgt taggttctat taaagaagat
     6481 ttattggatt tacaacaact tgcatttata ctaataaaat ttttgtctat atattaccct
     6541 gaaaggctca agtcaaggta caacttgaat actaatatcc ctcccctgca agccttggaa
     6601 aaaatagccg caagtcgagg ttttttcctt gaaggaggaa tccttgatat tgaaagaagc
     6661 gcaaagacca taataacgga attcagacag ggtaagatag gtgcaatatc tttagaaagg
     6721 ccagaagaaa aagaaggatt ctggattgat atatcgaatc aagatattgt ctgagggagg
     6781 ggattttatt atgctaatag aaaatgagct ggttaggctg cagaagttgt acgattatga
     6841 gagaaaattg atggagaaag gctatactct ggtagctggg gtagatgaag ctggccgagg
     6901 ccctttagcc ggaccggttg tagcatccgc tgttattctg gaagtagata cacaaatcat
     6961 tggcctaagg gattcaaaaa aaataagtga aaagaaaagg gaaggagtat atgaagacat
     7021 caaaagcaag gccatagctt ttgcttacga cgtggtagat gttcagtaca tagacgatca
     7081 caacatttta aatgcaacac taatggcgat gaagaatgca gtttgcaaac tacctgtaaa
     7141 accagaatac attttggtag atgcgctaag gataccggat attgatattc ctcaagagtc
     7201 aattgtacat ggtgatagcg tttgtgcttg cattgccgct gcatctgtca tagcaaaagt
     7261 ggaaagagac aagatcatgc ggagttatga cctattgtac ccacaatatg gtttttgcaa
     7321 acataagggg tacggcacga aagaacatat taattctatt aaaaagcacg gtttttgtcc
     7381 aattcaccgc aagagctttt ccattagggg gttgaatata taattgaata agaaggaact
     7441 tggggcaatt ggggaaaagt atgccgccga ctatttaaaa ggcaagaaat acgagatatt
     7501 acaattaaac tttagctgtc ccttagggga gatagatatt atagctaaat acaaaaatag
     7561 catcgtattt gtagaagtaa aaacgagaac atcaacaaag tttggaaaag gaatggaagc
     7621 ggtaaattat tataaacagc aaaagttaag aaaagtagct ctgtattacc ttaacaataa
     7681 agctcccttc ttttccaata ttaggtttga tgtaatagat attttgatac aaaatgagga
     7741 tgaaattaag atagaacaca ttgaaaacgc cttttaaggg gtgatccaat gttagcccag
     7801 gttaaaagct gttctatata cggcattgaa agctttttag ttgatgtaga ggtggatatt
     7861 tcaagcggcc ttcctgactt tcatatagtt ggtcttcctg atgtggctgt aaaagaatct
     7921 cgtgaacgag taagagctgc cattaaaaat cagagttatg tttttcctat aaggagaatc
     7981 acagtaaatc tagccccggc agatattcgg aaagaggggc cgcatttaga tttgcccata
     8041 gctctaggaa ttttagcggc aacggaacag attcctgttg aaatattaaa agactatgct
     8101 atcatagggg agctttcatt agaaggaaaa gtaagaccgg taaatggtgt tttaccaatg
     8161 gctattgaaa taaaggaaag tggtctaaaa ggtgtaatgg tccccataga gaatgttgag
     8221 gaagcttcag taattaaagg t
//
LOCUS       DAONUO010000047         8218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6626_length_8218_cov_3.713463, whole genome shotgun sequence.
ACCESSION   DAONUO010000047 DAONUO010000000
VERSION     DAONUO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6626_length_8218_cov_3.713463"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1332)
                     /locus_tag="PK768_02745"
     CDS             complement(289..1332)
                     /locus_tag="PK768_02745"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HQA59921.1"
                     /translation="MSEQDKKIEVDGITPIICVNLPPGISPKLAKKQTSQDEEADTQQ
                     LFSGGIAYVPNLRSNNVSVVHIPTARLITNIPVGNGPFAVDLSPDKSFAYVSNFNSAT
                     LSIIRTLDNRVVDTVNLNTPPFTAVGSSGVKVTRNGRFVYVANFNSNNISVVDALQRS
                     VLTVIPLPGGSGPQNIDIRGSLAYVTLSLANQVGVIDLNANLLVKTINVGNNPADIDI
                     ATTRPLALVTNLLSGDVSIINTNIAESSPNTITIGTFPGGISFSPNGRRAYAANQASD
                     NISVIDVARHTQVLTIPVGDGPIGVKVNSSGRFVVVSNAGSNTISIIDTTINQVIETV
                     TVGTFPLYLAILD"
     gene            complement(1590..2273)
                     /locus_tag="PK768_02750"
     CDS             complement(1590..2273)
                     /locus_tag="PK768_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HQA59922.1"
                     /translation="MKYELRGDTLPVVILTLEQGESIFTETGGMSWMSDGFEMKTNME
                     GRLFGGIARTLAGESVFMTTYTMKKASGIIAFSSSFPGKIVPIHLEAGESIICQKQSF
                     LCAERSVKLSAHLKKKLGAGLFGGEGFILQKITGPGWAFLEIDGEAVEYQLEPGEKLK
                     VDTGHVAIFEPSVDFNIEMVKGFKNILFGGEGLFLTTLEGPGKVWLQSMPINNLAARL
                     IPFLPSSDD"
     gene            complement(2372..3178)
                     /locus_tag="PK768_02755"
     CDS             complement(2372..3178)
                     /locus_tag="PK768_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 family protein"
                     /protein_id="HQA59923.1"
                     /translation="MKLLIICSEKSNTQPKNSFEIKTDTRFANTKFIPHLLNKKEVCT
                     VCGKDCFWCRGRYDLDFKEDIVRIIKLPDIYEIFVDNTDDYLPESFQKHDVAIAIGIH
                     QDILVELPKLIKESGGKALIVPCESGEWVSRWTRDEVIERCKRFGLEYAFPKPFCNLS
                     YGKFPIINEFIDKFKIGKPKFKLYVDNNDTIKRAEVIISAPCGNGYNVCKHLVGKKLG
                     DEAKAAVAKFWHSYPCLGGMTIDPEVGDTILHIGGYIHYDALSNAHIVRI"
     gene            3367..3897
                     /locus_tag="PK768_02760"
     CDS             3367..3897
                     /locus_tag="PK768_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59924.1"
                     /translation="MSIAVAVSAQGYGDEGAFDYRAEMTGHIIIKPSTGQEFLDMLAD
                     LSKSRGPIFLMKVFSHSYTRGIIMTNWSGFYDEPGPEDTKKAAYLRDLADRIQEGNIR
                     FAPDSQILLFGCDLGAFSQKLSAVTGGTVVGSDGGTYPEIWGNRETGVFLTTDDWLVY
                     KNGSFAYSAGKRYRAW"
     gene            complement(4387..5028)
                     /locus_tag="PK768_02765"
     CDS             complement(4387..5028)
                     /locus_tag="PK768_02765"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HQA59925.1"
                     /translation="MKAVIFAGGQIKNYDKLKKYIEGSDLVICADSGIEHAFNMGIMP
                     DLLVGDMDSINHDTLEKANKLGIARHNFPAEKDSTDTELALDIALAKGASEAILLGGL
                     GDRPDHGLANIFLMVNFFNRGLKLKLVGENWEMFLIDKMAEVTGKKGDILSLIPLSPE
                     VAGVNTEGLYYTLKGETLYMGPTLGISNVFLGNTARVTIKQGLLLAVKYTQKG"
     gene            complement(4994..5704)
                     /locus_tag="PK768_02770"
     CDS             complement(4994..5704)
                     /locus_tag="PK768_02770"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQA59926.1"
                     /translation="MEEKEIYEYILKGKPAFEPREEYRYSNSNYVLLGIICEKATGME
                     YEELLYDKIIDKLNLQNTCILPYDETPEKLVKGYDRDVIPLGIKRIETFNDSLSSLAY
                     SAGAIVSNCNDLIEFYSSLLSGEIISDKYLDEMMTFIDCTDEDLYVQTGYGLGLRRFE
                     IGGHVLIGHTGTIPGYGGLLMYNADKDYIIAALANISYFNQIAVLTEIINYIDSREAN
                     SFIMEAYDESCYICRWSN"
     gene            complement(5605..6216)
                     /locus_tag="PK768_02775"
     CDS             complement(5605..6216)
                     /locus_tag="PK768_02775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQA59927.1"
                     /translation="MISRFKKSITIVVLLVIIFVFLHIKSHPRLSVFYEINQDENNST
                     FDAELTSILIDTLNQLEIPGMQVAIKIYGDVHNYNIGTVDYERTERVGEDNVFKIASV
                     TKIFTSTLVLKLAEEGYFNLDDTIDKWFPELINSETITIRTLLNHTSGVYSYTENFAL
                     QCKALLNPRKLWKKRRFMNIFSRGNLLLNLEKSTGIQIRIMYY"
     gene            6428..7111
                     /locus_tag="PK768_02780"
     CDS             6428..7111
                     /locus_tag="PK768_02780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQA59928.1"
                     /translation="MKTINVLYEDNHLLVVTKPPNLLSQGDKTGDPDLLSILKDDLKE
                     RHNKPGNVYLGLVHRLDRPVGGVMVFAKTSKAAARLSDQVRRNLFKKTYIAVIRGLPP
                     REKDTLIHYLLKNTKTNRVEAVKKGVKGAKEAILDYEVISYAHPYCLVKIDLHTGRPH
                     QIRVQFSKIGCPIIGDQRYGYKDKYNHIALWAYKISFEHPTLRKKVEFSALPENRPPW
                     SRFSLAKLK"
     gene            7248..7475
                     /locus_tag="PK768_02785"
     CDS             7248..7475
                     /locus_tag="PK768_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQA59929.1"
                     /translation="MKEKVQAALDKIRPALQADGGDVELVDVDEASGIVKVRLTGACG
                     GCPFATMTLKNGIEETLKQVIPEVQEVQQVF"
     gene            complement(7620..>8218)
                     /gene="cobC"
                     /locus_tag="PK768_02790"
     CDS             complement(7620..>8218)
                     /gene="cobC"
                     /locus_tag="PK768_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HQA59930.1"
                     /translation="HGETMWNRQLKYQGQSDIPLTDEGRAQARILSERLKHERIDAIY
                     ASDLERTVETAEIIAEPHGLKVLRAPLMRELNFGLWEGMTYDEIMQKWAQEYRDWQAD
                     PYNKTPPEGESLSDLCERVSEFLKEAANKHPDGRILVVSHAGPIRAILSVLLNLRQDF
                     FWKFKISNTSVTIIEYDGQKELPESDAFIVTVNNTYHL"
BASE COUNT         2385 a         1690 c         1578 g         2565 t
ORIGIN      
        1 gcagtagaag attacgacat tgacaaaata cagcaatttt tagatgacaa tccgaagcta
       61 aaagacttgt attccgccga tgacggaaaa tgcaccatct tttatttgaa agatgctaca
      121 tgcagctttt gttccttttt ctccgaaccg gaagtacaca ggttctaagg tcaacttcta
      181 catagtattg aaagaggaag gatatgtttt tgatatatcc ttccttgctt tttataacac
      241 ataatagtaa tcaaaaagtt ttttgaaacc attaactgtc tatatgaatt agtctagaat
      301 ggctaggtac aaaggaaagg tgcctacagt caccgtctct atcacctgat taatcgtggt
      361 atcgatgata gatatggtgt tgctgccggc atttgatacc accacaaatc tgccgctgct
      421 gtttaccttt accccgatgg ggccgtcacc tacgggtatg gttaatacct gcgtatggcg
      481 ggctacatct ataaccgata tattatctga tgcttggttt gcggcataag cccttctgcc
      541 attgggagaa aaagaaatcc ctccggggaa agtgccgatg gtaattgtgt tgggagacga
      601 ctctgcaata ttggtattta taatagaaac gtcaccggat aacaaattgg tcaccaatgc
      661 caaaggcctg gttgttgcta tgtcaatatc tgcagggttg tttcctacgt tgatggtttt
      721 aacgagcaga ttagcattca ggtcaattac ccccacctga tttgcaagcg aaagtgtaac
      781 atatgcaagg cttccccgga tgtcaatatt ttgaggcccg ctcccgcctg gaaggggaat
      841 cacggtgagc acgcttcttt gcaaggcatc gaccacagaa atgttgttgc tgttaaaatt
      901 agccacgtaa acaaatcttc cgtttctggt cacttttaca ccggaagaac ctaccgctgt
      961 aaagggtggg gtgtttaagt taacggtgtc taccacccta ttatcaaggg tccttattat
     1021 tgaaagagtt gcggaattaa agtttgacac atatgcaaag cttttatccg ggcttaaatc
     1081 aaccgcaaat gggccattgc ccaccggtat gtttgttatt aatctggcag tgggaatgtg
     1141 gactaccgat acattattgc ttctcagatt gggcacataa gcaattcctc cggaaaacaa
     1201 ttgttgtgtg tcagcttcct catcttggct tgtttgtttt ttggcaagct ttggacttat
     1261 gccaggaggc aaatttacac agattatcgg ggtgatacca tcaacttcta tttttttatc
     1321 ctgttcagac atgttttatc ctcctctcaa cagctaaaga attgcacaaa caaagtagta
     1381 tattacaact aacccacatt agagattatg caattccttg tcttaacgtt ttttcttaat
     1441 acagtatatg aataggaaga tataattgga attcgaaaaa tttccattgc aatactgaga
     1501 ggactttagg caaaaaaaga cccaggccgt accgcagaaa gtacagcctg ggtcaaaagt
     1561 ttttaaaagt ctattaaaag cagcatgaat taatcatcgg agctaggcaa aaaaggaata
     1621 agccttgctg ccaagttgtt aataggcatc gactgcagcc acacctttcc cgggccttcc
     1681 agggtagtca agaacaagcc ttcccctccg aacagaatat ttttaaaacc ttttaccatt
     1741 tcaatattaa agtctacact gggttcaaat attgccacgt gaccggtatc aacttttaat
     1801 ttttcgccgg gctccaattg atattctact gcttccccgt ctatttcaag gaaagcccaa
     1861 ccgggtcccg ttattttttg tagtatgaaa ccttctcctc caaaaagccc tgcccccaac
     1921 ttcttcttta agtgagcgga tagttttaca ctgcgttcag cacagaggaa agactgcttc
     1981 tgacatataa ttgattcgcc ggcttcgaga tgtattggaa ctatttttcc tggaaaagat
     2041 gacgagaagg ctatgattcc cgatgccttt ttcatggtat atgtagtcat aaaaacactt
     2101 tctcctgcca gggtcctggc aattccacca aagagtctgc cttccatatt tgttttcatt
     2161 tcaaatccgt cactcatcca agacataccg ccggtttctg taaagatgct ttctccctgc
     2221 tctaatgtca agattactac cggaagtgta tctcccctaa gctcgtattt cacaaaaccc
     2281 ccccccttgc tagattataa ttcaataata tcacagggca agggacaaat aaacactttt
     2341 ttttgtgaat ttcgataaat tttttacact tttagattct aacaatgtgt gcatttgaca
     2401 atgcatcata atgaatgtag ccgcctatat ggagtatggt atctcctact tccgggtcga
     2461 ttgtcatgcc gccaagacaa ggataactat gccaaaactt ggctaccgca gcttttgctt
     2521 catctcccag ttttttacct accagatgtt tgcatacatt gtagccgttg ccgcatgggg
     2581 cagaaattat gacttcagct cttttgatag tatcattatt atccacatac agtttaaact
     2641 tcggcttgcc tattttaaac ttgtcaataa actcatttat tatcggaaat tttccgtagc
     2701 taaggttgca aaatggtttt ggaaaggcgt attcaagacc gaacctcttg catctttcta
     2761 tcacttcatc tcttgtccat ctggaaaccc actcaccgct ttcacagggc acgatcaagg
     2821 ctttcccacc gctttctttt attagtttgg gcaattcaac taatatgtct tggtgaatac
     2881 ctatagctat ggcaacatcg tgcttttgaa agctttccgg taagtaatca tcggtattgt
     2941 ccacgaatat ctcgtatatg tcgggcaatt ttatgattcg gactatatcc tctttaaaat
     3001 ctaggtcgta tcttcccctg caccaaaaac agtctttccc acatacggta cacacttcct
     3061 ttttatttag cagatgagga atgaatttcg tattggcaaa tctggtatca gtttttattt
     3121 caaatgagtt cttgggctgc gtatttgatt tttccgagca gattattaat aatttcatga
     3181 taatgccccc cttaaatgcc tcgatttaat tatacatgtt attaaagtta gagtatgtga
     3241 tattcgtcac aatgtattta acagatttat ctgttctgtt gtcaagagag ttttaaagcg
     3301 ttaaggaaac ttgaaaaaga atttgaatat aatgagataa actgatcgct tgaaggagat
     3361 gggaacatga gtattgccgt agctgtttcg gcacaaggct atggagacga aggagctttt
     3421 gactacaggg ctgaaatgac aggccatata attattaaac ccagcacggg gcaagaattt
     3481 ttagacatgt tggcagattt atcgaagagc cgtggcccta tatttttaat gaaagtgttt
     3541 agtcattcgt atactcgagg aattattatg acaaattgga gcggttttta tgacgagccg
     3601 ggacctgaag acacaaaaaa agccgcttat ttaagggact tggctgacag gattcaagaa
     3661 ggaaatatca ggtttgcacc ggattcacag attttattat tcggctgtga cttgggtgct
     3721 ttttctcaaa aactttcagc cgtcaccgga ggtaccgtag ttggatctga tggcggaact
     3781 tatcctgaaa tttggggcaa ccgggaaacg ggcgtgtttc taaccaccga tgactggcta
     3841 gtctataaaa atggcagttt cgcctacagt gcaggtaaac gataccgagc ttggtgagct
     3901 ttacttgttt ttgctggtgc ctagtagcac tgttctcagt ggcgtcttat ccacgatata
     3961 acaaaatata taagaacaga taacggtaag ttttgaatag tatatgtaaa tggaaaatcc
     4021 gggtggtata tgtgccatat cttagaccca cgagcttttt tactaccgtt agtatttgtg
     4081 gatgtacaaa gaatactcca taggagtacc ttgaagcggc tgtaaaaaac tttgcagcta
     4141 tactagactt ctttacgatt tgcgctacga tgaataataa gggaatggcg gccattgaat
     4201 taatcaagac cgtgggtcta gttgaagtat acacgcctaa ataagtttga ccgtatgtta
     4261 aaaatcctag gtaagcctca agtatgacca gtaaagtgga gataatgtat aataatgcta
     4321 tagggaattt gttttgtgtg atttctttaa acatgaagtt ggctgttatt gctttaattg
     4381 gtttttttat cccttttgtg tgtatttcac cgccaaaagt aagccttgct ttatcgtaac
     4441 cctggcagta tttcccaaga aaacattgct aattcctagg gtaggaccca tataaagggt
     4501 ctcccccttc aaagtgtagt ataaaccttc ggtatttaca cctgcgactt cggggctcaa
     4561 cggaataagg gacagaatat cacctttttt cccagttact tctgccatct tgtctattaa
     4621 aaacatctcc cagttttcac ccacaagttt aagcttcagc cctcggttaa agaagttgac
     4681 cattaaaaaa atattggcaa ggccgtggtc gggcctgtct ccgagcccgc ccaagagtat
     4741 ggcttcactg gcaccttttg caagggctat atcaagggct aattcagtat cagtggagtc
     4801 tttttctgca ggaaagttgt gtcttgctat acccaatttg ttggcctttt ctaaagtatc
     4861 gtggttaatg gaatccatat ctcccacaag caaatccggc ataattccca tgttaaaggc
     4921 gtgctctatg ccgctatcgg cacatatgac taaatcggac ccttctatat attttttcag
     4981 tttgtcatag tttttaattt gaccacctgc aaatataaca gctttcatcg tatgcctcca
     5041 taataaatga attagcttct cggctatcga tataattaat tatttcagtc aacacggcaa
     5101 tttggttaaa ataagaaatg tttgctagtg ctgcaatgat ataatccttg tcagcattat
     5161 acatcaataa tcctccatag ccgggaatcg ttcctgtatg accaatcaat acatggcccc
     5221 ctatttcaaa ccttcgcagt cccaggccgt accctgtttg aacatacaga tcttcatcgg
     5281 tacaatctat aaaagtcatc atttcgtcta agtacttgtc ggaaataatc tctcccgata
     5341 ataaagagct ataaaactct atcaaatcat tgcagtttga aacaattgcc cctgctgaat
     5401 aagctaaact tgacaaagaa tcattgaatg tctctattct ttttatccca aggggaatta
     5461 catccctgtc ataaccctta actaattttt ccggtgtctc atcatagggt aatatgcatg
     5521 tattttgtaa attcagctta tcaatgattt tatcatataa taactcttca tattccatcc
     5581 ctgtagcttt ttcacatatt atgcctagta atacataatt cgaatttgaa tacctgtact
     5641 cttctctagg ttcaaaagca ggtttcccct tgagaatata ttcataaatc tccttttctt
     5701 ccacaatttt ctgggattaa gcaaagcctt gcattgcaat gcaaaatttt cagtgtaact
     5761 atataccccg ctggtatgat ttaacaaagt gcgaattgta atagtctctg aattgataag
     5821 ttcgggaaac catttgtcta ttgtatcatc taaattaaaa taaccctctt cagccagctt
     5881 taaaacaagg gtggacgtaa agattttcgt cacactagct attttaaaga cattatcttc
     5941 ccctaccctt tcagttcttt cataatctac agtgccaata ttataattgt gcacatcccc
     6001 atatattttt atagctacct gcataccggg aatttctaat tggtttaatg tatctatcaa
     6061 tatacttgta agttctgcat caaatgtgct gttattttcg tcctgattta tttcataaaa
     6121 tacgctcagc cttggatgtg atttaatatg caaaaacaca aaaataatca ctaataatac
     6181 aacaatagtt attgattttt taaatcgact gatcacttta tttcactccg cttttgtttt
     6241 gccaaaaccc ttgccgtttc aaaacataaa tatttagtcc ctctgtattc aatacggtat
     6301 taaatccata attcctgcaa attcaacaat tatatttgat gttgaaaatg tgaaagcttt
     6361 tttgagtttg gatttttccc ttgcaaatgg tagaatcaag ataaagtgtt ttaacaagga
     6421 gatgcctatg aaaactataa atgttctgta tgaagataat catttgctgg tagtaacaaa
     6481 accgcctaat ctgctatccc aaggtgacaa aacaggagat cctgatttgc ttagcatttt
     6541 gaaagatgac ttaaaagaac gccataataa gcccggcaat gtatatctcg gcctggttca
     6601 ccgcttggac aggccggtag gtggagtcat ggttttcgcc aaaacatcaa aggcagctgc
     6661 caggctttca gaccaggtaa ggagaaatct gtttaaaaaa acttatatag ctgtgattcg
     6721 gggcttgcct ccaagggaaa aggatacact gattcactat ttattaaaaa atacaaagac
     6781 caacagggtt gaagctgtga aaaaaggagt taagggagca aaagaggcca ttctggatta
     6841 cgaagtaata agttatgccc acccttattg cctggtaaaa attgatcttc ataccggaag
     6901 gccgcaccag atacgagtcc agttttcaaa aatcggttgc cctatcatag gcgaccaacg
     6961 ttatggatat aaagacaaat acaaccatat agccctgtgg gcttataaaa tttcctttga
     7021 acatccgaca ttgagaaaaa aagtagagtt ttctgccttg ccggaaaatc ggccgccctg
     7081 gtcccgtttt tcactggcca aattaaaata actatcttta tatgacaatg tattaacttg
     7141 aaatcttgaa acagataaaa gttttcattt tcctcattga ataccaaaat attatctgat
     7201 ataatgggga tgatttaaat agaaagaatt gaagggagtt gtgttttttg aaagaaaagg
     7261 ttcaagctgc tttagataaa attcgcccgg ccttgcaagc cgacggtggc gatgtcgaat
     7321 tagtagatgt tgatgaagcc agcgggatcg ttaaagtaag gcttaccggt gcctgcggcg
     7381 gatgcccgtt tgcaacaatg actctaaaaa atggcataga agaaacatta aagcaagtaa
     7441 tacccgaagt tcaagaagtt cagcaagtgt tctagtttac actatggggg ccagcaaagc
     7501 tggccccttg ttgcatgtcg tattattata gtcaaaagca agcaactaca accagcccat
     7561 acaatgttgt aacataaata gtggtagggg cgacctgtgg tcgcccgcta agtaaaacct
     7621 tataagtgat aagtattgtt cacagtgact ataaaggcat cgctttctgg cagttctttt
     7681 tgaccatcat attcgatgat ggtaactgat gtgttgctaa ttttaaactt ccagaaaaaa
     7741 tcctgtctta gattcaatag caccgatagt atcgccctga tggggccagc atgacttacc
     7801 acaagtatcc ggccgtcagg atgcttattt gctgcttctt tcaaaaactc tgaaactctc
     7861 tcgcaaaggt ccgataggct ttccccctct ggaggagttt tattgtaagg atcagcttgc
     7921 cagtctctat attcttgagc ccatttttgc atgatctcat cataggtcat accttcccaa
     7981 agtccgaagt tcaattcccg catcaaggga gccctcaaaa ctttcagtcc atgcggttca
     8041 gctatgattt cggctgtttc tactgttcgt tcaaggtcgc tggcataaat cgcatcgatt
     8101 ctctcatgct taagtctttc ggaaagaatc ctggcctggg cccttccttc atctgttaaa
     8161 ggtatgtctg actggccctg gtatttgagc tgtctattcc acatggtttc accatgtc
//
LOCUS       DAONUO010000048         8126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6730_length_8126_cov_3.212613, whole genome shotgun sequence.
ACCESSION   DAONUO010000048 DAONUO010000000
VERSION     DAONUO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6730_length_8126_cov_3.212613"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2919
                     /locus_tag="PK768_02795"
     CDS             <1..2919
                     /locus_tag="PK768_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HQA59931.1"
                     /translation="GYGVNSWLITTALKLNKKICEVELGAKLSPVSLGKKYIVYKEML
                     KALFECIKRDEDFWLRDSETIRAPDIFTRGQEDEALEVCCNYKEHLDSFLEGTYHYEN
                     LLQKILSTEEKRCIEDMTRAYATPGYSPDFWAKIAYKFLLTDNFCPEISQEDLLELFL
                     AIYNGMVALLIMEDEKIKKSLKAADIDIDKIALSQLEGIYNDTIRAFAKNKGHFVKAW
                     RERVDATSPALTLLDYLEFIPGVPIVLPKKLYGRGKREVITNQVFKRLQRKYNSAFMS
                     FLDSINLDEKASSQEIGLCLTRVMEELERTVDSLCPGHLYSVKGTREVVNHIFEIFPH
                     NKVFAVKWQVLRKLLHEYPPGNLILRMGFRSLPELLDNMDEREILTLATFTEDKDYFD
                     RIFYWLKDNLRADSFEEVEILPMIVDRTNFHGFRELREISDLNRLTARIPVMNLGKGM
                     GGKYPKLRYFTRISKSIVEAEHFSGLWKNYARERKEVGRKFVNSILGHYGKAMFSAHH
                     IFENWHHRVLVSRLKSVSDKLRELGRERDAQNIDTMIEGYGLSLVLNDGTFMPCSAWT
                     WASFSFKGGEGIPTALFLHVERDWFNHDLLEEIYKEMGYNPDEILNQVFLYISRGCES
                     IDLAQVLLGVKPYREEVVIQELDYWPKAKELKRYSETPILLPIQEHYWESRFVLNTAA
                     LRIKDKVYLLYRAFGDDGISRIGLAISDGYRIIERLTEPVFGPEIKEEKKGCEDPRTV
                     IIDDEIFMLYTAYDGVVAQIASASISVEDFLNRKFDRWKRRGLAFPGLWDKDAILFPE
                     KIDGKYVIYHRIEPSIWIAYSDELTFPWPKNGHKIIMGPRSGMMWDSLKIGAGAQPIK
                     TRYGWLLIYHGVDQDMVYRLGVMLADLKDPGRLLYRSPNPTLSPETEFEIGKKGEYWV
                     PNVVFTCGAVPAEDKDILDIDDEILVYYGASDTYICLATGTVGDMIPQQVRERILSRR
                     "
     gene            3325..4011
                     /gene="ftsE"
                     /locus_tag="PK768_02800"
     CDS             3325..4011
                     /gene="ftsE"
                     /locus_tag="PK768_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HQA59932.1"
                     /translation="MYGVTKVYPGGQIALKDINLKICKGEFVFIVGPSGAGKSTLIKL
                     IFREEVPTKGQIFFAGKNITRLMHREVPHLRRRIGIVFQDFRLLPEKTVYENVAFAME
                     VVEAHTREIRKRVPLVLERVGLLHKAKAKPSELSGGEQQRVALARALVNNPDILVADE
                     PTGNLDPETAWDIMGLLDDINKRGTTIIVASHAKELVDSMKKRVIQLENGSLVRDEER
                     GVYNCESKDF"
     gene            3992..4654
                     /gene="ftsX"
                     /locus_tag="PK768_02805"
     CDS             3992..4654
                     /gene="ftsX"
                     /locus_tag="PK768_02805"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HQA59933.1"
                     /translation="MRVRTFKYFFKESFTSLIRNRWMSLASIGAVASALIVFGSFLLL
                     SVNFDYILKDVESQVEITAYLEDSVEQESIAKLNRDIAAISEVKEIKFISKEEAIEEF
                     KEQVGKELLEGIENPLPNSFRIKVNDPHEVAAVAEKIERFAGIEEVKYGKGIVEKLFN
                     IIYWLRMIGLAIMIVFACVSIFIISNTIRLTVFARRREISIMKYIGATDWFVRWPFLI
                     EG"
     gene            4651..4863
                     /locus_tag="PK768_02810"
     CDS             4651..4863
                     /locus_tag="PK768_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59934.1"
                     /translation="MILGLIGSLVAIGVLALAYKYLYITVKLNIPMISLLPIEGFYEY
                     ALGFLGIGMLIGAFGSSFSIKRFLNV"
     gene            4884..6023
                     /locus_tag="PK768_02815"
     CDS             4884..6023
                     /locus_tag="PK768_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQA59935.1"
                     /translation="MNRKRYRLVAGFLLLVLLTISVPAIASDIDELRKQQNMLNSQLE
                     NLKNSIKQVDKEKKNVAEEIAELEQKLSEAQKELAATEAKLKETQAKLESTMKELKQA
                     EERVEEQKDDLNTRMRTMYKTGPVDYIEVILGASSFSDFLTRLDMVERIIEADKSLLA
                     EFRARKEEVAQKKSELEEQQRLIEAQRNSINERRAQIVAYRGDRQRLMAKLEEQKREY
                     ERQQDQLERDAENLRRKILAWEMQNKKGFFGTGEFRWPVPSSSYVTSDFGWRIHPIYK
                     TRRFHDGIDIAAAMGADVLAADDGEVIFAGSYGGYGNTIIVSHGGGISTQYSHLSKIL
                     VGVGKQVIKGDKIGLVGSTGLSTGPHLHFSVIKDGQAVSPWNWLK"
     gene            6070..7302
                     /locus_tag="PK768_02820"
     CDS             6070..7302
                     /locus_tag="PK768_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQA59936.1"
                     /translation="MSNYKARPLLKVAILCVAVSLLSFFAGANFGKSSPKISEEDINN
                     LVAAAISEQHEMEPLKPILDVLKIIENKYFRDVDTEKLIEGAIRGMVASLEDPYSVFM
                     NESEFRDFITALNGAFGGVGIVVGADENTGEIIVLAPIEGTPAYKAGILPRDVIVKVN
                     DVDLAGKSLDDAVRLLRGEPGTQVTVYVKRADEHEPLKYQLTRDDIRLSTVSHEVFED
                     DIGYIRISTFDARTYSEFKSTLEDLENKGIKALILDLRNNPGGSIYDSVLIADELLGE
                     GLIVYTEDRHGNRLEEFISDEDKISVPLVVLINENSASAAEIVAGAVQDHKAGLLVGT
                     KTFGKGSVQEVHPFENKSGMKLTIASYYMPSGRCIDGIGVEPDVEVELDEGTNLYNVP
                     RDKDYQLIKALEILKSHP"
     gene            7326..>8126
                     /locus_tag="PK768_02825"
     CDS             7326..>8126
                     /locus_tag="PK768_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQA59937.1"
                     /translation="MFPFGRILFLILQGLPSSFFNPFFWLVIFIVWSQYRRTAEIERN
                     LFGREKISPRDKILYAVLYGICGGVLGSLLVMVLGISITEAGLMFVWPLALLLAIIDP
                     HLMCFSYAGGIVSLFSLLTGLIRIDVAGLMGLVAVLHFVESILIYFAGHINATPVYVK
                     DKRYGVIGGFSLQEFWPVPIMLLTIITGDFPPIDVVQMPDWWPIIKPPAHILQGDQAI
                     YLMLPVVAALGYGEVALSRTPKNRCRSTSLNLLLFSTSLLLLAVLASRY"
BASE COUNT         2361 a         1627 c         2051 g         2087 t
ORIGIN      
        1 ggttatggcg tcaattcctg gcttattact actgcgctaa aactgaacaa aaaaatatgt
       61 gaggttgagc tcggtgcaaa actatcaccg gtttcgctag gcaaaaaata tattgtctac
      121 aaggaaatgc tgaaggctct gtttgaatgc attaagagag atgaggattt ctggctcagg
      181 gactctgaga ctataagagc tccggatatt tttacaaggg gacaggagga tgaagccctt
      241 gaagtttgct gcaattacaa ggaacatctg gattcgtttt tagaggggac ttaccactac
      301 gaaaatcttt tacaaaagat attaagtaca gaggagaaac gatgtattga agatatgacg
      361 agggcctacg ctacgccagg atactcaccg gatttctggg caaaaatagc ttacaaattt
      421 ttgctgaccg acaatttttg ccctgaaatc tcgcaggagg atctgctgga gctctttctg
      481 gccatatata acggcatggt tgcactgctg ataatggaag atgaaaagat aaaaaagagc
      541 ctcaaagctg ccgacattga tatagataaa atagcattat ctcaacttga aggaatctat
      601 aacgatacta tcagggcgtt tgcgaaaaat aaaggacact ttgtaaaagc ttggcgcgaa
      661 agggtagatg ccacaagccc ggctctaaca ctcctggatt acctggaatt tatacccggc
      721 gttcccattg ttttgcccaa aaagctctac ggcaggggta agcgggaagt cattaccaac
      781 caggttttta agaggctcca aagaaaatat aactctgcct ttatgtcctt tcttgacagc
      841 attaatcttg atgaaaaggc ctcatcccag gaaatcggcc tatgcctgac ccgggtcatg
      901 gaagagctgg agcgtaccgt agacagcctt tgtcccgggc atttgtacag cgttaagggc
      961 acccgtgaag tggtaaatca catttttgaa atctttcccc acaacaaggt ttttgccgtg
     1021 aaatggcagg ttctcaggaa gctcttacat gaatatcctc cgggaaattt gattctgagg
     1081 atgggcttta ggagcttgcc tgagctcctt gacaatatgg atgaacgaga gatacttaca
     1141 ttggcaactt tcaccgaaga caaggattac tttgacagga ttttctactg gctaaaggac
     1201 aacctaaggg ctgacagctt tgaagaagtt gaaattttgc ctatgatagt tgacaggaca
     1261 aattttcatg gcttcaggga acttcgggag atttcggatt taaacaggtt aactgcccgc
     1321 atccccgtca tgaacttggg caaggggatg ggtggcaagt accccaagct cagatatttt
     1381 acccgtattt ccaaaagtat agttgaagcg gaacattttt cgggcctttg gaagaattac
     1441 gccagggaac ggaaggaagt aggccgcaaa tttgtcaatt ccattttagg ccattacggt
     1501 aaggccatgt tctcagcaca tcacatcttt gaaaattggc accatcgggt gttagtatca
     1561 aggctcaaat ccgtttctga caagcttcgc gagctgggca gggaaaggga tgcacaaaat
     1621 atcgatacca tgatagaggg ctacggcctc agcttagttt taaatgatgg cacctttatg
     1681 ccttgttccg cctggacttg ggccagtttt agctttaaag ggggagaggg aataccgacg
     1741 gctctcttcc tccatgtgga gcgggattgg tttaaccatg atttgcttga agaaatctat
     1801 aaggaaatgg gatacaatcc tgatgaaatt ctaaaccagg tatttctgta tatcagccgt
     1861 ggctgtgaaa gtatagattt ggcccaggtt ttattgggag taaaaccata tcgggaggaa
     1921 gtggtaattc aagagctgga ttactggccg aaagccaaag agctcaaacg ctacagtgag
     1981 actccgatac ttttgcccat acaggagcac tactgggaaa gcaggtttgt tttaaacacg
     2041 gcggcactta gaataaaaga taaggtatat ctcctatata gagcctttgg cgatgatgga
     2101 atttcacgga ttgggctcgc gatatccgat ggctacagga ttatagaaag actaacagaa
     2161 cccgtgtttg ggccagaaat caaggaagaa aaaaagggct gcgaagaccc ccggacagtt
     2221 ataatagatg atgagatttt tatgctctac actgcttacg acggagtggt agcgcagatt
     2281 gcctcggcat ccatttcggt agaggacttc ctaaacagga agtttgatcg ttggaaaagg
     2341 cggggccttg cctttccggg cttatgggat aaggatgcca tactgtttcc cgaaaagatt
     2401 gacggcaaat atgtcatata ccaccgcata gaacccagca tatggatagc atattcggac
     2461 gaattgacat ttccatggcc caaaaatggc cacaaaataa tcatgggccc ccgttcgggg
     2521 atgatgtggg actccctgaa aataggggcc ggtgcacagc ccataaaaac ccgctacggc
     2581 tggctgctga tataccacgg agtagaccag gatatggtat accgcctggg cgtgatgctg
     2641 gcagatttaa aggatcccgg acgcctgctg taccgctcgc ccaatcctac attatctccc
     2701 gagacggagt ttgagatagg caaaaaaggc gaatactggg tgcctaatgt ggtctttacc
     2761 tgcggtgccg ttcccgcaga agacaaggat atcctcgaca tcgatgacga aatcttggta
     2821 tactacggtg cctccgatac ctatatctgc cttgccaccg gcaccgtagg cgacatgatc
     2881 ccccaacaag ttcgtgagag aatcctaagc cgccgctaat attcaaaaca atcgacaagc
     2941 ctgcacatta gattgaagct aattcaatct ttgatgacct gccaagggga cgtttcgttt
     3001 gccaggttgg catcgccaac ctggcaaacg aaacgtcccc ttggcaggtc atcctgtata
     3061 ttagctgaag atttgccgtt atgtggcaag gctattattt tttgcgaaaa agtataatat
     3121 tgtccgcgta taacttttta tgttttttaa gctttgctag aaatgatatt caaatgttga
     3181 gaataagata tatttgatga tatatttaaa atgccgacga agtatgagaa attgctgatt
     3241 taaaactaat ttcttgaagg attttcagta tagacgtcga agtaaaaaat atacctggcg
     3301 tttggagtga ttgtgttgat tgaaatgtat ggggttacga aagtgtatcc gggcggtcag
     3361 atcgccctta aagatatcaa tttgaaaata tgtaaaggcg agtttgtctt tattgtaggc
     3421 cctagcggtg caggtaagtc tacactgatt aagctcatat ttcgagaaga agtgcctaca
     3481 aaaggacaaa tttttttcgc cggaaaaaac attacccgac taatgcatag agaagttccg
     3541 catcttaggc gccgtatagg catagtattt caggatttta ggctcttgcc ggagaagacc
     3601 gtatacgaaa atgtagcctt tgcaatggaa gttgtcgagg cgcatacgag agagattaga
     3661 aaaagagttc cccttgtcct ggagagagtt ggtttactgc ataaagccaa ggctaaacct
     3721 tcagagcttt ctgggggtga acaacagagg gtcgcattgg ccagggcctt ggtcaacaac
     3781 cctgacatcc tggtggccga tgaacccacc ggcaatctgg atccggaaac tgcatgggac
     3841 ataatgggcc tcttggatga tattaataaa cggggaacca caataattgt tgcttcccat
     3901 gctaaggaac tggtggattc catgaaaaaa agagtaatac agttggaaaa cggcagcttg
     3961 gtcagggacg aggagagagg ggtatataac tgtgagagta aggactttta aatatttttt
     4021 caaggaatcc tttaccagtc tcattagaaa cagatggatg agccttgcat ccatcggagc
     4081 tgtagcatct gcccttatcg tttttgggtc tttcctactt ctttccgtaa attttgacta
     4141 catattaaaa gatgtggagt cacaggtgga gataacggca tacctggaag attcggtaga
     4201 gcaggagagt atagccaagc taaataggga tattgcagca ataagcgagg ttaaggagat
     4261 aaagtttatt tcaaaagaag aggctatcga agaatttaaa gagcaagtgg gtaaagagct
     4321 gttggaaggc attgaaaacc cccttcccaa ctcatttagg ataaaggtta acgaccccca
     4381 tgaagtggca gcagttgctg aaaaaattga gagatttgcc ggaattgaag aagtaaaata
     4441 cggcaagggt atcgtcgaaa aactctttaa catcatttat tggctcagga tgatcggctt
     4501 agcaataatg atagtctttg cctgtgtttc aatattcatc atttcaaata cgatacgact
     4561 cacggtattt gcccgtagac gcgagatcag tatcatgaag tacatcggtg ccacggattg
     4621 gttcgtgcgc tggcccttct taatagaggg atgatattgg gcttgatcgg ctccttggtg
     4681 gctataggcg tattagccct tgcttataaa tacctgtaca ttacggtcaa gctcaacatt
     4741 ccaatgattt ctctgctgcc tattgaagga ttttatgagt atgccctagg ctttttgggc
     4801 atcggcatgt tgattggagc ttttggcagt agcttttcaa taaagcggtt cttaaatgtc
     4861 taaagccttg gaggaggaga aacatgaacc ggaaaagata tagattagtc gccggctttt
     4921 tgctgttggt attgttgaca atctccgtgc ctgctattgc cagtgatatc gatgagctgc
     4981 gcaagcagca aaacatgctc aactctcaac tggaaaacct gaaaaacagc ataaaacagg
     5041 tggacaagga aaagaaaaat gttgctgaag agatcgcaga gttggaacag aaattgagcg
     5101 aagcacaaaa agaactggct gccaccgaag ccaaactcaa ggaaacccaa gcgaagcttg
     5161 aaagcaccat gaaagaactg aagcaggcag aagaacgggt ggaagaacag aaggacgatt
     5221 taaacacacg aatgaggacc atgtacaaga caggacccgt tgactatatt gaagtaatat
     5281 tgggtgcttc cagtttttcc gattttttga caagacttga catggttgag cggattatag
     5341 aagctgacaa gagcttgctc gccgaattca gggcaagaaa ggaagaggtg gcccagaaaa
     5401 agtccgagct tgaagaacag caaagattaa tagaagccca acgtaacagc atcaatgaga
     5461 gacgggcaca aatagtcgcc tatcgcggag atcgtcagag gttgatggcc aagcttgaag
     5521 aacaaaagag ggagtacgag cgtcaacagg accaacttga gagggatgca gaaaaccttc
     5581 gccgtaaaat actggcctgg gaaatgcaaa acaaaaaggg ctttttcgga acgggagaat
     5641 tccgctggcc agtgcccagc tcctcatatg ttacttcgga ctttggatgg agaatccacc
     5701 cgatttataa aacgcggaga ttccatgacg ggatagatat cgcagcagca atgggtgctg
     5761 atgtgctggc ggcagacgac ggcgaagtaa tctttgccgg ctcatacggc ggctacggca
     5821 acaccatcat cgtcagccac ggcggcggta tttcaaccca atactcccat ctttccaaaa
     5881 tactggtagg cgtgggtaag caggtgataa aaggcgacaa aataggcttg gtgggcagca
     5941 ccggattgtc cacaggaccc caccttcatt tttcagtaat taaagatggc caagctgtta
     6001 gcccttggaa ttggttaaaa tagatgttat aatggtaaag actaaatact ttaccgggtg
     6061 ggaatacata tgagcaatta caaggccaga cctttactaa aagtagcgat attgtgtgtt
     6121 gcagtatcgc tgctttcgtt ttttgccgga gccaattttg gaaaatcttc tcctaagatc
     6181 tctgaagaag atatcaataa tctggttgct gctgcaatca gtgagcaaca cgagatggag
     6241 cctttaaagc caattttaga tgtgttaaaa attatagaga acaaatactt tagagatgtt
     6301 gacacagaaa agctcataga gggggccatc aggggaatgg tagcttccct cgaggatccc
     6361 tattctgttt ttatgaacga atcggaattt cgagatttta ttacagcttt aaacggcgcc
     6421 ttcggtggcg tagggatagt tgtcggagct gacgaaaaca cgggtgaaat catcgtactt
     6481 gctcccattg agggaactcc tgcttacaag gcaggcatcc ttcccaggga tgttatagtt
     6541 aaggtaaatg atgtggattt ggccggaaaa agcctggatg atgccgtaag gcttttaaga
     6601 ggggaaccgg gtacccaggt gacggtatat gtaaaaagag ccgatgagca tgagcctttg
     6661 aaatatcaat tgactcggga tgacatcagg ctgagcaccg tcagccatga agtttttgaa
     6721 gatgatatag gatatataag aattagcact tttgatgcca ggacctacag cgagttcaag
     6781 agcacccttg aggacctgga gaataaagga ataaaggctt tgattttaga cctcaggaac
     6841 aatccgggcg gttcgattta cgattcggtg cttatagcag atgaattatt gggagaaggc
     6901 ctaattgtct acacagagga taggcacgga aaccgattgg aggagtttat ttccgatgag
     6961 gataagattt cagtgcccct tgtcgtgctg ataaacgaaa acagtgccag tgctgccgag
     7021 atcgtagcag gtgcagtaca agatcacaag gcagggctgt tggtaggtac aaaaaccttc
     7081 ggcaaagggt ccgtacagga agtccatccc ttcgagaata agagcggaat gaaattgaca
     7141 attgccagct attacatgcc cagcggccgc tgcattgacg gcataggggt agaacctgac
     7201 gtggaagtag agctggatga aggcactaac ctatataatg taccaaggga caaagattat
     7261 cagctaataa aagcgctaga aatcctcaaa tctcacccct gatggggttt acgggagcga
     7321 tgaaggtgtt tccctttgga agaatactgt ttctgatatt acagggctta cccagttctt
     7381 ttttcaatcc ctttttttgg cttgttattt tcatcgtgtg gagccaatac cggagaactg
     7441 ctgaaataga gaggaatttg tttggaaggg aaaaaataag tccgagggac aaaatccttt
     7501 atgccgtgct gtacggcata tgcggcggcg tcctgggcag cttgctggtc atggtcttgg
     7561 gtataagcat aacggaagct ggccttatgt tcgtgtggcc tttggcattg ctcctggcca
     7621 ttattgatcc gcacctgatg tgtttttcct acgccggagg tatcgtctcg ctgttttccc
     7681 tgctgacggg tttaataagg attgacgtgg cagggctcat gggcctcgta gcggtgcttc
     7741 attttgtgga aagcattttg atatattttg caggccacat aaatgcaacc cctgtttatg
     7801 tcaaggacaa aaggtacggc gtcattggag gcttttctct acaggagttt tggccggtcc
     7861 ccattatgct gctgaccata attacggggg attttccacc gatagatgta gttcaaatgc
     7921 ctgactggtg gccgattata aagcctcccg cccacatatt acagggcgat caggccatat
     7981 acttgatgct gcccgttgtg gctgcactgg gctatggtga agtagccctg agccggactc
     8041 cgaaaaatcg ctgcaggtcc acttcgttaa acctcctgct gtttagcaca tcactgcttt
     8101 tacttgccgt actggcctcg cggtac
//
LOCUS       DAONUO010000049         8110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6749_length_8110_cov_3.673495, whole genome shotgun sequence.
ACCESSION   DAONUO010000049 DAONUO010000000
VERSION     DAONUO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6749_length_8110_cov_3.673495"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK768_02830"
     CDS             <1..234
                     /locus_tag="PK768_02830"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59938.1"
                     /translation="GLFDICLLGDISRLEIIKFLPRVFSGSHKEHKAFEVMRGKEVKI
                     EFETPRLVQTDGEIIGYTPARFSVLPKALKVIR"
     gene            289..582
                     /locus_tag="PK768_02835"
     CDS             289..582
                     /locus_tag="PK768_02835"
                     /inference="COORDINATES: protein motif:HMM:NF015643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HQA59939.1"
                     /translation="MSLLVALEEGLEEIKEYLRSKGLQTVNLGNENMAVDAVVYKDRS
                     LSEIQAEQILSSADSLQDYAGGNSYGVLLVNARDKTPQEVYEIIKNRVYERFI"
     gene            complement(810..1385)
                     /gene="yyaC"
                     /locus_tag="PK768_02840"
     CDS             complement(810..1385)
                     /gene="yyaC"
                     /locus_tag="PK768_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HQA59940.1"
                     /translation="MFVKQTCVKAREFRIHIDSPSAYKLFRSFFVKTLADTYNAGPLV
                     IMCIGTDRSTGDALGPLVGERLHKTCRHARVFGNLKEPVHAVNLEKILDNIRDTYENP
                     FIIAVDASLGPSENVGTIKIAPGSLKPGAGVNKKLPPVGNFHITGVVNVGGFMEYLVL
                     QNTRLFTVMKMVDIISLGMRDGLDIFFGKGQ"
     gene            1512..2552
                     /locus_tag="PK768_02845"
     CDS             1512..2552
                     /locus_tag="PK768_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59941.1"
                     /translation="MNLRTEIGLGAIYMGTVIGAGFATGQEIMSFFTVYGKQGLLGIA
                     LASALFFLLGYLVLLQSIRKQSQCLRDILLPLAGERLTLAFELMADVFCLAGYCIMLS
                     GCGAVLEESWNLNYTMTIFLLSVFLIWCLSRELSGLAAANKLLLSVILALTIVIGSAC
                     ISDRQGISETSLQGEKGSWLMSSLLYVSYNGTLALVVLSSLGSYTDRVGAALGGAAIG
                     ALGIMLMAGLMWFITWVNYPGLYGAQVPLLQVARPLGNLLFLSSVIVLLAAMITTALG
                     LGFAFARSVSKRFGLSYKRAIYFLLIGIPLAKYNFAALINTIYPFFGKLGIIFAIFLA
                     IKQVFKHENRMI"
     gene            2589..3677
                     /locus_tag="PK768_02850"
     CDS             2589..3677
                     /locus_tag="PK768_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59942.1"
                     /translation="MKERSALRPSIYCGLLLLLVLAITLLVNNKPIGYSKNALFQTLQ
                     PKILLEVQGDLAWAGGDSYYNYSDGSLKMMEETGKVLWDTKIKGQLLWIGEEGVIAAQ
                     DNMLLMLDGNGHEIFQKADLPDKPRVLCVQGKYLLLSGKLSGVEHGILLHDRGDIVWQ
                     IPLDGSIISGSLHPKGIYAVFSVIDNDAKSRLVVVGPRGDILIDSGHQLPLYRIRAVS
                     QGICVITADRAYLMDYDGRLLWEYGFQGQVLRGDIGSDGSVAAIVEEHAGSLSQDLHQ
                     VLIMLSEQGRHICSYSLDSRPNNVKVDKDRVYIVDDYGIMLLSREGLLLSKITQRGIK
                     KLTFTDKNNIIVNLEASSLLLESPTGGI"
     gene            3679..4245
                     /locus_tag="PK768_02855"
     CDS             3679..4245
                     /locus_tag="PK768_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015296008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQA59943.1"
                     /translation="MNWLDIVLLIIIIKSALQGYFKGFVLAAFKTAGVIVSLFMGIFY
                     RDEAVSFLKAQLAMDRHISSMMLSPAMRDLNTLGVANIRNIIDLMLAAVGFLLIFLLA
                     QVIFLVPAYLISGIVKLSNLSLFDRLLGGLFGIARAALWLALLSAVTSPFLLLFPGSI
                     LDRGISSSYILNHLRFLDFISPIVVKLI"
     gene            4262..5119
                     /locus_tag="PK768_02860"
     CDS             4262..5119
                     /locus_tag="PK768_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQA59944.1"
                     /translation="MGELVQTYNNLINQLPTSAKSLVRIAVVLLVSALIIKLANEFIT
                     RFLFMPNRDRIKIDENKKKTLASLLKSIVRYVTYFMAAMNILEILGINTTSLLTAAGV
                     GGLAVGFGAQNLVKDIISGFFLIFEDQYNVGDYVELAGVSGIVDEIGLRTTKVRDFGG
                     QFHIIPNGEIKLVTNHSRGMKRALVNINIAYEEDLNKVLKALEEVCQQVKEKREDILE
                     GPSVLGISNLGPSEVVVTILARTEPMQQWSVEREIRKAVLEKFGKEGIEIPYQRIVYI
                     NKQSKGEVQ"
     gene            5116..5322
                     /locus_tag="PK768_02865"
     CDS             5116..5322
                     /locus_tag="PK768_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HQA59945.1"
                     /translation="MNYELGDIVQMKKKHPCGSDKWQIWRLGMDIGIKCMECGRKVMI
                     PRRKFEKSVKKILTSEQSPPSNNL"
     gene            5627..5914
                     /gene="rpsF"
                     /locus_tag="PK768_02870"
     CDS             5627..5914
                     /gene="rpsF"
                     /locus_tag="PK768_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQA59946.1"
                     /translation="MRKYETIFIVDPNFEADQVNELVEKFKGLIQEQGGQIDGVDDWG
                     KRRLAYPINKQREGYYFLINFTAKPETAQDLERVYKITNGVLRYLIVKNEK"
     gene            5927..6349
                     /gene="ssb"
                     /locus_tag="PK768_02875"
     CDS             5927..6349
                     /gene="ssb"
                     /locus_tag="PK768_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQA59947.1"
                     /translation="MLNRIVLIGRLTRDPELRFTPANGVPVTQFTIAVDRPYVNQKGE
                     READFIRIVTWRKLAEVCANNLTKGRLVGVDGRLQIRSYEGQDGQRRYITEVVADSVQ
                     SLDRPKSGSEDSSFDSMDADFSFDPGIDDQIEGDGVPF"
     gene            6371..6595
                     /gene="rpsR"
                     /locus_tag="PK768_02880"
     CDS             6371..6595
                     /gene="rpsR"
                     /locus_tag="PK768_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQA59948.1"
                     /translation="MKPKRVKKKKVCSFCVDKIEFVDYKDHGRLRKYITERGKILPRR
                     ITGNCAKHQRQLTTAIKRARNIALLPFTAD"
     gene            6641..7585
                     /locus_tag="PK768_02885"
     CDS             6641..7585
                     /locus_tag="PK768_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HQA59949.1"
                     /translation="MKPTNTKALVEGALLAAINIVLSIMALYMPILGSFATLVWPVPI
                     VILGIRHGLRTSILATAVAGIIVAILSGPFQALSIVISFGLIGIAMGGAINKKLSPFK
                     VLAVGGAASLISKVILIFLTMLMMGINPITEEINIMKESLLMAGSLYEKFGMDPQTVE
                     TTIDSFNRSLELLPILIPGIFIMASVMDTFLTYVVTKAVLARMGQKLEDLTPFWLWKF
                     PDFTVAFFLLGYLLVLLETYWPVGVLKAVGMNISLVFGFAFYIEGFSLLAYFLGKYNV
                     AKPLRVLITFFALFNPLFLQLMFFAGLFDILFNFRKIY"
     gene            7601..>8110
                     /locus_tag="PK768_02890"
     CDS             7601..>8110
                     /locus_tag="PK768_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59950.1"
                     /translation="MDRGNDRIILLGGLLYLLGVILICTILIDVKVALLLLPLLGLLL
                     YYLVFVKKEIQSLPALIEPTTSFRQGLWKNTLNELGTAVAIVDKNNKVIWKNKVFIDN
                     FQAADDRIKDIGDIIPPDILNSLNDQCQEIEINERHFSLRSAPLPGSRRGNRGMYRAI
                     FIQDIEDLHE"
BASE COUNT         2263 a         1610 c         2062 g         2175 t
ORIGIN      
        1 ggattgtttg acatttgctt gctgggggat ataagccgcc tagagattat aaaatttttg
       61 cctagggttt ttagtggatc acataaagaa cacaaggcct ttgaagtcat gagggggaaa
      121 gaggtaaaaa tagaatttga aacaccgaga ctggtgcaga cagacggaga aattatcggg
      181 tatactccgg ccagattttc cgtgctgccg aaggccttga aggtaataag gtgattagga
      241 ataattttgc ctccgcctga taacaatata aaggcggagg cgtttattat gagcttgctt
      301 gttgccttgg aggaaggcct ggaagagatt aaagagtact tgcgaagtaa gggcttacaa
      361 acggtcaact tgggaaatga aaacatggca gttgatgctg tggtatataa agacaggagc
      421 ctttcggaaa tccaggcgga gcagatacta tcatctgccg attcattgca ggattacgct
      481 ggcggtaatt cttacggagt gctgctggta aatgcacgcg ataaaacgcc ccaggaagta
      541 tatgaaataa taaaaaatag ggtgtatgag cgtttcatat agagataagc aaataacata
      601 aaacagcggc ccttaagata gtaggacatg gtcgggaaaa aattaagcaa caataccaat
      661 tagaacaatt taataataat taaagagctt gtatatgaaa ataaactgcc ccttcaaaga
      721 ctgtgaaatc ttcccctttg tgtaggtacc ctgacgatga tacaggacta catgaagggg
      781 agttttttta tgagggaaac aaggcttgct cattggcctt tgccgaagaa aatgtccaat
      841 ccatccctca tgcccaggga aatgatgtca accattttca taacggtaaa aagcctggtg
      901 ttttggagca ccaggtactc catgaatccc cccacattca ccacgccggt aatgtgaaag
      961 ttgcctaccg gcggcaactt cttgttgaca cccgctccgg gtttaagaga cccaggggcg
     1021 attttgatgg tgcctacatt ttccgatgga cccagggagg catcgacggc aattataaaa
     1081 gggttttcat atgtatcccg tatattatcc aatatttttt ccaggtttac cgcgtgaacc
     1141 ggttctttaa gatttccaaa tacccgggca tgcctgcatg ttttgtggag ccgctcacct
     1201 accaggggac ccagggcatc tcctgtggag cggtccgtgc ctatacacat gatgacgagg
     1261 ggcccggcat tatatgtatc ggcaagggtt tttacaaaaa agctgcgaaa cagtttatag
     1321 gcagatgggg aatcaatgtg tatgcgaaat tccctggcct ttacacaggt ctgctttacg
     1381 aacatggttt tcctcccgag aaactttgta acattatatg taatacccca ttccagtatt
     1441 catttaaatt atgcgcatag tttctttttt taatcatatt tataggtaaa taaatatggg
     1501 aggggaacag cttgaatctc aggaccgaaa tcgggctagg cgcaatatac atggggaccg
     1561 ttatcggggc aggctttgct accggccagg agataatgag ctttttcacg gtctatggga
     1621 aacagggttt gctggggata gcccttgcct ccgccctctt cttcctgctg gggtacctag
     1681 tactgctaca gtctattcgc aaacaatccc aatgcctaag ggatatactg ctgcccctgg
     1741 caggggagag attgaccttg gcctttgagc tgatggcgga tgttttctgc ctcgcgggct
     1801 attgtataat gctttcgggc tgcggggccg tattggaaga aagttggaat ctcaattaca
     1861 cgatgacgat atttctccta agcgtgttcc tgatttggtg tttgagcaga gaactcagcg
     1921 gccttgcagc ggccaacaaa ctgctgctat ccgtgatcct ggccctcaca attgtcatag
     1981 gatctgcgtg catttcggac cggcagggta tttcggaaac gagcctccaa ggagaaaaag
     2041 ggagctggct gatgtcctcg ttactttatg tcagctataa cggtacactg gcactggtgg
     2101 tcctttcatc cctgggcagc tatactgata gagtcggagc ggccttggga ggggcagcca
     2161 tcggcgcgct gggaatcatg ttaatggctg gcctgatgtg gtttataacc tgggtaaatt
     2221 accccgggct ttacggagca caggttcccc tgctccaggt ggccaggccc ctgggcaact
     2281 tattatttct gtcatcggtt atagtgctgc tggcagctat gattactacg gccttgggcc
     2341 ttggttttgc ctttgcaagg agcgtttcaa aaagattcgg tctgagttac aaaagggcaa
     2401 tttatttttt gcttatcgga ataccgctgg cgaaatacaa ctttgctgct ttaataaata
     2461 cgatatatcc cttttttgga aaactgggga ttatttttgc catttttttg gcaataaagc
     2521 aggtttttaa acatgaaaat agaatgatat aagtataaat gcagactaac agagcctggg
     2581 gtgacaaaat gaaggaaagg tctgccttaa ggccgtcgat ctactgcggg cttttacttt
     2641 tgctggtact ggccattact ttgcttgtca acaataagcc catcggctat tcaaaaaatg
     2701 cccttttcca gaccctgcag ccaaagatcc ttctcgaagt gcagggggac cttgcttggg
     2761 cagggggcga ttcctattat aactatagcg atggcagcct gaaaatgatg gaggaaacgg
     2821 ggaaagttct gtgggacaca aaaataaagg ggcagcttct ctggattgga gaagaaggag
     2881 taatcgccgc acaggataat atgctgctga tgctggacgg caatggccat gaaatctttc
     2941 aaaaagcgga tttgccggat aagcctaggg tattgtgtgt ccaaggcaaa tacttgcttt
     3001 tgtcgggaaa actatcaggt gtggaacatg gcatattgct gcatgatcgg ggagacatcg
     3061 tgtggcagat tccccttgat ggaagtatta tctcgggcag ccttcatcct aagggaattt
     3121 atgcggtttt cagcgtaatt gacaatgacg caaagtcaag gctcgttgtc gttgggccaa
     3181 ggggagatat tttgatagac agcgggcatc agcttcccct ttaccgaata agagcggtat
     3241 cccagggcat ttgtgtcata actgctgaca gggcatattt aatggattat gatggacggc
     3301 tgctgtggga atatgggttc caggggcagg tattgcgggg agatattggc agcgatgggt
     3361 ctgttgcggc tattgtggaa gagcatgccg ggagcttaag ccaggacctt catcaggtgc
     3421 tcatcatgct atcggagcag gggaggcata tatgctcgta cagccttgat agtcgaccga
     3481 ataatgtcaa agtagacaaa gaccgcgtgt atattgtgga cgattacggg attatgctgt
     3541 tatcccgtga aggtctcttg ctatcgaaga taacgcaaag agggataaaa aagttgacct
     3601 ttacggataa aaataatatt atagtaaatc ttgaagcaag tagtctgctg ctggaaagtc
     3661 caactggggg gatctgaaat gaactggctg gatatcgtcc tgctgataat cataatcaaa
     3721 agcgccctgc agggctattt taagggtttt gtccttgcag cttttaaaac ggcaggtgtt
     3781 atcgtatcat tgtttatggg catattttac agggatgaag cggtgagttt tttgaaggcc
     3841 cagcttgcaa tggataggca cataagcagc atgatgctca gcccggccat gagggattta
     3901 aacacgctgg gagtagctaa tatcagaaac atcatcgatc tcatgctggc tgccgtaggc
     3961 tttttgttga tttttttact ggcccaggtt atttttctag tgccggccta cttgataagc
     4021 ggcatagtaa aactatcaaa cttgtccttg tttgataggc tgctgggagg cctgtttggc
     4081 attgccaggg cagcgctgtg gctggcccta ttaagtgccg tcacatcgcc ttttctactc
     4141 ctttttccgg gcagcatcct ggacagggga atcagctcat cgtatattct aaatcatctt
     4201 aggtttttag actttataag tcccattgtg gtaaaattaa tctaaaaaag gggtgttgga
     4261 tttgggagaa ctggtccaaa catacaacaa tttgataaat caactgccaa catctgcaaa
     4321 atcgctggtc agaatcgccg ttgtcctgct tgtgtcggca ttaatcataa agctggccaa
     4381 cgagtttatc acccgttttt tgtttatgcc taatagggat agaataaaaa ttgacgaaaa
     4441 caagaagaaa accctggcca gccttttaaa gagcatcgta aggtacgtta catatttcat
     4501 ggctgccatg aacattttag aaatcttggg gatcaacact acatctctgc tcacggcggc
     4561 aggtgtcgga ggtcttgcgg tgggcttcgg agcccaaaac ctggtaaagg acattatatc
     4621 ggggtttttt ctgatttttg aagaccagta caatgtaggt gattatgtgg aacttgccgg
     4681 agtttcagga atcgtggatg aaatcgggct taggaccacc aaggtgcggg atttcggagg
     4741 ccagtttcac atcatcccca acggggaaat aaagctggtg accaaccact ccaggggcat
     4801 gaagcgggcc ctggtaaaca taaacatcgc ctatgaggaa gacttaaaca aggttttgaa
     4861 ggcactggaa gaggtctgcc agcaggtaaa ggaaaaacga gaagacattt tggaaggtcc
     4921 gagcgtttta ggaatatcca atttgggtcc aagcgaagtt gtagtgacta ttttggccag
     4981 gaccgagcca atgcagcagt ggagtgtgga aagggaaatt cggaaggcgg ttttagaaaa
     5041 gtttggcaaa gaaggtatag aaatacctta tcagcgcatt gtgtacatta acaaacaaag
     5101 taagggggaa gtccaatgaa ctacgaatta ggtgacatag ttcagatgaa gaaaaagcac
     5161 ccctgcggca gtgacaagtg gcagatttgg agactgggca tggacattgg gataaagtgc
     5221 atggagtgcg ggcgaaaagt gatgataccc cgccgcaaat ttgaaaaatc cgtaaaaaag
     5281 attttaactt cagaacaaag tcccccgtct aataacctgt aagaatgtcg tctttaaata
     5341 taatgatgtt gtccatcttt ttattaaagt tcccggaggc ttgaaaacaa tttgcataat
     5401 tcattgtaag tactttcggg attttttctg tttggatgaa gccaatttat cattatccca
     5461 tgtatccaca aaatctataa aaagggattc cgcaaaaatt gcacataaaa aagcggaaat
     5521 ttgactcatc tccttgtctt gaaaacatat ttgattaatg atataatacc gtcgaacatc
     5581 tctgctccga aaaacggggc caatttgtcc gtggaggtga agaaaagtga gaaagtacga
     5641 gaccattttc atcgttgacc caaattttga agccgatcag gtaaacgagt tggtggaaaa
     5701 atttaagggg ctgatccaag aacagggcgg tcagatagat ggggtcgatg actggggcaa
     5761 gcgcaggctg gcctatccca tcaataaaca gcgggaaggc tattattttt taatcaattt
     5821 cactgcaaag cctgaaaccg cccaggatct tgaaagggtc tacaaaatca ccaatggtgt
     5881 tttgagatac ctcattgtga aaaacgaaaa ataagaggtg aaaaccttgc tgaacaggat
     5941 tgtattgata ggcaggctga cgagggatcc ggaactgaga tttacacctg ccaacggagt
     6001 gccggtcacc cagtttacca tcgcggtaga taggccctat gtgaatcaaa agggtgagcg
     6061 ggaagctgat tttatcagga tcgtcacttg gcgcaaattg gcggaagtct gtgccaataa
     6121 cctcacgaaa gggcgcttgg taggggttga cggcagattg caaataaggt cttatgaagg
     6181 tcaggatggt cagcgaaggt atataactga agttgtggcc gattcagtgc agtctttaga
     6241 caggccgaaa tccggatccg aggattcaag ttttgacagt atggatgcgg actttagttt
     6301 cgatccgggt atagatgatc aaatagaggg tgacggagta cccttctaac tgaaaggagg
     6361 caattgacgc ttgaaaccga aacgagtaaa gaaaaaaaag gtatgcagct tttgcgttga
     6421 taaaatagag tttgttgact ataaagatca cggccgcttg agaaagtata taaccgaacg
     6481 tggcaagatc ctgccaaggc gcataacagg caattgcgct aaacatcaga gacaacttac
     6541 cacagcaatt aagagagcga gaaatatcgc cctgctaccg tttacagcag attagagagg
     6601 gtcttccctc tcttttttgc cataaaggag gaaatactgc atgaagccaa caaacaccaa
     6661 agcactggtg gaaggagcgc tgctggcagc tatcaacata gtgctgagca tcatggccct
     6721 gtacatgccg atccttgggt ctttcgccac tctggtatgg ccggtgccga tcgtcattct
     6781 gggaataagg cacggcttaa ggacaagtat ccttgccacg gcagttgcgg gaattatcgt
     6841 tgcgatattg agcggcccct ttcaggccct gagcattgtc ataagttttg gcttgatagg
     6901 aatagccatg ggtggggcta taaataaaaa gctgtctccc tttaaggtgc tggcagtagg
     6961 tggtgcagct tcactaatat ccaaggtcat actgatcttc ttaaccatgc tgatgatggg
     7021 cataaaccct attaccgaag aaatcaatat aatgaaagaa tccttgctaa tggctggaag
     7081 cctgtatgaa aaatttggca tggaccccca gaccgtagaa actacaatcg atagtttcaa
     7141 taggagcctt gaacttttgc ccatactcat cccgggcatc tttataatgg catctgtaat
     7201 ggatactttt ctgacttatg tagtgacaaa agccgtgctg gcaagaatgg gtcagaaact
     7261 agaagatttg acgccttttt ggctttggaa gtttccggat ttcaccgtgg cattttttct
     7321 tttgggctat ctgctggtgc tcttagagac ctattggccg gtaggggtgc tgaaagccgt
     7381 aggcatgaac atatccttgg tatttggttt tgccttttac atagagggtt tttcgctact
     7441 agcctatttc ctgggcaagt acaatgtggc aaaaccattg agagtactca ttaccttttt
     7501 tgccctcttt aatcccctgt ttttgcagtt gatgtttttt gcgggcttat tcgatattct
     7561 ctttaatttc cgcaagattt attgatttga ggtatttatc atggacagag gaaatgatag
     7621 gataatactg ctgggtggct tgctttacct gctgggagtt atacttatct gcacaattct
     7681 tatagatgtt aaagtggccc ttttgcttct tcccctatta ggcctgctgc tgtactactt
     7741 agtatttgtc aaaaaggaaa tacaatcact gccagcactg atcgaaccca cgaccagctt
     7801 ccggcagggg ctgtggaaaa acactttaaa tgagctgggc acagccgtgg ccatagtaga
     7861 taagaacaac aaggtcatat ggaaaaacaa agtttttatt gacaatttcc aggcggccga
     7921 cgacaggata aaggatatag gtgacattat ccccccagac attttaaact cgcttaatga
     7981 ccaatgccag gagatcgaga taaatgaaag gcacttttcc ctaaggagcg cccccttgcc
     8041 cggcagtcga aggggcaata ggggaatgta cagggcgatt tttatccagg atattgagga
     8101 cctgcatgag
//
LOCUS       DAONUO010000050         8041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6808_length_8041_cov_4.115702, whole genome shotgun sequence.
ACCESSION   DAONUO010000050 DAONUO010000000
VERSION     DAONUO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6808_length_8041_cov_4.115702"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..536
                     /gene="rplU"
                     /locus_tag="PK768_02895"
     CDS             225..536
                     /gene="rplU"
                     /locus_tag="PK768_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQA59951.1"
                     /translation="MYAIIETGGKQYRVSEGDVVRVEKLPGEVGESVEFTKVLAVSKD
                     GQLNVGKPWLEGSKVTAKVLQHGKADKIIVFKYKPKKNYRKKQGHRQPFTEVQIEKIE
                     G"
     gene            538..873
                     /locus_tag="PK768_02900"
     CDS             538..873
                     /locus_tag="PK768_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HQA59952.1"
                     /translation="MIRVRAKRHPQGGYELLEISGHAKYAEYGKDIVCAGVSALAETA
                     VLGVEHVACIKPILKKRQGYFLLKLPDDVEVEALKKAAIILDTIFLGLRDISESYPSN
                     VRIELIEEV"
     gene            877..1146
                     /gene="rpmA"
                     /locus_tag="PK768_02905"
     CDS             877..1146
                     /gene="rpmA"
                     /locus_tag="PK768_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQA59953.1"
                     /translation="MNLQLFAQKKGGGSSKNGRDSQAKRLGVKRFDGQFVSAGSIIVR
                     QRGTRVYPGQNVGMGVDHTLFAKIDGYVKFEQKGKNKKLVSVVAN"
     gene            1335..1928
                     /locus_tag="PK768_02910"
     CDS             1335..1928
                     /locus_tag="PK768_02910"
                     /inference="COORDINATES: protein motif:HMM:NF026040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HQA59954.1"
                     /translation="MKRKVLFFIFLTGQATLTVWAIILSTHIWKIHFILMGLFFYVAS
                     LIMSINFDLNHHATLNVVNRFINVSRSMRHEFRNHLQVLFSMIQLKKYQDALKYIQDV
                     VNSDNTINHIFSNLSDPLLICCLLEIIYSFRQKDIDITVEVLGAPRLPQLWIVKRKMG
                     KYISQFNKIQGKKDVKIILKNSQVEVLSNALGQNIMP"
     gene            2040..3302
                     /gene="obgE"
                     /locus_tag="PK768_02915"
     CDS             2040..3302
                     /gene="obgE"
                     /locus_tag="PK768_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQA59955.1"
                     /translation="MFIDRAKIYVKGGDGGNGVVAFRREKYVPRGGPSGGDGGKGADV
                     VLVVDSSLSTLIDFKYKVHYKGKRGEHGQGSNRFGRSAEDLVIKVPPGTVVKDAESGE
                     ILADLVEDGDRFVAAKGGRGGRGNARFVSSVHRAPDFAEKGEPGEERWIILELKLIAD
                     VGLIGFPNAGKSTLLSRMTAARPKIADYPFTTLSPNLGVVETGHGSSFVLADIPGLIE
                     GAHEGQGLGHEFLRHIERTRLLVHVLDMSGLERDPLEAFFAINDELVKYSDKLLDKPQ
                     IVAANKMDLPEARENYPRVKETLESAGYDVIPVSGATGEGMKELKNRISEALSKIPKT
                     IAIEQVKQYGIEEEEPFKIEKRGDIYYISGKRVERMVAMTDLDNDSAVKRLQLSFKRM
                     GIDDALKEQGINPGDTVVIGDFEFYYVE"
     gene            3397..4011
                     /gene="nadD"
                     /locus_tag="PK768_02920"
     CDS             3397..4011
                     /gene="nadD"
                     /locus_tag="PK768_02920"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQA59956.1"
                     /translation="MSKIKKVGIMGGTFDPIHFGHLVTAEAARTQFQLDKVIFTPTGK
                     PPHKKGYSVTPAEHRYLMTMLAINDNPFFEISRMEIDRPGLTYTVDTLESFYKELGED
                     VKLFFISGADAVLDILTWKDVDRVLSYCTFIAATRPGYPMDKLNEKISELKRMYGWEV
                     YPMKVTGMDISSTDIRRRVREGLSVRYLLPKSVETYIQKNNLYR"
     gene            4017..4595
                     /gene="yqeK"
                     /locus_tag="PK768_02925"
     CDS             4017..4595
                     /gene="yqeK"
                     /locus_tag="PK768_02925"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HQA59957.1"
                     /translation="MLEEIMHKLKANLDEERFKHSLGVMKTAVKLAKHHKADIKKAEI
                     AGLLHDCAKCMDSVSQLKIAENFGILQDDIMRRERAIIHGPVGAILAKRDYGISDEEI
                     LNAIRIHTTGDVNMSLLEKIIFLSDLIEPGRAFPGVDELRVKAFKDLDEAMVHAFDMT
                     IMHVLNKGSLLHPKTVSARNYILMEKESEGKS"
     gene            4592..5770
                     /locus_tag="PK768_02930"
     CDS             4592..5770
                     /locus_tag="PK768_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HQA59958.1"
                     /translation="MKRPLKIALLILLFITISMGSGFYYVFRSLNDPLDPSIDPNVSD
                     STDEYVKGGKQNILVLGMDAGTIGAKEEYNRHRTDTIIVVSLDRKNKDVKVLSIPRDT
                     RVKIGGYGIQKINAAMAFGGPDLAVKTVKDFLGVPIHNYVAIKLSSFRNIVDALGGVE
                     IDVKQRMKYSDPAGGIYIDLYPGLQVLDGDKAEQFVRFREYPDADIGRIRAQQQFMEA
                     LVKQALKPATILKIPKIIEVVQENVETDLQPLELASLANFARQIKQEDIKMYMIPGEG
                     KYISGISYFIPYQEQMNEMIDEVFFDDGSIKVAVLNGNGQQGVASKVARELESLGFKV
                     VSIANADSFDYETTTIIYPSGKKSDAEKIAEVLTEARMEEGADDSITLPTIIVGKDVN
                     "
     gene            5788..6153
                     /gene="rsfS"
                     /locus_tag="PK768_02935"
     CDS             5788..6153
                     /gene="rsfS"
                     /locus_tag="PK768_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQA59959.1"
                     /translation="MTKDPRECALKAASILEDKKGQDVLVLDIRSIAIFADYFVIATG
                     LSPIHVQALADEVEEKLTEEGYFLRSKVGYKEGKWVLLDFNDVIVHIFTAAEREYYML
                     ERLWADATPIKLSNVSIDS"
     gene            6444..>8041
                     /gene="leuS"
                     /locus_tag="PK768_02940"
     CDS             6444..>8041
                     /gene="leuS"
                     /locus_tag="PK768_02940"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQA59960.1"
                     /translation="MKYDFKNIESKWQKKWEEEGIYKTSEDYEKPKFYCLEMFPYPSG
                     NLHMGHMRNYSIGDVIARFKRMNGYNVLYTMGWDSFGLPAENAAIKHNIHPYKWTWSN
                     IATMRSQLKQLGFSYDWDREVATCHPEYYKWTQWFFLLLYKRGLAYRKKATVNWCPSC
                     STVLANEQVVDGKCERCKSEVTKKELEQWFFRITDYADELLDDLEKLPGWPEKVKTMQ
                     YNWIGRSEGVQFAFTEEKTGEKIEVFTTRPDTVYGVSYIVLAPEHPLVDTLCAGTEYE
                     EPVKRFKDKMARYSEIDRTSTEAEKEGMFIGAYAINPMNDDKVPIWIANYVLMGYGTG
                     AVMGVPAHDQRDFDFAKKYDLPIKVVITPKDKVLQAEEMTEAYVDEGILINSDRFNGI
                     NSPDAITAIGQYMSDKGIGEFKVNFKLRDWLISRQRYWGAPIPIVYCDKCGVVPIPEE
                     ALPVLLPEDIEFKPQGTSPLKDCAEFVNTTCPKCNGPARRETDTMDTFMCSSWYFYRY
                     TDPRNQNAPFDEKKVDYWMPVDQY"
BASE COUNT         2549 a         1400 c         1968 g         2124 t
ORIGIN      
        1 cttaagtcaa taagagacgc ttaaggaaaa gatatatcca gagggaatac ttttttattt
       61 taaaacccat agtatcttga cgcgatcttt gatttaaatt tcattgacag caaagctacc
      121 cttgtgctaa aatagcgtag gtggttaagt gcgccggcca ccgcccggga cgggttcaaa
      181 aaattcatta cctagattcc ggcgagatga aggaggagat aaatatgtat gcaattattg
      241 agaccggcgg taaacaatat cgtgtatccg aaggcgatgt agttcgggtt gaaaagctac
      301 cgggagaagt aggagagtcg gtagaattca ccaaggtact tgctgtttct aaagacggtc
      361 agttaaacgt cggtaagcct tggcttgaag gttcaaaggt gacggcaaag gtgctgcagc
      421 atggtaaggc cgacaagatt atcgtcttta agtacaagcc taaaaagaat taccgcaaga
      481 agcagggtca ccgtcagcct ttcaccgaag tgcaaattga gaaaatagaa ggataatatg
      541 atacgggtca gggcaaagcg ccatccacag ggtggttatg agttattaga gatatcaggc
      601 catgccaagt acgccgaata cggtaaggat attgtctgtg caggtgtttc tgcacttgcc
      661 gaaacagcgg tattgggcgt agagcatgtg gcctgtataa aacccatatt aaaaaagaga
      721 caaggttatt ttttgttgaa gctgccagat gatgtagaag ttgaagcatt aaaaaaggct
      781 gcgattattt tagacaccat atttttaggt ctaagagaca tttccgaatc gtacccgtct
      841 aatgtgcgga tagagctaat agaggaggtg tagtggatga atttacagct tttcgctcag
      901 aaaaaaggtg gaggtagctc taaaaacggt cgcgatagtc aagccaaacg cctcggcgtt
      961 aagaggtttg acggtcagtt tgtcagcgcc ggcagtatta tagttcggca gagaggcacc
     1021 agagtatatc cggggcaaaa tgtaggaatg ggcgttgatc atacactctt tgcgaaaata
     1081 gatggctatg taaagtttga acagaagggt aagaacaaaa aactcgtaag tgttgtagca
     1141 aactaagctc ctcttttcag ggagcttttc tttttgccgt ccagttggaa aaaattgtct
     1201 agatttcttt ttttgagaag gaattcagca gaagacatag aatatatata caaatatata
     1261 caaaactaaa aagtaaaatt ctttttacaa tgccaggatt ctcttatatt attgaaataa
     1321 aggaagaggt ggccttgaag agaaaagttt tgttttttat ctttttaact ggacaggcaa
     1381 ctttaacagt atgggcaata atactatcaa cgcatatttg gaaaatacac tttatattga
     1441 tggggctctt tttttacgtg gctagcctca taatgtctat aaattttgat ttaaatcatc
     1501 atgcgacact aaatgtagta aacaggttta taaatgtttc ccggtcaatg agacatgaat
     1561 ttcgaaacca tttgcaggta ttatttagca tgattcagtt gaaaaaatac caggatgctt
     1621 taaaatatat acaagatgtg gtaaatagtg ataatactat caatcacatt ttcagcaatt
     1681 taagcgatcc cttgctgatt tgctgtttgt tggagattat ttacagtttt aggcaaaagg
     1741 atatagatat taccgtggag gtcttaggtg ccccacgctt gcctcaattg tggattgtta
     1801 aaagaaaaat gggaaaatac atctcgcagt ttaacaaaat tcaaggaaaa aaggatgtta
     1861 aaatcatttt aaagaattct caagttgaag ttctttctaa tgcattgggg cagaacatta
     1921 tgccttaaca cacatacctt ttgcaaaaac ctttctgtga ctactgggca aggtttattt
     1981 tttatttgct aaatgggaat ctattaatag tgagaaaaaa tttaagatgg gtggtagaaa
     2041 tgttcatcga tagagcaaag atttatgtaa aaggcggaga tggaggcaac ggagtggtgg
     2101 cttttcgaag ggagaaatat gtaccccgcg gaggtcctag cggtggtgat ggaggcaagg
     2161 gagcagatgt ggtcctcgtg gtggactcat ccttgtctac cttgatagat tttaaatata
     2221 aggttcatta caaagggaaa agaggagaac acggtcaagg gtccaacaga tttggccgca
     2281 gtgctgaaga tttggtcatt aaagttccgc cgggtaccgt tgttaaagat gccgaaagcg
     2341 gagagatttt ggcagattta gtggaagatg gagatagatt tgtcgctgcc aaaggaggca
     2401 ggggcggacg gggcaatgcc cgattcgtct cttcggttca cagggcgccg gattttgcag
     2461 aaaagggtga gccgggagag gaacggtgga tcatcctgga actaaagtta atagcggatg
     2521 ttggcctgat aggctttccc aatgccggca aatccacgct tctttcacgg atgacggcag
     2581 caaggcctaa aatagccgac tatcccttca ccacactatc gccgaacctc ggcgttgtag
     2641 aaactggcca tggcagtagc tttgttttgg cagatatccc cggcctcatt gaaggcgccc
     2701 atgaaggcca aggattaggc catgagtttt tacgacatat tgaaaggacg cgattacttg
     2761 tacatgtact ggatatgtca ggtttggaaa gggacccgct ggaggccttt tttgcaataa
     2821 atgatgaact ggtaaagtac agcgacaagc ttttggataa accccaaatt gtggctgcca
     2881 acaaaatgga cttgccggaa gccagagaaa actacccaag agttaaagag acgcttgaaa
     2941 gtgcagggta tgatgtaata ccggtttcgg gggcaactgg ggagggaatg aaagagctta
     3001 aaaatcgcat aagcgaagcg ctttcaaaga taccgaaaac catagctata gaacaagtta
     3061 agcaatacgg aattgaagag gaagaacctt tcaagataga aaagaggggc gacatttact
     3121 atatttcagg caagagggta gagcgaatgg tggcgatgac ggaccttgac aatgattcgg
     3181 cagtaaagcg gctgcagctt agtttcaagc ggatggggat tgatgatgca ttaaaagagc
     3241 agggcataaa tcccggcgac acagtagtta ttggcgactt tgaattctac tatgtggaat
     3301 aaacaagctt gaatccaaaa tcttgtaatt gaaactgcct tgtcaattat gatataattt
     3361 tttgtaattt aaggagtgta gagggaggat tttgtcttgt cgaaaattaa aaaggtgggt
     3421 attatgggtg ggacttttga tcccattcac tttggccatc ttgttactgc cgaggcggcc
     3481 cgaacccagt ttcagctgga caaggttatc tttacaccga ccggaaagcc gccccataag
     3541 aaaggatatt cagtgacgcc tgcggaacat agatatctaa tgaccatgct tgctataaac
     3601 gacaatccgt ttttcgagat atcgagaatg gaaattgaca ggccgggttt aacctatacg
     3661 gtggatacat tagaaagctt ttataaggaa ttaggagaag atgtaaaact tttttttatt
     3721 agcggggccg atgccgttct tgatatactg acatggaaag acgtggatag ggtgttaagc
     3781 tactgtactt ttatagcggc aaccaggccc ggttatccga tggacaagtt aaacgaaaaa
     3841 ataagtgaac taaaaagaat gtatggttgg gaagtttacc caatgaaagt taccggtatg
     3901 gatatttctt ctacggatat acgtagaaga gttcgggaag gactttcggt tagatatttg
     3961 cttccaaaaa gcgtggaaac atacattcaa aagaacaatc tgtatcggtg aaaaccatgt
     4021 tagaagagat aatgcataag ctgaaagcta atcttgatga agaaagattt aagcattctt
     4081 tgggtgttat gaaaactgct gtaaaattag ctaaacacca caaagcggat atcaaaaaag
     4141 ctgaaatagc aggcctttta catgattgtg caaagtgcat ggactctgtc agtcagttga
     4201 aaatagcaga aaattttggt atacttcaag atgatatcat gagacgggaa agagcgataa
     4261 tccatggtcc agtaggtgcc atcttggcta aacgagatta cggcataagc gatgaggaaa
     4321 tattaaatgc cataagaatc catactaccg gcgatgtaaa tatgagcctg ttggagaaaa
     4381 taattttcct ttccgaccta atcgaacccg gaagagcttt tccgggagtt gatgagctaa
     4441 gggtaaaggc ctttaaagat ttagacgagg caatggttca tgcatttgat atgacaatca
     4501 tgcatgtgtt aaacaaggga agtctcttgc atccaaagac ggtttcagca aggaattata
     4561 ttctcatgga aaaagagagc gaggggaagt catgaaaagg cctttaaaaa tagccttatt
     4621 aattttgttg tttattacaa tttcaatggg aagcggattt tactatgtat tccggagcct
     4681 aaatgaccca ttagatccaa gtatagatcc aaatgtttct gatagtaccg atgagtatgt
     4741 aaaaggcggc aagcaaaata tattagttct aggtatggat gctggaacca taggggccaa
     4801 ggaagaatat aatcgccata gaaccgatac aattattgta gtcagtttag atagaaagaa
     4861 taaggatgtc aaagtcctat ccatccccag ggataccagg gttaagattg ggggatatgg
     4921 cattcagaaa ataaatgctg ccatggcttt tggaggaccg gatcttgcag ttaaaaccgt
     4981 caaggatttt cttggtgttc ccatacacaa ttatgtggca attaaacttt cgagctttag
     5041 gaacatagtc gatgcccttg gtggcgttga aatagatgta aaacagcgga tgaagtacag
     5101 tgaccccgct ggaggcatat atatagactt atatcccgga ttacaagtgc tagatggcga
     5161 caaagctgaa caatttgtga gatttaggga atatccggat gccgatatag gccgcataag
     5221 agcacagcag cagtttatgg aggctctggt taagcaagct ctcaaaccag ctactatttt
     5281 gaagatacct aaaattatcg aagtagttca agaaaatgtg gaaaccgatc tgcagccttt
     5341 agagctagcc agtcttgcca attttgccag gcagataaaa caggaagaca taaaaatgta
     5401 catgattccc ggtgagggga aatacatttc gggaataagt tattttattc cttaccaaga
     5461 acagatgaat gagatgatag atgaggtctt ctttgatgat ggcagcatca aagttgccgt
     5521 tttaaacggc aacggtcagc aaggtgttgc ctctaaggtg gcaagggagc tggaatccct
     5581 cggctttaag gtggtaagta tagcaaatgc tgacagcttc gattacgaaa ctacaacaat
     5641 aatatacccc agcggcaaaa aaagtgatgc tgaaaagatt gctgaggtgc tcaccgaagc
     5701 ccgtatggag gaaggagcag acgacagcat tacccttcct acaattatcg ttggaaagga
     5761 tgtaaattaa ttcatggagg gaaatggatg acaaaagacc ctagagagtg cgctcttaag
     5821 gcggcaagca ttttagaaga taaaaagggt caggacgtat tagtgcttga tatcaggtcc
     5881 atagccattt ttgctgatta tttcgtgata gctaccggct tatcaccgat tcatgttcaa
     5941 gctctggccg atgaggtaga agagaaactt acggaagaag gctattttct ccgaagtaaa
     6001 gtaggctata aagaaggcaa atgggttttg ctggatttta acgatgtaat agtgcatata
     6061 tttaccgctg ctgaaagaga atattatatg ttggagagac tctgggcaga tgcaacacct
     6121 attaagcttt ccaatgtgtc tattgactct taaaaaaact tagaatataa ttaacaggtc
     6181 tatataacaa gttcatatgt tacaaaggag tagtaagggt cgtattgttc tttacagaga
     6241 accgggcaaa ggtgagagcc ggaagagcaa ttccctgaac tcgcctttaa attgtgctga
     6301 caagcgttaa aatgttagag tcgggcctaa tataaattct ttaggccaat tagggtggta
     6361 ccgcgagaaa gtttctcgtc cctaacaggg attggaaact tttttttaat atctgaacca
     6421 tgtaaatgga ggaggcaacc aagttgaaat acgatttcaa aaatattgaa agcaagtggc
     6481 agaaaaagtg ggaagaagaa ggaatatata aaacatcgga agactatgaa aagcctaaat
     6541 tctattgcct tgaaatgttt ccttaccctt ccggaaatct tcacatgggg cacatgcgca
     6601 attattcaat aggcgatgta attgcgcgat ttaaaagaat gaacggttat aacgtgcttt
     6661 atactatggg ctgggattcc ttcggcctgc ctgcggaaaa cgcagccata aagcacaata
     6721 ttcatccgta caagtggacc tggagcaata ttgcaactat gaggtcacag cttaaacagc
     6781 taggttttag ttatgactgg gacagggagg tagctacatg ccatcctgaa tattataaat
     6841 ggacccagtg gttctttctc ctattgtata aaaggggcct ggcttacaga aagaaagcta
     6901 ccgtaaactg gtgcccatcc tgttcaacgg ttttggctaa cgagcaggta gtcgatggca
     6961 aatgcgagcg ttgcaaatct gaagttacta aaaaagagct ggagcaatgg tttttcagga
     7021 ttacggacta cgccgatgag ctattagatg atttggagaa gcttcccggt tggcctgaaa
     7081 aagttaagac catgcagtac aactggatcg ggcgaagcga aggtgtacag tttgccttta
     7141 ccgaggaaaa aaccggagaa aagatagaag tgtttaccac acggccggac acggtctacg
     7201 gcgtttcata tatcgtttta gctcccgaac atcctttagt ggacactctt tgtgccggaa
     7261 ctgagtatga agagccggtg aagagattta aagacaagat ggccaggtac agtgaaatcg
     7321 acagaacttc caccgaagct gaaaaggaag gcatgtttat tggcgcttat gccataaacc
     7381 ccatgaatga tgacaaagtt cccatttgga tagccaacta tgtattaatg ggatacggta
     7441 ccggtgccgt aatgggggtt cctgcccatg accagcggga ctttgatttt gcgaaaaaat
     7501 acgatttgcc cataaaggtt gtcataactc ccaaggataa agttttgcaa gctgaggaaa
     7561 tgaccgaagc ttatgttgac gagggaatac tcattaactc agacagattc aatggcataa
     7621 atagtcctga tgccatcacg gcaataggtc aatacatgag tgataagggc attggtgagt
     7681 ttaaggttaa ctttaagctc agggactggc tcatatcgag acagagatat tggggcgctc
     7741 caattcccat agtctactgc gataagtgcg gcgtcgttcc gatacctgaa gaggccctgc
     7801 cggtactttt gcccgaagat atagaattta agccgcaggg gacttcgccc cttaaagatt
     7861 gtgccgagtt tgtaaacaca acttgtccta aatgtaacgg tcccgctcgt agagaaacgg
     7921 ataccatgga cacctttatg tgttcttcct ggtattttta cagatacact gaccctagga
     7981 atcaaaatgc gccctttgat gaaaaaaagg tagattactg gatgcccgtg gaccagtata
     8041 t
//
LOCUS       DAONUO010000051         8022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6833_length_8022_cov_4.322079, whole genome shotgun sequence.
ACCESSION   DAONUO010000051 DAONUO010000000
VERSION     DAONUO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6833_length_8022_cov_4.322079"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2590
                     /gene="recJ"
                     /locus_tag="PK768_02945"
     CDS             <1..2590
                     /gene="recJ"
                     /locus_tag="PK768_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQA59961.1"
                     /translation="KTNIISRFEPEDKMIYGNYKWQIDSRQEPAETYGNLHPIILQLL
                     KKRGVVTPEQIQRFFYPKLDYMYNPLLLKDMDKALARIKEALARGERILVYGDYDVDG
                     ITSSAILVKLLRKLGGYVDYYIPSRINEGYGLNKEAIDMISKDGIKLIITVDNGISSY
                     EEVKYAASLGIDTIITDHHEPQEQIPEAVAVINAKQRECSYPFKELAGVGVALKLAQA
                     LIGKDCYPELQQEFLELTAVGTLADMVLLLYENRIIVKNGLKNLANTNNKGLSAMMTL
                     LNIKDNPVEAEKISYLLAPRINAAGRIADPKIAVELLLSEDDCRAFELATELEKINQE
                     RQALEAKVLDEAKEMIHKRIDLDSDSIIVISSPNWHPGVIGTVASKLVEIYDRPCIVI
                     AEYRGEGRGSGRSVSGFNLFKAVSELSQLLTKYGGHEQAVGLNIKLENIDVFRDRINQ
                     FNGHTFKNVDFRPKLDIDLELSQRDISLELAQQLEQMKPFGYGNPKPLFICRNLHIRD
                     SRTVGNGDKHLKLNLTSYEEENIDAIGFNFGLYKEDLEAASIMDAAFSLEVNRWKGFV
                     GLQLNIKDLRVPFLQDELLDEIEQNYYRHFVSNLNNKDSVLSKRKEVDKDNIFVQDNL
                     SLLEKINYVKKIFETSERFIVLVNTPYKAWQLLTNLKSNHIFEEEIGVFYNLSSATNK
                     DLSKIILVDPFAENLNKGYYDIVLFDAPFSIDLLNKQLTNSPSTSKVHILFERGDLRY
                     NFLVCRQILPSINQLKAVYNALGKLTSGKFTGSFKHDQLLDLLRGQYGMDMHFVTLIN
                     VFNIYLELKILDFILEDEILHITAFLKHNSTFRLELSQTYKSLYLLKKYVLDFYNSFS
                     TIQFKE"
     gene            2604..3116
                     /locus_tag="PK768_02950"
     CDS             2604..3116
                     /locus_tag="PK768_02950"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HQA59962.1"
                     /translation="MDLKEKIRVIEDFPKKGISFKDITTLIKDGEAFKEAIKSMAELI
                     RDKEVDLITGPEARGFIIGAPLAYELGVGFIPTRKKGKLPGKTIKAEYQLEYGSDIIE
                     MHEDAIKAGQKVVVVDDLLATGGTIISTIELVEKLGGKVAAVVFLIELEDLKGREQLS
                     DYEVYSLVKY"
     gene            3138..5303
                     /locus_tag="PK768_02955"
     CDS             3138..5303
                     /locus_tag="PK768_02955"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQA59963.1"
                     /translation="MEFAVLEKKILSYSPSADINLIRKAYEFAKKAHSGQLRVSGELF
                     IFHPLEVAKILADLELDVTTIAAGLLHDVVEDTEYTLDDIESNFGSEVALLVDGVTKL
                     GKLEYKTKEEQQAENLRKMFFAMAKDIRIILIKLADRLHNMRTLKSMPKDKQREKALE
                     TVEIFAPLAHRLGISKVKWELEDLAFRFLEPERYYELVEKVAKKRQERENHINEMISI
                     LKERLAASGITAEIQGRPKHFFSIYKKMKDQNKTFDQIYDLTAVRVIVNTVRDCYGAL
                     GVVHTLWKPIPGRFKDYVAMPKPNMYQSLHTTVIDSSGDPLEIQIRTFEMHKTAEYGI
                     AAHWRYKEGNKNNSEFEQKLSWLREILDWQRDLRDAKDFMETLKIDLFTDEVYVFTPK
                     GDVIDLPKGSCPIDFAYRIHTDIGNRCVGAKVNGKMVPLDYELANGDIVEIITSAHSN
                     GPSRDWLNIVKSPQAKNKIRQWFKKERRDENIARGKEMLEKEARRNGYDIYQLIKHDF
                     MNHIFKRMSFTSIDDMYAAIGYGAVTTKQILQRILDEFKKNVQPDKSELSLEKADSKS
                     KIKKKDERGIKIDGIGNLLVKFSRCCNPVPGDKIIGYVTRGRGVSIHRKDCKNLNQGN
                     IDQERLIEVKWEGFEDTSYPVEIQVWAYDRQGLLSEIINLLGDIKTNIDAVNARATKN
                     GVAVIDLILEINNKQHLESVMQKILKIKGIYEVKRVMGM"
     gene            5314..5763
                     /gene="dtd"
                     /locus_tag="PK768_02960"
     CDS             5314..5763
                     /gene="dtd"
                     /locus_tag="PK768_02960"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQA59964.1"
                     /translation="MRAVVQRTKEAFVSVRGSIVGQIDKGLVVFLGVAIDDTMVDVNY
                     MAEKIVGLRIFEDEEGKMNKSLQDVDGALLVVSQFTLLGDARKGRRPSFTQAADPEKA
                     NKLYEAFIEICKNKVKIVEKGQFQAEMLVNVSNDGPVTILLDSKKQF"
     gene            5774..6394
                     /locus_tag="PK768_02965"
     CDS             5774..6394
                     /locus_tag="PK768_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA59965.1"
                     /translation="MFLQQLQVGPLETNCYIIADEKSKIAAIIDPGENAKQIINAVNE
                     AELKVKYIFLTHGHSDHIAALKEIKEATGAKIVIHELDAHMLTSAESNLSFLLGERFT
                     QPSADIKLKGSEEFRLGDLFLEIIHTPGHTPGGISIKVGNVVFTGDTLFAGSIGRTDF
                     PGGSFDMLISSIKEKLLKLDDDTKIFPGHGESSTIGYEKRMNPFLR"
     gene            6466..6678
                     /locus_tag="PK768_02970"
     CDS             6466..6678
                     /locus_tag="PK768_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59966.1"
                     /translation="MARKNRSIKKKLSENEDYSDILDIIGQDDDISDEDVALYANIDI
                     DTVKRIREKWVNEELVNPYKKQPGRN"
     gene            complement(6707..7876)
                     /locus_tag="PK768_02975"
     CDS             complement(6707..7876)
                     /locus_tag="PK768_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQA59967.1"
                     /translation="MQFYRKKYIIAVLLGLALGIALVTGGILAYNSIYPDAAARAGSE
                     QNETSNSTPSGILPTDIPEIVEKVGDAVVYIETVSESSSSSDPFFNDPFFRYFFGDSF
                     GFRTTPRVSRGIGTGFIINPKGYIITNEHVINNATEVTVTVIGFDEPFKATVVGKDFN
                     LDLAVLKIDSSKKLPYIELGNSDEMRVGDWVIAIGNPYRLDHTVTVGVISAKGRPVTI
                     PDQSTGKQRVYKDLIQTDAAINPGNSGGPLISLDGKVIGINTAVNAQAQGIGFAIPIN
                     TAKEVLDELITTGSVTRPFIGVLLQDVTKDLADYFGLEKPKGSIIADVVSNSPAEKAG
                     LQRGDVILKINDTDIKNSNDVSNVVSKSKINDKLVMLILRNGQTQYVTVVVGKQS"
BASE COUNT         2668 a         1334 c         1738 g         2282 t
ORIGIN      
        1 aaaaacaaat atcatttcac gttttgaacc ggaggacaag atgatttatg gcaattataa
       61 atggcagatt gattcaaggc aggaaccggc tgaaacttac ggcaatcttc atcccataat
      121 ccttcagtta cttaaaaaaa gaggggtagt cactcctgaa cagattcaga gattttttta
      181 tcctaagttg gattatatgt ataatccgtt gctgttaaag gatatggata aagccctggc
      241 gcgcattaag gaagcccttg ctagaggaga aagaatactt gtctatggag attacgatgt
      301 cgacggcata actagcagtg ccattcttgt caaattgttg aggaaattag ggggatacgt
      361 tgattattat attccttcaa ggataaacga aggttacgga cttaataaag aagccataga
      421 tatgataagc aaggacggga taaagcttat cataacggtt gacaatggga taagttctta
      481 tgaagaggta aaatatgctg ccagtttggg aatagacact attataaccg accatcatga
      541 accgcaagag cagattcctg aagccgtagc tgtaataaat gccaagcaac gagaatgcag
      601 ttatcctttt aaggaattgg ccggtgtagg cgttgcatta aagctcgctc aggcattgat
      661 tggcaaagat tgttatccag aacttcagca ggagtttttg gagctgacag ctgtaggaac
      721 tttagcagat atggtgctgc ttttatatga aaaccgtata attgttaaaa atggccttaa
      781 aaatttggca aatacaaata ataaaggcct ttcagcaatg atgactttgt tgaatataaa
      841 agataatcct gtggaagcag aaaaaattag ctatttatta gcgccaagaa taaatgcagc
      901 cggaaggatt gcagacccca aaatagctgt ggagctattg ctgtctgaag atgattgcag
      961 ggcatttgaa ctggccacgg aactcgaaaa aatcaatcag gaaagacaag ctttagaggc
     1021 gaaggtgtta gatgaagcaa aagaaatgat tcataaaaga atagatctgg attcagatag
     1081 cataatagtc atatcaagtc ccaattggca tccgggagtt ataggaacgg ttgcgtcaaa
     1141 actggttgaa atttacgata gaccctgtat agtgattgca gaataccgcg gtgaagggcg
     1201 gggttctgga agaagcgttt caggctttaa cctttttaaa gccgtaagtg agctttctca
     1261 attgcttaca aaatatggcg gccacgagca ggcagtaggt ttaaatatta aactggaaaa
     1321 catagatgta tttcgagaca gaataaatca gtttaacggc catactttta aaaatgtaga
     1381 ttttagaccc aaacttgata tagatttaga attgagtcag cgggacatca gtttagaact
     1441 ggcacaacaa cttgagcaga tgaaaccctt tggttacggc aatcctaaac cgctctttat
     1501 atgtagaaat ttacacattc gagattcaag gacggtaggt aatggagaca aacacttgaa
     1561 attaaattta acaagttatg aagaagaaaa tattgatgct attggattta actttggttt
     1621 gtataaggaa gacctggaag cagcttcaat tatggatgca gcctttagcc ttgaagtgaa
     1681 tcgttggaaa ggttttgtag ggctgcagct gaacattaag gatttaagag tgcccttttt
     1741 gcaagatgaa ctgctggatg aaattgaaca aaactattat aggcattttg tttcaaatct
     1801 taacaataaa gatagtgttt taagtaaaag aaaagaagta gataaagaca atatctttgt
     1861 acaagataat ttgagcctcc tggaaaagat aaattatgta aaaaagattt ttgaaactag
     1921 tgaaaggttt atagtactgg ttaacactcc ctataaagct tggcaactgt taacgaatct
     1981 aaaatccaat catattttcg aagaagaaat tggggttttt tataatttaa gctctgctac
     2041 aaataaagat cttagtaaaa ttattctagt cgatcccttt gcagaaaacc taaataaggg
     2101 ttattatgac attgtgcttt tcgatgctcc ttttagcatc gatttgttaa ataagcaact
     2161 tacgaattca ccatctactt caaaagtcca tatacttttt gaacgggggg atttacgata
     2221 taatttttta gtgtgtaggc agattttgcc cagcataaat caactaaaag ccgtttacaa
     2281 tgcccttggc aaacttacat ctggaaaatt taccgggagc tttaaacatg atcaacttct
     2341 ggacctgttg cgtggacaat atgggatgga tatgcacttt gttactttaa taaatgtgtt
     2401 taatatttat ctagagctaa agattcttga tttcatatta gaagatgaaa tattacatat
     2461 aactgctttc ttgaaacaca acagcacttt cagactggaa ctatctcaaa catataagtc
     2521 cctgtatttg ctcaaaaaat atgtactgga tttttataat agtttttcta caatacaatt
     2581 taaagaatga tggaggataa taaatggatt taaaagaaaa gattagagta atcgaggatt
     2641 ttcccaaaaa aggaataagt tttaaagaca ttacgacttt aatcaaagat ggagaagcct
     2701 tcaaggaagc tattaagtct atggcagaac tcataagaga taaggaagtg gatttaatca
     2761 caggtcccga ggctagagga tttattatag gagcacctct tgcgtatgaa cttggtgtcg
     2821 gtttcattcc aaccagaaaa aaaggcaaat tgcctgggaa aaccattaag gcagagtatc
     2881 agttagaata tggttctgat ataatcgaga tgcatgaaga tgctataaag gcaggacaaa
     2941 aagtagtggt ggtagatgat ttacttgcaa ctggtggcac tataatttct acaatagagc
     3001 ttgttgaaaa actaggaggg aaagttgccg cagttgtttt tctaattgaa ttagaagatt
     3061 tgaaaggcag agaacaatta tcggattatg aggtatactc tcttgtgaag tattaaatat
     3121 ggtcaggggt ggaaggtttg gaatttgcag ttttagaaaa gaaaatattg tcatacagtc
     3181 catcagctga cattaactta ataagaaagg cctacgaatt tgcaaagaaa gcacatagtg
     3241 gccagctaag ggtgtcaggg gaattgttca tcttccaccc tctggaagtt gctaaaatat
     3301 tggctgactt agaactggat gtaacgacta ttgctgcagg gctactgcat gatgtggtgg
     3361 aggatacgga atatacatta gatgatatag aaagtaattt cgggtcagaa gtagctcttt
     3421 tagtagatgg tgtcaccaag ctgggtaaac ttgagtataa aaccaaggag gagcagcagg
     3481 ccgaaaacct tcgcaagatg ttttttgcca tggctaagga cattcgcata attctcatta
     3541 agctggcaga taggctgcac aacatgagaa ctcttaaatc catgccaaaa gacaagcaaa
     3601 gggaaaaagc cctggaaact gtcgagatct ttgcacctct tgctcatcgc ttgggaattt
     3661 caaaagtaaa atgggaactt gaagatttag cgtttagatt tcttgagcct gaacgttatt
     3721 atgaacttgt agaaaaggtt gcaaaaaaac gtcaggaaag agaaaaccat ataaacgaaa
     3781 tgataagtat actcaaggaa cggctcgcgg cttccggaat aaccgccgag atacagggca
     3841 ggcccaagca ttttttcagt atttataaaa aaatgaagga ccagaataaa acatttgacc
     3901 aaatatacga tttgactgcc gtaagagtta ttgtaaatac tgtaagggat tgttacggag
     3961 ctctgggagt cgttcacact ttatggaaac ctattcccgg cagatttaaa gattatgtag
     4021 ctatgccaaa acccaatatg tatcaatctc tacacactac ggtaatcgat tcttcaggcg
     4081 accctcttga aattcaaata aggactttcg aaatgcataa aaccgctgag tacggaattg
     4141 cagcgcattg gaggtacaaa gagggcaata aaaataacag tgaatttgaa caaaagcttt
     4201 catggcttag ggaaatcctg gattggcaac gggatttaag ggatgcaaaa gattttatgg
     4261 agaccctgaa aatcgatttg ttcaccgatg aggtatacgt atttacgcca aaaggcgatg
     4321 tcatcgattt gcctaagggg tcatgtccta ttgattttgc atatagaatt cacactgata
     4381 taggaaatcg atgcgtggga gctaaagtaa acgggaaaat ggttccgctg gattatgagc
     4441 tggccaatgg cgatattgta gagattataa cttccgccca cagtaacggt cctagtcgcg
     4501 attggctgaa tatagtaaaa tctccccagg caaagaataa aatcaggcaa tggttcaaaa
     4561 aagaacggcg ggatgaaaat atagccagag gcaaggagat gctggaaaaa gaagcccgca
     4621 gaaacggata tgacatttat cagcttataa aacatgattt tatgaatcac atatttaagc
     4681 gaatgagctt tacaagtata gatgatatgt atgctgcaat aggctatgga gctgtaacaa
     4741 ctaagcaaat acttcaaagg atcttggatg aattcaaaaa aaatgttcag cccgacaagt
     4801 cggagctcag tttagaaaaa gccgatagca aatcaaagat taaaaagaaa gatgaacggg
     4861 gaataaaaat tgatggtatc ggtaatctac tagtaaagtt ttccagatgc tgcaatccgg
     4921 ttccgggcga caaaataatt ggttacgtaa ccagaggcag aggagtatca attcacagaa
     4981 aagactgtaa aaacttgaac cagggtaata ttgatcaaga aaggctcata gaagttaagt
     5041 gggaaggttt tgaagacact tcctatcctg tggaaataca ggtttgggca tatgataggc
     5101 aaggcttact ctctgaaata atcaaccttc ttggagatat taaaactaat atagatgcgg
     5161 ttaacgcaag ggctacaaaa aacggtgttg ctgttattga cttgatttta gaaattaaca
     5221 ataaacagca tttggaaagt gttatgcaaa agatattaaa gattaaagga atttatgaag
     5281 taaagcgtgt tatgggtatg tgaggaggat ttcatgaggg cagtagttca aaggacaaaa
     5341 gaagcttttg tttctgttcg tggaagtatc gttgggcaaa ttgataaggg gctggtagta
     5401 tttttaggtg ttgccattga cgataccatg gttgatgtca attacatggc tgaaaaaata
     5461 gtaggtctta ggatttttga agatgaagag ggaaagatga acaaatccct tcaggatgta
     5521 gatggcgcac tgcttgtcgt atctcaattt actcttttgg gagatgcacg aaaggggcgt
     5581 aggccaagtt ttactcaagc agctgatcct gaaaaggcta ataagcttta tgaagcattt
     5641 atcgaaattt gcaagaacaa ggttaaaata gttgaaaaag gtcagtttca ggctgaaatg
     5701 ttagtcaacg tatcaaatga tggccctgtc accatattgc tggacagcaa aaaacagttt
     5761 taggaggaat tagatgtttt tacagcagct acaagttggc cctttggaaa caaattgtta
     5821 tataattgcc gatgaaaaaa gtaaaattgc tgcaataata gatcccggtg agaatgccaa
     5881 gcagattata aatgcagtga acgaagcgga actcaaagta aaatacattt ttttaactca
     5941 tggtcacagc gatcatatcg ctgctttaaa agaaataaaa gaagctacag gtgcaaaaat
     6001 tgtaattcac gagcttgatg ctcacatgct gacctcagca gagagcaatc tttcattttt
     6061 gttgggtgaa agatttacac agccctcagc cgacataaaa ttaaagggca gtgaagagtt
     6121 tagactaggg gatttatttt tggaaattat tcataccccc ggtcatacac ccggcggcat
     6181 atctatcaaa gttggtaatg ttgtttttac cggagataca ttgtttgcag gttcaattgg
     6241 aagaacggat tttccaggtg gttcctttga tatgcttatt agttcaataa aagaaaagtt
     6301 attaaaacta gatgatgata cgaaaatttt ccccggacac ggtgaatcat ctactatagg
     6361 ttatgaaaaa aggatgaatc cctttttgcg gtaactgccg tagctatata aacatatttt
     6421 ttgtttaaaa ggttaaacta tggacagtgc ggcaggaggg atattatggc caggaaaaac
     6481 agaagcatta aaaaaaagct gagcgagaat gaagattatt cggatatcct tgatattatc
     6541 ggtcaagatg atgacatctc ggacgaagat gttgcccttt atgccaatat agatattgat
     6601 accgtgaaga gaatcaggga aaagtgggtc aatgaagagc ttgtcaatcc atataagaaa
     6661 cagcccggca ggaactaaac tgccgggctg caacttttct ttcatattaa gactgttttc
     6721 caactaccac tgtaacgtat tgagtttggc cgtttctcaa tatcaacatt accaacttat
     6781 cattgatctt ggatttgctg acgacattgc tgacatcatt tgaattcttt atatcggtat
     6841 cgtttatctt taatatcaca tcacctcgtt gtagacctgc tttttccgca ggactattgg
     6901 aaacaacatc agcgataatt gagccctttg gcttttcgag cccaaagtaa tcagccagat
     6961 cttttgttac atcttgaagg agcaccccta taaatggcct ggtcaccgaa ccagttgtta
     7021 taagttcatc cagaacttct tttgctgtat ttatcggtat tgcaaaacca ataccctgag
     7081 cttgggcatt gactgccgta tttataccga ttaccttacc atccagtgat atcaaggggc
     7141 ctccgctgtt gcccggattg atagctgcgt cggtttgtat caagtcctta taaacccttt
     7201 gcttacctgt tgactgatct gggattgtga ccggtcttcc tttggcactt attaccccaa
     7261 cggttaccgt gtggtcgagc ctgtagggat tgcctattgc gataacccaa tcgcctaccc
     7321 gcatctcgtc agaatttccc aattctatat aaggcagttt cttggaagaa tcaattttca
     7381 atacggccaa gtctaaattg aaatctttgc caacaacggt tgctttgaaa ggttcatcaa
     7441 aacctataac tgtaacggta acctctgtgg cgttgtttat aacgtgttcg ttggtgatta
     7501 tatagccttt aggattgatg ataaagcctg taccgatgcc cctgcttacc cttggcgtgg
     7561 tacgaaagcc gaagctatct ccaaaaaagt atctaaaaaa tgggtcattg aagaagggat
     7621 ctgaagaact gctgctttcg ctgactgttt cgatgtagac tactgcatcg ccgaccttct
     7681 ctacgatttc cggtatatct gttggcaaaa taccggacgg tgtcgagttt gacgtttcat
     7741 tttgttcact tcctgcccga gccgccgcat cagggtatat ggagttataa gctaaaatac
     7801 ctccagtaac taaagcgatc ccgagggcta aacccagcag caccgcaata atatattttt
     7861 ttctataaaa ctgcatgctt tcaacctcca aaatcattta ataactatta taattataac
     7921 cgtatttttt attgacttta tgtgtaattt gttatgaatt tgtgaaatca acactccggt
     7981 acattgacac agggagctga tttaattata atagtcaaaa aa
//
LOCUS       DAONUO010000052         8009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6853_length_8009_cov_3.502389, whole genome shotgun sequence.
ACCESSION   DAONUO010000052 DAONUO010000000
VERSION     DAONUO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6853_length_8009_cov_3.502389"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PK768_02980"
     CDS             complement(<1..416)
                     /locus_tag="PK768_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HQA59968.1"
                     /translation="MIKTVIPIKMQKLAFNKSVLQKLAMGLFFFASLSAMMFFSALPQ
                     KYDVEVGQVLQENILARNEAINTIATSKLRQAAADQVPKKYTLNHTITAEVKNNVTDI
                     LNYVKEVRNRTYLEDQEKVKLLMDRVSSQISEESYF"
     gene            complement(367..1410)
                     /locus_tag="PK768_02985"
     CDS             complement(367..1410)
                     /locus_tag="PK768_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQA59969.1"
                     /translation="MTENLAEARVSIDNINDISFLFGNQDSNIKCIEQQFNVRILTRG
                     GEIAIIGEEKDVHSVKELFKQMIQLLQRGSSLTVNEVKYFVTLAKNGKGESINDLYDG
                     IVCYTYRGKIIKPKTIGQKQYISAIKRNDIVFAIGPAGTGKTYLAMAMAVTAFKEKEV
                     NRIILTRPAVEAGEKLGFLPGDLQDKVDPYFRPLYDALYDILGVDSFRRYMEKGLIEV
                     APLAYMRGRTLDDSFVILDEAQNTTSEQMKMFLTRLGFGSKAVVTGDLTQIDLPKGVY
                     SGLEEVQEVLKGVKGIDFVYLDDTDVVRHEVVQRIIKAYERFEKERQQNTGRLRDHDK
                     DSNSDKNAKAGIQ"
     gene            complement(1407..2627)
                     /gene="yqfD"
                     /locus_tag="PK768_02990"
     CDS             complement(1407..2627)
                     /gene="yqfD"
                     /locus_tag="PK768_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HQA59970.1"
                     /translation="MLFIKLWNYLSGYVIIKIVGEYGERLLNQAALENLYLWDIKRQN
                     GNVLSCKVSVKNFAKLASLARKTRCRVYIIERVGLFFVILKLRKRKWFLFGALLFIAA
                     VYFMSSFIWSIEIVCPDDELTISVDEALRSWGIKEGIFKYGLDKEYYIDKLLNEYGGL
                     AWAQMEIRGSRLIIELVKKELPPELEENIPCDIIASKDGIIEEIIALRGEALVEPGQT
                     VSEGDVLITGKIMLGHEPDGEEEGSDTLFVHAEGIVRARVWYQKAVKVPLVKTKKVPT
                     GNSKKSLILHFQNHILNLHLGDISYTLYDKRVLKEVNILPKLGGGLKLNIVEYIEMEA
                     QKEYLGVENASIEAEAQLLSDLEHLSKENKIIQRKMEYVLDSDEQAVIGSMIIELVED
                     IGKKQKIEYGEEKL"
     gene            complement(2646..2915)
                     /gene="yqfC"
                     /locus_tag="PK768_02995"
     CDS             complement(2646..2915)
                     /gene="yqfC"
                     /locus_tag="PK768_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HQA59971.1"
                     /translation="MSEGIKKKISDALDLPQDIVLNVPRVIVTGKIATFIENHKGIIE
                     YNSELVKINTSIGVICVRGEDLHIKTIISDEITVEGKISAIEFEE"
     gene            complement(3097..3507)
                     /locus_tag="PK768_03000"
     CDS             complement(3097..3507)
                     /locus_tag="PK768_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59972.1"
                     /translation="MESIVLLVIFLVFRTVFSHLLETQKTQKHPFPKTPVRPRGYQLP
                     PKQDIKPKTIEIAEPKKAETKADILEIPFEDVDVQNINKLISVKSERMQPESLQQAKS
                     IGEFSELFTQDNILRGMILKEVLLPPKAIRKRYD"
     gene            complement(3512..4516)
                     /gene="floA"
                     /locus_tag="PK768_03005"
     CDS             complement(3512..4516)
                     /gene="floA"
                     /locus_tag="PK768_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778175.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HQA59973.1"
                     /translation="MQALIGLFLTIAVIFIALSVVLSFVPLGLWISALAAGVKIGIFN
                     LVGMRLRRVPPNKIVNPLIKAVKAGLDVSVNKLEAHYLAGGNVDRVINALIAAQRAEI
                     PLSFERAAAIDLAGRNVLEAVQMSVNPKVIETPIVAAVAKDGIEVKAKARVTVRANID
                     RLVGGAGEETIIARVGEGIVTTVGSSESHKEVLENPDSISRTVLSEGLDAGTAFEILS
                     IDIADVDVGRNIGAQLQTDQAEADKRIAQAKAEERRAMAVAREQEMKAMVQEMRAKVV
                     EAEAEVPKAMAEALRQGKLGVMDYYSMKNIIADTQMRDSISRLGQKESEDPGKQDKSR
                     "
     gene            complement(4516..5847)
                     /locus_tag="PK768_03010"
     CDS             complement(4516..5847)
                     /locus_tag="PK768_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQA59974.1"
                     /translation="MRDKRVLILAFIVLFTFAFINDSFGQNIKSSADYIYVIPVKGVI
                     NRGLAAFVTRMIGEAEKAGAKALIFEIDTPGGEVGAAIQMSNAILDTFIPTVSFINND
                     ATSAGVIIAISSKKLVAVPGGTIGAAETRPNEEKYISYWSSALRGVAEKTGRDPDLVA
                     AMADADVVIEGVKEKGKILSLTTGEGLELGLIDEQVTSLEELINKIKERENINNVEVV
                     KADMNLSERIAHMVTNPYVAPILLTLGIVGVITEILTPGFGVPGIIGLISFGLFFGGS
                     YLAGAAKIWVIGLFILGLALLIIEMFIPGFGVFGVTGVLSIIASVIIAFPSLEQALIS
                     ILVALVFSTLLIYFLVKYLGRSLIFDRIILGTKQAKSEGYVASIKDESQLVGAEGKAV
                     TPLRPTGVALIGEKRLDVLTEGEFIPRNSDIIVSRVEGNKIIVKLKNEEGA"
     gene            complement(5972..6415)
                     /locus_tag="PK768_03015"
     CDS             complement(5972..6415)
                     /locus_tag="PK768_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQA59975.1"
                     /translation="MSIKDMLNQDMKAALKAGEKDRLSVIRMAKSAILYAEKEKLHEL
                     NDEEIIGVLFREVKKRKQDMEEYRRLGKEEVAKSLEQEIAILSGYLPQPLTQEELEEI
                     IRQTISEVGANGMKDMGKVMGAVMPKVKGRADGSQVSSLVKKLLQ"
     gene            complement(6427..6609)
                     /gene="rpsU"
                     /locus_tag="PK768_03020"
     CDS             complement(6427..6609)
                     /gene="rpsU"
                     /locus_tag="PK768_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQA59976.1"
                     /translation="MSEVIVGENESLDSALRRFKRKCQRAGVLSEVRKREHYEKPSVR
                     RKKKSEAARKRKFRKF"
     gene            complement(6706..7047)
                     /locus_tag="PK768_03025"
     CDS             complement(6706..7047)
                     /locus_tag="PK768_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQA59977.1"
                     /translation="MDCIFCKIINKEIPAKVAYEDDNVVAFHDINPQAPIHLLIVPKK
                     HITSVMEIHDEENLLLGDIILTAQNLARENNLDKKGFRIVVNTGEEGGQTVNHLHFHL
                     LGGRFLTWPPG"
     gene            complement(7139..>8009)
                     /locus_tag="PK768_03030"
     CDS             complement(7139..>8009)
                     /locus_tag="PK768_03030"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HQA59978.1"
                     /translation="ILKIQEGCNQFCSYCIIPYARGPIRSRAIESIMAEGRSLAKDGF
                     KEIVLTGIHLGLYGFDFKDKKNHLLDVISMIAQIEGIERVRLSSIEALELTDEFLDGL
                     LKIESFCHHFHIPLQSGCDTVLKRMNRRYSTEDFRNRVNYIRKLMPDVSITTDVIVGF
                     PGETEEEFKMTESFISEMEFSKLHVFPFSPRRGTVAADMPDQIEKSIKNERSQRLIQL
                     SQKLERSFREKFLNTTRYVLFEDKKADSKYEGLTENYIKVMVESKENLHNQIKAVKLS
                     KNTDNYIFGEIDE"
BASE COUNT         2091 a         1902 c         1363 g         2653 t
ORIGIN      
        1 ataaaatagg attcctctga tatttgactg gataccctat ccatcagaag ctttactttt
       61 tcttggtctt caaggtaagt tcggttcctt acttctttaa cataatttag gatatcggtt
      121 acattgtttt tgacttcagc agtaatagtg tggtttagtg tatatttttt tggcacctga
      181 tcggctgcgg cctgccgcag cttgcttgtg gcaatggtat ttatcgcttc gtttctggct
      241 aaaatgtttt cctgaagtac ctgaccgact tcaacatcgt atttttgagg taaagcagaa
      301 aaaaacatca tagccgataa gctggcaaag aaaaaaagcc ccattgccag tttctgtaat
      361 actgatttat tgaatgccag cttttgcatt tttatcggaa ttactgtctt tatcatggtc
      421 gcgtaatctc cccgtgttct gttggcgttc tttttcgaat ctttcgtagg ctttaattat
      481 tctctgtacc acctcatgac gcaccacatc cgtgtcatct agatagacaa agtctatgcc
      541 ctttacgcct tttaatactt cctggacctc ttccaacccc gaatatactc ccttaggtag
      601 gtcgatctga gtcaggtctc ctgtaactac cgcctttgaa ccaaagccca aacgggtcaa
      661 aaacatcttc atctgttcag aggttgtatt ctgggcctcg tccaaaatga caaaagaatc
      721 atcaagggtt cttcctcgca tatatgcaag gggcgccact tctataagcc ctttttccat
      781 atagcgccta aaagaatcca ctcctaatat gtcatataaa gcatcatata agggcctaaa
      841 atagggatct accttgtcct gcaagtctcc aggtaaaaag ccaagctttt caccggcttc
      901 tactgccggc ctcgtcaaaa tgattcgatt tacttccttt tccttaaatg ccgttactgc
      961 catggccatc gctaggtagg ttttgcccgt gcctgcaggt cctatggcaa aaacaatatc
     1021 atttcttttt atagcgctaa tatattgctt ctgtcctatg gttttgggtt ttataatctt
     1081 gccccggtag gtatagcaaa ctatgccgtc gtacaaatcg tttatacttt cccctttacc
     1141 gtttttggcc agtgtcacaa aatactttac ttcattaacc gtcaatgagc tgcctctctg
     1201 cagcagttgt atcatttgct tgaaaagttc ttttacagaa tgaacatctt tttcttctcc
     1261 tattatggca atttcaccgc cacgagtcag tatcctcaca ttgaattgtt gttcaataca
     1321 tttaatattt gagtcctgat tcccaaataa aaagcttatg tcatttatat tatctattga
     1381 aactctagcc tccgccaggt tttctgtcaa agtttttcct ccccgtactc aattttttgt
     1441 tttttcccta tatcctctac caattctata atcatcgaac ctattaccgc ttgctcatcg
     1501 gaatccagca catattccat ctttctttga attattttgt tctctttaga gagatgttcc
     1561 aagtccgaaa gtagttgtgc ctccgcttcg atcgaagcat tttcaactcc taaatactct
     1621 ttttgggctt ccatttcaat atactcgact atatttagtt ttaagcctcc gcctaactta
     1681 ggcagtatat taacttcttt taagactctt ttgtcataga gagtataact aatatccccc
     1741 aaatgaaggt ttaaaatgtg attttgaaaa tgcagaataa gggacttttt gctattgccc
     1801 gtggggacct tcttagtctt taccaaggga actttgacgg ccttttgata ccaaaccctt
     1861 gccctaacaa taccctctgc atgaacaaaa agggtgtcgc ttccctcttc ctccccatcg
     1921 ggctcatgac ctagcatgat ttttcccgtt attaggacat ctccttcact taccgtctga
     1981 ccgggctcta ctagcgcctc tcctcttaaa gctattattt cttctatgat gccgtctttc
     2041 gaagcaatta tatcacaggg gatgttttct tcaagttcgg gaggtaattc ctttttcacg
     2101 agttctatta tcagcctgga accccttatc tccatctggg cccaggctaa tcccccatat
     2161 tcgttcagaa gtttatcgat gtagtactcc ttgtcaaggc cgtatttgaa aattccttcc
     2221 ttgatgcccc agctccttaa agcttcatca actgaaatcg ttaattcatc gtccgggcat
     2281 acaatctcta tgctccatat aaaggaagac ataaaatata cagcagctat aaaaagaagg
     2341 gcgccaaaca ggaaccactt tctcttcctt agtttcaata ttacaaagaa caagcctacg
     2401 cgctctatta tatatacgcg gcagcgagtt tttctagcta aagaagcgag tttcgcaaag
     2461 ttttttacgc taactttgca gctgaggaca ttgccgtttt gtcgttttat atcccataag
     2521 tacagatttt ccagggcagc ttgatttaac aacctttcac cgtattctcc tacaattttt
     2581 ataataacat aacctgaaag ataattccat agttttataa aaagcacaag cactgccccc
     2641 ttgcatcatt cctcaaactc aatggcactg attttccctt caaccgttat ttcatcgctg
     2701 attatggttt tgatatggag gtcttcaccc cttacacaaa taacaccaat agatgtgtta
     2761 atcttcacga gctcagaatt gtattcaatg atgcccttgt ggttttctat aaatgtagct
     2821 atcttacccg tcactattac cctgggcaca tttaaaacta tatcctgtgg cagatccagg
     2881 gcgtccgata tctttttctt tatcccctca ctcatacggc taactccttt tgaagcattt
     2941 attaaaattt attcgatttt tgctcgttta attactgtaa aaagaaaaag ccctacagcc
     3001 attgatatgc tctcctttta caaacagaat aatgtccgtt taataaaaga agaacaatca
     3061 aagaccgtag ggtttacttc gataaatgaa ataatatcag tcgtatcgct tcctaatcgc
     3121 tttcggtggt aacagcactt cttttaagat catgcctcga agaatattgt cttgtgtaaa
     3181 aagctcggag aattcaccta ttgatttggc ttgttgcaaa gactcaggtt gcatcctctc
     3241 cgatttaaca gaaatcagct tatttatgtt ttgcacatcc acatcttcga aggggatttc
     3301 caaaatgtca gcttttgttt ctgctttttt tggctccgct atttctatgg ttttaggttt
     3361 tatatcttgt ttgggtggca gttggtaccc tctgggcctt acaggtgttt tgggaaaggg
     3421 gtgcttttgg gttttttgtg tctcgagaag atgactgaaa actgttcgaa agacaaggaa
     3481 gataacaagg agaacaatac tttccatcaa atcacctgct cttatcctgt tttccgggat
     3541 cttcactttc cttttgtccc aacctagata tagagtctct catttgggta tcagctataa
     3601 tgtttttcat gctgtaatag tccataactc ccaatttccc ctgtctcaac gcttccgcca
     3661 tggcttttgg aacttccgct tctgcttcta caactttagc tctcatctcc tgaaccatag
     3721 ccttcatttc ctgctctcta gccacagcca ttgcacgacg ctcttcagcc ttggcctgag
     3781 cgatgcgctt atcggcttca gcctggtcag tttgcagctg agctccgata ttcctgccta
     3841 catccacatc ggcgatatct attgataaaa tttcaaaggc agttcccgca tcaaggccct
     3901 cgcttaaaac agttcgagaa atagagtcag gattttccag aacctcctta tggctctcgg
     3961 atgagccaac ggtagttact ataccttcac caactcgggc aatgatagtt tcttcacctg
     4021 caccgcctac taagcggtca atatttgccc ttaccgtaac tcgggccttt gctttcacct
     4081 caataccatc tttggcaact gccgcaacga taggtgtttc gatgaccttc ggattgacgc
     4141 tcatttgcac tgcttccaat acatttcggc cggccaaatc aatggctgct gccctctcga
     4201 agctcaaggg aatttcagcc ctttgtgcgg caatgagggc atttataact ctgtccacat
     4261 tgccccctgc tagatagtgt gcttctaact tattaacgct cacgtctaat ccggctttta
     4321 ctgcttttat taaggggttt acgattttat ttggaggcac cctacgcagt ctcattccaa
     4381 ccaagttaaa tattcctatt tttacaccgg cagctaaggc cgatatccaa agccccaggg
     4441 gcacaaagct taaaacgact gataaagcga taaaaataac agcgatagtt aaaaataatc
     4501 ctattagtgc ttgcattacg caccctcctc atttttaagt tttactataa ttttgttgcc
     4561 ttcaactctg ctcacaatta tatccgaatt tcttggtata aactcgcctt ctgttaacac
     4621 atcaagcctc ttttcgccga taagagccac tcctgtaggt ctaagagggg tgactgcctt
     4681 gccttcagct cctacaagct ggctttcatc tttaattgat gctacgtagc cttcggactt
     4741 tgcttgtttg gtgcccaaga taattcggtc aaagatcagg gatctgccca aatacttgac
     4801 gaggaaatat atcagcagtg tactaaatac aagggcaacg agaatcgata ttagggcttg
     4861 ttcaaggctg ggaaaggcaa ttattacact tgcaattatg gataaaactc cggtcacgcc
     4921 gaacacacca aaacccggaa taaacatttc gattatcaaa agagccaacc ccaaaataaa
     4981 cagacctatt acccagattt tcgctgctcc cgccaagtaa ctgccaccga aaaataagcc
     5041 aaaggatata aggcctatta taccggggac tccaaaacca ggggttaata tttcagtaat
     5101 tactcccact atgcctaagg tcaaaagaat aggagccaca taggggttgg tgaccatgtg
     5161 ggcaattctc tcagacaggt tcatatcagc tttaacgact tccacattat ttatattttc
     5221 tctttcttta atcttgttta ttagttcttc caaactcgtt acctgttcat cgataagccc
     5281 caattccaac ccttcaccag tagtaagtga taaaatcttt cctttttctt taacaccttc
     5341 tatgacaaca tctgcatcgg ccattgctgc aacaaggtca ggatccctcc ctgtcttttc
     5401 agcaacaccc cgcagggcag aagaccaata ggaaatatat ttttcttcat tcggcctggt
     5461 ttcggcagca ccgatggtac cacctggaac agcaactagt tttttcgaag atatggctat
     5521 tataacacct gcagaagtag catcattgtt tataaagctt acggtaggga taaaggtatc
     5581 gagaatggcg ttgctcattt gtatggcagc gcctacttca ccgcctggtg tatcgatttc
     5641 aaaaattaat gcttttgcac cggctttctc agcttcacct atcattcttg tgacaaatgc
     5701 tgcaagaccc ctgttgatta cgcctttaac aggaatgaca tatatataat cagcagaact
     5761 ttttatgttt tgcccgaagc tatcatttat gaaggcaaaa gtaaataata caataaaggc
     5821 taatattaat actctcttgt ctctcatact actccctccc cacagcaaaa gggttcatat
     5881 ccatcttaac atatttataa gggcttgttt ttataggttt ttatatacat tattaaaaaa
     5941 gcttggcacc caaagatacc aagcttttaa gttactgtaa aagtttctta actaaactgc
     6001 tgacctggct gccatctgca cgtcctttta ctttaggcat caccgcgccc ataaccttgc
     6061 ccatatcctt catgccgttt gcccctactt cggatatagt ctgacgaata atctcttcca
     6121 attcctcctg agtcaatggc tgagggagat agccggacaa gatggcaatt tcttgctcaa
     6181 ggctttttgc aacttcttct ttgccaagtc gcctgtattc ttccatgtct tgctttcttt
     6241 tttttacttc tctaaacaaa actccaatga tttcttcatc attgagttca tggagctttt
     6301 ctttttcagc gtacaaaata gcggactttg ccatgcgtat cacagaaagt ctgtcttttt
     6361 ctcccgcctt taatgccgct ttcatatctt gatttaacat atcttttatt gacatgttat
     6421 cgctccttaa aatttgcgaa atttcctttt tcgagctgcc tcagattttt tcttacgcct
     6481 cacactgggc ttttcataat gctctctttt tcttacttca gaaaggactc ctgctctctg
     6541 acattttctc ttgaatctgc gaagagcact gtccaaggat tcgttttccc ctactatgac
     6601 ttctgacacc tgatattccc tccctccgct gcactttcgg gttaagcttg ccagcacaac
     6661 atgcgaatac aattattata tgcctaaatc tagatatcgt caatatcatc cgggaggcca
     6721 tgtcagaaat ctaccgccta acaggtgaaa gtgcaaatga tttaccgttt gacccccttc
     6781 ttcaccggta tttactacta ttctaaagcc ctttttatct aaattattct ccctggctag
     6841 attttgtgct gtaaggataa tatcgccaag taataagttt tcttcatcat gaatttccat
     6901 gaccgatgtt atgtgttttt tgggaactat aaggagatgg ataggggctt gaggattaat
     6961 atcatgaaag gctacgacat tgtcatcctc atatgcaacc ttagcgggaa tttctttatt
     7021 tatgattttg caaaaaatac agtccataaa aatccacctc cttcatccga tattattgct
     7081 atcccaacta tatcttattc cacatcaggt ggcctatttc ctgctttgtt caaatttttt
     7141 attcatctat ttcgccaaaa atataattgt ccgtgttctt tgaaagcttt actgccttta
     7201 tctgattgtg aagattctct ttggactcca ccatcacttt gatatagttt tccgtaagtc
     7261 cctcatattt gctgtctgct tttttgtctt caaatagcac atatctagta gtatttagaa
     7321 acttttcccg aaaacttcgc tcaagttttt ggctcaattg tataagcctt tgacttcttt
     7381 cattttttat actcttttca atttgatctg gcatgtcggc tgcaaccgtt ccacgccttg
     7441 gagaaaaggg gaatacgtga agctttgaaa actccatctc acttataaag ctttctgtca
     7501 tcttaaattc ctcttcagtt tcgccgggaa atcccactat gacatcggtg gtaatagata
     7561 catcgggcat taattttcta atataattaa ccctattacg aaaatcctca gtgctatatc
     7621 tgcgattcat gcgtttgaga accgtatcgc atccgctttg aagcggtatg tggaagtgat
     7681 gacaaaagct ctcgattttt aataggccat ctaaaaactc atcggtaagc tctaaggctt
     7741 ctatggagct cagccttacc ctctcgatgc cttctatttg ggctatcatc gatatgacat
     7801 ctaacaagtg attcttcttg tctttgaagt caaaaccata taacccaagg tgtattcccg
     7861 tcagcacgat ttctttaaaa ccatcttttg caagacttct tccttctgcc ataatgcttt
     7921 caatggctct gctgcgaatg ggaccacggg catagggaat tatacaatag gagcaaaact
     7981 gattgcagcc ctcctgaatt ttcaatatg
//
LOCUS       DAONUO010000053         7983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6882_length_7983_cov_3.466574, whole genome shotgun sequence.
ACCESSION   DAONUO010000053 DAONUO010000000
VERSION     DAONUO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6882_length_7983_cov_3.466574"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1687)
                     /gene="lysS"
                     /locus_tag="PK768_03035"
     CDS             complement(215..1687)
                     /gene="lysS"
                     /locus_tag="PK768_03035"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQA59979.1"
                     /translation="MDYVNEISEIQKIRLNKLEEIKKMGVAPFGKSFSLTHNLQQIKD
                     NFEELENHDVKIAGRIMAIRGHGKAAFFDIQDQTGRLQMYIKKDGVTEETFQLYKLLD
                     IGDIVGIEGQVFRSKKGEISVAVSDLTLLSKSLRPLPEKWHGLKDTDIRYRQRYLDLI
                     TNPEVKQTFLLRSKIITTMRRYLDERGFIEVETPVLSPIAGGAAARPFITHHNALDID
                     LYLRIATELYLKRLIVGGFEKVYEIGKDFRNEGISIKHNPEFTMMELYQAYADYKDMM
                     DLMEDMVAYIAKEVLGTTKVNYQGEEIDLTPPWKRMTMVEAVKHYTDIDFSQVKSDEE
                     ARDIARKLHIEDVDDDATKGKILNLIFEEKVEANLVQPTFIYDYPIEISPLAKKIEDN
                     PNFTYRFEAFITGREMANAFSELNDPIDQKERFLQQLKEREAGNEEAHAFDEDYIIAL
                     EHGMPPTGGLGVGIDRLVMLLTDSYSIRDVILFPTMRPKQ"
     gene            complement(1705..2181)
                     /gene="greA"
                     /locus_tag="PK768_03040"
     CDS             complement(1705..2181)
                     /gene="greA"
                     /locus_tag="PK768_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQA59980.1"
                     /translation="MARKEVVLTQEGLRKMEEELEYLKSVKRREIAARIKQAIDFGDI
                     SENSEYDEAKNEQAFVEGRIALLEEKLRNVKLIDEVDISTDVVGVGSKIRLKDLDTNE
                     VFEYMIVGSAEADPIKNKISNESPVGKALLGKSKGTVVEIAVPVGTIRYEILDIMK"
     gene            2540..3760
                     /locus_tag="PK768_03045"
     CDS             2540..3760
                     /locus_tag="PK768_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQA59981.1"
                     /translation="MLGLVLEGGGARGAYQIGAWKALRELGMEIKGVAGTSVGALNGA
                     IICQDDFDMAYEIWHNMAYNKILQIDDETISKIKELEVFPENFFHLVKTLRELFREGG
                     LNVSPLRRLLKKFILEDKIRQSGKDFGIVTVDLTDFRPLELYIEDIPSGMLVDYLMAS
                     ANLPFFKIDKIHGKLYIDGGFFDNLPIKLLTAKGYKDIMAVRLYGIGRTRRINKKQLN
                     LITINPSEDLGGTLDFSPERARRNLKLGYYDTLRTIKKYCGNHYCIIANLPEDYFLQM
                     FLKLSPEAVQSLAKILGLPENIPYRRLLLERVIPRISDILGLPRNSSYRDIFIAILER
                     AAISCGIDKFGVYEFDELLELLLDNYHLSSETVSDKIPRIFKQSEIVIKSVKEQLMPE
                     IVDVFVRELKKQAV"
     gene            3849..4760
                     /gene="trxB"
                     /locus_tag="PK768_03050"
     CDS             3849..4760
                     /gene="trxB"
                     /locus_tag="PK768_03050"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQA59982.1"
                     /translation="MVYDVAVIGAGPAGLTAALYAARARLSTVVVEKMFPGGQAALTD
                     IIENYPGFTEGISGIQLADAMKRQAERFGAEFTNGDVERIEKNDDLFTVFLKGESIKA
                     KTVILAAGAQPRKLGVKGEKEFTGRGISYCATCDGAFYKDKPIAVVGGGDTAIQEALY
                     LTRFASEIYVIHRRDQLRATKVLQERAFKNEKIKFVWDSVVKEIKGEGTVDEVVVENV
                     KTGKLDSLRVDGIFIAIGHIPNTDFVKDLVQLNEQGYVITDAYMATNVPGIFAAGDMR
                     QKDLRQVVTAVSDGAIAAVEAGKYLES"
     gene            complement(4908..5591)
                     /locus_tag="PK768_03055"
     CDS             complement(4908..5591)
                     /locus_tag="PK768_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQA59983.1"
                     /translation="MKLIREGKTKTVFETEDGNFLLRFKDDVTGSGDVVDPGANEVIG
                     KIEGKGNASLKLSKYFFAILKSRGIRTHYIDADLEANTMLVKRAETFGDGLEFICRLK
                     AAGSFVRRYGKYVKEGRPLDYLVEITLKDDQAGDPLINKESLVQLGLMAEEEVGEAVN
                     LTKTITKIVEEECRRFGLELLDIKFEFGRVDGEIAVIDEISGDNMRVLKDGKPVFQKE
                     LCEIITGNA"
     gene            complement(5636..6574)
                     /locus_tag="PK768_03060"
     CDS             complement(5636..6574)
                     /locus_tag="PK768_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQA59984.1"
                     /translation="MSETLRVYIGLFGVAFFWGTSWAASKIGLQDFSPLHFTIVRFTV
                     ASLIFYFMLKLFYKDYVIEKRDKRRIWLLGLLGVSIYFLLQHTGLDMTTTVNSSILIA
                     TSPIFTMFLSSKLFRQEQLTYGGIFGALLAFFGVYLVFTGGEGISLGQKTLIGDLLML
                     LNSVVWALFSVLGKDVVDRYDPFVVMAYAYLYGTITMLPFAFTPGFVSSIRAASITTW
                     AAVIYLALFCSVYCYYMWYKGIRVLGASQTAMFNYINPVIAVIIGVIFLKEAWNAYTL
                     IGGILVFLGVYISSAGKGIFARKGSKLPLSESKSQM"
     gene            complement(6858..6983)
                     /locus_tag="PK768_03065"
     CDS             complement(6858..6983)
                     /locus_tag="PK768_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA59985.1"
                     /translation="MINPAITEKDIEELVKTQDFLLENDMLRQAINISDIITDKL"
     gene            complement(6976..7884)
                     /locus_tag="PK768_03070"
     CDS             complement(6976..7884)
                     /locus_tag="PK768_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQA59986.1"
                     /translation="MKNAVKKTLALCLVLFMTLSIVAGCASRQDAAPGNGSTESAEKG
                     SQTPAAAEKSVDEINISYVKLPLNVPSIVEKRLMLFEDEFEKDGIKVNFPEITEGPKM
                     TEALAAGSLDFCNALGGTSAILAAANGVDLKIIGIYSRAPKAFTIMTREDGINSIADL
                     KGKKIAGPKGTILHQLLLEALSRNQMSIDDVEYINMSIPQAVAAMNSDNVDAALVAGP
                     AVPQAMDAGGRIITTGEGLLDATIVIAVRGEFLREHPDLVKRYMNVHYRSLKHMEENP
                     EEVYKMAAEETGLSEENVITMYDWYD"
BASE COUNT         2117 a         1865 c         1694 g         2307 t
ORIGIN      
        1 gctttgtcaa gtcttttttt ataatctctt gaaaagcttt gtcttgctgc gattcgtgct
       61 gtttcccgtg accgctcaaa tataatagca tacttgaacg tgtcatttca aaccttgtta
      121 ttcgtcatta tatgcctttc actattttat gcaccatata ttttcattat gctttaaaat
      181 actacagatt tctacggttt ttataaaaga atttttactg ttttggcctc atggtaggga
      241 ataggatgac atccctaatt gaataggaat ccgtcaggag catgaccaac cggtctatcc
      301 caactcctaa accacccgta ggaggcatgc cgtgttctag tgcaatgatg tagtcttcat
      361 cgaaggcatg ggcctcctcg ttgccggctt cccgctcctt tagctgctgc agaaatctct
      421 ctttctggtc tatgggatca ttcaattcgg agaaggcatt ggccatctcc ctgcccgtaa
      481 taaaggcttc aaatctgtaa gtgaagttgg gattgtcttc gattttcttt gccaatggcg
      541 atatctcaat gggatagtcg tatataaagg tcggttggac taaatttgcc tccacctttt
      601 cttcaaatat aaggtttaat atcttgccct ttgtggcatc atcatccaca tcttcaatat
      661 gtagctttcg ggcgatatcc cttgcctctt catcggactt gacttggctg aaatcaatat
      721 ccgtatagtg ttttacggct tccaccatgg tcatcctctt ccagggtggt gtcaggtcta
      781 tttcttcgcc ctggtagttg acttttgtgg tgcctaagac ttccttggct atatacgcca
      841 ccatgtcctc catcaggtcc atcatatcct tatagtcggc atatgcctga tagagttcca
      901 tcattgtgaa ttcgggattg tgcttaatcg aaatgccttc attcctgaag tccttgccta
      961 tctcgtatac tttttcaaat cctccaacga taagccgctt taaatacagc tcggtggcaa
     1021 tcctgaggta caggtcgata tcaagggcat tgtgatgcgt gataaagggt ctggcggctg
     1081 cgccaccggc tataggtgaa aggacggggg tttcaacttc aataaagccc ctctcgtcca
     1141 aatatcgcct catggttgtg ataattttgc ttcttagtag gaaggtttgc tttacttccg
     1201 gattagtaat caagtccaag tatctctggc ggtatctgat atcggtgtcc tttaagccgt
     1261 gccatttctc cggcagcggc ctcagggact tggagaggag agttaaatcg ctcaccgcta
     1321 cggagatttc tccctttttg gacctaaaaa cctggccctc gatgcctacg atatctccga
     1381 tatccaggag cttgtaaagc tgaaaagttt cctcggtgac accgtccttt tttatataca
     1441 tctgcagcct acccgtctgg tcctggatat caaaaaaagc cgcttttcca tggcccctga
     1501 tcgccatgat tctaccggct attttgacat catgattttc aagttcttca aagttatcct
     1561 ttatttgttg caaattgtgg gtcaaggaaa agctcttgcc aaaaggggcc acacccattt
     1621 ttttgatttc ttcaagtttg ttcagccgaa tcttttgaat ttcactgatc tcatttacat
     1681 aatccatcaa ataacctcca ctgcttattt catgatatca aggatttcat accttatggt
     1741 gccaaccggt acggcaattt ccactacagt gcccttgctc tttccaagca gggcctttcc
     1801 taccggagat tcgttggaaa tcttgttttt tataggatcc gcttcagcag agccaactat
     1861 catatattca aacacttcgt tagtgtcaag gtccttaagt cttattttgg aaccaacgcc
     1921 tacaacatct gttgatatat ccacttcatc gattagtttt acgttcctta gcttttcttc
     1981 aagaagtgca attctgccct ccacaaaggc ctgttcattc ttagcttcgt catattctga
     2041 gttctcgctg atatcgccaa aatcgatggc ctgtttgatt cgtgcagcaa tctcgcgcct
     2101 ttttactgat tttaagtatt ccagctcctc ttccatcttc cttagacctt cctgggttag
     2161 gacaacttct tttctagcca tttaattttc tcccctctgt ttattccaaa attgtatgtc
     2221 cctaatatat gtcatggtag aagcttttat tcaggaaaat aaaacccctt attaaaggga
     2281 caaatgataa taatcctctt catgtaaaat gaagaggcga ggacaaaaaa atccattttc
     2341 ctttgacttc tagattatta taattcagca gtttacttct gtcaagaatc ttcttatatt
     2401 tgttatatta aggcatgcag cgcgtaaaat ttatcataat tgccatacta attgatgcta
     2461 tggtattatt tagatgcaga ttaataagca atttagtttt aaagtagttt atatatcaag
     2521 gggcagtggg ggtattaaaa tgctaggtct cgtgctggaa ggcggaggag ccaggggagc
     2581 ctatcaaatc ggtgcttgga aggcgctgag agagcttggc atggaaatta aaggcgttgc
     2641 cggaacatcg gtgggagccc taaacggtgc cataatatgc caggatgatt tcgatatggc
     2701 atatgaaatc tggcacaaca tggcctacaa taagatactc caaatcgacg atgagacgat
     2761 ttctaaaata aaggagctgg aagtgttccc ggaaaacttt ttccacctgg tcaagacact
     2821 aagggagctt tttagggaag gcggtctcaa cgtaagcccc cttcgccggc ttttgaaaaa
     2881 atttatactt gaagataaaa ttcgacaatc cggcaaggac tttggcatcg tcacagtgga
     2941 cctgactgat tttaggcccc tggagcttta catagaggac ataccgagcg gtatgctggt
     3001 agattacctg atggccagcg caaatttgcc cttttttaaa atcgataaga tacacggcaa
     3061 gctgtacatt gacggcggtt tttttgacaa tctgcccatt aaactcttaa ccgcaaaggg
     3121 ctataaggat ataatggccg tgcgactcta cggtatcggc aggacccgaa ggataaataa
     3181 aaagcagctg aatttaatca ccatcaatcc atcggaggat ctgggaggca ccttggattt
     3241 ttcaccggaa agggcccgga gaaacctcaa gctgggctat tatgacaccc taaggaccat
     3301 aaagaaatac tgcggcaatc attactgtat catcgcaaac ctgcctgaag actacttctt
     3361 gcaaatgttt ttaaaactca gcccggaagc cgtgcaaagc cttgccaaaa tcctcggcct
     3421 tccggaaaat attccttaca ggcggctgct tttggaaagg gttattcctc ggatatcgga
     3481 catattgggc cttccccgaa attcctcgta tagggatatt tttatcgcaa tactcgaaag
     3541 ggccgccata agctgtggga tagataaatt cggagtatat gaattcgatg agttgctgga
     3601 gttgctgctc gacaattacc acttgagttc cgaaactgtt tcagataaaa ttccgcgcat
     3661 tttcaagcaa tcggaaattg taatcaaatc agtaaaagaa cagctaatgc cggagatcgt
     3721 cgatgtattt gtcagggaat taaaaaagca agcagtttga tttaattgtg caaatatatt
     3781 attatatatc aaatatatta ttatatataa tgataatcaa aactaataaa aagaaaggcg
     3841 ggtattcgat ggtctatgat gtagctgtta ttggtgcggg gcctgcaggg cttacagcag
     3901 ctttgtatgc agccagggcc aggctttcca cggtagttgt cgagaaaatg tttccaggcg
     3961 gtcaagctgc tctgacagat attatcgaga attatcctgg ttttaccgaa ggtataagtg
     4021 gcattcagct ggcagatgcc atgaaaaggc aggccgagag attcggcgca gagtttacaa
     4081 acggcgacgt ggagagaata gaaaaaaacg atgacttatt taccgtcttt ttgaaaggtg
     4141 aaagcataaa ggccaaaacc gttatcttgg ctgcaggcgc acagccgcgg aaattgggag
     4201 taaagggtga aaaggaattc acaggccgcg gcatatcata ctgtgccacc tgtgacggcg
     4261 ccttttacaa ggataagccc atagccgtcg ttggcggagg tgatacggca atccaggagg
     4321 ccttgtacct cacaagattc gccagcgaga tatacgtcat tcaccggcgc gaccagctaa
     4381 gggccaccaa agtgctccag gaacgtgctt tcaaaaatga aaaaatcaag tttgtatggg
     4441 attcggtagt caaggaaata aagggcgagg gcactgttga cgaagtcgtg gttgaaaacg
     4501 taaagaccgg caagctcgat tccctgcgag tagacggcat ttttatcgcc atcggccaca
     4561 tacccaatac cgatttcgtc aaggacctgg ttcagctaaa cgaacagggt tacgtgatta
     4621 ccgatgctta catggccacg aatgttccgg gcatatttgc cgctggggac atgcgccaaa
     4681 aggatttgcg gcaggttgtc accgcagttt ccgacggagc catcgccgcc gtcgaagcgg
     4741 gaaaatatct tgaaagctga taaataataa ggcctccatg gtagacagtc ttgttaatta
     4801 gactagctac atggaggcat ttctcttttg cccttgctat gcgatttgcc ttgtccagtc
     4861 tcgtttatgc aaatgcaaaa aactcttggt gtcccgccaa accactgcta tgcattgccc
     4921 gtgattattt cacagagctc cttttgaaaa accggcttgc cgtcttttaa gaccctcatg
     4981 ttgtcccccg agatttcatc tatgacggcg atttcaccat ctacccttcc aaactcaaac
     5041 ttgatgtcta acaactccag gccaaagcgc cggcattcct cttcgacaat cttggtaata
     5101 gtcttggtga gattgacagc ctctcccacc tcttcctcag ccatgaggcc caactgaacg
     5161 aggctttctt tgtttataag ggggtctccc gcctgatcat cttttaaagt tatttcaacc
     5221 aggtaatcca gcggcctgcc ttcctttaca tacttgccgt accttcttac gaagcttccg
     5281 gcagctttca gccggcaaat aaattccagg ccgtcaccaa aggtttcggc ccttttcacc
     5341 agcatggtat ttgcctcgag gtccgcgtca atatagtggg tcctgattcc ccttgacttc
     5401 aaaatggcaa agaagtattt tgatagtttt agggaggcat tgcccttgcc ttcaattttg
     5461 ccgatgacct cgttggcccc cgggtccaca acatcacccg agccggtcac atcatcctta
     5521 aaacgaagca aaaaattacc atcttccgtc tcaaatacag tctttgtttt gccttcccgt
     5581 attagtttca agttcgcatc ccccttattt atataagttt cacacaaatg aaattctaca
     5641 tttgtgattt gctttcggat aatggaagct ttgacccttt tcttgcaaat attcccttac
     5701 cggcggaact tatatacaca cctaagaaga ccaatatgcc acctattaaa gtatacgcat
     5761 tccaggcctc ttttaaaaat atcacgccga taattaccgc tatcaccggg tttatataat
     5821 tgaacatggc cgtctgggag gctccgagca cccttatgcc cttgtaccac atgtagtaac
     5881 agtaaaccga gcaaaacagg gccaggtaga ttacagcggc ccaggtagta atcgaagccg
     5941 cccttataga gctgacaaat ccgggtgtaa aggcaaaggg cagcatggtg atcgtgccgt
     6001 acagataggc ataggccatc accacgaagg ggtcgtacct gtcaactacg tccttgccca
     6061 aaaccgaaaa tagggcccaa acgaccgaat ttaagagcat gagtaaatca cctatcaagg
     6121 tcttttgccc aagggaaata ccctccccgc cggtgaaaac taaataaact ccaaagaagg
     6181 ccagcagtgc accgaaaatg ccgccgtatg tcagctgttc ctgccggaat aattttgaag
     6241 atagaaacat tgtaaaaatc gggcttgtgg ctattaatat tgatgaattg acggtggtgg
     6301 tcatatccag ccccgtatgc tgtaggagaa aataaattga tacccccagc aatcccaaga
     6361 gccaaattcg ccttttatcc cgtttttcta taacatagtc tttgtaaaaa agctttagca
     6421 taaaataaaa aatcaaagaa gcaactgtaa acctcacgat tgtaaaatgc aacggcgaaa
     6481 aatcctgaag gccaattttt gaagcggccc aggaggttcc ccaaaaaaag gcaactccaa
     6541 aaaggccgat atatacgcgt agagtttcag acatgctagc acctcgtaat atccttgcaa
     6601 gattaacaat ataataacac attttgcgct gcggcggtat agcaaatgta tcgcaaatta
     6661 ctcaagaaat gaaggtaacg gcagtctttt aagtgggcaa tttccaatga ggcaaggtaa
     6721 cggtgcccca taaaaaataa agccttggag aaagcttatc attgcaaagc tttgctagtc
     6781 caaggccaag ttgccgctga caagtcaggc tgtaagataa gctgcgttat ctttgctgtt
     6841 gaagcttcga tccctgttta cagtttgtca gttataatat ccgatatgtt tatcgcttgt
     6901 cttagcatat cattttccag caggaagtcc tgggtcttta caagttcctc tatgtccttt
     6961 tccgttattg cagggttaat cataccagtc gtacatggta atcacgtttt cctcggaaag
     7021 tcccgtctcc tctgccgcca tcttgtaaac ttcttccggg ttttcttcca tgtgcttcaa
     7081 actcctgtag tgtacattca tgtacctctt taccaggtcg ggatgctctc ttaaaaactc
     7141 tcccctaacg gcaataacta tggttgcatc cagcagtcct tcccctgtcg ttattatgcg
     7201 gccacctgca tccattgcct ggggcactgc aggacctgcc acaagggcag catccacatt
     7261 atcggaattc atcgctgcca ccgcctgggg aatgctcatg ttgatgtact ccacatcatc
     7321 gatgctcatt tggtttcggc taagggcctc aaggagaagc tggtgcaaga tggtgccttt
     7381 tggacctgca atctttttgc ccttcaggtc tgctatggag tttatcccgt cttccctggt
     7441 cattatggta aaggccttag gagccctgct gtaaatgcca attattttca ggtctactcc
     7501 gttggcagca gcgagaattg cagaggtgcc ccccagggca ttgcaaaaat ctagggaacc
     7561 tgcggctagg gcttcggtca tcttgggccc ttcggttatc tcggggaagt tgacctttat
     7621 gccgtctttt tcaaactcat cttcaaagag catgagtctc ttctcaacga tagaggggac
     7681 attcagtggc agctttacat aggaaatatt tatctcatcc acgctttttt ctgcggctgc
     7741 aggagtctgg ctgccttttt ctgctgattc agtactgcca ttaccaggag cagcatcttg
     7801 tcttgatgca caccccgcga ctatcgacag ggtcataaaa agcaccaggc acaaagccaa
     7861 tgttttttta acagcatttt tcattatacc tacatctcct ccgctaaatt ttacattgta
     7921 tatatatcta gtcgccctgc tctcccatga tagcccctag tatttcctgg cggtacttaa
     7981 aaa
//
LOCUS       DAONUO010000054         7966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6902_length_7966_cov_3.772848, whole genome shotgun sequence.
ACCESSION   DAONUO010000054 DAONUO010000000
VERSION     DAONUO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6902_length_7966_cov_3.772848"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..73)
                     /locus_tag="PK768_03075"
     CDS             complement(<1..73)
                     /locus_tag="PK768_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HQA59987.1"
                     /translation="MFKFSDEKSQLKCSFCGKTQDQVR"
     gene            complement(94..678)
                     /gene="clpP"
                     /locus_tag="PK768_03080"
     CDS             complement(94..678)
                     /gene="clpP"
                     /locus_tag="PK768_03080"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQA59988.1"
                     /translation="MSLVPIVVEQTNRGERAYDIYSRLLKERIIFIGTAIDDTVASLA
                     IAQMLYLESEDPDKDIRLYINSPGGSVTAGLAIYDTMQYIKPDVSTICMGLAASMGAV
                     LLAAGTEGKRYALPNSRIMIHQPWGGVQGKAVDIETHAKEILRLRELLNEILSKHTKQ
                     PIEKIDKDTDRDYFMSSDEALKYGLIDEVIHKRK"
     gene            complement(707..2074)
                     /gene="tig"
                     /locus_tag="PK768_03085"
     CDS             complement(707..2074)
                     /gene="tig"
                     /locus_tag="PK768_03085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQA59989.1"
                     /translation="MKVNLEKIEKNKAYLNFEIDAQQFDEAMERAYRKNARRFVIPGF
                     RRGRAPRKLIERHYGPEVFFEDAARFLLPEAYQKGVEEYKLEPVDQPTFDIKQIEVGK
                     PFIATAEVFVKPEVDIKEYKGLEVEKIVYNVTDEDVEKELSQLQEQNARLVTVEDRAA
                     KMEDIAVIDFKGTIDDEPFEGGTAENFSLKLGSGMFIEGFEEQVVGMNVGESKDIEVT
                     FPEDYSAKELAGKKAVFNVTLKELKAKELLPLDDDFAKDVSEFETLDELKQDIKTKLE
                     EQAKSVEEGSLKASIVNKLKEVAEVEIPDVMIDQEIDRLVFDFAISLRYRGYDLQSYL
                     DATGLSLEKFKESFRERALDNVKSSLILEEVGKRENITVTDEELENKIKELAESLKQS
                     VEDYKKNLKEEDMARIKDSILTNKIFDFLISHSNIVEKSKDDVKDDIIESEEDSKADS
                     EENEN"
     gene            complement(2212..2297)
                     /locus_tag="PK768_03090"
     tRNA            complement(2212..2297)
                     /locus_tag="PK768_03090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2261..2263),aa:Leu,seq:tag)
     gene            complement(2299..2374)
                     /locus_tag="PK768_03095"
     tRNA            complement(2299..2374)
                     /locus_tag="PK768_03095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2339..2341),aa:Lys,seq:ttt)
     gene            complement(2378..2451)
                     /locus_tag="PK768_03100"
     tRNA            complement(2378..2451)
                     /locus_tag="PK768_03100"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2417..2419),aa:Gln,seq:ttg)
     gene            complement(2522..2598)
                     /locus_tag="PK768_03105"
     tRNA            complement(2522..2598)
                     /locus_tag="PK768_03105"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2562..2564),aa:His,seq:gtg)
     gene            complement(2605..2681)
                     /locus_tag="PK768_03110"
     tRNA            complement(2605..2681)
                     /locus_tag="PK768_03110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2645..2647),aa:Arg,seq:tct)
     gene            complement(2683..2759)
                     /locus_tag="PK768_03115"
     tRNA            complement(2683..2759)
                     /locus_tag="PK768_03115"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2723..2725),aa:Gly,seq:tcc)
     gene            complement(2761..2837)
                     /locus_tag="PK768_03120"
     tRNA            complement(2761..2837)
                     /locus_tag="PK768_03120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2801..2803),aa:Pro,seq:tgg)
     gene            complement(2955..3425)
                     /locus_tag="PK768_03125"
     CDS             complement(2955..3425)
                     /locus_tag="PK768_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA59990.1"
                     /translation="MKIGVLSDTHGVRFIAKKAVNKMEDIEILLHAGDLVEDARHLEG
                     NNYRVYCVAGNCDAFSLEPTEKILELQGKKILLTHGHTYRVKRGYEQLLFRAKEVSAD
                     IVVFGHTHVPENTYIDNILFFNPGSTALPKNGGPGTYGIIEIADGRIKACINSI"
     gene            complement(3436..4026)
                     /locus_tag="PK768_03130"
     CDS             complement(3436..4026)
                     /locus_tag="PK768_03130"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HQA59991.1"
                     /translation="MNTLVIATKNHGKFLEFKEMLGPASFSIVSLIDFHHIEIQETGS
                     TFDENALIKATTVCRETGLPTLADDSGLEVHILNGEPGVLTARYAGEHATDEDNINKL
                     LERLEGVPLDKRRAQFVCSLALVFPDGQAFFERGILEGLIALSPRGTEGFGYDPIFLL
                     PEQNKTLAEISIGEKNRISHRARALEKIKKHLFRLI"
     gene            complement(4042..4767)
                     /gene="rph"
                     /locus_tag="PK768_03135"
     CDS             complement(4042..4767)
                     /gene="rph"
                     /locus_tag="PK768_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQA59992.1"
                     /translation="MDILARVDGRGNDEIRPITITRNFNKYAEGSVLIEAGDTKVTCT
                     ATVDDKVPPFLRGKGQGWITAEYSMLPRATEARNVREMLRPSGRTMEIQRLIGRALRS
                     VVDLSALGERTIWLDCDVIQADGGTRTLSITGAFIALADAIKFLKENNLIETNPINNF
                     VAAISAGIVDGERLLDLVFEEDCRAQVDMNVVMTDTGQIVEIQGTGEAFPFSREELDG
                     LMDLAFKGISELIQIQKKLIIDI"
     gene            complement(4801..5805)
                     /locus_tag="PK768_03140"
     CDS             complement(4801..5805)
                     /locus_tag="PK768_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HQA59993.1"
                     /translation="MVKKIVCLLIILFLVAAAVAGCDFFKRESPQEEVPDSGEDSEET
                     LANMRKTIFYFVNEYGLLVPVTRDIPWVEGIGRAALESLVDSEEMRAELAEKGLKAPL
                     PEGTQVLGMTIRDGLAKVDFNANFLQTPDIVTEQNAIDAVVYTLTEFPAVDEVQILVD
                     GKMVEKTPNGNQLKGVFKRQNINLEKPVTASSEMVPVTLYFKGASLNGNFSYFVPVTR
                     MVTKSDNQMKTALEELIKGPMENTGLVSLLPQDTKVLDVAQSNGEVLVNFSKEIEGYG
                     GGIDVEQSIVNSVVLTLSDFPGVETVTIQVEGKAGALPEGTVLETPIYKPLYVNPSSI
                     "
     gene            complement(5878..6399)
                     /locus_tag="PK768_03145"
     CDS             complement(5878..6399)
                     /locus_tag="PK768_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HQA59994.1"
                     /translation="MWSLIGGYLFIFLARVVDMSLTTMRTLMLVRGKGLIAASIGFFE
                     VIIYITALNKVVSGLSNPVNLLAYALGFATGNYMGTLIEEKLAIGLTTVQIISHKNGI
                     GDKIRDMGFGVTSLEGKGREGIKEVLIVSLPRKELDNLLAFIDKNDSHAFITIMDTRA
                     SKGGYFKQTVKSK"
     gene            complement(6470..>7966)
                     /locus_tag="PK768_03150"
     CDS             complement(6470..>7966)
                     /locus_tag="PK768_03150"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQA59995.1"
                     /translation="GSKVVDLAYETIDGKPTVIIKGDSLLEYTTVEIEDDNKLVIDIK
                     SHLDTLKNALYAYDNYLSKAVAGEIFTDPPTTRVVLELGPRVSYRTYSSADKKSIFIS
                     FENTVEELSFESQRHELLARIKTTNVTTGDYFFLSNPDRLVLDISETLLAEIDIPEVP
                     VNDFVKAVRLGQFTDDMVRVVFDLKNDINYQVFQEDNVFSIIFSQVRTVEDIDIVSER
                     NISFVEISASDEIGYELKADKNKKQIKIVMPGVAIGDRLVSRDVIRVSDGIIDYIELV
                     KVKAAKNYNLEIVINLHSFTSYEMLSSPPSSNIKLALHSSPLINKLVVIDPGHGGSDP
                     GAVVGNVNEKDLNLDVALRLKYLLEANGVRVVMTREDDTFVNLYARAGIANEVDADLF
                     ISIHHNTATSSASGTETLYYPDPEKKLFAQALQDAVVRQTGFPSRGIVERPGIVVTRE
                     TKMPSALVEVGFMSNKNELAIMMTDEFKNQVAQGLLQGIVDYLSGMTN"
BASE COUNT         2027 a         1965 c         1473 g         2501 t
ORIGIN      
        1 tacgtacctg atcctgagtc ttgccgcaga acgagcattt cagttgactc ttttcatcgc
       61 taaatttgaa catctttcac tctcctttcc ggcttacttc ctcttgtgga tcacctcatc
      121 aatcaagccg tactttaaag cttcatctga tgacataaag taatccctgt ccgtatcctt
      181 gtctatcttt tctatgggtt gcttggtgtg ctttgaaagt atttcattta gcagctctct
      241 taggcgaaga atttctttcg cgtgtgtttc tatgtctaca gctttaccct ggacaccgcc
      301 ccaaggctgg tgaatcatga tgcgtgaatt gggcagtgca tagcgcttac cttcagttcc
      361 ggctgccaaa aggacagctc ccatgcttgc agcaagcccc atacaaatag ttgaaacatc
      421 gggcttgatg tattgcatcg tgtcgtaaat ggcaaggcct gctgtaacgg agccccccgg
      481 actattgata tagagccgga tgtccttgtc aggatcttct gactccaggt acaacatttg
      541 agctatagca aggcttgcga cagtatcgtc tatggctgtt ccaatgaaga tgattctttc
      601 tttgagtagt cgggaataaa tatcgtatgc tctttcccca cggttagttt gttccacaac
      661 tataggaact aaactcacta taaatacctc cttaaattac accattttaa ttttcatttt
      721 cttcactgtc ggctttgctg tcttcctctg attctattat atcatctttt acgtcatctt
      781 tgctcttttc tactatattg gaatggctga ttagaaaatc gaagatttta tttgtaagta
      841 ttgagtcttt tattcttgcc atatcttctt cttttaaatt ctttttataa tcttccaccg
      901 actgttttaa gctttcagct aattccttga ttttgttttc aagctcctca tctgtcacag
      961 taatgttttc ccttttgcct acttcctcta atatcagtga tgattttaca ttgtcaagag
     1021 ctctctcgcg gaaactctct ttgaattttt caagactaag gcctgttgca tccaaatagg
     1081 actgcaaatc atatccgcga tatctcaggc ttatagcaaa atcgaagact aacctatcaa
     1141 tttcctgatc tatcattaca tcaggaattt cgacttcggc aacttccttt aatttattta
     1201 ctatagatgc ttttaaagag ccttcttcca cggatttggc ttgttcctct aattttgtct
     1261 tgatgtcctg tttaagctca tccagagttt caaattcgct gacatccttg gcaaaatcgt
     1321 catccagagg cagcagttct tttgccttaa gttcctttaa agtcacgtta aaaacagcct
     1381 tttttcctgc caactctttt gcgctatagt cttccggaaa agttacttcg atgtccttgc
     1441 tttctccgac attcattcca acaacttgtt cttcgaagcc ctctataaac atacctgaac
     1501 ccaatttcag agaaaagttc tctgcagtgc ctccttcgaa gggttcatcg tcaatcgtac
     1561 ccttaaagtc tatgacagct atatcttcca ttttggccgc cctgtcttca accgttacga
     1621 gtctggcatt ttgctcctgc agctgagaca gctctttttc tacatcttca tcagttacat
     1681 tatagacaat tttttctact tctaatcctt tatattcctt tatatcaact tcgggtttga
     1741 caaatacttc tgcggtagct ataaagggtt tgccaacttc tatctgtttg atgtcaaaag
     1801 ttggttgatc tacaggctcc aacttgtatt cttctactcc cttttgatag gcttcaggca
     1861 aaagaaatct tgcagcgtct tcgaaaaaga cttcaggacc gtaatgtctt tcaatgagct
     1921 ttctgggagc tcttccccgc ctaaatccgg ggattacaaa gcgccttgcg ttttttctat
     1981 acgcacgctc cattgcctca tcgaattgct gtgcatcaat ctcgaaattt aagtaggcct
     2041 tgttcttttc tattttttcc aggtttactt tcattatgct cctcccttgc aattcccgta
     2101 aattccattt cttttgatat tataacataa aaaaatatat gcaaacaaaa aattacaata
     2161 aaataacacc tcggtatgga ccgaggtaga aaaatccaca gtatttgaat atggtgcggg
     2221 caagaggatt tgaacctcca cgggataaat ccaccagatc ctaagtctgg cgcgtctgcc
     2281 agttccgcca tgcccgcatg gtgggtcatg gaggactcga acctccgact ccctgattaa
     2341 aagtcaggtg ctctaccacc tgagctaatg acccttttgg ctggggcggc aggattcgaa
     2401 cctacgatgc aggagccaaa gtcctgtgcc ttaccgcttg gcgacgcccc aataaattta
     2461 cagtagccca aaaagggttg tcggaattct atttgtagca tccgacatta aatttgtatt
     2521 atggggtgga tagtgggact tgaacccacg acctccagag ccacaatctg gcgctctaac
     2581 cacctgagct atacccacca tatatggcgc gcccgaaagg attcgaacct ttggcctacg
     2641 gattagaagt ccgttgctct atccagctga gctacgggcg cctggagcgg gaaacgggat
     2701 tcgaacccgc gacattcagc ttggaaggct gacgctctag ccagctgagc tattcccgca
     2761 tggtcggggc agagggactt gaacccccga ccccatggtc ccaaaccatg tgcgctacca
     2821 aactgcgcca tgccccgaca tcttaaagac attgtatagt atacatgaaa tagttttttg
     2881 agtcaagatt attttagcac aacttttgtg gtatttcaaa tcgattttta tttttcgatg
     2941 cccgaaaacc ctgcttatat actgtttatg catgccttga tacggccatc agcaatttct
     3001 atgattccat atgtcccggg cccaccattt ttcggcagag ctgtgcttcc gggattaaaa
     3061 aacagtatgt tgtcaatata tgtgttttcc ggaacatggg tgtggccaaa taccactatg
     3121 tctgcagaaa cttcttttgc cctgaaaagt aattgttcgt agccccgctt aactctgtat
     3181 gtatgcccat gggttaatag gattttcttg ccctgaagct ccagtatttt ttcggtcggt
     3241 tcgaggctaa aagcgtcgca attccccgct acgcagtaga cccggtaatt attcccttcg
     3301 agatggcggg cgtcttcaac caaatcgccc gcgtgaagga gaatttcaat atcttccatc
     3361 ttattcacgg cttttttcgc gatgaatctt acgccgtgag tatcgcttaa cactccgatc
     3421 ttcaagatat tcctcctata taagcctaaa taaatgtttc ttgatttttt ccagagccct
     3481 tgccctatga cttatcctgt tcttctctcc tatggaaatc tcggcgaggg ttttgttctg
     3541 ttcaggcaac agaaatatcg ggtcatatcc aaagccttcg gtccccctag ggctaagagc
     3601 gataaggcct tctaggatac cccgctcgaa gaaggcttgt ccatcaggaa agacaagggc
     3661 caggctacaa acaaattgag cccgcctttt gtctaatgga actccttcta atctttcaag
     3721 gagtttgttt atattgtctt catcagtagc atgttctccc gcatacctgg cagttaagac
     3781 acctggttca ccgtttagga tgtgtacttc aaggcccgaa tcatcagcca gggtcggcaa
     3841 gccagtttct cggcatactg ttgttgcctt tatcaaggca ttttcatcaa aagtagagcc
     3901 ggtctcctga atctcgatgt gatgaaaatc tatcagcgac acaatactaa agcttgcggg
     3961 ccccaacatt tccttaaact ccaagaattt gccatgattt tttgttgcaa taaccaatgt
     4021 attcatgttt aaaagctccg cttaaatatc tatgattaac tttttttgaa tttgaatgag
     4081 ttctgatatg cctttgaagg ctaaatccat caacccatcc aattcctccc tgctaaaggg
     4141 aaaagcctct ccagtacctt gaatctctac tatttgtccc gtatcggtca taaccacatt
     4201 catgtctacc tgagctcgac aatcctcttc gaatactaaa tccagtaatc tctcgccatc
     4261 cactatacca gcacttatag ccgccacgaa attattaatg ggatttgtct ctatcaaatt
     4321 gttttctttc aaaaacttaa ttgcatccgc taaggctata aaggcgccgg ttattgatag
     4381 ggtcctcgtt ccaccgtctg cttgaataac gtcgcagtca agccagatgg tcctttcgcc
     4441 taaggctgaa aggtcaacta cagaccttaa agccctgcct attaatcgct ggatctccat
     4501 ggtcctgccg ctgggcctta gcatttcccg cacgtttctt gcttccgtgg ccctgggcag
     4561 catggaatac tcggcggtta tccagccctg gcccttgcca cgcaaaaatg gtggcacctt
     4621 atcgtccact gttgccgtgc aggtaacttt cgtatctcca gcctcaatga gcacggaccc
     4681 ttccgcatac ttattaaagt ttctggttat ggttataggc cgaatttcgt cattgcctct
     4741 tccgtctact cttgccaaaa tatccatcct ttctcacaag ttgatatcta tacaaaaatt
     4801 tcaaatgctg ctcgggttga catagagggg tttatatata ggtgtttcaa ggacagtgcc
     4861 ttccggtaaa gctcctgctt taccttccac ctggatggta accgtctcca ctcccggaaa
     4921 atctgaaagg gtgagaacca ccgagtttac gatggattgt tctacatcta taccgccacc
     4981 gtatccttca atttcctttg aaaaattaac cagcacctct ccattgcttt gagctacatc
     5041 aagcaccttc gtgtcttggg gcaacaatga tacaaggccg gtattttcca tgggaccttt
     5101 tatcaattct tccagggcgg ttttcatctg attgtcactt tttgtcacca ttcgagttac
     5161 cggaacaaag taactgaagt ttccatttaa gcttgcacct ttaaaatata aagttaccgg
     5221 taccatttcc gatgaagctg ttaccggttt ttctaagttt atattctgcc gtttaaaaac
     5281 accctttagc tgattgccgt ttggtgtctt ttccaccatc ttcccgtcaa ctaagatttg
     5341 aacttcatca accgcgggaa attcagtcaa ggtatatacg actgcgtcga tggcattttg
     5401 ttcagtcact atatccggag tctgcaagaa atttgcatta aaatcaactt tggccagacc
     5461 atcccgtatt gtcattccta gaacctgtgt accttccggc aagggagctt ttaagccctt
     5521 ttccgcaagt tctgctctca tctcttcact gtcaaccagg ctctccaacg ctgcccttcc
     5581 tataccctca acccatggaa tatcccttgt tacaggcact aacaagccgt attcatttac
     5641 aaaataaaag atcgtctttc tcatatttgc cagggtttct tcagaatcct cgccggagtc
     5701 cggaacttct tcctggggac tttctctttt aaagaaatca cagcctgcaa cagctgccgc
     5761 cactaaaaac aatatgatta acaagcaaac aatcttcttg accatgcata aaaacctcct
     5821 ctttttaatc taattaatat atattaacga gaagaggttt tttatgccgt caaagtatta
     5881 tttgcttttt acggtctgtt taaagtatcc ccctttgcta gccctggtat ccattatcgt
     5941 aataaatgca tggctatcat ttttatcaat gaaggccaaa agattatcaa gctcttttct
     6001 gggcaaggaa acgatcaaga cttccttaat accttccctg ccctttcctt caagacttgt
     6061 tacgccaaaa cccatgtcgc gaatcttgtc acctatgccg tttttgtgag aaattatctg
     6121 aacggtggtt aaacctatgg caagcttttc ttcaatcaaa gttcccatat agtttccggt
     6181 ggcaaagccc agggcgtagg ccaatagatt caccgggttg ctcaagccgc tgacaacttt
     6241 gtttaaggcc gttatgtaaa tgatgacctc aaaaaaacca atacttgcag caatcagccc
     6301 cttgccccta acaagcatca gggtccgcat ggtagttaaa gacatgtcta ctactctggc
     6361 aaggaagata aatagatatc ccccgataag cgaccacaaa ccaaattcct cctacggtaa
     6421 atttaatttc cttaatttaa cgtaatgata tcaaaacggc tatcgtgtgt caattggtca
     6481 ttccgctgag ataatcgacg atgccttgga gcaatccttg ggcaacctgg tttttgaatt
     6541 catccgtcat cattatcgca agctcatttt tattcgacat gaaacctact tctaccaatg
     6601 ctgaaggcat tttcgtctcc cgcgtaacga cgatgccggg tcgttcaaca atgcccctgc
     6661 tgggaaaacc ggtttgcctg acgacggcat cctgcaatgc ttgcgcaaat aattttttct
     6721 caggatccgg gtaatacaaa gtctcagttc ccgatgcgct gcttgttgca gtattgtggt
     6781 ggatactgat aaacaaatct gcatcgactt catttgctat gcctgcacgg gcatatagat
     6841 tcacaaaagt atcatcttcc cgcgtcatga ctactcttac accatttgcc tctaaaagat
     6901 actttaatct caacgcgaca tcaaggttga ggtctttttc atttacattc ccgactacag
     6961 ctcccgggtc gctgccacca tggccaggat cgataactac cagtttattg atcaagggag
     7021 aactgtgcag cgcaagtttt atattagagg aaggaggcga tgacagcatt tcataacttg
     7081 tgaaagaatg caggtttatg actatttcca agttgtaatt tttagcagct tttaccttca
     7141 ccagttcaat atagtcaata ataccatctg atactcttat cacatcgcgg cttaccagcc
     7201 tgtccccgat ggctacgccg ggcataacga tttttatttg ctttttattc ttgtccgcct
     7261 taagctcgta tcctatttca tcactggccg aaatttccac gaagctaata ttcctttcac
     7321 tgacaatatc gatgtcttct accgtgcgta cctgtgaaaa gatgatcgaa aaaacgttgt
     7381 cctcctggaa aacttgatag ttgatgtcat tttttaaatc aaataccacc ctcaccatat
     7441 cgtcagtaaa ttggcccaag cggacagcct tcacaaaatc atttacagga acttccggaa
     7501 tgtcaatttc agcaagtagg gtttcactga tgtccaagac cagtcggtcc ggatttgaca
     7561 aaaagaaata gtcgcctgtc gtaacatttg tcgtcttaat ccttgcaagc aattcatgcc
     7621 tctggctttc aaagctaagc tcttctactg tgttctcaaa ggatatgaaa atacttttct
     7681 tatcggctga actataggtc ctataagata ctcttggtcc tagttccaac actacccgtg
     7741 tcgtaggtgg atccgtaaaa atctcacctg ctacagcctt gcttaaataa ttatcgtatg
     7801 cgtacagggc attcttcaag gtatctaagt ggcttttgat atcgattacc agcttattat
     7861 catcttctat ctcaactgtt gtatattcta aaagactgtc cccttttata ataaccgtgg
     7921 gcttaccatc aatcgtttca tatgcaaggt ctacgacctt ggaccc
//
LOCUS       DAONUO010000055         7916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6958_length_7916_cov_3.888691, whole genome shotgun sequence.
ACCESSION   DAONUO010000055 DAONUO010000000
VERSION     DAONUO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6958_length_7916_cov_3.888691"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PK768_03155"
     CDS             <1..822
                     /locus_tag="PK768_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA59996.1"
                     /translation="PGKEIVILGSGDIGLIMARRFTLEGAKVKCVAEIMPYSNGLTRN
                     VVQCLEDFGIPLLLSHTVVYIHGNSRIEGVTIAQVDENLKPIPSTEKYYSCDTLLLSV
                     GLIPENELSKMASIKIHDVTGGPVVNQRMETSVPGIFACGNVVHVHDLVDFVTMESRL
                     AGLGAVDYLKDKIPSDEYISVSAGPGISYVVPHLVDPEKILGEKQNFFMRSTKPMEKA
                     RLFIESDKNLIRTRVLQHVRPSEMISIELKKEDLANKDLKSLRFFLQEEGVADGN"
     gene            812..1165
                     /locus_tag="PK768_03160"
     CDS             812..1165
                     /locus_tag="PK768_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HQA59997.1"
                     /translation="METKKITCIVCPKGCQIQVKLNNGEIVSIEGYECKRGIDYAKNE
                     VLDPRRTLTTTLKLSTGGVLPVRTKEPIPKKLLKKAMLETKDIIVEPPVKVGDVVVEN
                     IAGTGIDLIATGNVE"
     gene            1200..2879
                     /locus_tag="PK768_03165"
     CDS             1200..2879
                     /locus_tag="PK768_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQA59998.1"
                     /translation="MLQKDAVFTHPKGMHIRAAAMLVNKAHEIESTMGTSIYIRNESG
                     REVPATALMAIHSLNIKPGERITIVAKGPKVDEAIESVEELFRRDLLPQSPIELEQVD
                     DILEKNTIKADKIFDSVGLGIIVTNKTGAVILCNRIVEELMGIDKNHILGCNNIQDII
                     PGIDLTTTSSIGSGIFRLDDNTRSNLASTDINPIFIDGEIAGYTIVFNKDVSTAESFS
                     PEYTLEISPTKDLLIEQKKRMEFNEAFSDIIGRSEKFAVVLDIAFKAAKTSSTILIQG
                     ESGTGKELVAKAIHRASSRRNGPFIKVNCPGIPATLMESELFGHERGAFTGAIFQKIG
                     KFELANGGTIFLDEIGELDKNLQSKLLRVLQEREFERVGGNEVLKTDVRVVAATNRNL
                     KEMVERDEFREDLYYRLNVVPIVLPSLRERKDDIPLLVEHFLEKLCRKLGKKEKTITK
                     RAMGYLYNYDWPGNVRELENIIERMINLSDSDVIDIWDLPSHVTGKAMEKKASLINLD
                     LDGELATYDEYDKAIIKTALKKYKSFNAAGKALGLTHKTVAAKARKYGLVK"
     gene            2903..4639
                     /gene="phnD"
                     /locus_tag="PK768_03170"
     CDS             2903..4639
                     /gene="phnD"
                     /locus_tag="PK768_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HQA59999.1"
                     /translation="MANSGKSIDKVSPASQSEILRLSEELGYDAHEIKWVVAQNTETI
                     RNLVKVIETISSHSQEISANTASGSEQLNNLSRLAENLRETASSVASISRDYLSKVKE
                     GSLAISDVETALEQVTAEIDKSSDEVETLLELTKQVKGFVDFIKHIAQQTNLLALNAA
                     IEAARAGEAGRGFSVVAAEIRKLAERSKDKAQEIHKTAELIDDGISRACRISKDGAVS
                     LKNLKSKMQLGKDVMEGAVAAFEDISELNSNLLDSSANQAKSTQYLADIFASFSEKTA
                     STADSTIEVAYLIKDQEKQNEQLLNIADNLVDKVYSLQKQSTSLKKKEEIIFGINPAL
                     SPEIIKSLYLPVINTVCKKVGLIPRVLVATDYDALANSLIDGIVDVGWFSPLAYVNAK
                     NKAAVIPLVTPIVNGAPDYLGYIITNRESGITDLKDVRGERMAFVDPKSASGYAYPRL
                     ILKKAGIDPERDLKEQLFLGTHSNVVQAVISGSVRVGATYSEAIDDAKSRGLEVDKLV
                     YLAQTDPIPKDCIAARPGFDRELCEKLKNAFLKYEDIGLLDKHKSSVINGFVIASDEN
                     YDIIREVLRDAG"
     gene            4760..5758
                     /gene="dhaK"
                     /locus_tag="PK768_03175"
     CDS             4760..5758
                     /gene="dhaK"
                     /locus_tag="PK768_03175"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HQA60000.1"
                     /translation="MKKIINSPEEVVQEMLEGMAAAYPQYVRKLEGFEVMVRADAPVK
                     GKVALVSGGGSGHEPSHAGFVGKGMLDAGVAGAVFTSPTPDQVFEAIKAVDGGAGVLL
                     IIKNYTGDIMNFEMAGEMAEAEGIKVASVVVNDDVAVEDSLYTTGRRGIAGTVFVHKI
                     AGAKAEAGGTLEEVKAVAEKVIANTRSMGMALTPCIVPAAGKPTFTLGEDEMEIGMGI
                     HGEPGTKRTHIMKADELVDHLMEKIVNDLPYESGDEVAVMINGLGATPLMEQFIMNRR
                     VSQILNEKGIKVYRTFVGEFMTSIEMAGASITLLKLDDELKELLDAQADTPAFRQL"
     gene            5785..6411
                     /gene="dhaL"
                     /locus_tag="PK768_03180"
     CDS             5785..6411
                     /gene="dhaL"
                     /locus_tag="PK768_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HQA60001.1"
                     /translation="MAVNSAKLIEILNLIANDIHANKDFLTELDSAIGDADHGINMSK
                     GFKAVTEKIAAMGDKDCGSILKTVGMTLVSTVGGASGPLYGTAFMRAGQAIGSKTELG
                     FEDLLVTLEAALEGIKMRGKAVKGEKTIVDALEPAVEALKSSPTININLMEEAVKAAE
                     AGVEYTKGIIAKKGRASYLGERSIGHQDPGATSCHIMLTAILKGIKDS"
     gene            6438..6833
                     /gene="dhaM"
                     /locus_tag="PK768_03185"
     CDS             6438..6833
                     /gene="dhaM"
                     /locus_tag="PK768_03185"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HQA60002.1"
                     /translation="MVGIVLVSHSKKMAEGAKEIITQMIGEKIKIEVAAGTADNRLGT
                     DALLIGQKVLEVFDGDGVLVIPDLGSAVMSSEMAIESLEEPARSATMMADSPFFEGAI
                     AAAMEASFGKSIEEVKKAAENTRGMKKIA"
     gene            7544..7897
                     /locus_tag="PK768_03190"
     CDS             7544..7897
                     /locus_tag="PK768_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQA60003.1"
                     /translation="MDSIFVAVVGVNNYFGTKVFKPGQILRLEKDYDNEYDGEAIMAI
                     LDPIGKVGYVANSTHTVPLGCWSAGRLYDTFESSAFAVVRFVTKETIIAEILENVEFE
                     IYIKEEIFTPEEFED"
BASE COUNT         2585 a         1449 c         1924 g         1958 t
ORIGIN      
        1 cctggcaagg aaatagtcat tctaggttcc ggtgatatag gcttgatcat ggcacgacgc
       61 tttactcttg aaggtgcaaa ggtaaagtgc gttgcggaaa tcatgcccta ttctaatggg
      121 cttacccgca atgttgtcca gtgtcttgaa gacttcggca taccccttct tctgagccac
      181 accgttgtct acattcacgg caatagcagg atagaaggag taactatcgc ccaggtagat
      241 gaaaacttaa agccaattcc ctcaaccgag aaatattatt cttgcgatac tctgctcctc
      301 tcggttggtc tgattccgga aaatgagcta tctaaaatgg cgagcatcaa aattcacgat
      361 gtcaccggtg gacccgttgt aaatcagagg atggaaacgt ccgtccccgg gatttttgcc
      421 tgtggtaatg tagttcatgt ccatgacttg gtggatttcg tgacgatgga atcgagactt
      481 gctggccttg gagcggtgga ttaccttaaa gacaaaatac catcagatga gtatatttca
      541 gtgtctgccg gcccgggaat cagttacgta gtaccccact tagttgatcc tgagaagatt
      601 ttgggtgaaa aacagaattt cttcatgcgc agcaccaaac ccatggaaaa agccaggctt
      661 ttcatagaat cagataagaa tttgattcgc acaagggtac ttcaacatgt aaggcccagc
      721 gaaatgatta gcattgaact gaaaaaagaa gacttggcca ataaagattt aaagagccta
      781 agatttttcc ttcaggaaga aggtgtagct gatggaaact aagaagataa catgtatagt
      841 atgcccaaaa ggttgtcaaa ttcaagttaa gcttaataat ggcgaaattg tcagcataga
      901 aggttacgaa tgcaaacgtg gaattgatta tgcaaaaaat gaagttttag atccaagaag
      961 aacccttacc accaccttaa agctttccac cggtggggtc ctacctgtga ggacaaaaga
     1021 accaatcccc aaaaaattgc tgaaaaaagc catgcttgaa acgaaagata ttatagttga
     1081 gccccccgtt aaagtgggag atgttgtagt tgagaatatt gcggggacgg ggatcgattt
     1141 aatagcaaca ggaaatgtcg aatgatagta ataaaggcag agtgaaaggg tgaaaagcca
     1201 tgcttcaaaa ggatgccgtc tttacccatc ctaaaggaat gcatatcaga gctgctgcca
     1261 tgttggtcaa taaagcacac gagattgaga gcacgatggg aacgagtata tatatacgca
     1321 acgagtccgg aagagaagtg ccggctactg ctctgatggc tattcactcc cttaacatta
     1381 agcccggaga acgcattaca atagtagcaa aagggccaaa agtagatgag gcgatagaaa
     1441 gtgttgaaga actctttaga cgcgatctat tgcctcagag ccctattgaa ttagaacagg
     1501 ttgatgacat attggagaaa aacactataa aagcagacaa gatctttgac agcgtaggcc
     1561 tgggcattat agttaccaat aagacaggcg ccgtaatttt atgcaatcga attgtcgaag
     1621 aattaatggg catagacaaa aatcatattt taggctgcaa taacatacaa gatataattc
     1681 caggcatcga tttgactacc acatcatcta tcggtagtgg aatatttcgc ttggatgaca
     1741 acaccaggag caatctggca tctaccgaca taaacccaat atttatagat ggggaaatag
     1801 ccggctatac aattgtattt aacaaagacg tctccactgc cgaaagcttt tcaccggaat
     1861 ataccttaga gatatctccg accaaagatt tattaataga acaaaagaag agaatggaat
     1921 ttaacgaagc ctttagtgat ataataggtc gcagtgagaa gtttgcggtg gtgctggata
     1981 tagcttttaa ggctgccaag acctcatcga caatcctcat acagggagaa agtggcacag
     2041 gtaaagaact ggtcgctaag gccatacacc gtgccagcag ccgccgcaat ggccctttta
     2101 tcaaggtaaa ctgccccggc ataccggcaa ctctgatgga gagcgaactt ttcggacatg
     2161 aaaggggggc ttttaccggg gcaattttcc aaaaaatagg taagtttgaa cttgcaaatg
     2221 gaggcacaat ttttttggat gaaatcggtg aattagacaa aaacctgcag tccaaactat
     2281 taagagtctt gcaagagcga gaatttgaaa gagttggcgg aaacgaggta ttaaaaaccg
     2341 atgtgagagt tgttgcggca accaatagaa atttgaaaga aatggtagag agagacgaat
     2401 ttcgagaaga cctttattac cgtctcaatg tagtgccgat cgtgctgccg agtttaaggg
     2461 aaagaaaaga cgacattccg cttcttgtgg agcatttttt ggaaaagctc tgcagaaagc
     2521 ttggcaaaaa ggaaaagaca attacaaaaa gagctatggg gtatttgtac aactacgact
     2581 ggccgggcaa tgttcgggaa ctggagaata ttatagagcg gatgataaac ctatccgaca
     2641 gtgacgtaat tgacatttgg gacttgccat ctcatgtaac cggaaaagcc atggaaaaaa
     2701 aagcgagcct tataaattta gacttagatg gagaactggc tacttatgac gagtacgata
     2761 aagcaataat aaaaacggct ttaaaaaagt acaaaagttt caacgcagct ggcaaagccc
     2821 tgggcttaac gcataaaact gtggctgcaa aagcgagaaa gtacggtctt gtaaaataaa
     2881 atattaagaa gggggctgtt ggatggcaaa ttccgggaaa agtattgaca aagtgtcccc
     2941 cgcttctcaa agcgaaatac tgagattaag cgaagagctg ggctatgatg cccatgagat
     3001 aaaatgggtg gttgcacaaa acactgaaac cataagaaat ctagtgaagg taatagaaac
     3061 gatatcatct cattcccaag aaatcagtgc aaatacagct agcggttcgg agcagctcaa
     3121 caacctgtct cgattagcag aaaatttaag agaaactgca tcttctgtag caagtatatc
     3181 aagggactat ttaagcaaag taaaagaagg cagcttagca ataagcgatg tagagacagc
     3241 cttggaacag gtaactgcag aaattgacaa gtcctccgac gaagtagaga ccttacttga
     3301 acttacaaaa caagtaaagg gctttgtaga ttttataaag cacattgcgc agcagacaaa
     3361 tctccttgca ctaaacgctg caattgaagc tgcgagagca ggtgaagctg gacggggatt
     3421 cagcgttgtt gctgctgaaa tcagaaagct tgccgagagg agtaaggata aagctcaaga
     3481 aatacacaaa actgctgaac tcatagacga tggcatcagt agagcgtgtc gcatttctaa
     3541 agatggggca gtaagcctta aaaatctaaa atccaagatg caattgggaa aagatgtgat
     3601 ggaaggcgct gttgccgcct ttgaggatat atcggagctt aacagcaatc tgctggattc
     3661 ttcagcaaat caggcgaagt ctacacagta tctggcagat attttcgcat ctttttcaga
     3721 aaaaacagca tctacagccg actccaccat agaagtggcg tacctgataa aagaccagga
     3781 aaaacaaaac gaacagcttc tcaatattgc tgataattta gttgacaaag tatattcatt
     3841 gcaaaagcaa tccaccagtt taaagaagaa agaggagata atttttggaa taaatcctgc
     3901 cctcagccct gaaattataa aatcattgta ccttcctgtg ataaataccg tttgtaaaaa
     3961 agttggcctc atacctagag tcttggtcgc cactgattac gatgcccttg ccaatagttt
     4021 gatagacggg attgtagatg tgggttggtt ttcgccctta gcctatgtca atgcgaaaaa
     4081 caaggcagcg gttattccct tggttactcc aattgtaaat ggtgctcctg attacttggg
     4141 atatattatc accaatagag aatcgggaat aaccgatttg aaagatgtca gaggagagcg
     4201 tatggccttt gtggacccaa aatctgcttc gggatatgca tatcccaggc ttatcctaaa
     4261 gaaggcaggc atagaccccg aaagggattt aaaagagcag ctctttcttg gaacgcacag
     4321 caatgtagtt caagcagtga tatcgggatc ggttagagta ggtgctacct actcggaagc
     4381 catcgatgat gcgaagagtc gcggccttga agtggacaaa cttgtttatc tggcccagac
     4441 cgacccgata cctaaggatt gcatagcggc acggccgggc tttgataggg aattgtgcga
     4501 aaagctcaag aatgcttttt tgaaatatga ggatataggc ctcttggata agcataaaag
     4561 ttctgtgata aacggttttg tcattgccag tgatgagaat tatgacataa ttcgagaagt
     4621 gttaagagac gctggttgat agaaattacc aactattatt ttgaaatatc tcaaaaaaac
     4681 cgtgtattac agttatttag gatattggca cgatttttgc tttattatca atgcaagatt
     4741 atcttttagg agggatttca tgaaaaagat tattaacagt cctgaagaag tagtacagga
     4801 aatgctggag ggtatggcag cagcatatcc gcagtacgtt cgcaaactgg aaggtttcga
     4861 agttatggta agggctgatg ctccagtaaa aggtaaggtt gcattggtat ccggcggtgg
     4921 cagtggccac gaaccgtctc atgcaggctt tgtaggaaag ggcatgctag atgccggagt
     4981 agccggcgct gtgtttacct cacctactcc agaccaggtt ttcgaagcca ttaaagccgt
     5041 tgacggcggt gcgggtgtcc tgctgatcat caagaactac accggtgaca ttatgaactt
     5101 tgaaatggca ggagaaatgg cagaagctga gggaataaaa gttgccagcg tagtagtaaa
     5161 cgatgacgtt gcggtggaag acagccttta taccactggc aggcgcggaa ttgcgggaac
     5221 ggtatttgtc cataaaattg caggagccaa ggccgaagcc ggtgggacat tggaagaggt
     5281 caaggctgtt gctgaaaaag tcattgcaaa tacaagaagt atgggaatgg ccctcacccc
     5341 atgcatagtg cctgcagcag gcaagcccac ctttacactt ggcgaagacg aaatggaaat
     5401 cggaatgggc attcacggcg agccgggcac aaagcgcaca catatcatga aggctgatga
     5461 actggtagat cacttgatgg aaaagatagt gaatgacctt ccctacgaat ccggagatga
     5521 agtggctgtt atgatcaacg gcctcggcgc aacacctttg atggaacagt ttataatgaa
     5581 tcgccgggta agccagatat taaatgaaaa aggaataaaa gtttatagga cctttgtagg
     5641 agaattcatg acttctattg aaatggcggg agcttcaatt accctgttga aattagacga
     5701 cgagttaaaa gagctattag atgcacaggc cgatacacct gcttttagac aactctgata
     5761 catctgttga aaggggactt aaatgtggct gtaaacagtg caaaactaat tgaaatctta
     5821 aaccttattg caaatgatat acatgcaaac aaagattttt tgacagaact tgactcagcc
     5881 atcggcgatg ccgaccatgg tataaacatg tcaaaaggat tcaaagccgt caccgaaaag
     5941 attgccgcaa tgggcgacaa ggattgtggc agtattttaa aaacggtagg catgacactg
     6001 gtttcgacgg tagggggtgc ttcaggacca ttgtacggta ctgcctttat gcgggcaggc
     6061 caggcaatag gttctaaaac ggaactgggt tttgaagacc tgttggtgac acttgaagca
     6121 gccttggaag gcataaaaat gcgggggaag gctgtaaaag gcgaaaaaac tatagtagac
     6181 gccttggaac ctgcagttga agcattaaag tcttctccaa ccataaatat aaatcttatg
     6241 gaagaggctg tgaaggcagc ggaagctggc gtagagtata ctaagggcat catcgccaaa
     6301 aaagggagag ccagctactt aggtgaaaga agcataggtc atcaagaccc gggagccact
     6361 tcctgccaca tcatgctgac ggcaattctc aaaggcatca aggatagctg actgctacgc
     6421 tggaatgggg tgatgcgttg gtcggtatag ttttagtctc acatagcaaa aagatggccg
     6481 aaggtgctaa ggaaattatt actcaaatga taggagaaaa aataaaaatt gaagtggccg
     6541 ccggaacggc agacaatagg cttggaaccg atgccttatt gatcggacaa aaagtattag
     6601 aggtatttga cggtgatggg gtgttagtta tccctgatct gggcagtgcg gtgatgagtt
     6661 cggaaatggc aattgaaagc ctggaagagc cagccaggtc ggctaccatg atggcggatt
     6721 cacccttctt tgaaggagca attgccgctg ccatggaggc gagtttcggg aaaagcatcg
     6781 aggaggtaaa aaaggcggcc gaaaatacaa gaggcatgaa gaaaatagca taaaaaaaga
     6841 gaattagcca ctccctgtta gagacaatgg cagaattatg atgacgtcaa tgaagtttat
     6901 tgacgtcatt ttttttggga tcccatgcct aaaggataca atttgatggc ttattcatag
     6961 gtcgaagtgc atgggaacca gaaaacttca acaagatcat tcgccaagta ctgccggtgt
     7021 ttttaaaaag gcacaaatct taaaagactg tttcaccagt ctttttttgt ttcatagctc
     7081 aactattcaa agaggatttt ctccccttat accgaaatat tataataacg gtatcgaaaa
     7141 atacagaaaa ttacacataa aaacccacgt atttaaatca aaggtcttat tgatttttat
     7201 tacaaaaatg ttatactacc agagtgattc cgtatttatc taattgatat gtcacaagct
     7261 taaaacagat ttataacgac agtttgttaa agaaaaattt tttttaaaaa ctcatataat
     7321 aagcaataaa agagattaca ttagctttgt agaggccaac accataaaaa cggcatttcc
     7381 attccgaatg gaatacaaaa tggaggattt tgcaacctca gaggagtttg ccttagttaa
     7441 aatgggaaat gatgtggcga tttgcctgga atgaggattg ccgggaactg cggctttggg
     7501 tattatagaa aaaattgttc tttttttata gagggggagt attatggatt cgatttttgt
     7561 ggcagtagta ggtgtaaata attattttgg cacgaaggtt tttaagccgg ggcaaatact
     7621 gcggctggaa aaggactatg ataatgaata cgatggagaa gcaattatgg ccatattaga
     7681 cccaatcggc aaggtgggct acgtggccaa cagcacccat acggttccct tggggtgctg
     7741 gagtgccgga agattgtacg acacatttga atcatctgct tttgcagtgg tgaggtttgt
     7801 gacaaaagag acgattattg ccgagatttt agaaaatgta gaatttgaaa tatatataaa
     7861 agaggagata tttacacccg aggagtttga ggattaaaaa gtatatttga caacaa
//
LOCUS       DAONUO010000056         7909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6972_length_7909_cov_3.879934, whole genome shotgun sequence.
ACCESSION   DAONUO010000056 DAONUO010000000
VERSION     DAONUO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6972_length_7909_cov_3.879934"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /gene="purN"
                     /locus_tag="PK768_03195"
     CDS             complement(<1..450)
                     /gene="purN"
                     /locus_tag="PK768_03195"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQA60004.1"
                     /translation="MTKLRLGVLVSGGGSNLQSIIDHCDSGYLPAEVVIVISSKGGVY
                     ALERAKRHNIPSVVVSPKDYPNRQAYEDEMAKILRSYDVGLVVLAGFLKVLSPHFVNA
                     FQGRIMNIHPSLIPAFCGEGYYGEKVHKAALDYGVKISGVTVHFVDEG"
     gene            complement(447..1472)
                     /gene="purM"
                     /locus_tag="PK768_03200"
     CDS             complement(447..1472)
                     /gene="purM"
                     /locus_tag="PK768_03200"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQA60005.1"
                     /translation="MERLTYKDAGVDVDAGNRAVELIKGHVKSTFRQGVLGEFGGFGG
                     FFQLDVSKYKEPVLVSGTDGVGTKLKIAFMLDKHDTIGIDAVAMCVNDILVSGAEPLF
                     FLDYVAVGKLIPEKLADIVKGVAEGCRIAGCALLGGETAEMPGMYAEDEYDIAGFAVG
                     VVDKMKIIDGSLINNGDVVIGVASSGLHSNGFSLARKVFFEVGKLSPNEHAEELGCTL
                     GEELLKPTKIYSGTVTAIKAFNVRGMAHITGGGFIENLPRILPDGLRIHIDRGTWEVP
                     AVFNMIQKLGSVADEEMFRTFNMGIGYVLIVPQEEADEILKTVLGQGEKAWIIGQVSK
                     GSGGVVF"
     gene            complement(1497..2900)
                     /gene="purF"
                     /locus_tag="PK768_03205"
     CDS             complement(1497..2900)
                     /gene="purF"
                     /locus_tag="PK768_03205"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQA60006.1"
                     /translation="MFDKFKEACGVFGIYRPQVDPALGRSIFYGLYALQHRGQESAGI
                     AVSSGRGIKYHKEMGLVSEVFSDEILEGLSGNVGIGHVRYTSSEANTLINAQPLVVRY
                     KKGSLAVAHNGNLINAHELRRELEDHGAAFQTEIDSEVIAFLIAREHDEDISEAVVNC
                     ADKLKGSYALVIMTEDALIGVRDPNGIRPLCLGKREDSYVLASESCALDTVDAEFIKD
                     VEPGEIVIINKEGIRSIKKRQGGPSSSCIFEFVYFARPDSVIDGRNVHMARWQAGRLL
                     AEEQPADADLVIGVPDSGTVSALGYAEASGIPFGIGLIKNRYVGRTFIKATPMSRAIG
                     VKIKLNAVREVVKGKRLVMVDDSIVRGTTSGLIVNLLKEAGAKEVHVRVSSPTIVHSC
                     YFGIDTSSRKELIGAQRKVEEIREHIGADSLGYLSLEGLIKATGLPADKFCAGCFTGT
                     YPIEVPREGKGYLYEKR"
     gene            complement(2893..5112)
                     /gene="purL"
                     /locus_tag="PK768_03210"
     CDS             complement(2893..5112)
                     /gene="purL"
                     /locus_tag="PK768_03210"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HQA60007.1"
                     /translation="MAGRDEAKKLGLTDLEYDEILNILGREPNFLELTLYSVMWSEHC
                     SYKNSKRVLKTLPTEGPQVLQGPGENAGIVDIGDNLAIVMKVESHNHPSAVEPYQGAA
                     TGAGGVIRDIFAMGARPIALLNSLRFGNLDGERAKYLFKGAVAGIGDYGNAMGVPTIG
                     GEVYFDDAYKENPLVNAMCVGIIRHDKIVRGKAAGVGNSVMIVGAATGRDGMHGASFA
                     SGELSDENPDQGSPMQVGDPFKEKQLMEACLELLEKEWVIGVQDMGAAGVVSSTCETA
                     ARAGSGIELDVALVHQRDENMKPHEIMISESQERMLVIVEKGHEDDVKEIFKKYDLEA
                     SKFGTVTDDGMIRILENGKLVGEVPAKSLAEGVPIVKRESRRPAFLDELNIDISKIPV
                     PEDLNQVLLKLFDSPNIANKAYAYWQFDQTAGTDTVVPPGSDAAVLRIKGSKKGIAVS
                     IDGNGYYCYLDPFEGGKQVVAEAARNLVVTGAKPLALTDGINFGNPEKPEVYYQFEEC
                     VRGISEASLVLGIPVISGNVSFYNEGRETAVYPTPIIGMVGVLDDVSKHCTMGFKKEG
                     DLVVLLGVNTDELGGSAYLKEVLDIEGGPAPRLNLDEELKVQACCLEAIKQGLVSSAH
                     DISDGGLAVALAECCIAGQLGFEGEIESEVRPDALLFGEAQSRIIVSLVEEKLPELKK
                     LAEEMNVKVSVLGTVKSDKNLNISVKTKGQVAGIIDVSIDEIAEISNNAIKRRVEYV"
     gene            complement(5099..5815)
                     /gene="purQ"
                     /locus_tag="PK768_03215"
     CDS             complement(5099..5815)
                     /gene="purQ"
                     /locus_tag="PK768_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQA60008.1"
                     /translation="MRCAVIQFAGSSGDGDCFHVCKNILNKAVEFVFYKESFAPEDFD
                     LIILPGGASHGDYLRPGALAARSKVMKSVIEAAKAKKLVLGIGNGFQILLEVGLLPGA
                     VLMNKGLKFFCDDVFVRVENPDTPFTALYKKGEIIRMPLACKYGNYYADGETIKGLKA
                     DNKIVFSYCDEKGNSTFESNPTGSIENIAGIVNSEGNVLGLMPHPERCAEELLGNTDG
                     LRIFESVVRYIEGGKANGRS"
     gene            complement(5818..6066)
                     /gene="purS"
                     /locus_tag="PK768_03220"
     CDS             complement(5818..6066)
                     /gene="purS"
                     /locus_tag="PK768_03220"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQA60009.1"
                     /translation="MYTVRVYIYYKSGVLDPQSGAIKATLSDLGFDQIHDVETGRFYS
                     LKIDAPNISSAQAIANELCEKLLTNPVIEDYEFEIMEA"
     regulatory      complement(6145..6245)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6569..7567)
                     /locus_tag="PK768_03225"
     CDS             complement(6569..7567)
                     /locus_tag="PK768_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA60010.1"
                     /translation="MANNSSSPLLRIEKLKKHFINKPSLLGRALLKQKDTVLKAVDGV
                     DLDIFSGETIGLVGESGCGKTTLGRTVVKLYKPTSGKIYFKAVDINELTAEKTKEFRK
                     SVQIIFQNPYSSLNPRKTVRDILSAPLILRGINNEVEREEIIKSLLERVGLLARHINS
                     YPHQFSGGQRQRIGIARALALQPEFIVADEPVSALDVSVQAQIINLLEELQEEYNLTY
                     LFIAHDLSVIHYVSNRVAVMYLGKIVEISETLELFNNSLHPYTKALLSAIPRVDRRKR
                     QNRIILEGIVPTPVNIPSGCRFKTRCFMKKGKICDEIEPELIDMGNGHKVACHLYV"
     gene            complement(7560..>7909)
                     /locus_tag="PK768_03230"
     CDS             complement(7560..>7909)
                     /locus_tag="PK768_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60011.1"
                     /translation="LAVAAEFCHEIAVMYAGKIVERGPVDEVMESPKHPYTKGLLESI
                     PKIARVKKKIKAIPGNVPDLADLGDTGCAFYDRCDVAIPKCKETNIPMIEVKTGEFVR
                     CLLYAAKEVATIG"
BASE COUNT         2031 a         2070 c         1482 g         2326 t
ORIGIN      
        1 accttcatcc acaaaatgga ccgtgacacc gcttattttt actccgtaat ccaaagcggc
       61 tttgtgcact ttttcaccgt aatagccttc accgcaaaac gcgggtatca gcgatggatg
      121 aatattcata atcctgccct gaaaggcatt aacaaaatgt ggcgacagga ctttaagaaa
      181 acctgccagc accacaaggc ctacatcgta agaccttagg atttttgcca tctcatcttc
      241 atatgcctgc ctattcggat aatctttagg gcttaccacg acagatggaa tattgtgtct
      301 ttttgccctt tccagggcat aaactcctcc tttactggat ataactatga caacttctgc
      361 cggcaggtaa ccggaatcgc aatggtcaat aatagactga aggtttgaac cgccccctga
      421 taccaatact ccaagcctca gctttgtcaa aataccaccc caccactgcc ttttgaaacc
      481 tgtcctatta tccaggcttt ttcaccctgt cccaagacag tttttaagat ttcatccgcc
      541 tcttcctgtg gtactataag tacatatccg atgcccatgt taaaggtcct gaacatttcc
      601 tcatctgcga cactacccag cttctgaatc atattaaaca cagcagggac ttcccaggta
      661 cctcgatcta tatgaatccg caagccatcg ggcaaaatcc tcgggagatt ttctataaat
      721 cctccgcctg taatgtgtgc catcccgcgg acattaaaag ctttaatggc ggttacggtt
      781 cctgagtaaa tcttggttgg cttcaaaagc tcttccccca aagtacagcc caattcttca
      841 gcatgttcat tgggtgacag cttgccaact tcaaaaaata ctttccttgc cagggaaaag
      901 ccgttgctgt gaagccccga cgatgccaca cctatgacaa catcgccatt atttatcaag
      961 gaaccgtcga tgattttcat tttatctact acacccacag caaagccggc aatatcgtat
     1021 tcatcttcag catacatccc cggcatttca gcggtttcgc cccctaagag ggcacagcct
     1081 gcgatcctac agccttcagc tactcctttt acgatatctg ctagcttttc cgggatgagc
     1141 tttcctacgg ctacatagtc taaaaagaaa aggggctcgg caccgctgac caaaatatca
     1201 tttacacaca tggcaacggc atcgattccg atagtatcgt gcttgtcaag cataaaggca
     1261 atctttaact tggtccccac tccatcggta ccggatacaa gaacgggttc tttgtacttg
     1321 gatacatcga gctgaaaaaa gccgccgaat ccgccgaatt ctcctaatac tccttgccga
     1381 aacgtactct taacatgccc tttaataagc tcgactgcac gattgcccgc atccacatct
     1441 actcctgcat ccttatatgt taatctctcc atatattttt cacacctctc tttgcgctag
     1501 cgcttttcat aaagataacc ctttccctct cgaggcactt ctatcgggta agtgccggta
     1561 aagcacccgg cacaaaactt gtcagcgggt aatcccgtag cctttatgag cccttccaag
     1621 ctgagatagc ccaaactgtc tgctccgata tgctcacgaa tctcttcaac cttgcgctgg
     1681 gcgcctatca gttctttccg tgaagatgtg tcgatgccaa aatagcaaga gtgaacgata
     1741 gtcggcgagc tcaccctgac atgtacctcc ttagcgccgg cctcctttaa aaggtttact
     1801 attaggccgc tggtggttcc cctcactatg gaatcgtcta ccattaccaa acgcttccct
     1861 ttcacgactt cccttacggc atttagcttt atctttacgc caattgccct tgacatgggt
     1921 gtggctttta taaatgtcct gcctacatat cgatttttga taagccctat gccgaaggga
     1981 atgccggaag cctccgcata gcccaaggct gaaaccgtcc ccgaatcagg cacgcctatg
     2041 accaggtcgg catcagccgg ctgttcttct gcaaggagcc tgcctgcttg ccagcgggcc
     2101 atgtgcacat ttcgcccatc tatcacgcta tctggccttg caaaatacac gaattcaaag
     2161 atgcaagagg atgaaggacc gccctgccgc ttcttaatgc tcctgatgcc ttctttgttt
     2221 attatcacta tttcaccggg ctccacatct tttataaatt cagcatccac agtgtcgagg
     2281 gcacagcttt ccgatgcgag gacataggaa tcttcccgtt ttcctagaca tagtggtctg
     2341 atgccgttag gatccctcac ccctatcaaa gcgtcctcag tcatgatgac gagggcgtag
     2401 gaccctttaa gtttgtccgc acagtttacc actgcctctg aaatatcttc gtcgtgttct
     2461 ctagctatta aaaaggcgat gacttcgctg tctatttcag tctgaaaagc cgctccatgg
     2521 tcctccaatt ctctcctgag ctcatgagca tttattagat tgccgttatg ggcaactgcc
     2581 aaagaacctt ttttatagcg gactacaagg ggttgagcgt tgatgagggt attggcttca
     2641 gatgaagtat agcgcacatg ccctatgcca acattgccgg aaaggccttc aagtatctca
     2701 tcactaaaca cttcggagac caagcccatc tccttgtgat atttaattcc cctgccggag
     2761 cttacggcaa ttcccgcact ttcctggccc cgatgctgca gagcataaag gccgtaaaat
     2821 atgctgcggc ctaaagccgg atcaacttgg ggcctatata tgccaaaaac accacatgct
     2881 tctttaaatt tgtcaaacat attccactct ccgctttata gcattatttg aaatttcagc
     2941 gatttcatct atagaaacat caattatccc ggcaacttgg cctttagttt ttacactaat
     3001 atttaaattt ttgtctgact taacagtccc gagcacgcta acttttacgt tcatttcttc
     3061 ggcaagtttc tttaattcgg ggagcttttc ttcgacaaga cttactatga tcctggattg
     3121 agcttctcca aagagcaagg catccggtcg aacttcgctc tctatttcgc cttcaaagcc
     3181 aagctgccct gcaatgcagc actcggccag agcaacggcc aagcccccat cggagatatc
     3241 gtgggcagag cttacaaggc cctgctttat agcttcgagg cagcacgcct ggacttttag
     3301 ctcctcatcg agatttaatc taggtgccgg tcctccctca atgtcaagca cttccttcaa
     3361 atacgcagaa ccgcctaact cgtcggtgtt cacaccgagc aaaaccacca gatctccctc
     3421 ttttttgaaa cccatggtgc agtgcttgct cacatcatcc aaaactccca ccatgcctat
     3481 aatgggtgtg ggataaaccg ccgtttccct gccttcattg taaaaactta cattgccgct
     3541 tataaccggt atgcctaaaa cgagggaagc ttccgatatg cctcttacgc attcctcaaa
     3601 ctgataatat acttccggct tttcaggatt gccgaaatta atgccatctg tgagcgctaa
     3661 gggtttggct ccggtaacta ctaaattcct ggcagcttca gccacaacct gtttgcctcc
     3721 ttcaaaggga tccaggtagc agtagtatcc gtttccatcg atggataccg ctatcccctt
     3781 cttgcttccc tttattctta acacggcagc atcactgccg gggggaacca ccgtatcggt
     3841 gccagcagtt tggtcaaact gccaatacgc ataagctttg tttgcaatat tgggcgaatc
     3901 gaaaagtttc agcaatactt ggttaaggtc ttcaggcacc ggaatctttg atatatcgat
     3961 attcagctcg tcaaggaatg cgggccttct gctctctctt tttacaatcg gcactccttc
     4021 tgctaaagac ttggccggca cttcaccgac aagcttgccg ttttccagta ttctaatcat
     4081 gccgtcatcg gtgaccgttc caaactttga cgcctccagg tcgtatttct taaatatttc
     4141 ctttacgtca tcttcgtgcc ccttttccac gattaccaac attcgctctt gggattcgga
     4201 tatcattatt tcatggggct tcatgttttc atccctctga tgaactaaag ctacatccaa
     4261 ttctatgcca ctgcccgccc tggcggccgt ctcacaggtg gaagacacaa ctccggcagc
     4321 ccccatgtcc tggactccga tcacccattc cttttccaaa agctcgagac atgcttccat
     4381 caactgcttt tccttaaagg gatcccccac ctgcatcggg gagccctggt cggggttttc
     4441 atccgaaagc tcgcctgatg caaagctagc tccgtgcatt ccatctcggc cagtggctgc
     4501 acctactatc atcaccgaat tgcctacccc tgcagctttg ccccttacga tcttgtcatg
     4561 gcgtattatc cccacgcaca tggcatttac cagagggttt tccttgtaag catcgtcaaa
     4621 gtaaacctcg ccacctattg tgggaacgcc catggcattg ccgtaatcgc ctatgccggc
     4681 tacggcacct ttgaaaagat attttgcccg ctcaccgtcc agattgccga atctcaggga
     4741 atttaaaagg gcgatgggcc gtgctcccat ggcgaatata tctcgaataa cacctccggc
     4801 accggtggca gctccttgat agggctcaac tgccgacggg tggttgtggc tctctacctt
     4861 catcacaatg gccaggttgt ctcctatatc cactattccc gcattttctc caggcccctg
     4921 tagcacttgt ggcccttcgg tcggcaaggt ctttaacacc cttttggagt ttttgtaact
     4981 gcagtgttct gaccacatga cgctatataa tgttagttca agaaaattcg gttcccgacc
     5041 caatatattt aatatttcgt catattccaa atcagtaagg cccagttttt tagcttcgtc
     5101 acgacctgcc attagctttc cctccttcga tataccgcac tacggactca aaaattctta
     5161 gaccatcagt attgcccaaa agctcttccg cacaacgctc cggatggggc atcaacccca
     5221 ggacgtttcc ttccgagtta acaatgcctg caatattctc aatagagccc gtaggatttg
     5281 actcaaaagt gctgtttccc ttctcgtcgc aataactaaa aacaattttg ttgtctgcct
     5341 ttaagccttt tattgtttcc ccgtctgcat agtaattacc gtatttacag gctagtggca
     5401 tgcgaattat ctcgcctttt ttgtaaaggg cggtaaaagg agtgtcagga ttctcaaccc
     5461 gaacgaatac atcatcacag aaaaatttta agcctttgtt catcagcact gctcctggaa
     5521 gcaagccgac ctcaagcaat atttgaaatc cgttgccgat tcccagcaca agctttttcg
     5581 ccttagctgc ttcaatcacc gacttcatta cctttgaacg ggcggcaagg gctcccggac
     5641 gaagataatc accgtgagaa gctcctccgg gtagtatgat caagtcaaag tcctccgggg
     5701 caaaggattc cttgtaaaaa acaaactcaa ccgctttatt taaaatattt ttacaaacat
     5761 gaaagcaatc gccgtcaccg ctggaccctg caaattgaat caccgcacac ctcatattta
     5821 ggcctccatt atttcaaatt catagtcttc aatgaccggg tttgtcaaaa gcttttcaca
     5881 tagttcgttg gcaatagctt gggcagaaga aatattcgga gcatcaattt ttaaactgta
     5941 aaatctgccc gtttcaacat catgaatctg atcaaatcca aggtctgata aggttgcttt
     6001 tatggcgccg ctttgaggat ctaatactcc gcttttgtaa tagatataaa ctctaaccgt
     6061 atacattagc ttaaacactc cccttcattt ttataaaaaa tgggtagagc aaatctctac
     6121 ccatagtttg tacgttgtat atagagtgaa agaatttaac cttatagcca ggacggttac
     6181 ggccgcccgg tagagacttt caggccgatt cctgaaatta tataaggttg tcaaaacact
     6241 attctttttt cttatattat cacaagagat gcaaattagc aatactaatc acgaacattt
     6301 taaggtattt tatataaaat gtgcggattt atatattttt aaaccacccg tgtatttgct
     6361 ttcaattttc ggaaacacaa gccactgtag gctactctat gagggcctta agaaatactg
     6421 attaaataag atatgtttaa atgctagtca cttacagtga agcagttgct catgacataa
     6481 aatgattaac agttaagctg caatggtggt agatctgttg gatttgagta tagggaaaaa
     6541 caatttatga aagacaaatg caaatagact atacatataa atgacaggcc accttatggc
     6601 cattccccat atcgattagt tccggttcta tttcatcaca tatttttcct tttttcataa
     6661 aacatcttgt tttaaacctg cagcctgatg ggatatttac tggtgtagga acaattcctt
     6721 caagaattat tctgttttgc cttttcctcc tatcaactct tggtattgct gaaagtaatg
     6781 ctttagtgta ggggtgaaga ctgttattaa acaactcaag ggtttcggaa atttctacaa
     6841 tcttgcccag gtacattaca gcaaccctgt tgctgacata atgaattacg ctcaaatcgt
     6901 gtgcaatgaa taaatacgtc aaattatact cttcctgaag ttcttccaac aaattgatga
     6961 tctgtgcttg gacagataca tcaagggcag aaactggttc atctgcaact ataaattccg
     7021 gctgtagcgc taaagccctt gctataccga ttctctgtcg ctgtcctccg ctaaattggt
     7081 ggggatatga attaatatgt cttgcaagaa ggcctacacg ctctaaaagg ctttttatta
     7141 tttcttccct ctctacctca ttatttatac ctctaaggat gagaggagct gataatatat
     7201 ccctcacggt ctttctggga tttaatgaac tatagggatt ctgaaatatt atctgaactg
     7261 atttccgaaa ctcttttgtc ttttctgccg tgagctcatt tatatctaca gctttaaaat
     7321 agattttacc cgaggtaggt ttatatagct taactaccgt tctccctaat gtcgttttgc
     7381 cacaaccact ttcacctaca aggccgattg tctctccgct gaatatatcc aggtctacac
     7441 cgtcaacagc ctttaatacc gtatcctttt gcttaagcaa agctctaccc aataaacttg
     7501 gtttattaat gaaatgtttt tttaatttct ctattctcaa caacggactg ctactattat
     7561 tagccaattg tagctacctc ctttgcagca tagaggagac atctgacgaa ttctccagtt
     7621 tttacctcta tcattggtat attagtttct ttacattttg ggatagccac atcacatcta
     7681 tcataaaagg cacaacctgt gtcacctaaa tcagccaagt caggcacatt tcccggtatg
     7741 gcctttattt tctttttcac ccttgcaatt ttaggtatcg attccaaaag cccttttgtg
     7801 tatgggtgct tggggctttc catgacttca tcaacgggac ccctttctac aatttttcct
     7861 gcatacatga ccgctatttc atgacaaaat tccgctgcta ctgccaagt
//
LOCUS       DAONUO010000057         7909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_6973_length_7909_cov_3.854214, whole genome shotgun sequence.
ACCESSION   DAONUO010000057 DAONUO010000000
VERSION     DAONUO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6973_length_7909_cov_3.854214"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PK768_03235"
     CDS             complement(<1..491)
                     /locus_tag="PK768_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HQA60012.1"
                     /translation="MELIIADHSGFCFGVKRAMKTIEDLVEKKQKAYTLGPIIHNPQE
                     VERLKNLGITVADFCDIRDGNVVIRTHGVGPDIIEHLQSRGLKIVDCTCPLVKRVQKL
                     AADISGEGYLTIIIGDHNHPEVEGIKSWCSGDAKVIENEEEARNFYTNKKVGIVVQTT
                     QTE"
     gene            complement(608..1195)
                     /locus_tag="PK768_03240"
     CDS             complement(608..1195)
                     /locus_tag="PK768_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQA60013.1"
                     /translation="MFYNTIRFICNVVIHLLFRIKTYGLDNFPKNHAVIVYLNHKSMW
                     DPVIIGCLLKRPIHFMAKEELFYNPLFGYIISKLNAFPVKRGTPDRKAIRKALQVLDD
                     RQVLGIFPEGTRSKDGNLQAPEPGVALIAAKSKDAVLVPAAIIGNYKLFSRIEVRFGK
                     SISFDKYRQGEKMSSSQLKEISADLFTEVAKLMES"
     gene            complement(1185..1844)
                     /gene="cmk"
                     /locus_tag="PK768_03245"
     CDS             complement(1185..1844)
                     /gene="cmk"
                     /locus_tag="PK768_03245"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQA60014.1"
                     /translation="MDFNIAIDGPAGAGKSTVARIIAEKLNMTYIDTGAMYRAITLLA
                     IRHNKRDVKDIIELANQAQIKLSKGSIYINDEDVSDAIRSVDVNSEVSKIARIPEIRL
                     AMVQLQREMAKNKGVVMDGRDIGTTVLPDAKYKFYLTANVKERALRRHREMLAKGEKV
                     TLEDIEKEIIRRDHQDTNRDLSPLKAAEDSIIIDTTDKTVQQVVEDIINIVKRGEGIV
                     L"
     gene            complement(1861..3093)
                     /locus_tag="PK768_03250"
     CDS             complement(1861..3093)
                     /locus_tag="PK768_03250"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA60015.1"
                     /translation="MTKVAVVGGGAAGLMASYQAALNGAKVYLFERNPNLGRKILISG
                     KGRCNLTNIKDIDDFIVHYPGNGKFLYSSILTFSNKDLIAFFEKLGVRTKVERGGRVF
                     PESDSSLDVVRALGKALINSGVNIMYKSRVRHLIIEQKRIKGLVFGESLEEFDCDKVI
                     IATGGLSYPATGSTGDGYKLAQQAGHTVIAPKPSLVPLISQEDWVKDLQGLTLKNVEA
                     TVYLKQIKIASEFGEMLFTHYGISGPIILTLSRYVVDYLEHKPVVSINLKPALTTEEL
                     EQRLMRDFSLYKNKMFKNALNDLLPKKMIPVFIKYTGINPEKKVNQITRNERKKILDS
                     LRYFRVTIVGCREREAIVTRGGVSIKEINPKTMESKIISGLYFAGEVIDIDGVTGGYN
                     LQAAFSTGFVAGQSFALK"
     gene            complement(3106..3567)
                     /locus_tag="PK768_03255"
     CDS             complement(3106..3567)
                     /locus_tag="PK768_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60016.1"
                     /translation="MVKVKLGDDVVQSKIRFKVRFDFKGEHRPGRFLFGGKPIEQVAE
                     ETREEQLALLKNIPVQGISFIDYDISLEPYVVYDEDSGEKVAYAPCEITLNADSIEDM
                     VRFTMREEFRKLEILEPPQMLLTSTDLERILFKMNEEFKNQLLFFLRKIKR"
     gene            complement(3585..4832)
                     /locus_tag="PK768_03260"
     CDS             complement(3585..4832)
                     /locus_tag="PK768_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60017.1"
                     /translation="MPDKILKVEKISFKYDRKNVLNDISFTLEVGDFVGILGPNGCGK
                     TTLLKNINRWLKPDQGTVYIDGSNIFQLSVKDLAKRVATVPQDTSLDVSFRVEQVVSM
                     GRNPHLGSFERERRRDICVVEDAMKTMDVLHLRDKLINQLSGGERQRVLIARALAQEP
                     TLLLLDEPTSHLDINYQWELLELLKNLCTKKSLTILVVLHDINLASMFCNKVILLKEH
                     RIFKTGTLNEVITEQNIKEVFNMNVRVEYPEGRKQPVIIFLNPATEDFHGRPFERLHV
                     ICGGGEGEKLLYYLSQRGYKVTTGVLNKGDTDWKIARLLGFTIVEEAPFSPISEEKIT
                     ENIHQINQSDAVILANIPFGSGNLVNLSCIKQVLAQKKIFILEERDIEERDYTNGKAA
                     QIYNEIKKRATVLASLEDLKKII"
     gene            complement(4844..5668)
                     /gene="speB"
                     /locus_tag="PK768_03265"
     CDS             complement(4844..5668)
                     /gene="speB"
                     /locus_tag="PK768_03265"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HQA60018.1"
                     /translation="MDAKDNYNSSKIVIIGVPMDFTVSFRPGTRFGPKKIREVSYGLE
                     SYSVYADDSLENKSFFDAGDLDIPFGNVEKSLEIIKDVTKQCLKDGKIPLFLGGEHLI
                     SYPIVKQVAEKYPDLTVLHFDAHADLRDSFFGEKLSHATVMRRISEHVKDKHIYQFGI
                     RSGVKEEFVYAEKRTHMFPIEVKAPFLSVLHDIRDKPIYITLDIDVVDPAFAPGTGTP
                     EPGGCSSQEIVEITRYFEGLNIVGFDLVEVSPANDLSERTSLLAAKILRELIIALG"
     gene            complement(5710..6543)
                     /gene="speE"
                     /locus_tag="PK768_03270"
     CDS             complement(5710..6543)
                     /gene="speE"
                     /locus_tag="PK768_03270"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HQA60019.1"
                     /translation="MELWFSEYQTKNVKLSFRIKEVLYTKQSSYQKIAVYETEDFGRI
                     LTLDDTIMLTTKDEYIYHEMISHVPMLTHGDASRVLVIGGGDGGTVRELLKHPVKEIH
                     LVEIDKEVIETSKKYFPTISCGLSDPRVKIYFEDGIEFVKKNTGYDVVIVDSTDPIGP
                     AVGLFSAEFYKNVFEALNEDGILVAQTESPILFDNLVKKIYKDMSSVFPYTNMYSAVI
                     PTYPGALWTFTMGSKTINPLLKEVSSLPHIDTKFYRRELQKSYFILPPFVSNLISDRG
                     V"
     gene            complement(6639..7094)
                     /locus_tag="PK768_03275"
     CDS             complement(6639..7094)
                     /locus_tag="PK768_03275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQA60020.1"
                     /translation="MITIRKAIADDKLTIQKLNNNADVRQFCLDCLPEHTMVVELEDT
                     LVGYGSLDIADELAIIRCVYIAHNYRNQGLGDGLVRALINYADRRAVKRIYIFSDREI
                     DYFKRFGFKYISSDQCDIKIVGAYNTNSDDYSYIMELDIDEYFNSRHCH"
     gene            complement(7091..7822)
                     /locus_tag="PK768_03280"
     CDS             complement(7091..7822)
                     /locus_tag="PK768_03280"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQA60021.1"
                     /translation="MQIRLQKFLSQAGIASRRASETLILDGRVTVNGITVKELGTKVN
                     PESDIIEVDGKICNIKDDYIYILLYKPKGILTSVKDPFKRPTVIDLLKGIKEKVVPVG
                     RLDKDTEGLLLLTNDGDLTYKITHPRFEVVKTYIARVEGTIDEKDIKALEKGIMLEDG
                     LTSPAKVRIIKKFDKSTVIELKIHEGRKRQVRRMCDAIGHPIISLKRTQIGRLSLKGL
                     KVGQWRYLNQEEMNYIKRLKGEGSD"
BASE COUNT         2236 a         1722 c         1247 g         2704 t
ORIGIN      
        1 tcttcggtct gagtggtttg gactactatg ccaacctttt tatttgtata aaagtttcta
       61 gcttcctctt cgttttctat tacttttgca tctccactgc accagctctt tataccttca
      121 acttccggat gattatgatc acctatgata atagtgaggt atccctcacc ggaaatatct
      181 gcggctagtt tttgaaccct ttttacaagg ggacatgtac aatcaacaat ctttaagcca
      241 cgcgattgta aatgttctat tatatccggg ccaacaccgt gggtcctgat taccacattg
      301 ccatctctaa tatcacaaaa gtccgcaact gttatcccta gattcttaag tctctccact
      361 tcttgagggt tgtgaattat cgggccaaga gtgtaagcct tttgtttttt ctcaactaaa
      421 tcctcgattg tcttcatggc tctctttacg ccaaaacaaa atcccgaatg atcggcaatt
      481 attaactcca aaagattcac atcctactaa aattattatt cgcatataaa aacaattgat
      541 tcttgcagag tccactaact ttaaattgat tctataaaga tatcgataat tcctgctttt
      601 tatcaaatta tgattccatt aattttgcta cttcagtaaa aaggtcagca cttatctcct
      661 ttaattggct ggaactcatc ttttcacctt gacggtattt gtcgaagctt atggatttgc
      721 caaatctcac ttcaatcctt gaaaataatt tgtagttccc aattattgct gcaggaacca
      781 gcacagcatc tttactctta gcagcgataa gtgccactcc cggttccggt gcctgcaagt
      841 tgccatcttt actcctggta ccctctggaa atattcccaa aacttgcctg tcatctaata
      901 cttgcagggc ttttctaata gctttcctgt caggcgttcc ccttttaacg ggaaaagcat
      961 ttaatttgct aatgatatat ccaaacagag gattataaaa cagctcttcc tttgccataa
     1021 aatggattgg cctttttaag agacaaccga taattacagg atcccacatg cttttatgat
     1081 ttaagtaaac aatgactgca tgatttttgg ggaaattatc taggccatat gtcttgattc
     1141 taaagaggag atgtataact acattacata taaatcgaat tgtattataa aacaatgcct
     1201 tccccccttt tgacaatatt aataatatct tcaactacct gctgcacggt tttgtcagta
     1261 gtatcaatta ttatagagtc ttctgccgcc tttaaaggag ataaatcacg atttgtatcc
     1321 tgatggtccc tccgtattat ttctttttca atgtcttcta atgtaacctt ttcacctttt
     1381 gcaagcatct ccctatgtct tcgtaatgct ctctctttaa cgtttgcagt taaatagaac
     1441 ttgtactttg catcaggcaa aacagtagtt ccgatatctc ttccatccat cacaacacct
     1501 ttgtttttag ccatttctcg ttgcagttgt accatagcaa gcctaatttc aggtattctt
     1561 gctatttttg aaacttcact atttacatca acacttctga tagcgtccga tacatcttca
     1621 tcgtttatat aaatactgcc tttcgacagt tttatttgcg cttgattagc cagttcaatt
     1681 atatctttca catcacgttt attgtgacgg attgcaagta atgttattgc cctgtacata
     1741 gctcctgtat caatataagt catgtttaac ttttcagcaa tgattctggc aacggtgctt
     1801 ttgccagcac ctgcaggtcc atcaatagca atattaaaat ccaatagaat ctccccttat
     1861 tcattttaag gcaaagctct gccctgcaac aaatccggta gaaaaggcag cttgtaggtt
     1921 ataacctcca gttactccat ctatgtcaat aacttcaccg gcaaagtata agccgcttat
     1981 tatctttgat tccattgtct taggattgat ctctttgatt gaaactcctc cacgggtaac
     2041 tatggcttct ctttctctac atccgacgat agtcacacga aaatatcgta aagaatccag
     2101 tatcttcttt ctctcattgc gggtaatttg gtttaccttt ttttccggat taattcccgt
     2161 atatttaata aaaaccggaa tcatcttttt aggcaataaa tcgtttaatg catttttaaa
     2221 cattttattt ttatatagac taaaatctct catcagcctt tgctcaagtt cctctgttgt
     2281 taaagccggc tttagattta tggaaactac gggcttgtgt tccagataat caaccacata
     2341 tctgcttaag gtcagtataa tgggcccgga aattccataa tgggtaaata acatttcgcc
     2401 aaattcggag gctatcttta tttgttttaa atataccgtt gcttcaacat ttttcaacgt
     2461 aagaccttgc aggtctttta cccaatcttc ttgggaaatt aaagggacca gtgatggctt
     2521 aggagctatt acggtgtgac ctgcttgctg agctagtttg tacccatctc cagtagaacc
     2581 tgtagcggga taagaaagtc ctccagttgc aataataacc ttgtcacaat caaactcttc
     2641 aagactttcg ccgaatacaa ggccttttat tcgcttttgt tcaatgatta gatgccttac
     2701 tcgacttttg tacattatgt ttacacctga attaattaga gcctttccaa gtgctcttac
     2761 cacatccagg gagctatcgg actcgggaaa taccctgcca ccccgttcta cttttgttct
     2821 aaccccaagt ttttcaaaaa aggctattaa atccttgttc gaaaatgtca aaatggaact
     2881 atagagaaac ttgccatttc ccggataatg gacaatgaaa tcatctatat cttttatgtt
     2941 cgttaaatta catcggcctt ttccggaaat cagtatcttt ctgcccaaat ttggattcct
     3001 ctcaaaaaga tatacttttg caccatttag agcggcttgg taagatgcca taagaccggc
     3061 agcaccaccg ccgacaactg caactttagt catccaatca tcccattacc gttttatttt
     3121 tcgcaagaaa aacaaaagct gatttttaaa ttcttcgttc attttaaata atattctctc
     3181 taagtcggta ctcgttaaaa gcatttgggg aggctccagt atttctagct ttcgaaattc
     3241 ttccctcatg gtgaaacgaa ccatatcttc tatcgaatct gcattcagtg ttatctcgca
     3301 aggagcatat gccacttttt ctccagaatc ctcatcataa accacatagg gctccagtga
     3361 gatatcatag tctataaaag aaataccctg aacgggtata ttttttagca aggctaattg
     3421 ttcttcccgt gtctcttcag caacttgttc aatgggctta ccaccaaata aaaaccttcc
     3481 tggcctatgt tcacccttaa aatcaaatct taccttaaac ctgattttac tttggactac
     3541 atcatcacca agtttcacct ttaccatttg ttggccctcc aatactaaat tatctttttt
     3601 aagtcttcca aagaagcaag aaccgttgct cttttcttga tttcattgta tatttgcgca
     3661 gcttttccgt tagtgtagtc tctctcttca atatctcttt cttccagtat aaaaatcttt
     3721 ttttgagcaa gcacttgctt tatacaggaa agatttacta aattcccgga gccaaaggga
     3781 atgttagcca ggataactgc atcggattga ttaatctgat gaatgttttc tgttatcttc
     3841 tcctcagata tgggcgaaaa gggagcttcc tcaacaatgg taaaacccaa aagccttgct
     3901 attttccaat cggtatcccc tttgtttaaa actccagtgg taaccttata acctctttgg
     3961 ctcaaataat ataacagttt ttctccttct ccaccgccac atattacgtg aagtctttca
     4021 aagggccttc cgtgaaaatc ttcagttgcc ggattcagga agataatgac gggctgcttt
     4081 ctgccctcag gatattctac ccggacattc atgttgaaaa cttctttaat attttgctca
     4141 gttatcactt catttaaggt gcccgtttta aatattctat gttcttttaa gagaatcact
     4201 ttgttgcaga acatagatgc taaattaatg tcgtgaagca caaccaatat cgtcaaggat
     4261 ttttttgtgc acaaattctt aagtagctcc aaaagctccc attgataatt tatatctaaa
     4321 tgtgaagtgg gttcatctaa taaaagtaga gttggttcct gtgcaagggc tcttgcaatt
     4381 agaacccttt gcctttcccc gccgcttaac tgattgatga gcttgtcgcg caggtgcaac
     4441 acatccatag ttttcattgc atcttcgact acgcatatat ctcgcctgcg ttctcgttca
     4501 aaacttccca gatgaggatt tctacccatt gaaactacct gttctaccct aaaactcaca
     4561 tctaaagatg tgtcctgagg aaccgtcgct acgcgcttag ccaaatcctt gacgcttagc
     4621 tgaaatatat ttgaaccatc tatataaaca gttccttgat ctggtttaag ccagcgatta
     4681 atgtttttta acaaggtggt cttgccgcaa ccgttaggcc ccaatatgcc tacaaaatcg
     4741 ccaacttcta gcgtaaagct gatatcattt aaaacgtttt ttctgtcata tttaaaactg
     4801 atcttttcaa cttttaaaat tttatcgggc attttatcac cttttaccct aaggcaatta
     4861 ttagttcccg aagtattttt gctgccaaga gggaagtcct ttcagataag tcattggctg
     4921 gagaaacttc tactaagtca aagccaacaa tgtttaatcc ttcaaaatac cttgttattt
     4981 ctactatctc ctgggaactg cacccacccg gttcaggagt tcctgtacca ggtgcaaaag
     5041 ctggatctac aacatcaata tcaagggtaa tgtatatagg tttatcccta atatcatgta
     5101 atacacttaa aaaaggagct tttacttcta ttggaaacat atgggtgcgt ttttctgcat
     5161 agacaaactc ctcttttact cctgaacgta ttccaaattg ataaatatgt ttatctttaa
     5221 catgttcaga tattctgcgc atgaccgtag cgtgggaaag cttttctcca aaaaaactgt
     5281 ctcttaaatc agcgtgggcg tcaaaatgca aaacagtaag atcagggtat ttttcagcta
     5341 cctgtttaac aataggatag ctaatgaggt gttcccctcc caaaaacaag ggaattttgc
     5401 cgtcctttaa gcactgcttt gtgacatctt ttataatttc aaggcttttt tcaacattgc
     5461 caaaaggtat gtccaaatct cctgcatcaa aaaaactctt gttttcaagg gaatcgtcag
     5521 cgtacacgct gtaactttca agaccgtagg atacttccct tattttctta gggccgaatc
     5581 tagttccggg tctgaaactt actgtaaagt ccatgggaac tccgataatt actattttgg
     5641 agctattgta attgtctttt gcatccataa atcggtcctg attgtgtaag tgttcaaaaa
     5701 tattcaagtc tacacacctc tatcagagat taaattactt acgaaaggtg gcaaaataaa
     5761 atagctcttt tgaagctctc ttctatagaa tttggtgtca atatgcggca aagagctgac
     5821 ttcttttaaa agaggattta tggtttttga acccatagta aaggtccaaa gagctcccgg
     5881 ataggtggga ataactgctg aatacatatt ggtataagga aacacggaag acatatcctt
     5941 atatattttt ttaactaaat tgtcaaacaa aataggtgat tctgtttgag ccaccaaaat
     6001 tccatcttca ttcaatgctt caaagacgtt tttataaaac tctgcagaaa acaggcctac
     6061 agctggacct atgggatcag tagaatctac tattaccaca tcatagcctg tattcttttt
     6121 tacgaattca attccgtctt caaagtaaat ctttactctt ggatccgata agccacaact
     6181 gattgttggg aaatattttt tcgaagtctc tatgacttcc ttatctattt caactaagtg
     6241 gatttccttt actggatgtt tcaatagctc tcttaccgta cccccatctc caccgccgat
     6301 aaccaaaacc ctcgatgcat cgccatgtgt cagcatgggc acatgtgaaa tcatctcatg
     6361 ataaatgtac tcgtccttag ttgtaagcat tatagtatcg tctagggtca aaatcctgcc
     6421 aaagtcttcg gtctcgtaaa cagctatttt ttggtacgag ctttgctttg tataaaggac
     6481 ttcttttatt ctgaaagata actttacatt ttttgtctga tattctgaaa accagagctc
     6541 caagcgcttc acccctaatt ttaatatatt tttccgggaa aattatatca gataaaaact
     6601 aaattctcaa tcagataatt aagcgtcata taccatagtt aatggcagtg gcgactgtta
     6661 aagtattcat ctatatccaa ttccattatg tatgaataat cgtcagaatt cgtattatag
     6721 gcccctacta ttttaatgtc acattggtca gatgagatat atttaaaacc gaatcgctta
     6781 aaataatcta tttcacggtc actgaatata tatatccttt taacagctct gcggtcagca
     6841 taatttataa gtgccctgac taatccatct cctaatccct gattgcgata gttatgcgca
     6901 atatatacgc atcttataat agcaagttca tcggcaatat ccaaagatcc gtaacctacc
     6961 agggtatctt caagttccac gaccatagta tgttccggaa gacaatcgag gcaaaattgc
     7021 cttacatctg cattgttatt aagtttctgt attgtaagtt tgtcatctgc aattgccttt
     7081 cttattgtaa tcaatcgctt ccctctcctt taagtctctt tatataattc atttcttcct
     7141 gatttaaata cctccactgg cctactttta acccttttaa cgatagtctt cctatttgcg
     7201 ttctctttaa actgataata gggtgtccaa tggcatcaca cattcgcctg acctgtcgct
     7261 ttcttccttc atgtatcttt agttcaatta cagtggattt gtcgaacttc tttattattc
     7321 tcacttttgc aggtgaagtt aagccatctt caagcatgat tcccttttct aatgctttga
     7381 tatctttttc atcaatagtg ccttcaacgc gagcaatata tgttttaacg acttcaaatc
     7441 taggatgagt aatcttatat gtaaggtcac catcattagt caatagcaag agaccctctg
     7501 tatctttatc caggcggcca acggggacaa ctttttcctt tattcccttt aataagtcaa
     7561 ttacagtagg ccttttgaag ggatccttta cactggtgag tattcccttc ggtttgtata
     7621 agagtatata tatataatca tcctttatat tgcaaatttt gccgtctacc tcaattatat
     7681 cagattccgg attcactttt gttcccaatt ctttaactgt gattccgttt actgttactc
     7741 gcccatctaa tataagtgtt tcactggccc tccgcgaagc aatccccgct tgagataaga
     7801 atttttgcaa tcgtatttgc aactataact ccccttttat ctctaatatt aaacatatat
     7861 aataaagtat ataaaaaatt gtactattac caagcatgtc tataatagc
//
LOCUS       DAONUO010000058         7862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7017_length_7862_cov_3.466120, whole genome shotgun sequence.
ACCESSION   DAONUO010000058 DAONUO010000000
VERSION     DAONUO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_7862_cov_3.466120"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /gene="msrA"
                     /locus_tag="PK768_03285"
     CDS             <1..426
                     /gene="msrA"
                     /locus_tag="PK768_03285"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQA60022.1"
                     /translation="FANGRTDNPTYEDVVQKNTGYAETVKVTYDAEKLDLNLLLDLYF
                     LAIDPTSVNKQGNDIGDQYRTGIYYTSGQQLPLINQRINKEAAKHIRAIAVEVTPIKK
                     YYKAEDYHQDYLDKNPGGYCHINPALFKVAKEANKATNK"
     gene            455..1450
                     /locus_tag="PK768_03290"
     CDS             455..1450
                     /locus_tag="PK768_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HQA60023.1"
                     /translation="MKKVAINGFGRIGRAALKLIFETDDLEVVAINDLMSVENAAYLL
                     KYDSNYGKYAKDVKFEGDSLFVGDKEIKYSSLRNILELPWKALNVDVVIESTGIFTNS
                     ADAEGHILAGAKAAVISGPTKDTPTVVYGVNTTDDKTLVFSCASCTTNNISPVIEILG
                     RRLGIKKAILNTIHGYTASQSLVDAPSKKEPRMGRAAGLNLAPAATGAAIATTKVLPQ
                     YAGKFDGVAIRVPVPVGSISDITFIAERPTTVEEINQILSEEATTERYKKVIAVTNEP
                     LVSTDIIGSAFASTVDLEMTRVVDGDLVKIMAWYDNEWGFTNQMIRQIQSIWQPE"
     gene            1517..1987
                     /locus_tag="PK768_03295"
     CDS             1517..1987
                     /locus_tag="PK768_03295"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQA60024.1"
                     /translation="MIFIVMEILNNQIQPWHLESIGQKDLPHYDSFKGKPLLILFFYL
                     GCPGCVGRAIPFANKMAYEHGDKMNVLGIHSNFEGPEYTNDEIIENLKSLHVRFPVFR
                     DAGMATTFHDYQAGGTPHWVLVDKNGKVVRSIFGSDPNRALLWLDYAIIEELEK"
     gene            2032..3513
                     /gene="cysS"
                     /locus_tag="PK768_03300"
     CDS             2032..3513
                     /gene="cysS"
                     /locus_tag="PK768_03300"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQA60025.1"
                     /translation="MTIYKDHPLKIYNSLSNEKEIFTPIQDGHVGMYVCGPTVYSNVH
                     LGNVRTFLSFDIIYRYLLHLGYKVRYVRNITDVGHLVDDADSGEDKIAKRARLEQLEP
                     MEIVQRYTVDFHDILQKFNLLPPSIEPTATGHMIEQINVIQNIIDKGYAYESNGSVYF
                     DVAKYSEDYDYGVLSHRDLIHTLDNSRPLDGQSDKRNEQDFALWKRADPHHIMRWPSP
                     WSDGFPGWHTECTTMSTKYLGQRFDIHGGGMDLKFPHHECEIAQGEASTGHAPVNYWL
                     HANMLTLNGKKMSKSSGNNILPDEILAGDNPVLSKAFSASVTRFFMLQTHYRSIMDFS
                     NEAILAAEKGYDRLMKAINSLSKLEPGNRSTLDIAGWEAKCYEALDDDFNTPILISHL
                     FEGVTYINQLKDKTAILSATDLEHFNKGISTFVFDVLGLEMRSGVYDSYAEKLDGTIK
                     LLIEIRNKVRADRNFTLSDQIRNRLAELAIKLNDSKEETTFNY"
     gene            3595..4428
                     /locus_tag="PK768_03305"
     CDS             3595..4428
                     /locus_tag="PK768_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA60026.1"
                     /translation="MNINMIYQGKQNEYFELEYVDCDNFRSFRPIVSGALQLLWFQSD
                     GNKLIIDGIHQTFNNNQIVSLSQYHHVQYEQINAMKMLRFNSEFYCIINHDSEVGCKG
                     VLYYTPAKIPVVSVQEPQFAIMNDAWNLTDMELEMKDELQLEMLQIVLKRILILCTRI
                     YKMQGTLSFIDDSQHNLIREFNFLVEQNFKEQHSVSYYAGLLHKSPKTITNTFKKIDE
                     KSPLQLIHDRILLEAKNLLHYTKRDISEIAYELGFENVQVFSRFFKRLSGISPTEFRL
                     K"
     assembly_gap    4813..4822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5056..5304
                     /locus_tag="PK768_03310"
     CDS             5056..5304
                     /locus_tag="PK768_03310"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HQA60027.1"
                     /translation="MTLSFDKTVIEAAKAYAEEQGISLSRLTEYLYKQITRKESQSLE
                     SLPIADWVLQIAEGAAEYKTKPRSRKANRAEYFDSRKK"
     gene            5316..5615
                     /locus_tag="PK768_03315"
     CDS             5316..5615
                     /locus_tag="PK768_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60028.1"
                     /translation="MDANLLVAVLNKEYPAFTDAARVLSLAGQKGFVLYTSPLCLADK
                     KVGDLEDRLEYYAAEKAGCTHLLTNDTGDYHFARLPVYTPEAFLREIIGRNDLFA"
     gene            5718..6161
                     /gene="rplK"
                     /locus_tag="PK768_03320"
     CDS             5718..6161
                     /gene="rplK"
                     /locus_tag="PK768_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQA60029.1"
                     /translation="MAKEISGFVKLQCKGGQANPAPPIGPALGSKGVNIMEFCKQFNA
                     RTQDKMGKVLPVLITVYSDKSFDFIIKTPPASVQLLEAAKLQGGSKEPNRSKVGKVTW
                     AQVEAIAKDKMPDLNCFTVESAMKMVAGTARSMGITVDGVAPWEN"
     gene            6188..6880
                     /gene="rplA"
                     /locus_tag="PK768_03325"
     CDS             6188..6880
                     /gene="rplA"
                     /locus_tag="PK768_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQA60030.1"
                     /translation="MAITKKRKAVNSKVDAAKIYSLKEASSLVKEVNTTKFDASVDLH
                     IRLGVDPKKADQAVRGTVTLPHGTGKTKKVLVLCPADKENDAKNAGADFVGLDEFITK
                     IEGGWTDVDVIIATPSVMPKIGKLGKVLGPRNLMPNPKTGTVTNDVAAAVNEVKGGKI
                     AFKVDKAGIVHASIGRVSFNADKIAENSQELINALIKLKPATAKGTYLKGVSMASSMS
                     PGITIDTKLFIH"
     gene            6898..7434
                     /gene="rplJ"
                     /locus_tag="PK768_03330"
     CDS             6898..7434
                     /gene="rplJ"
                     /locus_tag="PK768_03330"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQA60031.1"
                     /translation="MTKEQKQEVIELLKGKFSQYSNFYITDTESLSVDQVSKLRRHCF
                     NKQVEMKVAKNTLIKKALEQIDAEKYSGVYAALNNVTALLFSENPKDPAVILTSFRAD
                     EKSERPVLKAAFINGDIYVGDDQLKSLTKIKTKNELIGEVIGLLQSPAKRVIAALLEK
                     ANKEGGAEAAPATAAAAE"
     gene            7532..>7862
                     /gene="rplL"
                     /locus_tag="PK768_03335"
     CDS             7532..>7862
                     /gene="rplL"
                     /locus_tag="PK768_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQA60032.1"
                     /translation="MADVKSLAEQLVGLTVKEVQELADVLKSEYGIEPAAAAVVVAAD
                     GGAGAAAVEEKTAFNVILKSGGASKLNVVKIVKDLTGLGLKEAKELVDGAPKAVKEGV
                     SKAEADDI"
BASE COUNT         2481 a         1595 c         1726 g         2050 t
ORIGIN      
        1 tttgccaatg gccgtaccga caatccgaca tatgaggatg tagtacagaa aaatacgggc
       61 tacgctgaaa ccgtaaaggt tacatacgat gctgaaaaat tggatttgaa tctgttactt
      121 gatttgtatt tccttgctat tgatcctacg agcgtaaata aacagggtaa tgatatcggt
      181 gatcagtacc gtacgggcat ttattacacc tcaggccagc agttaccact tataaaccaa
      241 cggataaata aagaggcggc aaaacatatc agagctatag cagttgaagt aacccccata
      301 aagaaatatt ataaagccga ggattaccat caagactacc tggataagaa tccgggagga
      361 tactgccata ttaatccggc actttttaaa gtggcgaagg aagctaataa ggcaaccaat
      421 aaatgataat atcatcaaaa aactataaaa aatcatgaaa aaagtagcaa tcaacggatt
      481 tggtcgcata ggaagggcag cattaaaact gatttttgaa accgatgacc ttgaagtcgt
      541 cgccataaac gatttgatgt cggtagaaaa tgcagcatat ctgcttaaat acgacagtaa
      601 ctatggtaag tatgctaaag atgttaaatt tgaaggagac agcttatttg tgggggataa
      661 agaaatcaag tattccagtc tacgcaacat tttagagtta ccatggaaag cactcaatgt
      721 agacgttgtt attgaaagta ccggcatatt taccaacagt gccgatgctg aagggcatat
      781 tctggctggt gctaaagcag ctgtaatttc aggtcctaca aaagacactc caacagttgt
      841 ttatggcgta aatactacag atgataagac attggtcttt tcttgtgcaa gttgtacgac
      901 caataacatc agccctgtaa tagaaatttt gggaagaagg ttaggtataa aaaaagcaat
      961 tcttaatacc atacatggat atactgcttc acaaagcctt gtcgatgcgc cttcgaaaaa
     1021 agagccacgc atgggtagag cggccggctt gaatctggca ccggctgcta cgggagcagc
     1081 catagcgact actaaagtat tacctcagta cgccggaaag ttcgacggcg ttgccattag
     1141 agttcccgtc cctgttggct ccatttcgga tattactttt attgctgaga gacctaccac
     1201 ggttgaggaa atcaatcaga ttctgtcaga agaagcgacc acagaacggt ataaaaaagt
     1261 gatcgctgta acaaatgaac cccttgtatc aactgatatc ataggaagtg ctttcgcttc
     1321 taccgtagat ttagaaatga caagggttgt tgatggagac ctagtgaaaa taatggcttg
     1381 gtatgacaat gaatggggat ttaccaatca gatgatcaga cagattcaat caatatggca
     1441 acctgaataa actgatttaa gcgcacatgc ttacgtcgat taactgcatt ccccttctga
     1501 aaaggatagg ggaattttga tatttattgt tatggaaatt ttgaacaacc aaatccaacc
     1561 ctggcatttg gaaagtatcg gacagaaaga tcttccacat tacgatagct tcaaaggaaa
     1621 acccttattg atcttgtttt tctatttagg ctgtccggga tgtgtaggaa gagctatccc
     1681 atttgccaat aaaatggcgt atgaacacgg ggacaagatg aacgtattag ggattcatag
     1741 caattttgaa ggtcccgaat ataccaatga tgaaattatt gaaaatttaa agtcattgca
     1801 tgttaggttt ccggttttta gggatgccgg tatggccacc acttttcacg attaccaagc
     1861 cggggggaca cctcactggg ttttggtaga taaaaacggt aaggtagtcc gttccatatt
     1921 tggttcggat cccaatcgag cattattatg gttagattac gcaattattg aagagttgga
     1981 aaagtgaatt agaatttacc ttcaaaacat tgcaactttt gaatattcaa tatgactata
     2041 tataaagatc atccactgaa aatatataac tcactttcga acgaaaaaga aatattcaca
     2101 ccgattcaag atggtcacgt tgggatgtat gtctgcggtc cgacggtgta cagtaatgta
     2161 catcttggta atgtaaggac attcctctcg ttcgatatca tctatcgcta tcttctgcat
     2221 ttaggctata aagtccgtta tgtgcgtaat atcactgatg taggacacct ggttgatgat
     2281 gcagatagcg gtgaagataa aattgcaaaa agagcgcgtt tggaacaact tgaaccaatg
     2341 gaaattgttc aacgctatac cgttgatttc catgatatcc tccaaaaatt caaccttttg
     2401 ccgccaagca ttgaaccaac ggcgaccggt cacatgattg agcaaatcaa tgtcatacag
     2461 aacatcatag ataagggcta tgcctacgaa tcgaatggat cggtatattt cgacgtggca
     2521 aagtatagtg aggattacga ttatggtgtg ctcagccatc gggacctgat acacacgctt
     2581 gataactcca gaccacttga cggccagtct gacaaacgga atgaacagga ttttgcgcta
     2641 tggaagcgtg ctgatccaca ccatataatg cgctggcctt cgccgtggag cgatggtttt
     2701 ccgggatggc atacggagtg caccacaatg agcacaaaat accttgggca aagatttgac
     2761 atccacggcg gaggaatgga tctgaaattt ccacatcatg aatgtgaaat agctcaggga
     2821 gaagcctcca cgggccatgc accggttaac tactggctgc atgccaatat gttaacgctg
     2881 aatggaaaga aaatgtccaa atctagtgga aataatattc tgccagatga gattttggct
     2941 ggcgacaatc ctgttcttag caaagcgttt tcagcttcgg tgacgcgttt ttttatgtta
     3001 caaacgcatt atcgaagtat tatggatttt tcgaatgagg cgatcctggc tgctgaaaaa
     3061 ggatacgacc gcctgatgaa agcgataaat agcttatcga agcttgagcc tggaaataga
     3121 tcgacactcg acatcgctgg ttgggaagcc aaatgttatg aggcgttgga tgacgatttt
     3181 aatacgccga tacttatctc acaccttttt gaaggcgtta cgtatattaa tcaactgaaa
     3241 gacaagactg ccatactttc agcaaccgat ctggaacatt tcaataaagg tatttcaacc
     3301 tttgtttttg acgtactagg attggaaatg agatcgggtg tgtatgacag ctacgctgaa
     3361 aaactcgacg ggacaatcaa attactaatt gaaattagga ataaggtgcg agcagataga
     3421 aactttactt tgtcagatca gatacgcaac cgactcgctg agttagctat caaattgaat
     3481 gattcaaagg aagaaacaac ttttaactat tgatctgatt taaagattcg ctttaaatta
     3541 aattgtgttt tgagattata tgtatttttg ataataatat agaagtactt taaattgaat
     3601 attaatatga tctatcaagg aaagcagaac gaatactttg agttggagta tgtagattgt
     3661 gacaatttca gaagctttag gccgattgta tctggggctc tgcagctact gtggtttcaa
     3721 tctgacggaa acaaattgat catcgatggc attcaccaaa cttttaataa caaccagatt
     3781 gttagtctct ctcaatacca tcatgtgcaa tatgagcaga ttaacgctat gaaaatgctt
     3841 cggttcaatt cagaattcta ttgcataatt aaccatgata gcgaagtcgg ctgtaaaggt
     3901 gtattgtatt atacacctgc aaaaataccg gttgtttcgg ttcaggagcc gcaatttgcg
     3961 ataatgaatg atgcctggaa cctaaccgat atggaattag aaatgaaaga cgaattgcag
     4021 cttgaaatgc tgcaaattgt cctcaagcgc atcctgatac tgtgcacaag aatatacaaa
     4081 atgcaaggta ccttgtcttt catcgacgat agtcagcata atttaataag ggaattcaat
     4141 tttctggttg aacaaaactt taaggagcag catagcgtct cttactatgc aggtttatta
     4201 cacaaatcac caaagaccat taccaatacc tttaagaaga ttgatgaaaa atcaccactt
     4261 cagcttatcc atgaccgtat tctgttagag gctaaaaact tgctgcatta tacaaaacgg
     4321 gatatttctg aaatagcgta cgaactcgga tttgaaaacg tgcaggtatt cagccgattt
     4381 ttcaagagac tctcaggtat ttctcctacc gaatttcgac tcaaataaaa gaagagaatt
     4441 taggcggatt aggcttaaaa acatagcaat taaaaacgta gacctcttta agggcagatc
     4501 ttgaacagta gggtaaatcg atcttaataa gtttcgattt aattattgtg cgacctgatt
     4561 gactgagttt ttaatgtctt gaaaaaaact ctaactcagt aaggctgtca ttatggatat
     4621 aaaacagcac catagcttta tgctcagtgt tcctggtaca tggataggtc aaactaccag
     4681 cctttgctta agccatatat ctaaagcatt ctgtatctct ctgtttgggt acatattcta
     4741 tatacacctt gccatgtctt atactaaagc agggctcata ctattgggat tacggtaatg
     4801 cgcaattgta ggnnnnnnnn nntaggagca cacaaatgag caacctccgc aataactttg
     4861 taagtcgaaa taaagggtaa agagccaatg tactctaccc tttatttttt atatttgcaa
     4921 tattgattta caaggtaatc aaatgaaaaa gaatgtaata agcaccatta ctaaatcgtt
     4981 accgatagag ttttcctctc ttgtaaacta ctctttatta gtcactttgt gctatattaa
     5041 tctttttcaa taaaaataac cctcagcttt gacaaaactg tgattgaagc agccaaggcc
     5101 tatgccgaag agcagggtat tagcttgtcc cggctcactg aatacctcta taaacaaatc
     5161 acccgcaaag aatcacaaag ccttgaaagc ctgcccattg ccgattgggt tttgcagata
     5221 gccgaaggtg ctgccgaata caaaaccaaa ccccgcagcc gcaaagccaa tcgcgctgaa
     5281 tattttgatt cccgaaaaaa atgatcaagc cttttttgga tgccaaccta ctggtagctg
     5341 ttttgaacaa ggaatacccg gcgtttaccg atgccgcccg cgtgctgagc ctcgccggcc
     5401 aaaaaggttt cgttttgtat acttcacctc tttgcctggc cgataaaaaa gtgggcgacc
     5461 tggaagacag gctggaatac tatgccgccg aaaaagccgg gtgcacgcac ctgctgacaa
     5521 acgacacggg cgactaccat tttgctcgac taccagtcta tacgcccgaa gcttttcttc
     5581 gggaaattat tgggcggaac gatttatttg cgtagttttg cacccccaat cataagttct
     5641 ttttctgaat tggatttggg agcttccggc gccggcaacg gaaaccggga gcgctatcac
     5701 caaaaaaaca ttttacaatg gcaaaagaaa tctctggttt cgtaaagctg cagtgtaaag
     5761 gcggtcaagc caaccctgca cctcccatcg gcccggcact gggttccaaa ggtgtcaaca
     5821 tcatggagtt ctgcaagcag ttcaatgcaa gaacccaaga caagatgggc aaagtactcc
     5881 ctgtactgat cactgtttat tctgacaagt cttttgattt catcatcaag accccaccgg
     5941 catcggtaca gttgctggaa gcggccaaat tgcagggtgg atcaaaagag ccaaaccgct
     6001 caaaagtggg caaagttacc tgggcacagg tggaagcaat tgcgaaggac aaaatgcctg
     6061 acctcaactg ctttaccgtt gaaagtgcta tgaaaatggt agccggtacc gcccgtagca
     6121 tgggtatcac cgttgatggt gtagctccct gggaaaatta atcattgacc accaaactat
     6181 ttgagcaatg gcaattacta aaaaaagaaa agcagtaaac agcaaagtgg atgctgctaa
     6241 aatatactca ctgaaagaag cttcttcact cgtgaaagaa gtgaatacaa ccaagttcga
     6301 cgcttctgtt gacctgcata tccgtttggg tgttgatccc aaaaaagctg accaggccgt
     6361 tcgtggaacg gtaaccctgc cccatggcac cggtaaaacc aaaaaagtat tggttctctg
     6421 cccggccgac aaagaaaacg atgcaaagaa tgcaggtgct gactttgttg gactggatga
     6481 attcatcacc aagatcgaag gtggatggac agatgtagac gtgatcatcg ctacacctag
     6541 tgtgatgccg aaaatcggta agctgggtaa agtgcttggt ccgcgtaacc tgatgcccaa
     6601 ccctaaaact ggtaccgtta ccaatgatgt ggcagcagca gtgaatgaag taaaaggcgg
     6661 taaaatcgct ttcaaagttg acaaagctgg tatcgttcat gcttctatcg gtcgcgtaag
     6721 cttcaatgcc gacaagattg cggagaacag ccaggaattg atcaacgccc tcatcaaact
     6781 gaagccggca actgccaaag gtacttacct gaaaggcgtg agcatggcca gcagcatgag
     6841 tcctggtatc accatcgaca ccaaactatt cattcactaa gccccaaatt ttttgttatg
     6901 acaaaagaac aaaaacagga agtgatcgaa ctgctgaaag gcaagttcag ccaatatagc
     6961 aatttctata tcaccgatac cgagagcctg tcagtagacc aggtgtcaaa acttcgccgt
     7021 cattgcttta acaagcaggt ggaaatgaaa gtggctaaaa acaccctgat caaaaaagcg
     7081 cttgagcaaa tcgacgcaga aaagtacagc ggtgtatatg ctgccctcaa caatgtaacc
     7141 gccctgctgt tcagcgaaaa cccgaaagac ccggctgtta tcctgacctc tttccgtgca
     7201 gatgagaagt cggagcgccc ggtattgaaa gcagccttta tcaatggcga catttatgta
     7261 ggcgacgatc aactgaaaag ccttaccaag atcaaaacca agaacgaact cattggtgag
     7321 gttattggtc tgttgcaatc tccggctaag cgcgtaatcg ctgccctgct ggaaaaagca
     7381 aacaaagaag gtggtgccga agccgctcct gcaacagcag cagcagccga gtaacccata
     7441 tataattccc ctgcctgagg ggtctattta gtatttttta aaaatagccg gcggagcaaa
     7501 caggctgtga agtcggtaaa aactaaacaa aatggcagac gtaaaatcat tggccgaaca
     7561 actggttggc cttactgtaa aagaagtaca ggaactggca gacgtattga aatctgagta
     7621 tggtattgaa cccgctgctg ctgcagtagt ggtagctgct gacggtggtg ctggtgccgc
     7681 cgccgttgaa gagaaaacag ctttcaacgt tatcctgaag agcggtggtg cttctaaact
     7741 gaacgtggtt aaaatcgtaa aagacctgac tggtcttggt ctgaaagaag ccaaagaact
     7801 ggttgacggc gctcccaaag cagtgaaaga aggtgtttct aaagctgaag ccgacgatat
     7861 cg
//
LOCUS       DAONUO010000059         7845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7043_length_7845_cov_3.418100, whole genome shotgun sequence.
ACCESSION   DAONUO010000059 DAONUO010000000
VERSION     DAONUO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7043_length_7845_cov_3.418100"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK768_03340"
     CDS             complement(<1..586)
                     /locus_tag="PK768_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQA60033.1"
                     /translation="MEKLEDIIQAIDFVLKHVHEGIVIADRNGKVIYVNEANERITGL
                     DNKKVLGKQVIDVVPTSSIMDVIYSGKEKLGVNTKVGDKYVYSNIVPIRKDGEVIGGI
                     SVFLDMTEVEKLTRELKEAKQKINFLSKQLSGFIGDKDFIVGKNPKMQKVLQISQKAA
                     GVISNVLITGESGTGKEVVARYIHKNSPRKNKPFV"
     gene            748..1773
                     /gene="splB"
                     /locus_tag="PK768_03345"
     CDS             748..1773
                     /gene="splB"
                     /locus_tag="PK768_03345"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HQA60034.1"
                     /translation="MDMFVPRRALFERDALNYPIGKHIYHKMHELGVETSFIGSHNRV
                     MGIPGKTAQEAYFEGKRTLVVGIRRTLDFQTCKPSAHYQLPLVTSCIGECEYCYLNTT
                     LGKKPYIRVYVNIDDILSRAKEYINNRKPETTIFEGSATSDPLPVEPYSGLLKESIEF
                     FGREEFGRFRFVTKFTNVDSLLNVEHKGHTAFRFSINSESVIRKFEHRTPALDERINA
                     SVKVASAGYPMGFIIGPIFYYPEWRKEYVQMLESLAQQLSGKLSTPPAFELITHRFTK
                     KAKKNILEVFPKTQLPLDEEERRFKYGQFGYGKYVYPKEQMAEMKSFFLEKIRESFPD
                     SKILYFV"
     gene            1909..2268
                     /locus_tag="PK768_03350"
     CDS             1909..2268
                     /locus_tag="PK768_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQA60035.1"
                     /translation="MFFFGIFGIQQKERTIKEFDNIICPDCGRLTRAELMESYTYFHF
                     FFIPLFKWNRQYYLRLRCCGSIYMVEADYVDELRHSEDVDFDRLRKMQMPSNVCPNCG
                     SYIHPNFAYCPYCGQKR"
     gene            complement(2265..3020)
                     /gene="dapB"
                     /locus_tag="PK768_03355"
     CDS             complement(2265..3020)
                     /gene="dapB"
                     /locus_tag="PK768_03355"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQA60036.1"
                     /translation="MIKVVVSGAKGKMGRAISRLIGHKMDMKVIAGYDIYYEESDIVI
                     YDDLNKIPDTPDVIIDFSHPTVVKKLTEYAVDKGVALVVGTTGLEKEHKEMLFNASKK
                     VPVFVSHNMCMGVFLLINLAKQASKVLQGYDIEIVEKHHNQKIDAPSGTAIMIADAIK
                     EVRKDADYVYSRADVRQKRDKNEIGIHSIRGGNLVGEHTVLLIGENETIEITHKLASR
                     EVLAAGAVDVIRFIAKQKPGYYTMKDLMNSSLN"
     gene            complement(3175..3828)
                     /locus_tag="PK768_03360"
     CDS             complement(3175..3828)
                     /locus_tag="PK768_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQA60037.1"
                     /translation="MKKTKRVPVSIYAYNKIKTAILNNELMPGEKLVEASLAETLNMS
                     RTPIREALRQLEQEGFVTYYPQKGSIVSHISTTLAQELYEVREVLEGLAVRLICERAD
                     DIELVILKNIVDEMGEVISNKDYSTHVSLQKKWTENIILLAKNKILQDKLTSLNEHLA
                     RLRKISLANPDQNLEAYDEIVLTLSAIMDRDPMRAEKLARQHVINAKKRYYQYINLL"
     gene            complement(3856..5403)
                     /gene="citF"
                     /locus_tag="PK768_03365"
     CDS             complement(3856..5403)
                     /gene="citF"
                     /locus_tag="PK768_03365"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HQA60038.1"
                     /translation="MIKNGVGREIPEHLEHIGNLIPYESPFSIIPEGNKAGRKLKRQL
                     PHKSKLVSSIKEAVRACGLKDGMTISFHHHLRNGDYIVNMVIDTIASLGIKDIKLAPS
                     SLNPCHAPLIEHIKNGVVTSIETSGIREPLGTAISQGILPKPLIIRSHGGRARAIETG
                     ELKIDVAFLGVPCCDEYGNANGFYGKSAFGSLGYGLVDAKYADKVVLITDNLVDYPCL
                     PASINQTQVDFVVKVDSIGDPKGIVSGAIRFTKNPRDILIAENAVKAIEASGYFKNGF
                     SYQTGAAGASLAVTQFLREKMIKQNIKGSFALGGITSQIVQLLEEGLFGGIFDTQCFD
                     LDAVRSIGENPKHFEIDAATYASPFNAGCIANYLDILMLGALEIDTDFNVNVISGSDG
                     IISQAAGGHPDAAAGAKMSVILAPLIRARIPIIVDRVTTVVTPGETVDVIVTDYGIAV
                     NPRRQDLIERFKDARLNLYTIEELKEIAEKITGKPDKIDFTDRIIGVVQYRDGSVIDV
                     LREVKDI"
     gene            complement(5405..>6250)
                     /gene="citE"
                     /locus_tag="PK768_03370"
     CDS             complement(5405..>6250)
                     /gene="citE"
                     /locus_tag="PK768_03370"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HQA60039.1"
                     /translation="NPANIMGAHLYGADSIMFDLEDSVSIKEKDSARFLVHNALKTLD
                     FGNTETVVRINGLDTPFGREDLEAIVRARPDVIRIPKTEKAQDVLEVESIISEIEEKA
                     GIEVGTTKLMAAIESPLGVINAFEIAKSSKRLIGIALGAEDFVTSMKTERSKDGLELL
                     VARSQVLMAARAANIYALDTVFSDINDDEGFIKEVKLIKQLGFDGKSVIHPRQVTLVH
                     QVYTPTKKEIDYSIRVLNAIDEAEKRSSGVIALDGKMIDAPIVERAYRVLELAKASGV
                     YKGDY"
     assembly_gap    6251..6260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6291..6581)
                     /gene="citD"
                     /locus_tag="PK768_03375"
     CDS             complement(6291..6581)
                     /gene="citD"
                     /locus_tag="PK768_03375"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HQA60040.1"
                     /translation="MEIKRIGRAGTVESNDINIVIEPSDKEDIEIELQSAVIKQFGEH
                     IKALIIEILRSKGIKSAYVKAVDKGAIDCVIKARTLTALYRAAESEEYEWEV"
     gene            complement(6596..7624)
                     /gene="citC"
                     /locus_tag="PK768_03380"
     CDS             complement(6596..7624)
                     /gene="citC"
                     /locus_tag="PK768_03380"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HQA60041.1"
                     /translation="MGDLALRTVNLNRERERKEVELFLHRFELILEKDVEYTLALYDG
                     EKLIGTGSVSGNVLKCIAIDPNYQGEGMTNKLLSNLMNYQYDRGRTRLFIFTKPVNKR
                     IFQDMGFTEITHINDTVSLLENDPKGLSSFLDELKAKRREGKVISSIVMNCNPFTRGH
                     QFLIEHVSKNSDVVHIFVVWEDRSIFPNEVRYRLVRAGTKHLPNVIIHQGKDYIISNS
                     TFPSYFIKEKNSIVKIHAQLDLKIFGEFIAPVLGINRRYVGEEPVDPVTNEYNTVMKS
                     LLPKYGIEVFEVKRLEFEGLVISASRVRQAIKNDNVESIKDMVPKSTYDFLVSDEAKP
                     IIDRIKNL"
     gene            complement(7639..>7845)
                     /locus_tag="PK768_03385"
     CDS             complement(7639..>7845)
                     /locus_tag="PK768_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HQA60042.1"
                     /translation="GQLLTNKYRMQMNESMLAATPAGATDMALISADIGIESADVIVL
                     QIIRMITVISLFPQIIRLIVFITK"
BASE COUNT         2123 a         1741 c         1365 g         2606 t
ORIGIN      
        1 ctacaaaggg tttgtttttt cgggggctgt ttttatggat gtatctggct accacttctt
       61 ttccggtccc actttcaccg gtgatgagca cattggaaat gactcctgca gctttttgcg
      121 aaatctgcaa aaccttttgc attttagggt ttttccccac aataaaatct ttatcaccta
      181 taaagccgga caactgctta gacaggaaat taattttttg cttggcttct ttcagctctc
      241 gagtgagttt ttccacctct gtcatatcta aaaatactga tatgccacct atgacctcac
      301 cgtctttgcg tattggcact atatttgaat acacatattt atcgccaact tttgtattga
      361 caccaagttt ttccttgccg gaatatataa catccattat tgacgaagta ggaaccacat
      421 ctattacttg ttttcccaag acctttttgt tatctaatcc cgtaatcctt tcattggctt
      481 cattcacata aatgactttt ccgttccgat cggcaatcac tatcccttcg tgaacgtgtt
      541 ttaacacaaa atctatcgct tgaataatat cttcaagctt ttccatgcta tccccccaca
      601 actcgtaaat cggagctcct gtaatgtttt acttcgtcta tggatgtaaa aaatcctttt
      661 agtgtgacaa aaaactttct attttcggat gatgcttcgg gttttaaact cgctaaatcg
      721 gataaactaa ttcatggtga tgatttcatg gatatgttcg taccaagaag ggctctgttt
      781 gaaagagatg cactaaatta tcccatagga aagcacatct accacaaaat gcacgaacta
      841 ggtgttgaaa catcttttat aggctcgcac aatcgtgtaa tgggaatacc cggcaaaacc
      901 gcccaggaag cctactttga agggaaaagg accctggttg tggggataag aaggaccctg
      961 gattttcaaa cctgtaagcc ttcagcccat taccaattgc cactggtaac tagctgtatc
     1021 ggggaatgcg agtactgtta tcttaatacc acattgggca agaaacctta cattagggta
     1081 tatgtaaata ttgacgacat cctgtctagg gcaaaagagt atataaataa cagaaagccg
     1141 gagacaacta tttttgaagg ctctgctaca tccgaccctc tacccgtgga accttacagc
     1201 ggtttattaa aagaatccat agagtttttc ggtagggaag aattcggccg ctttagattt
     1261 gtaaccaagt ttacaaatgt tgattccctc cttaatgtag agcataaggg tcatacggcc
     1321 tttagattca gcattaactc ggaaagtgta atacgaaaat ttgaacaccg aacgccagct
     1381 ttagacgaaa ggattaatgc atcggtaaaa gtagcatctg ccggctatcc aatgggtttt
     1441 ataataggcc ctattttcta ttatccagag tggagaaaag agtacgtaca aatgcttgag
     1501 agcctagctc aacagctttc cggtaaatta agcactccac cggcctttga actaatcacc
     1561 catcgcttta ccaaaaaagc aaagaaaaat attttagaag ttttccccaa aacacaactt
     1621 cctcttgatg aagaggaaag aagatttaaa tacggtcaat ttggatatgg aaaatatgtt
     1681 taccctaagg aacagatggc tgaaatgaaa tctttcttcc tagaaaaaat ccgggaaagt
     1741 ttcccggact ccaagatttt atattttgta tagcttaatg gtgcctatcg atagctttcc
     1801 tgctacgtaa aagccccaaa actatagaac agctttgggg ctttatttaa ctatttaatt
     1861 gttgtataat ttacattaat gatctagctt tctttagggg gaatttttgt gtttttcttt
     1921 ggcatctttg gaattcagca aaaagaacgc acaataaagg agtttgacaa cattatctgt
     1981 cccgattgtg gaaggctgac tagggcggag ctaatggaat catatacata ttttcacttc
     2041 tttttcattc cgctttttaa atggaacaga caatattatc ttcggttaag atgctgcgga
     2101 agcatttata tggtagaagc tgactatgta gacgaactta ggcacagtga ggatgtcgat
     2161 tttgaccggc tgcgtaaaat gcaaatgcca agtaatgttt gtcccaactg cggatcttat
     2221 atacacccca attttgcata ttgcccctat tgcggtcaaa aacgctaatt tagactagaa
     2281 ttcatcagat ccttcatggt gtaatagccc ggtttttgtt tggcaataaa cctgatgaca
     2341 tcgactgcac cggcagctaa aacctccctt gatgccagtt tatgggttat ttctattgtt
     2401 tcattttcac ctattaataa aaccgtgtgc tcacctacca agttgccccc tcgaatagaa
     2461 tgaatgccga tttcattttt atcccgtttt tgcctgacat cggccctgct atatacataa
     2521 tcagcatctt ttctaacctc tttaattgca tctgcaatca ttatagctgt tccgctggga
     2581 gcatctatct tttgattgtg atgtttttct acaatctcaa tatcatagcc ttggagtact
     2641 ttagatgcct gttttgccag gtttattagt aaaaatactc ccatgcacat attgtgcgaa
     2701 acaaagacgg gaaccttctt tgaagcatta aaaagcattt ctttgtgctc tttttccagg
     2761 ccggtagtgc cgacgactaa tgcaactcct ttatccacag catattcggt gagctttttt
     2821 acaacagtgg gatgggaaaa gtctataatc acatcgggag tatccggaat tttattcagg
     2881 tcatcatata tgactatatc gctttcttca taatagatat cataacctgc aataactttc
     2941 atatccattt tatgacctat cagccgagat atggctctgc ccattttacc ttttgcaccg
     3001 cttactacta cttttatcat gtaaatccct ccagtattgc aataatgatt gctataaatt
     3061 taatatgtat attaccattt tgattagcgc tcttcaagca cctagttttc cgtaagtgat
     3121 gcaaaattca ttttgacatt ccaaaacaaa cgctccttct aggagcattt gtttttacag
     3181 cagatttata tactggtaat atcttttctt agcatttatc acgtgttgtc ttgctaattt
     3241 ttctgctctc attggatctc tgtccataat ggccgataag gtcaatacta tctcatcata
     3301 cgcttctaag ttttggtcgg gattggcaag ggatattttc ctcaatcttg caagatgttc
     3361 atttaaagaa gtcaatttat cttgtagtat tttgttttta gcgaggagaa taatgttttc
     3421 cgtccacttc ttttggagag atacgtgagt gctgtaatcc ttatttgata tgacttctcc
     3481 catttcatct acaatattct ttagtataac cagttctata tcatccgccc tttcgcaaat
     3541 aagcctaact gctaatcctt ccagcacttc tcttacttcg tatagttctt gagctaaagt
     3601 agttgatata tgactaacga tcgaaccctt ttgcggataa taagttacga aaccctcctg
     3661 ttccaattga cgaagagcct cccttatagg agttctactc atattaaggg tctccgctaa
     3721 ggatgcttca actaattttt ccccgggcat tagttcgttg ttcagtattg cagttttaat
     3781 tttgttgtaa gcatatatgc ttaccggaac tctcttcgtc tttttcattt tatcacaaac
     3841 ctttttgata tttctttata tatctttcac ttctcttaac acatcaataa cagaaccgtc
     3901 tctgtactgt actactccaa taatcctatc tgtaaaatca attttgtcag gcttgcccgt
     3961 aattttttca gcaatttcct tcagttcttc aatagtgtaa agattcaacc ttgcatcttt
     4021 aaaccgctct atcaaatcct gtcttcttgg atttactgca ataccatagt cagttacaat
     4081 tacatcaacg gtttcacccg gagtgacgac ggtggtgacc ctgtcaacaa taatcggtat
     4141 tctcgctcta attagtggcg ctaagatcac ggacattttt gcgcccgctg ctgcatcagg
     4201 atggcctcct gccgcttgac taataatacc atcagagcct gaaatcacgt tgacattgaa
     4261 atccgtatct atttctaaag cgccaagcat cagtatatcc agataattgg caatacaacc
     4321 cgcattaaag gggctggcgt aagtagccgc atctatctca aagtgttttg ggttttcccc
     4381 tattgacctt accgcatcta aatcaaaaca ctgcgtgtca aatatacctc caaacagtcc
     4441 ttcttctaat aattgaacta tttgcgaagt tatgccgcct aaggcaaaac tgcccttaat
     4501 gttttgttta atcatctttt ctcttaagaa ttgggttact gccaaggatg ctcctgctgc
     4561 tccggtctga taagagaacc cgtttttaaa gtacccagag gcttcaatgg cttttacggc
     4621 attttcagct atcaagatat ctctagggtt ttttgtaaac cttattgcac ctgaaactat
     4681 gcctttagga tcgccgatag aatcaacttt aacgacaaag tcgacctggg tctgatttat
     4741 gctggctggc aaacacgggt agtcaaccaa gttatcggtg atcaaaacta ccttatcggc
     4801 atattttgca tctaccaaac catatcccag ggaaccgaag gccgattttc cgtaaaaacc
     4861 gtttgcattg ccgtattcgt cgcaacaagg cactcctaaa aaggcaacat ctatttttaa
     4921 ttcgccggtt tctatagctc tagctctgcc gccgtgggat cttatgatta atggtttagg
     4981 taaaataccc tgcgaaattg ccgttccaag gggttcccgt ataccgctcg tttcaatgct
     5041 tgtcactact ccattcttta tatgctctat taaaggtgcg tggcaaggat taagggaact
     5101 tggagccaat ttaatgtcct ttatgcccaa ggatgcgatt gtatcaataa ccatattcac
     5161 tatgtagtca ccgtttctta aatgatggtg gaatgatatg gtcatcccat cttttaggcc
     5221 gcaagctctc actgcttctt taatagaact cacaagttta cttttatggg gcagttgtct
     5281 ctttagtttt cgccctgctt tattcccctc gggaatgatg ctaaaagggc tttcgtaagg
     5341 tatgaggttt ccgatgtgtt ccaggtgttc aggtatttcc ctgcctactc cattctttat
     5401 catattaata gtccccctta tatactccag atgctttggc taactctaaa accctgtatg
     5461 ctctctcaac aattggagca tcgatcattt taccatccaa ggctatgacg ccagaagatc
     5521 tcttttcagc ttcatctatg gcatttaata cgcgtatgga ataatcgatt tccttcttag
     5581 ttggagtata gacctgatgc acaagtgtca cctgcctcgg atgtattacg gatttgccgt
     5641 caaagcccag ctgtttaatg agctttactt ctttaataaa accttcgtca tcgtttatat
     5701 ccgaaaatac cgtatctaag gcatagatat tagcagccct agctgccatc agtacttggc
     5761 tccttgccac caacagctca aggccatctt tagacctttc ggtcttcatg cttgtaacga
     5821 aatcttcagc acctaaggca attcctatca accttttgct ggatttagct atttcaaagg
     5881 catttattac acccaagggg ctttcaatgg ctgccatcag ttttgtggtt ccgacttcaa
     5941 tgccggcttt ttcttctatt tccgatataa tggattccac ctcaaggacg tcctgggctt
     6001 tttcagtttt aggtattctt ataacatcgg gtcttgccct aacaatggct tccaaatctt
     6061 ctcttccgaa aggtgtgtca aggccgttta ttctgactac cgtctcagta tttccaaaat
     6121 caagtgtctt taatgcgtta tgcaccagga accttgccga gtctttctcc tttatggata
     6181 ctgagtcttc caaatcaaac attatagaat cagctccgta tagatgggct cccataatat
     6241 ttgcgggatt nnnnnnnnnn atcatggttc ttctcagcct taccatgcca tcacacctcc
     6301 cactcatatt cttcagactc agctgctcta taaagagccg tcagtgttct tgccttgata
     6361 acgcaatcta ttgcgccctt gtctacggct tttacgtaag cactttttat ccctttgctc
     6421 cgcaatattt ctataataag ggctttaatg tgttccccga attgctttat tactgcactc
     6481 tgcaattcga tttctatatc ttctttatcc gacggttcaa taacaatatt gatatcattt
     6541 gattcaacgg tgcctgccct gccgatcctt ttgatttcca aatgctatac ctcccctaga
     6601 gattttttat tctgtcaata atcggcttag cctcatcgga taccaaaaaa tcatatgtgc
     6661 tttttggaac catatcctta atcgattcaa cattatcatt ttttatcgcc tgcctgactc
     6721 tcgaagcact aatgaccaag ccttcaaact ctaatctttt tacttcgaat acttcgatgc
     6781 cgtatttggg cagaagactt ttcatcaccg tattgtattc attggtaaca gggtcaaccg
     6841 gttcttcgcc tacatacctc ctgttgatac ctaagacagg tgcaatgaat tctccaaaaa
     6901 tcttcagatc caattgagca tgtatcttta caattgagtt tttttctttg ataaaataag
     6961 aagggaatgt tgagttggaa ataatataat ctttaccctg gtgaattatc acattaggta
     7021 agtgttttgt tccggctctc accaacctgt atctaacttc attcggaaat attgacctat
     7081 cttcccaaac gacaaagata tgaactacat cactattctt agaaacatgt tcaatcaaaa
     7141 actgatgtcc ccgtgtaaaa gggttgcagt tcatgacaat acttgatatt accttgcctt
     7201 ctcgcctctt agcctttaat tcatctagaa aactgcttaa acctttcgga tcgttttcaa
     7261 gcaaggaaac tgtatcgttt atatgggtaa tttcggtaaa gcccatatcc tggaatattc
     7321 ttttgttcac cggtttggta aatataaaca aacgagtgcg gcccctatcg tattgatagt
     7381 tcatcagatt tgagagaagt ttattagtca taccctcccc ttggtagttg gggtcaatag
     7441 ctatgcactt tagcacattg cccgatacgg agccggttcc tattaatttc tcaccatcgt
     7501 aaagtgctaa ggtatactct acatcctttt caagaattaa ttcaaatcta tgtaaaaaca
     7561 gttctacttc cttcctttcc ctttctctat tcaagtttac ggttctcaag gctaaatctc
     7621 ccatgatctt ctcttccttt atttagtaat gaatacaatc aatcgtataa tttggggaaa
     7681 cagcgagatg acagtaatca tgcggataat ttgtagaact attacgtccg cactctctat
     7741 ccctatatca gccgaaatca gtgccatatc cgttgcacca gccggtgttg ctgccaacat
     7801 ggattcattc atttgcatgc gatatttatt agttagcagc tgacc
//
LOCUS       DAONUO010000060         7827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7057_length_7827_cov_3.633042, whole genome shotgun sequence.
ACCESSION   DAONUO010000060 DAONUO010000000
VERSION     DAONUO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7057_length_7827_cov_3.633042"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PK768_03390"
     CDS             complement(<1..467)
                     /locus_tag="PK768_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HQA60043.1"
                     /translation="MNFTRDEIIETIRMVQAEQLDIRTITLGISLRDCAGSSISQVAD
                     KIYDKLMEKGSELVPVANQLQEEFGIPIVNKRISVTPIAMVAESCEETDYTPIALAMD
                     KSAKDLGIDFIGGFSALVQKGFTRGDMNLLNSIPDALSRTERVCSSVNVATSKS"
     gene            complement(479..928)
                     /locus_tag="PK768_03395"
     CDS             complement(479..928)
                     /locus_tag="PK768_03395"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQA60044.1"
                     /translation="MDILVTLDVLKQHRDKKEVNFPPNTLVTKEARIYAQNHGIELYI
                     GDKKVDEPVQSSSSPIVRAVITVIGEDRVGIIAGISDVLANANVNILDITQTAIEGLF
                     TMIMVVDIAKSTLSFDELTKQLSRRGEELSVRVDAQREEVFHFMHRI"
     gene            1152..1631
                     /locus_tag="PK768_03400"
     CDS             1152..1631
                     /locus_tag="PK768_03400"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HQA60045.1"
                     /translation="MVMLPLVISIIFFILGLLGTVLPVLPGAILIYAGMLVYGIMTGF
                     KSMTVNFFLIQALILIIIFLVDFLASLIGTRHFGGSKQAIYGAGIGTLLGLFIMPPVG
                     IFVGPFLGAVIAELLLGKQLQEAIRVGIGSFIGIVGGTLLKLLMEIIMIVYFFIQVY"
     gene            complement(1742..3094)
                     /locus_tag="PK768_03405"
     CDS             complement(1742..3094)
                     /locus_tag="PK768_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQA60046.1"
                     /translation="MDLARSQLGEKIVGQGLKYLLSKDIDDLDKLIYWAEKLPMPQNH
                     RVYLESAKKFLKDKDSNWYQFAQRLLRETNPYVKEKIGMNFFLNANFLGVQRQNKMAK
                     ELGHSVPWAILIDPTERCNLNCTGCWAGDYQRSEELDFETLDRVMTEAEELGIFFTIM
                     SGGEPTLRKRDIIKLAEKHPSQVIHLFTNGTLVDDEFVAEMMRVGNITLAFSIEGFEE
                     STDSRRGKSVFKKVMEAMDRMRKAGLIFGVSVTYTRKNTEELASEEFVDMLVDKGATF
                     AWYFTYVPVGKDVDVELMATPEQRAYMYEKVLEYRRTKPIFIMDFWNDGEISHGCIAG
                     GKRYLHINAKGDVEPCAFVHYATCNIKDLSLKDALSSPLMLAYQKRQPFNMNHHRPCP
                     LIDNPEMMVEIVRESGAYSTQVNADETPEEFAQKLKNYSDSWGRLADAKWAKSCAAVK
                     "
     gene            3251..3517
                     /locus_tag="PK768_03410"
     CDS             3251..3517
                     /locus_tag="PK768_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60047.1"
                     /translation="MTKIYRDTIVRMYIMREVIKMLVDNIIHNISFSSLAVFILSGIY
                     LLKIDRIDLSIKGLRVEEKAATIIGILYIFGSIAVFVLFRFFIM"
     gene            3675..5282
                     /locus_tag="PK768_03415"
     CDS             3675..5282
                     /locus_tag="PK768_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HQA60048.1"
                     /translation="MTNIFSKLYSNKKQELFFERDMSPDKSRKLKTDIAENLNIVNEY
                     LYDCDDVITRIFYMGHTAKHKMALVYIDGMVAMDTVQNEVLKPLMLFACQFPLEYTVG
                     EKFFEMVKEGLLTAGDIKETDNFGDVILGILSGDTILFMDKLEKALVISTRGWEKRGV
                     EEPSTEAVIRGPRDGFTETYRTNVALVRRRLKDPNLKVKTIKLGRHTRTDVGIMYIKG
                     VAKPSIVDEVLKRLNTIDISCVLESGYIEQMIEGDWLSPFPQLRRTERPDVVAASLVE
                     GNVAILCDNTPFALLAPTTFLSLFQSPEDYYERWYIVSMIRILRFIAGFFAFTFPSLY
                     IAMTTFHPGMLPTDLVLSIAAAREGVPFSAVLETLIMIVSLEVLREAGVRLPGPIGQT
                     IGIVGGLVVGEAAVRASIVSPIMVIVVAINAISSFAIPNYSVAISFRLLTFIMIALAS
                     IAGLYGIMLGLLGLTIHLVTLKSFGAHYLSPFVSFRFSESKDNIIRGPLPSMEIRPGY
                     TRPQDFDRINDRRHDTIDKAGDERADE"
     gene            5269..6366
                     /locus_tag="PK768_03420"
     CDS             5269..6366
                     /locus_tag="PK768_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HQA60049.1"
                     /translation="MLTNNDMITVPQAVGLMIMTILEIGLLILPREVAIYAGSDGWIL
                     VIVGGLLALAGSLVLSTLIRRFPGETFIEYSGKVIGKFLSFVVGVGLVIHFTLATSIV
                     IRTFTDVTNAFMLENTPREFIIITQMLLTVYLIRHGIEPTARIAEILFPILIVPIFAM
                     YLIAIPKADFTELLPVFNTPIKSMAMGSLNTMFSFFGVEILLMLGPYLKRPDKIYWTM
                     FVSVGVATLIYLFVVVITFVGIGVQNTKMLIWPGMTIIRMITAPGRVFERLDALAMAL
                     WTIASFTTANSLYLTGALTAFHLTKAREFKFFISILFPWIYFIASLPPNIIATEKLVY
                     VVKWGSIPVGIIIPFFILLLAVILKKKGGTA"
     gene            6363..7508
                     /locus_tag="PK768_03425"
     CDS             6363..7508
                     /locus_tag="PK768_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HQA60050.1"
                     /translation="MNRYKKLLICILSSMLLLVGCWDMVEIDRRLFVGIVGIDTSDEP
                     NKYTFYFSIPIAREIIGGEGGGGGGGGESVGLLTTEAYSLVDAARNLALRINRDIYFE
                     HMRIVVIGEDMARKHLKAILNPFARQPEFNRRSRIAICEGRAEKVMGVTPWTEKLKAE
                     YMDSLYAARAMSGKFIEMDLGDFFRNLNSQNGNILVSKITPNKTEVNIGGAGVIKDFK
                     LIGWLNEEETQGVNFFLGKVRGGDIVVRDIRGEGTETFTILGEKTKLSLKAAEPVPEF
                     LLEVYVTGNIASTAHYITYYRSKLESKDIERMEELISKEIESQILKGVKKLQEDYKVD
                     LLKLGNFLQKHEPALWKTYEDNWEEIFTQVNIDINVSTTIKNIGILR"
     gene            7584..>7827
                     /locus_tag="PK768_03430"
     CDS             7584..>7827
                     /locus_tag="PK768_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HQA60051.1"
                     /translation="MKHKSKLLKKTFTVTLGILVLSMLCAIVSSSGLAGGIDGSTGVT
                     ANQLADKLIRLHVIANSNSPEDQELKLKVRDAINSAL"
BASE COUNT         2125 a         1690 c         1806 g         2206 t
ORIGIN      
        1 gacttgctgg tggccacatt taccgacgag cacaccctct ccgtccggga cagggcatcg
       61 ggaatggaat taagcaaatt catgtcaccg cgggtaaagc ccttctgcac cagtgcggaa
      121 aagcccccta taaaatcaat gcccaaatct tttgcggact tgtccatggc cagggctatc
      181 ggggtgtagt ccgtctcttc gcagctttcg gccaccatgg caatgggagt caccgaaatc
      241 ctcttgttca caatgggaat gccgaattcc tcctgcaact ggttggccac cgggaccagc
      301 tcgctgcctt tttccattag cttatcgtat attttatcgg caacttggga aattgaggag
      361 ccggcgcaat ccctgaggct tattcccagt gtaattgtcc tgatatccag ctgttcagct
      421 tggaccatgc gtattgtctc tattatctca tccctggtaa agttcatcta aaatccccct
      481 aaatcctgtg catgaaatga aatacttctt cccgctgggc atcaactcga accgaaagtt
      541 cttcgccgcg gcgtgaaagc tgtttcgtca actcatcaaa actcaaagta gacttggcta
      601 tatccactac catgatcatg gtaaacaggc cttcgatggc cgtctgggta atatctaata
      661 tattgacatt ggcgtttgcc agcacatcag aaatgccggc aatgattcct accctgtcct
      721 caccgataac ggtgatgaca gccctaacga tgggactgct gctggattga acgggttcgt
      781 ccaccttttt gtcccctatg taaagctcta tgccgtggtt ttgggcatat attcgggctt
      841 cctttgtcac aagggtattt ggtggaaaat taacttcttt tttgtccctg tgctgtttta
      901 atacatcgag ggttactaaa atatccataa aatgcaaccg attagcttga aataaccggc
      961 ttcctacacc tgcctttcaa ttttcaatat tatgtaatta gtataaatca aaagaatcgg
     1021 agtatcaaga gcggcagtga actcggctaa ttgataaatt agcggtgtcc atgtgtaacg
     1081 tcaacaaaaa atatttactg ccttgtcagg cggtgataaa ataattatag cttagcaaaa
     1141 ggaggcataa tatggtgatg ttacctttgg tcatatccat tatatttttc atcctgggtt
     1201 tactgggaac ggtattgccg gttttaccag gcgccatact gatatatgcc ggaatgcttg
     1261 tctacgggat aatgaccggc tttaaaagca tgactgtgaa tttttttctt atacaggcct
     1321 tgatcctgat aataatcttt ttagtcgatt ttttagcatc tctaattgga acccgccact
     1381 ttggcgggag taagcaggct atatacggag caggaatcgg cacactgctg ggtttattca
     1441 taatgcctcc tgtaggcatt tttgtgggtc ccttcctggg tgccgtaatt gcggaactgc
     1501 tgctcggcaa gcagctacaa gaagctatcc gcgtggggat aggaagcttc atcggcatag
     1561 taggcggaac cttattgaaa ttactcatgg aaatcatcat gattgtctac tttttcattc
     1621 aagtctatta aacacagctc actaaagctt gccttttcgt agccaaaaat tgcccaaagc
     1681 aaatgacgcc aattatcttt ggcgtcattt gggttttaaa tatatccctt tctaataata
     1741 ctcattttac tgcagcgcag gactttgccc acttggcatc ggctaaacgg ccccagctat
     1801 cggaatagtt cttgagtttt tgggcaaatt cttcgggagt ctcatcggca tttacttgtg
     1861 tggaatacgc cccgctttcc cttactattt ccaccatcat ttcgggatta tcgattagag
     1921 gacatggcct gtgatgattc atgttaaagg gctgtcgttt ttgataggcc agcattaagg
     1981 gcgaactcaa agcgtctttt aatgataggt cttttatgtt acatgtggca tagtggacga
     2041 aggcacaggg ttccacatcg cccttggcgt taatatgtag gtaccgcttt cctccggcta
     2101 tacagccatg ggaaatctca ccgtcattcc aaaagtccat aatgaatatg ggcttagtgc
     2161 gcctgtactc caggactttt tcgtacatgt aggcgcgctg ctcgggagtt gccatcaatt
     2221 ccacatccac gtcttttcca actgggacat aggtgaagta ccaggcaaaa gttgctccct
     2281 tgtcaaccag catatccaca aattcctcac tggccaattc ttccgtgttt ttccgcgtgt
     2341 acgttacgga aactccgaag ataaggccgg ctttgcgcat gcgatccatg gcttccatta
     2401 cctttttaaa cacactcttg ccacgccttg aatcggtgct ttcttcaaag ccttcaatgc
     2461 tgaaggccaa ggtgatattg cccactcgca tcatttcagc cacaaactca tcgtccacca
     2521 aagtgccgtt ggtaaacaga tgaatcacct gggatggatg tttttcagca agttttataa
     2581 tgtctctttt ccgaagagta ggttcaccgc cggacatgat ggtaaagaat attcccagct
     2641 cttcagcttc agtcataacc ctgtctaagg tttcaaagtc cagttcctcg cttcgctggt
     2701 aatctcctgc ccagcagccg gtgcagttta gattacatct ttcggtggga tcgattaaaa
     2761 tagcccacgg aacagaatgc cccagctctt ttgccatttt attttgtctt tgaacaccta
     2821 aaaagttggc atttaggaaa aagttcatgc cgattttttc ttttacgtag gggttcgttt
     2881 ccctaaggag cctttgggca aactgatacc agttggaatc tttgtctttc aaaaacttct
     2941 ttgcgctttc aagatacacc ctgtggtttt gaggcatggg caacttttcc gcccaataga
     3001 tcagcttatc aagatcatct atgtcttttg acagtaaata tttcagcccc tggccaacaa
     3061 tcttctctcc aagttgcgac ctggccaaat ccatacataa agacctcctt acgattcgtt
     3121 gtatcgatat ataacgatac atctacttat atcatattcc catggaaggc ttttgtcaag
     3181 aaccgggttt aggtcggcgg aaaaagcgtt gtaaattgga aagcgagctt attcataaaa
     3241 agagccgata atgacaaaaa tatatagaga taccattgta aggatgtata taatgcggga
     3301 agtaataaaa atgctagtag acaacattat ccacaacata tctttttcaa gccttgctgt
     3361 attcatttta agcggcattt accttttgaa aatagatagg attgaccttt ccataaaggg
     3421 cctgagagtt gaggaaaaag ccgctacaat tattggcatc ttgtatatat tcgggtcaat
     3481 tgcagttttt gtgctgttca ggttttttat tatgtagcta cttttcaaga aaatgcgttg
     3541 tagtgaatgt agtagtcgct gatactcggt ggtaatatgt ttcagtaact ttagttgtca
     3601 ggtgataccg ttcgctcaag tttatttttt cgatggttct aaatacataa tttgagcaaa
     3661 ataggagtgc ccgcatgacc aatatatttt ccaaactcta cagcaacaaa aaacaggagc
     3721 tattcttcga aagggacatg tcccccgata agagccgaaa acttaaaacc gatatcgcag
     3781 aaaatctcaa cattgtcaac gagtatctct atgactgcga tgatgttatt acccggattt
     3841 tttacatggg ccatactgct aagcacaaga tggcccttgt gtacatcgat ggtatggttg
     3901 ccatggatac ggtgcaaaat gaagtattaa agccattgat gctgtttgcg tgccaattcc
     3961 ccttggaata cactgtgggt gaaaagtttt tcgaaatggt aaaagaaggt ctcctgacgg
     4021 caggagacat aaaagaaacg gataacttcg gcgacgtcat actgggcatt ttatcgggtg
     4081 acaccattct gtttatggat aaactggaaa aggccttggt aattagcacc aggggatggg
     4141 aaaagcgagg agttgaagag ccatctacgg aagcggtgat tcgagggccc agggatggtt
     4201 ttaccgaaac gtaccgaaca aatgtagccc tagttcgtcg gaggttgaaa gatccgaacc
     4261 tgaaagttaa gacaattaag cttggcagac atacgagaac tgatgtaggc atcatgtata
     4321 ttaaaggcgt agccaagccc tctattgtcg atgaagtatt aaaaaggttg aataccatag
     4381 acataagctg tgtgctggaa agcggatata tagagcagat gatagagggg gactggcttt
     4441 caccatttcc gcagcttcgg cgcacggagc ggcccgatgt ggtggctgca tcactggtag
     4501 aaggaaatgt agccatctta tgtgacaata ccccctttgc tctccttgca cccacaacct
     4561 ttttgagcct gtttcagtcg cctgaagatt attatgagcg ctggtacatc gtttcgatga
     4621 ttaggatttt gcgatttata gcaggtttct ttgcctttac ctttccatcc ctgtacattg
     4681 ccatgactac ttttcatcct ggcatgcttc cgacggacct ggtcctgtct attgcggctg
     4741 ccagggaagg tgtgcccttt tcggcggtgc tggaaaccct catcatgatt gtttcgctgg
     4801 aagtcctaag agaggcagga gtgagacttc ccggccctat aggtcagaca ataggtatag
     4861 tgggcggttt agtagtaggt gaagctgcag tcagagccag cattgtaagt cctatcatgg
     4921 tcattgttgt ggcgataaat gccatttcgt cctttgccat ccccaactac agcgttgcta
     4981 taagctttcg cctgctgacc tttataatga tagcactggc cagtattgca ggactgtacg
     5041 ggattatgct gggccttttg ggcctcacaa tacaccttgt tacgttaaaa agctttggag
     5101 cccattacct gtcacctttt gtttctttca gattttccga aagtaaggac aacattatcc
     5161 gagggccttt gccctccatg gaaataaggc ctgggtatac gaggcctcaa gactttgaca
     5221 ggataaatga caggcgacat gataccatcg ataaagcggg ggatgaacgt gctgacgaat
     5281 aatgatatga taaccgttcc ccaggccgtt ggcttaatga taatgaccat tttggaaata
     5341 ggcctcctaa tcctcccgag ggaagtggcc atatatgccg gttcggacgg ctggatcctg
     5401 gtgatcgtag gagggctttt ggctcttgcc ggcagcctgg tcctgagcac tcttataagg
     5461 agatttccgg gagagacctt catagagtat tccggaaaag tgataggcaa attcttgtcc
     5521 ttcgtagttg gagttggtct agttatacat tttaccctgg ccacttccat agttatcagg
     5581 acctttaccg atgtcaccaa cgcattcatg cttgaaaaca ctcccaggga gtttataata
     5641 attacccaaa tgctcctcac ggtttacctg atacgccacg gcattgagcc gacggcgaga
     5701 attgcggaaa tactcttccc tatactgata gtgcctatct ttgctatgta cctgattgcc
     5761 ataccaaaag cagattttac tgaactcctg ccggttttta acacacccat aaaatcaatg
     5821 gcaatgggaa gcttaaatac catgttcagt ttttttggag ttgagatttt gctaatgttg
     5881 ggtccctatc taaagaggcc tgacaaaata tactggacca tgtttgtaag cgtgggagtt
     5941 gctacgctga tatatttatt tgtcgtggtt attacttttg tgggaattgg tgtacagaac
     6001 accaagatgc taatatggcc gggcatgacc attatccgca tgataacggc gccgggaaga
     6061 gtctttgaga ggttggatgc cctggccatg gccttatgga ccattgcctc ctttaccacg
     6121 gccaacagcc tgtacctaac gggagccctg actgcctttc accttacgaa ggccagagaa
     6181 ttcaagttct ttataagcat tttgtttcct tggatttatt tcattgcttc ccttcccccg
     6241 aatatcattg caacggaaaa gttagtgtac gtggttaaat gggggagcat tcccgtaggc
     6301 atcatcatac cctttttcat tttactactg gcagtcatct tgaaaaagaa gggcgggaca
     6361 gcatgaatag gtacaaaaag cttttgatat gtattttatc gtccatgctg ttattggtgg
     6421 gatgttggga catggtggaa atagacaggc gactttttgt gggaattgta ggcatagaca
     6481 caagcgatga gcccaacaag tacacctttt acttttccat acccatagct agggaaatca
     6541 taggcggcga aggcggcgga ggaggtggag gcggagagag tgtgggcctt ttgacgacgg
     6601 aagcctattc attagtagat gcggcgagga atcttgcctt acggataaac cgggacattt
     6661 actttgagca tatgaggata gtagtaatcg gagaagatat ggcaaggaag cacttaaagg
     6721 caatcctgaa tccttttgcg cgtcagccgg aatttaacag gcgaagtcgc atagccatct
     6781 gcgagggcag ggcagaaaag gtcatggggg ttactccctg gacggaaaaa ctcaaggcag
     6841 aatatatgga ttccctatat gcagccaggg ccatgtcggg gaaattcatc gaaatggatt
     6901 tgggagattt ttttcgaaat ctcaattccc aaaatggaaa tatactagtt agcaaaatta
     6961 ctccgaacaa aaccgaggta aatattggcg gtgcgggagt aataaaggac tttaagctta
     7021 taggctggct caatgaagag gagacccagg gcgttaattt ctttctcggc aaggtaaggg
     7081 gcggggatat agtggtaagg gacatacggg gtgaaggaac ggaaactttt acgatacttg
     7141 gtgaaaagac aaagctttcc ctcaaggcgg cagagcccgt gcccgagttt ttattggaag
     7201 tgtacgtaac cggcaatatt gccagcaccg cccactatat cacatattac cgttcgaaat
     7261 tagaatcaaa agatattgag cgaatggaag aactcatatc aaaggaaatt gaatctcaaa
     7321 tcctaaaagg ggtaaaaaag cttcaggagg actacaaagt ggatttactc aagctgggga
     7381 attttttgca aaaacatgag cccgctctgt ggaaaacata tgaagacaat tgggaagaaa
     7441 tattcaccca agtgaacatc gacattaatg tcagcacaac cataaaaaat ataggtatct
     7501 tgcgataaaa agcattttaa atgattataa gggctgattt cgggaataat aataacaaaa
     7561 catttgcggg ggagagagag caagtgaaac acaagtcaaa actcctgaaa aagaccttca
     7621 cggtaacact gggaatactg gtactttcaa tgctctgtgc gatcgtcagc agcagcggtc
     7681 ttgctggagg catcgatggc agcaccggag tcacggcaaa tcagctggca gataaactta
     7741 tcaggctgca tgtcattgcc aatagcaact ctcctgaaga ccaagagtta aagctcaaag
     7801 tgagagacgc aatcaattca gccctta
//
LOCUS       DAONUO010000061         7679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7231_length_7679_cov_3.713274, whole genome shotgun sequence.
ACCESSION   DAONUO010000061 DAONUO010000000
VERSION     DAONUO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7231_length_7679_cov_3.713274"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK768_03435"
     CDS             complement(<1..226)
                     /locus_tag="PK768_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HQA60052.1"
                     /translation="MRQAYQLEWASKDLGQESRVALGDVNGDGRINIIAGTTAASGHQ
                     SNLYVFQYRGNTYHQLARTSLENNDTHWLCC"
     gene            complement(316..1335)
                     /locus_tag="PK768_03440"
     CDS             complement(316..1335)
                     /locus_tag="PK768_03440"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQA60053.1"
                     /translation="MKMKKYNIAIVGATGAVGQTMLKILEERDFPVNRVLPLASARSN
                     GISVSFRGEKLKVLEATPASFNGIDIALFSAGKTVSKELAPEAIKRGAIVIDNSNAFR
                     MSPEVPLVVPEVNPEDIKKHKGIISNPNCSTIQMVVALKPLHDVARIKRVVVSTYQAV
                     SGTGLEAIEELMEQLKMVLNGEDPKARVYPYPIALNVLPHIDVFDDTGYSQEELKMVN
                     ETKKIMNDDNLAITATTVRVPVMNGHSEAVNIETEEKLTAEMAREILSKASGVKVVDD
                     PKSKRYPLPGDVTGSDEVFVGRIREDFSVRNGLNLWIVADNLRKGAALNTIQIAEKMV
                     ENAMI"
     gene            complement(1692..2867)
                     /locus_tag="PK768_03445"
     CDS             complement(1692..2867)
                     /locus_tag="PK768_03445"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQA60054.1"
                     /translation="MGLIVQKFGGTSVKSAESRKAVLGHILKAKEQGHDVVVVVSAMG
                     RRGDPYATDTLIDLLREQGGKINPKTQDLIMSCGEIISACVMATYIEAQGVKAEAMTG
                     FQAGILTTEDFGNADIIKVYPEQIMEKLKEGKVVVVAGFQGRTEKNDITTLGRGGSDT
                     TAVVLGGYLKADLVEIYTDVPGIAVTDPRLVPEAQFIPRLSYEETITLAENGAKVIHP
                     RAVKAAREFDLKLRIKSTFDESEGTLVGPERSDLIVSGISLTRDFTAARYEREKTFSD
                     DYVKKLQGLGIDKVIYEDDAGQKLVLINEEDKDAAGSLANELNAEVTFLESMSKVTVV
                     YKDTVCSKAVDEKIASSLSQQDIPVLAKKVQTNFSSYIVKNDLAEKCVKVIYKAYFN"
     gene            complement(2925..3434)
                     /locus_tag="PK768_03450"
     CDS             complement(2925..3434)
                     /locus_tag="PK768_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQA60055.1"
                     /translation="MIGNFKNLRPKIHESCFIAPTADVIGDVTVGENSSIWHRTVLRG
                     DVNKIVVGANTNIQDGSIVHVADDYPTIIGDNVTIGHGAIVHGCTVKDGAFIGMGAII
                     LDGAVIGEGALIGAGALVTEGKEIPPYSLAVGVPAKVVRELSQEYIEMAQDRAVKYAK
                     LANEYKELK"
     gene            3817..7221
                     /locus_tag="PK768_03455"
     CDS             3817..7221
                     /locus_tag="PK768_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQA60056.1"
                     /translation="MLHILKNSLHKRLIPWILTILISASALMPAFGQEVLLYEESTGF
                     PVTGGVTYEVKTLFTSQGWQKIHILRVNLDSDNVDIDTIIGKNGLSQRDSLNRMVQDN
                     GAVAGINGDFFITATPSAPIGPQISNGKLISTPLNDMDMAAIGLSFDRIPEILKLEFS
                     GRLIAPDRSYFTVEGVNKIRNSYNNIFVYTPEFGSTTPKPGEGAPNLTFATVKDNRIV
                     SISEGQTAPIPEDGLVLMAWGDGGNFLKTRFSTGDHIELDLQITPDVSNLKMLLGGGA
                     VLVENGQIPKVFSHDVPGTHPRTAIGFTADKKTIIMVVVDGRQAQSRGMTQQELAELM
                     LSLGAYDALNLDGGGSSTMVVKPFDEIQPRVVNSPSGGVQRLIPNALGIFAKAPVGEI
                     YGLKIKASSFNIAKGGNRTFDVIAYDENYNIVDINPDKVRWSVTGDIGYFNGNVFTAQ
                     KSGTAQIVASYGDIQARQDIRVLKDAVMLKIEPEKVHVNPGGKALINVCVIDSEGFKA
                     PLESNDVKFNLVGEVGTINGLEFTASQMPTSGAIIAEFAGLQAGALVQTGYQSILLDD
                     FESIQGKAFSSYPAYVKGSFSITSLPDPVYSGQFSGKLAYDFTGGTDTRAAYLAFQNG
                     GRTLPEGTVKIGLRVYGQGQGEWMRAAVEDASGKETILDISKSVDWQGWRWLEVNLPA
                     GKAPFKLKTIYVVETNPDIKTTGALYLDDLTAFAAGMYDESLVPASPIWIDAANQKGS
                     GFTFGALGAMPDDNQNNLSTLALAKDILNKHGASLNVILGKPWADEATLEEQLDGLNN
                     YRFSGTGYSAYMAQDAIFIFLDASKGGLRATDYKQWLNLQNDLKSITSKTLFVVIDRA
                     PENFSDSLEGELLKKVLSEHAKSTGQAVWVLTNSQTAFSSKAEDGVHYLSIPSINAKE
                     PAAVLFYLANGRVNYQVIPLVSRIINETPGVKAGVATTLKIYGITAEGNKIHLGYPYA
                     AEWRVTYDKPVVFDPKSLSFKVDSPGTANLQLIMGESLHSFSIMISDITVLVNGQEVH
                     FPDQVPYINKDNRTMVPVRFVSESLGAKVSWDNDNRMVIIEKGQDLIKLKIGENKAVV
                     NGETVYFDTTAVLQNDRTMVPVRFISEALGAKVGWNQEKRAVEIEY"
     gene            complement(7318..>7679)
                     /locus_tag="PK768_03460"
     CDS             complement(7318..>7679)
                     /locus_tag="PK768_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60057.1"
                     /translation="ALIEAEAYPGPSLIIAYAPCINHGLKLGMGSTKQEEKRAVEAGY
                     WHLYRYNPQLKEEGKNPFILDSKEPTADFKEFLMGEVRYSSLVNAFPDRAEELFEKAA
                     KDAKERYETYKRLAETM"
BASE COUNT         2094 a         1814 c         1608 g         2163 t
ORIGIN      
        1 gacagcagag ccagtgagta tcgttgtttt ccagggaagt tcgggccagc tgatggtaag
       61 tattgcctcg gtattgaaat acgtacaaat tggattggtg cccggaagca gcagtcgtcc
      121 ctgctatgat atttatgcgg ccatcgccgt ttacatcgcc cagcgccacc cgtgattcct
      181 ggcccaagtc cttactggcc cattccaatt ggtaagcctg cctcatttac atctccctcc
      241 ccaaaagcaa agcacattaa tgctgttcct tatatactat tcgggagaga gaaaaaagga
      301 ctacaataat taaactcaaa tcatagcatt ttccaccatt ttttctgcaa tctgtatggt
      361 atttagcgcc gcaccttttc tcaggttatc agccacaatc cataagttta aaccgtttct
      421 tacggaaaaa tcttctcgta ttcttcctac aaaaacctca tcactgcccg tcacgtcgcc
      481 ggggagagga tagcgtttgg atttaggatc atctactacc ttaacccccg atgccttgga
      541 gaggatttcc cgggccattt cagctgtcag tttttcttca gtttcaatat ttactgcttc
      601 cgagtggccg ttcatgacag gcaccctgac ggtagtagca gttatagcta gattgtcgtc
      661 attcattatc ttctttgtct cgtttaccat ttttaattct tcctggctgt accccgtatc
      721 atcaaacaca tcaatatggg gcaggacatt taaggcaata ggataaggat agacccgagc
      781 tttaggatct tccccattta acaccatttt taactgctcc attagttcct caatagcttc
      841 taaaccggta ccggataccg cctgatatgt agagacaacg actctcttaa tcctggccac
      901 atcatgtaag ggttttaatg caaccaccat ctgaatggta gagcaatttg gattcgatat
      961 aatgcctttg tgttttttta tgtcttccgg attcacctca ggcacaacca gggggacttc
     1021 gggagacatg cgaaaggcat tgctgttatc tatgacaatt gcaccacgct ttatcgcctc
     1081 aggtgcaagc tccttgctta cagttttacc tgcagaaaaa agtgcaatat caatgccgtt
     1141 aaatgaagca ggagtagctt ccagcacctt tagcttctcg cctctgaagc taaccgatat
     1201 gccatttgac cgggcagaag ctaagggcaa taccctgttt accggaaaat ctcgttcctc
     1261 caaaatcttc agcatagttt gacccacagc ccccgtagct ccaactatag cgatgttgta
     1321 ttttttcatt ttcatccact cctttatctg ttgtaataat taagcatttt cgatgagctg
     1381 ggccgctcac catttttata tttccattat tttaatataa gcttatgtaa aatgcaatat
     1441 cttcttgaag tttaactttt ctgttaaaaa cataagccgg attttgtcta tgtgctgcgg
     1501 acatttgtat ttgattcaag aacgttagca tattacattc gacacggagc ttcaataccc
     1561 ttttttttga attattttgc tagtttggat tttatctata aaaaaagctg ccatttaaaa
     1621 tgacagcttt ggcatgatta tatgagcttt tagcaaacat gtcaggataa agcaatgaaa
     1681 cggccgtttt tttagttaaa ataagcctta tagataactt ttacgcattt ttcggccaaa
     1741 tcatttttca caatataact gctgaagttt gtctgtacct ttttggcaag taccggaata
     1801 tcttgttggc tgagggatga tgctattttc tcatcaacgg cctttgagca aacagtatcc
     1861 ttataaacaa cagtcacctt tgacatgctc tccaaaaaag tcacttcagc atttagttca
     1921 ttagctaaac ttcctgctgc atctttatcc tcttcattta ttaataccag tttctggccc
     1981 gcatcgtctt cataaataac tttgtctatg cccagccctt gaagtttttt tacataatca
     2041 tccgaaaagg ttttttctct ctcatatctt gccgcggtga agtcccttgt tagggaaatt
     2101 ccggagacaa tcaggtcact tcgttcaggc cccaccaggg tgccctccga ttcgtcaaaa
     2161 gtggatttaa tcctaagctt caaatcaaac tccctggcag ccttgacggc tctcggatga
     2221 attactttgg caccgttttc cgccaatgta attgtttcct cgtatgataa cctcggtata
     2281 aactgcgctt cagggaccag ccgagggtcc gtaaccgcga ttcccggcac atccgtgtat
     2341 atttccacca aatccgcttt cagatatcca cccaacacca ccgcagtggt atcgctaccc
     2401 ccacggccca aggtggtaat atcatttttt tctgtcctac cctgaaaacc tgcgactacc
     2461 acaactttgc cttcttttaa cttttccatg atctgctcgg ggtaaacttt aatgatatcg
     2521 gcattgccaa aatcctccgt ggtgagtatc cccgcttgaa atccggtcat ggcttcggcc
     2581 ttaacccctt gtgcctctat gtaagttgcc ataacacatg ctgaaataat ctccccgcag
     2641 gacatgataa gatcctgtgt tttgggattg attttacccc cctgctccct caataggtcg
     2701 attaaagtat ctgtagcata cggatctccc cttctgccca tagctgacac cacaaccacc
     2761 acatcgtggc cctgctcttt tgccttcaag atgtgaccta gcactgcttt tcggctttca
     2821 gcagatttta ccgatgtacc accaaacttt tgcactatta ggcccatatg acacctccaa
     2881 attattattt tatagtgtaa taagtgtcga gtaatgtgtc aacactactt aagctcttta
     2941 tattcgtttg ccaatttggc atatttaacg gctctgtctt gtgccatctc gatgtactcc
     3001 tgggatagtt ctcttacaac tttagcagga actcctacag ctaaagaata gggcgggatc
     3061 tccttgcctt ccgtgacaag ggcacctgca ccgattaatg ccccttctcc tatcactgcc
     3121 ccgtcaagaa ttatggcacc catgccgata aaggcgccat cttttaccgt gcagccgtgg
     3181 acaattgccc catggccaat agtgacatta tcgcctataa ttgtaggata gtcatcagcc
     3241 acgtggacaa ttgagccatc ctgtatgttg gtatttgcgc caactactat cttgttcaca
     3301 tcaccccgaa gcaccgtcct gtgccatata cttgaattct cacccactgt cacatctcct
     3361 atcacatcag cagtaggcgc tataaagcag ctttcgtgaa tcttgggcct taaatttttg
     3421 aagttaccta tcataagaac accacctttt tgaatgaata tttcgggacg ggtacaaaat
     3481 attcacgagt tgtgaaatac tatgaaatat tatagcatgt tacgtttagt attgacattt
     3541 gttaccagta aggaagttcg ctcaggttat tcgcttactt gcatcacctg acgccgtgga
     3601 tacccttacg ataaaaagat agctgatacc tatttccaat gttactatat ttttactcca
     3661 gatggaacct ttaacttttt tacacgtctg atataataga aacatagttt ttcaagcatt
     3721 ggatatagtt ttacctgggg gatcagaaaa caaggaggga tttttaactc aggaaacttt
     3781 atttgaatta atgcactgaa acaggaggga agttacatgc tacatattct aaaaaatagc
     3841 ttgcataaac gccttattcc ttggattctg accatcctaa ttagtgcctc tgcactaatg
     3901 ccggccttcg gtcaggaagt cctgctctac gaagaatcga caggttttcc ggtaaccggt
     3961 ggtgttacat atgaagtaaa aactctattt acctctcaag gctggcaaaa aatacacatt
     4021 ttaagggtaa acttagactc tgataacgtc gatatagata caatcatagg aaaaaacggc
     4081 ctttcccaaa gagattcctt aaaccggatg gtacaggata acggtgcggt ggccggcatc
     4141 aacggtgatt tctttattac ggccacacct tctgccccaa tcggacctca gataagcaat
     4201 gggaaactta tttccacgcc gcttaacgat atggatatgg cagcaatcgg ccttagtttt
     4261 gataggattc ctgaaatact aaagctagag ttttcgggta ggctgattgc ccccgaccgc
     4321 agctacttta ccgttgaagg tgttaataaa atcagaaaca gttacaacaa tatattcgtg
     4381 tacaccccgg aattcggttc cacaacaccc aagccgggtg agggtgcccc taacttaacc
     4441 tttgcaacgg taaaggacaa ccgcattgtc agtatttcag aaggccaaac tgccccaata
     4501 cccgaggacg gtttggtgct gatggcttgg ggagacggag gaaatttcct gaagacccgc
     4561 ttttctaccg gcgaccacat tgaactggac cttcaaatca ccccggacgt ctcaaatctg
     4621 aaaatgctct tgggtggtgg cgcagtttta gttgaaaacg gccaaatacc caaagtcttt
     4681 tcccatgacg ttccgggaac tcatcccagg actgctatcg gctttaccgc cgacaaaaaa
     4741 accataatca tggttgtagt tgacggccgc caggcccaaa gccgcggtat gacacagcag
     4801 gaactggcag agcttatgtt gagccttggt gcatatgatg cgctgaatct tgacggcggc
     4861 ggctcctcca ccatggtggt aaaacccttt gacgaaatcc aaccgagagt tgtcaacagt
     4921 ccctcgggag gtgttcaacg actaattcca aatgccctgg gaatttttgc taaggcaccg
     4981 gtaggtgaga tttatggtct taagataaaa gcttcttcat tcaatattgc caagggcggt
     5041 aacaggacct ttgatgtcat agcctatgat gaaaactaca acatcgttga tattaacccc
     5101 gataaagttc gctggagcgt caccggggac attggttact tcaatggcaa tgtttttaca
     5161 gcccaaaaaa gcggcacagc acagattgtt gctagctacg gagatattca ggcaaggcag
     5221 gatatcaggg tgttgaaaga tgccgtcatg ctgaaaattg aacccgaaaa ggtgcacgtg
     5281 aatcccggag gcaaggccct gataaacgtc tgtgtaattg acagtgaagg atttaaagca
     5341 ccacttgaat ctaatgatgt aaagtttaat ctcgttggcg aagtcggtac aataaatggc
     5401 ctggagttta ccgctagcca aatgccaaca agcggtgcca taatagcaga atttgcaggc
     5461 cttcaagccg gtgcattggt gcaaaccggc taccaaagta ttttgcttga cgactttgaa
     5521 agcatccagg gcaaagcctt ttcatcttac ccagcatacg taaaaggttc cttcagtatt
     5581 acatcactgc cagacccggt atactccggc cagttttccg gcaaattagc ttacgatttt
     5641 accggaggaa ctgatacaag ggcagcctat cttgccttcc aaaacggcgg cagaactttg
     5701 ccagaaggca ccgtcaagat tggccttcgg gtctacggac aaggacaagg agagtggatg
     5761 agggctgccg tggaggatgc ttcaggcaag gaaaccatac tcgatatttc aaagagcgtt
     5821 gactggcagg gttggcgctg gcttgaggta aatctacctg ccgggaaagc gccctttaag
     5881 ctcaagacca tctacgtggt agaaaccaat cctgacataa aaactactgg ggccctttac
     5941 ctggatgacc ttacagcttt cgcagcaggt atgtatgatg aatcgctagt tcctgctagc
     6001 ccaatttgga ttgatgctgc aaatcaaaag ggcagcggtt ttacctttgg agccttggga
     6061 gctatgccgg atgacaatca aaacaattta agcacactgg ccctagccaa agatatttta
     6121 aataagcatg gtgccagctt aaatgtcata ctaggcaagc cctgggctga tgaagccaca
     6181 ttggaagagc agctggatgg cttgaacaat tatagattct ccggaaccgg atactccgcg
     6241 tatatggcac aggatgctat ttttatcttt ttggatgctt ctaaaggcgg cttgcgtgct
     6301 accgactaca agcagtggtt gaatctgcaa aatgacttaa aatccatcac atccaaaact
     6361 ctttttgtgg tcatagatag ggctccggaa aacttttcgg attccctgga aggtgaactg
     6421 ctaaagaaag tccttagcga acatgccaaa tccacgggcc aggccgtatg ggtgctgaca
     6481 aatagccaga ctgcttttag cagcaaggct gaagatggtg tacactacct ttcaattcct
     6541 agtataaatg caaaggaacc agctgcagta ctgttttacc ttgcaaacgg cagagtaaac
     6601 taccaggtca ttcccctggt gagcagaatc attaatgaaa caccgggagt taaggccggg
     6661 gtagccacaa ctcttaaaat atacggaata acagccgaag gaaataagat acacttgggt
     6721 tacccctatg ccgctgagtg gcgggttaca tatgacaagc ccgtagtctt tgaccccaaa
     6781 agccttagtt tcaaggtaga cagtcccggc actgcaaatc ttcagctaat aatgggggaa
     6841 agtttgcatt ctttctccat tatgataagt gacataacag tcctggtaaa tggccaggaa
     6901 gtgcatttcc ccgaccaggt accttacatc aataaagata acaggaccat ggttcccgta
     6961 agatttgtct ccgaaagcct tggagcaaag gtcagttggg acaatgacaa caggatggta
     7021 atcatcgaga aaggccaaga cctaataaag ctcaaaatcg gtgaaaacaa ggctgtcgtc
     7081 aacggcgaaa cagtatactt tgacacaaca gccgtcctgc aaaatgacag aaccatggtg
     7141 cccgtaagat tcatcagtga agccctcggg gcaaaagttg gctggaacca ggaaaaaaga
     7201 gccgtagaaa ttgaatacta aggtaacttg ccctgtcttt taaagagacg gggctttttc
     7261 atttatacga aaagagcacc aacccttttt ggattggtgc tctaaattgc ctatatttta
     7321 catcgtctct gccagtcttt tataagtctc atagcgttct ttggcatcct ttgcagcttt
     7381 ttcaaagagt tcctcagccc tgtcagggaa ggcgtttacg agggacgagt acctgacttc
     7441 acccattaag aactccttga aatcggcagt aggttcttta gagtcaagga taaagggatt
     7501 cttgccttct tctttaagct gcggattgta cctgtacaga tgccagtaac cggcttcgac
     7561 tgctctcttt tcttcttgct tggttgaacc cattccaagt ttcagaccat ggttaataca
     7621 aggagcgtag gcaatgataa gtgatggtcc cggatatgct tctgcttcta taagtgctt
//
LOCUS       DAONUO010000062         7636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7283_length_7636_cov_4.076111, whole genome shotgun sequence.
ACCESSION   DAONUO010000062 DAONUO010000000
VERSION     DAONUO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7283_length_7636_cov_4.076111"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>781)
                     /locus_tag="PK768_03465"
     CDS             complement(<1..>781)
                     /locus_tag="PK768_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQA60058.1"
                     /translation="EKFFTGSNIEVDRVYTELDIQDFDYVNDLGFPGEYPYTRGVQPT
                     MYRGRLWTMRQYAGFGTAEESNKRYKYLLSQGQTGLSVAFDLPTQIGYDSDHPLSQGE
                     VGKVGVAIDSLKDMEILFEGIPLDKVSTSMTINAPAAILLAMYIALAEKQGITPDGLS
                     GTIQNDILKEYIARGTYIFPPEPSMRLITDIFEYCSAHMPKWNTISISGYHIREAGAT
                     AVQEVAFTLANGIAYVEAAIKAGLSVDDFAPRLSFFFNAHND"
     assembly_gap    782..791
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1074..2339)
                     /locus_tag="PK768_03470"
     CDS             complement(1074..2339)
                     /locus_tag="PK768_03470"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQA60059.1"
                     /translation="MSEKINPVESVQNEIKLACEKLGLDNSYYEILKEPERVLIVQVP
                     VRMDDGTIKTFTGYRAQHCTIMGPAKGGFRYHPDVNLDEVKGLAMWMTFKCAVVGIPY
                     GGAKGGVCCNPAELSKAELERLTRSYLRAINTMVGPDKDIPAPDVNTNPQIMAWFMDE
                     FSMLKGHNVPGVVTGKPISLGGSQGRVQATGFGVTVAVKKACEAMGIDVSKTNIAIQG
                     FGNVGSYTALYCSKNGAKIVAVGEWDKDIGTYALYDEEGLDIEKLFDYKAKNGTIVNY
                     PDARRINLDEFWTLDNVDILIPAALENAIDENNAPNIKAKIIVEAANGPTTPEADRIL
                     AEKGIALFPDILCNAGGVTASYFEWVQNLMNFYWTVEEVNTRLEEILTKAFDDVYNMH
                     KEAGVTLRQAAYLVAINRIAENMKLRGWV"
     gene            complement(2367..2897)
                     /locus_tag="PK768_03475"
     CDS             complement(2367..2897)
                     /locus_tag="PK768_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQA60060.1"
                     /translation="MDERIEIRLSGSGGQGLILAGIILAEAAILDGKNAVQTQSYGPE
                     ARGGASRSEVIISNASIDYPKVSKSDILLALTEEAMLKYKSNLLDDGLLIIDSSIDMP
                     DGPFKILSVPIIKTAQEKVGKTIVANIVALGTLVAATNIVSKESIEKAILARVPKGTE
                     ELNKKALYEGYNLVNI"
     gene            complement(2899..3717)
                     /locus_tag="PK768_03480"
     CDS             complement(2899..3717)
                     /locus_tag="PK768_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQA60061.1"
                     /translation="MRKELEKYFRTDKLPHIWCPGCGHGIITGAVIRAIDNLKLDQDK
                     ICIVSGIGCSSRAPGYMNFYTLHTTHGRALAFATGIKMAKPELKVIVLTGDGDCSAIG
                     GNHLIHAARRNIDITTIVFNNSIYGMTSGQYSPMTPKGAFATTAPYGNIDRDFDLCKL
                     AQSSGATYVARGTVYHLPQLIKLIEKALKHNGFSFVDVVDICPTYFGRRNKMGTAVAM
                     METIREKSVTIRTASKMSAEELSGKIVIGEFYEENAPEYTEEYAKIIEKAQGRI"
     gene            complement(3710..4858)
                     /locus_tag="PK768_03485"
     CDS             complement(3710..4858)
                     /locus_tag="PK768_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQA60062.1"
                     /translation="MSNRVALMQGNEACVEGAIAAGMRFYAGYPITPSTEIAELSASR
                     LPKVGGKFIQMEDEIASMGAVIGASLAGLKAMTATSGPGFSLKQENIGFACLTEVPCV
                     IVNVQRGGPSTGLPTLPAQADVMQARWGTHGDHPIIVISPSTVKEVFDMTIRAFNLSE
                     KYRVPCILLMDEVVAHMREKVELPEELNVEIIDRKKPDPAISNYKPYKDYDGDGIPPM
                     ACFGDGIPFHVTGLIHDETGFPSTNPVVTENLLNRLMSKIYNNLDDIIEYEMIDAEDA
                     DTVIVSFGSTARSASRAVNLLRQEGLKVGMLRLKTIWPFPHKAINELKQWAAKNLIVA
                     EMNYGQLIDVVKASAYGTFKVYGLNKYNGELITPEEVMDKVKEVAANA"
     gene            complement(4848..5057)
                     /locus_tag="PK768_03490"
     CDS             complement(4848..5057)
                     /locus_tag="PK768_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA60063.1"
                     /translation="MTAKNIRILIDEKACKKCGLCIAFCPKKVFISENGKPVIANLSA
                     CIDCKLCELRCPDYAIIVGGEEIEQ"
     gene            complement(5068..6015)
                     /locus_tag="PK768_03495"
     CDS             complement(5068..6015)
                     /locus_tag="PK768_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQA60064.1"
                     /translation="MVNFEADKTPLYIQIKNHIRKYIEDGNWPIGHKLPSERELAREM
                     GVSRKTVSLAYKELVDEGILSSHRGRGTFVLELPKNNNPSYNNIIENIDRCINSCLKM
                     GIDPDTFLKLCRKRVNEYKEKLHELKIILIECNKEQLDYFSKELELGAGAAITPVLLQ
                     DFKNNLGNIQQKLSDYDYLITTLFHIDEVKKIVNERDINILPIALNPQLESVIKIARI
                     PKESTIGILTISENFANKVKRAIFDAGLDFKEIIISTSTDFSEIKKLIEKVDALIVSP
                     GRKKHVIPFVKHQHVIEFIFVPDAGSINLLNTSITKKTK"
     gene            complement(6168..6983)
                     /locus_tag="PK768_03500"
     CDS             complement(6168..6983)
                     /locus_tag="PK768_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA60065.1"
                     /translation="MKVDLHLHTTCSDGLFTPQQLVKKAYELNLKAIAITDHDTVDGI
                     LPAIIEANKYKDLEVIPGIELSTHCNGQEVHILGYYIDYENFHLKEQLLDLQQNRIKR
                     LEKILTKLNDIDIQIKYDDVYKYSRGTSVGRPHVALALVEKGYVSSIKEAFDKFLAKG
                     RPAYVPRKKLTPIDAIKLIKECRGIPVLAHPGLLEDDSIISELITNGIMGIEVIHKDH
                     TKDDTAYYTKLALENNLLLTGGSDSHGEEPLLLGTLDIPFKYAARLKDTAKVI"
     gene            complement(7253..7513)
                     /gene="spoVS"
                     /locus_tag="PK768_03505"
     CDS             complement(7253..7513)
                     /gene="spoVS"
                     /locus_tag="PK768_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein SpoVS"
                     /protein_id="HQA60066.1"
                     /translation="MEVLKVSAKSNPNSVAGALAGVLRERGAAEIQAVGAGALNQAVK
                     AVAIARGFVAPSGVDLICIPAFTDIEIDGQERTAIKLIVEPR"
BASE COUNT         2072 a         1650 c         1326 g         2578 t
ORIGIN      
        1 gatcgttgtg tgcattgaag aaaaacgaaa gtcttggtgc aaaatcgtca acggaaagac
       61 cggcttttat tgcagcttca acatacgcta ttccatttgc aagtgtgaat gccacttctt
      121 gaacagcagt tgcccctgct tctctaatat ggtaaccgct aatactgatg gtgttccatt
      181 tgggcatatg agctgagcaa tattcaaata tgtcggtgat aagtctcatg gatggttcag
      241 gaggaaagat atatgtacct cttgcaatgt attcctttag aatgtcattt tgaatggtgc
      301 ccgataagcc atcaggtgta atgccttgtt tttccgcaag tgctatatac atggccaaaa
      361 gaattgctgc cggggcatta atcgtcatag atgtgcttac tttatccaag ggaatgcctt
      421 caaacaaaat ctccatgtct tttaatgagt caatagcgac accaacttta ccaacttcac
      481 cttgagatag cggatgatcc gaatcgtatc caatttgagt cggtaaatca aaggctacac
      541 ttaagccggt ttgtccctgt gataagagat atttgtacct tttgttggac tcttctgccg
      601 tcccaaatcc tgcatactgc cgcatagtcc agagtctgcc cctgtacatt gttggctgaa
      661 ctcccctggt ataaggatac tcgccgggga atcctaagtc attaacataa tcaaagtctt
      721 gtatatctaa ttctgtatac acacgatcaa cttctatatt tgaacctgta aaaaatttct
      781 cnnnnnnnnn ntttctttcc ggcctcttat tcaaaacctt gttcaaggtg ttcttttccc
      841 aattttcttt atgctgactt attttgctga gggtctcctt ttttatcatt gcaatttgcc
      901 ctcccattat tattctttta tataatacac tatttttcct gtttaggcaa taaaagcttt
      961 ttataccctg aaagaactgt aatatcggtt tacccaatat ccccgctctt aaaggcgagg
     1021 atattaggtg ttgtataaca tcaaaaaatt ttcaattaac attgacagga tttttatacc
     1081 cagcctctta atttcatatt ttctgctatc cgattaatgg caactagata agccgcttgt
     1141 ctcaaggtaa caccagcttc tttgtgcata ttatatacat catcaaaggc ttttgttaat
     1201 atttcttcca gtctcgtatt tacttcttct acagtccagt aaaaattcat caggttctgc
     1261 acccattcga aatatgaagc agtgacacca cctgcattgc aaagtatgtc agggaacaga
     1321 gctatgcctt tttcggctag aattctgtca gcttcagggg tagtaggacc gttggcagct
     1381 tcaacaatga ttttagcttt tatattggga gcattatttt cgtctatagc attttcaaga
     1441 gccgcaggaa taagtatgtc tacattgtca agggtccaga attcatcaag gttaatcctc
     1501 ctggcatccg ggtaattgac aattgtacca tttttggctt tgtaatcaaa gagcttttca
     1561 atatcaagac cttcttcgtc gtatagcgca tatgttccta tatctttgtc ccactctcct
     1621 actgctacta tttttgctcc gtttttgctg cagtataatg cagtatagct tcctacatta
     1681 ccaaaacctt gaattgcaat atttgttttg cttacgtcta ttcccattgc ttcacaggct
     1741 ttttttactg ctaccgttac tccaaatccg gttgcttgta cccttccctg tgaacctcct
     1801 agtgatatag gctttccggt aacaactccc ggaacattat gaccctttaa catgctaaac
     1861 tcatccataa accaggccat tatttgtgga tttgtattca catccggtgc agggatatct
     1921 ttatcaggtc ctaccatagt attgattgct ctcagatagc tgcgtgtaag tctttccaat
     1981 tcagccttgg acagctctgc aggattgcag cagactcctc ccttagctcc accgtacgga
     2041 attccaacta cggcgcattt aaaggtcatc cacatggcaa gtccttttac ttcatcaaga
     2101 ttgacatcgg gatgatatct aaaaccaccc ttggcaggcc ccattattgt gcagtgctgg
     2161 gcgcgatatc cggtaaaggt ttttatggtt ccatcatcca ttcgtactgg aacttgaact
     2221 atcagcaccc gctcaggttc ttttagaatc tcataatacg agttatcaag accgagcttc
     2281 tcacaagcaa gtttaatttc attttgaaca gattctacgg ggttgatttt ttcagacatt
     2341 tgaaaaactc ccttcatgat aaaattttat atgtttacta agttataacc ttcataaagg
     2401 gcttttttgt ttagttcctc ggtgcctttc ggaactctag ccagtattgc cttttcaata
     2461 gattcttttg aaactatatt tgttgctgca accaaagttc ccaatgctac aatatttgca
     2521 acaattgttt tcccgacttt ttcttgcgca gttttaatta taggaacact tagaatctta
     2581 aaaggaccat ctggcatgtc tatgcttgaa tctatgatta gcaaaccatc atccaggaga
     2641 ttacttttat attttaacat agcttcttca gttaatgcta gtagtatatc agattttgat
     2701 acttttggat agtcaatact ggcgttgcta atgataactt ccgatctact ggcaccaccc
     2761 cgcgcctccg ggccgtacga ttgggtttga acagcgtttt tgccgtctag tatcgcagct
     2821 tcagcaagta tgattccggc tagaattaag ccctgacccc ctgaaccgct gagtctgatt
     2881 tcaattcttt cgtccacctt aaatcctccc ttgcgctttt tctataattt tcgcgtattc
     2941 ttcggtgtac tcaggcgcat tttcttcata aaattcacct atgacgatct tcccggagag
     3001 ttcttcagcc gacattttcg aagcggtcct tattgtaact gacttttctc gaattgtttc
     3061 catcatagcg acagcagtcc ccattttatt tctacggcca aaataggtgg gacatatatc
     3121 tactacatct acaaatgaaa atccattatg ttttagggct ttttctatta gttttattag
     3181 ttgtggaaga tgataaaccg ttccccgggc tacataagtg gcaccagaag attgggccag
     3241 tttgcatagg tcaaaatctc tatcaatatt gccgtagggt gctgttgtcg caaaggctcc
     3301 cttaggtgtc ataggtgaat actggccgct ggtcataccg taaatgctat tgttaaacac
     3361 aatcgtcgta atatctatgt ttctacgagc agcgtgaatt aagtgattgc caccgatggc
     3421 tgaacaatcg ccatcacctg taagcactat gacctttaat tccggctttg ccatcttgat
     3481 tcctgtagcg aaagcaaggg ccctaccgtg ggtggtatgc aaagtataaa aattcatata
     3541 tccgggagct ctcgatgagc atccgatccc ggacacaatg catattttgt cctgatccag
     3601 ttttaaattg tctatagcgc ggataacggc acctgttatt atcccgtgac cgcagccagg
     3661 acaccaaatg tgcggtagct tgtcagttct aaaatatttt tccagctctt tacgcatttg
     3721 cggctacctc cttaacctta tccattactt cctcaggcgt aataagctcc ccattatact
     3781 tatttagtcc ataaacttta aatgtaccat aagccgaggc tttaacaaca tctatcaact
     3841 ggccgtagtt catttctgca actataagat tcttagcagc ccattgcttc aattcgttta
     3901 tagccttgtg gggaaaaggc catatggttt ttaatctgag catgcctacc ttaagaccct
     3961 cttgccttaa aagatttact gcccttgatg cagaacgtgc agtagaaccg aagcttacta
     4021 tgactgtatc tgcatcttct gcatctatca tctcatattc aataatatca tctaaattgt
     4081 tatagatctt gctcataagt ctgttcagta aattttccgt aacgacggga ttggtagatg
     4141 ggaagccagt ttcgtcatga atcaggcccg taacatgaaa cggaatacca tcgccgaagc
     4201 aggccattgg tggtatgccg tcaccgtcat aatccttata cggtttataa ttggatatag
     4261 caggatcagg tttttttctg tctattattt caacgtttaa ctcttccggc aattctactt
     4321 tttctctcat atgtgctaca acttcatcca taagcaggat acaaggaact ctgtactttt
     4381 ctgataagtt aaaggccctt atagtcatat caaatacttc ttttacagtg gacggagaaa
     4441 tgactataat cggatgatct ccatgagtac cccacctagc ttgcattaca tcagcttgag
     4501 ccggtaatgt aggcaatcct gtactaggtc ctcctctttg aacattaact atgacgcatg
     4561 gaacttccgt aaggcaagca aatcctatgt tctcctgttt aagcgaaaat ccaggaccgc
     4621 tggttgcggt catggcttta agaccggcga gggatgcacc tataaccgca cccatgctgg
     4681 ctatttcgtc ttccatctgg atgaattttc ctcctacttt aggaagcctt gatgctgaaa
     4741 gttccgcgat ttcagtagag ggtgtgatgg gatagccagc ataaaaccgc atacctgcgg
     4801 ctatagcacc ttcaacgcat gcttcgttac cttgcattaa tgctacccta ttgctcaatt
     4861 tcttcacctc cgactattat tgcataatct gggcatctca attcacacaa tttgcaatcg
     4921 atgcatgcac ttaaattagc tattaccggt ttcccgtttt cagatatgaa tacttttttg
     4981 ggacaaaagg ctatgcagag accacatttt ttgcaagcct tttcatcaat taggattcta
     5041 atattttttg ctgtcaaaat atcacctcta tttggttttt tttgtaatgg atgtatttaa
     5101 taaattaatt gagcccgcat cgggaacaaa aataaattca ataacatgtt ggtgtttcac
     5161 aaaaggtatt acgtgttttt ttctgccagg tgaaacaatc aaagcatcta ccttttctat
     5221 taacttttta atttcggaga aatctgtgct tgtagaaata attatttcct taaaatctag
     5281 ccccgcatcg aaaatcgctc ttttaacttt gtttgcaaaa ttctctgaaa ttgttaagat
     5341 gccaattgta ctttctttag gtattctggc tatttttatg acactttcta attgaggatt
     5401 caatgctatt ggaaggatgt tgatatctct ttcattaaca atcttcttaa cttcgtctat
     5461 gtgaaataaa gtggttatta aataatcgta atcactgagt ttttgttgga tattacctag
     5521 attatttttg aaatcttgta acaatacagg tgtgatagct gcacctgcac ctaattcaag
     5581 ctctttacta aagtaatcta actgctcctt attgcattct ataaggataa ttttcaactc
     5641 atgaagcttt tccttgtatt catttactct ttttctacat agttttaaga aagtgtcagg
     5701 atcaattccc atcttcaggc aagaattaat gcatctatcg atgttttcga ttatattatt
     5761 gtagcttgga ttattatttt ttggcaattc taagacaaaa gtccctcgcc ctctgtgcga
     5821 tgacaggatg ccctcatcta ccaattcttt ataagcaagg cttacagtct ttctgctgac
     5881 acccatttct ctggccagct ctctctcgga aggtagttta tgaccaatag gccaattgcc
     5941 atcttcgata tatttcctta tatggttttt tatttgaatg taaagaggag tcttatctgc
     6001 ttcaaagttt accatttgca tcaccaaaag gattattggc tcaaaaggag tcctttctat
     6061 aaatatattc gacaaatgtt ggaatattcc tgctgaagac gcaaaaaaaa taagccaatt
     6121 atgaatattt ggctctttgt atacaagaat attatatttg ttgtagacta gataactttt
     6181 gctgtgtctt tcagtctggc agcatactta aagggaatat ccagggtgcc taataggagt
     6241 ggctcctccc cgtgggaatc ggaacctccc gtcaacaata aattattttc cagggctaat
     6301 tttgtataat aagcagtatc atccttggta tggtccttgt gtattacttc aatccccata
     6361 attccattag ttattaattc tgaaatgata ctgtcatctt caagtaagcc gggatgagca
     6421 agtaccggta tgcccctgca ttcttttatt agttttatag catcaattgg tgtcaatttt
     6481 tttcttggca cataggcagg tcttccctta gctaaaaact tatcaaaagc ctctttaatt
     6541 gaacttacat aacccttttc gactagagct aaagctacat gcggtcgtcc tacggaagtg
     6601 ccccgtgagt atttatagac atcatcatat tttatctgta tatcaatatc gtttaacttt
     6661 gtaaggattt tttccaatct ctttattctg ttttgctgta agtctaaaag ctgttctttt
     6721 aaatgaaaat tttcgtaatc aatatagtat cccaatatgt gaacttcctg gccattgcaa
     6781 tgtgtgctca attcgattcc gggaattacc tctagatcct tatacttatt ggcctcaatt
     6841 atagcaggta aaattccatc tacagtatcg tggtctgtga ttgcaattgc tttcaagttc
     6901 agttcatatg cctttttgac cagctgctgg ggagtaaata gaccatctga acaagttgtg
     6961 tgaagatgaa ggtctacttt cattacttat caccacattt atttcatcac ttcaattatt
     7021 atgagcaaaa aataagaatt ccaacctaca acaaggtgcc ttccgttccg aaaaccttgc
     7081 cttttgctaa agaactacgc tcgacacaaa acatgattat attttgtgtc acgaaattct
     7141 gtgattcaga aaaagcacac ccaataaata gacggcataa atgcgatatg ccgtcatctc
     7201 gaaaatcgaa aaaatacaat aatttcatat ataaaacaac tagaatatct ttttaacgcg
     7261 gctctacaat caacttaata gctgttcttt cctgaccgtc aatttcaata tcggtaaaag
     7321 ccggaataca aatcaagtca actccacttg gggcaacaaa tcctctcgca atagcaacgg
     7381 ccttaactgc ttgatttagt gctccggcac caaccgcttg aatttcggct gcaccacgct
     7441 cccttaaaac gcccgccaag gctcctgcca ccgaattcgg attggatttt gctgatacct
     7501 ttaaaacttc cattgtgtaa ttcctccctg cattttttgc ttcttaagta atttattcta
     7561 tattatattg gaaaatcctt gttttttgaa aaaaaattaa aaaaaatttt tagtttccca
     7621 ttgtctcttt tattct
//
LOCUS       DAONUO010000063         7607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7314_length_7607_cov_4.033898, whole genome shotgun sequence.
ACCESSION   DAONUO010000063 DAONUO010000000
VERSION     DAONUO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7314_length_7607_cov_4.033898"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /gene="recO"
                     /locus_tag="PK768_03510"
     CDS             <1..790
                     /gene="recO"
                     /locus_tag="PK768_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQA60067.1"
                     /translation="FIGFTQVSMALYKTDAIVLGHRNLGEADKILTLFSPDRGKIHAV
                     ARGVRRPRNPILAGSQLFSYSRFLIVEGRNLDSISQCEIKESFYRIRQNLESMAYGLY
                     FAELLRASTPIEDKNRELFRFFLKTLYLLQEWENKEVLARIYEVKLMAIQGFAPEVFS
                     CVSCGQSPSSSIYFSPSMGGILCFNCHKTDENAVSISSETLVAFQKMLMQTYEKLKNI
                     NISKYTREQLSNILSLFISHQIDRKLKTVQFIKDIKRLNIISSV"
     gene            822..1433
                     /locus_tag="PK768_03515"
     CDS             822..1433
                     /locus_tag="PK768_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HQA60068.1"
                     /translation="METFKGKGKLFLVSVTIFLAGLLTGYFIFLNDPGFVISNIEKLM
                     GNILRIGEAMKNRSKLHVTGLIFQNNIRALLVIMFGGIAFGLVSAFSIFFNGLIMGIV
                     MALTFYQGKTISFFLAGILPHGLLELPVVLGAGAFGLKTGLDLLFPKGKRRLDLLKDN
                     LNNNVLALGVFVPLLFIAAGIEAVITPFIINLLVGERGIFYNL"
     gene            1502..2110
                     /locus_tag="PK768_03520"
     CDS             1502..2110
                     /locus_tag="PK768_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA60069.1"
                     /translation="MIVDIVRKYEPITSEQIADKLNLTRSALRPDLAVLTMIGILEAR
                     PRVGYFFTGKIPQNLISKRIKDIKVDNIKAVPVVITENTSVYDAIVTLFLTDVGTLFV
                     TGDDGFLSGLVSRKDLLKIAIGNADIHKMPVGIVMTRMPNIITVTPDESAYEAAKKIV
                     DHQVDALPVVHVVVEDNQEKFKIVGKVTKSTFTKLFVELVEI"
     gene            2121..2951
                     /locus_tag="PK768_03525"
     CDS             2121..2951
                     /locus_tag="PK768_03525"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HQA60070.1"
                     /translation="MMVKDKSSHPIVFAVSDSIGETAELVTRATMVQFNSENVEIRRI
                     PYVTNNEYVEEIIQEAKNTSSCAIVCTIILPDVREYLIHLAEENNIPIVDLMGPMMKV
                     FSEIVEIKPHLEPGLVRRIDEDYFKRMEAIEFAVNYDDGKDPRGLSRADVVLIGVSRT
                     SKTPLAMFLAHKRLKVANLRLVPEVAPPEELFNLPPNTVVGLTISPEKLFDIRQERLK
                     AMGLSADANYAKHSRILEEIEYANKIMEKIGCPKIDVSNKAVEEIATHIMEIIKKGEK
                     "
     gene            2953..5604
                     /gene="ppdK"
                     /locus_tag="PK768_03530"
     CDS             2953..5604
                     /gene="ppdK"
                     /locus_tag="PK768_03530"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQA60071.1"
                     /translation="MTKKYVYSFREGNATMRSLLGGKGANLAEMVKIGLPVPMGFTVT
                     TEACLNYYDEGEKISEDIVSQIFGALENLEQEMGKKLGDIQDPLLVSVRSGAVISMPG
                     MMDTVLNLGLNDESVKGLAAKTDNERFAYDSYRRFIQMFGNVVMEVDHGNFEAVIESV
                     KEKKNITEDTEMDAEGWKEVISLYKEKIKEETGREFPQDPKEQLLMAVEAVFKSWNNQ
                     RAKVYRRINKIPDDLGTAVNVQTMVFGNKGDDSGTGVAFTRNPSTGENKVYGEFLMNA
                     QGEDVVAGIRTPQSIDQLKEVMPDIYKQFTDVCTLLENHYKDMQDIEFTIENGKLYIL
                     QTRNGKRTAQAAVKIAVDMVHEGLISKEQAIIQVPAGQLETLLHRRIDPNAKVEAVAK
                     GLPASPGAASGSVVFDPDEAEVMGNDGKKVILVRTETTPDDIHGIVAAQGVLTSRGGM
                     TSHAAVVARGMGKPCVSGCEAIRIDYEKEEFQVGDTVVKKGDIISIDGSTGQVIIGEV
                     PMVEPELSDEFNELLSWADEARTLKVRANADTPRDAKTALEFGAEGIGLCRTEHMFMA
                     QDRLPIVQQMIMSETKEEREEALAKLLPIQQDDFEGIFEVMDGLPVTVRLLDPPLHEF
                     LPNAEELIEEIHELKANGNSEELEAKREMLKKVRALSEMNPMLGLRGCRLGLLYPEIY
                     IMQVKAILKAASKLTQRGIKVIPEIEIPLVGHELELENLRAIVEETAEKVLEEEGIKV
                     DYFIGTMIELPRACLTAEDIAKHADFFSFGTNDLTQTTFGFSRDDAEGKILPHYIEQK
                     ILTDSPFIILDRKGVGSLMKMAVEKGRQEKEDLLIGICGEHGGEPSSVEFCHMIGLDF
                     VSCSPFRVPIARLAAAQAAINHKDQTK"
     gene            5842..6330
                     /locus_tag="PK768_03535"
     CDS             5842..6330
                     /locus_tag="PK768_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60072.1"
                     /translation="MTSLILYLATAILYVFSYSKDRDKTRQAFLKGWKSLTNMMPQFL
                     GIIIVVGVTLALLKPETISSLLGSNSGAFGVVLSAIIGSIVMMPTFVAFSTGSMLLNT
                     GAGLPQVAALISTLTLIGVVTIPMEAKYIGRKATLYRNLVAFFFSILVGFVIEKVVEI
                     LW"
     gene            6324..6854
                     /locus_tag="PK768_03540"
     CDS             6324..6854
                     /locus_tag="PK768_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQA60073.1"
                     /translation="MVKSLVKKYSFFLIVFFMTAVLTLINKEVGFKSFGIAFSSFKQM
                     VSLVPPIMLLLGLMDVWVPRETMMKYMGAKSGITGIILAILMGTLAAGPMYAAFPFTT
                     VLLRKGVKFSNIIIFMNAWCVTKISTLLFELASLGFEFTFARLLINLPGVIIMGYIVE
                     ALMPKSELDSLYANNS"
     gene            7037..>7607
                     /gene="dgt"
                     /locus_tag="PK768_03545"
     CDS             7037..>7607
                     /gene="dgt"
                     /locus_tag="PK768_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HQA60074.1"
                     /translation="MNIRERQEELERIYLSPYAALSSKSKGRRIYEEKCSVRTDFQRD
                     RDRILHSKAFRRLKHKTQVFISPEGDHYRTRLTHTLEVSQIARTISKALRLNEDLTEA
                     IALGHDLGHTPFGHTGEQVLDELMKEGFRHEEQSLRVVEVLEQGKGLNLTWEVRDGIR
                     NHTTRGNPATLEGQVVKLSDWIAYINHDID"
BASE COUNT         2347 a         1361 c         1797 g         2102 t
ORIGIN      
        1 ttttatcggc ttcacccaag tttctatggc tttatataaa accgatgcta tagttttagg
       61 ccatagaaac ttgggtgaag ccgataaaat tctcaccctc ttttcacctg acagggggaa
      121 gattcatgca gttgcaagag gtgttcgcag gcccaggaat cctatattgg ccggaagtca
      181 attgttctct tatagtagat ttttaatagt tgaaggcaga aacctggaca gtattagtca
      241 gtgcgagata aaggagtcct tttacaggat acgccagaat ctagagagta tggcttacgg
      301 tctttatttt gccgaacttc ttagggcttc aacacccatt gaagataaga accgagaatt
      361 atttcgcttt tttttaaaaa cactgtattt gctccaggaa tgggagaata aggaggtatt
      421 ggcccgcatc tatgaagtaa aactaatggc aatacagggc tttgcaccgg aagttttttc
      481 ctgtgttagc tgcggtcaga gcccttcaag ttcaatatac tttagtccat ccatgggtgg
      541 aattttgtgc tttaattgcc ataaaacaga tgaaaatgct gtaagtataa gttcggaaac
      601 ccttgttgcc tttcaaaaaa tgttaatgca aacttacgag aaactcaaaa atattaatat
      661 aagtaaatat acaagagagc aactgagcaa cattttaagc ctttttatat cacatcaaat
      721 tgaccgtaaa ctaaaaactg ttcaattcat aaaagacatt aagaggctaa atataataag
      781 cagcgtataa tcgaacgggg gtgagcctgc tggataagat cttggagact ttcaaaggta
      841 aaggaaaatt gtttttggta tctgttacaa tatttttagc tggcctgctg acaggatact
      901 tcattttttt aaacgatcca ggatttgtga tatcaaacat tgaaaaactt atgggcaata
      961 tcttaagaat aggtgaagct atgaaaaacc gtagcaagct tcacgtaacc ggcttaatat
     1021 tccagaacaa tataagggct ctcctcgtaa tcatgtttgg cggaatagca ttcggattgg
     1081 tctctgcatt tagtattttt ttcaatggtt tgattatggg catagtaatg gctttgacct
     1141 tttatcaagg caagacaata tcgttttttt tggcaggtat attaccgcat ggattgctgg
     1201 aattacctgt cgtccttgga gcaggagcct ttggcttaaa aaccggctta gacctgctgt
     1261 ttccaaaggg caagaggcga cttgacctgt taaaagacaa tttaaacaat aacgtccttg
     1321 cattgggtgt atttgtgcct cttcttttta ttgcagcagg tatagaagcg gttataacgc
     1381 cctttataat aaatttatta gttggagaaa gaggaatttt ttataatttg tagtaatata
     1441 tattttggcg attcgggtat gaagggagtg gtaccccatc gagttatcgt cacgacaggc
     1501 aatgatagta gatattgtta gaaaatatga acctatcacc agtgaacaga tagcggacaa
     1561 gcttaactta accaggtcgg cgttaaggcc ggatcttgcc gtcttaacta tgataggcat
     1621 tttagaagcc agacccaggg ttgggtactt ttttaccggc aagattcccc aaaaccttat
     1681 ttcaaaacga attaaggaca taaaagtgga caatataaag gcggtccccg ttgttataac
     1741 tgaaaatact tccgtatatg atgctattgt gacgctgttt ttaacggatg taggaactct
     1801 ttttgttaca ggtgatgatg gatttctatc cggccttgtt tcacgtaagg acttgctcaa
     1861 aatagctatc ggcaatgcag atattcataa gatgccagtt ggtatagtta tgactagaat
     1921 gccgaacatc ataactgtta cacctgatga aagtgcatac gaggctgcaa aaaagattgt
     1981 tgatcatcag gttgatgcat tgcccgtagt tcatgtagta gttgaagata atcaggaaaa
     2041 gtttaaaata gtaggtaaag tcactaagag cacttttaca aaactgtttg ttgaattggt
     2101 tgagatttag agaggggagt atgatggtaa aggataaaag tagtcacccg attgtatttg
     2161 ctgtttccga ttctatcgga gaaactgcag aattagtaac tcgagcgaca atggtccagt
     2221 ttaattctga aaatgtggag attcgcagaa ttccttacgt tactaataat gaatacgtgg
     2281 aagaaattat ccaggaagca aaaaacacga gtagctgtgc tatcgtgtgt accattatct
     2341 tgcctgacgt aagagaatat ctgatacatt tagcggagga aaacaatatt ccaatagtgg
     2401 atttaatggg acctatgatg aaggtttttt cagaaattgt cgagattaag ccccatctcg
     2461 agccaggcct ggttcgtcgc atagatgaag actattttaa aaggatggaa gcaatcgaat
     2521 ttgccgtcaa ctatgatgat ggcaaggatc ccaggggatt gtcccgagcc gatgtggtcc
     2581 ttataggtgt atccagaacc tcaaaaacac ctttagcaat gtttttggcc cacaagaggt
     2641 tgaaggttgc taatcttcga ttagttccgg aggtagctcc accggaagaa ttgtttaatc
     2701 tccctccaaa taccgtagtt gggctgacaa tcagtcctga aaaacttttt gatattcgtc
     2761 aggaaaggct taaggcgatg ggcttaagtg ccgatgcaaa ctatgccaag cacagtagga
     2821 ttttagaaga aattgaatat gccaacaaga ttatggaaaa aataggttgc cccaaaatag
     2881 atgtttcaaa taaagcggtg gaggaaattg caactcatat tatggaaatt ataaagaaag
     2941 gagaaaaata gtatgacaaa aaaatatgtc tacagcttta gggaaggcaa tgccacaatg
     3001 cgcagcttat tgggaggtaa aggcgccaat ttggctgaaa tggtaaaaat cggcttaccc
     3061 gttcccatgg gctttacagt aacaaccgaa gcctgtctga actactatga tgagggagaa
     3121 aaaatatcag aagatatcgt ttctcagatt tttggggctc tggagaacct agaacaggaa
     3181 atggggaaaa aactcggtga cattcaggat cccctgttgg tttccgttag gtcaggagcc
     3241 gtaatatcca tgcctggcat gatggacact gtattaaacc taggtctcaa cgatgaaagc
     3301 gtgaagggcc tagctgcaaa gaccgacaat gagcgttttg cttacgacag ctaccgcagg
     3361 tttattcaga tgtttggaaa tgtagtcatg gaagtggatc atgggaattt cgaagcagtt
     3421 atcgaatcag taaaggaaaa gaaaaacatt actgaagata cagaaatgga cgcagaaggc
     3481 tggaaggaag ttatatccct atacaaggaa aaaataaaag aggaaaccgg cagagagttt
     3541 ccccaggatc ccaaggaaca gcttttaatg gcggtagaag ctgtatttaa gtcttggaat
     3601 aatcagagag ccaaggtgta taggagaatt aacaagatac cggatgattt agggactgca
     3661 gtaaacgtcc agaccatggt atttggtaat aaaggcgacg attcaggaac cggagtcgca
     3721 tttacccgga acccctctac aggagaaaac aaagtttacg gtgagttcct gatgaacgct
     3781 caaggggaag acgtagttgc aggtatcagg acaccccaga gtatagacca gttaaaagag
     3841 gtcatgcccg atatatacaa gcaatttaca gatgtctgca ctttattgga gaatcattac
     3901 aaggacatgc aagacataga gtttaccata gaaaatggga aactatatat cctccagacc
     3961 cgcaacggaa agcggacggc tcaggctgcg gtaaagattg ctgtcgacat ggtacatgag
     4021 ggtcttatca gcaaagaaca ggccatcatt caggtgcccg cgggacaatt agaaactctg
     4081 cttcatcgca ggatcgaccc caatgctaaa gttgaagctg tagcaaaggg actgccggct
     4141 tcgcccggag cggcttcagg cagcgtagtt ttcgatcccg atgaggccga agttatgggc
     4201 aatgatggca agaaggttat tttggtcaga actgagacca ctcccgatga catacacggt
     4261 atcgtagctg cccagggtgt acttacaagc cgcggcggca tgacaagcca cgctgctgta
     4321 gttgccagag gcatgggcaa gccttgcgtg tctggatgtg aagcaataag aatcgactat
     4381 gaaaaagaag agtttcaggt tggagatact gtagttaaga agggcgacat aatttcgata
     4441 gatggttcta ctggccaagt cattataggt gaagtcccga tggtagagcc ggagctttct
     4501 gatgagttta atgagctgct tagctgggca gatgaagcga gaacacttaa ggtaagagca
     4561 aatgcagaca ccccacgtga tgcaaagaca gccctcgagt ttggagcaga aggtattggt
     4621 ctttgcagaa ccgagcacat gttcatggcg caggacagac tgccgatcgt tcaacagatg
     4681 atcatgtcag aaaccaagga agaaagggaa gaggcgctgg caaagctttt acccatccaa
     4741 caggatgatt tcgaaggtat ttttgaagtg atggacggct tgccggtaac ggttagactc
     4801 cttgaccccc ctctccatga attcctgccc aacgctgagg aattaatcga agaaatccat
     4861 gaattaaaag ccaatggcaa ctccgaggaa cttgaagcaa aacgggaaat gctgaaaaaa
     4921 gtgcgtgcac ttagtgaaat gaatcccatg ctgggtctaa ggggttgcag actgggcttg
     4981 ttatatccag aaatttacat tatgcaagtc aaggcaatat tgaaggcagc aagcaaactc
     5041 acccaaagag gcattaaggt aatacctgaa atagagatac ctttggtagg acatgagctg
     5101 gagcttgaga atctcagggc aatagtggaa gagacagccg aaaaggtatt agaagaagaa
     5161 ggaataaagg tcgattattt cattgggacc atgattgagt tacccagggc atgtctaact
     5221 gctgaagaca ttgcaaaaca tgcggacttc ttctccttcg gaacaaacga tttgacacag
     5281 accacctttg gctttagccg tgacgatgca gaaggcaaga tactgcccca ttatatagag
     5341 caaaagattc tcaccgactc accgtttatc atccttgata gaaaaggtgt gggctcttta
     5401 atgaagatgg cagtagagaa aggccgtcaa gaaaaagaag atctattaat aggtatttgc
     5461 ggtgaacatg gtggagaacc aagctctgtt gagttctgcc atatgatcgg gttggatttt
     5521 gtcagttgct caccattcag ggtgccgatt gcgagacttg cagcagcgca ggcggcaatt
     5581 aatcacaagg accaaactaa gtaagtgtaa ataaaaataa taataagggt actgttacta
     5641 ataggtgcag tacccttttt tgtgaatttg tatagtatca atctatctaa ataagtccaa
     5701 agactacctt agccaatagg aagcgcttat aggttattgc ataccttatt ccactgcttg
     5761 tccatctgct tgtattgaca gagccatctt atgaggaata taatatggat gtaatggaga
     5821 aaacagttta ggagtagtag tatgactagc ctaattcttt accttgcaac agcgattttg
     5881 tatgtttttt cgtatagcaa agatagggac aagacgagac aagcgttttt aaagggatgg
     5941 aagtcattga caaacatgat gcctcaattt ctgggcatta ttattgttgt tggagtgacc
     6001 ctagctttgc ttaaacctga aacgatttct agtttattgg gaagcaattc cggtgctttc
     6061 ggggtagtat tgtctgccat tatcggaagc attgtaatga tgccaacctt cgtagctttt
     6121 tccactggat ctatgttact aaatactggt gctggccttc cgcaagtagc tgctctcata
     6181 tcaacgctga cactaatagg tgtcgtaacc attccaatgg aagcaaaata catagggaga
     6241 aaggcaacct tgtatagaaa tttagtggct ttcttttttt caattttagt tggtttcgtc
     6301 attgaaaagg tggtagaaat cttatggtaa aatctttagt gaagaagtat tctttttttt
     6361 tgattgtatt ttttatgacc gcggttttaa ctttaataaa taaggaagta ggatttaagt
     6421 ctttcggaat agccttttcc agttttaagc agatggtctc tttagtacca cctataatgt
     6481 tgttgcttgg tctcatggat gtttgggtcc ccagggaaac tatgatgaaa tacatgggag
     6541 ctaaatctgg gataacaggg attattcttg ccatactgat gggtaccctt gcagctggcc
     6601 ccatgtacgc tgcttttcct tttacgacgg tgcttttgag gaagggtgtt aaattcagca
     6661 atataatcat ttttatgaat gcatggtgtg taacaaaaat atccaccctg ctttttgagt
     6721 tagcgtcact gggctttgag tttacctttg ctaggctttt gattaacctt cccggcgtga
     6781 taataatggg ctacattgtg gaagctttaa tgccaaaaag cgagttggat agcttgtatg
     6841 caaataattc ataagtgaaa cgcagatttc ctgtttctat atagtatcag attgcattgt
     6901 tagacaggac gcaatttcag tattatattt taagaggcga tttttcgcct ctttttgtta
     6961 tatttaaagg aaaaaatagc ttggaagtag aatatcttta tgatattttc cgatgccttt
     7021 tgggaggtga tttcaggtga atatccgtga aaggcaggaa gagttggaac gaatatatct
     7081 ttcgccatat gcagctctaa gctcaaaaag caaaggacgg cgtatatatg aagaaaaatg
     7141 cagtgttcgc actgattttc aaagggaccg ggaccgaata cttcattcaa aagcttttcg
     7201 gcgattgaaa cacaaaaccc aggtgtttat atctccggag ggggatcatt acagaaccag
     7261 acttacacat acactggaag tgtcacagat agcaaggacc atatctaagg ccttgagatt
     7321 gaatgaagac ttaacggaag ctattgcttt gggccacgac ctgggtcaca cgccctttgg
     7381 ccataccggc gaacaagtat tagatgaatt aatgaaagaa ggctttagac acgaagaaca
     7441 gagcctcagg gtcgttgagg ttttggaaca gggcaagggg ctgaatctta cctgggaggt
     7501 cagggacgga ataagaaacc atactaccag gggtaatcct gctactctcg aaggccaggt
     7561 ggttaaactg tccgattgga tcgcatacat taatcatgat attgatg
//
LOCUS       DAONUO010000064         7592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7334_length_7592_cov_3.822874, whole genome shotgun sequence.
ACCESSION   DAONUO010000064 DAONUO010000000
VERSION     DAONUO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7334_length_7592_cov_3.822874"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PK768_03550"
     CDS             complement(<1..788)
                     /locus_tag="PK768_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HQA60075.1"
                     /translation="MEDQKEKRLINWAIEKTRSEVSKAETKKDISSKESDFDENATIE
                     IELSNNEMTATLSLIPPKGGKMVSIEEIMEELQSKGIEYGINKDKISEIISHRIFNTP
                     IEIATGLPPKDGEDGKINYFFDTKKSLKPKILEDGTVDHYNLGLVINVTKGQQLAEVI
                     PPSKGIPGMTVTGKTVKARDGREARIRVGKNVVISEDGLKAFADIDGQPVLYNDKLSV
                     LPILEIKGDVGPATGNIDFLGSVIVMGNVKSGFAIKASHDLEVYG"
     gene            complement(808..1545)
                     /locus_tag="PK768_03555"
     CDS             complement(808..1545)
                     /locus_tag="PK768_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HQA60076.1"
                     /translation="MLNANTENLWKIYHTSKDHSVKEKIIENYLPLIKHIVNRMNVKL
                     PSYLEYDDLISYGVFGLLQAIERYDAKKGIKFETYAYSRIKGSIIDELRKIDWIPPGV
                     RKKAKILQNAFAELEQSLGRSVNDEDICKHLNISVDELHKMYSDVATLYNVLTFDDLI
                     NIETFKSDSQPEIQAEKQELKEILGNAINKLSPQERLVITLYYYEGLNFKEIANVLDL
                     SQGRISQLHTKAILRLRGHLSRKKTHL"
     gene            complement(1557..2045)
                     /locus_tag="PK768_03560"
     CDS             complement(1557..2045)
                     /locus_tag="PK768_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HQA60077.1"
                     /translation="MNTIIRVGMADYKAAKSPAILITLGLGSCVGVVLYDKIKKVGGL
                     AHVMLPDSSLSNQKDFNPGKFADTAIDALIEDMLEFGAIRNNIVSKIAGGAQMFKIKS
                     DNNIMQIGKRNVEAVRSKLKELNIRVLAEDVEGNYGRTIEFFCETGELTVKTIGHGVR
                     IL"
     gene            complement(2008..2649)
                     /locus_tag="PK768_03565"
     CDS             complement(2008..2649)
                     /locus_tag="PK768_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HQA60078.1"
                     /translation="MKYTDLEIDALKEIGNIGAGNAATALSMMLSKKITIKVPQVKII
                     PFDEVSDSVGGPEKLVVGIFLRISGDIDGNILIVISEKDAYTLAKTLLNYNRDINRDF
                     SDLEESALNELGNIIGSSYVTALSELTNLSLRISVPSMAFDMAGAIISFPLSLYGYLG
                     DTAFLIETEFVEGLAGIKLNYFMIPDDKSLKRLLKAIGVSTIEHNNSCRNGGL"
     gene            complement(2660..3115)
                     /locus_tag="PK768_03570"
     CDS             complement(2660..3115)
                     /locus_tag="PK768_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQA60079.1"
                     /translation="MADSIRQFVEFKLGQEEYGIDILQVKTIERMMPITRVPKAPRFV
                     EGVINLRGEIVPVIDLRKRFDLPITETTENTRIIIVSVDDITVGMIVDSATEVVQLDQ
                     DSIEPAPPITSSIDSDYLDGVGKLDDKLLILLNVEKLLKPQEIDELAQI"
     gene            complement(3129..5123)
                     /locus_tag="PK768_03575"
     CDS             complement(3129..5123)
                     /locus_tag="PK768_03575"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HQA60080.1"
                     /translation="MNNQYLDVFLEEAKEHIENLNKYLLELENNPSQEIVDEIFRSAH
                     TLKGMSGTMGFTQLLELTHEMENLLQEIRSQEISVNEHIIDTLLKSVDILEELIHDVE
                     EHGQEQERDIHMVLQMLKGKKAIGLDEAKVTPNENEENYVFSFNEYEERIIRKALEQG
                     LYIWRVKVTLVDECLLKSARAFLVFKNLEAFGDIIKTEPSVQDIEDEKFDNEFTLYLI
                     TSKNKNDISQALSSVSELKSVDVTSVEHNSIAVRKIESDKEQNSLETVKTLASRQKTS
                     KGTNGVRRTSKTLRVDIERLDNLMNLVSELIIIKTRLEDIKDNNYQAQRETIEYLERI
                     TSNLHDAVMKARMVPIENVFNRFPRVVHDLSRELSKKINLIIEGAETELDRTVIDEIG
                     DPLIHLIRNAADHGIEYPQERVKKGKPEHGTINLKAYQDGNNVIIEVSDDGRGIDLSS
                     VLTRALDVGLTSAEQAKNLKDYEIISYLFEPGFSTSSEVTDVSGRGVGLDVVKTKIES
                     LGGSIETSFIKDKGTTFQIRLPLTLAIIQALMVLVGNEKYAIPLSSIRETVIVTKDQI
                     KTVQKREVILLRGNVIPIIRLNQALETEKFDENQKELTLVIVKKGEKNVGLAVDDLIG
                     QQEIVIKSLGKFLNDIEFIAGATILGNGEVALILDINKLV"
     gene            complement(5142..5783)
                     /locus_tag="PK768_03580"
     CDS             complement(5142..5783)
                     /locus_tag="PK768_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQA60081.1"
                     /translation="MFEVILEIGMKVIIEVKGDSGDLYFSSKIEDIGSDDITLGMPMK
                     GGRTFFIAVDENINIYFSRKGSFYCIEGKVLEKRYRPIPVIKILPLSSPYKKQKRSFF
                     RLKISLPIHVKMDDAEGSFIRYTRDISAGGIKFSHSDLIEVGTNLEITIPDVLGNKTA
                     IKAIVRRAEYDSTREINKYDIAIEFLDIDDRTRDIIVKFILVKQRELRKNGLE"
     gene            complement(5783..6670)
                     /locus_tag="PK768_03585"
     CDS             complement(5783..6670)
                     /locus_tag="PK768_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HQA60082.1"
                     /translation="MYEQALKLKKLMEQKKPPTEFDKKVKVYCVASGKGGVGKTNLSV
                     NLSLVLQNLGKNVLLIDGDLGLANIDVVTGLYPKYNLSHILTAGKSIQDVILDGPKGI
                     SILPGASGLYELANISSSELEILIKSFNSISSIFDIIIIDTGAGISKNVLSFIQSSHE
                     VIVVTTPEPSAVTDAYALIKLAYKHCDTINVIINKADNYKEASFTMGKLAKAAKKFLN
                     IDIRYLGFVLEDKTVLKANMEQTPFLIKYPNGLASKCLINITNKLLYGQEIHNEGTVT
                     IEGWFKKLMSFIKTNVGAL"
     gene            complement(6663..>7592)
                     /locus_tag="PK768_03590"
     CDS             complement(6663..>7592)
                     /locus_tag="PK768_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQA60083.1"
                     /translation="KNNQATSNTGNDYLKTEIKEVKKLLNEIYIDTKVQQGNSNALDV
                     NLPLSIKKYYDRMHEMEVDPTIINKLINNTTKALSDTELNDKAILYKTLKKEIKSFVD
                     DIEPIELSNKNYIVAFVGPTGVGKTTTIAKLATHFSLYKNKKVALATADTFRVGAVEQ
                     LKHYGDLLEIPVHVIHRQEDVRETLASLSDYDLLLVDTMGFNPNNRMQIKKIKGLLDV
                     LNPDDVHVVISAATKNRDLTDILNNYKELYYNKILITKFDETKTYGVVLNALNVGKCK
                     LSYFTMGQNVPDDIELPSADKIAKMILGEKEDV"
BASE COUNT         2098 a         1575 c         1136 g         2783 t
ORIGIN      
        1 ccgtaaactt ctaaatcatg acttgctttt atagcaaaac cgctttttac atttcccatt
       61 acaattacac tgccaagaaa gtcaatattt cctgttgcag gacccacatc cccttttatt
      121 tcaagaatag gcaaaacgct gagtttgtca ttatacaata ccggttgtcc atcgatatct
      181 gcaaaagctt ttaagccatc ttcagaaatt accacatttt tgccaacacg aattcttgct
      241 tccctaccgt cccttgcttt tacagtttta cctgtaacgg tcataccggg aatgcccttg
      301 ctgggtggaa tgacttcggc aagctgctgc ccttttgtta cattgatgac taatcctaaa
      361 ttatagtggt cgacagtccc gtcttccagt attttaggct taaggctttt tttagtatca
      421 aaaaaataat ttatcttacc atcttcaccg tctttcggag gcaggcctgt agctatttca
      481 ataggcgtat taaatatccg gtgacttatt atttcactaa ttttgtcttt atttataccg
      541 tactcaattc cttttgattg aagttcttcc attatttctt caatggaaac cattttgcca
      601 cctttaggtg gaatcaggga aagggtcgct gtcatttcat tgttgctcaa ttcaatctca
      661 attgtggcat tttcatcaaa atctgattct tttgaactaa tgtctttttt tgtttcagct
      721 ttgcttacct cggacctagt tttttcaatt gcccagttaa taagtctttt ttctttctga
      781 tcttccacct gatcacctct ttcattttta tagatgcgtt ttttttctgc ttaaatgccc
      841 cctcaatctc aatatcgctt tagtgtgaag ctgagaaatg cgtccctgtg aaagatctaa
      901 aacattagct atctctttaa aattcaggcc ttcataatag tataaagtta ttaccagcct
      961 ttcttgcggc gaaagcttat tgatggcatt gcccagaatt tcttttagct cctgtttttc
     1021 agcttgaatc tcaggttgac tatcggactt aaacgtttct atatttatta aatcgtcaaa
     1081 agttaaaaca ttatacaaag ttgctacatc agaatacatt ttgtgcagtt catcaacaga
     1141 aatattaagg tgcttacaaa tatcttcgtc atttactgat cgccctaaac tttgctctaa
     1201 ctctgcaaaa gcattttgaa gtatttttgc tttctttctc actccaggtg gaatccaatc
     1261 tatttttctc agttcatcta taatagaacc cttgattcgg gaataagcat atgtttcaaa
     1321 tttaattccc tttttagcat cgtacctttc gatggcctgc agtaagccga atactccgta
     1381 acttattaaa tcatcatatt ctaggtatga tggcaacttt acattcattc ggtttacaat
     1441 gtgtttgatt agaggtaaat aattttcaat aattttttct tttacagaat ggtctttgga
     1501 agtatgataa atcttccaca agttctcggt attcgcatta agcatgatgc acctcattac
     1561 aaaatcctga caccatgacc aattgtctta actgtaagtt ctccggtttc acaaaagaat
     1621 tctatagtcc ttccataatt cccttctaca tcttcggcaa gaacccttat gtttaattct
     1681 ttaagcttcg atctgacagc ctccacattt cttttaccga tctgcataat attgttatcg
     1741 gattttattt tgaacatttg tgcacctcca gcgattttgc tgacaatatt gtttctaata
     1801 gccccaaatt caagcatatc ttctatcaag gcatcgatag cagtatctgc aaatttacct
     1861 ggattaaaat ctttttgatt gctgagactg ctgtctggaa gcattacgtg agccaagcca
     1921 cctacttttt ttatcttatc gtataagact actcccacgc acgagcccaa acccaatgtt
     1981 atcaagatag caggagactt tgctgcctta taatccgcca ttcctacacg aattattgtg
     2041 ttcaatagtg ctcactccaa tagcctttaa aagtcgcttt aatgatttat cgtcgggaat
     2101 cataaaataa ttcagtttaa tacccgctaa gccttcaaca aactctgtct caattaaaaa
     2161 tgccgtatca cctaaatacc cgtacagact tagaggaaag ctgattattg cccctgccat
     2221 atcaaaggcc atactgggaa cagatatcct tagggaaaga ttggtaagct ctgaaagtgc
     2281 ggtaacatat gaacttccga taatgttgcc taattcattt aaagctgatt cttccaaatc
     2341 ggagaaatcg cgatttatgt ctctgttata attcagcagg gtttttgcaa gggtatatgc
     2401 atccttttct gatatgacaa ttaagatatt cccgtcgata tctccgctta ttcgcaggaa
     2461 gattccaact acaagttttt ccgggccgcc tactgaatca gaaacctcat cgaagggaat
     2521 tatttttacc tggggaactt ttatggttat tttttttgaa agcatcatgg acaatgctgt
     2581 agctgcattg cctgcgccaa tgtttccaat ctcttttagg gcatcgatct ccaaatccgt
     2641 atacttcata tattcaccct catatctgag ctaattcatc tatttcttgc ggttttaaga
     2701 gtttttctac attcagtagt atcaatagct tatcatctaa tttacctact ccgtcaagat
     2761 aatccgaatc gatgctgctc gtaatggggg gcgccggttc aatagaatcc tgatcaagct
     2821 gcactacttc tgtggcggag tcaacaatca ttccgacagt aatatcatca actgaaacga
     2881 ttataattct cgtattttcg gtggtttctg taatcggcaa atcaaatctt tttcttaaat
     2941 ctataactgg aactatttct ccccttaggt ttattactcc ttctacaaat cttggagctt
     3001 ttgggaccct ggtaattggc atcattctct ctattgtttt aacctgcaaa atatctatac
     3061 cgtactcttc ttgtcctaat ttaaattcca caaactgacg aatgctatcg gccattatta
     3121 atcctccttt agaccaattt atttatatca agtattaaag caacctcacc gttgccaaga
     3181 attgtagctc ctgcgatgaa ttcaatatca tttaagaatt tgcccaagct tttaattacg
     3241 atctcttgtt ggcctataag atcatccaca gccaaaccta catttttttc acctttttta
     3301 actattacta aagttaactc tttctgattc tcatcaaact tttctgtttc taatgcctgg
     3361 tttagtctta tgataggaat tacgttgcct cttagcaata ttacttccct tttttgaacc
     3421 gtttttattt ggtctttagt aactataaca gtctctctaa ttgaacttag aggaatagca
     3481 tatttttcat tgcctaccag caccattaat gcttgaatta tagcaagtgt aagggggagt
     3541 ctaatttgaa aggtcgtccc tttatccttt ataaagcttg tttcaataga accacctaat
     3601 gattcgattt tagtcttaac tacatccagg ccaacccccc ttcctgaaac atccgtcact
     3661 tcacttgatg tgctaaaacc gggttcgaat aaatatgata ttatttcata atcctttaag
     3721 tttttcgctt gttcggcact agtgagacct acatctaagg cccttgttag cacactactt
     3781 aagtcaattc ccctgccgtc atcggaaacc tcaatgatta cgttgtttcc gtcttgatag
     3841 gcctttagat taatagtccc atgttcgggt ttgccttttt ttactctttc ctgtgggtat
     3901 tctattccgt ggtctgcggc attgcgaata agatgtatga ggggatcccc aatttcatct
     3961 ataaccgtcc tatctagttc tgtttcggcc ccttcaataa tcaaattaat ctttttcgac
     4021 agttcacgag acaaatcgtg aacgactctt gggaatctat tgaatacatt ttctatgggc
     4081 accattcttg ccttcattac cgcatcgtga agatttgaag taattcgctc caaatattct
     4141 attgtttcac gctgcgcctg ataattattg tctttaatat cttctaatcg agtttttata
     4201 ataattaatt cactgaccaa attcattaag ttatccaatc gctctatatc cactctcaga
     4261 gttttgctcg ttctcctaac accatttgtc cctttagaag ttttttgcct agatgctaaa
     4321 gtttttacag tttcaagact attttgttct ttatctgatt caatttttct aacagctatt
     4381 gaattatgct caacacttgt aacatctact gatttgagtt cagatacaga acttaaagcc
     4441 tgtgaaatat catttttgtt ttttgatgta atcaaataca aagtaaattc attgtcaaat
     4501 ttttcatctt ctatatcctg tacggacggt tcagttttta taatgtcgcc aaaggcttct
     4561 aaatttttaa aaacgagaaa agctcttgca gattttaaaa ggcactcatc aacaagtgtc
     4621 acctttactc tccatatgta aagaccttgc tctaaagctt tacgaattat cctctcttca
     4681 tattcattaa aactaaaaac ataattttct tcgttttcat taggagttac ttttgcctca
     4741 tccaagccaa tagctttttt acctttgagc atttgtaata ccatatgtat atcgcgttct
     4801 tgttcctgac catgttcttc cacatcgtgg attaactctt ccaagatgtc aacgctcttt
     4861 aatagagtgt caataatgtg ctcattaaca ctgatttctt gacttcttat ctcttgtaaa
     4921 aggttttcca tctcatgggt caattccaag agttgagtaa agcccatggt acctgacatg
     4981 ccttttaggg tatgagcaga acgaaatatt tcgtcaacaa tttcctgaga agggttgttt
     5041 tctagttcta ataaatattt atttagattt tcaatatgct ctttagcttc ttctaaaaaa
     5101 acatctaaat actgattatt cattttattc acccctactt ttcattcaag accatttttt
     5161 ctaagttctc tttgcttgac aagtatgaat tttactatga tgtctcgtgt ccgatcatct
     5221 atatctaaaa attctatcgc aatatcgtat ttgtttattt cccttgtgct gtcatattcg
     5281 gctctccgca ctattgcttt aatagctgtc ttattcccca aaacatcagg aattgtaatc
     5341 tctaagtttg ttcctacttc tattaaatcg gaatgtgaaa atttaattcc acctgcgctg
     5401 atatcacggg tgtatcttat aaagctaccc tctgcatcat ccatttttac atgaatgggg
     5461 agtgatattt ttagtctaaa aaagcttcgc ttttgttttt tatacggaga actcaaagga
     5521 agtattttta taaccggaat gggtctatac cgtttttcta acactttacc ttctatgcaa
     5581 taaaatgaac cctttctgga aaaataaatg tttatattct cgtctactgc gatgaaaaaa
     5641 gttctgccac ccttcatggg catccccaat gtaatatcat cagatcctat atcttcaatt
     5701 ttagatgaga aatataagtc cccgctatct ccttttactt caattatcac cttcatgcct
     5761 atttctaaaa taacttcaaa catcatagag cccccacatt agttttaata aatgacatta
     5821 attttttgaa ccacccttca attgttaccg taccttcatt atgaatttct tggccataca
     5881 acagtttatt tgtaatgtta atcaagcatt tggaggccaa tccgtttgga tatttaatta
     5941 gaaaaggagt ttgttccata ttggccttta atacggtttt atcttccagg acaaaaccca
     6001 aatatctgat atctatattt aaaaactttt tcgctgcttt tgcaagtttt cccatggtaa
     6061 aactggcttc tttataatta tcagctttat ttattattac atttattgta tcacagtgct
     6121 tgtatgctaa ttttatcagg gcatacgcat ctgttactgc acttggttca ggtgtggtta
     6181 ctacaatcac ttcgtgagaa gactgaataa aactcaatac atttttagaa atccccgcac
     6241 ctgtatctat aattattata tcaaaaatac tcgaaatact gttgaagctt tttatcagta
     6301 tttccagctc gctactgcta atattggcaa gttcatacaa tcccgatgct ccgggaagta
     6361 tgctgatccc cttaggtcca tcgaggatta catcttgaat agatttaccg gcagttaaaa
     6421 tgtgagaaag attgtacttt ggataaagac cggttacaac atcaatattg gcaagaccca
     6481 aatccccatc tatcaaaagt acattctttc ctagattttg aagtaccaag ctcaaattaa
     6541 ccgacaaatt agtcttgcct acaccacctt tgccacttgc gacacaatag acttttactt
     6601 ttttatcaaa ttcagtaggt ggtttctttt gctccataag cttctttagc tttagagcct
     6661 gctcatacat cttctttttc ccccaagatc attttggcta ttttgtccgc cgaaggtaat
     6721 tcaatgtcat ctggaacatt ttggcccatt gtaaaatatg acaacttgca cttgccaaca
     6781 ttcagagcat ttaggactac gccataggtt tttgtttcat caaattttgt aatgagtatt
     6841 ttattgtaat ataattcttt ataattattt agtatatccg tcaaatcgcg attcttagta
     6901 gctgcactta tgacaacatg tacatcatca gggtttaata catctagcag gccttttatt
     6961 ttttttatct gcatgcgatt attgggatta aaacccatgg tatcaactaa caatagatca
     7021 tagtcactta aagacgctaa tgtttctcgc acatcttcct gtctatgaat tacatgaaca
     7081 ggaatctcta aaaggtctcc ataatgtttt aactgctcaa ctgcacctac tctaaatgtg
     7141 tctgcagttg ctagagccac ttttttgttt ttatacagac tgaaatgtgt cgcaagcttg
     7201 gctatggtag tagttttgcc aactcctgtg gggccaacaa aagctactat gtaatttttg
     7261 tttgacaact caatgggttc aatatcatca acgaatgact ttatttcttt ttttaaggtc
     7321 ttatataata tggctttatc atttaattct gtatcagaaa gtgctttagt tgtattatta
     7381 attagcttgt tgataatggt aggatcgact tccatttcat gcattctgtc ataatatttt
     7441 ttaatggaca agggcaaatt aacatctaaa gcattactat taccctgctg aacttttgtg
     7501 tcaatatata tttcattcag taatttcttg acttctttta tttcagtttt taggtaatcg
     7561 tttcctgtat tggatgtggc ttgattgttt tt
//
LOCUS       DAONUO010000065         7384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7577_length_7384_cov_4.402238, whole genome shotgun sequence.
ACCESSION   DAONUO010000065 DAONUO010000000
VERSION     DAONUO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7577_length_7384_cov_4.402238"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..690
                     /locus_tag="PK768_03595"
     CDS             40..690
                     /locus_tag="PK768_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQA60084.1"
                     /translation="MKTILCYGDSNTWGYNPDGSGRYPKHMRWTTVLQKELGESFDVI
                     SEGLNGRTTVWDDPVRGDYRNGKKYLLPCLHTHKPIDLVILFLGSNDLKPRYNVTSME
                     IAQSVEMLVDIVKKSETGPNMTSPEILVIIPPPILIPEEVKQMDYLLPEYEKAVDKSR
                     QFPQAFTSVLNGKCHLFDSSKYIKTSEIDGMHLDPESHAILGRELAKYIKDFLATP"
     gene            746..1597
                     /locus_tag="PK768_03600"
     CDS             746..1597
                     /locus_tag="PK768_03600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQA60085.1"
                     /translation="MYFKEIKTTASSTIKLFLGDCFEGMERYLEKNSVDVIVTSPPYN
                     IGVNYNKYDDKIPREEYLNWLGNWANITREVLKDDGSLFLNIGSKPSDPWVPFEVALE
                     MRKYFELQNVIHWIKSIYIEHESYGEKVELNVGHYKPINSKRFINDTHEYVFHLTKHG
                     NVELDRLSIGVPYKDESNITRWDSGRKGLRCRGNTWYVPYKTIQSRMKDRPHPASYPA
                     ELAKMCIQLHGVEKTKLVLDPFMGIGNTAIACKELDINCVGFEIDKVYFNEAIRILEE
                     GGQLCLI"
     gene            1585..2235
                     /locus_tag="PK768_03605"
     CDS             1585..2235
                     /locus_tag="PK768_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60086.1"
                     /translation="MPDLNLLAKEEKLEIIDGIKKAFRDVEFIGRFLDTFMLQINLKA
                     NPPSKVISSIFENNLPDFFSQISCAADLYTTLKDQHFQSAFDLAWESKMRKNKLRGHT
                     ISNIVSVCVESYLKNGRYFTDGDGSNDLKRVIDGSRWEIKGSRSKKFSLTINQSHVGL
                     DSTYFIVYNGFPEHNKIHGIYILKGDEHIFTPRQEGLNMRTFINEYYDTHVECIYP"
     gene            complement(2295..2960)
                     /locus_tag="PK768_03610"
     CDS             complement(2295..2960)
                     /locus_tag="PK768_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQA60087.1"
                     /translation="MCGSFLLMALYSDLIKYYSIDVEALWEYVPGDYFPNRNAPIVID
                     DGKRMLQFAKWGFPFSGKSRPVINARAETVTSKPMFKSSFFYRRCVIPANGFYEWKQE
                     ESGKKAKYRIGLRDEPLISLGGIYKISRDENSIEQVTFVIITTEANEQLKPVHHRMPL
                     IVRKEQLDLWLNNSTPQKHIMSILANDTYDGALVIEKETKEEASDSPKDSGGENPDQI
                     SLF"
     gene            complement(3058..3399)
                     /locus_tag="PK768_03615"
     CDS             complement(3058..3399)
                     /locus_tag="PK768_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60088.1"
                     /translation="MKNRFCVAIIIFCIILIGCSNNLSLEQASSSEENGETLDSSYTK
                     EEYEQGLEEYFDIENEEGIYARNIISWNTETGEEKGILKRKAAKRWNLKITSQVIPKK
                     IFSLSVKIVVK"
     gene            complement(3429..3626)
                     /locus_tag="PK768_03620"
     CDS             complement(3429..3626)
                     /locus_tag="PK768_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HQA60089.1"
                     /translation="MNLIIDRFEGDYAVVELPDRTFVNIPKKALPEEAKEGDIITITV
                     NKSETERRKDNIRKLADDLWE"
     gene            complement(3638..4669)
                     /locus_tag="PK768_03625"
     CDS             complement(3638..4669)
                     /locus_tag="PK768_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQA60090.1"
                     /translation="MNAKQLLACLLIILTVLLAACGSTALKEPVQTTEAETGLKVHFL
                     DVGQADCILIQAPTGENMLIDAGNNDDADTIISYLNAQGVRRIDVLVGTHPHEDHIGS
                     LDTVIDTYDIGKVYMPRVTHTTKTFEDVLIAIKNKGLKIAAAKAGEIIDFSPNTRVEI
                     LAPNSPEYDDLNNYSAVIKLTYGNTSFLFTGDAEDISENEMLASGYDLKADVLKLGHH
                     GSSTSSTEAFLAAVKPSYGVISVGKGNDYGHPHEEVLTRLEQAGIEIYRTDESGTVLF
                     ATDGDHIVIKTEKTGRTAKDVTVYITKTGKKYHMGDCNLLKDSRVPIKLSEAKARGYE
                     PCKSCKPPQ"
     gene            complement(5022..6062)
                     /locus_tag="PK768_03630"
     CDS             complement(5022..6062)
                     /locus_tag="PK768_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+-transporting methylmalonyl-CoA/oxaloacetate
                     decarboxylase subunit beta"
                     /protein_id="HQA60091.1"
                     /translation="MKVYYNSSLWSKGRGLWGWPQRVNWQFDYAGAKRYIPAIYRFSK
                     GIAFDVITILDEEKLKKFLERNEIAEGKELSPLQKRLIEQEHPCQDLAISRIWINGKQ
                     VLGGYSSSYRICIPWLEQEEALKPVRRAYSFILKDSTPFACQRYSVPYPETDSKYQKL
                     LRLLRLEKVKSIRLSTFPVKRLYPLDIRFKMSAGEREKEIHFIHPVTGTQHKLYLQNA
                     SSIKVPLGEALKRDLYIMQAMYEIEPALPQSDSLQFDSSFQYTEEPEVLGDIYSPSAV
                     ASIGIIGGADGPTSIFISSKESGKTVPCGMHGLPLHSCYSVPSFQKENASLFHLEGIN
                     TILYDSEEFNLQ"
     gene            complement(6147..7040)
                     /locus_tag="PK768_03635"
     CDS             complement(6147..7040)
                     /locus_tag="PK768_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQA60092.1"
                     /translation="MDTNKRGLILVIIGTLCCSTSPVITRFVVDLPSGQIAFFRLLFS
                     AFFIFVLAKVSKSRLVIDSKDIIKFLYYGLVTALHFLLYIASLKYTTIAHALCIVYTS
                     PVIIAILSALWFKEPLPGHKYVGIFVVILGIVILASFEPLLTREMIIGDILAFGSAIC
                     LSLYSIAGRKERNRYELLQYVFWVYLLASIFLLPVALSNFTVPQTFKHWIFLLLLALI
                     PNTLGHTLYNAGLRYTHAAYANLILTQEITLGTLLGYIFLREPVGKNAIIGIAVMIFG
                     IYQVLFDKKPGDKLKKMQEEC"
     gene            complement(7203..>7384)
                     /locus_tag="PK768_03640"
     CDS             complement(7203..>7384)
                     /locus_tag="PK768_03640"
                     /inference="COORDINATES: protein motif:HMM:NF027437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4829 domain-containing protein"
                     /protein_id="HQA60093.1"
                     /translation="LREITALSDEPGVLEYQVEVYFDFKKAITSEDGVWPRFVMLKKE
                     TEKSGWRIDGIGTGP"
BASE COUNT         2136 a         1522 c         1463 g         2263 t
ORIGIN      
        1 atttaatgat accataaaaa tattagcgag gtgcataaaa tgaagacaat actttgctac
       61 ggcgattcta acacctgggg ctacaatcct gacggctcgg ggcgatatcc gaaacacatg
      121 cgctggacca ctgtgctcca aaaggaactt ggggaaagtt tcgatgtaat ttccgagggt
      181 ctgaatggca ggactaccgt ttgggacgac ccggtcagag gcgattatag aaacgggaaa
      241 aagtacttgt tgccttgcct gcacactcat aagcccatcg atttagtaat attatttttg
      301 ggcagcaatg acttaaagcc caggtacaat gtgacttcaa tggaaattgc tcagagtgta
      361 gaaatgctgg tagacattgt taaaaagagc gagacgggac ctaacatgac ttcaccggaa
      421 atactcgtca ttattccccc gccgattctg atccccgaag aagtaaagca aatggattac
      481 ctgcttccgg aatatgaaaa ggcggtagac aaaagcaggc aatttcccca agctttcacg
      541 agtgtcctaa acggaaaatg ccatctgttt gactcgtcaa agtacataaa aactagtgaa
      601 atagacggaa tgcatttgga tccggaaagc cacgccatct tgggaagaga actggcaaaa
      661 tatataaagg attttcttgc gactccatag aagtttaaat acatactctt gttgtggcaa
      721 ttatttcgaa aggattgatc acaggatgta ttttaaggaa ataaaaacta cagcttcttc
      781 tacaattaag ctctttttgg gagattgttt tgaaggaatg gaaagatatc ttgagaaaaa
      841 ttcggttgat gttatagtca catcaccccc atataatatc ggcgttaatt acaataaata
      901 tgatgataaa atacctaggg aagaatactt aaattggctt ggcaactggg ctaatattac
      961 aagggaggtt cttaaagacg acggatctct ttttcttaat atcggttcta agccttcaga
     1021 tccttgggtt cccttcgaag tggcccttga aatgcgaaaa tactttgagt tacaaaatgt
     1081 aattcactgg ataaaatcaa tttatataga gcatgagagt tacggggaaa aggtagaact
     1141 aaatgtgggt cactataaac ccatcaacag taaaagattc ataaatgata cccatgaata
     1201 cgtctttcat cttacgaaac atggcaatgt cgagcttgat cgcttatcta taggagtccc
     1261 gtataaggac gaatcgaata taactcggtg ggatagtggg agaaaaggct taagatgtag
     1321 agggaacaca tggtacgtgc catataagac aattcaaagc cgcatgaaag ataggcctca
     1381 tcccgcaagt taccctgctg aacttgccaa gatgtgtata caattacatg gtgtagaaaa
     1441 aacgaagctt gtattggatc cctttatggg tataggaaat actgccatcg catgcaagga
     1501 actggatata aattgcgtgg gattcgaaat agataaggtc tacttcaatg aagctattag
     1561 aattttagag gagggagggc aattgtgcct gatttaaacc tgcttgctaa agaagagaag
     1621 ctagaaataa ttgatggtat aaaaaaagca ttcagagacg tggagtttat tggaaggttt
     1681 ttagatactt ttatgttaca aattaatctg aaggcaaatc cgccttcaaa ggtcataagt
     1741 tccatatttg aaaataactt gccggatttc ttcagtcaaa ttagttgtgc cgcggatctt
     1801 tatacaacac ttaaagacca gcatttccaa agtgcatttg atcttgcctg ggaatccaaa
     1861 atgaggaaaa ataaattaag aggacacacc atatcaaata tcgtttctgt ttgcgtagaa
     1921 tcatatttga aaaatggtag atactttact gatggtgatg gttctaatga tttgaaacgc
     1981 gtcatagatg gttcacgatg ggaaataaaa ggaagcaggt caaaaaaatt tagcctgaca
     2041 attaaccaat cccacgtggg cttagattct acttatttta tagtttacaa tggtttccct
     2101 gagcataaca agattcatgg catttatatt ttaaaggggg atgagcatat ctttacccct
     2161 aggcaagagg ggctcaacat gcgaactttt attaatgaat attacgacac ccacgtagag
     2221 tgtatatatc cgtaagaata ctccttcaaa gcttcatcct cctcagcttt catccaattc
     2281 tttgctagat tttctcaaaa gagacttatt tgatccgggt tctcgccgcc ggaatctttc
     2341 ggcgaatcgc ttgcctcctc tttagtctct ttctctataa ctaaagcacc atcgtaagta
     2401 tcatttgcaa ggatgctcat aatatgcttt tgcggagtac tgttatttag ccacaaatcc
     2461 agttgctctt ttcttacaat tagaggcatg cggtgatgta cgggctttaa ttgttcattt
     2521 gcttcggtgg ttatgattac aaaggtcact tgttctattg aattttcatc ccgtgatatt
     2581 ttgtatattc cccctagtga tattaagggt tcatcccgca ggccgatcct gtattttgct
     2641 tttttcccac tttcctcctg cttccactca taaaaaccgt tagcgggaat gacgcatctc
     2701 ctgtaaaaaa aagagctttt gaacatgggc ttgctcgtta cagtttcggc ccttgcattg
     2761 ataaccggcc tgctcttccc gctaaatgga aaaccccatt ttgcaaactg cagcatcctt
     2821 tttccgtcat cgattacaat aggggcgttt ctattcggaa aataatcacc gggcacatat
     2881 tcccataagg cttccacgtc aatggaatag tacttaatta aatcgctata taaagccatg
     2941 agcagaaaac ttccacacat cacgattcct ccctgggctg ttttctattt tcatttagat
     3001 actaaaaggt ccagtattaa tcggttgatt cttcaagttt atttagctga taatatacta
     3061 tttcaccact atcttcactg acaaagagaa gatttttttg ggtataactt gactcgttat
     3121 ttttaaattc caccgctttg ccgctttcct tttcaaaatc cctttctcct ctccggtttc
     3181 cgtgttccag gagattatgt tgcgtgcata aataccttct tcattttcaa tgtcgaagta
     3241 ctcctcgagc ccttgttcat attcttcttt ggtataagaa gaatctaaag tctcaccgtt
     3301 ttcttcagac gaactagcct gctctaaaga tagattgttt gaacagccga ttaagatgat
     3361 acagaaaata atgatggcta cacagaatct gtttttcatt tttataaaca acccctgaat
     3421 tcttggcttt attcccacaa atcgtcggcc agctttctta tattatcctt ccgtctttcc
     3481 gtctcgcttt tgtttacggt aatggtgatt atatcccctt cttttgcttc ttcgggaagg
     3541 gcttttttgg gtatgttcac aaaagtccta tcgggcagtt cgacaacagc ataatcgcct
     3601 tcaaagcggt ctatgataag gttcataaaa ttcctcctta ttggggcggc ttacagcttt
     3661 tgcagggctc atagcccctt gctttggcct cggaaagttt tatgggcacc ctgctatctt
     3721 tcagcaaatt gcagtcaccc atatgatact tcttgccggt ctttgtaata taaacggtaa
     3781 catctttagc agtccggcct gttttttcag tttttattac gatatggtct ccgtctgtgg
     3841 caaaaagcac ggttcccgat tcatcggtgc ggtatatttc gataccggcc tgttcgagtc
     3901 tagttaaaac ttcttcatgg ggatggccgt agtcatttcc tttgccgaca ctgattaccc
     3961 cataagaggg cttgactgca gccagaaatg cttcggtact ggatgtgcta gaaccatggt
     4021 ggcctagctt taaaacatcg gctttcaggt catagccact ggccagcatt tcattttctg
     4081 aaatatcctc tgcatctccc gtaaagagaa aagatgtatt gccgtatgta agttttatta
     4141 ctgccgaata gttgttaagg tcatcgtatt ccgggctgtt tggggccagg atttctactc
     4201 ttgtgttagg tgaaaagtcg ataatctctc cagcttttgc agctgctatc tttaagcctt
     4261 tattcttaat tgcgattaaa acatcttcaa aggtctttgt ggtgtgggtc accctgggca
     4321 tgtatacttt tcctatatca tatgtatcta tgaccgtgtc taaactacct atatggtctt
     4381 cgtgaggatg tgtcccgact aaaacatcga tccttcttac gccctgggca ttgagatatg
     4441 aaattatggt atctgcatca tcgttgttcc ctgcatcgat gagcatgttt tcccctgtag
     4501 gggcttggat taagatacag tcagcttgtc ccacatcaag aaaatgaact tttaaaccgg
     4561 tctcagcttc ggttgtttga acaggttctt taagagctgt actgccacag gcagcaagaa
     4621 gtactgttaa tattattagt aaacaagcaa gtagttgttt tgcattcaaa gtatgtcaac
     4681 ctttcatgta tttttatccg ggtatttttt ctacttggat tttatttgat caatttccct
     4741 agtgttattc ctcgtttaat attctaggga tgattggctt taaaatcata aaaaataaaa
     4801 atcctaataa agaaccggaa gtattagtaa tcaagtcagt gacatcaaag cttcttgatg
     4861 ataagcatcc ccaaaaggca ctaaacaatt gcgcactttc aatagcaaaa ctaaaaagaa
     4921 aacccttggc aactgtttta aaatgcttga tttctttgca tttaaagctg ttctcgtata
     4981 tatcaaaaaa ctgtttaaaa ctattgttac actctaacac actattgtaa gttaaattct
     5041 tcgctgtcat aaagtatagt atttatacct tcaaggtgaa agagagaggc attttccttt
     5101 tgaaaacttg gtacagaata gcagctatgt aacggcagtc cgtgcatacc gcagggcaca
     5161 gttttgccac tttcctttga tgagatgaaa atcgaggttg gcccgtctgc tccgccgatg
     5221 atgccgattg aggccacagc actggggctg tatatatcgc ccaatacctc aggttcttca
     5281 gtatactgaa aactgctgtc aaactgtaga ctatcgcttt gaggcaatgc aggctctatt
     5341 tcgtacattg cctgcatgat gtatagatcc cgttttaaag cttctccaag gggcaccttt
     5401 attgaactgg cattttgcaa atagagtttg tgttgtgtcc ccgttacggg atgaatgaaa
     5461 tgaatctcct tttccctttc acctgccgac attttaaaac ggatatccag agggtaaagc
     5521 cgcttgacag gaaaggtgga cagccttata cttttcactt tctccaaacg caaaaggcgc
     5581 agcagctttt gatattttga gtccgtttcc ggatacggca cagagtatcg ctggcaagca
     5641 aaaggagtgc tatcttttag aataaacgaa taggctcttc gcaccggctt tagcgcttct
     5701 tcctgctcaa gccagggtat gcatatcctg tagctagatg agtatccgcc tagtacctgc
     5761 tttccattta tccaaattct gcttatcgcc agatcctggc aagggtgttc ctgctctatt
     5821 agacgctttt gtaaaggcga aagttccttc ccttcggcta tttcatttct ttcaagaaac
     5881 tttttgagct tttcttcatc caaaattgta ataacgtcaa aggcgatgcc cttggagaag
     5941 cgatatatgg cggggatata gcgctttgcc ccagcgtaat caaattgcca atttaccctc
     6001 tgtggccatc cccaaaggcc tctgcccttg ctccacaaac tactgttgta atatactttc
     6061 attaataacc tccaataata taaaataaat acatgccgca catgttagga gcattttata
     6121 atgggggtcg cagcaataca attttattaa cattcttctt gcatcttttt caacttgtct
     6181 ccgggctttt tatcaaacaa aacctggtat atcccaaaaa tcatcactgc tatgccgatt
     6241 atggcgtttt tccctacagg ctctcttaag aatatatatc caaggagtgt ccccagggtg
     6301 atttcctgag ttaatattaa attggcataa gcagcatggg tgtaacgcaa gccggcatta
     6361 taaagagtat ggcctaaggt gtttggtatg agagctagta gcaggagaaa aatccagtgt
     6421 ttaaaagttt gtggcactgt aaagttactt aaggcaacag gtagcagaaa aatcgatgct
     6481 aaaaggtata cccaaaacac atattgtaag agctcataac gatttcgctc tttccggccg
     6541 gctatagagt atagggaaag gcatatagcg gagccgaaag ccaaaatgtc accaataatc
     6601 atttctctag taagcaacgg ttcaaaactc gctaggataa cgattcccag aattaccaca
     6661 aaaataccaa catatttatg gcctggcagc ggttctttaa accacaaggc cgatagaatt
     6721 gcgataatga caggtgaggt ataaacaatg cacagtgcgt gggcaatggt agtatactta
     6781 agcgaggcta tgtaaagcaa aaagtggagg gcagtaacaa ggccatagta caagaactta
     6841 ataatgtcct ttgagtctat cacaagccga ctcttgctga cttttgccag gacaaaaata
     6901 aaaaacgctg aaaaaagcag cctaaaaaat gctatctgcc cagatgggag gtctacaaca
     6961 aatcttgtta ttacgggact tgtgctgcaa catagggtgc ctataatgac taaaatcaga
     7021 ccgcgtttat tagtgtccat ggttcacact catttcttaa aacaatgctc acaaaaataa
     7081 ttttatcata tttggaacta atcatgtttt atagctcttc ttttgttttt ctcattccca
     7141 gtaaataaaa acagggtgct gtctctaacg ctcatgaatt ttttgtacct gtcccaaaat
     7201 attcatggcc cggttccgat gccgtcaatt ctccagccgc tcttttctgt ttctttcttt
     7261 aacatgacaa atcgcggcca gactccatct tctgaggtta ttgccttttt aaaatcaaaa
     7321 tagacttcaa cctggtactc cagcaccccc ggttcatcgc ttaaggcagt tatctccctt
     7381 agct
//
LOCUS       DAONUO010000066         7357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7621_length_7357_cov_3.885100, whole genome shotgun sequence.
ACCESSION   DAONUO010000066 DAONUO010000000
VERSION     DAONUO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_7357_cov_3.885100"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PK768_03645"
     CDS             <1..816
                     /locus_tag="PK768_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQA60094.1"
                     /translation="AHLDEITDLLRKGKDLGKLAVLTLMEPVIYFVCETIGVSMTTSS
                     EAAIVVSLVPVITTIFAAIYLKEKPTGLQAGFIFLSVSGVILMTVMKGSIQVAGNILG
                     TFILMGSAISAGIYNVLSRKLSPEFFPVEITYSMTIVGAIVFNGISVAQHLIRGDLRS
                     YFYPLINVKVLTSIVFLGAFASIIAFFMLNYLLSEMEASRVSVISNVATVISVIAGSV
                     FRNEPIYWFNIVGGIMILAGVWGTNHYGRKDDLAEAHKTNYTSKTSRKSEAEA"
     gene            975..1199
                     /gene="sspI"
                     /locus_tag="PK768_03650"
     CDS             975..1199
                     /gene="sspI"
                     /locus_tag="PK768_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein SspI"
                     /protein_id="HQA60095.1"
                     /translation="MAAAFDIRNIVLQNLAGSTREEVEGYIQETIDTREEEALPGMGI
                     LFEVVWSKSDTSERENMMNKIMQGIPATKM"
     gene            1301..2734
                     /locus_tag="PK768_03655"
     CDS             1301..2734
                     /locus_tag="PK768_03655"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HQA60096.1"
                     /translation="MKERLRELSKNRYVFSSDESQVEIHVYLSRSLFEEFTEDESIYQ
                     LYNASLLPGVVSPVIGMPDIHTGYGLPIGGVMATDYEKGVVSAGAVGMDINCGVRLLT
                     TRMLAKELNKDLLVRILQAVSKRVPLGVGKSSQLKELRKPKIEAIAHDGTEYYVKKGF
                     GRREDLDRIEDSGCIPGASAAAIPKSAMERLDQLGTIGGGNHFIEIGKISKVFDEDLA
                     SKFGLYKNHIYIMIHTGSRGFGHQICTDYSRIMWNNSDKNKTRAPQKGLACAPIFSED
                     GQNYLQAMACAANYAFANRQIITHFVREAFVEVLKESESKLGLDLLYDVAHNIAKKEK
                     YGGRWLLVHRKGATRALPANHEDNPSCYKDTGHPAIIPGSMGTGSYVVIGTDRIRETF
                     CSVNHGAGRVMSRKRAKTEFTKADLIEQMGHVVTIARSMKSLLDEAPLAYKDIDEVIF
                     TLVEAGLTKPVVKLSPMGVLKGEGSEG"
     gene            complement(2738..2974)
                     /locus_tag="PK768_03660"
     CDS             complement(2738..2974)
                     /locus_tag="PK768_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HQA60097.1"
                     /translation="MLSRQEFGSRILYRGLQGEDVRELQSKLESLGYDVGPIDGIFGP
                     LTERAVRKFQKDNDLVVDGIVGQETYGALERLVP"
     gene            3163..3417
                     /locus_tag="PK768_03665"
     CDS             3163..3417
                     /locus_tag="PK768_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60098.1"
                     /translation="MLDYGKFVAKRAKDIEISTIRYFFSMVKEVEGAISLCIGEPDFV
                     TPSHINQAAVQPLDDGRTFYTANAGHFRLRQEISNYLNRR"
     gene            3421..4329
                     /locus_tag="PK768_03670"
     CDS             3421..4329
                     /locus_tag="PK768_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQA60099.1"
                     /translation="MEYKPESEIIVTVGASQAIDVVIRTLIEPGDEVLIPQPSFVAYG
                     PCTILAGGTPVYVPTYLEDNFVLKADILEKYISPRSKLLILPYPNNPTGAVMTENELK
                     EIASVVKKYDLLVVADEIYSELTYGIEHTAFAGIEGMWERTVTINGFSKSYAMTGWRL
                     GYVAAPEGLAQEILKVHQYNVACAATISQYAGIEAMKNGDEDIAAMRARYDERRKYLL
                     TSLREMGLNCFEPRGAFYAFPSIKETGLSSEEFAKRILWEGKVAVVPGSAFGETGEGF
                     VRLSYATSMENLEEAVRRMKGFMKTL"
     regulatory      4681..4810
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4882..6435
                     /locus_tag="PK768_03675"
     CDS             4882..6435
                     /locus_tag="PK768_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA60100.1"
                     /translation="MKRQALFNVGVLLLVLLFILSACGSQKTTVDNDGGTIVIALSRE
                     IPTLDPANYRMRECENVIRNIFDGLVTKTHDGKIVPEIAESWEAISDTEWEFKIRKGV
                     TFHNGDPLTADDVVFSFERIIIEGGMEGQTSPRKGLMGPTSGVYKVDDYTVRFTMDEP
                     NPTFLHGVVHQQIIPKAYFEEVGPEGFLKQPIGAGPFKFVSGTLNDQIVLERYDDYYG
                     GSPDIPPVGPPALKRVIFKVIPEPSSAIAALKSGEVNIISGVPADMVPNLEADPNVIV
                     KTVQGTRVYMLEMNNKMPPFDNPKVRQAMNYAIDMDGILEAIYGGNATRLPGALMPYS
                     FGANPNLKPYEYNPEKAKQLLKEAGVTKLQVVIDTEPKRQNVALAMAAMLQEIGIDAQ
                     VRNWEWGVLESEIKKGTRQMYMTEWGDSWLDPVGFLNPKFRTNDRANWSHYSNERVDE
                     LLTRAERIIDSKIREEIYHEVEEILYEEAPIVFGYTLMEVEASTANVENWQPSMDGRQ
                     NMHRVRITN"
     gene            6524..>7357
                     /locus_tag="PK768_03680"
     CDS             6524..>7357
                     /locus_tag="PK768_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60101.1"
                     /translation="MKGIKILERLLATIPVILGIAVIVFLFMRLTPGDPVDIMMGREG
                     MVTEAEIEALREEFNLNEPLHVQLFIFFKGLFSGDLGNSITRGVPVIQLIKDTLPATI
                     ELAIGALLFALVIALPIGIISAVKQDSIFDRIAMAGSFLGISMPGFWLGIVLIIVFSV
                     NLGWLPSQGRLSYLVYLERITGFHVIDAIVTGNIPALLNALKHLLLPSITLGASMAAI
                     IARVMRSSMLEVLKQDYVMLARSKGLSKWLVVLRHCLKNAMMPTITVVGMQVGVLLGG
                     NM"
BASE COUNT         2238 a         1400 c         1731 g         1988 t
ORIGIN      
        1 gcccacctcg acgaaatcac cgacctgctg cgcaaaggca aggacctggg caagttagct
       61 gtcctgacat taatggaacc cgtgatatac tttgtatgtg aaaccatcgg agtcagcatg
      121 actacgtcgt ctgaggccgc catcgtagtt tcgctggttc cggttattac tactatattt
      181 gcagctatat acctgaagga aaaacctacg ggtctgcaag caggttttat atttctgtcg
      241 gtatcagggg ttattctcat gacggttatg aaaggcagta ttcaagtagc cggaaatata
      301 ctagggacct tcatactgat gggctctgcc atatctgcag ggatatataa tgtgttatcg
      361 aggaaactat cacctgaatt ttttcctgtt gaaatcactt attccatgac catagtgggg
      421 gcgatagttt ttaacggtat atcggtggcg cagcacctaa taagaggaga tttaaggagt
      481 tatttttatc ccctgataaa tgtcaaagtc ttgacttcca tagtttttct tggggccttt
      541 gcttccatca ttgcgttttt tatgctcaat tatttactat ctgaaatgga agcttccaga
      601 gtttcggtga tttctaacgt ggcaaccgtg atatccgtaa ttgccggttc ggtctttaga
      661 aatgaaccca tatattggtt taatatagtt ggcggtatca tgatccttgc cggtgtttgg
      721 gggacaaatc actacggccg aaaggatgat ttggccgaag cccataaaac aaactacaca
      781 tcaaaaacat caagaaaatc ggaggcagaa gcttgaagct gctgatatcc tccttggcaa
      841 ttccggtgca aaaaccactt ttaaccttgc cggaaataat tttgcaacat ataaaaaaat
      901 aattggcatg aaaaatgagc aaactaaaaa atgactaaac aatataatac taacagaaag
      961 gcagggagat ttttgtggca gcggcatttg atattcgaaa catagttttg caaaatcttg
     1021 caggaagcac ccgggaagaa gtggagggtt atattcagga gaccattgat acccgggaag
     1081 aagaagcact gccgggcatg ggaattttat ttgaagttgt ctggagcaag tcagatacaa
     1141 gtgaaagaga aaatatgatg aataaaataa tgcagggcat tcctgctact aagatgtaaa
     1201 ctagaaaatc ggggatttcc ccgatttttt tatacagttg aaaaacggtc cagttttaga
     1261 taaggtatag ttgagaatga aaaatttttg gaggttgaga atgaaggaaa gattaagaga
     1321 gctatctaag aaccgctatg ttttttcctc tgacgaaagt caagtggaga ttcacgtgta
     1381 tttaagccga agtctatttg aggaattcac cgaagatgag tcaatatatc agctttacaa
     1441 tgcttcttta ctgccgggtg ttgtgagccc cgtcatcggc atgcccgata ttcacaccgg
     1501 atacggccta cctattggcg gtgttatggc tacggattac gagaagggcg ttgtatcggc
     1561 aggggcggtg ggcatggaca taaattgcgg cgtcaggcta ttgactaccc gcatgcttgc
     1621 taaagagttg aacaaggacc ttcttgtcag gatcctgcag gcagtttcta aaagggttcc
     1681 cctgggtgtg ggcaaatcca gccagctaaa ggaactgagg aagccgaaaa ttgaagctat
     1741 agctcatgat ggaaccgagt actacgtgaa gaaaggtttc ggcaggagag aagatttaga
     1801 taggatagaa gacagcggat gcattcctgg ggccagtgct gccgctattc caaaatccgc
     1861 aatggagcgg ttagatcagc ttgggaccat aggcgggggt aaccacttta tcgaaatagg
     1921 taagataagc aaagtctttg atgaagacct agcttcaaaa ttcggcttgt acaaaaacca
     1981 catttacata atgattcaca cgggtagtcg gggcttcggc catcaaatct gcaccgacta
     2041 ctcaagaatc atgtggaaca actccgacaa aaacaaaacc cgtgcccctc aaaagggcct
     2101 agcatgtgct ccgatttttt ccgaagacgg tcagaattat ctccaagcta tggcctgtgc
     2161 tgccaactat gcctttgcca accggcaaat aataacccat tttgtccggg aagctttcgt
     2221 ggaagtcctc aaagaatccg agtccaagtt gggccttgat ttgttatatg acgtggccca
     2281 caacatagcg aaaaaggaaa agtacggcgg tagatggctt ttagtccaca gaaagggagc
     2341 caccagggct ttgcccgcaa atcatgagga taatccttcc tgctacaagg atacaggcca
     2401 tccggctatc attcccggga gcatgggtac tggctcctac gtcgttatag gaaccgaccg
     2461 gattagggaa actttttgtt cagtaaatca cggcgccggc cgagtaatgt ctagaaagcg
     2521 ggccaagacc gagtttacca aagcggattt aatagagcaa atgggacatg tagtgaccat
     2581 tgccagaagc atgaaatccc tgttggatga agccccactt gcatacaaag atatagatga
     2641 agtcatcttc accctggtag aagctggcct taccaagccc gtggtaaaac tctcacccat
     2701 gggagtcctt aaaggtgagg gaagcgaagg ctgacagtta cgggaccaat ctttccaatg
     2761 caccgtaagt ttcctgtccg acgatgccgt ccactaccag gtcattgtcc ttttgaaact
     2821 tgcgaacagc tctttcagtt agggggccga agataccgtc aattggcccc acatcataac
     2881 ccaggctttc aagttttgac tggagttccc tcacgtcctc accctgcaaa ccccgataga
     2941 gtatcctgga accaaattct tgacgcgata gcactattat ccctcccgat aaatcttcac
     3001 ttatatcata ttttgatgtc gatgaaagtg ttattaataa atgtacctcg tttgtaacga
     3061 tgtaccgacc tttttatata tttgcgattt tgccttgcag atgatatctt ttgaagatat
     3121 aatcttaata ataaaaattt taacttttac aggaggagat ttttgttgga ctatggaaaa
     3181 ttcgtagcca aaagagcgaa agacatcgag atttcaacaa tacgctattt tttcagtatg
     3241 gtcaaggaag tggaaggggc aatttccctg tgcattggcg aaccggattt tgtaacacca
     3301 tctcatataa atcaggcggc cgttcaaccc cttgatgatg ggcgtacctt ttatacagca
     3361 aatgccggcc actttaggct tcggcaggaa atatccaatt acttaaatcg ccgctaacaa
     3421 ttggaataca aacccgaatc ggaaattatc gtcacagtag gtgcaagcca ggccatcgat
     3481 gtagtcataa ggaccctgat agaaccggga gacgaggttt taattccgca gccttccttt
     3541 gttgcctacg ggccatgcac cattttggca ggaggtaccc ctgtctacgt gcctacatat
     3601 ttggaagaca atttcgtctt aaaagccgat attcttgaaa aatatatcag tccccggtca
     3661 aagttattaa tcctgcccta tcccaacaat cccaccggag ctgtgatgac ggaaaatgaa
     3721 ctgaaagaaa tagcgagtgt agtaaaaaaa tacgacttat tggtagtagc agatgagata
     3781 tactcggagc tgacatacgg catcgagcac accgcctttg ccgggattga agggatgtgg
     3841 gagcgtaccg ttaccataaa tggattttct aaatcttatg ccatgaccgg ctggagacta
     3901 ggctatgtgg ctgcaccgga aggattggcc caggagatac tcaaggtgca tcagtacaat
     3961 gtggcatgtg ctgcaactat cagccagtat gcagggatag aagccatgaa aaacggcgat
     4021 gaagacattg cggctatgcg tgctcggtat gacgaaaggc ggaaatactt attaacgagc
     4081 ttaagggaaa tgggccttaa ttgtttcgaa ccaaggggcg ctttttatgc atttccatca
     4141 attaaggaaa ccggcctaag ctcggaggaa tttgcaaaaa ggattttatg ggagggtaag
     4201 gttgccgttg ttcccggcag cgcctttgga gaaaccggtg aaggttttgt gagattatca
     4261 tatgccactt caatggaaaa cctggaagaa gctgtgagaa ggatgaaagg ttttatgaaa
     4321 actttatagg gcagaaagta cgaaagttgt gaaatataag caaaagagcc cagaaaatcc
     4381 gcacacttta tataaaatgc ttgaaatgtt tgtgagacta ttcctgattt atatccttta
     4441 taataatacc agagttgccc tattacaatt gccagcagta ttacaggaat atatgttttg
     4501 aaggcaattg gcaaagagga ttacaccaca aatttcataa gctaagtttt ggcaatatta
     4561 atggatataa agaaactaaa aaaagtcttt ttcaaatgaa aaaatccgac ttgaggcagt
     4621 tcagaaaaaa tagacaaagt tagaaaagtc tgctataatg gaaaaaaata aaaaaataat
     4681 agtttctacc agcttaatcc aatttttgga gcggaggacc cgatttttgg ggcgaatcct
     4741 taattttaag gagggatact ctttcatcct agcccgtcag ctaacttcgc aagcttgaaa
     4801 gaggggtaag acggtaaata aagttaaaga tattcctctt taactttatt ttttataagc
     4861 tggggataga aaggagaagg gatgaaaaga caagccttat ttaatgttgg tgtattactg
     4921 ctggttttac tgttcatctt atcagcttgt ggctctcaaa aaactactgt ggacaatgat
     4981 ggaggaacaa tagttatagc attatcgaga gaaattccca cattggatcc tgcaaattac
     5041 agaatgaggg aatgtgaaaa tgtcatacga aatatttttg acggcttagt taccaaaact
     5101 catgatggca aaatagtgcc tgaaattgcg gaatcatggg aagctatatc tgatacagaa
     5161 tgggaattca aaattcgcaa gggtgttaca tttcataatg gagatccgct gacagcggat
     5221 gatgtagtgt tttcttttga aaggataatc atagaaggtg gtatggaagg ccaaacatcg
     5281 cctaggaagg gtttaatggg tcccacctct ggggtctaca aagtggacga ttatacagtg
     5341 agatttacaa tggatgaacc aaatcccaca ttcttgcacg gtgtagtaca tcagcaaata
     5401 atacctaaag cttattttga agaagtaggg ccagaaggct ttttgaagca acccatagga
     5461 gcagggcctt tcaaatttgt atcgggaacc cttaatgatc aaatagtctt ggaaagatat
     5521 gacgactatt atggtggctc tcctgatata ccgccggttg ggccgccggc tttaaaacgg
     5581 gtcatattca aagtcatacc agaaccttca agtgccattg cagcacttaa aagtggagag
     5641 gtaaacatca tatcaggtgt cccagcggac atggtgccta atctggaagc tgatcctaat
     5701 gtaattgtaa aaactgttca aggtacaagg gtttacatgt tggaaatgaa caataagatg
     5761 cctccctttg ataaccccaa agtaagacaa gctatgaatt atgcaattga catggatggg
     5821 attttggagg caatttacgg tggaaatgca acaagactac cgggagcact gatgccttat
     5881 tcctttggcg ccaatcccaa tttaaagcct tacgagtaca accctgaaaa agctaaacaa
     5941 cttctaaaag aggctggagt aactaagctg caggtggtaa tagatacaga gccgaagagg
     6001 caaaatgtag ccttggcaat ggctgcaatg cttcaggaga ttggcattga cgcccaggtt
     6061 agaaactggg aatggggagt gctggaatct gagattaaaa agggaacgag acagatgtat
     6121 atgacagagt ggggagattc ttggcttgac cccgtaggat tcttaaatcc gaaatttagg
     6181 acaaacgaca gggctaactg gtctcactat tcaaacgaga gagttgatga gctgttaact
     6241 cgagcagaaa gaataattga ttctaagata agggaagaaa tatatcatga ggtagaagaa
     6301 attttatatg aagaagctcc tatagttttc ggttatacac ttatggaagt tgaagcgtca
     6361 actgcaaatg tggagaattg gcagccaagt atggatggaa ggcaaaacat gcatagagtg
     6421 agaatcacta attaactatg aaagcttatg cataaggggc tgtatgcccc tttactcatt
     6481 ttatttagca agcgacgaaa gtcgagaata cagaggtgga tttatgaagg gaataaaaat
     6541 acttgaaagg ttactggcta ctatccctgt tatattaggc attgcagtta tagttttctt
     6601 gttcatgcgt ttaactcccg gagatcctgt ggatattatg atgggtagag aaggcatggt
     6661 cactgaagct gagattgaag cattgcggga agaatttaat ctcaatgaac ccctgcacgt
     6721 tcaactattt atattcttta aaggtttgtt ttccggagat ttgggaaatt ccataactcg
     6781 gggagttcca gtgattcaac tcataaaaga tactctccct gccacaatag agctcgctat
     6841 aggagcatta ctttttgccc ttgtcattgc cttgcctata ggaataatct ctgcagtcaa
     6901 acaagattca atcttcgata ggattgctat ggccggttcc tttttaggaa tatcaatgcc
     6961 tggtttttgg ctggggatcg ttttgattat tgtattttct gtaaatctcg gttggttgcc
     7021 tagccagggc cgtttaagtt atttagtata ccttgaacgt atcactggtt ttcatgttat
     7081 agatgccata gtgacaggta atattccggc attacttaat gctttaaagc acctgttgct
     7141 cccttccata acccttggag cctctatggc tgctataata gctagagtta tgagatcaag
     7201 tatgctggaa gtgttaaagc aagattatgt catgttggca aggtccaagg gattgtcaaa
     7261 atggcttgta gttttaagac attgtctcaa gaatgccatg atgccaacaa ttacggttgt
     7321 aggtatgcaa gtaggtgtgc tcctgggggg gaatatg
//
LOCUS       DAONUO010000067         7245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7791_length_7245_cov_4.873574, whole genome shotgun sequence.
ACCESSION   DAONUO010000067 DAONUO010000000
VERSION     DAONUO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7791_length_7245_cov_4.873574"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..579)
                     /locus_tag="PK768_03685"
     CDS             complement(163..579)
                     /locus_tag="PK768_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA60102.1"
                     /translation="MLFGDKIKELRTSAGMSQQELANKAGLSLRSIQNYESNQRYPKD
                     VAILNKLCKALNTTIEELMKEEDNFIQEASEKYGTRGKKDAQKLVDEIGGLFAGGELN
                     EEDKDKVFRAITEMYWRAKDNNKKYTPKKYNKESIE"
     gene            700..1278
                     /locus_tag="PK768_03690"
     CDS             700..1278
                     /locus_tag="PK768_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60103.1"
                     /translation="MRKESKVMKQCALMYKGLNIERTTVLHKTKLLLKIYRPVVWSTS
                     NRAYQICESAECYCGKNLERALEYLANFAPETEQNRFSENVRSLFETHWLISLIDSTM
                     NKIYEYPDNGKMYHEILSKQYLTVSKYSEQEMLELLNMERSTYYDKKREAIDLFAICL
                     WGYTIPSMRGIFAAIEGESEIPSFFAVWTTPT"
     gene            1769..2200
                     /locus_tag="PK768_03695"
     CDS             1769..2200
                     /locus_tag="PK768_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQA60104.1"
                     /translation="MDKEKKFFIRIGNHVEQVTEEIYREYFKMLRRERYLEERDLAHG
                     RFLYSQFDNAYEDILGEEMLVDRLTEDICDSIVTKIMIEKLRECLSLLSDDELNLIIQ
                     LFYEEKSQRQLSEESGIPVMTISDRKNRILKKLKKYMENKK"
     gene            2758..5070
                     /locus_tag="PK768_03700"
     CDS             2758..5070
                     /locus_tag="PK768_03700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQA60105.1"
                     /translation="MFEIKTTDESIMQQYLKKHGNRNEFRYLFTFDKNYIFMVKENRS
                     INTYYVICLMRNCMVAIAKSKQVYSDDIKKKLIEKFIATPAYQVTLPENPNAMIEFIF
                     KKLMAASGFTIRENQIELSKMMYEGIKQNHIAICEAEVGTGKTYAYIVACVVYALYER
                     QKRSKAGILTYLDNEYCSVPCVISTSSIDLQNAIVRTYVPILSDILLKNKVIDRPLSA
                     VLRKGKEHYFCQMRYDRLINYLRSSQKAVDRELLYKLSALKIPDYGIDLDEYKGLKNH
                     IVQKINVPKACEISCPYYKDCQYIKYMDYARSSIHDFQVCNHNYYLADTMKRAKRRHT
                     LIPEHSVVVIDEAHKLPDAAMQILGKRFSSEDITIMTNVLKSNLKGNKAYLQTAKLKL
                     DSLSVLGTKFFRSLVSRINLDAVDDETSQIAILIGYFEKMLLLDMLKLIENLREYCAV
                     DISKSRSLEIMFSESVEQIETFYRTDNIIYWLENPMSEKLVSVCCIPTDLENQLHKVL
                     WKNGIPKILTSGTLSDDRGFSYFKSNAGIDKVNKNDISEMSCRSPFDYKNNALLYVSE
                     NVPFPDKQSDKYIEALADEIIRIVDAAYGHTVILFTSYFLLSRVYELARHRIKYPVLK
                     MDKSEKNIVEAFKNSGNSVLFATGSFWEGVDCPGDILSSLIIVNLPFPTPTPILEHRK
                     KQYTELKEFIKFIIFPEMLIKLKQGMGRLIRCETDTGLIAILDFRISKKGKYRKRVLD
                     ALSDYRVTDSLDQVKAFFRRVKRKEYFYQD"
     gene            5229..5366
                     /locus_tag="PK768_03705"
     CDS             5229..5366
                     /locus_tag="PK768_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017144579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HQA60106.1"
                     /translation="MEDNTLKYAVKLAFLNQLLGKKLITDKEYTAIKNDLMKKHKIIA
                     A"
     gene            5440..>7245
                     /locus_tag="PK768_03710"
     CDS             5440..>7245
                     /locus_tag="PK768_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQA60107.1"
                     /translation="MAEVKIIQASTNRINRNTAKVIERTRVAAYCRVSTDSEEQLTSY
                     NSQVIHYKALVESNPEWELVDIYADEGISGTQTEKRIEFQRMINDAIDGRIDLIITKS
                     ISRFARNTLDTLKYVRLLKEHNVAIFFEKENINTLTMNGEMLLVILSSLAQQESESIS
                     SNVKMGLKMKMKRGELVGFHGCLGYDYNPIDKTLTINEEEAEIVRYIFRRYVEGAGAF
                     VIAKELTKLNYKTKKGSAKWHESAVRGIIKNEKYKGDILLGKTFTVDPLTHRRLENMG
                     EEEKYYISGNHEPIISEELFEEAQKILQVRSSKHNNKGRTDKYSKKYAFSSIIKCGFC
                     GSTAIRRTWHSKSVHEKYVWSCMTSVKEGKKYCPDSKAIDEKYIEQAFVESFNMLCSN
                     NRDIIEEFLNDMEETLSNLDTAKELKRVQKEIDSIENKLSKLVDMRVDEVIDKETYEA
                     KYTEFSGKLEKLKSERLELQLSVDEKNDLKKRMKAFREVYEKNEPLTEFDRHVFESVV
                     EEIILGKVDEHGNKKPYFITFIFRTGFKMEADGEMKKSKSKGSKSKSNSENLCSYSAY
                     DTCGVRDIDDKCKKQISAIASRICRNKYQTPTQYIK"
BASE COUNT         2521 a         1124 c         1464 g         2136 t
ORIGIN      
        1 aactccaata gatttacata attcaaaagg gtttctggtc ttgtaccgtg tgattaaatc
       61 ggaagcaaca gattgaatat agtctttgtt cggcatacgt gattcacccc ctttagtatc
      121 ctcattatac caaataatgt gtacgataaa acggacagca atttattcga tagactcttt
      181 gttatacttc ttaggggtat atttcttatt attgtccttg gctctccagt acatctcagt
      241 gatagctcta aaaactttgt ctttatcttc ttcattcaat tccccaccgg caaaaaggcc
      301 ccctatctca tcaactaatt tttgagcatc ttttttgcca cgagtgccgt atttttcaga
      361 tgcttcctga ataaaattat cttcttcttt catcagttct tctattgtgg tattcaatgc
      421 cttgcagagc ttattcaaaa tcgctacgtc tttggggtat ctttggttgc tctcataatt
      481 ttgaatagat cgtaaggaaa gccctgcttt atttgctaat tcttgttgac tcattcctgc
      541 cgaagttctc agttctttaa ttttatcacc aaacaacatg atattggctc cttcctaagc
      601 aatgcgtttt tatatgctcg atatgcaaca tcgttcgtta tatatgttac acttgattga
      661 aacagatgtc aatatgaaaa ttaaggaagg acagatgcta tgcgtaaaga gagcaaagta
      721 atgaaacaat gcgcactaat gtataaagga ttaaatatag agcgcactac ggttctacat
      781 aaaacaaaac tgcttcttaa aatatatcgt ccggtcgtgt ggtcaacatc aaatagagcc
      841 tatcaaatat gtgagagtgc cgaatgttat tgcggtaaaa atttggagag agcattagag
      901 tacctggcta attttgcccc tgaaactgaa caaaataggt tttcagaaaa tgtacgatcc
      961 ttatttgaaa cacattggct catttcattg attgattcca caatgaataa aatttatgaa
     1021 tatccagata acgggaagat gtatcatgaa atattaagta aacaatattt gacggtttcc
     1081 aaatactcag agcaggaaat gcttgaatta ttaaatatgg agcgtagtac atattacgat
     1141 aaaaaacggg aagcgattga tttattcgct atttgccttt ggggctatac aattccatca
     1201 atgagaggaa tttttgctgc aatcgaagga gaaagtgaga taccgagctt ttttgctgtt
     1261 tggacaaccc cgacttaatc ccgacttaat cccgacaaag tcccgattaa ttccctactg
     1321 ctttccgact tccaacatgt tacactgtct atactggtga attatggcat aaattaactt
     1381 gaatatagaa gcttacagcc tcaatatcca aataggattt tggggctttt ttaatgccct
     1441 gttttatcag taaatatggc ggcgattaag gccggaggtg attagttcac ttccggcctt
     1501 tttttatgcc cggaaacagg agaaagttgt atgaaaaggc ggggaactta cagctctcgt
     1561 tttcatatta agcatgaaca ttccttaaaa caaaacgacg tgtttttaga cgtttttgta
     1621 ttaaaggcat acttcaggaa ttttggaggt gcctttatga agacctgtat ctatagcaca
     1681 aatcggtggg aaacaagcat cgcccgttat ccatgacttt caaatttcgc ttgaacattc
     1741 atcaaaacga aatttggagg ttaagaaaat ggataaggaa aagaagtttt ttatacgcat
     1801 tggaaatcac gttgaacagg taacagaaga aatttacaga gagtatttca aaatgctcag
     1861 aagagagcgc tatttggaag aacgggattt ggcacatggc aggttcttgt attcacagtt
     1921 tgataatgcc tatgaggata ttctgggaga agaaatgctc gtggataggc ttacagagga
     1981 tatttgtgat tctattgtca ctaaaatcat gattgagaaa ttaagggagt gtctatccct
     2041 tttgtctgat gatgaactga atttaatcat ccagttattt tatgaagaaa aaagtcagcg
     2101 acagctatca gaggaaagtg gcataccagt aatgaccata agtgatcgaa agaacagaat
     2161 cctaaagaag ctaaaaaaat atatggaaaa taaaaaataa ttccgtacac ccccctcact
     2221 tttccggctt agaagtgagg gggttatttt ttactctcct tatgctcctt gaaaactgaa
     2281 catacggtat atcaggaaaa ccacctttgc agccagaaat ggcaagcaac gaaagcggag
     2341 cgccatgacc gtcctggacg cgagcgaaca cctccggctt gaaatagcca gtggaatggc
     2401 taattgcgat gaccgacaaa gggataaata atgatacttc tgccttgaac gcctcaccgc
     2461 attgggcagc ccgcaacgaa tgacgggata acgctcattt ggcttgagaa cttatagcca
     2521 tgtcagctta tatgatgttt tccaaagaca aactgatata tcccctcagt tgtatcaggg
     2581 gcgttgagaa caaatatgat acacaccaat tctactgttc aaacgaattg cttgacagca
     2641 gaaactatgc ggtggcctgt acgaaagctg attattcaaa atcgctcaaa tagtagtaca
     2701 ggccaccgta tcattgtgct atcaagtatc ccacataata cgaaggagag gaaaattatg
     2761 tttgagataa aaacgacaga cgaatcgata atgcaacaat atctcaaaaa gcatggaaac
     2821 agaaacgaat ttcgttatct ttttacattt gataaaaact acattttcat ggtgaaagaa
     2881 aatagaagca taaataccta ctatgtaatt tgcttgatga gaaattgtat ggttgctatt
     2941 gctaaaagca agcaagttta ttctgatgat ataaaaaaga aactaataga gaagtttatt
     3001 gctacacctg cttatcaagt cacacttcct gaaaacccga atgccatgat tgaatttatc
     3061 ttcaaaaaac tgatggcagc aagcggattt acaatcaggg aaaatcaaat tgagctatct
     3121 aaaatgatgt atgaaggaat taagcagaat catattgcta tttgcgaagc agaggttgga
     3181 acgggaaaaa cctatgctta catagttgct tgtgttgttt atgctcttta tgaaagacaa
     3241 aagcgtagca aagcaggtat acttacatat ttggacaatg aatactgctc tgtcccttgt
     3301 gttatatcaa catccagtat tgatttgcaa aatgccattg ttcgcactta tgtcccaatc
     3361 ttatctgata tattgctgaa gaataaggtg attgatcgtc ctttaagtgc agttttaaga
     3421 aaggggaaag aacattactt ttgtcagatg cgatacgacc ggttaataaa ttatttaaga
     3481 agtagtcaaa aagctgtgga cagagaactg ctttataaac tttctgcatt aaagataccg
     3541 gattatggaa ttgatttaga tgaatataaa ggattaaaaa accatatagt ccaaaaaatt
     3601 aatgtaccca aagcttgtga aatttcctgt ccttactata aggactgcca atatatcaag
     3661 tatatggact atgcgagatc atcaatccat gactttcagg tatgtaatca taattattac
     3721 ctggcagata caatgaaacg tgccaaaagg aggcatactc ttattccaga acactctgtg
     3781 gtagtaattg atgaggctca taaattgccg gatgcagcta tgcaaatact tggaaaaaga
     3841 ttttcaagtg aagatattac gattatgact aatgtgctaa aaagtaatct gaaaggcaat
     3901 aaagcatatt tacagacagc aaaattgaag ttagacagtt tatctgttct ggggacgaaa
     3961 ttttttagga gcttggtttc aagaattaac ctggatgccg tagatgatga gacatcacaa
     4021 atagctatat taataggata ctttgaaaaa atgctgctcc tggacatgtt aaaacttatt
     4081 gaaaatcttc gggaatactg tgcggtggat atttcaaaga gcaggtcatt ggagattatg
     4141 ttttcagaat cagtagaaca gattgaaacc ttttaccgga ctgataatat catttattgg
     4201 cttgaaaacc caatgagtga aaaattggtt tctgtctgct gtattccaac agatttagag
     4261 aatcaattgc ataaagtgct atggaaaaat ggaatcccaa agattttaac ttccggaaca
     4321 ttatcagatg ataggggttt tagctacttc aaaagcaatg ctggaataga taaggttaat
     4381 aaaaatgata tcagtgagat gagctgtcgt tcgccatttg actataaaaa taatgcatta
     4441 ctttatgtca gtgaaaacgt accatttcct gataaacaaa gcgataagta tattgaggct
     4501 ttagcggatg aaattataag gattgtggat gcagcctatg ggcatacagt gattttgttt
     4561 acttcctact tcttgttatc aagagtgtat gaattggcaa gacacagaat caaatatccg
     4621 gtcctgaaaa tggataaaag tgagaagaac attgtagaag cttttaaaaa cagcggaaac
     4681 agtgttcttt ttgctacagg ctcgttctgg gaaggtgttg actgtccagg agatatttta
     4741 tcttcactta ttattgtcaa tttaccattc cccacaccta caccgatttt ggaacatagg
     4801 aagaaacagt atacagagct gaaagagttt attaaattta ttatttttcc tgaaatgctc
     4861 attaagttga agcaaggcat ggggcgattg attcgttgtg aaacggatac cggtctcatc
     4921 gccattttag atttcaggat tagtaaaaaa ggaaagtacc gcaagagagt gcttgatgct
     4981 ctatcagact atagggtgac agattctctt gatcaggtta aagcattttt tcgtagagtc
     5041 aaaagaaaag aatattttta tcaagattga gtgagcggtt atgagaatag ctgctcactt
     5101 ttttatggaa ggagacgggt agggatgagc cttacctatc tcctttcttt atgtcagaac
     5161 aaatgcattt agggtatacg gagtcatttt tgtgcttaat ggtgtgaatt tgtaaggagg
     5221 tctttttaat ggaagataat actttaaaat atgcggtgaa attagctttt ttaaaccaac
     5281 tcttaggtaa gaaattgatt acagataaag aatatactgc tattaaaaat gatttaatga
     5341 aaaaacataa aattatcgct gcataactga tgttgaaatg tattcttatc cgtaatataa
     5401 ttagtgttga ataagaatac ataaaggggt atggataata tggctgaagt aaagataatt
     5461 caggcaagta caaacagaat taatcgtaat actgctaaag taatagaacg cacaagggtt
     5521 gccgcttatt gtagagtcag tactgattct gaggaacaat taacaagtta taactcgcag
     5581 gtaatacatt ataaagcttt agttgaatca aatcctgaat gggagcttgt tgatatttat
     5641 gctgatgagg gtataagtgg tacacagacc gaaaaaagaa tcgaatttca aagaatgatt
     5701 aatgatgcaa ttgatggcag gatagattta ataattacaa aatctatttc acggtttgcc
     5761 cgtaatacat tagatacgct caaatatgtc cggctgttaa aagaacataa tgttgcaatt
     5821 ttttttgaaa aagaaaacat caatacatta acaatgaacg gagaaatgct tttagtaatt
     5881 ttaagctcat tagcacaaca agaaagtgaa tcgatctcat ctaatgtaaa gatggggtta
     5941 aaaatgaaaa tgaaaagagg cgagttagtt ggatttcatg gatgcttggg gtatgactat
     6001 aatccaattg acaaaacgct taccattaat gaggaagaag cagaaattgt aaggtatata
     6061 tttagaagat atgttgaagg tgcaggagct tttgtaatag ccaaagagct tactaagctc
     6121 aattataaaa ctaaaaaagg tagtgcaaag tggcatgaaa gtgcagtcag aggaattata
     6181 aaaaatgaaa agtataaggg cgatatatta ttggggaaga cttttacggt agaccctttg
     6241 acacatagaa gattagagaa tatgggggaa gaagagaaat attacatcag tggaaatcac
     6301 gagccgatta tttcagaaga attgtttgaa gaagcacaga agattttgca ggttagaagt
     6361 agtaaacata ataataaagg tagaacagat aaatatagta agaagtacgc tttcagcagc
     6421 ataatcaaat gtggattttg tggcagcaca gcaataagac gaacctggca tagtaaatct
     6481 gttcacgaaa aatatgtgtg gagctgcatg acatcggtta aagaaggtaa aaagtattgt
     6541 cctgacagta aagcaattga tgagaaatat attgagcagg cttttgtaga gtcgttcaat
     6601 atgctttgca gcaataacag ggatataatt gaagagtttt tgaatgatat ggaagaaact
     6661 cttagcaatt tggatactgc gaaggagtta aaacgtgtac aaaaagaaat cgattctatt
     6721 gaaaataagc tgagcaagct tgtcgatatg agagtagatg aagtaataga caaggaaaca
     6781 tatgaagcca aatatacaga gttttccggt aagcttgaaa aacttaaaag tgaaaggtta
     6841 gaacttcagc tttcagttga tgaaaaaaat gatcttaaga aaagaatgaa agcttttagg
     6901 gaagtctatg aaaaaaatga accacttaca gaattcgacc ggcatgtttt tgaaagcgta
     6961 gttgaagaaa tcattctggg gaaggtagat gaacatggga ataaaaagcc ttatttcatt
     7021 actttcatct tcagaacggg ttttaaaatg gaagcagatg gcgaaatgaa aaaatcaaag
     7081 tcaaaaggtt ctaaatcaaa gtcaaatagt gaaaatttgt gttcttattc cgcatacgac
     7141 acatgtggag tgcgtgacat tgatgacaaa tgtaaaaaac aaataagcgc gatagcgtca
     7201 agaatttgta ggaataaata tcaaactcct actcaataca tcaag
//
LOCUS       DAONUO010000068         7200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7856_length_7200_cov_3.978167, whole genome shotgun sequence.
ACCESSION   DAONUO010000068 DAONUO010000000
VERSION     DAONUO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7856_length_7200_cov_3.978167"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1584
                     /locus_tag="PK768_03715"
     CDS             226..1584
                     /locus_tag="PK768_03715"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HQA60108.1"
                     /translation="MSQKQEMLKEREGFASRIGFIFAAAGSAIGLGNIWRFPWLVGAY
                     GGAAFLAMYLIIVALVGITMFMGEVALGRYAQRSNVGAFKKVNKSFSWLGSIGLIAGF
                     MILSFYSVVGGWVIYYCFRAVIGFNMTDPTLTEQLFVGFISNPLSPLFFHALFMALTI
                     WICYNGVKDGIEKYSNIMMPALFVIILILAVRSLMLPNAIEGIKFYLVPDISKFSAEA
                     ALAALGQVFFSLSLGMGSILTYGSYLSKKENIPNVSLIVPLMDTTIAFIAGIVIFPAV
                     FSYGFEPSSGAGLTFVTLPAVFSEMPLGQLFGAAFFFLLFLAALTSAISLLEPLIAYM
                     IEEHGWSREKAALIIGGIIFIVGCFASLSMGPLSGFKIGGLVFFDQLDWVSNNLLLPI
                     GGMLTALFISWIWGTKNVLNEVTNEGILDFGLGKFWANVMLKFIAPALVFIVFLTGLN
                     IF"
     gene            complement(1631..2830)
                     /locus_tag="PK768_03720"
     CDS             complement(1631..2830)
                     /locus_tag="PK768_03720"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQA60109.1"
                     /translation="MFLSEKARVISPSPTLAVDAKAKRMKREGYDVISFGAGEPDFDT
                     PDYIKSAALSAIDRGFTKYTPVGGTKEIKNAVSDYFKKQIGISYEHDEITVSNGAKQC
                     IFNALYCLIDDGDKVLIPSPYWVSYPEMVKLCGGIPVFVPTIEEGFMLKAETIEKIID
                     KQTKVLIINSPNNPCGCVYSKEDLQHIAELAVEKNLFVISDEIYGELIYDDAEHVSIA
                     SFDNMFERSLVVNGVSKSFAMTGWRIGFAAGPKELIKAMENLQSHTTSNPNSIAQSAS
                     AEALNVLTQKGDGVIKAMVDEFSKRRKYMVDRINSMKYLSSTVPLGAFYVFIDISETF
                     GKQCNGSTIINSTDFAQLLLENYHVAVVPGVAFGADNYVRLSYATSMENIKRGLDRID
                     QFTLSLK"
     gene            complement(2976..4073)
                     /locus_tag="PK768_03725"
     CDS             complement(2976..4073)
                     /locus_tag="PK768_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA60110.1"
                     /translation="MKKGSIIRYFPGNNTPDGYFSYFESILPWKRAQRIIIIKGGPGV
                     GKSTMIRKITKELVDRGLNIELLHCTADSNSLDGLIIKDYSIAVVDGTAPHMIDPKFP
                     GCVDEIVNLGDYWNEAELYKQKERIFLLQEKVKHLYARVYNYLKAAKIIFNNLEQAYS
                     TLANRRLIEAKTEEIIEDVFKGVKRRDKISLQRRMFASGITPEGSIHFLDELFDKVSK
                     RFILVGNPGTGKSAVLKKIEELGTSKGLDMDIFYCPMNPQKIEHLLIKDLDIGFITSV
                     RPHMILNTRENDVFIDMNSVLDCSRLKDIEEDISQDSLLAWSLFDKAVKTLIQVKQTR
                     DELEGIYSSNMNFQVAEKVGEQILAKILSYI"
     gene            complement(4144..4308)
                     /locus_tag="PK768_03730"
     CDS             complement(4144..4308)
                     /locus_tag="PK768_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmA family protein"
                     /protein_id="HQA60111.1"
                     /translation="MEEGKLELIAKKEIAYNDELYKLIDFLNKNLKNKNIILGISKNK
                     DKAIISVYET"
     gene            complement(4337..5098)
                     /gene="surE"
                     /locus_tag="PK768_03735"
     CDS             complement(4337..5098)
                     /gene="surE"
                     /locus_tag="PK768_03735"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQA60112.1"
                     /translation="MNILITNDDGINSAGLIALVQTISEIANVTVVAPDRERSATAHA
                     ITMHKPLRVEEIEIPGCDALGYKVNGTPSDCVKLALEALLNNAPDLVLSGINRGANLG
                     TDVIYSGTVSAAFEAALLGVPGIALSVTSRENVEFNHAADFAKKLCQQVSRKIFPKDT
                     LLNVNIPNIKPERIAGIAITHLGTIRYKDSFERRTDPRGKVYYWLAGEAIEDTNDKGS
                     DVWAIKNNFISITPILIDLTKYDIIDTIRDWNLKL"
     gene            complement(5110..6276)
                     /locus_tag="PK768_03740"
     CDS             complement(5110..6276)
                     /locus_tag="PK768_03740"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQA60113.1"
                     /translation="MNKKEAYGVLSKLCKVNLPFIWTSIWFWAGKYARALDYINTVYD
                     ENCAVLDVYCWRGILYYFSNDHNYAIDNLEKAERANPKKLEIKYLLAEIYFESAHIKK
                     AQIRYRALLGNSDYKTFGLYGIGNCLLKTNHHVEALGFFNKALSYAKLEYIPKILNKK
                     GLCLMELNKIEEAIRCFEECVRFVPTDYSVQLNLALALGKSKKYKEAAVVYKNIIKKM
                     PHNLIVINNFASCLAACHDYNEALNYCNKGLKIDPGNPDLLINKGYCLYKLGRYNNSL
                     ECLNEAEKILKDDIILTNNKALCLMALEKYDDALKLFDSLLKSNNSDDLMLNKAYCLV
                     KKGMYSEALIYLLKIKDERIKNFDYFTLKGICFEHLGNHEAAVENYNKSLNMAR"
     gene            6882..6958
                     /locus_tag="PK768_03745"
     tRNA            6882..6958
                     /locus_tag="PK768_03745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6916..6918,aa:Ile,seq:cat)
     gene            6964..7039
                     /locus_tag="PK768_03750"
     tRNA            6964..7039
                     /locus_tag="PK768_03750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6997..6999,aa:Thr,seq:tgt)
     gene            7049..7134
                     /locus_tag="PK768_03755"
     tRNA            7049..7134
                     /locus_tag="PK768_03755"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7084..7086,aa:Tyr,seq:gta)
BASE COUNT         2080 a         1427 c         1210 g         2483 t
ORIGIN      
        1 catggtatcg ccccctgtta gttttatgtt accatgatac aaggattttt tccaagtgcc
       61 aagatgtaaa tgtattgaaa tgcatgaaca accagagtgc tttttttatt attagcatac
      121 tatatttttt attcttacat acccacataa actgaaattt ggtataatgg tggagaaagt
      181 tttaaaatcc aacaaaagta attttacaaa aggagatgat atgtcttgag tcaaaagcag
      241 gaaatgttaa aggagcgaga aggtttcgca agtcgtatcg gttttatttt tgctgctgct
      301 ggttctgcta ttggtctagg taatatttgg agattcccgt ggctcgtagg agcttatgga
      361 ggcgctgctt ttttagcaat gtatttaata attgtcgcgt tggtaggtat cacaatgttc
      421 atgggcgaag ttgctctcgg tcgctacgca caaaggtcca atgtaggtgc ctttaaaaaa
      481 gtcaataaat cttttagttg gttgggaagc ataggcctta ttgcgggctt tatgattttg
      541 tcattttata gtgtggttgg cggatgggtt atctactatt gctttcgcgc cgttattgga
      601 tttaacatga cagatcctac cctaactgaa caactttttg ttggttttat ttctaatcct
      661 ctttcccctt tgtttttcca cgctctgttt atggccttaa caatttggat ttgttataac
      721 ggcgtaaaag acggtattga aaagtattca aatataatga tgccagcact attcgttatt
      781 atactaattt tagcagttcg ctctttaatg ctaccaaacg caatagaagg tattaagttt
      841 tatcttgtgc ctgacatctc gaagttttct gcagaagcag cattggcggc actggggcaa
      901 gtttttttct ctttgagttt aggaatgggc tcaatcttaa cgtatggcag ctatttgagc
      961 aaaaaagaaa acatacctaa tgtatcattg attgttccct tgatggatac aactatagct
     1021 tttatagccg gtatagtcat attccccgct gttttttcat atggctttga accctcttcg
     1081 ggtgcaggtt taactttcgt cactctgccg gcagtattta gtgagatgcc tctaggccag
     1141 ctttttggag cagcattttt cttcctattg ttccttgcag ctctaacttc tgctatttca
     1201 ctattagaac cccttatcgc ctatatgata gaggaacatg gatggtctcg agaaaaagca
     1261 gcacttatta ttggtggaat aatatttatt gtgggttgtt ttgcttcgtt atccatgggt
     1321 cctttaagcg gatttaaaat tggcggcttg gtgttctttg atcagcttga ctgggtatct
     1381 aacaacctcc ttttgcctat cgggggcatg ttgacagcac tatttatatc atggatatgg
     1441 ggcaccaaaa atgttttaaa tgaagtaact aacgagggaa tccttgattt tggattgggc
     1501 aaattttggg ccaatgtcat gttaaaattt attgctccag ctttggtttt tattgtgttt
     1561 ctaacaggat taaatatatt ttagtgcata caaaaaaaca gcgcctgaaa aggtgctgtt
     1621 ttttaatgcc ttattttaaa ctcagagtaa attggtcaat acggtctaaa cctcttttaa
     1681 tattctccat agatgtggca taggatagtc ttacataatt atctgcgccg aatgctactc
     1741 ccggcactac tgctacgtga tagttttcta gcagcaactg tgcgaaatcg gttgaattaa
     1801 taattgtact accattacat tgcttaccga aagtctctga tatgtctata aaaacataaa
     1861 atgcgcccaa gggtacagta cttgaaagat atttcatact atttattctg tctaccatat
     1921 actttcgccg ctttgaaaac tcatcgacca ttgcttttat aactccatcg cccttctgtg
     1981 tgagaacatt taaggcttcc gcactggcac tttgagcaat tgagttggga ttggatgtag
     2041 tatggctttg tagattttcc atagctttaa taagctcttt tggccctgcc gcaaacccaa
     2101 tcctccaccc tgtcatagca aaagacttgg aaactccatt tacaaccaat gaccgttcaa
     2161 acatattgtc gaaggaagca atgctaacgt gctctgcgtc atcataaatc aattctccgt
     2221 aaatctcatc tgaaataaca aaaagatttt tttctacagc caattcagct atatgttgta
     2281 aatcctcctt tgaatataca caaccgcaag gattgtttgg actattgata attagtacct
     2341 tggtctgttt gtcaataatt ttttctatag tttccgcttt taacataaaa ccttcttcaa
     2401 ttgtcgggac aaataccggt attcccccac agagcttaac catttctgga taacttaccc
     2461 aataaggact aggaattaaa accttatcac catcatctat taggcaatat aatgcattaa
     2521 agatgcactg ctttgcacca ttggataccg ttatttcatc gtgttcatat gatattccga
     2581 tttgtttttt aaagtaatcg gatacagcat tttttatctc tttggttcct cctaccggtg
     2641 tatatttggt aaaaccacga tctatagcag ataaagctgc tgatttaata taatcaggcg
     2701 tatcaaaatc cggctcacca gcgccaaagc ttattacatc gtatccttct cttttcatcc
     2761 gttttgcctt ggcatcaaca gctaaagttg gagatggact gatgactcga gctttttctg
     2821 aaagaaacat ttcaagacct cctactcaat aatgtttttc tttatattct ccaaaaaatt
     2881 taaatttcct gcaaagggta aaataaaaat ctttttttgg gaaaaagaaa aaggggcaga
     2941 atacacccct cccacaaaat ctaaataggc aatatttaaa tatatgatag gatcttggca
     3001 agtatctgtt ccccaacttt ctcagcaact tgaaagttca tatttgatga gtatattcct
     3061 tcaagctcat cacgggtttg cttaacctgg attaaagtct tcacagcttt atcaaataag
     3121 ctccatgcaa gcaaactatc ttgactgata tcttcctcta tatcttttag tctggaacaa
     3181 tccaaaaccg aattcatatc tataaataca tcattttctc tcgtgtttaa tatcatgtga
     3241 ggtcttactg aagtaataaa tccaatatca agatctttta tgagcaaatg ctcaattttc
     3301 tggggattca tcgggcaata gaaaatatcc atatccaacc ctttactggt ccccagttct
     3361 tcaatttttt tcaatacagc agatttgccg gtgcccggat tgccaacgag gataaagcgc
     3421 ttactcactt tatcaaatag ttcgtctaaa aaatgaatgg aaccttcggg agttataccg
     3481 cttgcaaaca tacgcctttg aagagagatt ttatcccttc gctttactcc tttaaataca
     3541 tcctctataa tttcttcagt tttagcctca attaatcggc ggttagctaa ggtgctatac
     3601 gcctgttcaa gattattaaa tatgattttg gctgccttta aatagttata tacccttgca
     3661 tatagatgtt ttactttttc ttgcaaaaga aagattcgtt ctttttgctt ataaagctca
     3721 gcttcgttcc aataatctcc aagatttact atttcatcaa cgcatccggg aaatttagga
     3781 tcgatcatat gcggcgccgt tccatcaaca acagctatac tataatcttt gattattaaa
     3841 ccgtctaaag aattgctatc ggcagtgcag tgaagcagtt ctatatttaa accgcggtcg
     3901 actaattctt tggtgatttt tctaatcatc gtagatttac caacacccgg accgccttta
     3961 attattatta ttctctgggc ccgtttccaa ggcaaaatag attcgaaata agaaaaatat
     4021 ccatcaggcg tgttattacc tggaaaatac cttattatgc tacctttttt cattatgcgt
     4081 tccctccaat aaaattgaaa tatcattata gtatacgtgg agggaactgc attggtgcaa
     4141 attttacgtc tcgtatactg aaattatggc tttatctttg tttttagaaa tacccaatat
     4201 aatatttttg ttttttaaat ttttatttaa aaaatcaata agcttgtaca gctcatcatt
     4261 ataagctatt tctttttttg ctattagttc gagtttgcct tcttccattt tttaatacct
     4321 tcctaatttc tttattttat agtttcaaat tccaatctct tatagtatct ataatatcat
     4381 attttgtcaa atctataaga attggtgtta tagagataaa attgtttttg atcgcccaaa
     4441 catcggaacc cttatcatta gtatcttcaa ttgcctcacc ggctaaccaa tagtaaactt
     4501 tacctcgtgg atcagtgcgt cgctcaaaac tgtctttata tcttatggtt cccaaatggg
     4561 ttatagcaat acctgctatt ctctcgggct taatgtttgg tatgtttaca tttagcaaag
     4621 tgtcttttgg gaaaattttt ctggaaactt gttggcacag cttttttgca aaatctgcag
     4681 catggttaaa ctcaacattt tcacgacttg tcactgacaa tgcaataccg gggacaccta
     4741 aaagagcagc ttcaaaggcc gctgaaacag ttcctgaata tataacgtct gttcctaggt
     4801 ttgctccacg gttaatcccg gacaatacga gatcgggtgc attatttagc aaagcttcca
     4861 atgcaagttt aacacaatct gaaggagttc catttacctt atagcctaaa gcatcgcaac
     4921 ctggtatttc gatttcttct actctcaggg gcttatgcat agttattgcg tgagctgttg
     4981 cactccgctc cctatctgga gcgacaacag tcacgttggc aatttccgat atagtctgaa
     5041 ccagtgcgat taacccggca gaattaattc catcatcatt tgttataagg atattcaaat
     5101 gctacactcc tatctagcca tatttaaaga tttgttgtaa ttctccacag ctgcttcatg
     5161 attccctaga tgttcaaagc agattccttt cagcgtgaaa taatcaaagt tctttatccg
     5221 ttcatctttg attttcaaaa gatagataag cgcttcgcta tacatacctt tttttactaa
     5281 gcaatatgcc ttgtttaaca tcaaatcatc cgaattatta ctctttaata aactgtcaaa
     5341 aagctttaag gcatcatcat atttttcaag ggccattaag cacaaagctt tattattagt
     5401 taatattata tcatctttta atattttttc agcttcattt agacactcta gggaattatt
     5461 atatcgacct agcttgtaca gacaataacc tttatttatt agcaaatcgg gattgcccgg
     5521 atctattttt aagcctttat tacagtaatt taaagcctca ttgtaatcat gacaggccgc
     5581 caaacatgat gcaaagttat ttataactat gaggttatgg ggcatttttt taattatatt
     5641 tttataaaca acggctgctt ctttatattt ttttgatttc cccaaagcta atgccagatt
     5701 taattgtacc gaataatccg ttggcacaaa ccttacacat tcttcaaagc atcttattgc
     5761 ttcttcaatt ttatttagtt ccattaaaca taagcccttt ttatttagta ttttagggat
     5821 gtattcaagc ttggcataag acaaagcctt attaaaaaat cccaaggcct caacatgatg
     5881 gttcgttttt aacaaacagt tgccaattcc atataagcca aaagtcttat aatcactatt
     5941 ccctaataaa gccctatacc gtatctgggc tttttttata tgagcagatt caaagtaaat
     6001 ctctgccaat aaatacttta tttcaagctt ttttggattg gctctttcag ccttttccaa
     6061 attatcaatg gcataattgt ggtcatttga gaaataatat agtattcctc tccaacaata
     6121 aacatctaga acagcacagt tttcatcata tactgtattt atgtaatcca aagccctcgc
     6181 atattttcct gcccagaacc aaatacttgt ccaaataaag ggcaaattta ctttacatag
     6241 tttacttaaa actccgtaag cttctttctt attcatattt atatcccccc atttatttct
     6301 aacatattat tctattttat cctgtttttt ccttcccatt ttggaaaatt aagttgctta
     6361 aaattcatga aaggacggaa cttaacctta ccatcttacc ctttatgttt attttccaac
     6421 ttgttttgtt caaaaagcgc acaatttgaa tggcaaaact ctgccggtaa atccataacc
     6481 aaatttaaca atttacagga aaaccatgtt tgacttgaac cgcgaattga aaagatctta
     6541 tgcggcacta agtatttgca aggtttattg gccataatcg atggaacaat acatccttta
     6601 caagcccagt tctttttgat atcatttaca gtcaaaagag cgtctgcaag gacacatcta
     6661 tatataaacc caacggaatc cagtaattta tagcttttaa atacttttaa ataccggcag
     6721 cttttataca tgctgatagc ctccttcata aaagcctttg ttcataaatt atgaggggca
     6781 tatgtataag gtgcaaaata cactttatga cttcaataaa aaaatataat taaatgggct
     6841 tgacgaaaaa aaaaatctat tgtatactat taattgcgtt cgggcccata gctcagtcgg
     6901 ttagagcagc cggctcataa ccggccggtc ctaggttcga gtcctagtgg gcccaccata
     6961 cttgccgacg tagctcagct ggtagagcag ctgatttgta atcagcaggt cgcgggttcg
     7021 agtcccatcg tcggctccat aattacaagg agaggttccc gagttggcca aagggggcag
     7081 actgtaaatc tgctggcaca gccttcggtg gttcgaatcc acccctctcc accatctgaa
     7141 tacgggccat tagctcagtt ggtagagcac ctgactctta atcagggtgt cctgggttcg
//
LOCUS       DAONUO010000069         7138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7946_length_7138_cov_3.353523, whole genome shotgun sequence.
ACCESSION   DAONUO010000069 DAONUO010000000
VERSION     DAONUO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7946_length_7138_cov_3.353523"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2433)
                     /locus_tag="PK768_03760"
     CDS             complement(<1..2433)
                     /locus_tag="PK768_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQA60114.1"
                     /translation="MKVKILVVDDSATDRFIIKNMLSDYIIFTASDGVEAIRMLEEHD
                     GINLLILDLKMPKMDGFQVLEYLKEKERYKQLRTIILTNYDELDNEIKGLKLGAVDYI
                     RKPIQMASLRARIEVHVALLRAEEALRRQLDEKTITFDMLFEQAPIGIAISYSSDPEP
                     GDNSNVIINSKFEQITGRTKKELIRLGWAKITHPDDLDEDMKNFKKLKSDEIKMYSMN
                     KRFIKPDASIVWVNMTVAQLELPNDEQMSHICQVQDITERKEMEKALNESERSKSVLL
                     SHLPGLAYRCNYDCDWTMQYVSKSCYNLTGYPPESLLYNRDLSFNEIISPEYREALWN
                     EWKRVLAARQPFKYEYEIITATGEKKWVLEMGQGIYNDDGEVEALEGIILDISDRKAI
                     EDALKYNYEHDRWTGLYDREYLVSLLQKDAKLRKKSKKALIGIDLSMIQLLTANYGYQ
                     YSQNLIKKTAEALNRYCTANRILFYPRENRFVFYVLDYKDKDELVDFSNLIVDTLRSI
                     FVTERIGGGIGIVEIEQDQNDFEVDLLLSRLLIASEKSVSVFGRDFEICFYDEELEAV
                     VEREREIIKALSAIAADEDTNNDIFLQYQPIVDLKTSSVIAFEALARLRTEKLGLVPP
                     LEFIPIAEKTKLILPIGEKVIVKAFRFLNRLKKLGYDKISISLNVSAIQLLNPDFTSN
                     LFELIKEMEVNPKNICIEITESVFASDYDYINKIIMKLRDAGLYIAIDDFGKGYSSLA
                     REKGLRVDCMKIDQFFIDKLLYTDHKKAITSDIISIAHKLGHSTIAEGVEHEGQLKYL
                     KDYNCDMIQGYLI"
     gene            complement(2483..2647)
                     /locus_tag="PK768_03765"
     CDS             complement(2483..2647)
                     /locus_tag="PK768_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQA60115.1"
                     /translation="MHKIKSSSGSIGARPLQHVASSLQKALEEENEEEIEPLKESFLK
                     LLGKLFEELK"
     gene            complement(2640..5456)
                     /locus_tag="PK768_03770"
     CDS             complement(2640..5456)
                     /locus_tag="PK768_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQA60116.1"
                     /translation="MTVDQKRLLKAFRKVFQFDPSCFSFRHSSIIASKISHVLIYSII
                     IAIVLLTTITGPCYGDESSISWTEDELNFMEKDSVIRLGIDPNFVPFEFIDEDSEYRG
                     IAADYLALINEKTGLQFEIVKGLSWPEAYDMALAGHIDALPAIGKTEEREKHFLFSEP
                     YYYYKRVIVTRDTDTDISSIEDLKGFAVAVQRNSSHHSYLLSYPGINLSLYDSVEAAL
                     TAVATGEEKAFIGNLATTNYLIRSTGLTNLRFISFEAEKQQALYFAARKDWPELISIF
                     NKTLDSISEAEKLAIHNKWVELETQMDYSRIYRVLYILGAVITIVLSVSFFWIVRLQK
                     EIRRRKEIQLDLEKAKLDAEKANEFKSSFLARMSHEIRTPLNAITGMAYLLKKTELSL
                     TQSMYIDRITQASNNMLSIINDILDFSKIEAGKVRLELSSFSLDYVIQDIVNIVSYKI
                     NEKEIGFRLSKDPLLPNWFIGDSKRIQQVLLNVLNNAVKFTDEGEVSFDVRLIAKEDD
                     RYHIAFTVKDTGIGMTNEQVKKLFSPFEQGDISINRRFGGSGLGLSIVNNLVDMMGGE
                     IKVFSSPGEGTTFIIQLSLDVDKKKEEVYVKKLSAKHFKDLRTLVLEKSGANMNLIES
                     YLSSYGMHCEITSSEASAQSMLEVADGKFAKPLDLFIIDYDTPAEGGFKFVEKIRNNR
                     RIIKIPKLIMLLPMMREDLLDRLNDYGIDMGISKPIIPSILLNGILDIFNLKAVSGSQ
                     PRVEREVALTKLEDQHLVLLAEDNKTNQLIVKSLLEQVGIASIIANDGKEAVQLYKEH
                     RDNIDLILMDLHMPVMNGYEASEKIRELSAKVPIVAMTADVILGVREKCEQSGIFHYI
                     SKPFDPDNFIQTIKSIILEKEAEVDEDTVVIDRHLGIKNMGDNEELYHLVLKEFREEN
                     LDTLDRLETAVREKMICRCCTNRA"
     gene            complement(5674..6510)
                     /locus_tag="PK768_03775"
     CDS             complement(5674..6510)
                     /locus_tag="PK768_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQA60117.1"
                     /translation="MRVISWNIDSLNAALTGASARALLSRKVLDTIAESNPDIIALQE
                     TKLPSNGPNKKQLEILKDIYPEYEIVWNCSAEPALKGYAGTMFLYKHGLQPKVSFPKI
                     GAPGTMDYEGRIITLEFNDFYITQVYTPNAGENLSRLSDRQIWDIKYADYLENLDQEK
                     YVIATGDFNVAHREIDLAHPDNNRNTAGFTDEEREGFTNLLARGFTDTYRYIHGDVTG
                     VYSWWSQRARTSKINNSGWRIDYFLVSDRIADKVIRSEIVDSGPRQDHAPILLDIDLE
                     LS"
     gene            complement(6554..>7138)
                     /locus_tag="PK768_03780"
     CDS             complement(6554..>7138)
                     /locus_tag="PK768_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60118.1"
                     /translation="IEKAKSLDVKTIAPGHGPVYRTNPQKIIDDYSRFSQYAEGAGKN
                     EVTILWGSMYGMTAKAVDYVEGILQREGIKYNKIEMPQESDSEMVATVFKSAGVIIAA
                     PTYEYKLFPPVAAALDEIGRKRITGKLAFRFGSYGWSGGAEKELKEIIERNKMNWDFI
                     ESVEFEGAPKEEDLAKLETKVLELVEKMKEKVVK"
BASE COUNT         1917 a         1618 c         1288 g         2315 t
ORIGIN      
        1 gatcagatat ccctggatca tgtcacaatt ataatccttt aaatacttta gctggccttc
       61 atgttcgact ccttcagcaa ttgtgctgtg tccaagttta tgggcaatcg aaatgatgtc
      121 gctggttatt gctttcttat ggtctgtata caacaattta tcaataaaaa actggtctat
      181 tttcatgcaa tcaactctta agcccttttc tctggcaaga gatgaatagc ctttcccaaa
      241 gtcatctatt gctatgtaaa gacctgcgtc ccgtagtttc ataataattt tatttatgta
      301 atcataatcg gaagcaaaaa ccgattcagt gatctctata caaatgttct tcggattaac
      361 ctccatctcc tttattaact caaacaggtt acttgtaaaa tcaggattca acaactgaat
      421 ggcagaaacg tttaaagata tgctgatctt atcatatcca agctttttga gcctgtttaa
      481 aaagcgaaat gcttttacaa ttactttttc accaattgga agaataagct ttgttttctc
      541 tgcaatcggt atgaattcca ggggtgggac aagtccgagc ttttccgtcc tcaatctggc
      601 cagtgcttca aaagcgataa ctgaacttgt ttttaaatcc acaatcggtt gatattgcaa
      661 aaatatgtcg ttatttgtat cttcgtcagc tgctatagcg cttaaagctt taataatttc
      721 cctttcacgt tcaactacag cttccagctc ttcatcataa aaacatattt caaagtctct
      781 accaaaaacg cttacagact tctccgaggc aatgagaagc ctacttaaaa gcaaatcaac
      841 ttcgaaatca ttttggtcct gctcaatttc tacaatccct atcccgccac ctattctttc
      901 ggttacaaat atggacctca acgtatctac aataaggtta ctgaaatcaa caagttcgtc
      961 tttgtcttta tagtcgagga catagaaaac aaagcgattc tctcttgggt agaacaagat
     1021 acgattagcg gtacaatacc ggttaagagc ctcagctgtc ttttttatca gattctgtga
     1081 gtactggtat ccgtaatttg ctgttaacaa ttgaatcatg cttaagtcaa tacctatgag
     1141 cgcttttttc gactttttcc ttagcttggc atccttttga agtaaagaca ccaaatactc
     1201 gcgatcgtaa agtccagtcc atctatcgtg ttcataattg tactttaagg catcttcgat
     1261 cgcttttcga tccgatatat caagaattat tccttccagt gcttcaactt cgccatcatc
     1321 gttgtatatg ccttgaccca tctcaagaac ccacttcttc tcacccgtgg cagttataat
     1381 ctcatactca tatttaaagg gctgtcttgc cgcaaggact cgcttccatt cattccacaa
     1441 tgcttcgcgg tattccggag aaataatttc attaaaggaa aggtctctat tgtatagcaa
     1501 gctttccggt ggataacctg tcaggttata acaactctta gatacatatt gcattgtcca
     1561 gtcgcaatcg tagttacacc tgtatgcaag gcccggaagg tgggaaagta aaaccgattt
     1621 gcttcgctca ctctcgttta gcgctttttc catctccttg cgctcggtga tgtcctggac
     1681 ctggcagata tggctcatct gctcatcatt cggtagttca agctgagcca ctgtcatatt
     1741 tacccaaacg atcgaagcat caggtttaat gaaccgttta ttcattgagt acatcttgat
     1801 ttcgtcggat ttgagcttct taaaattttt catgtcttca tccaaatcat ccggatgagt
     1861 tatctttgcc cagcctaatc gaatgagctc tttttttgtc cttccagtaa tttgttcgaa
     1921 ctttgaattg attattacat tggaattatc accgggctcc ggatcagaac tatacgaaat
     1981 tgcaatgccg ataggggctt gttcaaacag catgtcaaaa gttattgtct tttcatccaa
     2041 ctgccgcctc aaagcttcct ctgcacgcaa tagtgcaaca tggacctcaa ttcttgcccg
     2101 tagggaagcc atttgaatag gttttcgaat gtagtctaca gccccaagct tcaagccctt
     2161 gatttcattg tccaactcat catagttagt caaaattata gtgcgcagct gcttataccg
     2221 ttccttttct tttagatact ctagaacttg aaagccgtcc attttaggca tttttagatc
     2281 cagtatgagc aaattgatcc cgtcatgctc ctctagcatg cgaatggctt caacgccgtc
     2341 ggaagctgtg aaaatgatat aatcgctcag catattttta atgataaacc tgtcagttgc
     2401 cgagtcatca actaccaata tcttaacttt catagcaaat ccaccttctt aaaaacacat
     2461 ttattctcgc attaacacaa gtttatttaa gttcctcaaa cagttttccg agtaatttca
     2521 aaaagctctc cttaagcggc tctatttctt cttcattttc ttcttccaag gctttctgta
     2581 atgatgaagc tacatgctgg agtggtctgg caccaatgct tccggaactg ctctttattt
     2641 tatgcacgat ttgtgcagca tcggcatatc attttttccc gaaccgccgt ttcaagcctg
     2701 tccaaggtat ccagattttc ttcacggaat tctttcaata ctaggtgata gagttcttcg
     2761 ttatctccca tattttttat gcccaaatgc cggtcaataa ccacagtatc ctcatcaact
     2821 tcagcttctt tttctagaat aatacttttg atggtttgaa taaagttatc tgggtcaaag
     2881 ggcttgctga tataatggaa gataccgctt tgctcacatt tttcgcgaac acctaatatg
     2941 acatctgctg tcattgcaac tatagggact ttggctgaaa gctcccggat tttttccgat
     3001 gcctcataac cgttcatgac aggcatgtga aggtccatta atatcaaatc aatgttgtct
     3061 ctatgctctt tatacagctg cacagcttcc tttccatcgt tggcaataat agaagctatc
     3121 cctacttgct ccaagagtga ctttacgatc agctggttag ttttattatc ctccgccaac
     3181 aatacaagat gctgatcttc aagtttggtc agagctactt ctctctctac tctaggctgt
     3241 gacccggaaa ccgcctttag gttgaaaatg tccagaatcc cgttgagcaa aatggagggg
     3301 attatcggct tgctgatgcc catatcaata ccatagtcgt tgagtctatc gagaaggtct
     3361 tcccgcatca tcggtaaaag cattatgagt ttcggaattt taattatcct tctattgttg
     3421 cgtattttct ccacaaattt gaaaccgcct tctgcagggg tatcgtaatc gataataaac
     3481 aaatccaagg gcttggcaaa ttttccgtct gctacttcga gcatgctttg ggcactcgct
     3541 tccgaacttg taatctcaca gtgcattcca tagctgctca agtagctttc aatcagattc
     3601 atgtttgcac ctgatttctc caacaccagc gttctgagat ctttgaagtg tttggctgat
     3661 aattttttta catagacttc ttccttcttt ttatcgacat ccaatgacag ttgaataatg
     3721 aaggtagtgc cttctccggg gctactaaac acctttattt ctccgcccat catatctaca
     3781 aggttgttca ctattgacaa gcctaaaccc gaaccaccaa aacggcgatt gatactgatg
     3841 tcaccctgct caaatggtga aaaaagcttt ttcacctgtt catttgtcat ccctattccg
     3901 gtgtccttaa cggtaaaagc aatgtgatac ctatcatcct ctttagctat cagtcttaca
     3961 tcgaatgaca cttcgccctc atcggtgaat ttgactgcat tgttcagaac gttgagcagt
     4021 acctgctgaa tccttttgga atcaccgata aaccaatttg gaagaagagg gtcttttgac
     4081 agtcgaaaac cgatctcctt ttcattgatt ttatatgaaa caatattgac gatatcctga
     4141 atcacataat ccaaactgaa agagctgagc tcaagccgca ctttcccagc ttctatttta
     4201 gagaaatcaa gtatatcgtt tattatgctc agcatgttat ttgatgcttg agtgatcctg
     4261 tcgatgtaca tactttgcgt taaactaagc tcggtctttt tcaacaggta tgccataccc
     4321 gttatagcgt taagaggggt tcttatctcg tgggacatcc ttgccaagaa actcgatttg
     4381 aattcatttg ccttttcggc atctaacttt gctttttcca gatcaagctg gatctctttt
     4441 cttctccgaa tctccttttg cagtctcaca atccaaaaaa atgatacgga caaaacaatt
     4501 gtgattacgg cacccaggat gtacaaaacg cgataaatac ggctgtagtc catctgagtt
     4561 tccaattcca cccacttgtt gtggatggca agtttttcgg cctcggaaat tgaatccaag
     4621 gttttattaa agatgcttat aagttcaggc cagtctttcc gagcagcaaa atatagggct
     4681 tgttgttttt cggcttcaaa agatatgaat ctgagatttg tcaaacccgt tgaacgtatt
     4741 aggtaatttg ttgttgccag gttcccgata aaagcttttt cttctccagt tgctactgca
     4801 gtaagcgcag cttcgacgga gtcatagagg cttaggttta taccgggata tgacagcagg
     4861 tagctgtggt gcgaactgtt gcgctgcaca gctacggcaa agccttttaa atcctctatg
     4921 ctcgatatat cggtatccgt atcgcgggtg acgatcactc tcttgtagta gtaatagggt
     4981 tcagaaaaga gaaagtgttt ttcccgctct tctgttttac cgatcgcagg cagtgcgtcg
     5041 atatggcctg caagggccat atcataagct tctggccagc tcaagccctt tacaatttca
     5101 aattgcaaac ctgtcttttc gttaattaag gcgagatagt cagctgcaat tcccctgtac
     5161 tcgctgtctt catcgataaa ctcaaatggc acaaagttcg ggtcgatgcc aaggcgaatt
     5221 accgaatctt tttccataaa gttcagctca tcttctgtcc aacttatact actttcgtca
     5281 ccatagcaag gacctgtaat tgtggttaac aggacgatag ctataatgat tgaatatatc
     5341 aggacatgac ttattttcga ggctatgata gaggaatgtc gaaaactgaa acacgacggg
     5401 tcaaattgga atactttgcg aaaggccttt aataatctct tttgatcgac ggtcatagca
     5461 tgaccccatc tctagactaa actcgaatgg aataatctag aagtattatg tcacattttg
     5521 tcataattgt caattaagta ttatttgtgt gattttgtaa ttatttttgt acatcaagac
     5581 atagaataca tgggactata aaaatacagg gttttcggaa acagccaaaa agcaattctg
     5641 cagcaagtct cgaaaaccct gtatattatt tgtttaggaa agctctaagt caatatccaa
     5701 taaaataggc gcgtggtctt gtcttggacc ggaatcaacg atttcagaac gtatgacctt
     5761 atctgcaatc ctatcgctca ctaaaaaata atcgattctc cagccggaat tatttatttt
     5821 actcgtcctt gcacgctgcg accaccagga ataaacaccg gtgacatccc cgtgtatata
     5881 acgatatgtg tctgtaaaac ctcttgcaag aagattggta aaaccttcac gctcttcatc
     5941 ggtaaagccg gctgtattgc ggttgttatc gggatgggcc aagtcaattt ctctatgggc
     6001 gacattgaaa tcgccggtag ctataacgta tttttcttga tccaggtttt ccagatagtc
     6061 ggcatactta atgtcccaaa tttgccgatc actcaagcgg cttaagtttt cccctgcgtt
     6121 gggggtgtat acctgtgtta tatagaaatc attaaactct aaggtgataa tcctgccttc
     6181 ataatccatg gtgccaggcg caccgatttt aggaaaactt actttcggtt gtaaaccatg
     6241 cttatataag aacatggtgc cggcataacc ctttagcgca ggttctgccg aacagttcca
     6301 cacaatctca tattcaggat atatatcttt taatatttcc aactgctttt tattggggcc
     6361 attgctcggt aactttgtct cctgcaaggc aataatatcc ggattacttt cagcaatggt
     6421 atccaatact ttgcgactca ataatgccct tgccgaagca cctgtcaatg cagcatttaa
     6481 cgaatcaata ttccatgaaa taaccctcaa agtttctcct cccctgagaa attgatatgt
     6541 attaaataat gtgttatttt actacctttt ccttcatctt ttctacaagc tcaagtactt
     6601 ttgtttcaag cttggctagg tcttcttctt ttggcgcccc ttcaaattca acggactcta
     6661 taaagtccca attcatctta tttctctcaa ttatttcttt tagctccttc tctgcaccac
     6721 ccgaccatcc gtatgagcca aacctaaatg cgagttttcc ggtaatcctc tttcttccta
     6781 tctcatctaa agctgctgcc accggcggaa aaagcttgta ctcatacgta ggagcagcaa
     6841 tgataacccc cgcagactta aaaacggttg ccaccatttc actgtcactt tcctgcggca
     6901 tttcaatctt gttgtactta atgccttctc tttgaagtat accttcaacg taatcaacgg
     6961 cttttgcagt cattccgtac atggagcccc aaagtatagt aacttcgttt ttccctgctc
     7021 cttctgcgta ttgggaaaac ctggagtagt catcaatgat cttttgagga tttgttctgt
     7081 aaacggggcc gtggccaggc gcaatagttt ttacgtcgag ggactttgct ttttcaat
//
LOCUS       DAONUO010000070         7136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7950_length_7136_cov_3.864708, whole genome shotgun sequence.
ACCESSION   DAONUO010000070 DAONUO010000000
VERSION     DAONUO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7950_length_7136_cov_3.864708"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK768_03785"
     CDS             <1..228
                     /locus_tag="PK768_03785"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HQA60119.1"
                     /translation="EEVINAGVAIIATVHGSSLEEIKKRPTIRKLINRLFFDRYIILS
                     KNLGVGKIEAILDSATGRNLIKIRREEKMCG"
     gene            219..740
                     /gene="spoIIIAB"
                     /locus_tag="PK768_03790"
     CDS             219..740
                     /gene="spoIIIAB"
                     /locus_tag="PK768_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein SpoIIIAB"
                     /protein_id="HQA60120.1"
                     /translation="MWLKLVGGMMVVLSSSMIGFIIAGTYKKRPNTLRNLQAALSMLE
                     SEINYGHLPLPQALKSISRKCDKDVAELFARAAQYLSSRNGLTADEAWEKSLKEFFFN
                     SYIAENDYEILIAFGKYLGSTDKQDQIKNIRLAMNNLRQQELSSIQEKEKNEKMWRYL
                     GVLSGIMVVLLLY"
     gene            757..954
                     /gene="spoIIIAC"
                     /locus_tag="PK768_03795"
     CDS             757..954
                     /gene="spoIIIAC"
                     /locus_tag="PK768_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HQA60121.1"
                     /translation="MEVDILFKIAAIGIVISILSKVLDEAGRKEQAQMTTLVGVVIVL
                     MMVIQLVSQLFSNVKSMFQLY"
     gene            966..1352
                     /gene="spoIIIAD"
                     /locus_tag="PK768_03800"
     CDS             966..1352
                     /gene="spoIIIAD"
                     /locus_tag="PK768_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HQA60122.1"
                     /translation="MEIIQIVGLCIVTTILVVLLREDRPEIALQMSIIVGAIIFLRMI
                     DQILAVIEYLRELSLRANINNLYISTVFKIIGIAYIAEFGSQICKDAGSHAIASKIEF
                     GAKILILVLSLPLLMAVMDMVIRILP"
     gene            1402..2574
                     /gene="spoIIIAE"
                     /locus_tag="PK768_03805"
     CDS             1402..2574
                     /gene="spoIIIAE"
                     /locus_tag="PK768_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HQA60123.1"
                     /translation="MKIVYFVVITLIISLFFAANTALAQDNINEEILKKQYELIDKAE
                     FDLFIEQLNSTYRDYIPQYGLDDLIKAFRGQKPYDLKALITGIAGYFFHELSANMSLL
                     AELIIISIICAVMYNVQSAFENDNIGKVTYAFTFLLLIAIAIQSFDIALGVGRDTIDK
                     MVSVIQALLPVILPLLASIGGLTSVAIFNPVIFLGVSIASTWIRNILLPIIYFVAALG
                     IISSISDRFHVSALSSLLKQVCIFLLGLFLSAFLGILVVQGAAASTVDGITIRTAKFA
                     SKNFIPIVGSIFSDTIDTVVGCSLILKNAIGFTGLVVIFLTILFPVIKILAMVFIYRL
                     AGAIIQPLGVEPMVKCLNEIAGNLTFIAVTVSSVALMFFVAVTVIIASGNISLMMR"
     gene            2579..3208
                     /gene="spoIIIAF"
                     /locus_tag="PK768_03810"
     CDS             2579..3208
                     /gene="spoIIIAF"
                     /locus_tag="PK768_03810"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HQA60124.1"
                     /translation="MLAAVSQWVRQIVMVVMFAAFVDFFIPEKSFSRYVKVFLGLLVM
                     IVIMSPVMSLFHRDFSIDEIAFVYDDPIDYRSINSKSHILSENNDKIKIAEYKANIEK
                     YLFQKIAEITSHDLRDVSVKLVEDFSSPDFGQISEIVITLNKNPPGQLADGNVKQEKI
                     SIEKVKIDNKPLGSNVLSEMTKEEFKEIVNYLAASFNIPEEKIYISLED"
     gene            3213..3806
                     /gene="spoIIIAG"
                     /locus_tag="PK768_03815"
     CDS             3213..3806
                     /gene="spoIIIAG"
                     /locus_tag="PK768_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HQA60125.1"
                     /translation="MKSQLDIKSILEWIKNPKNKVVSKFIFIFTLGLLLMSLSKMFSD
                     TNYKHAKNDSKELQQIPRQSIENLSYENRLEKQLSELLGQVEGVGDVNVMIVLEDETL
                     IEPAFNVIDTEKTSEEKDNEGGVRTIVEKQRNQQMVLLRKNGEEEAMVLKKTAPRIKG
                     ILIIADGASSSKIKEKIIKSTATLLDIPTYRISVLAK"
     gene            3880..4425
                     /locus_tag="PK768_03820"
     CDS             3880..4425
                     /locus_tag="PK768_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HQA60126.1"
                     /translation="MSFVIKKRTLLIIAFALILLLSAYMMFLGNFPKVVYEKQLDSGE
                     LEKVSISAIDDTQEISAVANLENDFFIEYKLERDRLRGQEADYLRELINNPNASTESK
                     EKAQQELIALSQKVEKEMLVENLIRAKGFEDAVIFFSDDHINVVVKTDGLMQKDIAQI
                     TDVVTKTTGVSVDKITIIERK"
     gene            4502..4879
                     /locus_tag="PK768_03825"
     CDS             4502..4879
                     /locus_tag="PK768_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQA60127.1"
                     /translation="MSNADINNDKGSIKIADDVVGVIAGLAATEVEGVAGMSGGIVGG
                     IAEMLGRKNLSKGVKVEVSDKEAKVDLYMIINYGVRIPDVAWNVQENVKKAIENMTGL
                     SVTEVNIHIQGVSFGPAKEGEGK"
     gene            4942..5475
                     /gene="amaP"
                     /locus_tag="PK768_03830"
     CDS             4942..5475
                     /gene="amaP"
                     /locus_tag="PK768_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HQA60128.1"
                     /translation="MKFLDKLLIVLLSLLATCVSLFLIVISARVVSQQYLSIYLSMVY
                     GKWETGALGIVLLIFSLFVLVYVLKPRKLPEAVVSDSELGKVCITLGAVESLVQKVIR
                     DIQDVTESRIYIKRQEDGVSITLKITVNYDVIIPELASEMQGTIRDYVESTAGIKVNN
                     VLIRVSNVSNQKAKAGK"
     gene            5479..5706
                     /locus_tag="PK768_03835"
     CDS             5479..5706
                     /locus_tag="PK768_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HQA60129.1"
                     /translation="MNAVDYLRELWHEHRREIIGGIAGLIIGIIIITLGFFKALFVIF
                     CTLLGYYIGKNVDRKEDIRSLLDKILPPSNR"
     gene            5719..6120
                     /gene="nusB"
                     /locus_tag="PK768_03840"
     CDS             5719..6120
                     /gene="nusB"
                     /locus_tag="PK768_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQA60130.1"
                     /translation="MSRKIAREQAFKILFAIDVGGNTIEEASEPMIDSLNDDQKSFIF
                     REVEGVLNNLSNIDKHINRYSEDWTVGRMASADRNILRLAVYEILYCDDIPVSVSINE
                     AIELAKKYGDEKSYRFINGLLGSIAKDCLPR"
     gene            6149..>7136
                     /gene="xseA"
                     /locus_tag="PK768_03845"
     CDS             6149..>7136
                     /gene="xseA"
                     /locus_tag="PK768_03845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQA60131.1"
                     /translation="MKPVFTVSEVNNIIKNLLDNNEILRQVYVRGEISNFKHHNMSGH
                     MYFTLKDEMSQIRCVMFKNSNRLLSFMPENGMKVIAFGFISVFNRDGQYQLYVEALEP
                     DGIGALHIAFERLKMRLEQEGLFAQERKLPIPFLPKKIALVTSPTGAAIRDLLTVIRR
                     RFPNVDIVIAPVLVQGEGAADDICSAISQLNSLQGLDVIIVGRGGGSIEELWAFNEER
                     VARAIAESKVPVISAVGHETDYTIADFVADKRAPTPSAAGEIVVPEKRTLKNELRHIS
                     LRLGNGFYNYIGEKRQKLEYLKSAQVYKKPRTYVINCRLVVDELFRRLIKETN"
BASE COUNT         2372 a         1074 c         1542 g         2148 t
ORIGIN      
        1 gaagaagtca taaatgcagg tgttgccatt atcgctacgg tgcacggctc aagtcttgaa
       61 gaaataaaaa agaggcctac cattcgaaaa ttgataaaca gattattttt tgatagatac
      121 atcatcctaa gtaagaattt aggagtggga aaaatagaag ccatactgga tagcgcgact
      181 ggaaggaatt tgataaaaat caggagggag gaaaagatgt gtggctgaaa cttgtgggag
      241 gaatgatggt tgttctatct tctagcatga taggctttat tatcgctggt acttataaaa
      301 aaagacctaa tacattgaga aatttacaag cagcactttc catgcttgaa tctgaaataa
      361 actatggcca tttaccgctt ccacaagcac taaagagcat cagcaggaag tgtgataagg
      421 atgtggcgga actttttgct cgagcagcac agtatctttc ttccagaaat ggtttgactg
      481 ccgatgaagc atgggaaaag tcgctaaagg aattcttttt taactcttat atagcagaaa
      541 atgattatga aatattaata gcattcggga aatacctggg ctcaacggac aaacaggacc
      601 aaattaaaaa tataagattg gcaatgaaca atttaaggca acaagaactc agctcaattc
      661 aggaaaagga gaagaatgag aaaatgtgga gatatttagg tgtattgtcg ggcatcatgg
      721 ttgtcctctt actatattag caagagaggt gttagtgtgg aagtagatat tctattcaaa
      781 atagctgcga tcggcatcgt catttcaatt ttgagcaaag tacttgatga agcgggcagg
      841 aaggagcaag ctcaaatgac tactcttgtt ggtgtcgtaa tagttttaat gatggtaata
      901 caattggtca gtcagttatt ttcgaacgtc aaaagcatgt tccaacttta ttaggaggac
      961 ttttcatgga aataatacaa attgtcggcc tctgcattgt tacaacaata cttgtggtgc
     1021 ttcttcggga ggacaggccg gaaatcgctt tgcaaatgag tataattgtg ggtgcaataa
     1081 tcttcctgcg catgatagac caaattctag ctgttattga atatttaagg gagttatcat
     1141 taagagccaa tattaacaat ttgtatatca gtactgtttt taaaattata ggaatagctt
     1201 atatagctga atttggctca caaatatgca aagatgctgg ttcgcacgca atagcgtcaa
     1261 agatagaatt tggtgcaaag atactcatat tagttttatc actcccgctt ttaatggcag
     1321 tgatggatat ggttatcagg atacttcctt gaagacgtgc tcggaaaacc aactatattg
     1381 tacaaggagg tttagtattt tttgaaaata gtatattttg ttgtaattac tttaataata
     1441 tctttgttct ttgctgcaaa cactgctttg gcccaggaca acataaatga agaaatacta
     1501 aaaaaacaat atgaactaat tgataaggca gaatttgatt tattcattga acaattaaat
     1561 tcaacttata gagattacat acctcagtat ggtttggatg atttgataaa agcctttaga
     1621 ggtcaaaaac cttacgactt aaaagcttta ataacgggca tagcaggata ctttttccat
     1681 gaattatctg caaacatgag cttattagct gaactcataa tcatatctat aatttgcgcg
     1741 gtgatgtata atgtgcaaag cgcctttgaa aatgataaca tcggaaaggt aacatatgca
     1801 tttacatttt tactgctgat agcaatagct attcaaagtt ttgacattgc tttgggagta
     1861 ggtagggaca caatagataa aatggtaagt gtcattcaag cgctgttgcc ggtaattcta
     1921 ccgttgctcg cttctatagg cggtttaact tcagttgcta tcttcaaccc agtaatcttt
     1981 ttaggtgttt ctatagcaag tacatggatt agaaatattc tgcttcctat tatttatttt
     2041 gtagctgcat taggtattat aagcagcatt tctgacaggt ttcatgtgtc cgcgttatca
     2101 tctctattga aacaagtatg tatttttttg cttggtcttt tcctcagcgc ttttttaggc
     2161 attttagtgg ttcaaggtgc tgctgcatca acggtagatg gaataacaat aagaactgcc
     2221 aaatttgctt caaaaaactt tattcccata gtcggcagta ttttttcgga tacgatagac
     2281 acggttgtag gctgttcttt aatactaaaa aatgccattg gatttaccgg cctggttgta
     2341 atatttttaa ctattttatt tccggtgatt aaaatattgg ctatggtgtt catatatagg
     2401 ttagcagggg ctataattca gcctttagga gttgaaccaa tggtcaaatg tttaaatgaa
     2461 atagcaggca atttaacttt tattgctgtt acagtaagct ctgttgcatt aatgtttttt
     2521 gtagctgtaa cggtaatcat tgcgtccggc aacattagcc ttatgatgag gtgatgcaat
     2581 gcttgctgca gtaagccaat gggttagaca aatagtgatg gtggtcatgt ttgcggcctt
     2641 tgttgatttt tttataccgg aaaaaagctt ttcaaggtat gtaaaagttt ttttaggtct
     2701 tttagtgatg atagtaataa tgagtccggt gatgtctctt tttcataggg atttctcaat
     2761 tgacgaaatt gcttttgtat atgatgatcc tatagattac agaagtatta attcaaagtc
     2821 ccacatctta agtgaaaaca acgataaaat aaagattgca gagtataaag caaatattga
     2881 gaagtattta tttcaaaaaa tagccgaaat aacttctcat gaccttcgag atgtatctgt
     2941 taaactagtt gaggattttt ctagtcctga ctttgggcaa atatctgaaa ttgtaataac
     3001 tttgaataaa aatccaccag gtcagcttgc tgacggaaat gtcaaacaag aaaaaatatc
     3061 cattgagaaa gtaaagatag ataataaacc attgggttct aacgtccttt ccgaaatgac
     3121 aaaagaagag tttaaagaaa ttgtaaacta cttggctgca agctttaata ttcccgaaga
     3181 gaaaatatat ataagtttgg aggattgagt tgatgaaatc acaactagac ataaagtcga
     3241 ttcttgaatg gataaagaat cccaagaaca aggtcgtttc caaatttatt tttattttta
     3301 cattgggatt gttgctgatg tccttaagca aaatgttttc cgatactaat tacaagcacg
     3361 cgaaaaacga ctctaaagag ttacaacaaa ttccccgtca gtcaatagaa aacctgtctt
     3421 atgagaatag gctggaaaaa caactgtcag aattgttggg ccaagtagaa ggcgtaggtg
     3481 atgttaatgt gatgattgtc ttagaggatg agacattgat tgaaccggca tttaatgtta
     3541 ttgacaccga aaaaacatct gaggaaaaag acaatgaagg aggtgttagg acaattgtag
     3601 aaaagcaaag aaatcaacag atggtcctgc tcagaaaaaa tggtgaggaa gaggctatgg
     3661 ttttaaaaaa aactgcccct agaataaaag ggatactcat cattgccgat ggagcctcat
     3721 catcaaaaat caaagaaaag attattaaat ccacagccac actattagac atacccactt
     3781 atagaattag tgttttggca aaataggcta tataccagcg agataccgct tttttatgtt
     3841 ataggcttta atatttttta atattgggag gggaaagggg tgtcatttgt gattaagaaa
     3901 agaaccctgt taataatcgc atttgcttta atacttttat tgtctgcata catgatgttt
     3961 ttaggtaact ttccaaaagt ggtgtacgaa aaacagctgg attctggtga attggaaaag
     4021 gtatctattt ctgcaattga tgatacacaa gagatttcgg ctgtagcaaa tctggaaaat
     4081 gattttttta tagagtataa attggaaaga gatcgattga ggggccagga agctgattat
     4141 ttgagggaac taataaacaa tcccaatgcc agtacagaat ctaaagaaaa agcccagcaa
     4201 gaattaatag cgctttccca gaaagtagag aaggaaatgc ttgtagagaa tctcataagg
     4261 gcgaagggct ttgaagatgc agtgatcttc ttttctgatg accacatcaa tgtagtagta
     4321 aaaactgatg gtcttatgca aaaagatatt gctcagatta cagatgttgt tacaaaaaca
     4381 accggtgtgt cggtggacaa aattaccata atagaaagaa aataggccac attaattgta
     4441 taaatgtatt tattttgcta taatatcgtt aggtttatta ccatccggaa ggagaaggaa
     4501 aatgagtaat gcggacatta acaatgacaa gggttcaata aaaattgctg atgatgtcgt
     4561 aggtgtaatt gcgggccttg ctgctacgga agttgagggg gtagcaggca tgagtggagg
     4621 catcgttggt ggaattgccg agatgttagg gcgcaaaaat ttatcaaaag gtgttaaggt
     4681 tgaggttagc gataaagaag ctaaagtaga tctgtacatg attataaact acggtgttcg
     4741 aataccagat gtagcctgga atgttcaaga aaatgtgaaa aaagcaatag aaaatatgac
     4801 cggcctgtct gttactgaag ttaatataca tatccaaggt gtcagcttcg gtcctgctaa
     4861 ggaaggagaa ggcaaataac ctaatacccc ctgataatga cgtgttcagg gggttaattt
     4921 cctttagaaa ggagcttact tatgaagttt ttggacaaac tgttaattgt tcttttatcc
     4981 ctccttgcaa cctgtgtgtc cttattcctt attgttatat ctgctagagt tgttagtcag
     5041 caatatctta gtatttactt atccatggtc tacggaaaat gggaaacagg tgccctcggt
     5101 attgtcttgt tgatatttag tttattcgtt ctagtctacg tcttaaagcc aaggaaactg
     5161 ccagaagctg tggtcagtga cagtgagctg ggaaaggttt gtattacctt gggtgctgtt
     5221 gaaagtttag ttcaaaaagt gatacgggat attcaagacg taacagaatc gaggatttac
     5281 ataaaaagac aagaagatgg ggtgtcaata accctaaaaa ttactgtaaa ttacgatgtt
     5341 ataatcccgg aactagcttc tgaaatgcaa gggacaatta gagattatgt tgaatctact
     5401 gcaggaatta aagttaataa cgtgttaata agagttagca atgtttcaaa tcaaaaagca
     5461 aaagcaggaa aataaggggt gaatgcggtg gactatttgc gagagctgtg gcatgagcat
     5521 agaagggaaa taattggcgg gattgcagga cttataattg gcattattat catcacctta
     5581 gggtttttca aagctttgtt tgtcattttc tgcacactat taggatatta tataggtaaa
     5641 aatgtagata gaaaagaaga tattcgcagt ttacttgata aaatattacc tccaagtaat
     5701 agataaggag tgcttttctt gagtaggaaa attgccagag aacaagcatt taagattctt
     5761 tttgctatag atgttggtgg caatacaatt gaagaagcat cagagcctat gattgactcc
     5821 ctaaatgatg atcaaaaaag ttttatattt agagaagtag agggagtttt gaacaattta
     5881 tcaaatattg ataagcatat aaacaggtat tcagaagatt ggacggttgg ccgaatggca
     5941 tctgcagata gaaatatact tcgccttgca gtgtacgaaa tactgtactg tgatgatata
     6001 cctgtcagcg tttccataaa tgaagcaatt gaacttgcaa aaaaatacgg agacgaaaag
     6061 tcatatagat tcataaacgg attgttaggg tcaatcgcaa aagattgctt gccaagatga
     6121 aaaacgtagt atcttggggg agaaagtttt gaagccagtt tttaccgtta gtgaagttaa
     6181 caatattata aagaatcttt tagataacaa tgaaatttta agacaggttt acgttcgagg
     6241 agaaatatca aattttaaac accacaatat gtccggccat atgtacttta cactaaaaga
     6301 tgagatgtca caaattcgct gtgttatgtt taaaaacagc aataggctac tttcttttat
     6361 gcccgagaac ggcatgaagg taattgcttt cggttttata agtgtattta acagggacgg
     6421 ccaatatcaa ttgtatgttg aagctttaga acctgatggt ataggagccc tgcatattgc
     6481 ctttgaaagg cttaaaatgc gcttggaaca ggaaggcctt tttgcacaag agagaaaact
     6541 gccgataccc tttttaccca aaaaaatagc tctggtgact tcccctaccg gtgcagcaat
     6601 aagggactta ttgaccgtga taagaaggcg ctttcccaat gtggatatag taattgcgcc
     6661 ggtactggtt cagggtgagg gagctgccga tgacatatgc tctgccattt cacagctcaa
     6721 cagtttacag ggtttagacg ttataattgt aggcagaggc ggcggatcca ttgaggaact
     6781 ttgggccttt aacgaggaaa gggtagccag ggccattgcg gaatctaaag tgcccgtcat
     6841 ctcagccgtt ggccatgaaa cggattatac gattgctgat tttgttgcag acaaaagggc
     6901 acctactcct tcggctgcag gtgaaattgt agtgccggaa aagcgcaccc taaaaaatga
     6961 attaaggcac atcagtttaa ggttaggtaa tggattttat aattacattg gagaaaaaag
     7021 gcaaaaactc gaatatttaa aatcggcaca ggtatataaa aaaccgcgaa catatgtgat
     7081 taattgtcga ctggtagtag acgaattatt cagaaggcta ataaaagaga ccaatt
//
LOCUS       DAONUO010000071         7107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7987_length_7107_cov_4.265031, whole genome shotgun sequence.
ACCESSION   DAONUO010000071 DAONUO010000000
VERSION     DAONUO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7987_length_7107_cov_4.265031"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1837)
                     /locus_tag="PK768_03850"
     CDS             complement(<1..1837)
                     /locus_tag="PK768_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HQA60132.1"
                     /translation="MTVPQQLIKRRLIILLLFSLIFSFLLLGRVFWIQFVRGDELKER
                     ALNQWTSDVPVEPKRGTVYDRNKNPLAISATVDTVVASPPDIKDIETTANLLSTALNM
                     DREELEETLREAKNKKKGAIYIKRKITEEESETVRNLKLSGIYFTKEHKRFYPERNLC
                     SHVLGFTGIDSQGLDGIELVYEKYLKGTPGRIVSEKDALSRKLPFGVDKYIPPEDGLD
                     LVLTIDKIIQHFAERELEKALAEHNAKRGTIIVMDPKTGEILAMTNKPDYDPNNYGEF
                     SQILWRNAAVSDVYEPGSTFKIVTASAALEEGVARPEDKFYDPGYAMVSGVRIGCWYH
                     GGHGSQTFIQLLENSCNPGFVELGMKLGKDRFMKYIKGFGFGEVTGIDLPGEARGILD
                     PSKVGPVELATITFGQGISVTPVQLITALSTIANDGKLVQPHVAKAIVDKEGNTVQEF
                     SPAPLRQVVSYETAQEMKKILESVVTNGTGGRGKVEGFRVAGKTGTAEKYSPGKYVVS
                     YMGFAPVDDPKLAILVIIDEPTGGLIYGGTIAGPVFQKVMADSLNYLGIEPNIEQVEE
                     REHVAVPDLRNLYVEDARKILMQNGLSVRIEGEGYVVTEQVPMPGA"
     gene            complement(1861..2223)
                     /gene="ftsL"
                     /locus_tag="PK768_03855"
     CDS             complement(1861..2223)
                     /gene="ftsL"
                     /locus_tag="PK768_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HQA60133.1"
                     /translation="MVVAERELQRRSFYREEAYQREIHRRQQKSHSNHKLRYILRLAV
                     IALLLFAVLYRFSVITEYQYRIEKLQSEIEDISMQNERLRVEIASLKSIARIEDIAIN
                     KLNMKEPDNRQFIYLNSK"
     gene            complement(2225..3187)
                     /gene="rsmH"
                     /locus_tag="PK768_03860"
     CDS             complement(2225..3187)
                     /gene="rsmH"
                     /locus_tag="PK768_03860"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQA60134.1"
                     /translation="MNYHHVPILLNEVIEILNPKPGGVYVDATLGGGGHFRELIKKAE
                     YSGTFIGIDQDEAAISNARQIFSKYQGDIRLIHDNFSNVKKIIRENNISAIDGILFDL
                     GVSSHQLDKGSRGFSYMQDAVLDMRMDKRNPLTAKDVVNEMSREELKRIIREYGEEPW
                     AGRIADFICERRKQQKIITTGQLVDIIKDAIPARARRKGGHPARKTFQALRIYVNKEL
                     EILESSIKDAVELLKPAGRICVITFHSLEDRIVKDAFKFLASDCICPKNALICTCNHF
                     KTIKILTSKPIYPSQGEIEVNPRARSAKLRAAEKLSVLKPRKGE"
     gene            complement(3199..3627)
                     /gene="mraZ"
                     /locus_tag="PK768_03865"
     CDS             complement(3199..3627)
                     /gene="mraZ"
                     /locus_tag="PK768_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQA60135.1"
                     /translation="MFIGQFQHSLDQKGRLIIPSKFREDLGQSFVLTKGLDGCLFVYP
                     RDEWAVLEQKLKTLPFTQKDARAFIRFFFAGAVEAEMDKQGRILIPPQLREHAGIERD
                     VVIIGVSNRVEIWSQEQWEAYSVKAAESYEDIAETLDLGI"
     gene            complement(3795..5066)
                     /locus_tag="PK768_03870"
     CDS             complement(3795..5066)
                     /locus_tag="PK768_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQA60136.1"
                     /translation="MESCLAERLFSKETQSGFKVYVLPKKDYNKIFAMYSTNYGSIDS
                     EFIVPGTGEHLKVPEGIAHFLEHKMFEMEYGNVFDKFSELGASPNAYTSYTNTTYLFS
                     GTSHFRESLKLLLEFVESPYFTEESVEKEKGIITQELRMYEDEPEWQLLLNLLKCLYH
                     KHPVRIDIGGTVESIQNIDVETLYKCYHTFYHPSNMILFVIGCIDPEEVFELVEKNEA
                     SKGLSPQAEIKRIYPEEPTTIHKVSHEVKLDVNEPLFLTGFKDADVGSDGKALLKKEI
                     TTELLLEIMFGRSSELFERLYEEGLIDDRFSFGYEGHKDYGFCTIGGETKDPEKLHGE
                     LLGCISSTIEKGIDIEDFQRVKKKYTGEFISGFNSLEFIATNFVSYHHKNINIFDYLK
                     VLEEITMQDVTERLNSFFDFSRNATSLVIPK"
     gene            complement(5085..6353)
                     /locus_tag="PK768_03875"
     CDS             complement(5085..6353)
                     /locus_tag="PK768_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQA60137.1"
                     /translation="MDNIFKRKSLKNGINIYIHSTSKFKTITFYLFIHQNLARNTATK
                     TALLPFVLKRGSKSFPTSRKFNLFLEDLYGTTIGGDILKRGEIQIVQFFMEIVNDNYI
                     PGGNILTKALTAFRDMILDPVVEGGAFKKSYVDQEKDVLKRNIESLYNDKFNYVIERC
                     FQEMCKDEAFSIYRYGNVEDLDFIDNENLYSFYRECLSSCPIDFFVLGDVDEDEIARI
                     IEDLFTFERKDTKQVKTNFVTKFIQEPNYAEEKQDVNQGKLTMGFRTNTKYSDEDYYS
                     LMVYNGILGGGSHSKLFQNVREKASLAYYAFSRLEKNKGLMIVSSGIDFDDRERAIEI
                     INQQLEDISEGKISDYEFDSTIKCLVNSFKEAADNPAMIISLYLDGILNGVQRSTKEI
                     IENLYKVTKTDVVNVAERICLDTVFFLNKK"
     gene            6527..6603
                     /locus_tag="PK768_03880"
     tRNA            6527..6603
                     /locus_tag="PK768_03880"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6561..6563,aa:Pro,seq:cgg)
     gene            6721..>7107
                     /locus_tag="PK768_03885"
     CDS             6721..>7107
                     /locus_tag="PK768_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60138.1"
                     /translation="MIQIDDAGSGSLIGGTCVGLYWPKNDVFKYDFVPLECYSPENFK
                     QKKYQEYVVYTIERFFIELGVPKDEEIQLCQGYIFDCLREYLTLKGYRWKSVKIEGLL
                     QEVVEKTFEDYAVSLGTIVQIYLLFYF"
BASE COUNT         1893 a         1644 c         1289 g         2281 t
ORIGIN      
        1 ccgcccccgg catgggcacc tgttcagtca ctacatagcc ttccccctca atccggaccg
       61 acaaaccgtt ctgcattaat atcttccttg catcctcgac atacaggttg cgcaagtcag
      121 ggaccgcaac atgttctctt tcttctacct gctcaatatt gggttctatg cccagatagt
      181 ttagagaatc agccatcact ttctgaaaaa ccggcccagc aattgttcca ccgtatatta
      241 gccctccggt aggttcatct attatgacca aaatggcaag tttggggtca tcaaccggtg
      301 caaaacccat ataagagact acgtattttc caggagaata tttctccgcc gtaccagtct
      361 tgccggcaac tctaaagcct tctaccttgc cccttcctcc ggtcccgttg gtgacgacgc
      421 tttcaagaat ctttttcatt tcctgggcgg tttcatatga cacgacctga cgcaggggag
      481 ctggggaaaa ctcttggacc gtgtttccct ctttatccac tatagctttt gccacatggg
      541 gctggactag tttaccgtcg ttggcaatgg tggaaagtgc cgtaatgagc tgtacaggcg
      601 tcacagatat tccctggcca aaagtaattg tggcaagttc aaccggccca actttcgatg
      661 ggtctaaaat gcctcgggct tcccccggca aatcgattcc ggtcacctcc ccgaaaccga
      721 aacccttaat gtacttcata aacctatcct tgccgagttt cattcccagt tctacaaagc
      781 cgggattaca cgaattttca agcaattgaa taaaggtttg gcttccgtgg cccccatgat
      841 accaacaacc tattctgact cccgaaacca tagcatagcc gggatcgtaa aacttatcct
      901 cgggtcttgc tacaccttcc tccagggcag ccgacgcagt tactatctta aaagtggaac
      961 caggctcgta aacatctgat acagctgcat ttcgccagag tatctgagaa aactcgccgt
     1021 aattattcgg atcataatcg ggtttatttg tcatggccag tatttcaccc gtcttaggat
     1081 ccatcactat aattgttccc cttttggcat tgtgctcggc gagggctttc tcaagttccc
     1141 tttcagcaaa gtgctggata attttatcaa ttgtaagtac taaatctaaa ccatcttcgg
     1201 ggggtatgta tttgtcgact ccaaaaggta gttttctgct caaagcatct ttttcggaaa
     1261 ctatcctgcc gggagtccct ttaagatact tttcatagac caattctata ccatcgagac
     1321 cctgactgtc tataccggta aatcccaaca catgggagca tagattcctt tcgggataaa
     1381 atctcttatg ctcctttgta aaatagatgc cgctaagttt tagattcctt actgtctccg
     1441 attcctcctc agttatcttg cgctttatgt atatggcacc ttttttcttg tttttagcct
     1501 ctcttaatgt ctcctcaagt tcttcccggt ccatatttaa agcggtagac agtaaatttg
     1561 ctgttgtttc tatgtcttta atgtctggcg ggcttgccac tactgtgtcc actgtagcac
     1621 taatggccag aggattttta tttctatcat atacagttcc ccgttttggt tcgacgggca
     1681 catcacttgt ccattggtta agtgcccgct ctttaagttc atctccccga acaaactgta
     1741 tccagaagac acgtcctaat aaaagaaaac tgaaaatcaa tgaaaataaa aggagtataa
     1801 tcaatctcct tttaataagc tgttgaggaa ccgtcattta tttttcaccc acatccactc
     1861 ttatttcgaa tttaaatata tgaattgtct gttgtccggt tctttcatat ttaatttgtt
     1921 tatggcaata tcttcaattc gagctatgga tttgagactg gctatttcca cccttaaacg
     1981 ctcattctgc atactgatat cttcaatttc ggactgcagt ttttcaattc tgtactgata
     2041 ctcggtaatg acggaaaatc tgtacaaaac cgcaaaaagc aaaagggcaa taacagcaag
     2101 ccttaatatg taccgcaatt tgtgatttga atggcttttt tgctgtcgcc tgtgtatttc
     2161 gcgctgatat gcttcttccc tataaaaact tctcctctgt aattcccttt cagctactac
     2221 cactttattc accctttctc ggttttagaa cagaaagctt ttctgcagct ctcaattttg
     2281 cactccgagc ccgagggtta acttcgattt ctccttgtga aggatatatg ggttttgatg
     2341 ttaaaatctt aatggtttta aagtgattac aggtgcagat taaagcattt tttggacaga
     2401 tacaatcgct ggctaaaaac ttaaatgcgt ctttgacaat tctatcttcc agtgagtgaa
     2461 aagttatgac gcaaattcta cctgcgggtt ttaacagttc aactgcatcc ttaatgctcg
     2521 attctaaaat ttcgagttcc ttgttcacgt atatgcgaag ggcctgaaaa gtcttgcggg
     2581 caggatgacc gccctttcgc ctggccctgg caggtatggc atcctttata atgtctacta
     2641 attgtcccgt tgtgattatc ttttgctgct tgcgcctttc gcagatgaaa tcggcaatgc
     2701 gccctgccca gggttcttcg ccatactccc taattatgcg ctttagttcc tctctggaca
     2761 tttcattgac cacgtctttt gcggttaggg gattgcgctt gtccattctc atatcgagga
     2821 ctgcatcctg catgtacgaa aagccccttg accccttatc caattggtga gatgagaccc
     2881 ctaaatcaaa aaggattcca tcgatggcac ttatattgtt ttctctgatg atttttttaa
     2941 cgttagaaaa attatcgtga ataagtctga tgtctccttg atatttagaa aatatttgcc
     3001 ttgcatttga aattgccgct tcgtcctggt ctattccaat aaaagttccg ctatactctg
     3061 ccttctttat tagttcccta aaatgccctc caccacccag tgttgcatca acatacacac
     3121 ccccgggttt gggatttaat atttcaatga cctcgtttaa tagtataggc acgtgatggt
     3181 aattcattag cacaactcct atatgcctaa gtccagggtt tcagcaatat cctcgtaaga
     3241 ctcagcagcc ttgacactgt aggcttccca ttgctcctga ctccatatct ctactctgtt
     3301 ggaaacgcca ataattacaa catctctttc gatgccggca tgttccctaa gctgcggagg
     3361 gatcagtatt ctgccctgtt tatccatttc agcctccact gctcctgcaa aaaagaacct
     3421 tataaaagca cgggcatctt tttgagtgaa gggaagagtt tttaactttt gttccagcac
     3481 agcccactca tctctaggat atacaaaaag acagccgtct aaacctttgg tcaaaacaaa
     3541 gctctggcca aggtcctccc gaaactttga ggggattatc aatctgcctt tttgatcaag
     3601 ggaatgctga aactggccga taaacattat ttttcactcc actttaatgg tgtatcttac
     3661 cacttcactc cacttctccc cacttattct attctatgaa tcttttataa atccttcttt
     3721 tgacaaaaaa aaagatggaa ttaatccatc taaaatacat aggcttttcc tcatttgtat
     3781 atgcgcctaa cgaactattt agggattacc aaggatgtag catttctgga aaaatcaaag
     3841 aaactattca gtcgttctgt gacatcctgc atggttattt cctctaaaac ctttaagtag
     3901 tcgaaaatat taatgttttt gtgatggtaa gacacaaaat tcgtcgcaat aaactcaagg
     3961 gagttgaaac cggatataaa ttcccccgta tacttctttt taactcgctg aaagtcctca
     4021 atgtctatac ctttttctat ggtgctcgaa atacacccaa gaagttcccc gtgcagtttc
     4081 tctgggtcct ttgtctcgcc gcctatcgtg caaaagccgt aatccttatg cccttcatat
     4141 ccgaagctaa acctatcatc gatgagacct tcttcataaa ggcgctcaaa taattccgag
     4201 cttctgccaa acatgatttc caataacaat tcagttgtta tctctttttt caataaggcc
     4261 tttccatcgg aacccacatc agcatcctta aagcccgtta aaaacagggg ctcgttaaca
     4321 tcaagcttta cctcatggct tactttatgg atggtggtcg gttcttcggg atatatcctt
     4381 tttatttcag cctgcggcga taagcctttt gatgcctcat ttttttcaac cagttcgaaa
     4441 acttcctcgg gatctatgca acctatgacg aataaaatca tattgctggg atggtaaaaa
     4501 gtatggtaac acttatacaa ggtttcgaca tcaatatttt gtatggattc caccgtaccc
     4561 ccgatatcaa tcctaacggg gtgtttgtgg tacaagcatt ttaaaaggtt gagcagaagc
     4621 tgccactcgg gctcatcttc atacattctg agttcttgag taattatgcc tttttctttt
     4681 tcaacgcttt cctctgtaaa atatggagac tcaacgaatt ccagcagcaa tttaaggctt
     4741 tctctgaaat gggatgtgcc cgaaaagaga tatgtggtat tggtataact ggtataggca
     4801 ttgggggagg cacccagctc ggagaactta tcaaatacgt ttccgtattc catttcaaac
     4861 attttatgct ctaaaaagtg agctatgcct tccggaactt ttaagtgttc ccccgtaccc
     4921 ggcactatga attcgctgtc tattgacccg taattggtag agtacatggc aaatatttta
     4981 ttataatcct tttttggcag gacatatact ttgaagccgc tttgtgtttc ctttgaaaac
     5041 aagcgttccg ctaaacagct ttccatcatt aaccaccttc cgactcactt tttattaaga
     5101 aagaataccg tgtccaaaca gattctttca gcaacattta ccacatcggt ttttgtcact
     5161 ttatataggt tttcgatgat ctcctttgtg ctcctctgga caccgtttaa aataccgtct
     5221 aaatacaggc tgataatcat ggctggatta tcggcggctt ccttaaagga attgacaaga
     5281 cactttatgg tactgtcaaa ttcataatcc gaaatctttc cctcactaat atcctcgagc
     5341 tgctgattta ttatttcaat ggccctttcc ctatcatcga aatctattcc gctgcttaca
     5401 atcatcaaac ccttgttctt ctccagcctt gaaaaggcat aatatgctaa actggccttt
     5461 tctctgacat tttggaagag cttagaatgg gagccaccgc caaggattcc attgtacacc
     5521 atcagcgaat aataatcctc atcgctgtat ttcgtattcg tcctaaaacc cattgtcagc
     5581 ttgccttgat tcacatcctg cttttcctca gcataattcg gctcttggat gaacttggtc
     5641 acaaagttgg ttttcacctg ttttgtatcc ttgcgttcaa aagtgaagag gtcttcgatt
     5701 attctagcga tctcatcttc atctacgtcg cccagcacaa agaaatctat cggacaggat
     5761 gacaggcatt ccctgtaaaa actatacaga ttctcgttgt cgataaaatc caaatcctcc
     5821 acattgccgt atctgtaaat gctgaaagct tcatctttac acatttcttg gaaacatctt
     5881 tcgatgacat aattaaactt gtcattgtaa agcgattcta tgtttctttt taaaacatcc
     5941 ttttcctggt caacataact ctttttaaaa gctcccccct ccacaactgg gtctaatatc
     6001 atatccctaa aggcagtcaa agcttttgtc aatatgtttc ctccggggat gtagttgtca
     6061 tttactattt ccatgaaaaa ttgaactatt tgtatctcgc cacgctttaa gatatcgcct
     6121 ccaatagtcg ttccgtacag gtcttccagg aacaaattga atttcctcga tgtgggaaaa
     6181 ctcttactgc cccgtttcaa gacaaagggc aaaagtgccg ttttcgttgc ggtattacgt
     6241 gctaaatttt ggtgaataaa taagtaaaaa gtaatggtct taaatttcga tgtagaatgg
     6301 atgtatatgt taataccatt cttcaagctt ttccgtttga agatattatc caaaactccg
     6361 acctcctcaa ttattataga catggacatc ttctcctttt tctacaaaat tcctgctttt
     6421 tttagtttaa ttttatattg tcccccgaaa cactaagtat acagaaaatt acccacacaa
     6481 aaaagggttt gaaataacgg gatgattata gtataataat ttttgtcggg gcgtagcgca
     6541 gcttggtagc gcgttcggtt cgggtccgaa aggccgtggg ttcaaatccc gccgccccga
     6601 ccattaataa atttgcattt tgccttatcg cacacaactt aatactattt gccttaaaac
     6661 cactgtaagg tggttttttt gtttttaaat atgccataat aggcattagg agttgattcg
     6721 atgatacaaa tagatgatgc tggcagcgga agcttaatag gcggaacctg tgttggactt
     6781 tattggccaa aaaacgatgt gtttaaatat gactttgtcc cgctagaatg ttatagtcct
     6841 gaaaacttta aacaaaaaaa gtatcaggaa tatgtagtat acacaataga acgctttttt
     6901 atagagctgg gcgttcctaa agatgaggaa atccaacttt gccagggata catctttgac
     6961 tgtcttcgtg aatatctaac tttaaaggga taccgatgga agagcgttaa aattgaaggc
     7021 ctgctgcagg aagtggtcga aaaaactttt gaagattacg ccgtttcttt gggaacaata
     7081 gtccaaatat atttgctgtt ctatttt
//
LOCUS       DAONUO010000072         7094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_7999_length_7094_cov_4.616849, whole genome shotgun sequence.
ACCESSION   DAONUO010000072 DAONUO010000000
VERSION     DAONUO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7999_length_7094_cov_4.616849"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK768_03890"
     CDS             complement(<1..241)
                     /locus_tag="PK768_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HQA60139.1"
                     /translation="MFIHIGSDQVVLTKDVIMILDKSALGAKATLEFVQIAKEEGFVS
                     DDEIGQNPKDKKSAIVLDKRIVYSPISSVTLLKRAN"
     gene            complement(219..710)
                     /locus_tag="PK768_03895"
     CDS             complement(219..710)
                     /locus_tag="PK768_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQA60140.1"
                     /translation="MENVGEILQNTLSRTRLEKRLNEITIFLKYEDMVGENIAEISRP
                     TFIRNGILFIGVKNHIWMHELYMLKLELMDKINSHLPSPIIKDIKFQICDIKGTKKAK
                     ADIREKKDINYEIPEKIMDFVYNICQDIDDEDLREKFKLLMIKDIKFKMKRGEILVHT
                     HRQ"
     gene            complement(716..1810)
                     /gene="recF"
                     /locus_tag="PK768_03900"
     CDS             complement(716..1810)
                     /gene="recF"
                     /locus_tag="PK768_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HQA60141.1"
                     /translation="MYLANLRLYDFRNFTELDAVFENGINVLYGDNAQGKSNLLESIY
                     FLSTLKPARAFREQELIRHERPLAFVKGIFSTAAGVVDRQVTICRGRKKTVQEGESTK
                     TKWSELSPGISAVYFSPDDVDIVKGQPAGRRRFIDNLIYQARPAFYRYLQGYHRVLSQ
                     RNTLLKTIRSKPNLAKTLDPWDEQLSEFGAQLIGLRLKVLEQLASMAEDFFRDFTGAT
                     DSLKISYKSEIRVGDVDFIKEDFKKKLLEHRKADIQRSYTTVGPHRDDIEFFINGKSV
                     KYYGSQGQQRLVVLCLKFAQRNLLYAERGEYPILLLDDVMSELDSHRRQLLLEEEGHQ
                     VFISTTDKNMLPEKLLNKSALYRVEAGALR"
     gene            complement(1816..2028)
                     /locus_tag="PK768_03905"
     CDS             complement(1816..2028)
                     /locus_tag="PK768_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQA60142.1"
                     /translation="MKEIIINTETIHLDQFLKWAGLVSTGGEAKLIISQGSVKVNGEV
                     ETRRSRKLKISDVVEFEGKSYVVARR"
     gene            complement(2025..3167)
                     /gene="dnaN"
                     /locus_tag="PK768_03910"
     CDS             complement(2025..3167)
                     /gene="dnaN"
                     /locus_tag="PK768_03910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQA60143.1"
                     /translation="MRFLINKDQLIAGISTVEKFVPSKSTVSILSGIKFHIEDDVLHL
                     NANNLEMGIDYKISGETDIIDIQEDGSIVIGSKILSEIVRKMPEGMVEFKTDENIALI
                     SSGDFNMKLPCFDAEDFPEISKIDRQECIVLKQGLFKEMIKQTIFARADETTSRPQLT
                     GILIDYKDNVLNMVALDGYRVAWRYEEQDPEATSFSNDFKVIVPSNTMMEISRIFVDD
                     DEADFELYADKNRVEFKTENILISSAILEGDFIDYARVMDVAPDTVVEVDTTSLQWAI
                     ERANIIAREGNKNNLIIFDIKNNTIEVKADTELGSISDKVLCNTEGEELIIAFNARFF
                     IDALRTIDSSKIKLSFSGSTGPCIITPVEAENHKNLILPVRLGDDM"
     gene            complement(3426..4787)
                     /gene="dnaA"
                     /locus_tag="PK768_03915"
     CDS             complement(3426..4787)
                     /gene="dnaA"
                     /locus_tag="PK768_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQA60144.1"
                     /translation="MTLDPLQIWQHVLKILSNELNNDVSFNTFLKPARLVSLEEGTAI
                     VEVPNDFTQSVIQKRYLNLIKDILSSVLKKNISVLLQINPQGFDAETVPTDAVLLENE
                     PEVKSVLEDIKPSLNLKYTFDTFVVGNSNRFAHAASLAVAQSPAKAYNPFFIYGGVGL
                     GKTHLMHAIGHYILEQDPSCKVLYISSERFTNELINSIRDDKNVEFRNKYRTIDVLLI
                     DDIQFIAGKERTQEEFFHTFNALHDANKQIVISSDRPPKEIPTLEERLRSRFEWGLIT
                     DIQPPDFETRIAILQKKAMMENLVVPSDVINFIATKIETNIRELEGALIRIVAYSSLT
                     DSPIDLSLAEQVLKDILPDEKPKQVTSQQIIEEVGSYFSVSPEEFKSKKRTKDVAFAR
                     QVAMYLCCELTDLSLPKIGEEFGGRDHTTVIHARDKIANDANKDSQLSSLIDNLRKKI
                     LYG"
     gene            5003..5137
                     /gene="rpmH"
                     /locus_tag="PK768_03920"
     CDS             5003..5137
                     /gene="rpmH"
                     /locus_tag="PK768_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQA60145.1"
                     /translation="MKRTYQPKRRYRKRTHGFRKRMLTKSGRNIIRRRRQKGRKRLTA
                     "
     gene            5224..5562
                     /gene="rnpA"
                     /locus_tag="PK768_03925"
     CDS             5224..5562
                     /gene="rnpA"
                     /locus_tag="PK768_03925"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQA60146.1"
                     /translation="MNKSLRLTKNLEFIRVYKTGRRLTSPFFTMYIKKNDLKVTRLGV
                     SVSKKVGKSVVRNKIKRRIKEIIRTNYDHIKQGYDIVVSVRPQAVQLDFKGIVREIKG
                     LMKRGRIYSA"
     gene            5555..5764
                     /gene="yidD"
                     /locus_tag="PK768_03930"
     CDS             5555..5764
                     /gene="yidD"
                     /locus_tag="PK768_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQA60147.1"
                     /translation="MLKKFIILMILFYKKFISPFKPKSCRFYPTCSDYALTAIERYGV
                     VVGGIKALKRIFKCHPFNPGGYDPV"
     gene            5798..6424
                     /locus_tag="PK768_03935"
     CDS             5798..6424
                     /locus_tag="PK768_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HQA60148.1"
                     /translation="MSYLQELMKQLMDWIFVYTNNYGVAIILLTVLIKILLLPFNYMQ
                     INSVKKMQEIAPEQKKLQQKYKNNPDKLNEELMKLYKENNINPMGGCLPLLIQFPFII
                     ALFRVFQTYDFGGAGFLWIKDLSLPDSTYILPILAAATTYWQAKLSTPSGPDNPNATM
                     NIVMPLMIGWFALKFASGLALYWVVSNLVQIAQMLLTRPRALAKDGVK"
     gene            6426..7040
                     /gene="jag"
                     /locus_tag="PK768_03940"
     CDS             6426..7040
                     /gene="jag"
                     /locus_tag="PK768_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HQA60149.1"
                     /translation="MKTLEKQAKTVEEAVQMALEELELRPEDVEVEVLEEGSRGILGF
                     LSKMAKVRVTVKPKPDEVASKFLEGLLEFFDSDAKVECSLNGNVLSIDITGERAGILI
                     GKHGNTLDALQYLTSLVVNRHSKEFIRVLLDAENYRQKREKTLQRLAIKMADKVKENK
                     RSITLEPMYPNERRIIHTALQKDPRVTTKSVGKEPNRRVVISLK"
BASE COUNT         1966 a         1500 c         1494 g         2134 t
ORIGIN      
        1 agtttgctct ctttaaaagg gtgactgatg agataggtga atatactatt ctcttatcaa
       61 gcactatggc actttttttg tctttagggt tctgtccaat ttcatcatcc gatacaaacc
      121 cctcttcttt agctatttgc acaaattcca gagtagcttt tgcaccaagt gcacttttgt
      181 ccaaaatcat gatcacgtct ttagtcagaa ctacctgatc actgcctatg tgtatgaaca
      241 aggatttccc ctcttttcat tttaaatttt atatctttta tcatgagcag cttgaatttt
      301 tcccttaaat cctcatcatc aatatcttgg catatattat atacaaaatc cataattttt
      361 tcaggaattt catagtttat gtcttttttt tctcgaatat ctgcctttgc ctttttcgtg
      421 ccctttatgt cacatatctg aaacttgata tccttgatta ttggactcgg caaatgggaa
      481 tttatcttgt ccatcaattc cagctttaac atgtagagtt cgtgcatcca tatatgattt
      541 ttgacaccga taaagagtat gccgtttcga atgaaggtgg gccttgaaat ttcggcaata
      601 ttttctccca ccatgtcctc gtacttcaaa aagatcgtta tttcattgag ccttttctca
      661 aggcgggtcc tgctcagggt gttttgcaat atttcgccta cattttccac aaacatcacc
      721 tcagtgcacc ggcctctacc cggtacagag cactcttgtt taacagcttt tcaggcaaca
      781 tgtttttatc agttgtcgat ataaagacct ggtggccctc ctcttctaaa agcagctgcc
      841 tgcgatggct gtccagttcg gacatcacat catccaggag taaaatggga tattcccctc
      901 tttccgcata taagagattt cgctgggcaa atttcaggca taataccacc aacctctgct
      961 gtccttggga accgtagtat ttcacggatt tgccattaat aaaaaattct atgtcatccc
     1021 gatggggccc tacagtagtg taactccttt ggatgtcagc tttgcggtgc tctagaagct
     1081 ttttcttaaa atcctctttt ataaaatcca catctccaac acgtatttcg ctcttatagc
     1141 ttatttttaa actatcggtt gccccggtaa aatcccgaaa aaagtcttct gccattgacg
     1201 ccagctgctc cagtaccttt agccgcagac ctattagctg ggctccaaac tccgacaact
     1261 gttcatccca gggatccaag gtctttgcaa ggttcggctt tgaccttatc gtctttagga
     1321 gggtattgcg ctggctaagc acccgatggt agccctgcag gtacctgtaa aatgctggcc
     1381 ttgcctggta tatcaagtta tctataaatc ttcggcggcc cgccggctgg cccttgacaa
     1441 tgtcaacatc atcaggagaa aaataaaccg cacttatgcc aggtgaaagt tccgaccatt
     1501 tggtctttgt gctttctccc tcctgcacgg ttttttttct gccgcggcat attgtcacct
     1561 gccggtcaac cactcctgcc gcagtgctaa atattccctt tacaaaggcc aagggccttt
     1621 cgtgccggat gagttcctgc tccctgaatg ccctggcagg cttaagcgtt gataggaaat
     1681 aaatggattc taataaattg cttttaccct gggcattgtc tccgtataag acgtttattc
     1741 cgttttcaaa cactgcatcc agctctgtaa agtttctaaa atcgtacagt cttaaattgg
     1801 caagatacat acattctacc tccgggcaac gacataactt ttgccctcaa attcaacaac
     1861 atcgctgatt tttaacttcc tggaccttct agtctcaact tcaccgttta cttttacact
     1921 gccctgggaa atgatgagct ttgcttcgcc gccggtggag acgaggcctg cccactttaa
     1981 aaactggtcc aggtggatcg tttcggtatt tatgataatc tctttcacat gtcatcaccc
     2041 agtcttaccg gcagtatgag attcttgtgg ttttcagctt caaccggtgt tattatgcag
     2101 ggcccggtgc tgccggaaaa gctcagcttg atcttagacg aatctatggt cctcaaggcg
     2161 tctataaaaa acctggcgtt aaaggcaatg atcagctcct caccttcagt gttgcacaaa
     2221 accttgtccg aaatgctgcc gagctcggtg tcggctttta cttcaatggt attattcttt
     2281 atgtcaaaaa tgattaaatt gttcttgttg ccttccctgg caatgatgtt tgccctctct
     2341 attgcccact gcaggctcgt agtatcaact tccaccacgg tatcgggtgc tacatccatg
     2401 acccttgcat agtcgatgaa atctccttct aatatcgccg atgaaatcaa tatgttttcc
     2461 gtcttaaact ccaccctgtt tttatccgcg taaagttcaa agtccgcttc atcatcatct
     2521 acaaagatcc gggatatttc catcatggtg tttgaaggca cgataacctt gaaatcatta
     2581 gaaaatgaag tcgcttccgg gtcctgctcc tcgtaccgcc aggccaccct gtagccgtcc
     2641 agggccacca tattcagcac attgtccttg tagtctatca gtatacccgt cagctgtggc
     2701 cttgatgtgg tctcatcggc ccttgcaaaa attgtctgct taatcatctc tttaaagagc
     2761 ccttgtttta agacaatgca ctcctggcga tcgatttttg aaatttcagg aaaatcttca
     2821 gcatcaaagc agggtagctt catgttaaag tcaccggaag atatgagggc gatattttca
     2881 tcagttttga attcaaccat accctcgggc atctttctta cgatttcgga taagatcttc
     2941 gagcctatta cgatgctgcc atcctcctgt atgtcaatta tgtcggtttc gccgcttatc
     3001 ttatagtcta tccccatttc cagattgttt gcgttaagat gcagcacatc atcttctatg
     3061 tgaaatttga tgcccgaaag tattgaaacg gtgctttttg aaggtacgaa tttttctaca
     3121 gttgaaatcc ctgcaataag ctggtccttg ttaatcaaaa atctcatgat atacctccaa
     3181 aaaaaattat tgtttttagg aataaaagtc tactaatccg aatattatat ttttttagta
     3241 gtaatagtag tagtggctgt ggatatgtgg ataaattatg tgaataagta taaattacgt
     3301 cttttgactg tggacaactt gtggaaaatg ggtataactt tattaacagt actaacaaaa
     3361 gttgttaaca gtcgaaaatt gtccactttt cattaacagt ttgtccaaag gatttccaca
     3421 gtttatcaac cgtagaggat ttttttcctt aagttatcaa ttaaggagga caactgggaa
     3481 tctttattgg catcgtttgc gattttatcc ctggcatgta ttactgtggt gtggtcacgg
     3541 ccgccaaatt cttcaccaat ttttggaagg gacaggtctg tgagttcgca gcaaagatac
     3601 attgcaacct gcctggcaaa ggccacatcc ttggtccgct ttttggattt aaattcctcc
     3661 gggctgacgg aaaaataact tcccacttct tctataattt gttgagatgt gacttgtttt
     3721 ggcttttcat cgggcaagat gtcctttaat acctgttcag caagacttaa atcgataggg
     3781 ctgtcggtca gtgacgaata cgcgactatg cgaataaggg caccttcgag ttcccgaata
     3841 tttgtttcga tttttgtggc aataaaattg atcacatcgc tgggtacaac taaattctcc
     3901 atcattgcct ttttctgtaa aatagcaatc cgggtttcaa agtccggagg ctgaatgtcg
     3961 gtgatgaggc cccattcgaa gcgggatctc agtcgctctt ccagggtggg aatttccttg
     4021 ggcggacggt cgctggaaat gactatctgt ttattggcgt catggagcgc attgaaagtg
     4081 tggaaaaact cctcctgggt cctctccttt ccggcgataa actggatatc gtcaatcagc
     4141 aagacatcaa ttgtcctata cttgtttcga aactcgacgt tcttgtcatc ccgaatggag
     4201 ttgatgagtt catttgtaaa cctttctgat gaaatataca aaaccttgca ggaagggtcc
     4261 tgctcgagta tgtaatggcc tatggcatgc atgagatgag tctttcccag acctacacct
     4321 ccgtagataa aaaaagggtt gtatgccttt gccggcgatt gggctacagc caaagatgct
     4381 gcgtgagcga agcgattgct gttcccaact acgaaggtgt caaaggtgta ttttagattt
     4441 aagctaggtt ttatgtcttc caataccgat ttaacttctg gctcattttc aagcagaact
     4501 gcgtcagtcg gaactgtctc agcgtcaaat ccctgtggat ttatttgaag tagtacgcta
     4561 atatttttct taagcaccga actcagtata tctttgatga gatttagata gcgtttttgt
     4621 atgactgact gggtaaaatc atttgggact tccactatgg cagtaccttc ttccaatgag
     4681 accagcctgg caggttttaa aaaggtgttg aatgacacgt cgttatttaa ttcattgctg
     4741 agaatcttta agacatgctg ccagatttgt aaagggtcaa gcgtcataaa aaaacctccc
     4801 cataagcgaa aaaaaaataa aataagccaa tttaataata tcagaataaa agctttcgtt
     4861 caataaagga aaaatattat tccgtgaata tgtttggcgg tgaatttctt gacagtaaat
     4921 tgacttataa gttataatct aaaatagtct agttgggaaa caatacaaat atttttaatt
     4981 tagtggttgg aggtggacag gggtgaaaag gacatatcag ccaaagcgca ggtacagaaa
     5041 gcggactcac ggctttagaa agcgcatgct tacaaagagt ggaagaaata tcatcaggag
     5101 aagaaggcaa aaaggaagaa agagacttac tgcctaataa gggcatcgaa actttaagcg
     5161 accttaacgg tcctaggaag gtcgcttaaa gatatatata ctctttcccc tggaggatat
     5221 ctattgaaca agtctttaag attaacaaaa aatttagaat tcattcgagt atacaaaacg
     5281 ggaaggcgtt tgaccagccc tttttttact atgtacatca aaaagaatga tctaaaggtc
     5341 acccgcttag gggtttccgt ctcgaagaaa gtaggcaaaa gcgtcgtgag gaacaagata
     5401 aagcggcgca ttaaggaaat tatcaggact aattacgatc atataaaaca agggtacgac
     5461 attgtagtct ccgtgaggcc gcaggctgtc cagcttgatt ttaaggggat agtccgtgaa
     5521 atcaaaggcc tcatgaaaag agggcgcata tatagtgctt aaaaagttta taattttaat
     5581 gatactcttt tacaaaaaat ttatttcccc ttttaaaccg aagagctgca ggttttatcc
     5641 tacgtgctcc gattatgccc tgacggcgat tgaaaggtac ggggtggtag taggtggaat
     5701 taaggcctta aaacgcattt ttaagtgcca tccctttaat cccggaggat acgaccccgt
     5761 ttaaatacat ttatgataaa attaaggggg atcgaccatg tcctatcttc aagaactcat
     5821 gaagcagttg atggattgga tttttgtgta taccaacaat tacggtgtag caataatttt
     5881 actgacggtg ctaataaaga ttcttttact gccctttaat tacatgcaga ttaattcggt
     5941 aaagaagatg caggaaatag cacccgagca gaaaaagctc cagcaaaagt acaagaacaa
     6001 tccggataag ctaaatgagg agttaatgaa actgtacaag gagaacaata tcaatcccat
     6061 gggaggatgc cttccgctgc tgattcagtt cccttttatc attgcccttt ttagagtgtt
     6121 tcagacttac gatttcggtg gtgccggatt tttgtggata aaggatctga gcttgccgga
     6181 tagtacgtac atattgccaa tattggctgc agcaacgacc tattggcagg caaaattatc
     6241 tactccttcc ggaccggaca atccaaatgc caccatgaat atcgtgatgc ctctcatgat
     6301 aggctggttt gccttgaaat ttgcatcggg tctggcattg tactgggttg tgagtaactt
     6361 ggtgcaaatt gcacagatgt tgttgacacg accacgagcg cttgccaagg atggtgttaa
     6421 atgacatgaa gactttggaa aaacaggcca agaccgtaga agaagcggtc cagatggcct
     6481 tggaggaact cgagctaagg cctgaggatg tggaagttga ggtattggaa gaaggcagta
     6541 ggggaatact aggttttttg tccaaaatgg caaaggtaag ggtaacggta aagccgaagc
     6601 ccgatgaagt tgcgagcaag tttttggagg gtttactgga gttttttgac agcgatgcca
     6661 aagtcgaatg ctcactaaat ggcaatgttt tgagcatcga tatcacgggc gaaagggcag
     6721 gaatacttat aggcaaacac ggaaacactt tagatgccct tcaatattta acttccctgg
     6781 tggtcaaccg acattctaag gaattcataa gggttctgct ggatgcggaa aattacaggc
     6841 aaaagcggga aaagaccctg cagcgactgg ctattaagat ggccgacaag gtgaaggaaa
     6901 acaagcgaag cataaccctt gaacccatgt atcccaatga gcgccgcatt atacatacgg
     6961 ctttgcaaaa agatcccagg gttacaacaa aaagtgtagg caaggaaccc aacagaaggg
     7021 ttgtcatatc tctaaagtaa gtacattaag tggccccatt gcgtaaagct tggggctttt
     7081 taaaagcggt gata
//
LOCUS       DAONUO010000073         7074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8039_length_7074_cov_4.296623, whole genome shotgun sequence.
ACCESSION   DAONUO010000073 DAONUO010000000
VERSION     DAONUO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8039_length_7074_cov_4.296623"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PK768_03945"
     CDS             <1..426
                     /locus_tag="PK768_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA60150.1"
                     /translation="KLPPLRERKEDISIYVKYLMDKIKHRLDKEIHNITPKALRTLMN
                     YHFPGNIRELENILEKSIVMDEDGIIDVDDLPEFLNLESRQNGIYIDFEHDWPALSDV
                     ERTMIEKTLVAFPNKTKAAQVLGISRATLYRKMKELGLD"
     gene            707..2089
                     /locus_tag="PK768_03950"
     CDS             707..2089
                     /locus_tag="PK768_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQA60151.1"
                     /translation="MFKRVPEPFKIKMVEPLKIKSREERIKALEAAGYNPFLLDSDDV
                     YIDLLTDSGTGAMSDYQWSGLMLGDESYAGSKNYRNLKETVEDLFGYSYALPVHQGRG
                     AEKVLFPCLIKEKGQFVLGNMHFDTTKAHIELNGARAVNLVTEKAFDTATPYDWKGNF
                     DLDKLESFIKEHKPENVAFILITVTCNSAGGQPVSMANIRAVSEIAKKYNIRVFFDAA
                     RFAENAYFIKQREKEYKDKSIREIVKEMFSYADGFTMSAKKDAIVNIGGLIGIKDDED
                     LYNDASSRIVPYEGFITYGGLAGRDMECLARGLEEGIDEDYLHYRISQVAYLGHRLIE
                     GGIPIQTPTGGHAVFVDAKKLLPHIPYYQFPAQVLVNALYIESGVRAVEIGSFMLGRD
                     PETGEQLESPLELMRLTIPRRVYTNDHMDVVADALIAIKDKAKDLKGLEFTYEPKVLR
                     HFTARLRPIE"
     gene            2147..3502
                     /locus_tag="PK768_03955"
     CDS             2147..3502
                     /locus_tag="PK768_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HQA60152.1"
                     /translation="MGSKKDAKVSRGQWATSIGFVLAAAGAAVGLGNVWKFPYLTALH
                     GGGTFLFAYILMLLFLGIPVLVTEMVLGRRGKLDPVGTYSKLSGGSGFWKFVGYVAVS
                     VNFIVLSFYSVVGGWITNYMFQYIMGGIKGDIVEYFGGFVSNPYSPLLWYALFMGMTI
                     YIVAQGVSAGIERASNIMMPTLFLLFIILAIRAVTLPGAIEGIKYYLLPDFSKLSGTT
                     FLMAMGQVFFSLNIGAGCTMTYASYLSDDENIPKMSLQVPLLDFLAAFLAGIIIIPSV
                     FAFNLDPAAGPPLLFITMPFVFGNMPLGVLFGLMFFVLMLFAALSSSISMLEVNTSFL
                     VDNYKIDRKKAALTAGFFIFLLGIPSSLAQGVLSNFTIFGLDFLSAMDFLASYILMPF
                     GAFMMTIFLVRVLGLDEAINEATNEGTIPFKIKVLWSFLVKYVVPIIIILVFLSSVGL
                     I"
     gene            complement(3617..4672)
                     /gene="cobT"
                     /locus_tag="PK768_03960"
     CDS             complement(3617..4672)
                     /gene="cobT"
                     /locus_tag="PK768_03960"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HQA60153.1"
                     /translation="MNKLELTLQKIKPLDVDVMKRTQKRLDELTKPPGSLGVLEDIAK
                     QIAGITGNVIPELPRKAAILMAGDHGIVKEGVTPYPQEVTAQMVLNFVNGGAAMSVLT
                     RHENADLYVVDIGVATDLPDLPNIIKRKVAYGTKNMAEGPAMTEAEAIRALEVGIEIT
                     EEVINGGVGLIAIGEMGIGNTSPSTAIISTYSGLSVKEVVGRGTGVDDERLKTKIEAI
                     EKALEVNKPDPAKPIDVLSKVGGLEIAGMAGVILACAANRVPVIIDGFISGAAAVIAS
                     EIAPLAKNYMLGSHLSEEPGHRVMLDYLKIKPMLIMNMRLGEGTGAALAMNLVDASLK
                     TLKEMSTFAEAGISSKD"
     regulatory      complement(4855..5036)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5299..5889
                     /locus_tag="PK768_03965"
     CDS             5299..5889
                     /locus_tag="PK768_03965"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQA60154.1"
                     /translation="MKIVQYFVPPTKYNVKCPYFMDAEFIVVHNTANDASALNEVRYM
                     ISNSNKVSFHYAVDDREIVQGIPDDRNAWHAGDGENGLGNRKGLSIEICYSKSGGNRF
                     KEAEKLAVQFIAYKLKEKGWGIDKVKKHQDFSGKYCPHRTLDMGWQRFLYMVKSELDN
                     LNEKKEGNIDKPSSWASREVKHMLRGLRYISLKTAS"
     gene            complement(5929..6918)
                     /locus_tag="PK768_03970"
     CDS             complement(5929..6918)
                     /locus_tag="PK768_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HQA60155.1"
                     /translation="MKSNVIITEEMRFRQRVVEYAIKHDNNAKAARRYHTSRQQVQRW
                     RKRYDGTVQSLANRSRRPHSHPNQHTKQELDLIKRKYRYHGHEGLAQVYRKLKDAGYT
                     RSYDSMCRQIRKMKLNKPVKKQNPPKTKQKKNKAKYPGERVQIDIKYVPNECIGFASN
                     TSRYYQITAIDEYSRKRYMEIVNEKSTYTTSNFLLELEKKLGFKVELVQTDNGLEFVN
                     DLEVTQKKTRFEIFLEKLGIKHRRIRPYSPWQNGIVERSHRIDNELFYSKRRFRSYEE
                     MQKAFRRYNNRYNNIARKVLGFKTPNEIVESNMKNLKNSVGNCTVSLMNVKMR"
BASE COUNT         2054 a         1331 c         1458 g         2231 t
ORIGIN      
        1 aagcttccgc ctttaaggga acgaaaagag gatatttcca tttacgtgaa atatctcatg
       61 gacaagatca agcatcgtct ggataaagaa attcacaaca tcacaccgaa ggccttgcgt
      121 accttgatga attaccactt tccaggaaat attagggaac tggaaaacat actggaaaaa
      181 agtatagtaa tggatgaaga cggaattata gatgtggacg acttgccgga atttctcaac
      241 cttgaatcaa gacaaaacgg catatatata gattttgaac atgattggcc ggcattaagt
      301 gacgtagaaa gaaccatgat agaaaaaacc ctagtagctt ttcctaataa gacaaaggct
      361 gcacaagtcc tcggcatatc cagggcgact ttatatagaa aaatgaaaga attaggattg
      421 gattaacagc gtatcaaatt gagacactcg tatttaaata atttaccaaa aataaagaag
      481 tttcaaatgc atgattcaat atgagacaat aacaagtaat atcgtctcat attaacacaa
      541 tatcaactgt aattctaaac gaatatattt atgagtcatc aattagaact cctcccgagt
      601 catgattcta cgcacaccgg ggcctaataa cttctttgca ttcagctttg gcatgaattt
      661 tgcaaagaga tgttattgaa caaaaaaaga ggggaggagt tttagtatgt ttaaacgagt
      721 gccggaacca ttcaaaataa agatggtaga acctttaaag attaaaagca gagaggaaag
      781 gataaaagcg ctggaagctg cagggtacaa tccattttta ttggattcag atgatgtgta
      841 tatagacctc ctcactgata gtggtacagg tgccatgagt gattatcagt ggtcgggttt
      901 aatgttgggg gatgaatcct acgcaggcag caaaaactac aggaatctaa aagagactgt
      961 tgaggacttg tttggttatt cgtacgctct tcctgtgcat cagggaagag gcgctgaaaa
     1021 agtattattc ccgtgcttaa taaaagaaaa gggtcaattc gttttgggga atatgcattt
     1081 tgacaccact aaggcccata ttgagttaaa tggcgcacga gctgtaaatc tagttactga
     1141 aaaagctttt gatactgcta ctccttatga ttggaagggg aactttgacc ttgacaagct
     1201 agaaagcttc ataaaggaac ataaaccgga aaatgtggca tttatattga ttaccgtaac
     1261 ctgcaatagc gccggtggcc agccggtctc tatggccaac ataagggccg taagcgaaat
     1321 agccaaaaag tataatatta gagtattttt cgatgccgca agatttgctg aaaacgccta
     1381 ctttataaaa cagcgagaaa aggagtataa ggataaaagt atcagagaaa ttgtaaaaga
     1441 aatgttctct tacgcggatg gctttaccat gagtgccaaa aaagatgcta tagtaaatat
     1501 tggcggcctt ataggaataa aggatgatga agatttatac aatgacgcat ccagtagaat
     1561 cgtaccttac gaaggcttta taacttacgg tggtcttgca ggaagggata tggaatgtct
     1621 tgcacggggt cttgaagaag gcattgatga agactacctg cattatcgca tatcacaggt
     1681 tgcctactta ggtcaccgat tgattgaagg aggaatcccc attcagaccc cgacgggtgg
     1741 ccatgcggtg tttgtggatg ccaaaaaatt actcccacac ataccgtact atcaatttcc
     1801 tgctcaggta ttggtaaatg ccttgtatat cgaatcgggt gtaagggctg tggaaatagg
     1861 ttccttcatg ctgggaagag atcccgagac cggagagcag ttagaatctc ctttagagct
     1921 gatgagactt acaattccgc gaagggtata tactaacgat cacatggatg tagttgcgga
     1981 tgcgctgata gctatcaagg acaaagccaa ggatttaaaa ggcttggagt ttacctatga
     2041 acctaaagtt ttgaggcact ttactgcaag gttaagacct atagaataat aaaatattgg
     2101 ccaccacagg cttcctttgt ggtggcctga ggtaaaggag gatactatgg gaagcaaaaa
     2161 agatgcgaag gtcagccgcg gccaatgggc tactagcatc ggttttgttt tagcagcagc
     2221 tggtgctgct gtaggattgg gcaatgtatg gaagtttccc tatcttacag ccttacacgg
     2281 cggaggtacc tttctcttcg cctatatttt aatgctgctg tttttaggga taccggtcct
     2341 ggtaacggaa atggttttgg ggagaagagg gaagctagat ccggtgggga cgtattcaaa
     2401 actgtccggc ggaagtggct tttggaaatt cgtaggttat gtagctgttt cggtaaactt
     2461 tatagttttg tccttttact ctgtagtagg tggatggata acaaattaca tgtttcagta
     2521 tataatgggt ggcataaaag gcgatattgt tgaatatttt ggtggttttg tatcaaatcc
     2581 ttatagtccc cttttatggt atgccttatt tatgggaatg acaatttata tcgtagcaca
     2641 aggtgtatct gctggtatag aaagagcgag taatataatg atgcctacct tgttcttgct
     2701 tttcatcatc cttgctataa gggctgtaac cctccccggt gcaatagaag gcattaagta
     2761 ttacctgctg ccagatttca gtaagctttc aggaactact tttctaatgg ccatgggcca
     2821 ggtgttcttt agtttaaaca ttggcgcagg ctgcaccatg acttatgcca gttatctttc
     2881 cgatgatgaa aacataccaa agatgtccct tcaggtgccc ttattggact ttttagctgc
     2941 ctttttagct ggaataatta ttataccttc ggtctttgca ttcaatctgg atcctgcagc
     3001 aggtccgccg ctgctgttta ttacaatgcc atttgtgttt ggaaacatgc ctttaggagt
     3061 tctttttggc ctaatgttct ttgtgctgat gttatttgca gctttaagtt catctatttc
     3121 aatgttggaa gtcaacacgt ctttcttagt cgataactat aaaatagata gaaaaaaagc
     3181 agctttgacg gcaggattct tcatcttttt gttgggaata ccatcttcgc ttgctcaagg
     3241 agttttgtct aatttcacca tattcggctt agactttcta agtgcaatgg actttctagc
     3301 atcctatata ctaatgccct ttggagcctt tatgatgaca atattcctag tcagggtatt
     3361 aggtcttgat gaggcaataa atgaagctac aaatgaaggc acaataccct ttaagataaa
     3421 agtgctgtgg agctttttag taaaatatgt agtacctata ataatcatat tggtgttctt
     3481 aagttcagtt ggtcttatct aataaaagct gaattaatgg aaaagagacc tacaaacaat
     3541 ccctccgaag aatttcgcag agggattgtt tgttgtttta ggcaaaagaa acagtagaat
     3601 cgctttatga taatgtttaa tctttagagg atataccggc ttcagcgaat gttgacatct
     3661 cctttaatgt tttcaaactg gcatctacca ggttcatggc tagagccgca ccggtgcctt
     3721 cacctaaacg catattcata attagcattg gcttgatttt taaataatcc agcattactc
     3781 tatggccggg ttcttcagaa agatgggagc ctaacatgta gtttttggca aggggcgcta
     3841 tttcggacgc tattactgca gctgctccgg aaataaaacc atcgatgatg accggtactc
     3901 gatttgcagc acaggcaagt atgaccccag ccatgccggc tatttcaagg ccacccacct
     3961 tagataacac atcaatgggt tttgcaggat caggcttatt cacctctaga gctttttcaa
     4021 tagcttctat ctttgttttc agtctttcat catcaactcc ggtgccgcga cctactactt
     4081 ctttaacaga aagaccgctg taggttgaaa ttattgctgt gctgggcgag gtattcccaa
     4141 ttcccatttc accgatggct atcagtccta caccgccatt gatgacttct tctgttattt
     4201 caattcctac ttctaaggcc cttatagcct cagcttcagt catagccggt ccctcagcca
     4261 tattttttgt gccgtatgct acctttcttt tgataatgtt tggcagatca ggtaaatctg
     4321 tggcaactcc aatatctacc acatataggt cagcattttc gtgtcttgtc aaaacactca
     4381 tggccgcacc gccatttaca aaattcagca ccatttgagc agtcacttct tgaggatatg
     4441 gagtcacacc ctctttgacg ataccatgat ctcctgccat taaaatcgct gcttttctcg
     4501 gcagttccgg aatcacgtta cctgttatgc ccgcaatctg ttttgcaata tcttccagca
     4561 ctcctaaact acccgggggc tttgtcagtt catccagccg cttttgagtt ctcttcataa
     4621 catccacatc tagaggttta atcttttgta aagttagctc aagcttgttc acaacatctc
     4681 ctccttaagt ttattattat aatgaaacat caaagacgtt tgtaaacatg gatattgttc
     4741 ctagcttaaa agagctaaaa tctcagaccc tgtaaacaac gatatgtgat tagcacgtta
     4801 taaaaaaagt cctaagattg aatgactttc aatcttagga ctttaccatc atcctaatag
     4861 atctccttat taggcaggtc tcctggcttg cagtcatcgc tcctccacgc cttcccaaag
     4921 gctaaaagct ccagtggcaa atgtggacag ctcctactca cagtggcggg tccgctcagg
     4981 ccttgcacct gattccctat tctcccaaaa tgggcaccta ataagtttat agtattcagt
     5041 ttactattat atatatttat attcaccata aaagaacgtt ttccttctac tgttataata
     5101 ttttattaat aagaacggta aacaactaaa attgaaggtc aatcaaaggc caagctgcat
     5161 atcatatatt aaattgataa agttcatgaa atgtaatgct attgaatgcg caaaatatga
     5221 agatgctttg ccttgtttta aatattttaa atgcagtttt atttaatcta ttcaaagctt
     5281 gtcaaaggag gcgaggttat gaaaattgtt cagtatttcg tgccgccaac taagtataat
     5341 gttaaatgcc cttacttcat ggatgcggag tttatagtag tgcataacac cgcaaatgat
     5401 gcaagtgcgc ttaatgaagt aagatatatg ataagtaata gtaacaaggt ttcctttcac
     5461 tatgcagtag atgacagaga aattgtccag ggcataccag atgaccgcaa cgcttggcat
     5521 gcaggcgatg gagaaaacgg attgggaaat agaaaagggt taagtattga aatatgttat
     5581 tccaaatcgg gcggaaatag atttaaagag gccgaaaaat tagctgttca gtttatagca
     5641 tacaaactaa aggagaaagg ttggggaata gataaggtaa aaaagcatca ggatttttcg
     5701 ggaaaatact gtccccacag gactcttgat atgggttggc aaaggttttt atacatggtg
     5761 aaatctgaat tggataattt aaatgagaaa aaggaaggga atattgacaa accttcatct
     5821 tgggcatcta gagaagtcaa acatatgtta cgcggcctca gatatatatc cttaaaaact
     5881 gctagttaaa tcttactgtg taaggaagga atgtcaagat caaaagcgtt agcgcatctt
     5941 gacattcatg agtgaaacag tacaattgcc aacgctgttt tttaggtttt tcatgttact
     6001 ctcaactatt tcattcggtg tcttaaaacc gagaactttc cttgcgatgt tgttgtatcg
     6061 attattgtat cgcctaaatg ctttctgcat ttcttcatag cttctaaacc gccttttgct
     6121 ataaaatagc tcgttatcta ttctgtgact acgctcaact atgccgtttt gccatggaga
     6181 gtatggcctt attctgcgat gttttatacc caatttttct agaaatatct caaatctcgt
     6241 cttcttttgg gttacttcta aatcattgac aaactctaaa ccgttatcag tctggactag
     6301 ttccaccttg aatcctagtt tcttttccag ttccaataag aagttggatg tggtataggt
     6361 gctcttttca ttaactattt ccatgtatct ttttctcgaa tattcatcaa tcgctgttat
     6421 ctggtaatat ctactggtat tactggcaaa gcctatacat tcattaggaa catattttat
     6481 gtctatttgt acacgttccc cagggtattt tgctttattt ttcttttgct ttgttttagg
     6541 ggggttttgt tttttaactg gtttgtttag tttcatttta cggatttgac ggcacatgga
     6601 gtcatagctc ctagtgtagc ctgcatcttt taatttgcgg tatacttgag ctaatccttc
     6661 atgaccatga tatctgtatt tgcgcttgat tagatctaat tcttgctttg tatgctgatt
     6721 tggatggcta tggggccttc tgctacgatt agctaaggat tgtaccgttc cgtcgtatct
     6781 tttacgccat ctttgtacct gttgtctgct ggtatggtat ctcctggcag ctttagcatt
     6841 gttgtcgtgc tttatagcat attctactac cctttgacga aaacgcatct cttcggttat
     6901 aattacatta gatttcataa gaaaggtcct ccgattattg ttttttgacc atattaaaca
     6961 ttatatcaga ggcctttctt atttttttgt tttttgtcac aaatgtatta tctcatttca
     7021 gtttgatgca agaatattat tccgcatttg aataaatttg cggatatagg ggaa
//
LOCUS       DAONUO010000074         7062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8051_length_7062_cov_4.259027, whole genome shotgun sequence.
ACCESSION   DAONUO010000074 DAONUO010000000
VERSION     DAONUO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8051_length_7062_cov_4.259027"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /gene="murD"
                     /locus_tag="PK768_03975"
     CDS             <1..994
                     /gene="murD"
                     /locus_tag="PK768_03975"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQA60156.1"
                     /translation="TTLIGEIFKNEGKNITLAGNIGIPLVREVEKAEAKDFLIVEVSS
                     FQLENIMHFKPKISVILNISEDHLNRHKTFENYIETKARIFENQTEEDYAVLNYDDPI
                     VRSFIKRIKAKTMFFSQKEELSRGIYVKNGVIVIRENGKIYPLLKAEELGIKGSHNLE
                     NALAAACVAWICRINLNNVAETLKDFHGVEHRLEFVAEIGQVKFINDSKATNPHSAQK
                     AIEALNEPIVLIAGGYDKKSDYTDFIRAFSGKVKKLILIGDTADVIEDAAKKQGFYDI
                     QKTDSLQQAVKLAYSAATPGDVVLLSPACASWDMFESFEERGRIFKEAVYSLKT"
     gene            1008..2105
                     /gene="spoVE"
                     /locus_tag="PK768_03980"
     CDS             1008..2105
                     /gene="spoVE"
                     /locus_tag="PK768_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HQA60157.1"
                     /translation="MSRRPPDFAILFTVLILVCFGMIMVFSSSSVRAYYFYNDSFYFF
                     KRQLLWSLLGFVAMVFFMNYDYWKIKKHEKLIVFIMFLLLVLVLIPGIGKKVNEARRW
                     IGIGALTLQPSEVAKLGMIIYLSCGLERKGDKVQSFFKGLLPFLLVMGAVCALILKEP
                     HLSATVLIGMTTLVMLFVGGAKMAHMIGLGAAGSALAVMLALSKPYRLKRILSFIDPW
                     KNPSEEGYHIIQSLYALGSGGLIGVGIGQSRQKFFYLPEPQTDFIFSVIGEELGFIGA
                     AFVIIAFMFFIWRGYRTAMAAPDMFGKLLATGITTLIALQFLINVAVVTASVPVTGMP
                     LPFISYGGSSLTITMCQIGILLNISRYAEVA"
     gene            2107..3222
                     /gene="murG"
                     /locus_tag="PK768_03985"
     CDS             2107..3222
                     /gene="murG"
                     /locus_tag="PK768_03985"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQA60158.1"
                     /translation="MPAKKVILAGGGTGGHIYPAIAIAQAIRKSFPDSDILFIGTERG
                     LEKELVIKAGFPLKTIRVRGFRRKLSIDTLISVKELVHGAMDSIGLLKREKPDIVIGT
                     GGYVAGPAVFFASILGIPTAIHEQNVTPGVTNKILSRFVDKIFISFPDSMKYFPKEKT
                     LLSGNPVRKEITKGNRLMALKKFGLSPDLPVVLCFGGSQGAARINDAVCHIIEAIKDA
                     ASFQLIHITGKNHYERIRNLLAKKGIDTEELGNIILRPYIHEMQEAYAAADLVISRAG
                     ALSIAELSVCGKPAILIPLSTAANQHQDFNAKFMERNGAAVIIDEDKLSGKSLLDTIE
                     SIIFDKHCLHKMSMASKNLAIEDAAEKILAEIIKLVK"
     gene            3360..4769
                     /gene="murC"
                     /locus_tag="PK768_03990"
     CDS             3360..4769
                     /gene="murC"
                     /locus_tag="PK768_03990"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQA60159.1"
                     /translation="MTGNYNRIHFIGIGGTGMSGIAKILMNMGYNVSGSDLKVSEALI
                     RLRDAGANVFIGHHVSNVIGADLVVVSSAIPKTNPEYVKAIESNIPVVHRADMLSLLM
                     DSKKGVAVSGAHGKTTTTSMISLIMEKNGFDPTVIIGGELNDIGGNATLGSGEFLVAE
                     ADESDGSFVKLKPYIAVITNIENDHMDYYKNMENMKRAFKEFAGNVIEDGFIVLGNDN
                     EHVRELEKDIDKEFYTFGINHNSDFMPKNIRLEGLSTRFEIYFRGKLLGEMQLNVPGI
                     HNIVNATAAIAVGNRLGISIEGMREAIKTFRGVQRRFQIVGDIDGIKVIDDYAHHPTE
                     IKATLRAAKNCRPRKVYAIFQPHRYTRTQNLSKEFGSSFFDADEIVLTDIYSAGETPI
                     KGVSSDLIAEYLRENGKKVVYIKRKEEIPDYIIDKKLVPGDYILTIGAGDISDVAYSI
                     VSKLKSRQKGVIGNIPEVK"
     gene            4776..6032
                     /gene="murA"
                     /locus_tag="PK768_03995"
     CDS             4776..6032
                     /gene="murA"
                     /locus_tag="PK768_03995"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQA60160.1"
                     /translation="MYIIQGGETLSGKIKVQGSKNSSLPILAATVINGKKNIICDVPL
                     IKDIEVMCKILSNLGATIVNRGNVIEVDSEKVNSFEVPEQLMREMRSSIILMGALLGR
                     FGRAKVSYPGGCEIGPRPIDLHLKGLSLMGVDFSEGHGFLNAKTDGLKGADIHLDFPS
                     VGATENLMLAGVLAHGTTIVRNAAKEPEIVDLQNFLNRMGANIKGAGTDTIKIEGVKI
                     HDLRAVNDYTVIPDRIVAGTYLVAAAITRGHIILTNVITEHLEPILAKLRETGCVINS
                     RNGQVELYVDKPMVSLESLRTLPYPGFPTDMQAPMMALLATVEGTSILTETIFENRFK
                     HAQELRRMGANITINGNTAIIKGVKKLTGAIVEAKDLRAGAALIVAAFAAEGETVVEG
                     ACHVERGYEDIVGKLKALGAKIVKKH"
     gene            6166..6981
                     /locus_tag="PK768_04000"
     CDS             6166..6981
                     /locus_tag="PK768_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQA60161.1"
                     /translation="MGRFGENRNYKIATRRKRIINYKRLLLSLAFLCIISLMPTLYSS
                     LFAIKDVQIRGNKTISSEEILSVIDNYYDTNMLKLSAQQVKQDLQGTIPIKDVRVHYK
                     LPHTLILEVEEREVAAALHYLNSFALIDSQGIIVKSVTRLENYSIPIVTGLKVVDAKI
                     AKKPVFEEYPMHYETLLELIDAAKPILHELSEINLVVDANKDTSFYLYTLDGYQVFLG
                     QWDDKKITTMRDLLADIRDKNLGKGLLDISHNTPIFKPFSNSDKSGEEAKDEE"
BASE COUNT         2242 a         1223 c         1612 g         1985 t
ORIGIN      
        1 aacaacttta atcggcgaaa tctttaaaaa tgagggcaaa aatattactt tggcaggcaa
       61 cataggcatt ccgttagtca gggaagttga aaaagccgag gcaaaggact tcttgatagt
      121 ggaagttagc agttttcagt tggaaaatat catgcatttt aagcctaaaa taagcgtaat
      181 attaaatatc agcgaagacc acttaaaccg tcacaagact tttgaaaatt atattgaaac
      241 caaggctagg atttttgaaa accaaactga agaagactat gcggtgttga actatgatga
      301 cccaattgtc aggtctttta ttaagaggat aaaggccaag actatgttct ttagccaaaa
      361 ggaagagctt tcccgcggaa tttatgtaaa aaacggtgta atagtaataa gggaaaacgg
      421 caagatatat cccctgctta aggctgaaga gctggggata aaaggaagcc acaaccttga
      481 gaatgcctta gcagcagctt gtgtggcgtg gatttgccgg atcaatttga acaatgtagc
      541 cgagactcta aaggactttc atggcgtaga gcatagactg gagtttgttg ccgaaattgg
      601 tcaagtcaaa tttattaacg actcaaaagc tacaaatcca cattcggctc aaaaggccat
      661 agaagcttta aatgaaccca tcgtcctcat tgccggtggt tacgataaaa aaagtgatta
      721 cacggatttc atcagggctt ttagcggaaa ggttaaaaag ctgattctca taggggatac
      781 tgccgacgtt atagaggatg ctgccaaaaa acagggtttt tatgacattc aaaaaactga
      841 ttccctgcag caggccgtaa aacttgcata tagtgccgcg acgcccggcg atgtggtgct
      901 cttatctcct gcttgtgcca gttgggatat gtttgagagc tttgaagaaa ggggacgaat
      961 ttttaaagag gcagtatatt ctttgaaaac ctgaaggagt gatatatgtg tcccggaggc
     1021 caccggattt tgccatactc tttacggtcc tcatactggt atgtttcggt atgataatgg
     1081 tgtttagttc tagcagcgtg agggcgtatt atttttataa cgatagtttt tattttttta
     1141 aaagacagtt gctctggtcc ctcttgggct ttgtagcaat ggtatttttt atgaattatg
     1201 actactggaa gataaaaaaa catgaaaaac taatcgtttt tatcatgttt ttgctgctgg
     1261 tgctggtgct gattcccgga atcggtaaga aagtcaacga agctcgccgc tggataggca
     1321 taggagcttt gacattacag ccctctgaag ttgcaaagct agggatgatc atatatcttt
     1381 cctgtggatt ggaacgaaaa ggggacaagg tccaatcttt tttcaaggga ttgttgccat
     1441 ttttattggt aatgggagct gtttgcgcct tgattttgaa ggagccgcac ctgagtgcca
     1501 cggtgctcat cggaatgact acacttgtaa tgctgttcgt aggtggtgct aaaatggccc
     1561 atatgatcgg cttgggggct gcaggctctg ccttggcagt tatgctggca ttgagcaaac
     1621 cttatagact aaagcgcata ctatctttta tagacccatg gaaaaaccca agtgaagagg
     1681 gataccatat aatccaatca ctatacgcct tgggttcagg cggattgata ggcgtaggca
     1741 tcggtcagag taggcaaaaa tttttttatc ttccagagcc tcaaaccgat tttatttttt
     1801 cggttatcgg agaggaactt gggtttatag gtgcagcctt tgtaataata gcctttatgt
     1861 tttttatctg gaggggctac aggacggcga tggctgcacc ggatatgttc ggaaagcttt
     1921 tggcaaccgg cataaccact cttatagctt tacaattttt gataaatgta gccgtagtaa
     1981 ccgcatcggt accggtgacg ggtatgccgc ttcccttcat aagctacggt ggctcttccc
     2041 ttacgataac catgtgtcaa attggaattt tattaaatat ttccagatac gcggaggttg
     2101 cttaaaatgc cagcaaaaaa ggttattctt gctggtggcg ggaccggcgg tcatatttat
     2161 ccagctatag caattgcaca ggcaatccgt aaatcgtttc ccgattcaga tatcctcttt
     2221 ataggaacgg aaagaggtct tgaaaaagaa ttagtcataa aggcggggtt tcccttaaaa
     2281 accataaggg tgagggggtt tcggcgcaaa ctatcaatag acaccctgat ttctgttaaa
     2341 gaattagtcc atggagccat ggattcaata ggtttattaa aaagggaaaa acccgatatt
     2401 gtcataggga ccgggggtta tgtagcaggc cctgccgttt tttttgcttc tatcttgggg
     2461 atccctactg ccatccacga acaaaatgtt actcccggcg ttacaaacaa aatactgtca
     2521 cgcttcgtcg ataaaatttt tataagtttt cccgattcta tgaaatactt tcccaaagaa
     2581 aaaactctcc tttctggcaa tcccgttaga aaagaaatca caaaagggaa cagacttatg
     2641 gctttaaaaa aattcggcct atctccggat ttgcccgtgg ttttatgttt tggaggcagt
     2701 cagggtgctg cacgcataaa tgatgctgtt tgtcatatta ttgaagcaat aaaagatgct
     2761 gcgagctttc agttaataca tattacgggc aagaatcatt acgaaaggat aagaaacctt
     2821 ttggcaaaaa aaggtataga cactgaggaa ttaggaaata ttattctaag gccgtatatc
     2881 catgagatgc aggaagccta tgctgccgcc gatctggtta tttcaagggc aggagcgctt
     2941 agtatagccg aactcagtgt ttgtggaaaa cctgccattc ttattcccct ttctaccgca
     3001 gcaaatcagc atcaggattt caatgcaaaa ttcatggaga gaaatggtgc tgccgtgatt
     3061 attgatgaag acaaactgtc ggggaaaagt ttactcgaca ctattgaatc tatcattttt
     3121 gacaagcatt gtttacataa aatgtctatg gccagcaaaa acttggccat agaagatgcc
     3181 gctgaaaaaa tattagccga gataataaaa ttggtaaagt aagtgaaatg tttacttaag
     3241 ttttacattg tgttaatatg aatttgtaca agcaaaataa tataattgta acacctttgg
     3301 gatttttgca taatatatta tatacgacat tgtctggtct catggaagga gcgaaggatt
     3361 tgacagggaa ttataatcga attcatttca tcggaatcgg cggcactggc atgagcggca
     3421 tagcaaaaat acttatgaat atgggttata atgtttccgg ttctgattta aaggtatcgg
     3481 aagctttgat tcggctaaga gatgccgggg caaacgtttt tatcggtcat catgtttcaa
     3541 atgtcatagg agcagattta gttgttgttt cttctgctat tccaaaaaca aatcccgaat
     3601 acgtaaaagc tatagaatcc aatattcctg ttgtccatag ggcagatatg cttagtttgc
     3661 tgatggattc caaaaaaggc gttgcagtat caggtgccca tgggaagaca actacgacgt
     3721 caatgatatc cttgattatg gagaaaaatg gattcgatcc cactgtcata attggcggtg
     3781 aattaaatga tataggtgga aatgccacac taggaagtgg ggagttcttg gtggctgaag
     3841 ctgatgaaag tgatggctcc ttcgtgaaac ttaagccata tattgcggta attacgaaca
     3901 ttgaaaatga ccatatggat tattataaaa atatggaaaa catgaaacgt gcttttaagg
     3961 aatttgcagg taatgtaata gaggatggct ttattgtatt gggcaatgac aatgagcatg
     4021 tcagagagct ggaaaaggat attgataaag agttttatac cttcggaata aatcataatt
     4081 cagattttat gcctaaaaat ataaggcttg aaggtttatc tacccgattt gaaatatatt
     4141 ttaggggaaa attactgggg gaaatgcagc tcaatgttcc tggtatccac aatattgtta
     4201 atgccacagc agctatagct gtgggaaatc gtcttggaat aagcatagaa ggtatgaggg
     4261 aagcaataaa aactttccga ggagttcaac gacgttttca gattgttggt gacatagacg
     4321 gaataaaggt tatcgatgat tatgcccatc atccaacaga aataaaggct acgctgagag
     4381 ctgcaaaaaa ctgtagacct agaaaagttt atgccatctt tcaaccccat aggtatacga
     4441 ggactcagaa tttatcaaag gaatttggct catctttttt cgatgcggat gagattgtgc
     4501 taacggatat atatagtgcc ggcgagacac ctattaaagg agtgtcttca gatctcattg
     4561 cggagtattt aagggaaaat gggaaaaaag ttgtatatat aaaaagaaaa gaggagattc
     4621 ctgactacat tattgataag aagctcgtac ccggcgatta cattttaaca attggagcag
     4681 gagatattag cgatgtagcg tacagcatag tttctaaatt aaagtctcgt caaaaggggg
     4741 taattggcaa tattccggag gtgaaataag cggggatgta tatcattcag ggaggagaga
     4801 ctctttctgg aaaaataaag gtacagggtt caaagaattc cagccttccc atcctagccg
     4861 caactgttat caatggtaaa aaaaacatca tatgcgatgt gcccctaatt aaagatattg
     4921 aggttatgtg caaaatattg tcaaacttag gcgcaactat tgtaaatagg ggcaatgtta
     4981 tagaggttga ctcggaaaag gtgaattctt ttgaagttcc cgaacagttg atgagagaga
     5041 tgcgctcatc tattatcttg atgggtgcat tgcttggcag gtttggcagg gccaaggtgt
     5101 cctatcccgg cggatgcgaa atcggtccaa ggcctattga tttacattta aaagggctat
     5161 ccttgatggg ggtggatttt agcgaaggcc atggtttttt aaatgcaaaa accgatgggc
     5221 taaaaggggc agatatacat cttgactttc cgagcgtagg tgctacagaa aatcttatgc
     5281 ttgcaggagt ccttgcacat ggtaccacca tagttcgaaa tgcggccaag gaacctgaaa
     5341 ttgtagattt acaaaacttt ttaaaccgaa tgggagctaa tataaaaggt gcgggaacgg
     5401 atacaataaa gatagaaggc gttaaaatcc atgacttaag agcagtcaat gattacaccg
     5461 taataccgga caggattgtc gcaggaacat atctcgttgc tgccgcgatc acaagaggtc
     5521 atattatcct taccaatgtc ataactgaac acttggaacc catcctagcc aaattaagag
     5581 aaaccggctg cgtgattaac agtagaaacg gtcaggtgga attgtatgtg gataaaccca
     5641 tggtttcatt ggaaagttta agaactttgc cctatcccgg ttttcccact gacatgcaag
     5701 cgccgatgat ggccctttta gcgacagttg aagggacctc catactcacg gagacgattt
     5761 ttgaaaatag attcaagcat gcccaagaac tgaggcgaat gggtgcaaat attaccataa
     5821 atggaaatac ggctattatt aagggagtca aaaaacttac tggggccatt gttgaagcca
     5881 aggatttgag agcaggtgca gctctgattg tagcggcatt tgccgctgaa ggagagaccg
     5941 ttgtagaagg agcgtgtcac gtagaacgag gatatgaaga tatagtgggc aaactgaagg
     6001 ctttaggggc gaaaattgtc aaaaaacact agattaaata caaatatttg caaatatagt
     6061 acaattattt tgaaattact aggtttccct gtaccgacag attaccaaat agcctagtcg
     6121 ggtagggttc ttgttttttt atcaacaaaa aggacaagtg gtcatatggg aagattcggt
     6181 gaaaacagaa actataaaat agccactaga agaaaaagga taattaacta taaaagacta
     6241 ttgttgtcac tggctttttt atgtataata tcattgatgc caacgcttta tagctctttg
     6301 tttgcaataa aggacgtaca gattcgcgga aacaaaacta tctcttcaga agaaatatta
     6361 tccgtaatcg acaattacta cgatacaaat atgctcaagc ttagtgccca acaagtgaag
     6421 caagatttgc agggaactat tccaataaaa gatgtaaggg ttcactataa attaccacat
     6481 accctaattc tagaagtcga agaaagagag gttgcagcag cattacatta cttaaatagc
     6541 tttgctttaa ttgacagcca gggtattatt gtgaaatcag ttacaagact agaaaactac
     6601 agcataccga ttgttacggg gttgaaagtt gttgatgcta aaattgcaaa aaaacccgtg
     6661 tttgaagaat accccatgca ctatgaaacc ttgctagagc taatagatgc agcaaaacca
     6721 atattgcatg aactttcgga aattaacctg gttgtagatg ccaacaaaga cacatccttt
     6781 tatttatata cactggatgg ttaccaggtt tttttaggcc aatgggatga caaaaaaata
     6841 acaactatgc gagacttgtt ggcagatata agagacaaaa acttaggaaa aggtttattg
     6901 gatattagtc acaatacgcc catttttaaa cccttttcca acagcgacaa gtctggagag
     6961 gaggcaaagg atgaagaata atattaaagc ccacttcatg atagctgcca tctgtttttt
     7021 attaggcatg ttacttgtag ctcaatttag aagtgtacag ac
//
LOCUS       DAONUO010000075         6974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8179_length_6974_cov_3.832924, whole genome shotgun sequence.
ACCESSION   DAONUO010000075 DAONUO010000000
VERSION     DAONUO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_6974_cov_3.832924"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..2105
                     /gene="ilvB"
                     /locus_tag="PK768_04005"
     CDS             444..2105
                     /gene="ilvB"
                     /locus_tag="PK768_04005"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQA60162.1"
                     /translation="MKITGAEALVKSLELENVEIIFGYPGGAILPVYDALNNSKIKHV
                     LTRFEQGAAHAASGYARVSGKVGVCMATSGPGATNLVTGIATAYMDSIPIVAITGQVS
                     TSVIGRDVFQEVDITGATAPFTKHNYLVKNAKDIPRIVKEAFYIASTGRPGPVLIDLP
                     KDVAQTVIDFKYPKNIVLRGYKPNVIGHPLQVIRAAEMINDAARPLICAGGGINSARA
                     EKELKKLAEKANIPVVTTLMGTGAFPADHSLYVGMIGMHGVAPANKTVAESDLIIAAG
                     ARFADRTIGLMEGFAPKAKIIHIDIDPAEIGKNIPVDTPIVGDVKQVLSEIEKHVASK
                     DKGDWNNYVDSLKQNATYSADSKGLNPVEVIRHLSDKTKGKAIITTEVGCHQMWTAQH
                     YKFIEPRSFISSGGLGTMGYGLPAAIGAQFANGEKMVINICGDGSFQMNLQEMATAVE
                     NNLPIKIILFNNQGLGLVRQLQEFYCDKRYNQVHFRFAPDFIKLAASYGIEGLRIQKQ
                     EEISAAIDKLIEHKGPFLLECVIDINENVYPMVLNGSPINEMIGG"
     gene            2109..2609
                     /gene="ilvN"
                     /locus_tag="PK768_04010"
     CDS             2109..2609
                     /gene="ilvN"
                     /locus_tag="PK768_04010"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQA60163.1"
                     /translation="MKTTLSVLVENHPGVLSRIAGLFSRRGYNIDSLAVGTTENPEVS
                     RMTIVVEGDEYVVEQVKKQLNKLVDVIKVSKIIPEDSVTRELVLVKVDAEPQMRAGII
                     QIAEIFRANIVDVSRKTMTIELTGDKDKVQAFEELLKPFGIKELVRTGIIAINRGTNC
                     IKVEEE"
     gene            2614..3612
                     /gene="ilvC"
                     /locus_tag="PK768_04015"
     CDS             2614..3612
                     /gene="ilvC"
                     /locus_tag="PK768_04015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQA60164.1"
                     /translation="MAKMYYDNDADLQLLEGKKVAIIGYGSQGHGHALNLKESGIDVV
                     VGLYEGSKSWEVAENDGLQVKTVSEAAEEADIIMILIPDHLQGKVYEKEIKPHLKEGD
                     ALAFAHGFAIVYSQIVPPEYVDVFMIAPKGPGHLVRRTYVEGGGVPCLLAVEQDYSGR
                     AKDIALAYAKGIGGTRAGVIETSFTEETQTDLFGEQCVLCGGVSELIKAGFETLVEAG
                     FQPEIAYFECLHEMKLIVDLIYEGGLSLMRYSVSDTAKYGDFTVGKRIITEETRKEMK
                     KVLDEIVSGKFAKDWILENQAGRPVFRAIYASEAEHQIEKVGKELRSMMPWLKKKK"
     gene            3656..5191
                     /locus_tag="PK768_04020"
     CDS             3656..5191
                     /locus_tag="PK768_04020"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQA60165.1"
                     /translation="MSRRIEIFDTTLRDGEQTPGISLNVKEKLEVARQLEKLSVDVIE
                     AGFPIASKGDFNAVKTIAENVRGPVIAGLARANFKDIDCAAEALKAAARPRIHTFIAS
                     SPIHMKYKLKMTEEEVLEAAVAAVKRAKSYVEDVEFSAEDASRSDVEFLCRLYEAAIK
                     AGATVINVPDTVGYTTPREFGKLIRALMERVPGIENVKLSVHCHNDLGMAVANSLEAV
                     ACGATQIECAVNGLGERAGNAALEEVVMALDTRKDFFGFTHNINTKQIYRTSKLVSTL
                     TGVAIQPNKAIVGANAFAHESGIHQHGVLAEKSTYEIMTPESVGLTTNRLVLGKHSGR
                     HAFAQRLKELGYELAQDELNKAFERFKDLADKKKEITDMDIEAIVEDQVLKWPEHIEL
                     EYFQVSTGNQSIATATVKVKIDEEVVEEASTGDGPIDAAYKALERACNINCKLVDYSI
                     RSVSSGKDALGEVTVKVEKDGRTFLGRGLSTDIIEASVIAYTNALNKTMFSNGNGKAR
                     EVI"
     gene            5192..6466
                     /gene="leuC"
                     /locus_tag="PK768_04025"
     CDS             5192..6466
                     /gene="leuC"
                     /locus_tag="PK768_04025"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQA60166.1"
                     /translation="MGMTITQKILAKHTGRSFVEPGELIMVDVDLALANDITGPVSIE
                     EFKKAGADKVFDRSKIALIPDHFAPNKDIKSAELCKNLREFAKEQQIEHYYEVGRMGI
                     EHALLPEQGIVLPGDVVIGADSHTCTYGALGAFSTGVGSTDLAFAMMTGKCWFKVPES
                     MKFIFHGKLNPWVTGKDLILHTIGDIGVDGALYKAMEFTGEALSQLTMDERLTMTNMA
                     IEAGGKNGIMEPDEVTLEYVKNRAKRPYEVFKSDEDASYSEIIDYDATKIGPKIALPH
                     LPENVVDVKEAQGIAIDQVVIGSCTNGRLEDMRMAAKVIKGHKVHPYMRLIVIPATQQ
                     VYRDMIKEGITEIFIEAGAVVSTPTCGPCLGGYMGILAKGERALSTTNRNFVGRMGHP
                     ESEIYLSNPAVAAASAVTGKITHPEEVARDDI"
     gene            6456..6947
                     /gene="leuD"
                     /locus_tag="PK768_04030"
     CDS             6456..6947
                     /gene="leuD"
                     /locus_tag="PK768_04030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQA60167.1"
                     /translation="MIFKGKAWTFKDNIDTDVIIPARYLNTTDPKELAAHCMEDVEPE
                     FSKKVAEGDIIVAGKNFGCGSSREHAPLAIKSSGVSCVVAKSFARIFYRNSINIGLPI
                     LECEECVDEVSAGDELEIDTDKGVISNLTTGKTYKAKAFPEFISEIINAGGLLGYAKK
                     VGK"
BASE COUNT         2076 a         1482 c         1875 g         1541 t
ORIGIN      
        1 tagggtgccg gccgataaag cgtctgcgac cgagcggtac aatctaaggt tttccttaga
       61 acaccgtgag aataaaaatc tccagcggca ggttcctatg tggaatttcc cgttgggttt
      121 tttattataa aaatacaaat ggccatatta cggaaatgta atgatggaga gtatggcaaa
      181 gaaagtggca cagagagccg gacaaggtga aagccggtcc accgtatttg ttcattctcg
      241 ctcctgaaca gttcgggcga aatacgagta gtccgagacc gcagcattgc gttaaaatga
      301 agtagcaatc tacttcccga ggccgagacc gcaaggtctt aggcaaaaca gggtggtacc
      361 gcgaggaaac ccctcgtccc tgagaaagat tctcaaggcg aggggttgtt ttatatcatt
      421 ttacaataag gtggtgactt aaattgaaaa ttactggagc tgaagcttta gttaaatccc
      481 ttgaactcga aaatgtagaa attatctttg gatatcccgg gggtgctata cttccagtat
      541 acgatgccct caacaattcg aaaataaaac atgttttgac ccgctttgaa cagggagctg
      601 cccatgcggc cagcggctat gcccgcgttt caggcaaggt aggcgtgtgc atggccacat
      661 caggcccagg tgctacaaac ttagtgacgg gaattgccac ggcatacatg gactccattc
      721 ccatcgttgc aataaccggc caggtatcta caagcgttat aggcagagac gtctttcagg
      781 aagtggatat taccggtgcc acggcaccct ttaccaagca caactacctc gtgaaaaatg
      841 ccaaagacat tccccgcata gtcaaggaag cattttatat cgcttcaacg ggcaggccgg
      901 gtcccgtgtt aatagacctg ccgaaggatg tcgcacaaac ggtaatagat ttcaagtatc
      961 ctaaaaatat cgtgttaagg ggatacaaac ccaatgtgat aggccatccg ctacaggtaa
     1021 tccgggcagc cgagatgata aacgatgcgg caagacccct gatttgtgca ggtgggggca
     1081 ttaattctgc aagagctgaa aaagagctga aaaagcttgc cgaaaaggca aatatccccg
     1141 ttgtgacaac gctgatggga acgggagcct ttccggcaga tcattccctc tatgtgggca
     1201 tgattggcat gcacggagta gctccggcca acaaaactgt agccgaatcc gatttaataa
     1261 ttgcagctgg agctcgattt gcggatcgaa ccatcggcct gatggagggc tttgctccga
     1321 aggccaaaat aatccacatt gacatcgatc ccgcggagat tgggaaaaat ataccggtag
     1381 atacgccgat tgtgggagat gtaaaacagg ttttatcgga aatagaaaaa catgttgcct
     1441 caaaagacaa aggggactgg aacaactatg tggatagctt gaaacagaat gcgacatata
     1501 gtgcagatag caagggctta aaccctgttg aagtaatccg ccatctttcg gacaaaacca
     1561 agggcaaggc cattattaca acggaagttg gctgccatca gatgtggact gcccaacact
     1621 ataagtttat tgagccccgc agcttcattt catccggcgg cctcggcacc atgggctacg
     1681 gccttccggc agcaatcgga gctcaatttg caaacgggga aaagatggta atcaatattt
     1741 gtggcgatgg aagttttcag atgaacttgc aagaaatggc caccgccgtg gagaataatc
     1801 ttcccataaa aataattctt tttaacaatc agggtttagg gctggttcgt caactgcagg
     1861 agttttactg cgataaacgc tacaaccagg tgcactttag atttgcgcct gacttcataa
     1921 agcttgcagc aagttatggg atcgaaggtt tgaggattca aaagcaggag gaaatatcgg
     1981 ctgctataga taagctgata gagcataagg gtcccttcct gcttgaatgt gtaatagaca
     2041 tcaatgaaaa tgtatatccc atggtattaa acggttctcc catcaatgag atgataggag
     2101 ggtaaggcat gaaaacgaca ctttcagtac ttgtagagaa tcatccaggt gtactatcta
     2161 gaatagcagg gctttttagc cgaaggggct ataacatcga cagcctggct gttggaacca
     2221 ccgagaatcc ggaagtctcc aggatgacca tagtggttga aggcgacgaa tatgtagttg
     2281 aacaggtaaa aaaacagctg aacaagctgg tggatgtcat caaagtgagt aagatcattc
     2341 ccgaagattc ggttacgagg gaactggtct tggtcaaggt ggatgccgaa cctcaaatga
     2401 gggcaggaat tatccaaatt gccgagatct tcagggccaa tatcgtagat gtgagccgca
     2461 agacaatgac gatagagctc acgggagata aagataaggt ccaagccttt gaagaactct
     2521 taaagccctt tggcataaaa gaactggtca gaactggcat tattgccata aatagaggaa
     2581 caaattgtat aaaagttgag gaggaatgat aacatggcaa agatgtatta tgacaacgat
     2641 gctgatttac agttacttga gggaaagaag gttgcaatta tcggctacgg cagccagggc
     2701 cacggccacg cccttaactt gaaagagagc ggcattgatg tggttgtagg cctctatgaa
     2761 ggcagcaaat cctgggaagt tgccgaaaat gatggcctcc aggtaaagac cgtcagcgag
     2821 gcagcagagg aagcagatat tataatgatc ctaattccgg accacctgca gggcaaggtt
     2881 tatgaaaaag aaataaagcc ccatctcaag gagggggatg cactggcctt tgcacacggc
     2941 tttgcaattg tctacagcca aatcgtgccg ccggaatatg ttgatgtctt catgatagca
     3001 ccgaaggggc ccggccacct tgtcaggcgg acatacgtag agggcggcgg cgttccatgc
     3061 ctcctagcgg ttgaacagga ctattcgggc agagccaagg atattgccct ggcttacgca
     3121 aagggcatag gaggcacaag agcaggagta atagagacta gctttaccga ggaaacccag
     3181 acggatctat tcggagagca gtgcgtcctc tgcggcggcg tatcggagct cataaaggca
     3241 gggtttgaaa ctttagttga agcgggtttt cagcccgaga tagcgtattt tgaatgcctc
     3301 catgaaatga agttaatcgt tgacctgata tatgaaggcg gtctcagcct tatgagatac
     3361 tctgttagcg acactgccaa gtacggagac tttaccgtag gcaagcggat cattaccgaa
     3421 gagacccgca aggaaatgaa gaaagtcctt gatgaaattg tcagcggaaa attcgccaag
     3481 gactggattt tagagaacca ggccggcagg ccggtgttca gggccatcta tgcgagtgaa
     3541 gcagaacacc agatagaaaa ggtaggcaag gaactcagaa gcatgatgcc ctggctcaaa
     3601 aagaagaaat aagttaataa cataggtgcc aggcatcgga aaagaggtga ggatagtgtc
     3661 tagaaggatt gaaattttcg atacgacact tcgcgatggg gaacagaccc ccggcatttc
     3721 attaaatgta aaggaaaagc tggaagtggc caggcaactt gaaaaacttt cggtggatgt
     3781 aatagaagca ggctttccca tagcttccaa gggcgacttc aatgcagtaa aaaccattgc
     3841 tgaaaacgta agagggcccg taatagcggg cctggccagg gccaacttca aagatataga
     3901 ttgcgccgcc gaggctttaa aggcagcggc aaggccaagg attcatacat ttatagcttc
     3961 atctcccatc catatgaaat ataaactgaa aatgactgaa gaagaggttc ttgaagcggc
     4021 ggtagccgcc gtaaagaggg ccaaatccta tgtggaagat gtggagtttt ctgcagagga
     4081 cgcatcaagg tcagatgtgg agtttctctg caggctctac gaggctgcca taaaagccgg
     4141 agctacggtc ataaacgttc ctgatacggt gggatacaca acgcccaggg agttcggtaa
     4201 gctgatcagg gctttaatgg aaagggtgcc cgggattgaa aatgtaaagc ttagtgttca
     4261 ctgtcacaat gatttgggca tggccgttgc caattccctg gaagccgtag cttgcggtgc
     4321 aacccagata gaatgtgcag taaacggcct cggggaacgg gccggcaatg ccgccctgga
     4381 ggaagtggta atggcactgg atacccgcaa ggactttttt ggattcaccc acaacataaa
     4441 tacaaagcag atataccgta ccagcaaact ggtaagcact ttgacgggtg tagctatcca
     4501 gccgaataaa gcgatcgtgg gggcgaatgc ctttgcccat gaatcgggga ttcatcagca
     4561 cggcgtgttg gctgaaaaat ccacctatga aatcatgaca ccggaatccg taggtcttac
     4621 caccaacagg cttgttctgg gcaagcattc cggcaggcat gcctttgccc agagattaaa
     4681 agagctgggc tatgagttgg cccaggatga attaaataag gcctttgagc gctttaagga
     4741 tcttgcggac aagaagaaag aaatcactga catggatatt gaagctatag tcgaggatca
     4801 ggtgttgaaa tggcccgagc atatagaact tgagtacttc caagtttcta cgggaaatca
     4861 atccattgcc acagcaacgg ttaaagttaa gatagatgaa gaagtagtgg aagaggcgtc
     4921 aacgggcgat ggcccaatag atgctgcata caaggcctta gagcgggcct gcaatataaa
     4981 ctgcaagctg gttgactatt ccatccggtc cgtatcaagc ggcaaagatg ccctgggaga
     5041 ggtcacggtc aaggtggaaa aggacggcag gaccttttta ggtcgtggtc ttagcaccga
     5101 tatcatagag gcaagtgtaa ttgcatacac caatgcgtta aacaagacaa tgttcagtaa
     5161 cggcaatggc aaagcacggg aggtgattta gatggggatg accattaccc aaaaaatcct
     5221 ggcaaagcac acgggcagga gcttcgtgga accgggggaa ctcataatgg tggatgtaga
     5281 cctggccctg gccaatgaca taaccggtcc agtgtccata gaagagttta aaaaagccgg
     5341 tgcagacaaa gtctttgaca ggagtaaaat cgcactcatt cccgaccact ttgcaccgaa
     5401 taaggatata aagtcggccg agctttgcaa aaacttaagg gagtttgcaa aggagcagca
     5461 gatagagcac tactacgagg tcggccgcat ggggatagaa cacgcccttt tgcccgagca
     5521 gggcatagtg ctcccgggag atgtagtgat cggtgcagat tcccatacct gcacgtacgg
     5581 ggctttgggc gctttttcca ccggagtcgg ttccaccgac ttggcatttg ccatgatgac
     5641 gggcaagtgc tggtttaaag tgcctgaaag catgaaattt atctttcacg gcaaattaaa
     5701 tccctgggtt accggaaagg acttgatatt gcatactatc ggcgacatcg gtgtggacgg
     5761 tgccctttat aaagccatgg aatttaccgg cgaggccctt tcccaactga ctatggatga
     5821 gcggcttacc atgaccaata tggccataga agctggcggc aaaaatggaa tcatggaacc
     5881 cgatgaggtg accctggagt acgtcaaaaa tagagcaaag cggccttatg aagtttttaa
     5941 gagcgacgaa gatgccagct actcggaaat aattgattat gatgcgacga aaatcgggcc
     6001 taagatagca cttccccacc ttccggaaaa tgtcgtggat gtcaaggagg cgcagggcat
     6061 tgcgatagat caggttgtta ttggctcctg taccaacggc aggctggagg acatgaggat
     6121 ggctgcaaag gtgataaaag gccacaaggt ccacccctat atgagattga tagttatccc
     6181 tgctacccag caggtttaca gggacatgat aaaagaagga attacagaaa tctttatcga
     6241 ggcaggtgcc gtggtgagca cacccacctg cgggccatgt cttggaggtt acatgggcat
     6301 cctagccaaa ggcgagaggg ccctgtccac caccaacagg aattttgtag gcaggatggg
     6361 gcatcctgaa agcgaaatat acctgtcaaa tcctgcggtg gcagcagctt cagctgtaac
     6421 gggaaagata acccatcccg aggaggtggc cagagatgat atttaagggc aaagcctgga
     6481 cattcaaaga taatatcgac accgatgtga tcattcctgc ccgctatttg aatacaacgg
     6541 atccaaagga gctggcagct cattgtatgg aggatgtgga accagaattt tcgaaaaaag
     6601 tggctgaagg cgatatcata gtggcaggca aaaactttgg ctgcggcagc tccagggagc
     6661 atgcaccttt ggccataaaa tcctctggcg taagctgtgt agtagcaaag tccttcgcga
     6721 ggatatttta cagaaattcc atcaacatag gcttgccgat acttgaatgt gaagaatgcg
     6781 tagatgaggt atcagccggg gacgaacttg aaattgacac ggacaaaggt gttatatcta
     6841 acctgacaac gggtaagaca tataaggcta aggcctttcc cgaattcata agtgaaataa
     6901 tcaacgccgg aggcctttta ggttacgcaa agaaggtggg taaataatga acaaagtagc
     6961 ccttctgccg ggag
//
LOCUS       DAONUO010000076         6967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8186_length_6967_cov_3.740885, whole genome shotgun sequence.
ACCESSION   DAONUO010000076 DAONUO010000000
VERSION     DAONUO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8186_length_6967_cov_3.740885"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1179
                     /locus_tag="PK768_04035"
     CDS             67..1179
                     /locus_tag="PK768_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HQA60168.1"
                     /translation="MGHDLTQSIRKIVEENSDIKGKNIMITASHTHSGPAASRMEGNY
                     SWMNIKGSEIDEAYYTLLRENIARAILWANNELEEVAIGTGKGSLVGLGSNRQDPNKP
                     FDHEVNVLRVDSKRGDQPIAVIVNYPCHPTVLSAENYLISGDFPSYMAKGISRFFPGC
                     EAMFMQGAAGDISTRHNRRESTFKEAKRMGEMLAGEVIKVMNQMCMTDEIDLTSFLLP
                     LQIPVREFEADEVCVKKIEDAKKALRRLQEEGAPENIVRTAIVNLQGAERYYVLKQSL
                     NFREFNTEMQLFKLGKTAIVAVPAELFNKIGQEIKSLSGKYNVIVAGYANDYVGYVLD
                     AESYGEDSYEAGVSMVHKDAEKIIVNTAKELISMIR"
     gene            1218..1946
                     /locus_tag="PK768_04040"
     CDS             1218..1946
                     /locus_tag="PK768_04040"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF002231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HQA60169.1"
                     /translation="MLEKLKNGLIVSCQAHESEPMHGSIFMAAMAKAAEMAGAVGIRA
                     NGPENIRAIRAAVKLPIIGIYKIRNPLYSVYITPTVEAAIAIKEAGADIIAIDCTFNE
                     RESGLTAAEMIRATKRITGLPVMADISTCEEGIAAEEAGADLVATTLAKAKKTDESYD
                     DEQGGYKPIVEHAPPSLDLISELCCNVRLPIIAEGRFWEPEQVVEALKRGAHAVVVGT
                     AITRPSEITHRFVRAINKYINDIQ"
     gene            2019..2432
                     /locus_tag="PK768_04045"
     CDS             2019..2432
                     /locus_tag="PK768_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQA60170.1"
                     /translation="MVGIVIASHGPLASCMMKSAELICGSNSQIAAVSLTLEMSLEEF
                     HKKLISAVKQVDCGEGVLVLADIPGGSPSNISLQESAKAENVEVVSGFNLGMLLEILI
                     NREGKNPMELAQFACEAGKKSIEFLSKKLKDMTAG"
     gene            2496..3227
                     /locus_tag="PK768_04050"
     CDS             2496..3227
                     /locus_tag="PK768_04050"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQA60171.1"
                     /translation="MSVPERYFDFVISLMEEIKNTQKENIIKAAELVADSVMKGGRFY
                     VFGTGHSHMVAEEIYLRAGGAAFVKAILEPSLMLHEMPNKSTYLERLEGYAEVLLRLH
                     KVDEKDTLMVVSNSGRNAIPVEMALGAKKLGASVIALTSLKHSSLVTSRHKSGKKLYE
                     IADVTIDNCGEMGDAVFEIDGLKTATGPTSDSTGTAIAQALIATTLDILVKRGYESPV
                     FVSSNVDGADEINDRLFDKYYGFIK"
     gene            3239..4603
                     /locus_tag="PK768_04055"
     CDS             3239..4603
                     /locus_tag="PK768_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQA60172.1"
                     /translation="MKGIILAQGLGKKMWPYSDHWQKCALPVGNKGNILRLTDILETL
                     GVEKIKIVIAYKSAQVKYLLKDKNLEYIEAPINGTADALLAAIEEQEASIVVYGDIIV
                     DKEDISRCVDKFNDTKCPVMLYKPLDGGEQSIDWICCNVAEDTVLSLYGHPRPHYVNA
                     RVGGVFVLDKRVIPYLKANPGYMKNVPVGGMPPEQKELEQSLQMMIEDGEKILGVKAR
                     YFLIDLDKPWHLMDANKAIIERDVAGLAEDDIHPSAKVHPSAIIEGKIRIGENSYIGK
                     NVIIKGNVIIGNNTSIDYGAIIEGNVIVGNNTAINDYCKIASYTTIGDWNRIGYNAEV
                     QGITFEGVSITHGCEIYGVVGQRTDIAAGCLVGVLRFDDGFSTPRIKGRRELGNPHTN
                     AVFLGDHSRTGVGNIFFPGVRVGKNCAIGPGAIVSEDIPSGSLVIVRQSQEIKPWGPN
                     RYGW"
     gene            complement(4737..5981)
                     /locus_tag="PK768_04060"
     CDS             complement(4737..5981)
                     /locus_tag="PK768_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA60173.1"
                     /translation="MSDIRPLKVFVIGGGASGMMAALAARYKGADVTILERNPRVGKK
                     ILATGNGRCNYTNVNLSANCYHGKNPDFVYGALSAFDVSKTIGFFEKLGIAPRVEEGG
                     KVFPMSGQASSVLDLLRYELEKTGVNVICEAFVKDVKKTKKGFRLQLEDGRSLEADRV
                     ILACGGKAMPSTGSDGNGYDLARKLGHKIVDIFPALVQLKLEGNFFKQIDGVKFVGTA
                     EVLSGGKALARDRGDILFANYGVSGPPILQISRKAGELLQEKKEAILKISIIDDMGRK
                     ELDGFLEKRIKHMPERSLDFSLVGFVNKRLIPVLLKESGIENIKMPVRELSAQDRGKI
                     ISILTDWSFKIRGTKSWPSAQVTAGGVDTDQIDPFTMESALVKGLYFCGEIVDIDGAC
                     GGFNLQWAWSSGYVAGSSAASR"
     gene            6439..>6967
                     /locus_tag="PK768_04065"
     CDS             6439..>6967
                     /locus_tag="PK768_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60174.1"
                     /translation="MENQGKTSFTLLAISKLYNTRVNLVGIDENHRWIRPMPVYQSDI
                     FAQEEKIFEVFGVTELVLNDWWGTAPRPEDRFFVRNPQVLPRLVKVLDETERVKLLRS
                     LVDDSVDSIFSRGRTLGLIKAVVKDLNFRRNPYNPLEYEARMVFEDILGNLNYNWMVT
                     DFMWHKSFQDFIRENP"
BASE COUNT         2003 a         1518 c         1731 g         1715 t
ORIGIN      
        1 tactaactct ctttaggaga accttcttat tgcagacttc gtggaatttc gagcgacctg
       61 tcgggggtgg gccatgacct tacgcaaagc atcagaaaaa tcgtggaaga aaactcggac
      121 ataaagggca aaaacatcat gatcaccgca tcacatacac attcgggacc agccgcctct
      181 agaatggaag gcaactatag ttggatgaat ataaaagggt cggagataga tgaggcatat
      241 tatacactgc tcagagaaaa tatagctagg gccatactgt gggccaacaa tgagctcgaa
      301 gaggttgcga ttggaacggg caagggttcc ttggtcggcc ttggttcgaa ccgccaggat
      361 ccgaataagc cctttgacca tgaagtcaat gtattgcgcg ttgacagcaa gaggggcgat
      421 caacccattg ccgtaattgt caactacccc tgccacccta ctgtactgtc ggcagagaac
      481 tatcttattt cgggggattt tccatcatac atggcaaaag ggatttcaag gtttttcccg
      541 ggatgtgaag ccatgtttat gcagggtgct gcgggagaca tcagcactcg acacaatcga
      601 agggaatcta cttttaaaga agctaagcga atgggcgaaa tgcttgcagg agaagtgata
      661 aaagttatga atcagatgtg catgaccgat gaaatagact tgacatcttt ccttcttccc
      721 ttgcaaatac cggttagaga attcgaagct gatgaagtgt gcgttaaaaa gatagaggat
      781 gccaaaaagg ctctgcgcag actacaggaa gaaggtgcac cggaaaatat agtgagaacc
      841 gccattgtga acctgcaggg cgcagaaaga tactacgtct taaaacagag tctaaacttt
      901 agagaattca atactgaaat gcagctgttc aagttgggca agaccgccat tgtagccgtt
      961 ccggcagaat tgtttaataa aataggccaa gaaatcaaaa gcttgagcgg caaatacaat
     1021 gttatagttg cgggttatgc caatgactat gttggatacg tcttagatgc ggaaagctac
     1081 ggggaagaca gctatgaggc tggagtaagc atggttcata aagatgccga aaaaataatc
     1141 gtaaatacgg ccaaggaact catttcaatg atcaggtaag tcattatagg gattcccttt
     1201 gcgaggagtg ttaataaatg ctggaaaagc taaaaaatgg cctgatagtt tcttgccaag
     1261 cacatgaaag cgaaccgatg cacggctcca tttttatggc agccatggct aaagctgctg
     1321 agatggcagg tgccgtggga ataagggcca atgggccaga gaatattcgg gcaattaggg
     1381 cagcggtaaa attgcccatt attgggatat ataagattag aaatccttta tattccgtgt
     1441 atattacccc tacggtggaa gcggcaatag caataaagga agcaggagcg gatataattg
     1501 ccatagactg tacctttaat gagcgagaaa gcgggctcac tgcggctgaa atgataagag
     1561 ccaccaaacg aataacgggt ctgcccgtaa tggctgatat ttctacctgt gaagaaggta
     1621 ttgctgccga ggaggcaggg gcggatttgg tggctaccac attagcaaaa gctaaaaaga
     1681 cagatgagtc ctatgatgac gaacagggtg gatacaagcc tattgtagaa cacgccccac
     1741 catcgctgga tttgataagt gaactttgct gcaatgtcag gctgccgata attgccgagg
     1801 ggagattctg ggaacccgaa caagtggtag aagctttaaa aagaggtgca catgctgtgg
     1861 tagtagggac tgccataact cgaccgtcgg aaattacaca caggtttgtc agggcaataa
     1921 acaaatatat aaatgatatt caataaatac tcgccgttat tcagtcatcg atgcgagtct
     1981 actgttctgc aacgcttatc acgacgagga ggagtttaat ggttggcata gttatagcaa
     2041 gtcatggtcc actggcttca tgtatgatga agtcggccga attgatctgc ggtagcaatt
     2101 ctcaaattgc cgcggtgtct ttgaccctgg aaatgagcct tgaggaattt cacaaaaaac
     2161 tcatttcagc cgtaaaacaa gttgattgtg gagaaggcgt cctcgttctg gcagacattc
     2221 ccggaggaag cccgagcaat atttctttgc aggaaagtgc taaagctgaa aatgtagaag
     2281 ttgtttcggg ctttaacctg ggcatgttac tggaaatctt gattaatagg gaaggcaaaa
     2341 acccaatgga attggcccaa ttcgcatgtg aagcaggtaa gaaaagcata gaatttctat
     2401 caaaaaagct aaaagatatg actgcaggat aaaatccttt cgcgttgtgt cagccttttt
     2461 tttcaaaact atattgaagg agagaggtca ataggatgtc tgtacctgag agatattttg
     2521 attttgtcat ttctctaatg gaagagatta aaaataccca aaaggagaat atcataaagg
     2581 ccgcggaact cgttgctgac tcggttatga agggcgggag gttttatgtt tttggcacgg
     2641 gacattcaca catggtagcc gaggaaatat atttgcgggc tggcggtgca gctttcgtca
     2701 aggcaatact cgagccttct ttgatgctcc atgaaatgcc aaataagagc acctatcttg
     2761 aaaggctgga aggatatgct gaagtcttgc taaggctgca taaggttgac gaaaaggata
     2821 ctttaatggt ggtgtctaat tccggcagga atgccatacc ggtagaaatg gccctgggtg
     2881 ccaaaaaact gggggcaagt gtaatagccc tgacatcttt aaagcactct agcctggtca
     2941 catcgcgcca taagagcgga aagaagctgt atgaaatagc cgatgtgaca attgacaact
     3001 gcggggaaat gggagatgca gtttttgaaa tagatggtct caaaacggct acaggtccga
     3061 cgtcggattc tacgggaacc gccatagccc aggccctcat tgccactact ttagacatcc
     3121 tggtaaagcg gggctacgaa tcccccgttt ttgtaagttc caatgtagat ggtgcagatg
     3181 aaataaatga tcgtctattt gacaagtact acggctttat aaaataagag gtgatttgat
     3241 gaagggaatt atattagccc agggcttagg caaaaaaatg tggccttaca gtgaccactg
     3301 gcaaaaatgt gccttacccg ttggcaataa aggcaatatt ttgcggctga cggatattct
     3361 tgaaacctta ggagtagaaa aaataaaaat agtcatagct tacaaatcgg ctcaggtgaa
     3421 atacctgctc aaagataaaa acctagaata cattgaagca cctataaacg gcactgctga
     3481 tgccctcctt gccgctatag aggagcagga agctagtatt gtagtatacg gcgatattat
     3541 agtggataaa gaagacataa gccgatgtgt agataaattt aacgacacaa aatgtccggt
     3601 aatgctttac aaaccccttg acggcggcga acagtcaatt gactggatat gctgcaatgt
     3661 ggccgaagac acagttttgt ctttatacgg tcatccaagg ccccattatg tcaatgccag
     3721 ggtcggaggt gtttttgtac ttgataagag agtaattcca taccttaagg caaatcccgg
     3781 ttacatgaaa aacgtgcccg tgggaggcat gcctcccgaa caaaaggaat tagagcaaag
     3841 tctacagatg atgatagaag acggcgaaaa aatattgggc gtaaaagccc ggtactttct
     3901 cattgatctg gataaaccct ggcacctaat ggatgccaac aaggcaatca ttgaaaggga
     3961 tgtggcaggg cttgccgaag atgacataca tccaagtgct aaggttcacc catcggcgat
     4021 catcgaaggt aaaataagaa tcggcgaaaa cagctatatt ggtaaaaatg tcatcataaa
     4081 gggaaatgta attataggaa acaacacaag catcgactat ggagcaatta ttgaaggcaa
     4141 cgtaatcgta ggaaacaaca cggcaattaa cgattactgc aaaattgcat catatactac
     4201 catcggcgac tggaaccgca taggatacaa tgcggaggtc cagggaataa cctttgaagg
     4261 agtaagcata acccatggct gtgagatata cggggtagtc ggccagcgca ccgatattgc
     4321 agcaggctgc ctggtagggg tgctgagatt tgacgatggg tttagcacac ccaggatcaa
     4381 aggaagaagg gaattgggca atccccatac aaacgcggtt tttctcgggg accattccag
     4441 gacgggtgta ggcaacatct tcttccccgg ggtcagggtg ggcaaaaact gcgcaatagg
     4501 gcccggggcc atagtttccg aagatatccc ttccggcagt cttgtaatag taaggcaaag
     4561 ccaggagata aagccttggg gtccgaatcg gtacggctgg taaggatttt gaagtcgatg
     4621 ctgcggcatc gattattttt attctgaaca taacagggtt cttgctgccc aacagaatct
     4681 taagtagaac catcgcttgg ccttatttag tgaaaggctg cactaccctt gcctttttat
     4741 ctacttgcgg cgcttgaccc ggccacatat cctgaagacc aggcccattg gaggttgaag
     4801 cccccgcagg cgccgtctat gtccacgatt tcaccgcaga agtacaggcc cttaaccagg
     4861 gcagattcca tggtaaaggg gtctatctgg tcagtatcaa cgccccctgc ggtgacctgg
     4921 gcacttggcc aggacttggt tccccttatc ttaaagctcc agtcggtaag tatgctgatg
     4981 atctttcccc tatcctgggc cgatagctcc ctaaccggca tttttatgtt ttcaataccc
     5041 gactccttta acaggacggg tatgagccgc ttgttgacaa atcccaccag gctaaaatcc
     5101 aggcttctct caggcatatg cttaatgcgc ttttccaaaa atccatcaag ttcctttcga
     5161 cccatatcat ctatgatgga aatcttcaaa atggcctctt ttttctcctg gagcaattcc
     5221 ccggcctttc ggctaatttg caggatcggg ggtcccgaca cgccgtaatt ggcaaagagg
     5281 atatcccccc tgtccctggc cagcgccttt ccgccggaca ggacctcggc agtgcccaca
     5341 aatttgactc cgtctatctg cttgaaaaag ttgccttcaa gcttgagctg gaccagggcc
     5401 gggaaaatat cgacgatctt gtgccctagt ttcctggcca aatcgtatcc gttgccgtca
     5461 gaacctgtcg aaggcatggc cttgccgccg caggccagga tgaccctgtc ggcttcgagg
     5521 ctcctcccat cttccagctg aaggcgaaaa cccttctttg ttttcttcac atcttttacg
     5581 aaggcctcgc agatgacatt cacaccggtt ttctccagtt cgtaccgcag caggtcaagg
     5641 acgctggaag cctggcccga catgggaaag acctttccgc cttcttcaac gcggggagcg
     5701 attcccaact tttcaaaaaa gccgatggtc tttgacacat caaaggccga aagggcaccg
     5761 tatacgaagt ctggattttt gccgtggtag cagttcgcgc ttagattgac attggtgtag
     5821 ttgcagcgcc cgttgccggt ggccagtatt ttcttcccca ccctcggatt ccgctccagg
     5881 atggtcacat cggcgccctt atagcgggcc gcaagggcgg ccatcatacc ggaggccccg
     5941 ccgcctatca caaagacttt caaaggcctt atatcggaca tttatatctc tcctatgtct
     6001 ttcgttatac tttccagcca aactggatta ttcagtaagt cgcaagctaa gcgtgtaaaa
     6061 accctttgct actataaagc tatcatacca aaaaacctgc ctaatgtata cttgccgatc
     6121 tcatactgta tgtgatgcct cgggctgtct aaatagttaa gctgcccttt tattgtctta
     6181 gatcctgaat tcgagatttg ccagttgtta cttaaagttt tcatggcata tgtgcttttg
     6241 cttggtgttt ataaaatgcc atagtcaagg acctataaac ctttaatcct acaaaaagtg
     6301 ataatattta gcggcctcca ataatatatt aaaacaaccg ggattgtaag ccttgtcagg
     6361 ctttgttggt ctagcacaaa aatagactgc ggtgctgcaa atgaatgccg cagattactt
     6421 tgtaacggag gggtgcttat ggaaaatcag ggcaagacca gctttacctt gctggccatc
     6481 tccaagctgt acaacaccag ggtcaatctg gttggcatcg atgaaaacca ccgctggata
     6541 aggcccatgc ctgtatatca atcggatatc tttgcccagg aagaaaagat tttcgaggta
     6601 ttcggggtta ccgaactggt actcaacgat tggtggggca cggcacccag gcccgaagac
     6661 cgattcttcg tgagaaaccc gcaggtattg ccccggctgg taaaagtgtt ggatgaaacg
     6721 gaaagggtga aactcctgag atccctggtg gacgactccg tcgattccat tttcagccgc
     6781 ggcaggaccc tgggcctcat aaaggcagtg gtaaaggatc tgaacttcag gcgaaacccc
     6841 tataaccccc tggaatacga ggcccgcatg gtttttgagg atatattggg caacttaaac
     6901 tacaactgga tggtaactga cttcatgtgg cacaagagct ttcaggattt tatccgggaa
     6961 aatcccg
//
LOCUS       DAONUO010000077         6938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8224_length_6938_cov_3.499201, whole genome shotgun sequence.
ACCESSION   DAONUO010000077 DAONUO010000000
VERSION     DAONUO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8224_length_6938_cov_3.499201"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK768_04070"
     CDS             complement(<1..241)
                     /locus_tag="PK768_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HQA60175.1"
                     /translation="MRAGIIGIGSFLPDKVMPNSELEKIVDTTNEWIVERTGILNRRI
                     AQAHISSSDLGAEAARLAIKDANLKAEDIDMIISAS"
     gene            complement(247..1245)
                     /gene="plsX"
                     /locus_tag="PK768_04075"
     CDS             complement(247..1245)
                     /gene="plsX"
                     /locus_tag="PK768_04075"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQA60176.1"
                     /translation="MRILVDAMGGDRAPEEIVKGACIASKDFGLDVILIGNEGKIKPY
                     MNRIGCSLEIIHAEEVITNDEQPVTAIRKKKESSIVKGLKLLKEGKVQAMVSAGSTGA
                     LMAGGLFVVGRIKGIDRPAIATVFPTQNGPIVILDIGANSEVKPKNLVQFALMGSIYA
                     EGILEIPNPSVGLMNIGAEEEKGNSTIKAAYSDLKQIENVNFVGNVEPRDIFDGKVNV
                     IVCDGFTGNIFLKTLEGFGTYVFKRLKEEVKGNFTYSIGAMMLKPALNRVRASLDYSE
                     YGGAMFLGIDGVLIKCHGSSDSKAISNGIKAAYKFARANISEKLKENIELSIEKEK"
     gene            complement(1288..1872)
                     /gene="fapR"
                     /locus_tag="PK768_04080"
     CDS             complement(1288..1872)
                     /gene="fapR"
                     /locus_tag="PK768_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HQA60177.1"
                     /translation="MAKRWLTKRERQKLLLDTIENDPFLNDEELADKFDVSIQTIRLD
                     RMELKIPELRERIKAVAEQNYAKVRSIDIEEIVGEIVDLELDKRGISILETTSKMAFG
                     KTKVIRGDIIFAQANSLAIATIDAKVALTGVANIKYKLPVIVGQTLVAKAEVTRIRGN
                     KKFVFVRTYVKQQEVFRAKFILVSLDDEEKHGIL"
     gene            complement(2092..2274)
                     /gene="rpmF"
                     /locus_tag="PK768_04085"
     CDS             complement(2092..2274)
                     /gene="rpmF"
                     /locus_tag="PK768_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQA60178.1"
                     /translation="MANPKHRSSKSSARSRRSNWKLRLPSISECPNCHEPKLPHRACP
                     SCGQYRNREVIQVKAE"
     gene            complement(2292..2798)
                     /locus_tag="PK768_04090"
     CDS             complement(2292..2798)
                     /locus_tag="PK768_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQA60179.1"
                     /translation="MKLNLVSLKQAVGSVLDFDFKQENVELDLNREDILFVGPLRTKG
                     QIENLGDRIYQVTGQIEVEAKALCSRCLSATPISLNFVFSLKYSDIVSESGEEDIIPF
                     FGDEIDLYPKILEEIVLNWPSQILCSSDCRGLCPNCGANMNTTDCKCKTDNIDPRFAV
                     LKQLLKSD"
     gene            complement(2862..4052)
                     /locus_tag="PK768_04095"
     CDS             complement(2862..4052)
                     /locus_tag="PK768_04095"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQA60180.1"
                     /translation="MKVLVINCGSSSLKYQLFDMDNKSVLAKGLVERIGLNEGMLKHT
                     PAGKDKVEISDNIPNHKVAVKMMLDALLDENHGVLKSISEISAVGHRVVHGGEKFSGS
                     VRITDEVMNVLKECVELAPLHNPPNIVGIEAARELLPDVPMVGVFDTAFHQTMPNYAY
                     IYGVPYELYEKYGVRRYGFHGTSHRFVSAEAAKMLNKPIEETKLVTLHLGNGSSISAV
                     KNGKSIDNSMGFTPLEGILMGTRSGNIDPAIVPFIMEKEGLSTDEMNNYLNKKCGVYG
                     VSGVSSDFRDLEDAANNGNNRAQLALQIFAYQAKKFIGSYVAAMNGLDAIVFTGGIGE
                     NSSTTRKMICEDMEFFGIKIDEEKNQVRGKQADISAEAAKTKVLVIPTNEELMIAMDT
                     VALI"
     gene            complement(4073..5092)
                     /gene="pta"
                     /locus_tag="PK768_04100"
     CDS             complement(4073..5092)
                     /gene="pta"
                     /locus_tag="PK768_04100"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HQA60181.1"
                     /translation="MRYKEGVTKMEFIQKIRQRAKAFPKTIVLPEGTDERVLKAAEII
                     TKEKLAKVVLLGNPEELKSRATEVDLSGVEIIDPVTSSKANDYSELLYELRKHKGMTM
                     EKAGELVKDPVWYATLMVKNKDVDGMVAGAATATGDVFRPAFQVVKTAPGIGVVSSAF
                     MMIVPNCEYGSNGHLLFADCAINPNPDANQLAEIAIATAKTWKAIMDDEPRVAMLSFS
                     TKGSAQHEMVDKVVEAAKILKAQAPELLADGELQADAALVPQVASSKAPGSKVAGQAN
                     VLVFPDLNAGNIGYKLVQRLAKAEAVGPISQGLAAPINDLSRGCSVEDIVNVVAITAL
                     QANEQ"
     gene            5320..6588
                     /locus_tag="PK768_04105"
     CDS             5320..6588
                     /locus_tag="PK768_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HQA60182.1"
                     /translation="MTILNVVGVVAEYNPFHKGHLYHLDTIRKVYMPDAIMVALSGNF
                     VQRGEPSIFDKWARAEMALHAGADLVLELPVCFSTATAEIFAESAVKLLLESKVVNAL
                     SFGVEKFHEKELHYLGKLFADEPISFRNILNEHLKKGLSFPKAREMAVVEYLSAKNIN
                     LDIKLISGLLNKPNSILAVEYIKALNKHGSDVAILPVVRRGHQYHDKTLSKQYASATA
                     IRQAISSQSQDFMDKIIPHLPESTVNIISREIKQRRRPVFLQDFETLILYSLRRLRAY
                     ELKNYFDVEEGLENRLKKAAQSCGKLEQLIMQIKTKRYPASRIQRILIHILLNIPREM
                     VEGRTPQYLRVLGFTKRGAMLLKQINKNASIPVITRASQYKKLDLKSKAMFEKDLFSS
                     DIYRLAYECPDLRNGGSDFDRKVIFYNSSK"
     gene            complement(6570..>6938)
                     /locus_tag="PK768_04110"
     CDS             complement(6570..>6938)
                     /locus_tag="PK768_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal protein PDZ"
                     /protein_id="HQA60183.1"
                     /translation="GIYITTLNWKPALPLNIEINTGKIGGPSAGCMFVLEILNQLTPE
                     DLAQGRKIAGTGTISLDETIGEIGGVEQKVVAAYRAGAEIFIVPEKNAEAAMKAAKGY
                     DIEVVSVKNLDDVLNYLEEL"
BASE COUNT         1889 a         1576 c         1213 g         2260 t
ORIGIN      
        1 aggatgcaga gattatcata tcgatgtctt ctgcttttaa atttgcatcc ttgatggcaa
       61 gccttgcagc ctcggctcca aggtctgatg aactaatatg agcctgtgca attctcctgt
      121 tcagtattcc ggttctttct acaatccatt catttgtcgt gtctacaatt ttttcaagtt
      181 cagagttcgg cataacttta tccggcaaaa atgaaccaat tcctataatc cctgccctca
      241 tcttagttac ttctcctttt caatactcaa ttcgatattc tcctttaatt tttcgcttat
      301 gtttgctcgt gcaaacttgt aggcggcttt tatgccgtta gaaattgcct tgctgtctga
      361 agacccgtgg cattttatca aaacaccgtc aatgcccaaa aacattgctc ctccgtactc
      421 agaataatcc aggcttgctc tgactctgtt tagagctggt ttaagcatca ttgcgccaat
      481 ggaatatgta aaattgcctt tgacttcttc ttttaaccgt ttaaagacat atgttccgaa
      541 gccctccagg gtttttagaa aaatatttcc tgtaaagcca tcgcatacaa tcacatttac
      601 cttcccatca aaaatatccc taggttcaac attgcctaca aaatttacgt tttcaatttg
      661 ctttaaatcg gaatatgctg ctttaatagt agaattccct ttttcttctt cagcgccgat
      721 attcataagg ccaacactag gattaggtat ttctagaatc ccttctgcat aaatactccc
      781 catcaacgca aactgaacga gatttttcgg ttttacttca ctatttgcac cgatatcaag
      841 gatcactata ggcccgtttt gagttggaaa caccgtagca attgctggcc tgtcaatacc
      901 ctttatcctt cctaccacaa acaaccctcc ggccattagg gcaccggtgc tgcctgcaga
      961 taccattgcc tggacttttc cttcttttaa gagttttagt ccttttacaa ttgaagattc
     1021 ctttttctta cggatggcag tcacaggctg ttcatcgttt gtgattactt cttctgcatg
     1081 aattatttcc aaggaacagc caattctatt catatacggc tttatttttc cctcattgcc
     1141 tattaatata acgtccaggc caaaatcctt tgaagctatg catgcaccct ttacaatctc
     1201 ttcaggtgcc ctatctccgc ccattgcatc taccagtatt ctcatctttt tgcggctaat
     1261 cttttgccgc cgtcctcctt tctggcttca taaaattccg tgcttttctt catcatcaag
     1321 ggatacgagg atgaatttag cccgaaaaac ttcttgttgc ttgacataag tccttacaaa
     1381 tacaaatttc ttattgcccc ttattctggt aacttcagct ttggccacga gcgtttgacc
     1441 cactataacc ggtaatttgt atttgatgtt agcgactcct gtcagggcaa cctttgcatc
     1501 gatagttgct attgccagcg aatttgcttg agcaaatata atatctcccc ttatgacttt
     1561 tgtcttgcca aaagccattt tgcttgtcgt ttctagaatg gatattcccc tcttatcaag
     1621 ttccaggtcg actatttccc ctacgatttc ttctatatct atagagcgaa cctttgcata
     1681 attttgttcg gcaacagcct ttatcctttc ccttaattca ggtattttaa gctccatcct
     1741 atcgagacga atggtctgaa tgcttacatc aaatttatct gccagttcct catcatttaa
     1801 aaaggggtcg ttttcaattg tgtccagcaa taatttttgc ctctcgcgct ttgtaagcca
     1861 tcgctttgcc acctcaagct cacctcaatg gtagttgata ttagcacctg cttctaaaag
     1921 tatatattat attatataaa aaaaatacaa aaaatcaata cttatggtaa taaatattta
     1981 gaagaagaga ccctgccaac agcgcaaaaa gatattttat gtcttggaat cttgtcaaga
     2041 gcataaaaaa ataccttcga aatgaaggta ttttatcggc tattctattc attactctgc
     2101 tttcacttga ataacttcgc gattcctgta ctgcccgcag cttggacagg ctctatgggg
     2161 cagcttcggt tcgtgacaat tggggcattc tgaaattgag ggcaacctta gtttccaatt
     2221 gcttctccta gaacgggcac tagatttaga tgatctatgt tttgggttgg ccattactac
     2281 acctcctcca actaatcgct ttttaatagt tgctttaaca ctgcaaagcg cggatcaatg
     2341 ttgtcagttt tacacttgca atcagtcgta ttcatattcg cgccgcagtt aggacacagt
     2401 cctctgcaat cactgctgca caatatttgg cttggccagt taagcacaat ttcttctaaa
     2461 atctttgggt ataagtcgat ttcatcccca aagaacggta ttatgtcttc ctccccagat
     2521 tctgaaacaa tatcgctgta ttttaatgaa aaaacgaaat tcaggctgat tggggtggca
     2581 ctaagacacc tagagcacag tgcctttgcc tcaacttcta tttgcccggt aacctggtat
     2641 atcctgtctc ctaaattctc tatctggcct tttgtcctca gcggtcctac aaatagtata
     2701 tcttcccggt ttaaatctag ctctacgttt tcttgcttaa aatcaaaatc taaaacactg
     2761 cccactgcct gtttcaaact gaccagattt agtttcacaa gaggtcacct caagaaatca
     2821 tatcttatta tatattcagg gaaaaccggt gtcaagaaat tttaaatcaa tgctacggta
     2881 tccattgcaa tcattaattc ttcgtttgtc gggatcacaa gtacttttgt ctttgcagcc
     2941 tcagcagaaa tatccgcttg tttgccccgg acttggtttt tctcctcatc tatttttatg
     3001 ccgaagaatt ccatgtcttc acatatcatc tttctagtgg tacttgaatt ttctccaatt
     3061 cctcctgtaa atactattgc atcgagtcca ttcattgctg caacatatga accgataaat
     3121 ttctttgcct ggtaagcaaa aatctgtaaa gcgagttgag ctctattatt cccgttattt
     3181 gccgcatctt ctaaatccct aaaatcactg ctgacgccgg aaactccata aacaccgcac
     3241 tttttattta agtagttgtt catttcatca gttgaaaggc cttccttttc cattataaag
     3301 ggaactatgg ccggatcgat attgccgctg cgggttccca ttaaaattcc ttccaaaggt
     3361 gtaaagccca tgctgttatc aattgacttg ccgtttttaa cagcagatat gctggatcca
     3421 ttgccaaggt gcaatgtaac tagttttgtc tcttcaatgg gcttgttaag catttttgct
     3481 gcttcagcag atacgaagcg atgcgatgtt ccgtgaaagc cgtatcttct aacaccatat
     3541 ttctcatata attcatatgg cacaccgtat atatatgcgt aatttggcat tgtctggtgg
     3601 aaggcagtgt caaatacgcc aaccataggc acgtcaggta gcagctctct tgccgcttca
     3661 atgcccacaa tatttggtgg attgtgcagt ggtgccagtt ccacgcattc cttcagcaca
     3721 ttcatcacct catcggtgat tcttacagaa ccggagaatt tttcaccgcc gtgcactact
     3781 cgatgtccta ctgcagatat ttctgatatg ctctttagga ctccgtgatt ctcatccaaa
     3841 agggcatcca gcatcatttt aacggcaact ttatggtttg ggatattatc cgaaatttcg
     3901 actttgtcct tacctgccgg tgtatgtttt agcattcctt catttaggcc aatcctttca
     3961 accaggcctt ttgccagaac tgatttatta tccatatcaa agagctgata ttttaaagat
     4021 gaacttccac aattgataac aagaactttc aaattaaaaa cctcctatgc ttttattgtt
     4081 catttgcctg tagggcagta attgcgacaa cattcacaat gtcttccacg ctgcatcctc
     4141 ttgaaaggtc atttataggg gcagccaaac cctggcttat tgggccaacg gcttccgcct
     4201 ttgcaagcct ttgaacgagt ttgtaaccga tatttcctgc attgaggtca gggaatacta
     4261 atacattagc ttgtccagca accttgctgc cgggcgcttt cgaactcgct acttggggca
     4321 ccaaagcggc gtcggcctga agttctccat ctgccagcag ctccggagcc tgtgctttca
     4381 atatctttgc agcttctaca actttatcta ccatttcgtg ctgagcgctg cccttcgttg
     4441 aaaaagacaa catggcaacc cttggctcat catccattat tgccttccag gttttagctg
     4501 tggcaatggc aatctctgcc agttgattgg catccggatt tggatttatt gcacagtcag
     4561 caaatagcaa atggccattt gagccatatt cacagttggg aacaatcatc ataaaggcac
     4621 tagaaactac ccctattccc ggtgccgtct taactacctg aaaagccggt ctgaatacat
     4681 cgccagttgc agtagccgca ccggcaacca taccatctac atctttattt tttaccatta
     4741 aagtggcata ccagaccgga tcttttacca actccccggc tttttccata gtcataccct
     4801 tgtgtttgcg aagttcgtat aggagctcgc tgtaatcatt ggcttttgat gatgtcacag
     4861 ggtctatgat ttcaacacct gataggtcaa cttccgttgc cctactctta agttcttccg
     4921 ggtttcctag tagcacaact ttagctaatt tctcctttgt gatgatttct gctgccttta
     4981 aaacccgttc atctgttcct tctggcagca caatagtttt gggaaacgct ttagcgcgct
     5041 gccttatttt ttgaatgaat tccatttttg ttactccttc cttatatctc aaattttttt
     5101 attttaaatt agtgaattgt tattagtctg catcttaaac cttaacaatt ataccataaa
     5161 actaagataa agtttggaaa acagcatatt ttaatgtaat attttagatg atattggcaa
     5221 ataattatta tttaacataa ttattcttct atgccgaaac aaaaattcct ccttaatatt
     5281 tttttgcatt tgttatataa tagtcaaaag cccttggagg tgacgatatt gaacgtagta
     5341 ggtgttgttg cagagtacaa tccctttcat aaaggccatc tgtaccattt agacactatc
     5401 aggaaagttt atatgcctga tgccatcatg gttgctttaa gcggcaattt cgtccaaagg
     5461 ggagaaccgt caatttttga taaatgggca agagctgaaa tggctctcca tgctggggca
     5521 gacttggttc tagaattgcc agtatgtttt tctaccgcaa cagcagaaat atttgcagaa
     5581 agcgctgtaa aactactatt ggaatctaaa gttgttaacg ctctaagttt cggcgtggaa
     5641 aaattccacg aaaaagaact gcactattta ggcaaactct ttgcagatga gccaatttcc
     5701 tttaggaaca tattgaatga gcatttaaaa aaaggcctct cttttccaaa ggctagggaa
     5761 atggcagtag tagaatacct atcagcaaaa aatattaatt tagatataaa attaatctca
     5821 ggattgctga acaagccaaa ttccattttg gcagtcgagt atattaaggc attaaacaaa
     5881 catgggtctg atgttgctat cttgcctgtt gtaagaaggg gacaccagta tcatgataaa
     5941 acactgagca aacagtacgc aagtgcaaca gctataaggc aggcgataag ctctcagagt
     6001 caagacttca tggataagat tattccccat ttgccggaat ccaccgttaa cattatatca
     6061 agagagataa aacagcgtcg ccgcccagtt tttttgcagg acttcgaaac tttaatattg
     6121 tattccttac ggcgtctaag agcttatgaa cttaaaaatt attttgatgt agaagaaggt
     6181 cttgaaaatc gattgaaaaa agccgcacaa tcttgcggca agcttgaaca gttaattatg
     6241 caaataaaaa ctaaaagata cccagccagc agaattcaaa gaattttgat acatatatta
     6301 ctaaatattc caagagaaat ggtggaaggt agaacgccac aatatttaag ggttttggga
     6361 tttactaaaa gaggcgctat gcttttaaag caaattaata aaaatgcgtc catacccgta
     6421 ataacccgag cttcccaata caaaaaactc gacctaaagt caaaagccat gtttgaaaag
     6481 gatcttttct catcggatat ctatcgcctc gcatatgagt gtcctgattt gagaaacgga
     6541 ggatcagatt ttgacaggaa ggttatcttt tacaattcct ctaagtaatt caatacatca
     6601 tcaagattct taactgaaac aacttctata tcataccctt tagctgcttt catagcagct
     6661 tcagcgtttt tttcaggcac tataaatatt tcggcacctg ccctgtatgc tgccacaact
     6721 ttttgttcta cgccccctat ttcgcctatt gtttcatcca gacttattgt cccggttccg
     6781 gcaattttcc tcccttgtgc caaatcctct ggggttagtt gatttaaaat ttctaataca
     6841 aacatgcatc ccgctgaagg cccacctatt ttgcccgtat ttatctcgat attcaaaggc
     6901 agggctggct tccaattcaa ggtagtgatg taaattcc
//
LOCUS       DAONUO010000078         6915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8258_length_6915_cov_3.569971, whole genome shotgun sequence.
ACCESSION   DAONUO010000078 DAONUO010000000
VERSION     DAONUO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8258_length_6915_cov_3.569971"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..925
                     /locus_tag="PK768_04115"
     CDS             86..925
                     /locus_tag="PK768_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60184.1"
                     /translation="MRRFWMAVLIVFCYVFVFPMSARADAGPKPSLKVIVKNPPDREY
                     YLDLLVDYDGEHLYENTKREDVGHPEMYDVLKNYKENGWRPALVTGTKVPLFGSLVGT
                     RNGNVMVHDFSYVGIPDRFKIIIVTKDNDIIVSENAVERKAFRSTAYFDCSTKKLSQD
                     SVALAYITQFVATCLATLIIEGLVLILFRFDTKQNWKPFLMINVFTQLMLTASVFKAM
                     YTSGIIMGLLTYIPLELLIITIEATLFARHLVQHSKRRRIAFAITANVISFALSVMAM
                     FHY"
     gene            complement(957..1610)
                     /locus_tag="PK768_04120"
     CDS             complement(957..1610)
                     /locus_tag="PK768_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQA60185.1"
                     /translation="MKRVITVKGKGKVSAPPDWIKIMMTLKASDMDYDKTMDIAANQL
                     KELRNCLGKLGFKKEDIKTTSFHIDTSYERVKDYHGNYKSVFKGYRCSNELYIGFETD
                     SKKLGEFLSALSNCKAKPEFSIKYQIRDQKSLKEEMLKKAILDAKEKAKVIAEAAGVE
                     IGNILRIDYDWSEIRFQREESYYLSEASYQPSIASIDIEPDDIKISDTVTVVWEIKG"
     gene            complement(1637..2335)
                     /locus_tag="PK768_04125"
     CDS             complement(1637..2335)
                     /locus_tag="PK768_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60186.1"
                     /translation="MKAILQMIKSEFPDKAIDISESMQLLQEMINNIMEDISNKINTA
                     FQNRDFDNVNKYQKIAENIHLYEKKIEEIIDILDIENTFTEGEIDEDTERKLIPNYAD
                     YIVDHNIEHSLYENLTHKRPFAFSINDSKIEVKTWKEMFIKTCEYLICIDEQEFMSFE
                     NNPKMNGKKNKYFSMNPNSMNDPKPVLNKIYVETNQSANSLRNLIVKMLKEFNLKISD
                     YKIYFRADYTELNK"
     gene            complement(2402..2875)
                     /locus_tag="PK768_04130"
     CDS             complement(2402..2875)
                     /locus_tag="PK768_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60187.1"
                     /translation="MIIVALNMTRDNGTIKLVGRDELKKISSQALDILKMAANQKEYT
                     IIVNTRKRNLEFTKHLPDNDYVVRTASLIAKGPIKYRTLLSKIRSIREEIGNPLLTAT
                     SIWESGIIHYAKDMMEKRNLVMLSTEHYKEISRWAGRSESLWFSTKRLITNFQII"
     gene            complement(3216..3770)
                     /locus_tag="PK768_04135"
     CDS             complement(3216..3770)
                     /locus_tag="PK768_04135"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQA60188.1"
                     /translation="MFDFSKREKILLGLLLLLTISTVSVSYYAFFKKEPDIAFKLQDE
                     PAVPVTAPAYEAEKIVIHIAGAVKNPGVYTLEEGQRVKDAIEIAGGPLPEADLLRLNL
                     AQKIHDEDKLYVPKIGESPEQSEQEDELYGSTVGIISKNDGKVNINTANETELTTLPG
                     IGPATAQKIIDYRTTSGPFKSIKK"
     gene            complement(3791..5332)
                     /locus_tag="PK768_04140"
     CDS             complement(3791..5332)
                     /locus_tag="PK768_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HQA60189.1"
                     /translation="MTAKCIMVQGTGSSVGKSRLVTGLCRVFKQDGFKVAPFKSQNMA
                     LNSYITKEGLEMGRAQATQAEACGIEPSVFMNPVLLKPYTDTSSQVVVKGRPFGSLSA
                     MDYHDYKVKLLHVVKECYDELARDNDIIVIEGAGSPAEINLREKDIVNMGMAELVDAP
                     VVLVGDIDRGGVFAALAGTLLLLSEEEKARVKGVIINKFRGDLKLLEPGLEMLEDIIK
                     RPVIGVVPYTDIYIDEEDSPETERYRLLKKYKVESNESLQQKPLQVKILSLPRITNFT
                     DFVPLAEFEGVKISYVSVGPIEDADLLIIPGSENPKESIEFIKSRGWDKDINRLYHKG
                     AVVLGIGSSFALLGKTFKTTSSDSTIEGLGLFGVHIKLLPQEPTRVKGEVASSLPDFA
                     SILSKAQLEGYRIYSGEIIKEDEGNIEFIKFKSKGHVDFDGLISQTGRVLGTFIHGIF
                     DNTDFTQQFINYLVSKRLNDSIIFETHSFNFKNYKEAEYDRWADVVRNSLDIDMLYRI
                     MGIKE"
     gene            complement(5329..6252)
                     /locus_tag="PK768_04145"
     CDS             complement(5329..6252)
                     /locus_tag="PK768_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HQA60190.1"
                     /translation="MKLNAIETELLKQIADLHKIPTGAVNIRKNGEGIIRHSSANIEV
                     KPKSDKPGIEIIVAPGTKNEAVHIPVMITMSGVKDVVYNTIIIGEGADVTVVAGCGIH
                     NSGSEKSQHDGIHDIIVKKGARMRYIEKHYGQGEGKGEKVLNPKTLLTMEENSIVEME
                     LVQIKGVDNTVRDTRAKLAERASLLVTERLLTDGSQYAVSDMTIELVGKDSNAQIISR
                     SVAKDNSKQIFRPVIIAYEKSKGHVECDSILMDQGKISSTPAITAEHPDAQLTHEAAI
                     GRIAEEQLLKLMTLGLTEEEAEDVILRGFLQ"
     gene            complement(6233..6913)
                     /locus_tag="PK768_04150"
     CDS             complement(6233..6913)
                     /locus_tag="PK768_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA60191.1"
                     /translation="MLKDINLELEDNKFYALTGPNGGGKTSLAKVIMGIYKNNRGKIF
                     YNGSDITSLSITERAKAGISYAFQTPPRFKGIKIKDILRISADESSLRARLRAVGLCP
                     QDYLDRECDSSLSGGEMKRIELATVLSRPSKVVIYDEPEAGVDLWSFEKLLQLIRKHH
                     EKGNVISIVITHHERVLSLADEIILLADGKIVDRGPCDKMLPMIRPDSRCCWQENCGG
                     SEDEIECY"
BASE COUNT         1844 a         1492 c         1190 g         2389 t
ORIGIN      
        1 caaataacac tattttttat tgttgccact tattgagggt tttatgatat cattgaattg
       61 tataactatc gcaggagtgg ttggcgtgag aagattctgg atggccgttc ttattgtgtt
      121 ttgctatgtc tttgtttttc ctatgagcgc aagagccgat gctggtccaa agccttccct
      181 taaagtgata gtaaaaaatc cacctgatcg cgaatactat ctcgacctgt tggtggacta
      241 tgacggtgag catttatatg agaataccaa gagagaagat gtagggcatc ctgaaatgta
      301 tgatgtgcta aaaaactata aagaaaacgg ttggcggcct gctcttgtta ccggtactaa
      361 agttcctctt tttgggagtt tagtaggaac tagaaatgga aatgttatgg tacatgactt
      421 cagttatgtg ggtattccgg ataggtttaa aataattata gtaacgaaag ataatgatat
      481 aatagtatcg gaaaatgcag ttgaaagaaa agcatttcga agcaccgcat acttcgattg
      541 tagtactaag aaactatcac aagactctgt cgctttggca tacattactc agtttgtcgc
      601 gacctgtctt gcaacgctta taatagaagg tcttgtcctt atcttgttta gatttgatac
      661 caaacaaaac tggaagccct ttttaatgat aaatgttttt acacagctta tgctgacagc
      721 gagtgtattt aaagccatgt atacctcagg aataataatg ggactgctaa catatatccc
      781 ccttgagctt ctcatcataa cgattgaagc tactttgttt gcaaggcacc tagtccagca
      841 ctcgaaacga aggcggattg catttgccat aacagcaaat gtcatcagct tcgcactaag
      901 tgttatggcg atgtttcatt attaaggcca tggtctgaaa ctaaaaagat gcctaattag
      961 cccttaattt cccacacaac agtgactgta tctgatattt ttatatcatc aggctcaata
     1021 tcaattgacg caattgaagg ttgatatgag gcttcgctca gataatacga ttcttctctt
     1081 tgaaaccgaa tttcactcca atcataatcg attcgaagta tatttccaat ttccactcct
     1141 gctgcttctg ctatgacttt tgccttttcc ttggcatcta aaattgcctt tttcaacatt
     1201 tcttctttca gagatttttg atccctaatt tgatacttga tggagaactc cggtttcgct
     1261 ttacagttag ataatgcaga taaaaactcg ccaagcttct tgctatcagt ttcaaatcca
     1321 atatatagtt cattagaaca tctataaccc ttgaaaacac ttttataatt accatggtaa
     1381 tctttcacac gctcataaga tgtatcgata tgaaaagatg tagtttttat gtcctctttt
     1441 ttaaacccca gcttgcctag acagtttcta agttctttta actgatttgc agctatatcc
     1501 attgttttat cgtaatccat atccgatgct ttcaaagtca tcataatctt aatccaatcc
     1561 gggggtgctg aaacctttcc tttgcctttg accgttatca ctctcttcat gataaaatac
     1621 ctccgaaaaa agtatgctat ttattcaatt cagtataatc agccctaaag taaatcttat
     1681 aatcactaat ctttaggttg aattctttca acattttaac tatcaaattt cttaaactat
     1741 tagcactctg atttgtttcg acatatatct tatttagtac gggttttgga tcgttcattg
     1801 aattaggatt catcgaaaaa tatttattct tttttccgtt catttttgga ttgttttcaa
     1861 agctcataaa ttcttgttcg tcaatgcaaa taagatattc acatgtttta ataaacatct
     1921 ctttccatgt tttaacttct attttagaat catttatact gaatgcaaag ggccttttat
     1981 gtgttaaatt ttcatataaa gaatgttcta tgttgtggtc tactatgtaa tccgcataat
     2041 taggaattaa ctttctttct gtatcctcat caatttctcc ctctgtaaat gtgttttcaa
     2101 tatctaaaat atcaataatt tcttctattt tcttttcata taagtggatg ttttccgcta
     2161 ttttctggta tttatttacg ttatcaaaat ctctattttg aaatgcagta tttattttgt
     2221 tacttatatc ttccattatg ttattaatca tttcctgtag caattgcata ctttcagata
     2281 tatctatagc tttgtcggga aattcacttt taatcatctg caaaatggcc ttcatctcac
     2341 tcccccgttt cattttagaa tagaaatgaa tttaaaaaat cacctctcat ttacatctaa
     2401 ttcatattat ttggaaatta gtaattaatc ttttggtact aaaccataaa ctttcagacc
     2461 taccagccca tcgagaaatt tctttatagt gttctgtaga taacataact aaattccttt
     2521 tttccatcat atctttcgca taatggatta taccgctttc ccatatggaa gttgctgtta
     2581 acaaaggatt ccctatttct tctcttatag acctaatctt agatagaagg gtcctgtatt
     2641 taattggacc tttagctatc aagctagcag ttctaacaac ataatcatta tctggcaaat
     2701 gcttagtaaa ttccaagttt ctttttcttg tattaacgat aatagtatat tctttttgat
     2761 tcgctgccat ttttaaaatg tcaagcgctt gacttgatat ttttttcaac tcgtctctac
     2821 caacaagttt aatggttcca ttatcccttg tcatgtttag tgcaacaatt atcatacata
     2881 attttgtttt taaactccca tacgttatca gatcatctat tgttacattc tctgttttgt
     2941 tatataccct ttgtactatt ggagacaaaa gaagaaagaa acaggaggaa agcttaagtt
     3001 gcttatgaag ttataatttt caattagctt ttggtgtcaa taagccactg tttttcaaaa
     3061 agaaaacatg acagacgaaa cgccctgatg tcagaaattt gagaaatatg gcagacgaaa
     3121 cgtccccttg tcaccttgtc acttgtcagg tcattttact tttatcttgt ctttgagctg
     3181 ttcgaatttt ttgtcgccta ttccggaaac gttttttatt tcttaataga tttaaatgga
     3241 ccgctagtgg tcctgtagtc tataattttt tgtgcggttg cagggcctat tcccggcaaa
     3301 gttgtaagtt cggtctcatt tgcagtattt atgtttactt taccgtcgtt tttcgagatg
     3361 atacccacag tagacccata gagctcatct tcctgctcag attgttcagg agattcccct
     3421 attttgggga catagagctt gtcttcatca tgtattttct gagcgagatt aagtcttaaa
     3481 agatcggcct cgggaagagg gccccctgct atttctatgg catcttttac ccgctggcct
     3541 tcttctaatg tatagacacc gggatttttt acagcgcctg ctatatggat gacaatcttc
     3601 tccgcctcat acgctggagc tgttaccgga actgccggtt catcctgaag tttaaatgct
     3661 atatctggtt ctttcttgaa aaaggcgtaa tatgatactg atactgtaga aatggtcaat
     3721 agcagtaaaa gacctaatag tattttttct cttttactga aatcaaacat ttctatcaca
     3781 ctccggctcc ctattctttt atccccatta ttctgtagag catatcaata tccaggctat
     3841 ttctcaccac atcagcccag cggtcatatt cagcttcttt atagttttta aaattaaaag
     3901 agtgagtttc aaaaatgata ctatcattta accttttact taccaaataa tttataaatt
     3961 gctgggtaaa atcagtattg tcaaaaattc catgtatgaa ggtaccaagt actcgacctg
     4021 tttgtgatat aagaccatca aagtcaacgt ggcctttgct cttgaacttt ataaactcta
     4081 tgtttccttc atcctcctta attatctctc cggagtaaat tctatagcct tccagctgtg
     4141 ccttgcttaa tatacttgca aaatcgggca aagaagaggc aacctctccc ttcactcgtg
     4201 taggctcctg cggcaataat ttaatatgta caccgaagag gcctaaaccc tcgatggttg
     4261 aatccgaaga agtagttttg aatgtcttgc caagcagtgc aaagctgctt cctatgccaa
     4321 ggaccaccgc acctttatga taaagcctgt taatatcttt atcccagccc cttgatttga
     4381 taaactctat actttcttta ggattttcag aaccgggaat tatcagcaaa tcagcatctt
     4441 ctattggccc taccgataca tatgagatct tgacaccctc aaactctgct aaaggcacaa
     4501 aatctgtaaa gttagtgatt cggggcaagc ttaaaatttt tacttgtaaa ggcttctgtt
     4561 ggagtgactc gttgctctcg accttatact ttttcaaaag cctatatctt tccgtctccg
     4621 gggaatcctc ttcatctatg taaatgtcgg tataggggac aactcctatg accggtcgct
     4681 ttattatgtc ttccagcatt tcaaggccgg gctctaggag ctttaaatct cctcgaaact
     4741 tgtttattat tactcccttc acccgtgctt tctcttcctc agagagcaaa agcagtgtcc
     4801 cggctagggc agcaaaaact ccgcccctat cgatatctcc taccaggaca acgggagcat
     4861 ctaccaattc cgccattccc atattcacta tgtctttttc cctcaggttt atctcggcgg
     4921 gactccctgc accttctatt actataatat cattgtctcg tgctagttca tcatagcact
     4981 ctttcacaac atgcaatagt ttaaccttgt aatcatggta atccattgcc gaaagactgc
     5041 caaaaggcct ccccttgact acaacctggc tgcttgtatc cgtatagggt tttaatagca
     5101 cgggattcat aaatacggaa ggctctatac cgcaggcctc tgcctgggta gcctgtgccc
     5161 tacccatttc cagaccctct ttagttatat atgaatttaa agccatattt tgagatttaa
     5221 atggtgcaac tttgaaacca tcctgcttga aaaccctaca aaggcccgta actagcctgc
     5281 tctttcccac agaagacccc gtgccctgca ccataataca tttggctgtc attgcaaaaa
     5341 acctcgaaga attacgtctt cagcttcttc ttcagtaaga cctaaggtca tcagctttaa
     5401 gagctgttcc tcggcaattc taccgattgc ggcttcgtgt gtcagctggg catcgggatg
     5461 ttcagctgtg atggcagggg tggaacttat tttcccctgg tccattaata tggaatcaca
     5521 ttctacatgg cccttacttt tctcataggc tatgattaca gggcggaata tctgcttgga
     5581 gttatctttg gctaccgacc tggatatgat ctgggcattg gaatctttcc cgacaagttc
     5641 gatggtcata tcagaaacag catactgact gccatcggta agcagtctct cggtgacaag
     5701 caggctggcc ctctctgcaa gcttggccct cgtgtccctt acggtgttat ctactccttt
     5761 aatctgaaca agctccattt caacaatgga gttttcttcc attgtaagca gggtcttggg
     5821 atttaggacc ttttcacctt ttccctctcc ctggccgtag tgcttttcta tataacgcat
     5881 ccgtgcaccc tttttcacta tgatatcatg gataccgtcg tgctgtgatt tttccgagcc
     5941 ggaattgtgt atcccgcaac cggctaccac ggtcacatcg gccccttcgc cgattattat
     6001 cgtgttgtat acaacatcct tgacgcctga catggttatc atgactggaa tgtgcacagc
     6061 ttcgtttttc gtgcccggag caactattat ttctatgccg ggtttgtccg attttggttt
     6121 aacttcaatg ttagctgaag agtggcgaat gataccttct ccattttttc tgatattaac
     6181 tgctccagta ggtattttgt gtaaatctgc gatttgcttt aatagttcag tttcaatagc
     6241 attcaatttc atcttcacta cctccacagt tttcttgcca gcagcatctt gaatcgggcc
     6301 gaatcattgg cagcatctta tcacaggggc ccctatccac aattttccca tcggccaaaa
     6361 gtattatttc atctgcaagg gataagaccc tttcatggtg cgttataact atgcttatta
     6421 cattgccttt ctcatgatgt tttcttatta gctgcagaag tttttcaaaa ctccaaagat
     6481 ccacacctgc ttccggctca tcatatatta caacttttga aggccttgaa agcacagtgg
     6541 caagctcaat tctcttcatc tcaccgccgg aaagactcga atcacattcc ctatcgagat
     6601 aatcctgggg acacaggcct actgctctaa gtcttgctct aagggagctt tcgtcggcgg
     6661 aaatccttaa tatgtctttt atctttatgc ccttaaatct aggaggagtt tggaaggcgt
     6721 aactgatgcc tgcctttgcc ctctcggtaa tgctcaggct tgttatatca gacccgttat
     6781 aaaatatttt tcctctatta ttcttataaa tgcccataat cactttggcc agcgaggttt
     6841 tgccgccgcc gtttgggccc gttagagcgt aaaacttgtt gtcttccaac tctaggttta
     6901 tgtcttttag tattg
//
LOCUS       DAONUO010000079         6866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8316_length_6866_cov_3.249743, whole genome shotgun sequence.
ACCESSION   DAONUO010000079 DAONUO010000000
VERSION     DAONUO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8316_length_6866_cov_3.249743"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK768_04155"
     CDS             <1..433
                     /locus_tag="PK768_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60192.1"
                     /translation="NLLKESIKDFMEEKVVQYERLLEEGMSFLEIVEEIVFDKSESVR
                     MMNYEFIQTILVKYQDMKQFIDDYYKNKAIPLMIRLIDKGRREGYINRDISNEAFLFY
                     IGIFKETINQYELISKLSKSAMQELSSLFFYGILGEKPSNL"
     gene            complement(580..1224)
                     /locus_tag="PK768_04160"
     CDS             complement(580..1224)
                     /locus_tag="PK768_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQA60193.1"
                     /translation="MKAIIFDMDGVIIDSMPLHHQIEREMLREYGYDISEEEHNTFAG
                     NTDYYMWSVMKERFNLEASVDELTAIKKELFVENLDKVKLLDNFYDFMLAVYNEKYLL
                     AMASSNTRKAIDKVMDIFSLSKYIKVSVSGEEVEKGKTNPEIFLKAAKKLGVEPKNCL
                     IIEDTFVGIQAAKAAGMKCIGFKNPNSGDQDLSGADLVVESFKELSVDTIKELL"
     gene            complement(1400..5008)
                     /locus_tag="PK768_04165"
     CDS             complement(1400..5008)
                     /locus_tag="PK768_04165"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HQA60194.1"
                     /translation="MGTKKLKLSENSKVVLKKRYLAKDEEGNVIETPEQLLERVSRAI
                     AKAEDKYSEGKSRYWQEKFYEEMAELKFLPNSPTLMNAGRELGQLSACFVLPVEDSME
                     GIFDSIKHAALIHKSGGGTGFSFSRLRPKGATVKSTGGVASGPISFMKVFNSATEAVK
                     QGGVRRGANMGILRVDHPDILEFIRCKQDDKEITNFNISVAITDEFMKALENDGEYDL
                     IDPHTNKVTGRLKAREVFDLIVDMAWNNGEPGIIYIDRMNEDNPTPSIGAIESTNPCV
                     TGDTLVATEEGLVRIDELYTRYKKGGFRIVTDNRVPTTQLMVANDGRLVEAVPNMGVS
                     GTTLNPVVQVYNNGKKEVMRLVTKSGFQIKATPDHRFLTTEGWKELREIKAGDEVLIQ
                     SGEGSFSKNDKLPFPVVNRYKGKNGRIYTFNFPQNWSRELGQVLGWLVGDGWLRDQGE
                     NYRVGFAFGKDDAEILDYLKGIINGMYGKDIREVKRINGVYHLSYHSKPMVNFFKDLG
                     VKSVGAQDKEVPEAIFKAPYYAVQGFLQGLFTADGTVRTNSKPNSEWIALTSKSKKLL
                     TQVQILLLNFGIKSVLMDRSRKPQEKKFRYVTIDGEERFYGTDGVLYELGVFGASRER
                     FRQKIGFIQSSKNQTLDNIVFDRFYAEKFYDEVELVEYVGEETVYDLTEPVTHSMICN
                     GLVVHQCGEQPLLPFESCNLGSINLAKFVKKDGDKSIIDYEGLRETVHTAVRLLDDVI
                     DVNLYPLKEIEEMTLANRKIGLGVMGFADLLIQLGIPYNSDEAVKMAEEVMSFIDSES
                     KKASEELAKERGVFPNWEKSIYKDKGMRLRNATTTTIAPTGTISIIAGASSGIEPIFA
                     LAFERHVLDNERLVEVHPLFKKALSDLGLYTDELMQEVIDKGSVQEIAGLPDKIRKVF
                     VTAHDITPEWHIKIQAAFQKYTDNAVSKTVNFPYSASREDVRDVYLMAYELGCKGVTI
                     YRDGSREEQVLNKGTKDKAKDETKDMTVAQRCEDCPIADMIPGSEIKPRPRPSVTYGN
                     TEKVKIGCGNLYITVNTDERGICEVFTNLGRAGGCPSQSEATSRLISMALRSGIDVKA
                     IVEQLKGIRCHSTLRQMANNKEIKVLSCPDAIGRAIERSIGLINGNGNNHESNYAYIK
                     RYGDTETDERAITKDESSSNVNKLLVKSSACPECGHTLEHDGGCVVCRSCGYSKCG"
     gene            complement(5314..5724)
                     /locus_tag="PK768_04170"
     CDS             complement(5314..5724)
                     /locus_tag="PK768_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQA60195.1"
                     /translation="MDTQKGFIRKTKQRDLILKVLRSTDTHPTADWIYEQVKKEMPNI
                     SLGTVYRNLGKLAELGEITELKFGSKYSRFDGNPDNHYHFVCTECEKVIDVKGCPVDE
                     EIDRCIELNNDVKVFFHKVLFYGLCTDCKNNEQE"
     gene            complement(5734..5859)
                     /locus_tag="PK768_04175"
     CDS             complement(5734..5859)
                     /locus_tag="PK768_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQA60196.1"
                     /translation="MAIFKCSECGYEREARCKPRKCPECEAKNTFTKVEAPQEDK"
     gene            complement(6302..>6866)
                     /locus_tag="PK768_04180"
     CDS             complement(6302..>6866)
                     /locus_tag="PK768_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQA60197.1"
                     /translation="EQFLEETFDKKGFNDLKVPLSVVSADINTGQTVVFCPRKNVPRK
                     KLSNTVFITDRSLATAVRASISLPGVFLPRVVKGHTLVDGGIKNNIPVDILHSQGATK
                     IIGVDLGVSESRAKVDSIIDILMATIDIMGDELSHYIRKEHPGYYIYPDIKGVGYKDF
                     HRIPEIVEYGEKVAERELPKIIKYLSS"
BASE COUNT         1812 a         1580 c         1201 g         2273 t
ORIGIN      
        1 aaacctccta aaggagtcta tcaaagactt tatggaggaa aaggtcgtac aatatgaacg
       61 cttactggaa gaaggaatgt ctttcctgga aatcgtcgag gaaatcgtct ttgacaaaag
      121 cgaatccgtt agaatgatga attacgaatt tattcaaacc attctagtaa aatatcagga
      181 tatgaagcaa tttatcgatg attattacaa gaataaagcc attcccctga tgatacggct
      241 aatagataaa ggccgcaggg aaggttatat aaatagggat atctcaaatg aagccttttt
      301 gttttacatt ggcattttta aagaaaccat aaatcaatac gagctaattt caaaactgtc
      361 caaatccgca atgcaggaat tatcctcgct tttcttttac ggcattttag gtgaaaaacc
      421 gtccaatttg tgagcagtat ttgacccggc atcgtttatg ggatggtttt ttgctttatg
      481 tgctatgaaa aaaaagacac cgggtttgtt tcggtgtctt ttcttcattt aaaaagtcga
      541 tgctgctccc taattagccg ggatatttaa gcatttcttt tatagaagct cttttatggt
      601 gtccacgctc aattccttaa agctctccac tactaaatca gcacccgaca aatcttgatc
      661 ccctgaattt ggatttttaa aacctataca tttcataccc gcagctttag ctgcctgtat
      721 accgacaaag gtatcttcga ttattaaaca attcttgggt tcgacaccca gcttttttgc
      781 agcttttaaa aatatttccg gattggtttt gcctttttct acttcttcac cggatacgga
      841 tacttttatg tacttggata gactaaatat atccataacc ttatctatgg cctttcgagt
      901 attagatgat gccattgcca gcaaatactt ttcattataa acagccagca taaagtcata
      961 gaaattatcc aacagcttga ctttgtccag gttttcgaca aataattcct tttttattgc
     1021 cgtcaattca tcaacagaag cttctaaatt gaatctttcc ttcatgacgc tccacatgta
     1081 ataatcggta tttcctgcaa aagtgttgtg ttcttcctca ctgatgtcat aaccgtattc
     1141 tctaagcatt tccctttcaa tttgatgatg taacggcata ctatcaatta ttacaccatc
     1201 catgtcaaaa ataatggctt tcaatttacc actccttgta ttagatctat gcattatttt
     1261 atcatagcag aaaattcgaa tattttcaat gagcctgtta actatcaaac gaaaaagctc
     1321 ccttaaagct aaactggtaa gccttaaggg agctcttcgt gtaaggatgg gattcctgtg
     1381 aaccattgat tgctctacat taaccacact tggagtagcc acagctgcgg catactacgc
     1441 agcctccatc gtgttcaagg gtatggccgc attcgggaca tgcagaactt tttacgagca
     1501 acttatttac atttgaactg ctttcatcct ttgttatagc cctttcatcg gtttcggtat
     1561 caccataacg cttaatatag gcgtagtttg attcatggtt gttgccgttt ccgtttatta
     1621 gacctatgct gcgctcaatg gccctgccta tggcatcggg acatgagagc acctttattt
     1681 ccttgttgtt ggccatctgg cgcaaggtag agtgacagcg tatgcccttt agctgctcga
     1741 ctatggcctt gacgtcaatc ccggaacgaa gggccatgga aatcaagcgg ctggtggcct
     1801 ctgactggga gggacagcct cctgcccggc caaggttggt aaacacttcg cagatgccac
     1861 gttcatcggt atttactgtg atgtacagat ttccgcagcc gatctttacc ttttcggtat
     1921 tgccgtaggt gacactgggc cttggccttg gctttatttc acttcccgga atcatgtcgg
     1981 ctatcgggca atcttcgcag cgttgagcta ccgtcatatc cttcgtctca tctttagcct
     2041 tatctttcgt gcctttattg aggacctgtt cctccctgct gccgtctctg tatatcgtca
     2101 cacctttgca gcccagttca taagccatca aataaacatc tcttacatct tccctgcttg
     2161 cactgtaggg gaagtttacg gtctttgata cggcattgtc ggtgtatttc tggaaggcag
     2221 cctggatctt tatatgccat tcgggagtaa tgtcatgggc agtgacaaag acctttctta
     2281 ttttgtccgg taaacccgct atctcctgaa ccgacccctt atctatgacc tcctgcatca
     2341 attcatcggt gtaaagccct aaatcgctta aagccttttt aaataagggg tgcacctcaa
     2401 ccaagcgctc attgtcaagc acatgcctct cgaaagccaa agcaaatatg ggctcgatgc
     2461 cgctggaagc tcccgctata atgcttatgg ttcccgtagg tgcaatagtt gtcgtggtgg
     2521 catttctcaa gcgcattccc ttgtctttgt aaatgctctt ttcccaattg gggaagaccc
     2581 ctcgctcttt tgccagttct tcggaggcct tttttgactc actgtcaata aagctcatta
     2641 cttcttcagc catttttacg gcttcatcgg aattataggg aatgccgagc tgtattaaca
     2701 gatccgcaaa gcccatcaca ccgagtccga tcttgcggtt ggccaaagtc atttcttcta
     2761 tttcctttaa aggatatagg tttacatcta tgacatcatc taataacctt acggcggtat
     2821 gcacggtttc cctcaggccc tcgtaatcga tgatgctctt atctccatcc tttttgacga
     2881 attttgcaag gtttatagaa cccaaattgc aactttcgaa tggtagcaga ggctgttctc
     2941 cgcattggtg aaccactagt ccattacata tcattgaatg ggtcaccggt tctgttaaat
     3001 cataaacagt ttcttctcct acatattcta ctaactcgac ttcgtcatag aatttttctg
     3061 cataaaacct gtcaaaaact atgttatcaa gagtttgatt tttgctcgac tgtataaatc
     3121 caatcttctg cctaaatctt tcccggctag ctccaaaaac acctagctcg tacaatactc
     3181 catcggtgcc ataaaatctt tcctctccgt cgatcgtcac atacctgaat tttttctcct
     3241 ggggctttcg agacctgtcc ataagcacac tttttatgcc gaaatttaaa agcaatattt
     3301 ggacctgggt taataatttt ttactctttg aggttagggc tatccactca ctattaggtt
     3361 tagaattggt ccttactgtg ccatcagctg taaataaacc ttgtaaaaag ccttgcactg
     3421 cgtaatacgg cgctttaaat atggcttccg gaacttcttt atcttgtgca ccaacgcttt
     3481 ttacaccaag gtccttgaaa aagttcacca taggtttgct atgataagat aaatgataaa
     3541 ctccatttat acgttttact tcacgtatat ccttgccgta cataccatta ataatacctt
     3601 taaggtaatc aagtatctct gcatcatcct taccgaaagc aaatcctacg cgatagtttt
     3661 caccctggtc tctgagccaa ccatctccta ctagccaacc taaaacttgt cccaattctc
     3721 gagaccaatt ttgtggaaaa ttaaatgtgt atatcctgcc atttttcccc ttatatctat
     3781 tgacaactgg aaaaggaagc ttgtcatttt tagaaaaact gccttcccct gattgaatta
     3841 atacttcgtc tcctgcttta atctctctaa gctctttcca accttcggtt gttaaaaatc
     3901 tatgatcagg agttgcttta atctgaaagc ctgattttgt cacaagacgc attacttctt
     3961 ttttaccatt attgtagacc tgcacaaccg ggtttaatgt tgttcccgaa acacccatat
     4021 taggtactgc ctcgacaagt cgtccatcat tggctaccat taattgggtt gttggaaccc
     4081 tgttgtcagt aactattcta aaaccgcctt ttttatatcg ggtataaagt tcatctattc
     4141 tgaccaagcc ttcttccgta gctaccaagg tgtcaccggt tacacaaggg ttggtgcttt
     4201 cgatggcccc gatggaaggg gtggggttgt cctcattcat cctatcgata tatatgattc
     4261 cgggctcgcc gttgttccag gccatgtcca caattaaatc gaaaacttcc ctagctttta
     4321 gcctacccgt aactttattg gtgtggggat ctatcaggtc gtattccccg tcattttcca
     4381 gggctttcat aaactcatct gtgattgcca cacttatgtt gaagttggtg atttccttat
     4441 cgtcctgctt gcacctaata aactctaaaa tatccggatg gtcaacccgc aaaataccca
     4501 tgttggcacc ccgacgaacc ccgccctgct ttacggcttc cgttgcagag ttgaatacct
     4561 tcatgaagga tatgggccct gaagccacgc cacctgtaga ctttaccgtg gcccctttgg
     4621 gacgaaggcg agaaaagcta aaacccgttc ctccaccgct tttatgtata agggctgcat
     4681 gctttatgga gtcaaaaatt ccctccatgg aatcttctac tggcagcaca aagcaagccg
     4741 ataactggcc aagctctcta ccggcattca taagtgtagg ggaattgggg agaaatttta
     4801 attccgccat ttcctcatag aacttttctt gccaatatct agacttgccc tcggagtact
     4861 tatcctccgc cttcgctata gctcgagata ccctttcaag caattgttcc ggtgtctcta
     4921 tcacattgcc ttcttcatct ttagccagat aacgcttctt caaaacgacc ttggagtttt
     4981 ccgataattt taactttttc gttcccattt atatcacctc atccgtttct gcctatttct
     5041 tcttgcattt tacccctttt ctctagtctc taacaagttt aaaattaaaa aaaagaatat
     5101 tcaatatact atatattgtg gtaatataat tatacaatac aacatgttga cttcaagggc
     5161 agccaagtcc tatatatgta catttttgtc tcttatttgc tgcgagctaa gtatttctct
     5221 gtattttatt accgcatata ttattatatg taaaacgcaa atataaaata gaaaaaggcg
     5281 atcttagttt tactagatcg ccatccttta aacttattcc tgctcgttgt ttttgcaatc
     5341 tgtgcaaagg ccataaaaca agactttgtg aaaaaacact ttcacatcat tattcagttc
     5401 aatgcatctg tcgatttctt cgtctacagg acatcctttt acgtcgatta ctttttcgca
     5461 ctcggtacat acaaaatgat aatggttgtc cggattgccg tcaaagcggc tgtatttgct
     5521 gccaaattta agttctgtaa tctcgcctaa ttcagccagc ttacctaaat ttctataaac
     5581 agtaccgaga ctgatatttg gcatttcttt ttttacctgt tcatatatcc agtctgctgt
     5641 gggatgagta tccgtgcttc gcaagacctt taaaatcaaa tccctctgct tggttttacg
     5701 aataaaacct ttttgcgtat ccatttataa aacctacttg tcttcctggg gtgcttctac
     5761 tttcgtaaat gtatttttgg cttcgcactc aggacatttt ctcggtttac aacgagcttc
     5821 tctttcgtat ccgcattcac tgcacttgaa aatagccatt gaggcacctc catattaata
     5881 atgattatta tttatatata gtatacctga aaaaatgaaa catgtcaatt tgcatcatca
     5941 gagtatcaat agtataattt ctcataattt tgtagcattt ttcgcaacta gtcacttttc
     6001 ctatctgcaa taggtcattc atagtattta ccgcgtgaaa aatttaatga aaaatttttt
     6061 ttattttttt cttgacattc ctgctttcat gtgtataata atattataat acttgtatac
     6121 aattatccaa aggaggttgc cccatggaaa aaaacattat ctattcaagc gagcaggaaa
     6181 acatttacga agattacatc ctggattatc tgtatcagtc agcatttatg gagcattaaa
     6241 gataaataat aaaggtccct ttactataag aggtgagtca ggcctctttt tattttgtac
     6301 ttcatgatga taaatattta ataatttttg gcagttccct ttcagccact ttctcaccgt
     6361 actctacgat ttccggtatc cggtgaaagt ccttatagcc aacacccttt atgtcgggat
     6421 atatataata tcccggatgt tcctttctta tataatgtga cagctcatct cccattatgt
     6481 caattgtagc cattaaaata tctattatag agtctacttt tgccctgctt tccgacaccc
     6541 ccaaatccac acctatgatt tttgtcgccc cttgggaatg cagtatatcc accgggatgt
     6601 tgttttttat gcctccatct accaaggtgt gacctttaac taccctggga agaaacacac
     6661 ccggcaagga aatgctcgcc ctaaccgctg tagccaaact cctgtcggtt ataaaaaccg
     6721 tgttgcttaa tttttttcgc ggaacatttt tccttgggca gaacaccact gtttgtcccg
     6781 tattaatatc cgcacttact acggataggg ggaccttcaa gtcattaaag ccttttttgt
     6841 caaatgtctc ttcaagaaac tgttcg
//
LOCUS       DAONUO010000080         6844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8348_length_6844_cov_4.032405, whole genome shotgun sequence.
ACCESSION   DAONUO010000080 DAONUO010000000
VERSION     DAONUO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8348_length_6844_cov_4.032405"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PK768_04185"
     CDS             complement(<1..1001)
                     /locus_tag="PK768_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA60198.1"
                     /translation="MFCIKRQRVVALLLLAVLVAASFISGCGQKTVQENQTANDDEQL
                     MTIRVAKQFGLVYAPLMVAEKIDFFSKYGLKVEWLTLGSGGAVREAMASNELDAAFMG
                     IPPYLIGWDKGLPAKIAAGYCVIPVSLVTYDENINTLEDFKAEHKIALPSPGSIQHIL
                     LSMALEKQLGDPNALDDNLVALPHPDGAQAMLAKKDVVAHFTTPPYLFEELEQPGYKV
                     VLDGFDAFGQDFNFNVALVTESYHDSNPLGYAAFVQGLNDAMNWVNENKEETAKLLAP
                     EFQIDEEKLLKYLTAEGVNYTTAPYGLMGFADFMQKAGYISKVPGSLSEIAWENILA"
     gene            1124..1576
                     /locus_tag="PK768_04190"
     CDS             1124..1576
                     /locus_tag="PK768_04190"
                     /inference="COORDINATES: protein motif:HMM:NF016194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HQA60199.1"
                     /translation="MKSYSLIFLIFVLGLLSKNRLVYISSGVLLLFSVLNLMPKSSSS
                     QKVFLDLGVILLVIGVMMPFTQNSISIQDLYKSLFAARGCISFVIGILSSVMAREGVE
                     LMKKSPEVMIGLLLGTIVGTAFFDGIPVGPLVAAGFAAFIINLFARIA"
     gene            1788..3104
                     /locus_tag="PK768_04195"
     CDS             1788..3104
                     /locus_tag="PK768_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HQA60200.1"
                     /translation="MKIKTIIMGAAGRDFHVFNTYFRDNELYEVVAFTATQIPNIEGR
                     KYPAILAGKLYPDGIPIYTEDQLPNLIKTHDVRQVVFAYSDISHEDVMHKASTVLAAG
                     ADFRLMGPKNTSLKSTKPVISVCAVRTGVGKSQTTRAVCKYFKEKGKKVVAIRHPMPY
                     GDLSKQICQRFATYEDLDLHQCTIEEREEYEPHIDSGIVVYAGVDYEVILREAEKEAD
                     VIIWDGGNNDFPFYHTDLSIVLVDPLRPGHELLYHPGETNLKLADLIIINKIDSANAA
                     DINTVRENIEKVNPKAQVIEAASPISVEEPEMVKGKRVLVIEDGPTLTHGEMGFGAGF
                     VAAKKFGAGTIVDAKPFAVGSIKETYEKYPHLNTILPAMGYGKEQIKELEQTINSIDC
                     DLVLSGTPIDITRVIKTNKPVIRVKYELQEIGQPDLKSVLAGFLQG"
     gene            complement(3284..4660)
                     /locus_tag="PK768_04200"
     CDS             complement(3284..4660)
                     /locus_tag="PK768_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQA60201.1"
                     /translation="MEKPNIKGLYEKILSDKKLKTIAISGLCCLVLLIALVSVYSRTV
                     LEVSVNDRVIGYVQNHEVLSEITEELKSRFEAKLGADVEFVQVIKATPVRAVGHKLAV
                     EDEIIKDLEAALTCKLKAVAINIDGKEIALVKDKGTAENVLQQVKQHYIDQTPGELIK
                     LEVAENVKLVEKYVYPNQIISPEDAKNLILKGSVETRTYEVVEGDSLWAISRRENIPI
                     EELIKANPQLKSEHELALGDIINLTEVKPLLTVSMLKKVTYEESIPYKTETVRDDSMW
                     TWEQKVKQAGEKGTKEVAAEVLFENGIKVSENVIGEKVLKEPVTQIVAKGTKAEVAFR
                     GSGRFIWPVVGQITSPYGKRGREFHTGIDIAQSRGTPVYASNSGTVTFAGWRGGYGNL
                     VIVNHGGGIETYYAHNSKINVSVGQQVEKGQQIAAVGSTGRSSGNHLHFEIRINGSPV
                     NPLNYLNK"
     gene            complement(4958..6463)
                     /locus_tag="PK768_04205"
     CDS             complement(4958..6463)
                     /locus_tag="PK768_04205"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQA60202.1"
                     /translation="MKKRRIVIILSLILLAAAYSFFVLRSESDVDTGKTADIIANTCP
                     RYKIDVDFDPEAKKLTAFQEVAIVNSKAEEFESLYFHIYPNAFKNADEVPFADEDISY
                     AYPEGFAPGYINIKKVTAGTREVEFNIEDTILEIKLETALKPKEEISLTFEFEVQIPP
                     AIGNFGYGENTYNIAQWYPILAVYDEKGWHKDPYYGIGDPFYSEVAQYEVKIKAPKEY
                     TIAASGSLDEKHEENGRVAWSFKTGLIRDFAWSASASFETREATVGKSKVISYYIKGK
                     EEYGQKALEFGKNALSFFNEYFGEYPYENYSIVAADFIFSGMEYPNLVIVNEEFYEQG
                     MYLDYLEYTIVHETAHQWWYGLVGNNQIEEAWLDEALTEYSTVLYYEHYYGKAVAQDI
                     YERFTLAPYRYFETSMSLGPIKRHLSEFANWDDYSATVYNRGAIMLKDIEARMGKEKL
                     REALKFYFEQNIYDNATAEDFISALNRVTGTDWTDYVNEWLETTEILESAA"
BASE COUNT         1804 a         1633 c         1398 g         2009 t
ORIGIN      
        1 tgtgctaaga tgttctccca ggctatttca gaaaggctcc cgggcacctt ggatatatag
       61 ccggcctttt gcataaagtc tgcaaagccc atcaggccgt acggagccgt tgtgtagttt
      121 actccctcag cagtcaggta ttttagtagc ttttcttcgt caatttgaaa ttccggtgca
      181 agaagctttg ccgtctcctc cttgttttcg tttacccaat tcattgcatc atttaatccc
      241 tgtacaaagg ctgcataacc taaggggttg ctgtcgtggt aagactcggt taccaatgcc
      301 acgttaaagt taaaatcctg gccaaaggca tcaaagccgt cgaggacaac tttgtagccg
      361 ggctgttcca gctcttcgaa gaggtacggc ggtgtggtga aatgggcaac tacatccttt
      421 ttggccagca ttgcctgggc cccatccgga tgcggcagcg ctactaaatt gtcatccaag
      481 gcatttggat ctcccaattg tttttccagg gccatggata gcagtatatg ctggatgctg
      541 cccggtgacg gcagtgctat tttatgttcc gccttgaaat cttccagagt gttgatgttc
      601 tcatcatagg tcaccagcga cacgggaatt acgcaatatc ctgcggcaat tttggcaggt
      661 agtcccttat cccatcctat gaggtacggc ggaattccca taaaggctgc atccagttca
      721 ttggatgcca tagcttccct cacggctccg ccggagccca gggtcaacca ctcgactttt
      781 agcccgtact tggagaaaaa gtcaattttc tccgccacca taaggggagc gtaaaccaga
      841 ccgaattgtt tagctacgcg aatcgtcata agctgttcat catcattcgc tgtctgattt
      901 tcctggacag ttttctgtcc gcatcctgaa ataaagctcg ccgcaacgag gacagccagc
      961 agcagtaaag caaccaccct ttgtcttttg atacaaaaca tcccatcacc ctccaaaaaa
     1021 gtaaatctca taccataata ttttttggga cgggaatttg tgaattttat ttagttcatg
     1081 caaataaagt tttattaagt aaagactttt tggaggtggc gctttgaaat cctattcgtt
     1141 gattttccta attttcgtcc tgggcctgtt gagcaagaat cggcttgttt acatatcctc
     1201 aggtgtgctt ttgctgtttt ccgtacttaa tctcatgccg aaatcttcct catcccagaa
     1261 agtcttcctg gatttgggag tcatcctgct agtaataggg gtaatgatgc cctttaccca
     1321 gaactccatt tctatacagg acctttacaa gagccttttt gccgcaaggg gctgtatatc
     1381 ctttgtcata ggcatcctct cttcagtaat ggcaagagag ggagttgaac tcatgaaaaa
     1441 aagcccagaa gtgatgatag ggctgcttct tggcacaatt gtcggcactg ccttttttga
     1501 cggcataccg gttgggccgc tagttgccgc aggttttgcc gcatttatta taaacttgtt
     1561 cgcaaggatt gcatgaggtc atgtagccgg cattggtccc gacatgagcg tataaatact
     1621 tatctagtaa aagccagtat tagcagattt tcattttgat acaatagact ttgaaatttt
     1681 ttctgtgaat atttatgcat atatattgca tttttataag ataggtgtat aatattttta
     1741 taagtttaat cgatcagaaa ttcacaaaaa gcattaggag gattcttatg aagataaaaa
     1801 ccataatcat gggtgctgca ggaagagatt ttcatgtgtt caacacttat tttagagaca
     1861 acgagctcta tgaagtggtg gcctttacgg caacgcaaat tccaaacatc gaaggcagga
     1921 agtaccccgc gatacttgca ggcaagttgt accccgacgg aatacccatt tacacggaag
     1981 accagctacc aaatttgata aaaacacacg atgtcaggca ggttgttttt gcctacagtg
     2041 acatctccca tgaagatgtg atgcacaagg cttcaactgt ccttgctgcc ggtgccgatt
     2101 ttcgcctgat gggtccaaaa aacacttcat taaaatccac aaaacccgta atttccgtat
     2161 gtgccgtaag gaccggcgtg ggcaagagcc aaaccacccg cgccgtgtgc aagtacttta
     2221 aggaaaaggg caagaaagta gtggccatac gacatcccat gccctatggg gatttgagca
     2281 agcaaatatg tcagcgcttt gctacatatg aagatttgga cttgcaccag tgcaccatcg
     2341 aagagcgaga ggagtacgaa ccccacatcg acagcggcat cgtggtttat gcaggtgtgg
     2401 attatgaagt gattttaagg gaagcggaaa aagaggccga tgtaatcata tgggacgggg
     2461 ggaacaatga ctttcccttc tatcacaccg acctatccat agtcctcgtt gacccccttc
     2521 ggccgggaca cgaactcctg tatcaccccg gagaaaccaa tttaaaacta gctgacctta
     2581 taataattaa taaaatagat tcggccaatg ctgccgacat aaataccgtc agggaaaaca
     2641 tcgaaaaggt aaatccgaag gctcaggtta tagaggcagc ttcgcccatc agcgtagaag
     2701 agcccgagat ggtaaagggc aagcgggtcc tggtcatcga agacggtccc acactcaccc
     2761 acggagaaat gggattcggt gcaggctttg tggctgccaa aaagtttggc gccggcacga
     2821 ttgtggatgc caagcccttt gcggtaggaa gcataaaaga aacatatgaa aaatatcctc
     2881 atttaaatac cattttgccg gccatgggct acggcaaaga gcagataaaa gaactggaac
     2941 agaccataaa cagcatcgac tgtgacctgg tcctctccgg cactcccatt gatataaccc
     3001 gcgtcattaa gacaaacaag cccgtaatca gggttaagta cgagctgcag gagatcggtc
     3061 agccggacct caaatcggtg ctggcggggt ttttacaagg ctagccaacg cttaacccaa
     3121 gcattggaag aatgatttgc aagaggggta aacaccccct ccgacgaaca attgaagatt
     3181 gctaacggag caactaatat tattgttttt caacagcaaa aaggggctgc cttgagcaac
     3241 ccctttatcg tttcataatt tatttttatt tatacatgct gagctacttg ttcaggtagt
     3301 tcagcggatt tacagggctt ccgttgattc gaatttcaaa gtggagatga ttgccgctgg
     3361 acctgcccgt cgagccgaca gcagctatct gctggccctt ttctacctgc tggccaaccg
     3421 aaacgttgat cttgctgttg tgggcataat aagtttcaat gccaccgccg tgatttacga
     3481 ttaccaggtt gccgtaacca ccccgccagc cggcaaaggt tacggtaccg ctgttagagg
     3541 cgtaaacggg tgtacccctg ctttgggcaa tatcgatacc tgtatgaaac tccctgcctc
     3601 tcttgccata gggcgatgtg atttgtccaa caaccggcca tatgaatctt ccgctgccgc
     3661 ggaaagctac ttccgccttg gtccccttag ccactatctg ggttacgggc tccttaagca
     3721 ctttttcgcc tatgacattt tccgatacct ttatgccgtt ttcaaacaac acttcagcgg
     3781 caacttcctt ggtacccttt tcgcccgctt gctttacctt ttgctcccat gtccacatgg
     3841 aatcatctct gaccgtttcg gttttgtacg gaattgactc ctcatatgtc acctttttca
     3901 acatggaaac ggtaagtagc ggtttcactt cggttaggtt tattatatct cctaaggcta
     3961 actcatgttc ggactttagc tggggattgg cctttatcaa ttcctcaatg ggaatattct
     4021 cccttcggga aatagcccag agactatcac cttcaactac ctcataagtc ctggtttcaa
     4081 cgctgccttt gagaatgaga ttcttggcgt cttcaggact tataatttgg tttggataaa
     4141 catatttttc taccagctta acattttccg caacttcaag ttttatcaat tctccgggcg
     4201 tctggtcaat gtaatgctgc ttaacctgtt gcaacacatt ttccgccgtc cccttgtctt
     4261 ttaccagcgc aatttctttt ccatctatgt taatggctac tgctttaagc ttgcaggtca
     4321 acgcagcttc aaggtcttta attatctcat cttcaacagc cagtttatgg ccaacggccc
     4381 tcacgggtgt ggcctttatt acctggacga actcaacatc tgcgccgagt tttgcttcaa
     4441 acctgctctt aagctcttcc gtaatttccg aaagcacttc gtgattttgt acatagccga
     4501 taaccctatc gttaacacta acttccaaga ccgttcggct gtagacagat accagcgcga
     4561 ttaaaagtac gaggcaacaa agccccgaga ttgctatggt cttaagcttt ttatcgctca
     4621 gtattttctc gtacagcccc tttatattag gcttttccat ccccccactc cttccttttt
     4681 tgtccgcatt ttctagcctc aaagccttct cgcaaagggc atttaatatg atgctaattt
     4741 tttgtaatca aattgtaaca tactatatta tattctatgc aaatcctgtt tttccttcct
     4801 agatttaaaa tattgtccag actatcacta tgtaaattct ggaaggccct caaaaacccg
     4861 ttaagataat aattcccata attgccataa atatacctgg atagcttttc caaatcccgc
     4921 aaaaaaagga ggactaaaga cctataaggt aatttaatta tgctgcgctt tccagtattt
     4981 cagttgtttc aagccactca ttcacgtaat cggtccagtc agtcccggta acccggttta
     5041 aggcacttat gaaatcttca gccgtggcat tatcataaat attttgctca aaatagaatt
     5101 tcagggcttc acggagcttt tcctttccca tgcgtgcttc tatatctttt agcataatgg
     5161 cgcccctgtt atacaccgta gcactgtaat catcccagtt cgcaaactcc gacagatgcc
     5221 ttttgatggg gccaagagac attgatgtct caaaatacct atacggggcc aacgtgaacc
     5281 tttcgtatat gtcttgcgcc accgctttgc cgtaatagtg ttcataatat agaacggtag
     5341 aatactccgt caaagcctca tcaagccatg cttcctctat ttgattgttt cctacaaggc
     5401 cataccacca ctgatgggca gtctcgtgaa ctatcgtata ctccaggtaa tctaaataca
     5461 tcccctgctc gtagaattct tcatttacta ttacaaggtt ggggtattcc atgccgctaa
     5521 agataaagtc cgcagccact atgctataat tctcataggg atattcccca aaatattcat
     5581 taaagaaaga taatgcattc ttgccgaatt caagggcttt ctgaccgtat tcttccttgc
     5641 cctttatgta gtaagatatg actttgcttt ttcccacggt tgcctctcta gtttcaaaac
     5701 tagcacttgc tgaccaggca aagtcccgaa tcaagccggt cttaaagctc caggctaccc
     5761 ggccgttttc ttcgtgcttt tcatccaggc tcccgctggc agcgatggtg tattccttcg
     5821 gagctttgat ttttacttca tactgggcca cctcgctgta aaaggggtcg cctatgccgt
     5881 aataaggatc cttatgccag cccttttcat cgtacacggc gagtatcggg taccattggg
     5941 caatgttgta ggtgttttca ccgtatccaa aattgccaat ggccggaggg atttggactt
     6001 caaattcaaa agtcaaactg atttcttctt ttggttttag tgccgtttcc aattttattt
     6061 ccaaaatcgt atcctcaata ttaaattcca cttcccttgt ccctgcagtt acttttttaa
     6121 tatttatgta gccaggagca aagccttcag gatatgcata gctgatatct tcgtcggcaa
     6181 agggcacttc atcggcgttc ttgaaagcat ttgggtagat gtgaaagtat agcgactcaa
     6241 attcttcggc cttgctgttt acaattgcca cctcctgaaa tgcggtaagc tttttagctt
     6301 cagggtcaaa atccacatct atcttatacc taggacatgt attagcaatg atatctgcag
     6361 ttttacctgt atccacatcg ctttccgacc taagaacaaa aaaactataa gcagctgcaa
     6421 gcagtatgag actcaaaatg ataacaatcc ttcgcttctt cattttcttg cctccctttc
     6481 acatcttctt tccctcttat tgtaccatct ttaattaata tatttaatag ttttgattat
     6541 tatcatttta ttcgtttttt atttgtaaat cttatctgtt gatacgatag atataaaaaa
     6601 taaaacgaca gtgggccatt aaaaaaacac taactacatc caattggtta aatattctag
     6661 gctataaaaa cggataaact catttcctct gcttccggct gcactgcaag taaaacccgg
     6721 cattgatgac aggccctgat ttgcaaggca tataaaaaaa agaaaggttt caagctttta
     6781 aaaagcctga aacccttttt tgcaatatgg agcggaaaac gggatttgaa cccgcgaccc
     6841 tcgg
//
LOCUS       DAONUO010000081         6792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8442_length_6792_cov_3.824996, whole genome shotgun sequence.
ACCESSION   DAONUO010000081 DAONUO010000000
VERSION     DAONUO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8442_length_6792_cov_3.824996"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PK768_04210"
     CDS             <1..764
                     /locus_tag="PK768_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HQA60203.1"
                     /translation="RGHGHCIAKGGKLPQMFAELLGKETGYCRGRGGSMHIADLALGN
                     LGANGIVGGGIPIATGAALASKLKNDGKVTLCFFGDGATSQGAFHEAVNLASVKKLPV
                     VYVCENNLYGMSVPFSKASAVENAADRAKAYNIPGIIVDGNDVEAVYGVVKEAVERAR
                     KGEGPSLIETKTYRWLGHSKSDPRVYRTREEEEEWKQRCPIKRYRQQLISEGVDEEEL
                     TKIEKEVEGAIQEALEYAQNSPEPKVEEIMDDVYA"
     gene            790..1767
                     /locus_tag="PK768_04215"
     CDS             790..1767
                     /locus_tag="PK768_04215"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HQA60204.1"
                     /translation="MAEKLYIEALREGLREEMIRDENVFIIGEDVGLYGGAFGVTKGL
                     FEEFGENRIIDTPISEAAIAGAAVGAALCGMRPVAEIMFFDFFTIAMDQLVNQGAKNR
                     YMFGGKAKVPMVIRGPMGCGTGAAAQHSQSFPSVLAHFPGLKLVMPATPYDVKGLIKS
                     AIRDDNPVVFAEHKLLYWTKGEVPEGDYTVPIGKADVKRQGKDITIVAGSIMVPRSLE
                     AAQKLEEEGIDVEVMDIRSLKPLDLQTVVSSVKKTGRVLIVEDDPISYGWGAEVAAGI
                     VGSEAFDYLDAPVKRLAGLDIPIPYNPNLEKHAVPQVPNIIDAVKEVLR"
     gene            1780..3087
                     /locus_tag="PK768_04220"
     CDS             1780..3087
                     /locus_tag="PK768_04220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQA60205.1"
                     /translation="MATIVKMPKLGTTMTEGTIVKWLKNEGDPVEKGEIYVEIQTDKV
                     NIEDEAEASGILRKIIVKEGETVKVGEPIAIIAEADEELPDLEVVEDVADGKQEESAS
                     VLTQEEDKIAEAIEAVPGKIKASPAAKRVAREKQIDLSDVTPSGPGGRIVERDVLAYI
                     QEHKVRATPVAKKIAIKEQIDLGSLQKAPGERITKEDLEERLIEQEIVDTIPVTGMRK
                     IIADSMAYSKQVAPHIYLSLEVDMSKVIDLRERLIPLVQSHRDVKLSYNHILIKAVAT
                     ALKLNPLINSSFTEEKIILKQNINIGLAVALEDGLIVPVIKDADKKGLAVIAKETQEL
                     IQKAKTKKLLPDDYQGGTFTITNLGMFDIENFCAIINQPETAILAVGKIIKKPVVVDD
                     EITIRPMMNLTLSCDHRAIDGAVGAKFLQNVKQILEEPMNMLL"
     gene            3101..4483
                     /gene="lpdA"
                     /locus_tag="PK768_04225"
     CDS             3101..4483
                     /gene="lpdA"
                     /locus_tag="PK768_04225"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQA60206.1"
                     /translation="MHYDIAIIGGGPGGYVAAIYAGKKKAKVALVEKGELGGTCLNRG
                     CIPTKSLIHGANLLQAIKSAKDFGITADNVNIDWDKLQKKTSRIVKTLTKGVENLLKA
                     NNVTVMQGRAKLSRKNTIEISGETGQSTITADNIILATGSVPTLVPIPGKELQNVMTS
                     DQALFLGEIPSSILIIGGGVIGLEIGYVYNSFGCRITVVEMLPEILPRQDKEISLELK
                     KQLERQGIEIYTDSMVRKIREKDGILQTVIETKEGTKTVDSEKILMATGRAPAVDAFK
                     DIGLHIEKSGVVVDEYLKTNIDNIYAVGDVTGKSMLAHVASHQGITAVKNILGENEKI
                     DYKAIPSCVYTSPEVASVGMTEDEARRLFRDNIRVGRFPYAASGKAKTLGEVNGFVKI
                     ISESKYNEILGVHIVGPSATELIAEAVLAIKLECTAEELANTIHAHPTLSEATMEAAF
                     DLLAEPIHKL"
     gene            4500..4841
                     /locus_tag="PK768_04230"
     CDS             4500..4841
                     /locus_tag="PK768_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQA60207.1"
                     /translation="MTVMTGWQAVVQAIKSEGIRFVYGLPSSPEDLYDALYDEPSIKA
                     IQVRHEAAGGFMAMAHAYRCYGERVEKPDDIRGALERALKANESGKPAILDIIIALDD
                     VAEGFIEYKKL"
     gene            5262..6644
                     /locus_tag="PK768_04235"
     CDS             5262..6644
                     /locus_tag="PK768_04235"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQA60208.1"
                     /translation="MAENVEKTMNKIVSLCKRRGFIFQGSEIYGGLANTWDYGPLGAE
                     LKRNVKDYWWKKVVHERDDVVGLDSAILMHPRTWQASGHLEGFNDALIDCKQCKSRFR
                     ADHLIEDSLNMKVEGKSVQELTEIIHGNEIKCPTCGMVAWTEARVFNLMFKTFQGVTE
                     DSSSIVYLRPETAQGIFVNFKNVLDSSRMKLPFGIAQIGKSFRNEITPGNFIFRTREF
                     EQMELEFFVKPGEDEKWFEYWRQFMMDFFVSLGVKKENLRFRDHSPEELSHYSKATTD
                     LEYKFPFGWGELWGTANRTDYDLSRHIEYSGKTLTYFDPEENKHIVPYVVEPSLGADR
                     AILVLLCDAYDEEEVQGETRVVLRISSVIAPIKCAILPLSRKEPLEELARKIYGDLRK
                     HYHAEYDNIGSIGRRYRRQDEIGTPFAITVDFDSLEDNKVTIRDRDTMKQDRVEIGNI
                     RDYLKGKLGF"
BASE COUNT         2185 a         1269 c         1724 g         1614 t
ORIGIN      
        1 accgaggcca cggacactgc attgctaaag gcggcaaact gccccaaatg tttgcagagc
       61 tgctgggaaa agaaactggt tactgcagag gaagaggagg gtcaatgcat atcgctgacc
      121 tggcattggg caatttaggt gccaacggca ttgtgggtgg aggcataccc attgccacag
      181 gggctgcctt ggcgtcaaaa ctcaaaaatg atggaaaggt aactttgtgc ttttttggag
      241 acggagccac aagtcaaggg gcttttcatg aggctgtaaa cctggcttca gttaaaaaat
      301 tgccggtggt atatgtatgt gaaaacaatc tttacggcat gtcagttcct tttagcaagg
      361 ccagtgctgt tgagaatgca gcagatagag caaaagctta caatatcccc ggcattatag
      421 tagacggcaa tgatgtggaa gccgtgtacg gagtggtaaa agaagctgtg gagagggcaa
      481 ggaaagggga aggcccatct ctaatagaga ccaaaaccta tcgctggctt ggccattcaa
      541 aaagcgatcc aagggtttac cgcacgagag aggaagaaga agagtggaaa cagagatgtc
      601 ctataaagag gtataggcaa caacttattt ctgaaggggt agatgaagag gaacttacaa
      661 agattgaaaa agaagtagaa ggggcaattc aagaggccct agagtatgcc caaaacagtc
      721 ctgaacctaa agtagaagaa attatggacg atgtatatgc ataagaaaaa actgcaggag
      781 gcgataataa tggctgaaaa gctttatata gaggcgttaa gagaaggctt aagagaagaa
      841 atgattcgag acgaaaacgt cttcatcatc ggtgaagacg tggggcttta cggcggtgcc
      901 tttggagtga ctaagggcct ttttgaggaa tttggggaaa ataggataat agatactccc
      961 atctctgaag cggccatagc cggtgctgct gtgggagcgg ccctatgcgg aatgcggccc
     1021 gttgcagaaa taatgttttt cgactttttc accattgcca tggaccaact tgtaaaccaa
     1081 ggggcaaaaa acaggtacat gtttggtgga aaggccaagg tacccatggt aattcgaggg
     1141 ccaatgggat gtggaaccgg agctgcggcc cagcactctc aaagctttcc gtcagtgcta
     1201 gcgcattttc cgggcctaaa gttggttatg ccggcgacgc cttacgatgt caaagggcta
     1261 ataaaatccg ctatcaggga tgacaatcct gtggtttttg cagaacacaa gctattatac
     1321 tggactaaag gcgaggttcc tgaaggagac tacaccgtgc ccatagggaa agccgatgta
     1381 aagaggcagg ggaaagatat aaccatagtt gccggttcca ttatggtgcc aagatctctt
     1441 gaggcggccc aaaagcttga ggaagagggc atagacgttg aagttatgga tattaggtct
     1501 ttaaagccgc tggacttaca aacagtggta tcttctgtca agaagacggg aagagtgcta
     1561 atcgtggaag atgatcctat cagctacggt tggggagcgg aagttgctgc aggcattgtg
     1621 ggcagtgaag cttttgatta tctggatgct ccagtaaaga gacttgccgg tcttgatatt
     1681 ccgataccct ataatccgaa tcttgaaaaa catgccgtac cgcaagttcc caacatcata
     1741 gatgccgtaa aggaagtact taggtaaagg aggatagtta tggcaacgat agtgaaaatg
     1801 cctaagctgg gcacgaccat gaccgaaggc actatagtta aatggctgaa gaatgagggc
     1861 gaccccgtag aaaaaggtga aatatatgtg gaaatacaga cggacaaggt aaatatcgaa
     1921 gatgaggctg aagcctcagg gattttgagg aagataatcg taaaagaagg tgaaacggtt
     1981 aaagttggtg agcccattgc cataatagcc gaagccgatg aggaactgcc ggatttggaa
     2041 gtagttgagg atgtagcaga tgggaaacaa gaggaatcgg cttcagtttt gacgcaggag
     2101 gaagacaaga tagcagaagc aatagaggcg gtcccgggaa aaattaaggc atctcctgca
     2161 gctaagcggg ttgcacggga aaagcagata gacctttccg atgttactcc ttctggcccg
     2221 ggcggcagga tagtggaaag ggatgtacta gcctatatac aggaacacaa ggtgagagct
     2281 acacctgtcg ctaagaaaat tgcaattaag gagcaaatag acctggggtc attacaaaaa
     2341 gctcccggtg agagaataac aaaggaagac ctggaagaac gtctaattga acaagaaata
     2401 gtagatacta ttcccgttac cggcatgagg aaaatcattg cagacagcat ggcctacagc
     2461 aaacaagtgg cacctcacat atatctctcc ttggaagttg acatgtcaaa ggtcatcgac
     2521 ctaagggaaa gactaatacc ccttgtacaa tcacatcgtg atgtaaaact gtcatataac
     2581 cacatattga ttaaggcagt tgctacggcc ttaaagctta atcccttgat caattcaagt
     2641 tttacagaag aaaagatcat tctcaaacag aatattaaca ttggacttgc agtagcctta
     2701 gaagacggcc taattgtccc cgttataaag gatgcggata aaaagggcct tgcagttatt
     2761 gcaaaagaaa cacaggaact gattcaaaaa gcaaaaacca aaaagctgct gcctgatgac
     2821 tatcagggcg gcacatttac gataacaaac cttggaatgt ttgacataga aaacttctgt
     2881 gcaataataa atcagcctga gaccgctata cttgctgttg gtaagataat taaaaaacct
     2941 gttgttgtag atgatgagat aacaattaga cccatgatga atctgacctt atcctgtgac
     3001 cacagggcaa tagacggtgc tgttggagca aaatttttgc aaaatgtcaa gcaaatcctt
     3061 gaagaaccga tgaacatgtt gctttaaagg aagtgatgaa atgcattacg atatagccat
     3121 tatcggcgga gggcccggtg gctatgtggc tgccatttat gcgggcaaaa agaaggccaa
     3181 ggtggccctt gttgaaaagg gagagcttgg tgggacctgt ctcaacagag gctgcattcc
     3241 tacaaaatca ttaatacatg gtgcaaactt gctgcaagcc ataaaaagtg ccaaggattt
     3301 cgggattacg gcagacaatg taaatatcga ttgggataaa ttgcagaaaa agacatcccg
     3361 gatagtaaag accctgacaa agggtgtgga aaacctgcta aaggctaata atgtgacggt
     3421 aatgcagggc agagcgaaac tttcccgcaa aaacaccata gaaatatcgg gtgaaaccgg
     3481 tcaaagcacc attactgcag ataacataat cctggccacc ggctccgttc ctactttagt
     3541 gccaattccc ggtaaagagc ttcaaaatgt catgacaagt gaccaggccc ttttcttagg
     3601 tgagattcca tcctcaattc ttattatcgg cggtggagtc ataggtcttg agatcggata
     3661 tgtatacaat agctttggct gcagaataac cgtagtggaa atgctgccgg aaatccttcc
     3721 aaggcaggat aaagaaatat ctttagaact taaaaaacag ctggaaagac aaggcattga
     3781 aatatacact gattcaatgg tcagaaaaat aagggaaaaa gatggcattc tccaaacagt
     3841 aatcgagaca aaagaaggca cgaaaaccgt agattcggaa aagattctaa tggcaactgg
     3901 gagagccccg gcggttgatg cttttaagga cataggcctt catatagaaa aaagcggcgt
     3961 tgttgtggat gagtatctta agacgaatat agacaacatc tacgccgttg gtgatgtgac
     4021 cggcaaatca atgctggccc atgttgcatc acaccaggga ataaccgccg ttaaaaacat
     4081 actgggggaa aatgagaaaa ttgactacaa agctatacct tcctgcgtct atacgagtcc
     4141 cgaagtagca tccgttggaa tgacggaaga cgaggcaaga aggttgttta gagacaatat
     4201 cagggtgggt agatttccgt atgccgccag cggcaaggca aagactttag gagaggtaaa
     4261 cggatttgta aagattatat ccgaatcaaa gtacaacgaa atattgggag tccacatcgt
     4321 gggaccaagt gcaacggaac ttattgccga agctgtcctt gcgataaagc ttgaatgcac
     4381 ggcagaagaa ctggcaaata ccattcatgc ccatcccaca ctctcggaag caactatgga
     4441 agcggccttt gacctgctgg cagagcccat acacaagttg taaggagagg tgtgaaaata
     4501 tgaccgtaat gactgggtgg caggcagtgg ttcaggctat aaaatccgag ggcatcagat
     4561 ttgtctacgg gcttcccagt agccccgaag acttatacga tgcgctgtac gatgagccgt
     4621 ctattaaagc aatacaggta aggcatgaag cagccggggg tttcatggca atggcccatg
     4681 cttaccggtg ctatggggaa agagtcgaaa aacccgacga tattcgcggc gccttagagc
     4741 gggccttgaa agcaaatgaa tctggaaaac cggctattct cgatatcata atagctcttg
     4801 atgatgtagc cgaaggtttt atagaatata aaaagctata aaacggtcat atccgaaata
     4861 tatcatcaat aagactttat ccatattcac caataaaatg ttgctaaatt gtattccatg
     4921 ttttttataa aaatgttaca aatactgcct aattcttgtc atacaccttg acttaagctt
     4981 aacaatagta tatattttac atagaaactt tctggtgatc ttttttccct gctttaagcg
     5041 agcggaggat ggtgcgagct ccgtagcata aaaaaggtct gggaacagaa agtcatcagc
     5101 caaaacaaag aataatctga tcccaaaagt ggcaggtaag catctatggc ttgcttgcaa
     5161 ctagggtgga accgtccgtg aaaaaggacc cctagaccga tgttaaggtt taggggtttt
     5221 ttaatattaa aaacaaatta atttggagga ggtaaatgat tatggctgaa aatgttgaaa
     5281 aaaccatgaa caagatagtt tcactttgta agcggagggg ctttattttt cagggatctg
     5341 aaatatacgg aggtcttgcc aatacttggg attatggtcc tttgggagct gaacttaaga
     5401 gaaacgttaa ggattattgg tggaaaaagg tggtccatga aagagacgat gtagttggtc
     5461 ttgacagtgc aatattgatg caccccagga catggcaggc ttccggtcac ttggagggct
     5521 ttaacgacgc cttgatagac tgtaagcagt gcaaatccag attcagggct gaccatctta
     5581 ttgaggattc actcaatatg aaggtagaag gtaaaagcgt tcaggaactg acggagatta
     5641 ttcatgggaa tgaaataaaa tgcccaacct gcggcatggt tgcctggaca gaggctagag
     5701 ttttcaacct gatgtttaaa accttccaag gggttaccga ggattcaagc tcaatagtat
     5761 atctacgacc agaaacggca cagggtatat ttgtcaactt taaaaacgta cttgactcca
     5821 gtaggatgaa acttcctttt ggcatagccc aaataggcaa atccttcaga aatgagatta
     5881 cgccggggaa cttcatcttt agaacccgag aatttgagca aatggaatta gaattttttg
     5941 tgaaaccggg tgaagatgaa aagtggtttg aatattggcg gcagtttatg atggatttct
     6001 tcgtatcttt aggtgttaaa aaagagaatc tgcggtttag agaccattca cctgaagagc
     6061 tgtctcacta ttctaaagct actacagact tagaatataa gtttcctttt ggatggggcg
     6121 agctttgggg aactgcgaat cgaacagatt acgatctctc gagacacatc gagtactctg
     6181 ggaagacatt aacctatttc gatccggagg aaaacaaaca cattgtgccc tatgtggtag
     6241 aaccatccct gggtgctgac agggctatat tagttctgtt gtgtgatgcg tatgacgaag
     6301 aagaggtcca gggtgagaca agggtggtcc tgcgcatttc gtcggtaatt gcgccgataa
     6361 aatgtgcgat tcttcccctt tccagaaaag aacccttgga agaactggcc agaaagatat
     6421 acggtgactt gagaaaacac taccatgctg aatatgacaa catcggcagc ataggcagac
     6481 gctaccgacg tcaagatgaa atcgggacgc cttttgctat taccgtagac tttgacagct
     6541 tggaagataa caaagtcacc ataagagata gggataccat gaagcaagat agggttgaaa
     6601 ttggcaatat aagggattat ttgaaaggga agctagggtt ttaagtatta gaacataggc
     6661 tgctatagga tgatttttcc tgtagcagct tttttctaga tgggaaaagc tcaagtagca
     6721 gatttttaaa cgctttgaaa acttaacatt gcaacaagtt gacatagtgg caataaaccg
     6781 tatacaaaaa tt
//
LOCUS       DAONUO010000082         6776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8472_length_6776_cov_3.489064, whole genome shotgun sequence.
ACCESSION   DAONUO010000082 DAONUO010000000
VERSION     DAONUO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8472_length_6776_cov_3.489064"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK768_04240"
     CDS             <1..182
                     /locus_tag="PK768_04240"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777820.1"
                     /note="catalyzes the formation of D-fructose
                     1,6-bisphosphate from D-fructose 6-phosphate in
                     glycolysis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HQA60209.1"
                     /translation="RILASRFGGKAIDLLKENVRDKMIGLINGEMVLTDIDKVLSTTK
                     PLDMEVHELADILSY"
     gene            203..1612
                     /gene="pyk"
                     /locus_tag="PK768_04245"
     CDS             203..1612
                     /gene="pyk"
                     /locus_tag="PK768_04245"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQA60210.1"
                     /translation="MLKTKIVCTLGPSSENKYVIKQLIIAGMNIARINLSHGNHEEHR
                     KRINALRKACEELNANVAVLLDTKGPEIRLGTFYGGKVLLDKGQEFVLTPTPMIGNER
                     EVFINYDHIAETVKPGEKILLADGLIELCVKDIQGDKVVCTVVNGGELSDRQGVNIPN
                     VSLSLPALTQKDLEDILFAIDIGADFIAASFVRKAEDVMEIRRVLQENGGSDIHIIAK
                     IENQEGLQNIDEIIEVSDGIMIARGDLGVEIPVQEVPVVQKQIIAKCNAAGKPVITAT
                     QMLDSMIRNPRPTRAEATDVANAIFDGTDAIMLSGETAAGKYPVEAVTMMASIAKKAD
                     EVVFNDVEKRHITVKSITDAISHATCSIASELGAKAIITSTKSGYTARAVAKFRPKAP
                     IIAVTFNEKVIKTLQLIRGVIPLKVKQTFSTDEMFREAINGALNSGIVISGDLVVITA
                     GVPINISGTTNLIRVHVVE"
     gene            1824..3869
                     /locus_tag="PK768_04250"
     CDS             1824..3869
                     /locus_tag="PK768_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQA60211.1"
                     /translation="MKKKAIQIIFSVFLLFFILSSVSLVFAGPKQLRVAVNTNMAPYQ
                     FIGEDGECVGLHIDILNWIAQRKGLELFYVPYEKSHECLKALSNGEVDIVLGHKSYDT
                     VGYEFQLTGELSRSSICMVAPKELVNELNERADYKSLSAALEYGTLDYTHMNNLGLRK
                     YVSYGNQQMVVDALISGEVDIAFGIKECFDYLFAQKGIADNYTILRNYLSPISYSMLV
                     REDDEELFTDLDTGLTELRTSGKYESIYRNWIVDRDLIKAQQRQQLITRLLGIAAVGT
                     SLALLVMAFNVRMNILLKRKVDEKTKELRDANTQLERMVEQLHSEGRLRNSIIEDSPN
                     AMIFIDSTYCITLANSAARNMAGVEELVGKNIKSVGVFSEILGQIRYDIFSAQEDSVD
                     KPMIVEIGQDETKQSYRCIFYRSSDYSTIEGMLMTVENVTAEELKKQERFEAEKNKAL
                     NRLVAGIAHEIKNPLMAIKTACTLILNQGDDPEIKNAFADFVPYEIERINQLVEGLIS
                     YARPTKDKTELFDLSKLVNDCLYLTSIAAKRSRICLETEIEDNLYISAKKVQIKQSLI
                     NIIINGIESMEKKLAGSAKPDGSIKMKITAYKNDKNAIVVVRDEGVGMTAEEIKQCTK
                     PFFSTKPAGTGLGLAIVKQYIQENNGILHIKSEKNKYTEITLTFRRYDLSEKQDSNH"
     gene            3844..5214
                     /locus_tag="PK768_04255"
     CDS             3844..5214
                     /locus_tag="PK768_04255"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQA60212.1"
                     /translation="MRNKILIIDDELSTCTMLKLALRGKYEVKYATDAIEGLSMLKAE
                     RFDLVLLDLIIGEHDGIEILKEIKDYDGDVAVIMITAFGNIKSSVEAIKNGAFNYLSK
                     PIDMDELFVFIEQALRVKNLSAEILYLSDELKNHRFQDHGIIGKSAAIRKVYTMIEKL
                     KDVDTTVTITGESGTGKELVARAIHFTGKRKDKRFVVINCAAIPENLLEGELFGYKRG
                     TFTGAMQDKKGKFEVADKGTVFLDEIGDMQLNLQSKLLRVLQYKEYTPLGSNEVHKTD
                     VRIIAATNKDLEVLVKEGSFREDLFYRLNVVNIHVPPLRERKEDIPLLCQHFIEKFGR
                     EQNKRINKITPDAMEALMSYDYPGNIRQLANFIEHAMIISNGDTIDIKDLPGTVRGTL
                     HYEQEESYEKKFRERLSRMSIKEIERIAIEATLEKNDGKRGPTAKDLGISIRSLHNKI
                     KEYGLV"
     gene            5411..6469
                     /locus_tag="PK768_04260"
     CDS             5411..6469
                     /locus_tag="PK768_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HQA60213.1"
                     /translation="MKAGRIQFTYLSQSDLLKAGCFDIEMALNAAEKGLVAYHNNEVL
                     FPEKIVQIFNEETQERINCLPATFIKDKICGVKWVSVFPMNPVKYGTQNLSAVIILSE
                     IERGYPVAVMDGTLCSNIRVAAMGGLAAKYLARKDSEIIGFIGAGEQAKMHLLAMKHV
                     LPKLRECRVCAKTAHEEEQFIEELSPLIPGMEFVSTKTDGKKAMEGADVLVTATSAQA
                     PLLKAGWMKLGSFYSHIGGWEDEYAVAKQCDKIVCDDWETVKHRTQTISRMFKEGLLK
                     DEDIYANIVDIVIGKKKGRESDAERIYFNAVGLSYIDIAIAMAMFARARAANIGQILD
                     LQERMIFENKQLIQYVKL"
BASE COUNT         2230 a         1180 c         1600 g         1766 t
ORIGIN      
        1 acaggatatt ggccagtcgc ttcggaggta aggctatcga cctgttaaaa gagaatgtca
       61 gagacaagat gatcggctta atcaatggcg agatggtttt aaccgacata gataaggtac
      121 tttcaactac caagccctta gacatggaag tgcatgagct agccgacata ctgtcatatt
      181 aaagcatgag gatgtgatga ccatgctgaa aaccaaaatc gtatgcaccc ttggcccttc
      241 cagtgaaaac aagtacgtaa taaagcagct gattatagca ggcatgaata ttgcgcgcat
      301 taacttatcc cacgggaatc atgaagagca caggaaaaga atcaatgccc taagaaaggc
      361 ctgcgaagaa ttaaatgcca atgttgcagt attgctggat accaaggggc cggaaataag
      421 gctgggaact ttttacggtg gaaaggtgtt gcttgacaaa ggtcaggaat ttgtcctcac
      481 tcccaccccc atgatcggca atgagagaga agttttcatc aattacgatc atatcgcaga
      541 gactgtaaag cctggcgaaa aaatactttt ggcagatggc ctaattgagc tttgcgtaaa
      601 agacatacaa ggggataagg ttgtatgcac cgttgtaaac ggcggcgaat tgagcgatag
      661 acagggagta aacataccca atgtgtcgct ttcattgccg gcattgactc aaaaggacct
      721 ggaagatatt ctgtttgcca tcgatatagg tgccgatttc atcgcggcgt cttttgtcag
      781 aaaagctgag gatgttatgg aaataaggcg agtgctgcag gaaaacggcg gcagcgacat
      841 acatataatt gctaaaatcg aaaaccagga aggtctgcaa aatatcgatg aaataataga
      901 agtttcggat ggcatcatga tagcacgggg cgacttgggt gtcgaaattc cggttcaaga
      961 agtgccggta gtccaaaaac agattattgc caaatgcaac gctgcaggaa aaccggtaat
     1021 cacagctacc caaatgctgg attccatgat tagaaatcca agacccacta gggctgaagc
     1081 cactgacgtg gccaatgcta tctttgatgg caccgacgct attatgcttt caggagaaac
     1141 cgctgctggc aaataccccg tggaagctgt gacgatgatg gccagcatcg caaaaaaagc
     1201 cgatgaagtg gtctttaatg atgtcgaaaa aaggcacatc accgtaaaat ccataaccga
     1261 tgcgataagc catgcaacat gctcaatcgc atcagaactt ggggcaaagg caataataac
     1321 ctcaaccaag tccggataca cggcaagagc agttgccaag ttcaggccaa aggcccccat
     1381 aattgcagta accttcaatg aaaaggtcat taagaccctg caactcatac gcggcgtaat
     1441 acctctgaaa gtaaaacaaa cattctccac tgacgaaatg tttcgcgaag ctataaatgg
     1501 agcattaaac tccggtatag taataagcgg cgacttggtc gtaataactg ctggagtgcc
     1561 aattaatata tccgggacaa ccaacctgat tcgcgtccac gtggtagaat aatttatgac
     1621 gagatatatt tttccttagt agaattagat acaaggtaca aaagaactta taaacgggct
     1681 gtctttttgt cttttaatat tttttttaaa aataattttc ccaaaaatcc cgaaattagc
     1741 aatatgacta aagttctgcg gtttctttac tcttcatgtt tttatgtgcg gattcggtca
     1801 gccaatccgg aggcagggat atcatgaaaa aaaaggccat acaaatcata tttagtgttt
     1861 ttctgctttt ttttatattg tcatctgtat ctcttgtttt cgcaggaccc aaacaactac
     1921 gagttgcggt taatactaat atggctcctt atcagtttat aggagaagac ggggaatgcg
     1981 tggggttaca tatagatatc ctgaactgga ttgctcaaag gaaaggactg gaactttttt
     2041 atgttcctta tgaaaaaagc cacgaatgcc taaaggccct tagcaatggg gaggtagata
     2101 tagttcttgg acacaaatcc tatgatacgg ttgggtatga gtttcaactt accggagagc
     2161 tctcccggtc ttctatatgt atggttgctc ccaaagagct ggtaaacgag cttaacgaac
     2221 gggccgatta taaatcactg tcggcagccc tcgagtacgg tactttggat tatactcaca
     2281 tgaataatct tggattaagg aaatatgtgt catacggcaa ccagcaaatg gttgttgatg
     2341 cgctcatatc aggtgaagta gacattgctt tcggcattaa agaatgcttc gattacctgt
     2401 ttgctcaaaa aggcattgcg gacaattata ctatacttcg caactacctt tctcctatca
     2461 gttattcaat gttagttaga gaagatgatg aagagctatt cacggattta gatacgggtc
     2521 ttaccgaact gcgcaccagt ggtaaatacg aaagcattta ccgcaattgg atcgttgacc
     2581 gtgaccttat aaaagctcaa cagcggcaac aattgattac aaggctgttg ggcattgctg
     2641 cggttggaac ttctctagct ttactggtta tggcatttaa tgtaaggatg aatattttac
     2701 ttaaacgcaa agttgatgaa aaaacaaaag aattgagaga tgcgaataca cagcttgaac
     2761 gcatggtaga acaattgcat agtgaaggac gcttgagaaa tagcattatt gaggattctc
     2821 ccaatgctat gatttttatt gacagcactt attgcattac attagcaaac agtgcagccc
     2881 gtaacatggc tggagtagaa gaactggttg gtaaaaacat caagagtgtg ggggtattta
     2941 gtgagatact aggacagatt agatatgaca ttttttctgc gcaggaagat agcgtggaca
     3001 aaccaatgat tgtagaaatt gggcaggatg aaacaaagca aagttatcgc tgtatatttt
     3061 atcgttccag tgattacagc actattgaag gcatgctgat gaccgtggag aatgttactg
     3121 ccgaggagtt gaagaagcag gagcgatttg aggcagaaaa gaataaagct ctaaacaggc
     3181 ttgtggctgg aatagcacac gagataaaaa atcctttaat ggctataaaa actgcttgta
     3241 cgcttattct aaatcaaggt gacgaccctg aaataaaaaa cgcatttgca gattttgtac
     3301 cctacgagat tgaaagaatc aaccagcttg ttgagggcct tataagttat gcacgtccta
     3361 ctaaggataa gaccgagctg tttgaccttt cgaagctcgt aaatgattgt ctatatttaa
     3421 ccagcattgc ggctaagagg agtagaatct gtcttgaaac cgagattgaa gacaatttat
     3481 atatttcagc taaaaaggtc caaataaagc aatcgttgat caatattatt atcaacggta
     3541 tagagtcgat ggagaaaaaa ttggccgggt ctgcaaaacc ggacggcagc attaagatga
     3601 agataactgc ttataaaaac gacaaaaatg cgatcgttgt tgtccgtgat gagggagtag
     3661 gcatgactgc cgaagaaatc aagcaatgta caaagccgtt tttttcgaca aaacctgcag
     3721 gcacaggtct gggactggct attgttaagc aatatattca agaaaacaat gggatcttac
     3781 atattaaaag cgaaaagaac aaatatacgg aaattacctt gacattccgg aggtatgacc
     3841 taagtgagaa acaagattct aatcattgac gatgaattaa gtacatgcac tatgctaaaa
     3901 ctggcattgc gtggcaaata cgaggtaaaa tatgcaacag atgcgataga ggggctctcg
     3961 atgcttaagg cagagcgatt tgatctagtc ttattagacc ttataattgg tgaacatgat
     4021 ggtattgaga ttcttaaaga gattaaggat tatgacggcg atgtagctgt gataatgatt
     4081 accgcttttg gcaatatcaa gtcatcggta gaagcgataa aaaatggagc ctttaattat
     4141 ctttcaaaac ccattgacat ggacgaactg tttgttttca tagagcaagc attgagggtc
     4201 aagaatctta gcgccgaaat actttattta tcggacgaac tcaagaacca ccgctttcag
     4261 gaccacggaa taattggcaa aagtgctgct attagaaagg tttacacgat gattgaaaag
     4321 ctaaaggacg ttgatactac cgtgacaatc accggagaaa gcggaacagg caaggaactg
     4381 gttgcccgtg ccattcattt tactggaaag cgtaaagata aaagatttgt cgttatcaat
     4441 tgcgctgcta ttccggaaaa cctgttagag ggagaactct ttggttataa acggggcacc
     4501 tttacaggag caatgcagga taaaaagggc aaatttgagg ttgcggacaa gggaacggtt
     4561 ttcctggatg aaatcggtga catgcagctt aacctccaga gcaaattgtt gagagtgctg
     4621 cagtataagg aatatactcc attaggaagt aacgaggtac ataagactga tgtgcggatt
     4681 attgcagcca ccaacaaaga tttggaggtc ctggttaaag aaggcagttt tcgggaagac
     4741 ctgttttatc ggttaaacgt agtgaatatt catgtacctc cgctgagaga gcgcaaggag
     4801 gatattccac tattgtgcca gcattttatt gagaaatttg gacgtgaaca gaataagcgc
     4861 attaataaga taacaccgga tgctatggag gctctcatgt catacgatta cccgggaaat
     4921 atcaggcagc ttgctaattt tatagagcat gccatgataa tatccaacgg tgatactatc
     4981 gatataaaag acctgccggg aacagtaaga gggactttgc attacgaaca agaggagtct
     5041 tatgagaaaa aattccgtga gcgtttatca agaatgtcta tcaaggagat tgagcggata
     5101 gccattgagg caacacttga aaagaatgac ggaaaacggg gccctactgc taaagactta
     5161 ggtatcagta ttagaagttt gcataataag ataaaagaat acggcttggt ctaagtttta
     5221 atcctgtatt gttacagaag ctataggcaa attctgcagt ctaacatgca gatattgcct
     5281 atagcttttt tatttttggc gtaaacaaga gtagcatctg gggccaaact cagcgtgaga
     5341 gttggcacat gaattgcttg aattatgtac aggaatataa aactatttaa gggagggaaa
     5401 agttgacaca atgaaagcag gaagaattca gtttacatat ttatctcaaa gtgacttgtt
     5461 gaaggctgga tgtttcgata tcgaaatggc gttaaatgcg gcagaaaagg gattagtagc
     5521 gtatcacaat aacgaagttt tatttcctga aaagattgtg caaatcttta acgaagagac
     5581 acaggaacgc ataaactgtt tgcccgctac ctttataaaa gataaaatct gtggtgtaaa
     5641 atgggtttct gtgtttccga tgaatccggt aaaatatggt acccaaaact tgtcagctgt
     5701 tatcattttg tcggaaatag aaagaggata tccagttgca gttatggatg ggacattatg
     5761 ctctaacata agggttgcgg caatgggagg actggcagca aagtacttgg caagaaagga
     5821 ttcagaaata ataggtttta ttggtgccgg agagcaggct aaaatgcact tattggccat
     5881 gaaacacgtt cttcccaagc tgcgggaatg tcgcgtttgt gcaaaaactg cccatgaaga
     5941 agagcagttc attgaagaat taagtccgtt gataccggga atggagtttg tgtcaaccaa
     6001 aaccgatggg aaaaaagcta tggagggagc agatgtttta gttactgcga caagtgctca
     6061 ggcgccttta ttgaaagctg gatggatgaa gctgggttct ttttacagcc acataggtgg
     6121 ctgggaagat gagtatgcgg tagcaaagca gtgtgacaag attgtgtgtg acgattggga
     6181 aacagtcaaa caccgtaccc agactataag taggatgttt aaagaggggc tacttaagga
     6241 cgaggatata tacgcaaata tagtcgacat agttattggc aagaaaaagg gtagggaaag
     6301 cgatgctgaa aggatttact tcaatgcggt cggcttgtca tatatagata ttgccattgc
     6361 catggctatg tttgctagag cccgtgcggc gaatattgga caaatactgg atttacaaga
     6421 aagaatgatt tttgagaaca aacaactaat tcaatacgta aaattataac attaatggca
     6481 aaagtttgaa ccgtcggcaa atattaccgg tcgaagtgca aaatttgccg atgatttttg
     6541 attgtgagaa tgaaaggaaa agaaaaagtt gcatagaaaa caggaaaaaa gctgttcaat
     6601 ggagttggca tacaaattgc taaaatgatt atatacagaa aatacagaaa ctattaatta
     6661 aaagggagga ttaaagatga acaaaacaaa aaagaatata gctctattgc tattagtttg
     6721 ccttgtgatg actgttttaa gcggttgcgg tcaatcgact tcgaaacagt ctgagg
//
LOCUS       DAONUO010000083         6722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8543_length_6722_cov_4.369132, whole genome shotgun sequence.
ACCESSION   DAONUO010000083 DAONUO010000000
VERSION     DAONUO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8543_length_6722_cov_4.369132"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..701
                     /locus_tag="PK768_04265"
     CDS             15..701
                     /locus_tag="PK768_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQA60214.1"
                     /translation="MAQFENQDVHTTTSAATSTLQQLGQLEVPKMESAIHTITIVGQI
                     EGHLVLPPQNKTTKYEHIIPQLVAIEQNPDIKGLLIILNTVGGDVEAGLAIAEMIVSM
                     TKPTVSLVLGGGHSIGVPIAVASNYSFIAETATMTIHPIRLTGLVIGVPQTYEYLDKM
                     QDRVIKFVIEHSNIDEDTFRELMFSTGKLARDIGTVLVGSDAVKCGLIDEVGGIGQAV
                     SKLEELINRG"
     gene            703..930
                     /locus_tag="PK768_04270"
     CDS             703..930
                     /locus_tag="PK768_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HQA60215.1"
                     /translation="MILLILYTSMPIELVIEGMDKKYEFKEIEIEAVKLIIEPISVNQ
                     GKIVKLISTDPQDYLNPNLSPGKIITFALNK"
     gene            1007..3181
                     /locus_tag="PK768_04275"
     CDS             1007..3181
                     /locus_tag="PK768_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQA60216.1"
                     /translation="MAKTTRNIKNDIRWEIKGLLFISFGVLGIFSIYTNAVGVIGKFI
                     SKNLKGFSGQAAVLIPLIIILLGVNCLFYKKKLKISLRVIGLVIIFAVIVFLFHINTH
                     ANLREMNFLDRLRKSAELGVNGQGGGILGESGLSLLISIFGTTGSTIMMITFFIIGSI
                     LATGKSITTILERIKLSTKRKKITINPQIGSKKIKVVNNIPQDSSSIDEVDDNSETAT
                     RNTSSTQKEEVQKIKSVKNEEDSRDSIIITDYIEDDKEYNLPPLSLLRKNSGKQRGFC
                     EKELLDNAQTLENTLKSFGLEAKVIQVNCGPTITRFEIQPSPGIKVSRIMSLADDIAL
                     SLAASDVRIEAPIPGKAAVGIEVPNKKKSLVLMRDVLESTDFVKSPSKLSIALGMDID
                     GNPKVADLTEMPHLLIAGATGSGKSVCIKTIISSILFKAHPNEVKLMMIDPKVVELAI
                     FDGIPHLLTPVVTDAKKAAAALSWMVGEMERRYQLFAEEGVRDIARYNELYNDKPMPK
                     IVVIIDELADLMMVAPRDVEDCICRLAQMARAAGIHLVVATQRPSVDVITGLIKANIP
                     SRISFAVSSQVDSRTILDISGAEKLLGKGDMLFYPVGAPKPVRIQGAYITEEEVEKLI
                     NYCKKQKEPAYEKNLSDFNEVGVDKSHEETDELFEEAVKVVLDSGQASISMLQRRLRI
                     GYARAARLIDEMEQRGIVGGYEGTKPREILISKEHYEKYFQN"
     gene            3260..4114
                     /locus_tag="PK768_04280"
     CDS             3260..4114
                     /locus_tag="PK768_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQA60217.1"
                     /translation="MSLDLDGIKDLTQLGQYLKNIREERNISIEEIHKETKIRKRYLT
                     AIEEGNFSAIPGGDVYVRGFLRNYSISVGIQASAVIELYDKLKSTHKEEKNLPMFSNT
                     DSVQIDVFSSKFYSFFERNYKRVAAIILTGLLLVVLIMSIRALIDKSIQGNMESQPET
                     PTTQATAPELQDDYQVENIPIDDNKSVMVEIVEDTAHNTIFIIDDEQIEVTMDDIIDR
                     CWISVQKDGQFDFEGILNPGDAKTWEAKDAINMRIGNPPAVNLIVNGNDLGRPRAGAR
                     DFIFKKRT"
     gene            4230..5552
                     /gene="rimO"
                     /locus_tag="PK768_04285"
     CDS             4230..5552
                     /gene="rimO"
                     /locus_tag="PK768_04285"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQA60218.1"
                     /translation="MSAKIGFISLGCDKNLVDSEYMLGILKKHNYKITNDEEEAEIII
                     INTCGFINDAKQESIDTILETAQLKKTGKLKHLIAAGCLAQRYKSELLREIPELDAIV
                     GTGDFDKIHEVIEQLDSKRIELTDNKSFLDYDIGNRIRVYPYISFVKIAEGCDNCCSY
                     CAIPLIKGHYKSRKISTIKEEVENLVAEGVKEINLVAQDTTNYGVDIYGKPSLPKLLE
                     ELVKIREDFIIRILYAYPTNINKELLDVIKNNNKIAKYLDIPLQHINDRILKLMGRPS
                     SSKFIMQLIDDIRKQIPEITLRTTFIVGFPTETETEFNQLLDFVKLYRFDKVGAFQYS
                     KEEGTFAAALNPEVPDKIKYRRYSELMAVQQSISKTKNQRFKGKVLKVLVEKYDKENR
                     LFIGRSENDAPFVDGVIKILDGNCELGQFHKVKIIDSQEYDLLGELAN"
     gene            5657..6187
                     /locus_tag="PK768_04290"
     CDS             5657..6187
                     /locus_tag="PK768_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60219.1"
                     /translation="MKKLLDLDKTKILRILAISSFFLFLMLSLQWYFGKNVKLRALER
                     ELLEHKYVSSVEINEQKEQVVVCLSLRNIDNFKEAYDKICETIKERLKMQPFELKIIN
                     EADPELEDIFDNKVQFIVYEALATGEFTKMRASLDEIENTSDSLRVQVFLDSSKLFLH
                     IRLKESNYYRIINREV"
     gene            6214..>6722
                     /locus_tag="PK768_04295"
     CDS             6214..>6722
                     /locus_tag="PK768_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA60220.1"
                     /translation="MLLEIGIGLGIASIIYLAINGINVLPVIIMFGFSYMLYSIMSSN
                     NLIKSPQKLITNEQGITFDQIGGQEVAKNELIEALNFIKNFDEAKKMGIRPLKGILLT
                     GAPGTGKTLMAKAATSYIDSVFVSASGSEFIEMYAGVGAQRVRQLFETARTMARKASK
                     KHGVVFIDE"
BASE COUNT         2373 a         1094 c         1402 g         1853 t
ORIGIN      
        1 ggaaggatga tcaaatggca caatttgaaa accaagatgt gcacacgacc acaagcgctg
       61 ctacgagtac attacagcag ctaggccaat tggaagtgcc taaaatggaa agcgctattc
      121 acaccattac tatcgtgggt caaattgaag gtcatttggt attgcctccg cagaataaaa
      181 ctacgaaata cgaacatatt attccgcaac tagttgcaat cgaacaaaat cctgatatca
      241 aaggtttgct aatcatattg aacactgtag gaggagacgt agaggcaggc cttgccattg
      301 cagaaatgat tgtcagcatg acaaagccta cagtttcctt ggtgcttgga ggtggtcaca
      361 gcatcggagt tcccattgcc gtggccagca actattcatt tattgcggaa acggcgacca
      421 tgacaattca tccaattcgg ctcactgggc tcgtaattgg cgtacctcaa acatatgaat
      481 atctcgataa aatgcaagat agggtaatta aatttgtcat agagcattcc aatattgacg
      541 aggatacttt tagagaacta atgttcagta caggtaaact agctagggat atcgggactg
      601 tgctggttgg ctcggatgca gtaaaatgcg gtttaatcga cgaagttggt ggtatagggc
      661 aagcagttag caaactagaa gaattgatta atcgaggata aggtgattct attgatactt
      721 tatacttcaa tgcctataga actggtaatt gagggaatgg ataaaaaata cgagtttaaa
      781 gaaatagaaa ttgaagcagt gaagctgatt attgaaccaa taagtgtgaa ccaggggaag
      841 atagttaaac tcatcagcac agatccacaa gattatttaa atcctaacct ttcacctgga
      901 aaaataataa cctttgcgtt aaacaaataa ggccaaaagg ccttttttta ttgatcagaa
      961 aggattttat taataaatgg agaataaaac taatgaggtg taggatatgg caaaaacaac
     1021 tcgcaatata aaaaatgaca ttcgctggga aataaaaggc cttcttttta ttagttttgg
     1081 agtcctggga atattctcaa tttatacaaa tgcagtcggt gtaataggaa aattcataag
     1141 caagaatctt aaaggttttt ctgggcaagc agcggtatta atacccttga ttataatttt
     1201 gcttggtgta aattgccttt tctacaaaaa aaagctaaag atttccttga gggtaatagg
     1261 ccttgtaatt attttcgctg taattgtatt tttatttcat ataaataccc atgcaaattt
     1321 acgagaaatg aactttttag atagattgag aaaaagtgct gaactaggcg tcaatggaca
     1381 aggcgggggt attttaggtg aaagcggtct gtcgttgctg ataagcatat ttggcaccac
     1441 aggttcaact ataatgatga ttacattttt tattattggc tcaatccttg ctaccggcaa
     1501 atcaataaca acaatactgg aaagaataaa actatcaact aaaaggaaaa aaataactat
     1561 aaatcctcaa atagggagta agaaaataaa agtagtaaac aatattcctc aagacagttc
     1621 ttcaattgac gaagttgatg ataacagtga aacagcaaca agaaatacga gttcgacaca
     1681 aaaggaagaa gtgcaaaaga taaagtcggt gaaaaatgag gaagactctc gtgactcaat
     1741 cattattact gactacatcg aagatgataa agaatataac cttccaccat tatcattact
     1801 tcgcaaaaat tctggaaaac aaaggggttt ttgtgaaaaa gagctattag ataatgcaca
     1861 gactcttgaa aacactttaa aaagctttgg tcttgaagcg aaagtcattc aggtaaattg
     1921 cggtcctacc attacaaggt ttgaaataca gccatcaccc ggtattaaag tgagtaggat
     1981 tatgagctta gctgatgaca ttgctttaag tttagcagca tctgatgtta gaatagaagc
     2041 ccccattcct ggcaaggctg cagttggcat agaagtgcca aacaaaaaga aatccttagt
     2101 gcttatgcgg gatgtacttg aatctacaga ttttgtcaaa tctccatcga agctcagcat
     2161 agccttaggt atggatattg acggaaatcc aaaggtggca gaccttaccg agatgcccca
     2221 ccttttaatt gctggagcta cgggttcagg aaaaagcgtt tgcattaaaa ccattatttc
     2281 aagtatctta ttcaaggcac atcccaatga agtaaaactc atgatgatag atccgaaagt
     2341 tgtggaactt gccatatttg acggcattcc ccatctactt actcccgtag ttaccgacgc
     2401 aaaaaaagct gctgcagctt taagttggat ggttggtgaa atggagagga gataccaatt
     2461 atttgcggag gaaggagtca gagacatagc caggtataac gaattgtata acgacaagcc
     2521 aatgccgaaa atagtagtca ttatagatga attggcagat ctaatgatgg tcgcgcccag
     2581 agacgttgaa gactgtatat gcagactggc tcaaatggca agggcagcag gcatacactt
     2641 ggtggttgct acccaaagac cttcggtaga tgtaataacc ggtctaatta aagccaatat
     2701 cccttccagg atttcctttg cagtttcctc acaggtggat tcaaggacga tcctagatat
     2761 atctggggct gaaaagttac tgggtaaagg tgatatgctc ttctatccgg taggggctcc
     2821 taaacccgtt agaattcaag gagcctatat aaccgaagag gaagtggaaa aacttataaa
     2881 ttattgcaaa aaacaaaaag aacctgctta tgaaaaaaac ctttccgatt ttaatgaagt
     2941 tggagttgat aaatcccatg aagaaacaga tgagttgttc gaagaggccg taaaagtggt
     3001 gctggacagc gggcaagctt ctatttcaat gcttcaacgt cgacttagaa taggatatgc
     3061 tagagctgca agactcatag acgaaatgga acaacgtggt attgtaggtg gatacgaagg
     3121 caccaaaccc agagaaatat tgattagtaa ggaacattat gaaaaatatt tccaaaactg
     3181 atattttata gatactatga aaaaccgttt gtattagatg tgaattcata tagtaatata
     3241 agaacggggg gatggtaaat tgagcttgga tcttgatggg ataaaagact tgactcaact
     3301 tggccagtat ctgaaaaata tcagggaaga gcgaaacata tctattgagg aaatacacaa
     3361 ggagacaaaa attcgaaaaa gatatttgac tgctattgaa gagggcaact tttctgcaat
     3421 acccggcggt gatgtatacg tcagaggttt tcttaggaat tattctatct ctgttggcat
     3481 acaagcttct gccgttattg aattgtacga taagctgaaa tccactcata aagaagaaaa
     3541 aaacttgcca atgttttcaa ataccgattc agtacagata gatgttttct caagtaagtt
     3601 ttatagtttt tttgagcgaa actataaaag agttgccgct ataattttaa ccggattgct
     3661 gcttgtagtg cttatcatgt ctattagagc ccttatcgac aagagtatac aaggaaatat
     3721 ggagtcacag ccggaaactc ctacaacaca agcaacagct ccggaactgc aagatgatta
     3781 tcaggttgag aatatcccta ttgatgataa taaaagtgta atggttgaaa ttgtagagga
     3841 taccgctcac aataccattt ttataataga tgatgaacaa attgaagtta ccatggatga
     3901 tattatcgat aggtgctgga tttctgttca aaaggatggc caatttgatt ttgaaggtat
     3961 tttaaatccg ggtgatgcaa agacatggga agcaaaagac gctataaaca tgcggatagg
     4021 aaatccgcca gctgtaaatc ttattgtcaa cggcaatgac cttggtcggc ctcgagctgg
     4081 agctagagac ttcatattta aaaaaagaac ctaaagggtt cttttttaat tagcatcaag
     4141 atttatgttt catcttacat taactgtatt tacacattac cgcaaaacgg atatactaaa
     4201 atatcatatt agatgattgg aggagcaaat tgtcagcaaa aattggtttt atatctttag
     4261 gttgtgacaa aaaccttgtg gactcagagt acatgctagg aatcctaaaa aagcataatt
     4321 ataaaattac caacgatgaa gaagaagcag aaattataat catcaacaca tgcggtttta
     4381 taaatgatgc taaacaagaa tctattgata ccatattaga aacggctcag ctaaaaaaaa
     4441 caggcaaatt aaaacacctg attgctgcag gatgtcttgc tcagcgttac aaaagtgaat
     4501 tgttgcggga aattccggaa cttgatgcta ttgttggcac cggagatttt gacaagatac
     4561 atgaagtaat tgagcagcta gattcaaaac gaatcgagct tacggataat aagagctttt
     4621 tagattatga cattggaaat agaatcagag tttatccata tatatctttt gtgaaaattg
     4681 ccgaagggtg cgacaattgc tgctcatact gtgcaattcc gttaattaaa ggacattaca
     4741 aaagcagaaa aatatcaacg ataaaagaag aagttgaaaa cctagtcgct gaaggtgtca
     4801 aagaaatcaa tttagttgcc caagacacta caaactacgg agtggatatt tacggtaagc
     4861 cttcccttcc aaaactatta gaagaactag tgaaaatccg tgaagatttc atcataagaa
     4921 tactgtacgc ttatccaact aacatcaata aggaactgtt agacgtaatc aaaaacaaca
     4981 ataagattgc aaaatacctt gatattccac tacaacatat aaacgacagg atattaaaac
     5041 tcatgggaag gccaagcagc agtaagttta tcatgcagct tattgacgat ataagaaaac
     5101 aaataccgga aattacttta agaacaacat ttatcgtagg gtttcctaca gaaacggaaa
     5161 cggaattcaa tcagctattg gattttgtaa aactatatag atttgataaa gttggtgcat
     5221 tccagtattc caaagaagaa ggaacttttg ctgctgcttt aaatccagag gtgcccgaca
     5281 aaattaaata tagaaggtac agtgaattaa tggcggtcca gcagtctata tcaaaaacaa
     5341 aaaaccaaag atttaagggg aaagtgctaa aagtcttggt ggagaagtac gataaggaaa
     5401 atcggttgtt catcggccgt tcggagaatg atgcaccctt tgtagatggt gtaataaaaa
     5461 ttttagatgg caactgtgaa ttagggcagt ttcataaagt gaaaattatc gatagccagg
     5521 aatatgattt gctaggggaa ttggctaatt aatttttgtc agattatttt tataggcatt
     5581 gcaaaaaatg ccttttttct tttcaggaca acatttaatt gctatgctat aatattatta
     5641 tgtaaacaaa ggtgaggtga aaaaactttt ggatttagac aaaaccaaga ttctgcgaat
     5701 tcttgcgatt tcaagttttt ttctgttttt aatgttgtca ttacaatggt atttcggtaa
     5761 aaatgtaaag cttcgcgctc ttgaaagaga gctactggaa cataaatatg taagcagtgt
     5821 ggaaattaat gaacaaaagg aacaagtagt ggtttgccta agccttagga atatagataa
     5881 ttttaaggag gcatacgaca agatatgcga aactataaag gaacggctta aaatgcaacc
     5941 ttttgaactt aaaataatta acgaggccga ccctgaacta gaagatattt ttgacaataa
     6001 agttcagttc attgtatacg aggccttagc gaccggtgag tttacaaaaa tgcgagctag
     6061 tcttgatgaa atagagaata catctgattc tttaagagtt caggtgtttt tggattccag
     6121 caagcttttt ctgcacatac gacttaaaga atcaaattat tacagaatca tcaatagaga
     6181 agtgtaactc aagtactagg aggggagaag ctattgttac ttgaaatcgg aatagggctg
     6241 gggatagcat caataatata ccttgctata aacggcataa atgtcctgcc cgttattata
     6301 atgtttggat tctcatacat gctttacagc atcatgtcta gcaacaacct aataaaaagt
     6361 ccccaaaagt taataacaaa tgaacaaggt ataacttttg atcaaatagg cgggcaggaa
     6421 gtagctaaaa atgaattgat agaggcatta aatttcatta agaactttga tgaggcaaaa
     6481 aagatgggca ttcgccccct caaaggaata ttgctaacgg gagcacctgg tacaggcaaa
     6541 actcttatgg ctaaagccgc aacttcatat atagactctg tctttgtttc agcttcaggt
     6601 agtgaattta ttgagatgta tgcaggtgtc ggtgcacaaa gagtgaggca actgtttgaa
     6661 acggcacgga caatggcaag aaaagcttcc aaaaaacacg gcgttgtttt cattgatgag
     6721 at
//
LOCUS       DAONUO010000084         6683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8615_length_6683_cov_3.547526, whole genome shotgun sequence.
ACCESSION   DAONUO010000084 DAONUO010000000
VERSION     DAONUO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8615_length_6683_cov_3.547526"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK768_04300"
     CDS             complement(<1..199)
                     /locus_tag="PK768_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA60221.1"
                     /translation="MKKFKVGIIGAGRGGSALLETLIEMDNIEIQGIADIDSSAKAFG
                     AAKKAGIKCFTTVEELLSSCSL"
     gene            complement(355..1377)
                     /locus_tag="PK768_04305"
     CDS             complement(355..1377)
                     /locus_tag="PK768_04305"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQA60222.1"
                     /translation="MKKYHIAVVGATGAVGTEMLKILEERDFPIESIRLFAHESEAGK
                     KITFKGSEFTVEKVDEDNIKGIDIAFFAAGPVSKVLAPVFVKNGAVVIDNGSSFRLDP
                     SVPLVIPEINPEDVDWHSGIISNPNCATIILCVPLKPIYDYAGVTRIIVSTYQAASGA
                     GVKGIAELDEQVRSYAKGEPMPEPKAFTCPIAFNAIPHIDVFMEQDYTKEEWKTYYET
                     RKIFHDPDLRISSTTVRIPVMRSHCESILLETKKKITPDEVRDILSKAPGVKVYDDPS
                     NNIYPTPLTTQGQDIIYVGRIRRDTSSENGIWLWTCGDQIRKGAATNTIQIGEVLIER
                     GLLDKK"
     gene            complement(1613..2377)
                     /locus_tag="PK768_04310"
     CDS             complement(1613..2377)
                     /locus_tag="PK768_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQA60223.1"
                     /translation="MPRTAREKSKTGIYHIMLRGIDRRYIFLDDSDREKFLYYLFRAK
                     EISEFELYAYCLMGNHVHLLMRESGELGDNVKRIAVGYVQWFNNRHGRTGHLFQNRYR
                     SEAIESERYLVAAARYIHQNPVKAKMVKKAGDYEWSSYKDYIDFYKGKSTSIDPGLIA
                     AYFSTQESFEEFMNKPNDDKFLDYEVKQKYTDKDLLSRINGIVDINSLARLPKNERDK
                     IILEIYNMTGASMRQLSRVMGIGRSIIENAVRNSEL"
     gene            complement(2467..3108)
                     /locus_tag="PK768_04315"
     CDS             complement(2467..3108)
                     /locus_tag="PK768_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60224.1"
                     /translation="MNRDNLIIHQLLFFFGGLYIASLGKIFLMESGFGVDPWMVFHMG
                     ISNHTPFTVGRATQVVGALMLLIGWALKIKPGIGTVMNMYFFGFFLDLNIGINNALNL
                     VRPTESLIVSILYLIIGIIANGVGFGTYINGKLGAGPRDSFMLGISNVTGKKPGTIRT
                     VMESMAALIGWLLGGPLGIGTLAYALSVGTVMQWTLDHVKLPQRKQAPGPRPL"
     gene            complement(3105..3749)
                     /locus_tag="PK768_04320"
     CDS             complement(3105..3749)
                     /locus_tag="PK768_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60225.1"
                     /translation="MGKLKSIIPKLLFFLGGLYIVTVGMVIVKQSGLGVDPWMVFHMG
                     ISNYVPFTVGQVTQGVGAIMLLIGWALKIKPSIGTFLNMFCYGYFLDLNITINDTLNI
                     IQQTDSLIVSILYLLIGIIIIGVGLGLYLNGQLGVGPRDGFMVGISNVTGKKPGTIKT
                     WMECTAVFIGWLLGGPLGIGTLVYALSVGSVMQWTLDRVKLPQPKEVCEEGVAH"
     gene            complement(3838..5058)
                     /gene="truD"
                     /locus_tag="PK768_04325"
     CDS             complement(3838..5058)
                     /gene="truD"
                     /locus_tag="PK768_04325"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HQA60226.1"
                     /translation="MKVKVIPEDFIVTELIDLKLKNDGKYRIYLLEKRHWNTMDALLA
                     IARQNKMAMASIGYGGRKDRHAITYQYISVPKRYDLSFDMPNVKLSPVGFADDFISAA
                     LMKGNHFQITLRKIPEHQRESILERLDEISRFGFPNYFDDQRFGSIANPDDILAERII
                     RKHYKGALKLYFTTIGPGDKTKEKRRKRKIEELWGDWQAIAPLCKTKAERDIMKILWE
                     GDSKSQLVKAINAIPKEELSMHFSAYQSYLWNLSLEQILQKHVTNPFKVKGKIMDYTF
                     YKDLDEGALKKLKNMQIPTVSYKIPGNPEEARIVKDILSQRGLKPSDFNLTKIRKSFF
                     KSFLRPAIVYPENLTGGRFVCQVPDDVTEESMTEPLFEADDIYPGFSKLQLNFTLPTG
                     SFATMLIKSIQGTL"
     gene            5335..6195
                     /locus_tag="PK768_04330"
     CDS             5335..6195
                     /locus_tag="PK768_04330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQA60227.1"
                     /translation="MALVLVLAIGLGLIASACSSQASSEVVARVNNEKITKDELYEYL
                     VKENGDAALNTLVANKIIELEAKKQNVEVTDDDLDKEIDKIAEQYGGRETFEQFLEMY
                     GTPLDDIKETLRTNITVEKLLGAEVKIEEDEMKSYFEINKENFGEKEQVKASHILVDS
                     EEKAVEVKEKLEAGEDFAALAKEYSTDSSNSEQGGDLGYFARGQMVQEFEDAAFSMEV
                     GQISDPVKTDYGYHIIKLEDKKPAKQATYEESKDEIREILFQQKLPVVYQMWMQERMN
                     EYDVEILLNK"
     gene            complement(6255..6623)
                     /locus_tag="PK768_04335"
     CDS             complement(6255..6623)
                     /locus_tag="PK768_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQA60228.1"
                     /translation="MRKFLWATITVKDMEESLKFYQEIVGLTLDRRYQARPGVEIAFL
                     GSGETKVELIKDDSIEDVNIGEHISLGFEVDSVDGMMKFLGAKGIPIHSGPFQPNPSI
                     KFFYIKDPNGLNIQFFEHID"
BASE COUNT         1714 a         1492 c         1530 g         1947 t
ORIGIN      
        1 ccaagctgca ggatgacaaa agttcctcga cggtcgtgaa gcattttatg cccgcctttt
       61 ttgccgcgcc aaaagccttg gccgatgaat cgatatccgc tatcccttga atctctatat
      121 tgtccatttc aatcagggtc tccaaaaggg cgctaccgcc ccggccggcc cctattatcc
      181 ccactttgaa ttttttcaca ctgaccaccc cgtctgaagc ctcccaggaa gcaggttatt
      241 cgcattagag tcacttagga ttatttctgc acgaaataaa aaaaatcctc ctagtaaact
      301 aggagaaatt atagaaagct tgtaaaggag tggatgaaaa acaatatatt agatttattt
      361 tttatcaagt aagccgcgct cgattaacac ttctccgatt tgaatggtgt tggtagccgc
      421 gcctttcctt atctggtccc cacaggtcca cagccatatt ccattttcgc tggaagtgtc
      481 ccggcgtatc ctgcccacgt atatgatgtc ctggccctgt gtagtaaggg gcgtcgggta
      541 gatattgtta gagggatcgt cgtatacctt tactcccggg gcttttgaca ggatgtccct
      601 gacctcatca ggggtgattt ttttcttggt ttccaggaga attgattcgc agtgacttct
      661 cattaccggt atgcgcacgg tggtgctgct tatccttaga tcggggtcgt ggaagatttt
      721 gcgggtttcg tagtacgtct tccactcttc cttggtgtag tcctgttcca taaagacatc
      781 tatgtgaggg atggcgttaa aagctatggg gcaggtaaag gctttgggct ctggcatggg
      841 ctcgcctttg gcgtaggagc gaacctgttc gtccagctca gctatgccct tgactccggc
      901 tccggaagcc gcctggtacg tggatactat gatcctggtg acacccgcat aatcgtaaat
      961 gggctttaaa gggacgcaca ggattatggt ggcgcaattg ggatttgata tgatgccgct
     1021 gtgccagtcc acgtcttccg ggtttatttc tggtattacc agggggaccg acgggtccag
     1081 ccggaaggaa ctgccgttat cgatgaccac ggctccattt ttgacaaaga ccggggccag
     1141 taccttgctc acggggccgg cggcaaaaaa ggcaatgtct atgcccttta tattgtcctc
     1201 gtccaccttc tctaccgtaa actcactgcc cttgaaggta atcttctttc ccgcttcgct
     1261 ttcatgggca aaaagcctga tgctttctat cggaaagtcc ctttcttcaa ggattttcag
     1321 catttcagtg cctactgcac cggtagcacc aactactgct atgtgatatt ttttcatggg
     1381 gaaacctcca aagcaattag attttttaaa gataagtaat gcaaaatccg tgccaatctg
     1441 cagcgaaggc gcgcgaaagc ctacgatgct aattggcggt atcgagaacg caaaaatatg
     1501 cggcccgatt ctaactttta gaactacttt ttaaaaacta taacctgccg cggggacgtt
     1561 tcgtttgcca ggttcatctc accctgccac agggacgttt cgtttgccag gttcatagtt
     1621 ccgagttcct taccgcgttc tcaattatgc ttctccctat gcccataacc ctgcttaact
     1681 gcctcatgct ggctcctgtc atattatata tttccaagat aatcttgtca cgttcatttt
     1741 tgggcagtct ggccagtgaa ttaatatcca ctatgccatt aattctgctt agcaaatctt
     1801 tatccgtata tttttgctta acttcataat ccaaaaattt gtcgtcattt ggtttgttca
     1861 tgaattcctc aaagctctct tgtgtactga aataggccgc gattagcccc ggatcaatgc
     1921 tggtgctttt gcccttatag aaatcgatat aatccttata gctgctccat tcatagtccc
     1981 cagctttttt caccattttg gccttgacgg ggttttggtg tatgtacctt gctgctgcaa
     2041 caaggtatct ttcgctttcg attgcctcgc ttctgtacct gttttgaaag agatggccgg
     2101 ttctgccgtg cctgttgtta aaccattgca cgtagccaac tgctattctc ttgacgttgt
     2161 cgcccaattc tccgctttcc ctcataagca agtgtacatg gttgcccatt aggcaatagg
     2221 catataattc aaactcagaa atctcttttg ccctgaaaag ataatataag aatttttccc
     2281 tatcgctatc gtctaaaaat atgtaccttc gatcaatacc cctcaacata atgtggtaaa
     2341 tgccggtttt gctcttctcc ctcgcagttc ttggcattag catcaccctg ttgcattata
     2401 cttaattttc tggaatatgg cagacgaaac gtccccctgc caggtgccag gtgcaggtgg
     2461 cgggttttat agaggccgcg ggccaggggc ttgttttctt tggggcagct tcacgtggtc
     2521 aagggtccac tgcattacgg ttccaacact cagggcataa gccagtgtcc caatgcccaa
     2581 agggccgccc aaaagccagc cgataagtgc ggccatgctt tccatgaccg tcctgatcgt
     2641 gcccggcttt tttccggtaa cattggagat gccgagcatg aaactgtccc ttggcccggc
     2701 gcccagtttg ccgttaatgt aagttccaaa tccaactcca tttgcaataa tgccgataat
     2761 caggtataaa atagatacta ttaagctctc cgtgggtcta actaaattca aggcattatt
     2821 tatgcctatg ttcaaatcga gaaaaaagcc gaagaaatac atgttcataa cggtgccgat
     2881 accgggtttt atttttaagg cccacccgat taaaagcatt agtgctccga ccacctgggt
     2941 ggccctgcca accgtaaacg gagtgtgatt ggatattccc atgtgaaata ccatccaggg
     3001 atccacgccg aatccggatt ccattaggaa aatcttccct aaggaggcga tatataagcc
     3061 gccgaaaaag aataagagct gatgaataat caaattatcc ctgttcaatg tgctactcct
     3121 tcttcacaaa cttcttttgg ttggggcagt tttacgcggt caagcgtcca ctgcattacg
     3181 cttccgacgc ttaaggcata taccagtgtc ccaatgccga gaggaccgcc caaaagccag
     3241 ccgataaata cggctgtgca ttccatccat gtcttgattg tgccgggctt tttcccggta
     3301 acatttgaga ttccgaccat aaaaccgtct ctcgggccaa ctcccaattg cccgttaagg
     3361 tatagcccca atcctacgcc tattatgatg atgccgatga gtaagtatag gattgaaact
     3421 attaaactat ccgtttgttg tattatgttt aaagtgtcat tgatggttat attcaaatca
     3481 agaaaataac cgtagcaaaa catgtttaaa aatgttccga tgctaggctt tatttttaat
     3541 gcccagccta tcaagagcat tatggctcca accccctggg tcacctggcc aaccgtaaag
     3601 ggaacgtaat tagagattcc catgtgaaat accatccagg gatctacacc caatcctgat
     3661 tgcttgacaa taaccatgcc tactgtaacg atatatagtc cgcccaaaaa aaataaaagt
     3721 tttggaatga ttgatttaag cttgcccaat ttgctgctcc ttctaatgtt ttttattagt
     3781 catgtagttt tattatagca cgtagttata ggcaatacaa tttgcttgaa agctagttta
     3841 tagcgtcccc tgtatggatt ttatcagcat tgtggcgaag ctgcctgttg gcagtgtgaa
     3901 gttgagttgt agtttcgaaa agcccgggta gatatcatca gcttcaaaga gtggttctgt
     3961 catgctttct tccgtcacgt cgtcagggac ctggcagacg aaacgtcccc ctgtcaggtt
     4021 ttcgggatag actatggcgg ggcggaggaa ggatttgaaa aaggattttc ggattttggt
     4081 caggttgaag tcggagggtt ttaagccccg ctgggacaaa atgtctttta cgattcgggc
     4141 ctcctcaggg tttcccggga ttttataaga tactgtgggg atttgcatgt tttttagttt
     4201 ttttaaagcc ccttcatcta aatctttata gaaagtatag tccatgattt tcccttttac
     4261 tttaaagggg tttgttacgt gtttttgcag gatttgctct aaactcaggt tccacaagta
     4321 gctttgatag gcagaaaagt gcatggacag ttcttctttg gggatggcat ttatggcctt
     4381 taccagttgg cttttgctgt caccctccca aaggattttc attatgtccc tttcggcctt
     4441 ggttttgcac aggggggcaa tagcctgcca gtcgccccag agttcctcta tttttctttt
     4501 cctccgtttt tctttcgtct tgtctcccgg gccaattgtg gtaaagtaga gttttagggc
     4561 gcctttgtag tgctttctga tgatcctttc cgccaatatg tcgtcgggat ttgctatgct
     4621 gccgaagcgc tggtcgtcaa aatagttcgg aaagccgaag cggctgattt catccaaacg
     4681 ctccaggatc gattccctct gatgttcggg gatttttctt agggtgattt ggaagtggtt
     4741 tcctttcatt agcgctgcgg aaatgaaatc atcggcaaag cccacagggc taagtttaac
     4801 atttggcatg tcaaaggaaa ggtcgtatct ctttggcacg gaaatgtatt gataggttat
     4861 cgcatggcgg tcctttcggc cgccgtagcc tatgctggcc atggccatct tgttttgcct
     4921 ggcaatggca agcagggcgt ccatggtgtt ccagtgccgc ttttccaaaa ggtatatgcg
     4981 gtacttgccg tcattcttta gttttaaatc aatgagttcc gtcacgatga agtcttccgg
     5041 tattactttt accttcaata tcgctcattc cttttatcag tccttaaact tttctttgat
     5101 aatattatag tattagcatg aacctggcaa acgaaacgtc cccgtggcag gtttggtagg
     5161 ttgtgcgggt ttctattttg agtatatctc atttgccagg ttttttaaac aaaaagcaac
     5221 ggaaaaagtc tagacattag tagcacaaaa ctgataaact agtatgagca ataaatttta
     5281 ttaatagata gggtgaggga aatttttact aaaaaaatca gcataactaa gtctttggct
     5341 ttggttcttg ttttggcaat agggctcggc ttgatagctt cagcttgcag cagccaggca
     5401 tccagcgaag tggtggccag ggtgaacaat gaaaaaatca cgaaagatga gctgtacgag
     5461 tatttggtaa aggaaaacgg cgatgcggcc ctgaacacac tggtcgctaa caaaataatt
     5521 gaacttgagg caaaaaagca aaatgttgag gtcaccgatg atgatttgga caaggaaatt
     5581 gataaaatcg cagagcagta cggcggccgc gaaacttttg agcagtttct tgaaatgtac
     5641 ggcactccgc tggatgatat aaaggagact ctcagaacaa atattactgt cgaaaagctg
     5701 ctgggggccg aggtaaagat tgaagaagat gaaatgaaat cgtactttga aattaacaag
     5761 gaaaacttcg gcgaaaagga acaggtaaag gccagccaca ttttggtgga ctccgaggag
     5821 aaggccgtag aggtcaagga aaagcttgag gccggcgagg actttgcagc cctggccaag
     5881 gagtattcca cagacagcag caatagtgaa caaggcggag acctgggata ttttgcacgg
     5941 ggccaaatgg tgcaggaatt cgaggatgcc gctttttcca tggaagtagg ccaaatcagc
     6001 gacccggtaa aaacagatta cggatatcac atcataaagc tggaagataa aaagccggca
     6061 aaacaggcca catacgaaga aagcaaggat gaaataaggg aaatcctgtt ccagcaaaag
     6121 ctgcctgtag tttatcagat gtggatgcag gaaagaatga atgagtatga tgtggaaatt
     6181 cttttaaata agtaacggat ataaagcctt caggaacggt gaaaaatgct gtcttcctga
     6241 aggctttaac attgtcaatc aatatgttcg aagaattgaa tgtttaatcc gttggggtct
     6301 ttaatgtaga aaaatttgat actgggattg ggctggaagg ggccgctgtg gatggggatg
     6361 cccttggctc cgagaaattt catcatgcca tcgacggaat ccacttcaaa gcccagggat
     6421 atgtgttcac caatattaac atcctctatc gagtcgtctt ttattagttc aactttcgtt
     6481 tcgcccgatc ctaaaaaggc gatttcaact ccgggtcttg cctggtacct tctgtcaagg
     6541 gtgagaccca caatctcctg gtagaacttc aatgattctt ccatgtcctt gactgttatc
     6601 gtggcccata agaattttct cataagcaaa cctccttgtt taaaatgtaa tacaaatggg
     6661 gtgatagaaa tgaaaaccgt aaa
//
LOCUS       DAONUO010000085         6564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8816_length_6564_cov_4.044400, whole genome shotgun sequence.
ACCESSION   DAONUO010000085 DAONUO010000000
VERSION     DAONUO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8816_length_6564_cov_4.044400"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1811)
                     /locus_tag="PK768_04340"
     CDS             complement(168..1811)
                     /locus_tag="PK768_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQA60229.1"
                     /translation="MNPVIKLLQELKNNKGIRVGQTAVVSRKVHMINPAAPQKINIIV
                     NSDAGQVFYQRLSPNEIIHIDLFHEIKSFNLKQLAEFLVKTLERSPFNYLMDNIDIQT
                     GSGGGRLTGVLCYGRTESLIKSHLNHVHVAALISDEELESVFLIVNKIEQALLEQGVG
                     LRKVEKIQHEIGNSSVDVSSYASHSDSNLKQETDIFRDERFEETLEMIEHFGGLKQIE
                     DVLEVFHSKGNNQITLQALKKSCSDFDDIVKYLEESNFISKQQNLHYLTARGNELWEF
                     VKRNRRELECILKKTIKNLPKLDKCKGFDSSYFSKKASRGQRGPSICTPYVPEEWSAE
                     ISVPETVRRSIVRSYFENINFYLKQEDIICINRISKLHRDICLIIDASASMAGFRLRN
                     AKYLAKHLILMPQTRISILAFQDKRVNTFVPFTRNYEKLEGGLDKIVATGLTPLALAL
                     EKGAEYMSKKNLRNPLIILITDGIPTVPLWTSDPVNDAVSAAGKIFEKKISFCCIGLQ
                     PNKECLIKVTNTARGNLYVLDELNREVLVEVARQSYQLL"
     gene            complement(1811..3142)
                     /locus_tag="PK768_04345"
     CDS             complement(1811..3142)
                     /locus_tag="PK768_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HQA60230.1"
                     /translation="MKKYSHIVRHSGNLPLFDILDISVAGTLTGTPVHVHAEGLRGTG
                     KTTIIRAFKEVLPKINRIRDCLYNCDPEKPHCPQHRNLTPQQVRLIGVEQIDMPFLEI
                     SHSAKKGTVVGSIDLQKISSKDNPEAALLLGTIPKAHRGIIFVDEINRIADTSPEIVD
                     LLLDVMGTKPGRIQIEEAGFPVLEMPVQVSIWAASNPDEEPGPLEDVRRQLSDRFDFA
                     VRVERPANPWIIKKIIGGDTPAHDNDVVSKKRMQFLEVQKTIHQFEPTEDIINILSSM
                     YVDFGIESLRCIEACLLGIKIRSALLKQKPTTDDVVYISKFALRHRVNIKELNSILTN
                     LEKRQSSKEEVTSKANPPIEQTCKAKNTSKGVKTLKDKNQNASTYNLESFKNIFQRLA
                     ENFKSSKSERTNRSTSNFHSENYNVKAPPKKAVPIDKLDLTEYVKTEEELI"
     gene            complement(3251..3556)
                     /locus_tag="PK768_04350"
     CDS             complement(3251..3556)
                     /locus_tag="PK768_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60231.1"
                     /translation="MLKQLLYAGIGLAAITKEKAEELVAELVKKGEMSTDEGKDLLNS
                     LRSRIQEESDRLKERINEQVERTINSMNLVRKSDIDEVLDRLEKLEKRLDEIQSQEA"
     gene            complement(3573..4445)
                     /gene="secF"
                     /locus_tag="PK768_04355"
     CDS             complement(3573..4445)
                     /gene="secF"
                     /locus_tag="PK768_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQA60232.1"
                     /translation="MKSYKIMEKKHIWIGISVIMIALGIVSMSMRGLNWGIDFTGGNL
                     LHFNLNQNYELAEVRSILEELDIKEFEAKKAGDGQQELIIRTVELSENMQTEILDTLK
                     REWSETELIRAETIDAVIGKEMQRQAVIALIIANIGMLIYITFRFEFYSAVAAVMALL
                     HDVLIVLSFYAIFNIPVDSSFIAVILTIVGYSVNDTIVIFDRIRENLKVMKQASFEDI
                     ANISITQSITRTVNTSLTTLLTITALYFLGGETIRDFTSALIVGISAGTYSSIFIASS
                     LWLILRGKTRIANA"
     gene            complement(4458..5699)
                     /gene="secD"
                     /locus_tag="PK768_04360"
     CDS             complement(4458..5699)
                     /gene="secD"
                     /locus_tag="PK768_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQA60233.1"
                     /translation="MTSRIKSLAIILAIVVAAAVLAYSALSGIQIVGYRVIPIKEAIK
                     LGLDLRGGISILLEAKPKAGEIITDEKMAGAERVIRGRVDQLGVSEPMIVRQGDRRIL
                     VELPGVKDVQRAQELIGKTASLQFISPQNEVILTGDNVVDAKAVYGEQNEPMVSLKLD
                     SEGAKKFAEATKTYLEQHIAIMLDEQIISAPIVKSVITNGEAVISNVPSIEYAAELAA
                     LIRAGALPVDLEQRQVLMVGPTLGADSIDKSLKAGIIGVVLVLLFMLLYYRIPGLIAD
                     FALLVYILLVLTIFAGLGATLTLPGIAGFILSIGMAVDANVLIFERFKEELRNGKSLR
                     AATNAGFHRALTTIIDSNITTIIAAVVLLYFGTGPVKGFAVTLIIGIAASMFAAITVT
                     RVLLLNLINTRLFTNKKLFGA"
     gene            complement(5872..6021)
                     /gene="scfA"
                     /locus_tag="PK768_04365"
     CDS             complement(5872..6021)
                     /gene="scfA"
                     /locus_tag="PK768_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HQA60234.1"
                     /translation="MKHIVPLHKGNLNSKENLMHKGCGECHSSCQSACKTSCTVANQV
                     CSKEK"
     gene            complement(6108..6494)
                     /locus_tag="PK768_04370"
     CDS             complement(6108..6494)
                     /locus_tag="PK768_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HQA60235.1"
                     /translation="MKSKSNGKTSINLVKIFKGVFFAYLFTIFLFLVLAFIMYFSSIS
                     ENIIPKAVIIISAVSILLGGINTTKDLESMGWLHGGLVGFIYTGILIILSFIIVPSFA
                     LSINIAIDIFISFLVGVVAGIIGVSL"
BASE COUNT         1856 a         1440 c         1065 g         2203 t
ORIGIN      
        1 tccggttcaa aacatgaaat gatatttgtt tttaacatat ataattataa actaatacca
       61 tttttatatc aataacaaga actccacccg acaacgcaat gggagactct gtcggatgcc
      121 gggaaccttg ctgtttcaca aaaacaaaaa tcgagccgaa gctcgattta taacaactga
      181 taactctgcc ttgccacttc taccaatact tctcggttta gctcatccaa cacgtacaga
      241 tttccccttg cagtattggt tacttttatt aagcactcct tgtttggctg cagacctata
      301 cagcagaaac tgattttctt ttcaaatatt ttgccggctg cagaaactgc atcattcacc
      361 ggatccgaag tccacagtgg cacggtagga atgccgtcgg ttattagtat gatcagcgga
      421 tttctcaggt ttttcttgct catatactcc gccccttttt caagggctaa agctagaggt
      481 gtcaagcctg tggcaacaat cttatccaaa cccccttcca gcttttcata gtttctggta
      541 aaaggaacaa atgtattaac tcgtttatcc tgaaaagcca gtatgcttat ccgagtttgg
      601 ggcatcagaa taaggtgttt tgccaaatac ttggcatttc tcagtctaaa tcccgccatg
      661 ctggcactgg cgtctattat caaacaaata tcccgatgaa gtttggaaat tcgatttata
      721 catattatat cttcctgctt gagatagaag ttaatattct caaaataact ccttactatt
      781 gacctcctaa ctgtttcagg tacacttatt tctgcagacc attcttccgg tacatagggc
      841 gtacaaatgg aaggccccct ttgccctcta cttgcttttt ttgaaaaata gctgctatca
      901 aatcccttac atttgtcaag tttaggcaaa tttttgattg tctttttcag tatacattct
      961 aattccctac ggtttctctt aacgaattcc caaagttcgt tgccccgtgc agtgagataa
     1021 tggagatttt gctgttttga aataaaatta ctctcttcta aatactttac aatgtcatca
     1081 aaatcgctgc agcttttttt taaagcttgg agtgttattt gattgtttcc tttactgtga
     1141 aatacctcca gtacatcttc aatttgcttt aagcccccaa aatgctcgat catctctaac
     1201 gtttcttcaa atctttcatc tctaaaaata tctgtttcct gttttaaatt tgaatccgaa
     1261 tgacttgcat atgatgatac atccactgag ctgtttccaa tttcgtgttg gattttctct
     1321 acctttctaa gacctacacc ttgctccagc agtgcttgtt ctattttatt cactattaaa
     1381 aacacgcttt ccaattcctc atcggaaatt aaagctgcaa catgaacatg gtttaagtgg
     1441 ctttttatta gactctcagt cctgccatag cacaaaactc ccgttagtct gccccctcct
     1501 gaacccgttt gaatatcaat attgtccatt aggtaattga aaggacttct ttcaagagtt
     1561 tttactagga attcagccaa ttgttttaaa ttaaagcttt ttatctcgtg aaacaagtcg
     1621 atgtgaatta tttcattcgg agataaacgc tgataaaaaa cttggccggc atcggaattg
     1681 acaataatgt ttattttttg aggagcagcg gggttaatca tgtggacctt tctacttaca
     1741 acagcagttt ggcctacccg aatacctttg ttgtttttta attcttgcag tagctttata
     1801 actggattca ttatatcagt tcctcttcgg ttttcacata ttcagttaaa tccagtttat
     1861 ctataggtac tgcctttttc ggaggtgcct taacattata gttttccgaa tgaaaatttg
     1921 atgtgctcct atttgtcctt tcactctttg aagatttaaa gttttcggcc aatcgctgaa
     1981 atatattttt gaaagactct aaattataag tgctagcatt ttgattctta tctttaaggg
     2041 tttttacacc tttagatgtg tttttggcct tacatgtttg ttcaataggt ggatttgctt
     2101 tgcttgttac ttcttccttt gaagattgcc tcttttcaag gtttgtcaat atactgttta
     2161 attcttttat gtttactcta tgtcgaagtg caaacttgga aatgtaaaca acatcatcag
     2221 ttgtaggctt ttgttttaat aaagcgcttc ttatttttat tccgagcagg catgcttcta
     2281 tacagcggag gctctcaatt ccgaaatcca cgtacataga tgataatatg tttattatat
     2341 cttccgttgg ttcaaactga tgtattgttt tctgaacttc caaaaactgc attctttttt
     2401 tgctcaccac atcattgtca tgagctggag tatcaccgcc aattattttt tttataatcc
     2461 agggatttgc tggcctttca accctcactg caaaatcaaa tctatcagaa agctgacgtc
     2521 gaacgtcttc taatgggcca ggttcctcat cgggatttga cgctgcccat atactgactt
     2581 ggaccggcat ttccaaaacg ggaaaacccg cctcctctat ttgtatcctt cccggtttag
     2641 tccccataac atccagcagc aaatccacaa tttccggaga tgtatcagcg attctgttga
     2701 tttcatccac aaaaattatt cccctatggg cttttggaat tgtccctagc aaaagtgccg
     2761 cctctggatt atccttagat gaaattttct gtaaatcaat acttcctact accgtcccct
     2821 tttttgcaga atgtgagatt tcaagaaagg gcatatcaat ctgttcaacg cctattaatc
     2881 gtacctgctg aggtgttaaa ttgcgatgtt ggggacagtg aggtttttcg ggatcacaat
     2941 tgtataaaca atccctgatt ctgttgattt taggcagtac ttctttaaag gctcttataa
     3001 tagtagtttt gcctgtaccc cttaatcctt ctgcatgaac atgaactgga gttccagtca
     3061 atgtgcctgc aacggatata tccaatatgt caaaaagggg aagattgcca gaatgtctta
     3121 ctatatgaga gtattttttc atactgcatc cctcacgaca attctttttt aaacctacta
     3181 tgtatttttt ccgaaaaact catttttata agagtaaagc aagatgttcc aaaacaaata
     3241 agaggccttt ttaagcctct tgactttgaa tttcatccag tcttttctct aatttttcaa
     3301 gtctgtccaa gacttcatct atgtctgatt ttcggaccaa attcattgag ttaatagtgc
     3361 gctctacctg ttcattaatc ctttctttca gcctgtcact ttcttcctgt atcctgctcc
     3421 tcaatgagtt taacaaatct tttccttcat ctgttgacat ttcacccttc ttaaccaatt
     3481 ctgctactag ctcttcggcc ttctcttttg ttattgcagc aagaccgatg ccggcataaa
     3541 gcagttgttt taacatggta tcacctcatt ttttatgcat ttgctattct ggttttgcct
     3601 ctcaaaatta accataggga actagcaata aatatagaag agtaagttcc tgcacttata
     3661 cccacaatta atgccgaggt aaagtccctt attgtttcac cgcctaaaaa gtacagtgcg
     3721 gtaatcgtta aaagggttgt cagcgaagtg tttactgtcc tcgttatgct ctgagtgatg
     3781 cttatattcg ctatatcctc gaaggaggct tgtttcatga ctttgaggtt ttcccttatc
     3841 ctgtcaaaaa tgactatggt atcgttgacg gaataaccca ctatagtgag aataacggct
     3901 ataaagctgc tatctaccgg gatgttaaaa atagcataaa aactcaagac aataagcaca
     3961 tcgtgcagca aagccattac tgcagcaact gcagaataaa actcgaatct aaatgtaata
     4021 tatataagca ttcctatatt agcaattatc aaggctatga cggcttgcct ttgcatctct
     4081 tttccaatga cggcatcaat agtctctgct cttatgagct ctgtttccga ccactctcgt
     4141 ttcaaagtgt caagaatttc tgtttgcata ttttcagaca gttcaacggt cctgataatt
     4201 agctcttgct ggccatcacc ggcctttttt gcctcaaatt ccttaatatc tagttcctct
     4261 aaaatgctcc taacttctgc cagttcataa ttttgattca gattaaaatg aagtaaattg
     4321 ccgccggtaa aatcaatgcc ccagttgaga cctcgcattg acatagatac aatacccaga
     4381 gcaatcatta taacggatat gccaatccat atatgctttt tttccattat cttatatgat
     4441 ttcacttttt ccacctctta tgcgccaaat aattttttgt tggtaaacag tcgcgtgttt
     4501 atcaagttta ataagagcac tcgggttacc gttattgcag caaacatact agctgcaatg
     4561 ccaataatta aagtaacggc aaatcccttt acgggaccgg tcccgaagta caggagcacg
     4621 acagctgcta taattgtggt aatgtttgaa tctattattg tagtaagggc tctgtgaaat
     4681 ccggcattcg ttgcggctct taaacttttt ccgtttctca attcttcctt aaatctttca
     4741 aaaatcagaa cattggcatc tactgccatt cctatagaaa gtataaagcc agcaataccc
     4801 ggcaaagtca aggttgcacc taaacctgcg aatattgtca atacaagcaa tatatagacc
     4861 agcaatgcga aatctgctat aagccctggt atcctgtaat acaaaagcat gaataataat
     4921 accaaaacaa caccaattat accggctttt aaactcttat ctattgaatc agcacctaga
     4981 gtaggcccaa ccatcaatac ctgacgctgc tcaagatcaa caggcagtgc cccggcccga
     5041 atcagcgctg ccagttcagc ggcatattct atactaggaa cattgcttat gactgcttcg
     5101 ccgttagtaa taaccgattt tacaatgggc gctgaaataa tctgctcatc gagcattatt
     5161 gctatgtgct gctcgaggta tgtcttcgta gcttcggcaa actttttggc accttccgaa
     5221 tctagtttta aactaaccat tggctcattc tgctctccgt atacagcctt agcatctaca
     5281 acattatctc ctgtaagaat cacttcattt tgcggactta taaattgaag agaggctgtt
     5341 ttgcctataa gctcttgagc tctttgcaca tcttttacac cgggaagctc aactaatatt
     5401 ctcctatctc cctgcctcac tatcataggc tctgatactc ctaactggtc aactctaccg
     5461 cggatgactc tttctgctcc tgccattttt tcatcagtta ttatctcacc tgctttaggc
     5521 ttagcctcta agagaattga aatgccacct ctcaaatcca gtccaagttt tattgcttct
     5581 tttatgggaa ttactctgta ccctacaatc tgaataccgc tcagcgctga ataggctaac
     5641 acggcagcag caacaactat ggccaaaatt attgctaggc tttttattct tgaagtcatg
     5701 ttggatttct gcccccattc tcaattttaa tgcattttaa attatatcct cccggcaaat
     5761 gccttgtcaa caaagccaat aatttacaaa aaaaaggaca ggcctatcaa tagtttgaat
     5821 aaaagcatat catttaatac tcatcaaatt attagcttgt aaagacaatt cttatttttc
     5881 ctttgaacac acttggtttg caacagtaca agaggtcttg cacgctgact gacaggaaga
     5941 atggcattct ccacatccct tatgcattag gttttccttg ctgtttaaat tgcctttatg
     6001 taatggcaca atatgcttca taactcctgc ctcctctttt tttattatgt gtaattatac
     6061 catattacaa gcgaatataa aagggaaaat tagtcaattg acgctaacta aaggctcaca
     6121 cctataatac ctgccactac tcctaccaaa aagcttataa aaatatctat agcaatatta
     6181 atactaagag caaaagatgg cactattata aagcttagga ttatcaaaat tcccgtataa
     6241 ataaaaccca ccaagcctcc atgaagccaa cccatacttt ccaggtcttt tgtggtattg
     6301 ataccgccca aaagtatact cactgcgctg ataatgataa cagctttggg aatgatattt
     6361 tcggatatgc tcgaaaaata cataataaat gccaaaacca aaaacagaaa tatcgtgaac
     6421 aaatacgcaa aaaatactcc tttaaatatt tttacaaggt ttatacttgt ctttccgttt
     6481 gactttgatt tcacgccaac tccctcctct ttactatact ttatttatca gaggagggga
     6541 aatatgacaa aaagcaagag attt
//
LOCUS       DAONUO010000086         6538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8860_length_6538_cov_3.993059, whole genome shotgun sequence.
ACCESSION   DAONUO010000086 DAONUO010000000
VERSION     DAONUO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8860_length_6538_cov_3.993059"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PK768_04375"
     CDS             complement(<1..407)
                     /locus_tag="PK768_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60236.1"
                     /translation="MEALQQKMPFYSIVFASTRYWPNATEMQQAIFEITRALTNDLQK
                     HDPGIAIKNITISSEDELEEFLRLKPEGFGLLMPLRGAVQPWMLKAAENFEGIGMLAA
                     YEPGCLEEKTAYRLLEANAAPAVADVYSVIKRKG"
     gene            complement(436..2397)
                     /locus_tag="PK768_04380"
     CDS             complement(436..2397)
                     /locus_tag="PK768_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQA60237.1"
                     /translation="MIETTMKVKDYNIRVYSLNTVVVGTGAAGFNAADRLVSYGQKDV
                     AIVTEGINAGTSRNTGSDKQTYYKLTLSGEEPDSIRELANTLFEGQCMDGDIALCEAA
                     LSAQCFLKLVELGVPFPRNRYGEYIGYKTDHDPRRRATSAGPYTSKFMTECLEKAVRE
                     KNVRIFDKLQVIKVVTKDNRLFGLLCIDLENLDDPDMRYVAFNCKNVIYATGGPAGMY
                     ADSVYPLGHYGATGIAFEAGVMGKNLTEWQYGLSSINPRWNVSGTYMQVLPKFISTDE
                     NGNDEREFLFDFFTDKGDMLSKVFLKGYQWPFDVRKIDNGSSIIDILVYIETCVKGRR
                     VFLDFRDNPGGEEIDFSILAPEAREYLERAGATFGTPIERLMHMNAPAVSFYKDKGVD
                     LHKEPLEIALCAQHNNGGLAIDKWWQTNIEGFFAAGEVSGSHGVYRPGGTALNSGQVG
                     STRAAQFISAKRKGEPADIDTFKDVAFAPISDAIAMGEAVLSDDDTVEKIWQNSAKRM
                     SRFGAAIRSIEDIRKVTEEIKEELANFGSIVKTASANKLHKVYRLYDILICQYVYLNA
                     MVDYVEKGGKSRGSALYTDKNGYLPYDNLPDMFKYTLDDGSRSDMVQEVIYKNGRCEF
                     MWRKVREIPVEDDFFENVWRSYRKNGNIY"
     gene            complement(2448..3215)
                     /locus_tag="PK768_04385"
     CDS             complement(2448..3215)
                     /locus_tag="PK768_04385"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HQA60238.1"
                     /translation="MRKTAIVTGGGRGIGFATAYQLGKDGYNIVIMGRSSEESYKENL
                     DLLAREGIEYLYVQGGIDKAEDRQRCVDEAIKKFQRIDVLVNNAGVAPKVRADLLEMT
                     EESFDRVVGINTKGTMFMTQLVAKQMLKQELLGSKRGTIVNVSSISAEVTSTNRGEYC
                     VSKAGISMLTKLYADRLAGEGIYVYEVRPGVIATDMTAGVKERYDELIEQGLFPIKRW
                     GKPEDVANVVSALCSDKFLYTTGIYINVDGGFHIQRL"
     gene            complement(3242..4270)
                     /locus_tag="PK768_04390"
     CDS             complement(3242..4270)
                     /locus_tag="PK768_04390"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQA60239.1"
                     /translation="MRILKNRVGRVIEPGKVDFLLRTVDEPKEQQVVIKIASSAICGS
                     DLHIFKGKHPSAPLPVTIGHEFAGEVVAVGSKVSKVKIGDRVTVEPVIVCGKCRACRT
                     GNYGYCENISFTYRVGDGAMADYITVEEPYVYKLPEHLSYSAGALIEPLAVAVHAVRR
                     ADIKLGEKVLVIGAGAIGILVAALCRRRGAAEVAIADFSQKRLNIALELGATMAINPS
                     KQNVYDVVSKITDGVGMDKTFECVGLEATFNQAMMALRKNGLATIVGIFENPNITIPA
                     TRFITHEIRVQGSQGYCWDFPIALEMSKEIDLEKLITHTFKLEDLQQALETCLDRDSG
                     ALKVIIHP"
     gene            complement(4286..5644)
                     /locus_tag="PK768_04395"
     CDS             complement(4286..5644)
                     /locus_tag="PK768_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60240.1"
                     /translation="MATFERFKFKSVDEIREKVKELGVNINFSDDLSPLANPVKIGHV
                     ESPNSIAVLPMEGCDSERDGSPSDLVRRRYRRFAAGGAGLLWFEACAVVHEGRANPLQ
                     MYLTEKNVGAFQELLKEVDREAAKYNGSDHRPVKVLQLTHSGRYSRPDDLPAPIIAQH
                     DPLLDPRVGLSPDDPVVTDEYLDVLIEKYVKSAILAREAGFDGVDIKACHRYLLSELL
                     ASHTREGRYGGSFENRVRFLIETIKAVRREVGNDFIIASRFNAFDAHPYPYGFGVDKE
                     DFWKPDLTEPLALVKLMVENGVDLLSSSAGNPYYKQPYVTRPFDQPTIGGELPEEHPL
                     ETVARLFGLTRQVQKSAGNIPVVGNGYSWLRQFLANAGAANISDGSCAIVGIGRQAFA
                     YPDAPRDILEKGAMDPKKTCISCSKCTQIMRDHGRTGCVIRDSEVYLPLYNEARSNAT
                     KK"
     gene            complement(5689..>6538)
                     /locus_tag="PK768_04400"
     CDS             complement(5689..>6538)
                     /locus_tag="PK768_04400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQA60241.1"
                     /translation="LHIPAMARQAGIDITPELFDRISREVPVISTIYPNHPTYTMVDF
                     SNAGGLGAVLAELAEEGLINTDARGMFGTIADKIALSQNLNRDVIRPVSEPIYEQGGL
                     AVLSGNIATESAIVKFSAVEREAWQFTGPAKVYDSQDDAWNAILRDEIVPGDVVVIRY
                     EGPKGSPGMPHMETFMAAVLGKGLGTKIALVSDGRFSGATGGLAIGHVSPEAFEGGNI
                     ALIKNGDIIHIDIEKRILMADVSDEEFKERKKAWKPVEKPAAGWLKLYRKNTTSAHKG
                     ATVYWD"
BASE COUNT         1559 a         1692 c         1361 g         1926 t
ORIGIN      
        1 ccttttcgtt taatgacact gtatacgtca gctactgccg gtgccgcatt ggcttccagc
       61 aacctgtatg ctgtcttttc ctcgagacat cccggctcat acgctgccag cataccaata
      121 ccttcgaaat tttcagccgc ctttaacatc cagggctgga ctgctcctct gaggggcatt
      181 aataggccaa agccttccgg ctttagcctc aaaaactctt ccagttcatc ttctgaggaa
      241 atggtgatgt tttttatggc aattccgggg tcgtgttttt gcaaatcatt ggttagggct
      301 ctcgtaattt cgaatatagc ttgctgcatc tccgtggcat ttggccagta gcgagttgat
      361 gcaaaaacaa tcgagtagaa tggcattttt tgttgtagtg cctccattat cattctccct
      421 ttttgatttt atgatttagt atatgttgcc gtttttcctg tagcttctcc aaacgttttc
      481 aaagaagtcg tcttctaccg gaatctctcg gactttacgc cacatgaatt cacaccggcc
      541 gtttttgtat atgacttcct gcaccatatc gcttcttgaa ccatcatcga gagtgtattt
      601 gaacatatcc ggtaggttat cgtagggcaa atagccgttt ttgtcggtat atagagctga
      661 acctctgctc ttgccgccct tttccacgta atccaccatc gcatttaaat acacgtattg
      721 gcatatgaga atatcgtaaa ggcgatatac tttgtgaagc ttgtttgcgg aagcagtctt
      781 gacgatactg ccgaaatttg cgagctcctc ctttatttct tccgtcacct tgcgtatatc
      841 ctctatgctg cggatggcag cgccaaaacg gctcatgcgt tttgcggaat tttgccagat
      901 tttttctacg gtatcatcat ctgaaaggac tgcctcaccc atggcgatgg catcgctgat
      961 tggagcaaat gccacatctt taaatgtatc tatatcagca ggttcccctt tgcgttttgc
     1021 ggaaatgaat tgggctgccc ttgttgagcc gacctggccg gaattgagtg cggtgccccc
     1081 cggacgatac acgccatgac tgccgctaac ctcccctgct gcaaagaaac cttcaatatt
     1141 tgtctgccac catttgtcaa tggcaaggcc accattgttg tgctgggcgc aaagggcgat
     1201 ttctaagggc tccttgtgaa ggtccacacc tttgtccttg taaaagctca cagccggagc
     1261 gttcatgtgc attagccttt caatgggtgt gccaaaggtg gctcctgccc tttcgaggta
     1321 ctcccgtgct tcaggtgcca gaatcgaaaa gtctatctcc tcgccgcccg gattgtcgcg
     1381 aaaatcaagg aatacccgac ggcccttgac gcatgtctca atgtacacca gtatatcgat
     1441 gatggatgag ccattgtcta ttttgcgaac gtcaaagggc cattggtaac cctttaaaaa
     1501 caccttgctt agcatatctc ctttatcggt gaaaaagtca aagaggaatt cccgctcatc
     1561 attgccattt tcatcagtgg aaataaactt cggcagtacc tgcatgtaag tgccggaaac
     1621 attccagcgg ggatttatgg atgataagcc gtactgccat tcggtgagat tcttgcccat
     1681 aacccctgct tcgaaggcta tgccagtggc tccgtaatgg cccaggggat agaccgaatc
     1741 cgcatacatg cctgccggac cacccgtagc atatattacg tttttgcaat taaaggcgac
     1801 ataccgcatg tctggatcgt caaggttttc taagtcaatg cacaaaaggc caaatagcct
     1861 gttgtcctta gtcactactt taataacctg cagcttatca aaaatcctta cattcttttc
     1921 ccgcaccgct ttttccaagc attcagtcat aaatttggag gtgtagggac cggctgaagt
     1981 tgcccgccgc cgtggatcat ggtctgtctt gtagcctata tattcaccgt acctgttgcg
     2041 tgggaaggga actcccagtt ccacaagttt taaaaagcat tgcgccgaca gtgccgcctc
     2101 gcagagggca atgtcaccgt ccatgcactg gccttcaaat aaagtgttgg caagctccct
     2161 aatggagtca ggttcttctc cggacagtgt taatttataa taggtctgtt tgtcagaacc
     2221 ggtgttgcgc gatgtgccgg cattaatgcc ttcagttaca attgctacgt ccttttgtcc
     2281 gtaacttacg agccggtcag ccgcattaaa acccgctgca ccggttccca ctacaaccgt
     2341 atttagagaa taaaccctaa tgttgtagtc ctttaccttc attgttgttt caatcatatc
     2401 cctcatcctc cagcttttag tatttcccgg caaatgacgg gattctgtta cagtctttgt
     2461 atgtggaatc ctccgtccac atttatatag atgcccgtag tgtataggaa tttgtcagaa
     2521 caaagtgcag aaaccacatt tgctacatct tccggcttgc cccaacgctt tatcgggaat
     2581 aatccttgct ctataagctc atcatatctt tctttaactc ctgcagtcat atccgtagcg
     2641 ataactccgg gtctgacctc atatacgtat ataccttcgc cggccagcct gtctgcatat
     2701 agttttgtca gcatggaaat gccggccttg gaaacgcagt attcgcctct gttggtagaa
     2761 gtcacttcag ctgaaatcga agaaacattt actatggtgc ctctcttgga acccaacaat
     2821 tcctgtttta acatctgttt ggcaaccagc tgggtcataa acatggtgcc tttcgtattt
     2881 atacctacga ctctgtcaaa gctctcttcc gtcatttcaa gtagatcggc tctcactttt
     2941 ggtgcaacac ctgcattgtt taccaagaca tctatccttt gaaacttttt aatggcttca
     3001 tcaacacatc tttgcctgtc ttccgcttta tctattccac cctgaacgta aaggtactca
     3061 ataccttctc tcgctagcaa atcaaggttt tccttgtagc tttcttcgga gcttctgccc
     3121 ataataacta tattgtagcc atccttaccc aactggtaag ctgttgcaaa acctatacct
     3181 cggcctccgc cggtaacgat agcagttttc ctcatatgca tactcctttt ttgccaaatt
     3241 attatggatg aataataacc tttagtgcac ctgaatccct gtcaaggcaa gtttcaagtg
     3301 cctgctgcaa atcctctaat ttaaatgtat gtgtgattaa tttctcaagg tcgatttctt
     3361 ttgacatttc cagggcgatg gggaaatccc agcagtagcc ctgtgaaccc tgaaccctaa
     3421 tctcatgggt aatgaagcgc gtggccggga tagttatgtt tggattttca aagatgccga
     3481 ctattgtagc taaaccgttt ttcctcaggg ccatcatagc ttggttaaaa gttgcctcta
     3541 ggcctacgca ttcgaaagtc ttatccattc caactccatc tgtaatttta gagacaacat
     3601 catatacatt ctgcttcgat ggatttatag ccatagttgc cccaagttcc agggcaatat
     3661 ttaaacgctt ttgagaaaaa tctgcgatgg caacttctgc agcacccctc cttctgcaga
     3721 gggctgctac taaaatgcct attgctccgg cgcctattac aagcaccttt tcccccagct
     3781 ttatatcagc tctccttact gcatgaacgg caactgctag aggctcaatt agggcacctg
     3841 cactgtaaga caggtgctcg ggcaatttgt acacataggg ctcttccact gttatataat
     3901 cggccatagc tccgtctcct acccggtagg tgaagctgat attttcgcag tatccgtagt
     3961 tgcccgtcct gcaagcccgg catttcccgc aaacaatgac cggttctacc gttaccctat
     4021 cccctatttt cactttactg accttgcttc cgacagcaac tacttcacct gcaaactcat
     4081 ggccgatagt taccggtaag ggtgctgaag ggtgcttgcc cttaaaaata tgcaggtcac
     4141 tgccgcagat ggcactagat gcgattttta ttacaacctg ctgctccttt ggctcatcaa
     4201 ctgtacgcaa tagaaaatct accttacccg gctctattac tctaccgact ctatttttta
     4261 aaattctcat attttcctcc aaagtctatt tcttggtagc attacttctt gcttcattgt
     4321 aaaggggaag ataaacctcg ctgtccctta tgacacagcc cgtgcggccg tggtcacgca
     4381 tgatttgagt gcatttgctg caggaaatgc aagtcttttt cgggtccatg gcaccttttt
     4441 caagtatatc tctcggtgca tcaggataag caaatgcctg acggccaatt ccaactatgg
     4501 cacaggaacc gtctgagata ttggctgcac ctgcatttgc caggaattgc ctaagccagc
     4561 tatagccatt gcctaccacc gggatattcc cagcagactt ttgaacttga cgggttaacc
     4621 caaaaagacg tgcaactgtt tccaaggggt gctcttcagg aagctcacca cctatagttg
     4681 gttggtcaaa gggtctggtt acgtacggct gcttatagta tggatttcct gcagaactgc
     4741 tgagaaggtc tacgccgttt tcaaccatca gctttacaag ggccaatggc tcggttaaat
     4801 ccggcttcca aaaatcttcc ttatcaacgc caaagccata aggatatgga tgagcatcaa
     4861 atgcattaaa gcggcttgcg attataaaat cattgcccac ttccctgcga actgccttaa
     4921 ttgtttctat gagaaagcga acgcgatttt cgaaacttcc accatatctg ccttctcttg
     4981 tgtgacttgc aaggagttca gatagcaggt acctgtggca agcctttata tcgacaccgt
     5041 caaaacctgc ttcccttgcg agaatcgcag atttcacgta tttttcaatc aacacgtcca
     5101 aatattcgtc agtcacaacc ggatcatcgg gggaaaggcc cactctcggg tccagcaagg
     5161 gatcgtgctg tgctataata ggtgccggaa ggtcatcggg ccttgaatac ctgccggaat
     5221 gtgtgagttg aaggactttc accggcctgt ggtcgctgcc gttatatttt gccgcttccc
     5281 ggtccacctc ttttagaagc tcttgaaaag ctcctacatt cttttccgtc agatacatct
     5341 gaagggggtt agcccgccct tcgtgcacca ccgcacaggc ttcaaaccac aaaaggcctg
     5401 ctccgcctgc agcaaaccga cgatagcgtc tcctcacgag atccgaggga ctgccatcac
     5461 gttcactgtc acagccttcc atgggcaaaa ccgctataga gttggggctt tcaacatgcc
     5521 ctattttaac gggatttgca aggggtgaaa ggtcatctga aaagtttatg tttacaccca
     5581 gttccttaac cttttccctt atctcatcta cgcttttaaa cttaaaacgt tcgaaagtcg
     5641 ccacttgaat acactccttt aaattgccgt ttttcaatgc aatactttct aatcccaata
     5701 aaccgttgca cctttgtggg ctgatgttgt gttttttctg tataatttta gccaacctgc
     5761 agccggcttt tctacaggtt tccaggcttt tttccgctcc ttaaattctt catcgctgac
     5821 atctgccatt aaaatgcgct tttctatatc aatgtgaatt atatctccat ttttaattaa
     5881 ggctatattt ccgccttcaa aagcttcagg tgatacgtga ccaatggcaa gaccgcctgt
     5941 ggccccggaa aatctcccat ccgaaacgag ggcaatctta gttcccagcc ccttgcccag
     6001 gaccgctgcc atgaaggtct ccatgtgggg catgccaggt gaacctttcg gaccctcata
     6061 gcggatgacc accacatccc ccgggacgat ttcatctctc agtatggcat tccaggcatc
     6121 gtcctgggag tcatatacct ttgcgggacc ggtaaattgc catgcttctc tttcaacagc
     6181 tgaaaatttc acgatggcac tttcagttgc aatattacct gaaagcacgg caagtccccc
     6241 ttgttcataa atgggttccg atacgggtcg aattacatcc cggtttaggt tttgtgagag
     6301 ggcaatttta tcggctattg tgccgaacat tcccctcgca tcggtattaa ttaagccttc
     6361 ctcggcaagc tcagccaaga ctgcgccaag gcctcccgca ttagaaaagt ctaccatcgt
     6421 gtatgtgggg tggtttgggt atatggtgct aattaccgga acttcacggc ttatcctgtc
     6481 aaaaagttcg ggtgttatgt caattcccgc ttgacgcgcc atcgcaggta tatgcaga
//
LOCUS       DAONUO010000087         6534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_8869_length_6534_cov_3.875907, whole genome shotgun sequence.
ACCESSION   DAONUO010000087 DAONUO010000000
VERSION     DAONUO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8869_length_6534_cov_3.875907"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..773)
                     /gene="nagB"
                     /locus_tag="PK768_04405"
     CDS             complement(39..773)
                     /gene="nagB"
                     /locus_tag="PK768_04405"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQA60242.1"
                     /translation="MQIFIEKDYSAMSKKAAQIFANGIQQKPDLVLGLATGSTPIGTY
                     QELIRMHKEEGLDFSKAVSFNLDEYYGLASDNPQSYNYFMFENLFNHVNIKKENVHIP
                     NGLVSDVETYCKQYDEEIEKYGGIDLQLLGIGVNGHIGFNEPAEELVLGTHLTDLTED
                     TIKANSRFFDSPEEVPTKAITMGIGSIMKAKKILLLASGKNKAEIMAKLLNSDVVTTK
                     VPASLLMLHPDVTIIMDEEAASLCKR"
     gene            1118..2617
                     /locus_tag="PK768_04410"
     CDS             1118..2617
                     /locus_tag="PK768_04410"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HQA60243.1"
                     /translation="MGILARKFTEVSYSVLPITVLVIILHFTLIPIETEMLARFIIGA
                     ILVIIGLGIFLFGAEIGIVPIGNLMGETIAKTNNSYLVGILGFILGFLITVAEPDLQI
                     LARQVNMASGGIISDLLILIVVSIGVGIMVGIGLLRILYEKPLNRLLTLVYFIIFLLG
                     IFTSQEFLAISVDASGATTGAMTTPFILALGYGVSQLKGGKTSEEDSFGMVGLASAGP
                     IFAVMAMSIISGLKDIAGTAEAFVPNTGILQPYFHAFPKFLKESLLTLFPLFVLFMVF
                     DKAKFKLNKKHKHAVLKGLLYTFIGLTLFLVGVNAGFMEVGRVMGQGIAKDYFWLLPV
                     IGFFLGMVVVLAEPAVYVLTEQVEDVTAGHIKKKAILATLSIGIAFAVVFSMMRIMIP
                     SLKLWHFLLPGFAFAAFLSYRVPPIFVGIAYDSGGVASGPMTATFVLAFAQGAANAIP
                     TANVMVDGFGVIAMVAMMPLVAIQILGLIYKMKAGEDGQSQSYEEAQSS"
     gene            2614..3282
                     /locus_tag="PK768_04415"
     CDS             2614..3282
                     /locus_tag="PK768_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQA60244.1"
                     /translation="MSPTGKNLVLFCCIVDCGKGSKVLKIAKKVGAVGGVVFLGKGTV
                     RDELLKFLGILDVRREIFLSVIDEELEDALYNEMNKKLGLNKPKHGIAFSIPLKSLVK
                     IDEREYKSKTEVKGVAKVSYEAIFVIVDKGSLDDVIDAAEAAGSTGGTVIHGRGLGNH
                     EKARLFNIEIEPEKDIVLILSKVDKTEDIVNAIREKLNIDHPGTGIIFVLDVKRTVGL
                     YEDN"
     gene            complement(3283..4323)
                     /locus_tag="PK768_04420"
     CDS             complement(3283..4323)
                     /locus_tag="PK768_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQA60245.1"
                     /translation="MIINNECRNREFDVIGFGEVMLRLSPPNKERISQGEVFEKRAGG
                     SEFNVVSGISMLGLRTGIITKLPDNEIGKFIKNKIRFHGVSDDYVIYDQSECKRLGIY
                     YFEGGAFPRKPTVVYDRSGSSFTTISADEIDSSVYSSSKIFHVSGISLALGVNVRETV
                     IDMIKKFKKHGAAISLDVNYRATLWDEETARKVITSIFPDVDILFVSEETSRRMLQRT
                     GTLEDIMKGFARDYGIKIIATTERKAKSPTKHTWDSRIYNALEGKFYKEDPYIDIEVV
                     DRIGSGDAYVAGVLFGIVKFNCLQKALEFGNAMAALKNTVPGDLPACDFKEIEQLIAS
                     HKKTGTDSEMNR"
     gene            4647..5504
                     /locus_tag="PK768_04425"
     CDS             4647..5504
                     /locus_tag="PK768_04425"
                     /inference="COORDINATES: protein motif:HMM:NF018703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60246.1"
                     /translation="MADLEKFIKDMEKASEKLADMLNKKEEPIPEVDSEFSTEVEELQ
                     EGHEIAEIDEDSRVIVKNADVADCGCDETEQNQATPQPCNRIVRFNCIENIPRGLRLD
                     RVRDFRVVYDPTDLKVCVEEAEISVTPPPGCPDLTLSVFAVRVIGCIPVSISVLAFEG
                     RCGVNLVPRRDRDDRVALCCHTTVCVDNVICYRGTREQAEFAAAEIRRELSSGKQCGV
                     TVSETEDEEAAAQFIKGRRDPCNAVPLLFAIGRIFRVPVGQDNDDPKILAFSGAFRLP
                     ACPEANAAG"
     gene            complement(5609..>6534)
                     /gene="mtnA"
                     /locus_tag="PK768_04430"
     CDS             complement(5609..>6534)
                     /gene="mtnA"
                     /locus_tag="PK768_04430"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQA60247.1"
                     /translation="MVVRGAPAIGVAGAYGLVLAASEFIELPKEEFLEQMKESVNVIG
                     NSRPTAVNLMWAVKRMEAIIEENKEKSNVDIYKALLAEANTIEKEDLETNKAIARHGN
                     EIVPENATILTHCNTGALATVGYGTALGVIREAYYSGKNIRVFVDETRPRLQGAKLTA
                     WELVQEGIPATLIPDSVAPVLIRDGKIDLIIVGADRVASNGDTANKIGTYMLSQIAKD
                     FNVPFYIAAPISTIDFEIASGEEIVIEERDSMEITHINGVRIAPEGIEVYNPAFDVTP
                     NSNITGIITEKGIIRQPYSLNLAKLRQIQGC"
BASE COUNT         1815 a         1286 c         1357 g         2076 t
ORIGIN      
        1 ggagaaaatt gctcttggcg ttttatctag cttttatttt atctcttgca cagcgatgct
       61 gcttcttcat ccattattat tgttacatcg ggatgaagca tgagtaatga cgcgggaact
      121 tttgtagtta caacatcgct attaagaagt tttgccatga tttcggcttt attcttacca
      181 cttgccaaca gcagaatttt tttggctttc attatggagc caatacccat ggttatagct
      241 tttgtgggaa cttcttctgg cgaatcaaaa aaacgggaat ttgctttaat tgtatcttct
      301 gtaaggtcgg ttaggtgtgt tcctagaacc aattcctctg caggctcatt aaaacctata
      361 tgaccattta caccgattcc cagtagttgt aagtctatgc cgccatattt ttcgatttct
      421 tcatcgtact gtttgcagta agtttcgaca tctgatacca agccgtttgg tatgtggaca
      481 ttttcttttt ttatatttac atggttgaaa agattctcga acatgaaata attatagctc
      541 tggggattat cagaagcaag gccataatat tcgtctagat taaaggatac tgccttggaa
      601 aaatccaatc cttcttcctt atgcatccgg ataagctcct ggtatgtccc tataggagtg
      661 cttcctgtag caagcccaag aactaaatca ggtttttgtt gtattccatt agcaaaaatt
      721 tgagctgcct ttttactcat ggctgaataa tctttttcga taaagatttg catgctgttg
      781 cctcctgtca caaattagag tcttacacac atgattatac cattatgaaa aattgttgta
      841 tatattttat tccaagtgaa tataaaaatt ttaataatta gcttcgcctt cccttttgat
      901 atcttttttt gtctttttga aacatataaa gctatagtac ataaagtttc aagttattca
      961 ggtattccgt aacttgacat aatctaattg tctatgttag attgaaaaaa acagacagta
     1021 ccaaatgtgg aaagctacat ataaaagcac ttcaactgcc acagagcctt cgggtaaaaa
     1081 tcatatgcaa aatatcaaaa agaaaagggg gagttttttg ggcatacttg caagaaaatt
     1141 cacagaagta tcttattcag ttttgcctat aacggtttta gttattattt tacattttac
     1201 gttgattcct atagaaactg aaatgctcgc tagattcata ataggtgcta tattggttat
     1261 tatcggccta ggaatttttt tgtttggcgc agaaatcggt attgtcccta ttggaaacct
     1321 aatgggggaa accattgcaa aaacaaataa ttcatatctc gttggtatat tagggtttat
     1381 tcttgggttc ttaatcaccg ttgctgaacc tgatttgcaa atacttgccc gccaggtaaa
     1441 catggcatct ggtgggatta tatctgattt attaatactt attgtagttt caattggcgt
     1501 aggaattatg gttggaatag ggctgctgag aattctgtat gaaaagccgc tgaaccgtct
     1561 gcttaccctt gtttatttta ttattttttt attaggtata ttcacgtcac aggaattttt
     1621 agcaatttcc gtagatgctt caggtgctac taccggagcc atgacaaccc cttttatact
     1681 agctttaggt tacggagttt cccaattgaa aggtggtaag acatccgaag aggacagttt
     1741 cgggatggtc ggacttgcct ctgccggacc tatttttgcc gttatggcaa tgagtataat
     1801 ctccggttta aaagacatag cgggtacggc tgaagcattt gtgcccaata ccggaatact
     1861 gcagccgtat tttcatgcat tccccaaatt tctaaaggaa tccctgctga ccttatttcc
     1921 gctgtttgtc ctgtttatgg tatttgataa agctaagttt aagttaaaca agaaacataa
     1981 acatgcagta ttaaaagggc tattatatac atttatcggc cttactcttt tcttagtcgg
     2041 tgtcaatgct ggattcatgg aagttggaag ggtaatgggc caaggcatag caaaagatta
     2101 cttttggttg ctgccggtaa ttggcttttt cttgggcatg gtggtagtgt tagctgaacc
     2161 ggcagtctat gtgttaaccg agcaagttga ggatgttaca gccggccata taaaaaagaa
     2221 ggctatattg gccactttgt caattggaat tgcttttgca gttgtgtttt caatgatgag
     2281 aatcatgata ccaagtttga agctgtggca ttttttgttg cctggatttg cattcgccgc
     2341 ctttttatct tacagggtgc cgccaatttt tgtcggtatt gcttatgatt caggaggtgt
     2401 ggcatctggc cccatgactg ctacatttgt tttggccttt gctcaaggtg ctgccaatgc
     2461 aatcccgacg gcaaacgtta tggtggatgg ttttggtgtc attgctatgg tagctatgat
     2521 gccgcttgta gctatacaaa tactgggcct catatataaa atgaaggctg gagaagatgg
     2581 tcaaagtcaa tcatacgagg aggcgcaatc atcatgagcc caacgggcaa aaatcttgtt
     2641 ttgttttgtt gtatagtcga ctgtggcaaa ggaagtaaag tattaaaaat agcaaagaaa
     2701 gttggagctg taggcggagt tgtttttctt ggaaagggta cggtcagaga tgaattgctt
     2761 aagtttttag gcatcttgga tgtcagaagg gaaatatttt tatcagttat tgatgaagaa
     2821 ctggaagatg cattatataa tgaaatgaat aaaaagctcg ggctaaataa gcccaagcac
     2881 gggattgcat tttccatacc attaaagagt cttgtaaaaa tcgatgaaag agagtataaa
     2941 tccaaaaccg aggtgaaagg tgtggccaaa gtgagttatg aagccatatt tgtcattgta
     3001 gataaagggt ctttagatga tgtaatagat gctgcggaag cagcaggctc cacaggaggc
     3061 accgtgattc acggcagggg tttaggaaac catgaaaaag ccaggttgtt taatatcgaa
     3121 atcgagcctg aaaaagatat agtattgata ctgtcaaaag ttgataaaac tgaagatatt
     3181 gtcaatgcca ttagggagaa attgaatatc gatcatcccg gtacaggcat tatttttgta
     3241 cttgatgtaa aaaggaccgt agggttatat gaggataatt aattacctat tcatttcact
     3301 gtcagttcca gtttttttat gacttgcaat taactgctct atctctttaa aatcacaggc
     3361 aggcaggtcc ccaggcaccg tgttttttaa tgccgccata gcattgccaa actctagggc
     3421 cttttgaagg cagttaaatt tcactatgcc gaataagact ccggcaacgt atgcatctcc
     3481 actgccaatt ctgtcaacca cttcgatatc aatataagga tcttctttgt aaaatttgcc
     3541 ctctaatgca ttgtatatcc tcgagtccca ggtatgctta gttgggcttt ttgcctttct
     3601 ttcggtggtt gcgataattt ttattccata atcccttgca aaacccttca ttatatcctc
     3661 tagtgtgccc gtcctctgaa gcatgcgccg tgacgtttcc tcagagacaa ataaaatatc
     3721 cacgtccggg aaaattgaag ttatcacttt tctcgccgtt tcctcgtccc acaaagttgc
     3781 cctgtagttc acatcaagtg aaattgctgc tccatgcttt ttaaactttt tgatcatgtc
     3841 aataaccgtt tcccttacat ttacccctaa tgccaatgat ataccgctta catgaaaaat
     3901 cttgctggag ctgtacactg aagaatcaat ttcatctgca gatatagtcg taaaggatga
     3961 accactcctg tcatagacga cagttggctt tctaggaaag gccccacctt caaaataata
     4021 tattcccaat cgcttgcact cagattggtc atatattaca taatcgtcac ttacaccgtg
     4081 aaagcgtatc ttgtttttga taaatttccc tatctcgttg tcgggcagct tcgttatgat
     4141 cccagttcta agacctaaca tggagatacc cgataccacg ttaaattccg aaccacccgc
     4201 cctcttttca aagacttctc cctgggatat tctttcctta ttaggcggag aaaggcgcag
     4261 cataacctct ccaaatccta taacatcaaa ttccctattc ctgcactcat tatttattat
     4321 catcttttgc ctccttatat ttgaatattg ttgccttaat cgctttgact tcaaacctta
     4381 tagtctggag caattgatac aattcttttg taccttcctt ttagaagtgc taaattatat
     4441 tttcgttaat tgttccggtt tcccttcttt atatgaggta aaacaagtaa attattcgaa
     4501 ttgcacattt atcagcttta acaatataaa tttttgctgc ttcatacttt tatccacata
     4561 tttttttgcc gttagcacct aaatggctga acgtgaataa tatgttagca gaaaagccta
     4621 gagaattaat taaaaggggt gttataatgg cagatttaga aaagtttatc aaggacatgg
     4681 agaaagccag cgagaaactc gctgacatgc ttaataaaaa ggaagagccc attcccgagg
     4741 tggattcaga gttttccaca gaagtagagg aacttcaaga aggtcatgag attgctgaaa
     4801 ttgatgaaga tagccgggtc atagttaaaa atgcagatgt ggccgactgt ggctgtgatg
     4861 aaacagagca aaatcaagca actcctcaac cctgtaatcg aatagtgaga ttcaactgca
     4921 tagaaaacat tcctcgggga ttgaggttgg atagggttag agactttaga gtagtatatg
     4981 acccaacgga tttaaaggta tgcgttgaag aagctgaaat aagcgttacg ccgcctcccg
     5041 gttgtcctga cctgaccctt agcgtgtttg cagttcgagt tataggttgc ataccggtca
     5101 gcatcagcgt actggctttt gagggtagat gcggcgttaa ccttgttcca agaagagatc
     5161 gagatgatag agtagcatta tgctgccaca ccacggtttg cgttgataat gtaatatgct
     5221 ataggggcac cagggagcaa gcagaatttg cagcagctga aattcgaagg gagctgagct
     5281 ctgggaaaca atgcggcgta accgtaagcg aaacggaaga tgaagaagct gcggcccaat
     5341 tcataaaagg caggagagac ccctgtaatg ccgttcccct gctatttgcc atagggcgca
     5401 tctttagagt gcccgttggg caagataatg acgatccaaa gatactggcg ttttccggcg
     5461 cctttaggct ccccgcatgt ccagaagcaa atgccgcagg ttaagaacag atactttatt
     5521 tattataagc ctcctataat acggaggctt tttatattaa taaatccatg ttgaatatat
     5581 aagaagctaa tctttccata tggtttcatc agcatccctg gatttgcctt aacttggcaa
     5641 gattcaatga gtatggttgc cttattatgc ccttttccgt tataattccc gttatgttgg
     5701 agttaggagt gacatcaaag gcgggattgt aaacttcaat accctcgggg gcaattctaa
     5761 caccgttaat atgggtaatt tccatgctat ccctttcttc gattacgatt tcttctccac
     5821 ttgctatttc aaagtcaatg gtggaaatag gtgctgcaat ataaaaaggc acgttaaaat
     5881 ctttggctat ctgtgacagc atatatgtgc ctatcttatt tgcagtatcg ccgtttgagg
     5941 cgacgcggtc cgctccaacg atgattaaat cgattttacc gtcacgaatc aggacggggg
     6001 ccacgctgtc aggtataagc gtagcaggaa tcccctcctg gacaagctcc caggcagtaa
     6061 gctttgcccc ttgaagcctg ggtctcgtct catctacaaa aacccttata ttttttccgg
     6121 aataatatgc ttctctgatg acgcccaatg cagttccgta accgactgta gccagtgccc
     6181 ccgtgttgca atgggttaag atggtggcat tttcaggtac gatctcatta ccgtgtctcg
     6241 ctatagcttt gttggtctcc aggtcttctt tctcaatggt attggcttcg gccaatagag
     6301 ccttgtaaat atccacattg gatttttcct tattctcttc tataatcgct tccatgcgtt
     6361 tgacagccca cattaaattt acggctgttg ggcgagaatt gccaattaca ttgacgcttt
     6421 ctttcatttg ttcgagaaac tcttctttgg gaagttctat aaattcactc gctgccagga
     6481 ccaacccgta agcacctgct acaccaatgg ccggcgcccc acggactacc atat
//
LOCUS       DAONUO010000088         6441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9026_length_6441_cov_4.144535, whole genome shotgun sequence.
ACCESSION   DAONUO010000088 DAONUO010000000
VERSION     DAONUO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9026_length_6441_cov_4.144535"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PK768_04435"
     CDS             complement(<1..436)
                     /locus_tag="PK768_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQA60248.1"
                     /translation="MPKVAFYTLGCKVNQYESAAMAELFEKRGYEVVDFDAKADVYVI
                     NTCDVTNESGRKSRQVIRKAIRKNPSAKVAVVGCYAQLEPGQVSELPGVSVVIGTKDR
                     HKIVDLVEKAQASDEQVVAVGNIMKQRTFEDIAFKGHRLKTRA"
     gene            complement(439..1188)
                     /locus_tag="PK768_04440"
     CDS             complement(439..1188)
                     /locus_tag="PK768_04440"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQA60249.1"
                     /translation="MPRLFVFEKLAIGDEIVIAGEEAHHIIRVLRLGPGHSVSISDGK
                     SVESLGVISDIDIRDTKIKIRILDQNKSKETKPFITLLQALPKGEKFDWIIQKSTELG
                     VSKIIPVITQRTIVNILPSKLERRMERWNKIAIEAAKQSLRMDIPQIGELSTFDASLR
                     EVEKHHLSIIPWEQEKELSIRKALKSIDGTVTKVAVFIGPEGGFSPEEVKKAKEMGAV
                     SVSLGPRILRTETAAIAVCSILMYELGGMEG"
     gene            complement(1204..2151)
                     /gene="prmA"
                     /locus_tag="PK768_04445"
     CDS             complement(1204..2151)
                     /gene="prmA"
                     /locus_tag="PK768_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQA60250.1"
                     /translation="MNWVEIKVKTTTEAIEAVSNIFYEAGVAGVVIEDPKIYLRPSDN
                     EDWDYVEVPEDLDFEMVQVTGYLAEDSSLAERTQVIRERIRQLPSFGIDIGKGEVAIA
                     TISEADWGETWKKYYKPTHIGKNIVIKPSWEEYKAKTPDEIVIELDPGMAFGTGTHES
                     TVLCLEILENYMKRDFTVIDVGCGSGILSIACGKLGAKKVLAIDKDETAVKVATDNMK
                     RNNLGNSVKVVKGDKLHGVDFKADIIVANIIADVIIDITKDVGLYLKDGGVFISSGII
                     KDRKLSVIEAMERNGLDLIQQFEKGEWVALVSAQAPICS"
     gene            complement(2166..3314)
                     /gene="dnaJ"
                     /locus_tag="PK768_04450"
     CDS             complement(2166..3314)
                     /gene="dnaJ"
                     /locus_tag="PK768_04450"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQA60251.1"
                     /translation="MAKRDYYEVLGVSREATEDEIKKAYRKLARKYHPDVNKEDKDAA
                     EKFKEINEAYEVLKDPEKRARYDQFGHAGVGQGGFDAGDFGGFGGFGGFGDFGSDIFD
                     DIFDIFGGSFGRSRRRGPTRGADIRYDLEITLEEAAFGAEKEVEIFRMESCKNCNGTG
                     AKPGTMPKTCPSCGGSGQVRRVQNLGPMQFTNVTTCTNCGGRGTIIEEPCPKCHGRGQ
                     SKVGRKIKIKVPAGVDTGSQLRMSGEGEPGELGGPPGDLYVVIRVKPHKLFVRQGDDL
                     IYQAPISFVQATLGDEIDVPTLEEKVKLKIPEGTQPGTRFRLRSKGIPHLRGYGRGDL
                     IVQVEVVIPKKLNDKQKELLVKFAKISGEDIKPTSKGLFRKMKDAFGV"
     gene            complement(3340..5208)
                     /gene="dnaK"
                     /locus_tag="PK768_04455"
     CDS             complement(3340..5208)
                     /gene="dnaK"
                     /locus_tag="PK768_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQA60252.1"
                     /translation="MSKVIGIDLGTTNSVVAYMEGGQPAIINNAEGSRLTPSVVAFTK
                     EGERLIGQLAKRQAITNPERTIQSIKRHMGTDHKVTIDDKTFTPQEISAMILQKLKQD
                     AENYLGEKITKAVITVPAYFSDSQRQATKDAGTIAGLEVLRIINEPTAAALAYGLDKE
                     DDQTILVFDLGGGTFDVSILELGDGVFEVKATSGNNRLGGDDFDQRIVDYVADEFKKE
                     HGIDLRKDRMALQRLREAAEKAKIELSSMASTNINLPFITADASGPKHLDMTLTRAKF
                     EELTRDLVEATMGPTRRALSDAGLEPHEIDKVILVGGSTRIPAVQEAIKNLTGKEPHK
                     GVNPDEVVAMGAAIQAGVLAGEVHDVVLLDVTPLSLGIETLGGVFTKLIERNTTIPTS
                     KSQIFTTAADGQTAVDIHVLQGERPMAADNITLGRFQLTGIPPAPRGLPQIQVTFDID
                     ANGIVNVSAKDLGTGKEQKITITSSTGLKEEEIERMIKDAERFAEEDKKKKEKIEAKN
                     KADSLIYQTEKMLSDLKDKISKDDEDKIKREVENVKAALESDDTDRIQKATDDLTKVF
                     YDISSRLYQQSAQANPGDQNFSGTDSSAEQAGSSNSKDEKVVDADYKVVDDDEDKK"
     gene            complement(5221..5886)
                     /gene="grpE"
                     /locus_tag="PK768_04460"
     CDS             complement(5221..5886)
                     /gene="grpE"
                     /locus_tag="PK768_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQA60253.1"
                     /translation="MTIKPGEDLSCRQNGDECKEANGQNAGKNESIEDREQVEAQEGK
                     TYQHELQQETDLRKDNGEQTESQDINSLNELLEDKQREIEEYRNRWLRSQADFDNYKK
                     RMQREIQDINLYAGEQLVKDILPVIDNFERALNSVKDEDDAFYKGVKLIYQQIVDVLN
                     KHEIKEIEALGKPFDPNFHEAVMIIESEELEPDTVAEVLLKGYTYHSKVIRPSMVKVV
                     KNQ"
     gene            complement(5910..>6441)
                     /locus_tag="PK768_04465"
     CDS             complement(5910..>6441)
                     /locus_tag="PK768_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HQA60254.1"
                     /translation="ATVLVGAATDEIVGERERIAKDAASSLQAAVLYGMVPGGGAIEI
                     AISRELEKERNKTKGMAAYGIDCVISALKKPLAQIVYNAGYNPLEKVEEAIYSQSEAN
                     KNSIGINCENGKAEDMVELGVVDPAQVKAYALKAAGEVSEAILRINTIIKKRDNMPAG
                     QGPGTESPMSMSEMDF"
BASE COUNT         1503 a         1651 c         1142 g         2145 t
ORIGIN      
        1 aagccctggt ctttagacgg tggcctttaa aggctatatc ctcaaaggtc ctctgtttca
       61 ttatgtttcc tactgctaca acttgttcgt cacttgcttg ggccttctcc accaaatcta
      121 ctattttatg gcggtccttt gtccctatca cgacggaaac accgggcagc tcagaaacct
      181 gacccggttc cagctgagcg taacatccca ctaccgctac ttttgccgat ggattttttc
      241 taatcgcctt tcgaataacc tgccttgact ttcttccgct ttcatttgtt acatcacagg
      301 tgtttattac atatacgtcg gctttggcat caaaatctac tacctcgtaa cctctcttct
      361 caaacaactc agccattgcc gccgattcat attgatttac cttacaacca agtgtataaa
      421 aggccacttt tggcattatt atccttccat acctccaagt tcatacatta atatgctgca
      481 tactgcaatt gctgcagttt cagtcctcaa aatccttggg cccagactga cggaaacagc
      541 ccccatttct ttcgcttttt taacttcttc aggcgaaaat ccaccttcgg ggccaataaa
      601 gacagctact tttgtgaccg ttccatcaat gctctttaat gcttttctta ttgatagttc
      661 cttttcctgc tcccagggga taatactcaa atgatgcttc tcgacttccc tcagtgatgc
      721 atcaaaagta gaaagttcgc caatttgtgg tatgtccatc cgcaaggatt gttttgcagc
      781 ttcaattgcg attttattcc agcgctccat gcggcgctca agctttgatg gcaaaatatt
      841 tactatagtc ctctgggtaa taaccggaat aattttactt actcctaatt ccgtactctt
      901 ttgtataatc caatcaaatt tttctccctt aggcaaagct tgcagcagag taataaaggg
      961 ttttgtttcc ttggacttat tttgatccaa aattcttatc tttatttttg tatcccgtat
     1021 gtcaatatcc gaaatgactc caaggctctc tacacttttt ccgtcggata tacttaccga
     1081 atgaccggga cctaatctta gaaccctgat aatgtgatga gcttcttcac ctgcaataac
     1141 aatctcatct ccaattgcaa gcttttcaaa gacaaaaagt ctaggcataa aaatttcccc
     1201 ccatcaacta cagatggggg cctgtgcgga caccaatgcg acccactcgc ctttctcaaa
     1261 ttgctggatc aaatccaatc cgttcctttc cattgcttca attactgaca actttcggtc
     1321 tttaataatt cccgatgaaa taaaaacacc gccgtccttt aagtacagtc ccacatcttt
     1381 ggtaatgtct attattacat ccgctatgat attggcaact attatatctg ctttaaagtc
     1441 aacaccatga agtttgtcac ctttgacaac tttaacagaa ttgcccagat tgtttcgttt
     1501 catgttatct gtggcaactt taacggcagt ttcatcttta tctatagcca atactttttt
     1561 tgcaccaagc ttgccgcagg caattgaaag tattcccgaa ccgcagccaa catctattac
     1621 ggtaaaatcc cttttcatat agttttccaa tatttcaagg cagagcaccg tactttcatg
     1681 ggtgcctgtt ccaaaggcca tgccgggatc caattctata acaatctcat caggagtttt
     1741 agccttgtat tcttcccagg agggcttaat cacgatattt ttgccaatgt gggtaggttt
     1801 atagtacttt ttccaagttt cgccccaatc cgcctcagaa attgtagcga tagctacttc
     1861 tcctttgccg atatcgatac caaagctggg aagttgccta attcgctctc ttataacctg
     1921 ggtcctctcg gctagactac tgtcttccgc caggtaaccg gtcacctgga ccatttcaaa
     1981 atccaaatct tcagggacct ctacataatc ccaatcctca ttgtcagatg gtcgcaaata
     2041 tattttggga tcttcaatta caactcccgc aactcccgct tcataaaaaa tgttggatac
     2101 tgcttcaatg gcttcggtag tagtttttac ctttatttct acccagttca ttaaaatcct
     2161 cccggctata caccaaaggc atctttcatc tttcgaaaaa gacctttaga tgtaggtttt
     2221 atatcctctc cacttatttt agcaaattta accagcaact ctttttgttt gtcattcagt
     2281 ttctttggaa taactacctc tacctgtaca atgaggtctc ctcgcccgta tcccctcaaa
     2341 tgtggtatac ctttagacct caagcgaaat ctcgttcccg gctgtgttcc ctcgggtatt
     2401 ttcagtttta ccttttcttc cagtgtgggg acgtctatct catcacccag ggttgcctga
     2461 acaaaggata tgggagcctg gtagattaag tcatcgccct gccttacaaa caatttgtga
     2521 ggtttcaccc ggatgaccac atacaaatct cccggcgggc ctcctagctc tccgggttct
     2581 ccttccccac tcatccgtag ttgggaaccc gtatcaacac cggctggaac ctttatcttt
     2641 attttgcgcc ctactttgct ctgtcctctg ccgtgacact ttggacaagg ctcctctatt
     2701 attgtacctc taccgccgca atttgtgcaa gtagtgacgt tggtaaactg catgggaccg
     2761 agattttgta ctctcctcac ctgaccgcta ccgccgcaag aaggacatgt cttaggcatg
     2821 gtaccgggct tggcaccggt accattgcaa tttttgcagc tttccatgcg gaatatctca
     2881 acctcttttt ctgcaccaaa ggcagcttct tccaaggtta tttctaagtc gtatcttata
     2941 tcagcgccac gggtcgggcc ccttctcctg ctccggccaa agcttcctcc gaagatatca
     3001 aaaatatcgt caaagatatc gctgccaaaa tccccaaagc caccaaaacc gccaaagcct
     3061 ccaaagtcac cggcatcaaa gccgccctgc ccaacacctg catggccaaa ctgatcgtat
     3121 cgtgctcgct tttccggatc ttttaagacc tcatatgcct cgttgatttc tttaaatttt
     3181 tctgctgcat ctttgtcttc tttgtttacg tccggatgat attttctggc aagtttcctg
     3241 tatgcctttt ttatttcatc ttcggtcgct tctctgctaa cgcctaatac ctcgtaatag
     3301 tctctctttg ccaatcggca tcactccatc caggggccct tattttttat cttcatcatc
     3361 atccacaact ttgtaatctg catcgactac tttttcatct ttactgtttg aactaccggc
     3421 ttgttcggcg ctggaatctg ttcctgaaaa gttttgatct ccgggatttg cctgggcgct
     3481 ttgctggtaa agtctcgaag aaatatcgta aaataccttt gtcaagtcat cggtagcttt
     3541 ttgaattcta tcggtgtcat cagattccaa agctgctttt acattttcaa cttctctttt
     3601 gattttgtct tcatcatctt tgcttatttt atccttcaaa tcggataaca tcttttcagt
     3661 ttgatagatt agtgaatcag ccttgttttt ggcctcaatt ttttcctttt tctttttgtc
     3721 ttcttcggcg aacctttcag cgtcttttat catgcgctca atttcctctt cttttaagcc
     3781 tgtagaagag gttatagtaa tcttctgctc ttttcctgtg cccaggtcct ttgcagacac
     3841 atttacaatt ccattggcat ctatgtcaaa agtaacttga atctggggta atcctcttgg
     3901 agcaggcggt attcctgtaa gctggaatct gccgagagtt atattgtcgg cagccatggg
     3961 tctttcgccc tgcaacacat gtatgtcaac tgctgtctga ccatctgctg ctgtggtaaa
     4021 aatctggctc ttagaagtgg gaattgtcgt gtttctctca ataagttttg taaatactcc
     4081 acctaaggtt tcaattccta gggacagcgg tgttacatcg agcagtacga catcgtgtac
     4141 ctcgcctgcc agtacaccag cctgtatagc ggcccccata gccacgactt catcaggatt
     4201 tacacccttg tgaggctctt tacctgtcag atttttaatg gcctcctgca ctgccggaat
     4261 ccgtgtagaa cctcctacta atataacttt gtcgatctca tggggctcta aaccggcatc
     4321 ggataatgca cgccttgttg gacccatagt tgcttcgact aaatcccggg tcaactcttc
     4381 aaattttgcc cttgtcagtg tcatgtcaag gtgtttggga ccgctggcat cagcagtgat
     4441 gaagggcaga tttatatttg tagatgccat acttgaaagc tctatcttcg ccttttctgc
     4501 agcctctctc aatctttgaa gggccatcct atccttgcgc agatcaatgc cgtgttcctt
     4561 tttaaactca tcagccacgt aatcgacaat gcgttgatca aaatcatctc cgccaagtct
     4621 attgttgccg ctggtggcct tcacttcaaa gaccccatca cccagttcta ggatggaaac
     4681 gtcaaaagtt ccgccaccta ggtcaaatac caaaatagtt tggtcatcct ctttatccaa
     4741 gccgtaagct aaggctgcag ctgtaggctc attgataatc cttagcactt caagacctgc
     4801 tatagtccct gcatctttag tggcctgtct ttgactgtca gaaaaatatg cgggcaccgt
     4861 tatgacggcc ttagtgattt tttcccctaa ataattctcg gcgtcctgct ttagcttttg
     4921 caatatcatg gcagatattt cctgtggcgt gaaggtttta tcatcaattg ttaccttgtg
     4981 atccgtcccc atgtgccttt ttatggactg tatagtcctc tcggggtttg ttattgcctg
     5041 acgcttggcc aattggccga tcaggcgttc tccttcttta gtaaaagcca ctactgaagg
     5101 cgtcaatctt gaaccttcag cattatttat aatggccggc tggccacctt ccatgtatgc
     5161 cacaacggag tttgttgtac ctaagtcaat tcctatgacc ttactcatac aaaatcacct
     5221 ctattgattt ttaacaactt taaccatgct cggtctaatt actttagaat gataagtata
     5281 accttttagc aaaacttctg ccaccgtatc tggttcaagt tcctcacttt caatgatcat
     5341 aactgcttca tgaaagttgg gatcaaatgg tttgccaagc gcttctattt cctttatttc
     5401 atgtttatta agtacatcaa caatctgctg gtaaatcaac ttgacgccct tgtaaaaggc
     5461 gtcatcctca tcttttaccg agtttaaagc cctttcgaaa ttatcaataa caggcaagat
     5521 atcttttacc agctgctcgc cggcatataa gttgatgtct tgaatttccc tctgcattct
     5581 tttcttgtaa ttgtcaaaat cagcttggga tcttagccac ctgttcctat attcttcaat
     5641 ttctctttgc ttatcctcaa gaagttcgtt gagagaattg atatcttgag attccgtttg
     5701 ttcaccattg tctttacgta aatcagtctc ctgctgcaat tcatgttggt aagtttttcc
     5761 ttcctgtgct tctacttgct ccctatcttc tatggactca tttttccctg cgttttgacc
     5821 atttgcctct ttgcattcat caccattttg tctgcaggat aaatcttctc ctggcttaat
     5881 agtcaatggt ctatatctcc tttcattaat taaaaatcca tttcactcat actcatcgga
     5941 gattccgtac ccggaccttg tcctgcaggc atattgtcgc gtttctttat tatagtattg
     6001 attcttaaga ttgcttcaga gacttctcct gcagctttta aggcataagc tttcacttga
     6061 gcggggtcca ctactccaag ctctaccatg tcttcagctt tgccgttttc acagttaatg
     6121 ccgatggagt ttttgttcgc ttcagactgg ctgtatatgg cttcttccac tttttccaga
     6181 ggattgtagc cggcattgta tacaatctgg gccaaaggtt tctttaaggc agaaatgacg
     6241 caatctatac cgtaagcagc catgcctttg gtcttattgc gctctttttc cagctctcgg
     6301 gaaatagcaa tttcaatggc tcccccaccg ggaaccatac cgtaaaggac tgctgcttga
     6361 agggaagatg ccgcatcttt tgctatgcgt tccctttctc ccactatttc atcggtagca
     6421 gctccgacca ataccgtggc c
//
LOCUS       DAONUO010000089         6369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9147_length_6369_cov_4.079823, whole genome shotgun sequence.
ACCESSION   DAONUO010000089 DAONUO010000000
VERSION     DAONUO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9147_length_6369_cov_4.079823"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="PK768_04470"
     CDS             <1..1417
                     /locus_tag="PK768_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQA60255.1"
                     /translation="NFDEIMAECQEENRPELLARIDKTITQWVYNHNGLLKKYDDDKY
                     LVLLNLRDFQRAEEAKFNILDAMREIKVSQVMTPTLSIGAAYGEATLTQSSKLAQTAL
                     ELCLGRGGDQVVVKAFGKTYFYGGKSKAVEKYSRVRARVISHALRNLIEESDMVMVLG
                     HLFLDMDALGAGAGIVSAVKSLNKPGYIILTPEQSPSVDALIELLLGDEKTRKDFIGE
                     KEALEKITKKTLLVVLDTHKPSLVMSQKVLEKVEKVVVIDHHRRGEEFIDKAMLVYLE
                     PYASSTSEMVTELIQYMGEDIKISPLAATAMLSGIAVDTRNFAFKTGVRTFEAASFLR
                     RAGADPIIVYKLFQEEADTVHQRAKVIKRAEEFVDHVVISYYDEEPANPTLAAAQAAN
                     SLIGLKGIYASFVLVPMGDRISISGRSLGDINVQRILEKLGGGGHMTVAGAQLSGVSM
                     PEALEMVKQAVIDYLKEGEAS"
     gene            1414..1860
                     /gene="rplI"
                     /locus_tag="PK768_04475"
     CDS             1414..1860
                     /gene="rplI"
                     /locus_tag="PK768_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQA60256.1"
                     /translation="MKVILLEDVKKLGKKGDLIEVADGYARNYLLPRNLAREATEGGI
                     KQLKQEKAALENKKRKERQQAQALAAKLSEMTVTLKVKSGEQGKLFGSVTSKDISEAL
                     KEQHDIEVDRRKIELQEPIKSLGNYEVDIKLAPGIDAKLKVKIVEG"
     gene            1865..3205
                     /gene="dnaB"
                     /locus_tag="PK768_04480"
     CDS             1865..3205
                     /gene="dnaB"
                     /locus_tag="PK768_04480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQA60257.1"
                     /translation="MDNLKVPPYNLEAEQSVLGSMLLSQDAVIVATEQLQPEDFYMES
                     HRRIFDVISRLYENREPIDLITVTEALRSNKILEQVGGAVYLTTLTETVPTASNIAYY
                     CRIVEEKAILRRLISTTSGILSMAYDPEYQVEELLDEAERKIFEIAQKRKVENFYHIK
                     DILFDTFERIEQLYNTKGGITGVPTGFPDLDEKTSGFQPSDLILIAARPSMGKTAFAL
                     NIAQNAAIRYKVPVAIFSLEMSKEQLVQRMLCAESNVDSHKLRTGRLDEEDWPKLARA
                     MGPLSEAPIYIDDTPGISSLELRAKARRLKAEKGLGLIIIDYLQLMSGRGNSENRQQE
                     ISEISRSLKALARELNVPVVALSQLSRAPEMRSDHRPILSDLRESGSQEQDSDVVAFL
                     YRDDYYNPDTDKKNIAEVIIAKQRNGPTGTVELVWLPRFTKFASLEKYRQSQVG"
     gene            3290..3973
                     /locus_tag="PK768_04485"
     CDS             3290..3973
                     /locus_tag="PK768_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQA60258.1"
                     /translation="MSETDIIRKIPIFSDLEPEELKEVSNIYIPRKYKKGQIIFFEGD
                     PGEAVYFVKEGKIKIYKSDPEGREYILHIFGPGNIFAETVLLGGDPYPANAETVEDSV
                     VGVIKNSDLEKLLKKNTDIAFKIIKVLSKRLRDSQENIKNLVFRDTYDRTACALHKMS
                     LEHGIKTPQGIEVEMPITRSELASIVGTSRETVTRMLSEMKRKGIIDMDRQKIIIKNE
                     RELMVCVRD"
     gene            complement(4154..5062)
                     /locus_tag="PK768_04490"
     CDS             complement(4154..5062)
                     /locus_tag="PK768_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQA60259.1"
                     /translation="MNLKEQQYICTLADTGNMTEAAKKLGISQPALSLFVSNLENSLG
                     VKLFERVGKRLILTYVGELYTQKARQMLNLKDLFDAELPGIIKGYKGRLRIGMQSFRS
                     PHITPALLKRFQNDYPNVEIVLHEGNYRFLEGLLLDNQLDLFFCNCPHRNQKIQYISL
                     RREPILLAAPTNHPKLSQAQRVTGSKYPWIDLAHFRNERFILQYEGQSLRASVDEILE
                     DTGIKPGKLFCVRSIGTSLRMTAEGMGVSFCNESYTSYFDFNKKPAFFLVGNHIHTTE
                     FVAAYAKGRTLPGYANHLINILRDLL"
     gene            5348..>6369
                     /locus_tag="PK768_04495"
     CDS             5348..>6369
                     /locus_tag="PK768_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQA60260.1"
                     /translation="MIRKNLKKEIGKHIEEISEALCEIADKIFDYAEPGFEEHKSSAL
                     LCDWLQQRGFNVERGICGLDTAFRGSYEFGTNGPSIGLLCEYDALPIGHACGHHLQGP
                     VMLAVADALKSHVFDRPFKLVVYGTPAEEGLQGKAHMLRNGCFKDVDLALMVHAADRT
                     TVDVRSLAGAKLNVKFKGIAAHHSIAPEASRDALDGLILAFQGIEFLRGHIKDDVRIY
                     YGIKDCIGMPGNKEQTNAEAYVIFRTYHNGDVGELEQRITDIFKGAALMTGTEVEIRK
                     SHEMVGKLPSHILNKLILDNAEALSAPQILKPHREKTGSTDFAFVSHLIPSAVCRVAF
                     VPEGTPP"
BASE COUNT         1872 a         1380 c         1639 g         1478 t
ORIGIN      
        1 caacttcgat gaaattatgg cggaatgcca ggaggaaaac aggcccgagc tcttggccag
       61 gattgataaa accattaccc aatgggtata caaccacaat gggcttttaa aaaaatacga
      121 tgacgacaag tacctggtac tgctaaatct tagagatttt caaagggccg aggaagccaa
      181 gtttaacata ttggatgcca tgcgggaaat aaaagttagc caggtaatga cgccaacttt
      241 gagcatcggg gcagcttacg gagaagctac cctcactcaa tctagcaagc tcgcccaaac
      301 tgcattggag ctttgcttag gccgcggcgg cgaccaggtg gtggttaaag ctttcggcaa
      361 gacgtatttt tacggcggca aatccaaggc agtggaaaaa tacagccgcg tgagagcccg
      421 cgtcatatcc catgccctga gaaacttaat cgaagagtca gacatggtaa tggtgctggg
      481 gcacctcttt cttgacatgg atgccctggg cgcgggagcg ggaatagtaa gcgcagtaaa
      541 gagcttaaac aaacccgggt acatcatact cacaccggag cagagcccat cggtggatgc
      601 cctcatagaa ctgctgctgg gcgatgaaaa gactagaaag gacttcatcg gagagaagga
      661 agccttggag aagatcacga aaaaaaccct gctcgtggta ttggatactc acaaaccctc
      721 ccttgtgatg tcgcaaaagg tcctggaaaa ggtagaaaag gtcgtggtca tcgatcacca
      781 ccgccgggga gaggagttta tagacaaggc catgctggtc tatctagaac cctacgcttc
      841 ctctaccagc gaaatggtga ccgaactcat acagtatatg ggagaagaca tcaagatatc
      901 gcccctggcg gctacagcca tgctatccgg catagcagta gacacccgca attttgcctt
      961 taagaccggc gttcggactt ttgaagcggc gtcctttttg aggcgggcgg gggctgaccc
     1021 gattatagta tataagctgt tccaggaaga ggcggatacc gtgcaccagc gggcgaaggt
     1081 aataaagagg gccgaagagt ttgtagacca tgtggtaata tcctattacg atgaggagcc
     1141 tgccaatccc accctggcag ctgcccaggc agccaacagc ttaataggtt taaagggcat
     1201 ctatgcttcc tttgtcctgg tgcccatggg tgataggata tccataagcg gtcgctcttt
     1261 gggcgatata aatgtacagc ggatcttaga aaaactgggt ggcggaggtc acatgaccgt
     1321 ggccggcgcc caactttccg gggtaagcat gccggaggct ttggaaatgg tgaaacaagc
     1381 ggtaattgat tatctgaaag agggtgaagc atcgtgaagg taatattgtt ggaagatgtg
     1441 aaaaagctcg gcaagaaagg cgatctgata gaagtggctg acggctatgc cagaaattat
     1501 cttcttccta gaaatttagc cagggaagca actgaaggag gcataaagca gctgaaacag
     1561 gaaaaggctg cccttgaaaa caaaaagaga aaggaaaggc agcaggccca agcccttgct
     1621 gcgaagcttt ctgagatgac ggttaccctt aaggtaaagt cgggtgaaca gggaaagcta
     1681 ttcggctcgg ttacttcaaa agacatatcg gaagctctaa aagagcaaca cgatattgaa
     1741 gtggacagac gcaagataga actgcaggag cctataaaat ccttaggcaa ttacgaggtt
     1801 gacataaaac ttgccccggg catagatgca aagctcaagg taaaaatagt tgaagggtga
     1861 ttttatggac aatcttaaag tgcctcccta taacctagaa gctgagcaat cagtgctagg
     1921 ttccatgctc ctgtcccaag atgccgtaat cgtagccacg gaacaattgc agccggagga
     1981 tttctacatg gaatcgcacc ggcgaatttt tgatgttatt tcaaggcttt acgaaaatcg
     2041 agaacccatc gacctgatta cagtaacgga ggccttaagg tccaataaaa tattggaaca
     2101 ggtaggaggt gccgtgtacc ttactaccct tacggaaacg gtgcctaccg catcaaatat
     2161 tgcgtactac tgcagaattg tagaggaaaa ggcgatttta aggcgcctga taagtactac
     2221 ttcaggtata ctgtcaatgg cctatgatcc cgaatatcag gttgaagagc ttttggatga
     2281 agcggagcgg aaaattttcg agattgccca aaagcgcaag gtggagaatt tctatcatat
     2341 aaaggatatc ttgtttgata cctttgaacg cattgaacag ctctacaaca ccaagggcgg
     2401 gataactggc gtgccaacgg gctttccgga cctggatgaa aagacatcgg gttttcagcc
     2461 ttccgacctc atcttaatcg ctgctaggcc gagcatgggc aagacggcct ttgccctgaa
     2521 catagcccag aacgccgcca ttcgttacaa agtacctgta gctatattca gcctggaaat
     2581 gtccaaggag cagttggtcc agcggatgct atgcgccgaa tcaaatgtcg acagccacaa
     2641 gctgaggacc ggcaggctcg atgaagagga ctggccgaag ctggcgcggg ccatgggccc
     2701 actgtcggag gcacccatat acatagacga tacgccgggg atatcctccc tggaactccg
     2761 tgcgaaggca aggcgcctta aggccgaaaa gggcctgggc cttataatca tagattacct
     2821 acagctgatg tcaggcaggg gcaattcgga aaaccggcag caggagattt cggaaatctc
     2881 caggtcccta aaagccctgg ccagggaact gaacgtgccc gttgtagcgc tgtcgcagct
     2941 ttccagggcc cccgaaatga ggtcagacca caggccgatc ttgagcgatc ttagggaatc
     3001 cggcagccag gagcaggatt ccgacgtggt tgccttttta tacagggatg actactacaa
     3061 tcccgatacc gataagaaaa acattgccga agtcataatt gcaaaacaga gaaacggtcc
     3121 taccggcaca gtcgagctcg tatggctccc gagatttacg aaatttgcaa gcctggaaaa
     3181 ataccggcaa agtcaggtgg gctagctgac ttgccgataa aggtggggtt tttgcagagg
     3241 tagatttcct attttgaatt taagggggtt gaaggctcag ccgtagtata tgagtgagac
     3301 ggacataatc agaaagatac caatattttc ggaccttgaa cccgaggaac taaaagaggt
     3361 cagcaatatt tatattccga gaaaatataa gaaaggccag ataattttct tcgaaggtga
     3421 tcccggtgag gcggtatact ttgtaaaaga ggggaagata aagatctaca aatccgaccc
     3481 tgaaggccgc gagtacatac tccacatatt cggacccggc aatatttttg cagagacagt
     3541 cctcctcggt ggggatccat atcctgccaa tgcggaaacc gttgaagact cggtggtggg
     3601 agttataaaa aacagcgacc tggagaagct ccttaagaaa aacaccgaca ttgcctttaa
     3661 aatcatcaaa gtcctgtcaa aacgactccg ggattcgcag gagaacataa agaacttagt
     3721 tttcagggac acttatgaca gaactgcctg tgctcttcac aagatgtcct tggagcacgg
     3781 cataaagacc ccccagggaa ttgaagtaga aatgcccatt accaggtcag agctagcatc
     3841 tatcgttggc acttcccgcg aaaccgtaac gcggatgctc agcgaaatga agcgaaaggg
     3901 cattatcgac atggacaggc aaaaaatcat aataaagaat gagcgggagc tcatggtctg
     3961 cgtgagagat taaaatatcc cccttcagcg acgctgaagg gggatatttg ttaatcaaca
     4021 gttatgataa ttaattgtat caagagcgca aaagtaatca gtttacggct ttttatacta
     4081 atcaaggctt gtagttttaa agcgattata aattaaatgg cgcctaaaag cataaattta
     4141 taaaaggaaa gcactataaa agatccctca gtatatttat aaggtggttg gcataaccgg
     4201 gaagggtccg gcctttggca tatgcggcca caaactccgt cgtgtgaatg tggttgccca
     4261 ccaggaaaaa ggcaggcttt ttgttaaagt caaagtacga ggtgtaactt tcattgcaaa
     4321 aggatacgcc catcccttcc gcagtcattc gaagcgaggt gccgatgctg cggacgcaaa
     4381 aaagttttcc aggttttatg ccggtatctt ccaggatctc atcgacagaa gccctgaggc
     4441 tttgcccttc atattgcagg ataaagcgct cgtttctaaa gtgtgcaaga tctatccagg
     4501 gatacttgct tcccgtgacg cgctgagcct gtgaaagctt cggatgattg gtgggtgcgg
     4561 caagcagtat tggctccctg cgcagggaaa tgtactgaat tttctggttg cggtggggac
     4621 agttgcagaa gaacaaatcg agttgattgt ccaagagcaa accttccaaa aatcgatagt
     4681 tgccctcgtg aaggactatt tccacattgg gatagtcgtt ttggaacctt ttaagcaggg
     4741 caggggttat gtgaggagag cgaaatgact gcattccgat gcgaaggcgc cctttataac
     4801 ccttgataat tcccggcagt tctgcatcaa ataggtcctt gagatttagc atttggcggg
     4861 ccttttgtgt atatagctcg cccacataag ttaaaatcag gcgcttgcct acgcgttcaa
     4921 agagcttgac acctagggaa ttttcaaggt tgctgacaaa aaggctcagg gcaggctggg
     4981 aaatgcccaa cttttttgca gcctcggtca tgtttccggt atcggccaaa gtacagatgt
     5041 actgttgttc tttcaagttc acggcagcac ccccaaaaaa attatttccc aaagaggaga
     5101 aaacattaga aacatttatc aatatcaata ttatcataat attttatacc gattgtgtca
     5161 atatttcgac aaaatagtaa acctaataat atcttaaatc agtgatttaa caatggttat
     5221 aacggacaat taatcaataa gaatagatta tgaaagttat aatttacatt aattttacaa
     5281 atatagtttt atttagtata ctataaatta acacaacttg taaaactatc agaaaatggg
     5341 gagacgtatg attaggaaaa atttaaaaaa agaaataggc aagcatattg aagagatatc
     5401 ggaagctttg tgcgagatag cggacaagat ttttgattat gcagagccgg gttttgaaga
     5461 acacaagtcg agtgccctcc tctgcgattg gctgcagcag cgaggcttta acgtggagcg
     5521 tgggatttgc ggcttggaca ctgcctttcg aggaagctat gaatttggaa caaatgggcc
     5581 gtcaattgga cttctttgcg aatatgacgc gctaccgatc ggacacgcct gcggccatca
     5641 ccttcaggga cccgtgatgc tggccgttgc agatgcatta aaaagccatg tttttgacag
     5701 gcccttcaag cttgtagttt acggcactcc tgccgaagag ggcctgcagg gcaaggcaca
     5761 catgctgaga aatggttgtt ttaaagacgt cgaccttgca ttgatggtgc acgcagcgga
     5821 ccgcaccacg gttgatgtaa ggtctctggc gggggccaag ctcaatgtca agtttaaagg
     5881 catagcagcc catcactcaa tagctcccga ggcatcccgt gatgccctgg atgggctgat
     5941 actggcattc cagggtatcg aatttttgcg gggccatata aaagatgatg tgcggatata
     6001 ttacggaata aaagattgta tcggcatgcc gggcaacaaa gagcagacaa atgcggaggc
     6061 ttatgtgatt tttaggacat accataatgg cgatgtaggg gaattggagc aaagaattac
     6121 ggatatattc aaaggtgcag ccctaatgac ggggacagaa gtcgagatcc gaaaatccca
     6181 tgaaatggtt ggcaagttac cttctcacat cctcaacaag ctcattttag acaatgcgga
     6241 ggccttgtct gccccgcaga ttttaaaacc ccacagggaa aaaacggggt ccacggattt
     6301 tgcctttgtc agccacctaa tccccagtgc agtctgtcgc gttgccttcg tgcctgaggg
     6361 gacgccacc
//
LOCUS       DAONUO010000090         6349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9184_length_6349_cov_2.986813, whole genome shotgun sequence.
ACCESSION   DAONUO010000090 DAONUO010000000
VERSION     DAONUO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9184_length_6349_cov_2.986813"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1718
                     /locus_tag="PK768_04500"
     CDS             198..1718
                     /locus_tag="PK768_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQA60261.1"
                     /translation="MATPNDKLAESLQVLQELQNGGVIAIRSNQLSRTHRERLLKQGF
                     LKEVMKGWYIPAGPDELPGDSTSWYASFWHFAAEYLHERFGNNWSISPEQSVMLHAAN
                     YAVPTQLYIRASGARNHITELIHGTSLLEIRAALPNEADLIEKDGLRLFSLSSGLINC
                     NENFYTRNPTDARTALLMIRDSSEVLGMLLDGSHSVVAGRLAGAFRNVGRTRMADEIL
                     DSMKAAGFTVRETDPFEHKTPVIIPQRERSPYIKGIRLMWATMREVVLQEFPVAPGMP
                     PDIEAYIKEVDDNYVNDAYNSLSIEGYRVTPELIEKVRSGKWQPDLNEDDRNQSNALA
                     ARGYWQAFQAVKESVIKILNGNNAGNVTDKDHGTWFRELLGPSVTAGILKASDLAGYR
                     NMPVFIRRSHHTPPNVDAVRDLMPVLFEMLAEEKEAAVRVVLGHFIFVYIHPYRDGNG
                     RIGRFLMNTMMASGGYPWTVVPLARRDEYMAALESASVGHDIRPFAQFLASLLPNE"
     gene            2420..3640
                     /locus_tag="PK768_04505"
     CDS             2420..3640
                     /locus_tag="PK768_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQA60262.1"
                     /translation="MESEKVSVTFFLDASRPNSEGKCLVKLNIYCRPDKQRYPTIFHL
                     TKEEWKKINGQKLKDEELKIVRNKLNALKDKVERIIKGLTPFSFVAFEDVYLRNKTNI
                     AGTSVKTWFENYIVSLEVQERIGSAMAYTTAMNSLIGYRKNLHLQDITPTFLVTYEHY
                     MIERNASLSTVGSYLRQLRAIINLAIRKKVVSADNYPFKEYQIPSTRNVKKALPNTEL
                     KKLLEYVPEDQEERKALDYWILMYLLSGINPADVIRLKPANIDNQYLNFFRFKTRYTK
                     KKDLRPVKVGIPKRAMEIIERRKNIDPDNEYLFPILHQELSARQAKFKGQDFAKWIND
                     KMEKIRKDLKISQKVRNNEARHTYATVMKRKNVGIVFIKETLGHSSLNTTELYLDSFP
                     DETKVEYANMLEQL"
     gene            complement(3882..5276)
                     /locus_tag="PK768_04510"
     CDS             complement(3882..5276)
                     /locus_tag="PK768_04510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HQA60263.1"
                     /translation="MRKLYLIILLLTTIIGSQAQVLFDQGKLTPPSSGVIPNFVIIGN
                     WNKTNITYFFSNGTADIAGNDERAAVVQGMLLWSAFTPLTFTEASNAASADIVISWQV
                     GNHGDGSPFDGAPTTGTNLLAHAFAPGAGLGGDVHFDDGDTWTTAQRTVLTSPFDLVT
                     VAAHEIGHALGLDHSTVNCSIMAAAYGASHRYLAPDDIAGIRALYGNRNPILISGRTC
                     SGATLSIPNLGTGPTITWSSNNTGIATVNAAGVVSRTGNGNGTVRITATIILPCGLNY
                     TEFVDIYIGVPPAPGFNTLTNQCIGGSDWELYLQAIPSVSMPPGTKYIWSRNGIDGSP
                     TTTDLYYTYEFPPSCMNIGIKVTNTCGTSAANVVTFCPPCSGFRMAVSPNPATTELNI
                     TIDDGESVIKSNKGANVLFTLYGMPAGNPVKTWTMATGQKTYKLSVSGIRKGIYIIKA
                     TIGSKTQTEKIIVE"
     gene            complement(5289..5663)
                     /locus_tag="PK768_04515"
     CDS             complement(5289..5663)
                     /locus_tag="PK768_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60264.1"
                     /translation="MKKLLTTMLLFVFIFSSAFTCNKENEQEFCNRNREVADTLVDRT
                     GTIYYNDPYKRWAINIDSSVASIDNSYIGIPCSFTSDFQKEGTKVKFSGVLKKLNEDE
                     IIQPKISTQELFYLEITSISKK"
BASE COUNT         1986 a         1161 c         1294 g         1908 t
ORIGIN      
        1 ttgccgacct ctcggagtga caattgtagg tagtatttaa atatataaat cttttatagc
       61 atatgtagta tattttaaat gaccatattt caattattta atcaaattta tacaaaataa
      121 gctaattgtg gtacaacata ataagctaat tttggtatgt cacaataagc taattttaga
      181 aactaaatgg aaggggcatg gcaacaccga acgacaaact cgctgaatca ctccaagttt
      241 tgcaggagct gcaaaacgga ggggttatcg ccattcgctc aaatcagcta tcgcggactc
      301 atcgtgaacg acttttaaaa caagggttct taaaagaggt tatgaaaggc tggtatatcc
      361 cagcaggtcc ggatgaactc cctggtgata gtacatcttg gtatgcttca ttctggcatt
      421 ttgcagcgga atatctgcac gaaagatttg gaaacaattg gagcataagc ccagagcaat
      481 ccgtcatgct tcatgcggca aattatgctg ttccaaccca attatatatt cgtgcttcgg
      541 gggcaagaaa tcatattacc gaacttatac acggcacttc tttattagaa atacgtgcgg
      601 cattaccgaa tgaagccgat cttatagaaa aagacgggct tagattattt tcattgagtt
      661 cggggctaat aaactgcaat gaaaattttt acacacgaaa ccccacggat gcacggacag
      721 cgttactgat gatacgagat tcatcggaag tattaggaat gttactcgat gggtcgcata
      781 gcgttgttgc aggaagattg gcaggagctt ttcgcaatgt cggtcgaaca agaatggcag
      841 atgaaatact tgatagcatg aaagcggcag gtttcaccgt tagagaaacc gatccgtttg
      901 aacataaaac accggttatc attccgcaac gagaacgatc tccatacatc aaaggaattc
      961 ggctgatgtg ggctaccatg agagaagtag tattgcagga atttccagtc gctcccggaa
     1021 tgccgccaga tattgaggcg tatataaaag aagtagatga taactacgta aacgacgcct
     1081 ataactccct ttcaatcgaa ggatacaggg ttacgccgga actcattgaa aaagtccgca
     1141 gcggcaaatg gcagcccgat ttaaatgagg atgatagaaa ccaatcgaac gcgctggctg
     1201 caagaggtta ttggcaagct tttcaggcag tcaaagaaag cgtaataaaa attttgaatg
     1261 gaaataacgc gggtaatgta accgataagg atcacggcac ttggttccgt gaattattag
     1321 gccccagcgt tacggcaggt attctgaaag catccgatct cgccggatac agaaacatgc
     1381 cggtctttat tagacgttcc catcacaccc ccccaaatgt cgatgcggtc agagatttaa
     1441 tgcctgtgtt atttgaaatg cttgcggaag aaaaagaagc cgccgttcgt gttgttctag
     1501 ggcattttat ttttgtatat atccacccat atcgtgacgg gaatggccgg attggaagat
     1561 ttttgatgaa cacgatgatg gcatcgggcg gctatccttg gacggtggtt ccgctagcaa
     1621 gacgagacga atatatggcc gcgttggaaa gcgcaagtgt tggccacgat attcggcctt
     1681 ttgctcagtt tttagcaagc cttcttccaa atgaatgatg tgatatgctt aaaaatttta
     1741 aacatcaata aaactttaca ccgctaaaaa tgaaacctat taactgacag gtgaagaata
     1801 agatttgccc ctgtgacatc tagaacatca ctttgattaa aacctttttg catagccaca
     1861 actaacgcgt cgtttgatgc ctggagagca tcgatctttt ggctgagatt tttaatcaaa
     1921 attgtaaaat cttccatcaa ttattaattg atgttgacac tgataggaca gtgtccaccc
     1981 gcgatttgag tgtaaagaaa tcaattaaac caatttaaaa aactaatctt ataatttatt
     2041 gattgtatgg aaattgatat catagatgat agtatccgta tttcagtgct aaaagcctga
     2101 aatctggaag ttgtgtaatg gaagggaaat aaaaaaaggg cttcatcggc tgaaaccctt
     2161 tctggtggct gctccccctg ctggacttga accagcgacc ctctgattaa cagttgtcca
     2221 aatctcaaaa aatttgtttg catatatttt cttttgtttg cataaaatac ttattaacat
     2281 tgtcttgcat gaattttgct gttatatagt gtcatttttt ttcctattaa ttttagtatt
     2341 gaggtcacat ttcaggtcac attcttaaga aagaatttag gagagaaaga gtctattagt
     2401 tacacgcaaa ttgaatgata tggaaagcga aaaagtaagc gttacttttt ttctggatgc
     2461 ctcaaggcca aattcagaag gcaaatgcct agtaaaactt aatatttatt gccgtccaga
     2521 taaacagcga tacccgacta tttttcacct tacgaaagaa gaatggaaga aaataaacgg
     2581 ccaaaaacta aaggacgagg aactaaaaat tgttcggaac aagctaaatg ctttaaagga
     2641 taaagtggaa agaattatta agggtttgac accattttct tttgtagcct ttgaagatgt
     2701 atatcttaga aataaaacaa acatagccgg cacgtcagtt aaaacatggt ttgaaaatta
     2761 tattgtcagt ttagaagtgc aagaaagaat aggatcagct atggcataca caacagcaat
     2821 gaattctctg atcggatata gaaagaatct tcatctgcaa gatataacac ctacattcct
     2881 tgtcacctat gaacactata tgattgaacg aaacgcttct cttagtactg ttggctctta
     2941 ccttaggcag ttaagggcaa ttattaatct ggccataagg aagaaagtcg tgtctgctga
     3001 taactatcct tttaaggaat atcagattcc ttctactcga aatgtgaaaa aagcacttcc
     3061 caatacggag ttaaaaaaac tgttggaata tgttcctgaa gatcaagaag aacgaaaggc
     3121 gcttgattat tggattctca tgtatttatt aagtggaatt aatcctgcag atgtcattcg
     3181 gctaaagccg gcaaacattg acaatcagta cctgaatttt tttcgcttca agaccaggta
     3241 tacaaagaaa aaagacttgc ggcctgtaaa ggtaggaata ccaaaaaggg caatggagat
     3301 aattgaaaga agaaaaaata tagaccccga taatgaatat ctatttccaa ttcttcatca
     3361 agagttaagt gcccggcagg caaagttcaa agggcaggat tttgcgaaat ggataaatga
     3421 taaaatggaa aaaataagga aggacttaaa aattagccaa aaagtacgaa ataacgaagc
     3481 tcgtcacact tacgctacgg ttatgaagcg taaaaatgtt gggatagttt ttatcaaaga
     3541 aacattaggg cacagctccc ttaacacaac tgaattgtat ttagatagtt tccctgatga
     3601 aactaaagtg gagtatgcaa atatgttaga gcagttatag ctagcacata ttttccagca
     3661 agcacttgga catactaata taaaaacaac ggagaattac ctggatagtt tcgagaaaga
     3721 atatgctgaa aaattaactg cattcaaaga gacgaattaa ttaattggct attaagagta
     3781 tcaataattt taatatacgc cgacagttcg ttatttggaa aatgggagcc atgtaaaaaa
     3841 tgtccggctc ccattttctt ttatgttatt cactaattta attattcaac gataattttt
     3901 tcagtttgag ttttgctgcc aattgttgct ttaataatat agatgccctt tctaattcct
     3961 gatacgctca gtttgtaggt tttttgtcct gttgccattg tccaagtttt tactggattt
     4021 cccgctggca ttccatacag cgtaaataga acattcgcac ctttgtttga ttttataaca
     4081 ctttctccat catctatagt aatgttcagc tcagtagttg ccggatttgg acttacggcc
     4141 atcctgaacc ctgaacacgg cggacaaaat gttacaacgt tagctgcact ggtgccacaa
     4201 gtgttggtga ccttgatgcc aatattcata caggaaggcg gaaattcata cgtgtaatac
     4261 aagtctgttg tggttggaga accatcaata ccgttacgac tccatatgta tttagttcct
     4321 ggaggcatag acacgcttgg aatagcctga agatataatt cccaatcaga tccgccaata
     4381 cattggttgg ttaatgtatt aaatcctgga gctggcggaa ctcctatata aatgtctacg
     4441 aattccgtgt agtttagtcc acaaggcaga atgattgttg ctgtaattct gactgtaccg
     4501 ttgccgttgc cagttctact gactactccc gcagcattaa ctgtcgcaat tcctgtgtta
     4561 ttgctactcc aagtaatagt tggtccggtt cctaagttag gtatagaaag tgtagcaccg
     4621 ctgcaggttc tgccagatat caatattggg tttctgttgc cataaagggc ccttatgcca
     4681 gcaatatcgt caggtgccaa atagcgatgt gaagctccat atgcagcagc cattatagaa
     4741 cagtttactg tggaatggtc tagtcccaaa gcatgaccaa tttcatgcgc cgcaactgtg
     4801 acaagatcga aaggggaggt taaaactgtt ctttgtgcgg ttgtccaagt atcaccgtcg
     4861 tcaaaatgaa catcgccgcc cagaccagcg ccaggtgcaa aagcatgtgc aagaagattc
     4921 gttccggtag taggggcacc atcaaagggg gacccatctc catgattgcc gacctgccat
     4981 gaaataacga tatctgccga ggccgcattt gaagcttcag tgaaggtaag tggcgtaaag
     5041 gcagaccaaa gtagcatgcc ttgaacaact gcagcccgtt catcgtttcc tgctatatcg
     5101 gcagttccat ttgagaagaa ataggtaata ttggttttat tccagtttcc aattatcaca
     5161 aaatttggaa taacaccaga tgacggcgga gttaattttc cttgatcaaa aagtacctgg
     5221 gcttggctgc ctatgatagt agttaacaat aaaatgataa ggtaaagttt tctcatacca
     5281 gtaaggtttt atttttttga aattgaagtg atttccaaat agaataattc ctgggtggaa
     5341 atcttcggct gaattatttc gtcttcattt agttttttca gcacgccgct aaattttact
     5401 tttgttccct ccttttggaa gtcgcttgta aatgaacatg ggattccaat gtaagaatta
     5461 tctatactgg caactgagga atctatatta atggcccatc tcttataagg atcattatag
     5521 tagattgttc ctgttctatc aactaaggta tcagcaactt ctctgttcct gttacagaat
     5581 tcctgttcat tttctttatt gcaagtgaat gctgaagaaa agatgaatac gaatagcagc
     5641 atggttgtta gcagtttttt catgtcatat tatttgctaa agtgacaaaa acagtctttt
     5701 cgacgttgca ttaaactgta taaattgttt taggatgcaa tgatcgtctc attaatttga
     5761 cataaatgta atactggcag ataagtttta gctcgttctg tgccttaaat ttttgtgtca
     5821 aaaaagcaat ttttattcac gtcatttcta atcatgatat ctaagaattc tttttgttaa
     5881 taaggtctgt tgtcaggatc gtttaggaca tttttctaaa acaaagcggg gctcaccctc
     5941 tattaccaca gtctcctgtt catgaaatct tttatcgaca attgacaaag cgcatggtag
     6001 acaagagaac ataaattaat atgagtattg ctatctgatt atttttcttg ctgtaaaatg
     6061 ttcgggtgcg tcaagaaagc aatttttttc ttacactttt atattcacca gcattgcgtt
     6121 gtttttgaat gtagttcttt aaaattctta ttgggtgagg gtttctggca attttggctc
     6181 tatgttaatt tcgtcatgga catcataaaa gcgagcactt ggtcgtccca aactttctga
     6241 tgtaagtagc tctgagccgt tgttttctat acccttgata atttatttat aagggctaat
     6301 aaaaaacagt tactaattta ataggcttaa ttggcacaag gtgtcattt
//
LOCUS       DAONUO010000091         6312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9255_length_6312_cov_3.132971, whole genome shotgun sequence.
ACCESSION   DAONUO010000091 DAONUO010000000
VERSION     DAONUO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9255_length_6312_cov_3.132971"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PK768_04520"
     CDS             <1..1300
                     /locus_tag="PK768_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60265.1"
                     /translation="WFTEENGSGTAYTRETNITEDVTLYAYWTYIGGSSGGGSTTTPS
                     TPAEQEEIETETETTFQISISGNVGTIIIDEDPWQTAAEEDITITVPSASGVSTYSID
                     MSMSILSKTDDLGSLTISTDIGSITVQSNMLTDVAGAEGDKAQITFGLGDKSSLPEDV
                     RDRIGDRPLIQLSLSIDGQQTSWNNPDAPVKVSIPYTPTEEELSNPESIVVWYIDGSG
                     NVVTVPNGHYDPATGTVTFYTTHFSDFAVAYNKVSFKDVPEDAWYNKAVSFIAARGIT
                     EGTGNDHFSPELNLKRGEFLVLMMRAYGIAPDEKPTGNFSDAGNTYYTGYLAAAKRLG
                     ISAGVGNNRFAPEKEITRQEMFTMLYNALKVIGQLPKGDSGRTMFDFSDASEIASWAK
                     EAMAHFVRTGTVSGSAGKLSPADTATRAEMAQLLHNLLAN"
     gene            1690..2440
                     /locus_tag="PK768_04525"
                     /pseudo
     CDS             1690..2440
                     /locus_tag="PK768_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            2590..3813
                     /gene="dpaL"
                     /locus_tag="PK768_04530"
     CDS             2590..3813
                     /gene="dpaL"
                     /locus_tag="PK768_04530"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HQA60266.1"
                     /translation="MSKNKELIKGSLIDKRKAKAKTSVDFISQEVVKRVNDFHKGFSE
                     YSVTPLHKLEALAKHVGVKNIFVKDESYRFGLNAFKVLGGAYAIGKYLADKLGMDISE
                     VSFEYLRSKEVREKIGEITFVTATDGNHGRGVAWAANQLGQGSVVYMPRGSSLTRLNN
                     IRKEGADASIIDGNYEDAVKLSEEMARKNDWVVVQDTAWEGYEEIPTRIMQGYGTIIY
                     EAMEQLKGHSINKPTHVFLQAGVGSFAGAILGYFVDKFGEERPITVIMEPDEANCHYL
                     SSLSGKREVVEGDMPTIMAGLACGAPNTISYDILMDYADGFLSCPDYVAARGMRVLAS
                     PLKGDPQVISGESGAAVGIGAVTLLMERDEYRDIREKLKLDESSVILIINTEGDTDPE
                     KYREIVWDGAYSSVE"
     gene            complement(3880..5802)
                     /locus_tag="PK768_04535"
     CDS             complement(3880..5802)
                     /locus_tag="PK768_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA60267.1"
                     /translation="MKTLFDRTKIGNMKLRNRIFMSPMGTGTDPDGGFSEQSRQYYED
                     RAKGGFGLIILGATTCTTKYEPKPCNVLDSQPMVERLYRVVESCHAYGAKVALQISAG
                     IGRMSFTDPNTPPYAASAVPCTYFPELLCRPLSIEQIKDIEDSFGNTAKWAKAAGCDA
                     IEIQGYGGYLIDQFMTELWNKRTDEYGGSLENRMRFPLNLIKQVREKCGEDFPILFKF
                     TLTHEIPGGRTIGEGLKIAKMLEEAGVDALHVDRGCFEVWYKPIPTVYDEDATKVDLA
                     AKVKEIVNIPVFCDGKLDDPIVAENAVREGKVDYISLGKQSIADPEWPNKVKSGRFDD
                     IRYCIYCNECLLGILEGRHTACSVNPRCGFENISQVTAAEEPKTVLIVGGGIGGMQTA
                     ITAADRGHKVTIWEKSDELGGLGIAAAAPDFKKSVKKYIQYLRNQVMKRNVNVVYNKE
                     ATVEDIVRFAPDKVVLATGAVPLLPPIEGLKDNPKVAMAVDCLVDKCETGDKVVVIGG
                     GLVGSETAIYLAEKGKDVTLVEMLHKLVPKEVINANNEQRLNVKIQEAGVKVMLNTKV
                     CCIDEEQVTVEKDGTKENIPYDTVIIATGMKANDQLEEELAKLIDEVYVIGDADSPRK
                     IWNAVHEGFHVAKNML"
     gene            complement(6080..>6312)
                     /locus_tag="PK768_04540"
     CDS             complement(6080..>6312)
                     /locus_tag="PK768_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60268.1"
                     /translation="ITMWDIYINLIKKAQEAGEILNHNPPEKIVEAITYLTVGISVIW
                     CNKNGSFDLISKNREMLEALLLVKDKPGSNSS"
BASE COUNT         1793 a         1322 c         1461 g         1736 t
ORIGIN      
        1 atggttcacc gaagaaaacg gttcgggaac agcgtataca agggaaacca acattactga
       61 agatgtgact ttgtatgcct attggacata tataggcggg agtagtggtg gcggcagtac
      121 aaccaccccg tcaacgcctg cagaacaaga agagatcgag actgaaacag aaaccacatt
      181 ccagatcagc attagcggca atgttggcac tataatcatt gacgaagatc cttggcaaac
      241 ggcagctgaa gaagacataa ctattacggt gccttcagcg tcaggcgtaa gtacctattc
      301 catcgatatg tcgatgtcta ttttgtcaaa gaccgatgat ctgggctcac tgactatcag
      361 tacagatatt ggcagcatca cagtacagtc taacatgctg acagatgtag ccggagcaga
      421 aggggataag gcgcaaatca ccttcggcct gggagacaaa tccagtctgc ctgaggatgt
      481 aagggatagg ataggtgaca gaccgctaat tcaactcagc ttatctattg acggtcagca
      541 gactagctgg aacaatcccg acgcgcccgt gaaagtgtcc atcccttata ctccgacaga
      601 ggaagaactg tcaaatcccg aaagcattgt cgtatggtac atcgacggtt ccggcaatgt
      661 tgtcacggta ccaaatggac attatgaccc ggcaactgga actgtaacat tctacaccac
      721 ccacttcagt gactttgctg ttgcgtataa caaagtgagc ttcaaagatg tgcccgaaga
      781 cgcatggtat aacaaggcag tcagtttcat cgcggcaagg ggcataacgg aaggcacggg
      841 caatgaccat ttcagccccg aattgaacct gaagcgaggt gaattcctcg ttctgatgat
      901 gagagcatat ggtatagcac cggatgagaa gcccacaggc aacttctctg atgccggcaa
      961 cacctactac accggatacc tcgccgcagc aaaacgcctc ggaatatccg caggagtggg
     1021 caacaatagg ttcgcccccg aaaaagaaat aaccaggcag gaaatgttca ccatgctcta
     1081 taacgcactt aaggtaatcg ggcaattgcc aaaaggcgat tccggcagga ctatgttcga
     1141 cttctctgat gcttcggaga tagcctcttg ggcaaaggaa gcaatggccc atttcgtaag
     1201 aaccggcacg gtcagtggct ctgctggcaa actctctcct gcagatacag ccacccgtgc
     1261 agaaatggcc cagcttctgc ataacctact ggccaattga atatttcggg acaggtacaa
     1321 aatattcaca tcatgagaac aggcaaatct acttttgacc gaggctattg aaacaaggcc
     1381 gccagttgag aagataatct tctcgcctgg cggccttgac tattaactct cacatgatgc
     1441 tatactttaa attataattg agctcaaaaa tacacacgtg tgtggaggat ataccatgtc
     1501 agtcctgccc aaacgcaaaa cccagatgaa cttgtaaccg agatttgttt cccagtaaaa
     1561 gagaaatgaa taatgagttg aacttaagca gttaaatatt gatagagata tgctataata
     1621 aaatctaagc tattgtagaa gaccaattat gaatctgctg cttaaggatc aattctggag
     1681 gtgtaaaata tgaaaattgc gtcgtttcag ctaaacggca atgtgtcata tggcgtcatt
     1741 gcaaataaag agatcatatt aatagaagga gatatctttg gtgattttgt tgtaacagat
     1801 actcggattt ccttggacga aataaaaata ttgccacctg tgatacccag caaagcagtt
     1861 tgcgtgggtc taaactatca tgaccacata gaagaaacta attccaaggt tccggaaagt
     1921 cccgtgcttt ttatcaagcc ttctaccgca gttatcggtc acatggatga tataaagtac
     1981 cctgaattgt ccgagagtag attatgaagg ggaacttgcc gtagtaatcg gcaaaacagc
     2041 aaggaacata agtgcgaatg aagccgagga atatattctt ggatacacat gtagcaatga
     2101 cgtaactgcg agagatcttc aaccggctga tggccagtgg acgatagcaa aatcatttga
     2161 tactttttta cctctcgggc catgtataga gacggaagta gatccacgaa atcttgaaat
     2221 acgaacttac ttaaatgatg agttaaagca gtcatccaat acagggcata tgattttcga
     2281 cccatatact ttggttagct atgtttcaaa gataatgacc cttcttccgg gagatgtaat
     2341 actcaccggc acgccctccg gaattggccc catgaaaaaa ggtgataaag ttgtcgttga
     2401 aatagaaagc attggctgtt tagctaacta tgtgaaatag tgaatttttg gggacaggtg
     2461 aatttttggg gacaggtaca aaatattcac ttgtgaatat tttgtacctg tccccaaaaa
     2521 ttcacctgtc cccaaaaatt catcaaatcg cctcaaatac tcatgaaaaa tcggtaaggg
     2581 gaggaagaga tgtcaaagaa caaagagcta attaaaggaa gtctaattga caaaaggaag
     2641 gctaaagcaa aaacaagtgt ggattttatt tctcaagaag tggttaaaag ggtaaacgat
     2701 tttcacaaag gctttagtga gtattcggtg acaccgctgc acaaattaga agctttggca
     2761 aaacacgtcg gtgtaaagaa tatcttcgta aaagatgagt cctataggtt tggattaaat
     2821 gcttttaagg tcttaggtgg tgcttatgca ataggcaagt atctagctga taaattaggc
     2881 atggatatat cggaagtgtc ttttgaatat ttgcgttcta aagaagtgag ggagaagata
     2941 ggagaaatca cctttgtgac cgctacggat ggcaatcacg gaaggggagt tgcatgggct
     3001 gccaatcaat tggggcaagg gtcggttgtc tacatgccga ggggctcatc ccttactaga
     3061 ttaaacaaca taagaaaaga aggggcagat gcgtctataa ttgatggaaa ttatgaagat
     3121 gcagtaaagc tttctgaaga aatggccagg aagaacgact gggtagttgt ccaagatacg
     3181 gcatgggaag gatatgaaga aatacctacc cggattatgc agggatacgg caccataatc
     3241 tatgaagcta tggaacaact aaaggggcac agcattaata aacccactca tgtattcctt
     3301 caagctggag tgggatcctt tgctggagcg attcttggct attttgtaga taaatttgga
     3361 gaggaaaggc caataacggt tatcatggaa ccggatgaag cgaactgtca ctatttatct
     3421 tccttaagtg gcaaaaggga agtagttgaa ggagatatgc ctaccattat ggcgggtctg
     3481 gcatgtggtg ccccaaacac cataagctac gatatattga tggattatgc cgatggcttt
     3541 ttatcgtgtc ccgactatgt tgcggcaagg ggcatgcgag tacttgcttc acctctaaaa
     3601 ggagatcccc aggtaatatc gggagaatca ggtgctgccg tgggaattgg cgcagttaca
     3661 ttgttaatgg agagagatga atacagggat attagggaga aactaaaact agatgaaagt
     3721 tcagtaatac tgataataaa cacagaaggg gatactgatc ctgaaaaata cagggaaata
     3781 gtctgggatg gggcatacag ctctgttgaa tgaataacca taaactatgg acctgcctgg
     3841 aggtctttcg tttggggccc cctggcaggt cctcatcaat tacagcatat ttttcgctac
     3901 atgaaatcct tcgtggactg cgttccaaat cttcctggga gagtctgcat cgcctattac
     3961 gtaaacttca tcgataagct ttgcaagttc ttcttccagc tggtcgttgg ctttcattcc
     4021 agttgctata ataactgtgt cgtaaggaat gttttctttg gtgccgtctt tttctaccgt
     4081 tacctgttct tcatcgatgc agcatacttt ggtatttagc attactttga caccagcttc
     4141 ttggattttt acgttaagtc tttgttcatt gttggcattt atcacttctt ttggcacgag
     4201 cttgtgcagc atttcaacca gtgtcacatc tttgcccttt tcagcaagat aaatagcagt
     4261 ttcgcttccc acaagccctc ctccaatgac cacaactttg tcgccggttt cgcatttatc
     4321 aactaggcaa tccactgcca tggccacctt aggattgtcc tttaagcctt caatgggtgg
     4381 aagcagcggg actgcaccgg tcgccaagac aaccttgtca ggtgcgaatc ttactatatc
     4441 ctctacagta gcttctttat tgtataccac gtttacattg cgtttcatta cttggtttct
     4501 caagtactgg atgtatttct ttacggattt cttaaaatca ggagctgctg ctgcaatgcc
     4561 cagtccgccc agttcatcac ttttttccca tatggttact ttatgtccgc ggtcggctgc
     4621 agttatggca gtttgcattc cgccaatgcc accgcctact attagcacgg tttttggttc
     4681 ctcggcagct gttacttggc tgatgttttc aaaaccacat cttgggttga cggaacaggc
     4741 agtgtggcgg ccttcaagga tacccagtaa gcactcattg cagtagatgc agtatcttat
     4801 gtcgtcaaat ctgccgcttt tcaccttgtt gggccattca ggatcggcaa ttgactgctt
     4861 gcccagggag atatagtcta ccttgccttc ccttactgca ttctcagcta ctatagggtc
     4921 gtctaattta ccatcgcaaa ataccgggat gtttactatt tctttaactt tggctgcaag
     4981 gtctactttt gtagcatctt cgtcataaac agttggaatg ggcttgtacc agacttcgaa
     5041 acatcctcta tcaacatgta gtgcgtcaac accggcttct tcaagcattt tggcgatctt
     5101 taagccctct ccgattgtcc taccgcccgg aatttcgtgg gtgagggtga atttgaagag
     5161 aattggaaag tcttcgccgc atttttccct gacttgctta atcaggttca aggggaacct
     5221 catcctgttt tccaagctgc cgccgtattc atcagtgcgt ttgttccaaa gctctgtcat
     5281 gaactggtca attaggtaac cgccgtagcc ttgaatttct attgcgtcac atcctgcggc
     5341 ttttgcccat ttggctgtgt ttccaaagct atcttctatg tccttaatct gttcgatgga
     5401 aaggggtctg catagtaact cggggaaata ggtgcaggga actgcacttg cggcataggg
     5461 gggagtgttg gggtcggtga aggacatgcg gccaatacct gcacttatct gtaaagctac
     5521 tttagcgcca taagcatggc aggattccac aaccctgtac agccgctcaa ccattggctg
     5581 actgtctagc acgttacagg gcttgggctc atacttggta gtgcaggtgg tggcacctag
     5641 gatgattaga ccaaatccgc cttttgccct gtcctcatag tactggcgag actgttcgga
     5701 gaagcctccg tcagggtctg taccggtacc catgggggac atgaagattc tgtttctcag
     5761 cttcatgttg ccaattttag tgcggtcaaa aagtgttttc atgaaaagac ctccctttag
     5821 gtttttattt tagggtgaac ttcagtttag ttaaattgat cacttcccgg gtagtgacca
     5881 tttgacatct aggtttaaag atagaattca cattcacata tgaaagaaaa aagaaaaaca
     5941 acatgtgtat aaaaaagtcg tacaagtgtc cgactatgtc tgacaaaatt ataacattaa
     6001 aaagattttt tggcaataga gatttttaag tttttacaat atcgttttaa aaaataaaaa
     6061 agccgtgctt ctgcacggtt taggaagaat tgctaccagg cttgtcttta acgaggagaa
     6121 gagcttccag catttctctg tttttggata tcaaatcgaa gctgccgttt ttgttgcacc
     6181 aaatgacaga aattcctacg gtgagatagg ttatggcttc tacgattttc tccggaggat
     6241 tgtggtttag gatttctccg gcctcctggg ctttctttat gagatttatg taaatatccc
     6301 acatggttat ct
//
LOCUS       DAONUO010000092         6306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9264_length_6306_cov_3.473044, whole genome shotgun sequence.
ACCESSION   DAONUO010000092 DAONUO010000000
VERSION     DAONUO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9264_length_6306_cov_3.473044"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK768_04545"
     CDS             complement(<1..354)
                     /locus_tag="PK768_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60269.1"
                     /translation="MDKEQLKEQVSQAIDKHRDEIIRIGEQIFNNPELGYKEYKTSAL
                     VKEVFRNLDLPYEENLAITGVKALAEGKKHDVKVAIMGELDAVVSHTHPCADPGTGAA
                     HACGHNAQIASMLGAA"
     gene            complement(459..1667)
                     /gene="ilvA"
                     /locus_tag="PK768_04550"
     CDS             complement(459..1667)
                     /gene="ilvA"
                     /locus_tag="PK768_04550"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HQA60270.1"
                     /translation="MDVTFRDIQEARSHLKDVVYKTGLVHNTTFSEMTGNFIYLKTEN
                     LQKTGSFKLRGAFNKIANLPKEQKQYGVIAASAGNHAQGVAMAATAYGIKSTIVMPKH
                     APLSKIAATKSYGAEVVLYGEVYDEAYEKARQIQKQSGAVFVHPFDDREIIAGQGTIG
                     IEILEDVPDADIIVVPIGGGGLISGIAIAAKHIKPEVKIIGVQTKNLPSMARSLSQNH
                     ITTVEGPATIADGIAVKTPGELTFKIVKKFVDDIVTVDEDEIANAILLLLERAKVVSE
                     GAGAASVAAILNRLDIVRNKKIVALISGGNIDVNMLSRIIDKGLVKSGRKIFVNTLIP
                     DRPGTLSQLLNLIAETGANVLSITHDRATADIPLGYAKVKLELETADVQHVVKIKELL
                     VQNNYSFSIQ"
     gene            1905..3524
                     /locus_tag="PK768_04555"
     CDS             1905..3524
                     /locus_tag="PK768_04555"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HQA60271.1"
                     /translation="MSNVYIFGHKNPDTDSIAAAIAYAEFKSQLSEGNYIPARLGEIN
                     SETRFVLDYFKIPVPPLLENVYAQLSDIAFDRPVCFTKDAPISEVWNAMMKQGIKTAI
                     VVDDGGRFLGIASLGDIARANLEISNDFGKFIVPLENIINTLDGKALHLASEFFSGKL
                     AVAAMNIEDVKRRLSKETLLIVGNREDVLFAAVEKKINTIIVTGNGYVSDDILNLARE
                     KGVNLIKVPHDTFNTVKLINHSIPIHYVMKNKGLITFGIREYMDDVKDTMLRYKYRYF
                     PVLEDKKPVGMLTRRHILDFVGKKVILVDHNEKTQSVEGLEQAQVLEVIDHHRIGNME
                     TAMPIIFINKPVGSTCTIIYSLHKENSISPSPPIAGIMCAAILSDTLIFKSPTCTPID
                     MRAAEELAEIAGIDLNEFARAMFEAGTSLKGKTEEEIFFTDFKEFNIGGYQIGVSQVN
                     IYNTDLERLKHKLLGFMERLKEDKGYDMLLMMLTDIINEGSEFLHVGGHRELLSRAFD
                     LEIKGESFYLPNVVSRKQQVIPRLITAISAQ"
     gene            complement(3591..4250)
                     /locus_tag="PK768_04560"
     CDS             complement(3591..4250)
                     /locus_tag="PK768_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60272.1"
                     /translation="MYQSPIVIERALTENGELQLQRRGEDYEIIFNGTFLMATYNGES
                     EKLLVRWAIEKAVSPKWILIAGLGVGFSLAEAQKHKYIEKITVVEIEQKIIEWHRKYL
                     SNFSEGALSQSHVEVVQGDFIEWMKTSGEKYDVVCLDIDNGPDWVVLENNKKLYSDNG
                     LCSLLKVMNKGSAASFWSASRSRCFETLLKRYFEGVCIKEVPHQKGEPDYIYLAYNPK
                     L"
     gene            complement(4283..4789)
                     /locus_tag="PK768_04565"
     CDS             complement(4283..4789)
                     /locus_tag="PK768_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60273.1"
                     /translation="MKRVPLILICLLLIAILAAGCFSKTADQSPAELDESTGASPKES
                     GENPPQSGTDENHEPISDAPETPVASGVSFIIDEPGEGQLVKSGSELTIRGKSRIREF
                     DIEVEDGHEILGMAHVSVSGQSQELEEFEATIELKEHTSPSGMIIFVTEDEAGERQEE
                     LILPVKFE"
     gene            complement(4844..5608)
                     /locus_tag="PK768_04570"
     CDS             complement(4844..5608)
                     /locus_tag="PK768_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HQA60274.1"
                     /translation="MADYNEKQLEARIRDIFTIARKNREKKASNFLDPGQQQIALDVA
                     RSFPGMDFYFDGGHEEAERKILVAYPEDLRDEPFMLPLGALRVTPRDPDEYPGHRDYL
                     GAILGLGINREKIGDILVGKRNADVIVKQEIMEYIGLNLIKVGNVPVDVDEISLKELL
                     QPERPYKELKGTVASLRLDAVAGIAFGISRTKMAPFIKGENLRLNFRVVKDPATPVKE
                     GDVISANRLGRAKIVEVGGQSKKGRTYVKVHRFISR"
     gene            5839..>6306
                     /locus_tag="PK768_04575"
     CDS             5839..>6306
                     /locus_tag="PK768_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase"
                     /protein_id="HQA60275.1"
                     /translation="MAKLFTPFKIKDLTIKNRIMMPPMCMYSSDDTGRAMPWHYVHYV
                     TRAVGQVGLIIVEATAVEKRGRISESDLGLWDDNLVDGLTRIVEEVKKQGARIGIQLN
                     HAGRKCTAPHENIIAPSSIPFDESYKVPAEMTKDDIKQVVEAFAEAAKRANMAG"
BASE COUNT         1643 a         1541 c         1324 g         1798 t
ORIGIN      
        1 ggcagcccca agcattgacg ctatctgagc attgtggccg caggcgtggg cggctccggt
       61 accggggtcg gcacagggat gcgtgtggct tactaccgca tccagttcac ccataatagc
      121 gaccttaaca tcgtgcttct tgccttcggc taaggccttg actcccgtta tggctagatt
      181 ttcttcataa ggcaaatcta agttccgaaa aacttctttc accaaagcgg aagtcttgta
      241 ttctttgtag ccgagctcgg gattattaaa aatctgttca cctatccgta tgatctcatc
      301 ccgatgctta tcaatagctt gtgatacctg ctcttttaac tgttccttgt ccaccttatc
      361 gcctccaaat aaattgtaca aatcgtgtcc accaggcagc gacatatttc tttctattat
      421 actacgcttt tcacaataaa tgaagtcttt tttaatgttt actgtatgga aaaactgtag
      481 ttattttgga ccaaaagttc cttaattttg accacatgct gcacatctgc ggtttccagc
      541 tcaagcttga cctttgcata tcccaaggga atgtccgcag tggctcggtc atgggttatc
      601 gacaggacgt ttgcgccggt ttcggctatc aggttgagca actggctaag ggttcccggc
      661 ctgtccggta tgagggtatt aacaaaaatc ttccggccgc tctttaccag gcccttgtct
      721 attatcctgg acagcatgtt cacatctata ttgccgccac ttatgagggc aactattttt
      781 ttattcctga caatgtccag gcggttcagg atagcggcta cggaagctgc cccggcacct
      841 tccgagacga cctttgccct ttctaataat agaagtatgg catttgcaat ttcatcttca
      901 tcaacggtaa ctatatcatc tacgaacttt ttaactattt taaaagtaag ctctcccgga
      961 gtcttaactg caatgccgtc agcaatcgtt gcgggacctt cgacggttgt tatgtgattt
     1021 tgcgaaaggg accttgccat tgaaggcaag tttttcgttt ggaccccgat tatcttgact
     1081 tccggcttta tgtgttttgc agctattgct atgcccgata tcaagccccc tccgccgatt
     1141 ggaactacaa ttatgtccgc gtcgggtaca tcctcaagta tttcaatccc gatggtacct
     1201 tggccggcta taatctccct gtcgtcaaag gggtggacaa aaactgcacc gctttgtttt
     1261 tgtatttgcc ttgccttctc gtaggcctca tcatatactt caccgtagag gaccacctcc
     1321 gcaccgtagc tcttagttgc agcaattttc gataaaggag catgcttggg catcactatc
     1381 gtcgatttga tgccgtatgc cgttgcagcc atggctaccc cctgggcgtg attccccgcc
     1441 gatgcagcta tgacgccgta ttgcttttgc tcttttggca ggttggcaat cttattaaag
     1501 gcacctctca gcttaaaaga cccggttttt tgcagatttt cagtctttaa gtagataaag
     1561 ttccctgtca tctcgctaaa agttgtgttg tggaccaggc cggtcttgta cactacgtct
     1621 tttaagtggc ttctagcttc ctgaatatcc ctaaaagtga catccatgtt ttcaccttct
     1681 aatttttatg ttgttaagga agctgcctgc atgttaaact caggctgtcc catctcctat
     1741 ttccccggtt agagtattcc ataaaaaagt aaaaaatcct tcttgaattt ataatatttg
     1801 gattggacat ttttgaaaag cggtgctatc atattgaaaa agtctcccac tttatgttca
     1861 cttatattgt tgatatatct tattctagtg aaaggacctt cagtatgagc aatgtgtata
     1921 ttttcggtca caaaaatcct gataccgatt ccatcgcggc agccatagcc tatgcggaat
     1981 ttaaaagtca gctttcagag ggaaactata tccctgccag gctcggcgaa ataaacagcg
     2041 aaacaaggtt cgtcctggat tacttcaaaa ttcccgtgcc ccccttgttg gaaaatgtgt
     2101 atgcccaatt gtccgacatc gcttttgaca ggccggtatg ttttacgaaa gatgccccga
     2161 tttccgaggt ctggaatgcc atgatgaagc agggcataaa gacggccatt gtagttgacg
     2221 atgggggtcg atttttggga atagcgtccc taggtgacat agccagggcc aaccttgaaa
     2281 tctccaatga ctttggcaag tttattgttc ccctggaaaa cattatcaat accttagacg
     2341 gcaaggcctt gcacctggca tcagagtttt tctccggaaa actggcagta gccgccatga
     2401 acatagaaga tgtaaaaagg cgcctatcaa aggaaaccct gctcatcgtg ggaaatcgtg
     2461 aagatgtgct gtttgccgcc gtggaaaaaa agataaacac gattatcgta actggcaacg
     2521 gctacgtttc agatgatatc ttaaacttag caagggaaaa aggcgttaac ctgataaaag
     2581 ttccccatga caccttcaat accgtaaagc tcattaatca cagcatcccc attcactatg
     2641 tcatgaaaaa taagggctta atcaccttcg gaatccggga gtacatggat gacgtgaagg
     2701 ataccatgct caggtacaag tacaggtact ttcccgtatt ggaagataaa aaacccgtag
     2761 gtatgcttac caggaggcat atcttggact ttgtagggaa aaaagtcatt ttggtggacc
     2821 acaatgaaaa gacccaatcg gtagaaggcc tggagcaggc ccaggttttg gaagttatcg
     2881 accaccacag gataggcaac atggaaactg ccatgcccat aatattcata aataagcctg
     2941 tcggctcaac ctgcacaata atctatagcc tgcacaagga aaactccata agcccatccc
     3001 cacccattgc cggtataatg tgtgctgcca ttttgtctga taccctgatt tttaaatcgc
     3061 ctacctgcac tcctattgac atgagggcgg ccgaagaact ggccgaaata gccggcatcg
     3121 atttgaacga gtttgccagg gccatgtttg aggccggcac gtccttgaaa ggcaaaacgg
     3181 aagaagagat tttctttacg gactttaagg aattcaacat agggggttat caaatcggcg
     3241 tcagccaggt caacatatac aacaccgacc tggaaaggct aaagcacaag cttctcggct
     3301 tcatggaaag gctaaaggaa gacaagggct acgacatgct gctgatgatg ctgaccgaca
     3361 taatcaatga aggctctgaa tttctccatg taggcggcca ccgggaactg ctctcccggg
     3421 cctttgacct ggaaattaaa ggggaaagct tctatctgcc caatgtggtg tcccggaagc
     3481 aacaggttat cccgagacta ataacggcaa tatccgccca gtagtcggca atccggcaaa
     3541 gtaaaaaatg attaaaaaaa tacgcaggct caaaacctgc gtatctttta ttataatttc
     3601 ggattatagg ccaagtatat gtaatcgggc tctccctttt gatggggcac ttctttgata
     3661 catacgcctt caaagtatct tttaagcagt gtttcaaaac accgggacct tgaagcgctc
     3721 caaaaggaag ccgcgcttcc cttattcatg accttgagta agctgcaaag cccattgtca
     3781 ctgtaaagct tcttattatt ttccaaaaca acccaatcgg gaccgttgtc aatatcaaga
     3841 cataccacgt cgtatttttc cccggatgtc ttcatccatt ctataaagtc tccctgtacc
     3901 acttccacat gagattggct cagggcgcct tctgaaaagt tggagaggta cttccggtgc
     3961 cactctatta tcttttgttc aatttccaca accgtaattt tctcgatata cttatgcttt
     4021 tgtgcctcgg ccagggaaaa tcctacaccc aatcccgcaa tcaatatcca ttttggggaa
     4081 accgcttttt caatggccca cctcactaat agcttctccg attctccgtt gtaagttgcc
     4141 atgagaaagg taccgttaaa gattatctcg tagtcttctc cccttctctg aagctgcagt
     4201 tccccatttt ccgtaagtgc ccgctctatt actatcggac tttggtacat gttttcatcc
     4261 ctttaatcca catcttgtga catcactcaa attttacggg taaaatcagt tcctcctgcc
     4321 tttcgccggc ttcatcctca gttacaaata taatcatacc cgaaggactc gtgtgctctt
     4381 taagctcgat ggtagcctcg aattcctcca gttcctggga ttggccggat acgctcacat
     4441 gagccatgcc caggatttcg tgaccgtcct ctacctctat atcaaactct cttatacggc
     4501 ttttccccct gatggtaagc tcactgcccg attttaccaa ttggccttcc ccgggttcat
     4561 cgattataaa gctgacccca gaggcgacag gtgtctcagg agcatcggaa atgggctcat
     4621 ggttttcatc tgtgccgctc tgtggagggt tttccccgga ctctttggga cttgctcctg
     4681 tgctttcgtc caactccgcc ggactttgat cggcagtctt tgaaaagcat cctgctgcca
     4741 atattgctat taatagtagg caaatcaaaa ttaaaggtac tcttttcact ttatcacctc
     4801 ctacatatat ggacgcagaa aatcccgaga ggttcctcaa aagctacctc gatatgaacc
     4861 tgtggacttt tacataagtg cggccctttt tgctctgacc gcccacctct actatctttg
     4921 ccctgcctag gcgatttgcc gaaatcacat ctccctcttt aacaggagtt gcagggtctt
     4981 tcactactct gaaattcaac ctgaggtttt cgccctttat aaaaggggcc atctttgtcc
     5041 gggaaatgcc aaaagctata cctgccacag catcgagcct taaggaagcc accgtgccct
     5101 ttaattcttt atagggcctt tcgggctgaa gaagttcctt taaggatatc tcatctacat
     5161 caaccggtac atttcccact ttaataagat ttaaccctat gtattccatg atttcctgct
     5221 taactataac atcggcattc ctttttccaa ccagtatgtc tcctattttc tccctgttga
     5281 tgccaaggcc aagaatagcc cccaggtaat ctcgatggcc gggatattca tccggatccc
     5341 tgggagttac cctcaacgcg cctaagggca gcatgaaggg ctcatccctt aaatcctccg
     5401 gatatgccac cagaatcttc cgctcggctt cctcatggcc accgtcaaaa taaaaatcca
     5461 tacccggaaa gctcctggcc acatccaggg ctatctgctg ctggccggga tctaaaaagt
     5521 tggatgcctt tttttcacgg ttttttcttg ctatagtgaa tatatccctt atccgggctt
     5581 ctagctgttt ttcattataa tctgccaata gattcaccac caattaagct ttatttaaac
     5641 gtctagtaga ggattgaaac tggcaaattc agttcctgct ttaaatgaac cgattattac
     5701 atatcactta ttatatgata acatatagca ttcctaaatc aaacaaattg taatggttag
     5761 tcccaacagg tactgtttaa aaatttgccg gtatagtaaa ataatagtaa aactttgtat
     5821 tatcggaggt gacttcgtat ggcgaaactt tttacccctt ttaagattaa ggatttgaca
     5881 ataaaaaata ggatcatgat gcctcccatg tgcatgtaca gcagtgacga cacgggtcgc
     5941 gccatgcctt ggcattatgt tcactacgtt accagggctg tcgggcaggt gggtctaata
     6001 atagttgagg ccaccgctgt agagaagagg ggcagaattt cggaaagcga cctgggactt
     6061 tgggacgata atctagtgga cggcttaact cgtatagtag aagaagtgaa aaaacagggt
     6121 gctcgtatag gtattcagtt gaaccatgcc ggcagaaagt gcacggcgcc gcatgaaaac
     6181 attatagccc ccagttcgat cccctttgat gaaagctata aagttcccgc tgaaatgact
     6241 aaagatgaca taaaacaggt ggtggaagct ttcgcagaag cagccaaaag ggccaatatg
     6301 gccggc
//
LOCUS       DAONUO010000093         6265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9340_length_6265_cov_4.445572, whole genome shotgun sequence.
ACCESSION   DAONUO010000093 DAONUO010000000
VERSION     DAONUO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9340_length_6265_cov_4.445572"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK768_04580"
     CDS             complement(<1..608)
                     /locus_tag="PK768_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60276.1"
                     /translation="MIHKSMRLLLVGVFLMLFVAGVAFASAKFSDVGDYWATEAIYWA
                     AEKGIVEGYPDGTFKPEKYVTEAEFAAILARYVTNTDKQKIAERRPGEHWAQSIYDEL
                     RQWELPLHGYDSDFGKDAAIFRGDVARVIAAKNGFNLDERQAVYYMYENDLSAGMVPG
                     RMTFESYRVDLPLQRDQITQFIKLLNNKGHTTFLGKPSPKGNA"
     gene            complement(805..1140)
                     /locus_tag="PK768_04585"
     CDS             complement(805..1140)
                     /locus_tag="PK768_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60277.1"
                     /translation="MNLSITFSKYGKREFEASKLTIDELKKSVRKAVQEKNKTGYVFF
                     EATSDNNTKYLIDGTLYNNGFLLQGVKRLYPHKIEENGKVEVVWRPKLEGPTAREYFT
                     IARRESPPL"
     gene            1645..3210
                     /locus_tag="PK768_04590"
     CDS             1645..3210
                     /locus_tag="PK768_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQA60278.1"
                     /translation="MSENNKFKESLLDKNTMSITWELVPGRGAHEKEQRDVILNAEKA
                     SKGGKIHALTLTDNPGGNPAISPEILAAEIVQMGIEPLVHFTCKDKNSNQIESLLYAL
                     DRVGVRNLLVMTGDYMYSGFFAKAKPVFDLEPMHVLTMISAMNEGLEYKVHKGTKKHK
                     PSDFYAGVVCSPFKKTEAELMCQYYKLKMKVASGAKFIVSQIGFDARKIHELIQFVRL
                     NNWDIPVIGNIYVLSYGTGRLMNQNKIPGCVVTNKLLAELEEESKAPDKGKGARLLRA
                     AKMYTIMKGMGYDGVHIGGHGTKYEDIEYIIDKGEELAKDWISLIPEFDYSFPDGFYY
                     FEKDEKSGLNTDKIANRKGRTLDAKVGFVYHMSNLIHALMLEPGTPLWKPMRAIANMI
                     DDTALERPYHFIERMTKTMLYDCKDCGDCALPDIAYLCPMSQCPKNIRNGACGGSYDG
                     WCEVYPGKKKCIFVRAYARLKSSGKEDHLDHEVIPPWNVDLNQTSAWINLYLGRDHNA
                     KKYGVKKVDSKKQ"
     gene            complement(3396..4169)
                     /locus_tag="PK768_04595"
     CDS             complement(3396..4169)
                     /locus_tag="PK768_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HQA60279.1"
                     /translation="MDYKGFTEEIKKMCNIDLSNYKEKQMKRRIESLIRRNGHESYDS
                     YLELLKRSKHHLKEFLDYITINVSEFFRNPAQWQVLEREIIPDLLKYKKRLRVWSSAC
                     STGEEPYSLAMIFDKMMIKSRVEIIASDIDRVAIEKAQQGVYTAKGITNIPSAMVKTY
                     FEQEDDKYIIKDHIKNMVNFKILNLLEDNYPTGCDLILCRNVMIYFTEDTKEKLYKKF
                     YDALSDRGVFFVGSTEQIIMPYKYGFSNLKNFFYQKILE"
     gene            complement(4421..4795)
                     /gene="speD"
                     /locus_tag="PK768_04600"
     CDS             complement(4421..4795)
                     /gene="speD"
                     /locus_tag="PK768_04600"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HQA60280.1"
                     /translation="MKNLGRHILAEVYDCDPNILNDRELIEEILVKAALEAGAEVREV
                     AFHKFSPQGVSGVVVISESHLAIHTWPELGYAAVDVFTCGDRVNPWDACNYISDGLGS
                     KHMDATEVKRGLFDEPVKVINL"
     gene            complement(4920..5963)
                     /gene="fni"
                     /locus_tag="PK768_04605"
     CDS             complement(4920..5963)
                     /gene="fni"
                     /locus_tag="PK768_04605"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HQA60281.1"
                     /translation="MKESRKLRKKEHIKYSLLLEERLKRNEFEDITILHNCLSEVNLK
                     EIDISTNLQDIKLASPIIINAMTGGMEEGEKINSQLSRIAKKLNIAMAVGSQKIALDN
                     PKSARSFEIVRKLNPDGIIFANLGADATVEEANMAIEMIKADALQLHLNVPQEVIMKE
                     GRRDFRGTVDNIAEIVRNVRVPVIVKEVGFGIAREEAVELLEKGVKIIDVGGTGGSNF
                     IAIERLRRGSNPLKKFEDWGIPTPISLIEVLTTVGSKADVIASGGLKDGLDASKSIAL
                     GAKAVAYAGSILHILYNKGPSGLEKHILQIEKEIKYVMAMTGASNLAELRKRPVIIKG
                     KTYHWMKYRGISI"
     gene            complement(6025..>6265)
                     /locus_tag="PK768_04610"
     CDS             complement(6025..>6265)
                     /locus_tag="PK768_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQA60282.1"
                     /translation="GLVHISQISKDHVSNPEDVLKTGENVKVKIMDINAKEKRMSLSI
                     REAQDENDNRQVYEKTNENNGVTIGEMVGDLFEKK"
BASE COUNT         1792 a         1321 c         1032 g         2120 t
ORIGIN      
        1 gcattgcctt ttggtgaagg cttgcctaaa aaggtggtat ggcctttgtt gttcaaaagc
       61 tttataaatt gggttatctg gtctctttgt aaaggcaaat ctaccctgta actttcaaac
      121 gtcatccgtc ccggtaccat acctgccgac aggtcgtttt catacatgta atacactgcc
      181 tgtctttcat cgaggttaaa accgttcttt gcggcaatta cccttgctac atcgccccgg
      241 aatatagcag catctttgcc aaaatcacta tcgtatccat gcaaaggcag ctcccactga
      301 cgaagctcat cgtagattga ctgtgcccag tgttcacctg gacgcctttc agcgattttt
      361 tgtttgtccg tattcgtgac ataccttgcc aatattgccg caaattctgc ctccgtgaca
      421 tacttttcag gcttaaacgt tccatcagga tagccttcca caatcccctt ctccgcagcc
      481 cagtaaattg cctctgtcgc ccagtagtct cccacgtcac taaattttgc cgatgcaaaa
      541 gctactccag ctacaaataa catcaaaaaa acaccaacca ataataaacg cattgattta
      601 tggatcaaaa tatcgtcctc cttactcctt atatacagtg acctccccca cctctaaaga
      661 agatgggctt cctctttcat agagagaaac ttatacgtac atagggattg ctccccagta
      721 tccgtacagc ggtttctctc tccggaggct ttacttcgga ccagtccagc cctacttgtt
      781 tagctctagc ctctcatccc acccttaaag gggtgggctt tcccgacgag cgatcgtaaa
      841 gtattctctc gctgttgggc cctctaactt tggcctccaa acaacttcta cttttccatt
      901 ttcctcaatc ttatgagggt atagcctttt taccccttgc agcaaaaatc cgttattata
      961 taatgttccg tctattaaat atttagtatt gttatcactc gttgcttcga aaaacacata
     1021 gcctgttttg tttttttctt gtactgcttt tcgaactgat ttttttaatt catctattgt
     1081 taatttactt gcctcaaatt ctcgcttgcc gtattttgaa aatgttatac tcaaattcaa
     1141 ttaattaact cccttctttt gttattttcc aaaagaagta ttatgagggc ttctttcgga
     1201 accgaccctc taatttttta gttttcatgt ttattttacc ttttttattc aggcagctca
     1261 acctccttac tgccatcctt atccttacag cagttcggcc aacttaacgg atcgcatgac
     1321 tgtttttctg tgatcccctt gttccaaaag caaattaatt gccgttgcct gccaatgttt
     1381 ggatacgatt gttccatcta tgagaaactt ttcgtcattg tcatgacttg ttacttcaca
     1441 tgcaacatag ccagattcgt ttttttgtag tatgtcttga attggcctga aaggagcgtt
     1501 ccctttttat acatttctca aatcagacat ttgcttttta ttgtgcttta ttttatatta
     1561 attataacaa tttgggcttt atatttttcc aatgttcact taaggtccaa tttatagtta
     1621 attttataaa agggcggtgt aggtatgagc gaaaacaata aatttaagga atcacttctt
     1681 gacaagaata caatgtcgat tacatgggaa ctggttcctg gaagaggtgc tcacgaaaaa
     1741 gaacaaaggg acgttatact aaatgctgaa aaagcttcaa aaggaggaaa aattcatgca
     1801 ctaactctaa ctgacaatcc gggtgggaac cctgcaattt caccagaaat actcgccgct
     1861 gaaatagtac aaatgggaat tgagcctttg gtgcacttta cttgcaaaga taaaaacagc
     1921 aatcagatag agagcttgct ttatgctcta gatcgggtgg gagttcggaa tcttcttgtc
     1981 atgaccggcg attatatgta ttccggtttt tttgccaagg ctaaacctgt atttgacctt
     2041 gaacctatgc atgtcttgac aatgatatct gccatgaatg aaggtttgga atataaggtg
     2101 cacaaaggaa ctaaaaaaca taagcctagc gatttttatg ccggtgttgt ttgttccccc
     2161 ttcaaaaaaa ccgaagcaga attgatgtgt cagtattaca aactgaaaat gaaagttgct
     2221 tctggcgcta aatttattgt cagtcagatt ggttttgatg ctagaaaaat tcatgaattg
     2281 attcaatttg tcaggctcaa caattgggat attcccgtaa ttggaaacat ttacgtcctt
     2341 tcatatggaa caggcagatt aatgaatcaa aataaaattc cagggtgtgt tgtaacgaat
     2401 aaattgctgg ctgagcttga agaagaaagc aaagctcctg ataaaggaaa aggagcaagg
     2461 ctgcttaggg cggctaaaat gtatacaata atgaaaggca tggggtatga tggagtccat
     2521 attggtggcc atggaacaaa gtatgaagat atagagtata taattgacaa gggcgaggaa
     2581 ctcgccaagg actggataag tttaattcct gaattcgact attcttttcc tgatggcttt
     2641 tactattttg aaaaagatga aaaaagcggc ctaaatactg ataaaattgc taatagaaaa
     2701 ggaaggaccc tggatgctaa agtaggtttt gtgtatcaca tgtcaaatct catccacgcc
     2761 cttatgctag aacctggcac tcctttatgg aagcccatga gagccatagc taatatgata
     2821 gatgacacag cactggaaag gccttatcat tttattgagc ggatgactaa gactatgctt
     2881 tacgattgta aggattgtgg cgactgtgca ctgccggaca tagcctattt atgcccaatg
     2941 tcacaatgcc ccaaaaacat cagaaatggc gcatgtggcg gaagttatga tggatggtgc
     3001 gaagtttacc ctggcaagaa aaaatgcatt tttgttagag cttatgcaag gcttaagtca
     3061 tccggcaaag aagaccatct ggaccacgaa gtaataccac catggaatgt cgatttaaat
     3121 cagacgtctg cttggataaa tctctattta ggaagagatc acaatgccaa aaagtatgga
     3181 gttaaaaaag ttgattctaa aaaacagtaa aaaagcgcca ctttttgacg ctgcttgtaa
     3241 gctatgggta aaaactatat aaaaatgtta atatgtttat cgaattttgc tatgtcccgg
     3301 gaaattatat ttataatctt catgaatata ctatttaaag gtaatgttaa atgccaaaaa
     3361 taaatataaa tacacccata gcttacactt attttttatt ctaaaatttt ttgataaaaa
     3421 aagtttttga gatttgaaaa accgtatttg tagggcatta ttatctgttc ggtgcttcca
     3481 acaaaaaata cacctctatc tgacagagca tcgtaaaact tcttataaag tttctccttc
     3541 gtatcttcgg taaaatatat catcacattt ctgcacaata ttaaatcaca accagttgga
     3601 taattatctt ctagtaaatt taagatttta aaattaacca tattcttaat atgatccttg
     3661 atgatatact tatcgtcttc ctgctcgaaa taagtcttta ccatagctga aggaatattg
     3721 gttattccct ttgctgtgta aaccccttgt tgtgcttttt caatagccac tcgatctata
     3781 tcgctagcta taatttcaac cctgctttta atcatcattt tatcaaaaat catggccaaa
     3841 gagtaaggct cctctcccgt tgaacatgca gaactccata cccttaatct ttttttgtat
     3901 tttaatagat ctggtatgat ttctcgttcc aacacctgcc attgggcagg attgcggaag
     3961 aattccgaaa catttattgt tatataatct aaaaactctt ttagatgatg tttgctcctt
     4021 tttaaaagct ctaaataact gtcataactt tcgtggccgt ttcgccttat aagggattct
     4081 attctccgct tcatttgctt ttccttgtaa tttgataaat ctatgttaca catttttttt
     4141 atttcttcag tgaaaccctt ataatccaat ttgcaacccc ctaataattt catattgcat
     4201 ataattataa ccaaagtgga caaaaaaatc catgatacat tatattttaa tgtatcatgg
     4261 gggctaaatg taaagggggt cttcagtatt ataatataac catattattg atttttatac
     4321 tatataaagc agaaaaatgc gcgactcttg ttcacgcatt tttcccgcta gacataatca
     4381 aaatcataaa acttcttact actcaaaata tctcctgcct ttataaattt atcaccttta
     4441 cgggctcatc aaacagacct cttttgactt cagttgcgtc catatgcttt gagccgagcc
     4501 cgtctgaaat gtaattacag gcatcccatg ggttaaccct gtcgccgcag gtgaaaacgt
     4561 caacagcggc atatcccagt tcaggccagg tatggatggc gaggtgcgat tcagatataa
     4621 ctactactcc gcttactcct tgaggactaa atttgtgaaa cgcaacctct cttacctcag
     4681 ctcctgcttc caaagctgct ttgaccaaga tttcttcgat taactccctg tcatttaaga
     4741 tgtttggatc gcaatcgtag acttcagcca gtatgtggcg gcccaaattt ttcatttgtc
     4801 catcaatacc cccttactat attttttcaa tcaaataaaa ttgtagcaga ttatcgcagt
     4861 ttgtcaatag aacacttaaa aatctttgct taaccctaaa aatatcagat tatctcaagc
     4921 taaattgaaa ttcctctata tttcatccaa tggtatgttt tcccttttat aatcacaggt
     4981 cgctttctta gttctgcaag gttactcgct ccggtcattg ccataacata ctttatctct
     5041 ttttctattt gcaatatatg tttttcaagc cctgaaggtc ctttgttata caaaatatgc
     5101 aaaatgcttc ctgcataagc cactgctttt gctccaagag caattgattt tgaagcatct
     5161 aaaccgtctt ttagaccacc agatgcgata acgtccgctt tactgccaac cgtcgttaaa
     5221 acttcaatca aacttattgg tgttggtatg ccccagtctt caaatttttt tagtggatta
     5281 gaccctcttc ttaatctttc tatagctata aagttactgc ctcctgttcc gcccacatca
     5341 ataattttta ctcccttttc taaaagctct accgcctcct ctcgggctat tccaaatccg
     5401 acttctttaa caataactgg aacccttaca tttcgaacaa tttcagctat attatcaacc
     5461 gtacctctaa agtctcggcg cccttccttc attatcactt cttgaggaac attaagatgt
     5521 agttgaagtg catctgcttt tatcatttct atagccatat tggcttcttc caccgtggca
     5581 tcagcaccta aatttgcaaa aattatacca tcaggattta attttcttac tatttcaaag
     5641 ctccttgcgc ttttcggatt gtccagtgct atcttttgtg agcccactgc catggctata
     5701 tttaatttct ttgcgattct ggaaagctga ctgttgatct tctcaccttc ttccatgcct
     5761 cctgtcatgg catttatgat tattgggctt gcaagcttta tatcttgtaa attcgttgaa
     5821 atatctattt cttttaaatt tacttcggat aaacagttgt gtaaaattgt tatatcctca
     5881 aactcatttc tttttaatct ttcttctaat aacaaactgt attttatatg ctcttttttc
     5941 ctcaactttc tgctttcttt cataatatgc ctcccagtaa acaaaatatg gggcgaattc
     6001 gccccaaatg caaaaattat tagattattt tttttcaaac aaatctccaa ccatttcacc
     6061 gatggtcaca ccattgtttt cgtttgtctt ttcatatacc tgcctattgt cattttcatc
     6121 ctgagcttct cttatgctaa ggctcattct cttttctttg gcattgatat ccataatctt
     6181 tacttttaca ttttcccctg tttttaaaac gtcttcagga tttgacacat ggtcttttga
     6241 tatttgtgaa atatgaacaa gaccc
//
LOCUS       DAONUO010000094         6219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9435_length_6219_cov_3.840526, whole genome shotgun sequence.
ACCESSION   DAONUO010000094 DAONUO010000000
VERSION     DAONUO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9435_length_6219_cov_3.840526"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PK768_04615"
     CDS             <1..729
                     /locus_tag="PK768_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQA60283.1"
                     /translation="SEREAKAIAEEQVNRQRESEFATSKKISLQQLYERLQKGEVKEL
                     NLIIKADVQGSIEALKQSLEKCSTNEVEVRIIHGAVGAVTESDVMLASASDAIIIGFN
                     VRPDSNARKLAEKEQIDIRTYRIIYDVVDDIKAAMKGMLEPEYEEVVLGRAEVRALFK
                     VPGVGVVAGCFVTEGKVTRNSKVRVIREGIVVYEGKIASLKRFKDDIREVVSGYECGI
                     GIEKFNDIKEQDVLEAFIDRPVQG"
     gene            755..1123
                     /gene="rbfA"
                     /locus_tag="PK768_04620"
     CDS             755..1123
                     /gene="rbfA"
                     /locus_tag="PK768_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQA60284.1"
                     /translation="MEFSRNERIAEEIKKAASLIINNELKDPRIDGLVSVTRVDVTRD
                     LRHATIFISFYGDNAKKNTTFDVIQKAKGFIRYELANQLRIKYMPEISIKLDESIEYG
                     FRIDKLLKDIKSQELSKEGN"
     gene            1125..2096
                     /locus_tag="PK768_04625"
     CDS             1125..2096
                     /locus_tag="PK768_04625"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HQA60285.1"
                     /translation="MDIARFYNLLENKLSFMITSHVSPDGDSIGSVLALTLALLKNEK
                     KAVPVIKDDIPRKYKFLPGTELIEKDISETFDVIIVLDCGDIERLGFDRQLKEYGPIV
                     VNIDHHKSNSYFGDLNLVDTTASSVGEMIYRLLDGRVELDYDIALNLYTSIITDTGSI
                     KYSNTTPLSIRILADLVDKGVRPDYVSRQVFEMRSPASIKLLKSVLSTLEISADGKVA
                     TLYITQEMLKTANADEDDTDGMINYAREIEGVEVAAIFRETDDSVIKVGLRSNEWVDV
                     SMIAAKFNGGGHPRAAGCQVKLPLKEAREIVLKAINHAMEDSGERRN"
     gene            2080..2973
                     /gene="truB"
                     /locus_tag="PK768_04630"
     CDS             2080..2973
                     /gene="truB"
                     /locus_tag="PK768_04630"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQA60286.1"
                     /translation="MKGVINVLKPPGMTSHDVVNFMRRLLQIKKIGHSGTLDPAAAGV
                     LPIFVGKATKAIEFFMDDDKEYIAEMRLGVTTDTGDLEGNVKDIRPIKVTEFDLKEAL
                     RQFTGEISQVPPMYSAVRYKGRKLYELAREGIVVERKPRRVKIYSLDLIDYSCESAIL
                     KVACSKGTYIRTLCEDIGKVLGCGACLSCLIRTSSGAFNIENSFTLEEIEQAKLYGRL
                     NEVILPVDEFLQKIPKVNLIAENAGFFTKGKKIQGDFKFLNDAELHLVRVYNHNKEFL
                     GIAKVIKDKDKISLQVIKSLN"
     gene            2990..3916
                     /locus_tag="PK768_04635"
     CDS             2990..3916
                     /locus_tag="PK768_04635"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQA60287.1"
                     /translation="MRVYSDLSSVKIPSYTVCGIGNFDGIHLGHQKLVQTILQYSKSN
                     KLDSLIFTFDPHPSTVLNPNNGAKLIMSSEKKRQIMESYGIDHYVQAPFTQEFSKIVY
                     TDFIHDILLDKCKAKIIIIGFNYRFGYKGLGTAEKLKELCSEVGVDTVIIPPVTYEGK
                     IVSSTFIRSLIEKGDVHSASKYLGRPFTIEGKVIRGKGLGKKLGFPTANIHFNRELVL
                     PAKGVYAVLVSWNGNFYKGVANLGNKPTFNGHDIVFEVHLFDFCSQLYGEKLEVSFVQ
                     KLRSETRFSKPEDLVRQVHKDMVCAKKILDAI"
     gene            4041..4310
                     /gene="rpsO"
                     /locus_tag="PK768_04640"
     CDS             4041..4310
                     /gene="rpsO"
                     /locus_tag="PK768_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQA60288.1"
                     /translation="MALVKEIKQSIINEYGIHDNDTGSPEVQVAILTERINQLTEHLK
                     KHKKDHHSRRGLLKMVGKRRALLNYLKDKDVERYKKLIEKLELRK"
     gene            4468..>6219
                     /locus_tag="PK768_04645"
     CDS             4468..>6219
                     /locus_tag="PK768_04645"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQA60289.1"
                     /translation="MFQYKTEVGGRNLIIETGKVAQQANGSVVIKYEDTVLLVTATAS
                     KEPREGVDFLPLTVDYEERLYAVGKIPGGFIKREGKPSEKAILSARLIDRPLRPLFPK
                     DFRNDVQIVATVLSVDQDNTPDIVAINGASCALCISDIPFDGPVGAVSVGLIDGKYII
                     NPTVSQSEKSRLNLTVAGTKEAVLMVEAGAHEVTEEEMLEAILFGHEEIKKIVEFQEE
                     IIKEIGKPKMQVQAEEVDAELERAVREYATDKILKAIKIYDKQEREAVIDEINEETLE
                     HFQELYPESEKQIANILYDILKEEVRNMITYEGIRPDGRRDDEIRPISCEVGILPRTH
                     GSGLFTRGQTQVLTVATLGALGDVQILDGLGIEESKRYMHHYNFPPYSTGETRVIRGP
                     GRREIGHGALAERALEPMIPPEEEFPYTIRLVSEVLSSNGSTSMASVCGSTLALMDAG
                     VPIKAPVSGVAMGLIKQDDKITILTDIQGMEDFLGDMDFKVAGTKKGITAIQMDIKIK
                     GIDENILKNALLRAKEARLFILDKMLSVISEPRKELSPYAPRIFSLTVDPEKIRDIIG
                     PGGKTINKIIAETNVKID"
BASE COUNT         2119 a          981 c         1379 g         1740 t
ORIGIN      
        1 agcgaaagag aagcaaaagc tattgcagaa gaacaggtaa atcgccagag agaatctgag
       61 tttgcaacat ctaaaaagat atccttgcag caattgtatg aaaggcttca aaaaggcgaa
      121 gtaaaggaac tgaatctaat cataaaagct gatgttcaag gttcaattga ggcactcaaa
      181 caatccctgg aaaaatgttc tactaatgag gttgaagtga gaattatcca cggagctgtt
      241 ggggcagtta cagaaagcga tgttatgctt gcttctgctt cggatgcaat tattattggt
      301 tttaatgtca gacctgattc aaatgcaaga aaactcgctg aaaaagagca aatagatatc
      361 agaacttata gaattattta tgatgtagtg gatgatataa aagctgcaat gaagggaatg
      421 cttgaaccag aatatgaaga agtggttctt ggaagggctg aagtgagggc gctgtttaag
      481 gtacctggag tcggtgttgt agccggatgc tttgttactg aaggtaaagt aactagaaat
      541 tcaaaggtcc gcgtcatacg tgaaggtatc gtcgtttacg aaggaaaaat tgcatccttg
      601 aagcgtttta aagatgacat tagggaagtt gtcagcggtt atgaatgtgg tattggaatc
      661 gagaaattca atgacataaa ggagcaggac gtgttggaag ctttcataga taggccagtt
      721 caaggataaa tactttcttg gggtgaattt agaagtggaa ttttctcgaa acgaaagaat
      781 tgctgaagag attaagaagg ccgcgagcct tattatcaat aatgaattaa aagacccgcg
      841 cattgatgga ttggtaagcg ttactagagt tgacgttact agggaccttc gccatgcgac
      901 tatatttatc agtttttacg gcgacaatgc aaagaaaaat accacctttg atgtaataca
      961 aaaggcgaaa ggtttcatca gatacgaatt agctaatcaa cttagaataa aatatatgcc
     1021 cgaaatatca atcaaattag atgagtctat tgaatacgga ttccggatag acaagctatt
     1081 aaaagatatt aagagtcagg aactatcaaa agaaggtaat taacatggat attgctagat
     1141 tttataatct cttggaaaac aaactaagtt ttatgattac atcacacgtt tcacctgatg
     1201 gtgacagtat aggttcagtc cttgcactca cattggcact attaaaaaat gagaaaaagg
     1261 ctgtaccggt tattaaggat gatataccga gaaagtataa atttctgccc ggaaccgagc
     1321 tcattgaaaa agatatttca gaaacttttg atgtgataat tgttctagat tgtggagata
     1381 tcgagcgatt gggttttgac agacaattaa aggagtacgg acctatagtt gtaaatatag
     1441 atcatcacaa gagcaacagc tattttggtg atttgaactt agtagatact actgcttctt
     1501 ctgtagggga aatgatatat cgattattag atggcagagt agaacttgat tatgacattg
     1561 cccttaacct ctatacttct atcattaccg ataccggttc aataaagtat tccaatacta
     1621 cacctctgtc cataaggatt ttggcagatt tagtagacaa aggtgtaaga cccgattatg
     1681 tcagtcgcca agtttttgaa atgcgtagcc cggcctctat taaactttta aaatcagttt
     1741 taagcaccct tgagatttca gctgacggta aagttgcgac tctatacatc actcaagaga
     1801 tgctcaagac tgctaatgct gatgaagacg atactgacgg gatgatcaat tatgcccgtg
     1861 aaatagaagg tgttgaagta gctgcaattt ttagagaaac tgatgattca gtgattaaag
     1921 taggtttaag atcaaatgaa tgggtggatg tcagcatgat agcagcaaag tttaacggag
     1981 gagggcatcc gagggctgcc ggttgccagg ttaaactccc cctaaaagaa gcccgtgaga
     2041 ttgtcttaaa agccataaat catgctatgg aggatagcgg tgaaaggcgt aattaatgtt
     2101 ttaaagccac ctggaatgac atctcatgat gtggtaaatt ttatgagaag gcttcttcag
     2161 ataaaaaaaa ttggccatag cggcaccctt gacccagcgg cggctggtgt cttacccatt
     2221 tttgtgggga aagctactaa agctatagaa ttttttatgg atgatgataa ggaatatatc
     2281 gcagaaatga gattaggtgt caccacagat acaggcgatt tagaaggaaa tgttaaagat
     2341 atcaggccga tcaaagttac cgagtttgat ttaaaagaag ctttaagaca gtttacgggt
     2401 gaaatctcac aggtacctcc aatgtactct gcggtgcgtt acaaagggag aaaattatac
     2461 gaattagcca gggagggcat tgtcgttgaa cgaaaaccca gaagggtgaa gatctactcc
     2521 ttagatttaa ttgattatag ttgtgaatct gcaattttga aagttgcctg ctcaaaaggc
     2581 acgtatatta gaacactatg tgaagacatc ggcaaggtcc tcggatgtgg agcttgtctt
     2641 tcctgtttaa ttaggacgag ttcaggcgct tttaatatag aaaactcttt tactttggag
     2701 gaaattgagc aagctaaact gtatggtaga ttaaatgaag taatattgcc agttgatgaa
     2761 tttttgcaga aaatacccaa agtaaatctc atagctgaga atgcgggctt ttttacaaaa
     2821 ggaaaaaaga tacaaggtga ctttaagttt ttaaatgatg ctgaactaca tttggtcaga
     2881 gtttacaatc acaataaaga gttcttggga atagcaaaag taatcaaaga caaagacaaa
     2941 atctcgttgc aagtaataaa atctttaaac taacaggatt gaggaaatta tgagagttta
     3001 cagtgatctt tcatctgtaa aaataccaag ttatactgtc tgcggcatag gaaattttga
     3061 cggcattcac ttaggtcatc aaaaacttgt gcagacaata ctacaatact ccaaatcaaa
     3121 taaactggat tccctaattt tcacctttga cccccatcca tcaacggttt taaatccaaa
     3181 taacggcgct aaactaataa tgtcaagtga aaaaaagaga caaatcatgg aatcatacgg
     3241 catagaccat tatgtccaag caccttttac ccaagagttt tcaaaaattg tctacacgga
     3301 ttttatacat gatattttgc ttgataaatg taaggctaag attataatta taggatttaa
     3361 ctatagattt gggtataagg ggctaggaac tgcggaaaag ctaaaagaat tgtgtagtga
     3421 agtaggagtt gataccgtaa tcattcctcc cgtaacctat gaaggtaaaa ttgtcagcag
     3481 cacatttatc agaagtctta tagaaaaagg cgatgtacat agcgcttcta aatatttagg
     3541 tcgtcccttt actatcgaag gcaaggtaat ccggggcaag ggtttgggca aaaaattagg
     3601 tttccctacg gccaatattc actttaacag ggaacttgta ttacctgcaa aaggcgtgta
     3661 tgcagttctt gtttcctgga atgggaactt ttataagggc gtggccaatt taggtaataa
     3721 accaacattt aacggtcatg atattgtatt tgaggtacat ttatttgatt tctgcagcca
     3781 gctgtatggt gaaaagctag aagtatcttt tgtccagaaa ctgcgttcag aaaccaggtt
     3841 ttcaaagcct gaggacttgg taagacaagt ccacaaagat atggtttgtg ccaaaaagat
     3901 tttagatgca atttaaattt acatttttgt gttgttgtga taaaattaat tgtaataaat
     3961 gaacctattg ctcggttact cgacgctccg acgaattact gggctactgg ggatttgtta
     4021 ataataagga ggtgagaaaa gtggcattag ttaaagaaat aaaacaatca attataaatg
     4081 aatatggaat ccatgataat gatacaggat ctcctgaagt tcaagttgcc atacttactg
     4141 aaaggataaa ccagttgacg gagcatttga aaaagcacaa gaaggatcac cattctcgca
     4201 gaggattact taaaatggta ggtaaaaggc gtgctctatt gaattactta aaagataagg
     4261 atgtagagcg ttataagaag cttatcgaaa aattagaatt aaggaaataa agcgggggtt
     4321 ccccgctttt tatgctgcct tgaaatgctt taatcctttt tatatcataa tacttagata
     4381 tgctttttag ctccaagaag gatataggtt tcttatgtag aataaaacta ttatttataa
     4441 aaaaactcaa agaaggagga ttgtttcttg tttcaataca aaactgaagt aggcggtcgc
     4501 aatttaatta tagaaactgg caaggttgct cagcaagcaa atggttcagt tgtgataaaa
     4561 tatgaagata ctgtcttgct agttactgct actgcttcta aagaaccccg cgaaggtgta
     4621 gattttttac cattaaccgt tgattatgaa gaaagattat atgctgtagg caagattccc
     4681 ggaggtttta ttaaaaggga gggaaagccc agcgaaaaag ctattctttc ggctaggcta
     4741 atagacagac ctttaagacc tttatttcca aaagatttta gaaatgatgt acagatagtt
     4801 gctacagtac tatctgttga ccaggataat acacctgata tagttgccat caacggagca
     4861 tcttgtgcat tatgtatatc cgatattcca tttgatggtc cggtaggagc agtgtccgtt
     4921 ggtctaattg acggaaagta tattattaat cctactgtat ctcaatcaga aaaaagtcgt
     4981 ttgaacctga ctgttgcagg cactaaagaa gcggtgctga tggttgaagc aggtgctcat
     5041 gaagtaacag aagaagaaat gttagaggcc attctattcg gccatgagga aataaaaaag
     5101 attgtagaat ttcaagagga gataataaaa gagatcggca agcccaagat gcaggttcaa
     5161 gcggaagaag ttgatgctga acttgaaaga gcagttcgag aatatgctac tgataaaatt
     5221 ttaaaagcga ttaagatcta cgataagcaa gaacgtgagg cagtaattga cgaaataaat
     5281 gaagagactc tagaacattt tcaggaatta tacccggaaa gtgaaaaaca aatagctaat
     5341 attttgtatg atatcttaaa agaagaagtt agaaatatga ttacctatga aggcataaga
     5401 ccagatggta ggagagatga cgagataagg ccaatttcct gtgaggtagg tattttgcca
     5461 aggacccatg gttcaggtct gtttacccgt ggccaaacgc aggttcttac cgttgctacc
     5521 ctcggtgccc ttggtgatgt tcagatatta gacggtcttg gtattgaaga gtcaaagaga
     5581 tacatgcacc attacaattt tccaccctat agcacgggcg agacaagggt aattcgtgga
     5641 cccggcagaa gggaaatagg tcacggtgca ttagcagaaa gagctctgga acctatgata
     5701 ccgccagagg aagaattccc ctataccata agactggttt ctgaggtatt aagttcgaat
     5761 ggttccactt ccatggcaag tgtttgcggt tccacattag cgctaatgga tgcaggagtg
     5821 cccataaaag ctcccgtttc tggtgtggca atgggattga ttaaacaaga tgacaaaatt
     5881 acaattctga cagatatcca gggaatggaa gactttttag gagatatgga ctttaaagta
     5941 gcaggaacca agaaagggat aactgcaata caaatggata ttaaaataaa gggaatagat
     6001 gagaatatac tcaaaaatgc attgctgcgc gctaaagaag ctcgcttgtt tatactagat
     6061 aagatgttat ctgtgatttc tgagcccaga aaggaacttt caccatatgc tccaaggatc
     6121 ttttctttaa ctgtagatcc ggaaaaaatc cgtgatataa ttggacctgg aggtaaaaca
     6181 ataaacaaga ttatcgcaga aaccaatgtc aagatagac
//
LOCUS       DAONUO010000095         6205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9464_length_6205_cov_4.173984, whole genome shotgun sequence.
ACCESSION   DAONUO010000095 DAONUO010000000
VERSION     DAONUO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9464_length_6205_cov_4.173984"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..377
                     /locus_tag="PK768_04650"
     CDS             111..377
                     /locus_tag="PK768_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQA60290.1"
                     /translation="MVQQNVIVKNKTGLHARPAALFVQAANKFKSEIFIEKEGKKVNA
                     KSIMGVMSLAVSQGTEITISAQGDDAQEAVDNLVELIESKFGEE"
     gene            442..1740
                     /locus_tag="PK768_04655"
     CDS             442..1740
                     /locus_tag="PK768_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HQA60291.1"
                     /translation="MTKHARIIEYIKNLKVGSKISVRQLANQLDVSEGTAYRAIKDAQ
                     LAGYVCTMPRKGTIRIEKKADEEIIHLSFAEVVNIVEGSVLGGKSGLHKPLSKFLIGA
                     MEVREMDKFIEPESLLIVGNRKDAQMLALKLGAAVLVTGGFGVDEEVIKLADKTGMPL
                     LSSSYDTFTVATIINRALYKRLVRKDVVRVKDAMVTEPDYLTVDSTVGDWRMLYKTTK
                     HSKFPVVDKDMKVCGVVTNNDIASLDDSVLIKDIMTKDPIVVTKDAPIAHATRLMVWE
                     DIKLIPVVENKRLVGILTKKDAIKAFQHLSFQPQIGETMDALVMNRFSMSKIENGVRL
                     TGKTDPLMLNPQGVASSGALMTLMANAGFEAFRVQKRLETVLDSFTVYFTKPVQLEQD
                     IEVEAIIIDMGRKSGKAEINIVHEGNLVAKSIISVRVIDR"
     gene            1750..5166
                     /locus_tag="PK768_04660"
     CDS             1750..5166
                     /locus_tag="PK768_04660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQA60292.1"
                     /translation="MAEFVHLHVHTNYSLLDGACEIDRLARRLSELGMKSCAITDHGA
                     MYGVIDFYKKMLAYGIKPIIGAEVYMAERIMQDKEPGVDDEQYHLVLLAKDIQGYKNL
                     MKLVSLGFTEGFYYKPRIDMDILAKYSEGLIALSGCLAGRIPSLLLKGNFDEAKNIAL
                     QLNSIFGQDNFFIEVQDHGLLDQRRIINDLIRLSQETGIPLVATNDVHYIQKEDALAQ
                     DVLMCIQTGKTLDEENRMKFESSEFYLKSPEEMASLFSYIPEALENTIRIAESCNVTL
                     DFGTIHLPSFRVPEGLTEDEYLNRLCYKGARERYPEITGEIKQRLDYELETIKKMGYS
                     SYFLIVWDFINFARQNGIMVGPGRGSAAGSLVAYCLYITNIDPLKYNLLFERFLNPER
                     ISMPDIDVDFCYERRQEVIDYVVRKYGQDRVAQIVTFGTMAARAAIRDVGRVMGYPYG
                     EVDRIAKMVPAELGITIENALTLNPELKKLYEENERIRRLIDIAKDLEGFPRHASTHA
                     AGVVISKDPIVEHVPLHKLGDSNVTTQYTMTALEELGLLKMDFLGLRTLTVIRDAINI
                     IRRTKNVEINLDKLPLDDKKVYEMLSQGNTAGVFQLESTGMRNLLKELRPETFQDVAA
                     IIGLYRPGPLGSGAAEDFIKSKNGLKPIKYLHPKLEPILSETYGIILYQEQAMKIAQE
                     LAGFSLAQADILRKAMGKKQQDVMAAQRESFVNGCVKNGIDKVTAEKIFDEISYFAGY
                     GFNKAHSAAYAVIAYQTAYLKAHFPVEYMAALLTSVIDNSDKVAHYINECRHLGIKVL
                     PPDINESYESFTVVSDKIRFGLTAVKNVGHNVARAIIMARKSEGKFTSFTDFVEKVSG
                     DLNKKALESLIKSGAFASIGAKRSQLLAIYEDTLTRIQKEQRQGMKNQISLFQILDDT
                     ASYGESLPDVPEFPSAELLAMEKDMLGIYLSGHPLSEFERELSENVTFYLGDKNNDNI
                     DKKEVLVGGIITGIQTKATRNESIMAFINIEDLTGSMEAIVFPTVFEKYKSLLHPDSK
                     VYIKGRIDSKEDEAPKLLVEEIRPLGKRESEGTVVFTVSNGDQLDMNALKDFISNHMG
                     NDRVVIYIKETAKSYLLDKKMEASRENLNMLASITGLRNCTFWDKLIVKN"
     gene            5217..5411
                     /locus_tag="PK768_04665"
     CDS             5217..5411
                     /locus_tag="PK768_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60293.1"
                     /translation="MWTVIYMATDKDTAHKVVQAIKDEGFLVKTREVMKSKKHGCCIE
                     ISVPESEAQEAQNVILEKNL"
     gene            5435..>6205
                     /gene="pfkA"
                     /locus_tag="PK768_04670"
     CDS             5435..>6205
                     /gene="pfkA"
                     /locus_tag="PK768_04670"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQA60294.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAIVRKAVYYGIEVYGVHRGYMGL
                     INSDFVKLDISSVGDIIHRGGTVLLTARCDEFKTKEGQEKALKNLEAFGIEGMIVIGG
                     DGSLRGANVLSKMGVPTIGIPTTIDNDIAYTDNCIGFDTAVNTVVDALNKVRDTATSH
                     ERTFVIEVMGRKSGHLALHTGLASGAETILVPEIPFDLDAVCEKIKSGYKRGKLHSII
                     VVAEGAASSLEVGKVIKEKTNLETRVIILGHLQRGGNPT"
BASE COUNT         1943 a         1247 c         1537 g         1478 t
ORIGIN      
        1 atttttacca atcatatttt ctttatcaat cacatccgga gtgatttata gattaaaaaa
       61 gattgaagaa atggctaaca aattacagtc tatgaaaggg gatttatgaa atggttcaac
      121 aaaatgtgat tgttaaaaac aaaacagggc ttcatgcaag acctgcagca ctcttcgttc
      181 aggcggcgaa caagtttaaa tccgaaatat ttattgaaaa agaggggaaa aaggttaacg
      241 caaaaagtat aatgggcgtt atgtccctag cagtcagcca aggaactgag attacaatat
      301 ctgctcaggg tgatgacgca caggaagccg tagacaatct agtggaatta atcgaaagca
      361 aatttggaga agaataaatt taaaagctac cgtgacggta gcttttaaat taggtataat
      421 taactaaagg tgagttaaat tatgacgaaa catgcccgta ttatcgaata tatcaaaaac
      481 ttaaaagtag gctcaaaaat ctcggtcagg cagttggcaa accaattaga tgtcagtgaa
      541 ggcactgcat atcgagcgat taaagacgcc cagcttgccg gctatgtgtg caccatgccc
      601 agaaaaggta ccatccgcat cgaaaaaaag gcggacgaag aaatcatcca cttatccttt
      661 gcggaagtag tcaacattgt agagggcagc gtgttgggag gcaaatccgg acttcacaag
      721 ccccttagca aattcctgat tggggccatg gaagtaagag aaatggacaa gttcatcgag
      781 ccggaaagcc ttttgatagt cggcaatcgc aaggacgcac aaatgctggc cttaaaactg
      841 ggtgctgcgg tgctcgtgac aggtggtttt ggtgtagatg aggaagttat aaaacttgca
      901 gacaaaaccg gaatgccgct gctttcttcg tcctacgaca cctttacggt ggcaacaatc
      961 ataaacaggg cgctgtacaa gagattggtg agaaaagacg tagtcagagt caaggatgca
     1021 atggttacgg aacccgatta tttgaccgta gattccacag tgggcgattg gcgaatgctg
     1081 tacaaaacca caaaacacag caaatttccc gtggtggata aggatatgaa agtctgcggc
     1141 gtcgtaacaa ataatgacat tgccagtcta gatgacagtg ttttaataaa ggatattatg
     1201 accaaagacc caattgttgt cacaaaagac gcgcctattg cccatgccac acgattgatg
     1261 gtgtgggagg acattaagct gataccggta gttgaaaaca aaaggctggt aggtattctg
     1321 acaaaaaagg atgcaattaa agccttccaa cacttgagct ttcaaccgca aatcggagaa
     1381 accatggacg ctttggtcat gaaccggttt tccatgagca aaatcgaaaa tggagtgagg
     1441 ctcacgggca agacggatcc cttaatgctc aatccccagg gtgtagcaag cagcggagcc
     1501 cttatgacct taatggcaaa tgccggcttt gaagcattta gggtgcaaaa acggcttgag
     1561 acggttttgg acagttttac ggtgtacttt acaaaaccgg tccagttgga acaggatata
     1621 gaagttgaag ccataataat cgatatgggt agaaagtcgg gcaaggccga aataaatata
     1681 gtacatgaag gaaatctggt agcaaagtcc attatttccg ttagggtaat cgacaggtag
     1741 gtgatttttg tggcagaatt tgtccacttg catgttcaca caaattatag cctcttggac
     1801 ggcgcctgtg agatagacag gcttgccagg cggctttcag agctgggcat gaaaagctgt
     1861 gccattaccg accacggtgc aatgtacggt gtgatagatt tttacaaaaa aatgctggcc
     1921 tacggaataa aaccgattat aggtgctgag gtgtacatgg ccgagcggat catgcaggac
     1981 aaagagcccg gcgtggatga tgagcagtat catctggtgc ttttggccaa ggacattcag
     2041 ggctataaaa acctgatgaa gcttgtttca ctgggcttta cggaaggttt ttactacaag
     2101 cccaggattg atatggatat tttagctaaa tactcggaag gcctgatagc acttagcggc
     2161 tgccttgccg gaagaattcc gtcacttctt ttaaagggca attttgatga ggccaaaaat
     2221 attgccctgc agcttaattc aatattcggg caggataact tcttcataga agtccaggac
     2281 cacggcctct tagaccagcg aagaattata aacgacctga ttcgcctgag ccaggaaacg
     2341 ggaattcccc ttgtagccac aaatgacgtc cactatattc aaaaagagga tgccctggcc
     2401 caggatgtgc tgatgtgcat acagacgggg aaaaccctgg acgaggaaaa ccgaatgaaa
     2461 tttgaaagtt cagagtttta tttaaagtcg ccggaagaaa tggcgagcct cttttcatat
     2521 attcccgaag cccttgaaaa taccatcaga atagccgaga gttgtaacgt gaccctggat
     2581 tttggaacca tacacctgcc gagcttccgg gtcccggaag ggcttacgga ggatgaatac
     2641 ttaaatcggc tctgttacaa gggtgcgaga gagagatatc ccgaaataac tggggaaata
     2701 aaacagcggc tggactacga acttgagacc ataaaaaaga tgggttattc cagttatttt
     2761 ttaattgtct gggattttat caattttgcc aggcaaaatg gcataatggt gggacccggc
     2821 aggggatctg ccgccggcag cttggtggca tactgcctat atataaccaa catagacccg
     2881 cttaagtaca acctcctctt tgagcgcttt ttaaatcctg aaagaatttc tatgcccgat
     2941 atagatgtag acttttgtta tgagcgccgg caggaagtta tagactacgt ggtccgaaag
     3001 tacggccagg acagggtggc tcaaatcgtg accttcggca ccatggcggc cagggctgcc
     3061 atacgtgacg tagggcgggt aatggggtac ccttacgggg aagtagacag gattgcaaag
     3121 atggtccctg ccgagcttgg aatcaccatt gaaaatgccc tcactttaaa cccggagcta
     3181 aaaaagctgt atgaggaaaa tgaaaggata aggcgactta tcgatatagc caaggaccta
     3241 gaaggttttc cccggcacgc atcaacccat gcggcgggag ttgtaatatc gaaggacccg
     3301 attgtggagc atgtcccctt acataaactg ggcgattcaa atgtgacaac ccagtatacc
     3361 atgacagccc ttgaagaact ggggcttttg aaaatggact ttttgggtct tcgaaccctt
     3421 acggtcataa gggatgccat aaatattata cgccgcacca agaatgtgga aataaacctg
     3481 gacaagcttc ccttggatga taaaaaagta tatgaaatgc tcagccaggg aaataccgcc
     3541 ggggtatttc agttggaaag caccggcatg agaaacctgc ttaaagaatt aagaccggaa
     3601 accttccagg acgtagcagc aattatcggt ctgtaccgcc cggggcccct cggcagcggt
     3661 gctgcggaag attttatcaa aagcaaaaac ggtctaaaac ccatcaagta cctgcatcct
     3721 aagcttgaac cgatattatc cgaaacctac ggtataattc tctatcaaga acaggccatg
     3781 aaaattgccc aggaactggc cggcttttca ctggcccagg cagacatttt gagaaaggcc
     3841 atgggcaaaa agcagcagga cgtaatggct gcccagagag aatcttttgt aaacggctgt
     3901 gtgaaaaacg gcatagacaa agttactgca gaaaaaatat ttgacgaaat ttcctatttt
     3961 gcaggttacg gttttaacaa ggcccattca gccgcctatg ccgttatagc ctaccaaacg
     4021 gcatatctta aggcccactt ccccgtcgaa tacatggcgg cgctgcttac cagtgtcata
     4081 gataattccg ataaggttgc ccactatata aatgagtgca gacacttggg cataaaagtt
     4141 ttacctccgg atattaacga gagttacgaa agcttcacgg tagtgtcgga taaaatacgc
     4201 ttcggcctta ctgctgtaaa aaacgtgggc cacaatgtgg caagagctat tataatggcc
     4261 aggaaatccg aaggcaagtt cacgtctttt acggattttg tggagaaggt atccggagat
     4321 ctcaacaaaa aagccctgga aagtcttata aaaagcgggg cttttgcgtc aatcggggca
     4381 aagcgctcgc agctcctcgc catttacgaa gataccctga cgaggattca gaaggagcaa
     4441 agacagggta tgaaaaatca aatctctctt ttccaaatac tggacgacac tgcaagttac
     4501 ggggaaagct tacccgacgt ccctgaattt ccttccgctg agctcctggc catggaaaag
     4561 gacatgctgg gcatatacct cagcggccat ccactttccg aatttgaaag ggagctttcg
     4621 gaaaatgtca ccttttacct tggggataag aataacgaca atatagataa aaaagaagtg
     4681 ctcgtaggtg gtatcataac cggaatacaa acaaaagcca ccaggaatga gagcatcatg
     4741 gcctttataa atatagagga cctgacgggg agtatggaag ctatagtgtt cccaacggtt
     4801 tttgaaaaat acaaaagtct tttgcatccc gattccaagg tctatattaa aggcaggatc
     4861 gattcaaaag aagatgaagc gccaaaattg ttggtggaag aaataaggcc tttggggaaa
     4921 agggagtctg aaggtacggt tgtttttacc gtctcaaacg gggaccagct ggacatgaat
     4981 gccttaaaag atttcatttc caatcatatg ggcaatgatc gggtcgtcat atatataaaa
     5041 gaaacggcca aaagttatct gcttgataaa aaaatggagg cttctaggga gaatttaaac
     5101 atgcttgctt caataactgg cttgagaaat tgtacatttt gggataaatt aatagttaag
     5161 aactagtgct atttgcaacg ccttatataa taccggaata aagggggccg attaatttgt
     5221 ggacggttat atatatggcc actgataagg atacagctca taaggttgta caagccataa
     5281 aagatgaagg tttcctggtg aagacgaggg aagtcatgaa aagtaaaaag catggctgct
     5341 gtattgaaat tagtgtccca gaatctgaag ctcaagaggc acaaaatgtc attctagaga
     5401 aaaaccttta accggaaagg agcaatataa attcatgaag agaattggaa tactgaccag
     5461 tggtggagac gcacctggaa tgaatgcagc tgtcagggcg atagtcagaa aagccgtata
     5521 ttacggcata gaagtttacg gcgtgcatag aggctatatg ggactgatta attccgactt
     5581 cgtcaaactg gatatcagct ccgtaggcga tataatacac cggggcggaa cggtgctgct
     5641 caccgccagg tgtgatgaat tcaaaacaaa agaaggccag gaaaaggcct taaaaaatct
     5701 ggaggctttc ggcatagagg gcatgattgt cattggaggt gacggctcct taagaggagc
     5761 aaatgttttg tctaagatgg gtgttcctac tatcggtatt ccaactacca tcgacaatga
     5821 tattgcctat accgacaact gcataggatt tgataccgca gtcaataccg ttgtcgatgc
     5881 cttgaacaag gtcagggata ccgccacatc ccatgaaaga acttttgtca ttgaggttat
     5941 gggcaggaag tccggccatt tggcccttca taccggcctt gccagcggtg cggaaacgat
     6001 cttggtgccg gaaataccct ttgatttaga tgctgtctgc gaaaaaatca aatcaggcta
     6061 caagagagga aagctgcaca gcattatagt tgttgctgaa ggtgcagcca gtagcctgga
     6121 agtaggcaag gtgattaagg aaaagaccaa tctggaaaca agggttataa ttcttggcca
     6181 cctgcaaagg ggtggcaatc ccact
//
LOCUS       DAONUO010000096         6204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9468_length_6204_cov_2.946658, whole genome shotgun sequence.
ACCESSION   DAONUO010000096 DAONUO010000000
VERSION     DAONUO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9468_length_6204_cov_2.946658"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PK768_04675"
     CDS             complement(<1..735)
                     /locus_tag="PK768_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HQA60295.1"
                     /translation="MTDYKLTDVIDKVKLREMLAKFTEATDLAAIPCDLTGKNITEPT
                     NFTRHCHMVRSTEIGKKLCYASDEEHGRISMEQGKPVIFPCHCGLMDLAAPIVVEEKC
                     YGYILCGQVFLEPPEAEDIRKARLRAKKFGLDEDEYVQSFLEVKVFTRERLIAATEML
                     NIFSNYIVELGINNLMQQRLLEEERLRMQLENTLHSLELKVLQAQMNPHFLFNTLNTA
                     ARLSYLENAKRTGEIIYSLASLLRYSL"
     gene            complement(869..1276)
                     /gene="mgsA"
                     /locus_tag="PK768_04680"
     CDS             complement(869..1276)
                     /gene="mgsA"
                     /locus_tag="PK768_04680"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HQA60296.1"
                     /translation="MNIALIAHDAKKDQMVDFTIAYEDILKEHTLFATGTTGKRIMDA
                     TGLKVKRLQSGPLGGDQQIGAMVAEETMDLVIFLRDPLTAQPHEPDINALLRVCDVHN
                     VPLATNLATAEVLIKGLMRGDLDWRKVVNPTLS"
     gene            1442..2494
                     /locus_tag="PK768_04685"
     CDS             1442..2494
                     /locus_tag="PK768_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQA60297.1"
                     /translation="MTVTLDDFILNLLSNPALVITVILTLGTITVNGWTDAPNAIATC
                     ISTRSISPQGAIIMAAIFNFLGVFVMTTYNSKVAETIYNMVDFGGDAHYALIALCAAM
                     FAIVVWATAAWWFGIPTSESHALIAGISGAAIALQGGIQGINFDEWVKVIYGLGLSLF
                     LGFFSGWLVVKIVEYIFRGSDRRKTYGFFKNAQILSAAGMAFMHGAQDGQIFMGVFML
                     GVFLARGQASVTEFVIPFWLIVLCSLVMAMGTSLGGMRIIKSVGMDMVKFETYQGFSA
                     DLAGVICLFLASVFGLPVSTTHTKTTAIMGVGAARRISSVNWGIVKEMIIAWVLTFPG
                     CGIISFLLAQVFMRLF"
     gene            2528..3148
                     /locus_tag="PK768_04690"
     CDS             2528..3148
                     /locus_tag="PK768_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQA60298.1"
                     /translation="MRKRDNYYFDTFVELVDYSCKAADLLNEIVSNYDATNIKAKMEE
                     MHSIEHSADVARHKMMGRLLKEFITPIDREDIMAMADAIDTVTDSIEDVLMRLYMYNI
                     IEVTEHAQKLAEIIAKCCYLLKQAFEELPNFRKSKKLHDLIVEINRLEESGDALYTEA
                     IRDLYLSSKSYKEIVAWDNTYHYMEKCCDTCEDVSDVIENIIMKNT"
     gene            complement(3267..4115)
                     /locus_tag="PK768_04695"
     CDS             complement(3267..4115)
                     /locus_tag="PK768_04695"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: protein motif:HMM:NF009688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HQA60299.1"
                     /translation="MYEKAMPDNLSIQEKLRLSKETGFDYLEISIDETDEKLARLDYT
                     KQELLKIKHAMEAESFHIKTMCLSAHRRYPLGSHEKSKRDKSIEIMGKALEFSGELGI
                     RIIQLAGYDVYYEKSDEKSKEFFLENLRKVTDMAASYGIVLGFETMETEFMNTISKSM
                     FYVKYINSPYLGIYPDIGNLKNACVLYNIDITKEINAGRGHIFAAHLKETAPGIFRNM
                     DFGTGHTEYTTCIEKLLEQGVRMFTGEFWHQGEKNYREIIKNANEFLWNKVNEAMQRA
                     KLTIHN"
     gene            complement(4137..4835)
                     /locus_tag="PK768_04700"
     CDS             complement(4137..4835)
                     /locus_tag="PK768_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HQA60300.1"
                     /translation="MLDELKLQVFEANLLLPKHNLITFTWGNVSGIDRSRKYIVIKPS
                     GVEYEEMAVDDMVVLDIDGNVVEGELRPSSDTPTHLELYRNFDNIWGIAHTHSKWATI
                     MAQSKMDIPALGTTHGDYFYGDIPCTREMTREEINGEYELETGKVIIETLHKRRINPM
                     KIPAVLVANHGPFVFGNSPEEAVRNSVILEEIAFMAWHGIAIAKDERMQQDLLDRHYL
                     RKHGKDSYYGQISG"
     gene            complement(4837..5292)
                     /locus_tag="PK768_04705"
     CDS             complement(4837..5292)
                     /locus_tag="PK768_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60301.1"
                     /translation="MGKLTPYQNYKEWNIEHIIKKPISDIGICDIDGDGIDELATIEP
                     FHGAKFRIYKKIDGEYKAIYEYPNETEFLHVVWGGQIRGKKTFIGGARRKDAELFMVQ
                     YRDGEYETVILDKGRGPANISVVNGVNKDFIITANNSIHEACVYIVEDD"
     gene            5310..5507
                     /locus_tag="PK768_04710"
     CDS             5310..5507
                     /locus_tag="PK768_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60302.1"
                     /translation="MNQLMLTRFTEGVIVVAESGKLGINNTFISGNISDITMLITDCD
                     ADRRFIEKLEDMGVRVILTEY"
     gene            complement(5579..6034)
                     /locus_tag="PK768_04715"
     CDS             complement(5579..6034)
                     /locus_tag="PK768_04715"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HQA60303.1"
                     /translation="MMDVPVLAAHMFVFYFGIIADVTPPVALAAFVGAGIAKANPLKT
                     GIQATKLAIAAFLVPYIFVYNPSLLLIDVNAVDMILMSITAIVGIIGVGAGMEGFFLM
                     DLTVIERVIFFVGGLLLVIPGLQTDIVGFSLLAIGYIMQRRKAALLKVQ"
     gene            complement(6031..6168)
                     /locus_tag="PK768_04720"
     CDS             complement(6031..6168)
                     /locus_tag="PK768_04720"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HQA60304.1"
                     /translation="MVDIADGKLFLTLVFTMLTSIILGMGVPTTANYVITSTIAAPAL
                     I"
BASE COUNT         1745 a         1368 c         1156 g         1935 t
ORIGIN      
        1 gagggaatat cttaaaagac ttgccaggga ataaatgatc tctccggtcc gctttgcatt
       61 ttcaagatat gaaagtctcg cagcagtgtt taaagtgtta aataaaaaat gggggttcat
      121 ctgtgcttgt aaaactttga gttccaggga atgcagggta ttttccagct gcattctgag
      181 tcgttcttcc tccaagagcc tttgctgcat gaggttgttt atccccaatt caactatgta
      241 gttggagaag atgtttaaca tctccgtagc agcgatcagc ctttctctcg tgaaaacctt
      301 tacctccaga aaactctgga cgtattcgtc ttcatctaag ccgaactttt tagcccttag
      361 ccttgccttc ctgatatctt ccgcctcggg aggttccagg aagacctggc cgcacagtat
      421 ataaccgtaa cacttttctt caaccacaat gggagccgcc aaatccatca atccacaatg
      481 gcaggggaag attaccggct tgccctgttc catggatatt cggccgtgtt cttcatccga
      541 ggcgtagcac agcttttttc cgatctccgt ggagcgcacc atgtgacagt gtcttgtaaa
      601 gttagtaggc tccgtgatgt tttttcccgt caggtcacag ggaatggccg ccaaatccgt
      661 tgcttcagta aactttgcca gcatctccct taattttacc ttgtcaatga catcggttag
      721 tttgtaatca gtcatgtcaa accccctaaa ggaaattaac ttgcaaaaag agttttactt
      781 gttattttat tataatatat ttgacttgta aaaataaaga aaaagacatg gttttgggca
      841 aaaatgtacc atgtcttttc tcaactcttt agctcaatgt ggggtttaca acctttctcc
      901 agtcaaggtc accgcgcata agtcctttta tcaatacttc tgccgtggcc agattggttg
      961 ccaagggcac attgtggaca tcgcacaccc tgagcagcgc atttatatcc ggttcgtggg
     1021 gctgggcggt taagggatct ctcaagaata tcacaaggtc catggtttcc tcggcaacca
     1081 tggcgccgat ctgctggtcg cctcccaagg gccccgactg taggcgcttt acctttaggc
     1141 cggtagcatc cattatcctc ttgcccgtcg tgcccgttgc gaaaagggta tgttccttta
     1201 aaatatcctc ataggctata gtaaaatcca ccatctgatc tttctttgca tcgtgagcta
     1261 taagggcaat attcatataa aaacctccaa attaagcaat ttttgtgctt actgcattta
     1321 tcttactata ttatgtttgc ttttttcaat aatatctgta taattatatt gtgcaattgt
     1381 tcactaataa tgtgaaaact ccttattatt ttttcataaa aaatatttgg aagatgatga
     1441 tatgactgtt actttggatg attttatact aaatttactg tctaacccgg ccctggtcat
     1501 tacggtaatc ctgacattgg ggaccattac ggtcaacgga tggaccgacg ccccaaatgc
     1561 catcgccacc tgcatttcta cccgctccat cagtccccaa ggcgccataa ttatggcagc
     1621 catatttaac ttcctgggcg tattcgtcat gacaacgtac aactcaaagg tggcagaaac
     1681 catttacaac atggttgact ttggaggcga tgcccactat gctttaatcg ccctttgcgc
     1741 tgccatgttc gccatcgtgg tatgggcaac cgctgcctgg tggttcggca tacccaccag
     1801 cgaaagccat gctctcattg ctggaatcag cggtgctgcc attgctctgc agggcggcat
     1861 acagggaata aattttgacg agtgggttaa ggtaatatac ggcctgggat tgtccttgtt
     1921 tttaggattt ttttcgggct ggctcgttgt caagatcgtg gaatacatat tcaggggttc
     1981 tgaccgcaga aagacatacg gattctttaa aaatgctcaa atactcagtg cagcaggcat
     2041 ggcctttatg cacggtgccc aggacggcca aatattcatg ggtgtcttta tgctgggcgt
     2101 atttctcgcc cggggacagg cttccgttac agaattcgtg atacccttct ggctgatcgt
     2161 gctctgttct ttggtcatgg ccatgggaac ttccttaggc ggaatgcgca taattaaatc
     2221 cgtgggaatg gatatggtaa agtttgaaac ctaccagggc ttttccgctg acctggccgg
     2281 agttatttgc ttgtttctcg cttccgtctt tgggcttccg gtcagcacca cgcacaccaa
     2341 gacaaccgcc ataatgggag taggtgctgc gagaagaata tcctcagtaa attggggaat
     2401 agttaaagaa atgattatcg cctgggtttt gacctttccg ggatgcggaa ttattagctt
     2461 tttgctggca caagttttta tgagattatt ttaatataaa aacatataga ttggggaagg
     2521 catgattatg cgcaaaagag acaattatta ttttgacacc tttgttgaat tggtggatta
     2581 ctcctgcaaa gctgcagacc tgttaaatga aatcgtctca aactacgatg ccacgaacat
     2641 aaaggcaaag atggaagaaa tgcacagtat cgagcacagc gcggacgtgg caaggcacaa
     2701 aatgatggga agacttctca aagagttcat cacacccatt gatcgcgaag acatcatggc
     2761 catggcagat gccatcgaca cggtaaccga cagcatagaa gacgtattga tgcggctgta
     2821 catgtacaat atcattgaag ttacggaaca tgcccaaaag ttggccgaga ttattgcaaa
     2881 gtgctgttat cttctaaaac aggcctttga agaactgccc aatttccgca aatccaaaaa
     2941 acttcacgat ctaatcgttg aaatcaaccg cctggaagaa agcggagatg ccctttatac
     3001 cgaagcaata agagatcttt acctcagcag taaatcttat aaggaaatag ttgcctggga
     3061 taatacgtat cactacatgg aaaaatgctg tgacacctgt gaagacgtgt ccgatgtcat
     3121 tgaaaatatc attatgaaga atacctaaaa atggaattaa agggacatat gtttttaaaa
     3181 tgtggaaggc ttaactagac gttaatctta ttctcggaat ttggctactc taaaaacggc
     3241 ttaggggaat tataccccta agccgtttaa ttatgtattg ttagctttgc tctttgcata
     3301 gcttcattta ctttattcca taaaaactca ttagcatttt taattatctc gcgataattc
     3361 ttttcacctt gatgccagaa ttccccggta aacatcctca caccttgttc taataacttt
     3421 tcaatgcaag ttgtgtactc tgtatgacct gttccgaaat ccatatttct aaatatgcct
     3481 ggagcagttt ctttcagatg ggcagcaaat atatgacctc tacccgcatt tatctcctta
     3541 gttatgtcta tattatacaa aacacaagca tttttcaaat tgcctatgtc cggatagatg
     3601 cctaaatatg gcgaatttat atacttaaca tagaacattg actttgaaat agtattcata
     3661 aattcagttt ccatagtctc aaagcccaaa actataccat aagatgccgc catatcagta
     3721 actttcctta ggttttctaa aaagaattct ttactttttt catcactttt ctcataatag
     3781 acatcatatc cagcaagttg aataatccta atgcctaact caccggaaaa ctccagggct
     3841 tttcccatta tttcaatact tttgtctctt ttgctttttt catggctacc aagcgggtac
     3901 cttctatgag cacttaaaca catggttttt atgtgaaaac tctctgcttc catagcatgt
     3961 tttattttca acaattcttg ttttgtataa tcaagtctcg ctaatttctc atctgtttcg
     4021 tctatactta tttccaaata atcaaaacca gtttctttag ataatctgag tttttcctgt
     4081 atacttaaat tatccggcat tgctttttcg taaattccaa tatttacccc atttcttcac
     4141 ccacttatct gcccatagta agaatcctta ccatgctttc gcaaataatg tctatctaat
     4201 aaatcttgtt gcattctttc atcttttgcg attgctatac catgccatgc cataaaggct
     4261 atttcttcta atattactga attccgaaca gcttcttccg gagaatttcc aaaaacgaaa
     4321 ggcccatgat ttgcaaccaa cactgcaggt attttcatag gattaattct ccgtttatgc
     4381 aaagtttcaa ttataacctt acctgtttca agttcatatt ctccatttat ctcttcccta
     4441 gtcatttctc ttgtacaagg aatatctcca taaaaataat ccccgtgagt tgtcccaagt
     4501 gccggtatat ccattttact ttgggccatt atagtggccc atttcgaatg ggtgtgcgca
     4561 attccccata tattatcaaa gtttctatat aattctaagt gggttggtgt gtcacttgac
     4621 ggccttagtt ctccttcaac tacattgcca tctatgtcaa gtacaaccat atcatctact
     4681 gccatctctt catactctac tcctgaaggt ttaatcacaa tatattttct actcctgtct
     4741 atgccggaaa cattacccca tgtaaatgtt ataagattat gcttaggcaa caataaattt
     4801 gcttcaaaaa cctgcaactt taattcgtct aacacatcaa tcatcctcca caatatatac
     4861 acaggcttca tgaatgctgt tatttgcagt tataataaaa tctttattaa caccattaac
     4921 aactgaaata ttggcaggcc ctcttccctt atctagaata acagtttcat attcgccatc
     4981 tctatactga accatgaaaa gttctgcatc tttccttctg gcaccaccta taaaggtttt
     5041 tttccctcta atttgacctc cccaaactac atgcaaaaac tctgtttcat ttggatattc
     5101 ataaattgct ttgtattcgc cgtcaatttt tttataaatc ctaaacttcg caccatgaaa
     5161 tggttctata gttgccagtt catctatccc atcgccatct atatcacaaa tccctatatc
     5221 gctaattggc tttttgatta tatgttcgat gttccattct ttatagtttt gatatggggt
     5281 taacttcccc aaatttagat gaagtagctg tgaatcagct catgttaacc agatttacag
     5341 aaggtgtaat agtagtggct gaaagtggca aattaggcat taataatact tttatatcgg
     5401 gaaatatttc agatattacc atgttgataa ccgactgtga tgcagataga agatttatag
     5461 aaaaattaga agatatggga gtgagggtta tattaacaga gtactgatac agaaaactta
     5521 ataaaaaaga aaaaggccta gaggaatcta tcttcctcta agcctttttt tgtcgtattt
     5581 attgtacctt aagcagtgca gcctttcttc tttgcattat atatccgata gccaaaagcg
     5641 aaaagcccac tatatccgtt tgaagtcccg gaatcacgag caataagccg cctacgaaaa
     5701 agattaccct ttcaataacc gttaaatcca ttaggaagaa tccttccata cctgctccta
     5761 ctccaataat accgacaatt gcagttatac tcatcaagat catatccact gcattcacat
     5821 ctatcagcag cagtgaaggg ttgtatacga aaatgtacgg caccaggaat gccgcaatgg
     5881 ccagttttgt tgcctgtata cccgtcttca gcgggtttgc ctttgcaata ccagccccta
     5941 caaaggccgc tagcgctaca ggcggtgtca cgtctgcaat gatgccgaaa tagaagacaa
     6001 acatatgggc tgctaaaaca gggacatcca tcatataagg gccggagccg ctatggttga
     6061 cgtgattacg tagtttgctg ttgtaggcac gcccattccc aaaataattg aagttagcat
     6121 agtaaataca agtgtgagga aaagtttgcc atcagcaata tcgaccaaaa ccgagccgag
     6181 ccttagaccc agacctgtaa gtgt
//
LOCUS       DAONUO010000097         6193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9488_length_6193_cov_4.045292, whole genome shotgun sequence.
ACCESSION   DAONUO010000097 DAONUO010000000
VERSION     DAONUO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9488_length_6193_cov_4.045292"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="fliP"
                     /locus_tag="PK768_04725"
     CDS             complement(<1..521)
                     /gene="fliP"
                     /locus_tag="PK768_04725"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HQA60305.1"
                     /translation="MLTSFTRTIIVLSFVRNGMGIQQVPPTQVLIGLALFLTIFIMAP
                     VWSQINEQALQPLIYQEITTSEALQRAQIPLRDFMFRQTREKDLALFVHYARLERTIN
                     SLDDIPTYVLIPAFIISELKTAFQMGFVIFIPFLVIDMIVSSTLMSMGMLMLPPIMIS
                     LPFKILLFVMVDG"
     gene            complement(728..1261)
                     /locus_tag="PK768_04730"
     CDS             complement(728..1261)
                     /locus_tag="PK768_04730"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HQA60306.1"
                     /translation="MKRNILLMIILFILISILTAGTLVLANPIDVDNLKNYDFDVIDD
                     NTNHTTSAPLSFLTFVVYFIFFVLIAGLAYLTTRWIGKQQKKIRAKSKYMELIDSLQL
                     SGDNALHIVKSPDGVLLLGTGKEGIFLLGKLDDEEAELIIQAEKNQIEENFSAQLNNY
                     LSKIKWGTDHNKFGESK"
     gene            complement(1276..1638)
                     /locus_tag="PK768_04735"
     CDS             complement(1276..1638)
                     /locus_tag="PK768_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQA60307.1"
                     /translation="MAGILIVDDAAFMRMMIKDILTKNGYQVVGEAENGVAAIKSYNE
                     LNPDLVIMDITMPEMDGIEAVRRIKASDPSARIIMCSAMGQQAMVIDAIQAGARDFVV
                     KPFQPERVIEAVKKALAS"
     gene            complement(1657..2796)
                     /gene="fliY"
                     /locus_tag="PK768_04740"
     CDS             complement(1657..2796)
                     /gene="fliY"
                     /locus_tag="PK768_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HQA60308.1"
                     /translation="MDEQFLSSEELRELTKDFSDPEKSQISSEEIDTLGEIGNISIGT
                     SATTLYSLLRNRVTITTPRVFITNIQELKENHPIPFISVEVSYTKGLSGTNIMIIKER
                     DAKVIADLMMGGDGTNTDVELDDMRMSAVGEAMNQMMGSAATSLSTLLKKDISISPPR
                     LTKINLATDSLKGYFNEHEEIVNISFRMEVGDFLKSEIMQLMPIPFAKGLVQDLHTLS
                     TSSYESESDQDKIESELESARPEKTEVQKPVQITQAQKDMSIKEPKTVRDTGTETRVT
                     VKPVQFQSFEEEQVPAASASIDLILDVPLEITVELGRTNKTIKEILEISPGTIIELDK
                     MSGEPMDILVNGKLVAKGEVVVIDENFGVRITEIIDSIERVKSLQ"
     gene            complement(2789..3784)
                     /gene="fliM"
                     /locus_tag="PK768_04745"
     CDS             complement(2789..3784)
                     /gene="fliM"
                     /locus_tag="PK768_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HQA60309.1"
                     /translation="MSEILSQSEIDALLAALSTGEVKAEDIKSKESDKKIKPYDFKRP
                     DKFSKEQLHTLSMIHENFARLLTTYLSAQLRTLVQINVFFVEQMTYNEFIISVSNPSL
                     IAVIDFSPLKGAALIEINPTIAFAIIDRLLGGTGDYKEKVREPTEIENSIIGKVFNKM
                     TKILTEAWQDVAEIDMTMEKLETNSQFVQLVSPNEAVALITFSAKVGNTEGMLNICIP
                     HIVLEPIISKLSTRIWLSTSKREHSEFTKDVIKEKISDTKSEIIAELGRTSITVRDFI
                     NLTVGDVITLDKSIHDGIDIYVENKLKYSGTVGIYHNKLAVKVQKMYGMEDSFNG"
     gene            complement(3805..4257)
                     /locus_tag="PK768_04750"
     CDS             complement(3805..4257)
                     /locus_tag="PK768_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HQA60310.1"
                     /translation="MATQKSPEKKSGLRNILLVVLALFFLMLMTTGVAYVVAKNIASS
                     NEKVVENPKNHITYDAGEFLTNLADKGYVKLSMVYLLNGKDVEKELEIKDSEVRDKVF
                     TILRSKNHDSVRDSNGMEELRKEIKECLNQLLNSGEIVDVFFTSIIVN"
     gene            complement(4271..5026)
                     /locus_tag="PK768_04755"
     CDS             complement(4271..5026)
                     /locus_tag="PK768_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQA60311.1"
                     /translation="MKTLAGSRRRSNKNNEQALGGSPAWMTTYGDLITQILIFFVLLF
                     SFSNVDRDKFTAAMLSLQGSLGIIDGGMTLQEGDLMEQGEIGEIMISVQEQREFELLH
                     ERVEEIISEDDLNGIQVNLDERGLVIRFVEGVLFDSGKADIKNEARAILDKIAPLLID
                     THRHIRIEGHTDNLPINTREFPSNWELSTARAVNVVKYFIEKHNFSPYILSAAGYGEY
                     RPIAPNDSDKNRALNRRVDIIILKSTSESTEPR"
     gene            complement(4989..5819)
                     /locus_tag="PK768_04760"
     CDS             complement(4989..5819)
                     /locus_tag="PK768_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HQA60312.1"
                     /translation="MQRRIDYSTLIGVFSGVLLIMFAIYNGGTIRTFLNFNSFLITFG
                     GTVAATVANYPLSKLAGLVRVLIVAFTEKPLEPNNVIRTIISLAETARREGLLALEDA
                     AYQLDDEFMQKGILLIVDGADPELVRNIMETELAFLEERHGEGQGIFETMGTFSPAFG
                     LLGTVIGLINMLTQLDNPNAIGAGMAVALVTTFYGSFLANVIFLPLAGKLKVRSREEI
                     LIKEVMIEGMLSIQAGENPRIIEEKLKAFLAPAIRSDVSSYKNVESGEDLGWQQAAQQ
                     "
     gene            complement(5804..6034)
                     /locus_tag="PK768_04765"
     CDS             complement(5804..6034)
                     /locus_tag="PK768_04765"
                     /inference="COORDINATES: protein motif:HMM:NF018043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HQA60313.1"
                     /translation="MITLTKLNGKSFVINADLIETLESTPDTVVTLTTGKKYIVMEKT
                     DDIIDKVIQYRKEIFCSGLQILKGSAAKCNAE"
     gene            complement(6094..6186)
                     /locus_tag="PK768_04770"
     CDS             complement(6094..6186)
                     /locus_tag="PK768_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HQA60314.1"
                     /translation="MIITQRGFQANSRIITASDEMLQELINIKR"
BASE COUNT         1823 a         1285 c         1002 g         2083 t
ORIGIN      
        1 catccgtcaa ccatgacgaa aagcaaaatc ttaaaaggca aagatatcat tatcggaggt
       61 agcatcaaca tacccattga cataagagta cttgatacaa tcatgtctat aactaaaaaa
      121 ggtataaaga ttacaaaacc catttgaaat gctgtcttta attcacttat tataaacgca
      181 ggaatcaaca cgtaagtagg tatgtcgtcg agagaattaa tggttctttc cagcctggca
      241 tagtgaacaa aaagggctaa atctttttct cttgtctgtc tgaacataaa atctcgtaaa
      301 ggtatctgag ccctttgcag tgcctcgctt gtagtgattt cctgatatat aagtggttgt
      361 aaagcctgtt catttatctg actccacacc ggagccatta taaatattgt cagaaacaaa
      421 gcaaggccaa ttagaacctg agtaggcgga acttgctgaa ttcccatgcc atttctaacg
      481 aatgaaagca cgatgatagt tctggtaaaa gatgtaagca ttattaagat tgaaggtgct
      541 aaagatagta tggttagcag cagtattact tgtaaagata aactcaggtc ctctggacta
      601 tcggctggct ctataaatgg aagtgagggt agttgaatac cttcttcagc agcataagct
      661 gtaacatgta aacataaaaa acataaaatt aagcaaatta tcttgggtag aaaactttta
      721 cgcaatatca ttttgattca ccaaatttat tatgatctgt gccccattta attttactaa
      781 gatagttgtt caattgagct gaaaaatttt cttcaatctg atttttctca gcttgaataa
      841 tcagttccgc ttcttcatcg tccagcttgc ctaacaaaaa tataccttct ttacccgtgc
      901 caagcaacaa aacaccatca ggtgatttca caatgtgaag agcattgtct ccactgagct
      961 gcaaactatc aatcagctcc atgtatttac tttttgctct tattttcttt tgctgttttc
     1021 cgatccagcg tgtagtcaaa taggccaatc cggctataag aacgaaaaaa ataaaatata
     1081 caacaaaagt caggaagctc aaaggggctg aagttgtgtg atttgtgttg tcatcaatga
     1141 catcaaaatc ataatttttc aagttgtcta catctatcgg atttgccaaa acaagggttc
     1201 cggcagttaa gattgaaata agtataaaca atataatcat caatagaatg tttcttttca
     1261 tttagtaaaa cctctttaag acgcaagagc tttttttacc gcctctataa ccctttcagg
     1321 ttgaaaaggc tttactacaa aatctctggc accagcctga atagcatcaa taaccatcgc
     1381 ctgttgtccc atagcagaac acatgataat gcgcgcagaa ggatcactag cttttattct
     1441 tctcaccgct tcaattccat ccatctcggg cattgttata tccataatta ctaaatcagg
     1501 atttaattca ttataacttt ttattgctgc aactccattt tctgcttcac ctactacttg
     1561 gtatccgttt ttagtaagta tatcttttat catcatcctc ataaaagcgg catcatctac
     1621 tattaatatt cctgccattt gactcatcct cctctattac tgtaaacttt taactctttc
     1681 aatagagtct attatttcag ttattctcac gccaaagttt tcatcaataa caacaacttc
     1741 acccttggct accaacttgc cattaactaa aatatccatg ggttctcccg acattttatc
     1801 cagctcaata atggttccgg gagatatttc taggatttct tttatcgtct tgtttgtcct
     1861 acccagttct acggttattt caagaggaac atctaatatc aaatctatgc tcgcactagc
     1921 agctggcact tgttcctctt caaaactttg gaactgaact ggcttgaccg taaccctagt
     1981 ttcagttccg gtgtccctaa ctgttttagg ttcctttatt gacatatctt tttgtgcctg
     2041 agtaatttga acaggttttt gcacctctgt tttttcaggc cttgcacttt ccaactcgga
     2101 ttcgattttg tcttgatccg attctgattc gtatgaagaa gtagacaagg tatgaagatc
     2161 ttgaaccaaa cctttagcaa aaggaatagg cattaattgc ataatttcgc tttttaaaaa
     2221 atcacctact tccattctaa aagatatatt tacaatttct tcatgttcat taaaataccc
     2281 ttttagcgaa tcggtggcca ggttgatttt agtcaagcgt ggaggtgata tgctaatatc
     2341 tttttttaac aaggttgata aggaggtagc cgcagaaccc atcatttgat tcatggcttc
     2401 tcctacagcg ctcattctca tatcatccaa ttccacatca gtgtttgtac catcacctcc
     2461 catcattaaa tccgcaataa cctttgcatc tctttccttg atgatcataa tattggtacc
     2521 gctcaagcct tttgtataag atacttcaac gctaataaag ggtatgggat gattctcttt
     2581 taattcttga atatttgtaa taaatactct aggtgttgtg atggtaactc tatttctaag
     2641 caaagaatac aaggtagtag cagatgtccc aatagaaata ttgccgattt cacctaaagt
     2701 gtcgatttcc tcacttgaaa tttgactttt ttcaggatcg gaaaaatctt ttgtaagttc
     2761 tcttaactct tcagaagata ggaattgctc atccattgaa tgagtcctcc attccataca
     2821 ttttttggac ttttacagcc aatttattgt gatagatgcc tacggtgcct gaatatttta
     2881 atttattttc aacatatatg tcaattccgt catgtatact tttgtctaag gttataacat
     2941 cgcccacagt cagatttata aagtctctaa cggtgataga agttctgcca agttcagcta
     3001 taatttcaga cttcgtatca gatatttttt cttttattac atcttttgtg aattcagagt
     3061 gctctctttt gcttgttgat aaccatattc gtgtagacaa tttagatata attggttcca
     3121 atacaatatg gggaatacat atatttaaca tgccttccgt atttcccact tttgcactaa
     3181 aagtaatcaa agcaactgct tcattgggcg ataccagctg gacaaactga gagtttgtct
     3241 caagcttttc catagtcata tcaatctctg ctacatcttg ccatgcttca gttagtatct
     3301 ttgtcatttt gttaaagact ttaccgatta tgctgttttc aatttctgta ggctcccgca
     3361 ccttttcttt ataatctccg gttccgccca acaatctatc aataattgca aaagcaatag
     3421 ttgggtttat ctcaattaga gctgcaccct tcaaaggaga aaagtctata accgctatta
     3481 aagaaggatt tgaaaccgat ataataaatt cattgtaagt catttgctca acaaaaaaaa
     3541 cgttaatctg gacaagagtt cttaactgag ctgaaagata agtggttaac aagcgagcaa
     3601 aattctcatg aatcatactt aatgtatgaa gctgttcctt tgaaaacttg tcagggcgct
     3661 taaaatcata tggtttgatt tttttatccg attctttgct tttaatgtcc tcagctttca
     3721 cttctccggt ggataaggcg gcaaggagtg catcgatttc actttgagaa agtatttcag
     3781 acaattgtgt tcgcccccat ctacttaatt tacaatgatg ctagtaaaga aaacatcaac
     3841 aatttctccg ctatttaaca attgatttaa acactctttt atttcctttc taagctcttc
     3901 cataccattg ctatctctaa ccgagtcatg atttttggac cgtaatattg taaatacctt
     3961 gtcacgaact tcactgtctt tgatttctaa ttccttttca acatctttac cattcagtaa
     4021 atagaccatg gacagtttta cgtatccttt atctgctaaa tttgttagaa actctcctgc
     4081 atcatatgta atatggtttt tgggattctc tacaactttc tcattgcttg aagcaatatt
     4141 ttttgctact acatacgcta caccggtagt cattagcatc aagaaaaata atgccaacac
     4201 tactaacaaa atatttctca agccactctt tttttctggg cttttctgcg ttgccaatgc
     4261 ccatcctcct ttatctaggc tcagtggatt ctgatgttga ttttaatata ataatatcca
     4321 cacgcctgtt taaagctctg tttttatctg aatcatttgg agctataggt ctgtattcac
     4381 cataacccgc agcagataaa atgtatggag aaaaattatg tttttctata aagtacttta
     4441 caacgttaac agctcttgca gtggacaact cccaatttga tgggaattcg cgtgtattaa
     4501 tcggtaaatt gtcagtgtgg ccttcaatcc ttatatgtct gtgagtgtca atgagcagtg
     4561 gggcaatttt gtctaaaatg gctcttgctt cattttttat gtcagcttta ccggaatcaa
     4621 ataaaacccc ttcaacaaat ctgatgacga gacctcgctc atctagattt acttgtatac
     4681 cattcagatc gtcttcagaa atgatctcct cgactctttc atgaagtaat tcgaattccc
     4741 tctgctcctg gacagagatc atgatttcac caatctctcc ttgctccatt aaatcgcctt
     4801 cttgtaaagt cataccacca tcgattattc ctaaggaccc ttgcagcgaa agcatggctg
     4861 cagtaaattt gtctctgtcc acattagaaa aggaaaagag caaaacgaaa aatatgagaa
     4921 tctgcgttat taaatctcca tatgtagtca tccatgcagg agaaccacct aatgcttgct
     4981 cattattttt attgctgcgc cgcctgctgc cagccaaggt cttcaccact ttcaacattc
     5041 ttatatgaac ttacatcact tctgatagca ggagcaagga atgcctttaa tttttcttct
     5101 atgatcctcg ggttttcacc agcttgaatt gataacatgc cttctatcat cacttctttg
     5161 atgagtattt cctctctgct tctaacttta agtttgcctg ctagtggtaa aaaaatcacg
     5221 tttgccagaa aagaaccata gaaagtagta acaagagcaa ctgccatacc ggcccctatg
     5281 gcattaggat tgtccagttg agttaacata ttaattagtc ctattacagt gccaagcaaa
     5341 ccaaaggcag gggaaaaagt gcccatggtt tcaaagatgc cctgcccttc accatgtctc
     5401 tcctctaaaa aggcaagttc agtttccata atatttctta ccagttctgg atccgctcca
     5461 tcaactatta gcaagatgcc cttttgcata aactcatcgt ctaattgata agcagcatct
     5521 tcaagggcaa gaaggccctc cctccgagca gtttcagcaa ggctaataat ggttctaatt
     5581 acattgtttg gttccaaagg cttttcagta aaagcgacta ttaagaccct aactaaaccg
     5641 gccaatttag ataaggggta gttggctact gtagctgcta cagttccgcc gaaagttatt
     5701 aaaaaggagt tgaaatttaa aaatgtcctt atggttcccc cgttataaat tgcaaacatt
     5761 atcagtagaa cgccgctaaa gactccaatt aaggttgaat aatctattct gcgttgcact
     5821 tagctgcact tcccttcaag atttgaagtc cactacaaaa gatttctttt ctgtattgta
     5881 ttaccttgtc aatgatatca tcggttttct ccattactat atatttcttt cctgtagtca
     5941 atgtcaccac ggtatcagga gttgattcca aagtttcaat caaatcagca ttgattacaa
     6001 agctttttcc gttaagcttt gtgagtgtta tcatatttaa caacctttca attttcccgg
     6061 gcttgacaaa atcaagcccg ggaaactagt attttatcgc tttatgttaa ttaattcttg
     6121 aagcatttca tcagaggcag taataatcct ggaatttgcc tgaaagcctc tctgggttat
     6181 tatcatctgt gta
//
LOCUS       DAONUO010000098         6184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9505_length_6184_cov_3.042748, whole genome shotgun sequence.
ACCESSION   DAONUO010000098 DAONUO010000000
VERSION     DAONUO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9505_length_6184_cov_3.042748"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="spoIIIAA"
                     /locus_tag="PK768_04775"
     CDS             complement(<1..798)
                     /gene="spoIIIAA"
                     /locus_tag="PK768_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HQA60315.1"
                     /translation="MGIKGNKSIEIVLQNEILPILPLHIAKTIESIDIKYLLSLEEIR
                     LRAMKPLMLTINNEAFMLTNNGEITRNCDLAYYVTKEDINKSYHFISQCSVYSFEEEL
                     KNGYITISGGHRVGFSGHVILENNDIKAIRNISSLNIRIAREIIGAADKVLPYIVDKG
                     RLLNALIISPPKAGKTTLLRDLIRQLSNGVDRLGINGINVGLVDERSEIACCYEGVPQ
                     NDVGMRTDVLDGCPKAKGIMMLLRSMSPDLIATDEIGKNEDALAIEEV"
     gene            complement(919..1350)
                     /locus_tag="PK768_04780"
     CDS             complement(919..1350)
                     /locus_tag="PK768_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60316.1"
                     /translation="MYDISLKVSYVKGLVEGLEIEDEKVKRVLSEIISILDDMADAIN
                     DLKMTIDDTQEYVECIDEDLGELEDDFYDMDYEDDDYDEYDDFDDYDDDEFDFDDDDF
                     VEADCPNCRETVYIDKDFLDDGKAECPNCKATIVFDDAENE"
     gene            complement(1373..1930)
                     /gene="efp"
                     /locus_tag="PK768_04785"
     CDS             complement(1373..1930)
                     /gene="efp"
                     /locus_tag="PK768_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQA60317.1"
                     /translation="MISTNDLKTGVTVEIDGEVFMIVDFQHVKPGKGAAFVRTKIKNV
                     KTGQVFERTFRAGEKLNRAIVERKTMQYIYSEGDVYYFMDTQTYEQIPLNKDQLGDSV
                     DYLKENLEVMVMLHEGVSIGIELPTFVELEVTETEPGFKGDTATGGSKPATLETGLTV
                     QVPLFINIGDIIRVDTRTGEYLGRV"
     gene            complement(1946..3019)
                     /locus_tag="PK768_04790"
     CDS             complement(1946..3019)
                     /locus_tag="PK768_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQA60318.1"
                     /translation="MIQARIKKLREKLLNHSIKGLIVSKPENIYYLSAFSGEGLTIIT
                     DEVNYIITDFRYVEQAKHETTGFEIVETRPGVSIFSAAYELIKELNLKNIGVESHSLT
                     VKEYDELSDICEGVNLIKTEGLVEDLRIIKETSEIAFIKSAQQITDKAFEHILAFIKP
                     GVRELDLVAELEYFMKKSGSKNTAFETILISGPKTSLPHGVPSERKLQRKDFVTIDFG
                     ARFKGYCSDMTRTVIIGEPSEQQLSIYNAVLDAQNKALEAIKPGLKGKDIDDVARRHI
                     SDRGYGNYFGHGLGHGVGLEIHEAPRLSPNVENVLLPGMVVTVEPGIYIKHFGGVRIE
                     DMVVLTEKGCENLTHSEKALICL"
     gene            complement(3134..4213)
                     /locus_tag="PK768_04795"
     CDS             complement(3134..4213)
                     /locus_tag="PK768_04795"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HQA60319.1"
                     /translation="MKRIGILTGGGDAPGLNAVIRAVARVAEAAGVELYGFKNGFSGL
                     IKGEFIVLTKPYISGLLPKGGTILGSNNRDNPFNFPVKEGEKLIYKDMSDTALQNLKL
                     FGIECLVVIGGDGTLAIAKELSQKGARIVGVPKTIDNDLNGTEQTFGFDTAVSVAVDA
                     LDRLHTTAESHHRVMVLEVMGRYAGWIALYSGVAGGADVILIPEIPWSIEKVAEKIYK
                     RREEDKYFSIVVVAEGVKTHDGSQVLKKVIDGSSDLIRLGGIAYVIADMVEKVTGIES
                     RATVLGHLQRGGSPSHFDRILATRYGVEAAKLAISGKSGCMVSLQNGKITFIDFEDIP
                     KLIRAVPRDHQLIKAARELGISFGD"
     gene            complement(4237..4815)
                     /gene="pilO"
                     /locus_tag="PK768_04800"
     CDS             complement(4237..4815)
                     /gene="pilO"
                     /locus_tag="PK768_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HQA60320.1"
                     /translation="MIKYPVFLKKNSTKREKGLIFLIVMCLVLYAIHSLLAPKIGKLN
                     SLKSQLIAVNNERASYEQSYNKYHDYDSIMAEYDNLKKKVPIDNDLSKFIRDIENWCQ
                     HSDITIISIVPQETKTEQIEEIEVNLIPCEITIRGNFDLLLTFVDKLENYHRICRINE
                     IKLTGLSERNSSPSLDWKLTINVGLYYIPPTT"
     gene            complement(4812..5372)
                     /locus_tag="PK768_04805"
     CDS             complement(4812..5372)
                     /locus_tag="PK768_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQA60321.1"
                     /translation="MHKLNLLPKEILISQKKKKLGVFCIIAGSLLLILFIIFFTKVDN
                     SIKLYENKIYVANKDISEMRSKLNYYFGQKTLRDFEKRQNFYNAAARDKTNYSAILSD
                     IVNLLPNDINVSSISVNKSNIIKINGFTPGHKHVALLIDDLKTLDGVQEVTLGFTRLI
                     STSDEEGSIGVYDFEISILLVKDRWQ"
     gene            complement(5376..>6184)
                     /locus_tag="PK768_04810"
     CDS             complement(5376..>6184)
                     /locus_tag="PK768_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HQA60322.1"
                     /translation="IYNKSIIKRLYQIKNPIGKVVFENQQEQDTIQQLFSTIKQKFPS
                     RGVIMGIPSNHAVLRYVNFPILSGKELKEAIFWEMQEFNTIIRDEYVSDYEILDEKNN
                     ICRVLLVAVPKYLAMTYANILLGAGFYIKALDLYPLAHARVLKTKNKSGVSAIIDFNY
                     IHNEITIVENGSLILNRNLDFHHDSTIEQKVIEISKIFNFYSLQSKSKQIEEIILLGK
                     AREQREIFKSSFNINVYTDNEIELDFIIDNLQVMDKPIGFFSAIGFALRG"
BASE COUNT         1746 a         1344 c          973 g         2121 t
ORIGIN      
        1 gacttcttcg attgccagag catcttcatt ttttcctatt tcgtcggttg cgattaaatc
       61 cggtgacata gagcgaagaa gcatcattat gcccttggcc tttgggcagc cgtccagtac
      121 atccgttctc atgccaacat cgttttgagg gactccctca tagcagcaag caatttccga
      181 ccgctcatca accagtccga cattaattcc attgattcca agtctatcta ctccgttgct
      241 gagctgtctt atcaggtccc taagtagagt tgttttgcct gcttttggag gagatataat
      301 taatgcattt aacagccgac ccttatctac tatgtagggt aagactttat ctgccgctcc
      361 gattatctcc cttgctattc ttatgtttaa gctcgatatg tttctgatag ccttaatatc
      421 attattctct aaaatcacat gacctgaaaa accaactcta tgaccacccg aaatggttat
      481 atagccattt ttcaattctt cttcaaagga atacactgag cattgactta taaagtgata
      541 ggatttattg atatcttcct tcgttacgta ataagctaaa tcacaattcc gggttatctc
      601 cccattattt gtgagcataa aagcctcatt gtttattgtt agcattagcg gtttcatagc
      661 tctaagcctg atctcttcta aactcaaaag atattttatg tctatggatt ctatggtttt
      721 tgctatgtgc aaaggcagta tgggcaatat ttcattttgt aatacaattt caattgattt
      781 attaccttta atgcccataa ttgtcttgtc ctcctcttta taaatttata tttaatgctt
      841 tttatgttta tgactgaaat aaaaaaaaga gggctaaaac cctcaagttt aaaaccctct
      901 ttaattttgc taataatatt attcattttc tgcgtcatca aatacaattg tcgctttgca
      961 atttgggcat tctgccttgc catcatctaa aaaatctttg tcaatatata ctgtctcacg
     1021 gcaatttgga caatctgctt ctacaaaatc atcatcatca aaatcaaact catcatcgtc
     1081 gtaatcatca aagtcgtcgt attcatcata atcatcatct tcataatcca tgtcgtaaaa
     1141 atcatcttcc agttctccca aatcttcatc tatgcattct acgtattctt gtgtgtcatc
     1201 tattgtcatt ttaagatcat ttattgcatc agccatgtca tccaaaatac taattatctc
     1261 agatagtact cttttgactt tttcatcttc tatttccagg ccttctacaa gacctttaac
     1321 ataagatacc tttaagctaa tgtcatacat ctcttgatcc tcctttttta tattatactc
     1381 tgcctaaata ttcccctgtt ctcgtatcta ctcttattat atcgccgata ttgataaaca
     1441 gcggcacctg gactgttaga ccggtctcca gggtggctgg tttggaacct cctgttgcag
     1501 tatcgccttt aaatccaggc tcagtttcag ttacctcaag ttctacaaat gttggtaatt
     1561 cgattcctat ggaaacgccc tcatgcaaca taaccataac ttccaagttt tcctttagat
     1621 aatcaaccga atctcctaat tggtctttat tgagaggaat ctgttcataa gtttgggtat
     1681 ccataaaata gtatacatca ccttcagaat atatatactg catagtcttt ctttccacaa
     1741 tagcacgatt cagtttttcc cctgctctaa aggttctttc aaagacttgt ccggttttta
     1801 cgttctttat tttcgttctg acaaaagcag caccttttcc tggttttaca tgctgaaaat
     1861 ctacaatcat aaaaacttcg ccatcaattt caacggttac acctgttttt aagtcgttag
     1921 tagatatcaa gaaaaacacc tccagttata aacagattaa agctttttcg ctatgagtta
     1981 aattctcaca tcccttttca gttaatacga ccatatcctc tatccttacc ccaccgaaat
     2041 gttttatgta aattcccggc tccactgtca ctaccattcc tggtaaaagt acgttttcaa
     2101 catttgggga cagtcttggg gcttcatgga tttccaatcc tacaccatga cctaaaccat
     2161 gaccgaaata attgccataa cctcgatcgg aaatgtgtct tcttgccaca tcatcaatat
     2221 ccttaccttt taagccgggt ttaattgctt ccaaagcctt gttttgggca tcaagaaccg
     2281 cattgtagat tgacagttgc tgctctgatg gctcccctat tattacggtc ctggtcatat
     2341 ccgaacaata gcctttaaag cgagcaccaa aatcaatagt aacgaaatct ttacgctgca
     2401 atttgcgttc agatgggaca ccatggggca acgaggtttt aggaccggaa attagtatgg
     2461 tctcaaaggc tgtattttta gagccgcttt ttttcataaa gtattctagc tcggctacta
     2521 aatccagttc cctcactccg ggtttaataa aggctaatat atgctcaaaa gctttgtcgg
     2581 ttatttgctg tgcagatttt atgaaggcga tttcactggt ttctttaatg attctcagat
     2641 cttcaactag accctcagtt tttattaaat taacaccttc acaaatatca gaaagctcat
     2701 catactcttt aacggtcaat gaatggcttt caacaccgat attcttcaag ttcagctctt
     2761 ttataagctc atatgcagct gaaaaaatgg aaacaccagg tcgggtctct acaatctcaa
     2821 agcctgttgt ttcatgcttt gcctgctcaa catatctaaa atcagtaata atatagttta
     2881 cttcatcggt tattatcgtt aaaccttcac cagaaaaagc acttagataa taaatatttt
     2941 ccggtttgct aacaattaat ccctttatac tatgatttaa tagtttttcc cttaattttt
     3001 ttatacgagc ctgtatcaag caatcccccc tttaaaagga taacattact atcgtgagta
     3061 tttcccccag agtgcataaa tattctttta taatattatc atatttcctt ttttttgaat
     3121 aggcccttat caactaatct ccaaagctga ttccaagctc ccgggcagcc ttgattagct
     3181 gatgatccct cggtaccgcc cttatcaatt ttggaatatc ttcgaaatcg ataaatgtga
     3241 ttttaccatt ttgcagggat accatgcaac cggatttgcc agatatagcc aattttgccg
     3301 cctcaactcc gtatcgggtt gcaagtatcc tgtcaaagtg agaaggtgat cctccacgct
     3361 gcaagtgacc tagaacggtt gcccggcttt cgataccggt aactttttcg accatatctg
     3421 ctatgacgta agcgatgcct ccgagtctaa tcagatcgga gctaccatcg atcacctttt
     3481 ttagcacttg gctgccatca tgagtcttaa ccccttcagc tacgaccaca atgctgaaat
     3541 acttgtcttc ttccctgcgc ttatatatct tttcggcaac tttttcaatg ctccaaggga
     3601 tttccggtat gagaatgaca tcggcaccgc ctgcaacacc gctgtatagg gctatccaac
     3661 cggcatatct gcccatcact tcaagcacca ttaccctatg atgagattct gctgttgtat
     3721 gcagtcgatc taatgcatct acagctactg aaactgctgt atcaaagcca aaagtctgct
     3781 ccgttccgtt aaggtcattg tcgatggttt tgggaacacc aacaatcctt gcgcctttct
     3841 gagatagttc ttttgctatg gcaagcgtcc catctccgcc tattactacg aggcactcga
     3901 tcccgaacaa tttcagattt tgaagggctg tatccgacat atctttatat attagctttt
     3961 cgccttcctt caccgggaaa ttaaatgggt tgtcccggtt gttggaaccg agaatagtac
     4021 cgccctttgg caggaggccg gaaatatatg gctttgtcag cactatgaat tcccctttta
     4081 ttaggccgct gaaaccattc ttaaaaccgt aaagctccac acctgcagcc tcagccaccc
     4141 tggccacggc cctaataaca gcattgaggc caggagcatc tccgcctccc gtaagtatgc
     4201 caatcctttt caccatcaac acctcttatt ctatgtttaa gttgttggag gaatataata
     4261 gagaccgaca ttaatggtaa gtttccagtc taaagaaggg gaggaatttc tttctgaaag
     4321 tcccgttaac tttatctcgt taattcgaca tattcgatgg taattttcca acttgtcaac
     4381 aaaagtcaag agtaaatcaa aattgcccct tattgttatt tcacagggaa ttaagttgac
     4441 ctcaatttct tcgatttgct ccgtttttgt ttcctgagga acgatcgaaa ttatcgtgat
     4501 gtcactgtgc tgacaccagt tttcaatatc tcttatgaac ttcgataaat cattgtctat
     4561 gggcactttt ttcttcaaat tatcatactc agccattata ctatcgtaat catggtactt
     4621 attatatgat tgttcatatg aggccctctc attgttgaca gcaatcaatt ggctttttaa
     4681 gctgttcagt ttgccgattt tgggtgctaa aagtgaatgt atagcgtaaa ggactaaaca
     4741 catgacaatt aaaaagatta atcctttttc tctcttcgtg ctgtttttct tcaaaaaaac
     4801 agggtactta atcattgcca cctgtccttt accagaagga tagatatttc aaaatcataa
     4861 accccaattg acccttcctc atcgcttgta ctgatcaatc gggtaaaacc aagcgtcact
     4921 tcttgaacac catctaaagt ttttagatcg tcaatgagca gtgctacatg tttatggccg
     4981 ggagtgaaac cattaatttt tattatatta gatttattaa cactgattga agaaacgtta
     5041 atatcatttg gcagcagatt gacaatatcg ctaaggattg cagaataatt agttttatct
     5101 ctcgccgcag cattataaaa gttctgcctt ttttcaaaat ctcgcaatgt tttttggcca
     5161 aaataataat ttaatttaga tctcatttca gatatgtctt tattagcaac atatatttta
     5221 ttctcataaa gctttattga attatcgact ttcgtaaaaa atatgataaa taaaatcaat
     5281 aaaaggcttc ccgctataat acagaaaaca cccaactttt tcttcttttg actgattagt
     5341 atctctttcg gaagaaggtt taatttatgc atagtctaac ctctcaaagc aaaacctata
     5401 gcgctaaaaa aaccaattgg tttgtccata acttgtaaat tatctataat aaaatccaat
     5461 tcgatttcat tgtcagtata gacattaatg ttaaaagaac ttttaaatat ttccctttgt
     5521 tctcttgcct ttccaagcaa aataatttcc tcaatttgct tgctcttact ttgcagagag
     5581 taaaaattga aaattttaga aatttctatg accttttgtt caatggtact gtcatggtga
     5641 aaatcgagat ttctatttaa aattaagctg ccgttttcca caatagtaat ctcattatgt
     5701 atataattaa agtctattat ggcggatacg ccagatttgt ttttagtttt taacacccgt
     5761 gcatgagcga gaggatatag gtctaatgct tttatgtaaa atcccgcccc aagcagtata
     5821 ttggcataag tcattgctag gtatttagga accgccacca ataaaacacg gcagatattg
     5881 tttttttcgt ccaatatttc ataatcactt acgtactcat cacgaataat cgtattaaac
     5941 tcctgcattt cccaaaatat tgcttctttg agctctttgc cactgagtat aggaaaattg
     6001 acatacctta atactgcatg gttacttgga atccccataa ttacaccgcg agaaggaaac
     6061 ttttgtttaa ttgttgaaaa aagctgttgg atggtatcct gttcttgttg attttcaaag
     6121 actacttttc caatcgggtt ttttatttga taaagtcttt ttattatgct tttattatat
     6181 atta
//
LOCUS       DAONUO010000099         6090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9675_length_6090_cov_3.504391, whole genome shotgun sequence.
ACCESSION   DAONUO010000099 DAONUO010000000
VERSION     DAONUO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9675_length_6090_cov_3.504391"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /locus_tag="PK768_04815"
     CDS             complement(<1..1713)
                     /locus_tag="PK768_04815"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HQA60323.1"
                     /translation="MVFFVMRRKSLLAMLVTMVLLFGLLFPGQVTFAADSTTSHFKGG
                     NGSENAPYLIANAEQLDKVRDYPNAHFKLIDDIDLGVPPYNGGKGWEPIGSEDSPFAG
                     SLDGDGHTIKNLYIDREDEQDIGLFGVLDEGSRVKNLNLENVDINGGDFVGGLVGWNN
                     KGQIKNICVSGIINGEDYVGGLAGGNGGIVTECYADGEVSGNYVIGGLVGENYYGSIT
                     DSYAEADVSGEHYIGGLVGSNWGDVAGCYATGDVDGDDEIGGLVGYNEGEIKDCYATG
                     DVRAKYGAAGLVGWNFYNGEIIRCYATGNVNSDDETGGLVAYNNAGMIFDSYYDSSTT
                     GQSDNEMGQPKITEEMKLRSTYKTWDFVDVWVIEDGESCPILRWQDEAPQWGFGVSVP
                     STVEPVEKFKIEISNAKDEKGYLISGNKKVTVCDTVYGKVYGEDGDGILFQLTIEFDN
                     GKADFPITLNTPGRYELRVRVEDILYSNLVTLDVLSPIFAGGSGTEEDPYQIAEPEHL
                     YNVRYRSEAYFKLIEDIDLNNTPFNKNQGWRPIGSWDKPFTGSFDGNGKTIRNLYINR
                     GDEDY"
     gene            complement(1827..3989)
                     /locus_tag="PK768_04820"
     CDS             complement(1827..3989)
                     /locus_tag="PK768_04820"
                     /inference="COORDINATES: protein motif:HMM:NF036792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60324.1"
                     /translation="MSKFKKALALAIMMVFLLQAGISFAKPSDVEGHWAQEQIMEWLD
                     KGWTQYGDDGKFYPDAKITRGEFIALTNKAFGFEKTTQVNFPDLHEDHMYAEDVAKAV
                     AAGYISGFDDGTVRPDEQISRVEVTVILVKIIAELPLPQDESVLTKFSDYEDIPDWAR
                     TFVAVAVHNELLSGYPDGSFRPQGNITRAEAITVLNNAKSIFSFLSEKITKPGNLEIK
                     PGIVDSGSSGRIVLFYSLGDDFTKGSVIFQLPAGIKAVAGRDWVAISGTGRDTEIIWL
                     DPDNIQNEGREVRVTDVTAAEGSVVMLLLEGITMPEPGFHAFKVIADADGSGEKLPTE
                     NDQYGPTELFSYYETTELFSRIPLGYEGSLELSPQSAKSGSKQTISLTYTFGNDFNEG
                     CVEFSLPEEISFTAGQDKVIINGVEKILAEEDIGDNGQKVHIKGITAQKGDRLTMTIY
                     DKLIPEAGAYFFAVKADVDGEEGSKPATLGSGREFMAFLSYTEATDNYNIVESFFRAI
                     NEGDAEAAIALLADDVVFVDNYLDGFLYMDDGKENVEEIIKSFIKYESRMKYDDSNIR
                     KLSENIWQVEGIVNDYSDIIIAEMYPDEGYEGFRYTSKFFVTDNKICYIEFQFNLEDE
                     LLWDKLTGGPIGFSTYLNDSDEVEVIACVPGMPADKAGILPGDIIVAVDGIDVQDMKF
                     ALEEVAIRVSGKAGTKVKLTINRDGQVFDIEVERAASW"
     gene            complement(4176..4622)
                     /locus_tag="PK768_04825"
     CDS             complement(4176..4622)
                     /locus_tag="PK768_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQA60325.1"
                     /translation="MQKSQEESFYTDVLINELYPDESYKGFRYVSKYFVTDDKICYVE
                     FLWNQDDEMAWHELINGKIGIFGYENDKNEIVIQGCVPGMPADKAGLMPGDIIVAIDE
                     LYISDMKYGEDEAWLRLSGKAGTKVTLTINRNGQVFDVEVERVAGW"
     gene            complement(4576..4800)
                     /locus_tag="PK768_04830"
     CDS             complement(4576..4800)
                     /locus_tag="PK768_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60326.1"
                     /translation="MSKIKKISVALAIIMVFLLQTGISFAQPTDIEGHWAQEKIMAWL
                     EKGWAEIGEDGRFYPDAKITRGEFLYRCID"
     gene            complement(5001..5249)
                     /locus_tag="PK768_04835"
     CDS             complement(5001..5249)
                     /locus_tag="PK768_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60327.1"
                     /translation="MVLAERALRMLKKLETHGGDSDLDRFEDKSLIAEYAIDSIASLV
                     KEGLIVGSENRINPLDNTSRAEAAVFLYRIYTMIYGSK"
     gene            complement(5334..>6090)
                     /locus_tag="PK768_04840"
     CDS             complement(5334..>6090)
                     /locus_tag="PK768_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60328.1"
                     /translation="AIDEIVPNEQGIKLLEIDMTKSEGAKAYEMILPPGALTAGDANI
                     NINTAIGSITLPGNMLPEELTGAKTVSMSIALADKSKLSQELQQRIGDRPVIELNLKV
                     DGKSYSWSNDDVPVTVAIPYTPTEEELRSPEHITVWYIDSKGNIIEVPSGRYDPETSS
                     VIFSITHFSQFAVVYVTKAFDDLDTVPWARKAIEVLASKGIIRGKTETEYAPQTDISR
                     AEFLYSLVRTLGVTASFDENFDDIEIGAYYYKE"
BASE COUNT         1583 a         1536 c         1039 g         1932 t
ORIGIN      
        1 ataatcctca tcgccccgat ttatgtaaag atttctgata gtttttccat tgccgtcaaa
       61 ggagccagta aaaggcttgt cccagctacc aattggcctc caaccctggt ttttgttaaa
      121 gggtgtgttg tttaaatcaa tatcctctat caacttaaaa taggcttcag accggtacct
      181 tacattatac aaatgttctg gttcagctat ctggtaagga tcttcttctg tgccggaacc
      241 accggcaaat atgggagaaa gcacgtctaa tgtaactagg ttagagtaca aaatatcttc
      301 aactctaacc cttagctcat atctgccagg tgtatttaaa gtaatcggga aatcagcttt
      361 gccattatca aactcaattg tgagctgaaa tagaattcca tcaccatctt ctccgtatac
      421 cttaccgtat accgtatcgc ataccgtcac ttttttattg ccggaaataa ggtaaccttt
      481 ttcgtctttg gcattagaaa tttctatctt aaatttttcc acaggctcca cagtactagg
      541 aactgatacc ccaaagcccc attgcggtgc ttcatcctgc cagcgaagaa tgggacagct
      601 ctccccatct tcgatgaccc aaacatccac aaaatcccat gtcttatatg ttgagcgcag
      661 tttcatttct tcagtaattt taggctgccc catctcgtta tctgattgac cggtagtgct
      721 gctatcataa taggagtcaa atatcatccc tgcgttgttg tatgccacga gccctccagt
      781 ttcatcatca gagttaacat ttcccgtcgc atagcagcga atgatttccc cattataaaa
      841 gttccatccc actagaccgg cagctccata ttttgctctt acatcgcccg ttgcataaca
      901 gtccttgatt tctccttcat tgtacccaac caggccgccg atttcatcat cgccgtcaac
      961 atctcctgta gcgtagcacc cagctacatc tccccaatta gaaccaacta atccgccgat
     1021 atagtgctcg ccagagacat cagcttcagc gtagctatct gtaatacttc cgtagtagtt
     1081 ctctcctacc aatccaccga taacgtagtt gccggagacc tcaccatctg cataacactc
     1141 tgttactatc ccaccgtttc cacctgctaa accgccaaca taatcttcgc cattgataat
     1201 accagaaacg cagatgtttt taatttgtcc tttgttattc caccccacga gaccgccgac
     1261 aaaatctccc ccgtttatat ccacattctc aaggttgaga ttctttactc ttgagccttc
     1321 atccaaaacg ccaaacaaac ctatatcttg ctcatcttcc cggtctatat atagattctt
     1381 gatggtatgt ccgtcaccat ctaaggagcc ggcaaaaggc gaatcttcgc ttccaatggg
     1441 ctcccagcct ttgccaccgt tatagggtgg cacacctaaa tcaatatcat ctattaactt
     1501 aaagtgtgca ttagggtaat ctctcacctt atccaattgt tctgcatttg ctataaggta
     1561 aggggcattc tccgaaccgt taccaccttt aaaatgcgaa gttgtgctat cagctgcaaa
     1621 ggtgacctgt cctggaaaaa ggaggccaaa aagaagcacc attgttacaa gcatcgccaa
     1681 aagggatttc ctgcgcatta cgaaaaatac caatggtcta tacccaccat atttgttcct
     1741 cttttttatt aaccttcact ttatagcaca gccgaacctt ccgctgtact aagcaaacgg
     1801 aaggtccggc attattattt atgcctttac caactagctg ctctctcaac ttctatatca
     1861 aacacttgcc catcccggtt tatagtcagc tttacctttg tgccagcctt gcctgaaact
     1921 ctaatcgcaa cttcttcaag agcgaatttc atatcctgaa catctatacc atcaacagcc
     1981 actataatat ctcccggcag tatacccgct ttatcggctg gcatcccagg cacacatgca
     2041 atcacttcaa cctcgtcact atcattaaga tatgtggaaa agcctatggg gccacctgtt
     2101 agtttatccc agagtaactc atcttccaga ttgaactgga attcaatgta acaaatctta
     2161 ttatcagtta caaaaaactt actggtgtag cggaaacctt catagccttc atcagggtac
     2221 atttcagcta ttataatatc agaatagtca tttactattc cttctacttg ccatatattt
     2281 tctgatagtt ttctgatatt actatcatcg tatttcatgc ggctttcata cttgatgaaa
     2341 gattttatta tttcttcaac attttccttt ccgtcatcca tatacagaaa gccatccagg
     2401 taattgtcca caaaaaccac gtcgtcagca agtagggcta ttgcagcctc tgcatctccc
     2461 tcatttattg ccctaaaaaa gctctcaact atgttgtaat tatctgtcgc ttctgtatat
     2521 gatagaaacg ccataaattc tctgccgctt cccagggttg caggctttga accttcttca
     2581 ccgtctacat ctgccttaac tgcaaaaaag taggcacctg cttccggaat aagtttatcg
     2641 taaattgtca ttgtcaacct gtcgcctttt tgtgcagtaa tccctttaat gtgcaccttt
     2701 tgaccattat cgccgatgtc ttcctctgcc aatatttttt ctacaccgtt tattattact
     2761 ttatcctggc ctgctgtgaa gcttatttcc tccggcaaac tgaattcaac gcatccttca
     2821 ttaaaatcat tcccaaatgt gtaagtcaat gatatggttt gcttgctgcc cgatttggca
     2881 ctttgtgggc ttagttccag gctgccttca tatccaagag gtatcctaga aaatagctct
     2941 gtagtttcgt agtaggaaaa gagctcggta ggaccgtact ggtcattttc tgtcggcaac
     3001 ttttctcccg aaccatctgc atcagcaata accttgaagg catgaaaacc gggttcaggc
     3061 attgttattc cttccagcaa gagcataacc actgagcctt ctgctgccgt gacatcggtt
     3121 acccgtactt ctcttccttc attctggata ttatcagggt ccagccatat tatttctgta
     3181 tcccttccgg taccactgat agctacccag tctctgcccg ctacagcctt aatccccgct
     3241 ggcagctgaa agatcacgga accctttgtg aaatcatcac caagtgagta aaagaggacg
     3301 attctgccgc ttgagccgct gtcaactatc cccggtttta tttccaagtt tcctggtttg
     3361 gtgatttttt ctgataaaaa gctgaaaata cttttggcat tatttagaac ggtgatggct
     3421 tcagccctgg tgatatttcc ttgaggtcta aatgaaccat caggatagcc gcttaacaat
     3481 tcattgtgga cagcgactgc cacaaaagtt ctagcccaat cgggtatatc ttcgtaatca
     3541 gagaatttgg tcaaaactga ctcgtcctga ggtaaaggca attcagcaat aattttaact
     3601 aagatgacag tcacttccac tcggcttatc tgctcgtcag gacggacggt cccatcgtcg
     3661 aaacccgaaa tgtatcctgc agcaactgcc ttagccacat cttccgcata catatgatcc
     3721 tcatgcaggt cgggaaagtt aacctgtgta gttttttcaa atccaaaggc tttattagta
     3781 agtgctataa actctcctct tgtaatcttg gcatcagggt agaacttacc gtcatcaccg
     3841 tactgggtcc atcctttatc aagccactcc ataatttgct cttgtgccca gtgaccttct
     3901 acatccgatg gtttagcaaa ggatataccc gcttgcaaca aaaataccat cataatcgcc
     3961 agtgctagtg cctttttaaa ttttgacatc ttactacctc ctagcaggtt ttttgttaac
     4021 tacataataa ggtaaatcag aggacaatta aaatcatgta aaaatattta tgacatccac
     4081 gctttttact atgctcttat acaacactaa ttaacgattg ccggacctgc tacttttttc
     4141 tcatacgaca gatccggcat tattattttt agtccttacc agcccgcgac tctctcgact
     4201 tcaacatcaa acacttgacc gttccggttt atcgttagcg taacctttgt tcctgcctta
     4261 ccagatagtc ttaaccaggc ttcatcttca ccatatttca tgtcgcttat atataattcg
     4321 tctattgcca ctataatatc tccaggcata aggccagctt tgtcagcagg cattccgggc
     4381 acgcagcctt gtattactat ctcattctta tcattttcat atccgaaaat acctatttta
     4441 ccattgatta gttcatgcca tgccatctca tcatcctggt tccacaggaa ttcaacgtaa
     4501 caaatcttat catctgtcac aaaatacttg ctgacatagc ggaaaccctt atagctttca
     4561 tcagggtaca attcattaat caatacatcg gtatagaaac tctcctcttg tgatttttgc
     4621 atcgggataa aatctcccat cttcgccaat ctctgcccag cctttttcaa gccacgccat
     4681 gattttttcc tgtgcccaat gtccttctat atccgttggc tgggcaaagg aaataccagt
     4741 ttgcaataaa aacaccatga taattgctaa ggccactgaa atcttcttaa tctttgacat
     4801 ttcaaacctc ctagccttta tatttgtaaa agtagcgaaa aactaaagaa aagtttactg
     4861 tgtaaaactt tattaaaact attcacctcc ccgttttttc taaaattacc ttcagtatct
     4921 ttacttaaat aactgtcgga cctaccgctc ttttttcagc gataggtcca ataactaagt
     4981 ttatactcga tttaaaggat ttatttggaa ccgtagatca tggtgtatat cctgtacagg
     5041 aataccgctg cttcggccct tgaggtgtta tctaggggat ttattctgtt ttcactgcca
     5101 actatcaatc cttctttcac caaagatgca atgctgtcaa tcgcatattc tgctatcaag
     5161 gatttgtcct caaacctgtc taggtcggaa tcgccaccat gagtttcgag tttctttaac
     5221 atcctcaagg ccctttcggc cagtaccatc aaatcttgtc tcgtgatgct cgcattaggg
     5281 ctgaacttgt tgtttccgat acctttggca attccaatct ttttagcaat gccttattcc
     5341 ttgtagtaat aagctcctat ttctatgtca tcgaagtttt cgtcaaagct tgccgtaact
     5401 cctaatgtcc tcaccagcga gtagaggaat tctgccctgc tgatgtccgt ttgcggtgcg
     5461 tattccgtct ccgtctttcc tcttatgatt cctttagatg ccaaaacttc aatggctttc
     5521 ctggcccaag gcactgtatc cagatcatca aaggcctttg ttacgtatac aactgcaaac
     5581 tggctgaaat gggtgatgct gaagattaca gaagatgttt cagggtcata ccgccctgaa
     5641 ggaacttcta ttatattgcc tttactgtcg atgtaccaaa cggtaatgtg ttctgggctt
     5701 cttagttctt cttccgtcgg tgtatagggt atggctacgg tcacaggcac atcgtcattg
     5761 ctccaggaat atgattttcc gtcaactttt agattaagct ctatgaccgg cctgtcaccg
     5821 attctttgct gcaattcctg agaaagtttg ctcttatctg ccaaggcaat gctcatggaa
     5881 acggtttttg ctccggttag ttcttccggc agcatgttgc cgggtaaagt tattgatcct
     5941 atagcggtat ttatatttat gtttgcatct cctgcagtta atgcaccggg aggtagtatc
     6001 atttcataag cttttgcccc ttcgctctta gtcatgtcta tttctaacag cttgattccc
     6061 tgttcattgg gaacaatttc atctatggct
//
LOCUS       DAONUO010000100         6045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9770_length_6045_cov_3.310684, whole genome shotgun sequence.
ACCESSION   DAONUO010000100 DAONUO010000000
VERSION     DAONUO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9770_length_6045_cov_3.310684"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PK768_04845"
     CDS             complement(<1..567)
                     /locus_tag="PK768_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HQA60329.1"
                     /translation="MEEVVGLLKQIAPEITATVENRYTILRSIYFNQPIGRRALSSLL
                     SWPERQLRAEVKELEELGLLRINQQGMKLTGAGKDMIFKMEHFIYHLRGLSPIAQRIR
                     ELYKCRYVSIVPGNTDEDETVKLQLGKEAARYIKKTINDGDIVAVTGGTTMAQIPKAM
                     DKISGLKDVMVVPGRGGLGERVEIESNTI"
     gene            complement(657..1670)
                     /gene="gap"
                     /locus_tag="PK768_04850"
     CDS             complement(657..1670)
                     /gene="gap"
                     /locus_tag="PK768_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQA60330.1"
                     /translation="MSVKVGINGFGRIGRNALKAAFNKHPEIEVVAINDLFDTKTLAH
                     LLKYDSVFGTFDAEVEAKDSSIIVNGKEIKVFAERELANIPWKDLGVEVVVESTGVFT
                     AREKAVGHIEAGGAKKVIISAPAKGEDITIVMGVNEDKYDPENHHVISNASCTTNCLA
                     PVAKVLMDNFGIEKGLMNTIHSYTNDQRILDLPHKDLRRARAAALNIIPTTTGAAKAV
                     ALVIPELKGKLNGFALRVPTPTVSIVDLTCQLSKSATAEQINEALKEAAEGKMKGILG
                     YTEEPLVSMDFKGSELSSIVDGLSTMVIEDDLVKVVAWYDNEWGYANRVMDLVDYIAA
                     KGL"
     gene            complement(1983..3371)
                     /gene="rpoN"
                     /locus_tag="PK768_04855"
     CDS             complement(1983..3371)
                     /gene="rpoN"
                     /locus_tag="PK768_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQA60331.1"
                     /translation="MQVNFSLTLSQTQKLVMTPELRQAITILQLSTFELGQYIENQLL
                     ENPLLDLNDDFIKSETPVEREADDREDSIDWEEYFQEEAGSGPIRGPREQREEVMYEN
                     FISSTPSLQEHLMMQLRFAFISKTELKIGEFLIGNLDKNGYLSITVDEVAKLLKVSKK
                     DVEDTLKIIQTFDPAGVGARNLIECLLIQIEQNGIKAPKIKELVTNHLEDLAECRFSK
                     IAEALNISMAEVQQLKDILVTLDPKPGRNFSSINDVQYIIPDAVIEKVGNEYIVIMND
                     SISPKLSINPYYRALLTSEDKESSICKFLSNRLDSALWLIKSIEQRRITLQKVIESIV
                     RVQREFFDNGLIYLKPLTMREIADMVGVHESTVSRAISGKYVQTPRGVFELKFFFQSG
                     LENVNGTMTSAESTKKLIREIIEGEDSYNPLSDQKIADILKQKGISISRRTVAKYREE
                     MGILSSNKRKRY"
     gene            complement(3578..4081)
                     /locus_tag="PK768_04860"
     CDS             complement(3578..4081)
                     /locus_tag="PK768_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HQA60332.1"
                     /translation="MVITVVGVFDSRDQAERAVAELRQSGFDTNEISIVAKEQQGQDT
                     QADTTMGMGMGTVADGTTTGGVLGGLAGLAMGAGALAIPGLGPVIAAGPIAGLLSGAA
                     TGGIAGGLIDWGIPEEQGRKYEEEVKKGKILSAVRAHEGKVDAAAEIFRRNGARDVNT
                     QQKGSMK"
     gene            4222..4791
                     /locus_tag="PK768_04865"
     CDS             4222..4791
                     /locus_tag="PK768_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcC"
                     /protein_id="HQA60333.1"
                     /translation="MTEKGKRDKSMAEISENLGTDTANKLQEIIGEMQRGQKSPYRDP
                     MQQQFIEEVNCLLNSVRQQQLSSQSQLQASLNQATSNLTDSNRFDTILDSVKELLQAA
                     QLGVSNLQINMKQYKSIIDKMERDCHQQQVTTDLQVVQALQQAISSMAQAQNSLLQSQ
                     AVDKMYDSITKCQDKLAQIEALQSTKTTM"
     gene            complement(4836..>6045)
                     /locus_tag="PK768_04870"
     CDS             complement(4836..>6045)
                     /locus_tag="PK768_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQA60334.1"
                     /translation="CCHDIDKDGVHEIIVGCTRGILVYKMRQGKLVKIAESAELKEKV
                     TCIAVADIDKDGRHEIIAVVKGKPRIYIFRYEKRLVLIKVETIKHPVSFVACGDTDGD
                     GFPEMVVKCHGRHGCTLFVLSFKGGKMHEKWSSHITDAGDAFLRVDDFDRDGKHEIVL
                     DCSPSKIRVLRCSGNGYQSFWESPKFSESVKDVAIYDIDGDGKKELIVVCLSYVYIYS
                     WKAGKIIMEWQQTVPNGAFCVTAGELNQKGYGEIVVGTIYGYIYVFEARRERHHGKLW
                     MGKVQAIIQDTVTIPAGKPDAARGVEAKARFSVDKVKVVHDKVIVDGEVAAKVLYVAA
                     LPSQPVHFFEATIPFLEFIHLHGASPGMQALVYFNVEHISVDVVSPRRVKITILFEML
                     VKLVPFFHDP"
BASE COUNT         1553 a         1469 c         1185 g         1838 t
ORIGIN      
        1 tattgtattt gattctatct ccactctttc ccccagacct cccctgcctg gcaccaccat
       61 gacgtccttt agacctgata ttttgtccat ggccttcggt atctgggcca tagtcgttcc
      121 tcccgttacc gctacaatat ctccatcgtt tatggttttc ttgatatatc gagcagcttc
      181 cttgccgagt tggagcttta cggtttcatc ttcatcggta tttcccggca ctattgaaac
      241 atatctgcat ttgtaaagct cccttattct ttgtgcaatg gggctgagac cccgcaaatg
      301 atatataaaa tgctccatct tgaagatcat gtctttacca gcaccggtaa gtttcatgcc
      361 ctgctggttt atcctcaaca gccccagttc ctccaattcc tttacttcgg ctctgagctg
      421 ccgctcaggc caagaaagaa gggatgaaag ggctctcctg cctataggtt gattaaagta
      481 gatacttcgc aatatggtgt aacggttttc tacagtcgct gtaatttccg gagctatttg
      541 ttttaataat cccaccactt cttccattat taatctcctc aaatagactg tatatgaaag
      601 aaatggctga aaaaccctgc ctaaaggcaa gagtttatca gccatggcca tctagcttat
      661 aatcctttgg ctgcgatata atctactaag tccattaccc tatttgcata accccattca
      721 ttgtcatacc aggcaactac tttaaccagg tcatcttcaa ttaccattgt tgacaagcca
      781 tcaactatgg atgagagttc ggatccttta aagtccatgg ataccagcgg ttcttcggta
      841 tagcctaata tgcccttcat cttgccttca gccgcttctt ttaaggcctc attaatttgt
      901 tctgcagttg cgctcttgga aagttggcag gtcaggtcaa ctatggaaac cgtaggtgta
      961 ggcacccgaa gtgcgaatcc attgagcttg ccttttagtt ccgggattac aagagccact
     1021 gcttttgcag cacccgttgt agttgggatg atgttcaagg ctgcagcacg agctctgcgc
     1081 aaatccttat ggggcaggtc cagtattcgc tggtcgttgg tgtatgagtg aatggtgttc
     1141 atgagacctt tttcgattcc aaaattgtcc atcagcactt tggcaacagg tgccaggcag
     1201 ttggtggtgc aagatgcgtt agagattacg tgatgatttt ctggatcgta cttgtcttca
     1261 tttactccca taactatggt gatatcttcg cccttggcag gagcgctgat aattaccttt
     1321 ttagctccgc cggcctcgat atggccaact gccttttctc ttgcggtgaa gacacccgtt
     1381 gattcgacta caacttctac tcctaaatct ttccagggaa tgttggccaa ttctctctca
     1441 gcaaaaacct taatttcctt gccgtttacg ataatagaag agtctttcgc ctccacctct
     1501 gcatcgaagg tgccaaagac cgaatcgtac tttaagaggt gggctagggt ctttgtgtca
     1561 aataaatcgt tgattgctac tacttcaatc tccggatgct tattgaatgc agctttaagt
     1621 gcattcctac ctatgcgccc aaaaccgttg atacctactt ttactgacat aaaaaattcc
     1681 tcctttttat gaatttattt acatttaatt tgaaccggag ccgggtttag atttgtatat
     1741 agttagaatt tacttgccag accttattct attcaaaatg aatgttgaaa ttggaaagct
     1801 ccggcaaagg gacattttct gtcccttgta tttatttatt gtccctcttg ttttaaaaaa
     1861 acagggtttt tgcatttgca tcatacaaaa acacctaaaa ttagtattat tggggaaaat
     1921 tgctgatatc cttatatcat attcaacaaa acttgctaaa atccttccgc aagataaagc
     1981 ttttaatatc tttttctctt gtttgacgaa agtatgccca tttcctctct gtattttgct
     2041 acggtcctgc gggatatgga aatgcccttt tgctttaaaa tatcggctat cttctggtca
     2101 cttaagggat tgtacgaatc ttctccctca attatttcac ggattagttt ttttgtgctc
     2161 tccgctgaag tcatggtacc gtttacattc tccaacccgc tctggaagaa aaacttcagt
     2221 tcaaaaacgc cccggggggt ctgcacgtat ttgccgctga tggcccgaga tacggttgac
     2281 tcatgaactc ctaccatgtc agcaatctct ctcatggtca gtggttttag ataaattaag
     2341 ccattgtcaa aaaactccct ttgaacacgg actattgatt ctatgacttt ttggagggtt
     2401 atccgccgct gttcgatgct cttaataagc catagtgcag aatccaggcg atttgataga
     2461 aacttgcaga tacttgactc cttgtcttca gaagtaagca atgcgcgata ataagggttg
     2521 atggaaagtt tcggtgatat ggaatcattc ataatgacta tatattcatt gcccactttc
     2581 tcaataactg catcggggat gatatactga acatcgttta tcgaagaaaa atttcgtccg
     2641 ggtttaggat cgagggtaac taaaatatcc tttaactgtt ggacctcagc catgctgata
     2701 tttaaggctt ccgctatttt ggaaaaccgg cattctgcca gatcctccag gtgattggtg
     2761 accaattcct ttatttttgg cgctttaatg ccgttttgtt ctatttgtat cagcaaacat
     2821 tcaatcagat ttcttgctcc tacccctgca gggtcaaaag tttggattat cttaagcgtg
     2881 tcctctacat ctttttttga cactttcagc agctttgcta cttcatctac agtgatgcta
     2941 aggtaaccgt ttttgtctaa gttgcctatc aagaattctc caatctttaa ttcagttttt
     3001 gatatgaagg caaagcgcag ctgcatcatc aaatgttcct gcaaagacgg tgttgatgag
     3061 ataaagtttt catacatcac ctcttccctt tgttccctcg gtccccttat ggggccgctg
     3121 cctgcctcct cctggaaata ctcttcccaa tcaatactgt cttccctgtc atcggcttcc
     3181 ctttcaacag gagtctcaga ttttataaaa tcatcgttca aatcaagaag cgggttttct
     3241 aaaagctgat tctctatata ctgtcccaat tcaaaagtgg aaagctgcaa aattgttatg
     3301 gcttgtctga gttcgggagt cataaccagc ttttgagttt gagaaagtgt aaggctgaaa
     3361 tttacctgca tctttttccc cccttgggaa aacttcatat ttaatagccc taattaaaac
     3421 tgtgttatat atatattcta taaaaatatc aatttttcct ctttttttaa aacaaatgga
     3481 atgaaatttg ctagagaatt tgcccgtttg tgtcataggg atgaataaat agaggcagga
     3541 cgtctcttga acattcaccc tgcccctatt aattatttta tttcatggag cctttttgct
     3601 gcgtatttac atcgcgtgcc ccatttcgcc tgaaaatttc agccgcagcg tccacttttc
     3661 cctcatgggc cctgacggct gacagtattt tacccttttt cacctcttct tcatattttc
     3721 gaccctgttc ttcagggatg ccccaatcga tgaggcctcc ggcaataccg cctgttgcag
     3781 cacctgacag caagcccgca ataggtcctg ctgcgataac aggacccagg cccggtattg
     3841 ccaaggcacc ggcccccata gcaagaccgg caaggccacc gaggacacca cctgttgttg
     3901 tgccatctgc caccgtgccc atacccatgc ccattgttgt atcagcctga gtatcctggc
     3961 cctgttgctc tttggctaca atcgatattt cattggtatc aaagcccgat tgtctgagtt
     4021 ctgctacggc cctctctgcc tggtcgcgac tgtcaaagac tcccacaacg gttattacca
     4081 ttattttcta cctcccggct gtatttttaa atcactctta ttttatcctt gttttaaaac
     4141 agctattccg tgcatgataa attccaataa agggaatagt aatataaaaa tatgtctccg
     4201 ggggtgtttg ctttgacaga tgtgacggaa aagggcaaaa gggacaagag tatggccgaa
     4261 atcagtgaaa acttgggcac cgacaccgca aataagcttc aggaaataat aggagaaatg
     4321 cagcgggggc aaaagagtcc ctacagagat ccgatgcagc agcagttcat agaggaagtt
     4381 aattgcctgc tgaattccgt gcggcagcag cagctctcct ctcaaagcca gcttcaagcc
     4441 tcactaaatc aagcaacgag caatttaact gattccaatc gatttgacac gatattggat
     4501 agtgtcaaag agcttctgca ggcagcccag ttaggcgtct ccaatcttca gataaatatg
     4561 aagcagtaca agagcatcat agacaaaatg gaaagggatt gtcaccagca gcaggtgacc
     4621 acggatctcc aggtggtaca ggccttacag caggccatat cttcaatggc ccaggcgcaa
     4681 aacagcctcc tgcaaagcca ggcagttgac aagatgtatg actcaattac aaaatgccag
     4741 gacaagctgg cacaaattga agccttgcag agcacaaaga ctaccatgta aatctacata
     4801 aaaaagcggg cggtcactcc gcccaaaatt actttttaag gatcgtggaa aaaaggcaca
     4861 agttttacca gcatttcaaa taaaatcgtt attttaaccc ttctgggact taccacatca
     4921 acgctaatat gttctacgtt aaaataaact aaggcctgca tgccgggact tgctccgtgt
     4981 agatgaataa actccaagaa tggaattgta gcttcgaaaa agtgaaccgg ttgcgagggc
     5041 agagccgcca catacagcac ttttgctgca acttctccat ccacgattac tttatcgtgc
     5101 accaccttta ctttatctac ggaaaacctg gccttagcct caacgcctcg agctgcatcg
     5161 ggctttccag caggtattgt aaccgtatcc tgaattatgg cctggacttt gcccatccaa
     5221 agtttgccgt ggtgtctttc gcggcgggct tcaaatacgt aaatatatcc gtagatggtg
     5281 cctacaacta tctcgccata tcccttctga ttcagttcac cggcagtaac gcagaaagct
     5341 ccgtttggaa ccgtctgctg ccattccatt atgattttcc cggccttcca gctgtatata
     5401 tagacatagc tcaggcagac aactatcaat tcctttttcc cgtcgccgtc aatatcgtat
     5461 atggccacat ctttaacact ttcactaaac tttgggcttt cccaaaagct ttggtagcca
     5521 ttcccggagc agcgcagcac ccttatctta cttgggctgc agtcaagcac tatctcatgt
     5581 tttccatccc ggtcaaaatc atcgaccctc agaaaggcat ctcccgcatc tgttatgtgg
     5641 ctgctccatt tttcatgcat ttttccgccc ttaaaggata atacaaacag ggtgcagccg
     5701 tgcctgccat ggcatttgac taccatctcc gggaagccat cgccatcggt atcgccgcaa
     5761 gctacaaagg aaaccgggtg ttttatcgtc tctactttaa taaggaccaa tctcttttca
     5821 tagcgaaaaa tataaatgcg gggtttgccc tttactaccg caattatctc gtgtctgcca
     5881 tccttatcga tgtctgcaac ggctatgcag gtcacctttt cttttagttc cgcactttcc
     5941 gcaatcttca caagcttgcc ttgtcgcatc ttgtaaacga gaatgcctcg cgtgcagcca
     6001 acaattattt catgaactcc atccttgtca atgtcatgac agcag
//
LOCUS       DAONUO010000101         6035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9789_length_6035_cov_3.384783, whole genome shotgun sequence.
ACCESSION   DAONUO010000101 DAONUO010000000
VERSION     DAONUO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9789_length_6035_cov_3.384783"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PK768_04875"
     CDS             complement(<1..439)
                     /locus_tag="PK768_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HQA60335.1"
                     /translation="MIEKLEAVEQKYIELTKKIADPEVIAKQEEWRKLAKEHASLEEI
                     VNCFREYKKATKQIEENNELLKEASGDDEMEELIKSDIESLEEKRKKLEEELKILLLP
                     KDPNDEKNVIVEIRAGTGGEEAALFAGDLFRMYNRYAEKRGWKA"
     gene            complement(465..1322)
                     /locus_tag="PK768_04880"
     CDS             complement(465..1322)
                     /locus_tag="PK768_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HQA60336.1"
                     /translation="MKPVCPKIGGQAVIEGVMMRGPKTTAIAVRKNDDIIIKTQENHS
                     VQDKYKFLKLPILRGIVSLVEMLVLGIQVLSYSASVAGLDDEEELTGKDIAFALISAF
                     AFAILLFIVLPTIAVRFISGNLQNPFLLSLAEGLLRIAIFVIYIAAISTMKDIRRVFE
                     YHGAEHKAVHCLEHNDKLTPENAKKYTTIHPRCGTSFMMIVMVVSILLFSFIGWQGLL
                     LRILSRIVLLPVIAGISYEFIRLAGESESIFVKVLNTPGMWLQRLTTREPDESQLEVA
                     IAALKSVIS"
     gene            complement(1386..1595)
                     /gene="rpmE"
                     /locus_tag="PK768_04885"
     CDS             complement(1386..1595)
                     /gene="rpmE"
                     /locus_tag="PK768_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQA60337.1"
                     /translation="MKKGIHPNYVRATVRCACGNTFETGSTKEDLRVEICSKCHPFFS
                     GQQKLVDTGGRVERFKKRYGLKDAE"
     gene            complement(1750..2973)
                     /locus_tag="PK768_04890"
     CDS             complement(1750..2973)
                     /locus_tag="PK768_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA60338.1"
                     /translation="MFEPLWGIPYVLYNFYLSLYMKSMGITDKQIGFLISLGFISAIV
                     FSLMAGVITDALGRRKTTLIFDLIGWPCSILLYAFAKDFWMFALAIIVNGAHRIPAVS
                     FNLMVIEDADNTQRFAAFNLINIINISAGLLTPIAGLAVRMYGIVNAERFLLFFAATS
                     MTLMMIMRYRYHTETRIGQKILQERKKHTKKRGIQKEAYKKVLGDLVKKPTVLMIMSV
                     VILFNIYMTIGTHLSLYFAPYMSEVLGIEKSMMSLLGVANSATMITTLVFINPLISGE
                     NKMKNMMLALVLQALSLFLLTIISGNNFMIVVCCIMLFAVGFGIFKPSIDSLLAELTE
                     GDSRAIIYSLVNTSISASSALMGFVSGYIYDLNPRLIYLTSITILAICLLILIALKRS
                     LDHEYEDVEFTDAAL"
     gene            complement(3145..4134)
                     /gene="amrS"
                     /locus_tag="PK768_04895"
     CDS             complement(3145..4134)
                     /gene="amrS"
                     /locus_tag="PK768_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQA60339.1"
                     /translation="MREAMYYEQAAGGAVNCALCPHRCRIAAGRVGTCKVRKNVDGKL
                     YSISYGRISSWAVDPIEKKPLYHFYPGRMIFSVGSIGCNFRCKFCQNWQIAQLTDIPT
                     QEVSAEELVWVAKRQAGNIGIAYTYNEPTIWYEYVLECVKLARQQGLKNVLVTNGFIE
                     KEPLEELLPYIDAMNIDVKAFHEDFYKDLTFGRLSPVKQTVEIAQDKCHVEVTTLIIP
                     GMNDENEEIEELARWLSSLRRDIPLHLSRYFPNYKLDLPPTPVDRIKTAWDIAKKYLE
                     YVFTGNIIDDVGSNTYCPSCGKLVIRRSGYITHSNVKDNKCPRCKAYISITED"
     gene            complement(4324..4887)
                     /gene="ahpC"
                     /locus_tag="PK768_04900"
     CDS             complement(4324..4887)
                     /gene="ahpC"
                     /locus_tag="PK768_04900"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HQA60340.1"
                     /translation="MALIGKEVSDFTVKAFVDGEFKDVSKADLLGKWSVFVFYPADFT
                     FVCPTELEDLADIYDEFKKIGVEVYSVSTDSHFVHKAWHDASETIKKIKYPMLADPTG
                     KLCRDFEVYIEEEGMALRGSFIINPAGKIVAYEVNDNSIGRDASELLRKVQAAQFVAE
                     NGDQVCPAKWRPGSKTLKPGIDLVGKI"
     gene            complement(5171..>6035)
                     /locus_tag="PK768_04905"
     CDS             complement(5171..>6035)
                     /locus_tag="PK768_04905"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQA60341.1"
                     /translation="TSDAAGLCIKSGNAVILRGGSEAINSNMAIVNALRKGLEEADLP
                     VSCIQLIEDTSRERIQELFKMRDYVDVLIPRGGAGLIKNVVENSQIPVIETGVGNCHL
                     YIDETADLEMAKNILINAKTNRPSVCNAAETLLVHEKIAADFLPGALDELKELGVEIR
                     ACERTLNIYPDAKSATEEDWSTEYLDLILAVKIVDSIEEAIAHINRYGTSHSEAIVTK
                     DYERANQFLARVDAACVYVNASTRFTDGGEFGMGAEIGISTQKLHARGPMGLEELTTV
                     KYLIMGSGQIR"
BASE COUNT         1536 a         1401 c         1230 g         1868 t
ORIGIN      
        1 ccgctttcca gcctcgcttt tccgcatagc gattgtacat gcgaaacagg tcaccggcga
       61 agagggccgc ttcttcaccg ccggtcccgg ccctgatttc aacaatgacg tttttctcgt
      121 cattggggtc cttgggaagc agcaagattt ttaattcttc ctccaacttt ttcctttttt
      181 cttccaagct ctctatatcg ctctttatca gttcctccat ttcgtcatcg ccggaggctt
      241 cctttagcag ctcattgttt tcctctatct gcttggtggc ttttttgtat tccctaaagc
      301 aatttacgat ctcttcaagg gaagcatgtt ccttagccag cttcctccac tcttcctgtt
      361 tggctatgac ttcagggtct gcaatttttt tcgtcaattc aatgtatttt tgttctacag
      421 cttcaagctt ttcaatcaaa gcaatcacct tcctactaca agactcagct tattacgctc
      481 tttagtgcgg caatagctac ttctaactgg gactcatccg gttctcgggt agtcagtcgc
      541 tgaagccaca taccgggagt attcaatact tttacaaaaa tgctctcgct ttcaccggca
      601 agcctgataa actcatatga aatccccgct ataacgggta gcaagacaat tcttgagagt
      661 atccttagta gtagtccctg ccaaccgata aatgaaaaga gcagaatgct tacaaccatg
      721 actatcatca taaagctggt gccgcatctt gggtgaatgg tggtatattt tttcgcattt
      781 tccggcgtca gcttgtcatt gtgttcgagg cagtggactg ccttatgttc tgcaccgtgg
      841 tattcaaaga ctctccttat atccttcatc gtagaaatag ccgcaatgta tattacaaat
      901 atggctattc tcaaaaggcc ttcagccagg ctcagcaaaa atgggttttg caaattgccg
      961 cttataaacc ttacggcaat ggtaggcagc actatgaaaa gcagtatggc aaaggcaaag
     1021 gccgaaatca gtgcaaaggc tatatccttg cccgtgagtt cctcttcatc atctaatccg
     1081 gcaacgctgg cggagtaaga gagtacctgg atgccaagga ccagcatctc taccaatgaa
     1141 acaatacctc tcaatatggg tagttttaga aatttatatt tatcctgtac agaatggttt
     1201 tcctgggttt ttatgattat gtcgtcattt ttcctgacgg caatggctgt agtcttcggg
     1261 ccccgcatca ttacgccctc gattacggcc tgccctccta tttttggaca tacgggtttc
     1321 atggatttcg cccccaatac aaaagtgaag aaaaaaggtc agagataacc tctaaccttc
     1381 ttcgactact ccgcatcttt taagccatat ctcttcttga atctctctac acgtccaccg
     1441 gtatctacca gtttctgttg gcctgaaaag aagggatggc actttgaaca tatttcgaca
     1501 cgcaggtctt ctttggtaga ccccgtttca aatgtgtttc cgcaagcaca tctgactgtg
     1561 gctctcacgt aattgggatg gattcctttt ttcacgctgt attcacctct ttattataag
     1621 ttatgcaaat ttgttcttat caagtgccat tttgctttct acagtataaa agcattacgg
     1681 catttttaca cttagacttt tgaagtcttt tagtattata gcacaggcta tatttttaca
     1741 caagagtttt tataaagctg catccgtaaa ctccacgtct tcatactcat ggtccaatga
     1801 ccgtttaagg gctatcaaaa taagcaggca gatggctaaa atagttatag aagttaagta
     1861 tatgagtcgc ggatttaaat catatatgta acccgacaca aaacccatca aagcgcttga
     1921 tgcggaaatg ctagtattta ccaatgagta gattattgcc ctgctgtctc cctcggtgag
     1981 ctctgccagc agtgagtcta tggagggttt aaagatgcca aaacccaccg caaatagcat
     2041 gatacagcaa actactatca taaaattgtt gcccgatatt atcgtcagta aaaagagtga
     2101 aagggcctgc agtactaggg caagcatcat gtttttcatc ttgttttccc cgcttattaa
     2161 agggttgata aagaccaggg tggtaatcat ggttgcggaa ttggccactc caagtaggga
     2221 catcatggat ttttcaattc caaggacttc cgacatataa ggggcaaagt acaggctcaa
     2281 atgggtgcca attgtcatat aaatgttaaa cagtataaca acactcatta tcattaagac
     2341 cgtaggcttt ttaacaaggt cccccagcac tttcttgtat gcttcttttt gaatgcctcg
     2401 ttttttagtg tgcttttttc gctcctgcag tattttttga cctatccgag tttcggtgtg
     2461 atagcggtag cgcataatca tcatcagcgt catgctcgtt gcagcaaaaa atagcaaaaa
     2521 tctttccgca ttcactatcc cgtacatcct aaccgccagt cccgcaatag gtgtcaaaag
     2581 gcctgccgaa atattgatta tattgatcaa attaaaagct gcaaagcgct gtgtattgtc
     2641 ggcatcttct attaccatca ggttgaatga aacggcgggt attcggtgag cgccgtttac
     2701 tattattgcc agggcaaaca tccaaaagtc cttggcgaag gcatacaaaa gtatggaaca
     2761 aggccagccg ataaggtcga aaattaaggt cgtcttcctc ctgcctaagg catccgttat
     2821 gacacctgcc atcagggaaa atacaatagc tgatatgaaa ccgagagaaa ttaaaaatcc
     2881 tatctgcttg tcggtaatac ccatactctt catgtagagg ctcagataga aattgtagag
     2941 cacatagggt atgccccaaa ggggttcaaa cattacgcta actttggcgt ttccatccag
     3001 tattttaaaa gattctatga tatttgtagg tttgctattt atgtcttttt ctcctctcta
     3061 tttattttca aactttcggc agaatctcat catcctttcg acaccgaagg tagcatttcc
     3121 cgggaaatag acaccgcagg cttcctagtc ctcagttata gatatgtaag ccttgcaacg
     3181 gggacatttg ttgtctttta cattcgagtg cgttatatag cccgacctcc tgattacgag
     3241 ttttccgcag ctagggcagt aagtattgct ccctacatca tctattatat ttccggtgaa
     3301 aacatattca aggtatttct ttgcaatatc ccaggccgtc tttatcctgt ctacgggagt
     3361 tgggggaagg tccagtttgt aattgggaaa atagcggctt agatgaaggg gtatgtccct
     3421 tctaagggat gaaagccatc ttgccagttc ctcgatctcc tcgttttcgt cgttcatgcc
     3481 gggaattatc agagtggtta cttcaacatg gcatttatcc tgtgctattt caacggtttg
     3541 ttttaccggg gaaaggcgtc cgaaagtcag gtccttgtag aagtcctcgt ggaaggcctt
     3601 tacatcaatg ttcatcgcat cgatataagg gagaagctcc tccaggggtt ccttctctat
     3661 aaagccattg gtcacaagga catttttcag gccttgctga cgtgcgagct ttacacactc
     3721 taagacgtat tcataccata tggtgggttc attgtaggtg taagctatgc ctatgttgcc
     3781 tgcttgtctt ttggctaccc aaaccaattc ctctgcagaa acttcttgtg taggaatatc
     3841 ggtaagctgg gctatttgcc agttttgaca aaatttgcac ctaaaattgc agcctatgct
     3901 gccgactgaa aaaatcatgc ggcccgggta aaagtgatag aggggctttt tttctatagg
     3961 gtccactgcc caggaagata tcctgccgta actgatggaa tacagcttac catcaacatt
     4021 ttttcgcacc ttgcatgtac cgactctccc tgctgcaatc ctgcagcggt gggggcacaa
     4081 agcgcagttg acagcaccgc ctgcagcttg ctcgtagtac atggcttctc tcatggggtt
     4141 ttgctggcct ccttgctctt ttatggggca taatttcgat tcaatagtcg ccggtacatc
     4201 attatacctt taacggcaag gctatttcaa gcttatgggc ttaatgaaaa cacctttctt
     4261 tttgaaagta aaagaaaggt gttttttgtt tcaggatgaa gtattcaact tggtccttga
     4321 taattaaatc ttgcctacca ggtcgatgcc gggtttcagg gttttggaac cggggcgcca
     4381 ttttgcaggg cagacttgat cgccgttctc cgctacgaac tgggcagctt gaactttgcg
     4441 caggagttcg gaagcatctc tgccgatgct gttgtcattt acttcataag ctactatctt
     4501 acctgccggg ttgattataa agcttcccct gagagccatt ccctcttctt ctatatacac
     4561 ttcaaagtct ctgcagagct ttcctgtggg gtctgcgagc atggggtatt taatcttctt
     4621 aatggtttct gaagcatcat gccaagcctt gtgtacaaaa tgactgtccg tggataccga
     4681 atatacctct acacctatct ttttaaactc atcatatata tcggctaaat cctccaattc
     4741 tgtggggcat acgaaagtga aatctgctgg ataaaacaca aacaccgacc acttgccgag
     4801 taaatccgcc ttgctgacat ccttaaattc tccgtcaaca aaagccttca ccgtaaaatc
     4861 tgatacttct tttccaataa gtgccataat gaagcctcct tctagcacaa atattttttt
     4921 attttaccgg ttaaacaccg gtgattgaca aactgcctta tatgtactgt ttaacatgct
     4981 attgcgagcg caattgataa tagttattat tattataact gtgtattact aaattgtcaa
     5041 cagttatttt aaaacttata cacaacagca tatgattgca agaattagaa gctagactgt
     5101 aaataaaaaa agccacggta tttataacta cataatgacc gtggctattt atcgtgaaat
     5161 ttgatatcga ctatcgtatc tgaccgcttc ccatgatcaa atattttacc gttgtcaatt
     5221 cttcaaggcc catgggccct cttgcatgaa gcttctgggt gcttattcct atttctgccc
     5281 ccatgccaaa ttctccacca tcggtaaacc tcgtggaagc attgacataa acgcaggcgg
     5341 catccactct cgcaagaaac tggtttgccc tttcgtagtc ttttgtcact atagcttccg
     5401 aatgggaggt gccgtaccga ttgatgtgag caatggcctc ttctatgcta tctactattt
     5461 ttacggcaag tatcaagtcg aggtactcgg tggaccagtc ttcttcggtt gccgatttgg
     5521 catcggggta aatattaagg gtcctttcac aagccctgat ttccactccc aattctttta
     5581 gctcatctaa ggcacctggc agaaagtctg cagcaatctt ttcgtggacc aaaagggtct
     5641 cagcggcatt gcacaccgat ggcctgttgg tcttggcatt tatgagaatg ttctttgcca
     5701 tctctaaatc cgcagtttca tctatataca ggtgacaatt tccaacacct gtttcgatta
     5761 cgggtatttg ggagttttcc acgacgtttt ttatgaggcc tgctccgccc cttgggatta
     5821 agacatctac gtaatccctc atcttaaaga gctcttgaat tctttccctg ctggtatcct
     5881 ctattagttg tatgcatgat accggcaggt ccgcctcttc caggcccttg cgaagggcat
     5941 tgactattgc catgtttgaa tttatggctt cggagccgcc ccgcaggatt acggcatttc
     6001 ccgattttat gcaaaggcct gccgcgtctg aggtc
//
LOCUS       DAONUO010000102         6010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_9832_length_6010_cov_3.929471, whole genome shotgun sequence.
ACCESSION   DAONUO010000102 DAONUO010000000
VERSION     DAONUO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9832_length_6010_cov_3.929471"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PK768_04910"
     CDS             <1..800
                     /locus_tag="PK768_04910"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HQA60342.1"
                     /translation="SRMKKEPRSTQGSQLNFETIMLPKSEYNTNNFSKIYDRRTDSET
                     FIKEAPVIKKEVHVDKTHEFTPVTEATDVSKKFEYKRILGQLFNTYIVVEGQNEFYLI
                     DQHAAHERILYELFSKRYTRATETQVLVAPQVISLSPQEMMLIEYYGDDIIKLGFDYS
                     VFGTDSILVRAVPYIFNKPVDPMALRDALDRINNNEYSGFPENERLIISMSCHSAIRA
                     GDTLSQIEMEELIQQLVNTQGPYTCPHGRPTIISITINELEKMFKRT"
     gene            807..1751
                     /gene="miaA"
                     /locus_tag="PK768_04915"
     CDS             807..1751
                     /gene="miaA"
                     /locus_tag="PK768_04915"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQA60343.1"
                     /translation="MSKELLVVIVGPTAVGKTEIAIEVSRKIDAEIISADSMQIYRYM
                     NIGTAKPTLEERKGIIHYMIDIINPDEIFSAADFQARAKDCIENIHRRNKIPLMTGGT
                     GFYINSVCYDYAFSDAQRDDSFRKQLQKQARQYGNQYLYEKLEKLDPVGAKKIHPNNL
                     RRIIRAIEVSIKTGKPFSYYEEETKKQESPYDLLMYGLTRPRKELYQRINQRVVNMIN
                     AGFVEEVKRLLKMGYGPELNSMQGLGYRQIIDYLYGKTTLNEAINLISRDTRRYAKRQ
                     YTWFLRDRNIVWLDVSKEGKEKIISNIVKDIEGKLKNT"
     gene            1789..2046
                     /gene="hfq"
                     /locus_tag="PK768_04920"
     CDS             1789..2046
                     /gene="hfq"
                     /locus_tag="PK768_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HQA60344.1"
                     /translation="MTKVQINLQDSFLNQIRKENIPVTIYLINGFQLKGLIRGFDNFT
                     VFLDSDGKQQLVYKHAVSTITPFKTVCFNQGAQEQKKSDEE"
     gene            2188..3159
                     /gene="spoVK"
                     /locus_tag="PK768_04925"
     CDS             2188..3159
                     /gene="spoVK"
                     /locus_tag="PK768_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein K"
                     /protein_id="HQA60345.1"
                     /translation="MADKEKDRINNPHQIYEMLINGAITPGQAIIMFNELDNSKKPMG
                     KAVDEESLDEVMRELDQLIGLSKVKYLVKEIQAFAQIQKKRQEAKLLAEPQVLHMIFK
                     GNPGTGKTTVARLLGKMFKHMGILQKGHTVEVERADLVGEYIGHTAQKTREQIKKAYG
                     GILFIDEAYSLARGGEKDFGKEAIDTLVKAMEDYKDNLIVILAGYKDEMEWFLQTNPG
                     LRSRFPIQIQFNDYSVDELMEIAKMMVEKRQYKFSPEALITFERLLQNSKSDRNYDKL
                     GNARLVRNMIEKALRRQALRLVNQKRISREDLLQIKPEDISEVPMNQ"
     gene            3156..3764
                     /locus_tag="PK768_04930"
     CDS             3156..3764
                     /locus_tag="PK768_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQA60346.1"
                     /translation="MKQCTIIGKTNVGKTLFLISFAEYLGPKTFDIDFVYSDGTKKTK
                     RISYKSAISRLVDEQPHKTRCIQAITLNMPMGKGKKKIKIIDTAGLIDGIHPDIEVRK
                     AISQTLSIIRDSDVILHIIDAHAARNKDLPSSMGEVDYQIAQFAQLKRGYAILANKMD
                     LPEAQEGYRKIKEEFSGHVIFPVSALYKKGFKEVKTFVLRNI"
     gene            3745..4338
                     /locus_tag="PK768_04935"
     CDS             3745..4338
                     /locus_tag="PK768_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60347.1"
                     /translation="MSFETSDVYFLKKVFSILITGIVIKIMDDYFDQDIDALEKQPNI
                     YMAMEYGGLPYTLLLFSLAFMLDPITALSLFFSSFAIGMVGHLTVKMPSGLYGYHESI
                     IVVLLGFVLLKLEMLSSLFIVMVIQLWDDYLDYEKDRMNKYNLAFQLGKVECLLLAVI
                     LFLLTAYFDYIKAIASIIAMHVIVYIIRLLPDNQKPI"
     gene            4600..5895
                     /locus_tag="PK768_04940"
     CDS             4600..5895
                     /locus_tag="PK768_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HQA60348.1"
                     /translation="MSENLGFKIENIVSKEFGISPKLIKMSEMVSKEIVPLLEEIERI
                     VEFNQIKVLSAMKRSYLSDYHLTGSTGYGYSDIGRDTIDQIYADVFKAEDALVRPHIV
                     SGTHAITLCIQGVLRPGDELLSATGAPYDTLKKVLLGNHYGSLKDFGITYREASLKNQ
                     KPDYDAIRENINEKTRMVLIQRSRGYSLRPSLSIDEIEHLIKFIKSIKHDIICFVDNC
                     YGEFVEAREPIEVGADLCAGSLIKNPGGGVAPTGGYVVGKRDLVRQCAYRLTAPGLDK
                     SIGSALFSNHLIAQGLFYSPKVVGEALKGAVYTSRMMEKLGFEVSPRFDEKRCDIVTA
                     AILGEEDALISFCRGIQKAGPVDSHVQPEPSAMPGYEHKVIMAGGSFIQGSSIELSAD
                     GPLREPYAVYVQGGLNFAHVKIGIMSGLQALMDSGKLTL"
BASE COUNT         2133 a          976 c         1250 g         1651 t
ORIGIN      
        1 ggtcccgtat gaaaaaagaa ccgagaagta ctcaaggttc tcagttaaat tttgaaacta
       61 ttatgttgcc aaaaagtgaa tataatacca ataatttcag caaaatatat gacaggagaa
      121 cagattctga aacttttata aaagaagctc cggttataaa gaaggaagtg cacgttgaca
      181 aaactcatga atttactccg gtgactgaag ccactgacgt atccaaaaaa tttgagtaca
      241 aaaggattct aggtcagcta ttcaatacat atattgttgt tgaaggccag aatgagttct
      301 atttaattga tcaacatgcc gcccacgaaa gaatattata tgagcttttt tccaagcgat
      361 atacaagagc aacagaaaca caagttttag ttgcacctca ggttataagt ttatcacccc
      421 aggagatgat gctgatagaa tactatggag atgatattat aaaacttggt tttgattatt
      481 cggtatttgg aactgactcc attcttgtaa gggcagtgcc ttatatattt aacaaacctg
      541 tggatcccat ggcccttcgc gacgctcttg acagaataaa taacaatgaa tattcaggat
      601 tcccagaaaa tgaaaggtta ataatatcaa tgtcgtgtca ttctgctata agagcgggcg
      661 atactttgtc tcaaatagaa atggaagaat tgattcagca gcttgtcaat actcaaggcc
      721 cttatacatg cccccatggc agaccaacta taatatccat cactataaat gaactagaaa
      781 aaatgttcaa aaggacctaa agtcaaatga gcaaagaact attagtagta attgtggggc
      841 ctacagcagt aggaaaaacc gaaattgcca tagaagtgtc taggaaaatt gatgcagaaa
      901 ttatatctgc tgattcaatg caaatatatc gttacatgaa tataggaacg gcaaaaccga
      961 ctttggaaga acgcaaagga ataattcact acatgattga tattataaat ccagatgaaa
     1021 tttttagcgc tgcagacttt caagcaaggg ccaaagattg tatagagaat atccatagac
     1081 ggaataaaat tccgctcatg accgggggca caggttttta tataaattcc gtatgttatg
     1141 actacgcctt ttccgatgcc caaagagatg attcctttcg aaaacaattg caaaagcaag
     1201 caagacaata cggcaatcaa tacttgtacg aaaaactaga aaaactggac cctgttgggg
     1261 caaaaaaaat acatcccaac aatttacgca gaattatccg cgctatagaa gtatcaataa
     1321 aaacaggaaa acctttttca tattacgaag aagagacgaa aaaacaagaa agtccttatg
     1381 atttattaat gtacggcttg acaagaccta gaaaagaact atatcagcgc attaatcaaa
     1441 gagttgtaaa tatgataaat gcaggtttcg tagaagaggt gaagaggctt ttaaaaatgg
     1501 gatatggtcc ggaactaaat tcaatgcagg gtctaggtta tcgtcaaata attgactatc
     1561 tatatggtaa aacgactctt aatgaggcca ttaacttaat atctagagat acgaggcgtt
     1621 atgcgaagcg ccaatataca tggtttttga gggacaggaa cattgtatgg ctcgacgtct
     1681 caaaagaagg aaaagagaaa ataatatcaa atattgttaa agatattgaa gggaaattaa
     1741 aaaataccta gaatattaaa taaaacaaca atatgagggg gacccgtaat gacaaaagta
     1801 caaataaacc tgcaggatag ttttttaaat caaataagaa aagaaaacat tcctgtaaca
     1861 atttatctca ttaatggttt tcagttaaaa ggcttaataa gaggttttga taattttact
     1921 gtttttctcg attccgatgg aaaacaacag ctagtatata agcatgctgt ttcgactatt
     1981 acgcctttta aaactgtatg ttttaatcag ggtgcacaag aacaaaaaaa atcagatgaa
     2041 gaataggtat tctataaaag gagctgctgc tccgggcaaa tgttcggatg cagcagctcc
     2101 ttttaatttt attagaaata atatctgtaa cctaaactta ctgttgtgca taggataaat
     2161 tgatatggta ttttatgagg tgatcctatg gccgataagg aaaaggatag aataaataat
     2221 ccacatcaaa tctacgaaat gctaatcaat ggagcaataa ctcctggtca agcaataata
     2281 atgtttaacg aattagataa tagtaaaaag ccaatgggaa aagctgttga tgaagaaagt
     2341 ttagatgaag tgatgagaga acttgatcag ctaattggcc tttctaaagt aaaatactta
     2401 gtaaaagaaa ttcaagcttt tgctcaaatt cagaaaaaga ggcaagaagc aaaactttta
     2461 gcagaacctc aagttttaca tatgattttt aaaggaaatc caggcacagg gaagacaacg
     2521 gttgcgagat tactagggaa aatgtttaag catatgggga ttcttcaaaa aggtcatacc
     2581 gtagaagtgg aacgggctga tctggtggga gaatatattg gacatactgc tcaaaaaacc
     2641 cgcgagcaaa ttaagaaggc ttatggaggg atattgttta tagatgaagc atattcttta
     2701 gcaaggggag gagaaaagga ctttggcaag gaagccatag acaccttagt gaaagccatg
     2761 gaagattaca aagataatct cattgtgatt ttagccggat acaaggatga aatggagtgg
     2821 tttttgcaaa caaatccggg cttaagatcc aggtttccca ttcaaatcca atttaacgac
     2881 tacagtgttg atgaattgat ggaaattgcc aaaatgatgg ttgaaaaaag gcagtacaaa
     2941 tttagtcccg aagcgttaat cacatttgaa aggctattac aaaacagcaa gagtgataga
     3001 aactatgata aattggggaa tgctagactt gttcgcaaca tgatagaaaa agctctcagg
     3061 cgtcaggcac tgcgccttgt aaatcaaaaa agaatctccc gcgaagacct acttcaaatt
     3121 aaacctgagg atatttcgga ggtgccaatg aatcaatgaa gcaatgcacg ataataggaa
     3181 aaacaaatgt ggggaaaaca ctgtttttga taagttttgc agagtatttg ggacctaaaa
     3241 cttttgatat agactttgtg tacagcgatg gaactaaaaa aaccaaaaga atttcttaca
     3301 aaagcgctat ttcacgcctt gttgatgaac aacctcataa aacaaggtgt atacaggcta
     3361 taacgcttaa tatgccaatg gggaagggga agaaaaaaat aaaaattata gatactgccg
     3421 ggttaataga cggcatacat cccgatatag aagtgcgaaa agccatctcc caaacactat
     3481 cgattatacg tgattcagat gttattcttc atataattga tgctcatgct gcaagaaata
     3541 aagacttgcc cagctcaatg ggtgaagtgg actatcaaat tgctcaattt gcccaattaa
     3601 agaggggtta cgcaattttg gcaaataaaa tggatcttcc ggaagcacaa gagggatata
     3661 gaaagataaa agaagaattc tccggccatg taatatttcc tgtgtctgca ctatataaaa
     3721 agggttttaa agaggtgaaa acttttgtcc ttcgaaacat ctgacgtata cttcttgaaa
     3781 aaagttttta gtattctcat aacaggtatt gtaataaaaa taatggatga ctattttgat
     3841 caggacattg acgccctaga aaaacagccg aacatttata tggccatgga atacggcggc
     3901 ttgccttaca cattattact attttcactg gcattcatgt tggaccctat caccgcttta
     3961 agtttatttt tttccagctt tgccattgga atggtgggac atttaaccgt aaaaatgcct
     4021 tcaggacttt acggatatca tgaaagcatc atagttgttt tgttaggctt tgtcctgctt
     4081 aaacttgaaa tgctatcatc tttgtttata gtaatggtta tccaactgtg ggacgattat
     4141 ttggattacg aaaaagacag aatgaataaa tacaatttag catttcagct aggcaaagtg
     4201 gaatgcctgc tgctggccgt tattttattt cttttgactg cctattttga ttacataaaa
     4261 gctatagcat caataattgc aatgcacgtt attgtttaca taatacgatt gttgcccgac
     4321 aatcaaaaac ctatataaaa aggggagaag catatgcctt ttgataaaat aacactgatc
     4381 gcagcgctag cgattacggt ttgtctattt gggtatgctt tgggaagaag ggtcggaaaa
     4441 caagaaggaa taaaggaggg tatgagtata ttgcctctag aatggagaag acaactaaac
     4501 gaaacatctg tctgcccgct atgcagtcta cggttgaacc aacacaaaaa ttgtgataat
     4561 atacatagtc gagattgaaa gtgataaaga gggaaataga tgtctgaaaa tttaggtttt
     4621 aagattgaga atatcgtttc aaaagaattt ggcataagtc ctaagcttat taaaatgtca
     4681 gagatggtct caaaagagat tgttccttta ctagaagaaa tcgaaagaat agttgaattt
     4741 aaccaaatta aggtgctttc ggcaatgaaa cgatcctatt taagcgatta ccaccttaca
     4801 ggctctaccg gatacggtta cagtgatata ggaagagata caatagacca gatttatgca
     4861 gatgttttca aggccgaaga tgcccttgtg cgccctcaca ttgtatcggg tacccatgct
     4921 ataactctgt gtattcaagg agttttaaga ccaggtgatg aacttttaag tgccacgggt
     4981 gcaccctacg atactttaaa aaaagtattg ctggggaatc attacggctc attaaaagat
     5041 tttggcataa catataggga agccagttta aaaaaccaaa aacccgatta tgatgccatt
     5101 agagaaaaca taaatgaaaa aactcggatg gttttaattc aacgctctag aggttatagc
     5161 ttaaggccta gtcttagcat tgacgaaata gaacacctta tcaaatttat taaaagtatt
     5221 aaacatgaca tcatatgttt tgtagataat tgttatggtg aatttgttga ggcaagagaa
     5281 cctatagaag taggtgcgga tttgtgtgcc ggttccctca taaaaaatcc ggggggtggg
     5341 gttgcgccta cgggaggcta tgtggtaggt aaaagagact tagttaggca gtgcgcatat
     5401 aggctgacag caccggggct tgataaaagc atcggttcag cattgttttc caaccatcta
     5461 atagcccaag gactttttta ttcaccgaag gtagtgggtg aggctttaaa aggggctgta
     5521 tacacttctc gaatgatgga aaaactcggc tttgaggtaa gtccaaggtt cgatgaaaaa
     5581 cgatgtgata tcgtaactgc tgcaatattg ggagaagaag atgcattaat aagcttctgc
     5641 agaggtattc aaaaagcagg gccagtggat tctcatgttc aacctgaacc tagtgccatg
     5701 ccgggctatg aacataaagt gattatggca ggaggaagct tcatacaagg ctcttccatt
     5761 gagcttagcg ctgacggtcc actcagggaa ccatatgcgg tatacgtaca aggaggcctg
     5821 aattttgctc atgtaaaaat agggattatg agcggccttc aagctctaat ggatagcggc
     5881 aagctcacat tataaaaatt ttatcatcaa taaaaataaa agatgtgaca gagtagattt
     5941 ctgttacatc ttttcttgtc attcgaatac cacgggctat gcccgtggtt ccgaaaagct
     6001 tatagctatg
//
LOCUS       DAONUO010000103         5891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10066_length_5891_cov_2.846642, whole genome shotgun sequence.
ACCESSION   DAONUO010000103 DAONUO010000000
VERSION     DAONUO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_5891_cov_2.846642"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK768_04945"
     CDS             complement(<1..284)
                     /locus_tag="PK768_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60349.1"
                     /translation="MFMNINYLIDSSFFEDYKIAIVLGDEDMLFKSNIKSNIKYSVNI
                     EEYEYYDTLETRVIPAMPINYLIIYDFYLFLIDELESLEKILTEKYPIKD"
     gene            666..893
                     /locus_tag="PK768_04950"
     CDS             666..893
                     /locus_tag="PK768_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HQA60350.1"
                     /translation="MAVVATPLNSSLRIIVETGFDEEGKPIYRTRTYNNVKTAVSDED
                     LMSVANQLADIQSHPVYAFRRIVETQLTEEF"
     gene            947..1165
                     /locus_tag="PK768_04955"
     CDS             947..1165
                     /locus_tag="PK768_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HQA60351.1"
                     /translation="MDKSLELVFKNQAGKNARISVKDPKEDLTTQEVEALMNSIIEKN
                     VFETTGGDLVEIVGARLIQKEVVDLIIS"
     gene            1229..1396
                     /locus_tag="PK768_04960"
     CDS             1229..1396
                     /locus_tag="PK768_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HQA60352.1"
                     /translation="MMQDLVAQVANVGFPIAVSIYLLVRLEAKMESLTASIYELAKVI
                     EGIRDSAKTYS"
     gene            1464..1973
                     /locus_tag="PK768_04965"
     CDS             1464..1973
                     /locus_tag="PK768_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HQA60353.1"
                     /translation="MERLYPRFIVIHHTAGFDVPALTIDGYHAKQGFGVKITSPKPLV
                     EEYIRRGFRRTDGGVIVCIGYHYLIRTDGSVEKGRPDFIRGAHCVADNMNFKSIGVAL
                     TGNFCSKDNPRGEKGLTRPSKAQVTSLKELVHHLMEIYQIPRENVLGHKNVKNAATSC
                     PGDRFVFEL"
     gene            2042..2683
                     /locus_tag="PK768_04970"
     CDS             2042..2683
                     /locus_tag="PK768_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HQA60354.1"
                     /translation="MDLVQLVTEGIAAFFPVYDEGNVTHVITRSGKEFPIKRTCKTVL
                     KNLARFYNVDLAALREFYGKAVNKKQGVPIPMTTNLLLIPVKARKNPLGENDGTFGYV
                     NFKEIKELNDAGSGGCRIIFQNDMELEVHVSVATMKEYVKNAHIVEGIFLKRHFEGYK
                     GEDRALDKFEEANLPYEADSSPGDDDELFREYLLKLVLEIVHLIRRQKNQAAR"
     gene            complement(2716..3591)
                     /gene="dapA"
                     /locus_tag="PK768_04975"
     CDS             complement(2716..3591)
                     /gene="dapA"
                     /locus_tag="PK768_04975"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQA60355.1"
                     /translation="MNFGRVITAMVTPFDEDLKVDFKALEKLIEHLISTGTDTLLVTG
                     TTGESPTLSDEEKLELFRASKAIAGNRAKIMAGTGTNSTQHSIDLSVEAEKIGVDGLL
                     LVAPYYNKPTQAGLYKHFSMIAQAVDIPVVIYNVPGRTAVNVEPETLAKLSEIQNIVA
                     VKDAAGNLDITSKTRVLAPKLDIYSGDDSLTLPMLSVGGKGVISVASHVVGSEIKEMI
                     ESFEEEDTKKAEEIHLKLFPLFKTLFTITNPIPVKEALNMMGVKVGGFRPPMTPADEG
                     VKEAIKKALKDLGKI"
     gene            complement(3548..4771)
                     /locus_tag="PK768_04980"
     CDS             complement(3548..4771)
                     /locus_tag="PK768_04980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQA60356.1"
                     /translation="MQTSDVVIIGGGVIGCSIAYHLAKRGIPAHVIERDDIAMGSSGA
                     CDKAVLLQSKNPGIHLELALKSVKMFPELQKELDTDIEFLNHGGMIAIQNEKQWQVME
                     GFVERQRKVGLDVRLVDKDQARALQPAFSGDIIGSTYSPMDCEANPIYLSLGLYRGAK
                     KLGAKFSLRTEAKSIKLEKGRVKSAVTDSGEILCKYLVNATGAYAQFIGRMVGLKIPI
                     VPRRGQIIVTEPVAPLLRGDINCARYITAKFRPELLGTDEMAKLGVGLSLAQTVNGNI
                     LIGGTREFVGFNKGTTHRALKAILKHATSIVPALKDIHIIRSFSGLRPYTPDGLPILG
                     EVPEVPGFIMAAGHEGDGIALSAITGRIIADVIADGKPADDLNIDMSKLSLERFKEVD
                     VDEFWQSHNCYGYAL"
     gene            complement(4811..5074)
                     /locus_tag="PK768_04985"
     CDS             complement(4811..5074)
                     /locus_tag="PK768_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQA60357.1"
                     /translation="MNEEYICRCEEVSRKEIEEAIEDGARTIAGIKKRTRAGMGLCQG
                     RTCRRLVSQMLSKHTAPEDVLPDTSRSPVRAITVREIIDGEDR"
     gene            complement(5067..5585)
                     /locus_tag="PK768_04990"
     CDS             complement(5067..5585)
                     /locus_tag="PK768_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA60358.1"
                     /translation="MMEKKGVLYQGFPSMEELEKSPGVPSKDRIRKGPVACIECVQEI
                     PCNPCEEACPYGAIKVGKPITNLPVLDQDKCIGCGNCIAACPGLAIFVVNGAREKAQI
                     SFPYEYHPLPKRGDTVDCVDRSGKVVTSGRVLRVVKAPNYDHTAVITVEVDQAYIDVV
                     RSIKRLEGSDDE"
     gene            complement(5582..>5891)
                     /locus_tag="PK768_04995"
     CDS             complement(5582..>5891)
                     /locus_tag="PK768_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HQA60359.1"
                     /translation="EFTFIPPLGGHVPIHDENMETTLPGIYVAGDITGVEEASSAMEE
                     GRLAGVASAESLGYYDEETAKALKDEIWQRLNSLRTGPFGEGRFKAKIRQLQARRQLA
                     "
BASE COUNT         1407 a         1414 c         1435 g         1635 t
ORIGIN      
        1 atatctttaa ttggatattt ttctgtcaat attttttcta gggactctaa ttcatcaatc
       61 aggaacaaat agaaatcata aatgattaaa tagtttattg gcattgctgg aattactcta
      121 gtttctagag tatcataata ttcgtattct tctatgttta cactgtattt tatattactt
      181 ttaatattgc tcttaaacaa catgtcctca tcccctaaaa caatagcgat cttataatct
      241 tcaaagaaac tactatctat tagataatta atattcataa acatattacc ataattctcc
      301 ttttgtgtct atatctatca ttatgcctct tttaatatta tttatgcagt taaataggga
      361 ttatgtctat aaccgttcta taataagaca atagtgaggc agagtttatc aacttatggt
      421 tttgttgtga gttggttgtt ttttttggca atattggctc aggttggacc tagtgggctt
      481 tatttttaat ccgacttgtc caaaaaatgg tgagttaagc tgagtcgtct tcccggagga
      541 ggtcactcgg cttttttgtt tttcaggcag tctttttata atggaggcac aaacacagac
      601 ttcccctttc aggaggtgac cttttgaagt gggggaagcg aaatttgcaa aggaggtgaa
      661 agggcatggc agtagttgcg actccgctaa attccagtct tcgcataatc gtggaaaccg
      721 gcttcgatga agaaggcaaa cccatctaca ggaccaggac ttacaacaat gtgaaaaccg
      781 cagtttccga tgaagatttg atgagtgttg ccaatcaact ggctgacatt cagagccatc
      841 cggtgtatgc ctttcgccgg attgtcgaaa cccaactgac tgaagagttc tagagggaag
      901 gcccgcatcg ggctccctca gtttgatacg aagggaggtg aactgaatgg ataagagctt
      961 ggaactggtc tttaaaaacc aggccgggaa aaacgccagg atatccgtaa aggaccccaa
     1021 ggaggacctg actactcaag aggtagaggc cttgatgaac agcatcatag agaaaaacgt
     1081 gtttgagact actggcggtg acctggttga aatagtagga gcccgcctca ttcagaaaga
     1141 agttgtcgac ctcatcatca gttaaaactt ttagctgggc gtttaaggcg cccggctaaa
     1201 cttatatctt caattgggag ggaggattat gatgcaggac ctggtggctc aggtagccaa
     1261 tgtgggcttt cccattgccg tctccatcta ccttttggta agactggagg ccaagatgga
     1321 aagcctcact gccagcatct acgagctggc caaggttata gaaggtatac gggattcggc
     1381 taaaacttat tcatgattta aaaaagaggg atttgatgaa tattttgtac ctgtccctaa
     1441 aaattcataa aggagtggtt tttgtggaaa ggctttaccc aaggtttata gtaatccacc
     1501 atacggcggg ttttgatgtg cccgccttga ctatcgacgg ctatcatgcc aaacagggtt
     1561 tcggggtgaa aatcacatcg ccaaagccct tagtagagga atacatccgg cggggcttta
     1621 gaaggaccga tgggggcgtc atcgtctgca tagggtacca ctatcttatc aggactgacg
     1681 gcagcgtgga aaaggggcgg ccggatttta tccggggtgc ccactgcgtg gcagataaca
     1741 tgaactttaa gtctatcggc gtggccctaa cggggaactt ttgttccaag gacaaccctc
     1801 ggggtgaaaa ggggctgacg aggccgtcga aggctcaagt tacgtctctt aaagagctcg
     1861 tccatcacct gatggagatt tatcagatcc cgagggaaaa cgtattgggc cataaaaacg
     1921 taaaaaatgc ggcaacgagc tgccccggcg acagatttgt atttgaactt tagtgttttt
     1981 tgtttttttg acttgaccca aacatatgtt tggattataa tggttgtcgg aggtgcgtta
     2041 tatggatcta gtacaacttg tgacagaagg cattgcagct tttttccccg tgtatgacga
     2101 gggcaatgta acccatgtca taacgaggag cggcaaggag ttcccgataa aaaggacgtg
     2161 caaaactgta ttgaaaaacc tggccaggtt ctacaatgtg gacctggctg cgctcagaga
     2221 attctacggc aaagcggtca acaaaaagca aggagtgccc attccgatga caacaaacct
     2281 tctcctgata ccggtgaaag ctcgtaaaaa cccccttggg gaaaacgacg gcactttcgg
     2341 ctacgtaaac tttaaggaaa taaaggagct aaatgatgct ggcagcggcg gctgccgcat
     2401 tatctttcaa aatgatatgg agcttgaagt gcatgtaagt gtagccacca tgaaggagta
     2461 tgtgaaaaat gcgcatatag ttgaaggaat ttttctaaag cgccattttg aaggatataa
     2521 aggggaggat agagccttgg acaagttcga ggaagctaat ctgccctatg aagctgatag
     2581 ttccccggga gatgatgatg aactatttag ggaatacctc ttaaagctgg ttttggaaat
     2641 agttcatctg atccgcaggc aaaaaaatca ggccgcaagg taattgggcc tgtttttttt
     2701 gctttagatt atcacttaaa tcttgccaag gtcctttagg gcctttttga ttgcttcctt
     2761 tacaccttcg tcggcagggg tcatgggtgg cctgaagccg cctaccttca cgcccatcat
     2821 gtttagggct tcctttaccg gaatggggtt tgttatggta aagagggtct taaagagtgg
     2881 gaagagtttg aggtgaattt cctctgcctt tttggtatct tcctcttcaa agctctctat
     2941 catttcctta atttcgcttc ccaccacatg ggatgccacg ctgatgacac cttttcctcc
     3001 cacggacagc atgggcaggg taaggctgtc gtcaccgctg taaatatcaa gcttcggtgc
     3061 caggacacgg gttttgctgg ttatatccag gttgccggcg gcatccttta ccgccacgat
     3121 attttgtatt tctgacagtt tcgccagggt ctccggctcc acgttgacgg cggtcctgcc
     3181 gggcacattg tagataacca ccggtatgtc aacagcctgg gctatcatgg aaaagtgctt
     3241 gtacaaaccc gcctgggtgg gcttgttgta gtagggggct accaggagca ggccgtcaac
     3301 gccaattttc tcagcctcca ccgacagatc tatgctgtgc tgggtggagt tggtcccagt
     3361 ccctgccatg atctttgccc tattgccggc aattgctttc gaagccctga acagctccag
     3421 cttttcctca tcggaaagtg tcggcgattc gccggtggta cccgttacca ggagggtatc
     3481 ggtgccggtg cttatcaggt gctcgatgag cttttccagt gccttaaagt ccacctttaa
     3541 gtcctcgtca aagggcgtaa ccatagcagt tatgactctg ccaaaattca tctacatcta
     3601 cctccttgaa tctttctagg gaaagtttac tcatgtcgat atttaaatca tcggcaggtt
     3661 tgccgtcggc aattacatcg gcgatgatcc tccccgtaat tgcagatagg gctatgccgt
     3721 ctccttcatg gcccgctgcc ataatgaagc ctggaacttc tggcacttct ccaagtattg
     3781 gcagcccgtc gggggtgtaa ggccgaaggc ccgagaagct ccggatgatg tgaatgtctt
     3841 tcagggcggg cacgatggat gttgcatgtt ttaatatggc cttcaatgcc ctgtgagttg
     3901 tgcccttgtt aaagcccacg aattcccggg tgccgcctat taagatattg ccgtttacgg
     3961 tctgggcaag ggacaagcct acccctagct ttgccatttc atcggtgccc aaaagttcag
     4021 gcctgaattt ggccgtaatg tagcgggcac agtttatatc cccgcggagc agcggagcga
     4081 caggttccgt aactattatc tggccacgcc tcggcacgat gggaatcttg aggcctacca
     4141 tcctgccgat aaactgtgca taggccccgg tagcattgac caggtacttg cataagatct
     4201 cgccgctatc ggtcacggcg gattttaccc ggcctttctc tagtttaata cttttcgcct
     4261 cggttctcaa ggaaaacttg gctccgagtt tttttgcccc ccgatagagg cccaaactca
     4321 aataaatggg attcgcttca cagtccatgg gactgtatgt ggaaccgatg atgtccccgg
     4381 aaaaggccgg ttgaagggca cgggcctggt ctttatcgac aagccgtaca tcaaggccca
     4441 ctttcctctg ccgctccaca aaaccctcca tcacctgcca ctgtttttcg ttttgtattg
     4501 caatcatgcc accgtggttt aaaaactcta tatcagtatc tagctccttt tgcagctccg
     4561 gaaacatctt tacgctcttt aaggccagtt ccaggtgaat tcccgggttt ttagactgca
     4621 aaagcacggc cttatcacag gctcccgacg agcccatggc aatgtcgtcc ctttcgatta
     4681 catgggcggg aattcccctc ttggccaggt ggtaggctat cgagcagcct atgacgccac
     4741 cgcctatgat tacgacatcc gatgtttgca agagacctcc tcctctttag ctttcatttt
     4801 tattggaaaa ctatctatct tcaccgtcta tgatttccct aacggtaatg gcccttaccg
     4861 gggacctgga agtatcgggc aggacatctt caggagccgt atgttttgat agcatctggc
     4921 ttaccaggcg gcggcaggtc ctgccctggc aaaggcccat gccggcccgg gtcctcttct
     4981 ttatacctgc aattgttctg gctccgtctt ctatggcctc ttctatttcc tttctgctta
     5041 cttcctcaca gcggcagata tattcttcat tcatcatcag acccctccaa tcgctttatg
     5101 ctgcgcacga catctatata tgcctggtca acctctacgg taataactgc ggtatgatcg
     5161 taattcgggg cttttacgac tctcaaaacc ctgccggaag ttacgacctt tccgctgcgg
     5221 tcaacgcagt ccaccgtgtc gcctctcttc ggcaggggat ggtattcgta gggaaagctt
     5281 atctgggcct tttccctggc gccgtttacc acgaatatgg caaggccggg acaggcggcg
     5341 atgcaatttc cgcaacctat gcatttatcc tggtccagga ccggcagatt cgtgatgggc
     5401 tttcctactt ttattgctcc gtaggggcag gcctcttcac aggggttgca ggggatttcc
     5461 tggacgcact ctatacatgc cacgggcccc ttcctgatcc tgtccttgct aggaactccg
     5521 gggctctttt caagctcctc catcgaaggg aagccctggt acagcactcc ctttttttcc
     5581 atcatgccaa ctgcctcctt gcttgcaatt gccttatttt cgccttaaat ctgccttccc
     5641 caaagggacc ggtccgcagg gaattgaggc gctgccagat ttcgtccttt aaggcctttg
     5701 cggtttcttc atcgtagtag cccaggcttt cagcactggc cactccggcc agccttccct
     5761 cttccatggc ggaagaagct tcctccactc cggttatgtc accggccacg tagatgcctg
     5821 ggagggtagt ctccatgttt tcatcgtgaa tgggcacgtg gccgccaagg ggcgggataa
     5881 aggtaaactc g
//
LOCUS       DAONUO010000104         5848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10145_length_5848_cov_3.536164, whole genome shotgun sequence.
ACCESSION   DAONUO010000104 DAONUO010000000
VERSION     DAONUO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10145_length_5848_cov_3.536164"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK768_05000"
     CDS             complement(<1..438)
                     /locus_tag="PK768_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQA60360.1"
                     /translation="MILLWDLIIKNGRILTMDSSKELNKSLLIKDGLIADIVENSIAE
                     KLDACRVVDAEGLVVMPGIVDCHTHLCEYATQGVHQTVGKSQKMAAVSNFLDCLKSGI
                     TTLGEHILGHPVLSTTIEELKETASQTALTVKFATGTCCVGTEP"
     gene            complement(450..1457)
                     /locus_tag="PK768_05005"
     CDS             complement(450..1457)
                     /locus_tag="PK768_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQA60361.1"
                     /translation="MKVKDLIKVLDDITGGRVVKDISDIFTGKNPFVVIKSSNIPGKS
                     VMETPGLVYGDPEAEVKKVAVTMTLTEGNIELAGATGVNAIIAHHPIADAANSGGVTL
                     SYYLNLYNIAVFELHEAFHGLHPGISYIHGHKPFRVDIRYGGIPGNIVYVGKALDEIK
                     TLGDILERLNKMMDMEKEQKVMEVERTHFDCCDMKDSATALQGEILLGTPESPVNTIL
                     HIFPHTGFTPDHLVKVKEEHPEIDTVLATISRVKKDSDLVKKAEEMGLNFICGNSHAL
                     EIFENGLPLAYALRHYLPEDIEIVLFRERICSIPLKDFGSPNIKEYAKYITENYLINK
                     K"
     gene            complement(1900..2274)
                     /locus_tag="PK768_05010"
     CDS             complement(1900..2274)
                     /locus_tag="PK768_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HQA60362.1"
                     /translation="MKNVGDFKQEIIKINNRVNEEMYGRGLDWQKIDIIGDKIIILAL
                     NRRISILKHVDEKDAFTARLMDLALLNEFKVRFKKQFEDKFQLKIRTILKDYDPVNQL
                     AGTIIITVQPVEDFLKTLDKHS"
     gene            2445..2921
                     /locus_tag="PK768_05015"
     CDS             2445..2921
                     /locus_tag="PK768_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60363.1"
                     /translation="MQVGIELYAQRAVYIMDNHFMRLRVTNNGKLIEDSCSEDVFRFF
                     GSIIPGRRLAGVGRKSIYEPSFLLGRIFEADPSSPIDFLFIDPKDDIKLIIENNIWLD
                     PGVMLQEVMLKVFSDKKTLEIPLSRPDIKIDWSGRGNFAIDIGGFIRELNAARIRV"
     gene            3054..4205
                     /locus_tag="PK768_05020"
     CDS             3054..4205
                     /locus_tag="PK768_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQA60364.1"
                     /translation="MNIAILSTYYPRECGIASFSRDLRDNLMLWGEQVLIFAISDKSG
                     LYSYPEEVVFELEEENRDHYTIAAELANSSDIDLVFVEHEYGIFGGDDGGYVLDFVES
                     LKKPFILNTHTVLPEPDFHKRRILSKLGQKATAVICMTHRSAELLNKVYNIPHKKLFM
                     VHHGVPEFEEKPGEVIKKAYGIENRPLVVTFGFLGPGKGIELGIKAISYLKDKYPDII
                     YLVAGETHPNLKKKMGEAYRESLVELIQTLGVDDNIKFINKYLSLEELGEVLYMADVY
                     LTPYPHRHQAVSGTLSYAIGCGRAIVSTPYDYALEVLAEGRGLVASDADPEELAQLID
                     KIFSNPKLKERLEQRAGKLGKTMSWPQVGKRYVDIIGNIMQLHAKSEVF"
     gene            4207..5229
                     /locus_tag="PK768_05025"
     CDS             4207..5229
                     /locus_tag="PK768_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60365.1"
                     /translation="MYGHFKRMTDSTGIIQFSDKSRPLVESGYTVDDNARALIVAMGM
                     EERERERLIKTYTSFLQEAQNQDGTWQNLKVHDKYYTVINSEDSIGRGVLAASFAASC
                     SIAEIQKIARKMLRRVMPRAVQVNSPRAIAYVLMGVVKMIASLKCKYMIPQAKYLAYK
                     LLNLYELNRSTSWHWFEDRMTYCNAVLPHALFGFYAVSEDGKVLTAAKNTLNFLANSL
                     FQKGYLNIVGNRGWWLKKNQIPPYDQQPVDAASVVLACLQAYAVTAEREYIETAKLAY
                     DWYWGKNINNVPLYNEKTEGCHDALVPNGVNLNQGAEAVISFLMAHQVLHDIEAKRDE
                     LLIPAV"
     gene            5242..>5848
                     /locus_tag="PK768_05030"
     CDS             5242..>5848
                     /locus_tag="PK768_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HQA60366.1"
                     /translation="MGHNDMLFEETIKRVKKDLSTINDVDVVIGIPFYNEGDTLKEVI
                     SVAKESLESKKRKLIACVGDPAGKEALRLIRQHFTDELVSFLMPPGANGRGNSIRAIL
                     ELARFFQSDVVLLEADLVSQGNCGIKPDWIDRMAKPVFEDYDMSIASFVRHPFEDTIG
                     KILVAPLIAALYEMKFSDPLSGVFAISRDAVEEFCSEFDQNR"
BASE COUNT         1714 a         1135 c         1293 g         1706 t
ORIGIN      
        1 gggttcagtt cccacacagc aggtgccggt ggcaaatttt acggtcagtg ccgtttgact
       61 ggcagtttcc tttaattcct ctatagttgt tgatagaacc gggtgcccca aaatatgctc
      121 acctaatgta gtaatgccgc tttttaagca gtcaagaaaa ttagatactg cagccatttt
      181 ttgtgatttg ccgacagttt gatgaactcc ctgggtggca tattcgcaca agtgtgtatg
      241 gcaatctaca atgcccggca taactacaag accttcggca tctacgactc tacaagcatc
      301 tagcttttcg gcaatgctgt tttcaacgat atcagcgata agcccatcct taatcagtaa
      361 ggatttattc agctctttgg aagaatccat agtcagtata cggccgtttt tgataatcaa
      421 atcccacaaa agaatcactc cgattataat tatttcttat ttatcaaata gttttcagtg
      481 atatatttgg cgtattcttt aatattgggc gaaccaaaat ccttcaaggg tatagagcag
      541 attctctctc taaacaggac gatttctata tcttcaggca ggtaatgtct taaagcatat
      601 gctaaaggta gtccattttc aaagatctcc agtgcatggg aattgccgca aatgaagttc
      661 agccccattt cctcggcctt tttaaccaga tcgctgtctt tctttacccg gctaatggtc
      721 gccaaaacgg tatctatttc aggatgttct tccttaactt tcacgagatg gtcaggcgta
      781 aatccggtat gcgggaagat atggagtatg gtgtttaccg ggctttcagg cgttcctaag
      841 agaatttcgc cttgaagtgc tgttgccgaa tctttcatat cgcagcaatc aaaatgagtt
      901 ctttctactt ccattacttt ctgctctttt tccatgtcca tcattttgtt gagtcgttcc
      961 aatatatcgc caagggtttt aatctcgtct agagctttgc ctacatatac gatgtttccg
     1021 gggatgccgc cgtaccttat atccactctg aagggtttgt ggccgtgaat gtaggaaatg
     1081 ccgggatgca gaccgtggaa tgcttcgtga agttcaaaaa ctgctatgtt atatagattc
     1141 aaataataac ttaaggttac tccacctgaa ttagcagcat cggctatggg gtgatgggct
     1201 ataatagcat tgactcctgt ggcccctgcc agttcgatat tgccttcggt taaggtcatg
     1261 gttacggcaa cttttttaac ctcagcttcc gggtcaccgt agacgaggcc gggggtttcc
     1321 atgactgatt ttccgggaat gtttgaagac ttaattacga caaaaggatt tttgcccgta
     1381 aaaatatcac ttatgtcttt gacaaccctc cctcctgtaa tatcgtcaag taccttgatt
     1441 aaatctttta ccttcataaa aggctcctcc ttttttagga tatttgtgaa aagcaaaagg
     1501 cctgattaca tatagtttta tccttgaaac aattatatat gttgaggggc ggcgttaatt
     1561 tgagtacctg cgctcctcct catcaatgat gagctattta aataacactt cgacacaaaa
     1621 atcgaaaatc cttccattat tttgataagt tgtaaaattt tttttactgc atataatact
     1681 gtgtattata aatattttta tagttgtaca aataaataga ggccgccaaa taaatacctc
     1741 atcgagagaa aattacgcta ccattctcaa tactttaaga aaatagcata acagttctct
     1801 ttaaggtatt tatttgacgg cctcgaacta tccttgtgga tctttcttat ccagtcaagt
     1861 attaagtttt ttacaatact taatattaaa atacattatt tatgaatgct tgtcaagggt
     1921 ttttaaaaaa tcttctacag gttgcacagt tattattatt gtgcccgcta gctggttgac
     1981 cggatcgtaa tcttttagga tggttcgaat ttttaattga aacttatctt caaactgctt
     2041 tttaaatctg accttaaact catttagcaa tgccaggtcc atcaatctgg cagtaaaagc
     2101 atctttttca tcaacatgtt ttagtattga aattctgcga tttaatgcta aaatgataat
     2161 tttgtcgccg ataatgtcaa ttttctgcca atcaagacct ctcccgtaca tttcctcgtt
     2221 gaccctgttg ttaatcttaa tgatttcctg tttaaaatct ccgacattct tcaaggcatc
     2281 aactccttcg cacatactac tataaatata taatgatttc actgttaata ttctacaaaa
     2341 tcgggatttg tcctgcataa caagtgtaaa atcaatgtaa caagaagacc ggcctgtgaa
     2401 tatgatgaaa tgaacgggta attttattgg gaggttgatc atctatgcag gtgggaatcg
     2461 agctttatgc gcaaagggca gtttacatca tggataacca ttttatgcga cttagggtta
     2521 caaacaacgg caagctaata gaagacagct gttcggaaga tgtatttaga ttttttggaa
     2581 gcatcattcc tggaagaaga ttagctggag tggggagaaa gagtatttat gagccaagtt
     2641 ttttactcgg caggatattc gaggcggatc cttcatcacc catcgatttt ttgtttatcg
     2701 atccgaaaga tgacatcaaa ttgatcattg aaaataatat ttggctggat cctggtgtaa
     2761 tgcttcaaga agtcatgtta aaggtctttt cggataaaaa gaccctggaa attcccctaa
     2821 gcagacctga catcaaaatt gattggtctg gtcgaggtaa ctttgcaatt gatatcggtg
     2881 gatttattag ggagctgaat gctgcgagga taagagtatg agattttgcc ctggcaacac
     2941 agggcatttt ttgtgccaaa atcgaggatg gataatatag ggataaatta gcaataatat
     3001 taatgtatgt ggcaaagtgt ttcaaaaata ataattatgt aaggagtgtt caaatgaata
     3061 ttgctatatt atccacctac tacccccgtg aatgtgggat agcttcattt tcaagggatc
     3121 taagagataa ccttatgtta tggggagagc aggttttaat ttttgcaatc agcgataaaa
     3181 gcggtttgta cagttatccg gaagaagtgg tttttgaact tgaggaggaa aaccgggatc
     3241 actacaccat tgcagcggaa cttgccaatt cttcggatat agacctggtc tttgtcgagc
     3301 atgagtatgg gatattcggc ggtgatgacg gcggatacgt gctggacttt gtggaaagcc
     3361 tcaagaagcc ttttatattg aatacgcata ctgtgctgcc ggaaccggat tttcacaagc
     3421 gtaggatatt gtcaaaacta ggccaaaaag caacggcagt catatgtatg acacatagat
     3481 cggccgaact attaaataaa gtgtacaaca ttccccacaa aaagcttttc atggtccatc
     3541 acggggtgcc cgagtttgag gaaaaacccg gtgaagttat caaaaaagct tatggcatag
     3601 aaaacaggcc tctagtagta acctttggct ttttaggtcc cggcaagggg attgaactgg
     3661 gtataaaggc catatcttat ttaaaagata aataccccga cattatttac ctggtagcag
     3721 gggaaactca ccccaattta aagaagaaaa tgggggaagc ttacagggag tccctggtcg
     3781 agcttataca aaccctggga gttgatgaca acataaagtt cataaataaa tacctgagcc
     3841 tggaagaact aggtgaagtc ctttacatgg cggatgttta tctcactccg tacccccatc
     3901 gccatcaggc agtaagcggg acactatctt atgccatcgg ttgcggcagg gccatcgttt
     3961 ccacccccta tgactacgct ttggaggtcc tggcagaggg caggggcctt gtagcttcag
     4021 atgctgatcc tgaagagttg gcacagctta tagacaagat tttttctaat cccaagctta
     4081 aggaaaggtt ggagcaaagg gctggcaaat tgggtaagac catgtcatgg ccacaagttg
     4141 gaaaaagata tgtagatata atagggaata tcatgcagct acatgccaag agtgaggtgt
     4201 tttaaggtgt acggccattt caagcgcatg acggattcca ccggaattat ccagtttagc
     4261 gataaatcaa gaccccttgt tgaaagtggc tatacggtgg atgacaatgc ccgggcgctg
     4321 atcgttgcaa tgggcatgga agaacgagag cgggagagac tgattaagac atataccagc
     4381 tttttgcagg aagctcaaaa tcaggatggg acctggcaaa acctgaaggt ccatgacaag
     4441 tactatactg taatcaattc ggaggacagc atcggcagag gtgtgcttgc agcaagcttt
     4501 gctgctagtt gcagcatcgc agaaattcaa aagattgcaa ggaagatgtt aaggcgggta
     4561 atgccaagag ccgtgcaggt caactcccca agagccatag cctatgtgct gatgggggta
     4621 gtaaaaatga tagcatcctt aaaatgcaaa tatatgattc cgcaagccaa atatcttgcc
     4681 tataagcttt taaatcttta tgaactgaat aggagcacaa gctggcactg gtttgaagac
     4741 agaatgacct actgcaatgc ggtgctccct catgcactgt ttggttttta tgctgtaagt
     4801 gaagatggga aggtactgac agctgcaaaa aacaccttga attttcttgc caattccctt
     4861 tttcaaaagg gatatcttaa tattgtgggc aatcgaggat ggtggcttaa aaaaaaccag
     4921 atacctccgt acgaccagca gccggtagat gctgcatcag ttgtcctcgc ctgcctgcag
     4981 gcatatgcgg taacagctga gagggagtat atagaaactg caaagctcgc atatgactgg
     5041 tattggggga aaaatataaa taatgtcccc ctatacaacg aaaaaaccga aggttgccat
     5101 gacgcattgg ttcccaatgg ggtaaaccta aatcaaggtg cagaagctgt aatctccttt
     5161 ttgatggccc accaggtttt gcacgatatt gaagcaaaaa gagatgaatt attgattccc
     5221 gcagtatagg agggaccatt attgggtcat aatgatatgc tttttgaaga gaccataaaa
     5281 cgggtaaaaa aagacctgag cacaataaat gatgttgatg tagtgatagg tatccccttt
     5341 tacaatgaag gggatacctt aaaagaggtt atatccgttg caaaggaaag tctcgagtca
     5401 aaaaaacgca aacttatcgc atgcgtaggc gacccggcag gcaaagaggc tctaagactt
     5461 ataaggcaac acttcactga tgagctagtt agttttttga tgccgccagg tgctaacggg
     5521 cggggtaaca gtattagggc cattttagaa ctggcccgtt ttttccaatc tgatgtcgtc
     5581 cttttagaag cagacctggt gagtcagggc aactgtggaa taaagcctga ctggattgac
     5641 cggatggcaa aaccagtatt cgaagactat gacatgtcaa ttgcaagttt cgtgcggcat
     5701 ccctttgaag atactatcgg caagattctg gttgcacctc taattgcagc cctgtatgaa
     5761 atgaaattta gcgacccttt gagcggcgtt tttgcaatta gccgcgatgc agtggaagaa
     5821 ttttgctccg agtttgacca aaacaggg
//
LOCUS       DAONUO010000105         5840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10168_length_5840_cov_3.863267, whole genome shotgun sequence.
ACCESSION   DAONUO010000105 DAONUO010000000
VERSION     DAONUO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10168_length_5840_cov_3.863267"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK768_05035"
     CDS             complement(<1..387)
                     /locus_tag="PK768_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60367.1"
                     /translation="MGVLIIRILILALSLVFLFPVPVMASDNNDYITVYNRVHEAISE
                     LKEMLPEWHDNLKPGEFDCSESSAFLQGYLQYRGIECEIVVAYSKNYENCHAWVEVPY
                     NHGVIPLEPTVLVDDPFSNCWLRKCYP"
     gene            complement(528..719)
                     /locus_tag="PK768_05040"
     CDS             complement(528..719)
                     /locus_tag="PK768_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60368.1"
                     /translation="MAKFTMVPTDFGSKSKEKTLETQVKNAFPDNPDIEEIAALADEL
                     WCAGEDDIQEILEELEELE"
     gene            complement(731..1501)
                     /locus_tag="PK768_05045"
     CDS             complement(731..1501)
                     /locus_tag="PK768_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HQA60369.1"
                     /translation="MPSIVSIVQVGCGGTGGYLVPKIARLLMTLAEYRKELKVAYVLN
                     DYDFVEEQNLYRQNFIRSDMGKNKADVLAIRYGSHFGIDIRSCSEKLEVADQLKKLFL
                     VQRNDGIHWSPEILCVLIGAVDNNKARKVMHDLFQTWDGFGYGHPLIYIDAGNGKFTG
                     QVVIGYRTNQVILPPVGKVFPEALIEEPETQPVVNCALNALQNPQNIGANDIAATLVF
                     SVVNILLTNDEINSHIITFDGQTQEINTRKGRAQEERK"
     gene            complement(1537..1713)
                     /locus_tag="PK768_05050"
     CDS             complement(1537..1713)
                     /locus_tag="PK768_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60370.1"
                     /translation="MSTLELNSPEGNKAEYEAARNRIIADLAIEAYNKGQIRSEDDGF
                     LGVLARVIKLVKED"
     gene            complement(1730..2080)
                     /locus_tag="PK768_05055"
     CDS             complement(1730..2080)
                     /locus_tag="PK768_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60371.1"
                     /translation="MNLFGEEFEQPAENACGAAAAKAPKKGKKASTPAKKDAFEEKLP
                     QRNIQVKMYSDFYSYEAPAELAEPTLDDVRKWLINNHGFTELADPKRVGMFIVTPEGQ
                     DPYVYCGVKFEKMG"
     gene            complement(2152..2793)
                     /locus_tag="PK768_05060"
     CDS             complement(2152..2793)
                     /locus_tag="PK768_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60372.1"
                     /translation="MITIQLREDGAFVELEYNGIKSFRECDINSLIDLLNDAVRETKT
                     HEVIESPLLPANTIKFDRSAYENEYVLYMYREPVIDQIIFESREYNVGYPATIYRFSV
                     RNDFISRISVWAIKDKVIRPDIPVYHYPFYNVFDDGKVCIGANRIEIEAPWQLHKVPD
                     ILRRMPSNPGTRLSNDSGLEGDSLFRAIENKPFPVEWLKPTNKTLKMILRNEG"
     gene            complement(2947..3306)
                     /locus_tag="PK768_05065"
     CDS             complement(2947..3306)
                     /locus_tag="PK768_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60373.1"
                     /translation="MNYIEEMGYDEEIIDQYLGTLNLLQIMLDIKKELGSNADIAGRY
                     IGCCSCCDCTHLFLSNIITVALGLNDEQGSIITVDYWVYEDFIEKYPGIKILDDEGDE
                     MVEIIFPTVYADICEQV"
     gene            complement(3318..3641)
                     /locus_tag="PK768_05070"
     CDS             complement(3318..3641)
                     /locus_tag="PK768_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60374.1"
                     /translation="MKKLDLQYIKEHLPALVTRTVQTDHEVRKKAQRVLYLMRLLEDQ
                     IITEEILSVPREEWDIGDEIAALCDGSTPVAQYIFKTDETRRWYNRCYQNFRYESLPR
                     PAEFV"
     gene            complement(3787..4929)
                     /locus_tag="PK768_05075"
     CDS             complement(3787..4929)
                     /locus_tag="PK768_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60375.1"
                     /translation="MSAWENLTGVEGAQTGYLGKRFSYARKESKKGIRWDFIVPLMEE
                     IGLERYIPQRPVAADAFRRACQDIGKKYHYDADGKKYKVTVVMIDESADPIIRNIQVT
                     EIDRKNKTSTEGKLVAQIQFSRATEYVTAYYGYEAGYEFESCPEFVMDRISAAISRYL
                     KECSLVSEQQLHNIVQRILQDAGNPVNKIPSTWNIPITREELLNKLLILAERLNEYEK
                     DIFVTDTLPVVSSDAMVTEKFKNDAVVYAIERLNTLYAKAKDDISTARNVEKATERAT
                     AQLKAEGERVMSLIEEYETILGQAMDEVRQAKQLTEDKLKEFVESPEQQQKHREGMPK
                     RRIRSASQPVAETKPAVQSEAPAPVRKIRPMKAMEDTQNTQLALNF"
     gene            complement(5259..5510)
                     /locus_tag="PK768_05080"
     CDS             complement(5259..5510)
                     /locus_tag="PK768_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60376.1"
                     /translation="MAKIFVRPDGRIEGLYTDTVPLKDLGHLNVRRATHVEFCEKRQE
                     WVVTLPDGTEVHHDPSREKALEWERTYCEKKLLEGYRVI"
     gene            complement(5510..>5840)
                     /locus_tag="PK768_05085"
     CDS             complement(5510..>5840)
                     /locus_tag="PK768_05085"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HQA60377.1"
                     /translation="NEAKISKFLQAYNLAAVEELIQRQGLHYVASTLPDGSVVYETIS
                     PSDATDHRVKTTVDPAGNLKVEAEGFAGSSCTEATAFLKSLGIVEHNEYKPEYYAEEE
                     LLKEREF"
BASE COUNT         1435 a         1441 c         1115 g         1849 t
ORIGIN      
        1 aggataacat tttcgcaacc aacaatttga aaaaggatca tccactagga cagttggttc
       61 tagtggaatt accccatggt tgtaaggaac ttccacccaa gcgtgacagt tttcataatt
      121 tttgctgtaa gcaactacaa tctcgcactc tataccccga tactgcaaat acccctgcaa
      181 gaaagcagag gattccgagc agtcaaattc gccaggtttt agattatcat gccactcagg
      241 tagcatttct ttgagttctg aaatggcttc atgaacccga ttatacactg ttatataatc
      301 gttattgtca ctagccatta cagggacggg gaacaagaaa accaaactta aagccaatat
      361 aagtattctt atgattaaca ctcccattac ataaaaattc ccctgaacat aataagttca
      421 gggattttac catctgcagc aaacccccac ttcaaacggc ctcagctgtt aagtgggggt
      481 aatttgcgcg cgttcccctt gttaaaaggg attagtttga aaaagattta ttccagttcc
      541 tccaattcct ctaagatttc ctgaatatcg tcctcacccg cacaccataa ttcgtcggcc
      601 aaagccgcaa tttcctcgat gtcagggtta tcaggaaaag cattttttac ctgagtctct
      661 agggtttttt ctttgctttt tgagccgaaa tcggttggaa ccatcgtgaa cttggccatt
      721 attgacctcc ttatttgcgt tcttcttggg ctcgaccttt cctggtattg atctcctggg
      781 tctgcccatc aaaagtgatt atatggctgt ttatttcgtc gttggtcaac aatatgttga
      841 ctaccgaaaa taccagtgtt gccgcaatgt cattagcccc tatattttgc gggttttgca
      901 gcgcatttaa agcacagttg acaaccggct gtgtctcggg ttcttctatc aatgcttctg
      961 ggaaaacctt acctaccggc ggcagtataa cttggttagt ccgataacct ataaccacct
     1021 gcccggtaaa cttcccgttt ccggcatcaa tatagattag tggatgacca tatccaaaac
     1081 catcccaggt ctggaataag tcgtgcatca ccttccgcgc cttgttgttg tctaccgcgc
     1141 ctattaagac acacagtatt tcgggcgacc aatgaatgcc atcgttgcgc tgtactaaaa
     1201 atagcttttt aagctggtcg gcaacttcga gtttttctga acaactgcgt atgtctatcc
     1261 cgaagtggct gccgtacctt atggctaata catcggcctt atttttaccc atatcacttc
     1321 tgatgaagtt ttggcggtat agattttgtt cttcgacaaa atcatagtcg tttaaaacat
     1381 atgccacctt gagttctttt ctatactctg ccagggtcat taacagcctg gcgattttgg
     1441 ggactagata accccctgtt cctccacagc ctacttggac aatggagact attgatggca
     1501 tttcgataac caccagcaac acctgccttt tcagggctaa tcctctttga ccaattttat
     1561 aactctggcc aacaccccta aaaatccatc atcttccgac ctaatttgcc ctttgttgta
     1621 ggcttctatg gccaagtccg caattatcct gttcctagcg gcttcatact cggccttgtt
     1681 gccctcgggg gaattgagtt ctaatgtact cacgcatcta tcctccctat catcccattt
     1741 tttcgaattt caccccacaa tagacgtacg gatcctgccc ttcgggagta acgataaaca
     1801 tccctactct cttaggatca gccagctctg tgaacccgtg gttgttgatc aaccacttcc
     1861 gtacatcatc caaagtgggt tccgctaatt ctgccggcgc ctcataggaa taaaagtccg
     1921 aatacatctt aacctgtata ttcctttgag ggagcttttc ttcaaaagca tcttttttag
     1981 ctggtgtgct ggcttttttg cctttcttcg gtgctttagc agctgcagct ccacaagcgt
     2041 tttccgctgg ttgctcaaat tcttcgccaa ataaattcat ttcctcactc ctttccctgc
     2101 gtagatcgca ggattttttc tatttttcca ataaaaaaga ggggttttga tttacccctc
     2161 gtttcttaaa atcattttta gcgttttgtt tgtgggcttt agccattcaa caggaaatgg
     2221 cttgttttca atagccctaa acaacgaatc accttccaac cctgagtcgt tgcttaatct
     2281 cgtccccggg ttagagggca tccttcgcaa aatatccggc accttatgca gttgccaggg
     2341 tgcttcaatc tctatgcggt tcgcccctat acaaactttg ccgtcgtcaa aaacattata
     2401 aaaaggataa tgataaactg gtatgtccgg ccttatcacc ttatccttaa tggcccagac
     2461 gctgatcctt gatataaagt catttctgac agagaaacgg tagattgttg cagggtatcc
     2521 cacgttgtat tctcgacttt caaatatgat ctggtcgatt accggctctc tatacatata
     2581 taagacatac tcgttttcat aagcactcct gtcaaattta attgtgttgg ccggtaacaa
     2641 cggggattct attacttcat gtgtttttgt ctctcttact gcatcattga gcaggtcaat
     2701 aagagagttt atatcacatt ccctaaatga ctttatcccg ttatattcca attccacgaa
     2761 tgccccgtct tccctgagtt gtatggttat catgacgcat ccctccccag ggcgattttt
     2821 ctgactttca taaagaaatc agaaaaatct tcaaattcag gccatttgaa gcattggaaa
     2881 tatatgtggc tgatattatc atctatcttc ctgtaggaaa aatttttttc tttgtcagga
     2941 gtagagttat acctgctcac atatatccgc atatacggta gggaaaatga tctcgaccat
     3001 ttcgtctccc tcatcatcga gtatctttat gccagggtat ttctctataa aatcttcata
     3061 tacccagtag tcaactgtaa taattgaccc ctgttcatcg tttaacccca atgctacagt
     3121 aataatattg cttagaaaca agtgcgtaca atcacagcac gaacagcatc ctatgtacct
     3181 tcctgcaata tccgcattgc tgcctaattc cttttttata tctagcataa tttgcagaag
     3241 atttagggta ccaagatact gatcaataat ttcttcgtca tatcccattt cttcgatata
     3301 attcacctgc ccacccccta aacaaattcc gccggtctag gaagtgactc ataccgaaaa
     3361 ttttggtaac agcgattata ccaacgcctt gtttcatcgg ttttaaaaat atactgtgcc
     3421 accggagttg acccatcaca caaggctgct atttcatccc ctatgtccca ttcttcgcgc
     3481 ggaacagaga gtatttcttc ggtgataatt tgatcctcaa gaagcctcat caggtataat
     3541 accctttgag ctttttttct gacctcatgg tctgtctgaa cagtccgggt gacgagtgct
     3601 ggcaggtgct ctttgatata ttgcaagtcc agctttttca tgactcactt ccttcccggc
     3661 gttaatcgcc agatttttga cgtaaaaacg attttggact taaccctcct tccttaaatt
     3721 gagatatgat tacaaaaact gcctcactct ttttaagagc agggcagtta taaaccgtat
     3781 tttactttag aaatttaaag ctaattgggt gttctgggta tcttccatag ccttcatagg
     3841 cctgattttt cgcacgggag caggagcttc actctggacg gctggctttg tctctgccac
     3901 aggctgtgac gcactcctga tcctgcgctt tggcataccc tcgcggtgtt tctgctgctg
     3961 ttcgggcgat tcgacaaact ctttgagctt gtcctcggtg agttgtttgg cctggcgcac
     4021 ttcgtccatt gcctggccca gtatagtttc gtattcctca atcaaggaca taaccctttc
     4081 accctcagct ttcaactggg cagtggcccg ttctgtggcc ttttctacat tgcgggctgt
     4141 agagatgtcg tcttttgcct ttgcataaag agtatttaat ctctctatag catagacgac
     4201 ggcatcattt ttgaattttt cagttaccat cgcatcactg gatactaccg gcagggtgtc
     4261 ggttacgaat atatcttttt catattcatt cagcctttcg gctaaaatca atagcttatt
     4321 aagtagttct tccctggtta tcggtatatt ccaggtgcta ggtatcttat tcacggggtt
     4381 tccggcatct tgcaaaattc tctgcacgat gttgtgtaac tgctgctcag ataccaggct
     4441 gcattctttt aggtatctgc taatagcagc cgatatcctg tccatcacaa actcgggaca
     4501 agattcaaac tcgtacccgg cttcataccc gtaatatgct gtcacatatt cggtagcccg
     4561 ggaaaactgg atctgtgcca ccaacttacc ctcggtagaa gtcttattct tccggtcgat
     4621 ttcggtaact tgaatattgc ggattatagg atcggcagat tcgtcgatca tgaccactgt
     4681 aaccttgtat tttttgccat cagcgtcata atggtatttc ttacctatat cctgacaagc
     4741 ccgtctaaat gcgtctgcgg caactggccg ttgtgggata tacctttcta gtcctatctc
     4801 ctccattaag ggcactataa aatcccaacg gatgcctttt ttactttctt tccgggcata
     4861 agaaaatctc ttgcctaaat accctgtctg ggcaccttca acgccagtca ggttttccca
     4921 tgctgacatg gatatacctc ctttcgcccg gctttaagcc gggcatttga tttccagctt
     4981 atagccgaca aaaggttgat tctgcgattg ttttttggtt atattaagta taggaatcgt
     5041 gggagttgtg gttctcccga caacgatcct agcgaatata tcgcgtagag agctgctacc
     5101 cactattaat tagtgtttag gcagctctat ttcgttatga gagctaacac gaacaatacc
     5161 aaaacaatgc aaagcatgat ttcgtattga ttcatgcaaa ccaactccct tctgctagaa
     5221 cgcagtcggg agcctccccg cgattccaaa agggattttt agattactcg gtatccctca
     5281 agtagctttt tctcgcagta tgtccgttcc cactctaaag ccttttctcg gctgggatca
     5341 tggtgtactt ctgtcccatc aggcagcgtc acaacccatt cctggcgttt ttcgcaaaac
     5401 tccacatgag ttgcacgcct tacatttaag tggcccaagt cttttaaagg tactgtatct
     5461 gtatacagcc cttctattcg accatcaggc ctgacgaaaa ttttagccat caaaactccc
     5521 tctctttcaa gagttcttcc tcggcgtagt attcgggctt atattcgtta tgttctacta
     5581 tgccaagact ctttaggaaa gcggtggcct cggtacaaga tgaacctgca aagccttcgg
     5641 cctccacctt taagttacca gccgggtcaa cagtagtttt tacccgatga tccgtcgcgt
     5701 cagaggggct tatggtttcg taaaccaccg aaccatccgg tagggtgctg gcaacatagt
     5761 gtaaaccctg cctttggatc agttcctcta ctgcggccag gttataggct tgtaggaatt
     5821 tacttatttt ggcttcattt
//
LOCUS       DAONUO010000106         5826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10193_length_5826_cov_3.716340, whole genome shotgun sequence.
ACCESSION   DAONUO010000106 DAONUO010000000
VERSION     DAONUO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10193_length_5826_cov_3.716340"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..2388)
                     /locus_tag="PK768_05090"
     CDS             complement(145..2388)
                     /locus_tag="PK768_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60378.1"
                     /translation="MVLNKRLMRILKENKLRYLGILVLIILGSYTFIVAAGLSQNLAV
                     LVTTFTEDHVQEDLSFSTDKPISDVSKLENTFDALIEEYMSFDAELSDKLTLRLLSET
                     KKVNIPAIIEGRALSGPGEILLDPAFAEANGYPIGSQIKAEGKTFTVVGFVSLPHYIY
                     PLKNVNDIIYSPKEFGVGVINREEFSDIVNSANVYSVKFNDRTRSLNRQAVELREHLR
                     AKGLTISNWIVIMNNKRARIVWASITGMKTMSVPVPAAMFLLSSLIIGIMFWRIIRRE
                     SVIIGTLYAQGYRRRELMRHYMAIPLVLAFAGGMIGSLLALPSIEPMVMAMISYYNVP
                     VPGIELSFLNVMIGILTPMIFLGLSGYMVIRSELKKSPAELMKGDKQKAKVNALERAF
                     KLERFKFSTKFWLREQFRSISRLVFLLLGVTSASVLMLFGFTVLNSMNHVFKTSDVYL
                     FEYEYTFRDLQYGEAPDGAETFNAGKFYPENNEEIEFYITGIEPDSTLVILKDNKGNV
                     IPNNQTNITKSLANRLGIKAGDTVTFINKEDGNHYTFHIDAVADSYMEQFIYVPIDEF
                     NKKMGLPEDSYIGLFSTKKLDIPDEKLSGTKSMSELPEAMDEFMGQMISMVALMTIVS
                     SIVALIILYLVTSLAIEENRNAISLFKVFGYRRREIRSLILNSSTFVVVAGFLIGIPV
                     AVASMGSLYGYLGDMINLVLPPIVSPLYVIISFAAIMLTYQLSKWMCAKNIDTVSMSE
                     ALKAGTE"
     gene            complement(2388..3086)
                     /locus_tag="PK768_05095"
     CDS             complement(2388..3086)
                     /locus_tag="PK768_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60379.1"
                     /translation="MFIDVSDLRKTYTNGMIKNEVLKGIGMKLGEGEIGVILGPSGSG
                     KSTLMNIIGGVDRGDSGKVIVDGIEVSKLNDDQLTEYRRDAIGFVFQFYNLVPNLTVG
                     ENIEVVSNISKSPLNIDEVLAEVQMHDKKHRFPRELSGGEQQRVSIARAIVKNPKLLL
                     CDEPTGALDFQTSRSILKLLQQVNRNFGTTILMITHNTAISAMANRVFKLRSGEIVDE
                     WVNQAPIPAEGIEW"
     gene            complement(3148..3780)
                     /locus_tag="PK768_05100"
     CDS             complement(3148..3780)
                     /locus_tag="PK768_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQA60380.1"
                     /translation="MTIIYSKFHSLETEKQERIINAALKEFARNGYDKASTNEIVKEA
                     EISKGSLFNYFNNKKELYLFLLDYVANIIEQIYAEVDWNEPDFFERIRQLGLIKFKVY
                     KKFPHAFNFLKALTHEDASEVKPAINKLKKDIVTSGLELGYQNIDWTKFRDDFNREKM
                     INIINWTMLSFSEENLSKLDSIDDVGMEVLSESDEYFEILKRCFYKKGIK"
     gene            complement(3999..4526)
                     /locus_tag="PK768_05105"
     CDS             complement(3999..4526)
                     /locus_tag="PK768_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HQA60381.1"
                     /translation="MACWQDLLSGRDVKVKYIPARKENYAKYKELPPFPSVIRGRFNP
                     FEWMKDPNRRMDYYDSTAPINAVDSEMLKGFAGAAGKVEGIVRILNAPEEGEKLQPGE
                     ILVATTTNVGWTPLFPKAAAIITDIGAPLSHAAIVARELGIPAVVGCGNATLRLKTGD
                     KVMVDGGQGIVHILS"
     gene            complement(4592..5272)
                     /locus_tag="PK768_05110"
     CDS             complement(4592..5272)
                     /locus_tag="PK768_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQA60382.1"
                     /translation="MKKMKEQFGGFQYSCGGQIMPLQRYDKEKILDACLTVFASHGYA
                     NTSTSMLAEAAGISKALIFHHFKSKKELYLSILDRCFEKGRIEMGFDALFENDFFAAK
                     EKSSIIKFHYYKKNPDLMKIIIEAFYATPDELKPDIKDKYGKLLVENEKQWKRLFEKV
                     PLREGVDREQAFRLVMITLNYFDEKYLSDVVNKDELDEKYLQDFLEERNSFLSMIRFG
                     IEKQERKH"
     gene            complement(5232..>5826)
                     /locus_tag="PK768_05115"
     CDS             complement(5232..>5826)
                     /locus_tag="PK768_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQA60383.1"
                     /translation="KAGFIGELIILFATSKSLSTCWYGHYKLSELERLMPHLQSPEQI
                     RESNMGYGYAKGVTTGRRAICITPLGYYESGGLRLMDRFQKKAISFKRKGIKELLTNP
                     DDYDKLSDDVMYALDLGRKAPSAANSQMWRFAFDDSYKTIIVAMPEGYRHFKWEHPNV
                     DIGICACHVWLGLIDKGYTPKVTVHEEDERAIWRISV"
BASE COUNT         1676 a         1308 c          980 g         1862 t
ORIGIN      
        1 ttattattgg tagggtccag gccttttttt cttggttttt actgtaaaag tcaggattta
       61 atcacaaaga accgcccctc tctgatgtat cagatatcgc cagcatggct acttcacatt
      121 tattccagcc tccacacagt ctcatcattc cgttccagct tttagcgcct cactcatgga
      181 aactgtatct atgttctttg cacacatcca cttgctgagc tgataagtca gcatgatagc
      241 tgcgaaggat attatcacat aaagggggct aacaataggt ggcagcacta ggtttatcat
      301 atctcctaga taaccataca atgaacccat tgatgccacc gcaacaggaa taccgattaa
      361 aaatccggcc acgacgacaa aggtggagct gtttaatatc agagaccgga tttcccgacg
      421 tcggtaaccg aacaccttga acagtgatat ggcgtttcta ttttcctcga tagccaacga
      481 agttacaaga tataagataa ttaatgccac aatgctggag acaatagtca tcaatgctac
      541 catggaaatc atctgaccca tgaattcatc catagcctca ggcaactcac tcatggattt
      601 tgttcctgac agcttttcgt caggaatgtc aagctttttt gtgctaaaga gcccaatata
      661 gctatcctcc ggcagcccca tttttttgtt aaactcatct attggcacat atataaactg
      721 ctccatgtag gaatcagcaa ctgcatcaat atgaaatgtg taatggttcc catcctcttt
      781 gtttataaag gttacagtgt ccccagcctt aatgcccaat cgatttgcca aagacttggt
      841 aatattagtt tgattgtttg gaatgacgtt ccccttatta tccttaagaa tcacaagggt
      901 tgaatcaggc tctatgccgg taatataaaa ctcaatttcc tcattgtttt caggataaaa
      961 cttccctgca ttaaaggtct ctgccccatc cggtgcttca ccgtattgca aatccctgaa
     1021 ggtgtattcg tattcaaaca ggtatacatc tgacgtttta aatacatggt tcatagaatt
     1081 taaaacagtg aaaccaaaga gcattaagac agaagcacta gtcactccca agagcaagaa
     1141 aacaagacgt gaaatgcttc taaactgctc acgcagccag aattttgtgc taaacttaaa
     1201 tctttcaagt ttaaaggccc tctccagggc attaaccttg gctttttgct tatcaccctt
     1261 cataagctcc gccggagact ttttaagctc cgaacgtata accatataac cgctgaggcc
     1321 taaaaatatc ataggtgtca gtattccgat cattacattt agaaagctta attcaatgcc
     1381 aggaacagga acattataat aggaaatcat ggccataacc attggctcta tcgatgggag
     1441 tgccaacaga cttcctatca ttccacctgc aaaagccagc accagtggaa tagccatata
     1501 gtgcctcatt aactctcgcc gccggtaacc ttgggcatag agtgtcccta taatcaccga
     1561 ttcccggcgg attatgcgcc agaacatgat tccgataatt aaggaagaca aaagaaacat
     1621 agcggctggt actggtacac tcatggtttt catacctgta atagatgccc aaactattct
     1681 tgcccgtttg ttgttcataa tgacaatcca gttggatatt gtcaaccctt ttgcccgtaa
     1741 atgttcacgc aattctaccg cctgtctgtt aaggctccgg gttctgtcat taaacttgac
     1801 agaatagacg ttggcagaat taactatatc cgaaaattcc tcacggttta taacacccac
     1861 tccaaattcc ttaggagaat atattatgtc attcacgttc tttagcggat agatatagtg
     1921 gggtaaggat acaaagccta ccacagtgaa tgttttacct tcagctttta tttggctccc
     1981 tattggataa ccgttggcct cggcaaaggc aggatcaagg agaatttctc ccggccccga
     2041 aagggcccgc ccttctatga tagcgggaat attcaccttt tttgtttcac ttaggagcct
     2101 tagtgtaagt ttatcggaca gttcagcatc aaagctcatg tattcctcaa tgagggcatc
     2161 aaatgtattc tctaattttg agacatcgga aataggcttg tctgttgaaa aggacagatc
     2221 ttcctgcaca tgatcctctg taaaagttgt gacaagcaca gcaagattct gagaaagccc
     2281 ggcagccaca ataaaagtat agcttccaag gataatgagt acaagaatgc caagatagcg
     2341 caatttgttc tctttcaaaa tccgcattag tctcttgttt aaaaccatta ccactcaatc
     2401 ccttccgccg gaataggggc ctgatttacc cattcatcta cgatttctcc gcttctaagc
     2461 ttaaataccc tattagccat agctgaaatg gcagtattat gggtaatcat caaaatggtt
     2521 gttccgaaat ttctgttcac ctgctgcaaa agtttcagga tactgcggga cgtctggaaa
     2581 tccaaagctc cagttggctc atcacagagc aatagctttg gatttttaac aatggctctg
     2641 gcaatagaga ccctttgctg ttcaccgccg ctcagttccc tgggaaaacg atgttttttg
     2701 tcatgcattt gcacctctgc taaaacttca tcgatgttca aaggtgattt gctgatattg
     2761 gaaacaactt caatattttc tccaactgta aggttaggaa ctaagttata gaactgaaat
     2821 acaaatccta tggcatcacg tctatattcg gttagttggt catcgttcaa tttgcttact
     2881 tcaattccat ctacaataac tttaccgcta tcgccgcgat caacaccccc gataatattc
     2941 attagagtgg atttccctga acctgatggt cctagaatta caccaatttc accttctcct
     3001 aatttcattc cgataccctt caaaacttca tttttaatca tcccatttgt gtaggtcttc
     3061 ctcaaatcgg aaacatcaat aaacaagtta ttcttccccc ttttttaacg agtaggagct
     3121 tatattacgc ctaattgacc taaaccttca ttttatgcct ttcttataaa aacaccgctt
     3181 taaaatttca aaatactcgt ctgattcact aaggacttcc atgccaacat catcaataga
     3241 atcaagctta ctcaaatttt cctcggaaaa actcaacatg gtccaattaa taatattaat
     3301 cattttttca cgattaaagt cgtcccgaaa ttttgtccaa tcgatgtttt gataccctag
     3361 ctccaatcca ctcgtaacaa tgtccttttt aagtttatta attgcaggtt taacttcaga
     3421 tgcatcctca tgggttaaag ccttaagaaa attaaaggca tgcggaaact ttttgtaaac
     3481 cttaaattta atcaatccca gttgtctgat tctttcaaag aaatctggct cattccaatc
     3541 cacttcagcg tagatctgct caataatatt agctacataa tcaagcagaa ataaatacag
     3601 ttccttttta ttgttaaaat agttaaatag cgagcctttt gatatttccg cttcctttac
     3661 aatttcgttt gtagaagcct tgtcgtaacc gtttcttgca aattctttta aagctgcatt
     3721 aataatgcgt tcttgctttt cagtttcaag actatgaaat tttgaatata taattgtcac
     3781 cccatttctt aagtattcga taaatttttg accacatcgg tcatttatat tatatattaa
     3841 aatgaccgat gtggtcaatg tggttttttg tataatatta tctttgcagc tgtttacccc
     3901 caaatattgc gagggcatgc gattttattt ttttttgtca aattaattct gcaaggtata
     3961 tctttattgg atagattatt ttttaacccg taaatctgtt aagacaatat atgcacaatc
     4021 ccctgtcctc cgtccaccat aaccttatct cccgtcttaa gtcttaaggt ggcgttaccg
     4081 catcctacaa ctgctggaat cccaagttct cttgcaacta tagcagcatg ggacaagggg
     4141 gcacctatgt cagttattat tgctgcagct ttaggaaata gaggagtcca acctacattg
     4201 gtggttgtag caactaaaat ttccccgggt tgcagctttt caccttcttc aggggcgtta
     4261 agaatacgca ctattccttc tacttttccc gctgcccctg caaagccctt tagcatttcc
     4321 gaatccactg catttattgg tgctgttgaa tcataataat ccattcttct attggggtct
     4381 ttcatccatt caaaaggatt aaaacgtcct ctaataactg acgggaaggg cggcagctct
     4441 ttatatttag catagttttc ttttcttgcg ggaatatact ttactttcac atctctacca
     4501 gataacagat cttgccaaca tgccaccttt gccgcctgcg gcagcctgaa gctctggagt
     4561 caattcgtga aaatttttca ccatattcat ctcagtgctt cctttcttgt ttttcaatac
     4621 caaatcgaat cattgaaaga aaactattcc tctcttcaag aaaatcttgg aggtactttt
     4681 cgtccaattc atccttatta actacatcgg ataaatattt ctcatcaaaa taatttaatg
     4741 taatcatgac caatctaaaa gcctgttccc tgtccacgcc ttctctgaga ggtacctttt
     4801 caaataagcg tttccattgt ttctcatttt cgacaagtaa ctttccatac ttatctttaa
     4861 tatctggttt tagttcatca ggcgtggcgt aaaaagcttc tataataatt ttcatcaaat
     4921 cgggattttt tttatagtaa tggaatttga tgatactgga tttttccttt gcagcaaaaa
     4981 aatcgttttc aaatagggca tcaaaaccca tttcgattct tcctttttca aaacatctgt
     5041 ccaaaatgct aagatacaat tctttcttgc tcttaaaatg atgaaatatc agtgctttgg
     5101 atataccagc ggcttccgct aacatcgaag tagaggtatt agcatatcca tgactagcaa
     5161 acactgtcag acaagcatct aaaatttttt ctttatcata acgttgtaaa ggcatgattt
     5221 gacctccaca gctatactga aatcctccaa attgctcttt catcttcttc atgaacagtc
     5281 actttcgggg tatatccttt gtcaattaat cccagccaga catgacaagc gcatatgcca
     5341 atatcgacgt ttgggtgttc ccatttaaaa tggcgatatc cttcgggcat agcgacaata
     5401 attgttttat aagaatcgtc aaaggcaaat ctccacattt gcgagtttgc agctgatggt
     5461 gcttttcttc ccaaatctag agcatacata acatcatcag aaagcttgtc ataatcatcg
     5521 ggatttgtaa gcaactcttt aattcctttt cgtttaaaac ttatcgcctt tttttgaaat
     5581 ctgtccatca gccgcaatcc accgctttcg taatagccta gtggtgttat acagattgct
     5641 cgtcgaccag tggttacccc ttttgcatat ccataaccca tatttgattc tctaatttgt
     5701 tcaggtgatt gtaaatgagg catgagtctc tccaactcag atagtttata atgtccgtac
     5761 caacatgttg aaagactttt acttgtggca aaaaggataa tcagctctcc aataaaaccg
     5821 gctttg
//
LOCUS       DAONUO010000107         5800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10241_length_5800_cov_3.712968, whole genome shotgun sequence.
ACCESSION   DAONUO010000107 DAONUO010000000
VERSION     DAONUO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10241_length_5800_cov_3.712968"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..2994)
                     /locus_tag="PK768_05120"
     CDS             complement(22..2994)
                     /locus_tag="PK768_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQA60384.1"
                     /translation="MNMRKIKGNSIIVRLLLSMLLVILVQTSLFAGNIWHGGTIRELN
                     NNSVDLLRQTVLSRKDYLENEMIQRWSNLSNFEQDIQSAMNSYLEEKDIELSTLKKEP
                     ALAFEFLERTIGDTIFTLRQRMVTGAFIVLATESGNEYPGVYLRDSDPLFNPADNSDL
                     SMEVGPSAVARKADIALGMGWMPSFPLLEDDASADFYFKPLNAAANNRSSRISDLGYW
                     GRPFKPNIYSAEAITYSIPLVDSDGNPYGVIGIDLTQDFLRKLLNYDEIADNKQGAYL
                     LAKNTGEDMAFENIVSSGPIFKKIFGDDTEVAIDGSTQYEDIYTISNTEASKNAVYGC
                     IHYLDIYDSNTPFEGDRWALVGIVEEQTLFKPARQIRLYVTISVVFSFIAGMLGAVLA
                     GMWFVKPIIKLVDDLRSSNPEKSVVLPKTNIAEIDDLASSIESLSSKVAESGDKLSRI
                     IGMMKIPIGAFEHDSKEDMVFCTSAFFDLVGIENKNKEARYISSTHFYEVLEGLKKRP
                     EPDMEDVYRHERGKGVIKWLRINIQEHGGKVLGVIEDVSREVLEKRRIEYDRDHDSLT
                     HLLNRRAFEAKVTRILRKENVKIAAFVMWDLDNLKYINDTYGHDFGDQYIKDAAKVLS
                     ELTIYNGVVARMSGDEFYAFIYGYDDKRQINDLVEMIQKKLYNTTIRMPNGGDLRIKA
                     SVGIAWYPEDSKNYYELMKYSDFAMYEVKSEDKGTIGEFDRKSYESKYFLLHGNEELD
                     NFIENELVDFVFEPIVDAREGKVFAYEALMRPKVPTLTSPEDIIKLAAAQSKLYHIER
                     ITWFKAMEAFQRHRGAFDDARLFINSVANQVLTDKDLRRFEREYKRDLHRIVIEVTEN
                     EQANEETIRKKQSIAKRWNIHLALDDFGAGYNSEIILLALSPSFVKVEKTLIRGVDRD
                     KNRQKLLETIISYSKDRKIKTIAEGIENKAEMDILIEFGVDYLQGYYIGKASHIPQKL
                     SPQVAREIKERNSLINRR"
     gene            complement(3013..4491)
                     /locus_tag="PK768_05125"
     CDS             complement(3013..4491)
                     /locus_tag="PK768_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQA60385.1"
                     /translation="MKGVKKCRRLISLLILCMCISFSLTACSKADKDGLDPENPISIE
                     IWHHYNGLQKTAFDALVMEFNETLGAEKGIIVEAFSQGNVDALQRKVMESVTKKIGAE
                     PMPDIFAAYADNAYQIDQLGHVADISEYLSPEEIDEYVDSYIREGRFGDGNGIKIFPT
                     AKATEVLMLNKTDWDKFANATGASLEELRTWEGLAKAAEKYYEWTDSLTAVPNDGKAF
                     FGRDAMANYIIVGSKQLGQEIFSVESGQVSIHVDEGIMRRLWDNFYIPYISGYYTAVG
                     RFRSDDAKTGEIIALVCSTSAIPYFPDTVIIDDREEYPIESLVLPLPNFENTAPFAVQ
                     QGAGMVVVKSDKLRERAAVEFLKWFTDIDRNIRFSLESGYLPVKKEANDIRVIQKFLA
                     DAKDNRVTEKMKSSLPVLMEQLESYELYTNDPFENGTNAREVLSKALIDKSKADREKV
                     IELLEKGNTREEAVKEVATEENFQEWLRGLRESLAAAINGGS"
     gene            complement(4680..5588)
                     /locus_tag="PK768_05130"
     CDS             complement(4680..5588)
                     /locus_tag="PK768_05130"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HQA60386.1"
                     /translation="MSKYRIAVVGFGNVGREVFHAIEESPDMETAGIVIRNPKKIQAT
                     KKIVGDVPVTTDVRELGKVDVAVLSIPSRSIAEVAPRFLEMGINTVDSFDIHGDDVIS
                     LREKLDEAAKAHDSVAVISAGWDPGSDSIVRALVEVVAPRGLTYTNFGPGMSLGHTVV
                     VKAIEGVEDALSMTIPQGHGLHKRLVYVQVKEGYDFDKIAEAVKTDPYFIHDETHVYK
                     VDDVKSLIDKGHGVHIQRKGVSGTTHNQRIEFIMSICNPAAAGQIMVAAARASLRQRP
                     GCYTLLEIPIIDFLHGDRVQLLHRLI"
     regulatory      complement(5673..>5800)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         1411 a         1487 c         1131 g         1771 t
ORIGIN      
        1 ctccctcatt ttaaaatccc atcatcgcct gttgattaaa gagttcctct cttttatttc
       61 cctcgcaact tggggcgaga gcttttgagg tatgtggctg gcttttccta tgtagtaacc
      121 ttgcaaataa tccacaccga attcaatcaa tatatccatc tctgctttat tctcaatacc
      181 ttctgcaata gttttaatct tcctgtcctt ggaatacgat attatggttt ccaggagctt
      241 ttgcctattt ttatccctgt caacaccgcg aattaaggtc ttttcaacct taacaaaact
      301 tggagacagg gctagcagga tgatttcact gttgtatccg gcgccaaaat catccagggc
      361 cagatgaata ttccagcgct tggctattga ctgttttttc cttatggtct cctcgttagc
      421 ctgttcattt tcggtgactt caattacaat gcgatgcaag tctcttttat attctcgttc
      481 aaaacgcctt aaatccttgt ccgttaacac ctgatttgca acggaattaa taaataatct
      541 tgcatcgtcg aaagctcccc tatgccgctg aaaggcttcc atagccttaa accacgtaat
      601 ccgttcaata tggtacagtt ttgattgggc agcagccagt ttgataatat cctcgggaga
      661 agtcaatgtc ggcactttgg gccgcatcaa cgcttcatat gcaaatactt ttccttcccg
      721 cgcatccaca ataggttcaa acacaaaatc caccagttca ttttcaataa aattgtccag
      781 ctcttcgttg ccatgtagga gaaaatactt gctttcatag ctttttctgt cgaactcgcc
      841 gatggttccc ttatcttcac tttttacctc gtacatggca aaatcggaat acttcatgag
      901 ctcatagtag tttttcgagt cttcagggta ccaggctatg cctaccgatg ccttgatgcg
      961 caggtcgccg ccattgggca tcctgatcgt cgtgttgtag agctttttct gtatcatttc
     1021 aaccaagtcg tttatttgtc gcttatcatc atagccgtat ataaatgcat aaaactcgtc
     1081 ccctgacatt ctcgccacaa caccgttgta gattgtcagt tcacttaaga cttttgctgc
     1141 atccttgata tactggtcgc caaaatcatg gccgtacgta tcgttgatgt actttaaatt
     1201 gtctaaatcc cacataacaa aagcagctat ttttacattt tccttcctca gtatcctggt
     1261 cacttttgct tcaaaagccc ggcgattcaa cagatgtgtc agagagtcgt ggtcacggtc
     1321 atactcaatt ctgcgcttct cgagcacttc cctgctgaca tcctctataa ctcccaggac
     1381 tttgccacca tgctcttgga tgttaatgcg cagccacttg atgactcctt ttcctctttc
     1441 atgccgatat acgtcttcca tgtcaggttc gggacgcttt ttcaaaccct ccagcacttc
     1501 ataaaaatgt gttgaactga tatatcttgc ttctttattc ttgttctcta ttcccaccag
     1561 gtcaaaaaat gcagaggtgc aaaaaaccat gtcttccttt gagtcgtgtt caaaggcacc
     1621 tatggggatc ttcatcatgc cgataattct tgaaagctta tcccccgact cagcaacttt
     1681 actgctgagg ctctcaattg acgatgccaa atcatctatt tcggcaatgt ttgtcttggg
     1741 gaggacaacg gatttttccg gattgctgct cctcagatca tccaccaatt tgattatggg
     1801 ctttacaaac cacatgccgg ccaataccgc tcccagcata ccggcaatga atgaaaagac
     1861 aacagatatt gtaacgtaaa gccttatttg ccttgcaggt ttaaacaggg tttgctcctc
     1921 tacaattcct acaagggccc atctgtcccc ttcaaatggg gtgttgctat cgtatatgtc
     1981 cagatagtgg atgcagccgt acacggcatt cttgctagct tcagtattgc ttatggtata
     2041 aatgtcttcg tactgtgtgc tcccgtctat tgctacctcg gtgtcatctc caaaaatttt
     2101 tttgaaaatc ggtccgctgg agactatgtt ttcaaaggcc atgtcttctc cagtattttt
     2161 ggctaaaagg tacgccccct gcttgttgtc agcaatttca tcataattta gcaacttgcg
     2221 caagaaatct tgggtcaggt caatcccgat gacaccgtaa ggatttccat cagagtccac
     2281 aaggggtatg gaatacgtta ttgcctccgc actgtatatg ttgggtttga agggtctgcc
     2341 ccaatatcct aaatcggata ttcggctgct cctgttgtta gctgctgcat tcaacggttt
     2401 aaaataaaaa tcagcggaag catcatcttc taacaatgga aaactcggca tccagcccat
     2461 acctagggca atatcagcct ttcttgcaac cgccgaaggg ccaacttcca tgctcagatc
     2521 cgaattgtct gcaggattaa acaaggggtc cgaatccctg aggtaaactc cgggatattc
     2581 atttccactt tctgttgcaa gtacaataaa agcaccagtg accattcgct gcctcagcgt
     2641 aaaaatcgta tcaccgatag tcctctccaa gaattcaaag gctaaagcgg gttccttttt
     2701 cagggttgaa agttctatat ctttttcctc cagataagaa ttcatggcgg attggatatc
     2761 ctgttcaaaa tttgacaggt ttgaccacct ttgaatcatt tcattttcaa ggtaatcctt
     2821 cctgctaagc accgtttgtc tcagcaggtc taccgagttg ttgtttagct ctctaattgt
     2881 ccctccgtgc caaatattgc cggcaaacaa tgaagtttgg actaatatca ccagcaacat
     2941 ggatagcagc aatcgaacaa ttatggaatt gccctttatc tttctcatat tcatatactg
     3001 tagccccacc ttttaactgc cgccatttat tgcggccgct aagctctctc ttaaacccct
     3061 tagccactcc tggaagtttt cctccgtagc tacctctttc accgcttcct ccctggtatt
     3121 tcctttttca agcagctcaa tgaccttctc cctgtctgcc tttgatttat ctataagcgc
     3181 ctttgacaaa acctctctgg cattcgtacc gttttcaaag gggtcgttag tgtaaagctc
     3241 gtagctctca agctgctcca tcagcaccgg cagtgaggat ttcatctttt ctgtcaccct
     3301 gttgtctttt gcatcggcca aaaatttttg gataactctt atgtcattgg cttctttttt
     3361 caccggcaga taaccggatt ccagggagaa tctgatgttc ctgtctatgt cggtgaacca
     3421 tttcagaaac tccaccgcag cacgctctct aagcttatca gacttgacca caaccatgcc
     3481 ggctccctgc tgaaccgcaa agggagctgt gttttcaaag ttgggaaggg gaagcaccaa
     3541 tgattcaatg ggatattctt ccctgtcatc aattattacg gtgtcaggaa agtagggaat
     3601 tgctgaagtg gagcatacta gggctattat ctctccggtc ttggcatcat cggagcggaa
     3661 tctgccgacc gccgtatagt atccgcttat ataaggtatg tagaaattgt cccagagccg
     3721 cctcataatt ccttcatcaa catgaataga tacctgtcca ctttccacgc tgaaaatttc
     3781 ttgccctaac tgttttgaac ctacgatgat ataatttgcc atggcatccc ggccgaaaaa
     3841 ggcctttccg tcattgggca cagctgtcaa actgtccgtc cactcgtagt atttttcagc
     3901 ggctttagcc aagccttccc aggtcctaag ctcttccaag cttgctcccg tggcatttgc
     3961 gaatttgtcc caatcggttt tgtttagcat caggacttca gtggccttgg cagtgggaaa
     4021 tatcttgatt ccgttgccat cgccgaagcg cccctcccgg atgtacgagt ccacgtactc
     4081 atcgatttct tcggggctaa ggtactcgct tatatcagcc acatggccca attggtcaat
     4141 ttgatatgca ttgtcagcat atgcagcaaa tatgtccggc atgggctccg ccccaatttt
     4201 tttggtaaca gactccatta cctttctctg caaggcatct acattgccct ggctaaaagc
     4261 ctccactatt atgcccttct ccgcacccaa ggtctcgtta aattccataa ccagtgcatc
     4321 aaaagccgtc ttctgaagcc cgttatagtg gtgccatatc tcgattgaaa tggggttttc
     4381 agggtccaag ccatctttgt ctgcttttga gcaagctgtc aaagaaaagc ttatgcacat
     4441 gcacaaaatc aatagactga taagccgtcg acactttttt acacctttca tgtaattacc
     4501 tcatattcca acaatttttt taattaaaat agtttacatt aataatatca ttattggcca
     4561 agtctggcaa tctgaatcgt tcggaataat tccctcgcag gggtaaaact cctttggaat
     4621 ttgcttgtac aagaaaaatg tcgcaaaact actattaagc agtcttgcga cactcggctt
     4681 tatatcaagc gatgcagcaa ttgcaccctg tctccatgta aaaagtctat gataggtatt
     4741 tcaagcaggg tatagcagcc gggtctttgc cttaagctgg ccctagcggc agccaccatg
     4801 atttggccgg ctgctgcggg attgcaaatt gacatgatga actcaattcg ctggttatgg
     4861 gtggtgccgg atacaccctt cctttgaatg tgcactccgt gtcctttgtc aattaggctt
     4921 ttgacatcat ctactttgta cacatgggtt tcatcgtgga tgaaataggg gtctgttttt
     4981 acagcttccg cgattttatc aaaatcatat ccttctttta cctgcacata taccaggcgc
     5041 ttgtgtaagc cgtggccctg gggaatggtc atggaaaggg catcctctac tccttcaatc
     5101 gccttgacca ctacggtatg ccctaagctc atgcccggcc cgaaattggt ataagtgagg
     5161 cccctagggg caactacctc taccaaagcc cttacaatgg aatccgaccc cggatcccag
     5221 cccgcggaga taactgctac ggaatcgtga gctttggctg cctcgtcgag tttttccctc
     5281 aagctgatga cgtcatcgcc gtgaatatca aagctgtcaa cggtgttaat gcccatttcc
     5341 agaaagcggg gtgcgacttc agcaatggag cgactgggta tgctgagcac tgccacatca
     5401 acctttccca gttccctaac atcggtggtt accggaacat cccccacaat cttttttgtg
     5461 gcctggattt tcttagggtt cctaatgact attcccgccg tttccatatc cgggctctcc
     5521 tcaattgcat gaaaaacttc tctgcccaca ttaccaaaac ccactactgc aatcctgtat
     5581 tttgacatgt ttaacctcct cctgctattt acaataaaaa aacaatcggg ctatttgccg
     5641 attgtcgtta aagccattgg caaatacctc tctcctgaag tagctctcca ccggattgtc
     5701 cggtgacagt cctataccta ttcgatatag gcccagcatg atctttggga gaaaccatgc
     5761 ttcggcggtg acccctttta gcgtgcttaa tcggctcccc
//
LOCUS       DAONUO010000108         5768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10310_length_5768_cov_3.718187, whole genome shotgun sequence.
ACCESSION   DAONUO010000108 DAONUO010000000
VERSION     DAONUO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10310_length_5768_cov_3.718187"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..878
                     /locus_tag="PK768_05135"
     CDS             183..878
                     /locus_tag="PK768_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQA60387.1"
                     /translation="MVSLEDKLIHFVNIEAEEDALSIYRRIVNVIISKELEPEARLRY
                     MKNYLVSLNSIMYVNCRKGLVCIRKLINMRDSIMNEIEARQTLEDVILVGEEMVKRYL
                     NFINDQLCRINNPIINDALAYIKNNLDKELSLDEVSSAIHVSKSYLSNLFSKCIGNSF
                     SHHVNKMKIEKAKELLAKTKLSIMDITLECGFNSQSYFCRVFNGFEGMTPNQYRKLYS
                     EHRHQDGKELNAL"
     gene            complement(873..1442)
                     /locus_tag="PK768_05140"
     CDS             complement(873..1442)
                     /locus_tag="PK768_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQA60388.1"
                     /translation="MLKFKFKERNLSHIVPNAITFANMALGVIAIYLSMGATVDKIKA
                     AAAIILIAGAADKLDGFAAKKLGSVSEFGKELDSLCDLVSFGVAPILVWWNMGMDSLN
                     FAVCTVSLLYIGAGAYRLARFNLGKDKGYIVGLPITIAGMAMAGKVLIDISFRLEYIT
                     KTILMLENLGLTLLLSLLMASSFKIKRPL"
     gene            complement(1423..2430)
                     /locus_tag="PK768_05145"
     CDS             complement(1423..2430)
                     /locus_tag="PK768_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQA60389.1"
                     /translation="MKREYKLVLYALGAFMLGYIVLSADWIEFYTYLQVTPVKVIVLL
                     LFYQCITKLLINIQWKSLASEVCGEVSFLDVVLANATGDIMDGLTPGVKMGGELGRIF
                     ALKSRADIDIADASIIVGLQKTFSLFSFLLLTLGSVTWFSLSFGGSDKYYLYIFTAAI
                     AAFIVIFAALILFVLKPETIMGRLGRISRGSKTIIKIEESLKKYSQILRRLFSNKRLF
                     CSQMLLGIFIWSFYAFKLHVLVKSYHIQMGFFSLAAITFLSYAMGMIPLLPGGMGSFE
                     SCMILLLKLSGISVEMGAAIAIVFRLVTFWFEFILSGLILILASVPAICRGDNDVKIQ
                     V"
     gene            complement(2440..3006)
                     /locus_tag="PK768_05150"
     CDS             complement(2440..3006)
                     /locus_tag="PK768_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030410 - nicotianamine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0030418 - nicotianamine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotianamine synthase family protein"
                     /protein_id="HQA60390.1"
                     /translation="MIAVCTECIERNFSKSNFLIKLYGLYYRNIVKNEVKLAGIRSCD
                     RILCIGGGPIPSTAIEMARQTDACVHVIDMDGTAVVCARDVIKKLGLAHKITVEQARG
                     EEVDISPYNVIHVALQVRPKEAVLRHIWNNCKLGARIIVRMPKKGLRSFYSNVTDAFI
                     ERNSDYVSFDSSGLCANTMDKTLLMVKA"
     gene            complement(3022..4779)
                     /locus_tag="PK768_05155"
     CDS             complement(3022..4779)
                     /locus_tag="PK768_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HQA60391.1"
                     /translation="MRCHSSRPHEKNIENEKIRILLMGNPNVGKSVIFSKLTGVNVVS
                     SNYVGTTVDYTVGDVSFGRQRGVMIDVPGVYSLEATSEAEKVAVSLLEEGADVIVCVL
                     DATHLERNLLLANQLREYQIPIVYSLNLLDVAERQGIKIDAAKLSEELGAPVIPTVAI
                     KNAGLMDLLRKAVSFAKEKAKPEPKLSDEERWLKIKDIVAKVQTEVEKKTTFMDLLED
                     KTIQPWPGIPIALLVLILSLGVVIGGGKGLRALVLLPLVRDVIQPFLTNIVSLLVPPG
                     IFRNLLVGEFGIFIIGIEWPFALILPYVFLFYIVFSFLEDSGFMPRIAVLADGVFRRI
                     GIQGGNIIPIMLGYGCAVPAILGSRTANTYKERLIVAALVSFAVPCASQSAAFFALLG
                     DRSVAALAFVYLLSILMGLFVGYVMNRMVPGKSQPMLLEVPNLLWPDKEAFKKKLKIR
                     MKGFLVDAEGPMLMGIIIASLIAETGVLNEFSKLIGPLVEGWLGLPKEASLSLLLGII
                     RRELAVLPLLELNLSTLQLVVGSVIALFYLPCLSVFAVLAKEFSLKSAVFIGVLTTTF
                     AFIFGGIINKLGGLILGVL"
     gene            complement(4781..4996)
                     /locus_tag="PK768_05160"
     CDS             complement(4781..4996)
                     /locus_tag="PK768_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQA60392.1"
                     /translation="MSLTFGSKRNKYRVVELPDSHLLKVLGLRKGTEFTLQTKHPFKG
                     PVVVKVGCRCIAIDHDIARDIIVEEVS"
     misc_binding    5225..5485
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1686 a         1390 c         1197 g         1495 t
ORIGIN      
        1 taaaaaatgc caacaacact tttttgaata aattgggaaa ggtacaaaaa attcatcggg
       61 atgaattttt tgtacctgtc cccaattatt cacaaaagtg ttgctggcat cttttaaata
      121 taactggtat aatataaatg aaaaccattt tcatttgaag ccgcataaac gggaggtaaa
      181 taatggtttc tttagaagat aaattgatac actttgtcaa catcgaagct gaagaggacg
      241 ctttgagcat ctacaggcga attgtaaatg ttatcataag caaagaactt gagcccgagg
      301 cgagattgag gtacatgaaa aactaccttg tttccttaaa cagtataatg tatgtcaatt
      361 gccgcaaagg gctagtatgt ataagaaagc ttattaacat gagagatagt atcatgaacg
      421 aaatagaagc acggcaaact ctagaggacg tgattcttgt aggggaagag atggtcaagc
      481 ggtaccttaa ctttataaat gaccagcttt gcaggataaa caatcccata atcaacgatg
      541 ccctcgcata tataaagaac aaccttgaca aggagctcag cttggacgag gtatccagtg
      601 ctatccacgt cagcaagagc tatctctcca acctgttttc aaaatgcata ggcaatagtt
      661 tttcccatca tgtcaacaag atgaaaattg aaaaagccaa ggaactcctg gcaaaaacaa
      721 agctttcgat aatggatata acactagagt gcggtttcaa cagccaaagc tatttttgcc
      781 gagtattcaa tggctttgaa ggcatgactc caaaccaata tagaaaactg tacagcgaac
      841 acaggcatca ggatggaaaa gagctcaatg ccctataggg gcctttttat tttaaaactg
      901 ctggccatca ggagggacag caaaagggta aggccaagat tttcaagcat taagatagtt
      961 ttagtaatat attccaatct gaaactgata tctatgagga ccttccccgc catggccatg
     1021 cccgctatgg ttatgggcag tcctacaatg tagcccttgt ctttgccaag gttaaaccta
     1081 gccaacctgt atgcaccggc accgatgtaa agcagggaca ccgtgcacac ggcaaagttc
     1141 agtgaatcca tgcccatatt ccaccaaaca agtatcggag caacaccgaa ggaaaccagg
     1201 tcgcaaaggg agtccaattc cttgccgaat tcgctaacgg agcccagttt tttggcagca
     1261 aaaccatcca gtttatctgc agccccggct atcaggataa ttgccgcggc tgcttttatt
     1321 ttgtctaccg tagcacccat gcttaaatat atggctatga cacccaaggc catattggca
     1381 aaggttatgg cattaggcac tatgtgtgac agattccgct ccttaaactt gaattttaac
     1441 atcattatca cctctgcaaa ttgcagggac actggccaag attaagataa ggccgcttaa
     1501 gataaactca aaccagaaag tcacaagcct aaacactata gcaatggctg ctcccatttc
     1561 cacggagatg cccgagagct tcaggagcag tatcatgcag ctttcaaaac tgcccatgcc
     1621 gccgggcagc aggggaatca tgcccattgc ataagataaa aaggtgatgg ctgccaggga
     1681 aaagaaaccc atctggatat ggtaactttt caccagcaca tgcagcttaa aggcataaaa
     1741 actccaaatg aagattccca aaagcatctg cgagcaaaat agccttttgt tgctaaaaag
     1801 ccttcttagt atctggctgt acttctttaa gctttcctca atcttgatta ttgttttaga
     1861 acccctagat attctgccca gccgccccat gatagtttca ggttttagga caaataatat
     1921 tagagcggca aatatcacta taaatgccgc aattgccgca gtgaatatat ataaatagta
     1981 cttatcgctt ccgccaaagc tgagactaaa ccaggttaca ctccccaacg tcaagaggag
     2041 gaaactaaac aggctaaagg ttttttgcag gcccacgatt atgctggcat cggcaatatc
     2101 gatatctgcc cggctcttca gcgcaaaaat ccttccaagc tctcctccca tcttaactcc
     2161 gggcgtgaga ccgtccatta tatctcccgt agcattagcc aggactacat ccagaaaaga
     2221 gacttcgcca cagacctcac tggccaggga tttccactga atgttgatca ggagcttagt
     2281 gatgcactgg taaaacaaca gcagcactat aacctttacc ggtgttacct gtagataggt
     2341 gtagaactct atccaatcgg cacttagcac tatatagccc agcatgaaag ctcccagggc
     2401 atacaggaca agtttatatt ctctcttcat agtcatcctc taagctttca ccatcagcaa
     2461 agttttatcc atggtattgg cgcacaagcc ggaagagtca aagctgacgt agtcggaatt
     2521 tctctctata aaggcatccg ttacatttga ataaaaggac ctgaggccct tcttcggcat
     2581 gcgcacgata atccttgctc ccagcttgca gttattccag atatgcctta aaacggcctc
     2641 ttttggccta acctgcaagg caacgtggat tacattgtac gggctgatat ccacttcttc
     2701 tcccctagcc tgctctaccg taatcttatg cgcgagaccc agcttcttta ttacatccct
     2761 ggcacatacc acagcagtgc cgtccatatc aatgacatga acacaggcgt cggtctgcct
     2821 tgccatttct atggctgtag agggaatggg cccgccgccg atgcaaagta tcctgtcaca
     2881 gcttcttatt ccggccaatt ttacctcgtt ttttacaata tttctgtaat acagcccata
     2941 cagctttatt agaaaattgc tcttggaaaa gtttctctct atacattcag tgcaaactgc
     3001 tatcatacta agcctctccc atcacaatac tcccagtatc aggccgccca gtttattgat
     3061 tatcccgccg aagataaagg cgaaagttgt ggtcaataca cctataaaga ctgcagattt
     3121 cagcgagaac tcctttgcca gcaccgcaaa taccgacagg cagggcaggt agaatagagc
     3181 tattaccgaa cctactacca gttgcaaagt gcttagattt agctcaagca aaggcagtac
     3241 cgccaattcc cttcttatta tcccaaggag cagagataag ctggcttctt tcggcaagcc
     3301 tagccagcct tctaccaggg ggccaatcag cttgctaaat tcattcagca cacccgtttc
     3361 cgctatcagg gatgctatta ttatgcccat cagcatgggc ccttctgcgt ccaccaggaa
     3421 acctttcatc ctgatcttta gctttttctt aaaggcttcc ttgtcaggcc acaagagatt
     3481 tggcacttca aggagcatcg gctggctctt gcccggaacc atccggttca tgacatagcc
     3541 tacgaacagg cccatgagta tggacaggag atatacaaac gccagggccg ctaccgacct
     3601 gtcgcccaat agggcgaaga aggccgcact ttgagacgca catggaacgg caaaggaaac
     3661 caacgccgcg actataagcc tctccttgta agtgttggca gtcctgctgc ccaaaattgc
     3721 aggaacggca cagccgtaac ccagcattat gggaatgata tttccccctt gaatgcctat
     3781 gcgcctgaaa accccatcag ccaaaaccgc aatccggggc ataaacccgc tgtcttccag
     3841 gaaggaaaag acgatgtaaa ataaaaaaac gtagggcagt ataagggcaa agggccactc
     3901 gatgccgatt atgaaaatcc cgaactctcc caccagcagg tttctaaaaa tgccgggggg
     3961 cacaagcagt gacactatat tagtgaggaa aggctgaatc acatccctca ccaaaggcaa
     4021 aaggaccagg gccctcaatc cctttcctcc gccaattacg acacctaaag acaaaataag
     4081 gaccaatagt gcgataggta tgcccggcca gggctgaatc gtcttgtctt ccaacagatc
     4141 cataaaagtg gtcttctttt ccacctcggt ctggaccttg gccacgatat cctttatttt
     4201 aagccatctt tcctcatcgg aaagctttgg ctcgggcttt gctttttcct tggcaaagct
     4261 tacggccttc ctcaaaagat ccattaagcc cgcgttctta atggcaactg taggtattac
     4321 gggagcaccc agttcttccg acagctttgc cgcatcaatc ttgatgccct gcctttcagc
     4381 cacgtccagc agatttaaac tgtagactat gggaatctgg tactctctta actggttggc
     4441 caaaagcagg tttcgctcca gatgggtagc atctagtacg cagacgatga catctgcccc
     4501 ttcttctagt aaactcaccg ccaccttttc agcttcggaa gttgcctcta aggaatatac
     4561 accgggcacg tctatcatga ctcctcgctg ccttccaaag cttacatctc ccacggtgta
     4621 atctacagta gtgccgacat agttggaact tactacgttg actcctgtca gctttgagaa
     4681 gataacgctc ttgccgacat tggggtttcc catgagcagt atccttatct tctcgttctc
     4741 tatgtttttt tcatggggcc ttgatgaatg gcacctcaaa ctacgaaacc tcctccacga
     4801 tgatatccct tgctatgtcg tgatctattg ctatacatct gcatccaact ttaacaacaa
     4861 ccgggccttt aaagggatgc ttggtctgaa gggtaaactc cgttcccttt ctaagtccca
     4921 gtacttttaa caagtgagaa tcgggcagtt caactaccct gtacttattg cgtttgctcc
     4981 caaaggttaa agacatcata tcttcctccc tttattaagg cattgagaat gtttatcttt
     5041 ctcaattgta attatagtat caaccaaaag gcattttttt aaatttcttt tacttatttt
     5101 taaaaaataa tcactttttg tcccacgcaa catcaagaat tgcacattct ggcttttatt
     5161 gcaaaaaaac ttgatttttg ttcttgacag tcgtctcctt tctcggtata atgtcttcaa
     5221 tattttaaaa actatgaccg agaagaagta ggaacgcgcg agccttaaag agagttgccg
     5281 gctggtgaga ggcgcaaggg gaggcgtatc cgaatacatc tcaggagttg ctcaccgaaa
     5341 ggaaagaccc gagtagactg ggccgggggc ctgcacccgt tacagtgcta gagtatattg
     5401 ccattgcaat gtacttgata aggttggtat cgcgagatat caatgaatta aggtggtacc
     5461 acgggaacac taacccgtcc tttacatatg aggacgggat tttttaattt gtatgaaagg
     5521 agagggttgc tattagggtt tgtaaaacgg taggatggta aactgtagga tggtagcatg
     5581 gcttttatag tataaaaaaa tggaggatgg taatatggca aacatcaaat tttttaacaa
     5641 tgaaaaagta cccttggaaa tgcacaaggt aaggattgtg caaaaactgg agctaaggcc
     5701 catcgagcag cgcctcgagg caataaatga ggcaggaaac aacactttcc ttctcaaaaa
     5761 ccgggacg
//
LOCUS       DAONUO010000109         5766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10313_length_5766_cov_4.013833, whole genome shotgun sequence.
ACCESSION   DAONUO010000109 DAONUO010000000
VERSION     DAONUO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10313_length_5766_cov_4.013833"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PK768_05165"
     CDS             <1..641
                     /locus_tag="PK768_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HQA60393.1"
                     /translation="KAEIIDSNPTELGGFKEVVFEINGNGAYSRLKYESGVHRVQRVP
                     TTEAGGRIHTSAATVAVLPEAQEIDIDINPNDLRIDIFRASGHGGQGVNTTDSAVRIT
                     HIPTGMVVTCQDERSQLKNKEKAMKVLRARLMEKAQMEQNQKIAQDRRSQVGSGDRSE
                     RIRTYNFPQGRITDHRIGLTIHQLTSVLEGELDEIIDALIAEDQAKKLAQLE"
     gene            656..1510
                     /gene="prmC"
                     /locus_tag="PK768_05170"
     CDS             656..1510
                     /gene="prmC"
                     /locus_tag="PK768_05170"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQA60394.1"
                     /translation="MIVREALNIGKKRLHEAGVANPDLDSQLLLSHVLRTDRLKLFVY
                     DKTELTKEQLESYDKLLNERCRFVPVAYIIGRKEFYGLNFYVKEGVLIPRPETEFVVE
                     EALKAAGQISNPVIADLCCGSGAISVAIAVNNKQAKVYAVEISEVACEVARQNINAHG
                     VQDRVLLLKADLWTALEERKLTGLDIIVSNPPYIPSQELNSLPRDVKNEPQLALDGGP
                     DGLDFYRRIISRAPEFLKAGGRIILEIGWNQAPDVKKLLEKIGFIQVKIRKDYAGFDR
                     VVTGILGL"
     gene            2113..>2481
                     /locus_tag="PK768_05175"
     CDS             2113..>2481
                     /locus_tag="PK768_05175"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQA60395.1"
                     /translation="MPKTQIITVDKNQPERDKIKRAAEIIRNGGLVAFPTETVYGLGG
                     NALDEDAARKIYAAKGRPQDNPLIVHIADISQLDELVKEIPERAKLLMDKFWPGPLTI
                     IFNKSGLVPFGTTGGLDTVAI"
     assembly_gap    2482..2491
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2492..3145
                     /locus_tag="PK768_05180"
     CDS             <2492..3145
                     /locus_tag="PK768_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HQA60396.1"
                     /translation="ALELIKEAAVPIAAPSANISGRPSPVTAQDVAEDLVGRIEMILD
                     GGKASVGVESTVLDLSEELPIILRPGGVTREELEEVLGRVDLDPGLAPGEKPRSPGQK
                     YRHYAPKARMTLFEGPLDNQVRAISEMAEKLEVEGLKVGIMATAQTRHLYTKGQVISA
                     GDRSAPLTISSNIFGVLRKFDRMGVDRILAESISQDGIGLAVMNRLYKAAGFNVLKV"
     gene            3161..3610
                     /locus_tag="PK768_05185"
     CDS             3161..3610
                     /locus_tag="PK768_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HQA60397.1"
                     /translation="MKVVFVCTGNTCRSSMAEALFRKMLYDLGKHEQIEVESYGIAAF
                     EGQPASENAIKVMEMEGIDLKGHRARQFRQEALDADLILTMTQSHKDYILDRFPRAKG
                     KVFLLGEFVGDYAEISDPFGQGTDVYKQVAQELKEKLSKIIERLEKN"
     gene            3669..4112
                     /gene="rpiB"
                     /locus_tag="PK768_05190"
     CDS             3669..4112
                     /gene="rpiB"
                     /locus_tag="PK768_05190"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQA60398.1"
                     /translation="MSIAIASDHGGFELKQAIIKYFDKNEISYTDLGTYSTESVDYPD
                     IAISCAEAVARGEYEKGIIICGTGIGVCIAANKVKGIRAAQCHDTFSARMSRLHNDAN
                     ILTMGGRVIGSGLAIEIVKAWLASDFEGERHQRRVNKISQYESNR"
     gene            4183..5421
                     /gene="glyA"
                     /locus_tag="PK768_05195"
     CDS             4183..5421
                     /gene="glyA"
                     /locus_tag="PK768_05195"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQA60399.1"
                     /translation="MDILKLVDPEIADAIEKETHRQQYKLEMIASENFTSKAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDIVENLARERAKKLFSAEYANVQPHSGSQANMGVY
                     FAYLNYGDKVLGMNLAHGGHLTHGSPVNISGKYFEFIPYGVSKETGYIDYDELEALAK
                     EHKPKMIVAGASAYPRIIDFQKIADIAKQVGAYVMVDMAHIAGLVAAGLHPNPVPVCD
                     FVTTTTHKTLRGPRGGIIFCKEEYGRAIDKAIFPGIQGGPLMHVIAAKAVCLKEASTE
                     EFVEYQNQVVKNAKALAKALLDKGYNLVSGGTDNHLILVDVRNKKLTGKEAEHLLDEV
                     GITVNKNAIPYDPESPNVTSGIRIGTPALTSRGMKEEEMERIAELMDLALSHREDERV
                     KAKVSKEVKALCEEFPLYSK"
     gene            5438..>5766
                     /gene="upp"
                     /locus_tag="PK768_05200"
     CDS             5438..>5766
                     /gene="upp"
                     /locus_tag="PK768_05200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQA60400.1"
                     /translation="MAENVFVFDHPLIQHKLALIRDENTGSKEFRELVEEVSLLMAYE
                     VTRHMPLEETQVNTPVGPAKVKMLSGKKIGIIPILRAGLGMVNGMLKLIPAARVGHIG
                     LYRDPET"
BASE COUNT         1740 a         1254 c         1440 g         1322 t
ORIGIN      
        1 ggaaagcgga gatcatcgat tccaacccga cggaactcgg cggctttaaa gaagtcgtat
       61 ttgaaatcaa cggcaacggc gcttacagcc gtttaaaata cgagtcaggg gtccatcgcg
      121 tgcagcgtgt cccaaccacc gaagccggtg gcaggatcca tacatctgcg gcaacggtgg
      181 cggtattgcc cgaagcccag gaaattgata ttgacattaa tcctaacgac cttagaatag
      241 acatttttag agcctcgggc catggtggcc agggggtcaa caccacagat tcagcggtga
      301 gaataaccca cattccaaca ggcatggttg tcacctgcca ggatgaacgt tcacagctga
      361 aaaacaaaga aaaagccatg aaggttttgc gggcccgcct tatggaaaag gctcaaatgg
      421 agcaaaatca aaagattgcc caggaccgcc gcagccaggt aggttccggt gaccgcagtg
      481 agcgaatccg gacttacaac ttcccccagg gaaggattac cgaccacaga attggtctta
      541 ccatccatca gcttacaagt gttctcgaag gagagctcga tgagataatt gatgccctta
      601 ttgcagagga ccaggccaaa aagctggccc agcttgaata gaaaaggtgt tggaagtgat
      661 cgtaagggaa gctttgaaca tcggcaaaaa aaggcttcac gaggcaggtg tggcaaatcc
      721 cgatttggat tcccagctcc ttttaagcca tgtactgaga acggaccgac tcaaactttt
      781 tgtatatgat aaaacggagc tcaccaaaga acagctcgaa tcctatgaca aattattaaa
      841 tgagcggtgt cgttttgtac ccgtagcgta tataataggc cggaaggagt tttacggcct
      901 taatttttat gtaaaagaag gcgtgttgat cccaaggccc gaaaccgagt ttgtagtgga
      961 agaagcttta aaggctgctg gccagatttc aaatccggtg attgccgatt tgtgctgcgg
     1021 cagcggggca atttccgtgg ccattgccgt gaacaacaaa caagccaagg tttatgccgt
     1081 tgaaatctcg gaagtagctt gtgaagtagc aagacagaat atcaatgccc atggtgtgca
     1141 agacagggtc ttactgctca aagctgacct gtggacagcc cttgaggaaa gaaagcttac
     1201 agggctggat ataatagttt caaatcctcc ttacattcct tcccaagagc ttaacagtct
     1261 ccccagagac gtgaaaaatg aaccgcagct ggccctagac ggtggccctg acggcctgga
     1321 cttttaccgc aggataatat caagggcccc ggagttttta aaagcaggcg gcaggataat
     1381 cctagaaatt ggctggaatc aagctcctga tgtaaaaaag cttttagaaa aaatcggctt
     1441 tatacaagta aagattcgaa aagactacgc cggctttgac cgagtagtaa cgggaatatt
     1501 aggtttgtaa tgtttaactt tttgatagaa tggaagcaaa aaccaaaacc atgcaaataa
     1561 gggtttctca ttaaacatga taaatctaaa tctgcaaaga actcacccta taaccatggg
     1621 ttttaccaac atacttctta aactaaaaat actgaaacgt gattagatat catcatgttt
     1681 ttcaaatatt gtttttcgtt aacatatcca tttcatatat tatagaatcg atcaagaatt
     1741 ctacatgata ttagtccttg gtgaagaaaa cggcacctct tccagtagtt agacctgtca
     1801 ttcaaaaatt tggtcctgaa ctccgcaggg tttcacgaag ctatgcggca tcctgccgag
     1861 agcccgtcag cgtgcgtcct gcacgctgcc cctgctccgc ttggcccaaa tttaagaatg
     1921 acaacggtct aactaatggt agcgtttgcc gtcttcttca ccaagcaaag aactgcagtg
     1981 aaaaggatgt atagtaataa taggagtaat aataaaaaat ctagcaaata agaaaccttg
     2041 taataacgag atatgtggca aaaattatat caaaataatt ccagcaaaat caatgaaaaa
     2101 gaaggtaatt ctatgccgaa aacccagatt atcaccgttg ataaaaacca acccgaaagg
     2161 gacaagataa aaagagcggc tgaaatcata agaaatggcg gcctggtggc ctttcccacc
     2221 gagaccgttt acggcttggg aggcaacgcc cttgacgaag atgctgccag aaagatttat
     2281 gccgcaaagg gcaggccaca ggacaatccc ctgatcgtcc acatagctga catctcgcag
     2341 ctggatgaac tggtgaaaga aataccggaa agggcaaaac ttttgatgga caagttctgg
     2401 ccgggccccc ttaccattat atttaacaaa agcggtctgg tcccctttgg cactacagga
     2461 ggccttgata cggttgcaat tnnnnnnnnn ngccctcgaa cttatcaagg aggcagctgt
     2521 tcccatcgca gctcccagtg ccaatatatc cggcaggcca agtcccgtaa cagcccaaga
     2581 cgtcgccgaa gatctagtcg gaaggataga aatgatactg gacggaggga aggcttcggt
     2641 gggggtggaa tctaccgtgc tggacctttc ggaagaactg ccgataatat tgcgtcccgg
     2701 tggagtcacc cgggaggagc tggaagaagt cttgggcagg gtggacctgg accccggcct
     2761 tgcacccggc gaaaagcccc gctctcctgg gcaaaagtac cgccattatg cacctaaagc
     2821 tcgcatgacc ctctttgagg gtcctcttga caatcaggta agagctatca gcgaaatggc
     2881 ggaaaagtta gaagtggagg gacttaaagt tggcataatg gccactgccc agacccgcca
     2941 tctttacaca aaaggccagg ttatttctgc aggtgacaga agcgcgccgc tcacgatttc
     3001 atccaatatt ttcggcgtgc ttaggaaatt cgaccgcatg ggagtcgacc ggattctggc
     3061 ggaaagcata tcacaagacg gcattggcct tgcggttatg aacagacttt ataaggctgc
     3121 cggcttcaat gttttaaaag tataggaggt gtgtcccccc gtgaaagtgg tatttgtgtg
     3181 taccggcaat acctgccgca gtagtatggc ggaagcccta ttcagaaaga tgctttacga
     3241 tttgggaaag cacgagcaaa ttgaagttga atcttacggc attgctgctt ttgaaggtca
     3301 accggcttct gaaaatgcga taaaagttat ggagatggaa ggcatagact taaaagggca
     3361 tagggctagg cagtttaggc aggaagccct ggatgcggat ttaatcctta ccatgaccca
     3421 aagccacaag gactatatac tggatagatt tccccgggcc aaggggaagg tgtttttatt
     3481 gggagaattt gtaggtgact atgccgaaat cagtgacccc ttcggccaag ggacagatgt
     3541 ctacaaacag gttgcccagg aacttaaaga aaagttgtca aaaatcatcg aacgcctgga
     3601 aaaaaactag acaatatcca ttatttaagt atataattat tagtatgaag ttttcaggag
     3661 ggaaagagtt gagtatagct atagctagcg accatggcgg ttttgagtta aaacaggcca
     3721 taataaagta tttcgacaaa aatgaaatca gttataccga tttgggaacg tactctaccg
     3781 aatccgtaga ctatcccgat atagcgattt cctgtgcaga agccgttgcc cgaggcgaat
     3841 acgaaaaggg cattattatt tgcggtactg gaataggcgt ctgcattgcg gccaacaagg
     3901 tcaagggcat tcgagcagcc cagtgccacg acacattctc tgcccgcatg tcccgcctgc
     3961 ataacgatgc caatatctta actatgggcg gtagagttat aggttcggga cttgccatag
     4021 aaatagtaaa agcatggctc gccagcgatt ttgaaggtga gcgccatcag cggagagtaa
     4081 acaaaatatc ccaatacgaa agtaacaggt aaaagtgaat ggtcagccta gtataggaac
     4141 ggcctgtgcc gtctaaacca tagaaaaaga ggtgctttgg agatggatat acttaaattg
     4201 gttgatccgg aaatagccga tgcaattgaa aaagaaactc acaggcagca gtacaaactg
     4261 gaaatgattg cttctgaaaa ttttaccagc aaagcagtaa tggaagccca gggttcggtg
     4321 ctcaccaata agtacgccga aggctatccg ggcaggcgtt actatggcgg ctgtgagttt
     4381 gtagatattg tggaaaatct tgcccgggag cgagcgaaaa agcttttttc cgccgaatat
     4441 gccaacgtcc aacctcactc cggttcccaa gccaatatgg gtgtctattt tgcttacctg
     4501 aattacggag ataaggtttt aggcatgaat ctcgcccatg gtggccacct gacccatgga
     4561 agccccgtaa acatttcagg aaagtacttt gaatttatac cctacggcgt ttccaaggaa
     4621 accgggtaca tcgactatga tgaattggaa gctttggcaa aagagcataa accaaagatg
     4681 attgtagctg gtgccagtgc atatcccaga atcattgatt ttcaaaagat agccgacata
     4741 gccaaacaag tgggcgcata cgtaatggtg gatatggcac acatagctgg ccttgttgcg
     4801 gcaggtctcc atccaaatcc ggtgcccgta tgcgattttg tcaccactac aacccacaag
     4861 accttaaggg gtccaagggg cggcattatc ttctgcaagg aagaatacgg gagagccatc
     4921 gacaaggcta ttttcccggg aatccagggc gggcctttga tgcacgtcat cgccgctaag
     4981 gcagtttgcc tgaaagaggc ctcaacagag gaatttgtgg aatatcaaaa tcaagtggtt
     5041 aaaaacgcca aggccttggc taaggcactt ttggacaagg gttataacct ggtttccgga
     5101 ggtaccgata accatctgat tttggtggat gtgagaaaca agaagctgac cggaaaagaa
     5161 gccgaacatt tattggatga agtggggatt acggtaaaca aaaatgccat cccttacgat
     5221 ccggaaagcc cgaatgttac gagcggcata cgtatcggaa cgccggccct tacatctcgg
     5281 ggaatgaaag aagaggagat ggaaaggatc gcagagctaa tggatctagc cctaagtcat
     5341 agagaagacg agcgcgtgaa ggcaaaggta tccaaggaag ttaaagcttt atgcgaagag
     5401 tttcccctct attccaagta aacaggaggt ctatttaatg gcagaaaatg tgttcgtatt
     5461 tgatcatccc ctaattcagc acaagttggc tttaataagg gatgagaata ccggttcaaa
     5521 ggaatttcgg gagcttgtgg aagaagtatc cctccttatg gcttacgaag ttaccaggca
     5581 catgccttta gaagaaaccc aggtgaacac tcccgtagga cctgcaaagg tgaagatgct
     5641 atccggcaaa aaaataggta taatcccaat acttcgggca gggctgggga tggtaaacgg
     5701 tatgttaaaa ctcataccgg cagcaagggt cggccatata ggcctgtacc gggatcccga
     5761 aacctt
//
LOCUS       DAONUO010000110         5755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10333_length_5755_cov_3.664211, whole genome shotgun sequence.
ACCESSION   DAONUO010000110 DAONUO010000000
VERSION     DAONUO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10333_length_5755_cov_3.664211"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PK768_05205"
     CDS             complement(<1..1225)
                     /locus_tag="PK768_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HQA60401.1"
                     /translation="MENDQAQIVDERCITCGTCLTICPQNAKTIKSDIQRVKELLKID
                     KDIAVSLAPSFAGVFHFNHYSQIVSAIKRLGFSNVYQTSVGARLIASDYLKHYNDRTK
                     PNLITTACPAINYLVQKYYPELVECLVPVVSPMEAHGRYLKKIKGHKEVAFIGPCLAK
                     KVEIHDNGNYGIDAALTFEELTAWWKEEGIDPILEPVNEQDNGFCDDANFYPIPGGSY
                     KTIKPLIEDPWREFIEVSGKENCMLMLDELRKGEFHRTWIEMNACEGGCINGPAIDII
                     DRGPFKRIKCVKIFARHNSPCKESISISGQGLSFDFEKKFEPTPIKTKKPSEQDITRI
                     LEATGKYRPEHELNCGACGYNSCREKAEAVYNGMAEIHMCLPYMRNRAESLSNLIIQS
                     TPNAIIAVDKDINVQV"
     gene            complement(1311..1553)
                     /locus_tag="PK768_05210"
     CDS             complement(1311..1553)
                     /locus_tag="PK768_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQA60402.1"
                     /translation="MRTISVCVGSSCHLKGSYDIIRIFKALIKEHGLEDKWELKAEFC
                     CNNCRNPVSVRVDDSEPYSVDLESAEAFFKEKILGA"
     gene            complement(1691..1936)
                     /locus_tag="PK768_05215"
     CDS             complement(1691..1936)
                     /locus_tag="PK768_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60403.1"
                     /translation="MTNNKRGFSKIDASAEEIQDKFGVDLYFFLDAVSLGLSDAEIAD
                     LTGYDLQKVRKVREHLGNVSSKIGISYKKNLPKPDHS"
     gene            complement(1949..2653)
                     /locus_tag="PK768_05220"
     CDS             complement(1949..2653)
                     /locus_tag="PK768_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HQA60404.1"
                     /translation="MSLLKDLFMHAYKAVILFTVSLVLVRFMGKRTVAQLSPFDLIFM
                     IVLGSAIAIPLEERDISLVLGVFPVVITALLNYLLAILITKNRKIENFIQGTSRVLVR
                     KGEVILGNLKKERITMTDLLILLREKGVTDINEVEEATIEPNGKLSVIKKEYMQTVTP
                     KDLGLWSKQGIFPTLMIHQGEVVFDNLDRVGVSIGQMMGQLNQKGITRLREISAAWID
                     EEGNLSIKLKNDEIIQ"
     gene            complement(2650..3072)
                     /locus_tag="PK768_05225"
     CDS             complement(2650..3072)
                     /locus_tag="PK768_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60405.1"
                     /translation="MQSPTKIEYAPHMLTYKENLKQLTESIKDLESQLGENLQQALTK
                     SSSLVRLNKGIRELELLSQAMADVNPDKNTKAREELVASFRRKLFENQCLLLDEIQKS
                     MLKAAEAMINAGSGITMLASFIQMNRVLENFDNRVKGI"
     gene            3252..4493
                     /locus_tag="PK768_05230"
     CDS             3252..4493
                     /locus_tag="PK768_05230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQA60406.1"
                     /translation="MKQYIYTKLVYIVAIFCLFSFSTSTALADTDKMQLPPEIVGQAG
                     LVMDVLTGKVLYEKNAHSPFEPASTTKIMTAILALEKGNLSDIVVTSEEPPRVDGSRI
                     YLEEGEKLTLEQMLYGMMLNSGNDAAVAIAEHIGGDIESFVKMMNAKAREIGAYHTTF
                     VNPSGLPDEGHLTTAYDLALISRYALLNLPEFRKIVSTKTLEIPWQAEEWDRQLINLN
                     KLLWNYEGADGVKTGYTSTAGRTLVASATRDGWQLISVVLKSDANIWSDSMALLDYGF
                     ENFEIKNLVGKNSDVAEEDVKYGDPVKLVTKDGLTSVVKKNDLPISKKLVINQDIKAP
                     VSKGEVLGQLIFYQGEEQLGSVNLIAANDVKRKIHTFWWFWALTIILATYIPFRIMVG
                     IKRYKRHKRRIQYVTYMNRYR"
     gene            complement(4606..5565)
                     /locus_tag="PK768_05235"
     CDS             complement(4606..5565)
                     /locus_tag="PK768_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HQA60407.1"
                     /translation="MFSFKARKWAVISGVVAVFILGASIYFTLEKEVIIRDEDKEAVV
                     STFASNVAELLEKQNITLDPEDEVTPSPDTKLSEGMQITIKRAFPVKIAVDGKEITLK
                     TRPKTVANILAKAKINLDEKDKVQPALGEYILGEAEINVIRVNERVTTEIRSIPFETV
                     SRKDYNLPLGEKKVIQDGEEGQEEVTTIEILENGKVVSTTTESKVIKAPKPKIVLTGT
                     VQVASRGGVDFSYIEKKRMLATAYTHTGNRTATGTMPRVGVVAVDPKVIPLGTRLYID
                     GYGFARAEDTGGAIKGDKIDLFLDTSEETKRFGRRWVTVYILK"
BASE COUNT         1585 a         1224 c         1073 g         1873 t
ORIGIN      
        1 atacctggac atttatatcc ttatcaactg cgataatagc attaggtgtt gactgtatga
       61 tcaggttaga gagggattcg gctcgatttc tcatataggg caggcacatg tgaatttcag
      121 ccatgccgtt atacacagcc tccgcctttt cccgacatga attgtagcca caagcaccgc
      181 agtttaattc atgttcaggc ctatacttcc ccgtagcctc caaaatgcga gtaatgtcct
      241 gttcgctggg tttttttgtt tttatgggag taggctcaaa ctttttttcg aaatcaaaag
      301 acaagccctg accggatatg gaaatagact ccttgcaagg gctgttatgt cgcgcaaaaa
      361 tttttacaca cttaattcgt ttaaagggac ctctatctat gatatcaata gcaggcccat
      421 ttatacagcc gccttcgcag gcattcattt ctatccaggt gcgatgaaac tcgccttttc
      481 tcaattcatc cagcataagc atgcagtttt ccttgccgct aacttcaata aactcccgcc
      541 aggggtcttc gattagtggt tttatggtct tgtaactgcc cccgggaata ggataaaaat
      601 tggcatcgtc acaaaagcca ttatcctgtt catttacggg ttctagtata ggatctatcc
      661 cttcttcctt ccaccaggct gtgagttcct caaaagtcaa ggccgcatcg atgccgtaat
      721 tgccattgtc atgaatttct acttttttcg cgaggcaagg ccctataaat gctacttcct
      781 tgtgtccctt tatctttttt agatatctgc catgggcttc catgggagat accactggga
      841 ctaaacattc tactagttcc gggtagtatt tttgaaccag gtaattgatg gcgggacatg
      901 ccgttgtaat cagattgggt tttgttctgt cgttatagtg ctttaaatag tctgaggcaa
      961 ttagtcttgc gccaaccgat gtttgataaa catttgaaaa accaagtctt tttatggctg
     1021 atacgatctg actataatga ttaaaatgaa aaaccccggc aaaagagggc gcaaggctta
     1081 cggcaatatc cttgtctatc tttagtaact cttttactcg ctgaatatcg ctttttatgg
     1141 tcttggcatt ttgtggacaa attgtaagac atgtaccaca ggtaatacat ctttcgtcaa
     1201 ctatctgggc ttggtcgttt tccatgcgta tagcctttac agggcacgct cttaagcatt
     1261 tatagcaatt tttacaattt gcactggcaa aattaatgac ctccattata ttaagccccc
     1321 aatatcttct ccttgaaaaa ggcctccgca ctttccaggt caacagaata gggttcgctg
     1381 tcatctactc tcaccgagac aggattgcga caattgttgc agcaaaactc tgcttttaac
     1441 tcccacttat cttccagacc gtgttcctta atcaaggcct taaatattct tataatatca
     1501 taggaacctt ttaagtgaca ggagctgcca acacatacgc tgattgttct catagtattt
     1561 cccctctcct caattgaata ttccattaaa aaagagtctg catttcgctc taaagacatt
     1621 atataaataa aaaagggtat tgtcaaatat ttcacaatcc cgcgtacagg ttaccttctt
     1681 ttggataaga ttaagaatgg tctggctttg gaaggttttt cttgtagctt atccctattt
     1741 tactgctgac gtttcccagg tgttccctta cctttctcac cttttgtaaa tcgtagccgg
     1801 tcaggtctgc tatttcagca tcgcttagcc ccaatgaaac tgcatctaaa aaaaaatata
     1861 aatctactcc aaatttatct tgtatttctt cggcactggc atctattttg ctgaagcctc
     1921 ttttgttatt tgtcatctgc tcaccaactc attgtataat ctcatcattc ttaagtttaa
     1981 tagacaggtt tccctcttcg tcaatccatg cagcactgat ctctctcagt cttgttattc
     2041 ctttttgatt taattgtccc atcatttgcc ctatactaac tccaacccta tccagattgt
     2101 caaaaacaac ttccccctga tgtatcatta atgtagggaa aattccctgc tttgaccata
     2161 gacccaaatc tttcggagtc accgtttgca tgtattcctt ctttattacg cttaacttcc
     2221 catttggttc tattgtggct tcctccactt cattgatatc cgttacccct ttttctctta
     2281 ataaaataag caagtcggtc atggttattc gctctttttt cagatttccc aatatgactt
     2341 ctccttttct cactagaacc cgagatgtgc cctgtatgaa attttctatc ttgcgatttt
     2401 tggtaattaa tatggcaagc aaatagttta acaatgctgt aatgacaacc ggaaagactc
     2461 ccagtacaag gcttatatca cgttcttcca ggggtattgc tattgccgaa cccaatacaa
     2521 tcataaaaat tagatcgaag ggtgataatt gggcaactgt tcgctttccc atgaacctta
     2581 ccaacacaag actcacagta aaaagtataa ctgccttgta agcatgcata aacaaatctt
     2641 tgagcaaact catattcctt tcaccctatt atcaaagttt tcgaggaccc tgttcatctg
     2701 tatgaaactt gccaacatcg ttataccgct gcctgcattt atcatagctt ctgccgcctt
     2761 cagcatggac ttttggattt catcaagaag caaacactga ttttcaaaga gttttctgcg
     2821 aaaggaagcc actagttctt ccctagcttt tgtgtttttg tcaggattga catctgccat
     2881 tgcttggctt aaaagttcaa gttctcttat acccttattt aatcttacaa ggcttgatga
     2941 ttttgtaagg gcttgctgaa gattttcccc aagctggctt tcaagatctt tgatggactc
     3001 tgtcagttgc tttaagtttt ctttatatgt aagcatatga ggtgcatact ctattttagt
     3061 gggactttgc aattttttcc actccatttc ctgtatttat tttaaggaat atgacagttt
     3121 ttattcacca ccgccttttt caagaaaaat acagttggaa tagactttga gatttgatat
     3181 acttattttt agtatgaaac taaacggagt caaaaatcaa aattaaaggt gcagatttac
     3241 ggggtgaata tatgaaacaa tacatataca caaaattagt atacatagtt gcaatctttt
     3301 gtctatttag tttttcaact tctacggcgc tggctgatac tgataagatg cagctgcctc
     3361 cggaaatcgt cggccaagca ggtttagtta tggatgtttt gacgggaaag gttctatatg
     3421 aaaagaatgc tcacagtcca tttgaaccgg caagtacgac aaaaataatg actgcaattt
     3481 tggcccttga aaaaggcaat ctttcggata tagtcgttac aagcgaagaa ccgccacgtg
     3541 ttgatggctc taggatttac ctggaggaag gggaaaagct gactttagaa caaatgcttt
     3601 acggtatgat gctaaattcc gggaacgacg ctgctgttgc catagcagaa catatcgggg
     3661 gagatattga aagcttcgtg aaaatgatga acgcaaaagc aagggaaatt ggcgcatacc
     3721 acaccacctt tgttaatcca agcggcttgc ctgacgaagg ccatttaaca actgcctatg
     3781 acttggctct catttcacgt tacgcccttt taaaccttcc tgaattcagg aaaatagtct
     3841 ctactaaaac attggagata ccctggcagg ccgaggaatg ggacaggcag ctgataaatt
     3901 taaataagct tctttggaac tacgaaggag cagatggtgt caagacaggt tatacgagca
     3961 ctgccggacg cactttggta gcatcagcaa ccagagacgg ttggcaactg atttcagtag
     4021 tgttaaaatc cgatgcaaac atctggtcag acagtatggc gctgctggac tatggctttg
     4081 aaaacttcga aataaagaac ctggttggta aaaattcaga tgtcgcggaa gaagatgtta
     4141 aatacggcga tccggttaaa cttgttacaa aggatggatt aacttccgta gtgaagaaaa
     4201 atgacctgcc gattagcaaa aagcttgtca taaatcagga tattaaggca cctgtttcga
     4261 aaggtgaagt cctgggtcaa ttgatatttt accaaggtga agagcagctg ggaagcgtca
     4321 atttaattgc tgctaatgat gtgaagagaa aaattcatac cttttggtgg ttctgggcac
     4381 tgactattat cttggctact tatattccct tcaggataat ggtaggaatc aaaaggtata
     4441 agaggcacaa gcgaagaatt caatacgtca cttatatgaa tagatacaga tgataattat
     4501 atatataaag agagcactgc aattagttcg cagtgctcat ttttgtgcgt tttgcaaaag
     4561 gtacgttggc aaaacgaatc acagtttgta tagttgggcg ggttcttatt ttaagatata
     4621 tacagtaacc catcttcttc cgaaacgctt agtttcctcg gaagtgtcta aaaacaagtc
     4681 tattttatca ccttttatgg caccaccggt atcctcggct cgggcaaagc cgtaaccatc
     4741 tatatacagc cttgtcccta aaggaataac ctttgggtca acagcaacaa cacctactct
     4801 aggcatcgtt ccagtcgcag tgcgattgcc tgtatgagtg taagctgttg ctagcatgcg
     4861 ttttttctcg atatacgaaa aatctacacc tccacgtgat gcaacttgta ccgttcctgt
     4921 cagtacaatc ttaggctttg gagcttttat gacttttgac tcggtagttg ttgagacaac
     4981 cttgccattt tcaagtattt cgatcgtagt tacttcttcc tgaccttctt ctccatcttg
     5041 gattactttc ttttcaccaa gcggaaggtt atagtctttt ctggaaacgg tttcaaaggg
     5101 tatgctccta atttcagttg taactcgttc atttactcta attacattga tttcagcttc
     5161 acctaatatg tattctccaa gagccggctg aaccttgtct ttttcatcca gattaatttt
     5221 agcctttgcc aggatatttg ctactgtttt tggccgggtc ttaagggtga tttccttccc
     5281 atcaactgct atctttacag ggaatgctct ttttatggta atctgcatac cttctgaaag
     5341 cttggtgtca ggacttggag taacttcatc ctcagggtct aaagttatat tttgtttttc
     5401 caacagctcc gctacattac tggcaaaagt tgaaactaca gcttccttgt cttcgtctct
     5461 aataatcacc tctttttcca aagtgaaata gatagacgct ccgagaatga atacagccac
     5521 caccccggag ataacagccc atttacgggc tttaaacgaa aacacaaaac tccccctttg
     5581 taaaaggtct attaaggatt tctactttta ttaatgaaat cctttttcca gttttttctc
     5641 tatctgtttt tattatatta tacttatacc aaaactatac atggcttgtc aaatttagta
     5701 caaataacac tattttttat tgttgccact tattgagggt tttatgatat cattg
//
LOCUS       DAONUO010000111         5749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10340_length_5749_cov_5.096417, whole genome shotgun sequence.
ACCESSION   DAONUO010000111 DAONUO010000000
VERSION     DAONUO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10340_length_5749_cov_5.096417"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..441
                     /locus_tag="PK768_05240"
                     /pseudo
     CDS             187..441
                     /locus_tag="PK768_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     regulatory      572..754
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            898..3234
                     /locus_tag="PK768_05245"
     CDS             898..3234
                     /locus_tag="PK768_05245"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HQA60408.1"
                     /translation="MITKILKRDGREVPFNNEKITNAIYKATIAAGEEDYEKAMALAE
                     KVVEKLHKDPNIQVPSVEDIQDIVERVLIEEGLTQTAKKYILYRAERTRIREMKTRLM
                     KTYEDITFKESKDNDLKRENANIDGDTAMGTMLKYGSEGAKQFCEMFLLKPEHAEAHK
                     NGDIHIHDLDFYILTTTCCQIDIVKLFKNGFSTGHGYLREPNDIQSYSALACIAIQSN
                     QNDQHGGQSIPNFDYGMALGVAKTYSRLYRQNLAKGLELLLDMKDATTLVKMVSKKLE
                     DEDGLYPTLIPNERYLDKERKHLLEFIPDVKLINKVQSFAIEKAQAETDRNTYQAMEA
                     FIHNLNTMHSRAGAQVPFSSINYGTDISPEGRLVIKNLLLATEAGLGNGETPIFPVQI
                     FKVKEGINYNAGDPNYDMFKLACRVSAKRMFPNFAFLDAPFNLKYYRPGRPETEVAYM
                     GCRTRVIGNVYDPSREIVYGRGNLSFTTINLPRIALRSNNDISKFFTELDEKIDLVID
                     QLLERFEIQARKKVKNFPFLMGQGVWIDSDKLGWEDEVREVLKHGTLSVGFIGLAECL
                     KALTGKHHGESEESQKLGLKIIGHMRKRMDEASQKCKMNFTLLATPAEGLAGRFVKMD
                     RKIYGSIEGVTDREYYTNSFHVPVYYEINAFDKIRIEAPYHELTNAGHITYIEVDGDP
                     KQNLKAFEKIIRAMKEAGIGYGAVNHPIDRDPVCGYTGIIGNTCPYCGREEGEIKFER
                     IRRITGYLVGTLDRFNDAKREEERDRVKHMSFKSQNMR"
     gene            3241..3747
                     /gene="nrdG"
                     /locus_tag="PK768_05250"
     CDS             3241..3747
                     /gene="nrdG"
                     /locus_tag="PK768_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016092073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HQA60409.1"
                     /translation="MDTYIKIAGIIKESIVDGPGIRMVVFAQGCKHNCPGCHNPETHS
                     FDGGTLVTVDSIIEQAKRNPLLDGITFSGGEPFEQAQAFAVLARESKKLHLNVMTYTG
                     YTYEYIISNSSRHKGWEELLNETDILVDGRFELNKRNLLLKFRGSENQRIIDVKRSKS
                     ENRIVIMD"
     gene            4495..4710
                     /locus_tag="PK768_05255"
     CDS             4495..4710
                     /locus_tag="PK768_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA60410.1"
                     /translation="MKNRLEEIRKQKGIRQEKLAADLEVSRQTISSLENGRYNPSIIL
                     AFKIARYFGMSIEDIFIYEEGQENETK"
     gene            4697..5134
                     /locus_tag="PK768_05260"
     CDS             4697..5134
                     /locus_tag="PK768_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60411.1"
                     /translation="MKQNKIVRYIVFAIIGFIIFGIGLFLIIQLPDADGILKTLPYIC
                     IGLGSGLFGGSLGTAIKNWFVLKNPEIAKQVEIEEKDERNRSISDRAKAKAYDLMLYT
                     YFIILLAFALMGVELYVEITLVVVYLFIISTKVYYLIKYHKEM"
BASE COUNT         1958 a          884 c         1263 g         1644 t
ORIGIN      
        1 agtgattgta tatgatgata ggttagtggt tgagtttaag tctgggttag aaattaatgt
       61 gcagccatag ttataaacta tgactaagaa taaaatatca gatattactt atggctacga
      121 ttttagtgat agtatttttt aagtagacta agatgaaagg aagaaagttt caaaagtgaa
      181 gaagagacag aaaaattgta tcttcatctg cgtgatgaag gatatattaa agtacagggg
      241 gatgtaagta acagaattca ggtcgatagt atgatgaata taatatataa tcgtttcggc
      301 gatattgctg ttctagtaaa taatgccgga gttgcacagc aaaactattt actgatatat
      361 ctgagaatga ttgggattga atgtttaatg ttaatattaa agggatattt aattgctgcc
      421 aagctgtatt accaaagatg ataaataata agcggggaga aataattaat atttcgtagc
      481 cctaatggtg gatttgtggt ttagatatta gaatcataga ttcaataaaa tagattgaca
      541 caatatattg tatgtgataa tataatcaca actaaatatt gacattgcag gtgactgtta
      601 ttgacagttt aaaagggaag caggtgaaat tcctgcacgg tcccgccgct gtgatgggga
      661 gatgttccat gataatccac tggatgaatt ctgggaagga atggagtgtc gatgaaccag
      721 agtcagaaga cctgcctgta atgccacacc gtgaactcta cgagcgatag aggggtgtag
      781 gaatatctgc agtactctaa ttgtcactga aaacgagtat tgtgacgcct cttgacgctt
      841 gtcgggaggc gttagttatt atatttactt aaaatcacat agaaaagggg acgaaagatg
      901 attacaaaaa tcttgaaaag agacggaagg gaagtaccct tcaacaatga gaagataact
      961 aatgccatct acaaggcaac tatagcagca ggagaggaag actatgagaa agccatggct
     1021 ttggcagaga aagttgtgga gaagctgcat aaagacccaa atattcaggt tccgtctgtt
     1081 gaggatatac aggacatcgt cgaaagagtt ttgattgaag aagggcttac ccaaacggca
     1141 aaaaaataca tactgtaccg ggctgaacgg acacgcatca gggaaatgaa gacacggtta
     1201 atgaaaacat atgaggatat aactttcaaa gagtccaaag ataacgattt gaaacgggaa
     1261 aatgcgaaca ttgatggtga tactgctatg ggaaccatgc ttaaatatgg ttctgagggt
     1321 gcaaagcagt tttgtgaaat gtttttattg aagccagagc acgctgaagc tcataaaaat
     1381 ggagatattc acatacatga tttagacttt tatatcctta ctaccacctg ctgccagatt
     1441 gatatagtta aacttttcaa aaatggtttc agtacagggc atgggtatct gagagaacca
     1501 aacgatatcc aaagctattc cgccctggca tgtatagcaa ttcagtctaa tcagaacgat
     1561 cagcatggtg ggcaaagcat acctaacttt gattatggta tggctttagg tgttgcaaag
     1621 acctattcca gactatacag gcagaatttg gccaaaggat tggagttatt attggacatg
     1681 aaagatgcga caacgctggt taaaatggtt tcaaaaaaac ttgaagatga ggatggactt
     1741 tatccgacgc tgattcccaa tgaaagatat ttggataagg aacgaaagca tcttttagaa
     1801 tttataccgg atgtgaaact tatcaacaag gtgcagtctt ttgctattga aaaggcacag
     1861 gcggaaacag acaggaacac ataccaggct atggaggctt ttattcataa tctaaatacc
     1921 atgcatagca gagccggggc acaagtgcct ttcagttcaa taaattatgg tactgatatt
     1981 tcccccgagg gcaggctggt tatcaaaaac cttcttctgg ccactgaagc aggtcttggc
     2041 aatggagaaa cccccatatt ccctgtacaa atatttaagg ttaaagaagg cattaattat
     2101 aatgccggag atccaaacta tgatatgttc aagcttgcct gcagagtaag cgccaaaaga
     2161 atgttcccga actttgcatt tctggatgct ccgttcaatc tcaaatatta cagaccgggt
     2221 cgacctgaaa cagaggtagc atatatgggc tgcaggacaa gagtaatagg caatgtctat
     2281 gacccttcca gggaaatagt ctacggaaga ggaaatttga gctttacaac aataaatctt
     2341 ccaaggattg cattaagaag taataatgac ataagtaaat tctttactga gctggatgaa
     2401 aaaattgatc ttgttataga ccagttgctg gaaagatttg aaattcaggc ccgcaaaaaa
     2461 gtcaagaact tcccattctt gatggggcaa ggggtatgga ttgattcgga caaacttggc
     2521 tgggaagatg aagtcagaga agtattgaag catggtacgc tctcagttgg atttattggg
     2581 cttgcagaat gccttaaggc attgacagga aagcatcatg gagaatctga agagtcccag
     2641 aaacttggtc taaaaataat tggtcatatg cgtaaacgta tggacgaggc tagccaaaaa
     2701 tgtaaaatga acttcaccct tttggcaact cctgctgaag gactggcagg aagatttgtg
     2761 aaaatggaca gaaaaatata tggttccata gaaggtgtta ctgacaggga atattacacg
     2821 aacagcttcc atgttccggt atactacgaa attaatgctt ttgataagat aagaattgaa
     2881 gcaccatacc atgagctgac caatgcagga catattactt atattgaggt ggatggtgat
     2941 ccaaagcaaa acctgaaggc ttttgagaaa attatcagag cgatgaagga agccggcata
     3001 ggctatggtg ctgttaacca tccgatagac agagatcctg tatgcggata taccggaatt
     3061 attggcaata catgtcctta ctgcggtcgt gaagaaggag aaatcaaatt tgaaaggata
     3121 aggcggatta ccggatacct ggtaggtact cttgacagat tcaatgatgc caaaagggaa
     3181 gaggaacgtg acagggtaaa acatatgtct ttcaaaagtc aaaacatgag gtgaattaat
     3241 atggatacct acataaaaat agcaggaatt attaaagaat ccatagtgga cggaccggga
     3301 atacgaatgg tggtctttgc ccaggggtgt aaacacaact gccctggctg ccacaacccg
     3361 gaaactcatt cttttgatgg aggtacatta gtaacagttg attcaataat cgagcaagct
     3421 aaaaggaatc ctctgctgga cggtattaca tttagcggag gtgaaccttt tgaacaggcc
     3481 caggcatttg ccgttcttgc ccgagaatca aaaaagcttc accttaatgt aatgacttac
     3541 accggctata catatgaata tatcattagc aattcatcca gacataaggg ctgggaggaa
     3601 ttgctgaatg aaaccgatat acttgtggat ggcaggtttg aattaaataa acgaaatctt
     3661 ctcctcaaat tccgtggttc tgaaaatcaa aggattatag atgttaaaag gtcaaaatct
     3721 gaaaatagaa ttgttattat ggattaaact ataggatatg ggttaacatt taaacattaa
     3781 aaaccgactc cctattaggg gcattagata ctgttcgaga tattgccgaa gtgttttgat
     3841 tctttcatca agtaagcaag gaagatttta taacgtaaca gataattagc cctaacagtg
     3901 gaaatgttat ttagatattt tatataattc acattttaat tattgatatg ttcccgcgaa
     3961 cggtgctttc tcaaaaatga ccgaaaaaac gattgatatg tttcctcgaa cagatactac
     4021 aaaaaatcac ccatagacat caattccatc aactcgtccc atgttgagtg tgtagtttta
     4081 atgtcaaggg taaaagagtg aatgtgtgaa aaagcctcat aaatcaatac ttttagcata
     4141 catgattatg aaatccattg ggcgaaaacg agaatttttc acttcgcagt aacaagtcca
     4201 taaaggctgt atttgatagt taagtggtca ggttagatat cagccttcgc gagtggtcga
     4261 ttttagatgt tttttcagct tttttgaggt tgtattgtca ggttggatgt ggccgtgaaa
     4321 tagactgcta ttgcgacgat aaaacgtaat ttgaatggtt gatcatttat attcttcata
     4381 acagacttga aaaagtctgt tatttttttt ggaaagtata cttgacattt aatttaatcc
     4441 atgttatact ccaaatgtaa agcatgcttt ccatttgttg cagaaggtga tgctatgaaa
     4501 aacagactgg aagagatccg caaacaaaaa ggaatacggc aagaaaaact tgccgctgat
     4561 ttagaagtat ctcggcaaac gattagctct ttagaaaatg gacggtacaa cccatctatc
     4621 attttagctt ttaaaattgc aagatacttt ggtatgagca ttgaagatat ttttatttat
     4681 gaggagggtc aggaaaatga aacaaaataa aattgtgcgt tacatcgtct ttgctataat
     4741 cggttttata atcttcggga taggattgtt tttgattata cagttgcctg atgcagatgg
     4801 catactcaaa acattgccat atatttgcat aggtttgggt tcgggtttgt ttggtggtag
     4861 tcttggaacg gctattaaaa attggtttgt acttaaaaat ccggaaattg caaagcaagt
     4921 tgaaattgag gagaaagatg agcgtaatcg ttcaattagc gatagggcaa aagcaaaagc
     4981 ctatgattta atgctgtaca cctatttcat aatattgttg gcttttgctc tgatgggagt
     5041 agagttgtac gttgaaatta ctcttgtagt agtttattta tttatcattt ctacgaaagt
     5101 ttactacttg attaaatacc ataaagagat gtaaaacaca tggatatgtt tccactattt
     5161 tactggattt gctggattac attcagtctg cctactcaac ctaaagggcg aaaatacgta
     5221 catggaaaca tattgaggga gtattttggg gttatatttg agcccaaaaa ttagtagggt
     5281 tgagttgaca gattagataa tgggcatctt tcataagatt gaggagacag gatggatgta
     5341 cattctatgc aagcttaata tctctcaaag atagaataac aaagcctcat ttataaataa
     5401 aattgactta aaaaatctcg gttgatataa ttttcctaaa atattatttg aagtaatata
     5461 tgggtaataa tattgagata cgaaaactgt cacctgaact tgcagaagat tatgttcatt
     5521 tttttgatat aacaccgcat tccgaaatac ctgattctga tgactgcaaa tgttattgtg
     5581 tatggtgatg taatgacgat caagatgttg atcatattga ttatttatta tcaaaagaaa
     5641 aaagaagaga ttgtgctatc caaaaaaata agagaaaaca aaatacaggg ttaccttgct
     5701 tagcataatg gaaaggatgt aggatggtgc aataccaaca ccaaagctg
//
LOCUS       DAONUO010000112         5645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10566_length_5645_cov_3.709302, whole genome shotgun sequence.
ACCESSION   DAONUO010000112 DAONUO010000000
VERSION     DAONUO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10566_length_5645_cov_3.709302"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..955)
                     /locus_tag="PK768_05265"
     CDS             complement(20..955)
                     /locus_tag="PK768_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HQA60412.1"
                     /translation="MNPKIVCLGGGTGLPNLLRGLKLYTDNLTAIVTVADDGGSSGIL
                     RDELHIPAPGDIRNCLCALACTEPLMEELFQYRFSCGSLKGHSFGNLLLAAMTEILGN
                     FELAIKESSKVLAVKGRVLPSTSENVVLEAVYDDGEIACGESHIPNSRKRISRVMLKP
                     SDAKPSEEAIEAIKQADAIILSPGSLYTSLIPNLLVKDISSAISESKARKIYVVNVMT
                     QPGETDGYKASDHVRAVIKHSCSAVMDYVIINSGQIPDYLLSRYLQDGSKPVECDSEV
                     IEAMGYKTVTAPVINPTDVVRHSPELLAEVVMKII"
     gene            complement(960..1832)
                     /gene="rapZ"
                     /locus_tag="PK768_05270"
     CDS             complement(960..1832)
                     /gene="rapZ"
                     /locus_tag="PK768_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQA60413.1"
                     /translation="MKDLKLVIITGLSGAGKTLAMRVFEDHGYFCVDNLPPALIPTFM
                     ELCSHSLKKINKIALVIDIRGGGFFDKLFESLNEMADSGYDYEILFLEASDEVLIKRY
                     KESRRRHPLALEGRIVEGIASERQIMSRLRDEADYIIDTSNKKPSELKEEITMRFIEQ
                     GREERLLINLVSFGFKHGIPLDADLIFDVRFIPNPYYIDELRPCSGKDKEVKDFVMNW
                     PESRQFLKKLLDMVQYLIPFYIREGKTQLVIAIGCTGGRHRSVVITNELAGMLKANRH
                     RVTVDHRDIDKDRG"
     gene            2067..2411
                     /locus_tag="PK768_05275"
     CDS             2067..2411
                     /locus_tag="PK768_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HQA60414.1"
                     /translation="MNVYDAAHQLARRLRECPEYIEFKRAKESLKEDPQAENMLKDLR
                     NKQFELQALKLSGKPTDEAEENLRNLYNIVAHNSLLKRYLEAEERFAVLFSDIQDIIM
                     KGIELDIDLNKK"
     gene            complement(2474..3814)
                     /gene="mgtE"
                     /locus_tag="PK768_05280"
     CDS             complement(2474..3814)
                     /gene="mgtE"
                     /locus_tag="PK768_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQA60415.1"
                     /translation="MNEREQILGLLEDKNYEELYPILEQYHPVDIAQLMEELSENESI
                     NLFSALEFDTALQVLEEIDSRKKYLILTSVEYNYARKLIQEMSADEVADLLGDLKEEE
                     KRQILSLLEETEQTDLIDLLSYERNTAGGRMTTEYIAFPKELKAKDALNRLAELAIDA
                     ETIYYVYVVDSQGRLVGVVSLRDLILAPEDTCIKDFMQTDVKKVNALQEQEEVAKLIQ
                     KYGLLALPVVDDDNVLLGIVTVDDAMIVVEEETTEDILKLAGSNVDESIDLGQVSIWA
                     RAKRRLPWILIAIAGEIVSGRVINDFSAALQTIVTLSLFIPVIMDMGGNVGTQSAAIM
                     VRGLATGELESLSIAKNVLREAAVGIILGLLNGILVAAITYLWQGQVLLGLVVGIAMA
                     FNLTLAAMLGTFFPLLWYKLGQDPAVASGPLVTTFLDILGLLVYFSTATWILGL"
     gene            complement(3811..>4710)
                     /gene="mgtE"
                     /locus_tag="PK768_05285"
     CDS             complement(3811..>4710)
                     /gene="mgtE"
                     /locus_tag="PK768_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQA60416.1"
                     /translation="ETIYYIYVVDKNKHLEGVLSLRELITAPRDKKIHEIMHTNIVSV
                     HVDQDQEEVAKIISKYSLLAVPVVNDSNRLLGIVTVDDAMEIIEEEDTEDIHRMAGIT
                     PEEDVAFSKTIWGSSKNRLLWLVICLIGNMFSGKVIDGFSHVLESMVALAFFIPVLMA
                     TGGNVGTQSLALTVRSMATEKLSRKNVLIFILGETLAGLQLGIICGGIVSVLAYLWQS
                     NIQLSMAVGLSMGISLILASLIGVLVPLIFHIFDIDPAVASGPLITTLVDISTLAIYF
                     TVSTYLIDVISPDLVSTAKGVLA"
     assembly_gap    4711..4720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4721..5197)
                     /locus_tag="PK768_05290"
     CDS             complement(<4721..5197)
                     /locus_tag="PK768_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQA60417.1"
                     /translation="MLPKKDVVLIKHFVLNKSDEAHKILANLHPADIAQILSEIKNGN
                     KKLFFQMMTSSKAAEVLEEIEPHDRISIIDELTEAETIKILENMSVDEIIDLFQQMPS
                     AQAESLLRKLPEEDYEELKSLLRMAEDTAGGLMTTDYVYIFSDATVNEAISDVRQFG"
     regulatory      complement(5370..5535)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
BASE COUNT         1538 a         1327 c         1129 g         1641 t
ORIGIN      
        1 aaaaaacctt caatctaagc taaattatct tcatcacaac ttctgccaga agctcagggc
       61 tgtgacgaac tacgtcggtg gggtttatta ccggagctgt cacagtcttg tatcccattg
      121 cctcaatgac ttcactgtca cactcaacag gcttagagcc atcttgtagg taacgcgata
      181 agagatagtc gggtatttgc cctgaattaa taattacata atccatcacg gctgaacatg
      241 agtgtttaat aacagccctg acatggtctg aagccttata accatcggtc tcgcccggct
      301 gtgtcatcac atttaccaca taaatctttc ttgccttcga ttctgatatg gcagagctta
      361 tgtcctttac gagcagattt ggaattaagc ttgtgtaaag gcttcccggg ctaagtataa
      421 tggcatctgc ctgttttatg gcttctatag cctcctccga gggttttgcg tcagaaggct
      481 tcagcatcac cctggagatt cttttcctgg aatttggaat gtgagactct ccacaagcta
      541 tctcaccatc atcgtataca gcttctaaaa ctacattttc cgaggtagaa ggaagaaccc
      601 ttcccttgac ggccagtacc ttgctggact ccttaatggc aagttcgaag ttgcctaaga
      661 tttccgtcat cgctgccaac aatagatttc caaagctgtg gccttttaag ctgccgcagc
      721 taaagcggta ttggaataat tcctccatta aaggttcggt acaggctaga gcgcataggc
      781 aattcctgat atcgccgggt gcaggtatat gaagttcgtc ccttagtatg cctgagctgc
      841 cgccgtcatc ggcaacagtt acaatggcgg ttaggttatc cgtataaagc tttaaacctc
      901 tcaaaagatt ggggagcccg gtgcctcctc ccaaacaaac tattttcgga ttcatatttt
      961 caaccccgat ccttgtctat gtctctgtgg tccaccgtaa ctctgtggcg gttagcttta
     1021 agcattccag ccagttcatt ggttatgacc acggagcgat gcctgcctcc ggtgcaacca
     1081 atagcgatca ccaattgagt tttgccttcc cgaatataaa agggtattag atactgcacc
     1141 atatcaagca gtttttttag gaattgcctg ctttcgggcc aattcatcac aaagtctttt
     1201 acctctttgt ctttgcccga gcaaggccgc agttcatcga tatagtaagg gttgggtata
     1261 aacctgacat caaagatcaa atcagcgtct aatggaatcc catgtttaaa accgaaggac
     1321 accaggttta ttaaaagtcg ttcttccctt ccctgctcga tgaatctcat tgttatttct
     1381 tctttaagtt cggagggttt tttattggaa gtgtcaatga tataatcggc ctcgtcgcgc
     1441 agccttgaca tgatttgccg ttcggaagct atcccctcca caatgcggcc ttccaatgcc
     1501 aagggatggc gccgccgact ttccttatag cgctttatga ggacttcatc cgaagcttcc
     1561 aaaaacagga tttcgtaatc atagcccgag tcggccattt cgtttaaact ttcaaacagc
     1621 ttatcaaaaa aacctccacc cctaatgtct ataaccaatg ctatcttgtt tatcttcttc
     1681 aagctatgac tgcagagttc cataaaagtt gggattaaag caggtggaag attgtcaaca
     1741 caaaaatagc catgatcctc aaaaactctc atcgccaaag tcttgcccgc acccgaaagg
     1801 ccggttatta tcactagctt caaatccttc atatgtctca ccttttgttt acgctaaact
     1861 ttctatgctg atatttggca ttggttatgt cgactgtacc gggcatgagt atgccattct
     1921 gtccagcttc atataagtcc tctctataac agtataatat aaattgaaac taaaatctat
     1981 gtaaaaaagc ttgtgttgga aaacattcaa tatttgttat aattgagtta gatagctaga
     2041 tttaaaacta aaggaggctg ctgaatatga atgtatatga tgctgcccat caacttgcaa
     2101 ggcgtttgcg cgagtgtcct gaatacatag agtttaagcg agccaaagaa agtttaaaag
     2161 aggatcccca ggccgaaaac atgctaaaag acctgaggaa taaacaattt gagctgcagg
     2221 ctcttaagct ttcaggaaag ccaaccgatg aagctgagga aaacttgagg aatctgtaca
     2281 acattgttgc ccacaattcg ctgctaaaaa ggtatcttga agcggaagaa cgctttgcag
     2341 tgctgttttc agatatccag gatatcatca tgaagggaat tgaactcgat attgatttaa
     2401 ataaaaaata gaatgtgctt ttaatcaata taccgccaaa ctctccgaag tttggcggtt
     2461 tgttttccac cggtcacagc cctaaaatcc atgtggctgt agaaaaataa actaagagtc
     2521 ccaagatatc aagaaaggtt gtcacaaggg gtcccgaagc cacggccggg tcttgaccaa
     2581 gtttatacca caagagggga aagaatgtgc ctagcattgc cgcaagtgtc aggttaaagg
     2641 ccatggcaat tcccaccaca aggcccagca acacttgccc ctgccagagg tatgttatgg
     2701 cagctaccaa aatgccgttc agcaggccca gaataattcc cacggcggct tcccgaagga
     2761 cattttttgc aatggacaag ctttccagtt caccggtggc aaggcccctt accatgatgg
     2821 cagcagattg tgtgccgaca ttgcctccca tgtccataat tacgggaata aatagggata
     2881 aagtcactat ggtctgcagg gcagcagaaa agtcgtttat gaccctgccg gatactattt
     2941 caccggcaat cgctattaaa atccagggca gccttcgctt tgcccgggcc caaatcgaaa
     3001 cctgacctag gtcaatgctt tcatcaacat tcgagcccgc taacttcagt atgtcttctg
     3061 tagtttcttc ttccactaca atcatggcat cgtctacggt cactatgcct aatagcacat
     3121 tgtcatcatc aactacgggc aacgccaata agccgtattt ttgaatgagt ttcgccactt
     3181 cttcttgttc ctgtagggca ttgacctttt ttacatcggt ctgcataaaa tctttaatac
     3241 aagtatcctc aggcgctagt attaaatccc tcagtgaaac tactcctacc agccttccct
     3301 gagaatccac cacatacaca taataaatgg tctccgcatc aatggccagt tccgccaatc
     3361 tattgagagc gtccttggcc tttaactctt tgggaaaggc tatatattcc gtggtcatcc
     3421 tgccaccggc agtgtttctc tcgtaagaca acaagtctat taagtcggtt tgttccgttt
     3481 cttccagcaa ggacaatatt tgtctttttt cttcttcctt taaatcgccc aataagtcag
     3541 ctacttcatc cgctgacatt tcttggatta gtttgcgcgc ataattgtat tcaacactcg
     3601 ttaagattaa atattttttg cgtgaatcta tttcttccaa aacctgaagg gcagtatcga
     3661 attctaatgc cgagaacaaa tttatacttt cattttccga aagctcttcc atgagctggg
     3721 cgatgtccac tggatgatat tgctcgagta tcggatataa ttcttcataa tttttatctt
     3781 ccaaaagtcc aagaatctgt tccctttcgt tcatgccagc accccctttg cggtgctgac
     3841 aagatccggt gaaatgacgt caatgagata agtagaaacg gtaaaatata ttgcaagggt
     3901 agatatgtcc acaagtgtag ttatgagagg gccggaagct acggcaggat caatgtcaaa
     3961 aatgtgaaat attaagggca ccaggacacc gataagggag gccagtatca aagatatgcc
     4021 catagagagc ccaacggcca tgctgagctg aatattgctt tgccaaaggt aagctagaac
     4081 tgatactatc cctccgcata ttattccaag ttgcagccct gctaaggttt cccccaatat
     4141 gaaaatcaat acgttcttcc tgctcaactt ttccgtggcc atgctcctga ccgtcagtgc
     4201 caatgattgg gtgcctacat tgccccccgt tgccatgaga accggaatga aaaaggccag
     4261 agcaaccatg gactccagta catgcgagaa tccatcaatg accttccccg agaacatgtt
     4321 tcctataagg catataacga gccacaagag tcgatttttc gaggaacccc aaatggtctt
     4381 tgaaaaagcc acatcttctt ccggcgtaat acctgccatt ctatgaatgt cttccgtatc
     4441 ttcctcttct ataatctcca ttgcatcgtc aaccgttacg atacccagga gccgatttga
     4501 atcgttaaca acaggaactg cgaggaggga atacttggat atgatttttg caacttcctc
     4561 ctgatcctgg tctacgtgaa ccgacacgat attcgtgtgc ataatttcat gaattttttt
     4621 gtccctagga gcagtaataa gttctcttaa agacaataca ccttccagat gcttgttttt
     4681 atctactacg taaatgtagt aaattgtctc nnnnnnnnnn gccgaactgt cgcacatctg
     4741 agatggcctc atttacggtg gcatccgaga atatatatac ataatctgtg gtcatgaggc
     4801 cgcctgccgt gtcttctgcc atcctaagga gcgatttgag ctcctcgtaa tcttcttcgg
     4861 gaagctttct caaaaggctt tcggcttgag ccgagggcat ttgctgaaaa aggtctataa
     4921 tttcatctac tgacatgttt tctaagatct ttatggtttc cgcttcagtc agctcatcaa
     4981 taatggaaat ccgatcatgg ggctcaattt cttccaacac ctcggctgcc ttagagcttg
     5041 tcatcatttg aaaaaatagc tttttattgc cgttctttat ttcactgagt atctgggcaa
     5101 tgtctgccgg gtgaaggttt gcaagtatct tgtgtgcttc gtcgctctta ttgagtacga
     5161 aatgctttat caaaactaca tctttttttg gcagcataac tgtccctcct ttttaagagg
     5221 tccatctacc caccacagca gcagcggtaa gatagacctc attgatatta agttttataa
     5281 agcatcgact aggatccggg tccatcgtac cgcccccttt gcatgataaa attaataaaa
     5341 gcccttttta gacgaaaggg catagtaata ctatttcccc tcgtctcggt tttagcactg
     5401 tacgactttg gaaccctgtt ccagccacat tagatttgcc ttaatccggc gaaacctgtt
     5461 aacccattgg tgtctctcga catttccggg cagtggcctg tattcgcaca ggagcctcac
     5521 ctaacgggaa gtaaaaacta tttttcactt acttttattt taatacttta atgaaatata
     5581 tgtcaagcta ggtctaaaaa atgataatgt caatttgttg taggataatt aaacttaaag
     5641 gtatc
//
LOCUS       DAONUO010000113         5630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10591_length_5630_cov_4.259372, whole genome shotgun sequence.
ACCESSION   DAONUO010000113 DAONUO010000000
VERSION     DAONUO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10591_length_5630_cov_4.259372"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK768_05295"
     CDS             <1..551
                     /locus_tag="PK768_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQA60418.1"
                     /translation="RKKVANNGLHYKKSQTSYNNKEFKKISPGTGFYKAERKLLKLII
                     ENESIRRNVGEKIDERYFTNDRTKKIADVLFKTIRQNTNQDIDVSNLFNYLDEESSAE
                     LSSILAERAELINEELICSLVTKVKEGYFKESIVKVREQLKQAEILGKREEINQLLNI
                     YQQLKAEMDELNSHTTPGKEGA"
     gene            552..1679
                     /gene="rpoD"
                     /locus_tag="PK768_05300"
     CDS             552..1679
                     /gene="rpoD"
                     /locus_tag="PK768_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HQA60419.1"
                     /translation="MANKEQPQKKADKKPLQDKMKAATDLIEKGKKTGKLTYEEIMDA
                     LDEIEDLDADQIDKIYESLEEMGIEVLDGSEIVVPGEEGLDDVSEEDLDLSVPEGVAI
                     DDPVRMYLKEIGKVPLLSAEEEVELSKRIEKGDKEAKRKLAEANLRLVVSIAKKYVGR
                     GMLFLDLIQEGNLGLMKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVH
                     MVETINKLIRVQRQLLQELGRDPLPEEIAEEMNLTVEKVREIMKIAQEPVSLATPIGE
                     EEDSFLGDFIPDDEALAPADAAAYLLLKEQLEDVLETLTPREEKVLRLRFGLDDGRPR
                     TLEEVGQVFGVTRERIRQIEAKALRKLRHPSRSKKLKDFLE"
     gene            1741..2424
                     /locus_tag="PK768_05305"
     CDS             1741..2424
                     /locus_tag="PK768_05305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQA60420.1"
                     /translation="MKLANRLKIIASMIEKGSTVVDIGSDHAYLPIYLVKEGISQRVI
                     ATEILSGPFERARENIKKSCYQDFIQLRYGSGLKPIKPGEVDVAVIAGMGANTMINII
                     EESRETVDSLKKLILQPMCNQARLRKHLFQRAYTIIDEDVAMDANKYYEIIVAQKASI
                     VAFDEIDILVGPVLRCKKTPVVEEYIHYRIKKLQKILETLEASNSRAGEIALLERKRE
                     LQALKEVIK"
     gene            2421..3533
                     /locus_tag="PK768_05310"
     CDS             2421..3533
                     /locus_tag="PK768_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQA60421.1"
                     /translation="MISCQTLVQILEKWAPKKLAMDWDNTGLAIGDLSGEVSRVLLTL
                     TVTPKSIEFAAKNSYEMIISHHPLFFSPLKSLKRDTALGKIIYKAIKNDIVIYSAHTN
                     LDVASGGINDILAERFNLEQVQVLKQTYRESLFKLVVFVPIAYADAVRNAMCDAGAGH
                     IGNYSHCTFNTDGIGTFVPLAGTSPFIGEQNKLERTDEIRIETIVPEGILKRVINAMI
                     KAHPYEEVAYDIYPLENPGKEFGLGRIGYVKEPITLKEFCKTVKHKLNIPYLRVIGDL
                     NKKISKVALCGGAGADLIQAAAFFGADVLVTGDIKYHEALDAEDLGLAVIDAGHFATE
                     NIVLPALKEYLEEQTKLLKKEVKISVYEDVDPFVTI"
     gene            3577..4284
                     /locus_tag="PK768_05315"
     CDS             3577..4284
                     /locus_tag="PK768_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQA60422.1"
                     /translation="MLRDELYNLFKLQELQDRCESLEESLKSHPDLKHLFELKKDANE
                     MRAKLQEKNKRIHDLEKQLRHMEQTVHDSYFAAKQLEERIYSGEVSTVKELKILEEKR
                     NSAIHKAEDYEEKALMAMEELDILKTQLPGEIKLAEQMKTQYREKQLKINEEIGKIKT
                     ELGSIKKQLQQLEASISPELLEKYYRIKKLKTHPVAFITEGKCDGCMMEVSVMLAAEV
                     KQHKRIIYCENCGRILV"
     gene            4307..4729
                     /locus_tag="PK768_05320"
     CDS             4307..4729
                     /locus_tag="PK768_05320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HQA60423.1"
                     /translation="MSKVIVFTDGASRGNPGHAGIGILFLDEKGNVIREISEYIGETT
                     NNIAEYTALVTALREAIDMNFDEIEVMSDSELMVKQINGEYQVKNQGLKPLYSEACTL
                     LREFRSYTVRHVGREYNKRADVLANLGIDEALDEEMVL"
     gene            4804..5175
                     /gene="rnpB"
                     /locus_tag="PK768_05325"
     ncRNA           4804..5175
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK768_05325"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5205..>5630)
                     /locus_tag="PK768_05330"
     CDS             complement(5205..>5630)
                     /locus_tag="PK768_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQA60424.1"
                     /translation="QTFHKVGDKNYVHCTASGKAQIAFSPEAECDDIFQRWGLPKQTK
                     NTITDINVLKEQLREVRVRGYAIDDRESEDDVMCVASPIFDYTGNVVAALSISGPITR
                     LTVEKLEKDFSHLIVDVTADVSKELGYSDKLNFQKTDIS"
BASE COUNT         1964 a         1021 c         1294 g         1351 t
ORIGIN      
        1 acagaaagaa agtggcaaac aatggattgc attataaaaa aagccaaaca agttataata
       61 ataaggaatt taagaaaata tcaccgggaa caggatttta taaagccgag aggaagttgc
      121 tcaaattaat catcgaaaat gaatcaatac gtcgaaatgt aggggaaaag atcgatgaga
      181 gatattttac gaatgacaga acaaagaaaa tagcagatgt tctgtttaaa acaatccggc
      241 aaaatacaaa tcaggatata gatgtttcta atttgtttaa ttacctggat gaagaaagtt
      301 ctgctgaact ttcgtcaata ctagctgaac gtgcagaatt gataaatgaa gaacttattt
      361 gcagtcttgt gacgaaggta aaagaaggct attttaaaga atctattgtc aaagtgaggg
      421 aacaactgaa gcaagctgaa atactaggca aaagagagga gatcaaccag ctgttgaaca
      481 tttatcaaca actcaaggct gaaatggatg agttgaattc tcatactact ccggggaagg
      541 agggagcata aatggcaaac aaagagcaac cgcaaaaaaa ggctgataaa aaacctttac
      601 aggataaaat gaaagcagca accgatctga tagagaaagg caagaaaact ggcaaactta
      661 cttacgaaga gattatggat gctttagacg aaattgaaga tctcgatgct gatcagattg
      721 ataaaatata tgaatctcta gaggaaatgg gtatagaagt attagatggc agcgaaatag
      781 ttgttcctgg agaagaagga ctagatgatg tttctgagga agaccttgac ctctcggttc
      841 ctgagggagt tgctatcgat gaccccgtaa ggatgtattt aaaggaaata ggtaaggttc
      901 cgcttctttc agcggaggaa gaagtagaac tttcaaagcg aatagagaaa ggtgataaag
      961 aagccaagcg aaaacttgct gaagcaaacc tgcgtttggt agtcagtatc gccaaaaaat
     1021 atgtaggcag aggaatgctc ttcctagacc tcattcaaga aggcaatctt ggcctcatga
     1081 aggctgttga gaaatttgat taccgcaagg gttataagtt tagcacttat gcaacctggt
     1141 ggattagaca ggcgatcact cgggccattg ccgatcaggc aaggacaata aggattcctg
     1201 tgcacatggt agagaccata aacaaactga ttcgcgtgca aagacaatta ttgcaggagc
     1261 tcggtcgcga ccctctaccg gaagagattg ccgaagagat gaacctgact gtggaaaaag
     1321 ttagggaaat aatgaagata gctcaggagc cggtttccct ggcgacacca attggtgaag
     1381 aggaagatag tttcctggga gactttatcc ccgacgatga agcattggcc cctgccgatg
     1441 cagcagctta tctcctgtta aaggaacaac tagaagatgt gttagaaaca cttacaccaa
     1501 gagaggaaaa ggttttaagg ctcaggttcg gcttagatga tgggcgcccc aggacccttg
     1561 aggaagtggg acaggtcttt ggagttacgc gggaaaggat aagacagatt gaagcaaagg
     1621 cattaagaaa gcttcgtcat cccagccgca gcaaaaaact taaagatttt cttgaataaa
     1681 agtataatag tggggattta tctccactat tttacttgga cttgagaggt tacggcaaac
     1741 atgaaacttg caaacaggtt gaaaataata gcatcaatga tcgaaaaagg atcgacggtg
     1801 gtagacatag gaagcgatca tgcatatctt cccatatact tagtgaagga aggtatttct
     1861 caaagagtta tagctactga aatactttcc gggccatttg aaagggcccg tgaaaacata
     1921 aaaaaatcct gctaccaaga ctttatccaa ctaagatacg gttctggcct taaaccgata
     1981 aagcccgggg aagtagatgt cgcagtaatt gcaggcatgg gagcaaatac catgataaat
     2041 atcattgaag aatcccggga gactgtagat tctttaaaaa agcttatact tcaacctatg
     2101 tgcaatcagg caaggttaag aaaacatctg tttcaaaggg cttatacaat tatagatgaa
     2161 gatgtagcaa tggatgccaa taaatactac gagattattg tggcacaaaa agcgagcatt
     2221 gtggcttttg atgaaattga catattagtg ggaccggtgc tgaggtgcaa aaagacacct
     2281 gtcgtagagg aatacattca ctacagaata aaaaaactac aaaagatact tgaaaccctt
     2341 gaagcttcta attccagggc aggcgaaata gcccttttag aacgaaaaag ggaattgcag
     2401 gcgttaaagg aggtcataaa gtgatttctt gccagacctt agttcaaata ttggaaaagt
     2461 gggcgcccaa aaagttggca atggattggg acaatacagg tcttgccatt ggtgaccttt
     2521 ctggagaagt atcgagagtc ctactgacac tgacagttac tccaaaatcc attgaatttg
     2581 cagccaaaaa cagttatgaa atgataatat cccaccaccc tctgttcttc agtccattga
     2641 aatcattaaa aagagataca gctctgggaa agataattta taaagcgatt aagaatgaca
     2701 tagtgatcta ctctgctcat acaaacctgg atgtggcctc aggcggaatt aacgacatat
     2761 tggcagaacg gtttaattta gagcaggtcc aggtattgaa acaaacatac agggagagct
     2821 tatttaaact ggtagtgttt gtgccgatag cttatgcaga tgctgtcaga aatgccatgt
     2881 gcgatgccgg agcaggtcat ataggaaatt acagccactg cacatttaac accgatggta
     2941 tcggcacctt tgtccccctt gcaggcacaa gcccatttat cggcgagcaa aataagctgg
     3001 agaggacaga tgaaataaga attgagacaa tagtccctga aggcattcta aaaagggtca
     3061 tcaatgccat gatcaaagct catccctacg aagaagtagc atatgacatt tacccccttg
     3121 aaaatccagg aaaggaattc ggccttggcc gcataggcta tgttaaagaa ccaattacct
     3181 tgaaagaatt ttgtaaaaca gtaaagcaca agctaaatat cccatacctc agagtgatag
     3241 gggatttaaa taaaaaaata tcaaaagtcg ccctttgcgg cggtgccggc gcagatttaa
     3301 tccaggcggc agcctttttc ggtgcagatg tgcttgttac cggtgatatt aaataccacg
     3361 aagcattaga tgctgaagac ttaggccttg cggtaattga tgcagggcat tttgctaccg
     3421 agaatattgt acttcctgcg ttaaaagaat acttggaaga acagaccaaa ttgttgaaaa
     3481 aagaagttaa aatatcggtg tacgaagatg tagacccttt tgtaacaata tgagaatagg
     3541 agtatcctat tctctttatt ttttaggagg gaaacaatgc taagagatga gctgtacaat
     3601 cttttcaaac ttcaggaact ccaagacagg tgcgagagtt tagaggaaag tttaaagagc
     3661 catcctgatt taaagcactt atttgaacta aaaaaagacg cgaatgaaat gcgagcaaag
     3721 ctgcaagaaa aaaacaaacg gattcatgat ttggaaaaac aattacggca tatggagcaa
     3781 actgtccatg attcttactt tgcagcaaaa caactggaag aaagaatcta cagcggagag
     3841 gtaagcactg ttaaagaatt aaagatactc gaagaaaaac gcaactcagc catacataaa
     3901 gcagaggatt atgaggaaaa agcattgatg gcaatggaag aactcgatat actgaagact
     3961 cagcttcccg gggaaatcaa attagctgaa cagatgaaga cacaatacag agaaaaacaa
     4021 ttgaaaatca acgaggaaat tggtaaaatt aaaacggaat taggatcaat aaaaaaacaa
     4081 ttacaacaac ttgaagcaag tatatctccc gagcttttgg aaaaatacta caggattaaa
     4141 aaattaaaaa ctcacccggt agcatttatc acggagggaa aatgcgatgg ctgcatgatg
     4201 gaagtgtctg tcatgttagc tgcagaggtc aagcagcata aaaggataat ttattgtgaa
     4261 aactgcggga gaatacttgt gtagacaaca aaaaaaggga ggccttatga gtaaagttat
     4321 agttttcaca gacggtgcat cccggggcaa tcccggacat gccggcatcg gaatcttgtt
     4381 tttagatgaa aaaggaaatg tcataagaga aattagcgag tacattggtg aaactacaaa
     4441 caacatagct gaatacacag cgcttgttac cgctttaaga gaggcaattg atatgaattt
     4501 tgacgaaata gaagtgatgt cagacagcga actcatggtc aaacaaatta atggagaata
     4561 ccaggtaaaa aatcaaggcc taaagccgct ctactcggaa gcatgtacat tacttaggga
     4621 atttagaagt tatacagtca gacatgtcgg tagggagtac aacaaaagag ctgacgtttt
     4681 ggccaacctg ggaatagatg aggccctcga cgaggaaatg gtcttgtaga aactaccagg
     4741 taccaatgac ttgacaataa aggcctgtat gttataataa cagtccctta aaaacattaa
     4801 tattgagtaa atcggatggt cgcgggcatt ttgcctgagg aaagtccgag ctccacaggg
     4861 cagggtgctg ggtaataccc agtgagggcg acctcaagga aagtgcaaca gaaatatacc
     4921 gcctaacagg tgtcaggagc atgatggtaa gacctctgca cctgttacgg caagggtgga
     4981 aaggtgaggt aagagctcac cagcgggatg gcgacttccc ggctctgcaa accccacttg
     5041 gagcaagacc aaatagggga gcttatacgg cggcccgtcg tgctcccggg tatggtcgct
     5101 ggaggtgtca ggcaactggc atcctagata gatgaccatc taagacagaa ctcggcttac
     5161 agatttactc aataaactaa aaactcgcat aacaatgcga gtttttagct aatatcagtt
     5221 ttctgaaaat tcaatttatc actgtatcct aattcttttg acacatcagc cgtaacatcc
     5281 acaataagat gcgaaaagtc cttctctaat ttttccaccg ttaatctggt aataggacct
     5341 gaaatgctta aagcggctac tacattgccg gtatagtcaa aaattggaga ggcaacacac
     5401 atcacatcgt cttccgactc tctgtcatcg atagcatagc cgcgaaccct aacctctctc
     5461 aattgctctt ttaagacatt tatatcggtg atcgtgtttt tcgtttgttt gggcaagccc
     5521 catcgttgaa aaatatcatc acattccgcc tcgggggaaa atgctatttg ggccttaccc
     5581 gaagcagtgc agtgaacata gtttttatct cctaccttat gaaaagtctg
//
LOCUS       DAONUO010000114         5596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10668_length_5596_cov_3.413644, whole genome shotgun sequence.
ACCESSION   DAONUO010000114 DAONUO010000000
VERSION     DAONUO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10668_length_5596_cov_3.413644"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PK768_05335"
     CDS             complement(<1..1269)
                     /locus_tag="PK768_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA60425.1"
                     /translation="MLTTKLAKILLLVTVFALVLCGCSGNKDESKTPANDDLSLTELT
                     EAAQSEPTYGGSITVGITQDLDGLDPHKAISAGTKEVLFNIFEGLVKFDPDGNLNPAV
                     AENYHISSDGMVYTFTLREGVKFHDGSLVSVEDVIYSIKRSAGILEPKDPTVVVESAL
                     SVISDVVATDEKMVEVRLKQVDSELLPYLTCAIVPKDYDELNTKPIGTGPFKFVSYTP
                     LQSIVLEKNEEYYLEGVPYLDRVTFKISSNVDAAFMELQAGAIDILPYITDVHASQLP
                     KGYHLEAGPMNLIQGLFINNKVPPFDNKLVRQALCYAVDRQAVIDMVAGGRGRVIGTN
                     MYPGFKKYYDESLVNTYPYDPEKAKELLKEAGHPNGFQFTITVPSNYQPHIDTAQVIV
                     EQLKQVGITAKIQLIEWSSWKSVVYKGRNYE"
     gene            complement(1328..2092)
                     /locus_tag="PK768_05340"
     CDS             complement(1328..2092)
                     /locus_tag="PK768_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60426.1"
                     /translation="MIRLKDVTAGYKGVEVIKNINIVFKKGSITSIIGKNGSGKTTLL
                     KTASKLLKPYSGDIFLNDESIYNIPGKQMAKKISFLPQIRNIPNISVYNLVIHGRFPY
                     LGFPRIPRDEDKAKVEHALEKLEVKRFRDKNVAELSGGQRQKVYIAMILAQDTEYIFL
                     DEPTTFLDINHHLEILEIIKELKNMGKAVIMVLHDLNSALSYSDKICIMDRGELVAYG
                     SPQAVYSSGEIERIFNVKCDKVYIEGKSQSQYVFSI"
     gene            complement(2085..3137)
                     /locus_tag="PK768_05345"
     CDS             complement(2085..3137)
                     /locus_tag="PK768_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQA60427.1"
                     /translation="MKAIDTLQRSYTRKFLIKNTASYNIFVIASLAFILLFFIALSIS
                     VGSSSIPLAKTFSVLFGEDVVSKEFQIISYVRIPRTLAAVLAGCALSMSGLILQTVLN
                     NSLAGPSIIGVNSGAGLFTTLIIALFPAYFAFMPIAAFIGALLATLLVYFIAKTTGAS
                     RLTIVLAGAAVSSFIGAMTDTILTVWPDTAISRTSFVIGGLSGVTMDALRLPGILIFV
                     SFMAAMIFSYDMNVLSLGDETARSLGLNVARYRFLFIVLSSLFAGSAISFAGLLGFIG
                     LIVPHASRFIVGYDNRMLVPVISLLGSVFALVCDILARTLFAPYEVPVGIIMSFLGGP
                     FFIFLLLNHRRRLISD"
     gene            complement(3085..4050)
                     /locus_tag="PK768_05350"
     CDS             complement(3085..4050)
                     /locus_tag="PK768_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA60428.1"
                     /translation="MRRKILIILVLLMVIVAGCSNDKVDETAHVDSEGSKAYYSFTDS
                     LGNSVMLQEKPQRVVSLVGSYAQTWMLAGGEVIGVTDDVISEGRFEISEEISIVGTVK
                     EPNVEEVLSLSPDFVLLSPDIENHLKIRQILEDAAIPHAYFKVEYFEDYLKMLDICTD
                     ITGNKDLYEEHGLKIKEEIDGILSRIEDAGLDHKPTVLFVRAFSSGAKAKKDDNLTCK
                     MLEDLGTVNIASKYPSLLEELSIEEIIQEDPDYIFVTTMGSTEKAIEALRNGIEKNPA
                     WNSLTAVKNDRYIILPKELFHYKPNAKWGESYRYLAEIIYPEIFN"
     gene            complement(4185..5108)
                     /locus_tag="PK768_05355"
     CDS             complement(4185..5108)
                     /locus_tag="PK768_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQA60429.1"
                     /translation="MTIRHLRIFIEVVKEGSMRAAAAKLYISQPTVSQAIRELEEHYG
                     VLLFERLKRRLYITDAGKKLFAYAKRVLKEFDELEEKMLGLGRREKIRIGSTITVGES
                     ILSDLINELTKKEPNIEVYSYMNNTRAIEAKLLNYEIDIGIVEGEIKSPDLICIPEVK
                     DYLVLICSTDHPFAKRKTVKLKELANESFAMREKGSGTRELFERYMIDSGIEIKIAFE
                     GNSPEAIKKEVINNSYLAVLSVLLVETEVKNSQIHVIECADGAWDRYFSIVYHKDKVL
                     TKGMESLIGIIKDYKDASSRIITDSPSKLVK"
     gene            5257..5544
                     /locus_tag="PK768_05360"
     CDS             5257..5544
                     /locus_tag="PK768_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA60430.1"
                     /translation="MKVLILGGVAAGTKVAAKLKRDNYDTEVTILTKSKDISYAGCGL
                     PYYVGNVIENKEDLIVNTPESFSKPIGVEVLTEVEVTKVHPDKKEVEALDL"
BASE COUNT         1566 a         1300 c         1024 g         1706 t
ORIGIN      
        1 ctcatagttg cgacctttat atacaacgga cttccagcta gaccattcaa tcagctgaat
       61 cttggctgta atgccaacct gttttagctg ttctacaatg acctgggcgg tatcaatatg
      121 aggctggtaa tttgaaggaa ctgtaatcgt aaactgaaaa ccatttggat gacctgcttc
      181 ttttaacagt tccttagcct tttcgggatc atagggataa gtattcacaa ggctttcatc
      241 ataatacttc ttaaatccag ggtacatatt ggtaccaatt actctgcccc gccctccggc
      301 gaccatatca attacagcct gccggtctac tgcatagcaa agagcttgtc taaccagttt
      361 attgtcaaaa ggtggcactt tattatttat aaacaggccc tggatcaggt tcattggtcc
      421 cgcttccaag tgataacctt tcggaagctg agaagcatgg acgtcggtaa tatatggaag
      481 aatatctatg gcccctgcct gtagttccat aaatgcggca tccacgtttg atgatatctt
      541 gaatgtaacc cggtcaagat aaggcacacc ttccaaatag tactcctcat tcttctccaa
      601 gacaatgctc tggagcggcg tataagatac aaacttaaag ggtcccgtac ctataggctt
      661 ggtattcaac tcatcataat cctttggcac gatagcacat gttaaatacg gaagcagttc
      721 gctgtccacc tgtttcaggc gaacttcaac catcttttca tcggtagcaa ccacgtcgga
      781 aatcacagaa agcgccgatt ctactacgac cgttggatct ttcggctcaa gtataccggc
      841 gcttcttttt atggagtata taacatcctc tactgaaaca aggctgccat catggaattt
      901 tacgccttcc ctcagggtaa aggtataaac cattccatca ctggaaatat gatagttttc
      961 tgccacagcc gggtttaaat ttccatcagg atcaaacttt actaagccct caaaaatgtt
     1021 aaatagaact tctttggttc ctgctgatat agctttatga gggtcaagac catccaaatc
     1081 ctgggttata cctacagtga ttgaacctcc ataagtcggc tcagattgtg ccgcttctgt
     1141 aagttcagtc aaggacaaat catcattcgc aggagtttta ctctcatctt tattgccact
     1201 acatccgcag agcacaagag caaacacagt cacaagaagc aatattttag ccagttttgt
     1261 tgtaagcatg aaaacacacc ccactaatga tttagtctca ttatttaagg atagctttgt
     1321 tttcatttta tatagaaaaa acatactgac tttggctctt tccttcgata taaaccttgt
     1381 cacacttcac gttaaatatc cgctcaattt ccccgctgct atatacggcc tgagggctgc
     1441 catatgctac tagttctccc ctatccataa tacaaatttt atcactatag ctcaatgcgc
     1501 tgtttaggtc gtggagcacc atgattacag cctttcccat gtttttaagc tcttttatga
     1561 tttccaaaat ctcaagatga tgatttatat ctaaaaaagt tgtcggctca tctaaaaata
     1621 tatactctgt atcttgggct aaaatcatcg ctatatacac cttttgccgc tggccccctg
     1681 acagctctgc tacatttttg tccctgaatc ttttgacttc cagcttttcc aaggcatgtt
     1741 ctaccttggc cttgtcttca tcccggggta ttcgtgggaa tcccaaatag ggaaatctgc
     1801 cgtgtatgac cagattatag acggatatat tgggaatatt tcttatttga gggagaaagg
     1861 atatcttttt tgccatctgc ttgccgggta tgttatatat actttcatca tttaggaaaa
     1921 tgtctccgga gtagggcttc aacaacttgg aggctgtttt tagcaaggtg gtctttccac
     1981 tgccattttt tccaattata ctggttatgc ttcccttctt aaatacaata tttatatttt
     2041 ttatcacctc aacgcctttg taaccggcgg ttacatcctt taacctaatc acttataagc
     2101 ctcctcctat ggttgagaag cagaaagata aagaaaggtc ctcccaaaaa tgacataatt
     2161 attccaacag gaacttcata tggggcaaat aaggttctag ccaatatgtc acaaaccaaa
     2221 gcaaatacgc tacccaggag ggagataacc gggaccaaca tcctgttgtc atatcccact
     2281 ataaatctcg aagcatgggg cactataaga cctatgaaac ccagcagacc tgcaaagctt
     2341 atggcactac ctgcaaacaa tgatgacagc acgataaaca aaaagcggta tctggccacg
     2401 tttagcccca aggacctggc cgtctcatcc cccagggata aaacgttcat gtcatagcta
     2461 aaaatcatgg cagccataaa agatacaaaa attaggattc cgggcagtct tagagcatcc
     2521 atggtcacac cggaaagacc tccaattacg aagcttgtcc tgctaatggc cgtatctggc
     2581 catacagtga gaatcgtgtc agtcatggcg cctataaagc tggacaccgc tgcaccggcc
     2641 aacactatgg taagccttga agccccggtt gtctttgcta taaaatatac cagcaatgtg
     2701 gccaaaaggg cgccaataaa agccgctata ggcataaaag caaaatatgc gggaaagagg
     2761 gcgataatca aagtagtaaa aagtcctgcc cccgagttta cccctattat gcttggccct
     2821 gccagggaat tgttcagcac cgtttgcaat atcaagccgg acatggacag ggcacagcct
     2881 gccaagactg ctgccagggt cctgggtatc ctgacgtagc ttataatttg aaattccttt
     2941 gaaactacat cttctccgaa taagacagaa aaggtcttgg ccaagggtat gcttgacgag
     3001 ccaacactta tgctaagtgc tatgaaaaat agcaatataa aagcaaggga agctataaca
     3061 aagatgttat agcttgcggt attcttaatt aaaaatttcc gggtatatga tctctgcaag
     3121 gtatctatag ctttcacccc actttgcgtt cggcttgtag tggaacagct ccttgggaag
     3181 gattatatat ctatcgttct ttacggcagt taagctgttc caagccgggt ttttttctat
     3241 tccgtttctt agtgcttcaa tggccttttc agtgctgccc attgttgtga caaatatata
     3301 atcaggatct tcctggatta tctcctcaat gctcagctct tccaaaagag atggatactt
     3361 gctggcaatg tttacggttc ccaagtcttc cagcatcttg caagtcaaat tgtcgtcctt
     3421 tttagctttg gccccactgg aaaaggcgcg gacgaagagc acggtaggct tgtggtctaa
     3481 tccggcatcc tctattcttg ataatatacc gtcgatttct tcctttatct taagaccatg
     3541 ttcctcgtat aaatccttgt ttcccgtaat gtcggtgcaa atatccagca tctttaaata
     3601 atcctcaaag tactccactt taaaataggc atggggaata gctgcatctt ccaatatctg
     3661 tctaatcttc aagtggtttt ctatgtccgg tgatagcaaa acaaaatcag gggacagaga
     3721 tagtacttcc tcaacattgg gctcctttac tgttcccaca atgctgattt cctcagatat
     3781 ctcaaagcgt ccttcactta ttacatcatc ggtgacccct attacttccc cgccagccag
     3841 catccaggtt tgggcatatg agcccaccag ggatacaact ctttggggtt tctcctgaag
     3901 cattactgaa ttgccaaggg aatcggtaaa gctataatat gctttactgc cctcgctgtc
     3961 tacatgggca gtttcatcga ccttatcatt gctgcatcct gctactataa ccatcagcaa
     4021 aactaatatt atgagtatct ttcttctcaa ttccagcact cctacattga tattttcatc
     4081 acaatcaatt tgttaatttt tccacatatt aatagtagca ttttataaat tgttttaaaa
     4141 ctacaataat ccaatcataa taatagcttt tacctatatg tcaatcattt taccaactta
     4201 ctcggcgagt cggttattat acggcttgat gcatctttat agtcttttat aatcccaatt
     4261 aagctttcca ttcccttagt caataccttg tccttgtggt aaacgatact gaaatatctg
     4321 tcccaagctc catctgcaca ttcaataacg tggatttgtg aattcttaac ctcggtctcc
     4381 actaacaaaa cagagagaac cgctaaatag ctgttgttta tcacctcttt ttttatggct
     4441 tccggactgt tgccttcaaa ggctatcttt atctctattc cgctatcaat catatatctc
     4501 tcaaagagct ccctcgtccc actgcccttt tcacgcatgg caaaactttc attggccagc
     4561 tcttttagtt ttacggtctt tctttttgca aagggatggt ctgtgctgca gatgaggact
     4621 aaatagtcct ttacttccgg aatgcatatc aaatcgggac tctttatctc cccttctact
     4681 attccaatat ctatttcgta gtttaaaagc ttagcctcaa tggcccgggt gttattcatg
     4741 taggagtaaa cttcgatgtt gggctctttt ttggtcaatt catttatcag atcgctgagt
     4801 atactctcac ctaccgttat ggtcgaccca atccttattt tctcccttct tccaagacca
     4861 agcattttct cttccagttc atcaaattct ttcaaaactc ttttagcata tgcaaatagt
     4921 tttttccccg catcagttat ataaagtctc ctctttaacc gctcaaagag caaaaccccg
     4981 tagtgctctt ccagttccct gatggcctgg ctcaccgtag gttgcgatat atagagttta
     5041 gccgctgcag ccctcatact gccctctttt acaacctcga taaaaatcct caaatgcctt
     5101 atcgtcacct tatacctcca taagtgaaaa atctttgttt tgacttttat aggttttacc
     5161 tattactgtg atttaatttt atcattttac cattttttaa acaattgtta tagttaaatt
     5221 agagattgtg aatttgtttg caaggagcgt tgcattatga aagtattgat attaggcggt
     5281 gtggcagcag gcaccaaagt agctgcaaaa ctaaagaggg acaactacga cacagaagta
     5341 accatactta caaaaagtaa ggatatttcc tatgcaggat gcggcctgcc ctattatgtc
     5401 ggaaatgtca tagagaataa agaagacttg atagtcaata ctccggaaag cttttccaag
     5461 ccgatcggtg ttgaagtgct gactgaagtc gaagttacaa aagtacatcc cgacaaaaaa
     5521 gaagtcgaag ccctcgattt ataaacaaat gaatgcggaa catacaggta cgacaaactt
     5581 gtcatcgcta ccggtg
//
LOCUS       DAONUO010000115         5569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10727_length_5569_cov_4.450852, whole genome shotgun sequence.
ACCESSION   DAONUO010000115 DAONUO010000000
VERSION     DAONUO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10727_length_5569_cov_4.450852"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..381
                     /locus_tag="PK768_05365"
     CDS             247..381
                     /locus_tag="PK768_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HQA60431.1"
                     /translation="MEYMGPVRLRDVEDAQQKIVNIIRKLEDAGEIIISRGGGDEIIV
                     "
     gene            374..1111
                     /locus_tag="PK768_05370"
     CDS             374..1111
                     /locus_tag="PK768_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HQA60432.1"
                     /translation="MSKVLKFAVLEKGNPIKLKEPQNPATTKDSEKALINDLSARKFF
                     EEEGNRLLNEAAAKAREIVEKSKEEAQKIIAMAQTDKENIEKEAFDKGYSDGFEAGKK
                     EQELLWNKYLIELDKTKQELKKQNAVFREHLEKECIRLSLAIAEKILGKAIETDIEYF
                     IDLIKKGLEEAGEDKEALIRISEEDYDRVSTLISKLKGQHSITLLKDPTLSSGDCVIV
                     GPNYEINSGIRTQIENVAEALRKLEVI"
     gene            1111..2436
                     /gene="fliI"
                     /locus_tag="PK768_05375"
     CDS             1111..2436
                     /gene="fliI"
                     /locus_tag="PK768_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HQA60433.1"
                     /translation="MYSKNFDYVYSILDDIVTIKPKGKISKIVGLTIESEGPPAEMGE
                     ICIVKSPNNPKTILTEVVGFRDETVILMPLGDMEDLGPGWEVEASRNKMTVAVGEELL
                     GRVLDGLGNPIDGKGPIKTNYSYPVLNNPPNPIERPIIKEALPLGIRAIDAFITCGVG
                     QRLGIFAGSGVGKSTLLGMIARNTKADVNVISLVGERGRELNSFIKKDLGEEGLKRSV
                     IVVATSDKPPLIRTRAAFTATAIAEFFRDQGKNVLLMMDSITRFAMAQREVGLAAGEP
                     PVTRGYTPSVFATLPKLLERAGTSSKGSITGIYTVLVDGDDFNEPISDAVRGILDGHI
                     VLSRNLANRNHYPAIDVLSSISRLMNDIVSPKHSQLARRVKSILSVYKEAEDLINIGA
                     YSKGNNPKIDEALKYIDQIEGFLKQGIEEKIEFDKTIEALEMIFEDVDL"
     gene            2433..2900
                     /gene="fliJ"
                     /locus_tag="PK768_05380"
     CDS             2433..2900
                     /gene="fliJ"
                     /locus_tag="PK768_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HQA60434.1"
                     /translation="MKKFYFRLETPLRIKQLKEKMEKQKLSQALYQKRSEENKLAEME
                     LTKDNIREELDNKLIDSVEIRDLSDFSVFASKMIKLIEAQKKIVEHVSELCEESKRDF
                     MKSRIERQIYEKIKEYHYMEYNKMVYREEQKISDELANINHGRLERNLANEQY"
     gene            2887..3426
                     /locus_tag="PK768_05385"
     CDS             2887..3426
                     /locus_tag="PK768_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE"
                     /protein_id="HQA60435.1"
                     /translation="MSSINGKSILIGIVIFLVLVSVAFYFLYFRNLDSAHPLKTALAS
                     LPFFKKESSQQLIEEEDLEEEKARIEHEWAKLDSEKKQLADKEAQLAKKESELEDLER
                     ELNAERERLETVITNIKDLARYYELMEAKDAAAILENMEDELLIQLLRNMKKETTSEI
                     LSNLDAKRAAEITKKMSGL"
     gene            3466..4707
                     /locus_tag="PK768_05390"
     CDS             3466..4707
                     /locus_tag="PK768_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HQA60436.1"
                     /translation="MALQVFQLDANMTKTVDNRKTFTTSKGNEFNKLLGKIKELTGKR
                     KENEVHPLLMQPMNILNIDTSWLGFEELLEQQHTSSFTNLENILYDKLPTDELNRCWQ
                     AILAEFNITGDVKLDTIEKFHSALLKALPHDADLEPNILRDAISDILRASNLKPKTNS
                     FNSKLSNNNLVEHSMNEVTNLEDKNVKGEELEADLLRDESELSIRDELNHDKRVFVED
                     NFGSYEKSDEHTQQEIGPDSQQQDIFLASHTQKSTAASTGKAITISTSSSESPDVFDQ
                     LIDKVRFALKGEVQEIKVRLKPEYLGDVMIKVISEKGKLKAELFVGNTHVRSMLKAHA
                     IEFQNQIREQGYNFSEINVYKMDEGFEMGTFDHRSGGNNSYQGKKSKGNSYFEVTESQ
                     QSTVTDNYGLWGYINNINYMA"
     gene            4719..5102
                     /locus_tag="PK768_05395"
     CDS             4719..5102
                     /locus_tag="PK768_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HQA60437.1"
                     /translation="MNVINTEYNVSNQQQKLGTTNLGKDDFLKLLVTQLRYQNPLEPM
                     DNKEYIAQLAQFSSLEQMQNLNMQLASLSAINTIGKTAKAVVEQKEIEGIIKGIAFDK
                     GRVNLIIVGNESEIKVPIENVAEIR"
     gene            5116..5502
                     /locus_tag="PK768_05400"
     CDS             5116..5502
                     /locus_tag="PK768_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HQA60438.1"
                     /translation="MQDYTRIKTGINISVSPVTQGVNKPQQIHQKGFEKILQQKIEEK
                     KELKISQHAQRRMDMRNIRLTQEQKQLLNSAVEKASQKGVKESLILMNDLAFVVSIKN
                     RTVITAMDGDSIKENVFTNIDGAVII"
BASE COUNT         2045 a          910 c         1215 g         1399 t
ORIGIN      
        1 tcattggtga tggtctctag cccggtttct agttcttcta gtttttttat cttaggctcg
       61 ctcataaaac tcaggatttt attgatgaaa tttatgcagt tttgtattac aatagtattc
      121 ataagagatc ctccttttca atggttcttt atggtgattc cattgtatca aaaagtgagg
      181 atctcttgtt cttttttatc ctgaaaatgt cctacaatta ttttacacta tgtgataaga
      241 gaggacatgg agtatatggg ccctgtacgt ctaagagatg tagaagatgc ccagcaaaaa
      301 attgtcaata ttatacggaa attagaggat gccggtgaaa taatcatttc ccgtggcggt
      361 ggtgatgaaa taattgtcta aagttttaaa gtttgcggtt ctcgaaaagg gaaaccccat
      421 aaagttgaaa gaaccccaaa atccagcaac tacaaaagat agcgaaaaag cccttatcaa
      481 tgatctttct gctcgaaagt tctttgaaga agaaggcaat cggttactta atgaagcagc
      541 tgctaaggca agagaaatag tcgaaaaatc aaaagaagag gcacaaaaga tcatagcgat
      601 ggcccaaact gacaaagaaa acattgaaaa agaggctttc gacaaaggat actctgatgg
      661 ttttgaagct gggaaaaaag aacaagagct tctttggaat aaatatttga tagaacttga
      721 caaaaccaaa caagaactaa aaaagcaaaa tgccgttttt agagagcatt tggaaaaaga
      781 atgtattaga ttatcattgg ctatcgccga gaagatatta ggcaaagcca tagagaccga
      841 tattgagtat tttatagact tgataaaaaa aggccttgaa gaagccgggg aagataaaga
      901 agcattgatt agaatctctg aagaagatta tgatagggta agcacattaa tctcaaaact
      961 caagggccaa cattccatca ctttgctaaa agaccccaca ttgtcttcag gtgactgcgt
     1021 aatagttgga cctaattacg aaattaattc gggtatccgc actcagatag aaaatgtcgc
     1081 ggaagcctta aggaaactgg aagtaatatg atgtatagta agaattttga ttatgtttat
     1141 tccattttag atgatatagt tacaattaag ccaaagggga agatttctaa aattgttggc
     1201 ttaaccattg agtcagaagg accgccagca gaaatgggag aaatctgtat agttaaatca
     1261 cctaataatc caaagaccat attgacagaa gtcgtcggtt ttcgggatga aacagtgatt
     1321 ttaatgcctt taggcgatat ggaagactta ggaccaggat gggaagtgga agctagcaga
     1381 aataaaatga ccgttgccgt gggggaagaa ttactaggca gagtgttaga tgggctggga
     1441 aaccccatcg atggcaaagg acctataaaa acaaattata gctatcccgt cttaaataac
     1501 cctcctaatc caatagaaag acccataatt aaggaagctc tgcctcttgg aattagagca
     1561 atagacgcat tcattacctg cggcgtgggg caaagattgg gtatatttgc tggtagtggt
     1621 gtagggaaaa gcacgctttt aggtatgata gcgcgaaaca ctaaggctga cgttaatgta
     1681 attagtttgg taggcgaaag aggcagggag ctgaatagtt tcataaaaaa ggatttaggt
     1741 gaggaaggtt taaaaaggtc tgttatagtt gttgcgactt cggacaaacc tcccctcatt
     1801 aggactagag ctgcctttac tgccacagct atagcagaat tttttagaga ccaaggaaaa
     1861 aatgtgctat tgatgatgga ctcaattacg aggtttgcca tggcacaacg ggaagtaggt
     1921 ttggcagctg gagaaccgcc tgttactaga ggctatactc cttcagtctt tgccacccta
     1981 ccaaagctcc tggaaagggc tgggacttcc tcgaaaggtt caattacagg catatataca
     2041 gtattagttg acggtgatga ttttaacgag cctatatcag atgccgtccg cggtattttg
     2101 gatgggcata ttgttttgtc gaggaattta gctaatagga atcactatcc tgccatagat
     2161 gttctatcaa gcataagtag gcttatgaat gatatagtca gccctaaaca cagtcagtta
     2221 gcaagaagag ttaagagcat cctctcagta tataaagagg ccgaggattt aataaatatc
     2281 ggagcctact ctaaagggaa taatccaaag attgacgaag ccttaaagta catcgaccag
     2341 atagaaggat tcctcaaaca gggtattgag gaaaaaatcg aatttgataa gaccatagaa
     2401 gctttggaaa tgatctttga ggatgtagat ctatgaagaa attttacttt agattggaaa
     2461 cgccccttag gataaaacag ctcaaggaga aaatggagaa acaaaaacta tcacaagctc
     2521 tgtatcaaaa gcgctcggaa gaaaacaagc ttgctgaaat ggaacttaca aaagacaaca
     2581 ttcgtgaaga gcttgacaac aaattaattg attccgttga aatcagagat ctatcagact
     2641 ttagtgtttt tgcgagtaaa atgattaagc tcatagaagc ccagaaaaaa atcgtcgaac
     2701 atgtaagcga attatgtgaa gaaagcaaaa gggattttat gaaaagtaga attgaaaggc
     2761 aaatatatga gaagattaaa gaataccatt atatggagta caacaaaatg gtctacagag
     2821 aagaacaaaa aatcagtgat gaattggcaa acatcaacca tggccgttta gaaaggaatt
     2881 tagctaatga gcagtattaa cggcaaaagc attttaatcg ggatagtaat ttttttagtg
     2941 cttgtttcag tagcatttta ttttttatat tttagaaacc ttgatagtgc acatccttta
     3001 aaaacggcac tggcaagcct gccctttttt aaaaaagaat catcacaaca attaatcgaa
     3061 gaggaagacc ttgaagaaga aaaagcaaga attgaacacg agtgggccaa gttagatagt
     3121 gaaaaaaaac aacttgcaga taaggaagct caactagcaa aaaaggaaag tgaactggag
     3181 gatttagaga gggagctcaa tgctgaaaga gaaagattgg agactgtcat aaccaatatt
     3241 aaagatttgg caagatatta tgaattaatg gaagcgaaag atgcagcagc catccttgag
     3301 aatatggaag atgagctgct aatacagctg cttcgtaata tgaaaaaaga gactacttcg
     3361 gaaatacttt caaaccttga tgctaaaagg gccgcagaaa tcacaaaaaa aatgagcggc
     3421 ttatagagaa atcataacat atgagaggag gtgaaaggag tggatatggc attacaagtg
     3481 ttccagctag atgccaatat gacaaagaca gtagacaata gaaaaacatt tactacatca
     3541 aaaggaaacg aattcaacaa actccttggg aaaatcaaag aactaactgg taagaggaaa
     3601 gaaaacgaag tacatccgct attaatgcag ccaatgaata tcttaaacat cgatacttcg
     3661 tggctgggtt ttgaagagct acttgagcag caacatacta gttcatttac aaacttagaa
     3721 aacatcctgt atgacaaatt gcccacagat gaattaaaca gatgctggca ggcaatactt
     3781 gcggagttta atataacggg agatgtcaag ctggatacta tagaaaaatt tcactcagct
     3841 ttgctaaaag cactgccaca cgatgcggat ttagaaccga atattcttag ggatgccatt
     3901 agtgatatac ttagggcttc gaacttgaaa cccaaaacaa acagctttaa ttcaaaatta
     3961 agcaacaata atttggtaga acacagtatg aatgaagtga caaatctgga agataaaaac
     4021 gtcaaaggcg aggaactaga ggctgaccta ttaagggatg aatctgaact ttctattaga
     4081 gacgaactaa accatgacaa aagggtattt gttgaagata acttcggttc atacgagaaa
     4141 tctgatgagc atactcaaca agaaattggt cccgattcac agcaacaaga catctttttg
     4201 gcatctcaca cacaaaaaag cactgccgca tcaacgggta aagcaataac aatttccaca
     4261 agcagttctg aatctccgga tgtttttgac caattaatag acaaggtccg atttgcctta
     4321 aaaggagaag tgcaggaaat aaaagttcgc ttaaaacctg agtacttagg tgatgtcatg
     4381 attaaagtta tttctgagaa aggcaagttg aaggccgaac tctttgtagg taacacgcat
     4441 gtaaggtcca tgttaaaagc acatgctatt gaatttcaaa atcagatacg ggagcaggga
     4501 tataactttt cggaaatcaa tgtttataaa atggatgagg gctttgaaat ggggaccttt
     4561 gatcatcggt caggcggcaa caatagctat caaggaaaga agtcaaaagg aaattcttat
     4621 tttgaagtga cagaaagcca gcagtcaaca gttacggaca actatggtct atggggatat
     4681 ataaacaata ttaattatat ggcgtaagga ggggaattat gaatgtaatc aacactgaat
     4741 acaacgtttc aaatcagcag caaaaacttg gcacgacaaa cctaggcaaa gatgactttt
     4801 taaaactatt agtcacccaa ttgagatacc aaaacccgct tgagcccatg gacaataaag
     4861 agtatatagc tcaactagca cagtttagtt ccctagagca aatgcaaaac ttaaatatgc
     4921 aactggccag cctgtcagct ataaacacta ttggaaagac tgcaaaggct gtagtagagc
     4981 aaaaggaaat agaaggcatc attaaaggaa ttgcatttga caaagggcgg gttaacttaa
     5041 taattgtcgg taatgagagt gaaataaagg tgcccattga aaacgtggct gaaatcagat
     5101 aaagaggtgg ggcagatgca agattatacc agaataaaga ccgggattaa tattagtgtt
     5161 tcaccggtta ctcaaggggt aaacaagcca caacaaattc atcaaaaggg ctttgagaag
     5221 atacttcagc aaaaaattga agaaaaaaag gagctcaaaa tttcccaaca tgcccagaga
     5281 cgcatggaca tgcgaaatat aagacttaca caagaacaaa agcaattgct aaatagtgct
     5341 gttgaaaaag ccagtcaaaa gggtgtcaaa gaatccctta tccttatgaa tgacttagct
     5401 tttgtcgtaa gtatcaaaaa caggaccgtc attactgcta tggacggtga tagcataaaa
     5461 gagaatgttt ttacaaatat tgatggtgcc gtaattatat aagccggacc gcaaggaggc
     5521 tttaagctcc ggattgaccg aaggagctgg cacccaaaaa gggaggaat
//
LOCUS       DAONUO010000116         5568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10729_length_5568_cov_4.238164, whole genome shotgun sequence.
ACCESSION   DAONUO010000116 DAONUO010000000
VERSION     DAONUO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10729_length_5568_cov_4.238164"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PK768_05405"
     CDS             <1..1006
                     /locus_tag="PK768_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HQA60439.1"
                     /translation="MDMGHMSPTEHVSFTFAIDGVSRTLLAQITRHRIASFSVQSLRY
                     NNPFDEEIANPKDQALIESESISYLEGYALSSKLSGNSNLKNHHKRKQYGIEGLMPQD
                     VENLHKAAYVRGVFDGAGLICDDSEYPLVFPQELKSVFDVAGLDVSLKEDVVLIQNPS
                     DFGMYIYENMDFAESLYDENKFLSLCERDDKFQKQILAVMQEYIDAKYYAMAPESITT
                     KPKALLSYIKGLDACKKCYIDLVNMGIDQEDARYILPHGTQTRIVMTMNVRSLYNFFN
                     LRCCERAQTEIRQLANMMLEEVKKIAPGLFESAGAPCEALGYCPEGVLSCGKYPSYPN
                     "
     gene            complement(1059..1436)
                     /locus_tag="PK768_05410"
     CDS             complement(1059..1436)
                     /locus_tag="PK768_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HQA60440.1"
                     /translation="MDVDSLSALALAFVGDSVFDLFIRTALVGSGKKVRDLHREAVSF
                     VKASAQADILKKLEPELDSKERDIVRNARNAKVNTVPKHADIADYHYSTAFEALLGYH
                     YLSGNTERLHELLHLAYDVNMNI"
     gene            complement(1473..2873)
                     /gene="cysS"
                     /locus_tag="PK768_05415"
     CDS             complement(1473..2873)
                     /gene="cysS"
                     /locus_tag="PK768_05415"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQA60441.1"
                     /translation="MQIYNTLTRKKEEFVPLKGNKVNIYTCGPTVYDFFHVGNARVFI
                     TFDMIRNYLKFRGYDVTFVQNFTDIDDKMIKRANEENITVKELGERFIDEYFKDADAL
                     GIERADVHPKATEHIEDIIELIKTLIDNGYAYEVDGDVYYATRKFKEYGKLSQQNVDE
                     LEAGARIEPGEKKKDPMDFALWKAQKPGEPAWDSPWGKGRPGWHIECSVMAMKYLGET
                     IDIHGGGPDLIFPHHENEIAQSEAATGRPFARYFMHVGYLNIDNQKMSKSLGNFFTVR
                     DILKKYDPEVLRFFMLSSHYRSPINFSEDLMQQAKSSLERLYNALYSMEHLENLAEDR
                     ELVEKDKEYLDKLEKYEREFVEAMDDDFNTADAIASLFNIVREYNMKVDEKSPREIVK
                     ATKETLLNLGSVLGFFRKFEEKTLLDEEIEQKIREREEARKSKNYALADKIRDELKAQ
                     GIILEDTPSGVRWKRQ"
     gene            complement(3279..3953)
                     /gene="cysE"
                     /locus_tag="PK768_05420"
     CDS             complement(3279..3953)
                     /gene="cysE"
                     /locus_tag="PK768_05420"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQA60442.1"
                     /translation="MFSNIRQDIKVIFERDPAAKSVLEVILCYPGFHAILLHRIAHSF
                     YKLNLVLIARIISQINRFLTGIEIHPGAKIGKGFFIDHGMGVVIGETTEIGDNVTLYQ
                     GVTLGGTGKEKGKRHPTLGDNIVVGSGAKILGPVKIGDNAKIGAGAVVLKDVPPNSTV
                     VGIPGKAVVRKKVDFSDADFTKVDLDHHLLPDPVADMMRSLQKTIETLEMRIAFLEKE
                     LKSKTG"
     gene            complement(4254..4739)
                     /locus_tag="PK768_05425"
     CDS             complement(4254..4739)
                     /locus_tag="PK768_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQA60443.1"
                     /translation="MGKIARVMALLAVFCCLGPIQGGQNTLHAKPVYHNFQEAHPSLR
                     VKPYTKNTLDTKVDTRVGTKIFIGKASWYGPNFHGKLTANGEIFDQNALTAAHRELPF
                     GTKVLVTNLENGKSTLVTINDRGPYIQGRHIDLSRAAAKEVGMIEAGVVDVMMQIVAY
                     E"
     regulatory      complement(4742..4875)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(4938..5417)
                     /gene="ispF"
                     /locus_tag="PK768_05430"
     CDS             complement(4938..5417)
                     /gene="ispF"
                     /locus_tag="PK768_05430"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQA60444.1"
                     /translation="MRIGIGFDVHKLVLGRKLVLGGVVIPFEKGLLGHSDADVLVHAI
                     NDALLGACALGDIGKHFPDTDPEYKDIDSIILLKKVKDLLKDRGYRVINVDSVICAQR
                     PKLAPYIDEMRRNIADTLGIAVEQISIKATTTEGLGFEGREEGISAHAVCLIEKFLD"
     gene            complement(5420..>5568)
                     /locus_tag="PK768_05435"
     CDS             complement(5420..>5568)
                     /locus_tag="PK768_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQA60445.1"
                     /translation="TDDTVLVERLGFPVKIIEGAYENIKITTPEDIVLAEAFISVGYG
                     NYRR"
BASE COUNT         1385 a         1370 c         1159 g         1654 t
ORIGIN      
        1 gatggatatg ggacatatgt ctcccacaga acacgtaagc tttacatttg ccatcgacgg
       61 tgtgagccgc acactgctgg cccagattac acgtcatcgc atagcatcat tctcggtgca
      121 gagtttaagg tacaacaatc cctttgatga agagatagca aatccaaagg accaagccct
      181 gattgaatcg gaaagtataa gctatttaga gggatatgcc ttatccagca agctgtcagg
      241 aaattcgaac ctgaaaaatc atcacaagag gaaacaatac ggtattgaag gcttgatgcc
      301 gcaggatgtg gagaatttgc ataaggccgc ttatgttcgg ggagtatttg acggtgcggg
      361 cctgatttgc gatgattcgg agtacccttt ggttttcccc caggagctaa aatccgtatt
      421 tgatgttgct ggcctggacg tgtctcttaa agaagatgtg gtattgatac aaaacccgtc
      481 agatttcggc atgtatattt atgaaaacat ggattttgcc gagtcgctct acgatgagaa
      541 taaattcctg tccctatgcg aaagagatga taaatttcaa aaacaaatcc tggccgttat
      601 gcaggaatac atagacgcca agtattatgc catggccccc gaaagtatta caactaagcc
      661 caaagcatta ctttcatata tcaagggcct tgatgcctgc aaaaagtgct atatagacct
      721 ggtgaatatg ggcattgacc aggaagatgc caggtacata ctgccccacg gcacccaaac
      781 cagaatagtc atgaccatga acgtcaggag cctgtacaat ttctttaacc tgcgctgctg
      841 cgagcgtgcg caaaccgaaa tccgccagct ggccaatatg atgcttgaag aagtaaaaaa
      901 aatagctccc ggccttttcg aatcggcagg agctccctgt gaagcattgg gatactgtcc
      961 cgagggggtc ctgagctgcg gcaagtatcc ttcataccct aactaagaaa ttttatccac
     1021 gtcatgtgaa tgaatatttt gtacccgtcc cgaaatattc aaatattcat attgacgtca
     1081 tatgccaggt gaagcaactc atgcagccgc tccgtattcc cggaaagata gtgatatccc
     1141 agcagtgctt caaaagctgt gctgtagtga taatccgcaa tgtccgcatg tttcggaacg
     1201 gtgttcacct tggcattcct ggcgtttctc actatatccc tttcctttga atctagctcc
     1261 ggttcaagct ttttcaatat atccgcttgg gcagaggcct tcacgaagct gaccgcttcc
     1321 ctgtgaaggt ctcttacttt tttgccgctt cctacaaggg cagtccttat aaaaaggtcg
     1381 aataccgaat ctccgacaaa ggccagggcc aaggcagata aagaatctac gtccacatta
     1441 tttttggctt ctatacatga tccctcccta ttttactgcc gtttccagcg cactccagaa
     1501 ggtgtgtctt cgagaattat accttgggct ttcagttcgt cccggatttt atcggccaaa
     1561 gcgtaattct tactttttct cgcctcttcc ctctctctta ttttctgctc gatctcttca
     1621 tctaataagg tcttttcttc aaacttgcgg aaaaagccca aaacgcttcc taggttaagc
     1681 agcgtttctt ttgtagcttt gacaatttcc cggggcgatt tttcatcgac tttcatgttg
     1741 tactctctca ctatgttgaa aagggatgct atggcatcgg cggtattaaa atcatcatcc
     1801 atggcttcta caaattcccg ttcatatttt tccagcttgt ctaggtattc cttgtctttt
     1861 tccacaagtt ctctatcttc tgccaggttc tccaagtgct ccatgctgta taaggcgttg
     1921 tacaatctct caagagagct ttttgcctgc tgcattaaat cctcgctaaa attaatggga
     1981 ctcctgtaat gggaagaaag cataaaaaac ctcaatactt cagggtcgta tttttttagt
     2041 atatcccgaa ccgtgaaaaa gttgccaagg gatttggaca ttttttgatt gtcgatgttt
     2101 aggtacccta catgcatgaa ataccgtgca aagggccttc cggtagctgc ttcactttgg
     2161 gcgatttcat tttcatggtg ggggaaaatg agatccgggc caccgccatg aatgtcgatg
     2221 gtttcgccca agtacttcat ggccattacc gagcactcga tgtgccatcc gggacggcct
     2281 tttccccaag ggctgtccca ggcaggttct ccgggtttct gggccttcca gagggcaaaa
     2341 tccattgggt cctttttctt ttcgccgggc tcgattcggg cccccgcctc caattcgtca
     2401 acattttgct gtgaaagctt gccgtattcc ttgaatttcc tggttgcata gtatacgtct
     2461 ccgtccactt cataggcgta gccattgtct atcaaagtct ttattagctc aataatatct
     2521 tcaatgtgct ctgtcgcctt cggatgtaca tctgccctct ctatcccaag ggcatcggca
     2581 tctttaaagt attcatctat aaagcgctca ccgagctcct ttaccgttat attttcctca
     2641 ttggcccttt ttatcatctt gtcgtcaata tcggtgaagt tttggacaaa ggtaacgtca
     2701 taaccccgaa atttaaggta atttctgatc atgtcgaagg ttataaagac cctagcattg
     2761 cctacgtgga aaaagtcgta aaccgtcggg ccacatgtgt aaatattgac cttgttgccc
     2821 tttaagggaa caaattcttc cttttttcgc gtcagtgtat tgtatatttg cattaatact
     2881 ccctccactt attatttaag tataagtagc cgtaaaacac aagagccgtt tcattggcta
     2941 cccgtaaaga tacgctcacg gctatactgg ttatgaaagt aactcgtaag aacattcttt
     3001 ggtaaaatat gacggcaaac gaaactttaa gcaagaccgt tgttattctt gagcttgaga
     3061 caaacgaagc aggagcagcg cgcaggatgc gcgctgacgg gcacttggca ggatgccaaa
     3121 ttgcccgtga acttctgcga agtgcaggct cgagctctag aataacaggg cttgcttacg
     3181 ttgaggtgcc gtcatattta caaagtagag ttttttcaaa agcaaccatc actatattga
     3241 cacaggagga tgaatatttt gtacccgtcc cgaaaaattc atcctgtttt ggatttgagt
     3301 tccttttcca agaaggcaat tctcatctct aaggtttcga tggtcttctg caggctcctc
     3361 atcatatccg ccaccgggtc ggggagcagg tgatggtcca agtccacctt ggtaaaatcc
     3421 gcatcggaaa agtccacttt tttccgaact actgccttgc cgggtattcc caccaccgta
     3481 gaattgggcg ggacatcttt aagcactacg gcacctgccc ctatcttggc attgtcgccg
     3541 atttttaccg ggccaagaat tttcgcacct gagcccacta cgatgttgtc gcccaaggtg
     3601 ggatgtcgtt ttcccttctc ttttccggtg ccacctaatg tcacgccttg gtacagcgtc
     3661 acattgtcgc ctatttctgt tgtttcgcct atgaccacac ccatgccgtg gtcgatgaaa
     3721 aaccctttgc cgatttttgc gccggggtga atttcaattc cagtcaaaaa acggtttatt
     3781 tgagagataa tccgggctat caagaccaag ttcaacttgt agaagctgtg ggctatacgg
     3841 tgcagtaaaa ttgcatgaaa gcctggataa caaagtatta cctccaatac gctctttgct
     3901 gccgggtcgc gctcgaaaat cacctttatg tcctgtctta tattgctaaa cattgttctc
     3961 cctccttctc gcttatatca aaaaaccctc gcctcatcag aggcgaggga cacttcgcgt
     4021 ttccactctg ttttataact cattgagata acggcagggc cgcataacct ctacttaatt
     4081 tcaaggtatg agctccgagg tgcaatttca gtcttgtctt acctaaaacc gcttccagcc
     4141 ggtgacggtt tctctctgga ggcagtgtca agcctacgag cctcttcaca gcttcactgt
     4201 ttttagttta ttataatact attaaaatat tcatccaaag tcaagggtct aattcattca
     4261 tatgccacta tctgcatcat cacatctaca acgccggctt ctatcattcc cacttctttt
     4321 gccgctgccc tggataaatc tatgtgacgg ccttgtatat atggccccct gtcgtttata
     4381 gtcacaaggg tgcttttgcc gttttccaaa ttggtgacaa gcaccttggt cccaaagggc
     4441 agttctctgt gggccgctgt cagtgcattt tggtcaaaga tttcaccgtt ggcggttaat
     4501 tttccgtgaa agttgggtcc gtaccaagag gccttgccta tgaatatttt cgtaccaact
     4561 ctagtatcta ctttagtatc cagcgtgttt ttcgtatatg gtttgactcg cagtgaagga
     4621 tgtgcctcct ggaaattatg gtatacaggt ttggcgtgca aagtattttg tcccccttgt
     4681 atcggcccca aacagcaaaa cactgccagc aacgccatta ctctggctat tttgcccatg
     4741 gccaccctcc tttccacgcc tccgaggtta gctgacgggt tagggtcgaa aggtataacc
     4801 cctctccaaa aaggagattc accccaagtt tggttccccc gtatccgttt attaggaatt
     4861 cggcgtttta tttaataaaa aaagtgagga tttccttatc ctcactgaat tattatgtcc
     4921 aagcatcgtg tttttattca atctaaaaat ttctctatta agcacacagc atgggctgaa
     4981 atgccttctt ctcttccctc aaaacctaag ccctcagtag ttgtcgcttt tatgctgatt
     5041 tgctctactg caatgcccaa agtatctgct atattcctgc gcatttcatc tatgtaagga
     5101 gcgagtttgg gcctttgtgc acatatgaca gaatcgacgt taattaccct gtagccccta
     5161 tcttttaata aatctttgac cttttttaac aatattatgc tgtcgatgtc cttatattcc
     5221 ggatccgtat cggggaagtg cttcccgata tcgcccaagg cgcacgcgcc tagaagggca
     5281 tcgtttatag catggacgag gacatcggca tccgaatggc ccaacaagcc tttttcaaag
     5341 ggaattacaa ctccacccag taccagtttc cttcccagca ccagtttgtg gacatcaaaa
     5401 cctatgccta ttctcatgtc tacctcctgt aattgccgta ccccacacta ataaatgcct
     5461 cggctaaaac tatgtcctct ggtgtagtaa ttttaatgtt ctcataggct ccctcgataa
     5521 ttttaacggg aaagcccaag cgctccacca agaccgtatc gtcggtcc
//
LOCUS       DAONUO010000117         5548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10784_length_5548_cov_4.466412, whole genome shotgun sequence.
ACCESSION   DAONUO010000117 DAONUO010000000
VERSION     DAONUO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10784_length_5548_cov_4.466412"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      249..376
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            441..1010
                     /locus_tag="PK768_05440"
     CDS             441..1010
                     /locus_tag="PK768_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQA60446.1"
                     /translation="MKNGVKNLVRITMLSALAVVLMFLIRFPLIPAAPFLEYDPGDVP
                     ALIAAFLFGPGAGVVVTLMVSLIQAMTVSAGSGWIGGLMHFVATGTMVTVAGIIYKRI
                     HTFRGAIIALVVSSIAMTLVMIPLNLIFTTKFMNVPVDVVKTMLVPVIIPFNLIKAIL
                     NSALTVFVYKPVGRFLRVDMGLSKSSQEV"
     misc_binding    1077..1376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1574..2923
                     /locus_tag="PK768_05445"
     CDS             1574..2923
                     /locus_tag="PK768_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HQA60447.1"
                     /translation="MKETKILLTEKEIPTHWYNIQADMPNPLQPPLSPATGKPATPDE
                     LGAIFPEEIIKQEASTERFIEIPEEVRSLYALWRPTPVYRAYRLERALDTPARIYFKH
                     EGVSPAGSHKLNTAIPQAYYNKMQGIKRLATETGAGQWGTALSMACKFFGIDCTVYMV
                     KISYNQKPYRRSMMELFGAEVFASPSDKTEAGREVLKKDPDSNGSLGIAISEAVEDAA
                     KNPDTNYSLGSVLNHVVLHQTVIGLEAKKQMEKAGYYPEVVIACSGGGSNFGGLAMPF
                     VQDKIKEGKKTRIVAAEPAACPSLTKGKFTYDYGDVAHLTPKMMMYTLGSDFMPPGIH
                     AGGLRYHGSSPIQSQLLHDGLIEAVAYEQQDVFEAAVLFAQSEGIVPAPESSHAVKAA
                     IDEALKAREAGESKTILFGLSGHGYFDMTAFDSFLSGKLQNSRLPEDAIKKALDKLS"
     gene            2952..3464
                     /locus_tag="PK768_05450"
     CDS             2952..3464
                     /locus_tag="PK768_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HQA60448.1"
                     /translation="MKLKDMILTSLLIAIGLVLHYVVPPIVGGMKPDFLLAMLFVALY
                     IDHSPKNALLAGLLAGTFSALTTGFPGGQVANMVDKFVTAFAVMAMIKAFQSFNSNLT
                     VLFTAFVGTVISGVVFLQTALFVVGSLPMAFSVLFTSIVIPAALINTAVTFICYKVVF
                     STKSMITHKA"
     gene            complement(3515..5407)
                     /locus_tag="PK768_05455"
     CDS             complement(3515..5407)
                     /locus_tag="PK768_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HQA60449.1"
                     /translation="MHEFVSFMRTLPVLARKVKTVTGTVNIGDYALLPDKIIVSGVLN
                     WQLFYIGDDDLTHFASGEIPFSTFIPVNGAAEGMGASLSGEIESIEAVISQEGSNVKI
                     QAVIRIIAVIQDKIVVSPAPGGTTDIVANTVLNENSLQTFVEEVINLNRNAKKIVAVD
                     AITSPESIEILEDKVLVQGRLSLGISYVGEDDISYFESFEIAIGGFIEMPGALPGMKA
                     CVSSSLAASQSALLSPDRLLFKAVVDWSLRLLDTGIVQITPAATGDEFEVLFMVGEEV
                     DLQHIVSAQTPLEREALKVREVKSSITELGAEIVPDKVIISGILSKTIYYVGTDGINY
                     SQQESFSFSTFADYPGALPGHEAFIDAEIEYEHFELTDSQLQHKTLLQLLIRVAEPGM
                     TPIELGDGPDVLLPRIVGQNSVQLLLSRDKKVKEKITVTKALIVLTSQIEGTGQIQII
                     RRSNAVPPAASIIDSRVETENVTAIPLENFVEVRGEVVSTITYMGTDDKTHELENRGT
                     FSTIVPVPGALPTDSVSVSSNASYYDIDIIFSGSKILEVIDVNTKVEIERQEIQEVVT
                     NVTGPHIITERVLVRVQIPSGEIVEMYVVTEVWGPKITVVKKSMLLEVVGEGPKQLDV
                     VVDVFL"
BASE COUNT         1446 a         1314 c         1303 g         1485 t
ORIGIN      
        1 gtattgctgt ttacttgttt ttttgttctt cacttccttc ttttggaaaa aggttatggt
       61 ttattggttt ttttgttggg tttgttagtt ttttataggg aattattaac tgtttccgaa
      121 actattcttt ataattatct atagtatatt ttaccaaaca agaaaaaata cacaaccaaa
      181 accaggtaaa caaaacattg acatttgcac tttttagtgc tattatatgg ataacagaat
      241 acgccagata taatcttcag ggcagggtga aattcccgac cggcggtaat gcgcaatggc
      301 gctagcccgc gagcgcaagc tgacttcggt gagattccga ggccgacagt taaagtctgg
      361 atgggagaag attatttttt gatgccttcg ttccctggga ttatatcttg gggaattttt
      421 atttatggag gtagtgctaa atgaaaaacg gagtcaaaaa ccttgtaaga atcacaatgc
      481 tttcagccct agcagtggta ttgatgtttt tgatcaggtt ccccttgatc ccggcagcgc
      541 catttttgga atatgatccg ggagatgtgc ctgccttaat agccgcgttt ttgttcgggc
      601 cgggtgccgg tgttgtcgtc accttaatgg tatccctaat ccaggccatg accgtcagcg
      661 caggcagcgg ctggataggg ggtcttatgc attttgttgc gacaggcacc atggtaactg
      721 ttgcaggcat catctacaag agaatccaca ccttcagggg agcaataatt gcattggtgg
      781 taagttccat tgccatgacc cttgtgatga tacccctcaa ccttattttc accacaaaat
      841 ttatgaatgt gcccgtggat gtggtaaaga ccatgctcgt tccggtcatt atccccttta
      901 acctgataaa ggcaatcctc aattcggccc tgacggtttt tgtctacaag ccggttggca
      961 ggttcctgag ggtggatatg ggcctcagca agagcagcca ggaagtttaa agaaaaaagt
     1021 tctaaaaaaa gttgacacct gctttggaat ggtgtattat atacggtaac aaattgagaa
     1081 attccgtgaa ggggaagagt aatctagacg aaaggtcaaa gagagccgcc gctggtggga
     1141 gtgcggtacc ggaagtcttg atgaatgggc cccagagaag gtgcggcaaa attacagtag
     1201 ctgcacacgg caagtccccc gttagcagag gacaggatat cgcgatatca tcgccgtatc
     1261 cggaagagtg agtattacat tgaggtggca aagcatttta tcctgaagat aaaatgcttt
     1321 tttgttacca agtgtaagac taatttgggt ggcaccgcgg aacaaccgtc ccttaactgg
     1381 gacggttttt ttgttttaat tgattctctc aggctcaaag ggaaagttct tctttgacgc
     1441 caaagaaagg gagatgcggg gtgatagaaa tgacatgaaa gcggacaatt gaaatataca
     1501 acagcttgaa ggctttgcga aaaggaaatc ttaaaaggct agtgagaaaa tttttaagca
     1561 aggagagatt gagatgaagg aaacaaaaat tttgttgact gaaaaagaaa ttcctaccca
     1621 ttggtataat attcaggctg acatgccaaa tcccctacaa cctcccctga gccctgcgac
     1681 gggaaagccg gctacccccg atgaactggg agcaattttc ccggaggaaa ttataaagca
     1741 ggaagcgagc acagagcgct ttatcgaaat acccgaggaa gtaagaagtc tttatgccct
     1801 gtggaggccc acacccgtgt atagggcata tcgcctggaa agggcattgg atacgccggc
     1861 aagaatctac tttaagcatg aaggcgtaag ccctgccggc agccacaagt taaataccgc
     1921 cataccccag gcctattaca acaaaatgca gggaattaag cggctggcca ccgagaccgg
     1981 tgcgggccag tggggaactg ccctttccat ggcctgtaag ttctttggca tagactgcac
     2041 cgtctacatg gttaagataa gctacaatca aaagccctat cgccgttcga tgatggagct
     2101 tttcggggca gaggtctttg caagccccag cgacaagacc gaagccggcc gcgaagtgct
     2161 gaaaaaagac ccggattcca acggcagcct tggcattgcc ataagcgaag cggtggaaga
     2221 tgcggccaaa aatccggata ccaattattc cttgggcagc gtattgaacc acgtggtgct
     2281 gcatcagacc gtgataggcc ttgaggcgaa aaagcagatg gaaaaggccg gctactatcc
     2341 ggaagtggtc attgcctgtt ccggcggcgg cagcaatttt ggcggccttg ccatgccctt
     2401 cgtacaggac aaaatcaagg aaggcaaaaa gacccgcatc gtcgctgcag agcctgctgc
     2461 ctgtcccagc ttgacgaaag gcaaattcac ctacgactac ggcgacgtgg cccatctgac
     2521 tccgaagatg atgatgtaca ctttgggaag cgactttatg cccccgggca ttcacgcggg
     2581 aggcctcaga tatcacggct catccccaat ccagagccag ctcttgcacg acggcctgat
     2641 tgaagcagta gcctatgagc agcaggacgt ctttgaggca gccgtgctct ttgcccaaag
     2701 tgaaggcatc gtgccggcac cggaatcatc ccatgcggtg aaggctgcca ttgacgaagc
     2761 tttaaaagct agagaagccg gcgagagcaa gaccattctc tttggcttaa gcggccacgg
     2821 atactttgac atgacagcat ttgacagttt tctttcaggc aaactgcaaa attcccgttt
     2881 gcctgaggat gctattaaaa aagctctcga taaactgtcg taaagtgaaa aaataacgga
     2941 gggaaagaac gatgaaactt aaggatatga ttttgacatc actgctgata gctataggct
     3001 tggtgcttca ctatgtagtt ccacccattg tgggtggcat gaaaccggat ttcttgctgg
     3061 cgatgctctt tgtggccctt tatatagacc atagccccaa gaatgccttg cttgcaggac
     3121 tgctcgccgg gacattctca gcccttacaa cgggctttcc cggcggccag gttgccaaca
     3181 tggttgacaa attcgtcacg gcctttgcgg taatggccat gataaaggcg tttcagagct
     3241 ttaattccaa cctgaccgtc ctgtttacgg cctttgtcgg caccgtgata agcggcgtgg
     3301 tctttttaca gacggcccta ttcgtagttg gaagcctgcc catggccttt tcggtgctct
     3361 ttaccagcat agtcattccc gcagccctga taaacaccgc tgtcacattc atatgttaca
     3421 aagtggtgtt ctctacaaaa agcatgatta ctcacaaggc ctgaaagtct tgaagctgtc
     3481 gcaagctgaa tctagtttgc ggcagctttt atattcacaa aaatacgtcc acaaccacat
     3541 ccaactgctt cggcccttcg cccaccactt ccaaaagcat gctcttcttt acgacagtaa
     3601 tttttggtcc ccaaacctct gtcactacgt acatttctac gatttcgcct gaaggtattt
     3661 gaacccttac caggactctt tcagtgataa tgtgggggcc tgttacattg gtaaccactt
     3721 cctggatttc ctgccgctca atttctactt tagtattgac gtcgatgact tccagaatct
     3781 tgctgcccga gaagattatg tcaatgtcgt aataacttgc gttgctgctg acggaaaccg
     3841 aatccgtcgg aagagctccc ggcaccggga caatggtgga aaaagtccct cggttttcaa
     3901 gctcatgggt tttatcgtcg gtgcccatgt aggtaattgt gctgacaact tcccccctta
     3961 cctcaacgaa attttccaag ggaatagcag taacattttc cgtttcaact cgactgtcga
     4021 tgatggatgc ggcaggagga acggcattgg acctgcggat gatctggatc tggccagttc
     4081 cttctatttg cgaagttaac actatgaggg cctttgttac ggttattttc tccttgactt
     4141 ttttatcccg gctgagcagc agctggacag aattttgccc tacaattctt ggcagcagca
     4201 catcaggccc atcacccaat tcgatgggcg tcattccagg ctctgctacc cttatcaaaa
     4261 gctgtagcag cgtcttgtgc tgaagctgag aatcagtcag ctcgaaatgt tcgtattcaa
     4321 tttctgcatc gataaaggcc tcgtggccgg gcagggctcc cgggtagtct gcaaaggtag
     4381 aaaaactaaa actttcctgc tggctgtaat tgatgccatc cgttcccacg taatatattg
     4441 ttttggacag gataccgctg attatgactt tgtcgggaac gatttctgcc ccaagttccg
     4501 ttatgctgct tttaacttcc cttaccttaa gcgcctccct ctccaacggc gtttgggcac
     4561 tcactatgtg ctgaaggtca acttcctctc ctaccatgaa aaggacttca aactcatctc
     4621 cggtagctgc aggtgtaatc tgtacaatcc cggtatctaa aagccttaga ctccagtcaa
     4681 ccacagcttt gaataaaagc cggtctggag aaagcagggc agattgactt gccgctaagc
     4741 tcgaggatac gcatgccttc attcccggca aggctcccgg catttcgatg aaaccgccta
     4801 ttgcaatttc aaagctttca aaatagctta tgtcatcttc ccctacgtag cttatcccca
     4861 gcgagagtcg gccctggacc agcaccttgt cttcaagaat ttcaatgctt tccggtgatg
     4921 ttattgcatc tactgctact atctttttag cgtttcgatt gagatttatc acttcctcta
     4981 caaaggtctg aagggaattt tcatttagaa cggtattcgc aactatgtcg gtggtacctc
     5041 cgggtgccgg tgaaactacg attttatctt ggattacagc tattattctt atgaccgctt
     5101 gtatctttac attagatcct tcctgcgata taacggcctc aatactttct atctctcctg
     5161 acaaactggc acccatgcct tctgcagcac cgtttacggg gatgaaggtt gaaaagggta
     5221 tttcccctga agcaaagtgc gtcagatcgt catcgccgat ataaaatagc tgccagttta
     5281 aaactcccga gacaattatt ttatcaggca aaagggcata atcacctata tttacggtgc
     5341 cggtcaccgt cttgactttt ctagccagta caggaagggt tctcataaaa cttacaaact
     5401 catgcacaat aatccctcct ttaatagctg tcatttcata atattccgca gggctttgtt
     5461 ttgacagtgc ctcctgtatt tttgaccatc ccccttcata tttccaaaac acgaatcata
     5521 aaaatttaca aaggaggggg ctggcaat
//
LOCUS       DAONUO010000118         5541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10800_length_5541_cov_3.997995, whole genome shotgun sequence.
ACCESSION   DAONUO010000118 DAONUO010000000
VERSION     DAONUO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10800_length_5541_cov_3.997995"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK768_05460"
     CDS             <1..182
                     /locus_tag="PK768_05460"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HQA60450.1"
                     /translation="TIPLGTEIFIEDMGLFVAEDTGGNIKNNRLDIYFDCYDEAVSFG
                     VRLVKVWVQHPGIVR"
     gene            259..582
                     /locus_tag="PK768_05465"
     CDS             259..582
                     /locus_tag="PK768_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60451.1"
                     /translation="MDMDTKELETKIQEFLISKGFQFGIWGTCRHSTGIDFHCALVRP
                     KYSPITGGSYTLAVDPITVKGWVIRPPYSYTEWQCIWQELYSTPEELVAILEKRISPP
                     TGCWG"
     gene            703..2628
                     /gene="ligA"
                     /locus_tag="PK768_05470"
     CDS             703..2628
                     /gene="ligA"
                     /locus_tag="PK768_05470"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQA60452.1"
                     /translation="MSRINELREQLSLFDEAYEQGKPLISDTEYEKMYSELQKLEKKA
                     GDVSQTSPTQTIPFFKVDGLETVKHTVPMLSLDKTNRYDILRKWYSQAKDKVLVQPKL
                     DGLTIVLRYKNGNLVDAITRGNGLEGERVLHTISIIENLPKKIPFKGYLEVRGEAYIP
                     FSEFNRINNGEYSNPRAMATGMVRRHDSSQVQGLRITIFDLITAEGQKFTSDYDQILF
                     LIKQGFKVVDTNRCDYLDRVIQICMQYQETLRVELDYPIDGMVVKFDSLELRERLGAT
                     SKSPRWAMAFKFPSLEEFTTLKEVIWQVGKSGLITPVAIFDPIEIDGVVINRATLHNK
                     QFILDQDIRIGDRIVVARANDVIPKVVRAAVEARTKELNLPEIPTKCPSCGEDIWIQP
                     DVPHITCLNPFCREQMIEKIIHFCSRDAMDIEGMGEVNVQTLFDLGFIQNPADLYDLH
                     NKAEEIEKLEGFGKVKVKKILEGIEKSKDLPLSRLLYALAIPGVGKTTAQAIVKKFRT
                     IESLKNASREELASVDGIGPVTAQGIWFCFINPQIQNMISRMENAGVNTEEMIAETKE
                     TLSGKTFVLTGTMSLPRSEIENMIKQNGGKVAGSISAKTSYLVAAQGESGTTKYQKAM
                     QLGIPIISESQLMEMLV"
     gene            2640..2876
                     /locus_tag="PK768_05475"
     CDS             2640..2876
                     /locus_tag="PK768_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HQA60453.1"
                     /translation="MTGRVIVISGTLTKKRSVFVKLIRQNGGRVSNSVTKRTSYLLLG
                     PDKKGGCKHRQALSYGVPVISESQFFQILQGGIQ"
     gene            2873..3892
                     /locus_tag="PK768_05480"
     CDS             2873..3892
                     /locus_tag="PK768_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HQA60454.1"
                     /translation="MSLLLIDGSSILNSCFFGTVPQEYYRAKTPEEKRFILPNIMQTK
                     TGIFTNGVYAMCKSLKNLIERCKPQYLAVAWDVTRDTFRRELYPDYKANRAETAPELK
                     SQFAIMQDVLRAAGIKQFMDSNIEADDFIGSLSRQFESQTPVYIYTKDQDVLQLVSDR
                     TRVWLITNKAEDLKRIYGKPEGFVPNSAFEFTPLLVKEVYGLYPEQIIDKKALEGDSS
                     DNIPGVRGVGEKAAVPLLQEYGTVEGIYDALEDEKHFKDVCKDLGIRSPIKALTEGRE
                     LAYLSKTLATIKICSLPVSLEELKLQIDRYSLDAKFRELEFTSLVSMPAVPQKVEIQV
                     QGALF"
     gene            3936..4109
                     /locus_tag="PK768_05485"
     CDS             3936..4109
                     /locus_tag="PK768_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60455.1"
                     /translation="MSEAELIYDDVNDVGEELAWLDYLTKERAGIMKDRERRLVNLKG
                     KFVVVCDGKNQPL"
     gene            4543..5079
                     /locus_tag="PK768_05490"
     CDS             4543..5079
                     /locus_tag="PK768_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60456.1"
                     /translation="MAGRIEVITGPMYSGKTTDLLRRLERYVLAGKKFRAFKPGKDTR
                     QQRTSISTIRGEIDLSPTPVASLAELAGLPTEIEVVALDEAQFFGEEVVAFCQHAKDQ
                     GKTVLVSGLDMDFNRKPFGHMGELMAIADSVTKMTAICGCGNDAAYTKKISGDKSQQV
                     EIGDQCYIPCCARCYGAT"
     gene            5278..5490
                     /locus_tag="PK768_05495"
     CDS             5278..5490
                     /locus_tag="PK768_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60457.1"
                     /translation="MKKKSIATQKDDIVTIPGKQQNVRLAFVGGDGLSAGYKLLAELF
                     IDQYIRDGQIITGNKPIEPLIQENVK"
BASE COUNT         1737 a         1041 c         1307 g         1456 t
ORIGIN      
        1 gaacaatccc tttaggtacg gaaatattta tcgaggacat gggactattt gtggcagaag
       61 atactggggg aaatataaaa aacaaccggt tggacatata ttttgattgc tatgatgaag
      121 cagtcagttt tggagtccgt ctggttaaag tctgggtgca acacccaggc atagtaagat
      181 aatctaagcg acctataggt cgctttttta tactttaaaa attacaaaat cccatttagt
      241 tggggaaggg aggattttat ggatatggat acaaaagaat tagaaaccaa aatccaggaa
      301 tttctaattt ccaagggatt tcaattcggt atttggggta cctgccgtca ttctaccggc
      361 attgactttc attgtgcctt ggtcagaccg aagtattcac ctattactgg tggatcatac
      421 actttagcgg tcgatccaat aacggtaaag ggatgggtga tcagaccccc atattcctac
      481 actgaatggc agtgtatatg gcaggaattg tacagcaccc cagaagaact ggttgcgatt
      541 ctcgaaaaga gaatttctcc gcctaccggt tgttggggat aggtattttg aagaacggta
      601 tatttattgc aggtatattt caccttccaa agcggcttaa agccgctttt tttataccca
      661 aaataacaaa aatcccatta agttggggaa ggaggttggt atatgagcag aattaacgaa
      721 cttcgtgaac aattgtccct gttcgatgaa gcctacgaac agggaaagcc tctgatttca
      781 gatacggaat acgaaaaaat gtattctgaa ctccaaaaat tagagaaaaa ggccggggat
      841 gtaagtcaaa cttcaccaac ccaaaccatc ccgtttttca aagtagatgg tctggaaacc
      901 gtgaagcata ctgtccccat gttgtcgcta gacaaaacaa accgatacga cattctaagg
      961 aaatggtatt cgcaagctaa agataaagtg cttgtgcagc ccaagctgga cggattgact
     1021 attgtactgc gatataaaaa tggcaatctc gtcgatgcta taacccgagg caatggcctg
     1081 gaaggtgagc gagtattgca caccatcagt attatcgaga acttacctaa gaagatacct
     1141 tttaagggct atcttgaggt aaggggagag gcttatattc ccttctccga gttcaaccgg
     1201 ataaataacg gggaatattc caatcctaga gcaatggcga ctggaatggt aaggcgccat
     1261 gattcttctc aagtacaagg tttaaggata accatatttg atctgattac cgcagaaggc
     1321 cagaaattta cttccgacta cgaccagata ttattcctta ttaaacaagg atttaaagta
     1381 gtggatacaa acagatgtga ttatttggac cgggtaattc agatatgtat gcaatatcaa
     1441 gaaaccttgc gagttgagtt agattatcca atagacggta tggtagtgaa atttgattcc
     1501 cttgaactgc gggaaagact tggcgctaca tcaaaatctc ctcgatgggc aatggcgttt
     1561 aagtttccca gtttggagga atttactacc cttaaagaag ttatctggca ggtaggcaag
     1621 agcggcctga ttacgccggt tgcgattttt gatccaatag aaattgatgg ggtggttatt
     1681 aatcgggcca cactacacaa caagcaattt attctggatc aggacatacg cattggcgac
     1741 cggattgtag tcgcccgtgc caacgacgtt atacccaagg tagtccgtgc ggcagtcgag
     1801 gcccgtacaa aggagcttaa cctgcccgaa atacccacta aatgcccttc ctgtggtgaa
     1861 gacatatgga tacaacctga cgttccccat attacatgcc tgaatccatt ctgtagggaa
     1921 caaatgattg aaaaaatcat tcatttttgc tcccgggatg ctatggatat tgaaggcatg
     1981 ggcgaagtta atgtgcaaac attatttgac ttgggcttta tccaaaaccc tgctgactta
     2041 tatgatttac acaataaagc tgaggaaata gaaaaactcg aaggatttgg aaaagttaaa
     2101 gtcaagaaaa tacttgaagg aattgagaaa tccaaagacc tgcctttatc tcggctatta
     2161 tacgccttag ctattcccgg agtaggtaag actactgccc aggcaatagt taaaaaattc
     2221 cgcaccatag agagcttgaa aaacgcctcg cgggaggaat tggctagtgt agatggcatt
     2281 ggtccggtaa ctgctcaggg catctggttt tgttttatta atccacaaat acaaaacatg
     2341 ataagccgca tggagaatgc aggagtcaat accgaggaaa tgatagccga aaccaaagaa
     2401 accctcagcg ggaaaacctt tgtacttaca ggtactatgt ccctgccacg ttctgaaatt
     2461 gagaacatga ttaagcagaa cggcggtaaa gtagcaggta gcataagtgc taaaaccagt
     2521 tatttagtag ctgcacaagg ggaatccggc acaacaaaat accaaaaagc tatgcaattg
     2581 ggaattccta tcattagtga gtctcaatta atggagatgt tggtataagg agggtttata
     2641 tgactggtcg ggttatagta attagcggga cacttacaaa gaaacgctct gtttttgtga
     2701 agttgattcg gcagaatgga ggaagggtgt ctaattcggt aactaagaga actagctatt
     2761 tactgcttgg accggataaa aaaggtgggt gtaaacaccg tcaggctttg tcatatggcg
     2821 ttcccgtcat atccgaaagc caattctttc aaatccttca aggaggtata caatgagcct
     2881 gctacttata gatggcagca gtatattaaa ctcctgtttc ttcggtactg taccgcagga
     2941 atattaccgg gctaagacac ccgaggaaaa gagatttata ctgccgaaca tcatgcaaac
     3001 caaaacaggt attttcacta atggtgttta tgccatgtgt aaaagcctga aaaacctaat
     3061 tgagagatgt aagcctcaat atttggccgt agcctgggat gtcacccgtg atacttttcg
     3121 gcgggagtta taccctgact ataaagccaa ccgtgcagaa actgcccctg agttaaaaag
     3181 tcagtttgct atcatgcagg atgttcttcg ggctgcaggt attaaacagt tcatggactc
     3241 caatattgaa gcagacgact ttataggaag cctcagccgg cagtttgaat cacaaactcc
     3301 ggtttacatc tacactaaag atcaggatgt tctgcagctg gtttccgacc gtactagagt
     3361 atggctgata accaacaaag cagaggattt gaagcgaatc tatggcaagc cagaaggttt
     3421 tgtacccaat agcgcatttg aattcactcc gctgttggtt aaagaagtct atggattgta
     3481 tccggagcag ataattgaca aaaaagcact cgaaggagat tcatcagaca acattcctgg
     3541 agtcagagga gtaggggaga aagcggctgt tccactactt caggaatacg gtacagtaga
     3601 gggaatttac gatgccttag aggacgaaaa gcacttcaag gatgtttgca aagacttggg
     3661 aatcagatct cccataaaag ccttaacaga aggcagggaa ttagcttatt tatccaagac
     3721 tttagcaact atcaaaatat gtagtttgcc tgtatccctg gaagaactca aattgcagat
     3781 agaccggtat tcgctcgatg ctaaattccg cgaactggag tttaccagtt tagtgagtat
     3841 gccggcagtc ccccaaaaag tagaaataca ggtacaggga gctttatttt aatcaaatcc
     3901 cgtgttgggg aacgattgta aaggagtcag ataacttgag cgaagcagaa cttatctatg
     3961 atgatgtcaa cgatgttggc gaggaattag cttggctgga ttatttgact aaagagagag
     4021 caggtataat gaaagacagg gaaaggcgtt tagttaatct taaaggtaag tttgttgttg
     4081 tctgtgacgg aaaaaatcag cctttatgaa caaaaaacaa aaaatccttt ttaagaaggg
     4141 aggaagtttt cagaatccgc tttatgcggt gggttttacc cgtttcccca cctggctgag
     4201 aagcctggtt cctttccttg aattcaagga agggagggtg cgtgtagctt gttggagctc
     4261 atttttctag tcattattat tttaatctgt attgcgctgc taattctggc agtacgtgac
     4321 aagacctctt acatagaggt tttgcggctc cagttattca gcgttttcag acttgaaatg
     4381 attatgacag aaaagagaaa acctccggtg tggcggaggt cccaacaacg acggcccaaa
     4441 taaacgggcc acgtcacggt cagttccagc tgaccgtgac tctaatttga ttataagaaa
     4501 aaaatcacgc ttgtcaaagt gcgtggagaa ggaaggtgac ttatggctgg aagaatagaa
     4561 gtaattaccg ggccgatgta tagtgggaag accaccgatt tgttgcgccg gttggaaagg
     4621 tatgtgcttg ccggcaagaa atttcgtgct ttcaaacccg ggaaagacac acggcaacag
     4681 aggacatcta tttcaaccat caggggggaa atagatttat ctccaactcc agtagcttca
     4741 ctagcagagc ttgcaggatt acctacagaa atagaggttg tcgctttaga cgaagcccaa
     4801 ttttttggag aggaagtcgt agctttttgt caacacgcta aagatcaggg aaaaactgtt
     4861 ctagtaagcg gcctggatat ggattttaac agaaagccct ttggacatat gggcgagtta
     4921 atggctattg ccgatagtgt cactaaaatg actgcaatat gtggctgcgg caatgatgca
     4981 gcctatacca aaaagataag cggtgataaa agccagcagg tagaaatagg cgatcaatgt
     5041 tatattccat gctgcgccag gtgttatggt gcaacttgaa gatgaattta gcatggaacc
     5101 ggatatagtt acgtagcccc ctatttaggg ggctttttta tggacaaata atataaaaat
     5161 ccttaatgct tgtatgatgg cctaagccgg ggttgcccgg ctttttccat tttctgctct
     5221 aatatgacaa gcctacaaat tcataattat tttgcaaaaa ggaaggagtt gaaggagttg
     5281 aaaaagaaaa gtattgctac gcaaaaagat gatattgtaa ccatcccagg taaacagcaa
     5341 aatgttcgat tagcctttgt aggtggggat ggcctcagtg caggatacaa actattagcc
     5401 gaattattta tagaccagta tatacgggat ggccaaataa ttactggtaa caagccaata
     5461 gaaccgctaa ttcaggagaa tgtcaaataa aaagagaggt ataaaatgcg tgcggcagtt
     5521 tatgcaagag taagtacaga a
//
LOCUS       DAONUO010000119         5511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10865_length_5511_cov_3.385630, whole genome shotgun sequence.
ACCESSION   DAONUO010000119 DAONUO010000000
VERSION     DAONUO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10865_length_5511_cov_3.385630"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..781)
                     /locus_tag="PK768_05500"
     CDS             complement(209..781)
                     /locus_tag="PK768_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HQA60458.1"
                     /translation="MWYYVKKLQYPANVSRTDLGMAKFLYAQYGGPDSELSAAVRYLT
                     QRYTMPIPQAKATLTDIGTEELAHWEIIGTLIYKLTDGATPEQMRRAGLGDHFTQHDN
                     ALYPHDAAGVPWTAAYIQATGDPITDLYENMAAEQKARTTYEHLISLTDDPGVIATLS
                     FLRQREVVHFQRFGETLMLVREYMNKKHVF"
     gene            complement(794..1048)
                     /locus_tag="PK768_05505"
     CDS             complement(794..1048)
                     /locus_tag="PK768_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HQA60459.1"
                     /translation="MYSKDMVDLLKEIMAVDFVVYELALFLNTHPTDRRALEDHNNFA
                     RRSQELRAMYEKKYGPLTLGSVSEFPYQYINNPWPWEIRY"
     gene            complement(1068..1244)
                     /locus_tag="PK768_05510"
     CDS             complement(1068..1244)
                     /locus_tag="PK768_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HQA60460.1"
                     /translation="MSNPENDIKTQSLFSDLRLAEAYIPFQRYGQIFDPMAALMNGTL
                     FPELYRPYRKRPEL"
     gene            1511..3010
                     /locus_tag="PK768_05515"
     CDS             1511..3010
                     /locus_tag="PK768_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQA60461.1"
                     /translation="MESKELVNNKLIRGLGLKEAITITVGTVVGVGLFTVGANGVGYL
                     GPSIILATLAAFIFSIYPALLYAEMGAMLPFAGGTYNYAKYGVGKAWGFLAGWNFVIS
                     LISVVSGEALAFSNYFKWMFEGLGITIPVDERVIASLIIILFIVINYRGIEIAGKWQN
                     AFMFFFWGVSLIWFFIMIPHINIHSHAPFVGSVSADWREFIFVTSLVWWCFAGFETAC
                     AMGEEIQYPQINIPRAMFLAPFIIFVVNAVFQWFLLGIVPPSNLVLIQESAAPYAEAM
                     KVAGIVGIPMVILCAGIAFGGDFSTLNAGVAAPARYLFSMSRDGSLPPVFSKLHPKYK
                     TPYIAVLFLGAITLLFVSTGSLIYIASLSLFADLFYYIIGFMGSIGLRVKQPQLQRPY
                     KAPMLKVGATISIIVYIIMTTQLPKDAVITGVLWSVVGLILYRFWSRAHSDNDVHSSD
                     DAVLKQELPELPTGMELERLNREYSLWQKIVGFAFLLSIMLYVIPYFFN"
     gene            3090..4346
                     /gene="hisD"
                     /locus_tag="PK768_05520"
     CDS             3090..4346
                     /gene="hisD"
                     /locus_tag="PK768_05520"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQA60462.1"
                     /translation="MKIIKDAKKGKMQEVDVRQKVFNILEDVRKNGDQALIEYNKKFD
                     DYTGNMRITADDIKRAYDSLDEQVIKDIRFAAERIRRFAEIQKDSMREVKEVVSPGVI
                     LGHRIIPVQSCATYIPGGRYPLPSSALMSIIPAKVANVPRIVACSPPDSDVGSINPAT
                     LVAMDIAGADEIYCMGGAHAIGALAYGTETIKPVDMIVGPGNIWVTEAKRQVFGTVGI
                     DFLAGPSEVLIIADETAEPDFIASDLLAQSEHDPNARGILISLSEDLAHRVISSLEKL
                     LADLETEEVARKSWQDNGQVIVVDSVEDAVKLANKIAPEHLELCINNPEDVVDLLLNY
                     GSLFIGNYAAEVFGDYVSGTNHILPTMKAARYTGGVWVGTFLKVVSFQMISKEGAKEL
                     IPAASSLAKTEGLYAHKLAADIRLWD"
     gene            complement(4686..>5511)
                     /gene="cysK"
                     /locus_tag="PK768_05525"
     CDS             complement(4686..>5511)
                     /gene="cysK"
                     /locus_tag="PK768_05525"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQA60463.1"
                     /translation="VAKLESFNPGGSVKDRIAFSMIQDAEDRGILKPGSTVIEPTSGN
                     TGIGLAMVCAVKGYRLILVMPESMSVERRKLLKAYGAEFVLTPAEKGMKGAVEKAEEL
                     AKTQGYFMPLQFDNPANPAIHERTTAQEILKQTGGKLDAFVSGIGTGGTFTGVARTIK
                     AKLPHVKFVAVEPEASPVLSGGKPSPHKIQGIGAGFIPKVLDTDLIDQIITVPNEEAY
                     NFARLLATKEGILAGISSGAAVYAAQKLAKDLGPGKLVVAVLPDTGERYLSVEGLF"
BASE COUNT         1507 a         1125 c         1235 g         1644 t
ORIGIN      
        1 gatacgggcc cgtatgtccg gtggtgtgag gggacggcga gtaaaataat tactcacctc
       61 ctactcgatt gatgccctcc tttatctatc ttatacgttg taatattatc tactttcttg
      121 atgcttcatt ttcaatccga actagctttt ctaggataca gaagcatcta taaaagaaat
      181 cgccgattgt taaaagcatt tgagattatc aaaacacgtg tttcttattc atgtattccc
      241 tgaccagcat cagcgtttca ccgaaacgct gaaaatgcac tacttctcgc tgccttaaaa
      301 atgaaagtgt agcgattaca ccggggtcat cggttaaact tatgagatgt tcgtaggtag
      361 tcctggcttt ttgctccgcg gccatatttt cgtaaaggtc ggttatggga tcccccgttg
      421 cctgaatgta cgccgctgtc catggtactc cagctgcatc gtgagggtaa agggcattgt
      481 cgtgctgtgt gaaatggtcg cctaagcctg ccctcctcat ttgttcaggc gtcgccccat
      541 cggtcaattt atatatcaat gtcccaatta tctcccagtg agcaagttct tcagttccaa
      601 tatccgtcaa agtagctttc gcctgtggta tcggcatggt atagcgctgt gttaaatatc
      661 ttacggctgc agatagctcg ctatcagggc caccatactg tgcatataaa aacttggcca
      721 tgcccaagtc agtccgcgat acattagccg gatactgcaa ctttttcacg tagtaccaca
      781 tacaagttcc tcctcaatac cttatttccc atggccatgg attgtttata tattggtacg
      841 gaaactcaga aacggaaccc aacgtcagag gtccgtattt tttttcgtac atggctctaa
      901 gctcctgaga ccttcttgca aaattattat ggtcttccaa ggcccttcgg tcagtaggat
      961 gagtattcaa aaacagggcc agttcatata ccacaaaatc cacagccatg atttctttca
     1021 acaggtccac catatctttt gaatacacaa aaatcacctc tttcagccta tagctcgggt
     1081 ctttttctat aaggtctata aagctcaggg aatagcgtcc cgttcatcaa tgccgccata
     1141 ggatcaaaaa tttgaccgta tctttggaat ggaatatatg cttcagccag cctaaggtca
     1201 gaaaacaagc tttgtgtctt gatgtcgttt tcaggatttg acatagcaac ccctcctaaa
     1261 aggtgataaa gcatgataaa tctattgttt tactagtata ttatgtcagt aataaatttt
     1321 cggtgctaaa ctgtgggaaa tatggtaaaa tgtaaagtga attctttatc aagttaccat
     1381 gttaaaacgt taatacgtta ttagcttata aaacatgcat cattagtcct gtaaatcgga
     1441 ataattaaat ggagtgaaat tgtagaatca ttaattatat tataggaggt ttatataagg
     1501 aggttgcatt gtggagagta aggaacttgt taataacaag ctgataaggg ggttaggatt
     1561 aaaagaggct ataactatta ccgttggcac agtagtgggc gtcggcttgt ttaccgtagg
     1621 agccaatgga gtaggctatt tagggccatc gattatattg gcaacacttg cagcttttat
     1681 ttttagcatt tatccggccc tcctgtacgc agaaatgggc gcgatgctgc cttttgcagg
     1741 agggacgtac aattatgcga agtatggggt aggaaaggcc tggggatttt tagccggttg
     1801 gaattttgtc atttccctga tatccgttgt aagcggtgaa gctttagctt ttagcaatta
     1861 ttttaaatgg atgttcgaag gccttggaat aacgattccg gtagatgaaa gagttattgc
     1921 cagcttgata atcatacttt ttatcgttat aaattacagg ggaatagaaa tcgccggcaa
     1981 atggcagaat gctttcatgt ttttcttttg gggagtatcg ttgatatggt tctttatcat
     2041 gattccgcac ataaacatac atagccacgc gccctttgtc ggttcggtaa gtgccgactg
     2101 gagagagttt atatttgtca catccctggt atggtggtgc tttgccgggt tcgagacagc
     2161 ctgtgcaatg ggggaagaaa tccagtatcc tcaaataaat atacccagag caatgttctt
     2221 agcacccttt ataatctttg tagttaatgc agttttccag tggttcttat tgggcatcgt
     2281 cccaccttct aatttagtgc tcattcaaga atcagcagcc ccttatgctg aagcgatgaa
     2341 agtagccggc atagttggca tccccatggt aattttgtgt gcaggcatcg cgtttggtgg
     2401 tgatttttca acattaaatg ctggagtagc agctcctgct cgatacctgt ttagcatgtc
     2461 tagagacgga agtttgccac ccgtgttttc caaattacat ccgaaatata aaactcctta
     2521 tattgcagtg ctgttcctgg gtgccatcac actattgttt gtttcaaccg gttcacttat
     2581 ctacattgct tccttgagcc tatttgccga tttattttac tacattatcg gtttcatggg
     2641 ctcaataggc cttagggtta aacagcctca gttacaaagg ccatacaaag cgccgatgct
     2701 aaaagtagga gcgacgataa gtatcatagt ctacataatc atgaccactc aactgccaaa
     2761 ggatgctgtg attaccggtg tcttatggtc tgtagtcggt ttaatcttgt accgtttctg
     2821 gagcagggct catagtgaca atgatgtgca ttcaagcgac gatgctgtat tgaaacagga
     2881 attacctgaa cttcctacgg gcatggaatt ggaaaggtta aatagggaat actcattatg
     2941 gcaaaaaata gttggattcg cctttttact gtcaattatg ctatatgtca taccatactt
     3001 ttttaactaa agctaactat tagtataaaa tgggtaaagc acaccgtgtg cgaacacaaa
     3061 tgctatttta ttattggagg aaaatactca tgaagattat caaggatgcc aaaaaaggta
     3121 agatgcaaga agtggatgtc aggcagaaag tttttaacat attggaggat gttcggaaaa
     3181 atggcgacca agcattgatc gagtataata aaaagtttga tgattacacg gggaatatga
     3241 gaatcactgc cgacgacata aaaagagcat atgacagcct ggatgagcag gttataaaag
     3301 acatcaggtt tgctgcggag agaataagaa gatttgcaga aatacaaaaa gactcaatga
     3361 gagaagtaaa agaagtagtt tcaccgggtg tgatcttggg tcatcgaata attccggtcc
     3421 aatcctgcgc cacttacatt cccggaggtc gttacccgct gccgtcttcc gccctgatgt
     3481 ctattatacc ggcgaaagtg gccaatgtgc ccagaatagt tgcctgttct ccaccggaca
     3541 gtgacgtggg aagcatcaat ccggctacgt tggttgccat ggatatagcc ggtgccgatg
     3601 aaatttattg catgggaggc gcccatgcta ttggggcttt ggcctatgga acggaaacaa
     3661 taaaacccgt tgatatgata gttggcccgg gaaatatatg ggttacagaa gccaagcggc
     3721 aggtctttgg aacagtggga atagactttt tggctggtcc cagtgaagtc ctgattatcg
     3781 ccgatgaaac tgcagaaccg gattttatag catctgacct actcgctcag tcggaacatg
     3841 acccaaatgc ccgcggcata cttataagct tatctgaaga cctggcacat agggtcatat
     3901 caagtctaga aaaactgtta gccgaccttg agactgaaga ggtagcgaga aaatcctggc
     3961 aagacaatgg ccaggttatt gtagtcgatt cggtggaaga tgctgtaaaa cttgccaata
     4021 aaatagctcc ggagcatttg gaactttgca taaacaatcc agaggatgtt gttgatttat
     4081 tgctcaatta cggttcgctt tttattggca actatgccgc tgaggttttc ggtgattatg
     4141 tttcaggcac gaaccatata ctgcccacca tgaaggcagc aaggtataca ggtggagtgt
     4201 gggtagggac ctttttgaaa gttgtcagtt tccaaatgat aagtaaagaa ggggcaaaag
     4261 aattgatacc ggcagcaagc agtttagcta aaacagaggg cctttacgca cataagctgg
     4321 cagcagatat aaggctttgg gattaatttc agtattgcaa tgataggaac aaaattggag
     4381 aagggcttct atgaaacctt ctccaattgt atttttttta tgcgctctca taatcgacta
     4441 ggtatatatc atccttagca atctcttttt tgccgttgct caaaacgtta tactttccac
     4501 accttctccc attcccaggc ggaaaagttg caaaaaaaat gaaaggagat ttttgaattt
     4561 ttaaagaaat ataacaccga ttaatctcag cttttctaaa acataaagtt atatggatta
     4621 acaagtcatt ttgctatgga ttatttcttt gcaaaatggc agcttatgtt ttatccttta
     4681 cttgtttaga aaaggccttc tacacttaaa tagcgttcac cggtatcggg aagcacggcg
     4741 actactagct tgcccggacc taaatctttt gccagctttt gggcggcgta aactgcagct
     4801 cctgatgaaa taccggcaag aattccttct ttggttgcaa gcagtcttgc aaaattgtac
     4861 gcttcttcat tgggaacggt aatgatttgg tcaattaaat cagtatctag aacctttggg
     4921 ataaagccgg cgccgattcc ctggattttg tggggtgagg gttttccgcc tgaaagtact
     4981 ggtgatgctt ccggttcaac tgcgacgaat tttacatggg gaagttttgc ctttatggtc
     5041 cttgccactc ccgtaaaggt gcctccggtc cctatgccgc ttacgaatgc atccaatttg
     5101 ccgccggttt gctttagtat ttcctgggcg gtagtccttt catgtatggc gggatttgca
     5161 ggattgtcaa attgtagagg cataaaatag ccctgagttt tagcaagctc ctccgctttt
     5221 tctactgcac ctttcatccc tttttcagct ggtgtcagta caaactccgc gccgtaggcc
     5281 tttaaaagct ttctacgctc gacgctcatg gattcaggca ttaccaatat cagtcgatat
     5341 cccttgactg cgcaaaccat agccaggcct attcccgtat tgccgcttgt aggttcaata
     5401 accgtagagc cgggtttaag gatccctcta tcttcggcgt cttgtatcat gctaaaggct
     5461 atgcggtcct ttacgctacc gccgggatta aatgattcaa gctttgcgac a
//
LOCUS       DAONUO010000120         5504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_10880_length_5504_cov_3.509268, whole genome shotgun sequence.
ACCESSION   DAONUO010000120 DAONUO010000000
VERSION     DAONUO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10880_length_5504_cov_3.509268"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK768_05530"
     CDS             <1..544
                     /locus_tag="PK768_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQA60464.1"
                     /translation="TVSCDLNYRKKLWSREKARSVMTELVQYVDIVIANEEDAADVFG
                     IRAAESDVTKGELSEEGYKDVAKQLMDKFSLKGVAITLRESYSASDNGWSAMFYDGND
                     YYFSRKYKVHIVDRVGGGDSFGGGLIYAMLNGYAPQDAVEFAVAASCLKHTIVGDFNH
                     MTLKEVQNLAKGDASGRVQR"
     gene            894..1898
                     /locus_tag="PK768_05535"
     CDS             894..1898
                     /locus_tag="PK768_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQA60465.1"
                     /translation="MVTIKDVAKKAGVSPSTVSRALSGNGPVKESTRKKVLDAVKLLD
                     YRPNFLAQGLKEGKTKTIGLIIPNIRNPIYPAVARGVEDAAKKFGYTVILCNTDEDVD
                     TEKEYIQKLQKRWVDGIIIAPASNENEHIVKLQNENFPIVVIVRNVDFLSNAVIIDNY
                     RAAYDAVSYLIRTGHRRIAIVKGNQQLALYRERFRGYKQALLDAGIPVVEELIAGDDS
                     DSVQWTLDGYNAINCLFSQNIEFDAVFATTDLRAIGAIRAIKDHGYKVPEDISVIGFD
                     NLEFSSLIDPPLSTVSQPLYDIGMRAVNKLIPLIDNKTVTEPTIEIMLSELIIRKSTK
                     "
     gene            2024..3223
                     /locus_tag="PK768_05540"
     CDS             2024..3223
                     /locus_tag="PK768_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA60466.1"
                     /translation="MKRFLAILLAVFMLFSVAACGSKAPEAEAPAETPQQQEEQPAPE
                     GDIVIGVLQDITGVTSTLGKMVQEGAQWAADEINAAGGVNGRKIKLVTYDTKADVQEA
                     INAFNRMATTDKVSAVIGPPVANIAIAIAPISEQYDVPVLHFAIDDRASIKDDGTPYK
                     NMFLFQPSAKQQGAIMAKFAVVEQGLKKFGIIYNQSNAYSVSLLEPFKTTAESLGAEI
                     VEEVPYQPNDKDFKTMLNKLISAGAEAIYAPNYTQELILIVQQARAIGFEGRLICGLD
                     ACPPFPELCGPESNGVIYINNITETEPQIQGIIEAYKDKTGNDATNKFFLGYDVMNII
                     AKTIGEVGDDPVAIRDAVEKLSGFEGLTGNITIDPNTHQTKGLEMVMHEIQNQAPVML
                     KRYSAEQ"
     gene            3296..4168
                     /locus_tag="PK768_05545"
     CDS             3296..4168
                     /locus_tag="PK768_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQA60467.1"
                     /translation="MSVQIIINGLAVGSVYALIATGFSLIFNILKFSNFSHGALMTLS
                     AFIGYFFAVRTGSSLIPVIIVSMLAGGLVAYLGELVAFRKITLRSTSPFYYFVSSITL
                     GTLYEGIVTIWVGPNFYNYPRFFESMVVKIGDFVISKADLIMFFSSALALALLVIFIQ
                     KTRLGRALRSVSFDRSTAGLMGINVFGIIQLAFFISGALAGLSGVFLGINYTLYPQLG
                     NLVVKGFIASVIGGLGSIFGAVVGAILLGLTETLLINFVGSGYAPVFSFVIMLAFLLI
                     RPQGIAGSNVQEKA"
     gene            4182..5051
                     /locus_tag="PK768_05550"
     CDS             4182..5051
                     /locus_tag="PK768_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQA60468.1"
                     /translation="MQFYISILTFTLISVIAVTGIYVLTGLTGMFSLGQAAFMAIGAY
                     VSGYLVVKLGVPFVLAAIIAVIISVMVGYIVGLPTVRLRRDYISLVTLGFGEAITALL
                     NQAQSITGGALGFSGIPKITTLPLALISAIACIVLISFLKNSKYGRQCIAARSDELAA
                     KTMGINVPQIKMIAFLISVAVTSYAGVLYAFFTTYVDPGIFGWKKSAEWVIMVFFGGV
                     NSLTGSIIGTVILTALPEVLRFAADYRTVFYAVLVLIIINFKPTGLFGEYELSLVNLL
                     KRLSGKKEERANA"
     gene            5044..>5504
                     /locus_tag="PK768_05555"
     CDS             5044..>5504
                     /locus_tag="PK768_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA60469.1"
                     /translation="MLEVKNVSKSFGGVKAIQDVSLTANKGDIIGIIGPNGAGKTTLF
                     NVITGVYKADNGNVILDGKEITKMEQYMITREGIGRTFQNIRLFKGLTVLENVMCAFD
                     PLSKYSIFDGLIPTPRRLAEEKRGREICEHYLNVVGMLQHKNERPENLPYGL"
BASE COUNT         1653 a         1102 c         1248 g         1501 t
ORIGIN      
        1 aacagtgagc tgtgacttaa attatcgcaa aaaactctgg tcaagggaaa aagcccgttc
       61 agttatgacc gaactcgtgc aatatgtgga cattgtcatt gctaatgaag aggatgctgc
      121 cgacgtcttc ggcattaggg ctgcagagtc agacgttacc aagggcgaat taagtgagga
      181 aggctacaaa gacgttgcaa aacagctcat ggataagttc tcactaaagg gcgtggccat
      241 aactcttcgt gaaagttatt cggcatcgga caatggctgg tcagccatgt tctatgatgg
      301 aaacgactat tacttctcca ggaagtataa ggtgcacata gtagacaggg ttggcggtgg
      361 cgactccttc ggcggtggtt taatatacgc aatgctaaat ggctatgcac cccaggatgc
      421 agtagaattt gccgtagctg cctcttgcct caaacatact atagtcggag actttaacca
      481 tatgaccctg aaggaagtcc aaaaccttgc caagggtgac gcttcaggta gggtgcaaag
      541 gtaagacagc aggagtttgc taaatgcaag gaaaaaacat accttcgaat caaagcgaag
      601 gtatgttttt catatttata gaaacttttt atagaaactt ttttcacaag gtagaaggac
      661 ttttaattta tatgtcgaat ttaactatat agaaacgttt ttattaagtt atgtgtaaga
      721 cctgaaaacc ttaaagccct tgatgcataa aggatacaat gtttcaggct ctcattcaaa
      781 acactaaata cgaataaaat gaagcaaaaa cataaaaacg tttgcattta cctgaaacct
      841 gagaattttg ccgatattgt tttggctttc tacagtgtag agaggaagat tcaatggtaa
      901 ccataaaaga tgtagcaaaa aaagccggag tctctccgag taccgtttcc agggcgttaa
      961 gcggaaatgg gcctgtaaaa gaatctacga gaaagaaagt attagatgcc gttaaattat
     1021 tggattatag acctaatttt cttgctcaag ggctgaaaga aggcaaaacc aagacgatag
     1081 gtctgatcat tcctaacata cgaaatccca tttatcccgc tgtagcaaga ggggtagagg
     1141 atgctgccaa aaaattcggt tataccgtaa tcttgtgcaa taccgatgaa gatgtagata
     1201 ctgaaaaaga atacatacaa aagcttcaaa aaagatgggt agatggaata ataattgccc
     1261 cggcatccaa tgaaaatgag catattgtca aattgcagaa cgagaatttt cctatagtgg
     1321 ttatcgtaag aaacgtagat tttctgtcaa atgcagttat aatcgacaat tatagggcag
     1381 cttatgatgc ggtatcctac ctaataagga cgggccaccg gaggattgcg attgtcaaag
     1441 gtaatcagca gctagcccta tacagagagc gattcagggg atataagcaa gctttgctag
     1501 atgctggcat tcccgtagtg gaagagctta ttgccggtga cgattccgat agtgtgcagt
     1561 ggacccttga tggttataat gccatcaact gtttattttc ccaaaatata gagtttgatg
     1621 ctgtttttgc aactaccgac cttagggcca ttggagccat aagagccata aaggaccacg
     1681 gttataaagt gcctgaagat atatcagtaa taggatttga taacctggag tttagctctc
     1741 taatcgatcc gccgctgtct accgtatcac aaccgctgta tgacataggt atgagagctg
     1801 taaataaact aataccctta attgataaca agactgtaac tgaaccaaca attgaaataa
     1861 tgttatcaga gcttataatt cgcaagtcaa caaagtaggg gatttttcta caagaagttg
     1921 caaacgtttg caatttcaag gtaaaaatca gaaacatcct attggtatta atcactacaa
     1981 atagtgatta ataaataaaa aaaattacaa gggggaaaaa gtagtgaaaa ggtttttagc
     2041 cattctgtta gctgtgttta tgcttttcag tgttgctgct tgcgggtcta aggcacctga
     2101 agcagaagct cctgcagaaa ctccgcagca acaagaagag cagcctgcac cggagggaga
     2161 tatcgtcatt ggtgttctac aagatatcac aggagtaaca tcaacacttg gcaaaatggt
     2221 gcaagaaggt gcccagtggg cagcagatga aatcaatgct gccggcggcg taaatgggcg
     2281 caaaataaaa ctggttactt atgatactaa agcagatgtt caggaagcaa tcaacgcatt
     2341 taaccgcatg gccacaacag acaaagtgtc agctgttatt ggacctcctg ttgcaaacat
     2401 cgccatagca atagcaccta tttctgagca gtacgatgtg cccgttctcc actttgccat
     2461 cgatgacagg gcctcaataa aagatgatgg cacaccgtac aagaacatgt tcctattcca
     2521 gccatctgcc aaacagcagg gtgctataat ggccaaattt gctgtagtgg aacaaggatt
     2581 gaaaaaattt ggcatcattt acaaccagtc gaatgcatat tccgtatcgc tgctcgaacc
     2641 attcaaaacc acagctgaaa gccttggtgc cgaaattgtc gaagaggttc catatcagcc
     2701 caatgataag gacttcaaga caatgctaaa caaattaatc tctgctggtg ctgaggcaat
     2761 ttacgcacca aactacacac aagagctcat actgattgtt cagcaggcaa gagcaatcgg
     2821 atttgaagga agactcattt gcggtttgga cgcttgtcct ccattcccag aactttgcgg
     2881 acctgaatca aatggagtta tctacatcaa caacattacc gaaacagagc cacagattca
     2941 gggtataatt gaagcttaca aagataaaac aggcaatgac gcaacaaata aattcttctt
     3001 gggctacgac gtcatgaaca tcattgcaaa gacaatcgga gaagtcggcg atgatccggt
     3061 agccataaga gacgctgtgg aaaaactttc aggctttgaa ggcctaacag gtaatataac
     3121 catagatcca aacacccatc agactaaggg cctcgaaatg gttatgcatg aaatccaaaa
     3181 tcaagcacct gtaatgctaa agcgttactc agcagaacaa tagagaagca ttaagcagaa
     3241 ttatcaatac cccccctgcc aggcaggggg gtattgatag tataggaggt aaataatgtc
     3301 agtacaaatc ataattaacg gattagctgt gggctcagtt tatgctttaa ttgccacggg
     3361 cttctcgctg atttttaata tcctgaagtt ctccaatttt tctcacgggg cgcttatgac
     3421 tcttagcgcc ttcatcggtt acttttttgc agtaagaacc ggcagttcct taattcccgt
     3481 aattatcgtt tcaatgcttg cagggggatt ggttgcctac cttggcgaac tggtggcctt
     3541 tagaaaaatc acccttcgca gcacatcacc tttttattat tttgtgtcgt caatcacatt
     3601 ggggaccctg tacgaaggaa tcgtgacaat ttgggttgga cccaatttct ataattatcc
     3661 gcgctttttt gaaagtatgg tagttaaaat tggggatttt gtcatatcaa aggcggattt
     3721 gatcatgttt ttctcatccg cattggcatt agccctctta gtaatattta ttcagaaaac
     3781 acgactgggg cgtgctcttc gctcagtatc ttttgataga agcactgcag gtcttatggg
     3841 tataaatgtt tttggaatta ttcagcttgc cttcttcata tcgggggcac tggcaggtct
     3901 ttcaggagtt tttttaggca ttaactatac tctgtaccct cagcttggca atttggttgt
     3961 aaaaggattt atcgcttccg taataggagg cttgggcagt attttcggtg ctgttgtggg
     4021 tgcaattttg ttgggcttaa cggaaacgct gctcattaat ttcgtaggtt ctggttatgc
     4081 accggttttt tcgtttgtaa tcatgttggc atttttgctg ataaggccgc agggtatcgc
     4141 aggctccaac gttcaggaga aggcttaagg gaggtaaata catgcagttt tatatatcta
     4201 tactgacatt tacacttatt tcagttatag ctgtaacagg tatatatgtc cttacaggtc
     4261 tgacagggat gttttcacta ggtcaagcag catttatggc tatcggtgca tatgtttctg
     4321 gctatcttgt ggtcaagctt ggagtcccat ttgtgctggc agctataata gctgtaataa
     4381 tcagcgtaat ggtgggttac atcgtgggct tgcctaccgt gcgattgcgc cgagactata
     4441 tttccttggt tacacttgga tttggtgagg ccataacggc actgctgaac caagcccaat
     4501 ctattacagg aggtgccttg gggttctcag gtattccgaa aatcaccaca ctcccactgg
     4561 cactgatatc tgcaattgcc tgcattgtcc taataagttt tttaaaaaac tccaagtacg
     4621 gcagacaatg cattgcggca cgcagtgatg aacttgcggc caaaacaatg gggataaacg
     4681 ttccgcaaat caagatgata gccttcttaa tctccgtagc ggttacatct tatgccggtg
     4741 tgctctatgc ctttttcact acctacgttg accctggcat cttcggctgg aagaagagcg
     4801 ccgagtgggt catcatggta tttttcggag gagttaacag cttgaccggt tctatcatag
     4861 gtacggttat cctaaccgcc ctgcctgaag tactgagatt tgcggcagat taccgcacgg
     4921 tgttttatgc agtattagtg ctaatcatca taaactttaa acccacaggg ctgttcggcg
     4981 aatacgaact ttcacttgtg aatttgctca agaggctaag cgggaaaaag gaggagaggg
     5041 ccaatgcttg aagtcaagaa tgtaagcaag agtttcggcg gagtcaaagc gatacaggat
     5101 gtctctctca ctgctaataa aggtgatatc ataggcatca tcggccccaa cggtgccgga
     5161 aagacaacat tgtttaacgt aataaccgga gtatacaagg ctgataatgg caatgtcatt
     5221 cttgacggca aagagattac aaaaatggaa cagtacatga tcacccggga aggaattggc
     5281 cgcacctttc aaaatattcg tctctttaaa ggcttgacgg tcttggaaaa tgtcatgtgc
     5341 gcttttgacc ccttgtccaa atattctatt ttcgacgggc taattccaac acctcgccgc
     5401 ttagctgaag aaaagcgtgg cagagaaata tgcgagcatt accttaatgt agtcggaatg
     5461 ctccagcata aaaatgaacg gccggaaaac ctcccgtacg gtct
//
LOCUS       DAONUO010000121         5436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11049_length_5436_cov_2.955399, whole genome shotgun sequence.
ACCESSION   DAONUO010000121 DAONUO010000000
VERSION     DAONUO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11049_length_5436_cov_2.955399"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..2360)
                     /locus_tag="PK768_05560"
     CDS             complement(72..2360)
                     /locus_tag="PK768_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQA60470.1"
                     /translation="MQRHTITIYYKNSIDTVDLGTFNKNTVTMGRDPSNDIVIDSPII
                     SSHHALFQFVDDSWVLYDNNSKNGIMVNGKKEKMQRLNNNDILQIDNEKRPLKAAVVI
                     IYTVGAENAGEKWWEYAVHSNDFIYIGRDEQNSIVLNHNTISKKHAVFFHSDGKYYIE
                     DLKSTNGTYLNGTRITSMQPVKMNDVIYIGDTKIIFNGKSLLYNYYSKGFGIDAVSLR
                     RTVTVRNSSVFRKKKKTILSDVSVSINPGELVAIIGTSGAGKTTLLKCLSGLTKPDCG
                     TVMVNGDDFFKNYDIYMNILGYVPQEDAVYDNLTVEELLYYSINLRKSALSKTEIMNQ
                     IRQTIKDVELTGMEHTLIKNLSGGQKKRISIAMELLTEPKLLFLDEPTTGLDPQTEFN
                     IAMLLKKLSHDQKTVIFTTHSTSYLDVCDKIIVMGQEGRLCYFGTPKGTLDFFEVKDY
                     AHIYNLIHKNIEYWEDKYKKAHIRHKPLENNKASRHPRHFASIGTMSFQTIVLILRYL
                     KLNLRDKMRLAFLFMQVPLIAFLLGLITEGSKAFKIREEASQIILIVACAAVWVGFLN
                     SVQEISKERGIYNRERNAGLSSIAYIFSKIFVLGLFSLIQSFIFVEVLSWVIELPKPS
                     LLGSVKMELYATIFLTTLVSSVLGLCISSLVKNNDRALGIIPVVLIYQILFSGILFES
                     RKFIQKLSWTAVSHWAVGALGISVNLNDIPFSSPLVLKRNDISYVYSTENLLTHWAVL
                     TLIGVIFTVLTVLALNLNRKRE"
     gene            complement(2537..2854)
                     /locus_tag="PK768_05565"
     CDS             complement(2537..2854)
                     /locus_tag="PK768_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60471.1"
                     /translation="MTDFSDCFNYCTMLAEIPEGLFANNTKVTSFMGCFNNCVNIKEI
                     PEGFFANNTEVRDYSKCFYGCSQLTGSAPNLWFKLNVIEYEDCFYGCTKLSNYEEIPD
                     DWK"
     gene            complement(2893..3438)
                     /locus_tag="PK768_05570"
     CDS             complement(2893..3438)
                     /locus_tag="PK768_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQA60472.1"
                     /translation="MFAAADCYCSCLQLCFCRATLLYGDLNSDGLIDSTDLTMMKRVI
                     LRKLTADDITAADLDGDGELNSTDITLLKRYILKKITKFPVEELNPAITPTPTQEPTP
                     TPTETNEEVFAFKIKIDDKDLTYSFPIESSQTEYNIVVDWGDGIISEITDYSAREHEY
                     GEEGIYTIKVLSFDNMPHVPA"
     gene            complement(3752..4453)
                     /locus_tag="PK768_05575"
     CDS             complement(3752..4453)
                     /locus_tag="PK768_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60473.1"
                     /translation="MKGLKRIVIVTMLLALCTMALEGCRNEADSPGNELTGTPINSSK
                     GMQTGISAGTPGDSSQDASEEVIKEGKIEKKGNVILKQLAFVYQGNTIALSDIVDDKK
                     IESMLGKADEIKSHTYSVGDGKNMDPLNGLTGKQYYYPGLVIKTIDATEDKKSYIFSI
                     EITDPKYPTIRNIKVGDSFEKLKEVYPEGILLGGELTDEEDDYRYEPVNYVDVMNFHI
                     KEKKIESILIYTLLD"
     gene            complement(4556..>5436)
                     /locus_tag="PK768_05580"
     CDS             complement(4556..>5436)
                     /locus_tag="PK768_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQA60474.1"
                     /translation="FLSAGGFTVLIALSSLYIARKTTKPIKHLLSATEKFSRQEFEQV
                     DLNRKDELGMLAEGLNQMGVKLNGYIQYQKQFVSNVSHELKTPLAAIRGFSQYLYEDK
                     NDDADLKKISFHLLNESNRLTKLIDELLILSKFDKGTQELNTEKEDLSELTDSVIQEM
                     KPEADKREISIESALQKGVFANVNKILMSHAIANILDNAIKYSKEGGHITVETSADQN
                     TAIIKITDQGIGINKEELPLVQERFYRSANSNIVKGSGLGLSLCREIVEKFNGRLIIE
                     SEIGTGTTVSLLLPIA"
BASE COUNT         1589 a         1128 c          909 g         1810 t
ORIGIN      
        1 catacaaata ttatatcaaa tccctgaata atagctatat aatttttaca taaaatatat
       61 ttttactcat ttcattccct ttttcgattc aagtttagag ctaaaacggt taatacggtg
      121 aaaatcaccc cgatcaatgt caataccgcc caatgggtca atagattctc tgtactgtat
      181 acatatgaaa tatcgtttct ttttaacaca agaggagaac tgaagggtat gtcattcaaa
      241 ttaaccgata ttcccaatgc tcctacagcc cagtgactta cagcagtcca tgaaagtttt
      301 tgaatgaatt ttcgggattc aaacaagatg cctgaaaaaa gaatctgata aatcagcact
      361 accggtataa tgcctagggc acggtcatta tttttcacca atgatgaaat gcacagtccc
      421 aaaacagatg acaccaaagt cgtcagaaat atcgtcgcat acaattccat cttaacggat
      481 cctagaagtg aaggttttgg gagctctata acccaagata aaacttcaac aaaaataaag
      541 ctttgtatca atgaaaaaag acccagtaca aatattttcg aaaaaatata ggctatgctg
      601 ctaaggccgg cattcctctc tctattataa ataccccgtt ccttggatat ttcctgaacg
      661 gaatttaaaa atccaaccca tacagcagca caagcgacaa tcaatattat ttgactggcc
      721 tcttcgcgga ttttaaaggc tttgctgcct tcggttatca ggcctaaaag aaatgcaatt
      781 agcgggacct gcataaaaag aaaagccaat cgcattttgt ccctgaggtt aagcttcaaa
      841 taccttaaaa tcagcacaat cgtctgaaat gacattgttc cgatgcttgc aaaatgccgt
      901 ggatggcgtg aagccttatt attctcaagg ggtttgtgac gaatatgagc ttttttatac
      961 ttgtcctccc aatactcaat gtttttgtgt atcaggttgt aaatatgggc ataatccttt
     1021 acttcaaaaa aatccagcgt tcctttaggt gtcccgaaat agcacagtct gccctcctgc
     1081 cccataacta tgattttatc gcacacatca agatatgaag tggagtgcgt tgtaaaaata
     1141 accgtttttt ggtcatggga caatttcttt agaagcatgg caatattaaa ttcggtttga
     1201 gggtcaagcc ctgttgtggg ttcatccaaa aaaagcaatt tgggttcggt caaaagttcc
     1261 atcgctatac tgatgcgttt tttctgcccg ccgctcaagt tctttattaa agtatgctcc
     1321 atccctgtca gctcaacatc ttttattgtc tgtcgaattt gattcattat ttcagttttg
     1381 ctcagtgccg attttcgaag atttatcgaa taataaagca gctcttcaac ggtcagatta
     1441 tcatataccg catcttcctg aggcacatat cccaagatat tcatgtaaat atcatagttt
     1501 ttaaaaaaat catcgccgtt taccataaca gtaccgcaat cgggtttggt taatccgcta
     1561 aggcatttga gaagcgtagt ctttcccgca ccgctggtac ctattatggc aacaagctct
     1621 ccgggattta tgcttacgct gacatcactg agtattgtct ttttcttttt tcgaaagaca
     1681 gaactgttcc taacggttac agtccttctt aaagaaacgg catctattcc gaatcccttc
     1741 gaataataat tatatagcaa ggatttaccg ttaaaaataa tcttcgtatc cccaatgtaa
     1801 atcacatcat tcattttcac cggttgcatt gatgtgattc ttgtaccgtt taaataagta
     1861 ccgtttgtgc ttttcaagtc ctcgatataa tattttccgt cggaatgaaa aaatacggca
     1921 tgttttttgg atatagtatt atgattcaat acaatactgt tttgttcatc tctaccaata
     1981 tatatgaagt cgttagaatg gacagcatac tcccaccatt tctcaccggc attctcagct
     2041 cccacagtgt atatgatgac aacagcagct ttcaacggtc ttttttcatt gtcaatctgc
     2101 aggatatcgt tattattcaa gcgttgcatt ttttcctttt tgccgttaac cattattccg
     2161 tttttgctgt tattatcgta taatacccaa gagtcatcca caaattgaaa taatgcatga
     2221 tgagaagaga ttatgggcga atcaatgaca atatcgtttg atggatctct ccccatcgtt
     2281 acggtatttt tattaaaagt gccaaggtca actgtatcaa tagaattctt ataatatatt
     2341 gtaattgtgt gcctctgcat atcgttatgt acccttcgca tgtttacata ttttattcca
     2401 atgatattat aaatcagcaa aaacgattaa tcaaatatat tagtgttgta taatattttg
     2461 aatcttatat ctcattgcat atctattcat ttgcattggc aacaggcttg caccgacaca
     2521 taaaacaggg aaaaattcac ttccaatcgt ccggtatctc ttcgtagttg gataatttag
     2581 tacagccata aaaacaatct tcatattcta ttacatttaa tttgaaccat aaattcggtg
     2641 ctgaaccggt taattggctg cagccgtaaa aacacttgct gtaatccctt acttcagtat
     2701 tattagcaaa aaatccttca gggatctctt ttatgttgac gcagttattg aaacagccca
     2761 taaagctggt tactttagta ttatttgcaa aaagtccttc cggaatctct gctaacatgg
     2821 tgcaataatt aaaacagtca ctgaagtcag tcacattagt attattggca aacagccctt
     2881 cgggaatttc tgttaagccg gtacatgcgg catattatca aaggaaagta cttttattgt
     2941 atatatacct tcttctccat actcatgttc tcttgcagaa taatctgtta tctcgcttat
     3001 tatgccatcg ccccagtcga caacaatatt atattctgtt tgtgaagatt cgatgggaaa
     3061 gctatatgtg agatctttat catcaatttt tatcttgaat gcaaaaactt cttcatttgt
     3121 ttctgtagga gttggtgtag gttcctgcgt aggggtgggt gttattgccg ggttcaattc
     3181 ttccaccgga aactttgtta ttttcttaag tatgtacctc tttagcagtg ttatatccgt
     3241 tgaattcaat tcaccgtcac cgtcaagatc agcagctgtt atgtcgtcgg cagtcaattt
     3301 tctgagtatt accctcttca tcatagttaa atcagttgag tctattaagc catctgaatt
     3361 taagtcacca tataataaag tagcccggca gaaacaaagc tgaagacaac tgcagtaaca
     3421 atcagcagca gcaaaaatgt tttttcatca taaaactccc tcccctttat attaaatact
     3481 taattaatgc acataaagaa caacacaaga gatttccaat tcattctatc gctagtgatt
     3541 aacaacagta ttaacaattt aattatattt tacttgaaaa ttattgtaaa gtttattatc
     3601 tcattttgcc gcaatgaatt attaaaccgt aaatgggaaa aatcggcata agatcaaggg
     3661 gacggttctt ccgtgtcgta tgacacggaa gaaccgtccc ctcgtgcggg agaaccgtcc
     3721 ccttgtgcta ctcttgtttg atgtggcggg attaatccaa aagcgtatat attaaaatgc
     3781 tctcgatctt tttctctttt atatgaaaat tcattacgtc aacgtaatta accggttcat
     3841 accgataatc atcctcctcg tcagtcaatt ctcctcccag caggattcct tcaggataaa
     3901 cctctttgag tttttcgaag ctgtcgccaa cttttatatt ccttatagtt gggtatttcg
     3961 ggtcagtaat ctcaatgctg aaaatatacg actttttgtc ttcagtagca tcaatagttt
     4021 ttataacaag ccccggataa taatattgtt ttccagtcaa tccgttaagg ggatccatat
     4081 tctttccatc cccaacagaa taggtatgag acttgatttc atctgctttt cccagcatac
     4141 tctcgatttt tttatcatcc acaatatcgg aaagggcaat tgtattccct tgatagacaa
     4201 aggccagctg ttttaatata acattcccct tcttttctat tttgccctcc tttattactt
     4261 cttctgaggc atcctgggaa ctgtccccag gggtaccggc ggatatgccg gtctgcattc
     4321 cttttgagga atttatcggt gtaccggtta attcattccc cggtgagtca gcttcattcc
     4381 tgcacccttc cagagccatt gtacaaagtg ctaaaagcat tgtcactata acgattctct
     4441 ttaaaccttt cattgccgct cctccttcaa ataatctgaa atgtttatgt tacagtgttc
     4501 attatatata aaaactatgt tagggtaata acaaactggt atcaatcttg taacattatg
     4561 ctatagggag cagaagggaa acggtagttc cggttccaat ctcactttct atgattaacc
     4621 ggccgttgaa tttttcaaca atctctctgc aaagagaaag ccccagcccg gaacctttga
     4681 caatattaga attggctgac ctataaaacc tttcctgcac caaaggaagc tcctccttat
     4741 taataccgat accttgatcc gtaatcttta tgattgctgt attttgatcg gcagaagtct
     4801 ctactgtgat atgacctcct tctttcgagt actttattgc attgtctaaa atatttgcaa
     4861 ttgcatggga catcaatatt ttattaacat tagcaaagac tcctttttgt aaggctgatt
     4921 ctatcgatat ttctctttta tctgcctccg gtttcatttc ctgaataaca ctgtcagtca
     4981 gctccgataa atcttccttt tctgtgttaa gctcctgagt tcccttatcg aattttgaaa
     5041 gaattaaaag ttcatcaata agctttgtaa ggcggtttga ttcatttagc agatgaaagg
     5101 atatcttttt taaatcagca tcgtcatttt tatcttcata taagtattga gaaaacccgc
     5161 gaattgctgc caacggagtt ttcagctcat gagacacatt agagacaaac tgcttttgat
     5221 actgaatata gccgtttagt ttaacaccca tctgatttaa tccttctgcc agcatcccca
     5281 gctcatcttt cctgtttaag tcaacctgtt caaattcctg ccttgagaac ttttccgtcg
     5341 cagaaagaag atgttttatg ggctttgttg ttttacgggc aatgtacagg cttgacaggg
     5401 ctatcaaaac agtaaacccg cctgcactaa gaaata
//
LOCUS       DAONUO010000122         5391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11160_length_5391_cov_3.878373, whole genome shotgun sequence.
ACCESSION   DAONUO010000122 DAONUO010000000
VERSION     DAONUO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11160_length_5391_cov_3.878373"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(563..2152)
                     /locus_tag="PK768_05585"
     CDS             complement(563..2152)
                     /locus_tag="PK768_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA60475.1"
                     /translation="MSVIYNNLTRELKKEVIHHTIFISKAIELNADSYMPYITAVGKE
                     SKSRISLISADGTVLYDSFAPAEKLENHGDRPEIKSAMSSGTGEATRLSDTLGEKTYY
                     YAVKLSNGNILRVAATSKSILGLIRSAVPWIVIIAIAILVLAVIIARVITDLIIKPIN
                     KLNLNDPLSNRTYEELAPLLSRLEKQNQKILAQVKKLTSQQHEFEYITGNMKEGLVFL
                     GENGTILFENNSAKRILGDAVGKSYLELHRDVQYIKAIDSALAGKALRVKMNKYSKMY
                     ELSANPVDGKSNAYAAVLFIVDITAKENVEKLRREFSANVSHELKTPLTSIMGYAEII
                     ENGIAKQEDIPDFANSIYGEASRLLALIEDIIKLSRMDERDLDQEFAEVDLSELCESV
                     INNLSEKAKTKNIKIDYSGNTSIVYGIKPILYEVIYNLCDNAIIYNKDNGSIKITLLV
                     ENNKTKLSVSDTGIGIAPHHHDRIFERFYRVDKSHSKETGGTGLGLAIVKHAAMLHRA
                     EIALKSQEAKGTTITIAFPNN"
     gene            2339..3115
                     /locus_tag="PK768_05590"
     CDS             2339..3115
                     /locus_tag="PK768_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA60476.1"
                     /translation="MENRIKVKKIDDCVWLMDDKGEATGYLVCGEKKAVVIDTMNGIA
                     DVHAVVRAITDLPLTVVNTHGHCDHIFGNLYFDCDCFLHPGDTEVAAEHTGFPGFVKL
                     RRKKKLEMPPFKEINDGEIIDLGGLTLEVISLPGHTPGGICLLLREKRILFTGDSINR
                     HLWMQLKESLPLRTLLENLDKISWVTEKADKILHGHSQDFDDISLFNELHSGLRELIE
                     TKGEGDTRRSTDYKWFGGTAKQYLFGDGRSVICYSSDKLD"
     gene            3342..3893
                     /gene="bfr"
                     /locus_tag="PK768_05595"
     CDS             3342..3893
                     /gene="bfr"
                     /locus_tag="PK768_05595"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HQA60477.1"
                     /translation="MKQTNEQDGSCQDPYQIIGIALKKGDVLMKGSPKLIETLNALLA
                     DELAAINQYIVHAEMCEDWGYTKLHEGFEKRSIEEMKHAEKLIGRILFLGGTPIVTEL
                     SPISIGAEVPKQLEFDRAAEEAAIKAYNDAIALAAEERDHATRDILIQILNDEDRHMD
                     ELEELLDQIEQMTLPIFLTTQVE"
     gene            4012..4257
                     /locus_tag="PK768_05600"
     CDS             4012..4257
                     /locus_tag="PK768_05600"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HQA60478.1"
                     /translation="MKILLSLYLVFAVILAQFFAAYFFSKGRSSYRKAFSAMVLCVSI
                     YLFGYLMIINNNTLQEMIFWKSGPWPGKLFLKTILPA"
     gene            4254..5078
                     /locus_tag="PK768_05605"
     CDS             4254..5078
                     /locus_tag="PK768_05605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQA60479.1"
                     /translation="MIVLGPGKRIIDYNRAAEKFFRAINISLDHYPIDQILANEPGML
                     EVFESEASRGLSLIIDGEERFFEIDTLPLGNPQDGNTKMLKSIRDVTEERRIQEKLKF
                     LATTDSLSGLCNRAEFMNLARKEFALAKKNNEEMSLLIMDLDNFKTINDTLGHAAGDE
                     IIRKMGDIIKKSFRKTDIPGRIGGEEFAVVLKNTSLQEAKKAAEKVRNISTRTKVIYG
                     EHEITFTVSIGVAASGGNTGDIEDIMKMADDALYKAKAKGRNCVVVLEPGPDSDGN"
     gene            5163..>5391
                     /locus_tag="PK768_05610"
     CDS             5163..>5391
                     /locus_tag="PK768_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60480.1"
                     /translation="MFKRAKAIVFILLAACTVSMLSPVSFAAGVTSLQERTPGEIISK
                     YWEKPFQLRNIGPAEYVVEPSSSHPYVAGKVR"
BASE COUNT         1605 a         1020 c         1250 g         1516 t
ORIGIN      
        1 attgatatgt tcccgtaaac gtaaggttca aaaaataagc ccaagacatc aatccaagcg
       61 gctcaagcca tgttgagacc atttgcctga tgtcgaaata tgagctatga attaaataga
      121 aatcgaattt aaaagcggtt ttcagacttt taacaacaat tgttgaggta tgggagccgt
      181 atttttatat atgtagtatg gtatgtaata aggatgaaca ggtctcataa atgctttatg
      241 caaagcaaaa ccttgtttta atttagcaaa ataaaaattt atggtacact tggaaagaaa
      301 ttgatttact aatgttagag aagttaaaat caaaagtaga tcctactaaa gatatactca
      361 tcaaaaatgc agtttaagaa aagatcagac tataagtgag aatcaagcca tggaaggagg
      421 aagtcaatga gtaatcaaaa tatttacgac aaccaggagt tttttgatgg ttataaaaat
      481 taagaagtaa tctttctgtg gcaaatgaaa aatcaaaatg ctatttctat aaccagcgaa
      541 ggttttagtc taatacagga gtctaattat ttgggaaagc aattgttatt gtcgttccct
      601 tggcttcctg acttttaaga gcgatttctg ctctgtgaag catggcggca tgtttaacaa
      661 ttgcgagccc tagtccggtg ccgccggttt ctttcgaatg gcttttatcg acccggtaaa
      721 atcgctcgaa gatacggtca tggtgatgcg gtgcaatgcc tatacccgta tcagaaacag
      781 ataattttgt tttattgttt tcaactagta gcgtaatctt aatgcttccg ttatccttat
      841 tataaataat agcattatcg catagattat aaatcacctc atatagtata ggctttatgc
      901 cgtaaactat agaagtattg cctgaatagt ctattttaat attctttgtt tttgcttttt
      961 cagataagtt gtttataaca gattcacaca gctcagagag atctacttct gcaaattcct
     1021 gatctaaatc tcgctcgtcc attcttgata atttgataat atcctcaatc aaagccagta
     1081 agcggctggc ttcgccataa atagagttgg caaagtccgg aatgtcctct tgtttagcga
     1141 ttccattttc tatgatttcc gcataaccca taatagaggt caagggtgtt ttaagttcat
     1201 gggaaacatt tgccgaaaac tctcggcgca acttttctac attctcctta gcagtaatat
     1261 ccactataaa taaaacagca gcataggcat tggatttgcc atccaccgga ttggcagata
     1321 gctcatacat cttgctgtat ttgttcattt ttactcgcaa ggcttttcct gcgagggctg
     1381 aatcaatggc ttttatatac tgtacatccc gatgcaattc taaatacgat ttccccacag
     1441 cgtcacctaa aatccgcttc gcgctgttgt tttcaaatag aatagtgcca ttttcgccga
     1501 gaaaaacaag cccttccttc atattgccgg ttatatactc aaattcatgt tgctgtgaag
     1561 ttagcttttt tacttgggct aatatctttt ggttttgctt ttcgagtctt gatagaagag
     1621 gcgccagctc ctcatatgtg cggttggaga gggggtcatt caaattaagc ttatttatcg
     1681 gttttattat caggtcggtg atcactctag caatgattac cgctaataca agaattgcaa
     1741 tagcgatgat cactatccaa ggtacagcgc tcctgattag ccctaaaata cttttgctgg
     1801 ttgcagcaac ccttagtata ttaccgttgc taagttttac agcataataa tatgtttttt
     1861 cacctaaagt gtctgataga cgagtagctt ctccagtccc gctggacata gcggatttta
     1921 tttctggtct gtcaccgtga ttttccagtt tttcagctgg tgcaaagcta tcgtatagta
     1981 ccgtaccatc tgcagatatt agggagatgc ggcttttgct ttctttgcca actgcggtga
     2041 tatatggcat atagctatcc gcatttagct caatggcctt actgataaaa atggtatggt
     2101 gtattacttc cttttttaac tccctggtta agttattata aattacactc ataataaatg
     2161 ttaatgttaa tttatagcaa gcgtggggat cagtcctctt ggcgcttttt gctttcagcc
     2221 attctggaac aattaatacc aggaaaccat tgacaagttc tcaagtacag atactataaa
     2281 aatagaatat tcaaaaaacc agactcggca cttgatgtca tggagaaggg gtgtttgcat
     2341 ggaaaacagg ataaaggtca aaaaaatcga cgactgtgta tggcttatgg acgataaagg
     2401 cgaggccacc ggttatctgg tgtgcggcga gaagaaagct gtggtcattg atacaatgaa
     2461 cggtatcgca gatgttcatg cggttgtcag ggcaatcacc gacctgccgc ttacagtggt
     2521 aaatacacac ggccactgtg accatatttt cggtaatttg tactttgatt gcgattgctt
     2581 tctgcatccc ggtgatacgg aggttgctgc tgagcatacc ggttttcccg gatttgtgaa
     2641 gcttcgcaga aaaaagaagc ttgaaatgcc gccgttcaag gagatcaatg acggcgaaat
     2701 tatcgacctc ggcgggctta cacttgaggt aatatcgctg cctgggcata cccccggggg
     2761 aatatgcctg cttttgagag aaaagcgcat actgtttacc ggtgacagca taaatcgtca
     2821 tctttggatg cagctgaagg agagtctgcc gcttcgaaca cttctcgaaa atcttgataa
     2881 gatctcgtgg gtgacagaaa aggctgacaa aatcctgcac ggccattcac aggatttcga
     2941 tgatatttcg ctgtttaatg aattgcactc cggcctgaga gagctcatcg aaacgaaggg
     3001 tgagggtgat acgagacgca gcacagatta caaatggttc ggcggtaccg cgaaacagta
     3061 tctcttcggc gacggcagaa gtgttatctg ctacagcagc gacaagctgg attgagggac
     3121 agtggcaagt tcgattcaga gagcgcaaca agctggattg aaaggacgat attgcggttg
     3181 ataaaaagag cggttactgg taaaatgtca atatacagat attagtatcc atgttataga
     3241 aatatggata ctggaaggtc gctaacagat gttatgcggc tgatgaaaga tagtggacca
     3301 tcaaattttt ctgttttccc ggaccgaatc cagggaataa attgaaacag accaatgaac
     3361 aagatggttc atgtcaggat ccataccaga ttatcggtat tgctctaaag aaaggagatg
     3421 ttcttatgaa agggtcccca aaacttattg aaacgcttaa tgctttgctg gcagatgaac
     3481 ttgcggccat caatcagtac atcgtacatg cagaaatgtg cgaggactgg ggctacacca
     3541 aattgcacga aggctttgag aaacggtcaa ttgaggaaat gaagcatgct gaaaagctca
     3601 tcgggaggat tctgttcctc ggtggaacac ctatcgtaac tgaactaagc cctatcagta
     3661 tcggtgctga ggttccaaag cagttggaat ttgaccgtgc tgcagaggaa gccgcaatca
     3721 aggcttataa tgatgccata gcactcgctg cagaagagcg tgaccatgcg acaagggata
     3781 ttcttataca gatcctgaat gatgaagacc gtcacatgga cgagcttgag gagcttctgg
     3841 atcagattga gcagatgact ctgcccatat tcctgacaac gcaggttgag tagtatcaag
     3901 agatgcagtt tgcgattcag ggctgttgat aaagcagccc cttttgcgtt ccgtgatcat
     3961 aattatttct tgtggctcaa tgccagctga tgaatcaata aaggtattgc catgaaaata
     4021 ttattaagtc tttacttggt ttttgcagta atattggcac agttttttgc cgcatatttc
     4081 ttttccaagg gcagaagcag ctatcgaaag gcattctctg ccatggtttt gtgtgtcagc
     4141 atttatctgt ttggttattt gatgatcatc aacaataaca ccctgcagga aatgattttc
     4201 tggaaatcag gaccctggcc cgggaaacta tttttgaaaa caattttgcc ggcatgatag
     4261 tactggggcc cgggaaacgg ataatagatt acaacagggc agcggagaag ttttttagag
     4321 cgataaacat ttcattggat cattacccta tagaccagat cctggccaat gaaccgggga
     4381 tgctggaggt ttttgaaagt gaggccagtc gcggtctctc tttgataata gatggcgagg
     4441 aacgcttttt tgagatcgac acgttgccgc tggggaatcc gcaggacgga aacacaaaga
     4501 tgttgaaatc catccgtgat gtaacagaag aaaggaggat ccaggaaaaa ctgaagtttc
     4561 tggccactac agattccctg agcgggcttt gcaatcgggc agaatttatg aacctggccc
     4621 gaaaggaatt tgcattggct aaaaagaata atgaagagat gtcgctgctg ataatggatc
     4681 tggataattt caagaccatc aacgataccc tgggacatgc agcaggagat gagatcattc
     4741 ggaagatggg agacatcata aagaaaagtt tccgcaaaac cgatatcccc ggacgtatag
     4801 gaggagagga attcgctgtg gttttgaaga atacatcttt gcaggaagca aaaaaagcag
     4861 cggaaaaggt ccggaatata tcgaccagga caaaggtgat ttatggggag catgaaataa
     4921 cttttactgt aagtatagga gtggcagcgt caggtggaaa taccggtgat atcgaagata
     4981 ttatgaaaat ggcggatgat gctctgtaca aggcaaaagc aaaagggcga aattgcgtcg
     5041 tagtcctgga acccggaccg gacagtgacg gaaattgata tgctgtgata agcagtggga
     5101 atggttacaa cgggatcaaa tgatctgtca taacggagaa atccagtagt gggaggtttg
     5161 aaatgttcaa aagggcaaaa gctatcgtat tcattcttct ggcagcatgt acagtaagca
     5221 tgctgtcacc ggtaagtttt gcagccggtg tcaccagcct tcaggaaagg accccgggag
     5281 agataataag caaatactgg gaaaagcctt ttcaattaag gaatatagga ccggcggaat
     5341 atgtcgtcga gccaagctcc agtcacccct atgtcgctgg caaagtcaga g
//
LOCUS       DAONUO010000123         5366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11221_length_5366_cov_3.676520, whole genome shotgun sequence.
ACCESSION   DAONUO010000123 DAONUO010000000
VERSION     DAONUO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11221_length_5366_cov_3.676520"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PK768_05615"
     CDS             complement(<1..965)
                     /locus_tag="PK768_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HQA60481.1"
                     /translation="MEIKIIKQEEKELFNEFMAWGPKGHILQSYEWGEVKRRTGWEPI
                     RLLVMDGGKPVAGISILKRRLPIPGFNKCIFYAPRGPVADYSDEKTLKFLFTQVKAIA
                     KKHGSVFLKIDPDIKAPDERVVKNLRSVGFTHVDSGKDFDGIQPKFVFRLDINNKSLD
                     EIMAGFHHKTRYNIRLAARRGVTVKVGEREDLKPFYDILEETCIRDKFLIRGFDYFET
                     LWDELVEKGLARLFMAEYEGKYIAGTLAFIFGDKAWYIYGASSNSHRNVMPNYALQWE
                     MIKWAKENNCTMYDFRGVSGDLSSDNPLYGLYRFKKGFNGEFTEF"
     gene            complement(1045..2304)
                     /locus_tag="PK768_05620"
     CDS             complement(1045..2304)
                     /locus_tag="PK768_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HQA60482.1"
                     /translation="MKNIRARVAIVTMILVAASLGGYTYYFHGEDFANYMNLLAIGIA
                     ALISGTVSFLITRETALPVDKIVQSFEAMSKGDFTVKIKKTAGGEIGKLADLYNNSLE
                     RIKSILKSAKDITDEILSSADLMDSMAKQSHQASSSYEGSMKESIEKSSESLSEMQKL
                     VDNISDMLSQMSAGVEQIAASSSEAAINAADTSEMANSGKTLMDKVAEQMEKIQESNR
                     YTSTVIKSLGESSETIGQIVETITGIADQTNLLALNAAIEAARAGEQGRGFAVVADEI
                     RKLAEQSGEAAKQIGELITSVIHEVEKAVKAKDEGSANVQQGINAVKDASAAFEKILD
                     AVTNVAHQMQEISAATQQMSASAENTSQYFQQMTDISIDASNRVHEIAEHVEIQHVLL
                     GEIAISVEEIKEDVGRLAQVMEGIKIQ"
     gene            complement(2486..4090)
                     /locus_tag="PK768_05625"
     CDS             complement(2486..4090)
                     /locus_tag="PK768_05625"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQA60483.1"
                     /translation="MSTKYIFVTGGVVSSLGKGITAASLGRLLKSRGLSVTMQKCDPY
                     INVDPGTMSPYQHGEVFVTDDGGEADLDLGHYERFVDINLTRNSDTTTGAVYLSVITR
                     ERQGGYDGKTVQVIPHITNEIKERIRKVGEEFKADIVITEIGGTVGDIESLPFLEAIR
                     QMKTEVGRDNVLYIHVTLVPYISTAGELKTKPTQHSVKELRSIGIQPDIIVCRSERPL
                     SQDVKEKIALFCNVEPECVIQNYDAETIYEVPLILKNEGLDQIVAKKLGVDGMKTDLS
                     EWEEIVRKIKNPTKELNIALVGKYVELHDAYLSVAEALCHGGIANDVKVNIKWVHSEK
                     LEENDVDLASTFNNIDGMIVPGGFGQRGIEGMIRAIKFARENKIPFFGIGIGMQCAVV
                     EFAQNVCGLKDAHTTEVMPDTPNPVIDFPKTNCIDDKTMRLGSFACKLSENTIARVAY
                     GTEIIMERHRHRYEFNNAYRDSLSKCGMVLSGISPEENFVEIIELKDHPWFVATVFHP
                     EFKSRPNNPHPLFTHFIKAALHAAGK"
     gene            complement(4184..4408)
                     /locus_tag="PK768_05630"
     CDS             complement(4184..4408)
                     /locus_tag="PK768_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60484.1"
                     /translation="MALLIISVVIILMLELPGLIKSRVWRELAVFCGLLIIGIAYGII
                     HIFKLGILNPTKLTQYVIEPIFKAVDNMLK"
     gene            complement(4435..>5366)
                     /locus_tag="PK768_05635"
     CDS             complement(4435..>5366)
                     /locus_tag="PK768_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HQA60485.1"
                     /translation="LPRREYIAHSNIVIIGEDLAREGVDQLIDVLLRSKDIRLNTWVL
                     VSKGPALDVLKIEPELEELLSQEIIGLVQNQPGSSKAFTIDIKRFINQLITPGQDTAA
                     SCIEIFERKEGDRGQGEGGSSKENPQKAVRVVGTAVFRKDKLVGFLDDSETKGFLYVT
                     GRAKQGIISLSLHEHAKKDVAFAMTRTSSRIIPKIDDDGVSFIVDIHAEGDLQQHEDT
                     EPVASPEIIKAIEEKAAQEIKALVEKALNKAKKDFGADIFGFGRTLHKRYPQIWKGLE
                     DDWREIYPDIEVTVKAEAKIRRTGMLTDTPKIH"
BASE COUNT         1223 a         1333 c         1139 g         1671 t
ORIGIN      
        1 ataaactcgg taaactcccc gttaaagccc ttcttgaatc tgtataagcc gtaaagtgga
       61 ttgtccgatg agaggtctcc ggacacgccc cgaaagtcat acatggtgca gttgttttcc
      121 tttgcccatt ttatcatttc ccactgaagg gcgtagttgg gcatgacgtt tcgatggctg
      181 ttagatgatg cgccgtatat ataccaggcc ttgtctccga aaataaaggc aagagtcccg
      241 gcaatgtact ttccttcgta ttctgccatg aaaagccggg ccagtccctt ttccaccagc
      301 tcgtcccaca gtgtctcaaa gtagtcaaaa cccctgatta ggaacttgtc cctgatgcag
      361 gtttcctcaa ggatgtcgta aaagggcttc agatcctctc gctctcctac ttttaccgta
      421 acgcccctgc gggcggccaa tcggatgttg tagcgggtct tgtgatgaaa gcccgccatt
      481 atctcatcta gggacttatt atttatatct aatcgaaaaa cgaatttggg ttgaatgccg
      541 tcaaagtcct tgcccgaatc cacatgggta aatcccacag aacgaaggtt tttgaccacc
      601 ctttcatcag gtgcttttat gtcagggtcg attttcaaaa agacggaacc atgttttttg
      661 gcaatggctt ttacctgggt aaagagaaat tttagagttt tttcatccga gtaatcggcc
      721 accgggcccc tcggagcata gaagatgcat ttattaaatc cgggaattgg aagtcttcgc
      781 tttaatatgg aaatccctgc cacgggcttg ccaccgtcca ttacaaggag cctaataggt
      841 tcccagcctg tgcggcgctt tacttcaccc cactcatagg attgaagaat atggcccttc
      901 ggtccccacg ccatgaactc gttgaacagc tctttctcct cttgttttat gatttttatt
      961 tccattatga tgtacaacct ccgccgtgtt ttttgctttc ttctttatta ttatacatga
     1021 tttaggcgtg gattaaagga aatgttattg gatttttatg ccttccatca cctgggccag
     1081 tctaccaaca tcctctttta tttcctccac ggaaatggcg atttcgccca gcaatacgtg
     1141 ctgtatctcc acatgttcgg ctatttcatg cacccggttg gaagcatcta tgctgatgtc
     1201 ggtcatctgt tggaagtact ggctggtatt ttcggctgat gcagacatct gctgggttgc
     1261 cgcggaaatc tcctgcatct ggtgggcaac atttgtgaca gcgtctaata ttttttcaaa
     1321 agcagcggat gcatccttta cagcatttat gccctgttga acgttggccg agccttcatc
     1381 tttagctttt acggcttttt ccacttcatg tattaccgaa gtgattagct cgccgatttg
     1441 ctttgcagcc tcgccggact gctcggctag tttccttatc tcatcggcta caacagcaaa
     1501 gccccggccc tgttcacctg ccctggcagc ctcaatggca gcatttagcg ccagaaggtt
     1561 tgtctggtcg gctatgccgg taatggtttc gacaatttga cctatagtct cagaactttc
     1621 tcccaatgat tttattacgg ttgatgtgta cctattggat tcctgtattt tctccatctg
     1681 ctccgcgact ttgtccatta gagtttttcc ggaattggcc atttcggacg tatctgcggc
     1741 atttattgcg gcctccgagg agctggcagc aatctgctcc actcccgcac tcatttgaga
     1801 tagcatgtcc gaaatgttgt ccaccaactt ttgcatctcg cttaggcttt cggagctctt
     1861 ttctatgctt tccttcatag agccttcgta tgaagatgag gcttggtgac tttgcttggc
     1921 catactgtcc atcaagtcgg cagaagacaa tatctcatca gttatgtcct tggcgctttt
     1981 taagatgctt tttatccttt ccaagctgtt attatataga tctgccagtt tgcctatctc
     2041 accaccggca gttttcttga ttttgacggt aaaatcacct ttggacatgg cttcaaagct
     2101 ctggacaatc ttatcaacgg gaagagcagt ttctcttgtt atgagaaaag atacggtgcc
     2161 tgaaattaaa gctgctatcc ctatcgccag tagattcatg taattagcaa agtcttctcc
     2221 gtggaaatag tatgtatacc ctcctaaaga ggctgctact agtatcatcg tgacaattgc
     2281 aactctagct cgtatgtttt tcataattgc cctcctctgg gtattttaaa caattataat
     2341 attataatta gacaaatgtt tatttttccc tgcttttttc taaaatattg tcgaaaagtg
     2401 gaaaaacaaa aaaagaaagg cctaaatcta attggccttc tttttgtagc ttgatatata
     2461 ataaatgtat tttttcagtc atcacttatt ttcctgcagc atgcagggct gccttgataa
     2521 agtgagtaaa gaggggatgg gggttattgg gccgggattt gaactccgga tgaaatacgg
     2581 tcgctacaaa ccatggatgg tctttgagtt ctatgatttc aacaaagttt tcctcggggg
     2641 atattcccga aaggaccata ccgcacttac taaggctgtc cctgtaagca ttgttaaact
     2701 catagcggtg gcggtggcgc tccattatta tttccgtgcc gtaggctacc cttgctatgg
     2761 tgttttccga cagtttgcag gcaaagctcc ccaggcgcat ggtcttatca tctatgcagt
     2821 ttgtctttgg aaaatctatc acaggatttg gggtatcagg cattacttca gtagtatggg
     2881 catcttttaa accgcataca ttttgtgcaa actcgactac ggcacactgc atgcctatcc
     2941 ctatgccgaa aaagggaatc ttgttttccc gggcgaactt tatggccctt atcattccct
     3001 cgattccacg ctggccgaaa ccgccgggga caatcatgcc atctatatta ttaaaggtgc
     3061 ttgctaaatc cacatcattt tcctctagct tttccgagtg aacccatttt atgtttacct
     3121 ttacatcgtt ggctatgccg ccgtggcaaa gggcctcggc cacgctcagg taggcatcgt
     3181 ggagctccac gtacttgccc accagggcta tgtttagctc ttttgtgggg tttttgattt
     3241 tcctcactat ctcttcccac tccgaaaggt cggtcttcat gccgtctacg cccagtttct
     3301 tggctacaat ctggtctagg ccttcgtttt tgaggatgag gggcacttca taaatggttt
     3361 cggcatcgta gttctgaatg acacactccg gctccacatt gcaaaagagg gcgatctttt
     3421 cctttacatc ttgcgataag ggacgttccg agcggcagac tatgatatcg ggttgtatac
     3481 cgatgctccg cagctccttt acgctgtgct gggtgggctt ggtttttaat tcccctgctg
     3541 ttgaaatata gggaactagg gttacatgaa tgtacaggac attatcccga cctacttcgg
     3601 tcttcatctg ccttatggct tccagaaagg gcaggctttc gatatctccc accgtgccgc
     3661 cgatttccgt tattactata tcggccttaa attcctctcc caccttcctg atccgctctt
     3721 tgatttcatt ggtaatatgg ggtatcacct gcaccgtttt gccgtcatag cctccctgcc
     3781 gttccctggt aatgaccgac aggtacacag cccccgtagt tgtatcgctg ttcctggtga
     3841 ggtttatatc tacaaatcgc tcgtaatgtc ccaggtctaa atccgcttct ccaccgtcat
     3901 ccgtcacaaa tacttccccg tgctgataag ggctcatggt gccggggtcc acattaatat
     3961 aggggtcaca tttctgcatg gtgacagata gacccctact ttttaaaagc cttcccaggg
     4021 aggctgcagt aatgccctta cccaaggatg acacaactcc tccagtgaca aatatgtatt
     4081 tggtagacat ttataaaatc ccccttaatg ttctccgagc acacaatctc attacacctt
     4141 attttatcca tgggcgcatg tacttgtcaa tagtcgcaaa ctattatttt agcatgttgt
     4201 caacggcttt aaagatgggc tcaataacgt attgggtcag cttagtggga tttaatatcc
     4261 ctagcttgaa tatatggatt atgccgtagg caatgccgat tatcagcagg ccgcaaaaaa
     4321 ccgccagttc ccgccacacc cgacttttta taagacctgg tagctccaac atcaagatta
     4381 tgacaaccga tataatcaac agcgccaatc cgatcaccgc tttaaggttt aagtttaatg
     4441 gatttttggt gtatccgtaa gcatgccggt ccggcggatt ttggcctccg ctttcaccgt
     4501 cacttcaata tccgggtaga tttcccgcca atcatcctcc agccctttcc agatctgagg
     4561 atatctttta tgtaaggtcc tcccgaaacc gaagatatcc gctccaaagt cttttttggc
     4621 cttgtttaag gccttttcaa ccaaggcttt gatctcctgg gccgcctttt cctcaatggc
     4681 ttttatgatt tcagggcttg ctacgggttc tgtatcttca tgctgttgca ggtctccttc
     4741 cgcgtgtata tctacaataa aagaaacccc atcatcgtct attttcggta ttatcctgct
     4801 gctggtcctt gtcatggcaa aggccacatc ttttttggca tgttcgtgta agctaaggga
     4861 aattatcccc tgctttgccc ttccggtgac atataaaaag cccttggttt ccgaatcgtc
     4921 caggaagcct accaatttgt cttttctaaa gactgctgtg cccaccaccc gaaccgcctt
     4981 ttggggattt tccttggatg agccgccttc cccctggccc ctgtctcctt ccttgcgctc
     5041 aaagatttct atgcaggatg ccgctgtatc ctggccggga gtaatcagtt gatttataaa
     5101 gcgctttata tcaatagtaa aagccttgct tgacccaggt tgattttgca caaggccaat
     5161 gatttcctgg gatagcaatt cttccaattc cggctctatc tttagaacat ctaaggccgg
     5221 tccttttgaa acaagtaccc aggtgtttaa gcgaatatcc tttgaacgca agaggacgtc
     5281 tatgagctgg tctacgcctt cccgggccaa atcctcgccg attattacga tgtttgaatg
     5341 ggcaatatat tctcggcggg gaaggc
//
LOCUS       DAONUO010000124         5366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11223_length_5366_cov_3.465072, whole genome shotgun sequence.
ACCESSION   DAONUO010000124 DAONUO010000000
VERSION     DAONUO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11223_length_5366_cov_3.465072"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..695)
                     /locus_tag="PK768_05640"
     CDS             complement(195..695)
                     /locus_tag="PK768_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQA60486.1"
                     /translation="MAHDLIRVIKERRSIREYLPKPIPREVLEDILDCGRLAPSARNI
                     QPWLFVVSTEKEIKEGIAKLTLYGKFIANAAACISVFCERDSKFLVEDGCAATQNMII
                     AAKAYGVDSCWVAGYERPYSEDIRELLGVPEKYKLISLISLGYSDKSASRPKKTLNEV
                     VRWEKY"
     gene            complement(712..3846)
                     /gene="ileS"
                     /locus_tag="PK768_05645"
     CDS             complement(712..3846)
                     /gene="ileS"
                     /locus_tag="PK768_05645"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQA60487.1"
                     /translation="MFKKVEPTMNFVPIEEKILDFWNREQVFKKSIENRKDAPNYVFY
                     EGPPTANGLPHAGHVLTRVVKDLIPRYKTMTGHRVDRKAGWDTHGLPVELEVEKQLGI
                     SGKPEIEEYGVEEFIKKCKASVFTYEQEWRKMTERVGFWIDMDDPYVTYHNTYIESVW
                     WALKSIWEKGLLYKGNKVVPYCPRCGTSLSSHEVAQGYKEVEDPSIFVKFPLAGEEDT
                     YFLVWTTTPWTLPSNVALAVKDNYTYVKVEHNGEKLILAESRLGVLDGEYTLLESFPG
                     SKLASIKYEPVFPFFEDEKDRAFYVVTADFVSLDEGTGIVHIAPAFGEDDSLIGQKYD
                     LPVVQPVDAEGKFTEEVAPWSGVFVKEADKGILKDLRNRSILYKSERYKHTYPFCWRC
                     DTPLLYYGRSSWFIKTTAIRDKLLEINKGINWHPEHIRDGRFGNFLENVIDWCLSRER
                     YWGTPLNIWVCDDCGCQHAVGSIQELKDMSKEAIEDIELHKPYVDDVILKCPKCSGDM
                     KRVPEVIDCWFDSGAMPFAQFHYPFENKDYFEKHFPADFISEAIDQTRGWFYSLLVIS
                     TLLFDKSPYKNCLVMGHVLDEHGIKMSKHKGNVLDPWKVLNEQGADAMRWYLYSASPP
                     WSPSRFYQDAVSESQRRFLGTLWNVYSFFVLYANIDGFDPKKYDMAYNNRSEMDRWLL
                     SKVNSTNKKVRRDLDNLDITGAARTLESLIDDISNWYVRRSRERYWKSEMDDDKIAAY
                     LTLYEALVSFIKMAAPFVPFVTEEIYQNLVRSVYPDADHSIHLCDYPEINEELIDETL
                     EHKMKLARKIVELGRAARNKGKIKNRQPLQKMMVQLRNFDDKELIMDLSDIIKEELNI
                     KEIEYIDVAEEYFTYTVKPRFDLLGPKYGKLMSAIAKAISSAKATELLADARKNGQVT
                     IRVDGQDITILENELDIRAFDKEGFCAEGEGGYYVVLDTTITPELLLEGIARELVSKI
                     QNMRKEAGFEVEDKIYLYCVGDETIAKVLEVHGEEIKADTLALAIETSLAEDGYTREW
                     DLNDHKVTISVKKA"
     misc_binding    complement(3969..4193)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4402..>5366
                     /locus_tag="PK768_05650"
     CDS             4402..>5366
                     /locus_tag="PK768_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQA60488.1"
                     /translation="MAKKKTKKGRKILGYIIISILILIAMGFGAGLGLFLNYVKETPE
                     FDPTKLQTSETSMVFDQNGQLVAELHGEQNRIPVPLEKIPQDLINAFIAIEDQHFYSH
                     KGINIRSIIGSFIINLRHGDIKRGASTITQQLVKTAFLSPEQTWKRKAQEAWLAIQLE
                     RYYTKDEILEFYLNRIYFGHSAYGVESGADTYFGKSVDELTLAESAMLAGITKGPEYY
                     SPYNNFERAKERQEIILNTMAELGFITEEEAERAKQEEIKLVGLKNVAADYKAPYFTD
                     YVIQELAGLLQKELGLSETEAYNKIYTGGLKIYTTVDMEIQEAAE"
BASE COUNT         1385 a         1290 c         1122 g         1569 t
ORIGIN      
        1 attgaaggga tgaatatcct ttaataccgt tctcatgcga ccctctccat tctaaaaact
       61 atccgtcctc aaagaacaga acttatcgac aaggacctaa ccaaacactc gattggagac
      121 tctctcagtt ctcgggaagc ttccagcttg ccaaaaaaca gcttccaaaa aaacttggaa
      181 gctgtttttt tacttcaata tttctcccat ctgacaactt catttagtgt ctttttcggc
      241 ctgctggcac ttttatcgga ataacccagg gaaatgagcg atattaattt atacttctca
      301 ggaactccca agagctccct aatgtcttca ctgtacggcc tttcatatcc tgcaacccaa
      361 caagagtcga caccgtacgc cttggctgct ataatcatgt tttgagttgc ggcacatccg
      421 tcttccacaa gaaacttact atctctctcg caaaaaacgc ttatgcaggc agctgcattt
      481 gcgatgaatt taccgtaaag ggtgagttta gctatccctt cttttatttc tttttccgta
      541 gagactacaa acaaccatgg ctgaatattt cttgcgctag gcgcaaggcg gccgcagtct
      601 agaatgtctt ccagcacttc cctaggtatg ggtttgggaa ggtactccct aatgctcctc
      661 cgttccttta tgaccctaat taggtcatgt gccacaagac atcactcctc tttaagcttt
      721 ttttacactt atcgtgacct tatggtcgtt taaatcccat tcccttgtgt agccatcttc
      781 agctagtgaa gtttcaattg ctagggccag agtgtccgcc tttatctctt caccgtggac
      841 ttccagcact tttgctatgg tttcgtctcc gacgcagtat agatatatct tgtcttcaac
      901 ttcaaagcca gcttctttac gcatgttctg tattttgcta actaattccc tagcgatgcc
      961 ttctaacaag agttcgggag ttatcgtggt atcgagcacc acgtaatagc ctccttcgcc
     1021 ttcagcacag aagccttcct tgtcaaacgc cctgatatca agctcatttt ccagtatcgt
     1081 tatatcttgg ccatcaaccc ggatggtaac ttggccattt ttcctggcat cggccaacag
     1141 ttcggtagct ttggcggaag aaattgcctt ggctattgcc gacatgagtt ttccgtattt
     1201 gggaccgagc aagtcgaatc tgggtttgac agtatacgtg aaatactctt ctgctacatc
     1261 tatatattct atttccttga tatttaattc ctctttgatg atatcagata ggtccattat
     1321 caattcttta tcatcaaagt ttctaagctg caccatcatc ttttgaagtg gctgacggtt
     1381 tttgatttta cccttgtttc tggcagccct gcccaattct actatctttc tggccagttt
     1441 catcttgtgc tccagtgtct catcaataag ttcctcgttt atttccggat aatcgcagag
     1501 gtgaatgcta tggtctgcat cggggtaaac cgaccggact aaattctggt atatctcttc
     1561 ggtaacaaag ggcacaaagg gtgccgccat ttttataaag ctgaccaagg cctcatacaa
     1621 ggtcaaatat gccgctatct tatcatcgtc catttcggat ttccagtagc gttcccgtga
     1681 gcgacgcacg taccagttgc tgatgtcgtc gatgagggat tccaatgtcc gtgcggcgcc
     1741 ggtgatatcc agattgtcca aatcccgccg caccttttta tttgtgctgt tgacctttga
     1801 cagcaaccag cggtccatct cgctcctgtt gttataagcc atatcgtact ttttggggtc
     1861 gaagccatcg atattggcgt acagcacgaa gaaggaatat acgttccaca gggttcccag
     1921 gaacctgcgc tgggattcac ttacagcatc ctggtaaaag cggctgggac tccagggagg
     1981 acttgccgag tacaggtacc agcgcatggc atcggcaccc tgttcgttta gtaccttcca
     2041 tgggtctaga acattgccct tatgcttgct catctttatt ccgtgctcat ccaagacatg
     2101 acccattacc agacaattct tatatggaga tttgtcaaag agcagagttg agattaccaa
     2161 gaggctgtaa aaccatcccc tagtctggtc aatggcctcg gagataaaat ctgccggaaa
     2221 atgtttttca aagtaatcct tgttttcaaa gggataatga aattgagcaa agggcatggc
     2281 accggagtcg aaccagcagt cgataacctc gggcacccgc ttcatgtcgc cgctgcattt
     2341 aggacattta aggataacat catccacata gggcttgtga agttctatat cttcaatagc
     2401 ttccttgctc atgtctttta attcctggat actacctact gcatgctggc agccgcaatc
     2461 atcgcatacc cagatattaa gcggcgttcc ccaatatctc tccctgctca aacaccagtc
     2521 tatgacgttt tcaaggaaat tcccaaagcg gccgtcacga atgtgctcgg gatgccaatt
     2581 tatccccttg tttatttcaa ggagcttatc ccttattgca gtggtcttta taaaccagct
     2641 gctgcgcccg tagtacaaaa ggggagtgtc gcagcgccag cagaagggat aggtgtgctt
     2701 gtatctttcc gatttataaa gaatgctgcg gttcctcaga tctttcagta tgcccttgtc
     2761 tgcctctttg acgaagacac ctgaccaggg agctacttcc tcggtgaatt taccttcggc
     2821 gtcgacaggc tgaacaaccg gcaaatcata tttttggccg atgagggagt catcttcacc
     2881 aaaagcagga gctatgtgca ctatgccggt tccttcatct aggcttacga aatctgccgt
     2941 cacaacgtag aaggccctgt ctttttcatc ttcaaagaat gggaataccg gttcatactt
     3001 gattgaagct aatttgctcc cggggaaact ctctaaaagg gtgtactcgc catctaatac
     3061 tcccagcctg ctttctgcca gaatcagttt ttcaccattg tgctctacct taacgtaagt
     3121 gtaattgtct ttaaccgcta gggcaacatt ggacggcaag gtccagggcg tcgtggtcca
     3181 cacgagaaaa tatgtgtctt cctctcctgc aagcggaaat ttaacaaata tggagggatc
     3241 ttctacttct ttatatcctt gtgcaacctc gtggctggaa agggatgttc cgcagcgggg
     3301 gcaatagggg acgactttat ttcccttgta cagcaggccc ttctcccaaa tacttttaag
     3361 ggcccaccag accgattcga tgtaagtgtt atgataggta acatagggat cgtccatgtc
     3421 gatccaaaag ccgactcgct cggtcatctt gcgccattct tgttcgtaag taaatacgct
     3481 ggccttgcac ttttttataa attcttctac gccgtattct tcaatttcgg gcttgccgct
     3541 gatgcccagt tgtttttcca cttcaagttc tacgggaagg ccatgggtat cccatcctgc
     3601 ctttctgtct accctgtggc cggtcatggt cttgtacctt ggaatcaagt cttttaccac
     3661 ccgtgtgagg acgtggcctg catggggcag tccgttggca gtgggagggc cttcataaaa
     3721 aacgtaattg ggcgcatcct tgcgattttc tatgctcttt ttaaatactt gctccctatt
     3781 ccaaaaatct aggattttct cttcaatagg aacgaaattc atcgtgggtt caactttttt
     3841 aaacatctgc atcctcctgt cctaagttaa cttagagaaa aatctatact taaatcctta
     3901 ccgctaagag cccgcccgaa aacttgcggt atcatcctgg caattcagag ataaaaaaat
     3961 cccgtcccaa aagggacgag attgctcccg cgttaccacc caaatagtaa aagacataaa
     4021 atcgcctttt acctcttcat taggataacg gctaggtgcc ggctgcttct actccctcgg
     4081 tttcaaagca gcactcaggg gagatcttca gtcaccatca cctatcgggc ttccaccatc
     4141 cccgattcgc ttaaaggctt cctgatgcct actggcccca tcatcgcttt gcttgctgtt
     4201 aagttaacaa attatttctt tattatgata tattatttaa tacgcaatgt caacgatttt
     4261 cccgtagcag ttggaagcac atggtttttt cccacagttt taatatgttt ttatgtgtga
     4321 tataatgttt ttatccgaaa gattttatgc ttttcccgaa attcacatta acccatttac
     4381 aggaggatcg ggggactaat aatggcaaag aaaaaaacta aaaaagggcg aaagatacta
     4441 ggttatataa tcatctccat tctgatactt attgccatgg gttttggggc aggcttaggt
     4501 ttgtttttaa actatgtaaa ggaaactccg gaatttgatc ccacaaaatt acaaacaagt
     4561 gaaacttcaa tggtctttga ccaaaacggc cagctcgttg ccgagcttca cggtgaacaa
     4621 aaccgaatac ctgtgccgct ggagaaaatt ccccaagatt tgataaatgc ttttatcgcc
     4681 atcgaagacc aacactttta ttcccacaag ggaatcaata tccgctccat catcggctct
     4741 tttatcataa accttcgtca cggcgatatt aagcggggtg ccagcaccat aactcagcag
     4801 ttggtaaaaa ccgccttttt gtctccggaa cagacctgga agcgaaaggc ccaggaagcc
     4861 tggctggcaa tacagttaga aaggtattat actaaggacg aaatattgga gttttatctc
     4921 aatcggattt atttcggcca ctcggcttac ggtgttgaat cgggagctga tacttacttt
     4981 ggcaagagcg tagatgagct gaccttagcc gaatcggcaa tgcttgcagg cattacgaaa
     5041 ggcccagagt attattcacc gtataataac tttgaaaggg caaaagagcg gcaggagatc
     5101 attcttaaca ccatggctga actgggtttt ataacggaag aggaagccga gagggcaaaa
     5161 caggaagaaa taaagcttgt gggattaaaa aatgtggccg cggattacaa ggctccgtat
     5221 tttacagact atgtgattca ggagctggca ggcttattac aaaaagaatt aggcctgtct
     5281 gaaactgaag catacaataa aatttataca ggcggcttaa agatatatac tacagttgat
     5341 atggaaatcc aagaggcggc tgagaa
//
LOCUS       DAONUO010000125         5364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11230_length_5364_cov_3.468638, whole genome shotgun sequence.
ACCESSION   DAONUO010000125 DAONUO010000000
VERSION     DAONUO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11230_length_5364_cov_3.468638"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..354
                     /locus_tag="PK768_05655"
     CDS             19..354
                     /locus_tag="PK768_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQA60489.1"
                     /translation="MAKWQCEVCGYIFDGNEAPDNCPKCGAPKEKFQKLDDDAAALVE
                     RSRFSNNLHMELLTLLERAKEISAKGIEDALDPACVKIFEAMTTFAVDIQQRIKAELK
                     THMTKNKWG"
     gene            387..545
                     /locus_tag="PK768_05660"
     CDS             387..545
                     /locus_tag="PK768_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQA60490.1"
                     /translation="MQKWVCTICGYVYDPEVGDPEGGIDPGTAFEDLPDDWVCPECGV
                     GKDMFEEE"
     gene            601..1812
                     /locus_tag="PK768_05665"
     CDS             601..1812
                     /locus_tag="PK768_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA60491.1"
                     /translation="MNPIVVIGNGVASVAAVEAFRKYDKETPIIIFSDEPYHAYYRLR
                     LSSLIGDTLNLDKLYIRKPEWYRELNIQVRLDNKVIGIDTEKKSVVLENGDTVFFSKL
                     LIASGSSPFVPPVPGADLPGVFSIRSLDDVKRFNDYIANKNQGAVIGGGLLGLETAWA
                     LAKKGKKVHVIEGAPYILFNQLDEVAAKLLADLGKKASISFTVGGRLNKIVGNSRVSE
                     IHLADGQIMPVDFVVFATGVRPNIDLVNSTPILSSRGILVDELMRTSVEDVYAAGDIA
                     EYKGRVYGIWPVAQEQGKTAGLNMAGEKTSYNEIVPSNYLKVFDVEVFSVGDLCKEDK
                     ADATMKTLSEEDYVYRVVFFRDNTPVGAILFGDTKPATKIAKAIKLGIKIPDNIIRRG
                     DFQDFLNEISV"
     gene            complement(1973..3208)
                     /locus_tag="PK768_05670"
     CDS             complement(1973..3208)
                     /locus_tag="PK768_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HQA60492.1"
                     /translation="MTVTELINNRQYRINLLKEIVMELHRGKSVEEVKEKFREAVKDI
                     SHEEVAAMEQALIEEGLPVEEIQKMCDVHASIFKESLDKNPDPEMIPGHPVHTFFREN
                     RALEELMEKEIKPRLEAIKSADSREEQDKVVIELLEKYNLLMDIDKHYSRKENLIFPY
                     LEKYGITAPPKVMWGVDDEIRAEIKDVIKDLKNYDTDARLNIINKIEKNLERIQDMIY
                     KEEKILFPMAADNLTLDEWYQIYQESDEIGFCLVEPTAKWEPQNINLTERADIVADHH
                     TKGSIKFDTGILTPKQIELVFNHLPFDLTFVDKNGLVKYFSAGPERIFTRTKAVIGRS
                     VQNCHPPASVHIVEKLVQDFKEGKKDVEEFWINMGGKMIHIRYFAVRDENGEFIGTLE
                     VTQDITKIKELKGEKRLVE"
     gene            complement(3288..4043)
                     /locus_tag="PK768_05675"
     CDS             complement(3288..4043)
                     /locus_tag="PK768_05675"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQA60493.1"
                     /translation="MNKYVRFDNDHKIAYGLLEEDKVILIDGCIFGKWEKTDEKYDLK
                     DVKLLAPCEPSKVIGVGINYKSVVNQKGDPMPEEPIIFLKPSSSVIGHDDEIVSPKGV
                     KELNYEAELVVVIKDKIKDVSEEDAKNHILGYTCGNDITAKDFLIKGKPWTKGKAFDT
                     FMPIGPCIAEGLDGDNLNIKMYHNGKLTQDGNTSDMIFNISQIISFVSHIMTLNPGDI
                     ISTGTPPGKGNLLPGDVIEVEVEGIGRLTNKVV"
     gene            complement(4075..5226)
                     /locus_tag="PK768_05680"
     CDS             complement(4075..5226)
                     /locus_tag="PK768_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQA60494.1"
                     /translation="MDFGFYMPAQLIFGPGKAKETGSYVKALGKKALVVTGKSSAKKA
                     GYLDLITKSLEKEGVDWVLFDEIEPNPLTTTIDKGISFARENKVDVVIGLGGGSAMDS
                     AKCIAFGVNKEDSIANYFGAQPAGEVLPIALITTTAGTGSEANNFAVMTNPETNVKKA
                     LSSPNTYASISIIDPELMLTVPKKVTASTGIDVFFHSMESYLTKKSHPLSRPLSLEGM
                     RLVAENLKGAYDDGNNIEYRERMAWANTIAGIAINLAGNCGIHALGHPLSAYYDLPHG
                     ETLAAVSLAFMKFSINEAAEKLAEIAKILGVKTEGLSVVEAAEKGIEAFEVFLKSVDL
                     PTKISDLGITEDSIEKLALNAYEYSSHNFVASPREMTLEDAINLYRESL"
BASE COUNT         1407 a         1162 c         1126 g         1669 t
ORIGIN      
        1 aaaaggaggt tgtctataat ggcaaaatgg caatgcgagg tgtgtggtta catatttgac
       61 ggcaatgaag cacctgataa ttgccccaag tgcggtgcac ccaaggaaaa attccaaaaa
      121 cttgatgacg atgctgccgc cttagttgaa agatcgagat ttagcaacaa tctacatatg
      181 gaactattga ccttactcga aagagcaaag gaaattagtg caaaaggtat tgaggacgcc
      241 ctcgaccccg cttgtgtgaa aatctttgaa gccatgacaa cttttgcagt agacattcaa
      301 cagagaatca aagcagagct taaaactcac atgaccaaaa ataaatgggg ctaaggcctt
      361 gatttaggaa ataaggagga aaaaacatgc aaaaatgggt ttgcactata tgtggatacg
      421 tctacgatcc agaagtgggg gatcctgaag gaggaatcga cccgggaaca gcatttgaag
      481 accttcccga cgactgggtt tgccccgaat gcggtgtcgg caaagatatg tttgaagaag
      541 aatagactta aggcccgata ttcgggcctt attgtgtgca ataaaaatgg gggtgaaaac
      601 atgaatccaa ttgttgtaat aggcaacggc gttgcttctg ttgctgctgt tgaagccttc
      661 agaaagtatg acaaagaaac cccgattatc atatttagcg atgagcctta tcatgcctat
      721 taccgcttgc ggctatctag cttgatcggt gataccctaa atttagacaa gctatatatc
      781 cgcaagccgg aatggtaccg cgaactaaac atacaagtgc gcctggacaa taaagtcatt
      841 ggcattgaca ctgaaaaaaa atcggttgtc ctcgaaaatg gcgatacagt tttcttttct
      901 aagttgctca tagcaagcgg tagcagcccg tttgttcccc cggttcccgg tgcggacctt
      961 cccggtgtat tttccatacg gtctttggac gatgtgaaaa gatttaatga ttatatcgct
     1021 aataagaatc agggagcggt aatcggcgga ggtctcttgg gtctcgaaac agcctgggct
     1081 ttggcaaaga aaggtaagaa ggtacatgta atagaaggag ctccctatat actctttaat
     1141 cagctagatg aagtcgccgc aaagctctta gcagatttag gcaaaaaggc tagtattagc
     1201 tttacagtcg ggggacggct aaataaaatt gtaggaaata gccgagtctc ggagattcac
     1261 ttggctgatg ggcaaattat gccggtggat tttgtagtct ttgccaccgg tgtccgccca
     1321 aacattgacc ttgtaaatag tactccgata ctaagttcaa ggggcatcct ggtagatgag
     1381 ttgatgcgaa cttcggtcga agacgtatac gcagcaggtg atatcgcaga gtacaagggg
     1441 cgggtctatg gcatctggcc agtagcccag gaacagggca aaacggctgg ccttaatatg
     1501 gctggagaaa agacttcgta caatgaaatc gtaccctcga attacttaaa ggtatttgat
     1561 gtggaagtat tttcggtagg tgacctgtgc aaagaggata aggccgatgc tacaatgaaa
     1621 actttaagcg aggaagatta cgtttataga gtggtatttt tcagagacaa cactcccgtg
     1681 ggagcaatcc tctttggcga tacgaagcct gctacaaaaa tcgcaaaggc catcaaattg
     1741 ggaattaaaa ttccggataa tattataaga agaggggact ttcaagactt tcttaacgaa
     1801 atctcagttt aagtcatttc acatgccgaa tcgtcttctg ggcagaataa ctgacattta
     1861 ccacttgaga gacttgacct ttttgtatat tcaaatgcaa tataattaaa aatagcgtga
     1921 tgtcctgttg acatcacgct atttttgctc aacatccttt tatccctgcc tcttattcca
     1981 ccagtctctt ttcgcctttt agttctttga ttttagttat atcctgggtt acttctaggg
     2041 ttcctataaa ctcaccattt tcatctctta cagcaaagta gcggatgtga atcatcttgc
     2101 cgcccatatt aatccagaat tcctccacat cttttttgcc ctctttgaaa tcctgcacca
     2161 gcttttctac gatgtggaca ctggcaggtg gatgacagtt ctgaacgctt ctgccgataa
     2221 ctgcctttgt cctagtaaaa atcctttccg ggccggcaga aaagtatttt actaaaccat
     2281 tcttgtccac aaaggttaaa tcaaaaggta gatgattaaa gacaagctcg atctgtttag
     2341 gagtgaggat acctgtatca aatttgatgg aacctttggt gtgatggtct gcaacgatgt
     2401 cagctctttc agtcaaattg atgttttgcg gttcccactt ggcagtaggt tctaccaagc
     2461 aaaaacctat ctcgtcactc tcttgataaa tctgatacca ttcgtctaaa gttagattgt
     2521 ctgctgccat cgggaagagg attttttctt ccttgtaaat catgtcctga atccgttcaa
     2581 ggtttttctc tattttattt ataatgttta gcctggcgtc tgtatcgtag tttttaaggt
     2641 ctttgatgac atcttttatt tcagcacgga tttcatcatc cactccccac attaccttag
     2701 gcggtgctgt tattccgtac ttttcaaggt aggggaatat caggttttcc ttgcggctgt
     2761 aatgtttgtc aatgtccatg agcagattgt acttttccaa aagttcaatg accactttgt
     2821 cctgctcttc ccggctgtcc gccgatttta tggcctcaag tcggggcttt atctcttttt
     2881 ccattaattc ttccagggcc cgattttctc ggaaaaaggt gtgtacggga tggccgggaa
     2941 tcatctctgg atcgggattt ttatccagag attccttaaa aattgaggca tgaacatcac
     3001 acattttttg tatttcttca accggtaaac cttcttcaat gagggcctgt tccattgccg
     3061 caacttcttc atgggatata tctttgacag cttccctaaa tttttccttc acttcttcta
     3121 ctgacttgcc ccggtgaagt tccatgacta tctcttttag aagattaatc ctgtattgtc
     3181 ggttgttgat aagttccgtc actgtcatca cctctacgcc agtttttttg gttcaccttt
     3241 cattaataag catacctgta ttgcccggga aatatgtgag gctgccatta cactacttta
     3301 ttcgtcagtc ttcctatgcc ttccacttcc acctcaatca catcgccagg cagcaggttt
     3361 ccctttcctg gaggggttcc tgtacttata atatctcctg gatttagtgt cataatgtgg
     3421 gaaacaaaag atatgatttg ggaaatattg aatatcatat ccgaagtatt tccatcttga
     3481 gtgagttttc cattgtggta catcttgata tttaaattgt cgccatccaa gccctccgct
     3541 atgcaaggtc cgatgggcat aaatgtatcg aaagcttttc ccttggtcca gggttttccc
     3601 tttatcagga agtcttttgc agttatatcg ttgccacagg tgtagcctaa gatgtgattt
     3661 ttcgcatcct cttcactcac atcttttatt ttgtccttta tgactaccac cagctcggct
     3721 tcgtagttta gctcttttac acccttaggc gaaacaattt catcgtcatg cccgataacc
     3781 gagctacttg gctttagaaa gattatcggc tcttccggca tcgggtcacc tttttggttt
     3841 acaactgatt tgtagtttat tcccactcca atgactttgc tgggttcaca aggagccagc
     3901 agctttacat cctttaaatc atatttttca tcagttttct cccatttccc aaaaatgcag
     3961 ccatcaatta aaattacttt gtcttcttca aggagaccgt aagctatttt atggtcattg
     4021 tcaaatctca catatttatt cattttgtca ccttctgttg ttaaatttta cattttataa
     4081 agattccctg taaaggttta tggcatcctc aagagtcatc tcgcgagggc tcgctacaaa
     4141 attatgactt gaatattcgt aagcatttag tgccagtttt tcgatgctgt cttccgttat
     4201 gcccaggtcg gatatttttg tgggtaggtc tacacttttt aggaaaactt caaatgcttc
     4261 tatgcccttt tcggcagctt caacaacaga caagccttcg gttttgacac cgagtatctt
     4321 agcaatttca gcaagttttt cagccgcctc gttgatggaa aacttcataa aagctaagga
     4381 aaccgcggca agggtctcgc cgtgaggcag gtcatagtat gcgctaaggg gatggcctag
     4441 agcgtgtatt ccacaatttc cggcaaggtt tatggcaatg cctgcgatag tattggccca
     4501 agccatccgc tccctgtatt caatgttatt tccgtcgtca tatgcgccct ttaagttttc
     4561 cgctaccagg cgcattcctt caagggacag gggtctgctt aaaggatggc tttttttagt
     4621 gagataagat tccatgctgt ggaaaaatac atcaatgcct gtagaagcag ttaccttttt
     4681 aggaacggtc agcatcagtt cagggtctat gattgaaatg ctggcgtagg tgttaggaga
     4741 tgacagcgcc tttttcacat tagtttcagg atttgtcatg actgcgaaat tgttagcttc
     4801 actgcccgtc cctgcagtgg ttgttattaa agcaataggg agtacttctc ctgccggctg
     4861 ggcaccaaag tagttagcaa tagaatcttc cttgttcacc ccgaaggcta tacatttggc
     4921 agaatccatt gcacttcctc cgcccaagcc tatgacaaca tcaactttgt tttccctggc
     4981 aaaacttatt cccttatcga tggtagtcgt cagcggattc ggttcgattt catcaaataa
     5041 aacccaatca actccctcct tttctaaact ttttgttatc aaatcaaggt acccagcttt
     5101 ttttgcactg gatttgcccg ttactactag agcttttttc cccaaggcct taacgtatga
     5161 acctgtttct tttgcctttc cagggccaaa aataagttgt gccggcatgt aaaaaccaaa
     5221 gtccaattaa aacacctcca aataacttct tttgatattg cggctattta tccttccaac
     5281 ccatcctatg ggagattttt ttggcacagt cgatgacgct ttcggagatt tgatttattc
     5341 tttcttgcgt tacggtaacg gtag
//
LOCUS       DAONUO010000126         5336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11295_length_5336_cov_3.741526, whole genome shotgun sequence.
ACCESSION   DAONUO010000126 DAONUO010000000
VERSION     DAONUO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11295_length_5336_cov_3.741526"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PK768_05685"
     CDS             <1..800
                     /locus_tag="PK768_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQA60495.1"
                     /translation="LEEVAKFRAARRLWAKIMKERFKAENPKSMMLRFHTQTAGSTLT
                     AQQPDNNIVRVAIQALAAVLGGTQSLHTNSRDEALALPTEDSVRIALRTQQIIAHESG
                     VAETIDPLAGSYYVEYLTNTIEQKALEYIEKIDKLGGAPRAIEKGFIQQEIQNSAYGY
                     QMEVEQGKRIVVGVNKYQIEEESPKGLLKVDPSVRKLQEEKIRQLKATRDNDQVAVVL
                     QELRDTAETDENLMPVIIKCVKAYCTLGEICDALRTVFGEYKASINI"
     gene            814..1215
                     /locus_tag="PK768_05690"
     CDS             814..1215
                     /locus_tag="PK768_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQA60496.1"
                     /translation="MTGSKTIRVLIAKPGLDGHDRGAKVIARALRDSGMEVIYTGLRQ
                     TPEQIVEAAIQEDVQVIGLSILSGAHNFLFPRVMELLKEKDAEDILVIGGGVIPEEDI
                     PGLKAAGIKEIFTPGTPTSAIIEFIKNNVKA"
     gene            1236..2180
                     /gene="meaB"
                     /locus_tag="PK768_05695"
     CDS             1236..2180
                     /gene="meaB"
                     /locus_tag="PK768_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQA60497.1"
                     /translation="MDFVSRVINADKRSIARLITLIEEQSDEARNLLKQLYKFTGNAI
                     TIGITGPPGAGKSSIVNLLAKKLRESNKKVGILAIDPTSPFTGGALLGDRIRMQDLTL
                     DEGIYIRSMGSRGSLGGLSRASYDAVKVLDAAGMDYIIIETVGVGQSEVDIIKLADIV
                     ILVLIPGMGDDIQAIKAGIMEIADIFVVNKSDLDGTDRLVTEIQMNLDLNPDKSKSKP
                     PIVKTVALTGEGIDELKNTIFHTQKQFREQGLLNKKRKKRINQELRELIREIHLSQIA
                     SSFETKIDDFTEKIFNKEVDPISAAVAILQNSKDNIKP"
     gene            2236..2634
                     /gene="mce"
                     /locus_tag="PK768_05700"
     CDS             2236..2634
                     /gene="mce"
                     /locus_tag="PK768_05700"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQA60498.1"
                     /translation="MIQKIDHLGIAVKSIDAAMKVYTDILGLKVTGIETVEEQKVKTA
                     FIPVGESKIELLESTSPDGPIAKFIEKRGEGIQHIALRVDNLEAKLKELKEKGVRLID
                     EKPRIGAGGAKIAFIHPKDTKGVLIELCER"
     gene            2646..4199
                     /locus_tag="PK768_05705"
     CDS             2646..4199
                     /locus_tag="PK768_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQA60499.1"
                     /translation="METIEAKLKALQQKRAIIEMGGGEKRIQKQHESGKLTARERIEK
                     LLDKDSFVEIDAFVEHRSIQFDMQNTKAPGEGVVTGYGTVDGRLVFIFAQDFTTIGGS
                     LGEMHAAKICKVMDMANKMGAPLIGLNDSGGARIQEGVDSLKGYGEIFYRNTLSSGVI
                     PQLSVILGPCAGGAVYSPALTDFVFMVSGISKMFITGPQVIKAVTGEEVTPEELGGAT
                     AHNQKSGVAHFISQSEEKCFEQIRKLLSFIPSNNLEDPPYKATTDDPTKTVPELNTIV
                     PIDPNKPYDMKDIIKLVVDDNDFFEVQPLYAQNLITGFARLSGHSVGILANNPKHLAG
                     CLDIDASDKAARFIRFCDAFNIPLVTFTDTPGYLPGVDQEHGGIIRHGAKLLYAYSEA
                     TVPKLTIITRKAYGGAYIAMCSRHLGADQVFAWPTAEIAVMGPDGAANIIFKKEIESS
                     DDPISTRQQKIEEYRDNFANPYQAAARGYVDDIIEPAATRAKLVSALEMLMSKSEKRP
                     AKKHGNIPV"
     gene            4214..4582
                     /locus_tag="PK768_05710"
     CDS             4214..4582
                     /locus_tag="PK768_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HQA60500.1"
                     /translation="MTTAQVFQESIRTTIFGMGLVLLTLFVLSLILDLMRIIFAPRVN
                     IKKNEAIEAGDTAAKIQTENITSDSKDDTQLIAVITAALAAYLDTPISSIKVGTIRKI
                     HEKTPIWGMESRIYNINNKL"
     gene            4612..5007
                     /locus_tag="PK768_05715"
     CDS             4612..5007
                     /locus_tag="PK768_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HQA60501.1"
                     /translation="MKKYRITVNGQTYEVEVEEVGGNISQSVPVQEQTPVAKIEQPKP
                     QPQPKPEAKPKGSGTAGKNKVTAPMPGTILSIKKKVGDNIQKGDVIMILEAMKMENEI
                     IAPEDGTITSIDTSEGASVNTGDVLATFE"
     gene            5037..>5336
                     /locus_tag="PK768_05720"
     CDS             5037..>5336
                     /locus_tag="PK768_05720"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQA60502.1"
                     /translation="MVEQIVKFFESTGYTNVTFEQLIMILIACFLMYLAIVKQYEPLL
                     LVPISFGMLLANIPIANLMGEGKFLYWIYQGVKLNIYPPLIFLGVGAMTDFGPLIA"
BASE COUNT         1917 a          953 c         1164 g         1302 t
ORIGIN      
        1 tacttgaaga agtagcaaaa ttccgagcag cacggagatt atgggcaaag attatgaaag
       61 aaaggtttaa agcagaaaac ccaaaatcca tgatgctcag attccataca caaaccgcag
      121 gttcaacctt aactgcccag cagcctgaca ataatatagt cagagtggct atacaggctc
      181 ttgctgctgt actaggaggc acccaatcac tacacaccaa ttccagggat gaggctttgg
      241 cgctgcctac tgaagattcc gtgaggatcg ctttgagaac acagcagata atcgcccatg
      301 aaagtggagt tgcagaaacc attgacccat tggctggttc atattatgta gaatatttga
      361 caaatacaat agaacaaaaa gcgttggagt atattgaaaa aatagataag ttaggtgggg
      421 ctccaagagc tattgagaaa ggttttattc agcaagaaat tcaaaatagt gcatatggtt
      481 atcaaatgga agtggaacag ggcaagagaa ttgtcgtggg agtaaacaag taccaaattg
      541 aagaagagtc gcctaagggc ttacttaaag tagacccatc ggttagaaaa ttgcaagaag
      601 aaaaaatccg gcaattaaaa gccactcggg ataatgacca agttgctgta gtgttgcagg
      661 aattgagaga tacggcagaa actgatgaaa accttatgcc ggtaataata aagtgcgtca
      721 aagcttattg tactttgggt gaaatctgtg atgcattgcg cactgttttt ggcgaataca
      781 aggcttctat aaatatatga agaggtggag tcaatgactg gcagcaaaac tataagggtg
      841 ttaattgcca aacctggtct tgatggtcat gatagaggag ctaaagtcat cgcaagagct
      901 ttgagggatt cgggaatgga agtcatctat acaggcctaa gacaaactcc ggagcagata
      961 gtggaagcag ctatacagga agatgttcag gttatcggac tttctattct ttcaggcgcc
     1021 cacaattttc tttttccacg ggtaatggaa ctcctgaagg aaaaggatgc agaagatata
     1081 ctggtaatcg gcggtggagt aatacctgaa gaagatatcc ccggattaaa agcagcaggc
     1141 attaaggaga ttttcactcc cggaactcct acatctgcaa taatagaatt tataaaaaac
     1201 aatgttaaag cttaaaccga aaggtgtatt agacaatgga ttttgtttcg agagttataa
     1261 atgctgataa gagaagcata gcaagattaa ttacgcttat tgaagaacaa tctgacgaag
     1321 caaggaactt attaaaacaa ttatataaat ttaccggtaa tgccataaca attggaataa
     1381 cagggccacc cggtgcagga aaaagcagca tagtaaatct tttggccaaa aaactccgcg
     1441 aaagcaataa aaaagtagga atattagcta ttgacccaac gagccccttt acaggggggg
     1501 cgctattggg agataggatc agaatgcagg atttaacctt agatgaaggc atttacatac
     1561 gtagcatggg aagccgtgga agcctagggg ggctttcacg agcttcctac gatgctgtca
     1621 aagttctcga tgcagcagga atggattata taataattga gacggtaggt gtggggcaat
     1681 ctgaagttga tattataaaa ctagcagata ttgtcatttt agttttaatt cccgggatgg
     1741 gtgatgatat acaggcaata aaagccggca ttatggaaat agcagatatt ttcgtcgtaa
     1801 acaaatcaga cctagacgga acagaccgcc tggtaactga aatacaaatg aacttggact
     1861 taaatcctga taaaagcaag agcaagcccc caatagtaaa gacagtcgct ttgaccggcg
     1921 aaggtataga tgaactcaaa aatacaattt ttcacaccca aaagcaattt cgagaacagg
     1981 gcttgcttaa taaaaagcgg aaaaagcgta tcaatcaaga attacgggaa ttgatcagag
     2041 aaatccactt aagtcagata gcttcgtcat ttgaaactaa aattgacgat tttactgaaa
     2101 agatttttaa taaagaagtg gaccctataa gtgccgctgt tgcaatacta caaaactcaa
     2161 aggataatat aaaaccttga gaaatttttt gatatactca tacaaaacta aattacagaa
     2221 aaggagggat tagggttgat acaaaaaatt gaccatcttg gtatcgcagt caaaagcatt
     2281 gatgcagcaa tgaaagtgta tactgatatt ctaggcctta aagtcacagg aattgaaacg
     2341 gtagaagaac aaaaagttaa aactgccttt atacccgttg gtgagagtaa aatagagcta
     2401 cttgaatcca catcacctga tggacctatt gccaaattta tagaaaagcg gggagaaggc
     2461 atacaacata tagctttaag ggtagataat cttgaagcaa aacttaaaga gttaaaagaa
     2521 aaaggcgttc gtcttattga tgagaagcca agaattggag caggaggcgc caaaattgcc
     2581 tttatccatc ctaaagacac aaaaggagtc ttaatagaat tatgcgagag ataaggagga
     2641 acagtatgga aacaattgaa gccaaactaa aagccttgca gcaaaaacga gcaataattg
     2701 aaatgggcgg aggagaaaaa cgcatacaaa aacaacatga aagtggaaaa cttaccgctc
     2761 gggagagaat tgaaaaacta ttagacaaag acagttttgt agaaatcgat gcttttgtag
     2821 aacataggtc aatccaattt gacatgcaaa acaccaaagc cccaggagaa ggtgtagtaa
     2881 ccggttatgg caccgttgat ggcaggttag tatttatatt tgcccaagac tttacaacaa
     2941 taggcggttc acttggagaa atgcatgcag caaaaatatg taaagtaatg gatatggcca
     3001 ataaaatggg agcaccctta attggcctaa atgattcagg gggtgccaga atacaagagg
     3061 gcgttgactc tttgaaaggc tatggggaaa tcttctacag aaatacgcta tcatcgggag
     3121 ttataccaca gctttcagtt atactgggtc cttgtgcagg gggagccgta tattcacctg
     3181 ccctgaccga ttttgtcttc atggtatcgg gaataagcaa aatgtttata acaggccctc
     3241 aagttataaa agccgtaact ggcgaagagg ttacacctga agaactaggt ggagccacgg
     3301 ctcacaacca aaaaagtggc gtagcacatt ttataagcca aagcgaagag aagtgttttg
     3361 agcagataag aaagcttctc tcattcatac catcaaacaa tttagaagac ccgccataca
     3421 aagctacaac agatgatccg acaaagacag taccagaact caatacaata gttcccatcg
     3481 atccaaacaa accttacgat atgaaagata tcataaagct tgtagtagat gacaatgact
     3541 tttttgaagt tcagcctctt tatgctcaaa acttaataac tggatttgcg agactttcag
     3601 gccacagtgt gggaattttg gcaaacaatc cgaaacacct tgcaggttgc cttgacatag
     3661 atgcctctga caaagctgca agattcataa gattttgtga cgcttttaac atacctcttg
     3721 taaccttcac cgatacgcct ggatacctgc cgggagtaga tcaggaacac ggaggcataa
     3781 tcagacatgg tgccaaactc ttatatgctt attcagaggc caccgtgcca aagcttacaa
     3841 taattacacg gaaagcctac ggtggagcct atatagccat gtgttcaaga cacttaggtg
     3901 cggatcaagt ttttgcttgg cccacagccg aaatagcagt catgggcccc gatggagcag
     3961 caaacattat ttttaaaaaa gaaatagaaa gttctgatga cccgatatca actagacagc
     4021 aaaaaatcga agaataccgt gataactttg ccaacccata tcaagcagct gcaagaggct
     4081 atgtcgacga tattattgaa cctgctgcaa caagggcaaa actagtgtct gctctggaaa
     4141 tgctcatgag caaaagcgaa aagagacccg ctaaaaaaca tggcaatatt cctgtatagg
     4201 gaggcgattt aacttgacaa ctgcacaagt atttcaagaa tctattagaa ctaccatatt
     4261 tggtatgggc ttagtgttat tgacactatt tgtcttgtca cttattctcg acctcatgag
     4321 gataatattt gcacctaggg ttaatattaa aaaaaatgag gctatagaag cgggagatac
     4381 tgcggcgaaa atacaaacag aaaacataac aagcgacagt aaagatgaca ctcaattaat
     4441 agcagttatt actgcagcat tagctgcgta tttagatact cccatatcga gcataaaagt
     4501 aggtacaata cggaaaatac acgaaaaaac cccaatctgg ggtatggaat cgcgaattta
     4561 caacattaac aacaaacttt aatatagatg ttgaggaaag gagaacaaaa aatgaaaaaa
     4621 tatagaataa ctgtaaacgg acaaacttat gaagtagagg tagaagaggt tggaggaaat
     4681 attagccaaa gtgtgcctgt ccaagagcaa acaccagtag caaaaattga gcagcccaag
     4741 cctcaaccac aaccaaaacc tgaagcaaaa cctaaaggat caggtactgc tggcaaaaat
     4801 aaggttactg ctcccatgcc aggtacaatt ctgtcaatca aaaagaaagt aggagacaat
     4861 attcaaaaag gcgatgtgat aatgattctt gaagccatga aaatggagaa tgagatcata
     4921 gctccagaag atggcacaat aacctccatt gacacaagtg aaggagcttc tgttaataca
     4981 ggagatgtgc ttgctacttt tgaataaaat aagcaataaa ctggaggaga gaaactgtgg
     5041 ttgaacaaat agtaaagttt tttgaaagca ccggttatac aaatgtgact tttgaacaac
     5101 taattatgat acttatagct tgctttttaa tgtatcttgc aatagtaaaa caatacgaac
     5161 cattgctctt ggttcccatt agctttggta tgctgctcgc aaatatacct attgcaaatc
     5221 ttatgggaga aggcaagttt ctttactgga tatatcaggg agttaaatta aatatatatc
     5281 cacccctaat ttttttgggc gttggagcca tgactgactt tggaccatta attgct
//
LOCUS       DAONUO010000127         5332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11307_length_5332_cov_3.261891, whole genome shotgun sequence.
ACCESSION   DAONUO010000127 DAONUO010000000
VERSION     DAONUO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11307_length_5332_cov_3.261891"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK768_05725"
     CDS             complement(<1..413)
                     /locus_tag="PK768_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQA60503.1"
                     /translation="MKGLWEYLYHNDRRLFEYINKRIKCKVLDLFMPRITHLGGASFT
                     VLSCLFLYIFGQGETRLAATQAIVTVTSSQGIVQLFKNTVNRKRPYLVLPEVNTFWSR
                     LLKDHSFPSGHTTAGFSLAAVLALHFPNYGPVSYLL"
     gene            complement(441..851)
                     /locus_tag="PK768_05730"
     CDS             complement(441..851)
                     /locus_tag="PK768_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQA60504.1"
                     /translation="MMVVFDPGVRHRIMVCVTPQKSCKRLIEKGSARAKEMDGQFVVI
                     YVNNKKNLNRELKEHKILLELFELAKDFGGRVSILTGENVYDTLADFARENKITHIIV
                     GKSLRSAFEIQRHGEVINPLINAVEKMDITVEII"
     gene            complement(993..1625)
                     /locus_tag="PK768_05735"
     CDS             complement(993..1625)
                     /locus_tag="PK768_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HQA60505.1"
                     /translation="MIRYIVQRGDTLSQIAERFGTTVAAIMRANNLTNADLIFVGQVL
                     MIPVSEETPHTPEDCPPCPPTHPSPPSPPTPTPPPAGRPVVTRTFDGVEYTLSLNKSV
                     YRLGEQIIIRFRKKNILKVPLTLTYRTSQKVDFRITRDDRLIWQWSKGRVFTQAITSD
                     RLQPGEEIVYREVWNQRADGIITRPGVYRLTGWNLATPGVRLSLEFRITA"
     gene            complement(1915..4302)
                     /gene="lon"
                     /locus_tag="PK768_05740"
     CDS             complement(1915..4302)
                     /gene="lon"
                     /locus_tag="PK768_05740"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQA60506.1"
                     /translation="MGKASSNRTIPLLPLRGLLVFPNMVLHFDVGRDKSIGALEEAMV
                     ADQKILLVAQKDAKVDFPTRNEIYTTGTVAKIKQLLKMPQDTIRVLIEGVNRAVIEKY
                     IHTDPFFKVEIREVIESDQRADTQEEALMRSILNLFEHYMNMTKKLPPDIIVSLNNVT
                     NPGRFADIVASHLAIKVEDKQKILEMIDFKERLEILFAILSQEIEILEIEKKINYRVK
                     KQIEKNQKEYYLREQMRAIQKELGQQDERTAEAEEYREKILALDLKKDVEERIMKEID
                     RLEKMPNASAELSVIRTYLDWIVSLPWNVFTDDSVDIKKAEEILDEDHYGLSKVKERI
                     LEFLAVRKLSGKIKGPILCFVGPPGVGKTSLARSVARAMGRKFVRASLGGVKDEAEIR
                     GHRRTYVGSMPGRIIQGIRQAGSQNPVFLLDEIDKMSSDFRGDPSSALLEVLDPEQNN
                     SFSDHYLEIPFDLSRVLFITTANNLYSIPRPLRDRMEVVTIPGYTEDEKLEIAKRHLI
                     PKQLKEHGLNLTDVSFHEDALLKIIRNYTREAGVRNLERSIANICRKIARAVVEGEKN
                     TIDVDADLVEKFLGAPRYHYGTVEKQDLIGVATGLAWTEVGGDILNIEVSVMKGEGKL
                     ILTGKLGDVMQESAHASYSYVRSKAQLLNIDEDFHKKYDIHIHVPEGAIPKDGPSAGI
                     TIACALISALSMRPVDRTVAMTGEITLRGRVLPVGGIKEKVLAAHRAGIKTIILPEEN
                     TKDLEEVPQKVKDQLCFIFVNTMDEVIEKTLKPVPAEKPVNLEKSELDHYNIYPS"
     gene            complement(4399..>5332)
                     /locus_tag="PK768_05745"
     CDS             complement(4399..>5332)
                     /locus_tag="PK768_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQA60507.1"
                     /translation="RLIGATTRPPEEIPPAIRSRCLEIYFKPLMPEHIGIIIKNAVNK
                     IGFEIDDLSIKTIKKYTTNGREAVNIIQMAVGLATRENRKRIEARDVEWVINSGQYAP
                     RPERKVSPKPQVGLANGLAVYGPNMGILLEIEATAIKTEKGQGTLLVTGVVEEEEMGG
                     SAHRLRRRGTARGSVDNVMTVIRKYLGIDPRDYDIHLNFPGGTPLDGPSAGVAIAVSI
                     YSAMKNLVVNNYIAMTGELSIRGYVKPVGGVIPKIEAAKMAGAKAVIIPKENWQDSFK
                     TYDLNIVAVDRIEEVIEQVIFASEAEIRNETVSV"
BASE COUNT         1327 a         1312 c         1012 g         1681 t
ORIGIN      
        1 agcaaatatg atacgggacc gtaattggga aaatgcaggg ccagaactgc tgccaatgaa
       61 aaacctgcag tcgtatggcc ggatgggaag gaatggtcct ttaaaagcct gctccagaag
      121 gtattgacct cgggtaaaac caaatatggc cttttgcggt ttacggtatt cttaaaaagc
      181 tgtactattc cctggctgct ggtaaccgtg acaatggcct gggttgcggc tagccttgtc
      241 tcaccctgac caaaaatata caggaacagg catgaaagca ccgtaaagct agctccgcca
      301 agatgggtaa tcctcggcat aaataagtca agcaccttgc actttattct tttattgata
      361 tattcaaata agcgtctgtc attgtggtat agatactccc aaagcccttt caagaatatc
      421 ctcctcaagc cccagtatat ctaaataatc tcaaccgtta tgtccatttt ttccactgca
      481 ttaatgaggg gattgataac ctctccgtgc ctttgaattt caaaagcgga acgcaaagat
      541 ttgccaacaa taatatgcgt tattttgttt tcccttgcaa aatctgccaa tgtgtcgtaa
      601 acattttctc cagtcaaaat agaaacccta ccgccgaaat ccttagctaa ctcaaacaac
      661 tccaataaaa tcttgtgttc ctttaattct cgatttagat tttttttatt gtttacgtat
      721 atcactacga actggccgtc catctcttta gccctggcag atcccttttc tatcagtctt
      781 ttacagctct tttgcggtgt aacacatacc atgattctat gccgtacacc agggtcgaaa
      841 acgaccatca tggcaaaatc tcctctcttg tctttttcac caaccatttt tattatatat
      901 gaaatacatg ttttgcgcaa tgaacaaaag aaaacaaata atgggcgaat cccttatgga
      961 atcgccccta aagcgcattc tattatgctg gattatgccg ttattctaaa ttcgaggctt
     1021 agcctgacac cgggtgtggc cagattccac ccggttaatc tgtaaactcc cggtctcgtt
     1081 atgataccgt ctgccctttg attccatacc tcacggtaaa ctatttcctc tccgggttgt
     1141 aatctgtcgc ttgttattgc ttgagtgaaa actctccctt ttgaccactg ccaaattaac
     1201 ctgtcatcac gggttattct aaaatctact ttttgcgacg tcctataagt tagggtaagc
     1261 ggaactttta gtatgttttt cttccgaaat cttattatta tttgctcgcc aagcctatac
     1321 acgcttttgt tgagggaaag agtgtattcc acgccgtcaa aagtgcgggt aactaccggc
     1381 ctaccggctg gcggtggggt tggcgttggc ggtgagggcg gcgaaggatg tgtaggcggg
     1441 caaggagggc aatcctccgg cgtatgcgga gtctcttcag acaccggaat catcagtacc
     1501 tgccccacaa agattaaatc cgcattggtt aaattgtttg ccctcattat agcagctacg
     1561 gtagttccga acctttcagc aatttgagac agagtatctc ccctctggac tatatatcta
     1621 atcaaatctg tctccctcct ttaacacgta gttagctttt ttcagagatt agcaataatc
     1681 taatatatgt tatgtagtat ttattattat ggtgaagagt aattcaaata gagattaaac
     1741 cagcgtaacc ttttgaatat aactttggaa agcgctgcca aagtagaaaa gcgagtagac
     1801 tgttaaaagt atcgctcaaa gaaaaaagcc tccaaggatg tttttccccg gaagctattc
     1861 atttactctc aggttatgca tcgtgtattg cactgccctt tgcaaagtta aatcctatga
     1921 tggataaatg ttatagtgat ccaattcgct tttttctaag ttgacgggtt tttccgcagg
     1981 caccggcttt aaggtctttt caattacttc atccatggta tttacaaata tgaagcaaag
     2041 ctggtccttt actttttgcg gcacttcctc caaatctttt gtgttttctt caggcaggat
     2101 aatagtcttt attcccgccc tgtgcgccgc caaaaccttt tccttgatac ctccgacggg
     2161 aagcaccctt cccctcaagg ttatttcccc ggtcatagct acggtcctat ctacaggcct
     2221 cattgaaagg gccgatatga gggcacaggc gatggtaatt ccggctgaag gcccgtcttt
     2281 gggtatcgca ccttcgggaa cgtgaatgtg tatgtcgtat ttcttgtgaa agtcctcatc
     2341 gatatttaac agttgtgctt ttgacctcac atagctgtaa ctggcatggg ccgattcctg
     2401 catgacatcg cccagctttc ctgtgaggat gagcttgcct tcacccttca tgacggatac
     2461 ttcaatgttc aggatatctc cgccaacctc agtccaggca agaccagtgg ctacacctat
     2521 taaatcctgt ttttctacgg tgccgtaatg atagcgcggt gcgcctaaga acttctcaac
     2581 caaatccgca tctacatcta tggtattttt ttcaccttca actactgccc tcgctatttt
     2641 cctgcagata ttggcaattg aacgctcaag attccttacc cctgcttccc gagtgtagtt
     2701 tctgataatt ttaagcaggg catcttcgtg aaaagaaacg tccgttaaat tgaggccgtg
     2761 ttcttttaac tgtttcggaa tcaggtggcg cttggctatt tccagttttt cgtcttcggt
     2821 gtatcccgga atggtgacga cctccatgcg gtccctcagg ggccgtggta tactgtacaa
     2881 attgttggca gttgtaataa agagcaccct agacaggtca aagggtattt caagatagtg
     2941 gtccgaaaaa ctgttgttct gttcagggtc taggacctcc aataaggccg atgaagggtc
     3001 tcctctaaag tcagaactca tcttatctat ttcatccagt aagaatacgg gattctggga
     3061 acccgcctga cgtatcccct ggataatcct accaggcatg gaacccacat aggtcctcct
     3121 gtggccgcgg atttctgctt cgtccttgac cccgcccaag gacgccctca cgaattttct
     3181 acccattgcg cgggccacgg acctggccag ggaggttttt ccaactcccg gaggacctac
     3241 aaagcacaat atcggtcctt ttatttttcc cgacaactta cgcactgcaa gaaattctag
     3301 gatcctttct tttactttgc ttaaaccgta gtggtcttcg tctaatattt cttccgcctt
     3361 cttaatatcc acgctatcat ctgtaaatac attccatgga agggaaacaa tccaatccaa
     3421 gtatgtccta attaccgaaa gctccgctga ggcattgggc attttctcaa gacgatcgat
     3481 ttctttcatt attctttctt caacgtcctt ctttagatcc agtgcgagta tcttttccct
     3541 gtactcctca gcttctgcag ttctctcgtc ctgctgcccc aattccttct gtattgccct
     3601 catctgctct ctcaggtagt actctttttg gtttttttca atttgttttt ttaccctata
     3661 attaatcttc ttttcaattt caaggatctc tatttcctgg gataaaatcg caaaaagtat
     3721 ttccaaacgt tccttgaaat caatcatctc caaaatcttc tgcttatctt ccacttttat
     3781 ggccaaatgg gacgccacaa tatctgcaaa acgccccgga tttgttacat tgttaaggga
     3841 aacaatgatg tcaggcggca actttttcgt catgttcatg tagtgttcga aaaggttcag
     3901 tatactcctc attagggctt cttcctgggt gtctgccctt tgatcgcttt caataacttc
     3961 cctgatttca accttaaaaa aaggatctgt atgaatgtac ttttctatta cagccctgtt
     4021 gacaccttca atcaataccc ttattgtgtc ctggggcatc tttaagagct gttttatctt
     4081 agcgaccgtg ccggtggtgt aaatttcatt tctcgtggga aaatccactt ttgcatcctt
     4141 ttgtgccacc aacagtattt tttggtccgc caccattgct tcttccaaag caccgattga
     4201 tttatcccta ccaacatcga aatgtaacac catattggga aaaaccaata gtccacgaag
     4261 cggcaacagc ggtattgtcc gattagatga agccttcccc actttatccc cccccattcg
     4321 ggctattatt gaacaacaca tatgtttctt cttattctat tcgataaaat tttaataaat
     4381 ccttcaaaat attaaatgct aaacagatac agtctcattc ctaatttctg cttcagatgc
     4441 aaatataacc tgctctatca cttcctctat cctatcaacg gcaactatat tcaaatcata
     4501 agttttgaag ctgtcctgcc agttctcctt aggtattatt acggctttag ctcctgccat
     4561 ctttgcagcc tctatcttgg gtataacacc acctacaggc tttacatagc cgcggattga
     4621 aagttctccc gtcatggcaa tgtaattatt gacaactagg tttttcattg ctgaatatat
     4681 tgaaacagca attgccacac cagctgaagg gccatcaaga ggcgttcctc ccgggaaatt
     4741 taagtgaatg tcataatccc gaggatctat gcccaagtac ttcctgataa ccgtcattac
     4801 gttgtccacc gagcccctgg ctgttcccct tcgtctaagg cggtgggcgc ttccccccat
     4861 ttcttcttcc tctactactc ccgtcacaag caaggtcccc tgaccttttt ctgtctttat
     4921 ggctgtagct tctatttcca ataaaatacc catgttgggc ccgtataccg caagaccatt
     4981 tgcaaggccc acctgtggtt ttggacttac cttcctttca ggccttggtg catactggcc
     5041 gctatttatt acccattcca catctcgtgc ttcaatccgt tttctgtttt cacgagtggc
     5101 caaacctacc gccatctgaa taatgttgac tgcttccctg ccattggtag tatatttttt
     5161 tatggtttta atcgacaagt cgtcaatttc aaatcctatc ttatttacag catttttgat
     5221 gatgataccg atgtgttccg gcatcagtgg cttaaaatat atttccaagc atcttgaacg
     5281 aatagccggc ggtatttctt cagggggcct ggttgtcgca ccaattagcc ta
//
LOCUS       DAONUO010000128         5322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11333_length_5322_cov_4.078223, whole genome shotgun sequence.
ACCESSION   DAONUO010000128 DAONUO010000000
VERSION     DAONUO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11333_length_5322_cov_4.078223"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK768_05750"
     CDS             <1..241
                     /locus_tag="PK768_05750"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQA60508.1"
                     /translation="DIMDLKERLARIIVAYDREGNPVTAGDLKAQGAMAALLKDALKP
                     NLVQTLEGTPAFVHGGPFANIAHGCNSVIATKLAM"
     assembly_gap    244..253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <254..1062
                     /locus_tag="PK768_05755"
     CDS             <254..1062
                     /locus_tag="PK768_05755"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQA60509.1"
                     /translation="ADYVVTEAGFGADLGAEKFIDIKCRLAGLKPDAVIIVATVRALK
                     YNGGLPKSELDKEDLDALAKGIPNLLKHVENITQVFKLPAVVAINRFPQDTEAELKLI
                     EEKCNELGVNVALSEVWAKGGEGGIALAEELIRLTENSDGSQELTYAYDLDMPIKEKI
                     RAISQKIYGADDVVFTDKALREIANFEKLGFGNLPICMAKTQYSLTDDPKKLGRPTGF
                     NITVRDITISAGAGFLVALTGEIMKMPGLPKVPAAEKIDVNEKGVISGLF"
     gene            1080..1712
                     /locus_tag="PK768_05760"
     CDS             1080..1712
                     /locus_tag="PK768_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HQA60510.1"
                     /translation="MKFADMSCNEFVDRLASKSPVPGGGGVSALVGAIGTALASMVCN
                     LTTGKKKYAQFEDDIQRILKRAAELQDDLMKMIDEDAENFLPLSKAYGMPKDTEEERR
                     LKEETLEKALKQACEVPVKIVKACYEAIKLHAELVDKGSRLAISDVGVGVQCLRAAII
                     SGWLNIVININSIKDQDYVNKVREETENLVRQGAMLADKVYEQVEGILNK"
     gene            1898..2752
                     /locus_tag="PK768_05765"
     CDS             1898..2752
                     /locus_tag="PK768_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HQA60511.1"
                     /translation="MGILIKGKPVADKIKEKLKKEVAELKEKGITPKLAIIRVGDDPG
                     DMAYERGAIKTMVSVNIETDVRDLSKDISQDEFIKELKSINDDKSVHGILILRPLPDQ
                     LDENVIKNVIAPEKDVDCFSPVNVAKVFEGDETGFPPCTPSAVMEILKHYEIPLKGKN
                     AVVIGRSMIVGKPAAMLLLKEHATVTICHSRTKDLPKVASGAELLVVGIGRAKMIDSS
                     YIREGAVVIDVGINVDEDGKLCGDVDTDDCVDKASMITPVPGGVGSVTTSVLAKHVVK
                     ACKMQQAL"
     gene            2775..3440
                     /locus_tag="PK768_05770"
     CDS             2775..3440
                     /locus_tag="PK768_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQA60512.1"
                     /translation="MIISQHKPFEEIEELLRGCEKLVLVGCGQCATACMSGGEKELME
                     MKEELEGIDKKILTYIVPETSCNYLLVRRDLKKIKNALEEADAVLSFACGDGTQTIAK
                     LVNIPVYPGNNTLFVGEVERVGLYSEACRTCGDCVLGVTGGICPVTRCAKSLLNGPCG
                     GAKDGKCEVNPENDCAWILIYNKLKELGQLDKLTEIHEPKDYSRQAYPRNLNLRNQDG
                     GGR"
     gene            3437..4315
                     /locus_tag="PK768_05775"
     CDS             3437..4315
                     /locus_tag="PK768_05775"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQA60513.1"
                     /translation="MMSRLRKALESGEFAVTAELAPPKGTDFSHALQCAELFGDRVDA
                     INVTDFQSSSLKATSLAMCKALIDMGFEPVLQITGRDRNRLAIQGEMLSAGFFGVKNL
                     LALTGDHTTVGDNPGAKPVFDLDSVGILQAATMLANGVDMSGNKLEGSPSFYLGATVT
                     PEYDPIELQLVKMEKKIKAGAKFFQTQAVYDLSTMEKFKEMTRHLNTKILVGIIPLKS
                     AGMARFMNKNIPGINVPEHLIDRLKNSGDPVKEGIKIAGELIVELRDKNLCDGVHIMA
                     IGAEENVPLILDEAGL"
     gene            4335..>5322
                     /locus_tag="PK768_05780"
     CDS             4335..>5322
                     /locus_tag="PK768_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA60514.1"
                     /translation="MKVVVIGAGPGGYEAAIKAAKLGAKVTVVEKDNLGGTCLNRGCI
                     PTKALLASSDVFTTVKNATKFGVNVMGEVTADFPAMISRKDKLVSQLIKGIEFLFKKN
                     GIEVVYGTGKLIDKNKFEVTRDDGSKEILEADRIILATGSVPVCPPLFNYDGKNVITS
                     DEVLSLNKAPASIIIVGGGVIGCEIGQFLRRLGTEVTIVEMMPHILPMEDEDVANQLL
                     RQFKRDKIKVIAGKAITSVKVEENRVVAGLEDGELEAEIMMVSIGRKPYTDGLGLENV
                     GIQTDKAGRIPVNKKMETLVEGIYAIGDIVDSPFLAHVASKEGLIAAENALGG"
BASE COUNT         1641 a         1040 c         1438 g         1193 t
ORIGIN      
        1 cgatatcatg gatttgaaag aaagactcgc aaggatcatc gtcgcctatg accgggaagg
       61 caaccctgtc acagcaggtg acttaaaggc tcaaggcgct atggcagctt tactcaaaga
      121 tgccttaaag ccaaacctgg tgcaaaccct tgaaggaacc ccagcttttg tccatggagg
      181 tccttttgcc aatattgccc acggatgcaa ctcggtaata gctacgaaat tggcaatgta
      241 gttnnnnnnn nnntggctga ttacgtggta acggaggccg gcttcggagc agacctgggt
      301 gcagaaaagt tcattgacat caaatgcagg ctggcggggt taaagcctga tgcggtaatc
      361 atcgtggcaa cggtaagagc cctcaagtac aacggcggtt tacctaaatc cgagcttgac
      421 aaggaagacc tggatgccct ggccaaaggc atacccaatt tgttaaagca tgttgaaaat
      481 atcacgcaag tgtttaagtt gcctgccgta gttgccatca acaggttccc ccaggataca
      541 gaggccgaat tgaaactcat tgaagaaaag tgcaacgagc taggtgtaaa tgtggcactg
      601 tccgaagtat gggccaaagg cggtgaaggc ggcatagcct tggcagaaga attaattaga
      661 ctcacggaaa acagcgacgg cagtcaagaa ctgacttatg cttacgattt agatatgcct
      721 ataaaagaaa agataagggc aatttcgcag aagatatacg gtgccgacga tgtggtattt
      781 acggataagg cgcttagaga aatagccaac tttgaaaaac taggattcgg aaatctgccc
      841 atttgtatgg caaagaccca atattcactg acggatgatc ccaaaaaatt aggccggccg
      901 acaggattta acatcaccgt aagggatatc accatatcgg cgggagcagg attcttagtt
      961 gccctcacag gcgaaatcat gaagatgccg ggtctaccaa aggtgcctgc tgccgaaaaa
     1021 atcgacgtga atgaaaaagg cgtaatctcc ggcctgttct agttgaggag agtgatacag
     1081 tgaagtttgc agatatgtcc tgtaacgaat ttgtggacag attagcgtca aaaagcccgg
     1141 tgcctggcgg cggcggagtg tccgccctgg taggggccat aggcacggca ctggccagca
     1201 tggtatgtaa cctgacaacc gggaaaaaga agtacgccca atttgaagat gacatacaga
     1261 gaatcttaaa aagagctgca gagttgcaag atgacctgat gaagatgatc gatgaggacg
     1321 cagaaaactt tctccccctt tcaaaagcct acggcatgcc aaaagatacg gaagaagagc
     1381 ggcgattaaa agaggaaact ctggaaaagg ctctaaaaca ggcctgcgaa gtgcccgtaa
     1441 aaatcgtaaa ggcatgttat gaggcgatca agttacatgc tgaattggta gacaaaggtt
     1501 cgagacttgc cataagcgat gtgggtgttg gagttcagtg cctaagagct gccataatca
     1561 gtggttggct aaatatcgta ataaatatca actcaatcaa agaccaggat tatgtaaata
     1621 aggtcaggga agaaaccgag aacctggtaa gacaaggtgc aatgcttgct gacaaagtat
     1681 acgagcaagt ggaaggcatt ttaaacaaat aagcttcagg ggacctgaca gggggacgtt
     1741 tcgcttgcca ggttggggac ctgacagggg gacgtttcgt ttgccaggtt tgtaaggcag
     1801 cttgtcgcat gcagaatggc aagccaaaaa gtccccttgt cttcctgccg tccccgggtc
     1861 tttagcttgc tagaaattga aataagggag gatttcaatg ggaatattga tcaaaggtaa
     1921 gccggtggcg gataagatta aggaaaaact aaagaaagaa gttgctgagc tcaaggaaaa
     1981 gggtattact ccaaagcttg ccattatcag ggtgggagat gaccccggcg acatggcata
     2041 cgaacgcggg gccattaaga caatggtaag cgtaaatata gagacggatg ttagggacct
     2101 gtctaaagac atcagtcaag acgaatttat aaaggaattg aaaagtatca acgatgacaa
     2161 atccgttcat ggaatcctga ttttaaggcc attgccggac cagcttgatg aaaatgtaat
     2221 caaaaatgtg attgcaccgg aaaaagatgt ggactgcttt agcccggtaa atgtggccaa
     2281 agtctttgaa ggtgacgaaa ctggctttcc accatgcact ccttctgcag tgatggaaat
     2341 attaaagcat tatgaaattc ccttgaaggg caaaaacgcc gtagtcatcg gaagatcgat
     2401 gatagtggga aaaccggcgg ccatgctgct tttaaaagaa catgccacgg tgaccatatg
     2461 ccattccagg acaaaagacc tgcctaaggt tgcatcgggc gcggaactgc tggtagtggg
     2521 cataggaagg gcgaaaatga tcgactccag ctacattaga gaaggggcag ttgtcattga
     2581 tgtaggcatt aatgtagacg aagatggaaa gttgtgcggc gatgtggaca ccgatgattg
     2641 tgtggataag gcttccatga ttactccggt tcccggagga gtgggttcgg tcacaacttc
     2701 ggttttggca aagcacgtgg ttaaagcatg taagatgcag caagctttat aaaggctaaa
     2761 ggagggaaca agccttgatt atttcccagc ataagccctt tgaagagatt gaagagctgc
     2821 tcaggggctg tgaaaagctt gtacttgtcg gttgcggtca atgcgctact gcatgcatga
     2881 gcggtggcga aaaagagctt atggaaatga aagaagaatt agagggcata gacaaaaaga
     2941 ttttaactta catagtgccg gaaaccagtt gcaattacct cctggttcga agagacttga
     3001 aaaagataaa gaatgcattg gaggaagcgg atgcggtttt gtcttttgcc tgcggtgacg
     3061 gcacccagac cattgcaaaa cttgtaaata tcccggtcta tcccggtaac aataccctgt
     3121 ttgtggggga agtggaaagg gtcgggctgt acagcgaggc atgcaggacc tgcggagatt
     3181 gtgttttggg ggtgaccggt ggaatatgtc cggtaaccag atgtgccaaa tccctgctga
     3241 acggtccttg cggcggtgct aaagacggga aatgtgaagt taatcctgaa aatgactgtg
     3301 catggatctt aatatataat aagttgaaag aattaggcca actggacaaa ttgactgaaa
     3361 ttcacgaacc gaaggactat tcccggcaag catacccgag gaatcttaat ctccgaaatc
     3421 aggacggagg tggaagatga tgagtcgatt gcggaaggcg ctggagagcg gcgaatttgc
     3481 tgtcaccgcg gaattggcac cccctaaagg gactgatttt tcccacgcac tgcaatgtgc
     3541 agagctattc ggtgacaggg ttgatgccat aaatgtcacg gactttcaat catcgtccct
     3601 gaaggcaaca tcactggcca tgtgtaaggc tttaattgat atgggttttg agccggttct
     3661 acaaatcacc ggacgagaca gaaatcgatt agccatacag ggggaaatgc tgtcagccgg
     3721 ctttttcggg gtaaaaaatc tattggccct taccggagac cataccaccg ttggtgataa
     3781 tcccggtgca aaaccggtgt ttgacctgga tagtgtgggg atactgcagg cggccacaat
     3841 gctggccaac ggtgttgata tgagcggaaa caaactggag ggaagtcctt ccttctattt
     3901 gggagccacc gtcaccccag aatacgaccc tatagagcta cagctcgtta aaatggagaa
     3961 aaaaataaaa gccggagcta agtttttcca aacccaagca gtatacgatc tttccacaat
     4021 ggaaaagttc aaggaaatga cgagacactt aaatacgaaa atactagtgg gaatcattcc
     4081 cttaaaatca gcaggtatgg caaggttcat gaacaaaaat attccgggga taaatgttcc
     4141 cgagcatttg atcgacaggc tgaaaaatag cggggatccg gtcaaagaag gaataaaaat
     4201 tgccggtgaa ctcatagtgg agctgagaga caagaatttg tgcgacggcg ttcacataat
     4261 ggccattggt gctgaagaaa atgtacctct aattttggac gaggcaggac tataaaaagg
     4321 ggaggcattt tcaaatgaaa gtagtggtga taggagcggg tcccggcgga tacgaggcgg
     4381 ccataaaggc ggccaaactt ggggcgaaag taaccgttgt ggaaaaagac aacctgggtg
     4441 gaacctgctt aaatcgcggc tgcataccga caaaagccct gctggcatct tcggatgttt
     4501 ttacgacggt taagaacgcc accaaattcg gcgtgaacgt tatgggagaa gtgactgctg
     4561 actttcctgc gatgataagc aggaaggaca aattggtgtc acaactgatc aaaggcatag
     4621 aattcctatt taaaaagaac ggcattgagg tcgtttacgg tactggaaaa ctgatagaca
     4681 aaaacaagtt tgaagttaca cgagatgatg gttcaaagga gatactggaa gctgacagaa
     4741 taattctcgc aacaggttcg gttccggtct gtcccccatt attcaattat gatgggaaaa
     4801 atgtaattac aagtgatgaa gtgctaagcc tcaacaaggc acccgcttca attattatag
     4861 taggcggcgg cgtaattggc tgcgaaattg gccagttttt gaggcgcttg ggcaccgagg
     4921 taaccatagt agaaatgatg ccccacatac ttcccatgga agacgaagat gttgcaaacc
     4981 agctgctgcg gcagtttaag agggataaaa tcaaagtgat tgctggcaaa gccatcactt
     5041 ctgtgaaagt agaagaaaac agggtagttg caggcttaga agatggagag cttgaagcag
     5101 aaataatgat ggtttccatt gggagaaaac cttacaccga tggattgggc ttggaaaacg
     5161 taggaattca aactgataaa gccggcagaa ttccagtcaa caaaaaaatg gaaaccctag
     5221 ttgaaggaat ttatgccatc ggagatattg tggattcgcc atttttagcc catgtggcat
     5281 caaaagaagg cctgattgcc gctgaaaatg ccttgggtgg aa
//
LOCUS       DAONUO010000129         5309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11357_length_5309_cov_4.147887, whole genome shotgun sequence.
ACCESSION   DAONUO010000129 DAONUO010000000
VERSION     DAONUO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11357_length_5309_cov_4.147887"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK768_05785"
     CDS             <1..275
                     /locus_tag="PK768_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQA60515.1"
                     /translation="MDGFGAIISFEVEGGLDRAKQVVNSVKLAKLAVSLGDCETLIEL
                     PAAMTHAGYPKERLREFGLSESMIRLSVGIENVEDIIEDLNNALNA"
     gene            325..1110
                     /locus_tag="PK768_05790"
     CDS             325..1110
                     /locus_tag="PK768_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HQA60516.1"
                     /translation="MAKYGIFSWFGFVMPLSERLELIKKAGFDSTCLWWEDEEGAYPV
                     KKTDMPKMVRDFGLSLDNIHTPFCNSNDLWSESLSARSEIVKQYLGWLEDCARFDIPI
                     MVMHIMEGDILPELSSNGIESMSCLVKKAEELRVKIAVENTKFADGVDFILSEIESEY
                     LGLCYDSSHGRLHGCEFLLKDFGRRLITCHISDNDGEMDRHWLPGNGIIDWKEFCESF
                     PKKHYLGNLMLEVYPTGDEIKGGPAEFLNKAFKSISALGSFSS"
     gene            1146..1835
                     /locus_tag="PK768_05795"
     CDS             1146..1835
                     /locus_tag="PK768_05795"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HQA60517.1"
                     /translation="MFPDWKNWRHVVKLDPDKAADEYMIEQVVASGTDAIIVGGTQNI
                     SREKVLRLYKLLKPYPLPKILEVSSVDAISFGFDGYLIPIVLNAGHPQWLIGAHKEAV
                     KLIGELMDWQVLLPEGYIILNPDCAAAELTKAINPLSREDVLAYARCGENLFSLPIIY
                     IEYSGKFGNPELVSYIKNRLNNARVFYGGGIDNAQKAEMMGKVADTIVVGNMVYTEAI
                     KNLPDTVKAVS"
     gene            2049..3278
                     /locus_tag="PK768_05800"
     CDS             2049..3278
                     /locus_tag="PK768_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQA60518.1"
                     /translation="MKGRKKLIIFTGVLLAVVAFFIFRVKFQPNPVMALPTVSAVKGD
                     ISSKITALGRVETVQEQEVYSPIQGTVEFVVEEGQQVKSGDVILKIDDEELRLELAQV
                     QSKVKQQRLELSRLLDGTRFEELEKARIKYKDALTAYEAALDDYEKNQELFNLGAISE
                     KDLLNIKREYDAKKNQLSIMELDLKLLENPDEKEIALRQIALEEAENNLANIEKKLSK
                     TVVYAEFDGIVLEQNIKPGMMVTPGTLLLRVGDLSDLQIEISVNEYDAAKLRLGQKAI
                     ISGQGFGDRTYDGEVVKIAPSASVTQTSRGNESVVKAAVRVIEHDGHIKPGFSATVEI
                     TVDEKQDAILIPLECVIEEEGSKQVMVVKDGNISKREVRTGLENDLYVEIVQGLSEGD
                     EVLQDPSQGSDVTGDAL"
     gene            3293..3958
                     /locus_tag="PK768_05805"
     CDS             3293..3958
                     /locus_tag="PK768_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60519.1"
                     /translation="MTKVYKSGENEVVALKDVNLHIKKGEFVAIMGPSGSGKSTLMNI
                     LGCLDKPTSGEYYLEGKAISQLDSRQLAHIRNKKIGFVFQNFNLLPRMNALRNVEIPM
                     IYAGVSSGDRRARAMEALKSVGLEDRWHHKPNELSGGQRQRVAIARALVNSPRIILAD
                     EPTGNLDSKSGAEIMEIFTELHRNGATIVLVTHENEIAKYAQRIVFFRDGRKVDEKSG
                     ELL"
     gene            3933..5135
                     /locus_tag="PK768_05810"
     CDS             3933..5135
                     /locus_tag="PK768_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60520.1"
                     /translation="MRSPVNFFESIIIAWESIVANKLRSLLTMLGIIIGVSSVITVSA
                     LGQGGRAAINKEMDAFGANRFGIYYKYNPDNPITDADVFTLQDVLAIKEISPAVESIA
                     PISWDTTSIRSKGKEIGVEIAATTADYAKIANLKLDAGRFFSNEDENAKRFVAIISES
                     LAKELFGSSTAIGKKIMVSNRPFNIIGVLAREESIFSMGQDSKLMYIPISTYFSYSGT
                     NWINGIQGKAVSQNEVDRAIEHSLSILHRRHRNEDKYDAWNSQKEMDSVNQVTGIVTL
                     VISAIAGISLLVGGIGIMNIMLVSVTERTREIGIRKALGAQEIDIMIQFLIESLVLSF
                     SGGVLGMFIGFGVSKIIALFIKIPTFISWQMVAIAFTFSAGVGICFGLYPARKAAYQD
                     PIEALRYE"
BASE COUNT         1662 a          945 c         1319 g         1383 t
ORIGIN      
        1 agatggacgg cttcggagcc ataataagct ttgaagtgga aggtggcctt gacagagcaa
       61 aacaagtggt aaatagcgta aagctggcca agcttgccgt gagcttagga gactgcgaga
      121 ccttaattga acttcctgca gccatgaccc atgccgggta ccccaaagaa aggctgcgtg
      181 aattcggact ttcagaaagc atgataaggc tttccgtggg cattgaaaat gtagaagata
      241 ttatagaaga cttgaataat gcccttaatg cttagttaat gagaaaaaat atgtcttatt
      301 aatgcaataa aggagaacaa gcttatggcc aaatacggaa tattttcctg gtttggattc
      361 gtaatgcctt tatccgagag gctcgaactg ataaaaaagg ccggctttga ttcaacctgc
      421 ctctggtggg aagatgagga gggagcttat cccgtcaaaa agaccgacat gcctaaaatg
      481 gtccgggatt ttggcttgag cttagacaat atccatacgc ccttttgcaa ttccaatgat
      541 ttgtggagtg agtccctttc ggccaggtct gaaatcgtaa aacagtattt aggctggctt
      601 gaggattgtg ccagattcga tattcccata atggtcatgc acatcatgga aggagatatc
      661 cttcccgaac taagcagcaa cggaatcgaa agcatgtcgt gcctcgtgaa aaaagcagaa
      721 gaattgaggg taaagatagc agtggagaac accaaatttg ccgacggcgt tgattttatt
      781 ttatcggaaa tagagtcaga gtatttaggg ctttgttatg atagttctca tggcagattg
      841 catggatgcg agttcttgtt aaaagatttc ggccggaggc ttataacctg ccatatttca
      901 gacaatgacg gtgaaatgga caggcactgg ttgcccggaa atgggataat tgattggaaa
      961 gaattctgcg aatcattccc gaagaagcac tatttgggaa atctgatgct ggaagtatat
     1021 ccgactgggg atgaaataaa gggcgggcca gcggaatttt tgaacaaagc attcaagagt
     1081 atttcggctt tgggaagttt tagctcataa ttatattctc aataatacga aatggaggct
     1141 aatctatgtt cccggattgg aaaaactggc ggcacgtggt gaaacttgat ccggacaaag
     1201 ccgcagatga atatatgata gagcaggttg tagcaagcgg caccgatgcc atcatagtgg
     1261 ggggcacaca aaatattagc cgggaaaaag tgctaaggct ttataaactc ttaaagccat
     1321 atcccctgcc caaaatcctg gaagtatcct ctgtcgacgc tattagcttt ggctttgatg
     1381 gctatctaat accgatcgtt ttaaatgcag gccatcccca atggctcatc ggagcgcaca
     1441 aggaggcggt gaagctcatt ggagaattga tggattggca ggtcctttta cctgaaggct
     1501 acataatcct aaacccggat tgtgctgctg cagagctcac aaaggccata aatcctttaa
     1561 gcagggaaga tgttttggca tatgcccgat gcggtgaaaa tctcttctcc cttccgataa
     1621 tttacataga atacagcgga aaattcggaa acccggaact cgtatcatac ataaaaaata
     1681 gattgaacaa tgccagagtg ttttatggcg gaggcatcga taatgcccaa aaagcggaga
     1741 tgatgggcaa ggttgcggat acaattgttg tcggaaacat ggtctatacg gaggccatca
     1801 aaaacttgcc ggataccgtt aaggccgtga gctagcttac aaatttaaga aagatttaag
     1861 agttctttaa gaaaggttta atcgaatttt atgctttatt gtatttaccg taattagcat
     1921 aggtgttata atggtgaaaa aaataacaat tacaaaaagg acataaaaaa taatctactc
     1981 tgacttttta agcttttatt tcatataata gtgcacactg gatgtctttg aataaaaggg
     2041 ggattttggt gaagggtcgg aaaaaactta taatatttac aggagtttta ctggcagttg
     2101 ttgctttttt tattttcagg gtaaaatttc agcctaatcc agttatggct ttgccgactg
     2161 tcagtgccgt taaaggtgac atctcctcta agattactgc ccttggaagg gtggaaacgg
     2221 ttcaagaaca ggaggtttat tctcctattc aaggtacagt agaatttgtg gttgaagaag
     2281 gacaacaggt caaaagtggg gatgtaattt taaaaatcga tgatgaggaa ttaaggctgg
     2341 agctggcaca agttcaaagc aaggtgaaac aacaaaggct tgaactttca aggctgctgg
     2401 atggaacaag atttgaagaa ttggaaaaag ccaggataaa atataaggat gcactgactg
     2461 cttatgaagc tgccttggat gattacgaaa aaaaccagga actttttaat ttaggggcaa
     2521 tatcggaaaa agacttgtta aatattaaaa gagaatacga tgctaaaaaa aatcagctgt
     2581 cgattatgga attggatctc aagttattag aaaatcctga cgaaaaggaa atcgcattaa
     2641 gacaaattgc gctggaagaa gcggaaaaca atctggccaa cattgaaaag aagttaagta
     2701 agacagtcgt ctatgccgaa tttgatggaa tagtattgga gcaaaatata aagcccggaa
     2761 tgatggtgac gccaggaact ttactgctta gagtgggcga cctttcagat ttacaaatag
     2821 aaatcagcgt taatgaatac gatgcagcca agttacgact cggacaaaaa gccattattt
     2881 ccggtcaagg ttttggcgac aggacatatg atggtgaagt tgtgaaaatt gccccttcgg
     2941 cctcggtgac acaaaccagc agaggcaatg aaagcgttgt aaaagcagct gtaagagtaa
     3001 tcgagcacga tggccatata aagccgggtt tttcagccac agtagagata acagtagatg
     3061 aaaaacaaga tgcgatttta atacccctgg aatgtgtgat agaggaggaa ggcagcaagc
     3121 aggtaatggt agtaaaagac ggaaatatat ctaaaaggga agttagaaca ggcctagaaa
     3181 atgacctgta cgtagagata gtacaaggcc tgtccgaagg tgatgaggtc cttcaggacc
     3241 ctagtcaagg cagcgatgta acaggtgatg ccctgtgata caggttgata acatgacaaa
     3301 agtatacaaa agtggcgaaa atgaagttgt ggcacttaaa gacgtaaatc tccacataaa
     3361 aaagggcgag ttcgttgcca taatgggccc atcgggttca gggaaatcta cccttatgaa
     3421 tatactcggt tgcctcgaca agcccacgtc gggagagtat tacttggaag gaaaagccat
     3481 atcccagctt gatagccgcc agctggcgca tatcagaaac aaaaagattg gatttgtctt
     3541 tcaaaatttt aatttacttc ctcgaatgaa cgcccttaga aacgtagaaa tacccatgat
     3601 ttatgctgga gtctcatctg gggataggcg agcaagggca atggaagctc tgaaatccgt
     3661 tggactggaa gataggtggc atcataaacc aaacgagctt tccggcggtc aaaggcagag
     3721 agttgctatc gcccgagcct tggttaactc tccaaggatc atactggcgg acgaacctac
     3781 gggcaatctg gatagcaagt ccggagctga gatcatggag atttttactg aactgcaccg
     3841 aaatggagcg acaatcgtct tggtaactca tgagaatgaa atcgccaagt atgcgcaaag
     3901 aatagtgttt ttccgcgatg gtagaaaggt ggatgagaag tccggtgaac ttctttgaga
     3961 gtataataat tgcttgggag agcattgttg caaataaatt gcgctccctc ctcacaatgc
     4021 tgggcatcat cattggggtt tcttcggtaa taacggtttc ggccttgggc caggggggaa
     4081 gggctgcaat caacaaagaa atggatgctt ttggggccaa tcgatttggc atctattata
     4141 agtacaatcc cgataatccc ataaccgatg ccgatgtctt cactcttcag gatgtgcttg
     4201 ctataaaaga aatttcccca gcagttgaga gtattgcacc tatcagctgg gataccacaa
     4261 gcatcaggtc aaaggggaaa gagattggag ttgaaattgc ggctacaact gcggattatg
     4321 ccaagatagc aaatcttaaa ttggatgcag gcaggttttt tagcaacgag gatgagaatg
     4381 caaaaagatt tgtagcgatt ataagtgagt cactggcgaa agaactgttc ggttcctcta
     4441 cggcaattgg aaaaaagata atggtgagca atcggccttt taatataata ggggtactgg
     4501 cgcgggaaga gtctattttt tccatgggcc aggatagcaa gttgatgtac ataccgattt
     4561 caacgtattt ttcctacagt ggaaccaatt ggataaatgg cattcagggc aaggcggtat
     4621 cccaaaatga agtagataga gccattgagc attcactaag catcctgcat cggcgtcata
     4681 gaaatgagga taagtacgat gcctggaatt ctcaaaagga gatggacagc gtaaatcaag
     4741 ttacagggat tgtaactctt gtaatcagtg ctatagcagg catctcctta ctggtgggtg
     4801 gaataggcat tatgaatatc atgctggtat cggttacgga gcggaccagg gaaatcggca
     4861 tccggaaggc attaggcgct caagaaatag acattatgat tcaattttta attgagtctt
     4921 tagtgctctc tttttccggt ggcgtcttgg ggatgttcat aggatttggc gtatctaaga
     4981 taatagcctt gtttattaaa atacccacct ttatatcatg gcagatggta gcaatagctt
     5041 ttactttttc ggcaggggtt ggtatctgtt tcggcttata tcccgcacga aaggcggcct
     5101 accaggatcc catagaggca cttagatatg aataaggcag cttggctgct ttttttgttt
     5161 taagacatat tccctcttta tgcaacatat gttttaaagt gtaagcagca atggtggagg
     5221 gaatatttat gagcgtgttt cataccgatc ccaatgtagg tttgtctact agggaaattc
     5281 cccttaaaaa gaaactcttt ggagacaat
//
LOCUS       DAONUO010000130         5255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11498_length_5255_cov_3.631731, whole genome shotgun sequence.
ACCESSION   DAONUO010000130 DAONUO010000000
VERSION     DAONUO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11498_length_5255_cov_3.631731"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK768_05815"
     CDS             <1..255
                     /locus_tag="PK768_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HQA60521.1"
                     /translation="SGVLNFTVETMKNTIDAIKEAGLKDNRLIILGGNHLTEEACEYT
                     GADFCTNDASIGVKFCKEWINKKHQIGSINHGIADISSNC"
     gene            286..945
                     /locus_tag="PK768_05820"
     CDS             286..945
                     /locus_tag="PK768_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQA60522.1"
                     /translation="MKPGDALYSENTLCKTYNASRMTVRKGLAILAGEGYIYSIPGKG
                     NFVKEPDSTTYTLYYDEKNNLINSVDKTKLLEVNIIMPTSKLIEALQITKNKKVIMIR
                     RLFYTDGEPVAYDLKYLPYYKGMPIVEKEIRYATFPEIVSKKTSLFAIKKELIISAQM
                     PDEEIRRHLNIYNELPLMVVEQKLYDIEDKPIGLGITYFRSDYCKLYAQASFRDKTSH
                     E"
     gene            1059..3140
                     /locus_tag="PK768_05825"
     CDS             1059..3140
                     /locus_tag="PK768_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60523.1"
                     /translation="MSCGRIKRITTIFLTILVILSISTAQVCAASLQFTDISASRYDW
                     VRPYIQKMSLAGVVKGMTDTTYGPDANVTREQLVTMLVRLMGWESLTSGKSLPSNFPR
                     ASSVAPWARPYVAVAIEKGIVSGKDFDDFRPSDAAKRSEVAVFAVKALGLGQEAESRK
                     TLSIIFGFTDVHMIELETRPYVELAVEKGIMKGFPDNSFKPDEKFTRAQMAVLLHNLS
                     KLTKVQNIISGVVQDVETDILPNVEVRLDDGTLGTYTVNISSTLIYKEDESGNLTKSE
                     LKDIKAGDRISIIASGTSAQYIDVGYGSTAPVTDGYTVEGTIKEIDLIRSTLTVRTSD
                     NRERTYNIKNQTKVYIDGTSATIYQLASGQSVKLTVSETDVERIDVKGEDKVVKGVIR
                     AVNRVTNVLTIENEKTDEYESYSIASNVKIYKDNRAADIYSLTIGDVATITVSGSRIV
                     EIDAESATRDITGILMGIEYAKNPVLVVELDDGRENKYELDKDVTIRKNGKRAELSDL
                     KKGDEVTLTLEYDLVTRVAAESVKRDIKGTVKAITFADKITVTVIDDKGKEHDITITS
                     DTKITKDRKRIDATEIRRDYYLDMEVENDEAISIDVTVKDTHDVLRGTVIGIHENIEV
                     IVISAKTEDGTREISIRYTSDTVVLKSSRDGARSHRITRIEEGNEIVAIGSYEGGLFV
                     AVTIQDITFSD"
     gene            3458..3832
                     /locus_tag="PK768_05830"
     CDS             3458..3832
                     /locus_tag="PK768_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQA60524.1"
                     /translation="MKRNNVLIVDDQHWVKTMLKEALSYSGFDAHVASDHVEAIEIVK
                     VEQPEIVIIDVNLPEIDGFRLLSIIRSIKPDVKAVFISGSSNVNVARRAMAEGALRYF
                     IKPFDVFDFISYLTEISQLDTA"
     gene            3847..4800
                     /locus_tag="PK768_05835"
     CDS             3847..4800
                     /locus_tag="PK768_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA60525.1"
                     /translation="MASERRQRSEEFGKMIGNFQKYFTKSSCPLKQGLPLYLSEGCHL
                     VLIEYQAEGNKTFEYEVDFFILEDEKGILGYLVSYEDTENLKELERKNMQLESLLAEK
                     EIAASYVHELKNPLFSIRGFLQIIRQSLSDGDKRKEYADIAINELDRMNNLLNDYLSR
                     YRAHNTPEIKSKGLSVKKIIEELIVFFEHSLHLKGITYKIEFEDSDLQVLMGKEQLMQ
                     VLLNIVQNSIEAMDKGAHLSIRAFKDEDWVCIEIKDEGVGIKQSDLDKIFTPFFSTKE
                     NGTGLGLYITKRIVDSNGGSINIKSTEGKGTTTYLKFPRAY"
     gene            5095..>5255
                     /locus_tag="PK768_05840"
     CDS             5095..>5255
                     /locus_tag="PK768_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HQA60526.1"
                     /translation="MLITELEKKTIAQLHEIAKEKKIPGYYKYRKRELILKLIEAFSE
                     EGEGMAEGV"
BASE COUNT         1775 a          900 c         1223 g         1357 t
ORIGIN      
        1 agcggagtcc tgaactttac cgtggagacg atgaagaata cgatagatgc catcaaggaa
       61 gcaggattga aagacaacag actgattatt ttggggggaa atcaccttac agaggaagcg
      121 tgtgaatata ccggtgccga tttttgcact aacgacgcat cgattggagt aaagttttgt
      181 aaagaatgga ttaacaaaaa gcatcaaata gggagtataa atcatgggat tgccgatata
      241 tcttcaaatt gttgaggaca taaaagaaaa aattaattcg gagagttgaa accgggtgat
      301 gctttgtatt cggaaaacac cctatgcaag acgtacaatg ccagccgcat gacggtgagg
      361 aaagggcttg ctattttggc cggcgaaggg tacatttatt ccattccggg caagggaaac
      421 tttgtgaaag aacctgacag cactacatat acgctttatt atgacgaaaa gaacaaccta
      481 ataaatagtg ttgataagac aaaattgctg gaggttaata ttattatgcc taccagtaaa
      541 ttgattgaag ccctgcagat aactaagaat aaaaaggtta tcatgattag aagattgttt
      601 tacaccgatg gagaaccggt tgcctatgac ctgaagtacc tgccttatta taagggaatg
      661 cccattgtag aaaaggaaat acgctatgcc acattccctg aaatagtgtc caagaagacc
      721 tcgctttttg ctataaaaaa ggaactgatc atatcagccc aaatgcccga tgaagagatc
      781 aggagacacc tgaacatcta taatgagctt cctttgatgg tcgtagaaca aaaactgtat
      841 gacattgaag ataagcccat aggattaggt attacctact ttaggagtga ttattgtaag
      901 ttatatgcac aagcatcatt tagggacaag acaagtcatg aatgaagtct ttgacctttg
      961 cactgatttg gcaaaggtat tttttatttt aaaaagagga ctaagccctg cttttgtaga
     1021 atattaaagc atattatgtt tcgacggagg gagattttat gagctgtggg agaataaaaa
     1081 gaataacaac tatatttttg actatactag tcattttaag tataagtact gcacaggttt
     1141 gtgctgcaag tttgcagttt actgatatta gcgcatcgag atatgactgg gtcaggccgt
     1201 acatacagaa gatgagttta gctggagttg taaagggcat gaccgacacc acttacggtc
     1261 ctgatgccaa tgtcaccagg gaacaacttg taaccatgct ggtgaggctc atgggatggg
     1321 aaagtctgac cagcggcaaa agcctgcctt ccaattttcc cagggcgagt tcggtagcac
     1381 catgggcgag accttatgta gctgttgcta ttgaaaaggg gatagtgtcc ggaaaggatt
     1441 ttgatgattt cagaccctcg gatgcagcca aaaggtcgga agtagcagtt tttgcggtaa
     1501 aggccttggg ccttggccag gaagcggaaa gccgcaaaac tttaagtatt atctttggat
     1561 ttactgacgt ccacatgatt gagctcgaaa cgaggccgta cgtggaactt gccgttgaaa
     1621 agggcatcat gaaaggtttt cccgacaaca gcttcaagcc cgacgagaag tttacccgtg
     1681 cccaaatggc agttttgctc cacaatctgt cgaaacttac aaaagtacaa aacattatta
     1741 gcggtgtggt gcaggatgta gaaactgata ttctgccgaa tgtcgaagta aggcttgacg
     1801 atggcacatt ggggacgtac acggtaaata taagcagcac tttaatttac aaagaagatg
     1861 aaagtggaaa tttaacaaaa tccgagttaa aggatataaa agcgggggat cgaataagca
     1921 taattgccag tggaacttcg gcccaatata tagatgttgg ttacggcagc acagctcccg
     1981 taacggacgg ttacacagta gaaggcacta ttaaagaaat tgacctgatt agaagtacct
     2041 tgacggttag gacaagtgac aacagggaaa ggacctacaa tattaaaaac cagaccaagg
     2101 tgtatataga cggcacttcg gccactattt accagttggc ctcaggccaa tcggtaaaac
     2161 taaccgtttc cgaaaccgat gtggagcgaa ttgatgtcaa aggtgaagac aaggtagtta
     2221 aaggcgtaat ccgagcagta aatagggtta ccaatgtgct cacaatcgag aatgaaaaaa
     2281 ctgatgaata tgaaagctat agcattgctt ctaatgtaaa aatatataaa gataataggg
     2341 ctgctgacat ttacagcctg acaataggcg atgtggccac catcaccgta tccggcagca
     2401 ggatagtgga aatagacgcg gaaagtgcga caagggacat taccggcata ttgatgggta
     2461 ttgaatatgc caaaaatccg gtgctcgtag tagaactgga tgatggcagg gaaaacaagt
     2521 atgaacttga caaagatgtt accattagaa agaatggcaa gagggcagag ctaagcgacc
     2581 ttaaaaaggg tgatgaagtg accctcaccc tggaatacga tttggtgaca agagttgctg
     2641 ctgaaagcgt caagcgtgac ataaagggca ccgtaaaggc cataaccttt gcggataaaa
     2701 tcaccgttac agtaattgac gataagggca aggagcatga tattacaatt acttcagata
     2761 ctaaaattac caaagacaga aagcgcatag atgccacgga aatccggcgg gattattacc
     2821 tggacatgga agtggaaaac gatgaagcca taagcattga tgtgacggtg aaagacaccc
     2881 atgacgtgct aaggggcacg gtaatcggta tacacgaaaa cattgaggtc atcgtaatca
     2941 gtgccaaaac ggaagacggc acgagggaaa tatccatccg atataccagc gacaccgtgg
     3001 tactcaaaag cagcagggac ggtgccagga gtcaccgcat caccaggatt gaggaaggca
     3061 atgaaatcgt ggccatcggc agttacgaag gaggactgtt tgttgcagta acaatacagg
     3121 acattacctt ttctgattaa ccgggtataa catagaatga ttagcattaa cttgtctttt
     3181 atggccttgc cttgggcaag gcctaaactt atatttatag tgttttatct ttgccttcag
     3241 ccttcgaagt ctgtaatata gccatggaaa gacatcagaa cttttacaga ctattataaa
     3301 tatatatttt tttagcgatg ctcaaataat tcatgcgata taaaaataaa aggaggaatt
     3361 tatcaacaaa tatagaatat aatagtgaat gccgggaagt acgtcctttg attattgtcc
     3421 atggccacaa gttcttatga tttggtaggg gaatatcatg aaaaggaaca acgtccttat
     3481 tgttgatgac caacattggg taaaaacaat gctgaaggaa gcactgagct attcaggctt
     3541 tgatgctcat gtagcgtcgg accatgtaga ggcgatagaa atagttaaag ttgaacagcc
     3601 ggaaattgta atcattgatg taaatttacc agagattgac ggatttcgcc ttttatcaat
     3661 aatcaggagt ataaagcccg atgttaaagc cgtgtttata tctggttctt ccaatgtgaa
     3721 tgttgccaga agagctatgg ctgaaggagc cctaagatat tttattaaac cctttgatgt
     3781 ttttgatttt ataagctact taactgaaat ctcgcaatta gatacagctt agagaaggga
     3841 agtttaatgg catctgagag gaggcaaaga agtgaagagt tcgggaaaat gattggcaat
     3901 tttcagaaat acttcacaaa atcatcttgc cccttgaagc aaggtctgcc gttatatctt
     3961 agtgagggtt gtcatctggt gttgatcgag tatcaggcag agggaaacaa gactttcgaa
     4021 tatgaggtgg attttttcat ccttgaagat gaaaaaggga ttttaggata tttggtctcc
     4081 tatgaagata ctgaaaatct taaagaactt gaaaggaaaa acatgcagct tgaaagtttg
     4141 cttgcggaga aagagattgc tgccagttat gtacatgaat tgaaaaatcc cttattttcc
     4201 attcgaggtt tcctccagat cataagacaa agcctctcgg acggtgacaa aagaaaggaa
     4261 tatgcagata tagccataaa cgaattggac cgcatgaaca atctgctaaa tgactatcta
     4321 tccagataca gagcccataa cacacctgaa ataaagagca aaggcctatc ggtaaagaag
     4381 ataatcgagg aattaattgt tttttttgaa cacagtttgc atctcaaagg tataacatat
     4441 aagattgaat ttgaggatag tgacctgcag gttttgatgg ggaaagaaca gttaatgcaa
     4501 gtgctgctca atatcgtaca aaactcaatt gaagctatgg acaaaggggc tcatttaagt
     4561 ataagggcct tcaaagacga ggactgggta tgcattgaga taaaagatga aggggtggga
     4621 ataaagcaga gcgatctgga taaaatattt accccatttt ttagcacaaa agaaaacggg
     4681 acaggcctgg gtttatacat tactaagcga atagttgaca gcaacggggg cagcataaat
     4741 ataaaatcaa cggagggaaa agggaccacc acatatttga agtttcccag ggcatattaa
     4801 aaataacata gtgtcaatca aaatattgac ggtaaagttt ataaatggta taataactaa
     4861 caaaaggcaa atcctcctcg gtgtatctat aaaaaagggg gccctgttag ggctcttttt
     4921 tttaccttgc ctactgcttt ccattgaata tttttcacat cgatgaacat aattttaaag
     4981 aataaatttc gtataattct tgtcagcgga agcttttctg tctttcacat ttccattagt
     5041 acatcgtgtt ttcaaaaaaa ttcttttata atttaataat aaggaagtga aatcttgtta
     5101 attacagaat tagagaaaaa gaccatagcg caactgcatg aaattgcaaa agaaaagaaa
     5161 attcccggtt attataagta ccgcaagcgt gaacttatcc tcaaattaat agaagccttc
     5221 agcgaagagg gcgaaggtat ggcggaaggt gtttt
//
LOCUS       DAONUO010000131         5248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11523_length_5248_cov_2.901983, whole genome shotgun sequence.
ACCESSION   DAONUO010000131 DAONUO010000000
VERSION     DAONUO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11523_length_5248_cov_2.901983"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="tgt"
                     /locus_tag="PK768_05845"
     CDS             complement(<1..771)
                     /gene="tgt"
                     /locus_tag="PK768_05845"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQA60527.1"
                     /translation="MKFEVLKQSTNTSARLGFIETPHNSFKTPVFMPVGTQATVKTMT
                     PEELEQIGAKIILSNTYHLYLRPGHELVKEAGGLHKFMNWGGSILTDSGGFQVFSLGD
                     LREIKEEGVTFRSHIDGSLHFIGPETSIEIQNALGSDIIMAFDECIPYPATYDYVLDS
                     VERTTRWAERCLKAHRNPDKQTLFGIVQGGMYKDLRRKSARDITSLGFNGYAIGGLSV
                     GEPKELMYEILEYTVPFLPYDKPRYLMGVGTPEDLFEGV"
     gene            complement(768..1403)
                     /locus_tag="PK768_05850"
     CDS             complement(768..1403)
                     /locus_tag="PK768_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HQA60528.1"
                     /translation="MKLSSRIAKIVIILELILASLLAVGVIISFTDILGYFRILYYTS
                     PIDTLPVMQTFLGHILTLVIGLELAVMLIRHTPSSVIEVLLYAIARKMIIESKTMTDI
                     LLGVVAIGVLFFINKFFDPARNFITEVNIVNPATLVKDINKKLDVNIPEDLGNTVGGV
                     VSKLSQESREKLERGKTFRVADAEITILSMDGELIRELKVVKAEEKDDILL"
     gene            complement(1419..2444)
                     /gene="queA"
                     /locus_tag="PK768_05855"
     CDS             complement(1419..2444)
                     /gene="queA"
                     /locus_tag="PK768_05855"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQA60529.1"
                     /translation="MKVDEFDFYLPEELIAQEPIENRSKSRLLVLNKNNGTIEHKSFE
                     DIIHYLQPGDCLVLNDTKVIPARLYGRRKHTLGKIEMVLLKEVEADTWEVLVKPGRRA
                     RTGAEIVFGDGDLTAQVIGTTNYGGRIVKFFFKRPFQTLLNELGEMPTPPYIKKKLQE
                     KSRYQTVYAKKPGSAAAPTAGLHFTEELLDNIRQKGISTAYITLHVGLGTFRPVTVEN
                     IEEHKMHAEYYEIGQDAADVINSTKESGGRVIAVGTTSTRTLETSADLNGKIKPCSGW
                     SDIFIYPGYRFKAIDGLITNFHLPKSTLIMLVCAFAGREKVFKAYEEAVKEKYRFFSF
                     GDAMLIM"
     gene            complement(2470..3984)
                     /locus_tag="PK768_05860"
     CDS             complement(2470..3984)
                     /locus_tag="PK768_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HQA60530.1"
                     /translation="MKKYILFCICMVAFIIVPISTQSAELFPATVRVGLYYDSSARSM
                     YELTSDSGFNVMISNGQDNSTVSFSDKLLKVYTSDLDTFTSLKRNIRDKQAASEYASE
                     HAHNGADTYLLFDGTWSLWTKSNSNQFSSGGAYLVIKGDMKNPGLLIPFKGHQAVVFE
                     SRSPEGLIGVEGRRYRGILRMTPATGGNIQLVNELGLQEYLYGVVPLEVTPSWPEDFL
                     KAQAVAARTYTIANLSKWEKYGFDVGANSGDQLYGGYDAENLRTNKAVDETAGQVILY
                     KGEPIMAFYHADSGGRTEACNDVFSSDLPYLQPVDDIFYANSPHSEWETSLSLKDIND
                     RASAIVQEIGEIRQISIAERSNSGRVKKLLLIGKFGEKAIEKSQIRSILGLKSNFFDI
                     LGGDTVLAVAVSSENVKKDIRLKGNSVLTGQGKTRLSGGEIFMLAAETSKVLRTQASE
                     DVYIFRGRGFGHGVGMSQWGAKAMAENGYNYLDILLHYYKNTEIKSLTEVFYTR"
     gene            complement(4000..5007)
                     /gene="ruvB"
                     /locus_tag="PK768_05865"
     CDS             complement(4000..5007)
                     /gene="ruvB"
                     /locus_tag="PK768_05865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQA60531.1"
                     /translation="MIDRIVTPSVKNHEELDYEKSIRPLNFDEYIGQEKVKENLKVFI
                     KAALMRREALDHVLLFGPPGLGKTTLAYIIARELGVNIRITSGPAIERAGDLAAILTN
                     LGERDLLFIDEIHRLHPSVEEILYPAMEEFALDIMIGKGPSARSMRLSLSPFTLVGAT
                     TRAGLLTSPLRDRFGVNLRLDYYNISDLVKILKRSASLLKIQIEDEAARRLAVSSRGT
                     PRLANRLLKRIRDFAQVQGSGVITLDIAEEGLENLEIDSHGLDHNDRRLLSTIIDKFH
                     GGPVGIDSIAAALNEDVSTIEDVYEPYLIQKGYIFRTARGRVVSDKAYEHLGKEKATS
                     T"
     gene            complement(5020..>5248)
                     /locus_tag="PK768_05870"
     CDS             complement(5020..>5248)
                     /locus_tag="PK768_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQA60532.1"
                     /translation="KKTAQKIIIELKDRIKDLPVIADELRYNSVFEAKEVLISLGFNP
                     KEIQLALDAASIKSDDTVEDIVKKALKKLSK"
BASE COUNT         1409 a         1204 c          948 g         1687 t
ORIGIN      
        1 aacaccttca aacaaatctt ccggtgtgcc cacacccatc agatatctag gtttatcgta
       61 gggaaggaaa ggaactgtat attcgaggat ttcatacatt aactccttgg gttcacctac
      121 gcttaaccct cctattgcat atccgttaaa gccgagagag gttatgtctc tagcgctttt
      181 tcgccttaaa tctttgtaca taccgccctg aacaatacca aatagtgttt gcttgtctgg
      241 attacggtga gctttcagac atctttctgc ccatctcgtg gttctctcta ctgagtctaa
      301 tacataatcg taagtagcgg ggtacggtat gcactcatca aaagccatga taatatcgga
      361 ccctagtgca ttttgtattt caattgaggt ttcggggcct ataaaatgaa gggagccgtc
      421 gatgtgagat ctgaaagtta ctccttcttc ctttatttct cttaagtctc caaggctgaa
      481 aacctgaaag ccgccactat ccgtcaagat ggaaccgccc caattcataa acttgtgcaa
      541 tcccccggcc tcttttacca gttcgtggcc gggtcttaaa tatagatgat aagtattgct
      601 caaaattatt ttggctccta tttgctccaa ttcttccgga gtcatggtct taacagtagc
      661 ttgagtccca accggcataa aaacaggagt tttaaaacta ttatgaggag tctctataaa
      721 acctaatcta gctgaggtat tggtagattg ctttaaaact tcaaatttca tagtaatata
      781 tcatcctttt cttcggcttt tactacctta agttccctga ttaattcacc gtccatagat
      841 aaaatcgtta tttcagcgtc ggcaactcta aaggttttgc cgcgttcaag cttttctcta
      901 ctctcctgag agagttttga tactactcca cctacggtgt tgcctaaatc ctcaggaatg
      961 tttacatcta attttttatt gatatcctta acaagggtag caggatttac tatatttact
     1021 tcagtgataa aattccttgc ggggtcaaaa aatttgttga taaaaaacag cactccaatg
     1081 gcaaccacac ctaatagaat gtcagtcata gttttggact cgattatcat cttgcgggct
     1141 attgcataca gcaagacttc tattacactt gaaggtgtat gtctaatcag cataacagca
     1201 agttccaaac ctattaccag ggttaaaata tgccctagaa aggtttgcat caccggaaga
     1261 gtatcaatgg gagaagtgta gtataaaatt ctaaagtatc ctaatatatc ggtaaaactg
     1321 atgataacac cgaccgcaag caatgatgct agtattaact ctaagataat aacaattttt
     1381 gcaattctag aagacaattt cactcgaaga cacccacctt acattatcaa cattgcatct
     1441 ccaaaactaa aaaatctata cttttctttc acagcctctt catatgcttt gaagaccttc
     1501 tcccttcctg caaaagcaca gaccagcatt attagtgttg acttgggaag gtgaaagttg
     1561 gtaataaggc catctatggc tttaaatcta tatccgggat atataaagat gtcagaccac
     1621 ccgctacaag gctttatttt tccgtttaaa tctgcagagg tttctagggt ccgcgttgaa
     1681 gtagtgccga cagcgataac cctgcctcct gattcttttg tcgaattaat tacatccgca
     1741 gcatcctgac cgatttcata atattcggca tgcattttat gttcctcaat gttttctaca
     1801 gttacgggtc tgaaggtccc aagacctacg tgaagggtta tgtaagctgt tgagatgccc
     1861 ttttgcctta tattatcaag aagctcttct gtaaagtgaa gtcctgcagt cggtgccgct
     1921 gctgaaccgg gttttttggc atatactgtt tggtatcgac ttttttcctg caattttttc
     1981 tttatatacg gcggggtagg catctcacct aattcattta gaagtgtttg aaaaggtctt
     2041 ttgaaaaaaa actttacaat cctgcctcca taattagttg tgcctattac ctgagccgta
     2101 aggtctccat ctccaaacac aatttctgct cctgtgcgag ctctgcggcc gggtttaact
     2161 aatacctccc aggtatcagc ttcaacttct tttaacagca ccatttctat ttttccgagg
     2221 gtatgctttc gtcggccgta taacctagca ggtattacct tggtgtcgtt taataccaaa
     2281 caatctcccg gctgaaggta atgaataata tcttcaaaac tcttatgctc tattgtgcca
     2341 ttatttttat ttaataccaa taaccttgac tttgacctat tttcaattgg ttcctgagct
     2401 ataagctctt cgggaagata aaagtcaaac tcgtcaactt tcatggtctg ttacttcctt
     2461 aagtatattt tatcttgtat aaaagacttc ggttaaactt tttatttccg tgtttttata
     2521 gtagtgtagc aaaatgtcta ggtaattata cccgttttct gccatggctt tagcacccca
     2581 ttggctcatc ccgacgccat gaccaaagcc tcgacctcga aagatgtata catcttccga
     2641 tgcttgggtt cttaaaactt ttgatgtctc cgcagctaac ataaagattt ctccaccgga
     2701 taacctggtc ttcccttgtc ccgttaaaac actgttccct ttaagccgta tatccttctt
     2761 tacgttctcg ctggaaactg ccactgctaa aacagtatct cctcctaaaa tgtcaaaaaa
     2821 attgctcttt agccctagga tacttctgat ttggcttttt tctatagctt tttcaccaaa
     2881 ttttcctatc aggagcagtt tctttactct tccggaatta ctcctctctg cgatggatat
     2941 ttgacggatt tcacctatct cctgcacaat tgcacttgcc ctatcattta tatcctttaa
     3001 gctgaggctt gtttcccact ccgagtgggg agagtttgca taaaatatgt catctaccgg
     3061 ttgaagataa ggcaaatctg agctaaaaac atcattgcat gcctcagtgc ggccaccgct
     3121 gtctgcatga taaaaagcca ttattggctc acccttgtaa agtatgactt gaccggctgt
     3181 ttcatctacc gccttattag tccttaagtt ttccgcgtca tagccaccat acagttgatc
     3241 tccggagttt gcaccaacat cgaagccata cttttcccat ttagacaggt ttgctatcgt
     3301 ataagtcctg gccgccaccg cttgagcttt taaaaaatct tcaggccagc ttggtgtaac
     3361 ctccaggggt actaccccgt ataaatactc ttgaaggcca agctcattca ccaactgtat
     3421 atttccacca gtagccggcg tcatcctcaa aataccacga tatcttcgac cttcaacgcc
     3481 aattagacct tcagggcttc tgctttcaaa aaccacagct tgatggccct tgaagggaat
     3541 gagcaaaccg gggtttttca tatccccttt aataacaaga taagcaccgc cgctagagaa
     3601 ttgatttgaa ttgctttttg tccatagtga ccaagttcca tcaaagagca ggtaagtatc
     3661 tgcaccatta tgtgcatgtt ccgaggcata ttctgatgca gcttgcttat ccctgatatt
     3721 cctttttaaa gatgtaaagg tatccaaatc agaagtatat acttttagca gtttatcgga
     3781 gaagctcacc gtagagttat cctgcccatt gcttatcatc acattaaagc cgctatctga
     3841 ggtcaattcg tacatactgc gggcagaact atcataataa agacctactc taacagtagc
     3901 aggaaagagt tcagcagatt gtgtcgatat tggaactatg ataaatgcta ccatgcaaat
     3961 gcaaaataag atgtattttt tcatgattcg accccttcct catgtggaag ttgctttttc
     4021 tttaccaagg tgttcataag ccttatctga aacaactctt cctcgtgccg tcctgaagat
     4081 gtaacccttt tgaatcaggt atggttcgta tacgtcttca attgttgaga catcttcatt
     4141 cagcgcagcg gcaattgagt ctatccctac cggacctcca tgaaatttat ctattattgt
     4201 tgataataat ctcctgtcat tgtgatcgag accgtgactg tcaatttcta gattttccaa
     4261 gccttcctca gcaatatcaa gggtaattac cccgcttcct tggacttggg caaaatccct
     4321 gatgcgcttg agcagcctgt tggcaagtct aggtgtacct cgtgagctca cagctaaacg
     4381 ccgggcagct tcatcctcaa tttggatttt aaggaggctc gctgacctct ttaagatctt
     4441 taccaggtcg gatatattgt aataatctaa tcgcaaatta accccaaagc gatcccttaa
     4501 aggtgatgtt aatagtcctg ctcttgtggt tgcgcccacc aaggtaaagg ggctcaagct
     4561 taggcgcatg gatcgtgcac tggggccctt tccgatcata atgtctaggg caaattcttc
     4621 catggcagga tataagattt cctccacact tggatgaagg cgatgaattt catcgataaa
     4681 taaaaggtcc ctttccccta aattcgtaag tatggcggct aaatcccctg cccgttctat
     4741 agcaggtcct gaagttatcc ttatgtttac gcccagttct ctggctataa tatatgccag
     4801 ggttgtcttc cccaggccag gaggaccaaa taataaaacg tgatctagcg cttcacgtct
     4861 catcaaggca gccttaatga aaacctttag gttttccttc accttttcct gacctatgta
     4921 ctcatcaaag ttcaaaggtc taatactttt ttcgtagtct aattcctcat gatttttcac
     4981 agagggtgtc acaattcggt ctatcaattt attcaccctt tatttagaca attttttaag
     5041 tgccttcttt acaatatctt ccacagtatc atcgctttta attgaagcag catctaatgc
     5101 caattgtatt tccttcggat taaagcccaa ggaaatgagc acttctttag cttcaaacac
     5161 ggaattatat cttaattcat cggctattac gggcaaatct ttaatgcggt cttttaattc
     5221 tatgatgatt ttttgagctg ttttttta
//
LOCUS       DAONUO010000132         5239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11539_length_5239_cov_4.319637, whole genome shotgun sequence.
ACCESSION   DAONUO010000132 DAONUO010000000
VERSION     DAONUO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11539_length_5239_cov_4.319637"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PK768_05875"
     CDS             <1..739
                     /locus_tag="PK768_05875"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQA60533.1"
                     /translation="AASAITGVITDPRRLNKKPLDFKIPDKFIIDKSIFIIPEKDPKK
                     KVRKGPNIKPIPEFPEMEDITRGKVILKLGNDVTTDLICPAGALYLPIRSNIPEMSKH
                     SFKLVDETFYERAKAFGKSIIVAGHNYGQGSSREQAAIIPRYLGINVIMAKSFARLHL
                     ANLVNWGILPLIFTNDEDYNSVDRDDLLSVETINLKVGEPVKVVNLSKGKTMYLSTPL
                     FQEDLDAIKKGGFVNVLKDKFKNKGEN"
     gene            752..1963
                     /locus_tag="PK768_05880"
     CDS             752..1963
                     /locus_tag="PK768_05880"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQA60534.1"
                     /translation="MSRIKMKNPIVEIDGDEFARIIWNWVKDILIMPHVDLKTEYYDL
                     HIKNRDNTNDQVTIDAADAIKKHGVGVKCATITANLDRQKEYNLKDIYKSPNATIRKI
                     LGGTSFRKPIILETIKSTVPNWKKPIIVARHTFGDLYASLSMEIEAGTKVEIVLRDEQ
                     NRESRTPFAHFEEDGILLCQYNLENSIYNFAESCFKYAMEEKLDLLFSAKDTVTKAYD
                     TKFKSIFEELYETKYREMFAELGIKYQYSLIDNAIAKILRSEGGIVWACKNYEGDLMS
                     DMVAAGFSSLGLMTSVLISPEGMYEFEAAHGTIQDLYYKHLKGEKTSANPTATIFAWT
                     GALRKRGALDGNDDLVMFAKRLEDTTLLTLNSGFITEDIFNISTASNKRICTSEEIID
                     IIASNLEKSYK"
     gene            complement(2030..2572)
                     /gene="citX"
                     /locus_tag="PK768_05885"
     CDS             complement(2030..2572)
                     /gene="citX"
                     /locus_tag="PK768_05885"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HQA60535.1"
                     /translation="MDVMPVDIKILQSILDSREARAEKQKEILKKYPHTLISFTLNTP
                     GAKKSSDLYSKVHKVGMKAILKSLRDNNKKVYFAQSYDYPTGPEGFISVDMDPIEAKK
                     MVVELENNHELGRIFDVDVFDKNHNQISRSMLNLAPRKCLICEKAARYCIRTRAHTYE
                     ELIKKVEEMSLNYFSNKEKP"
     gene            complement(2578..3465)
                     /gene="citG"
                     /locus_tag="PK768_05890"
     CDS             complement(2578..3465)
                     /gene="citG"
                     /locus_tag="PK768_05890"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR03125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HQA60536.1"
                     /translation="MKGRNFCFYITKLAEKALLYEVSATPKPGLVDRNNSGAHHDMDY
                     YTFLNSSVALMDYFYNCTLAGLTFNEPYYDRLLDKIRPIGIEAELSMYEATKGVNTHK
                     GIIFSLGIIAAAAGLLYSINGSKFIPAPEISKLVRQICKGITEELKNTGHKKSLTYGE
                     RIFKKYGVKGIRGEVESGFQTVLQHSLPLIKELMKEKRTINDVTVTILLALIAHTEDS
                     NILGRHNMEMLEYAQEKARAALNLGGYLTPEGKEFVWEMDRDFIEKNISPGGAADLTA
                     VTLFLYFLENGDNLIKTKG"
     gene            complement(3496..4434)
                     /locus_tag="PK768_05895"
     CDS             complement(3496..4434)
                     /locus_tag="PK768_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQA60537.1"
                     /translation="MNDRELLYVKTIADTKSISEASKKLFVTQPSLSQALQKIEDRLG
                     TPLFIRYQNGVKLTLAGEKYYRAATEILKIYNELCNEITEINNLKKGKITIGVTNYLA
                     VYLLPIILPKFRKKWPNIEIYIHEDNSYNIEDLLMKGSVDFAIMHYESDDDNKSLKYD
                     ALCEDFFVLVTKRNHPLSKYAEKVEGLDYPVIDLHLFKDENFVLLERDKRIRQICDSI
                     FEELNFKPKIALLLRNYETARRLASVGFGVTCIPFGYINIFEGEYDADYYFIKGNNKT
                     SWKTCIVTNPNNYISKAARVFINLVKEYYRTHPVTI"
     gene            4771..>5239
                     /locus_tag="PK768_05900"
     CDS             4771..>5239
                     /locus_tag="PK768_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQA60538.1"
                     /translation="MDAKRLKGTKTEKNLLEAFAGEAMARCKYDYYAAIAKKEGYEQI
                     MGVFQETAYNEKEHAKMWAKFLGLIGDTEKNLESAANDENYEWTKMYKAFAETAREEG
                     FDDIAKFFEKVAEVEAEHEERFRKLLERVKEGSVFKRSEPIKWRCRNCGYIHEG"
BASE COUNT         1621 a         1037 c         1003 g         1578 t
ORIGIN      
        1 tgcagcttct gctataaccg gggtgataac ggaccccaga agattaaaca aaaagccatt
       61 agattttaaa attcctgata aattcataat agacaagagt atattcataa taccggaaaa
      121 ggatcccaag aaaaaagtca ggaaggggcc aaatataaag cctattccgg aatttcctga
      181 aatggaagat ataacaaggg gaaaggttat attaaaactc ggaaacgatg tgacaactga
      241 cctcatttgc cctgccggag ctctatacct tccgataagg tctaatatcc cagaaatgtc
      301 aaaacactca tttaagctgg tagatgaaac attttacgaa agggcgaagg ctttcggcaa
      361 atccataatt gtagcaggcc ataactacgg acaaggttca agcagggaac aggctgccat
      421 aatacccagg tacctaggga taaatgttat tatggctaag agctttgctc ggctgcatct
      481 tgccaattta gtaaactggg gcatcctgcc cttgatattt acaaatgatg aagattacaa
      541 ttctgttgac agagatgacc tgctatcggt tgagacgatt aatctaaaag tgggtgagcc
      601 ggttaaagtt gtaaacctat ccaagggcaa aaccatgtac ttaagtactc cccttttcca
      661 ggaagatctg gatgccatta aaaaaggcgg ctttgtcaat gtgttaaaag acaagttcaa
      721 aaacaagggg gaaaactagg gaggcaatga tatgtccagg ataaaaatga aaaaccctat
      781 tgtggaaata gacggagatg aatttgctag aatcatatgg aactgggtta aagatatatt
      841 aattatgccc cacgttgatt taaaaactga atattacgat ctacacataa aaaacagaga
      901 caatactaac gatcaagtta caatagatgc tgccgatgcc ataaaaaaac acggtgttgg
      961 cgtaaaatgt gccaccataa ctgcaaatct ggacagacaa aaggaataca atctaaaaga
     1021 tatatacaaa agtcccaatg ccactatcag gaagatttta ggcggaacat cctttagaaa
     1081 gcccataata ctggagacca ttaaatcaac agttcctaat tggaaaaagc cgataattgt
     1141 agcgagacat accttcggtg acctgtatgc ttccttaagc atggaaatcg aagccggaac
     1201 aaaggtggaa attgtgttaa gggatgaaca aaaccgcgaa agcaggactc cctttgccca
     1261 ctttgaagaa gacggtattc tgctttgcca atataatctg gaaaattcca tttataactt
     1321 tgcagaatcc tgttttaagt atgccatgga agaaaagctt gacctgctat tttcggcaaa
     1381 agacacggtg acaaaagctt acgatacaaa atttaaaagt atctttgaag agttatacga
     1441 aacgaaatac agggaaatgt ttgctgagct ggggataaag taccaatatt ctttgatcga
     1501 taatgcaatt gcaaagatac taaggtctga aggcggcatc gtctgggcct gcaaaaacta
     1561 tgaaggtgac ttaatgagcg atatggttgc tgcaggtttt agcagtttag gccttatgac
     1621 atccgtactg atatcccccg aaggcatgta cgagtttgaa gctgctcacg gcacaattca
     1681 ggatttgtat tacaaacact taaagggaga aaagacctcc gcaaatccta cagccaccat
     1741 ttttgcctgg acgggtgccc ttagaaaacg cggagcactg gacggaaatg acgatttagt
     1801 aatgtttgca aaacgtttag aagatacaac actattaact ttaaacagcg gctttataac
     1861 tgaagatatt ttcaatatat ctacggcgtc caataagcgc atttgcacca gcgaagagat
     1921 aatcgatatt attgcttcga atttggaaaa atcctataag taaaaagttc cccgaacata
     1981 ataagtcgac cggtaaatcc cctcaatggc ttttaccggt cgattaaatt taaggctttt
     2041 ccttatttga aaaatagttg agactcattt cctcaacttt tttaataagt tcttcataag
     2101 tatgcgctct agttctaatg cagtaccttg ccgctttttc acaaatcaaa cattttcttg
     2161 gagccagatt tagcatggac ctgctgattt gattgtggtt tttatcaaaa acatccacgt
     2221 caaatatgcg tcccaattca tggttgtttt ccagttccac caccattttt ttcgcctcaa
     2281 ttggatccat atctactgat ataaaccctt ccgggcccgt tggataatca tatgactgtg
     2341 caaaatatac cttcttattg ttatctcgaa gactttttaa aatggccttc atgcctactt
     2401 tgtgtacttt agagtataag tctgaactct ttttcgctcc aggtgtattt aaggtaaaag
     2461 aaatcagcgt gtgaggatat tttttaagaa tctccttttg tttttcagct cttgcttcgc
     2521 gggagtccaa gatgctttgt aagattttta tatccacagg cataacgtcc atgcggttca
     2581 acctttcgtt tttatgagat tgtctccgtt ttctagaaaa tataaaaaca gcgtgacagc
     2641 cgttaaatca gctgccccac ccgggcttat gttcttctca ataaagtccc tatccatttc
     2701 ccaaacaaac tctttacctt caggggtaag gtatccgccc aagtttaagg cagcccttgc
     2761 cttctcttgg gcatattcga gcatttccat gttgtgccgt cctagaatgt tgctgtcctc
     2821 ggtgtgtgcg ataagtgcca ggagtatagt tactgtaaca tcattaatag ttcttttttc
     2881 tttcattagc tcttttatca gtggcagtga atgctgcagc accgtttgaa atccgctttc
     2941 cacttccccc ctgattccct taacaccata ttttttaaaa attctctctc cgtacgttaa
     3001 gctttttttg tgaccggtat tttttaactc ttccgttata cctttgcaaa tttgcctgac
     3061 aagctttgat atttccggag caggaataaa tttagagcca ttaatactgt aaagcagacc
     3121 cgcagctgct gctataatcc ccagggagaa tatgattccc ttatgggtgt tcactccctt
     3181 tgtagcttcg tacatactga gttccgcctc aatcccaatc ggccttattt tatctaacaa
     3241 cctatcgtaa taaggctcat tgaaagtcag gcctgctaaa gtgcaattat aaaaataatc
     3301 catcaaggct acgctactgt ttaaaaaagt ataataatcc atgtcatggt gggcccctga
     3361 attatttctg tctaccaggc ccggtttagg agttgctgaa acctcatata aaagtgcctt
     3421 ttcagcaagt ttcgttatgt aaaagcaaaa attacgtccc ttcaatctcc agctctcctt
     3481 gctgccaact ttattttaaa tcgtaacggg atgagtcctg tagtattcct tcaccaggtt
     3541 aataaagacc cttgccgctt tggatatata attattagga ttggtcacta tgcatgtttt
     3601 ccaggaagtt ttattattcc cttttataaa ataataatct gcatcatatt caccttcaaa
     3661 tatatttatg tacccgaaag gtatgcatgt tacgccgaat cccacacttg caagcctccg
     3721 tgctgtttcg taattcctga gcagcaaagc tatcttaggt ttaaaattca attcttcaaa
     3781 tatgctatca caaatttgcc tgatcctctt gtccctttcg agtaacacaa agttctcatc
     3841 tttgaaaagg tgtaaatcaa tgaccgggta atccaaacct tccacttttt cagcatactt
     3901 ggacaagggg tgattgcgtt tagttacgag tacgaaaaaa tcttcacaca aagcatcata
     3961 tttcagcgat ttgttatcat catccgattc ataatgcatg attgcgaaat cgaccgaacc
     4021 tttcattaaa aggtcctcga tgttgtaaga attgtcctca tgaatataaa tctcgatatt
     4081 aggccatttt ttgcgaaact tgggcaggat tattggaagg aggtatacag ccaggtagtt
     4141 tgtaacgccg attgtgattt tccctttttt taagttgttt atttcggtta tctcgttgca
     4201 caattcgtta taaatcttca atatttccgt tgctgcccta tagtattttt caccagctag
     4261 ggtcaacttt acaccatttt gatatctaat aaatagagga gtgcccaatc tatcttcaat
     4321 cttttgtaaa gcctgactca atgagggctg tgtcacaaaa agtttttttg acgcttcaga
     4381 tatgctcttg gtgtcagcta ttgttttgac atataacaac tccctgtcat tcatgaaagt
     4441 tcctcctttg tagccgggag caattatccc cccttgttat tagactttct tatcttatcc
     4501 tatacagttt tcaaggaact attaaagctc tttgattttt tcggcacacc ttgcaaaatg
     4561 gtgaaaacaa tttcaatatg gcaggttaat tatataataa aatactatca ttttcaaata
     4621 ttatggcttg cccgtcattt tatgctattg ttctggcaat attatgcaca attcagagat
     4681 tataaataat agctattaag aaggactttt tacttttatg tcgaagttta agtaagaata
     4741 attattattt gcgatattgg aggtaattta atggatgcaa aaaggttaaa aggcactaag
     4801 acggagaaaa acttgctgga agcatttgca ggagaagcta tggcccgctg caaatacgat
     4861 tactatgctg ctattgcaaa aaaggaaggc tatgaacaaa taatgggggt tttccaggag
     4921 accgcctata atgaaaaaga acacgccaaa atgtgggcaa agtttttggg attaataggc
     4981 gataccgaaa agaaccttga atcagcagca aatgacgaaa actacgagtg gactaaaatg
     5041 tacaaagcat ttgccgaaac tgctcgggaa gaaggctttg acgatatcgc caagtttttc
     5101 gaaaaagttg ctgaagttga agccgagcat gaagaaagat ttcgcaagct tctcgagcgc
     5161 gtaaaagaag gttccgtatt taaacgttcc gaacctataa agtggcgttg ccgcaactgc
     5221 ggttatattc atgaaggca
//
LOCUS       DAONUO010000133         5195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11655_length_5195_cov_3.924708, whole genome shotgun sequence.
ACCESSION   DAONUO010000133 DAONUO010000000
VERSION     DAONUO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11655_length_5195_cov_3.924708"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PK768_05905"
     CDS             <1..1125
                     /locus_tag="PK768_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA60539.1"
                     /translation="MLEVDPDKLVKDCPVGVQQRIEILKILHQNADILIFDEPSSVLT
                     PIEVDELLKTMKRLSNMGKSIILITHKLGEVMEVADRIVVMRDGEVVAEKRREETSIE
                     ELSYFMIGRQTTSPVIKDLEPCKEVLSVKNLSKVDESGKKLLGNINISVRGGEIVGIA
                     GVSGNGQSELIKCITGLDTDYTGKIEVCGINVTNKGVGFIRKAGLAHIPEDRYYWGSA
                     KEATIAENVLMGDENTERFSKKGLLNFREIRKHVYRIVENYGVKVSFLAQKMKELSGG
                     NAQKLIVAREIEKKTPFLVACEPTRGIDVGAMEFIHEKLIEKRNNGGGILLISSELSE
                     ILKLSDRIYVIYEGRIEHEFTNKNLDSRKLGFLMLGGKTA"
     gene            1125..2225
                     /locus_tag="PK768_05910"
     CDS             1125..2225
                     /locus_tag="PK768_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60540.1"
                     /translation="MEVKKINFIYYVQKNLIESVKLPFISITLGLIVGGIIIALSGGD
                     PFAAIKGLIVGGFGSMHAITVTLTRATPIIFAGLSAALAWGSGYSSMGASGQMILGAF
                     TAAITAVSISGPSIVIIIVSLSAGMLAGIIYSLIAAYISAKFEVYLLIVTLMMNYIAD
                     NIASYLTTYDFRDPLAVDRLAVQTQKIIDGTLPRIFPRYTLHYGFIIAIACTLLVLFM
                     MKRTSFGYEAKMSGLNPRFADYGGIDSLKTMYLVLMISGALAGLGGACESLGTRFRYV
                     DGMITSGGYAWSGIIASLMASNHPVGVFVSSVFLAGLTTGGGSIELRHGIPSEISDII
                     EGVLTMFVTAKLVVDFRFGRLRAQKEDGADVV"
     gene            2215..3162
                     /locus_tag="PK768_05915"
     CDS             2215..3162
                     /locus_tag="PK768_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60541.1"
                     /translation="MLFSIDYIATIINSTIRMMSPLLYASLAAAICAKADIFNLSMEG
                     AMLVSAFFSIVVNYFTKSVVLSILAGVMASVLVSACVGFFVLKLKASPVVVGLAINNT
                     VAGATTYLLYIIFNTRGVFTHPSLVGIPKIRLPFIEKTAVLSTIFSGLTLLDYMSFVI
                     AILVYLFFYKTVIGYRIRAIGINSEAARSLGTPVEKYQFLTISFSGILCGFAGCLLSM
                     GSVRLFIENITAGRGYIAMAANHLGKSHPIGVLFASLFFGCCQALGNFLQNTSLKTQI
                     TSSIPYVATILALIVFTVQKRYAKQKKMLEVFNKDAASS"
     gene            3146..3868
                     /locus_tag="PK768_05920"
     CDS             3146..3868
                     /locus_tag="PK768_05920"
                     /inference="COORDINATES: protein motif:HMM:NF006702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HQA60542.1"
                     /translation="MRLLVDPHTHTIASAHAYSTILENAQAAAKRGIKVLGITDHAPA
                     LEDAPHVLYFKNLHVLDRELYGVKMLYGAELNICDYEGRVDLDEQILENLDFCIASFH
                     TVLLKAGSKNQNTRAMLGAMDNPYVKIIGHPEDGNIPVDYVELVKHARETGTMLEVNN
                     SSLKTAYYRLNVRENLMEMLQLCEKYGVPIAVGSDAHFANAVGNFDCAIKILEEISFP
                     KELVANTSVEKFLKLIGREVKV"
     gene            3865..4680
                     /locus_tag="PK768_05925"
     CDS             3865..4680
                     /locus_tag="PK768_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA60543.1"
                     /translation="MKRCFLHLSDIHYLIDYTKCNSFYAEIFKELTPPVEQIRSLVKE
                     VNVTDIDFVLISGDLTENGNDEDYKHLKMHLREIFGNIPIIVTLGNHDNIKEFNKGWL
                     GSVGINESSYHVLVNEAGINIISLDSSSEKHPDGMITEADCDWLEEAIQDSKNEPTIL
                     LTHHHLLDSQFSMPRAQYPEKFIQLVRDSNIIAVFNGHTHHFYSGEFAGKPYFTADSV
                     SFSAEYVSDNHVYFVQRPGLTFFCLENGKIIPNRIGGIKISKYFGIFKPKEEI"
     gene            complement(4728..5036)
                     /locus_tag="PK768_05930"
     CDS             complement(4728..5036)
                     /locus_tag="PK768_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HQA60544.1"
                     /translation="MNEKSSSKSKKAVALRYDSAKDTAPKIVASGKGLLAEKILELAR
                     KENIPVYKDPHLVEALIQLDINVEIPSELYRAVAEILAFIYTVDAANNPGGKFYDENS
                     "
BASE COUNT         1571 a          918 c         1118 g         1588 t
ORIGIN      
        1 atgctagaag tcgatccaga taaattagtt aaagactgtc ctgttggcgt tcaacagcga
       61 attgagattc tcaaaatact tcatcagaat gcagacattc tcatctttga tgaaccttct
      121 tctgtattaa ccccaattga ggtagacgaa ttattaaaga caatgaaacg cctgtcaaat
      181 atgggaaaga gtataatact tatcacacat aagctaggag aagttatgga agtggctgat
      241 cgcattgtgg ttatgagaga tggagaagta gttgcagaaa aacgtagaga agagacctca
      301 atagaggaat tatcttactt tatgataggc cgtcaaacta catctcccgt tattaaggat
      361 ttagagcctt gcaaagaggt attatctgta aaaaatttgt ctaaggtaga tgaatccggc
      421 aagaaattgc taggcaacat taatatatca gttaggggag gggagatagt agggattgcg
      481 ggagtatcag gcaacggcca aagtgagcta ataaagtgta ttaccggtct tgacactgat
      541 tatacaggta aaatagaagt ctgtggaatc aatgtgacaa ataaaggtgt aggatttatc
      601 agaaaagcag gccttgccca tattcctgag gatagatatt attggggaag cgcaaaagaa
      661 gcaactatag ctgaaaatgt tttaatgggt gatgaaaaca cagagcggtt ctcaaaaaaa
      721 ggcctcctta atttcagaga gataagaaaa catgtctata gaattgttga aaactatggg
      781 gtaaaagtaa gttttctggc acaaaagatg aaagagcttt ctggggggaa tgcacaaaag
      841 cttatagttg ccagggaaat tgaaaagaaa acaccttttc tggttgcttg tgagcctact
      901 cgcggtatag acgttggtgc tatggaattt attcacgaga aattgatcga aaagaggaat
      961 aatggaggtg gaattttact gatttcttca gaacttagcg aaatattaaa actaagcgac
     1021 aggatatatg tcatatacga aggtagaatt gaacacgaat ttacaaataa aaacctggac
     1081 agtcgcaagc ttggcttttt aatgttaggg ggtaagactg cataatggag gttaaaaaaa
     1141 tcaattttat ctattatgtg caaaaaaacc tgattgaatc tgtaaaactt cctttcatat
     1201 ctatcaccct cggcctaatt gtgggaggga taataatcgc cctttccggc ggagatccct
     1261 ttgctgccat aaaagggctg atagtcggcg gattcggctc aatgcacgct attactgtaa
     1321 cattaacccg tgccacacct attatttttg caggcctttc cgctgctttg gcatggggtt
     1381 caggatattc cagcatgggg gcatcaggtc aaatgatact tggagcattc acagcagcta
     1441 ttacagcagt atctatttct ggtccttcaa ttgtaataat cattgtttct ctctccgcag
     1501 gaatgctagc tggcatcatt tattctctta ttgctgctta tatcagcgcc aagtttgaag
     1561 tttatctgct catagtcacc ctgatgatga attatatcgc agataatata gcatcttact
     1621 tgactacata cgattttcga gaccccttgg ctgtagacag actggcagta caaacccaaa
     1681 aaattataga tggtactttg cctcggatat ttcctagata tacgttgcac tatggattta
     1741 taattgcaat agcatgtacc ctcctggtgc tatttatgat gaagcgcacc agctttggat
     1801 acgaagccaa gatgagtggt ttaaatccca gatttgctga ttatgggggc attgacagtt
     1861 taaaaaccat gtatttggtt ttaatgataa gcggcgccct agcaggcctt ggaggggctt
     1921 gcgaatccct tggtaccagg tttagatatg tggatgggat gatcacatcc ggtggatacg
     1981 cgtggagcgg cataatcgca tcccttatgg cttcaaatca tccagtggga gtttttgtca
     2041 gttcagtttt ccttgccggc ctgacaactg gaggcggttc cattgaattg agacatggaa
     2101 tacccagcga aatatctgac ataattgaag gtgttttgac catgtttgtt actgcaaaac
     2161 ttgtcgttga ttttagattt ggtcgtctca gggctcaaaa ggaggatgga gctgatgttg
     2221 tttagcatag actatatcgc tacaatcatt aattccacta ttcgaatgat gtcacccttg
     2281 ctttatgcct ctctggcagc ggctatttgc gctaaagcgg atattttcaa cctatccatg
     2341 gaaggagcaa tgcttgtaag tgcttttttt agtatagtgg taaattattt taccaaaagt
     2401 gttgtgttat caatattagc tggtgtgatg gctagtgttt tggtatcggc ctgtgtaggt
     2461 ttttttgtgc tgaaacttaa agcatctcct gttgttgttg gtcttgcgat taataacacc
     2521 gtagccggag caacaactta cttgttatat attattttca atacaagagg tgtttttact
     2581 catcccagct tggttggaat acctaaaata cgcctccctt ttattgaaaa aactgcagtt
     2641 ttgtcaacta ttttttcagg actgacatta cttgattata tgtcttttgt aatcgcaata
     2701 cttgtatatt tattttttta caaaacggtt atagggtacc gaatcagagc tattggtatc
     2761 aacagtgaag ctgcgcgcag cctggggaca ccagtcgaaa agtatcaatt tttaactatt
     2821 agtttttcgg gaatactgtg tggttttgcg ggatgtttgt tgtcgatggg ttcggtgaga
     2881 ttatttattg aaaatattac tgctggacgc ggttacatag caatggctgc aaatcatctg
     2941 gggaaatccc atcccattgg agtccttttc gccagtttgt ttttcggatg ctgccaagct
     3001 cttgggaatt ttttacaaaa cacctccctt aaaactcaga taacatcttc tattccttat
     3061 gtggcaacca tcttagctct tatagttttc actgttcaaa agagatatgc aaaacaaaaa
     3121 aagatgctgg aggtgtttaa taaagatgcg gcttctagtt gatccccata cacacacgat
     3181 agcaagcgca catgcgtatt caacgatact agaaaatgcg caagctgctg caaagcgagg
     3241 tattaaggtc cttgggataa cggatcatgc acctgcatta gaagatgcac cccatgtttt
     3301 atattttaaa aatttacatg tcttggatag ggagctatac ggtgtaaaaa tgctctacgg
     3361 agctgaactt aatatttgcg actatgaagg tagagtagac ttagatgaac agatacttga
     3421 aaacttggac ttttgcatag cgagcttcca tacagttctt ttaaaagcag gttcgaaaaa
     3481 ccaaaatacc agagccatgt taggagcgat ggacaacccc tatgttaaga ttataggtca
     3541 tcctgaagat ggcaatattc ccgtagatta tgtcgagtta gtaaagcatg caagagagac
     3601 gggcacgatg ttggaagtaa acaactcatc cctcaagaca gcatactacc gcctaaatgt
     3661 gcgagaaaat ctcatggaaa tgctacagct ttgtgaaaaa tacggtgttc cgattgctgt
     3721 cggctctgat gcacattttg caaatgctgt tggtaacttt gattgtgcca ttaaaattct
     3781 tgaggaaata agttttccca aagagcttgt agcaaatacc tccgtggaga aatttttaaa
     3841 actcattggg cgagaggtga aggtatgaag cgctgttttt tacacttgtc tgatatccat
     3901 tacctaattg attatactaa atgtaatagc ttttatgcag agatattcaa agagttaaca
     3961 ccgcctgtag aacagatcag aagtcttgtt aaggaagtca atgtcactga tatcgacttt
     4021 gtgctcataa gcggggatct tacagaaaat ggtaatgacg aggattataa acatttgaaa
     4081 atgcatctta gggagatatt cggcaatata cctataatag taacattagg aaatcatgat
     4141 aatatcaaag agtttaataa aggctggctt ggaagtgtag gtataaatga gagttcatac
     4201 catgtacttg tcaatgaggc aggaataaat ataatttccc ttgacagctc ctcagaaaaa
     4261 catccagatg gcatgataac agaagctgac tgtgattggc tagaagaggc aatacaagat
     4321 tcaaaaaatg aacctacaat tctgcttaca catcatcatc tgttggacag ccaattttca
     4381 atgcccaggg cacagtaccc tgaaaagttc attcagctag taagagatag taatattata
     4441 gcggttttca atggccatac acatcatttt tacagtggag aatttgctgg taaaccgtat
     4501 tttacagcag atagtgtttc tttttctgca gaatatgtca gtgataatca tgtttacttt
     4561 gtgcagcgac ccggattgac ttttttctgt ctagaaaatg ggaaaattat accaaataga
     4621 ataggaggaa taaaaattag taaatatttt ggtattttta aacctaaaga ggagatttaa
     4681 tgagaaatgc acagtttaaa aaaatcagga ttaaaagtat ttacttacta actattctca
     4741 tcgtagaatt ttccaccagg attgtttgcg gcatcaacag tgtaaataaa ggccaggatc
     4801 tcggctacgg ccctgtatag ttcagagggt atttccacgt tgatgtcaag ctgaatcaag
     4861 gcttctacca gatggggatc cttatatacg ggtatgtttt ccttccgggc aagctcaagg
     4921 attttttctg ccagtaggcc ttttccggaa gcaacgattt ttggagccgt gtctttggcg
     4981 ctgtcgtatc tcaaggcaac agccttttta ctttttgagc ttgatttttc attcatgtta
     5041 tcaccgacct tatgattaaa cctttatatt tatacccgta gttatcggct gcttttttat
     5101 atccccaaaa aagaagtctc ttatggtgat cctccccacc tttagcttca tggctttgat
     5161 gacattcggg atatccctat tctcatttaa aaact
//
LOCUS       DAONUO010000134         5181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11691_length_5181_cov_3.396996, whole genome shotgun sequence.
ACCESSION   DAONUO010000134 DAONUO010000000
VERSION     DAONUO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11691_length_5181_cov_3.396996"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PK768_05935"
     CDS             <1..397
                     /locus_tag="PK768_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein SpoIID"
                     /protein_id="HQA60545.1"
                     /translation="GPEGRRTWSASFSKDEISSVIRAEFGLETGPIDSVSVEERGPSG
                     RATKLKIGDAVVSAPELRIALGSMEMRSTLLTSFKVEGDKVLMAGKGFGHGVGLSQWG
                     ANVMAKQKTKPEDIIRHYFKDVDIVKLWK"
     gene            458..733
                     /gene="yabP"
                     /locus_tag="PK768_05940"
     CDS             458..733
                     /gene="yabP"
                     /locus_tag="PK768_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HQA60546.1"
                     /translation="MEEKGGHKITLTNRELLDVSGVLNVEKFTDEDVLLETDQGMLNI
                     KGENMHMKQLNLDAGMIAIEGAFKALSYQEGLALGQKRKSLLRRLLK"
     gene            755..1192
                     /locus_tag="PK768_05945"
     CDS             755..1192
                     /locus_tag="PK768_05945"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HQA60547.1"
                     /translation="MGLWQLQPQIMLLTASLVIGLLTGLLFDIYRRIRNLVSPGPYLT
                     ALGDLCFWILISAITFASLFNISSGQVRGYVFASIAAGLAFYMHFISRYVIYFFVVLH
                     NYFGIGFLKISKSIGRVKRLKSVRLMRRLWQDTRRILSKIRRK"
     gene            1255..1542
                     /locus_tag="PK768_05950"
     CDS             1255..1542
                     /locus_tag="PK768_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQA60548.1"
                     /translation="MAEKRKLRIRHLLLLFFLIYIMFTLVSQQFKIAELARKDAELEA
                     EKTALLQEREELKKEISLLHTDEYIEGIARDELGLVKPGEYIIKSSRSSKR"
     gene            1675..2055
                     /locus_tag="PK768_05955"
     CDS             1675..2055
                     /locus_tag="PK768_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQA60549.1"
                     /translation="MPVEVGQIVEGRITGITRFGAFMEIAQGVNGLIHISEVADSFVK
                     DVNDYLKENDIVKAKVISISKDGKISLSLRKANENAKTKQVDQSFEDKLAKFLKDSEE
                     RMLDIKRYHESKRGSGSYGRRKVL"
     gene            2132..3046
                     /locus_tag="PK768_05960"
     CDS             2132..3046
                     /locus_tag="PK768_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HQA60550.1"
                     /translation="MRVAVIDLGSNSIRLLVKDLWASSMATVHKELATTRLGRGVVRS
                     SELDRQSMADTLSVIAEFISRANSMGTERILAFGTSALREAKNSSGFIKKVNELGLDV
                     DILSGEEEALLNFKGARAGLGLSETTLAIDIGGGSTELILGKERIQKSASLPVGAVRF
                     TELYFNSEPPNPKDVKAAGDEIFRRCLDFARFYNRTKSGHAIVAVGVGGTLTTLAAMD
                     QELVIYDPEKIHGYALKKLSIDDIYTKLLTLTLDEKRKLPGLMPQRADIITAGTFIAK
                     TIMEHLEISEIIISETDIMEGYLLYKLA"
     gene            3369..>5181
                     /locus_tag="PK768_05965"
     CDS             3369..>5181
                     /locus_tag="PK768_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQA60551.1"
                     /translation="MVQPITIGNRTRWNFSKIDEVMGMPNLIEIQKDSYQWFLNEGIR
                     EVFNDISPIEDFTGNLVLEFIEHKLESTPKYSEDEARERDVTYAVPLKVKVRLINKET
                     GEVKEQEVFMGDFPLMTENGTFIINGAERVIVSQLVRSPGVYFDSHRDKNGRELFSGT
                     VIPNRGAWLEMEMDSNDVLYVRIDRTRKLPITVLLRALGYGTNAQILELMGEDEKILR
                     TLEKDNTENENEALIEIYKRLRPGEPPTVENARSLLESLFFDNKRYDLAHVGRYKFNK
                     KLRLRDRIVSKVSAEDVENPETGELVLKKGEKISRKTAELLESLKIKELKIIMPEDSQ
                     EARLRADYSPKVAKVISNGYSEEPGPEGNRTEKHITVEDIVAAVNYFVNLPYKIGKID
                     DIDHLGNRRLRSVGELLQNQFRIGLARMERVIKERMTIQDVDTITPQGLINIRPVVAA
                     VKEFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLSRDRAGFEVRDVHHSHYGRMC
                     PIETPEGPNIGLIGSLSTYARVNEYGFIETPYRKVDKERGVVTDEIVYLTADEEDEYT
                     IAQANAPLDEEGRFIQKRVVARYADEIIVVPIEDVDYM"
BASE COUNT         1575 a         1038 c         1313 g         1255 t
ORIGIN      
        1 ggggccggaa ggcaggcgga cctggtcggc gtctttttca aaagatgaga taagctccgt
       61 aataagggcc gagtttggcc tggaaacggg tcccatagac agcgtcagcg tagaggagcg
      121 gggcccttcg ggccgggcga cgaagcttaa aataggagat gctgttgttt cggcgccgga
      181 acttcgcata gccctaggaa gcatggaaat gaggtcaacc ctgctaacga gtttcaaagt
      241 ggagggcgat aaggtactaa tggcgggaaa gggtttcggc cacggtgtag gtctttccca
      301 gtggggcgcc aatgtcatgg ccaaacaaaa aactaagccc gaagatataa tacgccatta
      361 tttcaaagat gtagacatag taaagctgtg gaaataacat attttatact cctcccgaat
      421 aaaattaaag gaaggattaa taaggggagg gtttgtcgtg gaagagaagg gcggtcacaa
      481 aataacactt accaatcggg agttgttaga tgtaagcggc gttctcaacg tagaaaagtt
      541 caccgatgag gatgtgctcc tggaaactga ccagggaatg ctcaacatca agggcgaaaa
      601 catgcacatg aaacaactga atttggatgc cggcatgatt gccattgagg gtgcttttaa
      661 ggcgcttagc taccaggaag gcttggcctt ggggcaaaag aggaaaagcc tgctcaggcg
      721 cctgctcaag taggccggaa ggaggatggt atgagtgggc ctttggcagc tacagcctca
      781 aatcatgctc ttgacggcat ccttggtaat aggactgctt acgggactcc tatttgacat
      841 atatcggcga atcaggaact tggtctcccc ggggccttac ctaacagctc tcggggattt
      901 gtgtttttgg attctcattt ccgccatcac ctttgcatct ctctttaaca tcagttccgg
      961 gcaagtcagg gggtatgttt ttgccagcat tgcagctggc ttagcctttt atatgcattt
     1021 tatcagccgc tatgtaatat acttctttgt cgtattacac aattattttg gaattggctt
     1081 tctcaagata tcaaaatcta tcggcagggt aaaaaggtta aagtctgtac gtctaatgcg
     1141 gaggctctgg caggataccc ggcgaatatt atcaaagata aggcgaaagt aatgcaggac
     1201 ttttagactt tgtatcgaag attaaatata acaacagtcg gaggtctgga tgaaatggct
     1261 gaaaaaagga agctcagaat ccgccatcta ttgcttttat ttttcttaat ttacataatg
     1321 tttaccttag taagccagca gttcaaaatc gcggaacttg cccggaaaga tgcggagctg
     1381 gaagccgaaa aaactgcatt gctgcaggaa agggaggaac tgaaaaaaga gataagcctc
     1441 cttcacaccg acgaatacat agagggaatt gcccgggatg aattgggcct tgtaaagccg
     1501 ggagagtaca taataaaaag ctcgaggagc agcaaaagat aaagggggaa aaattagctc
     1561 aaatggtatt agcagcaaaa atagctaaaa agcttttgtt tattgacgaa ttttaaaagt
     1621 taaagtataa ttgacaatat ctataggaga ttttaaagga ggaatttttt aagtatgcct
     1681 gttgaggtag gccaaattgt agaggggaga ataacaggga ttactagatt tggggctttt
     1741 atggagattg cgcagggggt taacggactt atacacattt ctgaagtggc tgacagcttt
     1801 gtaaaggatg tcaatgatta tctaaaagaa aatgacattg tcaaggctaa agtcatatca
     1861 atttccaaag atggaaaaat aagtctgtct cttcgcaagg ccaacgaaaa tgctaaaaca
     1921 aaacaagtcg atcagagttt tgaggataag ctggccaaat ttttaaaaga cagtgaagag
     1981 cgcatgttgg acattaaaag gtatcatgaa tctaaacgag gatcagggtc ctacggcaga
     2041 aggaaggttc tgtaggggat tctgactcat atactataag aaaatatcca gggaaattcc
     2101 ctggatttta aatttgcgag aggggcaaat tatgagagtt gcggtcattg accttggttc
     2161 caactccatt aggctattag taaaagactt gtgggcatct tccatggcaa ccgtgcacaa
     2221 agagctggcg accacgcgct tgggccgagg tgttgtaaga agctcggagc ttgaccggca
     2281 gtctatggca gataccctat cggttattgc tgagtttatc agcagggcaa attcaatggg
     2341 aaccgagcgg atcttagcct ttggcaccag tgcccttcgg gaggccaaaa acagcagcgg
     2401 ttttataaag aaggtaaatg agctgggtct tgatgtagat atattgtcag gtgaagaaga
     2461 ggccctcctt aattttaaag gggcaagggc agggctaggc ctttcggaaa ctaccctggc
     2521 aatagatatc ggtggtggtt ccacggagct tatcttgggc aaggagcgga tacaaaaaag
     2581 cgcaagccta cccgtagggg cggtgcgctt tactgagctt tattttaatt cggagccgcc
     2641 aaaccctaag gatgtgaagg cggctgggga tgaaatcttc cgacgctgcc ttgactttgc
     2701 aagattttat aatcggacaa agagcggaca cgcaattgtt gccgttggcg tagggggcac
     2761 cctgacaacc ctggctgcca tggaccaaga acttgtgatc tatgatccgg aaaaaataca
     2821 cggctacgcc ctaaaaaagc tatcaataga tgatatttat actaaactat taacccttac
     2881 attagatgaa aagcggaagc tccccggcct tatgccgcag cgggctgata ttatcaccgc
     2941 agggactttt atcgccaaga ccatcatgga gcaccttgaa atatcggaaa tcattataag
     3001 cgaaacggac ataatggaag gctaccttct ctacaagtta gcttaaaaaa atcagaaaga
     3061 aatttagcct aaaatgttga cttagttgaa aaaatgtggt actatattaa attgcataat
     3121 cgtaagcaaa aatcgtgcca ctttttttcg tttataagcc tgaaatacag ttttaccctg
     3181 taaaaagcgc taggattaat cctttgattt atattttacc atgattatca tgattttaaa
     3241 acatcctaaa ttgaatattg tttcctatat taggagatag tataatagat attcttgtcc
     3301 aaaaaccaaa agtaaggcag catgtccgct tttggctatt tttacttata ttaaggggtg
     3361 agggaaacgt ggttcaacct attaccatag gcaatcgcac ccggtggaat ttttccaaaa
     3421 tcgacgaagt catgggtatg ccgaacctaa tcgaaattca gaaggattcg taccagtggt
     3481 ttttgaacga aggcatcagg gaagttttca atgacatctc cccaattgag gattttaccg
     3541 gtaacttggt gttggaattt attgagcata aactcgaatc aacgccaaag tactcggaag
     3601 atgaggcacg ggagagggat gtgacttacg cagttcccct aaaggtcaag gttcgattga
     3661 taaacaagga gaccggagaa gtgaaggaac aggaggtctt catgggagac ttcccgctga
     3721 tgaccgaaaa cggcaccttt ataattaacg gcgccgaaag ggtcatagta agccagcttg
     3781 tacgctctcc gggagtttat tttgacagcc atcgagataa aaacggccgg gagctttttt
     3841 ccggaaccgt aatccccaac aggggagcct ggctggaaat ggagatggac tccaatgatg
     3901 tcttgtacgt caggatagat agaacccgca agctgcccat aacagtgctg cttcgagctt
     3961 tagggtacgg gacaaatgcc cagatcctcg aactcatggg cgaagatgaa aagattttgc
     4021 gcaccctgga aaaggacaat acggaaaatg aaaatgaagc cctcatagaa atatacaaaa
     4081 gacttcgtcc aggtgaaccg cccacggttg aaaatgcaag gtcgcttttg gaatccctgt
     4141 tttttgataa caagaggtac gatttggccc atgtgggccg ttataagttc aataaaaaac
     4201 taaggctcag ggaccgcatt gtcagcaaag taagtgcgga agatgtggaa aatcccgaga
     4261 cgggcgagct agtcctgaaa aaaggcgaaa aaatcagccg caagacggca gagctcctcg
     4321 aatcccttaa gataaaagag cttaaaatca tcatgccgga agacagtcag gaagcaagac
     4381 tgcgtgctga ttatagccca aaggtagcaa aggttataag caacggatat tccgaggaac
     4441 ccggtcctga aggcaacagg acggaaaaac acattacagt agaagatatt gttgcagctg
     4501 ttaactactt tgttaacctc ccctataaaa tcggcaaaat tgatgacatc gaccacctcg
     4561 gaaaccggcg ccttcgctcg gtaggtgaac tcctgcagaa tcaatttcgt ataggcctgg
     4621 ccaggatgga acgggtcatt aaagagcgga tgaccattca ggacgtggat acgattaccc
     4681 cacagggcct tataaatatt cggcccgtcg tagcagctgt caaggaattt ttcggcagca
     4741 gccagctctc ccagttcatg gatcagacca accccttggc cgagttgacc cataaaagaa
     4801 gactcagcgc cttaggcccc ggcggtttga gccgcgacag ggcaggtttt gaagtccgtg
     4861 acgtccacca ttcccactac ggccgcatgt gtcccataga aacacctgaa ggtcccaaca
     4921 tcggcttgat cgggtccttg agtacctatg cgagagtaaa cgaatacgga tttattgaga
     4981 ctccctatcg caaagtggat aaggagcgag gcgttgttac cgatgaaata gtgtatctaa
     5041 ctgccgacga agaggacgaa tacaccatag cccaggccaa cgcacctctt gatgaagagg
     5101 gaaggttcat acaaaagagg gtcgttgccc gctatgccga tgaaattata gttgtcccta
     5161 tagaagatgt ggattacatg g
//
LOCUS       DAONUO010000135         5120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11846_length_5120_cov_3.076604, whole genome shotgun sequence.
ACCESSION   DAONUO010000135 DAONUO010000000
VERSION     DAONUO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11846_length_5120_cov_3.076604"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..428)
                     /locus_tag="PK768_05970"
     CDS             complement(63..428)
                     /locus_tag="PK768_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQA60552.1"
                     /translation="MLVAISSKGNSMESMMDPRFGRAEYFIIADTDTMEFEVLENTAA
                     VSGGGAGITSGQLMIDKDVQAVITGNIGPNAMSVLAAANIEIYRGVSASVKENVEKLN
                     KGLLEEISKTVPPHSGING"
     gene            complement(442..1311)
                     /locus_tag="PK768_05975"
     CDS             complement(442..1311)
                     /locus_tag="PK768_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA60553.1"
                     /translation="MKQIVFISGKGGTGKSTLVSSLSLLVQNKMLADCDVEAPNLHIL
                     LQGEILKKRDFFGAKEAVIDSSACIKCGLCRKTCRFDAISEEFEIKPMKCEGCGACTL
                     VCPQNAIKLEEVKTGETYVSNTSRGTFSHALLDIGAEGSGRLVTEVRKNVNAYKKDEE
                     WLLIDGSPGIGCVVIASITGTDAVVVVSEPTKSGQSDLERVLGVAKHFGVPAFVCINK
                     YNLNLKVTSEIEEYCENNGYPVIGKIPFEPSIVKALQEFKTPIEAGNKVITNEIKNLW
                     ARLVDEVENLKKA"
     gene            complement(1308..2138)
                     /locus_tag="PK768_05980"
     CDS             complement(1308..2138)
                     /locus_tag="PK768_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA60554.1"
                     /translation="MKLAVLSGKGGTGKTTVSASLAAVVPSCQYIDCDVEEPNGAIFL
                     KPELKEKSPVKVLVPEIDMTKCNFCGLCAKACQFNAIAVVKDKVLVFLEICHHCGACK
                     IACPNDAILEVERTIGVVEANEDGTFLQGKLDVGEPISIPIINELKKRIKEDRFVILD
                     CPPGASCAVVNSIEGCDYCVLIAEPTPFGLHDIKIAVQLVKKMGIPFGVVLNKASNDN
                     RSIHHFCQSEGIEILMEIPFSKEIAEGYSKGILPVEINDLWNEKFKKLYEKIEGGAHK
                     "
     gene            complement(2228..2665)
                     /locus_tag="PK768_05985"
     CDS             complement(2228..2665)
                     /locus_tag="PK768_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQA60555.1"
                     /translation="MEPALAEKIRKKQLQWTPQRETVFSVIANSNNKHLTAEEIFLKT
                     KKIMPTIGLATVYRTLELFCEEGILQKFNLPGEAARYEMLAEDNDVHYHYICLGCGKI
                     FEVPIQKETISSQIENDNIRDFIITKISCWYFGYCKDCMKRQG"
     gene            complement(2750..3130)
                     /locus_tag="PK768_05990"
     CDS             complement(2750..3130)
                     /locus_tag="PK768_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HQA60556.1"
                     /translation="MPRGDRRGPAGFGPMTGRGAGFCAGYPVPGYMNPAWRVWPRGAG
                     YGFGPGFGRGRGWRWHAYATGLPYWVRGYPLFDPMAAYPAAELEPKYEAEILAQDAKI
                     LKEQLKQIEARLEELEKEEKEGED"
     gene            complement(3328..3558)
                     /locus_tag="PK768_05995"
     CDS             complement(3328..3558)
                     /locus_tag="PK768_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60557.1"
                     /translation="MIVFGPVPSRRLGRSVGINNMNITAVHPMKKPAVETLLQKCNSN
                     WNVVEKLIDEQKIIKFEYQNETFYMRKLSEQR"
     gene            complement(3571..4434)
                     /locus_tag="PK768_06000"
     CDS             complement(3571..4434)
                     /locus_tag="PK768_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HQA60558.1"
                     /translation="MKLFLDCIPCFQKQVLFTVKDLDDKLKSKILRKVMILLCDTSWE
                     LTPDEIANKAYNLIREETGIADPYKQIKKQSNKMAMELYPKLKTEMKSIRQKDRLYIA
                     ANLAIAGNIIDYGPAFEFDLGKTIKEVLNKKPAINDFDILTDKVMSAESLLYFADNAG
                     EIALDKMFIQEMITIRGKPFNHISFVVKGGPIINDAMIEDAYEVGIDKLPNVVFYKIG
                     NGQEGTGPNRWEDVVKEWIKEHDLVISKGQGNFEGLSENSNIFFMLIAKCPVIAKEIG
                     VNVKDTVIKYN"
     gene            4628..>5120
                     /locus_tag="PK768_06005"
     CDS             4628..>5120
                     /locus_tag="PK768_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA60559.1"
                     /translation="MKFKATVLCENYVIGNTGVIAEHGWSIFFETNHGNFLFDTGQGK
                     AIINNARIFNIDLSSVKAIMLSHHHFDHTGGLLDALSVTGQVDVYAHPELFKESYSVR
                     ENDKKRYIGIPFARPILESYGAQFKFNTTWQEILPGIYLTGEIPRITEFENGDKNLVI
                     QKGN"
BASE COUNT         1426 a         1133 c          779 g         1782 t
ORIGIN      
        1 agtaagtaat taggtgattt acgaatcatt atttccttta agaaaatcta caaatttttc
       61 cgttaaccat tgatccctga atgcggcggg accgtcttac ttatttcttc aagaagtccc
      121 ttattgagct tttcaacatt ctctttaact gatgcagata ctccacggta tatctctata
      181 tttgcagccg ctagaacact catggcattt ggcccaatat tgcctgttat tactgcttga
      241 acatccttat caatcataag ttggcctgag gtaattcctg ctccaccgcc cgagacagcc
      301 gcagtatttt caagtacttc aaactccatt gtatcggtat cagcaatgat aaaatattca
      361 gcccttccaa accgcggatc catcatactt tccatggaat taccctttga tgatatcgct
      421 accaacatag ttttccctcc attatgcttt ttttaggttt tcaacttcat caacaagcct
      481 tgcccacaaa tttttaatct catttgttat tactttattg ccggcctcaa tgggagtttt
      541 aaattcctgt aaagctttaa ctatagatgg ttcaaaaggt atcttaccta taacaggata
      601 tccattgttt tcacagtact cttctatctc tgatgtcact tttagattta aattatattt
      661 atttatacat acaaatgcag gtacaccaaa atgcttagct acgcctaaaa ctctttctag
      721 atcactttga ccggacttcg taggttcaga aaccacaact actgcatccg ttcctgtaat
      781 agaagcaata acaacacagc ctatacctgg agaaccatca atcagtaacc attcttcgtc
      841 ctttttataa gcgtttacat tctttcttac ttctgttaca agtcttcctg atccttctgc
      901 cccaatgtca aggagggcat gggaaaaagt gcctctcgag gtattggaca cataagtttc
      961 gccggttttt acttcttcca attttattgc attttgaggg catactaatg tgcaagctcc
     1021 acaaccttca catttcatgg gttttatttc aaattcttca cttatggcat caaacctgca
     1081 tgtttttctg cataaaccac actttatgca agctgatgaa tcaattacag cttctttagc
     1141 accaaaaaag tctctttttt ttaagatttc accttgcaga agaatatgca agtttggtgc
     1201 ttccacatca cagtcagcca gcattttgtt ttgaacaagc aaactaagtg atgatactag
     1261 ggtgcttttt cctgttccac cttttccgct tataaataca atctgcttca cttatgagca
     1321 cccccttcaa ttttttcata cagcttttta aatttttcgt tccataaatc attaatttca
     1381 actgggagta taccttttga ataaccttcg gcaatttctt tggaaaaagg aatttccatt
     1441 aaaatttcaa taccttcact ttgacaaaaa tggtgtatcg acctattatc attagatgct
     1501 ttatttaaca caacgccaaa aggtattccc atctttttta caagctgtac cgcaatttta
     1561 atatcatgaa ggccaaaggg tgtaggctct gctattaata cacaataatc acagccttcg
     1621 atggaattta ccaccgcaca ggatgcccct ggcgggcaat ccaatataac gaacctgtcc
     1681 tctttgatgc gcttttttaa ttcatttata ataggtatgc taataggctc acctacatcg
     1741 agtttcccct ggagaaatgt gccgtcctca tttgcctcaa ctacaccgat ggtcctctcc
     1801 acttctagga tcgcatcatt gggacaagct attttgcacg ccccgcaatg atggcagatt
     1861 tcaagaaata caaggacttt atccttcact acagctatag cattaaattg acatgcctta
     1921 gcacagagac cacaaaaatt gcatttggtc atatctattt ccgggactaa tacttttacc
     1981 ggcgattttt cttttaactc gggctttaaa aagatagctc cattaggttc ttccacatcg
     2041 caatctatgt actgacatga gggaacaact gctgccaaac ttgcagaaac ggttgttttg
     2101 cctgtccctc cctttccgct caaaaccgct agcttcattc tttacctcct aacaagacca
     2161 agcttaattt gaatattact caaatcatgg ccagtgtagc caaaagcttg ttttggaaat
     2221 ccttaattca tccttgtctt ttcatacagt ctttgcaata tccaaagtac caacatgata
     2281 ttttagttat aataaaatct ctaatattat cattttctat ttgagaggat atggtttctt
     2341 tttgaatagg tacttcaaaa atctttccac atcccaagca tatatagtga tagtgaacat
     2401 cgttgtcttc tgctaacatt tcatatcttg cagcttctcc tggtaaatta aatttttgta
     2461 aaataccctc ttcgcaaaac agttctaatg tgcgatatac tgtagctaat ccaatggttg
     2521 gcataatttt ttttgtcttt aaaaatattt cttctgccgt tagatgttta ttattactat
     2581 ttgctataac tgaaaatact gtttctcgtt gaggtgtcca ttgcagttgt tttttcctta
     2641 ttttttcggc tagagcaggt tccatttctt caccaccttt tgcctgggaa attctttata
     2701 aaaatttggc ggaagcaaag ccgcctgcct ccgccaagat ctatcttctt tagtcttcgc
     2761 cctctttttc ttccttttcc agttcttcca atctagcctc tatttgttta agttgctctt
     2821 ttagaatttt tgcatcctgt gcaagaatct cagcctcata cttcggttca agctcggcag
     2881 ccggatatgc cgccatggga tcaaaaaggg gatatcccct tacccagtat ggtaatccag
     2941 tggcatatgc gtgccacctc cagcctctgc cccggccaaa gcccggccca aagccgtaac
     3001 ctgctcccct tggccagacc ctccaggcag ggttcatgta gcctggcacg gggtatcctg
     3061 cacaaaagcc tgcccctcta ccggtcattg gcccaaaacc tgcgggtcct cttctatcac
     3121 ctctgggcat gtctccacct ccttccgagg attatttctt taaaagatta tggtcatttg
     3181 ttcataactt tgattttatt ataatactat tatggactta tgtcaataat gatattcttt
     3241 tatttttata tataatccat atggatatag ttctcaattt ttatgaatct cataatgtaa
     3301 caaaaatttc tgttttttgt tatattgtta cctttgctct gacagtttcc tcatataaaa
     3361 tgtttcattt tggtattcaa attttataat cttttgctca tcaatgagct tttctactac
     3421 attccagttg ctgttacatt tttgcagcaa tgtctcaact gccggttttt tcattggatg
     3481 cactgccgta atattcatgt tgtttatccc aacacttctt cccaatcgcc ttgatggaac
     3541 aggaccaaat actatcaatt ggcttcctcc tcaattatat ttaataactg tgtccttcac
     3601 attaacaccg atttcttttg ctataacagg gcatttggct attaacataa aaaatatatt
     3661 gctgttttca cttaaccctt caaaatttcc ctgacctttt gaaataacta aatcatgttc
     3721 ttttatccac tcttttacaa catcttccca tcgattaggg ccggtgcctt cctgaccgtt
     3781 ccctatttta taaaaaacta cattaggaag cttgtctatt cctacctcat aagcatcttc
     3841 aatcatagca tcattaatta tagggccgcc tttcactaca aatgaaatat gattgaaagg
     3901 cttacctcta attgttatca tttcttgaat aaacatttta tctagtgcta tttctccggc
     3961 attgtcagca aaatacaaaa ggctctctgc agacataact ttgtctgtca atatatcaaa
     4021 atcatttatc gccggctttt tatttaatac ctcttttatt gtttttccta aatcaaactc
     4081 aaaggctgga ccataatcaa taatattccc tgctatagcc aaatttgccg caatgtaaag
     4141 ccggtccttt tgccttatac ttttcatctc tgtcttgagt tttggataca attccatagc
     4201 catcttatta ctctgcttct taatttgttt gtacggatcc gctattcccg tttcttcccg
     4261 aatgagattg tatgccttgt tggcaatttc atccggtgta agttcccaac tagtgtcgca
     4321 caaaagaatc ataacttttc tcagtatctt tgatttaagc ttatcatcca gatcttttac
     4381 tgtaaacaaa acctgttttt gaaagcaggg tatacagtct aaaaatagtt tcatttaatt
     4441 ttgctccctt cattcttcct tttcactaac ctgatttagt gattataatt atattattgt
     4501 tgtgaacata tgtcaattac ggccagaatt gatttacata atcagaaatg ctattatata
     4561 aaaaagttaa tgaacttatg accaaaactt tttatagttt ttaaaacaat tgaaaaggag
     4621 tatggatatg aaatttaaag ccacggttct ttgtgaaaat tatgtgattg gcaatacagg
     4681 tgttattgct gaacatggat ggtcaatatt ttttgaaaca aatcacggga atttcctctt
     4741 cgatactggc cagggcaagg ctatcattaa caatgcacgg atatttaata tcgacctttc
     4801 ctcagttaag gccatcatgc tcagccatca tcacttcgac cacaccggag gtctccttga
     4861 tgccttatca gttacagggc aggtagatgt atatgcccat cctgaacttt tcaaggaaag
     4921 ttattcagtt agggaaaatg acaaaaaaag atatatagga atcccctttg ccaggcctat
     4981 attagagagt tatggagccc aatttaaatt caatactact tggcaggaaa tcttgccggg
     5041 aatttattta acaggagaaa taccgaggat cactgaattt gaaaatggag acaaaaatct
     5101 tgttattcaa aaaggcaatc
//
LOCUS       DAONUO010000136         5110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11885_length_5110_cov_4.710188, whole genome shotgun sequence.
ACCESSION   DAONUO010000136 DAONUO010000000
VERSION     DAONUO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11885_length_5110_cov_4.710188"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..973)
                     /gene="fliG"
                     /locus_tag="PK768_06010"
     CDS             complement(101..973)
                     /gene="fliG"
                     /locus_tag="PK768_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HQA60560.1"
                     /translation="MARNKLSGRQKAAILIVSLGPEVAANIYRHLREEDIEQLTLEIA
                     NLAKVSNEQKNEILEEFYQMLVAQNYITEGGIEYAKVVLEKALGTQKAIEIINRLTSS
                     LQVRPFDFIRKADPSQLMNFLQNENAQTIALVMTYLTPEQSASLLSSLPPEKQVDIAR
                     RVATMDRTSPEIIMEVERVLERQMASVVTEDYTSAGGIQAVVNILNNVDRGTEKNIIE
                     ALEVDNPELAEEIRRRMFVFEDVLTLDNRSIQRTLREVDNNDLTLALKGASEDVKKRI
                     FENMSKRQAEMIRG"
     gene            complement(984..2543)
                     /gene="fliF"
                     /locus_tag="PK768_06015"
     CDS             complement(984..2543)
                     /gene="fliF"
                     /locus_tag="PK768_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HQA60561.1"
                     /translation="MDYLQEIKHRAIEFWQSRNRAQKVKIIVFAVAIISSLSVMLYII
                     NRPVYVPLYTNLDVKDAAEIAKKLDENNIRYRLEDQGETILVDPEQRYKIRLILAQEG
                     LPKGGSIGFDEVFSQSRLGTTEWERRIQYNQALQGELSRAIEMMKSVERAKVYIVQQE
                     DTLFIEPNAIHEPSAAVFLQLKAGNEMTNEEIMGIINLISYSVKNMKPENVTVVDQYG
                     RTLSNIPLSQEEDAPGTINNQLIIQNSFQNQLQANVQSLLEQIFGPGNVAVRVNAELN
                     FDKKTVQNKLFTPVNEETGEGIVRSIQELREYFSGTGADPVGVPGVDTNTPPGYVQVG
                     TGESEHQRTEVIKNFDINETLETLTVMPGAVNRLTVSVVINRELSESDKESIASMVGN
                     AIGYDPERDQISVEGMEFQNEMARLLADEVARQQREQQRMRIYRIAGIVLVALLAFIA
                     IRTIISKRRKLEEEELAEEMLAMQQAAVSKTEEESLEVKDNEIYRKIERHARRNPEDV
                     AKVIKTWLIED"
     gene            complement(2561..2854)
                     /gene="fliE"
                     /locus_tag="PK768_06020"
     CDS             complement(2561..2854)
                     /gene="fliE"
                     /locus_tag="PK768_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HQA60562.1"
                     /translation="MKIELDNKTMTNHLINSGGAKVQGEKSFGEVLKNALESVNTYQK
                     EYETVLYRHLIGDDVDLHDVVIAGEKARLSLELTLQIRNKAMEAYQEIMRMQI"
     gene            complement(2879..3304)
                     /gene="flgC"
                     /locus_tag="PK768_06025"
     CDS             complement(2879..3304)
                     /gene="flgC"
                     /locus_tag="PK768_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HQA60563.1"
                     /translation="MFDMLDISASGMTAQRLRLDVISNNIANVNTTRTNEGGPYQRER
                     VVFQEIRDRKSFSDYLRESKRAGVRVVSIQKDDAPFKTVYDPTHPDADAEGYVRLPNV
                     NIVTEMVDMISALRSYEANVTAINAAKSMMSKALEISRI"
     gene            complement(3314..3715)
                     /gene="flgB"
                     /locus_tag="PK768_06030"
     CDS             complement(3314..3715)
                     /gene="flgB"
                     /locus_tag="PK768_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HQA60564.1"
                     /translation="MNMFKTIDFMGTLLDVKLKRHELISNNLANVDTPGYKRYDISFE
                     ELLKSKLNKSSIPLATTHDKHIDTKKQLMDVKPVIYREENHSFRNDNNNVDIDKEMVE
                     MVKNTLSYNIISEQISKNLKLLQTAISEGRK"
     gene            complement(3937..4722)
                     /gene="codY"
                     /locus_tag="PK768_06035"
     CDS             complement(3937..4722)
                     /gene="codY"
                     /locus_tag="PK768_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HQA60565.1"
                     /translation="MSKSLLEKTRKINKLLQRSAGYPVDFNDVCKSLGEVLSSNAYVA
                     SRKGKVLGFYLLEDYDCNAVEEDILEDKMFPKEINDKLLDIHETKQNISPNSSDFIFQ
                     NGSEANNADKLITIVPINGGGERLGTLVLSRYKHEFTEEDLVLAEYSATVVGMEILRS
                     KSEEIEEEARKKAVVQLAIGTLSFSELEAVEHIFEELDGNEGLLVASKIADRVGITRS
                     VIVNAFRKFESAGVIESRSLGMKGTYIKILNDKLLEELKKVRS"
     gene            complement(4790..>5110)
                     /locus_tag="PK768_06040"
     CDS             complement(4790..>5110)
                     /locus_tag="PK768_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60566.1"
                     /translation="TLFKLSPFVYFNSLIKQYTALIETEGIKMSFSEDAIDEIVRVAA
                     YVNQQSENIGARRLATVMEKILEDISFTAPELPQKEITIDKNYVNDKLIDIIKDKDLS
                     MYIL"
BASE COUNT         1353 a         1105 c          798 g         1854 t
ORIGIN      
        1 tattaaaagg catacaacgg agcgggtatg ctatatagct ggttttgtat caaaaagatg
       61 gggatacctt taactttaat tatcctacaa caacttgaca ctatcctctt atcatttcag
      121 cttgacgctt agacatgttt tcaaatattc ttttctttac atcctcactt gcacccttca
      181 atgccagtgt taaatcatta ttgtcaactt ctctaagagt acgctgtata gacctattgt
      241 caagagttag aacgtcttca aagacaaaca tccggcggcg aatttcttct gccaattcag
      301 gattatccac ttccaaagct tctatgatat ttttctctgt acctctatcg acattattga
      361 gaatgttaac aaccgcttga ataccacccg cactggtgta gtcttccgtt accaccgaag
      421 ccatctggcg ctctaaaacc ctttccacct ccattataat ttcaggagaa gtcctatcca
      481 tagttgccac acgtcgcgct atatcaactt gcttttccgg tgggagtgag gaaagaagtg
      541 atgcagattg ttccggtgtt aaataggtca taacaagggc tatagtttgc gcattttcat
      601 tctgtaaaaa attcattaat tgagatggat ccgcttttct tataaaatca aaaggcctta
      661 cttgcagcga tgatgtaagt ctattgatga tctcgatagc tttttgggta cctaaggctt
      721 tttccagcac tacttttgcg tattctattc caccttcagt tatgtagttt tgagcaacta
      781 gcatttggta aaactcttct aaaatttcat ttttttgttc gttggaaact ttagctaaat
      841 ttgcaatttc aagcgtcagc tgctcaatat cttcttctct taaatggcga tatatatttg
      901 cagcaacttc gggccctagt gacactatta aaatagcagc cttctgtctt ccgctcaact
      961 tgtttcgtgc cataaatcca cctctaatct tctatgagcc atgtttttat aacttttgcc
     1021 acatcttcag gatttcttcg agcgtgtctt tctatttttc tgtaaatctc gttatccttg
     1081 acttccaatg actcttcttc tgtttttgaa accgcagctt gctgcatagc gagcatttct
     1141 tcggccagtt cttcctcctc caactttctt ctcttgctaa taattgtcct tatggcaata
     1201 aaagccagga gggccactaa aacaattcca gcaatcctat aaattctcat gcgctgttgt
     1261 tcacgctgct ggcgggcaac ttcatcagct aaaagcctgg ccatctcatt ttgaaactcc
     1321 atgccctcta cggaaatttg atctctttct ggatcatagc caattgcatt tccaaccatt
     1381 gatgcaatag attctttatc actctcactc agttcacgat tgataactac ggatacggta
     1441 agcctgttta ctgctccagg cattactgtg agagtttcaa gggtttcatt tatgtcgaaa
     1501 ttcttaatga cctcggttct ttgatgttcc gattctccgg tcccaacctg gacgtaaccg
     1561 ggtggtgtat ttgtatcaac ccccggcact ccgactggat cggcgcctgt accgctaaaa
     1621 tactctctta gctcctggat actccgaacg attccttcac ccgtttcctc attaactgga
     1681 gtgaataatt tgttttgtac cgtcttttta tcaaaattca gttcagcatt aactctaaca
     1741 gctacattgc ctgggccgaa aatctgttct aataaagatt gaacatttgc ttgaagctga
     1801 ttctgaaaac tgttttgaat aattaattga ttgtttatag ttccaggagc atcctcttct
     1861 tgagagagag gtatgtttga aagggtcctg ccatattggt ctaccacagt aacattttct
     1921 ggtttcatat tttttactga ataagatata aggttaataa tgcccattat ttcttcattc
     1981 gtcatttcat tgccagcttt taattgcaaa aatactgcag cagaaggttc atggattgca
     2041 ttgggttcta taaatagggt atcttcttgc tgtacaatat aaactttagc ccgttcaacg
     2101 gatttcatca tttcaattgc tcttgaaagt tctccctgta gtgcttgatt gtattgtatt
     2161 cgtctttccc attcagtggt acctaatctt gattggctga atacctcatc aaagcctata
     2221 gagccacctt taggtaaacc ttcctgggct aatattaacc ttattttgta tctctgttca
     2281 gggtctacca gaatggtttc cccctggtct tcgagtctat atcgaatatt gttttcatca
     2341 agtttcttag caatttctgc tgcatctttt acatcaaggt tggtatataa aggtacatac
     2401 acaggtctat taataatata aagcatcacg cttaagcttg aaatgatcgc aactgcaaaa
     2461 acaatgattt ttactttctg tgccctattt ctactttgcc aaaactctat agctcgatgc
     2521 tttatttctt gcaagtaatc cattgtttac acctgtcctg ttaaatctgc attctcatga
     2581 tttcttggta tgcttccata gccttgtttc gtatttgcaa agtaagctca agggatagtc
     2641 ttgctttctc acctgcaatt acgacatcat gtaaatcaac atcatcgcca atgagatgac
     2701 gataaaggac agtttcatat tctttttgat atgtatttac tgactctaac gcattcttta
     2761 atacttcccc aaatgacttt tccccctgta ctttagctcc cccagaattg atgagatgat
     2821 tagtcatggt tttgttgtca agttctatct tcactgcttt atcccctttc aagcttcatt
     2881 atatcctact tatctcaagt gctttactca tcatgctttt agcagcgtta atagctgtta
     2941 catttgcctc atagcttctt aatgcagata tcatatctac catttctgta acaatattta
     3001 cattgggaag cctgacatat ccctctgcat cagcatcagg atgagtcggg tcatacacag
     3061 ttttaaaagg tgcatcgtct ttttgaattg aaacaaccct cacgccagca cgcttgcttt
     3121 ctctgaggta atcactaaaa gacttccgat cccttatttc ctggaaaact accctttccc
     3181 tttgataagg gcctccttca tttgtccttg tagtgtttac attggcaata ttgttagaaa
     3241 taacatcgag cctaagcctt tgcgccgtca ttccggaagc actaatgtcc aacatatcga
     3301 acattcccac ggactacttc ctcccttcgc tgattgcagt ttggagaagt tttaggtttt
     3361 tacttatttg ctctgatata atattatagg ataaagtgtt tttcaccatt tccaccattt
     3421 ctttatcaat gtctacattg ttattatcgt ttctgaagct gtgattctct tcgcgatata
     3481 ttacgggttt aacatccatt aattgttttt tcgtatcaat atgcttatca tgtgtggttg
     3541 ctaaaggtat gctgctctta tttagtttgc ttttcaataa ctcttcaaaa gatatgtcgt
     3601 atctcttgta accgggagtg tcaacattgg ccagattatt agaaataagt tcatgtctct
     3661 ttagttttac atccaataaa gttcccataa agtcgattgt tttgaacata ttcattcatt
     3721 cacccttttt ttctagccta agccggaatg gaatatattg cttatctata atattattag
     3781 acataaatcg ccaaaatcct tcttcagatt gcaactataa atccaaaatt ttctagttta
     3841 taaataaaaa ggaaggattt gttgtaaata ctatattaga catttatcaa caaaatcctt
     3901 ccaaatatta tttttacatg gatttttaca tccggattac gatcttacct ttttcagttc
     3961 ttccagaagc ttatcattta gaatctttat gtatgtcccc ttcattccaa gggaccgaga
     4021 ttctataact cctgcacttt caaattttct aaaagcattt acaattactg accttgtgat
     4081 acctacccta tcggcaattt tgcttgccac caaaaggcct tcgttgccat ccagttcctc
     4141 aaaaatgtgt tccacagctt ctaactctga aaaagacagg gtgcctattg caagctgaac
     4201 aactgccttt tttctagctt cttcctctat ttcttcactc ttagatctca aaatttccat
     4261 tcctactaca gtagcactgt attcggcaag taccaaatct tcttcggtaa actcatgttt
     4321 atatctagaa agtaccaatg tgccgagtct ctctccgccg ccgttaatgg gaacgattgt
     4381 aatcagttta tctgcattgt ttgcttcgct tccattttgg aaaataaaat cggaagaatt
     4441 tggagagatg ttctgttttg tttcgtgaat gtccagcagt ttgtcgttta tttctttcgg
     4501 aaacattttg tcctctaaga tgtcttcttc aactgcattg caatcataat cttccaacag
     4561 gtaaaagcct agaacttttc cttttcgact ggctacgtat gcatttgatg acaaaacctc
     4621 accaagactt ttacatacat cgttaaaatc tacaggatac cccgcagatc tttgtaagag
     4681 tttgttaatt tttctcgtct tttctaacaa gcttttgctc attttcatat tcctcccatt
     4741 aaactttttt tcaaatttta aaacaaaatg tattattgtt tattgtgttt tacagaatat
     4801 acatgcttag atctttatct tttatgatat ctattagctt atcattaaca taatttttat
     4861 cgatcgttat ttccttttga ggtaattccg gtgcagtaaa tgaaatatct tccaatattt
     4921 tctccataac tgtagcaagc cttcttgctc caatattttc cgattgctgg ttgacatatg
     4981 ctgccactct aactatttca tcaatagcat cttcggaaaa gctcattttt ataccttcag
     5041 tttctatcaa agcagtatat tgtttaatta gcgaattaaa atatacaaat ggcgataatt
     5101 taaatagtgt
//
LOCUS       DAONUO010000137         5107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_11894_length_5107_cov_4.022961, whole genome shotgun sequence.
ACCESSION   DAONUO010000137 DAONUO010000000
VERSION     DAONUO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11894_length_5107_cov_4.022961"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..1664)
                     /locus_tag="PK768_06045"
     CDS             complement(423..1664)
                     /locus_tag="PK768_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQA60567.1"
                     /translation="MKGKIKIIGAIVAVSLLILAVLYQSTKPISAELLEVKPQTIART
                     FAEEGKVIPADKAPVYSLVSGEILKLEVEEGQRVKENDLLAVIDSTELELQLKQMQAE
                     LVSLKGEEADRYKEPLESSIKSQELLVEKANLDLEAIENDFKRIENLYNEGAVTAKEY
                     EDAKNMLETTKLNLRQQEEALALLYESAEPSSGSRQFYAGRADALQTQMELIKYRIER
                     SRITAPAEGVVANLAVAKGDVASPGMKLLDIFTGDDYLVEVFVLAESAASVTEGMKVK
                     LTLDRKGEDITFDGVVEKIAPSAVEIISALGIEESRVKVTVKPEYPGTLRVFPGSTLD
                     VEFTTDAREGVLAVPKTALFPYENGEALWKVIDGKAVIQPVETGFENDSHVVITKGLA
                     PGDLIILNPQLEGLKEGKKIS"
     gene            complement(1838..4201)
                     /locus_tag="PK768_06050"
     CDS             complement(1838..4201)
                     /locus_tag="PK768_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQA60568.1"
                     /translation="MLFRKMIRDIRENRGAYLACMVIITLGLMIFTAFSTMAGTLQTS
                     QQSFYEGQNFADGFAQVQAIPKDEISKLEDVEGIKEIQGRLVKEVMVYAPESKENIYL
                     RLVSVDNDAQNPINEPLLKTGFPLEDNLLNIWVDNQFFEANNYSLNDEIEVIASGRKH
                     KLSISGVASSPEFVYALRTGADLFPTPETFGIAFMPLTSMEKLFPDQKAYNDIVFTLR
                     PGADFEKVKTELEIKLKPYGLESLISRDEQTSHLLLSEELKGLVSMSKIMPIVFLTIA
                     AVIMYITLKRLIEQQRIQIGILKAEGYTTQEIMLHYMSYAVIIGLVGGILGVISGGIL
                     SYPLSSIFQVFFNLPGLEGRFSPSLAISGIILAVSFSLLAGFQGSRNVLALQPAEAMR
                     PPAPVQGKRVLLERIGIIWKRLTVQQMMAVRNMSRNKGRSFFIFLGMMVCFAISSFTW
                     SMNDMIQKMMYDQYEKVEVYDVKLSLARPMEEKSVVRELAAFPGVSRVEAMAEIPVTL
                     KHKWHEKDVVILGLPENCSLYNILDDEYNKLAPPKNGLLISERLAKLLDAEPGTRLTV
                     ETAMRADDKDGTLQVLGIIPQYSGTNAYMEIGSLQDFLRQRGLITSAMLKINKIDIPS
                     LREKYVHTDVISSIDERSQRLEKFKDMMGSYGSMIYIYAMISVVIGFAIIYSSSIITL
                     SERSRELASMMVLGMTPQEVLSVVTFEQWCLALPAMLLGIPLARVLLIGMSSAMSTDM
                     YTIPERITLTALLLAFLVTTLSVWLAQKVAARKIRTLSLVEVLKARE"
     gene            complement(4201..4905)
                     /locus_tag="PK768_06055"
     CDS             complement(4201..4905)
                     /locus_tag="PK768_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60569.1"
                     /translation="METIIMAEDLSKTYIMGEVEVRALKEANFELMEGELVVVLGPSG
                     SGKSTLLNIIGGMDKPTGGRLYYKGRPLHEADQRQLTLYRRNEVGFVFQFYNLMPNLT
                     ALENINLSVQIAQNPLSAEKLLEQVGLADRADHFPSQLSGGEQQRVALARALAKNPKM
                     LLCDEPTGALDYSTSIQVLSLLKSFCDMYSKTVVIITHNTAIAQMAHRIFYLKDGRLE
                     RIEKNENPLPPEKVTW"
BASE COUNT         1280 a         1241 c         1065 g         1521 t
ORIGIN      
        1 atattgcctt tgtgaaggtt cacgatgaaa aacatttaac ggtcgggttc ccacagagag
       61 tagtaacctg ggaagaactg cttgacaggt tacaagttaa gctgtgggtg tatccagacc
      121 gtttagaggt tcggggaatt gtcccgattg aagatatagc gaatcctaaa tgctgttaat
      181 taataaaaat ataattgtac tcacgcgagt aattcctact tatttagttg gttttgtccg
      241 tactatatac tggttcatgg acaatgtcaa tacaatttta agaaatatga cgagcaaaac
      301 tttcctcgtc agagatgtga attttcagaa atatggcaag cgaagcgtcc cgttgtcaga
      361 ccatttgtca tgtgtgagtt ttcagaaatc tggcaaacga aacgtccccc tggcaggtct
      421 tgtcaggaga tttttttgcc ttctttgagg ccttctagct gtgggttcag gataattaag
      481 tctccgggag caaggccctt ggttatgacc acgtggctat cgttttcaaa gccggtttca
      541 acgggctgta ttacagcttt gccatctata actttccaca aggcttcgcc attttcatat
      601 gggaataggg cagttttggg cacggcaagg acaccttccc ttgcatcggt tgtaaactca
      661 acatccaatg tgcttcccgg aaacacccga agtgtgccag gatattccgg ttttaccgtg
      721 accttgaccc ggctttcttc aatacccaaa gccgatatta tttcaacggc agaaggtgca
      781 atcttttcca ccactccatc aaaagtaatg tcttcgccct tcctatcaag agtcagtttt
      841 actttcatgc cttccgtgac acttgccgca gattctgcca ggacaaagac ctccaccagg
      901 tagtcgtccc ctgtaaaaat gtccagcaat ttcataccgg gacttgccac atcgcccttt
      961 gccacggcaa gattagccac tactccctcg gccggcgcag ttatgcggct cctttctatt
     1021 cggtacttaa taagttccat ttgagtttgc agggcatcgg ctcttccagc gtagaactgc
     1081 cgagaaccgc tacttggttc agctgattca taaaggagtg caagagcctc ttcttgctgc
     1141 ctgaggttga gcttggtggt ttccagcatg tttttcgcat cttcgtattc ctttgcggta
     1201 acagcgcctt cattgtaaag attttctatt ctcttaaagt cattttcaat ggcctcaagg
     1261 tcgagattag ccttttccac taagagctcc tggctcttta tacttgactc taaaggttct
     1321 ttgtacctat ccgcctcttc ccctttaagg cttaccagtt ctgcctgcat ctgctttagc
     1381 tgcaactcta attccgtaga atctattacg gccagcaaat cattttcttt tacccgctga
     1441 ccttcttcca cctcaagttt caggatctcg ccgcttacga ggctgtacac gggagcttta
     1501 tctgccggaa ttaccttccc ctcttctgca aatgttctgg ctatagtctg gggcttgact
     1561 tcaagcagct cggcactaat tggtttagta gattgataca aaacagccaa aatcagcagt
     1621 gaaactgcaa ctattgcccc gataattttt attttcccct tcacggtcct cacccattcc
     1681 tctctttttc ttctcttact agttttgatt gtgtctgttt aaacagccgg cggtcaaaga
     1741 ccgcccttac ataatacaaa ggtatcatga gcggcacgca agaaataacc taacaatata
     1801 gtggtctcca ggcacattta aaatatgaac gcataaatta ctcccgtgcc ttcaatactt
     1861 cgactaaact caaagtcctg attttccttg ctgcaacctt ttgggctagc cataccgata
     1921 aagtggtcac caaaaaagcc agcagcaaag ccgtcaaagt tatccgttca ggaatcgtat
     1981 acatgtcggt gctcatggct gaagacatcc ctatcagcaa tactcttgcc aaagggatac
     2041 ccagtagcat agctggcagc gctaaacacc actgttcaaa ggtgactacc gacagcactt
     2101 cctggggcgt catgcccagg accatcatgg aagccaattc ccggcttctc tccgacaggg
     2161 taatgatgga agaactataa attatggcaa agcctatgac tacactaatc atggcataaa
     2221 tatatatcat gctaccatag gagcccatca tgtccttgaa tttttcaagt cgctgactcc
     2281 tctcatcaat agaacttatc acatcggtgt gcacgtattt ttcacgaagg gaaggaatgt
     2341 caattttgtt gatcttcagc atagccgatg ttataagacc tctttgtcgc aagaaatctt
     2401 gaagggaacc tatttccatg tatgcgtttg tgcccgagta ctggggaatg attccaagca
     2461 cctgcaaggt cccgtcttta tcgtccgctc tcatggcagt ttctacggtt agccttgtgc
     2521 ccggctctgc atcaaggagc tttgccagtc gctctgatat aagcagcccg tttttgggcg
     2581 gtgcaagctt gttatactca tcgtccaata tattgtaaag actgcagttt tcaggaaggc
     2641 caagtatcac cacatccttc tcatgccact tgtgttttaa agtgaccgga atctccgcca
     2701 tggcttctac gcggctcacc cccggaaagg cagccagttc cctgaccaca cttttttctt
     2761 ccatgggcct tgcaaggctt agctttacat cgtacacttc caccttctca tactggtcgt
     2821 acatcatctt ttgtatcata tcattcatgg accaggtaaa actggaaatt gcaaagcaaa
     2881 ccatcatgcc gagaaatata aaaaagctgc gtcccttgtt tcgtgacatg ttgcgaaccg
     2941 ccatcatttg ctgcacggta agtctcttcc atattatgcc aattctttcg agcaagactc
     3001 gcttcccctg caccggtgct ggcggccgca tggcctcagc aggctgtaaa gccaagacat
     3061 tcctgctgcc ctgaaatcct gccaggagcg aaaaggatac ggccaggatt atgcctgaaa
     3121 tcgcaagaga tggtgaaaat ctgccttcaa gccccggcag gttaaaaaat acctggaaaa
     3181 ttgatgatag cggataggaa agaatccctc cggaaatgac gccaagtatc cctcctacga
     3241 gtccaatgat tacggcatag gacatgtagt gaagcattat ttcttgagta gtatagcctt
     3301 cggcttttaa aattcctatc tgaatccgct gctgttctat aagtcttttg agggtgatat
     3361 acatgattac agcggcgatt gtaagaaata ctatgggcat aatctttgac attgatacaa
     3421 ggcccttaag ctcctcgctt aaaagcaggt ggcttgtctg ttcatccctg gaaatcaggc
     3481 tctcaagtcc atacggcttt agttttattt caagctcggt tttgactttt tcaaagtcag
     3541 cgccgggcct aagggtaaac actatgtcat tgtaggcttt ttggtcggga aaaagttttt
     3601 ccatgctagt taaaggcata aaagcgatgc cgaaagtctc gggtgtggga aataaatctg
     3661 ctcccgtgcg aagggcgtat acaaactccg gactgctggc cacccctgaa attgaaagct
     3721 tatgctttct acctgacgca attacttcta tttcatcatt taggctataa ttgttggctt
     3781 caaagaactg attatctacc caaatattta gcaggttatc ttcaagggga aaacccgttt
     3841 tgagtagggg ttcatttatg gggttttgcg cgtcattatc caccgatacc aggcgcaagt
     3901 atatattttc cttgctttca ggcgcatata ccatcacttc ttttacgagg cggccctgga
     3961 tttctttaat tccctctaca tcttctagct tgcttatctc atcttttggt atagcctgta
     4021 cttgggcaaa tccatctgca aaattctggc cttcataaaa gctttgctgg gaagtctgca
     4081 aagtccctgc cattgttgaa aaggctgtga atatcataag tccaagtgtt atgatgacca
     4141 tgcaggccag gtatgccccc cggttttccc taatgtccct tatcatcttg cgaaacagca
     4201 ttaccaggtc accttctccg gcggcagtgg attttcattc ttttcaattc tttctaacct
     4261 gccgtctttc aaataaaata tccggtgggc catctgggct atggcggtgt tgtgggtaat
     4321 gatgacaacc gtctttgagt acatatcaca aaagctttta agcaagctta aaacctggat
     4381 gctggtgctg tagtccaagg ccccggtcgg ttcgtcgcaa aggagcatct tgggattttt
     4441 tgcaagggcc ctggccaggg ccaccctctg ctgctctcct cctgacagct gtgatggaaa
     4501 atggtcagct ctatctgcaa ggcccacctg ctccaataat ttttctgcag agagggggtt
     4561 ttgggcgatc tgtaccgaaa gattgatgtt ctccagggcc gtgagatttg gcatcaaatt
     4621 ataaaactga aagacaaaac ccacttcatt tcggcggtac aaagtcagct gtctttggtc
     4681 agcttcatgt aatggccttc ccttgtagta cagtcttccg ccggtgggtt tatccattcc
     4741 tccgatgata ttaagtaagg tacttttgcc ggacccgctg gggcccagca caacaaccag
     4801 ttccccttcc atcagctcaa agtttgcttc ttttaatgct ctaacttcaa cttcgcccat
     4861 tatgtatgtc ttgctcagat cttcggccat tattatcgtt tccaagcaca gccctccctt
     4921 cgatatattc tactactcat atttatgaaa tatttgcatc gttttatttc ataatatcaa
     4981 aaatacaaag ctctgacaat gagttttgaa atatttttgt tgatttattt cattatttct
     5041 tgtattatat gtacgaggtg catgtatatg aacgggtttg agcgcaggac catgcgcaag
     5101 aaaaagg
//
LOCUS       DAONUO010000138         5049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12029_length_5049_cov_3.841410, whole genome shotgun sequence.
ACCESSION   DAONUO010000138 DAONUO010000000
VERSION     DAONUO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12029_length_5049_cov_3.841410"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /gene="pepF"
                     /locus_tag="PK768_06060"
     CDS             <1..1662
                     /gene="pepF"
                     /locus_tag="PK768_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HQA60570.1"
                     /translation="EYRGNIDTAEKLLGLLKLKDEETMKVDRLFTYARMRRDEDNSLA
                     KYQALSDRAMSLVVELSSRTSFIEPEILAIPEEKLRTFLGQNKELKLYEHYLENLFRM
                     KAHILDAEKERMLAEVSEILQSPGDIFKMLDNADIRFPKIKDEDGNEVELTKGRYITF
                     MESENREVRKAAFEALYNTYKSMINTLSSTTAANVKANVFIKNQRKFKSSLEASLFAD
                     KVPVEVYENLIDTVHQRMDLMHRYVSIRKKALGLDELHMYDLYVPLVKGYDKTVTYDE
                     AKQMVIEGLAPMGDEYISILKEGFNSGWIDVYENRGKTGGAYSWGTYGTHPYVLLNFQ
                     GKLNDVFTIAHEMGHAIHTYYSFKNQPYIYSNYTIFVAEVASTCNEAILINYLLEKAS
                     SREEKMYLLNHFMEQFRATLYRQTMFAEFEKIIHEKAEAGEPLNAEVLQEIYHKLNED
                     YYGPDIYIDSLIDYEWARIPHFYSSFYVYKYATGFSSAIAISKDIMENGMPAVNAYKE
                     FLSSGSSDYSLELLKKLGVDLTTPKPIQDALDVFEKLLDEFEKLL"
     gene            complement(1762..2196)
                     /gene="aroQ"
                     /locus_tag="PK768_06065"
     CDS             complement(1762..2196)
                     /gene="aroQ"
                     /locus_tag="PK768_06065"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQA60571.1"
                     /translation="MKILIINGPNINLLGLRETHIYGKMDYGKICALLHEKSKELDVS
                     LDIVQSNIEGEIINFIHGAIGEYDGIIINPGAYTHYSIAIYDALKAVQIPAVEVHLSN
                     VHAREDFRKKSVTAGACIGQICGFGFYGYILAMHALIHHTKR"
     gene            complement(2193..2717)
                     /locus_tag="PK768_06070"
     CDS             complement(2193..2717)
                     /locus_tag="PK768_06070"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQA60572.1"
                     /translation="MSLRQKGINIVLIGMPGSGKSSVGKILAERLGMDFCDVDEFIEK
                     NAGKSIKDIFDEKGESHFRALERTAVEKLSQKTNMVISTGGGVVLCSQNMVNLKRKGV
                     VFFINRPIEDIVKDIDISTRPLLANDIDRIYQLFRERSSLYKKHSDFEILNLKGIEDT
                     TKQIINVLEDRDLL"
     gene            complement(2714..3523)
                     /gene="aroE"
                     /locus_tag="PK768_06075"
     CDS             complement(2714..3523)
                     /gene="aroE"
                     /locus_tag="PK768_06075"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQA60573.1"
                     /translation="MFKSFGVIGENLSHSLSPIIHDKFFKKTGILGTYNLFEVKREDL
                     KDVVRALKVLGIKGANVTMPYKIDIMEYLDELSPEAEKIGAVNTITIADNKAIGSNTD
                     YFGFGMALEYNGIELKGKVAAVLGTGGASRAVSQHLLDKGIRELIFVSRDPQRAIEKY
                     RDFRIITYKELDSIDGDILVNCTPVGMYPNIDESPVEAAQLKQFKVVVDIVYNPKQTL
                     ILKKAQELGLDTVNGMDMLVCQAAKAEELWNKITIDKNIIDEVLLEVEKEL"
     gene            complement(3526..3822)
                     /locus_tag="PK768_06080"
     CDS             complement(3526..3822)
                     /locus_tag="PK768_06080"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQA60574.1"
                     /translation="MDCLEGLRVQIDNIDSCLVDLFEMRMSIALKIASYKMKNGLPIL
                     NEKREAEVIEKNLAKLKNKGFEAELIEFFKTLMHLSKQVQIREFDKYRIKSKED"
     gene            complement(3785..4909)
                     /gene="aroC"
                     /locus_tag="PK768_06085"
     CDS             complement(3785..4909)
                     /gene="aroC"
                     /locus_tag="PK768_06085"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQA60575.1"
                     /translation="MSSIFGNKFKISIFGESHGKAIGVVLDGLPAGIELDMDFIKGEL
                     SRRQPGKSIFSTPRKEKDDFEILSGYFCGRTTGSPLSAVIFNRDTKSRDYEELKLLPR
                     PGHADYTGFVKYGGFNDYRGGGHFSGRLTAPLVFAGAVAKQVLKMQNIIIASHIKSIH
                     DVCDESFDSINIDEEALKELINSDFPVLDALAGQKMKELILDAKKEKDSVGGIIETIV
                     INLEPGLGSPFFDGVESRMAHALFSIPAVKGIEFGSGFDITRLRGSQANDEFVLEGDR
                     IVTSTNHNGGILGGITTGMPLLFRVAIKPTPSIGISQGTVDIQKMQETTIMVKGRHDP
                     CIVPRAIPAVEAAAAVVILDLLFERDGEHGLFRGFKSADR"
     gene            complement(4887..5036)
                     /locus_tag="PK768_06090"
     CDS             complement(4887..5036)
                     /locus_tag="PK768_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60576.1"
                     /translation="MAMALAVAALRCKNPVIINNSDSVGKSYPNFWNDFRKLGGIIDE
                     FHLRQ"
BASE COUNT         1422 a         1182 c          996 g         1449 t
ORIGIN      
        1 gaatacaggg gcaatatcga tacggcggaa aagctcctgg gactattgaa gctcaaagat
       61 gaagagacca tgaaggtgga taggctcttt acctatgcta ggatgcgcag ggatgaagac
      121 aattccctgg ccaagtacca ggcactgtcc gatagggcca tgagccttgt ggttgagctt
      181 tcaagcagga cttcctttat cgaacccgaa atcctcgcaa ttcctgaaga gaaattgcgg
      241 acatttttgg ggcaaaataa agagctaaag ctttacgaac attatcttga gaacctattc
      301 cgcatgaaag cccatattct cgatgcggaa aaggaaagga tgctggcgga agtcagcgaa
      361 atactccaat cccccggcga catcttcaaa atgctggata atgcagatat acgattccca
      421 aagatcaaag atgaagacgg caatgaagtg gagcttacta aagggcgcta tataacgttt
      481 atggaaagtg aaaacagaga agtgagaaag gcagcctttg aagcccttta caacacgtac
      541 aaatccatga taaatacctt gagttcgaca accgcagcta atgtgaaggc aaatgttttc
      601 ataaaaaatc aaaggaagtt taaatcatcc ctggaagcct ccctctttgc cgacaaggtc
      661 cccgttgaag tctatgaaaa ccttatagac acggtccatc agcgaatgga cctcatgcac
      721 agatatgtaa gtattagaaa aaaggccttg ggcctcgatg aactacacat gtacgattta
      781 tacgtaccac ttgtcaaagg ctacgataaa accgtgacct atgatgaagc aaagcaaatg
      841 gtcatagaag gccttgcgcc aatgggagat gagtacatca gcatattaaa agaagggttt
      901 aattccggct ggatcgacgt ctacgaaaat aggggcaaga ccggaggggc gtactcctgg
      961 ggaacttacg gcacccatcc ctatgttctg cttaacttcc agggcaagct aaatgacgtt
     1021 tttacaattg cccacgagat ggggcatgcc atacacacct attattcttt taaaaaccag
     1081 ccatatattt actcgaacta taccattttc gtagcagaag ttgcctcaac ctgcaatgaa
     1141 gccattctca taaattattt actggagaag gctagctcaa gagaagaaaa gatgtatctc
     1201 ctgaatcact tcatggagca gtttagagct accctttatc gccagaccat gtttgctgaa
     1261 tttgaaaaaa tcatccacga aaaggcagaa gcaggtgagc ccttaaatgc tgaggttcta
     1321 caggagattt accacaagtt aaacgaagat tactacggcc cggatatcta catagactcc
     1381 ctaattgatt acgaatgggc caggataccc cacttttaca gcagctttta cgtatataaa
     1441 tatgccacgg gattttcatc agctatagcc atttcaaagg acataatgga aaacggaatg
     1501 cctgccgtaa atgcgtacaa ggaatttttg tcgagcggaa gctcggatta ctcgctggag
     1561 ctcttgaaaa aattaggtgt ggatttgaca acgcccaagc ccatccagga tgccctcgat
     1621 gtgttcgaaa agttattgga tgagtttgaa aaattgctgt aagaagggaa agtaacttta
     1681 aaaaagccat tcatcacaaa taaagtatgt gatgaatggc taaattttta cggtgctagc
     1741 tagataaaca aagtcaacag ctcatctttt ggtatgatga atcaaggcat gcattgccag
     1801 tatatagccg taaaaaccga aaccgcaaat ctgacctatg cacgctccgg cggtaacgga
     1861 tttctttctg aagtcttccc ttgcatgtac gttgctaaga tggacttcca ccgccgggat
     1921 ttgaactgcc tttaatgcat cgtagatggc tatgctgtag tgggtatatg cacctggatt
     1981 tatgattata ccgtcatatt cacctatcgc gccgtgaatg aaattgataa tttcaccttc
     2041 aatattgctc tggacgatgt ctaaagaaac atcaagttcc tttgacttct catgcagcaa
     2101 agcgcatatt ttcccgtaat ccatcttgcc gtaaatgtga gtttccctca agcccagcag
     2161 gttgatattg gggccgttaa ttatgagtat tttcataaca agtccctgtc ctccagcaca
     2221 tttattattt gttttgtcgt atcctcaatt cctttgaggt ttagaatttc aaaatcggag
     2281 tgttttttat aaagggatga tctctctcga aagagctggt atatcctgtc aatgtcattg
     2341 gcaaggagag gcctggttga tatgtctata tctttaacta tatcctctat ggggcgattt
     2401 ataaaaaaca caacaccctt acgctttaaa ttcaccatgt tctgactaca gagaaccact
     2461 ccaccgcccg ttgatatgac catgtttgtt ttttggctca gtttttcgac ggcagtcctt
     2521 tcaagggcgc gaaaatggct ttcgcctttt tcgtcaaaga tatcttttat gcttttgcct
     2581 gcattttttt caataaattc atccacatcg caaaaatcca taccgagtct ttctgccaag
     2641 atctttccca cagagctctt tccggagccg ggcattccga tcagcacaat gttaatgcct
     2701 ttttgcctca agctcacaac tccttttcaa cctcgaggag aacctcgtct attatatttt
     2761 tatcaatagt aattttattc cacagttcct cagccttagc agcctggcaa accagcatat
     2821 ccatcccgtt tactgtatcc aaacccaatt cttgggcctt ttttaatata agggtttgct
     2881 taggattata tacaatatca actaccactt taaactgctt tagctgagca gcttctacgg
     2941 gactctcgtc aatattggga tacatgccaa cgggagtgca gtttactaaa atatccccat
     3001 caattgaatc aagttcttta tatgtaatta tccgaaagtc cctgtatttc tcaatggctc
     3061 tttggggatc cctgctcaca aagatcaatt ctcttatgcc cttatcgagg aggtgctgtg
     3121 aaactgcccg ggaagcgcct cccgttccca agacagcggc gaccttgccc tttaattcta
     3181 taccgttgta ttccagggcc ataccgaatc caaaataatc cgtattgctt ccgattgcct
     3241 tgttgtcagc aatagttatg gtatttacgg caccaatttt ttcggcttcc ggggacaact
     3301 cgtcaaggta ttccattata tcaatcttat agggcatggt cacgtttgca ccttttattc
     3361 ccaaaacttt caatgccctt accacatcct tcaaatcctc tcgcttaact tcaaagagat
     3421 tatatgtccc taaaataccg gtttttttga aaaacttatc atggataata ggtgataggc
     3481 tgtggctcaa gttttcgcct ataacaccga aacttttgaa cataatcagt cctcctttga
     3541 tttaatccta tatttatcaa actctctgat ttgcacctgc ttgcttaggt gcatcagggt
     3601 tttaaagaac tctatcagtt ctgcttcaaa acccttattt ttcaatttgg caaggttttt
     3661 ctctatgacc tcggcttctc tcttttcgtt gagtatcggc aatccgtttt tcatcttgta
     3721 ggaagcaatc tttaaagcaa tactcattcg catttcaaaa aggtccacca ggcagctgtc
     3781 aatattatct atctgcactc ttaaaccctc taaacagtcc atgctcacca tccctttcaa
     3841 agagtaaatc cagaattacc actgccgctg cagcttcaac agcaggaata gccctcggca
     3901 caatgcaggg atcgtgcctg ccctttacca ttattgtagt ctcctgcatc ttctgtatat
     3961 caacggttcc ctggctaatg ccaatcgatg gggtaggctt tattgcaacc ctgaagagca
     4021 ggggcattcc tgtggtaatg cctcctagga tgccgccatt gtggttggtg gaagttacaa
     4081 ttctatcgcc ttccaacacg aactcatcat ttgcctggga gcctctaagc cttgtaatgt
     4141 caaaacctga tccaaactct atacctttca ccgccggaat ggaaaatagg gcatgggcca
     4201 ttcggctttc aacaccgtca aaaaaggggg aacccaggcc aggctcaagg ttaataacga
     4261 tggtctcaat aatcccaccg acggaatcct tttccttttt tgcatcaagt atcagctctt
     4321 tcatcttttg gcctgccagc gcatccagca ccggaaaatc cgaatttatt agttccttca
     4381 aggcttcttc atcgatattt atgctgtcaa agctttcatc acacacatca tgaatgctct
     4441 ttatgtgact tgctattatt atattttgca tctttaaaac ctgttttgcc acggcccctg
     4501 caaaaactag gggagccgta agcctgccgg aaaaatgacc cccacccctg tagtcattaa
     4561 aacctccata tttgacaaaa cccgtgtagt cagcatgacc gggccttgga agcagtttga
     4621 gttcttcata atcccgtgac ttagtatctc tattaaaaat aacggcgctg agggggctgc
     4681 cggtagtcct gccacaaaaa tacccgctca gtatttcaaa gtcatctttt tcctttctag
     4741 gcgtcgaaaa tatgctcttt cccggctgcc ttcttgaaag ttcccctttt atgaaatcca
     4801 tatcaagctc tatgccggcc ggcaagccgt ccaagactac accaatggcc ttgccgtgtg
     4861 actcaccaaa aatagagatt ttaaacttat tgccgaagat ggaactcatc gattatacct
     4921 ccgagctttc taaagtcatt ccaaaaattg gggtaggatt tacccacgga atcactgtta
     4981 ttgataataa cggggttttt gcacctaagt gccgccacag cgagggccat ggctattctg
     5041 tggtcattc
//
LOCUS       DAONUO010000139         5011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12156_length_5011_cov_3.804278, whole genome shotgun sequence.
ACCESSION   DAONUO010000139 DAONUO010000000
VERSION     DAONUO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12156_length_5011_cov_3.804278"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="PK768_06095"
     CDS             <1..1345
                     /locus_tag="PK768_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HQA60577.1"
                     /translation="DSQIEFPVVYASAREGFAKLNLEDPSRDLEPLFKTIIDYVPAPE
                     GDSEAPLQLLITTLDYDDYVGRIAIGRIFRGTTLSGNEYALCKKDGTVEKVKVSNLFA
                     FNGLKRERISSASIGEIIAITGIEDIDIGETIADIDNPEPVPYVSIDEPTLSMIFSAN
                     TSPFAGQEGEYVTSRHLRERLLREARSNVSLRVEETDSPDSFKVSGRGELHLSVLIET
                     MRREGYEFQVSKPTVIIKEINGRKMEPMESVTIDIPETYLGTVMEKMGARHGQLVNMQ
                     NIGSNSVRMEFNIPTRGLLGYRSEFITDTKGEGILNSVFAGYIPYTGDIPTRNRGSLV
                     AFETGESSTYGLYNAQERGTLFIGPQVKVYEGMIVGENSRPQDLDINVCKKKHVTNLR
                     SSTAEEALRLIPPKEMTLEQCLEFIKEDELLEVTPKSLRMRKAILSRQNRQKVKK"
     gene            1452..1631
                     /locus_tag="PK768_06100"
     CDS             1452..1631
                     /locus_tag="PK768_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60578.1"
                     /translation="MAKKKIQRGSRNKLIPQQQRSKADLIDDSSPENYELSEEFAREN
                     PMKTAPNKEKKKKDK"
     gene            1653..1949
                     /locus_tag="PK768_06105"
     CDS             1653..1949
                     /locus_tag="PK768_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60579.1"
                     /translation="MFDFIRETRAVKEGLYNIKLEIPEDIYKSIYEDLNEDKAKDILV
                     EYLKYRQDDARISDIKIEQNKKAHMVNIYANLHYLGNEKSEQEAYTENNINFVK"
     gene            2016..2456
                     /locus_tag="PK768_06110"
     CDS             2016..2456
                     /locus_tag="PK768_06110"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQA60580.1"
                     /translation="MALQTFNAKVEWKGGVVAEATARGFKVTMDEPEELGGTNTAMNP
                     VEMLLCALGGCMSIVASVFAPKFRVELKGLSIEIEGDLDPDGFTGKNPDVRKGFSDIR
                     YKMHIVSDSPEANIEKLHKFITTHCPVKDTLEGVQVSGSYVIEK"
     gene            complement(2775..3584)
                     /locus_tag="PK768_06115"
     CDS             complement(2775..3584)
                     /locus_tag="PK768_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60581.1"
                     /translation="MFKKNIVALIAAMLIVQIFSGIALAEDNSTYVYFEKDVIPLSIN
                     VDLFYTYRPFYSAQKIHRDFDNDGVYEIAVIYNGTHGYKYLTVYKIQGKRMVQMFSGK
                     GVAIRVNSNGFSISNVKYDGRYFYETYTYTWSNGKFLRTGYAKTYIKEGSFDYEKPIK
                     DRPIISKDERILTVNSLLNARMHGKYDEVMNYLSKAYKEKIDSNKLQSIIPYGNVTAI
                     DIFDSQRGDWVVAVIRDIWGNSRVFKFVPVNEKDGYGNIKIDEIVEIPRAN"
     gene            3801..4796
                     /locus_tag="PK768_06120"
     CDS             3801..4796
                     /locus_tag="PK768_06120"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQA60582.1"
                     /translation="MSEGILLFIHKSEANQLLMLLLKNKGAYLSGEDISKRFGITRSA
                     VWKQVNILRDMGYKIKSSPRLGYHLEESPDLLLPEEIWAMSELEVLGSRIYYRSVTES
                     TNNDAKRLAQEGVPDGTLIVAERQQGGRGRMGRMWDSPEGGIWLSVILRPALAPAEAP
                     KLTIMSAVAVAEAIIQLTGISANIKWPNDILIDGKKVCGILTEMSAEMDLINHVIIGI
                     GINVNNDNFPDELKDKSISLKQVKGDKINRVKLLASLLEKLEAYYVKAEREGFDEIFD
                     RWRALCVNLGKRVRIIGKNESFEGTAMDIDSSGALLIKTLNGGIVKVLSGDVSIR"
BASE COUNT         1631 a          908 c         1131 g         1341 t
ORIGIN      
        1 tgattcccaa atagagtttc cggtagtcta tgcctctgcg cgggaaggct ttgccaagct
       61 aaaccttgaa gacccatcgc gggatttaga gcccctgttt aagaccatca tcgattatgt
      121 gccggcgccg gaaggtgata gcgaggctcc cttgcagctt ttaataacta cactagatta
      181 cgatgattat gttggaagaa ttgccatagg gaggatcttt aggggtacca ctctatctgg
      241 caatgaatat gcactgtgta aaaaggacgg gaccgttgaa aaagtaaaag tcagtaacct
      301 ttttgcattt aacggtttga agcgagaacg catatcttct gccagcatcg gtgagataat
      361 agccattacg ggcattgaag acattgacat tggagagacc attgcggata tagacaatcc
      421 cgagcccgtg ccctatgtga gcatagatga acctaccctt tcaatgattt tcagcgcaaa
      481 taccagcccc tttgctggtc aggaagggga atacgtgaca tcgaggcatt tgagggagcg
      541 cttattaaga gaggccagga gcaatgtcag cttaagggtg gaggaaacgg acagtcctga
      601 cagttttaag gtttcagggc gaggagagct gcacctttcc gtattgatcg agactatgcg
      661 aagggaaggt tacgagtttc aggtctcaaa accaacggtg ataataaagg agataaacgg
      721 tagaaagatg gagcccatgg agtcggtgac gattgatatt ccagagacat atttgggcac
      781 ggtcatggag aagatggggg ccagacacgg tcaattggtg aatatgcaaa acatcgggag
      841 caattcggtt agaatggaat tcaacatccc cacccgaggt ctcctaggat acaggtccga
      901 gtttataact gatacaaagg gggagggaat actaaattcg gtatttgccg gctatattcc
      961 ttacactggg gacattccta ctcgaaatag aggctccctg gtggcttttg aaactggaga
     1021 gagtagcact tacggtcttt ataatgccca ggaaagggga acccttttca taggacccca
     1081 ggtgaaggta tatgaaggta tgatagttgg agaaaattca aggccccagg atttggacat
     1141 caatgtttgc aaaaaaaagc atgttactaa cttgcgctca tcaactgccg aagaagcttt
     1201 gaggctgatt cctcccaaag agatgacctt agagcaatgc ctagaattta taaaggaaga
     1261 tgagctcttg gaagtaacgc ccaagagcct tcgcatgaga aaggccattc tttcccgcca
     1321 aaacaggcaa aaagtcaaga aataaattgt caagtattca ctaatttttg gtgaatactt
     1381 ttttgcttta tggatttatt gaaggagaaa tggaaatact ataattaaca taatatttgg
     1441 gaggaatgca catggccaag aaaaagattc aaaggggaag taggaacaaa cttattccac
     1501 agcaacagag atctaaagct gacctgattg acgacagcag tcccgaaaac tatgaattgt
     1561 cagaggaatt tgcccgggaa aaccccatga agacggcccc aaacaaagag aagaaaaaga
     1621 aagataagtg aggaatcggc aggttatcag taatgtttga ctttattagg gagactaggg
     1681 cggtaaaaga aggtttgtac aatatcaagc ttgaaattcc tgaagacata tacaaaagca
     1741 tctatgaaga tttaaacgaa gacaaggcaa aagatatcct agttgaatat ctaaaatacc
     1801 gccaggatga tgccaggatc agcgacatca aaatagagca aaacaaaaaa gctcatatgg
     1861 taaacattta tgcaaatctt cactaccttg gcaacgaaaa aagcgagcaa gaagcttata
     1921 cggagaataa tatcaacttt gtgaaatgac acaaagtatg aaaaatgata taatagtgct
     1981 cgtgacaaca ataattatta cttagggggt atttcatggc acttcaaact ttcaatgcca
     2041 aggtagaatg gaaaggcggt gttgtagccg aggctactgc aagaggattt aaggtaacca
     2101 tggatgagcc ggaagaactt ggcggcacca atactgccat gaatccggtg gaaatgcttc
     2161 tatgcgcctt aggcggctgc atgagtatag ttgcaagcgt attcgcacca aaattccgag
     2221 tagaattaaa aggattgtca attgaaatag aaggcgattt ggacccggat ggatttaccg
     2281 gcaaaaaccc cgatgtgaga aaaggatttt cagacatccg ctacaagatg catattgtat
     2341 ctgattcacc cgaagcaaac atagaaaaat tgcacaagtt tattactacc cactgtccgg
     2401 taaaggacac cttagaaggt gttcaagtaa gcggatccta tgtaatagaa aaataatagc
     2461 cataacttaa accaaggcct ttgtcaaata gtcaatcgtt taaaataaat taagtataat
     2521 tggatgcttg gtcacttgct ttaaaggagc caagcatcct ttttatgtat ccaatataat
     2581 attgtccatc cgcttgcatt cctcaatata atcaaagagt cggtgatgca tttgaagcaa
     2641 ggaatgcaat ggaagcaatc caagagcctg tgatgataca ctttaaagat ctcaattata
     2701 aaggaagcag ggagtcaata gctccctgct tcttttaatc tgttcgacaa agtgattgtc
     2761 ggcaagtcac atacttaatt ggccctcggg atttcaacga tttcatcaat tttaatattg
     2821 ccgtatccat ctttttcatt taccgggaca aacttaaaga ccctgctgtt gccccaaatg
     2881 tctctgatta ctgcaacaac ccaatctccc cgctgcgagt caaaaatatc tattgcagtt
     2941 acgttgccgt aagggatgat tgattgtaat ttattggaat caatcttttc tttgtaggct
     3001 ttggacagat agttcattac ttcatcatat tttccatgca ttctggcatt gaggagcgag
     3061 tttaccgtaa gaatcctttc gtccttactt ataataggtc tatctttgat gggtttttcg
     3121 taatcgaaag aaccttcttt gatgtaagtc tttgcatatc cggttctcaa gaacttgcca
     3181 ttgctccagg tataagtata ggtttcgtaa aagtatctgc cgtcatattt cacattacta
     3241 attgaaaatc cgttggagtt gaccctgata gcaacaccct tgcctgaaaa catctgaacc
     3301 atcctcttac cctggatttt gtaaactgtt aaatatttgt aaccatgtgt gccattgtaa
     3361 atcactgcaa tttcgtatac accgtcattg tcaaaatccc tgtggatttt ctgggcactg
     3421 taaaagggcc tgtatgtgta aaacaaatca acattgatgc ttaaaggaat tacatccttt
     3481 tcaaaataaa cataagtact attatcctcg gctagtgcga tgccagaaaa gatttggaca
     3541 ataagcattg cagcaatcaa tgcaacaata tttttcttaa acatttaaac atcctcctcg
     3601 agtacaaaat tgataattaa cataatgcct gcattttgac acatataaat gctagcacaa
     3661 agggaagctt gggtcgatgt acttttgaag gaaaagctgt aaaaaggtag aaagatataa
     3721 ataatgtgga agatgaataa ttcatgcaga aattattccg cagtccacca tataaaatat
     3781 ttaatgaggt gccattgtaa atgtcagagg gaattttatt attcatccat aagtctgaag
     3841 ccaatcagct cttgatgtta ttgcttaaaa ataaaggtgc atatctttca ggtgaagata
     3901 tcagtaaaag atttggcata acccgttcgg cggtttggaa acaggtaaat attttaagag
     3961 atatgggata caaaataaaa tcatctccca ggctaggata tcatctggaa gagtctcccg
     4021 atttgctttt gccggaagaa atatgggcaa tgtcggagtt ggaggttttg ggcagtagaa
     4081 tctactatcg ttcagttacg gagtccacca acaatgatgc caagagattg gcccaggaag
     4141 gagtgcctga tggcactctg atagtagctg aaaggcagca gggtggcaga ggccggatgg
     4201 ggcgcatgtg ggattctccg gagggaggca tttggctttc tgtgatactt agaccggcgc
     4261 ttgctccggc tgaagcacca aaacttacta tcatgtctgc cgtggctgtt gccgaagcta
     4321 ttattcagtt aacgggcatt tctgcaaaca tcaaatggcc taatgatata ctcattgacg
     4381 gtaaaaaggt ctgcggcatt ctcaccgaaa tgagtgcgga aatggatttg ataaatcacg
     4441 taataattgg tataggaatc aacgtcaaca acgacaattt cccagatgaa ttgaaagaca
     4501 agagtatatc tctgaagcaa gtcaaggggg ataaaataaa ccgtgttaaa ctattggcga
     4561 gtttacttga aaagcttgaa gcttattatg taaaagctga aagggaagga tttgatgaga
     4621 tttttgatag atggagggct ctgtgtgtaa atttgggcaa aagagtgaga ataattggga
     4681 agaatgaaag ttttgaagga acggccatgg atattgatag ctcaggtgct ttgcttatta
     4741 aaactttaaa cggagggatt gtaaaagttt taagcggcga tgtatcaatt agatgataag
     4801 caaatacatt tataaataaa gaataaaaca cctccgctgt gcgaaaaata aacttgactt
     4861 tagaagaatt tgtaatttca agcgcaacgg gatctccggt attgcttgat gccaagggag
     4921 gtgttttttc tggttgataa agaacaacaa atacaagaaa tactgcaatt tgtttcgaga
     4981 cacaaaggtt cccatgcaag tcacaacata t
//
LOCUS       DAONUO010000140         4991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12220_length_4991_cov_3.755065, whole genome shotgun sequence.
ACCESSION   DAONUO010000140 DAONUO010000000
VERSION     DAONUO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12220_length_4991_cov_3.755065"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..2279)
                     /gene="metG"
                     /locus_tag="PK768_06125"
     CDS             complement(333..2279)
                     /gene="metG"
                     /locus_tag="PK768_06125"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQA60583.1"
                     /translation="MSKKAFYVTTPIYYVNAEPHIGHAYTTVIADFLARWHRLAGYDS
                     YFLTGTDEHGEKIAQAAKAAGEEPQAFVDRVSTRFKEAWKRLCIEYDDFIRTTEPRHK
                     KIVQEILQKVYDAGDIYYGEYEGYYCVGCERFLTDKELVDGCCPDHGTPPEKRKEGNY
                     FFRMEKYREWLIDYINSHPDFIRPEGYRNEVLSILSEPIGDLSISRPRERVPWGIPIP
                     WDEKHVTYVWFDALINYVSAVGYPDGEKYQRYWDSVWHLIGKDIIKPHAIFWPTMLKA
                     AGMPIYKHLNVGGFLMGPDGRKMSKTLGNVVDPFDLSEKYGADVVRYYLLSDVPYGQD
                     ASVGETHLVARYNAELANDLGNLLSRTITMIEKFCGGEIPAPKAKGSPDDELIALAES
                     LPDKLETMVNRMQLNAAIREIWRFIGRANKYIDETMPWSLAKDSSQKERLDTVLYNLA
                     EALRVAAVHISPIMPNTPAKIFEQLGIDDASLSSWESVKKWGGLPAGLKVSRKEIIFP
                     RIDEKAKEKAKAEQETKAKAKEEKQAEAKEEAANIISIEDFAKVELRIAEVLEAEKVK
                     GADKLLKLQVKIGDERRQIVAGIAKYYSPEELVGKKIVVVANLKPAKLRGELSQGMLL
                     AASNEEGLSLITVDRDIESGARVK"
     misc_binding    complement(2328..2574)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2748..3164
                     /locus_tag="PK768_06130"
     CDS             2748..3164
                     /locus_tag="PK768_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQA60584.1"
                     /translation="MKSYRKELWFETKNRREFINITPQVEECLRESQIKEGLLLCNAM
                     HITASVFINDDESGLHHDFEVFLDKLAPEKPYSQYRHNTYEDNADAHLKRTIMGREVV
                     VAVTEGKLDLGPWEQIFYGEFDGKRPKRVLVKIIGE"
     gene            3349..4404
                     /locus_tag="PK768_06135"
     CDS             3349..4404
                     /locus_tag="PK768_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60585.1"
                     /translation="MKKRILLFILILALMSTHVISFASDSVFTDIKPTDWFYNDVVQA
                     QKEGLVQGIGAGKYAPNKTISYAEYITVLVRVLGLDVTDELKGNHWASQYMHAAVKAG
                     ILTQEELIDLNYDKGITRQDMIKYTCKALGVKPITTTETIFNDLKDKNDEEVGLINTA
                     FEEYLTEGVGRTPEGLRIFGYDRQSNRAELATMALRIKAYKEDPVEYKKERAAAREAA
                     DREWEQKYGAQIKWEQLKKTGITSEDQITPEIVDTLREKAVGGYKRDDWKILDDKDFE
                     GYKKAIIDDLKNVEKLGSVPVVISKYLFYETKDEFKIKTFFVIVNGRESWIVSVGKSG
                     NKYKVRDAGVLIKDIFS"
     gene            4419..4793
                     /locus_tag="PK768_06140"
     CDS             4419..4793
                     /locus_tag="PK768_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60586.1"
                     /translation="MAKKRIVAIILVCMMCFINCYTHAYNDPNILYMNDGIRIDYRRP
                     GELEDGRYRADIERSGGEDDKEHLDSVTVFDQSKYAPKIEGGLYYRTVGYHFALLDGD
                     LIPVQEDGIILESETGKTVSEL"
BASE COUNT         1419 a         1089 c         1136 g         1347 t
ORIGIN      
        1 ctctggcaat ggcttcattg cctgtcatta tcttcttcaa ttgttctcct ctcctcgtgt
       61 tccagtcata taaaataatt ataacattct tttaaagctt ttttaacctt agggccaaaa
      121 cataattgta taaatgctcc cttgtccgtc tgtggataac ttgtgttttt atcaacaaat
      181 ttcccacaag ttatccacat attcacaagt tatccccaca aaaatgtgga taaatgcaga
      241 tcctgtgcat aattcaaagg cggaagcctg aaaattttca gttcccgcct tgttaaaata
      301 attcataaaa gatattcaaa gcattgattt gcttacttta cccttgctcc cgactcaata
      361 tccctgtcaa cggtaataag ggacaggcct tcctcgttgg atgcggcaag gagcatgccc
      421 tgtgacagtt cgcccctgag tttggcaggc tttaagttgg cgaccaccac tatctttttg
      481 cctacaagtt cctccgggct atagtatttg gcaatccccg ccacgatttg gcgcctttca
      541 tccccgattt ttacctggag ctttaaaagc ttatcggcac ccttgacctt ttccgcttcc
      601 aagacctcgg ctatgcgaag ctccaccttg gcaaagtctt caatgctgat gatattggcc
      661 gcctcttctt tggcctctgc ctgcttctcc tcttttgcct ttgcctttgt ttcctgctcg
      721 gcttttgctt tctccttggc cttctcatcg atcctgggga agataatttc cttcctggac
      781 actttcagtc ctgcagggag accgccccat tttttcacgc tttcccaaga agaaaggctt
      841 gcatcgtcga tgcccagctg ctcgaaaatt ttggccggtg tgttgggcat aattggtgaa
      901 atgtgaactg ccgcaacccg cagggcttca gccaggttgt acagcaccgt atccaggcgc
      961 tccttttgac ttgaatcttt tgccaggctc cagggcatgg tttcatcgat gtacttgttg
     1021 gccctgccga taaacctcca tatctcccta atcgcagcat ttagctgcat gcggtttacc
     1081 atggtctcca gcttgtcagg caggctttca gccagggcta tcagttcatc atcgggactt
     1141 cccttggctt tcggtgcggg aatttctccg ccgcaaaact tttcgatcat ggtgatggtc
     1201 ctggaaagga ggttgcccag gtcgttggcc aactctgcat tatacctggc cacaaggtgc
     1261 gtttcgccca cggaagcgtc ctggccgtag ggcacgtcgc ttaagaggta gtagcgcacc
     1321 acatccgcac cgtatttttc agataggtca aaggggtcca ccacgttccc tagggtcttt
     1381 gacatcttcc ggccgtccgg tcccatgagg aagccgccaa cgttgaggtg cttgtaaatt
     1441 ggcatgcccg cagctttcag catggtcggc cagaatatgg cgtggggctt tatgatgtcc
     1501 ttgccgatga ggtgccagac gctgtcccag tagcgctgat atttttcccc atcgggatag
     1561 cctacggccg aaacgtagtt tataagggcg tcgaaccaga cataggtcac gtgcttttcg
     1621 tcccagggaa tggggattcc ccaaggcacc cgttcccgag gcctcgatat ggaaaggtcc
     1681 cctatcggtt cagacagtat tgaaaggacc tcgttccggt agccttcggg ccgtatgaaa
     1741 tcgggatgcg aattgatgta atctataagc cactcccggt acttttccat gcggaaaaag
     1801 taattgccct ctttacgctt ctccggcggc gtcccgtggt cggggcagca gccgtctacc
     1861 agctccttgt cggtgaggaa gcgctcgcag cctacgcagt aatagccctc gtattcgcca
     1921 tagtagatat cccccgcatc gtataccttt tgcagtatct cctggactat cttcttgtgc
     1981 cttggttccg tggtgcggat aaagtcgtcg tactcgatgc aaagcctctt ccaggcttcc
     2041 ttaaagcggg tgctgaccct gtctacaaag gcctggggct cttcccctgc tgctttggca
     2101 gcctgtgcaa ttttctcccc gtgctcatcg gtgcccgtta aaaagtagga atcgtacccg
     2161 gcaagacggt gccagcgggc cagaaaatcc gcaatgacgg tggtataggc atggccgatg
     2221 tggggctcgg catttacgta ataaataggt gttgtaacgt aaaaagcttt cttgctcaat
     2281 ttttatcctc ctttaaagtt tatcgaatta aaaaaccccg ccccaaagca aggggcgagg
     2341 gtctctcgcg gtaccaccca aattcgccta aaaaggcctc agccaggtgc gggaaatctt
     2401 cattgaaaat ttccgatacc tgaagaattt taacggtcct tagccgtaac tgcctacata
     2461 tcgacagcta gactccgagg ccatcttcag caaagtattc ggcaccggct ttcacctgac
     2521 ccggctctct tttagcctag cccttttgcc tactctcccc ttcatagtct ttgcctagtg
     2581 attttttgaa tttataaaat atataccata tttaccgtgg gaagtcaagc tgcagggcta
     2641 aggcactctt tctaaccttg ctatcatctc atactgctga tgtttagaag atttttactg
     2701 attgcgcctt gtgttataat taagacaaaa gtccggaggt ggccattgtg aaaagctatc
     2761 ggaaggaact ttggtttgaa accaagaatc gaagggaatt tataaatatc acccctcaag
     2821 tggaagagtg cctacgggaa agccaaataa aagagggcct gctcttgtgc aatgccatgc
     2881 acataaccgc tagtgtattc ataaatgacg atgagagcgg ccttcatcac gactttgaag
     2941 ttttcctgga taaactggcc cccgaaaagc cctacagcca atatcggcac aacacctacg
     3001 aggacaatgc cgatgcccat cttaagcgta ctataatggg cagggaggta gtcgttgcgg
     3061 taaccgaggg caagctggac ctggggccat gggagcagat attttacggc gagtttgacg
     3121 gcaaaaggcc aaaacgggtc ctggtaaaaa ttatagggga atagaaaact tagctaacgg
     3181 ctcaatagta ttagttttat atcaaaccca ttcttctagc agaattgcca ctctttttaa
     3241 taccattatc cttcattaga atacagaata atgcaggaaa tactgataag tcgtagaata
     3301 actaaaaaag atatgtttct tgtaactatg aatcttggag gaaataatat gaaaaaaaga
     3361 attttactat ttatcctcat tctagcattg atgtcaacac acgttatttc gttcgcttct
     3421 gattctgtgt ttacagacat caagccgacg gattggtttt ataacgatgt tgtacaagca
     3481 caaaaagaag gtttagtcca gggaataggc gctggcaaat acgcgccaaa caaaacaata
     3541 agctatgcag aatacattac agttttagta agagtattgg gattggatgt aacagacgaa
     3601 ctcaaaggga atcactgggc cagccagtat atgcacgcag ccgttaaagc gggaatctta
     3661 acgcaagaag agctgataga tttaaactac gacaagggaa taacaaggca ggacatgata
     3721 aaatacacct gcaaagcgct aggtgtaaag cccataacaa ccacagaaac tatttttaat
     3781 gatctaaaag acaaaaatga tgaagaggta ggcctcatca atactgcgtt tgaagaatac
     3841 ttaaccgaag gggtaggcag aacacctgaa ggattaagaa tctttggata tgataggcaa
     3901 tcaaatagag cggaacttgc aacaatggca ttgaggataa aagcgtataa agaggaccct
     3961 gtagaatata aaaaggaaag agcggcagct agagaggcgg cggataggga atgggaacaa
     4021 aagtatggag cgcaaattaa atgggagcaa ttgaagaaaa ctggaataac gtcagaagac
     4081 caaataacac ctgaaattgt agatactttg agagaaaagg cggttggggg ttataaaagg
     4141 gatgattgga aaatattgga tgataaagac tttgagggat ataagaaagc tattatagat
     4201 gatttgaaaa atgtagaaaa attgggaagc gtaccagtag taattagtaa atacttattt
     4261 tatgaaacaa aagacgaatt taagataaag actttttttg ttatcgttaa cggaagggaa
     4321 tcttggatag ttagtgttgg aaaatctggt aacaaatata aagtgcgtga tgcgggagta
     4381 ttaataaagg atatattttc gtaggggtgg gaaatttaat ggctaaaaag aggatagtag
     4441 ctataatctt agtttgtatg atgtgtttta ttaattgtta tacgcatgca tataatgacc
     4501 ctaatatatt atatatgaat gacggcatac gaatagatta tagacggcct ggagaattgg
     4561 aggatggaag atatagagca gacatagaac gcagcggagg agaagacgac aaagaacatc
     4621 tagatagcgt tacagtattc gaccaatcca aatacgctcc aaaaatagaa ggcggtttat
     4681 actatagaac agtcggatac cactttgcac tattagacgg tgacctcata ccagttcaag
     4741 aggatggtat aatcctagag agtgagactg ggaagactgt tagtgaactt tgaagataac
     4801 caagtaaaca taggactagg atgtaattaa tatttttaga tacttaattg accctttgcc
     4861 agatttgcta gtgagaaaga ttcttcacaa tgtttagaga taaagacttg ccgacattag
     4921 cgtcatataa agaaccatca aatcatccat attacatctt agactaaaat ataaaaaaac
     4981 gccccaagat g
//
LOCUS       DAONUO010000141         4949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12344_length_4949_cov_3.774826, whole genome shotgun sequence.
ACCESSION   DAONUO010000141 DAONUO010000000
VERSION     DAONUO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12344_length_4949_cov_3.774826"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..498)
                     /locus_tag="PK768_06145"
     CDS             complement(37..498)
                     /locus_tag="PK768_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60587.1"
                     /translation="MKNQVESVQEGPEKSLRSSLDNEELLAKVVEFFPYPIQIFSLDG
                     TARYINKAALDMIGIRSVESHVGKYNVFEDPVVRKLGFMNQVRQVLTGKTVNITDFNA
                     PYRDLIRYFNVVDRDVQMISSDITCFPLVDEDGTFEYFAVVFILKKIPELQ"
     gene            complement(654..1460)
                     /gene="dapF"
                     /locus_tag="PK768_06150"
     CDS             complement(654..1460)
                     /gene="dapF"
                     /locus_tag="PK768_06150"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQA60588.1"
                     /translation="MEFWKLHGTGNDFIIIDNRGNNFKDLSALAKSICHRRFGVGSDG
                     LMAAEGSKAADIRMVYFNSDGSEAAMCGNGLRCFAKFVCDAGLVNGSSFSVETGDGIK
                     KVTIVERGEAKSIIRLEMGKPENIKEMKLENYELVFMHLGVPHSVIFVDNTLGIDELI
                     DLTEKVGPVIEKNAAFPQGTNVNFVQLAGRNFLRVSTWERGAGRTWACGTGACASAVA
                     AIYCKIVDPDGEVIVEMPGGKVNVSVFDDQEFFLEGPAQLICKGYSYVSV"
     gene            complement(1594..2817)
                     /locus_tag="PK768_06155"
     CDS             complement(1594..2817)
                     /locus_tag="PK768_06155"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA60589.1"
                     /translation="MYIKRHAEDAVSKLSKMFGAVLVTGPRQVGKTTMLKKVAEHAAY
                     ITLDDPLLLATAVEQSGTFFKDNPPPVFIDEIQYAPNLFSHIKIMIDRDKKKGQFFMS
                     GSQQFHMMKNVSESLAGRLGLLTLLGLSMREKQGVSISDPFLPTDDYFTSRRQNIKNL
                     SYDDVWYNIHRGSMPELYVNKDFDWQMFYGAYVKTYIERDVRELTQVGDEVKFLRFMT
                     VAASQIGQLLNLASLARDVGISQSTAERWMSILVTSNIVYLLKPYSNNITKRMVKTPK
                     LYFLDTGLAAYLTKWITPDVLKNGAMAGAFFENFVIAEIIKSYYNKGILEPPLYFYRD
                     KNMNEIDLLIEYNGILYPLEMKKHADPNKRDIAAFSQLDKIPSVKRGPGGVICMYDNL
                     ITLKDNDRVIPVKYL"
     gene            complement(2925..3632)
                     /locus_tag="PK768_06160"
     CDS             complement(2925..3632)
                     /locus_tag="PK768_06160"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HQA60590.1"
                     /translation="MDIFETLYRHRNEEQAVPMANYMKNLFPFLGLKKPERESLTKEF
                     LKAKKKEDGIDWDFIFKCFDMPEREFQYVAINYMDKVKDKFTPDDMETIEKLITTKSW
                     WDSVDAIYIVVGHIAMKYPELKEGILLKWMKSDNIWLKRISIIFQLKYKEKTDTEFLS
                     RAILHNSKTDEFFINKAIGWALREYSKTNKEWVREFIESNELSKLSVREGSKYIYKRG
                     TISAVGSKPNKYFKSPS"
     gene            complement(3688..4686)
                     /locus_tag="PK768_06165"
     CDS             complement(3688..4686)
                     /locus_tag="PK768_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HQA60591.1"
                     /translation="MDRNKTFCEECRRDVEYIVETVSLTGKLKGEEYSYVGKKAVCNQ
                     CGSEVYVADIEDENLKALYDAYRQENGIISLEKILEIPQKYNIGKRPLSLLLGWGEMT
                     FSRYCEGYMPTKQYSDILQKIYDDPAYYNELLEKNKDNLKSLQAYEKSKRKVQGLLGK
                     ENDADSKLDLIIEYLLYKCEDITPLALQKALYYAQGFYYAFERSFLFDDDCKAWVHGP
                     VYRDIYNRYSSYQFDPIESVETFDESVFTTTEKAILDSVIKNFCCYSGKTLESFTHLE
                     EPWRRTRGDLPVDAHSNRIIPKELIGEYFVAVKEKYCMLTPGDIEEYSKDLFEQIN"
BASE COUNT         1408 a         1126 c          852 g         1563 t
ORIGIN      
        1 agagatttgt caaggtacgg agattagttc aacttgttat tgcaattcag ggattttttt
       61 gagaatgaaa accacggcaa aatattcgaa ggttccatcc tcatcaacta aaggaaaaca
      121 tgtaatgtcg gagctgatca tctgaacatc tcggtcaaca acattaaaat accggatcag
      181 gtcccgataa ggagcattaa aatcagtaat attcaccgtt ttccccgtca aaacctgcct
      241 gacctggttc ataaacccga gtttccgcac gacgggatcc tcaaaaacat tgtatttgcc
      301 cacatgggac tcaacgcttc ttatgcctat catatccagc gccgctttgt taatataacg
      361 cgccgttccg tcaagggaaa agatctgtat cggataggga aagaactcaa ccactttcgc
      421 cagcaattcc tcattatcga gagacgaccg gagtgatttc tctggtccct cttgaacgct
      481 ttcaacctga tttttcattg atacccctct tttcttctaa accgactctt ctaccccaac
      541 ttaatcaagg ctttctaagg agcttttgtc agtgacatca agatctgcga agagctgtta
      601 taggcatgaa atcatacaag ctcaaaacga tacagagaaa ccatacagag agcctataca
      661 gacacatagc tgtaaccttt gcaaataagc tgggcggggc cttccaaaaa aaactcctga
      721 tcatcaaaaa ccgatacatt cactttcccg ccgggcattt ccactatcac ttctccgtca
      781 ggatccacga tcttgcaata gatggcagcc acggcagatg cacaggcgcc ggtaccgcat
      841 gcccaagtcc tccctgcgcc cctttcccaa gtgcttaccc ggaggaaatt tctccccgcc
      901 aattgtacga aattgacgtt ggttccctga ggaaatgcgg cgttcttttc tataaccgga
      961 ccaacctttt cagtcaaatc gatcaattca tcgatgccta gtgtattatc aacaaatatg
     1021 acgctgtgcg gtacgcccag atgcatgaaa accaattcat agttttcaag tttcatttct
     1081 ttaatgtttt ccggcttacc catttccaat ctgataatgg atttggcttc gccgcgttct
     1141 acaattgtta ccttcttgat cccatcgccg gtttcaacag aaaaagagct tccatttact
     1201 agcccggcat cgcatacaaa tttagcgaaa catctcaacc cattgccgca catggcagct
     1261 tcgcttccgt cggaattgaa atataccatg cgtatatcgg cagcttttga accttctgct
     1321 gccataaggc cgtccgatcc aacgccgaaa cgcctgtggc aaattgactt ggcaagagca
     1381 gaaaggtctt taaagttgtt cccccgatta tcgattatta taaagtcgtt tccggtgcca
     1441 tgcagtttcc aaaactccat gaaaattctc cttaaatatt tctattttaa tatggccccg
     1501 ggtgaaatat gttaatgtcg atctattttt aaatcagcaa ccaccagttc aagtttgtat
     1561 tttcccatac agccaaaaat attctttaag acattataga tattttaccg gtatcacacg
     1621 gtcattatct ttcaatgtga ttaggttgtc gtacatgcaa attacgccgc cagggccccg
     1681 tttgacggaa gggattttat caagctgtga aaatgccgca atatcccttt tattcggatc
     1741 agcatgtttt ttcatctcta gaggataaag tataccgttg tactctatca gcaaatcaat
     1801 ttcattcata tttttgtccc ggtaaaaata caaaggcggc tctaaaatac ctttgttata
     1861 atagctcttt attatttcgg caattacaaa gttctcaaag aacgcaccgg ccattgctcc
     1921 gtttttcaaa acatcaggcg taatccactt agtaagatat gccgcaagac ctgtatcaag
     1981 aaaatacagc ttcggtgtct ttaccattct ttttgtaata ttgttagaat agggctttag
     2041 caagtaaaca atgttagatg tcacgagaat ggacatccag cgttcagcgg tcgattgact
     2101 tattcctaca tcccgagcaa gagacgccag gttcaaaagt tgtcctattt gacttgcagc
     2161 taccgtcata aagcgaagga atttcacctc atcgccaacc tgtgtcagct cacgcacatc
     2221 acgctcaata taagttttga cgtaagcgcc atagaacatc tgccagtcaa aatccttatt
     2281 cacatatagc tcaggcatac taccacgatg aatgttgtac cacacatcat cataggatag
     2341 attcttgata ttttgtctac gtgaggtaaa ataatcatca gtaggcaaaa acggatccga
     2401 aatgctaaca ccttgttttt ctctcatgga taaaccaagc aatgtaagta acccaagacg
     2461 tcctgccaaa gattcgctga catttttcat catgtgaaat tgctgcgatc cggacataaa
     2521 gaactgtcct tttttcttat cgcgatcaat cataatctta atatgtgaaa ataggtttgg
     2581 cgcatattga atttcatcaa taaacacagg tgggggatta tctttaaaga aggtaccgct
     2641 ttgctctacc gccgtggcaa gcaacaacgg gtcgtcaagg gtaatatatg ctgcatgctc
     2701 cgctactttt ttaagcattg tagttttacc aacttgacgg ggaccggtca caagtactgc
     2761 gccaaacatt ttagaaagtt tactgacagc atcctcggca tgtcttttta tatacatagc
     2821 agcacgctcc ttaggctgtt ttaatattat atattattat agcctaaaaa gtgcaataaa
     2881 tacaatatct taaaatttat tttaatttat aagtatgact ctgtttacga gggggatttg
     2941 aagtacttat tcggttttga tcctacggct gatatagtgc ctcttttgta aatatatttg
     3001 ctcccttctc tcacacttaa cttcgataac tcattgcttt cgataaactc ccttacccat
     3061 tctttattgg ttttggagta ctctctgagc gcccatccga tggctttgtt tataaaaaat
     3121 tcgtctgttt tggaattatg cagaattgct ctgctcagga attccgtatc ggttttttct
     3181 ttatatttta gttgaaatat tatggagatg cgttttagcc aaatattatc cgatttcatc
     3241 cattttagca ggatgccttc ttttagctca gggtatttca tggcaatatg tcccacaaca
     3301 atgtatatgg catccacact gtcccaccag gattttgtcg ttataagctt ttcaatggtt
     3361 tccatgtcat ccggcgtgaa tttgtctttg accttatcca tataattaat ggccacatac
     3421 tgaaattccc gttccggcat atcaaaacac ttgaagatga aatcccaatc gattccatct
     3481 tcctttttct tcgcttttaa gaattccttt gtcaaagatt ccctctcggg ttttttaaga
     3541 cctaaaaacg ggaatagatt cttcatataa tttgccatag gcactgcctg ctcttcgttt
     3601 ctgtggcgat ataatgtttc aaaaatatcc atctgtgccc tcctgcaaaa ttgatcgtct
     3661 tttaacccgt tctaataata ttttgcttta gtttatttgc tcaaaaaggt ccttagaata
     3721 ctcttcaata tccccaggag tgagcatgca atatttttct ttaacagcaa cgaaatactc
     3781 tccgatcaat tctttgggta tgatacgatt agaatgtgcg tccaccggca aatcgcctct
     3841 ggttcgtcgc catggttcct ccagatgtgt gaagctttct aatgtttttc cgctataaca
     3901 gcaaaagttt ttaatgacgc tatccagtat cgctttttca gtggttgtga aaactgattc
     3961 atcgaaagtt tcaacacttt caatagggtc aaactgataa gatgaatacc gattataaat
     4021 atccctgtaa accggtccat gaacccacgc cttacaatca tcatcaaaaa gaaaacttct
     4081 ttcaaaagca taataaaatc cctgggcata atacaacgcc ttttgtaaag ctaaaggtgt
     4141 tatgtcctca catttataaa gtagatattc tataattaaa tccaacttag aatccgcatc
     4201 gttttctttg ccaagcagtc cctgcacttt tcgcttactt ttttcatacg cctggagaga
     4261 ttttagatta tctttgtttt tctcgagcaa ttcgttataa tatgccgggt catcataaat
     4321 tttctgaagg atatcagaat attgttttgt gggcatataa ccttcgcaat atctcgaaaa
     4381 agtcatttct ccccagccta ataacaacga cagggggcgt tttccaatat tatatttctg
     4441 gggaatttct aatatcttct ccagcgaaat aatgccgttt tcctgacgat aagcatcata
     4501 caaagctttc aggttttcat cctctatatc tgccacatag acttcacttc cgcactggtt
     4561 gcagacggct ttcttcccaa catatgagta ctcctcccct ttaagcttac ctgtaaggga
     4621 tacggtttcc acaatatatt ccacatctct tctacattcc tcgcagaatg ttttattcct
     4681 gtccataatg tgtcctcctt ctaactttct gattgatcat cggaaacaat aatcaatttg
     4741 tttgtttcgt ttatgaaacg atataacaac cactcgttgt ccgttttcac tctcaatcag
     4801 attaaacttg gtatataaat caactgtttc ctcaatacca tgaaagttta acagcatgat
     4861 ttggggacaa aacacatata aaacttcgtg ctcaaaacat ctatctttta aggagcgagt
     4921 aaaaatcgag ttattggctg atgaaggca
//
LOCUS       DAONUO010000142         4911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12449_length_4911_cov_3.003089, whole genome shotgun sequence.
ACCESSION   DAONUO010000142 DAONUO010000000
VERSION     DAONUO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12449_length_4911_cov_3.003089"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3136)
                     /locus_tag="PK768_06170"
     CDS             complement(<1..3136)
                     /locus_tag="PK768_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQA60592.1"
                     /translation="MFKDKLWGDEKMQKSKRFLALTLAATLLLAFGMGIYMGRNTPIL
                     TEALAEEMQLKATKEDSAGVDLDTEFVLSSESPINIDHIRNNLTVEPSINFSVKRPAG
                     KNNELLIVPEEPLEPNKIYKFTLDTISPNSLKWAFQTKGEFRVVSTLPRHQSSSVPVE
                     TGIEITFSHLNFENLSDYFSISPQTTGRFEVHKKTAVFIPDRLEPETLYTVTIKKGLP
                     LAGSSQVLEEDVTFQFETQAEPTSYSFRFFKTNSEFSTKDRPLLQFNFFSWGDKALPH
                     EIEFTVYNYKSAEDFIKAQQERETIPIWAYRSREIYIEDTSKLQQVARFLAPVMEYDY
                     STFVEFPEPLPAGYYLAEINFDDLKSQTWFQVTDLSLYSAVDKENTYIWVNNLQDGSP
                     ASGARAELYGSDEAAVTDASGLVQLATPQDTSTGIYGVVSKDGLEAVITVPTWHQWDS
                     SWEKSREFAMGYWKYLYLDRSLYKPDDTVYFWGILKPRTEDAGVIDKVTAVLSTGGWR
                     ENSDIESIEVKLDDFSFTGSMKLPNLTPGYYDLEIRVGDKTVDSAGFEVQTYSKPAYN
                     IEVKPSKKAVYIGDKVDFEIKASFFEGTPAAFLPINTYIDGPGQSTVTTDEEGNATLS
                     YRPVFSNEESSSILYRRLFLTAKLPESGEITAESLVRVLNNDIDIDVSNKVEGNTATL
                     EIQLDKLTVDKVNSGQYEPWDRDAYKDGHAANHPVKIEVFLEVWERREEGKYYDFINK
                     KVETRYYYEYKKLPYLETQVTTNSAGQAVYSFPVEDRQSYLVRVSALDFRGNPAVRER
                     QVLGPGFYREYNYKWYNLEGKTVYKAGEEVNLVMKENELQLPTRAQGYLFLMSQNGIQ
                     NAYVQDSPVFKKVFDEKLIPNFLVRGVYFDGKNYHETFDSLVSYDYEERALKIDIKTD
                     KEEYRPQETVNVEVKVTDKQGKAVKAVVNLNLVDEALYALQDQYVDILRAIYSDFLDS
                     GIKTSFYSHEIPDMPPGAAEHGGEGGSERKDFKDAVLFKTLTTDNDGKARVSFKVPDN
                     LTTWRLTCQAVTKDL"
     gene            3565..4239
                     /locus_tag="PK768_06175"
     CDS             3565..4239
                     /locus_tag="PK768_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60593.1"
                     /translation="MKPSRFTLVVILLLLTMFFMTGCLNLKKETPTPQPEDQPELQKE
                     EQKTQEEQKVEDISLKEFFPLTEGSKWLYLGEGNEFASFIREVLYVREDRAQIMENNG
                     GTISASVFETTDEEIVRIFFQGEEYNETNFLDEEPNDNLVILKKPLKVGSKWETPQGS
                     REIVEIDATVDTPAGKFEDCIKVRISMENSTSNEYFKRGVGVVKREFVSEGTTVTSTL
                     EKYEIK"
     gene            4331..4771
                     /locus_tag="PK768_06180"
     CDS             4331..4771
                     /locus_tag="PK768_06180"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HQA60594.1"
                     /translation="MNNEEKYKYWLDIAEYDLETARAMYISGRYLYVAFMCQHVEKIV
                     KGLHVLYTGEEADRTHNIARIFNKIFKDPSRILLLKDADLTTKKDHYIEFFAELLFYY
                     ISERYPTYKQKLSTSISKQKAKTVLDKTEEVFEWLKSLAQCKKY"
BASE COUNT         1383 a          987 c         1081 g         1460 t
ORIGIN      
        1 aaaggtcttt ggttactgcc tggcaggtga ggcgccatgt agtgagattg tccggcactt
       61 taaaggacac ccgggccttg ccgtcattat ccgtagtcag ggtcttgaac agcaccgcat
      121 ccttgaaatc ctttcgttca gaaccgcctt ccccgccgtg ctctgcggca ccgggtggca
      181 tatccgggat ttcatgagag taaaaagatg ttttaatacc cgaatctaaa aagtcggaat
      241 atatggctct caatatatct acatactgat cctgcagggc atagagggct tcatcaacca
      301 ggttgaggtt cactactgct tttacggctt taccctgctt gtcggtgact ttcacttcga
      361 cattgacggt ttcctggggc ctgtactctt ccttatctgt ctttatgtct attttcaatg
      421 ccctttcctc ataatcgtag ctgacgagtg agtcgaaagt ctcgtggtag tttttcccgt
      481 caaaatatac tccccggacc aaaaagttcg gtatgagttt ttcgtcaaaa actttcttaa
      541 ataccggcga gtcctggacg taggcatttt ggatgccatt ttgggacata aggaagaggt
      601 atccttgggc tctcgtaggt agctgcagtt cattttcttt catgactaga tttacttctt
      661 caccggcttt atatacagtt ttgccttcta aattgtacca tttgtagttg tactccctat
      721 agaaaccggg ccccaatacc tggcgctccc ggacggctgg gttgccccta aagtccaggg
      781 cgctgactct aacaagataa gactgcctgt cttcaacggg gaatgagtat actgcttggc
      841 ctgcactatt agtagtaacc tgtgtttcta aatagggcag ctttttatat tcataataat
      901 accgggtttc cactttttta ttgatgaaat cgtaatattt cccttcttca cgtctttccc
      961 atacttccag gaaaacttct atttttaccg ggtgatttgc tgcatgacca tccttatagg
     1021 catccctgtc ccagggttca tactggccgc tgttgacttt gtctacggtc agcttatcta
     1081 attggatttc caaggtggcc gtatttccct ctactttgtt agaaacatct atatcgatat
     1141 cgttatttaa tactctaaca aggctttccg cggtaatctc accggattcc ggcagttttg
     1201 cggtcaaaaa cagccttctg tacaggatag agctagattc ctcattggaa aagaccggcc
     1261 ggtaggacag tgtggcattg ccttcttcat cggttgttac ggtgctttgg ccggggccat
     1321 caatatacgt atttattggc aaaaaggctg cgggagtgcc ctcaaagaaa gatgctttta
     1381 tctcaaaatc aactttatct cctatgtaga cggctttttt cgagggcttg acctctatgt
     1441 tataagcggg ctttgagtaa gtctggacct caaagccggc cgaatcgacg gttttatccc
     1501 cgactcttat ttctaaatca tagtagccgg gtgttaaatt gggcagcttc atactgcctg
     1561 taaagctaaa gtcatccaac tttacctcaa tgctctcaat gtcggagttt tcccgccatc
     1621 cacctgtgga aaggactgcg gtgactttat ctataacccc ggcatcttcg gtcctgggct
     1681 ttaggatgcc ccaaaagtag accgtatcat ccggcttata cagcgacctg tcaaggtaca
     1741 ggtatttcca gtaacccatg gcaaactctc tgcttttttc ccagctgcta tcccactgat
     1801 gccaggtagg aacggttatg acggcttcaa gaccatcctt tgaaaccact ccgtaaatac
     1861 cggtggaagt atcttgtggg gtcgcgagtt ggacaagtcc tgaagcatca gtgacagctg
     1921 cttcatcact gccgtaaagc tcagctcttg ctccggaagc gggagacccg tcctgcagat
     1981 tgtttaccca aatataggta ttctctttat caacggcaga gtaaagggaa aggtccgtaa
     2041 cttgaaacca ggtctggctc tttaggtcat caaaattgat ttcggccaaa tagtatccgg
     2101 caggcaaagg ctccggaaac tctacgaagg tgctgtaatc gtactccata accggtgcca
     2161 aaaagcgggc cacctgctgc aacttggaag tgtcttcaat ataaatctcc cgggacctat
     2221 aggcccatat ggggattgtt tccctctcct gctgggcttt tatgaagtct tcggcgcttt
     2281 tgtaattata aacggtaaac tcaatttcat ggggtaaggc cttatctccc caactgaaga
     2341 aattaaattg taaaaggggt ctgtccttag tcgaaaactc gctgttggtt ttaaaaaacc
     2401 tgaagctata actcgtaggt tctgcctggg tttcaaactg aaaggtcaca tcttcttcaa
     2461 ggacctggct gcttccggcg agaggaaggc ctttcttaat agtgacggtg tacagagttt
     2521 caggttcaag tctgtcaggg ataaatacgg ctgttttttt atggacttca aatcttcccg
     2581 tggtttgtgg actgatggag aagtaatcgg ataagttttc gaagtttaag tggctgaaag
     2641 tgatttctat tcctgtttca acggggacgc tcgagctttg gtgcctggga agggtggaga
     2701 cgactctaaa ttcccctttt gtctggaagg cccatttcaa ggagttcggg cttatagtgt
     2761 ctaaggtaaa cttatagatt ttattaggtt ccaggggctc ttcaggcaca atcaggagct
     2821 cattgttttt tccggcaggc cgctttactg aaaagtttat actcggttcc acggtaaggt
     2881 tattgcggat atggtcaata tttataggag attcactgga aaggacgaac tccgtatcca
     2941 aatccacgcc ggcgctgtcc tctttggtgg ctttcaattg catttcctcc gctaaagctt
     3001 cggtcaaaat tggggtattt cgccccatgt atattcccat gccgaaggcc aacaataacg
     3061 ttgctgccag ggtcaaagcc aaaaatctct ttgatttttg cattttttca tctccccaca
     3121 atttatcttt gaacaaatat ataaactcct aaatgtccta tgttcgacag agctaggata
     3181 tgaagatttg tatcagactt gcttgtaaaa agaaactatg ttaggttcaa tttaagatta
     3241 acattgtttt ttatatatat caataaatat tttttcaaaa tttctgacat gtaggagcgt
     3301 tttttaggtc tgtgcgagtg agttttgata gcatagagtg gtgcaatcaa gcggatattg
     3361 atccgaggtt ctttacgaaa aaagtgttgc ttatcaatag aaacaggaag catgggggaa
     3421 gcgcaaggac ggtttccgtt cctatgcttc tattcttctt tttgggtaag aaaactctaa
     3481 atgcccgaat tggtatggaa tgtatagcct ttccggttag agttaaacta atttatagag
     3541 atgaaaaata taggagatgt tgttatgaaa ccatcacgtt ttacactagt tgtaattctg
     3601 cttttactta caatgttttt tatgacgggc tgtttaaatc taaaaaagga aacgccaact
     3661 ccgcaacctg aagaccaacc agaacttcaa aaagaggagc aaaagacaca agaagaacaa
     3721 aaagtggaag acatcagctt gaaggaattc ttccctctga cagaagggtc caagtggcta
     3781 tacctcggtg aaggcaatga attcgcttcc tttattcgtg aggttttgta tgtgagagaa
     3841 gatcgagcac agatcatgga gaacaatggc ggtaccataa gtgcatctgt ttttgaaacc
     3901 acagacgagg aaatagttcg catatttttt caaggggaag aatataacga gactaatttt
     3961 ctcgatgagg aacccaatga taatttagta atactgaaaa aaccgctcaa agtcggcagc
     4021 aagtgggaaa cgccgcaagg gagcagggaa atcgtagaaa tagatgcaac agttgataca
     4081 ccggcaggta aatttgaaga ttgcataaag gttcgcattt cgatggaaaa ctctacttcc
     4141 aatgaatact ttaaacgtgg ggttggagtg gtgaaaagag aatttgtttc cgaaggcacg
     4201 accgtaactt ccacattaga aaaatacgag attaaatgaa gggtctcaaa agagagaccc
     4261 tatttttgtt atatttatag tgaaaaacag aaacaatttg ggtataatgt acaaaaggat
     4321 gtgatttatt gtgaataatg aagaaaagta taaatactgg ctcgatattg cagaatacga
     4381 tttggaaaca gctcgagcaa tgtatatttc tggtagatat ttatatgtag catttatgtg
     4441 tcaacacgtg gaaaaaatag taaagggcct tcacgttcta tatacgggag aagaagctga
     4501 tagaacacat aatattgcga ggatattcaa caaaatattt aaggatccaa gtaggattct
     4561 gttgttgaaa gatgccgatc ttaccacaaa aaaagatcat tatattgagt tttttgccga
     4621 gctattgttt tattatattt cagaaaggta ccctacatat aagcaaaagt tatcgacatc
     4681 tatttcaaaa caaaaggcaa aaactgtcct agataaaaca gaggaggtat tcgaatggtt
     4741 aaaatccctc gcacagtgca aaaagtatta gaaaactacg tattaaaaat agataagcaa
     4801 atacctgtta agaaagcaat attatttggt tcatatgcaa aaggaactta taataaagat
     4861 agtgatatag atatagcgat attttcagat tattttgcag gtatggagca g
//
LOCUS       DAONUO010000143         4857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12609_length_4857_cov_3.369013, whole genome shotgun sequence.
ACCESSION   DAONUO010000143 DAONUO010000000
VERSION     DAONUO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12609_length_4857_cov_3.369013"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PK768_06185"
     CDS             complement(<1..611)
                     /locus_tag="PK768_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQA60595.1"
                     /translation="MFFEQLLNGLTLGSTYSLIALGYTMVYGILELINFAHSEIYMIG
                     AFLGLVMVTVYKLPFALALLISMAGSCLIGITIEKAAYKPLRKSTRIAPLISAIGVSI
                     FLQNAVLMLAGPQARPFKTGLNVSSLRIGSLRISSLQLLILLVSVVLMIGLHFLIQKT
                     KLGQAMRATAQDKEAAQLMGIKIDQVISFTFAIGSALGGAAGVL"
     gene            complement(693..1820)
                     /locus_tag="PK768_06190"
     CDS             complement(693..1820)
                     /locus_tag="PK768_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA60596.1"
                     /translation="MFRLSKFLTVFCVVVLTLALLAGCGGKSDTNEIKIGTIGPLTGD
                     VATYGISTKNGVEIAVDQFNENGGIDGKKVVLISEDTRGDQTEAANAASKLIERDKVV
                     AIVGGVISAETMTAGPIANDAGVVMISSSSTAAGIPEIGDYIFRNCLSDEVQAVQLAE
                     YAASELGLKRFAIMFTNNDYGLSLRNAFEAKAKEIAEVVGVETYNDGEKDFRAQLTKL
                     KGANPDALYIAGYYTEAAKIAQQAKEQGLDVQLLGADGFFSPTLIELGEDAVEDAVFT
                     AGFFPDDPSENAQNFVKAYMEKFNEEPDMFAAQAYDAAMILLNALKNTGGQGGEALQQ
                     EMIKTKDFPGITGATSFTEVGDAIKDVIILKVENGKFAKIR"
     gene            2482..2766
                     /gene="groES"
                     /locus_tag="PK768_06195"
     CDS             2482..2766
                     /gene="groES"
                     /locus_tag="PK768_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQA60597.1"
                     /translation="MNIKPLGDRIVIKVLEKEEKTKGGIVLPDTAKEKPQKGEVLAVG
                     SGEIVDGKKVPLEVKVGDKIIFSKYAGTEVKIDDEEYLILRQSDVLAIIE"
     gene            2789..4408
                     /gene="groL"
                     /locus_tag="PK768_06200"
     CDS             2789..4408
                     /gene="groL"
                     /locus_tag="PK768_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777516.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQA60598.1"
                     /translation="MAKQIKFNEEARRALLKGIDKLADTVKVTLGPKGRNVVLDKKFG
                     TPTITNDGVTIARDIELEEPFENMGAQLIKEVATKTQDVAGDGTTTATLLAQAIIHEG
                     MKNVVAGTNPMVMKKGIEKAVKAVVEELKTFSKPVETKEAIAQVASISADDEEIGELI
                     AEAMEKVGKDGVITVEESKGIGTTLEVVEGMEFDRGYISPYMVTDAEKMEAVLDEPYI
                     LITDKKLSNVQDLLPILEKIVQQGKKLLIIAEDVEGEALATLVVNKLRGTLTCVAVKA
                     PGFGDRRKAMLEDIAILTGGQVISEDLGIDIKNIDVSMLGQARQVRVDKENTTIVDGA
                     GDPENIKKRINSIKAQIEETTSDFDREKLQERLAKLAGGVAVIQVGAATETELKDKKN
                     RIEDALSATRAAVEEGIVPGGGTAFIDAIPALDKINTEGDELIGVNIVRKALEEPVKQ
                     IAENAGYEGSIIVEKVKESDKGVGFDARRGEYGDMIAKGIVDPTKVTRSTLQNAASIA
                     SMVLTTEAVVSDIPEKEKNTPGMPGGGDMMY"
BASE COUNT         1610 a          968 c         1017 g         1262 t
ORIGIN      
        1 aggactcctg cggcaccacc taatgccgaa ccgatggcaa aagtaaagga gatgacctgg
       61 tcaatcttga ttcccatcag ttgggctgcc tctttatcct gggctgttgc ccgcatggcc
      121 tggccaagct tggttttttg tattagaaaa tgcagcccta tcattagaac aaccgatact
      181 agcaaaatca aaagctgaag agaagaaatt cgcaaactgc caatccttaa agaacttaca
      241 ttcaaacccg tcttaaatgg cctggcttgc ggaccggcca acatcaagac tgcattttgc
      301 aggaaaatag aaacaccgat agcgctgata agaggcgcta tacgggtaga ctttctcaaa
      361 ggtttgtaag cagctttttc gatagtgatg cctataaggc aggaacctgc catacttatg
      421 agaagtgcca aggcaaaagg cagcttgtat acagtaacca ttacgagccc taaaaaagct
      481 cctatcatgt atatttcact atgagcaaaa ttgataagtt ctagtattcc gtacaccatg
      541 gtgtatccta gtgcaattaa agaatatgtg ctacccaatg ttagaccgtt tagtaactgt
      601 tcaaaaaaca tatttagctc cattcctttc caaaggcaaa gccaatggat agataaaaat
      661 ctatccattg actgttacag tagaatttat ttctaacgaa tttttgcgaa tttgccattc
      721 tctaccttca gtataattac gtcttttatt gcgtcaccga cttcggtaaa ggaagtggca
      781 ccggtaatac cggggaaatc cttagtcttt atcatttctt gctggagagc ctcgccgcct
      841 tggccaccgg tgtttttcag cgcattcaac agaatcatag cagcgtcata tgcctgggcg
      901 gcaaacatat caggttcttc attgaacttc tccatatatg ccttgacaaa attctgtgca
      961 ttctccgatg ggtcgtctgg gaaaaatcct gcagtgaata ctgcatcttc tacagcgtct
     1021 tcaccaagtt caattagagt tggagagaaa aagccgtctg cacctaataa ttgaacatcc
     1081 aaaccttgtt cttttgcctg ctgagctatt tttgctgctt cagtgtaata tccggctata
     1141 tataaagcgt cagggtttgc tccttttagc ttggtgagct gagccctaaa atctttttct
     1201 ccatcattgt aagtttccac tccaacaact tctgcaattt ccttggcttt tgcctcaaaa
     1261 gcgtttctta aggatagacc atagtcatta ttggtaaaca taatagcaaa tcttttaaga
     1321 cctagttcgc ttgcagcata ttcggcaagc tgaacagctt gaacttcatc agataaacaa
     1381 tttctgaaaa tgtagtcgcc tatttcaggg atacctgcag ctgttgaaga tgatgaaatc
     1441 atgacaacac cggcatcgtt tgcgattgga ccggcagtca ttgtctctgc actgataact
     1501 ccgcctacaa tggcaactac cttatccctt tcgatgagtt ttgaagcagc atttgcagct
     1561 tcagtttgat cgccacgcgt gtcttccgaa atcaaaacta ctttcttgcc gtcgattccg
     1621 ccattttcgt taaactgatc aacagcgatc tctacaccgt tcttggtgct aatgccgtaa
     1681 gttgcaacat ctccggtaag aggtccgatt gttcctattt taatttcatt agtatcggat
     1741 ttaccaccac agccagccaa caaggccaaa gtcaacacca ctacacaaaa taccgtaaga
     1801 aatttcgata gtctgaacat aaaagtacct ccttaaaatt ttgagcgtat tgtaaagtta
     1861 aatgaagtga tatttttttc agacaagaaa ctcaattcgt gaatattaca gttataaatc
     1921 cgaataattt tgataatcac ccccaagtat aaaaaacagc agttcactta tgacaatgcc
     1981 acaagtggaa cctgccgctg gagtctttta tactcacggt gtactagaaa gctatctagc
     2041 tgtaccgctt ggaccagacg ctcgaaagcc ggaaccctag gtacacttcc actggaatac
     2101 gcatttgatt tttatcctat tataatctaa ttttcacaaa atgtcactag ttgagaagag
     2161 attttataaa taattttgca taaaagatgc ataattttgt attcccggga aaaaatatga
     2221 aagtagagga ataatagagt ttaaaaaatc gagatacata cagtattgag cagaaaaaag
     2281 ggcaaaaatg tgatttattc catatttaag gagaataagg gaaattgatt ttaacatgtg
     2341 cattatatat tatattaaat gaaattagca ctcacccgac gagagtgcta acaaatatgc
     2401 caactaatac ataacttttt gtttttttgc ttagccgtta atttaaaata cttaagcgac
     2461 aatttaaaaa ggagggttac aatgaacatc aagccattag gtgaccgaat tgtcatcaaa
     2521 gttctggaaa aagaggagaa gacaaaggga ggtatagttt taccggatac cgccaaggaa
     2581 aaaccccaaa aaggtgaagt attagcagtt ggctctggcg aaattgttga cggcaaaaaa
     2641 gttcctttgg aagtcaaagt tggcgataag atcatcttct caaagtatgc aggcacggaa
     2701 gtaaagattg atgatgagga gtatttgatt ttaagacaaa gtgacgtttt agcaataata
     2761 gaatagaatt gcgaggaggg aatttttaat ggcaaaacag ataaagttta atgaagaagc
     2821 acgtagagct ttattaaaag gcatagataa attagctgac accgttaagg taactttagg
     2881 cccgaaggga cgcaatgtag tcctagataa aaaatttgga actcctacaa ttactaatga
     2941 cggtgtcacc atagcaaggg atatcgaatt ggaagagccc tttgaaaata tgggagcaca
     3001 acttatcaaa gaagtagcta caaagaccca ggacgtagcc ggagatggaa cgactacagc
     3061 tacattgctg gctcaggcca ttatccatga gggaatgaaa aatgtagtcg caggaacaaa
     3121 ccccatggtt atgaaaaaag gcatagaaaa ggctgtaaaa gctgttgtag aagagctaaa
     3181 gacctttagc aaacctgtcg agactaaaga ggcaatcgca caggtagctt ccatatctgc
     3241 agacgatgaa gaaatcggcg aactgattgc agaagcaatg gaaaaggtag gcaaagatgg
     3301 agtaataacc gtcgaagaat ccaagggcat cggcaccacc ttggaagttg ttgaaggtat
     3361 ggaatttgat aggggataca tatctcccta catggttaca gatgctgaaa aaatggaagc
     3421 ggttctggat gaaccctata tcttgataac tgacaaaaaa ctttctaatg ttcaagactt
     3481 gctccccatc ctagaaaaaa tcgtccagca gggcaaaaaa ctcctgataa ttgccgaaga
     3541 tgttgaaggt gaagcactag ccacgctggt agtcaacaaa ctgcgcggaa ccttgacctg
     3601 tgttgcagta aaggctccgg gatttggtga cagaagaaaa gccatgttgg aagacatcgc
     3661 catattaacc ggtggtcagg taatttccga agacctcggt attgatatca agaacatcga
     3721 tgtaagcatg ctgggtcagg caaggcaagt acgggtagac aaggaaaaca ccacaatcgt
     3781 ggatggcgca ggagaccctg aaaacatcaa gaagcgcatc aattccatca aggctcaaat
     3841 tgaagaaacc acttcagatt ttgataggga aaaactacaa gagcgccttg caaaattggc
     3901 cggaggcgtt gccgtaattc aggttggcgc agccaccgaa accgaactta aagataagaa
     3961 aaaccgcata gaggatgctc tgtctgctac aagggcagca gtagaagaag gcattgttcc
     4021 cggcggtgga acagcattta tcgatgccat acctgctctt gacaaaatta acactgaagg
     4081 cgacgaatta atcggcgtca atatagttag aaaagcgcta gaagaaccag taaaacaaat
     4141 tgctgaaaac gcaggatacg aaggctcaat aatcgtagaa aaggtaaagg aaagcgacaa
     4201 gggcgtaggc tttgacgcac gccgcggtga atacggcgat atgattgcca aaggtatcgt
     4261 cgatcctact aaagtaacca gatctacact ccaaaacgct gccagcattg catcaatggt
     4321 tcttactaca gaagctgtcg ttagcgatat tcctgaaaaa gagaaaaaca cacctggaat
     4381 gcccggcggc ggagatatga tgtattaata aagggaccaa taaagactcg cgaatctttc
     4441 attcgcgggt cttttttctt acattaggat aaataaaaca tgaacttaaa atcctaaatt
     4501 cgaaccagaa aatgtaggta aaaacccgtt tgtatattaa ttgacataat tgcgacaaga
     4561 gataatctaa atacagaaac tcatggatta tatagaaacc ataggcaaat cccataacat
     4621 aaaaactccc atttggaaaa acaacaggat ttgcctgtta aacccttcgg atatagctat
     4681 tttgaagctg tggaaaaagg gagcaaagtg atgtcaaacc agggagtatt tgaaacccat
     4741 tactgccttt gtgttctgga agacaaacta aaagattcag acactggcca tgatggtact
     4801 gtggatggga gcttcctacc ttgtaaaaca caataaaaat catttgattg catctct
//
LOCUS       DAONUO010000144         4852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12624_length_4852_cov_3.278924, whole genome shotgun sequence.
ACCESSION   DAONUO010000144 DAONUO010000000
VERSION     DAONUO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12624_length_4852_cov_3.278924"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK768_06205"
     CDS             <1..475
                     /locus_tag="PK768_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HQA60599.1"
                     /translation="NALRMRPDRIIVGEVRSGETLDMLQAMNTGHDGSLTTGHANSPR
                     DMLSRLETMVLMAGMDLPVRAIREQIASAIDLIIHQSRLRDGSRRITHITEVQNMEGD
                     VIVLQDLFNFIQTGVDDDGRVIGEFRATGMRPKFIDRLEVSGYKLPVEVFTNNLL"
     gene            487..1452
                     /locus_tag="PK768_06210"
     CDS             487..1452
                     /locus_tag="PK768_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQA60600.1"
                     /translation="MDLYTICILVFSSAVLTVLGIDIIRNHDEKLIVSRIKKLAGEER
                     VEKSKKAPSSGKDMLRRLLARAGDLSITRRLGKNIDKKLEEADILLRGSEFVAMVIGI
                     FIGSTLLFFTLTLKLSLSIIAGIVAAIVPFVIVNSTRQRRLASFDAQIGDALTIMSNS
                     LRYGFSFIQSMDMVSKELADPIKKEFNRTIVEINLGASTETALLNLAKRVNSDDLDLI
                     VTAVLIQRQVGGNLAEVLDNIATAVRERIRIKGEIKTLTAQGRISGVIIGLLPLFLLA
                     FMFTANPGYIMELFTTRTGLIMLTFAGLGQVLGLMIIKRIVDIQF"
     gene            1464..2390
                     /locus_tag="PK768_06215"
     CDS             1464..2390
                     /locus_tag="PK768_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQA60601.1"
                     /translation="MQMLPTLTSVFGTIFALVLFVFQSLESEKNKIKERVTNVVGPQE
                     PVPIRQRELSFSLYTRFIRPMLSSIFGILAKYIPAFNEDVLARRLNEAGNPYNLLPRE
                     VMVIKYLLSCLVALCVRLFMQSSGGSGPQTFVMIIAGLTLGWLLPDVLLNSMIRERKE
                     QVCKELPEVLDLLTVCIEAGLGFDGALKKVVEKSKGVLPDELYIVLQEISMGKPRREA
                     LRDMADRLAVDDFSNFVGSIIMAEQLGISIGNVMRLQARDVRQKRRQRVEEQAMKAPV
                     KMLVPMVFFVFPAIFIVLLGPAIIQIMRVFAK"
     gene            2403..2780
                     /locus_tag="PK768_06220"
     CDS             2403..2780
                     /locus_tag="PK768_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQA60602.1"
                     /translation="MAINLDKGTVLAKKVYVAKNFFSRLRGLIGTASLENEAGLLIIP
                     CSSVHSFFMRYPIDVVFLDHNNRVLKILYSLPPYRIGPMVKGARKALELKAGVVSATG
                     TEIGDQIVFSGEDGKNGEIFKNT"
     gene            2755..3735
                     /locus_tag="PK768_06225"
     CDS             2755..3735
                     /locus_tag="PK768_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HQA60603.1"
                     /translation="MARSLKIPRGFSLTFRLSLAFTLLIIFLMGSVIFSVFINDRNTM
                     TQNVVERGWTSVNSINAVAGDSLKSGRLDVLYEFLENMAADGFIIEALVRDLDGNLVA
                     YNGPEGQAEGILTGNTYEKAEAKKLSSIKNEKGEITAFVFSSPLSDESGATIGYMQVL
                     VDFKPTMEHLQRTGYSLIYLFLATVFIGLIVARQVVEKSVARPVKDMVKATEKVSIGD
                     FSQRLDLERNDEIGRLAQSFNIMNDQLGLLFASIKNAVTEMSASSKLIVHRTEGIEEA
                     SPTVQKEFIKEIKHSAKILNRMSLQLDSLVSQFKTQSAQEEFGTSDPAGI"
     gene            3792..>4852
                     /locus_tag="PK768_06230"
     CDS             3792..>4852
                     /locus_tag="PK768_06230"
                     /inference="COORDINATES: protein motif:HMM:NF017221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQA60604.1"
                     /translation="MVDISKLDRIIQKTKKIVEKSREELYQLGEAARRDYEHTKQELA
                     EIKVKIKDTIQEVDKLEKEFHQARIRLVEVSRNFNEYGEDEIKEAYDHAHKKQIELLE
                     KREKEKLMRLHRDYLARTLKNLEITIKRSEELITNVGMALKLLCNDLESASAQIGEVQ
                     QIQALGLSIIMAQEEERKRVAREIHDGPAQSLANIVMRAEFCLKLMEKNPALLKDELQ
                     SLMDLVRGSLQDVRKIIFDLRPMSLDDLGLVPALKRYVEQYTRDNNIFVEITVLGDEY
                     DIANSLVIAIFRVIQESMTNVRKYANATEVVIKIEYLPDRINVVVRDNGQGFDVEKVL
                     SEKNGVAFGIVGMRERIQL"
BASE COUNT         1425 a         1016 c         1211 g         1200 t
ORIGIN      
        1 gaacgccctt cgcatgaggc cagaccggat aatcgtcggc gaagtccgaa gcggggagac
       61 cctagatatg ctgcaagcca tgaatacggg ccacgacggc tcccttacaa cgggccatgc
      121 caattctccc agggacatgc tgtcgaggct tgagaccatg gtgctaatgg caggcatgga
      181 tctccccgtc agggccatcc gggaacagat tgcatcggca atagatctca taatacatca
      241 gagccgcttg agggacggtt cccgccgcat tacgcatatt accgaagtgc aaaacatgga
      301 gggtgatgtg atcgtgctcc aagatttatt taatttcata caaaccggcg tagatgatga
      361 cggcagggtc atcggtgaat ttagggcaac gggaatgagg cctaaattta tcgatagatt
      421 agaggtcagc ggctataaac tgccggtgga ggtttttaca aataatctat tataggagtc
      481 gaaagcatgg atctttacac aatatgcata ctggtttttt cttcagcggt tttaacggtt
      541 ttaggcattg atataattcg aaaccacgat gaaaagttaa tcgtcagcag aatcaagaaa
      601 ttagccggag aggaaagggt tgaaaaatcc aaaaaggcgc cgtcatcggg aaaggacatg
      661 ctaaggcgct tactcgcaag ggccggcgat ctttccataa ccaggagact tggaaaaaac
      721 atcgataaaa agctggaaga ggcggacata ttgctgcggg gcagcgaatt cgtagcaatg
      781 gtaataggta tttttatagg aagcaccctg ctgtttttta ccctcacatt gaagctgtct
      841 ttatcaatta tcgccggcat tgttgccgca atcgtacctt ttgtcatagt aaactccacc
      901 cgccaaagaa ggctggcaag tttcgatgct cagattggcg atgcccttac catcatgtca
      961 aattcccttc gatacggttt tagctttatt caaagtatgg acatggtgag taaggaactg
     1021 gcagacccta taaaaaaaga attcaacagg accattgtgg aaatcaacct tggtgcaagt
     1081 acggaaaccg cccttttaaa cttagcaaaa agggttaata gtgacgacct ggaccttatc
     1141 gtaactgcag tgctaataca aaggcaggtg ggcgggaatt tggccgaggt gttagacaac
     1201 attgctacag ccgtcaggga acgcattcga ataaaggggg aaatcaagac ccttaccgcc
     1261 cagggccgca tctccggtgt aatcataggc ctcttgcccc tcttcctgct ggctttcatg
     1321 tttactgcga acccgggata tatcatggaa ctttttacaa caagaacggg ccttataatg
     1381 ctcacatttg ctggcctagg tcaagtacta ggtctcatga tcataaagag aatcgtagat
     1441 atacagtttt agggggcatt gtcatgcaga tgttaccaac tttaacatca gttttcggta
     1501 cgatatttgc tttagtcctg tttgtctttc aatcactaga aagcgaaaaa aacaaaatca
     1561 aggagcgggt tacaaatgtg gtaggacccc aggaacccgt tcctatacgc cagcgggaac
     1621 tctccttttc cctctacacg cggtttatca ggcccatgct gtcgtcaatc ttcggcatac
     1681 ttgcaaaata catccccgcc tttaatgaag atgtgctggc caggagattg aatgaagcgg
     1741 gaaatcccta taatctgctc cccagggaag tcatggttat aaaatacctg ctgtcctgcc
     1801 tcgttgccct ttgtgtaaga ctattcatgc agagttccgg cggctccggt ccccagactt
     1861 ttgtcatgat catagccgga cttacattgg gctggctctt gcccgacgtg cttttaaact
     1921 ccatgatccg ggaaagaaaa gagcaggttt gcaaggagct gcctgaagtt ttagacctgc
     1981 tgaccgtgtg catagaggcg gggctgggct ttgatggggc cttaaaaaag gttgttgaaa
     2041 agtccaaagg ggtattaccc gatgagctat acatagtttt acaggaaatc agcatgggca
     2101 agcccaggcg tgaagcacta agagacatgg ccgaccgctt ggcggtagat gacttttcca
     2161 actttgtggg ctccattata atggcagaac aattgggcat aagcatcggc aatgtcatgc
     2221 gccttcaggc gagggatgta agacagaaac gccgccaacg ggttgaagag caggccatga
     2281 aggcccctgt taagatgctt gtgccaatgg tgttttttgt cttccccgct attttcatag
     2341 ttttactggg accggccatt attcaaatca tgcgggtttt tgcaaagtga ggggtgactt
     2401 ctttggcaat caacttggac aagggcacag tacttgcgaa aaaggtatat gtggcaaaga
     2461 actttttcag taggttgaga ggcttaatcg ggaccgccag tttagaaaat gaggcgggct
     2521 tacttataat accctgctct tctgtacaca gcttttttat gcgctatccc attgacgtgg
     2581 tctttttgga ccataacaac agggttttaa agatactcta ttcccttccc ccctatcgca
     2641 tcggtcctat ggtaaaaggc gcaaggaaag ctttagagct taaggcaggt gtagtttcgg
     2701 caacaggcac tgaaatcgga gatcaaatcg tatttagcgg agaggatggt aaaaatggcg
     2761 agatctttaa aaatacctag gggtttttct cttacattca ggttaagtct tgcttttaca
     2821 ctgcttataa tctttttaat gggttcggta atcttttcgg ttttcattaa tgataggaat
     2881 accatgacac aaaatgtcgt cgaaagaggc tggacttccg taaattccat aaatgcggtg
     2941 gcaggagatt ctttaaagtc gggtagactg gatgtcttat atgaattcct ggaaaacatg
     3001 gcggcagacg gctttatcat agaagccctt gtccgggact tggacggaaa tctcgtggcc
     3061 tataatggcc cggaaggcca ggctgagggt atccttacgg gaaatactta cgagaaagcc
     3121 gaagcaaaaa agctcagctc tatcaaaaac gaaaaaggtg aaattacggc ttttgtcttt
     3181 agctctcccc tttcagatga gtcgggggct accatcggct acatgcaagt gctggtagat
     3241 tttaaaccaa ccatggagca tcttcaaaga accgggtaca gcttgatcta cctattttta
     3301 gcaaccgttt tcatagggct cattgtagcc cgccaagtag tggaaaagtc tgttgcaagg
     3361 cctgtgaagg atatggtgaa agctaccgaa aaagtttcca taggcgactt ttcccaaagg
     3421 ctcgaccttg aaagaaacga cgagataggg cgcttagctc aatcctttaa tattatgaac
     3481 gatcaattag gcctgctctt tgcatccata aaaaatgccg tgacagaaat gagcgcttct
     3541 tccaagctca tcgtccaccg cactgagggg atcgaggagg caagtcccac ggttcaaaag
     3601 gaattcataa aggaaataaa gcacagtgcc aagatactca atcgaatgag cctacagttg
     3661 gattccctgg taagtcaatt caagacacaa tcggcacagg aagaatttgg cacatcggac
     3721 ccggcaggaa tataagcttt tgacaaagaa caataattat acacatatat acattgaagg
     3781 atggatttgg cttggtagat atttcgaagc tggacaggat cattcaaaaa acgaagaaaa
     3841 tcgtcgaaaa aagccgcgaa gagctatacc agttgggaga ggcagcccgg cgcgattacg
     3901 agcacacaaa acaagaattg gctgaaataa aagtaaaaat taaagacact atccaggaag
     3961 tggacaaatt agaaaaagaa tttcatcaag ccaggattcg cctggtagag gtcagcagaa
     4021 atttcaacga atacggcgaa gatgaaataa aagaggctta tgaccatgcc cataaaaagc
     4081 agatagagct tcttgaaaaa agggaaaaag aaaaactcat gcggctgcac agggactacc
     4141 tggcgaggac tcttaagaat cttgagatca ctataaagcg ttccgaggag ttgattacta
     4201 atgttggcat ggccttgaaa ctcctttgca atgaccttga gagcgccagt gcccaaatag
     4261 gtgaagtgca gcagatacag gccctggggc tttcaataat tatggcccag gaagaggagc
     4321 gcaagagagt cgcccgggaa atccatgacg ggcccgccca gagcctggca aatattgtca
     4381 tgcgggcgga gttctgttta aagctgatgg aaaagaaccc cgccctgtta aaagatgaac
     4441 tgcagagcct aatggattta gtccggggaa gccttcaaga tgtgcgcaaa attatttttg
     4501 atttaaggcc tatgtccctg gatgacctcg gcctggtacc tgccctgaaa agatatgtgg
     4561 agcagtacac aagagataat aatatattcg tggagataac cgtattgggg gatgaatacg
     4621 acatagccaa ttccctggtc atagcaattt ttagggtaat acaggagtcc atgacaaatg
     4681 tgagaaagta tgcaaatgcc acggaagtag tcataaaaat cgagtacctg cccgatagga
     4741 ttaacgtggt cgtcagagac aatggccagg gttttgacgt ggagaaagta ttgagtgaaa
     4801 aaaacggagt agcttttggt atagtcggta tgagggagcg tatacagcta tt
//
LOCUS       DAONUO010000145         4832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12692_length_4832_cov_3.813691, whole genome shotgun sequence.
ACCESSION   DAONUO010000145 DAONUO010000000
VERSION     DAONUO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12692_length_4832_cov_3.813691"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK768_06235"
     CDS             <1..406
                     /locus_tag="PK768_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQA60605.1"
                     /translation="DLIGLTEEHVINLLQPVLDERADMTIGIFNSGRGATDFAQKIAP
                     FLSGQRAICKSVLEKLEEYKVTSYGIEMALTLMAEKEKIRTQLVYLPNLTHLMKEEKR
                     GVLLGFLSRIKMYWDILFIFGLIKLKVIFDSK"
     gene            430..4689
                     /locus_tag="PK768_06240"
     CDS             430..4689
                     /locus_tag="PK768_06240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HQA60606.1"
                     /translation="MTKVTQIFKKKDLMLFTTEEREILFKAILLNVKVSVKSREIDIY
                     LLSEFIFSEQILKKIEKLISSHLPKTARVSIHCEILDTDNKGQRLIEKARNGLLKCLF
                     SQVPGCRVWLANSHWELNGQNLNIFIDSCGKGILKKRNLHTLIEDYFKKLGYTFKVTF
                     YEKVEENTYDVNEEEEEIIKAILEENGHNNTNSDRDSAITPNIIGKIIPGNSVPIGDN
                     LKETKEIIFSGEIFQVNKRQIKNNCTLFSFGLTDYTGSIEVKIFLSDDKKSLEHQFKE
                     GLWLKVRGKVQINNHTQEYELVPFDVNIEKKPERVDTSLKKRVELHLHTRYSSMDALC
                     PPTEVLKLAKKWGHKAVAFTDHGVLQAFPEIYEASKKLGIKPIYGLEAYIFDDENPVM
                     ISPPDVAIKDTTFVVVDIETTGLCFDADEIIEIGAVKMVNNEIVDRFNTFVKPTKPVP
                     VNITSLTGITNDMLKSAPPIEKAIVSFIEFLSDGIFVAHNAQFDSGFIRRDTSRLNIP
                     FNNKILDTLALSRILFPQLKNHRLDTLAKELKIKMGSHHRAVDDANTDALILKELLKR
                     VRDAGIDNLSRINDIYRLQKGVSNLNSFHATVLVKNDTGMRNLYKLVSIAHLNHFYRH
                     PRIPKSLLRKNREGLLIGSGCQAGELFQSLLQYGSQEKIERLLDFYDYLEIQPLHNNA
                     FLIDNENVSGMDRLKELNRKIYYLGKEANKPVIATGDVHFINPEDEIYRKILLNAQGY
                     KDADKESNLYFRTTDEMLEEFKYLGTDKAEEVVIESPNLLAEQIEEDVKPFPDELYPP
                     KIPGAEQKVIDMTYEKAKEMYGENLPEIVESRLQRELDSIINNGYAEIYLIAHKLVAK
                     SLEDGYLVGSRGSVGSSLVATMCNITEVNPLPAHYLCRKCRTSIFEHDVERIVGPDLP
                     DKNCPNCNSPMSKEGFNIPFEVFMGFEGDKVPDIDLNFSGEYQASAHKYTEELFGKNH
                     VFRAGTISTIAEKTAFGFVKTYLEERGLDVPSIEVNRLAKGITGVKRTTGQHPGGLIV
                     VPKEKEIYEFSPIQHPADDKSAGVITTHFEYHSISDRLLKLDLLGHDDPTVIKMLEEE
                     TGVNAKDIPLDDHKTMELFSSTKPLGLDPEALGTTVGTLGVPEFGTKFVRQMLEATRP
                     SSFTELVRISGLSHGTDVWLNNAHDLIKNNIATLSQVIATRDDIMIYLINKGINPKAA
                     FNIMEDVRKGKGLKPEYEKLLAENNNVDSWFIESCKKIKYLFPKAHAVAYVVMAFRIA
                     YFKVHYPEAFYATYFTVRVDDFDAELILQGPKQIKETMVEIEKKDKEASTKEKNLLTV
                     LEVANEMYMRGIQFVPIDLYKSDVQRFKITRDGILPPLSALQGLGITAAQNITKEREK
                     GSFTSIEDLKQRTKITKNVVQIMRRNGIINNLQETDQISLF"
BASE COUNT         1685 a          803 c          989 g         1355 t
ORIGIN      
        1 agatttaatc ggtttaacgg aagaacatgt aataaattta ctgcagccgg ttttagatga
       61 aagggcagat atgactattg gaatattcaa ctcaggaaga ggagctacag actttgcaca
      121 aaagatcgcc ccctttcttt ccggtcaaag ggctatttgc aaatccgttt tagaaaaact
      181 tgaagaatat aaagtaacaa gttacggaat tgagatggcc cttactttaa tggctgaaaa
      241 agaaaaaata agaacccagt tggtctactt gccaaatcta actcatttga tgaaagaaga
      301 gaaaagagga gttcttttag gattcttatc tcgcataaag atgtactggg atatactgtt
      361 tatcttcggc cttataaaac taaaagtaat atttgattca aaataactgt gtatagtgca
      421 ggggatcata tgacaaaagt cactcaaata tttaaaaaaa aggatttgat gttgtttaca
      481 acagaagaaa gagaaatact ttttaaggct attttattaa acgttaaagt ttcggtaaag
      541 tctagagaga ttgatattta cctcctttcc gagtttatct tctcagagca gattttaaaa
      601 aaaatagaga aactaatttc ttctcatttg ccaaaaaccg ctcgcgtttc aattcattgt
      661 gaaattctag atactgataa taaaggccaa aggcttatag aaaaagccag gaatggcctt
      721 ttaaaatgtc ttttttccca agtaccgggg tgtagggtgt ggctggccaa tagccattgg
      781 gagcttaatg gccaaaacct taatattttt attgactctt gcggtaaagg tattctcaaa
      841 aagcgcaatt tacatacact gatagaagac tattttaaaa aactgggtta tacatttaaa
      901 gtaacttttt atgaaaaagt tgaggaaaat acttacgatg taaatgaaga ggaagaggaa
      961 attataaaag caatacttga ggaaaatgga cacaacaata caaattcaga tagggacagt
     1021 gccataacac ctaatatcat cggaaaaata attccgggta attcggtccc cataggggac
     1081 aatttaaaag agacaaagga aattattttt tccggagaaa tttttcaggt taataagcgt
     1141 cagataaaaa acaattgcac attgttcagc tttggcttga cggactatac cggttcaatt
     1201 gaagtaaaaa tttttctttc tgatgataag aaatctctcg aacatcaatt taaagaaggt
     1261 ttatggctta aagttcgcgg gaaagtacag attaacaatc acactcaaga atatgaattg
     1321 gttccttttg atgtaaatat cgaaaaaaaa cctgaaaggg tggatacgag tcttaaaaaa
     1381 agagtagaac tacacttaca tactcgatac agttcaatgg atgctctttg ccctcctact
     1441 gaagtgttga agctggcgaa aaaatggggg cacaaagctg ttgcttttac tgaccacggt
     1501 gtactgcagg cttttccaga aatctatgaa gcatctaaaa aattaggcat aaagccgatt
     1561 tacggtcttg aagcgtacat atttgatgat gaaaacccgg ttatgatatc tccacccgat
     1621 gtagccataa aagatacaac atttgtggtt gtggacattg aaactaccgg actatgtttt
     1681 gatgccgatg agattattga aattggcgct gtaaaaatgg taaacaatga aattgtggat
     1741 aggttcaata catttgttaa accgacaaaa cctgtgcctg tgaacatcac aagtttgaca
     1801 ggtataacaa atgatatgtt aaaatcggct cctcctatag agaaagcaat cgtatcattt
     1861 attgaatttt tgtctgatgg tatatttgta gcacacaacg ctcaattcga ttcaggattt
     1921 attcgaagag atacttcaag gctaaatatt ccctttaata ataaaatatt ggatacactt
     1981 gccctatctc gcatactttt tcctcaactg aaaaatcatc gtttggatac tttggccaaa
     2041 gaactaaaga tcaaaatggg ttcacaccac agggctgtag atgatgcaaa tacagatgcc
     2101 cttattttaa aggaactttt aaaaagagta agggatgcag gcatagacaa tctatcgcga
     2161 ataaatgata tatacagatt gcaaaaagga gtttcaaact tgaattcctt ccatgccaca
     2221 gtcctcgtaa aaaatgacac gggcatgagg aatctatata aacttgtgtc aattgcacat
     2281 ttaaaccact tttacaggca tcctcgaata cctaagtcct tacttcgcaa aaaccgtgaa
     2341 ggtttgctta ttggtagcgg ttgtcaagct ggagagctgt ttcaaagctt actacaatat
     2401 ggcagtcagg agaagattga aagattgtta gacttttatg actatttaga gattcaacct
     2461 ctacacaata atgcatttct aatagacaat gaaaatgtca gtggaatgga cagattgaag
     2521 gaactaaacc gtaaaatata ttatttaggc aaagaggcga ataaacctgt aattgcgacg
     2581 ggtgatgtac attttataaa tccggaagat gaaatataca gaaagattct tttaaatgcg
     2641 caaggctata aagatgctga caaagagtcc aatttgtatt ttagaaccac agatgaaatg
     2701 ctggaagaat tcaagtactt ggggaccgat aaagcagaag aagtagttat agaatcccca
     2761 aatttgttag ctgagcaaat tgaggaagat gtaaaaccct ttcccgatga attgtatcct
     2821 cctaaaatcc caggtgcaga gcaaaaagta atagatatga cctatgagaa ggcaaaagaa
     2881 atgtacggtg aaaatttgcc ggaaattgtg gaatcgaggt tgcagcgtga attagattct
     2941 attataaaca acggctatgc ggaaatatat ctcattgctc ataagcttgt ggcaaaatcc
     3001 cttgaagacg gttatttggt tggctcgaga ggttccgtgg ggtcttcttt agtggcaact
     3061 atgtgcaata taacggaggt caacccgttg ccagcccact atttgtgccg caagtgtcgg
     3121 acttccatat ttgagcatga cgtagaaaga atcgtaggac cagatttgcc tgataagaac
     3181 tgccctaact gcaatagtcc tatgagcaaa gaaggcttta atattccctt tgaggttttt
     3241 atgggttttg agggagataa ggtgccggat attgatttaa atttttcagg agaatatcaa
     3301 gcatcagctc acaagtacac agaggagctt ttcggaaaaa accatgtgtt tagggctgga
     3361 accatttcaa caatagccga aaagacggca tttggttttg taaaaactta tctagaagag
     3421 agagggctgg atgttccaag cattgaggtt aatcgacttg caaaaggcat taccggggtt
     3481 aaaaggacaa ccgggcagca tccagggggc ctcatcgtag ttcctaaaga aaaagaaata
     3541 tatgagtttt caccgattca gcatcctgca gatgataaaa gtgcaggggt catcactaca
     3601 cattttgagt atcattctat aagtgacagg cttttaaagc tagacctgtt aggtcatgac
     3661 gacccaacag taataaaaat gctggaagaa gaaactggag ttaacgcgaa agacatccct
     3721 ctagatgatc ataagactat ggaattattt tcttccacaa aacccttggg tctggatcct
     3781 gaagcattag gtactacagt cggaacattg ggtgtgcctg agtttggcac aaaatttgtc
     3841 aggcaaatgc tcgaggctac aaggccttct tcatttacgg aattagtcag aataagcggt
     3901 ttatcccatg gaaccgatgt gtggcttaac aatgcgcatg atctaataaa aaacaatatt
     3961 gccacattgt cacaggttat tgcaactcga gacgatataa tgatatatct cataaacaaa
     4021 ggcataaacc ctaaagcagc atttaatatc atggaagatg tcagaaaagg aaagggcctc
     4081 aagccggagt atgaaaagct ccttgctgaa aataataatg tagattcctg gtttatcgag
     4141 tcttgcaaga agataaaata cctcttccca aaagcccacg cggtagcata tgtagttatg
     4201 gcatttcgaa ttgcatattt taaagtgcat tatcctgaag cattttacgc cacttatttt
     4261 actgtcaggg ttgatgattt tgatgcggaa ctaatattac agggaccgaa acaaataaaa
     4321 gaaacaatgg tagaaattga aaaaaaagat aaggaagcct ctacaaaaga aaaaaatctg
     4381 ctcactgttt tagaagtggc aaatgaaatg tacatgagag gcattcaatt tgttcctatt
     4441 gacctataca aatcagacgt ccaaaggttt aaaataacgc gagatggtat tttgccgcct
     4501 ttgtcggcgc tccaaggtct tgggattaca gcggcgcaaa acataaccaa ggaaagagaa
     4561 aaaggaagct ttacttcaat tgaggacctt aagcagcgaa ccaagattac aaaaaatgtg
     4621 gttcagatta tgcggcgaaa cggaataata aataaccttc aagaaacaga tcaaataagt
     4681 cttttttagt tgcaaacagt atgtggctgt gttataattt aaatgaaata tattttgacg
     4741 tcacgacaga tagagtgggt atattcccac tctttaatat tataaagtgt ttattgattg
     4801 caattataat cgtctatgac ttgtcaagga ct
//
LOCUS       DAONUO010000146         4826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12709_length_4826_cov_3.651855, whole genome shotgun sequence.
ACCESSION   DAONUO010000146 DAONUO010000000
VERSION     DAONUO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12709_length_4826_cov_3.651855"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK768_06245"
     CDS             <1..305
                     /locus_tag="PK768_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA60607.1"
                     /translation="GGIDMAIVSAKEAVKSILSGWTDYEKNLRAIIANKLKFEGLLVR
                     VLCERNLAEIDKLISSLKKYNLHRLLANPKLLNNFSIQLLERALMKREQLATKKVL"
     gene            complement(326..850)
                     /locus_tag="PK768_06250"
     CDS             complement(326..850)
                     /locus_tag="PK768_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HQA60608.1"
                     /translation="MKRTLIASTLVLCLIFALSSCSISKKPAPKPAPPAKMTPTPQTP
                     TPTDPTTPIPDRKPATPAHTQASKLAQAAEDVEGVKAATVVITGSTVLVGLETEPGIE
                     DEKTEDIKKRVIEAIKKADNSIKTVSITTDPNLITRLKKIAKGIKEGKPISSFTDELA
                     EITRRMTPKTKTNK"
     gene            980..1417
                     /locus_tag="PK768_06255"
     CDS             980..1417
                     /locus_tag="PK768_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQA60609.1"
                     /translation="MFELLSRTLRYVFILLIYYFLYNFLKIMIADLRREKSQPKETGF
                     VLQDEDGKNFPLFEINTIGRAEDADIIIDDPFISSKHALIYKRGSKLLIQDLHSTNGT
                     FLNDKRVTKPLRLRENDEITLGTKKFVFLRGEAVGARDGSNLQ"
     gene            1377..2609
                     /locus_tag="PK768_06260"
     CDS             1377..2609
                     /locus_tag="PK768_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQA60610.1"
                     /translation="MKQLEPGTEAIYNKNVAIAALVGILASLLLSFKEGVFSPVPLYG
                     SLIFIVIMFVGHFLSNQLFLEQDPALFILCCFISQLGLIMLYRIRPESAVKQLAWFGI
                     GFVLYFISSFASKSWIDKNIGSLIFFLATVALLASPLLFGVELYGSKSWLAFENFLFQ
                     PSELAKITFSLFLAESLKGFKIERPIVFFGQILVILSLLALARDLGGAMLFYFTALII
                     IFAATSRWDLTGFGLIGAVLAGFLGYRFFGHVRVRAKAWLNPWEDVPGKGYQIVQSLF
                     AMAEGGFFGTGLGLGHPDYIPAVTTDFIFSAFFEEFGFLGASALIVLYFLFVYRGIKI
                     SLTIDNSFLSLSALSITVFFGIQIFTIIGGVIKLIPMTGVTLPFMSYGGSSMVMSFIS
                     LGILNGIKMRASGGESDE"
     gene            2602..4014
                     /locus_tag="PK768_06265"
     CDS             2602..4014
                     /locus_tag="PK768_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA60611.1"
                     /translation="MNKLRSNIKLIFIVFTLLFISLVVYLTHFITVERERLIQSSYNR
                     RLWEQEAKVMRGTIYDSKGRPLAETGYEGGIKKRIYSGGKAFGPLIGYSDPVLGRAGL
                     ESVFNGELLGVSEQDPVTLLRQKILGVSERGSDVYLTIDLDLQRTAYDLFQGRKGALA
                     AIEPQTGRILALVSSPGYDPSNVKENWDNLSEDENKPLINRATQGLYPPGSVFKIITL
                     AAALSYNPEIETEKFYTPGYIKIDGSIIRDGQHLWPGEYDLTTAFRFSSNTVFIQIGQ
                     SVEREKMISMVEAFGFNRATKSDIPIAKSTFPKPPIIGGNVEMAESFIGQGRLLCTPI
                     QMAQVAAVIANNGNTVTPYIAREIVSPLGTVKDTLPKVSQSQVISPEVADKIKALMID
                     VVRNGTGKAAAIKGVEVAGKTGSAENPHGQAHAWFVGFAPAEDPKIAVAVVVENAGSG
                     GAVAGPIAREVILKYLSGMV"
     gene            4047..>4826
                     /locus_tag="PK768_06270"
     CDS             4047..>4826
                     /locus_tag="PK768_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQA60612.1"
                     /translation="MKKSLIADGALLFVAICWGLNFVVEKNVLSTISPIMYLAFRFLL
                     SSLLMAIVFHKRLKSINKEDIKAGLVVGTFMMLGFLTQTVGLVYTTPSKSGFITGSNV
                     VMVPFFAYLLTKRFPGSSQILGAVITFIGLGIISISDNLSIAYGDILTLLCAISFALQ
                     ITFTEHYVKKADPINMATIQVLLTGIVTMAMSLVNEPVNFDLGLENWAAILFGAVFCT
                     AGAFAVQNVAQKYTSSTHAAVILCTESVFAGIFSFLFWREPL"
BASE COUNT         1286 a          957 c         1152 g         1431 t
ORIGIN      
        1 gtggcggcat tgatatggca attgttagcg ccaaagaggc cgttaaatcc attttgtcag
       61 gatggacgga ttatgagaaa aacttgagag ccataatcgc taataaactc aaatttgaag
      121 ggctgctggt aagggtattg tgcgaaagaa atttggctga aattgataaa ttaatttcca
      181 gtttgaaaaa gtacaactta caccgcctcc tagcaaatcc aaagttgttg aacaactttt
      241 caatccaact tttagagaga gcacttatga aaagggagca gcttgcaaca aaaaaggtcc
      301 tgtgacattc acaggacttt tcattttact tatttgtctt tgtctttggt gtcatgcggc
      361 gggtgatttc agccaattca tcggtaaagc tggaaatggg tttaccttcc ttgatgcctt
      421 tggctatttt cttaaggcgt gttatgagat ttggatcggt ggtaatactt acggtcttta
      481 tactgttatc cgctttttta attgcttcaa taacccgctt tttaatgtct tctgttttct
      541 catcttctat gcccggctcg gtctcaagac ccaccagcac cgttgagccg gttataacaa
      601 ctgtagccgc tttgactccc tcaacatctt ctgcagcctg cgctagctta gatgcctgag
      661 tatgggcagg ggttgcaggt ttcctatcag gtatcggtgt tgtagggtcg gtaggcgtgg
      721 gagtttgcgg tgtaggggtc atttttgcag gaggtgcagg ctttggagct ggttttttgc
      781 taatagaaca actacttaaa gcaaatatca agcacaaaac taaggttgaa gctattagtg
      841 ttcttttcat gactttgtcc ctcctatcta attttatgat aagatttttt aaaagttttt
      901 atactgtaag attcaggcca agagcctccc atagtttacc aatggccttt tgtggtataa
      961 tatacgagag gtggtttttg tgtttgagct cctgtctcga accttaagat acgtttttat
     1021 tcttttaata tattactttt tatacaattt tttaaaaatc atgattgcag atttgcgaag
     1081 agaaaaaagc cagcctaagg agaccggctt tgttttacaa gacgaagatg gcaaaaactt
     1141 tcctctgttt gaaataaata caatcggcag ggcggaagat gcagatataa tcatcgacga
     1201 tccttttatt tccagcaagc atgctttaat ttataaaagg ggctcaaagc tattaattca
     1261 ggatttgcac agcacgaatg gcacattttt gaacgacaag agagttacca aacctttgag
     1321 actaagagaa aatgatgaaa tcaccttggg caccaaaaag tttgtatttt taaggggtga
     1381 agcagttgga gccagggacg gaagcaattt acaataagaa tgttgccatt gccgccttag
     1441 taggaatatt ggcatctttg ctcctctctt ttaaagaggg cgtgttttct cccgtacctc
     1501 tttacggaag cttaatattc atcgttatca tgtttgtagg ccacttcctg tccaatcagc
     1561 tttttctcga gcaggacccg gcacttttta ttttatgctg cttcatatct caattaggct
     1621 tgataatgct ttatagaatt aggccggagt ctgcagtcaa gcagcttgcc tggtttggga
     1681 taggatttgt gttgtatttt ataagctcct ttgcctcaaa aagctggatt gacaaaaata
     1741 taggctccct tatttttttt ctcgcaactg tagccttact tgcttcgccc ttgcttttcg
     1801 gagtggagct gtacggctca aagagctggc tggcatttga aaacttttta ttccaaccct
     1861 cggaattggc caaaattact tttagccttt ttcttgcgga gagcctgaag ggctttaaga
     1921 ttgaaaggcc aattgtattt ttcggccaga ttctcgttat attaagcttg ctggcgctcg
     1981 cacgggattt aggaggggcc atgctctttt atttcactgc tcttatcata atctttgcag
     2041 cgacttcacg ctgggacctg acaggttttg gacttatcgg tgctgttttg gccggatttt
     2101 tgggatatcg cttttttggc cacgttaggg taagggcaaa agcatggctt aacccctggg
     2161 aggacgtgcc gggtaagggt tatcaaatcg tacaatcact gtttgccatg gcagagggag
     2221 gtttttttgg tacaggtctt ggccttggtc atcccgatta tattccggcg gtgacgacgg
     2281 attttatctt ttctgccttt tttgaggaat tcggcttttt aggcgcaagt gccctaatcg
     2341 tgttatattt tttgtttgtg tatcgcggta taaagatttc gttgaccata gataatagtt
     2401 ttttaagcct ttccgctctg agcataacag tatttttcgg aatccaaatc tttaccatta
     2461 tcggtggcgt aataaagcta ataccgatga cgggagttac cctgcccttc atgagttacg
     2521 gcggcagctc catggtgatg agctttattt ctttggggat tttaaacgga ataaagatga
     2581 gagcttcagg cggtgaaagc gatgaataag ctcaggagca acatcaagtt gatatttata
     2641 gtatttacct tactcttcat ttcacttgta gtttatttaa cccatttcat caccgtagaa
     2701 cgcgagcggc tcatacaaag ctcgtataac cgcagattgt gggaacagga agcgaaggtc
     2761 atgcggggaa ccatttatga cagtaaaggg aggcctttgg cagagacggg gtatgaaggt
     2821 ggcatcaaaa agagaattta cagtggcggc aaagctttcg gccccctcat aggatattcg
     2881 gaccccgtcc tcggtagggc aggtcttgaa agcgtcttca acggtgaact actgggagta
     2941 tcggagcagg atcccgtgac gcttttaagg cagaagatac ttggagtaag tgagcgaggc
     3001 agtgatgtat acctgactat agatttggac ttgcaaagaa ccgcctatga cttatttcag
     3061 ggtcggaagg gggccttggc agcaatagaa ccgcaaacag gcagaatatt ggccttagta
     3121 agttcccccg gatacgaccc atcaaatgta aaggaaaatt gggataatct atcggaagat
     3181 gaaaacaaac ccctgataaa ccgagctacg caaggccttt atcccccggg ttcggttttt
     3241 aaaataatca cattggctgc agccctgagc tataacccgg aaattgaaac tgaaaaattt
     3301 tataccccgg gctatattaa gattgacgga agcataataa gggacggcca gcatttatgg
     3361 ccaggggaat acgatttgac cacggctttt aggttttcca gcaatacggt ttttattcaa
     3421 atagggcaga gtgtagagcg ggaaaaaatg atatccatgg ttgaagcttt tggctttaac
     3481 agggcaacaa aaagtgatat acccatagcc aagagcactt ttccaaagcc gcccattatc
     3541 gggggcaatg tggagatggc agaatccttt atcggccagg gcaggctctt atgtacgcct
     3601 atccaaatgg cccaagtggc tgctgttata gcaaataacg gcaatacagt cacgccctat
     3661 attgcccgag aaatagtctc cccattgggg accgtaaagg atacgctgcc gaaggtaagt
     3721 caatcgcagg tgataagccc tgaagtggct gacaagataa aagcattaat gatagatgtg
     3781 gtaagaaatg gtactggcaa agcagcagct attaagggag tcgaagtcgc cggcaagacc
     3841 gggagtgcgg aaaatcccca tggccaggcc cacgcttggt ttgtcggatt tgcccccgcg
     3901 gaagatccca aaatcgccgt agccgttgta gtggaaaatg caggatcagg cggtgccgtg
     3961 gcggggccaa tagcaagaga ggtaatattg aagtatttat cgggtatggt ataataaagc
     4021 agtataagac aacagaggtg aactagttga agaagagttt gattgcagat ggagcactac
     4081 tatttgtggc tatttgctgg ggattgaatt ttgtagtaga aaaaaatgta ttatcgacaa
     4141 tttcgccaat tatgtatctg gcctttagat tcttgttaag ctctttgtta atggccattg
     4201 tctttcacaa gaggctaaaa agcataaaca aagaagatat taaagcgggc cttgttgtgg
     4261 gcacattcat gatgttgggc tttttaactc agaccgtagg ccttgtttac acgaccccgt
     4321 caaagtcggg gtttattact ggcagcaatg tggtaatggt gccctttttt gcatatcttt
     4381 tgacaaagag atttccgggt agcagtcaga tacttggagc ggtaattacc tttatcggct
     4441 taggaattat ctccatcagc gacaatctaa gcattgccta tggggatata ctcacccttt
     4501 tgtgcgccat atcatttgca cttcagatta ctttcaccga acactatgta aaaaaggctg
     4561 acccaatcaa catggccaca atacaggtac ttttgacagg aatagtcacg atggccatga
     4621 gcttagtaaa tgagcccgta aacttcgact tgggtctgga aaactgggct gccatattat
     4681 tcggtgcggt attttgcact gccggtgctt ttgcggtgca gaacgttgcc caaaagtata
     4741 ccagttccac ccacgccgcc gtaatcctgt gcaccgaatc ggtctttgcc ggcatctttt
     4801 ccttcctgtt ttggcgagaa cccttg
//
LOCUS       DAONUO010000147         4751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_12958_length_4751_cov_3.390119, whole genome shotgun sequence.
ACCESSION   DAONUO010000147 DAONUO010000000
VERSION     DAONUO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12958_length_4751_cov_3.390119"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..2325
                     /locus_tag="PK768_06275"
     CDS             220..2325
                     /locus_tag="PK768_06275"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQA60613.1"
                     /translation="MVYELEGLDCAHCAAKIEHEIQKLEGLSEATVNFATRTIEIDPD
                     FEQDVAEVLQRVEPHVKLVPKKSASEKREEMAEKSKAMLRHKLLNIFLSAAFLGLGII
                     LSPRLHGSYEILEYGIFLIAYLLAGREVVETAIRNILNGQVFDENFLMTVATIGAFAI
                     HELPEAVGVMLFYSVGEYFQDLAVSRSRRSIAELMDIRPDYANLVLGNEISKVSPEIV
                     SIGQTILVRPGEKVPLDGEVLEGSSFVDTSALTGESVPRKVKTGEKVMAGTVNGSGLL
                     MVKVEKTFEESSVSKILNLVENAAGRKAPTEQFITKFARYYTPAVVFSAMAIALIPPL
                     LVPGQIFAKWFYRALTMLVISCPCALVVSIPLGYFGGIGGASRSGILVKGANFLEALT
                     NIDTVVFDKTGTLTKGVFRVTDVKALGAFAEEELLRITAHAEVHSNHPIAKSILEAFD
                     GELDEKAVRNYREIAGHGIAAEVDGKKVLAGNEKLLKREGIAVNSEDIDGTVVHVAVD
                     GSYAGYITISDELRQDAPQAIQKLKALGIKSTVMLTGDTEDTASRIARLLDLDGYHAG
                     LLPEEKVQKLEEIQVKGNQRRKVAFVGDGINDAPVITRADVVIAMGGLGSDAAIEAAD
                     VVIMEDMPSKVAEAINIARFTRKIVIQNIAFALGVKGVFLAMGAVGAASMWEAVFADV
                     GVALLTVLNSSRTLMRKTR"
     gene            complement(2559..3017)
                     /locus_tag="PK768_06280"
     CDS             complement(2559..3017)
                     /locus_tag="PK768_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HQA60614.1"
                     /translation="MAETNKIPNPEVKCLVNTCTHWLPGNLCGAGNIDILNEEVGKMS
                     QNLEQTMCKTFEERRGLANLIGSADNVNWVGFAEELVGIGRRLNPTVTCIVDTCKYYH
                     EGDLCNVDAIEITGENAKECQATNCATFEYSGRQSKNEKQQQRQEKGEKF"
     gene            3174..3857
                     /gene="deoC"
                     /locus_tag="PK768_06285"
     CDS             3174..3857
                     /gene="deoC"
                     /locus_tag="PK768_06285"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQA60615.1"
                     /translation="MLTKDELAKYIDHTLLKATATFEDIKILCLEAIQYGFYAVCVSP
                     CYVKHAAKLLRDTDVKVCTTVGFPLGAVTTATKVAEAVEAVKAGASEVDMVMNLGLLK
                     SGDYYGVQEDISEVVKAVRAHNPSGTVKVILEMALLDENEKVCACQLAKAANADFVKT
                     STGFGTGGATVEDVMLMRRLVGDIMGVKAAGGIRDARTAIEMIEAGANRIGSSSGITI
                     IQDYEHNFS"
     gene            3873..4424
                     /locus_tag="PK768_06290"
     CDS             3873..4424
                     /locus_tag="PK768_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HQA60616.1"
                     /translation="MYFEKAGKHNTEKTLEIVKQAVSERGIKRVVVASTEGYTAKLFK
                     EANLGVDLVCVTHVAGFEKPGEIEMPGEIRRELAESGVKVLTTTHVLSGAERGISNKF
                     GGIYPVELMANTLRMMGQGVKVCVEISVMALDAGLIPYGEEVIAVGGTGEGADTAIII
                     KPAHAANIFDTWISEILCKPANK"
     gene            complement(4502..>4751)
                     /locus_tag="PK768_06295"
     CDS             complement(4502..>4751)
                     /locus_tag="PK768_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQA60617.1"
                     /translation="DVVVLMVNLDVNNDRLPLYIENQGFTFTVLKDERGEAARSYLIY
                     GMPTTIFVDKDGIVSMRLEGPLNKSHLDSIVEQIRQKS"
BASE COUNT         1296 a          973 c         1197 g         1285 t
ORIGIN      
        1 gactttgggg gctcatctgg gcttcaccgc aggggttaat cccgagatga tcgtccccgt
       61 aattgcaggt aaattgtttg cagggattgt cgcactactt ttgtcttatt ttgccacaag
      121 agatttatga tcttgccttt ttcttcaacg ggtggtaaaa taataacaga gaaacatttg
      181 aacaattatt caaatataga aatgtaaagg ggtaaaatta tggtatacga attggagggt
      241 ttagattgtg cccattgcgc agccaaaatt gagcatgaaa ttcaaaagtt ggaaggccta
      301 tccgaggcta ccgtgaattt tgccacccga acaattgaaa tagatccaga tttcgagcag
      361 gatgtggccg aagtccttca aagagttgag ccccatgtta agcttgtccc taaaaaatca
      421 gcttccgaga agagagagga aatggcagaa aagagcaaag ccatgctaag acacaagctt
      481 ctcaatatat ttttatccgc cgccttttta ggtcttggaa tcatactgtc tcccagactg
      541 catgggtctt atgaaatatt ggaatatggc atatttttaa tagcgtattt gctggcaggc
      601 cgtgaggttg tagaaactgc cattagaaat atcctcaatg gccaggtctt tgatgaaaac
      661 tttctgatga ccgtggccac tatcggcgcc tttgccattc acgagctgcc tgaagccgta
      721 ggagtcatgc tcttttattc ggtaggagaa tacttccagg acctagccgt atccaggtcc
      781 cgccgctcca ttgcggagct catggacatt cggcccgatt atgcaaatct agttttggga
      841 aatgaaatca gcaaagtttc tccggaaatt gtaagcatag gccaaactat acttgtgagg
      901 ccgggagaaa aggtgcccct tgacggggaa gtgctggaag gcagttcttt tgtggacact
      961 tccgccctta ctggtgaatc ggttccgcga aaggtcaaga ccggtgaaaa agtgatggcg
     1021 ggcacggtaa atggttctgg actgctgatg gtgaaagtgg aaaagacctt tgaggaatca
     1081 tcggtctcaa agatacttaa cctggtggaa aatgcggcag gccgcaaggc gcctacggag
     1141 cagtttatca cgaaatttgc caggtactat acgccggcgg tggtttttag tgccatggca
     1201 atagctttaa tacccccact gctcgttccg ggacagattt ttgccaagtg gttttacagg
     1261 gcattaacga tgctggtcat atcttgcccc tgcgcccttg ttgtttcgat tcccctgggc
     1321 tattttgggg gaatcggagg agcttccagg agtggtatcc tggtgaaagg agccaatttc
     1381 ttagaagcgc ttacgaatat tgacaccgtt gtcttcgaca agacgggaac cctcacaaaa
     1441 ggtgttttta gggtcaccga tgtcaaagcc ctaggagctt ttgccgagga agagttgttg
     1501 cgcataacgg cgcatgctga agtgcattca aatcatccga tagccaaatc aattttggag
     1561 gcctttgatg gcgaacttga cgaaaaggcc gtgaggaact acagggagat tgcgggccat
     1621 ggcatagctg cagaggttga cggcaaaaag gtactggcag gcaatgaaaa actgcttaag
     1681 cgagaaggta tagcagtaaa cagcgaagac atcgacggca cggtagtcca tgttgcagta
     1741 gatggcagct acgcggggta tatcactatt tccgatgagc tccggcagga tgcacctcaa
     1801 gccatacaga agcttaaagc attgggtata aaaagcacag tcatgctcac cggtgatacg
     1861 gaagataccg ctagccgcat tgcaaggctt ttggaccttg atggatacca tgcggggctt
     1921 ttgccggagg aaaaagtaca aaaactcgaa gagattcagg tcaagggcaa tcagagacgg
     1981 aaagtggcat tcgttgggga cggcataaat gacgctccgg ttataacacg tgccgatgta
     2041 gtcattgcca tggggggcct tggctccgat gctgccattg aagctgctga tgtggtcatc
     2101 atggaggaca tgccttctaa agtggcagaa gcaattaata tagcaagatt caccaggaaa
     2161 attgtcattc aaaacatagc ttttgcactg ggagtcaaag gagtttttct ggccatggga
     2221 gccgtgggag ctgctagtat gtgggaagct gtatttgccg atgtgggtgt ggctctcctc
     2281 actgttctca attcaagcag gaccttaatg cgaaaaacaa ggtaaaaaag aatgcaacaa
     2341 ggagtatgtg ccttgttgca ttttacttgt gattaatttt tgatacggga tatcagcctc
     2401 cttttcctcg tatatttaaa ttagatacaa gtcgttccag gagattttaa gtatcttgaa
     2461 tcaatttcag attgttaaga tattctactc tcaagagtaa tatgccctta agtaaaaact
     2521 aatattcatc ttataacact tgtgacagca tggagctgtt aaaacttttc gcctttttcc
     2581 tgcctttgct gctgtttttc gttttttgat tgccttccgc tgtattcaaa agttgcgcag
     2641 tttgtagcct ggcattcctt ggcattttca ccggtaattt ctatggcgtc cacattgcat
     2701 aggtctccct cgtggtagta cttgcaggtg tcaacgatgc aggttacggt ggggtttaac
     2761 ctgcggccaa tgccaaccag ttcttctgcg aaaccaaccc agtttacatt gtcggcagaa
     2821 ccaatgagat ttgcaagacc ccgcctctct tcaaaggtct tgcacatggt ttgctcaagg
     2881 ttttgtgaca ttttccccac ttcttcattc aaaatgtcga tattgcccgc accgcagaga
     2941 tttcccggga gccaatgggt acaggtgttt actaggcatt tgacttcagg attcggaatt
     3001 ttatttgttt ctgccataat aacactcctc gaagcttatt ctatctatag tatttgccgt
     3061 ttttgagaaa ttattgtatt gaatgactat ccattttcct gtagattgtg tataatagag
     3121 atgtagaact tttcacaaat tttttatctt aaaattaaag gacaggtgat tttatgctta
     3181 ccaaagatga gctggcaaaa tacatagacc ataccttgct taaagcaact gccacatttg
     3241 aggacataaa aatactgtgc cttgaagcca tacaatacgg tttttacgca gtatgcgtca
     3301 gtccctgtta tgttaaacat gctgcaaaac ttctcagaga cacggatgta aaagtttgta
     3361 cgaccgttgg cttcccgctg ggtgccgtaa caactgctac gaaggtggct gaagcagttg
     3421 aagcagtcaa agccggggct tccgaagtag atatggtaat gaacctgggt ctgctgaaaa
     3481 gcggtgatta ttacggggtc caggaagata tcagcgaagt ggtaaaagcg gtaagggcgc
     3541 acaaccccag cggaacagta aaagttattc ttgagatggc cctacttgat gaaaacgaaa
     3601 aggtatgtgc atgccagctg gcgaaagctg ccaatgcaga ttttgtcaag acatctactg
     3661 gatttggaac cggcggtgca accgtggaag atgtcatgct gatgcggaga ctcgtgggag
     3721 atatcatggg ggtaaaggcc gccggcggta tcagggatgc taggaccgca attgagatga
     3781 ttgaggcagg agccaatcgc attggttcaa gttccggtat aaccatcatc caagactacg
     3841 aacacaattt cagctgagga ggttttgcat tcatgtactt tgaaaaggct gggaagcata
     3901 ataccgaaaa gactctcgaa atcgtaaaac aggctgtcag tgaacggggt atcaaacgtg
     3961 tggtagtggc atccaccgag ggttatactg ccaagttgtt taaagaagca aatctcgggg
     4021 tagaccttgt ctgtgtaact cacgtggcag gttttgaaaa gcccggagag atagaaatgc
     4081 ctggggaaat aaggcgtgag cttgcagaat ccggggtgaa ggtgcttaca acaacgcatg
     4141 tgctgtcagg ggccgaacgc ggcataagca ataaatttgg cggaatatat cctgtggagc
     4201 tcatggccaa tactctgaga atgatgggcc agggcgtaaa ggtgtgtgtt gaaatatcgg
     4261 tcatggccct cgatgcaggc ctaattccgt acggtgaaga agtaattgct gtaggcggca
     4321 ccggtgaggg tgcggatacg gcgataatca taaagccggc tcatgcagca aatatctttg
     4381 atacatggat ttccgaaatc ctctgcaagc ctgcaaataa ataattttta tattaaaaag
     4441 gcggttgcac gcaatgcagc cgcctttaac agtggaggga gcctttaact acctatgata
     4501 tttaagactt ttgccttatt tgttcgacaa tgctgtcaag atggctcttg ttgagggggc
     4561 cttcaagccg cattgatact atgccgtcct tatctacaaa aatagtagtg ggcattccgt
     4621 aaatcaagta acttcttgct gcttctcccc tctcgtcttt tagcacggtg aaagtaaatc
     4681 cttgattttc tatatatagg ggcaatctat cattatttac gtccaggttg accattaaaa
     4741 ccactacatc t
//
LOCUS       DAONUO010000148         4734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13017_length_4734_cov_3.340885, whole genome shotgun sequence.
ACCESSION   DAONUO010000148 DAONUO010000000
VERSION     DAONUO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13017_length_4734_cov_3.340885"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PK768_06300"
     CDS             <1..394
                     /locus_tag="PK768_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQA60618.1"
                     /translation="NGELNFSFSGIKTAVLNYLNHQRQKGLSIIIEDVAASFQKAVVD
                     ALVENTLKAAKVKKVKTIAVAGGVAANSRLRQELTERAASEFDVKFPPMILCTDNAAM
                     IACTGYYRYLMGDRSDMTLTPYATLKPF"
     gene            430..912
                     /locus_tag="PK768_06305"
     CDS             430..912
                     /locus_tag="PK768_06305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQA60619.1"
                     /translation="MRRVQRHQVKEVNSLWYWYKTVNPFKVVINFIVIYFARFIPHLP
                     LKNFLYRLIGMKVGKNVSVGLMAMFDIFFPELIEIGDNSIIGYNSTILAHEYMVKEWG
                     TGKVVIGRNVTIGANVTVLPGVVIGDGTTVSACSLVNKDVPENAFVGGIPIKALDIKN
                     "
     gene            complement(959..1423)
                     /gene="rimI"
                     /locus_tag="PK768_06310"
     CDS             complement(959..1423)
                     /gene="rimI"
                     /locus_tag="PK768_06310"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQA60620.1"
                     /translation="MEQRADHAFTIEMMDIKDLDEVIEVERQCFTTPWSRYSFICELK
                     DNQFSHYIVAKHMDKIVGYAGMWVIIDEAHVTNVGVLPEYRGEGVGEFLMRSLIAAAK
                     ERGANMMTLEVRKSNYVAQNLYSKLGFEPIGIRRGYYMDDREDAVIMLKEPL"
     gene            complement(1407..2126)
                     /gene="tsaB"
                     /locus_tag="PK768_06315"
     CDS             complement(1407..2126)
                     /gene="tsaB"
                     /locus_tag="PK768_06315"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQA60621.1"
                     /translation="MTNILAIDSSSIVTTVAVLNEEKLLAEYIENDKQNHSEKMMLVI
                     QKVLEDLGMTADDLDVIAVAKGPGSFTGIRIGMACAQGVAHALNKPMVGVNTLDGLAY
                     NLMHSRDLICPVINAQRQELYTSLYCFKDDKLERLWDYRLIKADKLAQELFDLNQKLI
                     VLGDGMPILQQALEKNQLSHGNIVFAHPVFSMPRASSIAAAALQEYSQGKAQDCFNIK
                     PFYIRKSNAEEKWEGRHGAKS"
     gene            complement(2123..2578)
                     /gene="tsaE"
                     /locus_tag="PK768_06320"
     CDS             complement(2123..2578)
                     /gene="tsaE"
                     /locus_tag="PK768_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQA60622.1"
                     /translation="MELVFETQNIEQTEKLGKSLGKCLSKGDFVALTGDLGAGKTAFT
                     KGVAKGLGIKDDVTSPTFTIINEYPGPVPLAHMDVYRLKSLQELENIGFDDYLDGYVV
                     VMEWADKVKELLPEDVLWIDFKVLDNSHRQIRFVSSSPYYDDKIRELSI"
     gene            complement(2703..3860)
                     /locus_tag="PK768_06325"
     CDS             complement(2703..3860)
                     /locus_tag="PK768_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQA60623.1"
                     /translation="MLAITNGKIYTMTGEVFDGGTLLIKDGKIEEVGQEVAIPENAKV
                     IDAKGKYVFPGFIDAHSHIGIFEEAVGEIGEEGNEWTDPVTPQLRAIDAINPADQAFE
                     DAVMGGITCVFTGPGSANVIGGQSVIMKTHGKIIDSMVVKAPAGLKVAFGENPKRVYG
                     EQKKMPATRMATAALLREALTNAQNYKAKREKNHDKPDEVFEKDLKMEILCEVLDKKL
                     PLRAHAHRADDIMTAVRIAREFDLDIVLEHCTEGHLIADELAKLDVPAIVGPSLSNRS
                     KVELKNLSFKTPGVLAEKGVKVAIMTDHPVIPIQYLPVCAALAVREGMKEEDALAAIS
                     IRAAEILGIADRVGSLDAGKDADVVIWNGYPLDIMSKPCLVIIDGKIAYEN"
     gene            complement(3862..4611)
                     /locus_tag="PK768_06330"
     CDS             complement(3862..4611)
                     /locus_tag="PK768_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQA60624.1"
                     /translation="MCFDKVGSIMGPLIGITMAFDHGTNTAKLSAKYISAVEEAGGVP
                     LAIPPLEDKKLLNVIIQQLDGIILTGGPDIDPSYYNQLPHRYLGRVCPRRDASEIYLA
                     AEFMKLSKPILGICRGLQVMNVAMGGTLCQDIPSIIDNPIKHMQDAPRSHKSHEIEIV
                     EQDSLTYKIIGNSRLMVNSFHHQAVGKPAPDLKVTARAPDGVIEAMESKGRGFCIGVQ
                     WHPEELTDDRAHMRLFEYLVYAAKDVEIGRA"
BASE COUNT         1194 a         1157 c         1013 g         1370 t
ORIGIN      
        1 aaacggagaa ctcaatttta gctttagcgg catcaaaacg gcagttttga attacttaaa
       61 ccatcaaagg caaaaaggct tgtcaataat aattgaggat gtggcggcca gttttcaaaa
      121 agcagtggtg gatgccctgg ttgaaaatac tctaaaagct gcaaaggtga aaaaagtaaa
      181 gacgattgcc gtagcagggg gcgtagcggc aaacagccgt cttcgccagg agttgacaga
      241 gcgggcagct tctgaatttg atgtcaaatt tccgcccatg atactctgca ccgacaatgc
      301 agccatgatt gcatgtacag gctattaccg ctatctcatg ggagacaggt ctgatatgac
      361 gcttaccccc tatgcaactt tgaagccttt ttgagcacgg gagtaaatat aatttatttg
      421 gaggggaaaa tgcgcagggt acaaaggcat caggtcaaag aggttaattc cctttggtat
      481 tggtacaaaa ccgtcaaccc ctttaaggtg gtaatcaatt ttatagtgat atattttgcc
      541 cgcttcatac cccacttgcc gctcaagaat ttcctgtaca ggctaatagg gatgaaagtg
      601 ggcaaaaatg tatccgtagg tctaatggca atgttcgaca tattttttcc cgaactgatt
      661 gaaatagggg acaattccat aataggctat aactccacga tattggccca tgagtacatg
      721 gtaaaggagt ggggtacggg aaaggttgta ataggaagga atgtgacgat cggtgcaaat
      781 gttacggttc tgccgggagt tgtaatcgga gacggcacaa ccgtatccgc ctgttccctg
      841 gtcaacaagg atgtgccgga aaatgccttc gttggcggta tacctattaa agctttagac
      901 ataaaaaatt agcctttaag aatatctaat aagttaataa gtttgtacca ggcaccgttt
      961 acaggggttc ctttaacatg attacggcat cttctctgtc gtccatgtaa tagcccctgc
     1021 gaatgcctat cggttcaaag ccgagtttgg aatacaggtt ttgcgctaca taattgctct
     1081 ttcttacttc taaggtcatc atattcgccc cccgctcttt agccgcagca ataagcgagc
     1141 gcatgagaaa ttctcccaca ccttctcccc ggtactccgg aagtacgcct acatttgtca
     1201 catgagcttc gtcaataatt acccacatgc cggcatagcc aacgatttta tccatatgtt
     1261 tggcaacgat gtagtgagaa aattggttgt ctttaagctc gcagatgaaa gaatagcggg
     1321 accagggagt agtaaaacat tggcgctcaa cttcaattac ctcatccagg tcctttatat
     1381 ccatcatctc gatggtaaat gcatggtcag ctctttgctc catgacgccc ctcccacttc
     1441 tcctcggcat ttgatttgcg aatataaaag ggttttatat taaagcagtc ctgggctttg
     1501 ccctgactgt attcctgcag tgccgctgct gctattgagg aagccctggg catggaaaaa
     1561 actggatggg caaagacaat atttccgtgt gaaagctggt ttttttctaa ggcctgctgc
     1621 agtatgggca tgccatcgcc caaaactatt agcttttgat tcaggtcgaa aagctcctgg
     1681 gccagtttgt ctgctttaat caacctgtaa tcccaaagcc gctcgagttt atcatctttg
     1741 aaacagtaca gggaagtgta tagttcctgc cgctgggcat ttatcaccgg gcaaattaaa
     1801 tccctgctgt gcataagatt atatgctaga ccatcgaggg tgttgacacc taccatgggt
     1861 ttattcaagg catgggctac gccctgagcg caagccatcc ctattcgaat tccggtaaaa
     1921 gagccgggtc ccttggcaac tgctatcaca tctaaatcat ctgccgtcat ccccaagtct
     1981 tccaatactt tctgaatgac aagcatcatt ttttccgaat ggttttgttt atcgttctct
     2041 atatattcag ccaaaagctt ttcttcattg agtacggcaa cagttgtgac aatggatgat
     2101 gagtcaatgg ccaatatatt cgtcatatgc tcaactcccg aatcttatcg tcgtaatagg
     2161 gactgctcga aacaaatcgt atttgccggt gagagttatc caacacctta aaatctatcc
     2221 agagtacatc ttcaggcagc agttccttca ctttatcagc ccactccatg acgacaacgt
     2281 acccatccaa gtaatcgtca aaacctatat tctcaagttc ctgtagactc tttagcctgt
     2341 atacatccat atgggctagg ggaactggcc ccgggtattc atttattatg gtaaaagttg
     2401 ggctggtaac atcgtccttt atgccaagcc cctttgctac gcccttcgta aaggcagtct
     2461 taccagcacc caaatccccg gttaaagcta cgaagtctcc ttttgaaagg catttgccca
     2521 aagattttcc cagtttttcc gtttgctcta tattttgcgt ttcaaatact agttccataa
     2581 tgctcatcct atttttcaaa atcattagta tttattatat cacaaatagt aaccgagtaa
     2641 atataaagca ggacaaaaag cgacacacta ccttgtaatg tgccgctaaa gttttacgaa
     2701 gtttagttct catatgctat tttgccgtct atgatcacaa ggcaaggttt tgacattata
     2761 tcaaggggat atccgttcca tatgaccaca tcggcgtcct tgcccgcatc caggctcccc
     2821 actcggtcgg ctatgcccag tatttccgcc gctcttatgc taatagcagc taaggcatct
     2881 tcttccttca tcccttcgcg gacggccagg gctgcacaaa cgggcaggta ctgtataggt
     2941 atgacgggat ggtcggtcat tatcgccact tttacccctt tttcggcgag aaccccgggc
     3001 gttttaaagc tcaaattttt caattccacc ttggaccgat tcgaaaggga tgggcctaca
     3061 attgcgggca catccagttt agccagttca tcggcaatca ggtggccttc ggtgcagtgc
     3121 tcaaggacta tgtccaggtc aaactccctg gcaatcctta cggcggtcat gatatcatcc
     3181 gccctgtggg catgagccct taagggcagc tttttatcca gcacctcaca caggatttcc
     3241 attttaaggt ccttttcaaa tacttcatcg ggcttgtcat gattcttttc ccgttttgcc
     3301 ttgtagtttt gagcatttgt cagggcttcc cttaaaaggg ctgccgttgc cattcgggtt
     3361 gcaggcattt tcttttgttc cccgtaaact cttttgggat tttcgccaaa agcaacctta
     3421 aggcctgcag gtgctttgac caccatactg tctattattt taccgtgagt tttcataatg
     3481 accgattggc cgccgatgac atttgcactg ccgggcccgg taaaaacgca tgtgattcct
     3541 cccatcaccg catcttcaaa agcttggtcg gcagggttta tggcatctat tgcccgaagc
     3601 tgaggggtta cgggatccgt ccactcgttc ccttcctctc ctatctcccc caccgcttct
     3661 tcgaatatcc cgatatggct gtgggcatcg ataaagccgg gaaaaacgta tttgcccttc
     3721 gcatcaatta ccttagcgtt ttccgggatg gcgacttcct ggccaacttc ctctattttg
     3781 ccgtccttta tcagcaaagt tcctccatca aagacttcgc cggtcatagt ataaatttta
     3841 ccatttgtta tggctaacat atcatgccct cccaatctca acatcttttg cagcatacac
     3901 caggtattca aacaatctca tatgtgccct atcatctgtc agttcttccg gatgccactg
     3961 cacgccgata caaaagcctc ttccttttga ctccattgcc tcaataaccc catcgggcgc
     4021 tctggcagtg actttcaaat cgggggcggg ctttcccacg gcctgatggt gaaagctgtt
     4081 aaccattagg cgagaattgc caatgatttt atatgtcaat gaatcctgct ccacgatttc
     4141 aatctcatga cttttgtgag atcgtggcgc atcctgcatg tgctttatgg gattatcaat
     4201 aatagaggga atatcctggc aaagtgttcc gcccatggcc acattcatca cctgcaggcc
     4261 ccggcagatt cccaaaatag gttttgatag tttcatgaat tcagcagcaa ggtatatttc
     4321 actggcatcc cttcgagggc agaccctacc aaggtatcga tggggtagct ggttataata
     4381 agagggatca atatccggcc ctcccgttaa tatgatccca tctagttgtt gaattattac
     4441 atttaaaagc tttttatctt cgaggggcgg tatggccagg ggtactccac cggcctcctc
     4501 aacggcactt atgtatttgg cgctcaattt agccgtattg gttccatggt cgaaggccat
     4561 ggtaatccct atcaatggcc ccatgatgct tcccacctta tcaaagcaca cttattgcca
     4621 gcttagctat ttccgggtca aattgggccc cggcattttg gaccaagacg atttttgcct
     4681 cgttcttgct cttggttttt tgatacggtc taaaggaagt catcgcatca aagg
//
LOCUS       DAONUO010000149         4716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13084_length_4716_cov_3.353358, whole genome shotgun sequence.
ACCESSION   DAONUO010000149 DAONUO010000000
VERSION     DAONUO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13084_length_4716_cov_3.353358"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK768_06335"
     CDS             complement(<1..424)
                     /locus_tag="PK768_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQA60625.1"
                     /translation="MWGPPRSKLNVVNIPIDEIQPNAYQPRKTFNDETLDELTNSIKN
                     YGVLQPIVVRRRGRSGYEIIAGERRWRACKRAGLKEIPAIIKDAKDMDSAILALIENI
                     QREDLNFIEEAEGYRQLIQECGVTQEQLAMKLGKSQSTI"
     gene            complement(579..1295)
                     /gene="rsmG"
                     /locus_tag="PK768_06340"
     CDS             complement(579..1295)
                     /gene="rsmG"
                     /locus_tag="PK768_06340"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQA60626.1"
                     /translation="MKNFADFLRKEAEKQHIFLSEDNIRQYDHFRTQLLKWNEKFNLT
                     NITEPEEFASKHIIDSLMLCKLGDIMTGARLIDVGTGPGIPGLVVKIYRPDIKLSLLE
                     SVGKKTMFLRWIVNDMAIADAEVINDRAENIAHDPAYREKFDVAVARAVAALNTLCEL
                     CLPFVKVGGTFIAMKGKSPEDELELAENALDILGGRVTEIQRYSLDKNVTRSLILVSK
                     LGESPSKYPRRPGVPVRKPL"
     gene            complement(1292..3174)
                     /gene="mnmG"
                     /locus_tag="PK768_06345"
                     /pseudo
     CDS             complement(1292..3174)
                     /gene="mnmG"
                     /locus_tag="PK768_06345"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
     gene            complement(3185..4573)
                     /gene="mnmE"
                     /locus_tag="PK768_06350"
     CDS             complement(3185..4573)
                     /gene="mnmE"
                     /locus_tag="PK768_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQA60627.1"
                     /translation="MKILESSDTIAAISTAIGEGGIGIVRLSGPESFDIARRIFKPRQ
                     KGVKDYEGRRLYLGDIVDFNDEIVDEVLVAFFKAPYTYTREDMVEINCHGGLAAQKKV
                     LRLVLDAGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRAKTDKALSLANRQLSGSL
                     SKKVNNIRQKLLDVLAHIAANIDFPDEDIPDADIAHIAGEISDAREMLSDLLKSISAG
                     RIIREGLATLILGNTNVGKSSLLNALLDEERAIVTDIPGTTRDIIEEYIDIKGIPIKI
                     IDTAGIRETSDVVEKIGIQRAMKYLEEAELVLLMIDVSRELTRDDKNLIQLIKDKTSI
                     AVINKVDLPAKIDEEELMEVLPTDRIVKISALKQEGIQELKDVIYELVTSKMEVSEEG
                     LIGAGERQRKVVETARDFLDSALNAINDFVPIEMVELDIRDAWRKLGEITGDTVTEDI
                     ITTVFQKFCIGK"
BASE COUNT         1076 a         1256 c          971 g         1413 t
ORIGIN      
        1 caatggtaga ttggctttta ccaagcttca ttgccaactg ttcctgggtc acgccgcact
       61 cctggattaa ttgccggtat ccttcggctt cctcgatgaa attcaaatct tccctttgga
      121 tgttctctat cagggccagg attgccgaat ccatgtcttt tgcatctttg attatggctg
      181 ggatttcctt gagccctgcc cgtttgcatg cgcgccaacg gcgttcaccg gcaatgattt
      241 catagccgct gcggcccctt cgccttacga caatgggctg gagaactccg taattcttaa
      301 tagagttggt gagctcgtcc agggtttcgt cattgaacgt cttcctgggc tggtatgcat
      361 taggctgtat ctcatcaatc ggtatattta caacattcaa tttagaccta ggaggccccc
      421 acatatcagt ccccccttaa tcaatatatc tcatatgata atatttagaa atgccgaaac
      481 ggctctatta ctctattcta tattatccgc atatttcctc ctttacaggc cctaaaacca
      541 tttgccataa taccctgtcc tttgacatct ttctccaact acaggggttt cctgacggga
      601 acaccgggcc tgcgaggata tttggatgga gactccccta gctttgagac aagtattaat
      661 gaacgtgtca catttttatc taaggaatac ctctgtattt cagttaccct gccccccaaa
      721 atatcaaggg cgttttctgc cagctctagt tcgtcttccg gcgatttgcc cttcatggca
      781 ataaaagtgc cgcccacttt gacaaagggc aggcagagtt cgcaaagggt attcaaggcg
      841 gccaccgccc tagcaacggc cacatcgaac ttctccctgt aagcgggatc atgcgctata
      901 ttttccgccc tgtcgtttat gacctctgca tcggctatcg ccatgtcatt cacaatccat
      961 cttaaaaaca tggttttttt accaacggat tcgagtaaac ttaactttat atccggcctg
     1021 tagattttta cgacaaggcc gggtatgccg gggccagtcc cgacatctat tagccgggca
     1081 cccgtcataa tatcccctaa cttgcaaagc atcagggaat cgattatatg cttgctggcg
     1141 aattcctcgg gttcggttat atttgtgaga ttaaacttct cgttccactt caacagctgg
     1201 gtcctgaaat gatcatattg gcggatattg tcctcgctta ggaaaatatg ctgtttttcg
     1261 gcttcctttc gcaaaaaatc agcaaagttt ttcatgatct ggctttcctt ctcttcgttt
     1321 ctagataaat catgagcacg gtgatatcag aaggatttac tccggatatt ctggatgcct
     1381 gaccgatgga catgggcttt agctttttca atttctcctg ccctcaaagc tcaggccgta
     1441 aacttcatca taatccaggt cggcagggat tcttttgttc tccattttct taaactgctc
     1501 tacctgtgca atttgacgct taatatagcc ttcgtaagca atgctgattt caacctgctc
     1561 aataacatcc ttcggcaggg gctgtctatt ttcatccaaa attttcactt tgtcatagtc
     1621 cagctccggc cgcttaatta gttcagccaa agttgaaggc gtgtttattg gagccgtgtc
     1681 aagccgattt agaatctcat ttaccccggc agtgggggtt atcttggcgg attgcaagcg
     1741 ctctatctca tcatctatca tctttttctt ctcagtgaat tttctatacc tgtcatcact
     1801 tatgaggcct atcctgtgcc ccatgtccgt aaggcgcaaa tccgcattgt cctggcgcaa
     1861 tatcagtcgg tattcagcac gagaggttaa gaccctgtac ggctcattga cgccttttgt
     1921 cactaaatcg tcaattaaca caccgatata agcatctgag cggcttaaaa tcaagggttc
     1981 cctctccttt atcttcaatg ccgcgttgat acctgcaatc aaaccttggg cagcagcttc
     2041 ctcataaccg gaagtgccgt ttatctgacc cgcaaagtaa agcccctgta tggccttggt
     2101 ttctaaggtg ggctttagct gagtaggaat tatatagtcg tattcaatag cataaccgaa
     2161 tctcatgatt tttgcatttt ccaatccctt aatggaacgg gtcatctcta tttgaacatc
     2221 tgccggaagg ctcgtggaaa ggccctgcac gtacatttca ttagtgttga caccttcggg
     2281 ttctatgaag atctgatggc tgctcttgtc cttgaagttg actattttca tctctatgct
     2341 cggacagtac ctgggccctg cgccctttat ttctccggtg taaagggggg cccgatagag
     2401 attctcactt atgatcctgt gagtaagttc attggtatag ctaagccagc agggtacctg
     2461 ctgtcgagaa atctcttcag tcgcatagga aaagggaata atgacctcat cccccggctg
     2521 aacaagcatc ttcgaataat ctatagaatc cctgtgaatc ctggggggag ttcccgtctt
     2581 aaacctgccc agctcaaagc ccaggctctc cagggatttg gagagctcat tggcgggaaa
     2641 aagcccgctg ggcccgctgc tgtaactcaa atcgcctatg acaattctgc cccgcaggta
     2701 cacaccggtg gttataataa cggccttggc cctgtagaca cctccggttt tggtcaccac
     2761 tccggagact ctatcccctt caaccaggac cttcaccact tcttcctgaa ctatatccag
     2821 gttttcctgg ctttccaatg tatatttcat gctggctgaa tacgcccttt tatccaattg
     2881 ggcgcgaagg gcccggactg cgggcccctt cttggtattt aacatccgca tctgtatgag
     2941 agtcttgtcc gcatttattg ccatctggcc ccccagggca tctatctccc taaccaaatg
     3001 cccctttccg ggaccaccga tggagggatt gcaggccatc agagctatgc tgtccaaatt
     3061 tatggcaaag gccaccgtct ttaggcccag ccgggatgca gccagggccg cctcggaccc
     3121 agcatgcccg gcaccgacca cggccacatc gtagtttcct acattgtatt ccatgctttc
     3181 accctcattt gccgatacaa aatttttgaa aaacagtagt aattatatct tcggtcactg
     3241 tatcgccggt aatctccccc agctttctcc aggcatccct gatatccagt tccaccatct
     3301 cgatgggcac aaaatcattt attgcattga gggcgctgtc taaaaagtct cgagctgttt
     3361 caactacctt ccgctgccgc tccccggcgc cgattaagcc ctcttcgctg acttccattt
     3421 tagatgtcac aagttcgtat atcacatctt ttaattcctg gatgccctct tgtttcagtg
     3481 ccgaaatttt tacaatccta tccgtcggaa ggacttccat cagttcttcc tcatctatct
     3541 tggctggcaa gtcaactttg tttataaccg caatgcttgt cttatccttt atcaattgaa
     3601 tcaggttttt atcatcccgg gtcagctctc gggaaacatc aatcatcaaa agtaccagct
     3661 ctgcctcttc aagatatttc atggcccgct ggatgcctat tttttcaacc acatcacttg
     3721 tctcccgtat cccggcagtg tcgattattt taatgggtat tccttttata tcgatgtact
     3781 cttcaattat atcccgggta gtgcccggaa tgtcggtcac gattgccctt tcctcgtcga
     3841 gcagggcatt taagagggac gacttgccca cattggtatt gcccaatatc agcgtagcta
     3901 aaccttccct gatgatgcga ccggcactga tgctctttaa caaatcgctg agcatttccc
     3961 tggcatcgga gatttcccct gctatatgag caatatcagc atccggaatg tcctcatcgg
     4021 gaaagtctat atttgccgct atatgggcga gcacatcaag gagcttctgc cgtatattgt
     4081 ttaccttctt agacaggctt cccgaaagct ggcgatttgc aagggaaagg gccttatcgg
     4141 tttttgcccg gattatgtca attacagcct ccgcctggga taggtcgatt ctaccgttta
     4201 aaaaagccct ctttgtaaat tccccgggct cggctaatct cgctcctgca tctaatacta
     4261 gccgcaggac cttcttttgc gccgcaaggc ccccgtggca gttaatttcc accatgtctt
     4321 ctctcgtgta tgtgtaggga gccttaaaaa aggcaacgag gacttcgtct acgatttcat
     4381 cattgaaatc aactatgtcg cccaggtaca gtcttctgcc ctcataatct ttgacacctt
     4441 tttgccttgg cttgaatatt cgccgtgcaa tatcaaagga ctcgggtccc gaaagcctta
     4501 ctattccaat gccaccttcg cctatggcag tagaaatagc agctatagta tcagaacttt
     4561 ctaatatctt catgccttat caccgcttta aaaagcccca agctttacgc aatggggcta
     4621 cttagtttac ctactttaga gatatgacga cccttctgtt gggttccttg cctacacttt
     4681 ttgtcgtaac cctgggatct ttttgcaaag ccgtat
//
LOCUS       DAONUO010000150         4627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13360_length_4627_cov_4.009405, whole genome shotgun sequence.
ACCESSION   DAONUO010000150 DAONUO010000000
VERSION     DAONUO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13360_length_4627_cov_4.009405"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1703)
                     /gene="serA"
                     /locus_tag="PK768_06355"
     CDS             complement(132..1703)
                     /gene="serA"
                     /locus_tag="PK768_06355"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQA60628.1"
                     /translation="MRVLVCDDLAQEGIDILKQEFEVDVKTKLREDELLEIIPKYDAI
                     VVRSATKVTQKIIEKSNLKVIGRAGVGVDNIDVEYATKKGVLVVNAPEGNTIAASEHT
                     IALLMAPARNIPAAVMSLKNKNWDRKKFMGVELRGKTLGVVGLGRIGKDVAKKAMGLG
                     MNVIAYDPYVIKEHVENIHIQVVDLEAVIKEADFITFHVPVTKSTYHMINKERINMMK
                     DGVIIINCARGGIIDETALYEGLVSGKIAACALDVFEHEPPLDSPLIDLPNVIATPHL
                     GASTAEAQINVAVDVARDIIRALKGEMVKNPVNMISIRPEEYKVIKPFMELAEKMGSI
                     YTQLKNGRISEVEMIYSGKIARFDVKPITTAGIKGILTNILQFPANVVNAYLLAKERG
                     IKIIEKKVSKDQDAPGTVTMKISTDLGVGSITGTVFGNDEQRIVAVDDYKIDLIPEGF
                     ALMSFHRDRPGIVGKVGTLLGQNDINIAYMQLGRKSYRGEALIVLGVDEEIPENILDK
                     IRQIEDIADAVFIKF"
     misc_binding    complement(1857..2056)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2107..2394)
                     /locus_tag="PK768_06360"
     CDS             complement(2107..2394)
                     /locus_tag="PK768_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HQA60629.1"
                     /translation="MEDRETITLLGENGEEAEFEVLGVITVEDSDYAILVPIEEDDDQ
                     AYIFRIDADENGDEILSQVEDDEEFEMVCEAWETLCEHELELDDDFVDEED"
     gene            complement(2431..2868)
                     /gene="ruvX"
                     /locus_tag="PK768_06365"
     CDS             complement(2431..2868)
                     /gene="ruvX"
                     /locus_tag="PK768_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQA60630.1"
                     /translation="MRILGLDIGEKRIGVAISDELYFSANGLDVIERKNNGEDINKIA
                     EIVSRYNVVKIVVGLPKNMDGSVGPSGEEVKKYVNKLQNALEVEVDFWDERLSTVAAE
                     KTLIEADISRKKRKKVIDKMAAVFILQNYLDFKSKVDSRKELL"
     gene            complement(2884..3831)
                     /locus_tag="PK768_06370"
     CDS             complement(2884..3831)
                     /locus_tag="PK768_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQA60631.1"
                     /translation="MQYNKLGFTDLTVSKLCFGSLTISPLQADLSIDDGAELILYAME
                     MGVNFIDTAEFYRNYQYINRALKKAKGELIISSKSYAYTYEGMKESVEKARKEMDKDV
                     VDIFMLHEQESRLTLKGHREALDYLVDAKAKGIIRAIGVSTHTVEVVNAAADMQEIDV
                     IHPLINFKGLGIKDGTLHDMEVAIKKAHKNGKGIYAMKVLGGGNLINDKKHAFSYILS
                     FPHLDSVAVGMQSIDEVVSNVCIFEGKTVEPVIEERLKSNKRKLLVEDWCIGCGECVK
                     HCRHNALFLHNGRSEVISDACILCGYCAGYCPEFCIKII"
     gene            complement(3983..4225)
                     /locus_tag="PK768_06375"
     CDS             complement(3983..4225)
                     /locus_tag="PK768_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HQA60632.1"
                     /translation="MGETVRFKVEKDTDIDAREILKEVWKALEEKGYNPINQIVGYLL
                     SGDPAYITSHGNARNLIRKLERDELLEVLVKNFIEG"
     gene            complement(4257..>4627)
                     /locus_tag="PK768_06380"
     CDS             complement(4257..>4627)
                     /locus_tag="PK768_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQA60633.1"
                     /translation="NQEIDEIIKMKETINGINVVSSKTEAKTIDDLRKMSDTLRQKLK
                     SGIIVLGSVSGDKIIFVAAVTDDLTKKGYHAGNIVKNVAQIAGGGGGGRPNFAQAGGK
                     KLEKLDEALASVSKMIREIN"
BASE COUNT         1247 a         1086 c          674 g         1620 t
ORIGIN      
        1 agtctttttc tttaagcttt tcctcaatga gtcgaatttt ttgtcttttc tcctcttcag
       61 aaaccattaa aaccaccctt ggctgctatt ttgtctatag tatattgcaa ataatattca
      121 ttgtcaagga ctcagaactt aatgaaaact gcatcggcaa tgtcttctat ttgtctgatc
      181 ttgtctaaaa tgttttcagg aatttcttca tccactccca agactattaa agcttctccc
      241 cgataagatt tgcggccaag ctgcatatat gctatattaa tgtcattttg ccccaataag
      301 gttccaactt ttcctacaat gccgggcctg tccctgtgaa aggacatcaa tgcaaaacct
      361 tcggggatga gatcaatctt gtaatcatcc acagcaacta ttctctgttc atcatttcca
      421 aatacagttc ctgttatact tccgacaccc aaatcggtag aaatcttcat tgtaacggtt
      481 ccgggagcat cctgatcctt gctcactttt ttctcaatta ttttaattcc ccgttctttg
      541 gcgagtaagt aggcattaac aacgttagca ggaaattgta agatgtttgt caatatgcct
      601 tttatgcctg ctgtcgttat cggcttaaca tcgaacctag ctatcttgcc ggaatatatc
      661 atctcaactt cgctgattcg cccgttcttt agctgtgtgt aaattgagcc catcttttca
      721 gctaattcca taaatggttt aattaccttg tattcttcag gccgtatgga tatcatgttt
      781 accgggtttt tgaccatttc gccttttaag gctcttatta tgtctcttgc tacatcaact
      841 gcaacattaa tttgtgcctc agcagtagaa gcacccaaat gcggtgttgc tataacattt
      901 ggcaaatcta tgagagggct gtccaggggt ggctcatgtt caaatacgtc aagtgcgcat
      961 gcagcaattt ttcccgatac tagcccttca taaagggcag tttcatcaat gatgccgcca
     1021 cgagcacaat ttatgataat gactccatct ttcatcatat taatgcgttc tttgtttatc
     1081 atgtgatagg ttgattttgt cacaggaaca tgaaaagtta taaaatctgc ttctttaatt
     1141 accgcttcca ggtctactac ctgaatatgg atattttcca catgttcttt gattacatac
     1201 gggtcatatg caataacatt cattcccaag cccatggctt ttttagccac atcttttcct
     1261 atacggccca aaccaacaac accgagggtc ttgcccctaa gttcaacacc catgaatttt
     1321 ttcctatccc aatttttatt tttaagactc ataactgcag caggaatatt cctcgcggga
     1381 gccattaata atgcaatggt atgctcgcta gctgcaatag tatttccttc gggagcattt
     1441 actactaaaa cacccttttt cgttgcatat tcaacatcaa tgttatccac gcctactccg
     1501 gctctgccta ttacctttaa attgcttttt tctatgattt tttgtgtaac cttggttgca
     1561 cttctcacaa caatggcatc gtatttaggg attatttcca aaagctcatc ttctcttaat
     1621 ttggttttga catctacctc aaactcctgt tttaatatgt caataccttc ttgtgctaaa
     1681 tcatcacata ccagaactcg catattacct accccttctt ttcaaaatct tgttatgatt
     1741 tttcataatt gccaggtcta ttaatataaa taaactgtta taatttcaat cgaccgttct
     1801 cttttgacct ggaattaaat ataaacaaaa cttgatttaa caaaaaaacc gcccgtacaa
     1861 ggggcggatc ttaccgcgtt gccaccctat ttcggtttcc caaaataagc acaaccctca
     1921 ttttttataa cggtttttac cgtttgactc atcgtcaacg actccaaggc ggaaataatg
     1981 ggttattacc ggttcacacc agccaccggc tctctgaaaa taaaactcca ttaattttcc
     2041 tcttcacagt ctttccctta tttaaattat taacaattat aaattaggac ttgttatctg
     2101 tcaagcctaa tcttcttcat ctacaaaatc atcatctaat tccaattcgt gttcgcacaa
     2161 tgtttcccag gcttcacata ccatttcaaa ttcttcatca tcttccacct gagagagtat
     2221 ttcgtcacca ttttcatcag catcgatgcg aaatatatat gcttggtcat cgtcttcttc
     2281 aatgggaacc aatatagcgt aatcactgtc ttcaacagta ataactccaa gcacctcaaa
     2341 ttcggcttct tcgccatttt cgcccagcaa agtaatagtt tctcggtctt ccattttttc
     2401 aactccaatt cttattgaat taaagatatt ttatagtaat tctttcctgc tgtcaacttt
     2461 tgatttaaag tctaaataat tttgaagaat aaacactgct gccattttat cgatgacttt
     2521 ttttcttttt ttgcgggaaa tgtcggcttc aattaaagtc ttctcagcag ctacagtaga
     2581 cagcctttca tcccagaagt ctacttcaac ttctaatgca ttttgaagtt tattgacata
     2641 ttttttgact tcctctccac tagggcctac gctcccatcc atatttttcg gaaggccaac
     2701 tactattttt acaacattat atcttgaaac aatttcagca atcttattta tatcttcacc
     2761 attattcttt ctttctatta catccaaacc gttggcggaa aaataaagtt catctgatat
     2821 tgcgactcct attctttttt ctcctatatc caaacctaat atccgcactt agggttactc
     2881 cttttatatg attttaatgc aaaactcggg acagtacccg gcacaatatc cgcagagaat
     2941 acatgcatcc gaaattacct ctgaacgtcc attgtgaaga aaaagggcat tgtgccgaca
     3001 atgctttaca cactccccgc aaccgatgca ccagtcttct accaatagct tccttttgtt
     3061 actttttaat ctttcttcta taacaggttc tactgttttt ccttcaaata tgcaaacatt
     3121 tgatacaact tcatctatag actgcatccc taccgctact gaatcaagat gtggaaatga
     3181 taatatgtaa gagaaggcat gttttttatc gtttattaga ttcccgcctc ctaaaacctt
     3241 catggcatat atacccttac catttttatg ggcttttttt atggcaactt ccatgtcatg
     3301 caaagttcca tctttaatac ctaaaccttt gaagttgatt aatggatgga taacatcaat
     3361 ttcctgcata tcagctgccg catttaccac ttccaccgta tgagttgaaa cgcctattgc
     3421 ccttattata ccttttgcct tggcatcaac caaataatcc agagcttctc gatgtccctt
     3481 aagtgttaat cgactttctt gttcatggag cataaaaatg tctacaacat ccttgtccat
     3541 ttccttgcgt gccttttcta cactttcctt cattccctca tacgtatagg cataagattt
     3601 tgaagatatt atcagttccc ctttagcttt cttcaaggcc ctgtttatat attgatagtt
     3661 tctatagaat tcggcggtat ctataaaatt aacccccatt tccatggcgt aaagaataag
     3721 ttctgctcca tcatcaatag ataaatcggc ttgaaggggg cttattgtca ggctgccaaa
     3781 acacagctta gacactgtca aatcagtaaa cccaagtttg ttatactgca taaattgttg
     3841 acctctttta acatgttttt atttacataa cacgataaaa aaagtatcag caggcaatag
     3901 atcaaaataa atttacatac acaataatgc atgagatcgc ctgctgattg tcattcatat
     3961 tcctatctca gatcttgtca gattagcctt cgataaaatt ctttacaagt acttctaata
     4021 attcatcccg ctcaagcttg cgaatgagat ttcttgcatt gccatgactg gtaatatatg
     4081 cagggtcacc ggataaaagg tagcctacaa tctgatttat ggggttatat cctttttctt
     4141 ccagggcctt ccagacttct tttaatatct cacgggcatc aatatcggta tccttctcca
     4201 ctttaaatct aaccgtttca cccatgttct gccccattgt atctccctcc atcgttttaa
     4261 ttgatttccc ttatcatttt tgaaaccgag gcgagagcct catcgagttt ctcaagtttc
     4321 ttaccaccgg cctgggcaaa gtttggcctt ccccctccgc ctcctccggc aatctgggcc
     4381 acattcttta caatgttacc ggcatgatag cccttcttag ttaaatcatc tgtcacagcg
     4441 gcaacaaata ttattttgtc accactaaca ctgcctaaca cgatgatgcc ggattttagc
     4501 ttttgtcgca gtgtatcgct cattttcctt aaatcatcta tagtttttgc ctcagtcttg
     4561 cttgatacta catttatacc gtttatagtc tctttcattt ttataatttc atcaatctct
     4621 tgattaa
//
LOCUS       DAONUO010000151         4621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13385_length_4621_cov_3.691196, whole genome shotgun sequence.
ACCESSION   DAONUO010000151 DAONUO010000000
VERSION     DAONUO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13385_length_4621_cov_3.691196"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..752
                     /locus_tag="PK768_06385"
     CDS             63..752
                     /locus_tag="PK768_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA60634.1"
                     /translation="MKDIRILVVDDEKRIVDLVRLYLEREGYVVDEAFNGEAALDMIS
                     KRSYDLIVLDIMLPVIDGWTVCKELRKKYDTPVIMLTARGDEFDKVLGFELGADDYVV
                     KPFSPRELTARVKALLRRTATRKDEETDALVFPDLFIDPSARVVKVNDKEITLTPKEF
                     DLLYFLAKNKDKVFTREKLLEEVWGYDFYGSLRTVDTHIKQLREKLGRSKAASYINTI
                     WGVGYKFEVEE"
     gene            749..2470
                     /locus_tag="PK768_06390"
     CDS             749..2470
                     /locus_tag="PK768_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA60635.1"
                     /translation="MNIKKSIISKLWLYMTVLAIVTLLFSGLVLSSVFEDFYFNMRKN
                     EMINEGQQLLSLILQGVNPSELLDISKFINAHAVLIDRQGLIQASSNILKLEGMAIGA
                     KELAEVLKGNTVVHKGHIAQFNAPMLTVALPIKSELGVVGGLILYSPMSTIENTIWQI
                     RRLILLAAAITVMISTGLSFVLSRTVSKPLVQMKKAAEEMAKGKFDNKVNVDTDDEIG
                     TLAKTMNYLSDALNENINALNQEKNQLQNVLLSMTDGVVTFDENGQVIMANPQAIEII
                     SDVSEDASTQFDVLKDFLEQVKESGEYVKREIKLKGKTISVRMAPLLDDHMKLWGVLA
                     VLQDVTRERKLEDLRREFLGDVSHELRTPLTYLQGYTEALLDNMVKNEEERSRYLNII
                     LEETLRLRRLVDELLDLSHIEAGHLDIKKNSISIESIVEKVSKKVHPLCDSKKIELEI
                     DIQGDIPLIVADEDRIEQVLINLVDNAVRYSPKDSKVTVKVRPSDEGVIVTVKDSGQG
                     IPPEELPFVWERFYKVDKSRERKKSGTGLGLAIVKKIVELHNGRVWAQNCDEGGAEFS
                     FYLPSSV"
     gene            complement(2511..3632)
                     /gene="ald"
                     /locus_tag="PK768_06395"
     CDS             complement(2511..3632)
                     /gene="ald"
                     /locus_tag="PK768_06395"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HQA60636.1"
                     /translation="MIVGVVKELKEQENRVAITPAGVDALTACGHKVLIQKGAGLGSG
                     FSDESYRAAGAEIMNDASDVWENCEMMVKVKEPHESEYKYLRPDLVFFAYLHLAADYN
                     LTEGLMDSGITAIAYETVQRSDRSLPLLIPMSEVAGRMAIQEGAIYLEKTRGGKGILL
                     EGVPGVTPASVVIVGAGTVGTGAIRRAMGLGARVTVIDINVDRLRYLEDIFMGRIETL
                     YSNRYNLSKALKYADLVVGAVLIPGAKTPKLITEEMVKKMQPGSVIVDVAIDQGGCVE
                     TIERPTTHANPVFVKHGVIHYAVSNIPGAVPRTSTLALTNATLPYLLEMAKKGWKQAI
                     QDDEALARGVNVMNGKITNKAVAEAHNLQYHPLEEVMKE"
     gene            3808..3966
                     /locus_tag="PK768_06400"
     CDS             3808..3966
                     /locus_tag="PK768_06400"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQA60637.1"
                     /translation="MFNIGTSELLIILALSLIILGPGKLPEVGQALGKALGEFKRAVR
                     ETRDDAEK"
     gene            complement(4400..>4621)
                     /locus_tag="PK768_06405"
     CDS             complement(4400..>4621)
                     /locus_tag="PK768_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60638.1"
                     /translation="SYRAHMGKANSSCDRASGGVIIAAKINIAIIVDFRYLLKVSPLI
                     IPSIAAITIRRGICDMMAKAIVMFMKNDV"
BASE COUNT         1372 a          895 c         1022 g         1332 t
ORIGIN      
        1 taagattaga tgtggataag ctttcattaa agttactgct attgccgttt ggggggctga
       61 gtgtgaaaga tattagaatt ttggtagttg atgatgaaaa gcgcatagtg gatttggtta
      121 ggttgtatct tgagcgtgaa ggttacgtag tagatgaagc ctttaacggt gaagctgcct
      181 tagatatgat atcgaaaagg tcttatgacc tgatcgtcct ggatataatg ctgccggtca
      241 tcgatggctg gacggtatgt aaggaattgc gcaaaaagta cgataccccc gttataatgc
      301 ttacagcgag aggggatgag ttcgataagg ttttgggatt tgaactgggt gctgacgatt
      361 atgtggtaaa acccttcagc cctagggagc ttacagcaag ggtaaaggca ctgttgagac
      421 gcactgcaac aaggaaggat gaggaaacgg atgccctggt ctttccggac ctttttattg
      481 acccgtctgc tagagtggtc aaggtgaatg ataaggaaat tactcttact ccaaaagaat
      541 ttgatctctt atattttttg gctaaaaaca aggataaggt ttttaccagg gaaaaattgt
      601 tggaggaagt ttggggatat gatttttatg gcagcctcag gacagtagat acccatatca
      661 aacagcttag ggaaaaactt ggtaggagta aagcggcctc gtacataaac actatatggg
      721 gcgttggtta taaatttgag gtggaagagt gaatattaaa aagagcatta tatcaaagtt
      781 atggctttat atgacagttt tggccattgt cacacttttg ttctcaggac tggtcttgtc
      841 cagtgttttt gaggactttt attttaatat gcgaaaaaac gagatgataa acgagggaca
      901 gcagttattg tcccttattt tacagggagt aaacccatcc gagcttcttg atataagtaa
      961 gttcatcaat gcccatgcag ttttaataga caggcagggc cttattcaag caagctcaaa
     1021 tatactaaaa ttagaaggta tggccattgg agctaaagaa ttggccgaag tattaaaggg
     1081 caatacggtg gtacataaag gccatattgc ccagtttaat gctccaatgc taacagtggc
     1141 attgcccata aaatcggaac tgggagtggt gggtggcctt attttatact cacccatgtc
     1201 taccattgaa aacaccatat ggcagatcag aaggttgatt ttgctggcgg cagccataac
     1261 cgtaatgatt tctacaggac tgagttttgt tctttcaagg actgtgtcaa aacccctggt
     1321 acagatgaaa aaagctgccg aggaaatggc aaaggggaaa tttgacaaca aggtcaatgt
     1381 agacacagat gatgaaatcg gtaccctggc caaaaccatg aactatctat cagatgctct
     1441 aaatgaaaat atcaatgcct taaaccagga aaaaaatcaa cttcaaaatg tacttctaag
     1501 tatgacagat ggcgttgtga cctttgatga aaatggtcag gtcatcatgg caaatccgca
     1561 ggcaatagaa atcatatccg atgttagtga agatgccagc acccagttcg atgttttaaa
     1621 agatttcctt gagcaagtca aagaaagtgg tgagtacgtt aagcgggaga taaaattaaa
     1681 agggaaaacc ataagtgtga ggatggcgcc ccttttagat gatcatatga agctttgggg
     1741 tgtattggcg gtgcttcaag acgtgaccag ggaaagaaag cttgaagatt tgagaaggga
     1801 gtttttagga gatgtatccc atgaattgag aacgcccctt acctatcttc agggatacac
     1861 cgaagccttg ttggataaca tggtaaagaa tgaagaagag cgatccaggt atttaaatat
     1921 tatccttgag gaaaccctga gattgcgccg cttggtagat gaattattag acctctcaca
     1981 tatagaagca ggccatttag acataaagaa aaatagtata tcaattgagt ctatagttga
     2041 aaaggtatcg aaaaaggttc atcccctttg cgacagcaag aaaatagaac tggaaattga
     2101 tattcaaggg gatataccct tgattgtggc tgacgaagac cgcattgaac aggtcttaat
     2161 caatctagtg gacaacgctg tgaggtattc tcccaaagac agcaaggtaa ctgtgaaagt
     2221 tcgaccttca gatgaaggtg tgatcgtcac tgtaaaagac agtggtcagg gcataccccc
     2281 tgaagagctg cccttcgttt gggagagatt ttacaaagtg gacaaatccc gtgaacggaa
     2341 aaaaagtggc accggtcttg gcctagcaat tgtgaagaag attgtagaac ttcacaatgg
     2401 ccgtgtttgg gctcaaaatt gcgatgaggg aggagcggaa ttttctttct accttccgag
     2461 ttcggtgtaa aaatacaagt ctcccgattg ggagacttgt aatataaaag ctattctttc
     2521 ataacctctt ccagtggatg gtattgaagg ttgtgagcct ctgcaacggc tttattggtt
     2581 atcttaccat tcatgacatt tacacccctg gccagggctt catcatcttg tatagcctgt
     2641 ttccagcctt ttttggccat ttcaagcaaa tagggtaagg tagcattggt cagagccagg
     2701 gtagaggtgc gcggaacagc tcccgggata tttgacactg catagtgaat aacgccgtgt
     2761 tttacaaaaa cgggattagc atgggtagtg ggccgttcaa tagtctccac acagccgccc
     2821 tggtcgattg ccacatcgac tatgacgctt cccggttgca tttttttaac catctcttca
     2881 gtaatcaatt taggtgtttt cgcacccgga atcagaactg ctcctacgac aagatctgca
     2941 tattttaatg ccttggacag gttgtaacga ttggaatata gggtttcaat tctgcccata
     3001 aagatatctt caaggtagcg tagcctatca acatttatat cgatgacagt aacacgggct
     3061 cccagaccca ttgcccttcg gattgcaccg gtacctacag tgcctgcgcc cacaattact
     3121 acggatgcag gagttacacc gggcacacct tctagcaaaa tacctttacc tccacgagtc
     3181 ttttcaaggt atatagcacc ttcctggatg gccatacggc ctgccacttc actcataggt
     3241 attaagagag ggaggcttct gtcactgcgc tgaacggttt cgtaagctat ggctgtgatg
     3301 cctgaatcca tgagaccctc cgtcagattg tagtcagctg ccagatggag atatgcaaaa
     3361 aagactaagt caggtcttaa atacttatat tcagattcat gaggttcttt aaccttaacc
     3421 atcatttcac agttttccca aacatcagag gcatcgttca tgatttctgc tcctgctgct
     3481 ctgtaacttt catcggaaaa acccgaaccc aatcctgcac ctttttgaat caatacctta
     3541 tggccacacg ctgtaagtgc atctacacct gcaggagtaa tggcaactcg attttcttgt
     3601 tcttttaatt cttttacaac accaacaatc atctttttgc ctccttacga attaaagcat
     3661 tcaaaacaaa tatatagaag agtatatcaa aaaaataaaa attgcaccag tatttttttg
     3721 ccttcaggta attattgaga atatttttag tttatcattc caatactata tttatagaca
     3781 aaaaatcctt tatcgaggtg acctggcttg ttcaacatcg gaacgtcaga acttctaatc
     3841 attttggccc tgtctttaat aattctcggt cccggcaagc tgcctgaagt cggtcaagct
     3901 ctaggaaagg ccttaggcga gtttaaacgc gcagttcgtg aaacaagaga tgatgctgag
     3961 aaataatgga ggacaaaaag ctgcccatat ccttacacct aaaagaactc agattgagac
     4021 ttttgttttc cttagcctcg gtagtagttt tttctttaat tagttttatg tgctggccaa
     4081 atataataga tgcaattcgc ttaaaagaca taaacttaat tttcatcagt cctctagagg
     4141 cttttttgac aagaattaaa gctgctgtgt tttgcgggat attgctttcc atgcctatca
     4201 ctttatatca aatctgggaa tatataaaac ctgccctgac taaaagggag cgaaaacttg
     4261 ctatcgtact tgtaacggct tctagtcttc tactgtattt aggattatgt ttcagtgtat
     4321 gtgtagttac gccaatgatt gcaaatttct tcctaaaatc agcccaatcg gctttaactc
     4381 ccctcatttc tgtgtcaaat tacacgtcat ttttcataaa catgacaatg gcttttgcca
     4441 tcatatcaca aattcccctt cttattgtaa ttgcagcaat gctaggaatt ataagcggtg
     4501 agactttaag taaataccga aaatcaacga ttatagcgat atttatttta gcagctatta
     4561 ttacgccacc tgatgccctg tcacaactgc tgttggcctt gcccatgtgg gccctatatg
     4621 a
//
LOCUS       DAONUO010000152         4611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13416_length_4611_cov_4.012730, whole genome shotgun sequence.
ACCESSION   DAONUO010000152 DAONUO010000000
VERSION     DAONUO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13416_length_4611_cov_4.012730"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PK768_06410"
     CDS             complement(<1..328)
                     /locus_tag="PK768_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HQA60639.1"
                     /translation="MMKTLFIVNPVAGRNNCYRIWNDIKAHIDFPHEVILTKGPGEAK
                     YLAESAVKQGFKRMVAVGGDGTVSEVVNGIVGTDVELGIIPAGSGNDFIKALNIPQRP
                     LDALAVI"
     gene            complement(357..773)
                     /locus_tag="PK768_06415"
     CDS             complement(357..773)
                     /locus_tag="PK768_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQA60640.1"
                     /translation="MFLFKSESAVSQDKIDTILGKDAKLTGEIKTSGLMRIEGSFQGD
                     IECERDLTITNSANVEGKIKAKNAIIAGTFKGNIFLDGKLEIKNTGKVIGDVKIGTLV
                     VEDGAVFDGKCEMIGDAKDKNLKPTTKDAKFQTNPT"
     gene            complement(790..1701)
                     /locus_tag="PK768_06420"
     CDS             complement(790..1701)
                     /locus_tag="PK768_06420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQA60641.1"
                     /translation="MNESNHKFTMMIVPHSGKSTFSISVSILTLKIIGGLLATVMIVL
                     LVTSLIFFSSYAKLKQDTQELKTVAEDYDVLQRQLQLFLQKTNSIEKKMQELEQLDND
                     LRGLLKDDPALKDVLEKKDSQASLQRSTLSSRGSIDRQQAIRQLLLIEQTIPEQQQSL
                     EELKEVIIKRADRLSHTPSIYPVKGKITSRFGYRRSPFGRRTEFHDGLDIAAAYGTEI
                     VATADGVVTFTGYKSGYGRTVTINHGYGYETSYCHNSSILVKTGQQVKKGQVIAKVGN
                     TGRSTGPHLHYMVKVNGQLKDPAEFLN"
     gene            complement(1809..2315)
                     /locus_tag="PK768_06425"
     CDS             complement(1809..2315)
                     /locus_tag="PK768_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HQA60642.1"
                     /translation="MDYMTIINNFTAANIEELLLASLVLSVIMLIAFLSINIKLAKVT
                     KKYNELTEGAEGINLEELILSQGKRLNQYQSELNELKASFYQLEEFSRETISKVYFKR
                     YNAFPDMGSDLSFSVLLVNAKNTGVLLTSIYGRDENRVYLKPIVNGNSDYVLSPEERE
                     ILNKCISS"
     gene            complement(2380..3072)
                     /locus_tag="PK768_06430"
     CDS             complement(2380..3072)
                     /locus_tag="PK768_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HQA60643.1"
                     /translation="MLGTIVNAATIIIGTMLGNSLKGRFSENIKQTVMHGLSLTVMLI
                     GFSMAIGTENMLIVTLSMVIGGIIGELLKIEDRLNSFGRKLETRFSSEGGDFTRAFVT
                     ASLVYCVGAMAILGSIQSGLEGDHSILFVKSILDGVSAVVFSSSMGIGVAFSALPVFI
                     YQGAITLAASYIKAFLTNNIITEMSATGGLLIFGIGLNMLGINVNIKVGNLLPAIFVA
                     VLLAAAFARAPI"
     gene            complement(3231..4073)
                     /locus_tag="PK768_06435"
     CDS             complement(3231..4073)
                     /locus_tag="PK768_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQA60644.1"
                     /translation="MSKRGLGRGLEALIPIVEKDEENVQEIDIKKIVANEKQPRRGFD
                     ESKLEELADSMKQHGVLQPVILRKKGNKYELVAGERRWRAAAKAGIEKIPAIVRELND
                     SDVMEIALIENLQREDLNPIEEAMAYKSLMDDFGLTQEELSKRVGKSRSQIANTVRLL
                     NLDKEIQDLVYENKLTAGHARALLSIQDSKERLKLANMISKEALSVRQTEQLVKKLVD
                     ERSKAKKHKPKEINPVIIDVTERLQRTLGTRVKIKGNEKRGKIEIEFYSSEELERILE
                     IIAE"
     gene            complement(4066..>4611)
                     /locus_tag="PK768_06440"
     CDS             complement(4066..>4611)
                     /locus_tag="PK768_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQA60645.1"
                     /translation="NIVETEYKNLYLLPSNIQLAGAEIELVPAISREYKLKNAIDDVK
                     NKYDFIIIDCPPSLGLLTLNALTAADTVLVPIQCEYYALEGLGQLMNTINLVQKHLNK
                     NLEVEGVLLTMFDARTNLSIQVVDEVKKYFKNKVYRTIIPRNVRLSEAPSHGKPIINY
                     DSKSRGSEVYMDLAKEVLGIE"
BASE COUNT         1080 a         1138 c          893 g         1500 t
ORIGIN      
        1 taatcactgc aagggcatcc aggggccttt gaggaatgtt taatgccttt ataaagtcgt
       61 ttcccgagcc cgcaggaatt atccccagct ctacatcggt gccgactata ccgtttacga
      121 cttcagaaac ggtcccgtcc cctccgaccg ccaccattcg cttaaaacct tgtttaacgg
      181 cgctttcggc caaatatttt gcttcaccgg ggcccttggt gaggataacc tcgtgaggaa
      241 aatctatatg agcttttata tcgttccata ttctgtaaca gttgttacgt ccggctacag
      301 gatttactat gaataatgtc ttcatcattt ctccctttat ctaaaacaaa cccaactcat
      361 gttgggtttg tttgaaactt tgcatccttt gtcgtaggtt ttaagttttt gtccttggca
      421 tcaccgatca tctcgcactt gccatcgaaa acggccccat cttcaactac cagcgtccct
      481 atctttacat cacctattac tttgccggtg ttttttattt ccaatttccc atctaaaaaa
      541 atatttccct taaaagtacc ggcaattatg gcatttttgg ccttaatttt accttccaca
      601 tttgcactgt tagtaatggt caaatccctt tcacattcta tgtcaccctg gaagctgcct
      661 tcgattctca tcaggcccga tgtctttatt tcaccggtta atttagcatc cttgccaaga
      721 atggtgtcta tcttgtcctg acttacggcg gattcacttt taaataagaa caacatgtta
      781 tctcctctct caattcaaaa actcagcagg gtcttttaac tgcccgttta cttttaccat
      841 gtagtgcaga tgagggcctg tgctcctgcc tgtgtttccc acctttgcta taacctggcc
      901 ttttttcacc tgttggcctg tctttacaag aatgctggaa ttgtggcaat aagatgtttc
      961 gtatccgtag ccatgattta tggtcactgt cctgccgtat ccgcttttgt atcctgtaaa
     1021 agtcaccaca ccatctgctg tagcaactat ttccgtcccg taagctgctg cgatgtctag
     1081 gccgtcgtga aattccgttc ttcggccaaa gggggatctc ctatatccaa acctggatgt
     1141 gattttaccc ttgacgggat aaatagaagg tgtatgtgat aagcgatcag ccctttttat
     1201 gatgacttcc tttagctctt caaggctttg ctgctgctca ggaatcgttt gctctatcag
     1261 cagcaattgc ctgattgcct gctgcctgtc aatactgcca cgacttgaaa gtgttgacct
     1321 ttgtaagctt gcctggctat cctttttctc aaggacgtct ttaagagccg gatcgtcttt
     1381 tagcagtccc cttaaatcgt tgtccaactg ctctaactct tgcatcttct tttctatact
     1441 gttggtcttt tgtaaaaata gctgcaattg tctttgtagc acatcgtagt cttccgctac
     1501 cgttttaagt tcctgcgtgt cctgctttaa cttggcgtag ctgctgaaaa aaatcaaact
     1561 tgttacaagc aaaactatca taaccgttgc caacagacca ccgataattt tcaaggtcag
     1621 aattgacaca gaaatagaaa aggtggattt gcctgagtga ggaacgatca tcattgtaaa
     1681 cttgtgattg ctttcgttca ctccttgaac caccacctcg tatatgtaat tatggacaat
     1741 atatccaata tataattcgc catgcagtca aaaaatcctt cttgcccggg gaaattaagt
     1801 tttggtcctc agctcgagat acatttgttt agaatctctc tttcctccgg cgaaaggacg
     1861 tagtcgctat ttccgtttac tatgggcttt aagtacaccc ggttttcatc ccgtccgtat
     1921 atactggtca gcaggactcc cgtgtttttt gcattgacca agagcacgga aaagcttaaa
     1981 tcactgccca tatctggaaa agcattgtac cgcttaaaat atactttgct gatagtttcc
     2041 cggctgaatt cttcaagctg ataaaatgaa gcttttaact catttagttc tgattggtac
     2101 tgattgagtc ttttcccctg gctcaaaata agttcctcaa ggtttattcc ctcggcacct
     2161 tccgtgagtt cattgtattt tttggttacc ttggccagtt ttatgtttat gctcaaaaag
     2221 gcaattagca ttatcaccga taataccaga gaagccagca atagttcttc gatattggca
     2281 gctgtaaagt tatttattat tgtcatgtaa tccatactat taagattccc ccatcatcgt
     2341 ttgatgcaga acgattgttt ggtatttttc ttgtttttct taaattggcg cacgcgcaaa
     2401 ggctgccgcc aaaagaacag ccacaaatat ggcaggcagc aggttgccta cctttatatt
     2461 tacatttatt cctaacatgt ttaaaccgat gccgaatatc agtagcccgc cagtagccga
     2521 catttcagtt atgatgttat tagtgaggaa tgcctttatg tatgaggcgg ccagggtaat
     2581 cgctccctgg tatatgaaaa ccggaagtgc tgaaaatgcc acgccaattc ccatgcttga
     2641 tgaaaacacc accgccgaaa ctccgtcaag gatggacttt acgaatagta tgctgtggtc
     2701 accttcaagg ccgctctgta tcgaacccaa tattgccatg gcgccaacgc agtacaccag
     2761 gctcgcagtg acgaaggccc ttgtaaagtc tcccccttca ctggaaaagc gtgtttccag
     2821 cttcctgcca aagctgttga gcctgtcttc aatcttcaaa agctccccaa ttatgccgcc
     2881 tattaccatg ctgagggtca ctattaacat gttctctgtg cctatggcca tggaaaagcc
     2941 tatcagcatg accgtcaggc tcaggccgtg cattacggtt tgctttatgt tttcactgaa
     3001 gcggcccttt aatgagttgc ccagcatcgt ccctatgata attgttgcag cgtttacaat
     3061 ggtcccaagc aatgtcctct ctccttaatg cgaaagattt tctctacatt ttatcacgag
     3121 ttcggtgatg cgtaaagagc ctatgctgct cctggaatct atgctctgtt gactcatctt
     3181 ttgtgcggga acaacttcac aatacggtgc ttgtttcacg tgaaacattt ctattcggca
     3241 atgatttcca ggattcgctc cagttcctca ctggagtaaa actctatttc gatcttgccg
     3301 cgcttctcgt tgccctttat ttttaccctg gttcccaggg tcctttgcag tctttcggtc
     3361 acgtcaatta taaccgggtt gatttcttta ggtttatgtt tttttgcctt gctccgttca
     3421 tcgacaagct tttttaccag ttgctcggtc tgtctcaccg ataaggcttc cttgcttatc
     3481 atattggcca gctttaacct ttccttgctg tcctggatcg acaaaagggc cctggcatgg
     3541 ccagcggtta atttattctc gtaaactaag tcctgtattt ccttatctag attaagcagc
     3601 cgaacggtgt ttgcaatctg cgacctgctt ttgccaaccc tctttgacag ctcttcctgg
     3661 gtgaggccaa aatcgtccat caggcttttg tacgccatcg cttcctcgat gggattcaag
     3721 tcctcccgct gcaggttttc aataagggct atttccatga catcagaatc gttaagctcc
     3781 ctaacgatgg caggtatttt ctcgatgccg gccttggcag ccgccctcca cctgcgctca
     3841 cctgccacca gttcgtactt gttgcccttc tttctcaaga tcaccggctg cagtacccca
     3901 tgctgtttca tggagtcagc aagctcctcc agttttgatt cgtcaaaacc ccttctgggt
     3961 tgtttttcat tggcaacgat cttttttatg tctatttcct gtacattttc ttcgtctttt
     4021 tcaacaatgg gaatcaaagc ttccagacct ctaccgagtc cccgtttact caattcccaa
     4081 tacctccttt gctaaatcca tataaacttc ggagcccctt gatttagagt cgtaattaat
     4141 aatgggtttg ccgtggcttg gtgcttcact taggcggaca tttcggggaa tgatggtcct
     4201 gtaaaccttg tttttgaagt acttctttac ttcatctacc acctggatag ataagttagt
     4261 cctggcatca aacatggtca aaaggacccc ttccacctcc aggttcttgt ttaggtgttt
     4321 ttgcaccaaa tttatggtgt tcatgagctg gcctaatcct tcaagggcat aatactcgca
     4381 ttggatcggc accaggacgg tatctgctgc agtcaatgca tttaatgtca agaggccaag
     4441 agacggagga cagtcgatta tgataaaatc atacttattt tttacatcat caatggcatt
     4501 ctttaatttg tactccctag atatggcagg aactaactct atctctgcac cagccagttg
     4561 aatgtttgag ggcagtaagt atagattctt gtactctgtc tctacaatgt t
//
LOCUS       DAONUO010000153         4603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13450_length_4603_cov_3.705145, whole genome shotgun sequence.
ACCESSION   DAONUO010000153 DAONUO010000000
VERSION     DAONUO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13450_length_4603_cov_3.705145"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PK768_06445"
     CDS             complement(<1..230)
                     /locus_tag="PK768_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQA60646.1"
                     /translation="MIMKIARALHKRTKGDKGFTLIELIVVIAVLGVLATLIIPKVVG
                     VKSEAETKAMEANEKIIKNALERYYLKHGKYP"
     gene            complement(280..1494)
                     /locus_tag="PK768_06450"
     CDS             complement(280..1494)
                     /locus_tag="PK768_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQA60647.1"
                     /translation="MTSIYRYTGRNNEGKSVKGMYKAESESDVAAMLRAKGIYPVTIK
                     KEETGDILEILKNLTKKANPKDLAVYCRQFSAVIGAGIPIVKSLEILQLQGISPVLVK
                     ATQNAREDIISGLSLGQAFRNNADVFPEIFVNMVEAGEISGSLNNALERLAEHFEREN
                     DIINKVKSATTYPIILSSTAIVVVILIVTKVLPIFAGMFESYGAALPIPTRILLVISD
                     FLSRFFWILVLALVLAFLLFIKIRSTPEGRLLLEKIYYNIPFFGPIVTKLILARFSRT
                     LSTMILNGIPLLDAVDTMNRVIESETLKIECNKIKESIQKGAGISEAAASGNFFPPLF
                     VEMTAIGEETGSLTEMLNKIADYYDREVKDVLNRLSTILEPMIIVVMAVVIGFIVISI
                     ILPMFEMMRFVG"
     gene            complement(1539..2597)
                     /locus_tag="PK768_06455"
     CDS             complement(1539..2597)
                     /locus_tag="PK768_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQA60648.1"
                     /translation="MVKMHGLLEMAVDNNASDLHLTVGVPPVMRIYGKLVFSEMERLT
                     PESTKNLAAEILSQEQLSLLEEEGQVDLSLSLAHIGRFRINVFKQRGSVGLAIRLIPY
                     KIPPFEELNLPPVIRTLAEKESGFILVTGPAGSGKSTTLAAMIDIINSQRSCHIITLE
                     DPIEFLHSHKKSIVNQREIKTDSQSFSAALRAALRQDPDVILVGEMRDLETISIALTA
                     AETGHLVLSTLHTKSAPQSIDRIIDVFPPHQQQQVRIQLANTLVGVVSQRLLPRVDKQ
                     GLIPAVEVMISTPAIKNLIREGKTHQIYSSIQTGSKYGMQTMESSIQDLFERGLIGPD
                     QNMSEIQRLGYMPNVLNP"
     gene            complement(2610..4292)
                     /locus_tag="PK768_06460"
     CDS             complement(2610..4292)
                     /locus_tag="PK768_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQA60649.1"
                     /translation="MKEARRKQLGEMLVEYGLVEPEKLEEALEIQSKTGEKLGKVLKD
                     MGAVSKQELIEILGIQLGIPQVNLQRYLIDPTAVKTIPESLARRLKAVPVKKEEGHLV
                     VAMSNPLDIFAIDDIRLTSGLNVKPVLADEDDILAVINQYYTMKESIEQVLDELAAFG
                     ENNVEVAVENELEASERAPVVKLVNSIIQQGIRTGASDIHIEPGEDIVKIRYRIDGVL
                     HETMKYPKHLHSPVVARIKIISHLDITIKRLPQDGRKKFEFDHTNVDLRISTLPTVHG
                     EKVAIRILDRDKYFMKLENLGFSPENLAEYRKIINYPYGMILVSGPTGSGKTTTLYST
                     LHELDITGKNIVTIEDPVEYMLEGINQVQVQPKIGLTFAEGIRSILRQDPNIIMVGEI
                     RDTETADIAIRAALTGHLVLSTIHTNDSASAVTRLIEMGVEPFLIASSVIGVVAQRLV
                     RKICPECKIEYDADDKELLSLGIGGPQKLNKGKGCSFCNNTGYRGRVAIYEVLKILKD
                     HRRLIINKASADKIKELSIKNGMKTLKENGVGLVLRGVTTVEEILKATTVEI"
BASE COUNT         1069 a         1156 c          978 g         1400 t
ORIGIN      
        1 tccggatact ttccgtgctt cagatagtat ctttccaatg cattttttat aattttctcg
       61 ttagcttcca ttgctttggt ttctgcctca ctcttcaccc caacaacctt gggaattatc
      121 agagtcgcca atactcccag caccgcgatg acgacgatga gctctattaa cgtgaagcct
      181 ttatcaccct tagttctctt atgtaaagcc ctcgcaatct tcattatcat actgtcaccc
      241 cctttcccga ttgcctaacg cagcgtaagt cttttcccat tacccgacaa atctcatcat
      301 ttcaaacatg ggcaggatta tggatatgac tataaagccg attaccactg ccatcactac
      361 gatgatcatg ggctcaagaa tggtggatag cctgtttagc acgtctttga cttccctatc
      421 gtagtaatcg gcaattttgt tgagcatctc ggtcaggctg cccgtttcct ctcctatggc
      481 agtcatctct acgaacagcg gggggaagaa gttgcccgag gcagctgctt cggatattcc
      541 cgctcccttt tgaatgctct ccttgatttt attgcactct atctttaatg tctcactttc
      601 tatcacacgg ttcatggtat ccacggcgtc taagagggga atgccgttta atatcatggt
      661 ggatagtgtt cgggaaaatc tggctagaat caattttgta acaattggtc cgaaaaaagg
      721 aatgttgtaa tatatttttt caagcagcag cctgccttcc ggagttgacc taatcttgat
      781 aaataatagg aaagctaata ccagggccaa gaccagaatc cagaaaaacc ttgacagaaa
      841 atccgatatt acaagtaaaa tcctggtggg gatgggcaaa gcggcgccgt aactttcaaa
      901 catgcccgcg aatatgggaa ggactttcgt cacaattaat ataactacta caattgcggt
      961 gctgctcaat ataatcgggt acgttgtggc agatttgact ttgtttatga tatcattttc
     1021 ccgctcgaag tgttcggcca atctctctag ggcattgttc agtgaaccgc ttatttcccc
     1081 agcctcaacc atatttacaa aaatctccgg aaacacatcc gcattgtttc tgaaagcttg
     1141 acccaaggac aagccgctga ttatatcttc tctggcattt tgggtagctt taacgagaac
     1201 cggacttatc ccctggagct ggaggatttc taaggacttc actatgggta tgccggcacc
     1261 gataactgct gaaaactgcc tgcagtatac ggccaagtct tttggatttg ccttttttgt
     1321 aagattcttt aatatctcta gtatatcccc tgtttcttcc ttttttatgg tgacggggta
     1381 aatccccttg gcccgaagca ttgcggcaac atccgattcg ctttctgctt tgtacatccc
     1441 ctttacactt ttgccctcat tattccttcc cgtatacctg taaattgatg tcaccctatt
     1501 caccccggaa atataaaccg ttttgcaaat accggcactt aaggatttaa cacgttgggc
     1561 atgtagccca atcgctgtat ctcactcata ttctgatcgg gaccaattag tcccctttca
     1621 aacaaatctt gtatggagct ttccatggtc tgcattccgt acttgctccc cgtctgtatc
     1681 gaggagtaga tttgatgggt ttttccctct cttataaggt tttttatggc cggcgtggat
     1741 atcataactt ccaccgcggg gatcaaaccc tgcttgtcca ctcggggaag aagcctctgg
     1801 gacaccactc caaccagggt attggccagt tgaattctca cctgttgctg ctggtggggc
     1861 ggaaatacat cgataatcct gtctatggac tggggggcac ttttggtgtg caaagtggaa
     1921 agcactagat gccccgtctc cgcagcggta agggcaattg aaattgtctc caggtcccgc
     1981 atctctccca ccagtatcac atccgggtcc tgcctcaatg cggctcttaa ggccgccgaa
     2041 aaactctgtg aatccgtttt tatttccctt tgatttacaa tactcttttt gtggctgtgt
     2101 aaaaactcaa ttgggtcttc tagggttatt atatggcagc ttcgctggga gtttatgata
     2161 tctatcatgg ccgccaaggt agtggatttg ccgctaccgg cagggccggt tacaaggata
     2221 aaaccgctct ccttctccgc caacgtccgt atcacgggag gcaggttcag ctcctcaaaa
     2281 ggcggtattt tatagggaat cagccttatg gcaaggccca ctgaaccccg ctgtttaaag
     2341 acatttatcc tgaacctacc gatatgggct agtgacaggg acaggtcaac ctggccttct
     2401 tcctccagca ggcttagttg ttcctggctc aaaatttccg ccgccagatt ctttgtggat
     2461 tccggtgtca agcgttccat ctcagaaaac accagtttcc cgtatatccg cataaccggt
     2521 ggcaccccca ccgtcagatg aaggtccgag gcattgttat cgacggccat ttcaagcagg
     2581 ccatgcattt ttaccatgtc ttgttccccc taaatttcca cagttgtagc ctttagtatc
     2641 tcttccacgg ttgttactcc tcttagcacc aggccaactc cattttcttt taaggttttc
     2701 atgccgtttt taatcgacaa ttccttaatt ttatccgcgg aggccttgtt gatgatgagg
     2761 cggcggtggt ccttcaaaat cttcagcact tcgtaaatgg caactcttcc cctatagccc
     2821 gtattgttgc aaaaggagca ccctttgccc ttgttaagct tttgaggtcc gccgattccc
     2881 aaggataaaa gctccttgtc atcggcatcg tattctattt tgcattcggg acatattttc
     2941 ctaacaagcc tctgtgcaac tacccctatt accgaagatg ctattaggaa gggttccacc
     3001 cccatttcga tcaggcgggt aactgcacta gcagaatcat tcgtatgtat ggtggataaa
     3061 accaggtgac ctgtaagggc cgcacggatg gcgatgtcgg cagtctcggt gtcacgtatc
     3121 tcgccgacca tgattatgtt ggggtcctgc ctcaaaatgc tcctgatccc ctctgcaaag
     3181 gtcagcccga ttttgggctg aacttgaacc tggtttattc cttccagcat gtattctacc
     3241 gggtcttcta tggtcactat gtttttcccg gtaatatcga gttcgtgcaa tgtggaataa
     3301 agagtggttg tcttgccgct tccggtagga cccgatacca gaatcatccc ataggggtag
     3361 ttgatgattt ttctatattc tgcaaggttt tcggggctaa agcccaggtt ttccaatttc
     3421 ataaagtatt tgtccctgtc caggattcgg atcgccactt tttcaccgtg gaccgtgggg
     3481 agtgttgaaa tccgcaagtc cacattcgtg tggtcaaact cgaatttttt ccttccgtcc
     3541 tggggcaggc gctttattgt gatatccagg tgggaaataa tctttatcct ggcaactacg
     3601 ggactgtgca gatgcttggg atatttcatg gtctcatgaa ggacgccgtc tatcctgtac
     3661 cgaatcttta cgatatcctc tcccggttct atgtgtatgt cgctggctcc ggttcgtatc
     3721 ccctgctgaa tgatagagtt taccaacttg accacaggag ccctttccga agcttccagc
     3781 tcattttcca ctgcaacttc tacgttattt tccccaaagg ctgccagctc atcgagaacc
     3841 tgttcaatgg attctttcat cgtatagtat tggttgataa ctgccagtat atcgtcttcg
     3901 tctgccagta ccggcttaac gttgaggccg gatgtaagtc ttatgtcatc aatggcgaaa
     3961 atatccaggg gattgctcat ggccacgacc aggtggcctt cttccttttt cacgggaaca
     4021 gctttcagcc gcctggccag cgattcgggt atagttttta ctgccgtagg atctatcaga
     4081 tacctttgca gattgacctg gggtatgccc agctgaattc ccaggatttc tattaattcc
     4141 tgcttggata ctgcccccat gtcctttagg accttcccca gcttttcccc cgtcttactt
     4201 tgtatttcaa gggcttcctc tagtttttcc ggctcaacca agccgtattc caccaacatc
     4261 tccccgagct gtttccgcct ggcttctttc aaagctttac ctccctaaaa tacacgtata
     4321 tccctatggc taatttacta tatgtcccgg gttttatctg gataaaaagt actaggatgg
     4381 atttatttgt aatatataac aacatgagta tttttgcaac tatttgtgtc aaaaaaactt
     4441 gaaaatcttt gtaaatttat taaaaattat tccgcagctc gtcgggaata gcgccaaagg
     4501 cataaaaaat ccccaggcga acaggcaatc attggcatgg caccgtttag tcctgttgtg
     4561 cccggggtat gcatgaaagt tgcaaagctg attcgaaatg aat
//
LOCUS       DAONUO010000154         4591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13485_length_4591_cov_3.457892, whole genome shotgun sequence.
ACCESSION   DAONUO010000154 DAONUO010000000
VERSION     DAONUO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13485_length_4591_cov_3.457892"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PK768_06465"
     CDS             complement(<1..730)
                     /locus_tag="PK768_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA60650.1"
                     /translation="MKRTEIAIIGAGPAGLSCAVEAARAGAKVTLLDENLKPGGQLFK
                     QIHKFFGSREHQAGTRGFRIGEQLLKETEELGVEVLLNAAVYCIYEDKTLAYIKDGKD
                     YALQADRIVIAAGASENALAFPGCTLPGVMGAGAAQTMINVHRVLPGKKILMVGSGNV
                     GLIVSYQLMQAGADVVALIEAAPQIGGYGVHAAKIRRAGVPVYVSTTIKEVIGEEAVE
                     RVVITSLDEKWQPVPGTERTLEADT"
     gene            complement(727..1044)
                     /locus_tag="PK768_06470"
     CDS             complement(727..1044)
                     /locus_tag="PK768_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQA60651.1"
                     /translation="MRIKHHPILDDDSRKCNASIIVDGKKVPAIEGEPIAAALLAAGI
                     RKIRETSKYHKPRGIFCAIGRCTDCVMIVDGVPNVRTCVTPVRDGMVVRTQKGLGEWG
                     EKP"
     gene            complement(1154..2590)
                     /locus_tag="PK768_06475"
     CDS             complement(1154..2590)
                     /locus_tag="PK768_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQA60652.1"
                     /translation="MSLSAIVWIITVIFCLIFLWISLKVKDDASQSFSHYAIAGGTLP
                     LFLILFTDIATIMGAGNFIGHAAQGYKIGMADIPFVFGEQGSKIIFAIIFAGFAGRFT
                     YNTLSEMMDDLLVRDKVTRAITGLLTACIMIAWVGGQAKGLGDIFAVFTGADPIPIIM
                     LFNVVFIIYTYLGGIYSVVWTDFLQGIIVVIFAFIFYGYALAPVNFSMAVLQQKLAEV
                     GAAELGTLSNVPVGTIMKNFVTGCFGILAAQIYWQRCFAAKDSSTARTGMLISGIAAV
                     VFVSLTAIVGIVTRAVNPNLADPNQAMPWLMLNYVPTWVLAVVYTLILAAAMSSADSN
                     LNAASIILVNDLIRPFAPDKTDQELVKYAQWLTIIVGVFSSLAAIYSSSIIGLFSKAY
                     TMAGGGIVPVLLVGLLWKKTGEPFTMGTRNSRITPWGARLGIIVGSVISLSSLGILWG
                     VAISAVVTIVVSLVTPDVPATEGPSAKV"
     gene            complement(2897..>4591)
                     /locus_tag="PK768_06480"
     CDS             complement(2897..>4591)
                     /locus_tag="PK768_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQA60653.1"
                     /translation="VKKFKVGIIGAGRGGSALLETLIEMDNITIEGIADINSSARGLD
                     AAKKAGIKCFSTVKELLSSCRLDVVFEVTGNKDLARELHSQLPDSTVLVEAPAVNIML
                     SIIRDRKELLEIKEVKEQLSIILNTAHEGIQMVDRNGIIKYVNQGYTRITGIPASQRI
                     GTNVFEVSPDGALVEVLRTGKPCMGKRNKAVGSNAEVISNASPIIINGEMIGAVVVFQ
                     DITEVLRLTEELQRSTSVINGLRDEIIKLSEGRGRFDDIICESSKMKNIINLARKAAR
                     EDSTILITGESGTGKEIFANAIHAESPRAGKPFIKVNCAAIPENLLESELFGYEAGAF
                     TGASKLKLGKFELASGGTIFLDEIGDMSPMLQAKLLRVIQEREIERIGGNKPIKIDVR
                     IISATNRDLLALIGSGAFREDLYFRLNVININIPPLRERREDIPALTRVYIEHFNRKF
                     QKNIKGVTEKAKDMLLSYDWPGNVRELMNILERTILMSQGEWITDDLLRPYFDQVKIK
                     SSSRDAIMPLEEMEKDLIMRALKEFGSSVEGKRKAAEALKISLATLYNKIKKYGIR"
BASE COUNT         1032 a         1210 c         1116 g         1233 t
ORIGIN      
        1 cggtgtcagc ttccagggtc ctttcggtgc caggtactgg ctgccatttt tcatcgaggc
       61 tggttatgac caccctttcc accgcttcct cccctatgac ctcttttatg gtggtagaga
      121 cgtatacggg cacacccgcc cttcgaattt tggcagcgtg gacgccgtag ccgccgattt
      181 ggggggccgc ttcgattaag gctaccacgt ccgctcccgc ctgcatcagc tggtacgata
      241 ctatcaggcc tacgttgccc gagcccacca tcagtatttt cttgccgggc aggaccctgt
      301 ggacgtttat catggtctgg gcggcaccgg cccccatgac cccgggcagg gtgcagccgg
      361 gaaaggccag ggcattttcc gaagctcccg ccgcgatgac tattctgtcc gcttgcaggg
      421 cgtagtcttt gccatcttta atataggcca gagtcttgtc ctcgtatatg cagtatacgg
      481 cggcattcaa gagcacttct actcccagct cttccgtttc ctttaaaagc tgctctccta
      541 ttctaaagcc ccgggtcccg gcctggtgtt ccctggagcc gaagaacttg tgaatctgtt
      601 taaagagctg gccgccaggc tttaagtttt cgtcaagcag ggtgaccttg gcccctgccc
      661 tggccgcttc tacggcgcat gacaggcccg caggtcccgc acctattatg gcaatttcag
      721 tcctcttcat ggcttctcac cccattcacc caaacctttt tgcgtcctta caaccattcc
      781 gtcccttacc ggggtcacgc atgtcctcac attgggcacc ccgtcgacta tcatgacgca
      841 gtcggtgcat cggcctatgg cgcagaatat gcccctgggc ttgtggtact tggaggtttc
      901 ccgaatcttt cgaatccccg ccgcaagcag tgcggcagct atgggttccc cttcgatggc
      961 tggcaccttt ttgccgtcta ctatgatgct tgcattgcat tttcttgaat catcatccag
     1021 gatggggtga tgcttaatac gcaattttgc tacctcctat actatgcgca aatttctttc
     1081 agtacaaaaa attgtgtcaa tatgcttaaa aatgtttagc ccggatttta caccgggcta
     1141 tattctcaag gctttagacc tttgcacttg gtccttcagt tgcaggtaca tccggggtta
     1201 ccagtgaaac gacgatggtc accaccgccg atattgccac accccacagt atgcccaggg
     1261 atgacagcga gatcaccgaa ccgactatta tgccaaggcg ggcgccccag ggggtgatgc
     1321 ggctgtttct ggtacccatg gtgaagggtt ctcccgtctt tttccagaga aggcctacca
     1381 ggagcacagg cactatgcct ccaccggcca tggtgtacgc cttggagaag aggcctatga
     1441 tgctggaaga gtaaatggct gccagggatg agaaaacacc gactatgatt gtaagccact
     1501 gggcgtattt taccagttcc tggtcggttt tatccggtgc aaagggcctt atcaggtcgt
     1561 tgaccaggat gatggaagcg gcgtttaagt tggagtcggc actggacatg gcagcagcca
     1621 gtatcagggt gtataccacg gccagaaccc atgtaggcac gtaattcagc atgagccagg
     1681 gcatggcctg gttgggatct gccaggttgg gattgacggc acgggttacg atacctacga
     1741 tggcggtaag gctgacaaag accactgctg cgataccgct gattagcata ccagttctgg
     1801 cggtggagga gtccttagcg gcaaaacacc tctgccagta gatctgggct gccagtatgc
     1861 cgaaacagcc ggttacgaag tttttcataa tagttcctac ggggacattg ctgagggttc
     1921 cgagttcagc cgccccgact tccgccaact tctgctgcag cacggccatg gagaagttta
     1981 ctggagccaa tgcatatccg tagaatataa aggcgaaaat tactactatg ataccttgga
     2041 gaaagtccgt ccagactacc gagtatatgc ctcctaaata agtatatatg atgaatacca
     2101 cgttgaaaag catgatgatg ggaatgggat ccgctccggt gaacacggcg aatatgtcgc
     2161 cgagaccctt ggcctggcct ccaacccagg cgatcatgat acaggctgta agcaagccgg
     2221 tgatggccct cgtcaccttg tcgcgaacca acaggtcatc catcatctct gaaagggtgt
     2281 tgtaagtaaa tcttcctgca aaacctgcaa atattattgc gaagataatc ttggaaccct
     2341 gttcgccaaa gacaaaagga atgtccgcca tacctatctt atagccttgc gcggcgtggc
     2401 cgatgaagtt gcccgcaccc atgatggtgg cgatgtcggt aaacagaatc aggaagaggg
     2461 gcagggtgcc gccggcaatg gcatagtggg aaaaactttg gctggcgtcg tccttaacct
     2521 ttaatgatat ccataggaaa atcagacaga agataacggt tatgatccat actatggcag
     2581 ataaactcat gaagtaaatt cgcctccttt tttgaatttt ttgcaatctt atacactacc
     2641 cttattattc tgaaataatc acctcctttt tcccacagca aaactttctt actaaaaagt
     2701 atattaacca gaaggtgtaa aaataagcaa tgcaaaaaat atgccaaccg gaaaatgcta
     2761 tggcttgcta tttcgtatga gtaggggtca tcatcgattt ctaaaaaata taaaaaacac
     2821 gaactggaca ggaatatata agcacataag cagtttacag aatttaaaag ttatttctag
     2881 attttagaaa taatcgtcac cgaatgccgt atttttttat tttgttgtac aaagtggcaa
     2941 gagaaatttt taaggcctcg gccgcctttc gtttgccctc cacgctactt ccgaattctt
     3001 ttaaagctct cataatcaag tccttttcca tttcttctaa gggcatgatt gcatccctgc
     3061 tgctggattt gattttaacc tggtcaaagt aaggacgcag caggtcatcg gttatccatt
     3121 ccccctggct catcaggatg gtccgctcca ggatgttcat gagttcacgg acattgcccg
     3181 gccaatcgta tgaaaggagc atgtcctttg ccttttccgt gacccccttt atgttctttt
     3241 gaaattttct gttgaagtgt tcaatataca ccctggtaag ggccggtatg tcctcccgcc
     3301 tttctctgag gggaggtatg ttgatattta ttacattcag cctgaaatac aggtcttccc
     3361 tgaaggcacc cgaaccgatc agcgccagca ggtcccgatt ggtagctgaa atgatcctca
     3421 cgtcaatctt gatgggtttg ttgccgccga tgcgctcgat ttcccgctcc tggataacac
     3481 ggagcagctt tgcctggagc atggggctca tgtcccctat ttcatccagg aaaatggtgc
     3541 cgccgctggc cagttcgaac ttgcccagct ttagcttgct ggcacccgta aaggctccag
     3601 cttcatagcc gaaaagctcg ctctccaaga gattttcagg gatggcagca cagttgacct
     3661 tgataaaggg cttgccggcc ctggggcttt cggcgtggat ggcgttggcg aatatttcct
     3721 tgccggtgcc gctctcaccg gtgatgagga tggtggagtc ctcccttgca gccttcctgg
     3781 ccaggtttat gatgtttttc atcttgctgc tctcgcagat tatgtcgtca aatcttcccc
     3841 gtccttcgct gagcttaata atttcgtccc ttaagccgtt gattacggaa gtggagcgct
     3901 gtagctcctc ggtcaggcgg aggacctcgg ttatgtcctg gaatactacc acggcgccga
     3961 tcatctcgcc gtttattatt atgggagaag cgttggaaat gacttcggca ttcgagccca
     4021 cggccttatt acgcttgccc atacagggct tgcctgtcct caaaacctca accagggcgc
     4081 catctgggga cacttcaaag acattcgtac ctatgcgctg cgaggcgggg atgccagtta
     4141 tcctcgtgta gccttgattt acgtatttga ttattccgtt tctgtccacc atctggatac
     4201 cttcgtgggc agtgtttaga atgatggaca gttgttcctt cacttccttt atttccaaga
     4261 gttccttccg atcccttatg atgctcagca ttatgttgac ggctggtgcc tccaccagga
     4321 cggtgctatc gggcagttgg ctgtgcagtt cccttgccaa atccttgttt cccgtcacct
     4381 caaataccac atccagtctg caggatgaca agagttcctt gacggttgaa aagcatttta
     4441 tgcccgcctt ttttgccgcg tccaaacctc gggcagatga attgatgtcc gcaatccctt
     4501 caattgttat gttgtccatt tcaatcaggg tctccaaaag ggcgctgccg ccccggccgg
     4561 cccctattat ccccactttg aattttttca c
//
LOCUS       DAONUO010000155         4590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13493_length_4590_cov_3.372216, whole genome shotgun sequence.
ACCESSION   DAONUO010000155 DAONUO010000000
VERSION     DAONUO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13493_length_4590_cov_3.372216"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PK768_06485"
     CDS             complement(<1..650)
                     /locus_tag="PK768_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HQA60654.1"
                     /translation="MAQDNTVQSVKRAMMILEELATEKEGLGVTELAKRINLHKSTVH
                     RMLNTLLDIGYVEQNLASEKYRLGMKILFLGGTILERMDIRHEAHDLLKELSEKVNET
                     VHLVVPDGFRAIYIDKMDSNKTIRMHSQIGRIAPLHASAVGKAILAFSEDVFIKELIE
                     RGLEKFTSNTITEPKDLLRHLKMIRERGFSVDDEENEEGIRCVGAPIFNYTGKVIGA"
     gene            887..2125
                     /locus_tag="PK768_06490"
     CDS             887..2125
                     /locus_tag="PK768_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA60655.1"
                     /translation="MTENRIHKNTFIILIALCMGWTLLYADRTALYPLLSVIGDEFNL
                     SSTMMGTITSSYFLVYVSMQIPSGMLADKLGKKRLLILTFIIAGIALLGFGLFAKSYM
                     LLLLFTAIHGFGAGSFYPCAYGIMLNTVDSKQWGIGAAIVNLGMSFGLIVGLAISGPL
                     YLRFQSYSGIFIVLGLITVLAALGFYIILPDVKNESAKSSNSKKAFPLIGILRNSNLL
                     LINTAQFCALYGYWTAVTWGATFFQEERGISMELAGLFVAIVGISAIIPSLLMGRISD
                     KFGRKKIALILFPLGAVTIFLMAYARSNTAIILSLIAYGIFGKSSWDPIAVAWAGSHA
                     TAMDKDALGTAMGVFNFSGMMSAVVAPVVTGFIRDITGSLVAAYYVAALVSVLGGILV
                     MFVSEEPQIFGKDAALESST"
     gene            2247..3635
                     /locus_tag="PK768_06495"
     CDS             2247..3635
                     /locus_tag="PK768_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQA60656.1"
                     /translation="MVNLAQLFSPKDMTVGTPWKRIVEFSIPMLIGNVAQQLYNTVDS
                     IVIGKYVGDNALAAVGSAMPVLHLLLVLFVAVAMGASIMVSQYFGAKDREKLSRSIGV
                     CITLTAIVSTIIMIISPFVIRPMLVMINTPESIIDWCEDYLLILMIGNWGFSYFNILS
                     GILRGLGDSLSALLYLLFATTLNIFLDIWFVAGFNWGVPGVALATAISQMISAVFCAI
                     KLKRMDDIFDLNISMLKPIREYVVQLLKLGLPSGLTQVIFSLAMIVVQSLTNTFGEMI
                     IACNVIVMRVDGFAMMPNFTFGMAMTTYAGQNVGAKKLDRVEEGTRHGIAIAVGVSTV
                     ITIIILIFGRYLMNIFTDTSELVDLSMRMMRILAIGYVAMAITQVLSGVMRGAGDTVT
                     PMWLSLITTIFLRVPTAYGLAYLTRSPQYPNGRPESLFVSLLIAWVLGAAITFFFYKK
                     GDWRNKALLQNA"
     gene            complement(3764..>4590)
                     /gene="hflX"
                     /locus_tag="PK768_06500"
     CDS             complement(3764..>4590)
                     /gene="hflX"
                     /locus_tag="PK768_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQA60657.1"
                     /translation="IELSQEGGGIGTRGPGETKLETDRRHIRRRIHRLKQELKEIQKN
                     RQVIIKSRRYPVISLVGYTNSGKSTLMNALTDAQVVTGDRLFETLDTTTRGLMLPDGR
                     RVLLSDTVGFIRKLPHHLVEAFKSTLEEVKEADMLILVADGSSPVLDEEIATVNSVLR
                     ELEAHRKPTILAINKIDKCGQNAYLKGYDREKIVEISALKGLNLDILLKTICEVLPSS
                     RQFAQLFIPYDEGQALDEIHQHGLLSEIEYKESGIKVKGRIEKAVLKKYEEYAIEQ"
BASE COUNT         1166 a          988 c          994 g         1442 t
ORIGIN      
        1 gctcctataa ctttgccagt ataattgaat atgggggcgc ctacgcagcg tataccttcc
       61 tcgttttctt catcgtctac cgaaaaacct cgctcccgaa tcatttttaa atgccgtagc
      121 aggtccttag gttcggttat tgtatttgaa gtaaattttt caaggccccg ctcaatcagt
      181 tccttgataa atacgtcctc agaaaaggcc aaaatggctt tgccaactgc tgaagcgtga
      241 agcggagcta tgcgacctat ttgcgaatgc atgcgaatcg ttttgttgct gtccattttg
      301 tctatatata tcgccctgaa gccatcggga actaccaggt gtaccgtttc attcactttt
      361 tcggaaagct ctttgagcag gtcatgagct tcgtgccgaa tgtccattcg ttcaagaatg
      421 gttcctccca aaaagagtat tttcattccc aagcggtatt tttccgatgc taggttttgt
      481 tctacatatc ctatgtctag aagcgtattg agcatgcgat ggaccgtgct cttgtgtaag
      541 ttgatcctct ttgcaagttc cgttacgccc aatccttcct tttccgtagc aagctcttca
      601 agtatcatca ttgccctttt taccgattgg acagtattat cctgagccat aatcccaccc
      661 caaaattgag cattaattca agtttgccgt atcgtaatat gaaatgccgt ttcaatcact
      721 gtcttattat attctacata gcttggaaaa ttcccttttt tgttatatat attaaaacat
      781 gaaatcatat ccttatgcaa caaagttgta agtgcaaagc taggatgctt gagcaacttc
      841 tgttataata tatctgtgat tttaaacttg aaatgggggt ctatatgtga ctgaaaacag
      901 gattcacaag aacaccttta taattctcat cgcattgtgc atgggttgga ctttgcttta
      961 cgccgacagg accgcactgt atccgctcct gtccgtcata ggagacgaat ttaatctttc
     1021 aagtaccatg atgggcacta ttacaagcag ttacttcctg gtgtacgtat ctatgcaaat
     1081 tccctcaggc atgctggctg acaaacttgg gaaaaaacgg cttttaatac ttactttcat
     1141 cattgccgga attgctttgt tgggtttcgg tttattcgca aagagctaca tgcttctgct
     1201 cctctttaca gctatacacg gattcggtgc cggatcattt tacccttgtg catacggcat
     1261 aatgctaaat actgttgatt ccaagcagtg gggaataggg gcggcaattg tgaatttagg
     1321 aatgtctttt gggcttatcg ttggactagc catcagcggc cccctgtatt tgcgctttca
     1381 aagttattcg ggaattttta ttgtgctagg acttataact gtacttgctg ctcttggctt
     1441 ttatattata cttcccgacg tcaaaaacga gagcgcaaaa agcagcaatt ctaaaaaagc
     1501 ctttcccctt atcggaatac taagaaacag caacctactg ctgataaata ctgcccagtt
     1561 ttgtgccctt tacgggtact ggactgcagt aacctggggt gcgacctttt tccaggaaga
     1621 gcggggtata agtatggaac ttgcgggcct atttgtagct attgtaggaa ttagtgccat
     1681 aattcccagc cttctcatgg ggcgaatatc cgataagttc ggtcgaaaaa aaatcgcact
     1741 catattattt cccctcggag ctgtaactat ttttctcatg gcctatgccc gctcgaatac
     1801 ggccattatc ctttctctga tagcctacgg catcttcgga aaatcatcct gggacccaat
     1861 agcagttgcc tgggccggca gtcatgctac agctatggat aaagatgcct tgggcacggc
     1921 catgggcgtg tttaactttt ccggtatgat gtcggcagtt gttgccccgg ttgtgaccgg
     1981 atttataagg gatattacgg gttccttggt tgcggcttat tatgtggcgg ccctggtttc
     2041 agtgcttgga ggtattttgg tgatgttcgt cagcgaagaa cctcaaattt tcggaaaaga
     2101 tgctgctctt gaaagtagta cctaacgatg taaaaaaaca tagatttttt ttgggacgtc
     2161 ctttatttat gctatatata catgtataat cgtaattgat gtgcaattaa atataaagct
     2221 ttccgggaag gggttggaat aaggatatgg taaatctcgc gcaattgttc tctccaaaag
     2281 acatgaccgt aggaacgccg tggaaacgaa ttgttgaatt ttctattccc atgttgattg
     2341 gcaatgttgc ccagcagtta tataataccg ttgactcgat cgttatcgga aaatacgtag
     2401 gcgataatgc ccttgcagcc gttggcagcg ccatgccagt attgcacctt ttgctggtcc
     2461 tctttgttgc tgtagctatg ggagcgagta tcatggtctc gcagtacttt ggcgcgaaag
     2521 acagggagaa actatcccga tctatcggtg tgtgtataac ccttacagct atcgtttcaa
     2581 caataataat gataatttct ccctttgtca ttcgtccgat gctcgttatg ataaatactc
     2641 ccgaatcgat aatagattgg tgtgaagact atctattgat cttaatgata ggtaactggg
     2701 gcttttcata ctttaatatt ctttcaggaa tcttgagggg cctcggagac tcgctgtcag
     2761 cgctgttata tctgctcttt gccaccactt taaatatttt tctcgatata tggtttgttg
     2821 ccggctttaa ttggggtgtg cccggggttg cactggctac cgcaatatcc caaatgattt
     2881 cagctgtgtt ttgtgcaatc aagcttaaaa gaatggacga catttttgac cttaatataa
     2941 gcatgctgaa gcctataaga gaatacgtgg ttcaactttt aaaattgggc ttaccctcag
     3001 gcttgaccca ggtcatattc tcattagcaa tgattgtcgt ccaatctctt acaaatacct
     3061 tcggtgaaat gattatcgct tgcaatgtta ttgtcatgag ggtagacggt tttgccatga
     3121 tgcccaattt cacttttggt atggccatga ctacctatgc tgggcaaaat gtaggtgcta
     3181 aaaagcttga cagggtagaa gagggaacac gccatggcat cgctatagcc gtaggagtat
     3241 ccacagtcat aacgataatc attttgattt ttggaagata tctcatgaat atctttactg
     3301 atacgtcaga gctggttgac ttgagcatga gaatgatgcg catcttagca attggatacg
     3361 ttgcaatggc gattacgcag gtgctttcgg gtgtcatgcg cggtgcagga gatacagtca
     3421 cacccatgtg gctttccctg ataacgacta ttttcctccg ggtccctacc gcatatggac
     3481 ttgcctatct tacccgtagt cctcaatacc caaacggaag gcccgaatca ttgtttgttt
     3541 cccttttaat cgcctgggtg ttgggtgcgg ccataacttt cttcttttac aaaaaaggtg
     3601 attggcgaaa caaggcactt ttacagaatg cgtaaaaagg catagaatca ataaaggccg
     3661 atgggtttta aaatacctca tcggctcttt ttgccattgt tcacattaaa gcaatggcta
     3721 atttttaagt ttaaagtaaa aaagaaaagg catataaact gttctattgc tcaatggcat
     3781 attcttcata cttcttaaga acagcttttt caatgcgccc tttaactttt ataccggatt
     3841 ctttatattc gatctcggaa agcaagccgt gctgatggat ttcatctaac gcctgcccct
     3901 catcgtaggg aataaagagc tgggcgaact gacggctcga tggcaaaact tcacatatgg
     3961 tttttaaaag tatatccaaa tttaagcctt tcaaagctga tatttccaca attttttccc
     4021 ggtcatatcc cttaagatat gcattttggc cacatttgtc aattttattt attgctagaa
     4081 tggtgggttt cctgtgagcc tctagctccc taagaactga attaacggtg gctatttctt
     4141 catccaaaac gggacttgag ccatctgcta caagaattag catatcagct tctttcactt
     4201 cttccaatgt ggacttgaag gcttcaacga gatgatgggg aagctttcga ataaagccta
     4261 cggtatcaga aaggagtacc ctgcggccat cgggaagcat gaggccacgt gttgtggtat
     4321 ccagggtttc aaaaagccgg tcacctgtga ctacttgagc gtcagtgaga gcattcataa
     4381 gagtggattt tcccgagttc gtatatccca ccaaactgat gactggatag cggcgggact
     4441 ttataatgac ctgacgattc ttttgtattt ctttaagctc ctgttttaag cgatgtattc
     4501 tcctgcggat atgccgccgg tcggtttcca gcttggtctc ccccgggccc cgggttccta
     4561 tgccgccacc ttcctgggaa agctcaatcc
//
LOCUS       DAONUO010000156         4580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13534_length_4580_cov_3.556685, whole genome shotgun sequence.
ACCESSION   DAONUO010000156 DAONUO010000000
VERSION     DAONUO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13534_length_4580_cov_3.556685"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PK768_06505"
     CDS             complement(<1..398)
                     /locus_tag="PK768_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HQA60658.1"
                     /translation="MKMLAAKRRLGLVFFPAFDWAISPSHPEREERLLYTQDQIFEEG
                     ILDIENIKSYNPLVASIKDVERVHFCVPDVEERVTQSHLISAGGAIKAAQAVMGKEVD
                     KAFALVRPPGHHAQRVVYGDRGFCIINVEA"
     gene            complement(379..2058)
                     /locus_tag="PK768_06510"
     CDS             complement(379..2058)
                     /locus_tag="PK768_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HQA60659.1"
                     /translation="MIVGLDMGGTHVDAVLIHQGEIIEATKRPTDRSNMLQSILDTLE
                     SLLSGQDPSRIERINLSTTISTNAIVENKTDPVGMIIQCGPGLEKDFLRCGRENIFIS
                     GYIDHRGREVSSIDIAEIEETARVFKDRNISALAVVGKFSVRNNAHELKIKEMLENEF
                     FPITLGHSLSGKLNFPRRVFTAYYNSAVYNIFYKFCESIHQAMQRQNIKAPVHVLKAD
                     GGTMSMKAGQQFPVYTILSGPAASVMGGQAFLKTSQDAVFLDIGGTTTDISFLADGVP
                     LLEPLGIKIAGYPTLVRALYSVSIGVGGDSKVTVEGERLVVGPERLGRPKALGGMCPT
                     PTDAMIVLGLMNIGDKRKALSAMKEIGEQLMISPEAAAEKVLDEMADMIKGKVDELLR
                     DINSQPVYTVRELLYGKKIKPRFIQAIGGPAKALSPILAKKFSIPCRYPELFEAANAV
                     GAALARTTSEITMYVDTAQRTLSVPEMGLHETISSAYSFEESRKRALALVREQSRLLG
                     AREDEIEAEIVEESSFNMVRGFYTSGKNIRIRAQTKPGLIYQLWSDEDVGS"
     gene            complement(2072..2983)
                     /gene="murB"
                     /locus_tag="PK768_06515"
     CDS             complement(2072..2983)
                     /gene="murB"
                     /locus_tag="PK768_06515"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQA60660.1"
                     /translation="MELKEIYKIMREILSEDEIKLNEPMREHTSFRIGGPADVMVLPR
                     NSEQVKNIVKLCKKHEIPFFVMGNGTNLLVRDEGIRGVVIKLAQNFNDALIHNNIMKC
                     KAGMPLSMAAKIALDNSLSGLEFAHGIPGTIGGAAVMNAGAYDGEMADVVRKVKVLDL
                     DGNVFELAKSQLEYGYRSCNLQKGDKILLEVELELKPGNYDDIKRKMEDFSQRRKDKQ
                     PLNLPSAGSAFKRPPGNYAGALIEQAGLKGYRIGGAMVSDKHAGFIVNAGNATFEDVM
                     NLILHVQKQVKEKFNVELESEIKIFGD"
     gene            complement(3076..3336)
                     /locus_tag="PK768_06520"
     CDS             complement(3076..3336)
                     /locus_tag="PK768_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60661.1"
                     /translation="MSFKNCAFYDKIMPGKITTVKGISFIPIIGVTIAACGEFALFFY
                     GCISPKGVITVESNGEISLYRISDDTSPSKLLESITLLYGEE"
     gene            complement(3661..4512)
                     /gene="dat"
                     /locus_tag="PK768_06525"
     CDS             complement(3661..4512)
                     /gene="dat"
                     /locus_tag="PK768_06525"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HQA60662.1"
                     /translation="MPTVVYLNGQKVDYEDARISIEDRGFLFGDGLYEVVHVYKGRFF
                     HLDRHLARLEQGAKEIYLNLDFGLNNLANICRKAAKESGYDDASVYIQVTRGAAVRQH
                     AFPDNVKCTWLVIARECKGNPPEFYENGIECITVPDERWGRCNIKTVQLLANCIAKEK
                     AKRAGSFEAIFHKGGAVREGSSSNVFIVKDNKLMTHPADNRILNGITRGVVLEMAERK
                     QIEYVEEVFTLDDLFSADEVFLTGTTTEIMPVVKVDGKLIGDGVPGTMTRLIQEEYQK
                     YIDTVDC"
BASE COUNT         1127 a         1156 c          877 g         1420 t
ORIGIN      
        1 atcgcttcta cgtttattat gcaaaaacct cgatcgccgt atactaccct ctgggcatgg
       61 tggcccggcg gccggacaag ggcaaaggcc ttatcgactt cttttcccat taccgcctgg
      121 gcagctttta ttgctccgcc tgctgagatt aaatgagatt gtgtcaccct ttcctcaaca
      181 tcgggcacgc aaaagtgaac cctttccaca tcttttattg aagcaacgag gggattatat
      241 gactttatat tctctatatc caagatgcct tcttcaaaaa tctggtcctg ggtgtacagg
      301 agcctttcct cccgctcggg atgggaagga ctgatggccc aatcaaaggc tggaaagaat
      361 accaaaccca acctgcgctt agctgccaac atcttcatca ctccacagct gatatattaa
      421 gccgggctta gtttgagccc tgatccttat gttcttacca ctggtataaa aacccctaac
      481 catgttaaag ctgctctcct ctactatctc ggcttctatt tcatcttctc tcgctcccaa
      541 tagtctggat tgttccctta caagggccaa ggctcgcttt ctcgattcct caaagctata
      601 tgccgaactt attgtttcat gtaagcccat ttccggcacc gacagggtcc tttgggctgt
      661 atcaacgtac atggttattt cggaggtggt cctggccagg gcagctccca ccgcgttggc
      721 cgcttcaaat aattctggat atctgcaggg aatggaaaat ttctttgcga gaatgggaga
      781 aagcgccttg gccgggccgc caatagcctg gatgaatctc ggtttaatct ttttgccgta
      841 aaggagttct ctgaccgtat ataccggttg agaatttata tcacggagca gctcatccac
      901 tttgccttta atcatatctg ccatctcgtc taaaactttt tctgccgcag cttctggaga
      961 aatcattagt tgttcgccaa tttctttcat tgctgaaaga gcctttctct tatcgccgat
     1021 attcatcagc cccaagacga tcatggcatc ggtaggtgtc ggacacatgc cgcctaaggc
     1081 cttgggtctg cccagtcttt ccggtccgac caccagcctt tcaccttcga cagttacttt
     1141 actgtctccg cctactccta tcgagacgct gtagagggcc ctgaccagag ttggatatcc
     1201 cgcgattttt attcccagag gttctaatag cggcacaccg tcggctaaaa aggaaatatc
     1261 agtagtagtc cctccaatgt ctaaaaatac tgcatcctgg gaagtcttga ggaaggcctg
     1321 tccacccatt acgctggccg ccggtcccga cagtatggtg tataccggaa actgctgtcc
     1381 tgccttcatg ctcatggtgc cgccgtcggc ttttaagaca tggacggggg ccttgatatt
     1441 ttgcctttgc atggcctggt gaatactttc acaaaacttg taaaaaatgt tatacactgc
     1501 agaattgtag tatgccgtaa agacacggcg cggaaagttc aatttgcccg aaaggctatg
     1561 acctaaagtg atggggaaga actcattttc taacatttct ttgatcttta attcgtgggc
     1621 attgtttcgc acggaaaact ttccgactac tgctagcgcc gaaatattac ggtccttaaa
     1681 aactctcgcc gtttcctcaa tttcggcaat atcaatggag cttacttccc tgcccctgtg
     1741 gtctatatat ccggatataa aaatattttc ccggccgcaa cgcagaaaat ccttctcaag
     1801 gccgggtcca cactgtatta tcattccaac tggatctgtt ttgttttcaa caatagcatt
     1861 ggtagaaata gtggtgctta aatttatacg ctctatccgt gaagggtctt gtccagaaag
     1921 taaggactct aaggtatcca aaatggattg aagcatattg ctgcgatcgg tgggcctttt
     1981 ggtcgcctcg atgatctcac cttgatggat taggacagca tcaacgtgag ttccccccat
     2041 gtccagtcca acgatcaatt ctccccctcc tttaatcgcc gaagatttta atctccgatt
     2101 ccagttccac gttaaatttt tcctttacct gcttttgtac gtgtagtata aggttcatca
     2161 catcctcgaa agtagcattt cctgcgttta ctataaagcc tgcatgtttg tcagaaacca
     2221 ttgctccacc tatcctgtat cccttcaagc cggcttgttc aattagggca ccggcgtagt
     2281 tgccgggggg ccttttaaat gcgctgccgg cactgggaag attgagaggc tgtttgtcct
     2341 tgcggcgctg tgaaaaatct tccatttttc tttttatgtc atcataattt cccggcttta
     2401 actccaactc cacttccagt aatatcttat cacccttttg aagattgcag ctgcggtagc
     2461 cgtactctag ctgagatttg gccagttcaa aaacattgcc gtccaggtcc aatactttga
     2521 cctttctcac tacatctgcc atctcaccgt catatgcccc cgcattcatc actgctgctc
     2581 ccccgattgt gcccggaatg ccgtgagcaa attcaagacc gctaagactg ttgtccaaag
     2641 cgattttggc agccatcgat aggggcattc ctgctttgca tttcataata ttattatgta
     2701 tcagagcatc gttaaagttc tgggctaatt ttattaccac acccctgatg ccttcatctc
     2761 tcaccaggag atttgtcccg ttgcccatta cgaaaaaggg aatttcatgc tttttgcaaa
     2821 gctttactat atttttaacc tgttctgagt ttctcggcaa aaccataaca tcggccggtc
     2881 caccaatgcg aaaggacgtg tgttccctca tgggttcgtt taatttaatc tcatcttctg
     2941 acaatatttc tctcatgatt ttgtatattt cctttaattc catggtatct tcccttcttc
     3001 tgttagcaaa ataccttttg tattccacat attatttcta taaaaaagtt gtaagtcctg
     3061 cattcatata tataattatt cttcaccgta taataatgta atactttcca gcaattttga
     3121 gggacttgta tcatcggaaa tcctgtaaag gcttatttct ccattgcttt caactgttat
     3181 aactccctta ggggatatgc agccgtaaaa aaacagagca aattcaccgc aagctgctat
     3241 agtaacacct atgataggaa tgaagctgat acctttaacg gttgtaattt tccccggcat
     3301 tattttatca taaaaagcgc aatttttaaa actcatgtta gtacactccg ccaagtcgac
     3361 ttctgaagaa ccgtgctgtt gttatttttt gccttctgca tttctttata cacgctgtga
     3421 tcatccatag caaatatgtt cccggcaaaa ctgcattgtg cctgttagcc attttatgga
     3481 accctgaaag ctgattttta tacgccattg tccattttga gaaatcgccg cattagcttg
     3541 cacagccagc ggagtaaaaa atgtacacaa aaataggata ctcggtctgg ggtgcattta
     3601 catctcctcc agaccttata tcctatttat attaaaattt attatgcttt attcaagatt
     3661 ttagcagtct accgtatcaa tatatttttg gtattcttcc tgtataagcc ttgtcatggt
     3721 acccgggaca ccgtcaccta taagcttgcc gtccaccttg accaccggca taatctccgt
     3781 tgtagtccct gtaagaaaga cttcatcggc gctaaacagg tcatccagag taaatacctc
     3841 ttcgacatac tctatttgct tccgctctgc catttccaag acaacacccc gagtaatgcc
     3901 gtttagtatc ctgttatccg ctgggtgagt cattagtttg ttgtccttta cgataaacac
     3961 attgctgctg gagccttccc ttaccgcacc acctttgtga aagatggctt caaaggaacc
     4021 tgctctttta gctttttcct tggcgatgca atttgccaaa agctgaacgg ttttaatatt
     4081 gcagcgcccc caacgctcat ctggtacggt aatacattca ataccatttt cataaaactc
     4141 aggaggattt cccttacatt ctctggcaat gacaagccac gtgcacttta cattgtcggg
     4201 aaatgcatgt tgccttacgg ctgcgcctcg tgttacctga atatataccg aggcatcgtc
     4261 atatccgctc tcttttgcag cttttctaca gatgttcgct aaattgttca ggccaaaatc
     4321 aagatttaag tagatttctt tcgcgccctg ttccaatcgt gccaaatgcc tgtccagatg
     4381 gaaaaatcgt cccttgtaaa catgaaccac ttcatataag ccgtcaccaa atagaaaacc
     4441 cctgtcctcg atgctaattc tggcatcttc ataatccacc ttttggccgt ttaagtatac
     4501 tactgttggc ataaaattcc ccccaattta gtttatattt tagcaaaagc tttatttccg
     4561 ggaactacat ctctgccctt
//
LOCUS       DAONUO010000157         4579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13540_length_4579_cov_3.315650, whole genome shotgun sequence.
ACCESSION   DAONUO010000157 DAONUO010000000
VERSION     DAONUO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13540_length_4579_cov_3.315650"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1401)
                     /locus_tag="PK768_06530"
     CDS             complement(310..1401)
                     /locus_tag="PK768_06530"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HQA60663.1"
                     /translation="MQKIKVGVLFGGQSGEHEVSLMSSVSVMRVMDKTKYDIIPIGIT
                     KEGNWKLYLGDISKIEDGSWQKEAVPALVCPDPAHKCIITMADGKESRYYIDVAFPIL
                     HGPRGEDGTVQSVFELMNIPYVSCGVTSSALCMDKEFSKKILQLSGLPVVDYRVYYKN
                     KLKTGLPEIISDLEESIGYPCFVKPANLGSSVGVSKARNRAELKSAILLAAEYDRKVI
                     VEKSVDAREIECSVLGNDNPRASLPGEIIPSREFYDYAAKYLDGDKSKLILPAPLSNK
                     DVARIQELAVRAFKALDCSGMARVDFLMCKVTGNIYINELNTIPGFTKISMYPKMWEI
                     SGLSYGSLIDELIQLAIERHKQKNELKLA"
     gene            1630..1776
                     /locus_tag="PK768_06535"
     CDS             1630..1776
                     /locus_tag="PK768_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQA60664.1"
                     /translation="MTRDRISMLEWERERLLNQWTRNDGDRIKLLVRIMEIEEQIDEI
                     KKGA"
     regulatory      1922..2085
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2179..3312
                     /locus_tag="PK768_06540"
     CDS             2179..3312
                     /locus_tag="PK768_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HQA60665.1"
                     /translation="MISPMDVRFAVIGGGNGGVAMAGYLAYKGSSVNLYNRTQARIQS
                     LMDNPTIDLTGSVEGSGALNKVTCNMEEAIRDTDIIMITTPATGHYNLARLMAPHLEE
                     GQIIVLNPGRTGGALEVYATLRKSGCQKNVVVAEAQTFIYACRATGPRSAKIFSVKHE
                     VALAAIPAMFTNKVVRLLSGAYPQFTPARNVMETSLNNFGAIFHPAPTLLNSGHIERG
                     ETFEYYLEGITPSIGQMLERLDSERMKVATALGVKAVSARQWLEESYGAKGNSIYEAI
                     QNNPAYKGLTAPKGLDTRYIYEDVPCSLVPISSIASVLGIETPTIDTIIRLANIMTGR
                     NFFEEGRTAKKLGLKDLTANQILEFAETGQYITSIEPDEEVVA"
     gene            3312..>4579
                     /locus_tag="PK768_06545"
     CDS             3312..>4579
                     /locus_tag="PK768_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQA60666.1"
                     /translation="MKKIIAASIGKCVHVAGIMNFLSLAEHEGYITRFIGAAIPVDKL
                     LEAVKEEKPDFVGVSYRLTAEPLKKVLVELKEKIDRLEMPNIKWVFGGTEPTAQVAKE
                     SGIFAFTFDGTEDPDEVIGFLKGKTFEKADDYPQDLISRIWSKYPYPILRHHIGLPSV
                     EETVAAIEKVAESKVLDVISVAPDQNAQEYFFEQDKMDRRLDGAGGVPLRTREDFEAL
                     YAASRRGNYPILRCYSGTKNLIPFAQLLNETIKNAWCAVPLFWYSVLDRRGPRPVEEA
                     IRENQQVMRWHGERNIPVEVNESHHWSLRDAHDTIGVVTAYLAAYNAKKMGVKDYVAQ
                     YMFNVPPSLSPAMDLAKMLAKIELIESLQDENFTVYRQARAGIASLPADLDQAKGQLS
                     ASCYLAMAIKPHIYHVVGYCEAHHAATAEDV"
BASE COUNT         1345 a          976 c         1045 g         1213 t
ORIGIN      
        1 ttggtagggt ccaggccttt ttttcttggt ttttactgta aaagtcaggt tataccaaca
       61 aataaattcg aaaacaacat tcttttgcta ataataagat taatgtgaag gcttgtcccg
      121 accttaatca acaaaaacta tcatacgtgt tcttaataat cgatttagtg ccaattgcaa
      181 ctatattata ataatcatta ttattgatgt caattattaa caataaaaaa gccgaaaact
      241 ggctaagctc atttagacaa aatatgagag aatataagcc aattttcgga caaatgtgaa
      301 acactgtatt taagctaatt tcagctcatt cttctgcttg tgccgctcta tggctaattg
      361 tatcaattca tctatgagag agccatagga aagtcctgaa atctcccaca ttttcggata
      421 catgcttatt tttgtaaagc ccgggattgt atttagctcg tttatataga tattacccgt
      481 tactttgcac atgaggaaat ctactcgcgc cataccgctg cagtctaagg ccttaaaggc
      541 ccgaacagcc aattcttgaa ttctagcaac atctttattg gataggggag cagggagaat
      601 cagcttggat ttgtctccgt ctaggtactt ggcggcatag tcgtaaaatt cacggctggg
      661 gattatttct ccgggcaggg aagccctggg attatcattt cccaagacgg aacactctat
      721 ctcacgggca tccacagatt tttcaacgat gacctttcta tcgtactcgg cagccagcaa
      781 aattgcagat ttgagctccg ctctgtttct tgcctttgaa acaccgacac ttgagccaag
      841 gtttgcaggc ttgacaaaac atggatagcc tatagattct tcaagatcgg agataatttc
      901 aggcaagcct gtttttagtt tatttttata atacacccta taatccacaa caggtaagcc
      961 gctaagctgc aaaatttttt tagaaaactc cttatccatg cagagggcag atgaagtgac
     1021 gccgcagctc acgtagggta tattcatcaa ctcaaaaacg ctctgcaccg tgccgtcttc
     1081 cccccgcggc ccgtgtaaaa taggaaatgc cacgtcaata tagtagcggc tctccttacc
     1141 atctgccatg gttattatac atttatgtgc cgggtcagga catactaagg ccggaacagc
     1201 ctctttttgc cagctgccat cttcaatttt cgatatatcg cctaaataaa gcttccaatt
     1261 accttccttg gttatgccaa ttgggatgat gtcatatttt gttttgtcca ttactctcat
     1321 gaccgatacg gaggacatca gcgatacttc atgctccccc gattgaccgc caaacagtac
     1381 accaactttt attttttgca caagaaccat cctttcaaat ttgcataaaa tgaaatgaaa
     1441 gcagctatca cctgctgaat ctctttttgg caaatatata tctgtagata tatattcatt
     1501 atagtataaa aaaattatac tcacaaataa aaagcagccc taatacttga ctttttgaca
     1561 attttgactt aaaataatat cagaaaataa gattgaattt cattatcagc aactaggggg
     1621 gtagacgaga tgacgagaga caggatatcc atgctcgagt gggaaagaga gaggctctta
     1681 aaccagtgga ctcgaaatga tggcgacaga ataaaacttt tagtccgcat catggaaatc
     1741 gaggaacaaa ttgatgagat aaaaaaaggt gcttaagcat taatatttag tgaccaagtt
     1801 gtgcaaaatt agtccgaaat ggacgaaaaa aaggtcgtga aaggctgtca aaactagccc
     1861 tggtgtagtt gacagcatat tcaatatatg atagaataaa ttgaatcaaa atactgtgct
     1921 tcaatctgga acgcttaatt ctttcggaga agaaagaagc gggggacccg aacaattggg
     1981 gtgaatcccc ttcgaccaag tgaccatagg tcctgatcaa ggggtagggc tacactcttc
     2041 ggcccgaacc cgtcagctaa cctcgcaagc gtggtaagag gaggttgtta gcatgagtgc
     2101 agttttttgt tgcactgaag ctatggatct ccatagcttt aattttgtcc aaaaatacgt
     2161 taaaggaggg gtgttatgat gataagtcct atggatgtgc gctttgcagt cataggtgga
     2221 ggcaacgggg gggtggctat ggcaggctac ctggcctata aaggttcttc ggtaaatctt
     2281 tataatagga cccaggcgag aatccagagc ctgatggata accccacaat tgacctgact
     2341 ggttctgtcg aggggtcagg tgcgctaaat aaagtcacat gcaacatgga ggaggccata
     2401 cgagatacgg atattataat gataactact cctgccactg gccactacaa cttggctcgc
     2461 ttgatggctc ctcacctgga agaaggtcag atcatagttt taaaccccgg ccgcaccggt
     2521 ggagctctgg aggtttatgc aacattgcga aagagcggtt gtcaaaagaa cgtagttgtc
     2581 gctgaggccc aaacttttat ttacgcatgt agggctacgg ggccgagaag tgccaagata
     2641 ttcagcgtaa agcacgaagt ggctctcgct gccattccgg ccatgtttac aaataaagtc
     2701 gtacggcttt tatccggggc ttaccctcaa tttaccccgg ccaggaacgt aatggaaacg
     2761 agcctgaaca attttggtgc gattttccat ccggcaccta ctctgctaaa cagcggtcac
     2821 attgagcgag gagagacttt cgaatactac ctggagggta taactccatc tataggtcag
     2881 atgctggaga gactcgattc tgaaaggatg aaggttgcaa ccgctttggg cgtaaaagca
     2941 gtctcggcga gacagtggtt ggaagagtca tacggtgcca aaggaaattc catttacgag
     3001 gctattcaaa acaatcctgc ttataagggg cttacagctc ccaaaggttt ggatactaga
     3061 tacatttatg aggatgttcc gtgcagcttg gttccaattt cttccattgc atcggtgctc
     3121 ggtattgaaa caccaactat agatacaata attcgacttg caaatatcat gacgggcagg
     3181 aacttctttg aagaaggtag gacagcgaaa aaattaggtc taaaggacct gacggcaaat
     3241 caaattctcg aatttgcaga aaccggccag tatattacct ctattgaacc ggatgaggag
     3301 gtggttgcat aatgaaaaag attattgcag cttcaatagg caaatgcgtt cacgttgcag
     3361 gcataatgaa cttcttgtct cttgctgaac acgaaggtta tattacgcgg tttatagggg
     3421 ccgcgattcc tgtggataaa cttttagaag ctgtcaagga ggaaaaacca gatttcgttg
     3481 gtgtaagcta ccgccttaca gcggaacccc ttaagaaagt cctcgtagag ttaaaagaga
     3541 agattgacag attagaaatg cctaatatca aatgggtttt cggcggtacg gagcctaccg
     3601 cccaagttgc taaagaaagt ggcatatttg catttacctt tgacgggacc gaggatcctg
     3661 atgaggtcat tggctttttg aagggcaaga cttttgagaa agctgatgac tatccccagg
     3721 atttgatatc caggatttgg tcaaaatacc catatccaat tctgcgtcac cacatcggtc
     3781 tgcccagtgt agaggagacc gttgctgcca ttgaaaaagt agctgagtcc aaggtcctgg
     3841 acgtaatatc cgtcgctccc gaccaaaatg cccaggaata ctttttcgag caggataaaa
     3901 tggatcgcag attggacgga gcaggaggag tgccacttcg aacacgagaa gactttgagg
     3961 cactttatgc ggcatcccgc aggggcaact atccgatcct ccgctgttac agcggaacaa
     4021 aaaatctcat tccttttgcc cagcttctaa acgaaacgat taaaaatgcc tggtgtgcgg
     4081 ttccgctatt ctggtacagc gtattggaca ggaggggacc tcgcccggta gaagaagcaa
     4141 tcagggaaaa ccagcaggtt atgaggtggc atggtgagcg aaacattccg gtagaagtca
     4201 atgagtctca ccactggagc ctaagggacg ctcacgatac cataggtgtt gtcacagcat
     4261 accttgctgc ttacaatgcg aagaaaatgg gagtaaaaga ttatgtcgct caatacatgt
     4321 ttaacgttcc cccatctctt tcacctgcaa tggacctcgc caagatgttg gccaaaattg
     4381 agctgataga aagcctgcag gacgaaaact ttaccgttta ccgtcaggca agggcaggaa
     4441 ttgcaagttt accggcagac cttgaccagg ccaaggggca gctttcagca tcctgctatc
     4501 ttgcaatggc catcaagccc catatttatc acgttgtggg ctactgtgag gctcaccatg
     4561 cggcaacagc cgaggatgt
//
LOCUS       DAONUO010000158         4570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13577_length_4570_cov_4.014839, whole genome shotgun sequence.
ACCESSION   DAONUO010000158 DAONUO010000000
VERSION     DAONUO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13577_length_4570_cov_4.014839"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PK768_06550"
     CDS             complement(<1..1047)
                     /locus_tag="PK768_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HQA60667.1"
                     /translation="MNNFFRSISFYLLIIILVLSLVQWYSSQEAAQLRIGYSELIQLI
                     DNGQVSKIKIVENNISGTLKDGRNFVSYVPDITSFMEKIDQPASRGELVVDSEPRPTT
                     PWWTQILSPLLLMVFIIGAWYFFMQQGQGGGRVMSFGKSRAKLHTDDKRRVTFEDVAG
                     VDEAKEELEEIVEFLKYPKKFIEIGARIPKGVLLVGPPGTGKTLLARAVAGEAGVPFF
                     SISGSDFVEMFVGVGAARVRDLFEQAKKNSPCIVFIDEIDAVGRQRGAGLGGGHDERE
                     QTLNQLLVEMDGFGINEGIIILAATNRPDILDPALLRPGRFDRQVVVDIPDVKGREDI
                     LKVHARNKPIAEGVE"
     gene            complement(1156..1719)
                     /gene="hpt"
                     /locus_tag="PK768_06555"
     CDS             complement(1156..1719)
                     /gene="hpt"
                     /locus_tag="PK768_06555"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQA60668.1"
                     /translation="MGGSVLRDHIEQVLITKEQIEEKIKELGEKISNDYRNCDNLLLV
                     GVLKGAVVFMADLIRNIDIPLDIDFMAVSSYGSSTESSGVVRILKDLDDPIEGKDLLV
                     VEDIIDSGLTLSYMCDILKSRKPKSLKICALLDKPSRRKIDLKVDYLGFEIPDYFVVG
                     YGLDYAEKYRNLPDICVLKPEIYQDPQ"
     gene            complement(1819..2337)
                     /locus_tag="PK768_06560"
     CDS             complement(1819..2337)
                     /locus_tag="PK768_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HQA60669.1"
                     /translation="MHKVIITIIIAVIGGVVGHYLKLPAGAMVGAMISVGAANILDVQ
                     PQLPETLNILGQIIIGAALGLSVTKEVLVGLKDYLVPSLLVVILLAVFGVVTGFIVTK
                     LTGVDLYTSLFGSVPGGMHEMVILSQSYDVNHPAVMTIQTVRRVLIVVIYPLLVLLVS
                     KLTGSYMHLLDK"
     gene            complement(2468..3823)
                     /locus_tag="PK768_06565"
     CDS             complement(2468..3823)
                     /locus_tag="PK768_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HQA60670.1"
                     /translation="MRPSAANMLVAFNIIHEEDKEIFDLMKDLAQAHRIINVNAFKES
                     PSMEITHEYAQSIVEKTKNLLGKNINIMNSLGIIVGSKDKSRIDTFHEGAAEVIKTGK
                     EMEITSEQAGKLEGAKPGVNLPIYLNDRIVGVVGITGEPDEVRPFGQLLKISVETMLK
                     QFFLSEQLRMEQNSKELYIHDIINGNFQDKNMFLAKGEVLGYDMSLPRIALTIKINDL
                     TDDRNPKNFHSYQALSAQEKREKILEFIKSMFNNPQHMVSHNGSNHYIVLFALMDLDF
                     ETVKEQINDAVKMLAKEMEKHSIRFCVGVGSYYPDLVGLRKSYKEAVKVLQILDKKSP
                     GKVPRIVFAQDLSLEMMLAVTPDHIINIYKSQVLTKKGAEVFLKDDKLINTLKMYFSS
                     NLNISKTADKLKVTRNTVSARLDKIKKLTGLMPTDFNDAVKLKVLLTALDVKSYTPGQ
                     K"
     gene            complement(3829..>4570)
                     /locus_tag="PK768_06570"
     CDS             complement(3829..>4570)
                     /locus_tag="PK768_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HQA60671.1"
                     /translation="TGYNIVDIEAAKELGITVTNIPTYGTDSVAQMVFAHLLEICHHV
                     AAHSDAVKNGEWTNNVDWCFWKYPLIELAGKTMGIIGYGRIGQAVGRVAQAFGMKVLA
                     NDKYPNKDLESDTLKYVELDELLANSDVISLHCPLFEENKGMINKDSIAKMKDGVIII
                     NTSRGPLIVEDDLAEALNSGKVYAAGLDVVSTEPIKADNPLLKAKNIFLTPHIAWAPK
                     ESRQRLMDIAVNNLKQFLEGSPVNVVNQ"
BASE COUNT         1265 a         1044 c          783 g         1478 t
ORIGIN      
        1 ttcaactcct tcagcaatgg gtttgttcct tgcatggacc tttagtatgt cctctctgcc
       61 cttcacatcg ggtatgtcaa caaccacttg tctatcaaaa cggccgggtc taagtagtgc
      121 tgggtctaaa atatcgggtc tgtttgtagc tgctaaaata atgatacctt catttattcc
      181 gaagccgtcc atctccacca agagttggtt caaggtttgt tccctttcgt cgtggccacc
      241 gccaaggccg gcaccacgct gacggccaac tgcatctatt tcatcgatga acacaatgca
      301 gggcgaattt ttcttggcct gttcaaataa gtctctaacc cttgcagcac ctacacctac
      361 aaacatttct acgaaatctg aaccgctgat gctgaaaaat ggaacacccg cttctcccgc
      421 cacagctctc gccaagagtg tcttgccggt acccggaggc cctacgagca gcacgccttt
      481 tggaattcgt gctccgattt caatgaattt cttgggatat ttcaaaaact ccacaatctc
      541 ttcaagttcc tctttagctt catcgacgcc tgctacgtcc tcaaaagtca cccgccgctt
      601 atcatcggta tggagtttgg ccctactttt gccaaaagac atgacgcggc cgccgccctg
      661 gccttgctgc ataaaaaaat accaggcacc tatgatgaaa accatcagca ataacggtga
      721 gagaatctgc gtccaccatg gagttgtagg ccttggctcc gagtcaacca ccagttcacc
      781 tctgctggca ggttgatcga tcttttccat aaatgatgtt atatccggca cgtagcttac
      841 aaaattccgt ccgtccttta aagtaccgct tatgttgttt tccacaattt taatttttga
      901 aacctgacca ttatctatga gttgaatcaa ttccgaatat cctatcctaa gttgggctgc
      961 ttcctggctg gaataccatt ggactaatga taggaccaga atgattatca atagataaaa
     1021 actaatgctt cgaaaaaagt tgttcaacaa ttcccctcct tccaggatta tttgctccca
     1081 cgatatttta ccacaacgaa tgccttttta caattatacc taaatcatat tgatttagtc
     1141 atcctcaaat gggatttact gcggatcttg atagatttca ggctttaaga cacatatatc
     1201 cggcaaattt ctgtattttt cagcatagtc taatccgtac cctacaacaa aatagtcggg
     1261 gatctcgaag ccaagataat ccacctttaa atcaattttg cgacggctgg gtttgtcaag
     1321 aagggcacat attttaaggc ttttgggttt tcgcgatttt aatatatcac acatatagct
     1381 gagagtaagg cctgagtcaa ttatgtcttc taccaccagc aggtctttcc cttcaattgg
     1441 atcatccaag tcctttaaaa tcctgacaac cccggatgat tcggtagaag aaccgtaact
     1501 tgacaccgcc ataaaatcta tgtctaaagg tatatcgata ttccgaatta aatcggccat
     1561 aaagaccaca gcacctttta acacgcccac aagcaacaaa ttgtcacaat tcctgtaatc
     1621 gttactgatc ttttccccca gctccttaat tttttcctcg atttgttcct tcgttatcag
     1681 cacttgttca atatggtctc tcaacacact tcctcccatc cttatctatt taaaaacgac
     1741 attattttta aaattaatta caacaatttt atcacaatgg tgatagtcta tcaagggcat
     1801 tcgcggagaa gaaaagattt acttatccag caaatgcatg taacttccgg taagtttaga
     1861 tactaatagt accaataagg gatatattac tacgattaac acccgcctaa cagtttgtat
     1921 cgtcattacg gcaggatgat tgacatcata ggattgtgat aggattacca tttcatgcat
     1981 cccgcctgga acgctgccaa ataaggaggt ataaagatca acaccggtaa gtttggtgac
     2041 tataaagcct gtaactactc cgaacactgc taataaaata acaacaagca aagacggaac
     2101 taagtaatct tttagaccta ccaatacttc ttttgttacg ctaagcccca aggcagctcc
     2161 gataattatt tgacctagga tattcaatgt ttcaggcagt tgaggttgta catctaaaat
     2221 attggctgct cccacagaaa tcattgcacc gaccatcgcc cctgcaggaa gttttagata
     2281 atgtccaaca acaccaccta ttactgctat aataatggta attataactt tgtgcatcac
     2341 tattgcccct ttttaatttt tatagtttaa ttacaagtta atgaaaggtc taacctaaaa
     2401 ctggaacaaa aaagcagata tatctacaaa gtgatatatc tgtattttct acaatattaa
     2461 caaatagcta tttttgtccc ggtgtatagc tttttacatc caatgcggtc aaaagtacct
     2521 tgagttttac cgcatcatta aaatcagttg gcatgagacc cgtaagtttc tttattttat
     2581 caagtcttgc acttactgta tttctagtca ctttaagttt atctgcagtc tttgaaatgt
     2641 ttaaattgga tgaaaaatac atcttcaggg tattaatcaa tttgtcatcc tttaaaaaaa
     2701 cttctgcccc ctttttagta agcacctggc ttttatatat attgataata tggtccggtg
     2761 tgactgccag catcatttca aggcttaaat cttgtgcaaa tactatccga ggcactttgc
     2821 ccggtgattt tttgtccaaa atctgcagaa cttttactgc ttctttgtat gacttgcgaa
     2881 gtccgacaag gtccggatag taagagccaa cacctacgca aaatctaata gagtgctttt
     2941 ccatttcttt ggctagcatt ttaaccgcat cgttaatttg ctcctttacc gtttcaaaat
     3001 ctagatccat aagagcaaaa agcacgatgt aatgattgct gccgttatga ctgaccatat
     3061 gctggggatt gttaaacata cttttaataa attcaagaat tttttctcgc ttttcttgcg
     3121 cgcttaatgc ctgatagctg tgaaaatttt tcgggtttct atcatcagtt aaatcattaa
     3181 tctttatggt tagggctatc ctcggcaaag acatgtcata tcccaatact tcgccttttg
     3241 ccaaaaacat atttttatcc tgaaaattgc cgttaatgat atcgtgtata tatagctctt
     3301 tagaattctg ctccatccga agctgctctg acaggaaaaa ctgttttaac atggtttcaa
     3361 cggatatttt gagcaactgc ccaaaaggcc ttacttcatc gggttcaccg gtaatgccaa
     3421 caacgcccac tatcctgtcg tttagatata tggggaggtt taccccgggt tttgcacctt
     3481 ctagtttgcc tgcttgttca gaagttattt ccatttcttt tccggttttt attacttcag
     3541 ccgccccttc atggaatgtg tctatgcgcg acttgtcttt actacccacg attataccca
     3601 aactgttcat aatgttgatg ttcttaccta gcaaattttt tgttttttct actatgcttt
     3661 gagcatactc atgggttatt tccatgctag gactctcctt aaaagcattt acattaatta
     3721 ttctatgtgc ttgagccaaa tccttcatta aatcaaagat ttctttatcc tcctcatgaa
     3781 tgatattaaa agccaccagc atgttagcag ctgatggcct catttactct attggttcac
     3841 tacattaacg ggagaaccct ccaggaactg cttcaagttg tttacagcta tgtccattag
     3901 acgttgtctg gattctttcg gcgcccaggc tatgtggggt gttaggaaaa tattttttgc
     3961 tttgagcagt ggattatcag cctttatcgg ttctgtcgat acaacatcca aacctgcagc
     4021 atagactttt ccgctattta aagcttctgc caaatcgtct tctacaatca acggtcccct
     4081 tgaggtgttt attattatta ctccatcttt catcttggct atagaatctt tgttgatcat
     4141 tcccttgttt tcctcaaaca atgggcaatg taggctaatt acatcggaat tggcaagaag
     4201 ctcatctaat tcaacatatt tcaatgtgtc actttctaaa tctttgttgg gatatttatc
     4261 attagcgagc actttcatac caaatgcttg agcaactcta cccacagctt ggccaattct
     4321 gccatatccg attatcccca ttgttttgcc ggcaagttca atcaaaggat atttccagaa
     4381 acaccagtca acattatttg tccattcacc atttttgaca gcatcgctat gtgcagctac
     4441 atgatggcat atctccaaga gatgtgcaaa taccatttgt gcaactgaat cggtgccata
     4501 tgtaggtatg ttagttaccg taatccctaa ttctttagct gcttcgatgt ctactatatt
     4561 atagccggtg
//
LOCUS       DAONUO010000159         4556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13626_length_4556_cov_2.990669, whole genome shotgun sequence.
ACCESSION   DAONUO010000159 DAONUO010000000
VERSION     DAONUO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13626_length_4556_cov_2.990669"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="PK768_06575"
     CDS             complement(<1..105)
                     /locus_tag="PK768_06575"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60672.1"
                     /translation="MPSQDPKERSRNFNEVALGYTKEDAINEARRCLNC"
     gene            complement(129..971)
                     /locus_tag="PK768_06580"
     CDS             complement(129..971)
                     /locus_tag="PK768_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HQA60673.1"
                     /translation="MFKILDRKDLATSIKQFVIDAPLVAKKAKPGQFVIIRIKEGGER
                     IPLTIADFDAQKGTITIVFQEVGKTTKELGTLKAGDYLTDFVGPLGEPVDFPNHKKVL
                     GIGGGLGIAPLYPKLKYLKEKGVEVTSIIGARSADLLFMEDEIEAVSDRMFVCTDDGS
                     KGRHGFVTAVLKELLEQGEKFDEIIIIGPPILMKIGSEITRPFGIPTMVSLNPIMVDG
                     TGMCGCCRVTVGGETKFACVDGPAFDGHKVDFDELIKRLQTYRDEEQIALEKHEGGSC
                     RCHQ"
     gene            complement(996..1820)
                     /locus_tag="PK768_06585"
     CDS             complement(996..1820)
                     /locus_tag="PK768_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQA60674.1"
                     /translation="MRTLALVGKSGTGKSHKAQLIAKQNGASFIIDDGLLIHGPRVLA
                     GHSAKREKTRLGAIKRAIFHDPIHAEEVKNKLSEMKVDSLLILGTSDSMVSAICKALD
                     LPLPDVTIRIEDVSSEHEINTARMTRKAKGKHVIPVPTLEIKKDFSGFFLDPLRIFRM
                     RKGKPTEILAEKSVVRPTYSYLGKYTINDTTISQIALYAASQVPGIGPGGRVYIENYS
                     TGVVLKLELTVLYGVYVPDVLKQVQEKVVQVIEYMTALNVLKVNIVAKNIYFKESY"
     gene            complement(1974..3383)
                     /locus_tag="PK768_06590"
     CDS             complement(1974..3383)
                     /locus_tag="PK768_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HQA60675.1"
                     /translation="MRTRRFLASILTFLIMVTAFAPVCNAAAAVRVLFNEKYLDFDVP
                     PIIVNDRTLAPFRRIAEELGAEIHWNQESRTVTAYKGDKTVILKIDDKTAYVNETAVE
                     LDTPPIIKNDRTLVPVRFFSEAFGAMVSWDNASRTVLINTGEKASKYIMGYYYSQSYQ
                     DFLENLDKMSSTAVKWYSLDKNGDLTDRDNKRYISVPSGYENVINLSKEKGVEIQMLV
                     FENEAERLSKVLASPESRARLANQILTVVEREGYDGVNLDFEYLKAADKDKYNEFASI
                     LYKELKARGKSLSISLPVKTEKADWWPGYDYVTLGRNCDFVVLMAYDKNPGTPGPQSG
                     IDWVEEVVDYAIARIPAEKVVLGIGYYGYDWGPSGRSSVILKNNGATYLKFADELSRQ
                     YGLQLELDEKSGLYYGKYTDEYGNTHQIWMESDYSVDIKAKMVIRKGLKGVALWRLGY
                     SSPSFWDTLMDNFNPVKFK"
     gene            complement(3572..4147)
                     /locus_tag="PK768_06595"
     CDS             complement(3572..4147)
                     /locus_tag="PK768_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HQA60676.1"
                     /translation="MDLLLEIEKAKELNAFAESFEYDISSKLIHTRDIVVDERVRYQC
                     SYSGCREYGKRLMCPPHTLSVDEFKRVLDRYYLALLLQLKGPIKDNDNWEPETDSWGL
                     KLHEVVYKLEKKAFALGFPFAAGLIGGHCKLCDKCPVERDINASCLHRERARPSMEAM
                     GIDVLSTCKKVGMEIVFVPGEVVWTGLILLA"
     gene            4282..4548
                     /locus_tag="PK768_06600"
     CDS             4282..4548
                     /locus_tag="PK768_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60677.1"
                     /translation="MAEKATAQQFSGRRRFGFLFIFRISPGFFVGFIPRRFAMASSGF
                     APDFDFGNDYDYGYHDDDWCIQRINEQEYMFLRRLGIPEMSVMM"
BASE COUNT         1182 a         1096 c          835 g         1443 t
ORIGIN      
        1 acaatttaaa cagcgcctag cttcattgat ggcgtcttct tttgtatatc ccagtgctac
       61 ttcattaaag ttcctgctgc gttccttggg gtcttgtgaa ggcatgggcg tttttgtttt
      121 gctcctgttt attgatgaca tctgcaagaa cctccttcat gtttttccaa tgctatttgc
      181 tcttcatctc tgtaggtttg gagccgcttt atgagttcat caaaatccac cttgtggcca
      241 tcaaaggcag gcccatctac gcaagcaaac ttggtctcac cgcctacggt tacccggcag
      301 cagccgcaca ttcccgttcc gtctaccatt atgggattaa ggctcaccat ggtgggaatc
      361 ccaaaaggtc ttgttatttc tgaaccgatt ttcattagaa ttggcggtcc tataataatg
      421 atttcatcaa atttttcgcc ttgttccaag agttccttca ggactgccgt gacaaaacca
      481 tgccgacctt ttgacccatc atcggtgcac acaaacatcc tgtcacttac agcctctatt
      541 tcatcttcca tgaataataa atcagcgctt ctagctccga taatactggt aacctccacg
      601 cccttttctt taagatactt gagcttcggg taaagggggg ctattcccaa acctccgcca
      661 attcccagga cctttttgtg gtttggaaaa tctaccggct caccaagggg ccctacgaaa
      721 tccgtcaggt agtcgccggc cttaagtgtt cccagctcct tggttgtttt acctacttcc
      781 tggaaaacta tagttatggt gcctttttgg gcgtcaaaat ctgcgattgt aagcggaatc
      841 ctttcaccgc cttctttaat tctgataata acaaactggc cgggtttagc ctttttagct
      901 actagtggcg catctattac aaattgttta atagatgtcg ctaaatcttt cctgtctaat
      961 atcttgaaca tgttgaacct cctgttttta atattttagt agctttcttt aaaataaatg
     1021 tttttggcaa caatatttac ttttaagaca tttaatgctg tcatgtattc aattacttgg
     1081 accacctttt cctggacctg cttgaggaca tctggtacat aaactccata cagtaccgta
     1141 agttccagtt ttagaaccac accggtcgaa tagttttcaa tatataccct tccgccaggt
     1201 ccgatgcccg gtacctggct tgcagcatac agtgcaatct gacttatggt agtatcatta
     1261 attgtatatt ttcccaaata actgtaggtg ggtcttacaa ctgatttttc agccaggatt
     1321 tccgtgggtt ttccctttct cattcgaaag atccttagcg ggtccaagaa aaatccggaa
     1381 aaatcctttt ttatttcaag ggttggaacg ggaatcacat gcttaccctt ggctttccgc
     1441 gtcatccttg ctgtattaat ttcatgctct gacgaaacat cttctatccg aatagtaaca
     1501 tcaggtaaag gtaaatctaa agctttgcat atagcgctta ccatagaatc ggatgtgccc
     1561 aaaataagaa ggctgtctac cttcatttca gaaagcttgt tcttaacttc ttcagcatgg
     1621 ataggatcat ggaaaatagc tctcttaatc gcgcctaagc gtgttttttc ccgctttgca
     1681 gaatgacctg ccaagactct tggtccatgt atcaaaagac cgtcatctat aataaaactc
     1741 gcgccgtttt gctttgcaat cagctgtgct ttgtggcttt taccggtgcc actctttcct
     1801 acgagggcca gtgtccgcac atctgcacct ccagatgctg tttgttccat tatatcactt
     1861 tttcgaagat ttttgtatac acggcccggc tttcacatta aatattgtaa ttgttcgtta
     1921 aaaatttgtc aattacaatg aaaataacag gcccgtattg ggccttttct agtttattta
     1981 aacttcacag gattgaaatt gtccatcagc gtatcccaaa aggatgggga gctgtagcct
     2041 aatctccaca atgccacgcc ttttaaacct tttcgaatta ccatctttgc ttttatatcc
     2101 accgaatagt cgctttccat ccatatctga tgagtattcc cgtattcgtc cgtatacttt
     2161 ccgtaataca gacctgactt ttcatcaagt tcaagctgta atccgtactg cctggaaagc
     2221 tcgtctgcaa atttcaagta agttgcacca ttgtttttca agataacaga acttctccct
     2281 gaaggccccc aatcatatcc gtaatatcca atgccaagta caactttttc tgccggaatt
     2341 cgagctattg catagtctac aacttcttct acccagtcga ttcccgactg gggaccggga
     2401 gtgccggggt ttttgtcata tgccatgagg actacaaaat cgcagttgcg tcccaatgtc
     2461 acgtaatcat agcccggcca ccaatctgcc ttttcggtct ttaccggcag ggagatgctc
     2521 aaactcttac ccctcgcctt taactctttg taaaggatgc tggcaaattc attgtactta
     2581 tctttatctg ctgccttcaa atactcaaag tccagattta cgccatcata accttccctt
     2641 tccacgactg taagtatttg atttgcgagg cgcgctcttg attcaggact ggccagtacc
     2701 ttagagagtc tttctgcttc attttcaaat acaagcattt gaatttcaac gcccttttcc
     2761 ttggagaggt ttattacatt ttcatagcct gagggcacac ttatatagcg cttattgtcc
     2821 ctgtccgtca gatctccgtt tttatcaagg ctgtaccact tcaccgcagt agatgacatt
     2881 ttgtcaaggt tctctaaaaa atcctgataa gactgggagt agtaatatcc cattatgtat
     2941 tttgaagctt tttcaccggt atttatcagc acggttctcg acgcattatc ccaggagacc
     3001 atagcgccaa aggcttcact gaagaacctg acaggcacca aggtcctgtc atttttaata
     3061 ataggtggcg tgtctagttc tactgctgtt tcatttacgt aagccgtttt atcatctatt
     3121 ttaagtatga cggttttatc acccttataa gccgtaacgg tcctgctttc ttgattccaa
     3181 tgtatttcag cacccagctc ctcagcaatt ctcctaaagg gggccagtgt ccgatcattt
     3241 actataatag gcggcacatc aaaatccagg tacttttcat taaacaatac ccttaccgca
     3301 gcggctgcat tgcaaacagg ggcaaatgct gtgaccatta tcaaaaacgt caagatgctt
     3361 gctaaaaatc ttctagttct catttccctc tccctctaat taataatcta aaatataact
     3421 acattcattt atcacatgca cggaaaaaaa tgggccaaag tcgcccattt cccggtatta
     3481 gtttaggtta aatttattat aagttatccg gcattcctat tcaacaaaaa tcggcagatt
     3541 taataaaaat gtaatatttg agggaaaaat ttcaagcaag gagtattaat ccggtccaca
     3601 caacttcacc aggcacaaat actatctcca tacctacctt tttgcaggtc gataggacat
     3661 ctatgcccat ggcttccatt gaaggcctgg ccctttcccg atgcaggcag ctggcattaa
     3721 tatctctttc aacggggcat ttatcgcaca gtttgcagtg accaccaatc aaacctgcgg
     3781 caaagggaaa gcctaaggcg aaggccttct tttctaattt gtaaacgacc tcgtgcagtt
     3841 tcaatcccca actatcggtt tccggttccc aattgtcatt atcttttatc ggtcctttga
     3901 gctgcaataa aagtgccaga taatagcggt cgagcaccct tttaaattca tcaacgctta
     3961 gtgtatgcgg cgggcacatg agtcgctttc cgtactctct acagcctgag tagctgcatt
     4021 ggtacctcac tctctcatct acaactatat ccctcgtgtg aattagtttg gaactgatat
     4081 catattcaaa agattcagca aatgcgttaa gctctttggc cttttcaatc tcgagcaata
     4141 aatccatcga catagctcct ttcataaaaa tttccaatta cttaaatatc attatacctt
     4201 cctcccgcct catagggcaa tagcacaatt tcaaataggg cctcatacaa tatattagct
     4261 aaaaggaaag gagatggttt agtggccgaa aaagcaactg cacaacagtt ttccggaagg
     4321 agaagatttg gcttcctgtt catttttcgc atatcacccg gattttttgt aggttttatc
     4381 cccagaagat ttgccatggc ctcttccggc tttgctcccg actttgattt cggcaacgac
     4441 tatgactatg gctaccacga tgacgattgg tgcattcagc gcataaatga acaggaatac
     4501 atgtttttaa gaagactggg cattcccgaa atgagtgtca tgatgtaaaa tcgagt
//
LOCUS       DAONUO010000160         4522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13726_length_4522_cov_4.486680, whole genome shotgun sequence.
ACCESSION   DAONUO010000160 DAONUO010000000
VERSION     DAONUO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13726_length_4522_cov_4.486680"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..803)
                     /locus_tag="PK768_06605"
     CDS             complement(213..803)
                     /locus_tag="PK768_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HQA60678.1"
                     /translation="MMLSENSFTPRSDEELQKLTVGELKPHNAPITIVEYDPSWPELF
                     EREANRIRSVLGSKALQIEHVGSTSVPGLCAKPIIDILLVVKNSADEPSYVPALEAAG
                     YRLQIREPEWFEHRMFKGPNTDINLHVFSEGASEIDRMLRFRDWLRSNESDREKYAQA
                     KRSLAKNKWRHIQHYADAKTSIVREIMDRANDSNAK"
     gene            complement(859..1746)
                     /locus_tag="PK768_06610"
     CDS             complement(859..1746)
                     /locus_tag="PK768_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQA60679.1"
                     /translation="MNLLKQFPQSIQQFLIESYGKPLFIHKLNGIKAEGGCYRVGFSN
                     HSIIVKQMTKPQEYLFYNKCSSLLKEFNKHIPSLYWSYKDENKYWIVVEDIPYALPKE
                     RWRKADEQVLRALFLFHTESWGKSLPIENFYIPKWDHRLTESVLALYSDKTGNQLQLL
                     LIKTQEDSQQLFKPYCWISADTNPTNWGVRKDGSVVLFDWERISCGSPAIDLSIIMPG
                     LGTPDNSLELSIAKKYLKMWSSAGLDFPISERKLLWQIKLAKIWSVVEFLDNTADTLV
                     ADAFQEILSGLAEKLYKLY"
     gene            complement(1868..2617)
                     /locus_tag="PK768_06615"
     CDS             complement(1868..2617)
                     /locus_tag="PK768_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQA60680.1"
                     /translation="MDLLELIIEYHKDNERQGPGSEEATLKALSYIPDLNEKTKILDI
                     GCGTGGQTITLAKNTTAHITAIDMLPQFLEKLIQKVKENNLIDRITVKEMLMDSLTFE
                     EKSFDVIWSEGAIYNIGFEKGLLLWRKYLKDDGYIAVSEISWLTDTRPDEIEQYWVNA
                     YSEIDTIKNKLSVIEKCGYTNVAHFALDDKCWIDNYYQPLIDNSEAFLKKYNYADEVK
                     DFLEQGKIEADMYNRFKDYYSYVFYIAKKKQ"
     gene            complement(2855..3067)
                     /locus_tag="PK768_06620"
     CDS             complement(2855..3067)
                     /locus_tag="PK768_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60681.1"
                     /translation="MLKDYQNLAIKKVRENAKEKIEEHRVYLERIINQFGVDVEKLIN
                     QLLQSPITINFHPDRLSNDSEISKKD"
     gene            complement(3222..3626)
                     /locus_tag="PK768_06625"
     CDS             complement(3222..3626)
                     /locus_tag="PK768_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60682.1"
                     /translation="MDYYGTQGSSGKINLRTMKQSAEDLYEWARYHYPDSEMIIIGHS
                     YRAGIATYLASVKECYSLIIAAGYRDISDLYNKIIPVSWGPLKVFISNNIRTTEYAKN
                     VECPVYIIGSTGDKVLSASLQENFYAILKIRK"
     gene            complement(3978..>4522)
                     /locus_tag="PK768_06630"
     CDS             complement(3978..>4522)
                     /locus_tag="PK768_06630"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQA60683.1"
                     /translation="RGAKYVLGLDLCEPMIEAARKLQSECDAYRVQDVQDLDFIENET
                     FDVVVSYLNQCDLPDFEANTKEVFRVLKKGGRFIVANLHPMRSATGKWHRNADGEKLH
                     VILDNYFDEGERYWEMVGIRITSFHRSLSTYINNFIKTGFVIKGIVEPTVTVDQLNEY
                     PDLDDELRVPNFIVYVLQKP"
BASE COUNT         1316 a          978 c          689 g         1539 t
ORIGIN      
        1 agggcataga ctcgcctaat ctggtcgttc caggcgcgct actgcctcaa tatggctcga
       61 gttgatggaa ttgatgtctg cgggccattt tttaaagcat ctgtacgtag gaacatgtca
      121 atggggtttt ggcattatcc ccgaaaacgg acgttcaggg aaacatatca aatttcacat
      181 aataagcaag taaaaccacc tttctttcat tattatttag catttgaatc attcgcacgg
      241 tccataatct cccgcactat tgatgttttg gcatctgcgt agtgctgaat atgccgccac
      301 ttattctttg ccaagcttct cttggcttgt gcatattttt cacgatcact ttcattagaa
      361 cgcaaccaat cacggaaacg taacattctg tctatttcag acgctccctc actgaacaca
      421 tgcaagttaa tgtcagtatt tggtccttta aacatacgat gctcgaacca ttctggttct
      481 ctaatttgta gtctatagcc ggctgcttcc aatgccggga cataggatgg ctcgtcggca
      541 gaattcttta caactaacag tatatcaatt attggtttgg cacaaagccc cggcaccgaa
      601 gtggagccaa catgttcaat ttgcaaagct ttgctgccga gtaccgaacg aatcctgtta
      661 gcttcccgtt caaacaattc aggccagctc gggtcatatt caacaatagt gattggtgca
      721 ttgtgtggtt tcagttcacc cactgtaagc ttctgaagtt cttcatcact cctaggtgta
      781 aagctatttt cagatagcat catatcacac tcctttacta tttacgtgat ctaacttttt
      841 caaaaatgca gaatctaatc agtataactt gtacagtttt tctgctaatc ctgataaaat
      901 ttcctgaaaa gcatctgcaa ccaatgtgtc agcagtatta tctaagaatt caactacact
      961 ccaaatcttg gcaagtttta tctgccaaag caatttcctc tctgaaatcg ggaaatcaag
     1021 ccctgcactc gaccacattt ttaaatactt cttggcaatt gataactcta aagaattatc
     1081 cggtgtgcct aacccgggca taattataga aaggtctata gccggtgaac cgcaactaat
     1141 tctttcccaa tcaaaaagga ctaccgagcc atcttttctg actccccaat tagttggatt
     1201 cgtatcagcg cttatccagc agtaaggttt aaataattgc tgcgaatctt cttgtgtttt
     1261 aatcaagaga agttgtaatt gattacctgt tttatcagag tataatgcca acacactctc
     1321 tgtcaatcgg tgatcccact ttggtatgta gaaattttca ataggaaggc tttttcccca
     1381 actttccgta tgaaacagaa ataatgctct cagtacctgt tcatcagcct tccgccacct
     1441 ttcttttggg agagcatagg gtatatcttc taccacaatc caatatttat tttcgtcttt
     1501 atatgaccag tataaagatg ggatatgctt attaaattcc tttaaaagag aggaacattt
     1561 attataaaat aaatattcct gaggttttgt catctgctta actataattg aatggttgga
     1621 aaaaccaacc cgataacatc caccttcagc tttaatacca tttaacttat gtataaaaag
     1681 aggtttcccg tatgattcta ttaaaaactg ttgtatgctt tggggaaatt gttttagtag
     1741 attcatccgt tatcataatc ctttctcttt caaaacctta caacctttcg taaaatttcc
     1801 tcgggttcgt caatatatct tttactatct catacaaaac attgaatcag tctaaaacac
     1861 ctattcacta ttgctttttc tttgcgatat aaaatacata gctgtaataa tccttaaatc
     1921 tgttgtacat atccgcttct atcttgcctt gttcgagaaa atcttttacc tcatctgcat
     1981 aattatattt tttaaggaac gcttcggaat tatctataag cggctgataa taattatcga
     2041 tccagcactt atcatccagt gcaaaatggg cgacgttagt atatccgcat ttttcaataa
     2101 ctgacagctt attttttatt gtatcaattt ccgaataggc attaacccag tactgctcga
     2161 tttcatccgg tcttgtatcg gtcagccagg agatttcaga aacagcgata taaccgtcat
     2221 ccttcaaata ttttctccac agcaacagac ccttttcaaa accgatgttg tagattgccc
     2281 cctccgacca aattacatca aaagattttt cctcaaaggt taagctatcc attaacattt
     2341 ctttgactgt tatacgatca atgaggttat tctccttgac tttttgtatc agcttttcta
     2401 aaaattgcgg cagcatatcg atagccgtga tatgtgcagt tgtatttttt gcaagggtta
     2461 ttgtctgtcc gccggttcca cagccgatat cgagtatttt agttttttca ttcaaatcag
     2521 gaatatagct tagtgctttt aatgttgctt cttcactgcc cggtccctga cgttcgttat
     2581 ctttgtgata ttctatgatt aattctaaca aatccacttt tattctcctc tcgtattata
     2641 aactaaataa actttatgcc tttagcaata gcagaacaat aaataatgat gtcagccaaa
     2701 atactcaatt gtatgccata gttgttttat gtattggaac acctctgatt tacttccaat
     2761 gtcactccat ttttccggat taaggtcact gtctcgagat gctttcccca aaagggcaac
     2821 attcataact cctttgttat gtcccaaaac agtatcaatc ttttttgcta atttcggaat
     2881 catttgataa tctatcaggg tgaaagttta tggtaatagg actttgtaat agttggttaa
     2941 tcagtttttc aacatctaca ccaaattgat taataattcg ttccaaataa actcgatgtt
     3001 cttcaatttt ttctttagca ttttccctta cctttttaat agcaagattt tgataatctt
     3061 tcagcattag tgctccccct accactaaca taatttacat ctgttctaat caactgtttt
     3121 ctataaccag ctgtcttttc attagtcaat tgcttgtttt ataatattaa ttacattatc
     3181 tgaacaaaaa taattatcgt gagaaatatc gtcgaatatt tctattttcg gatttttaaa
     3241 atagcataaa agttttcttg caacgaagca cttaaaactt tatcgccagt agaaccaata
     3301 atgtaaaccg ggcattccac attcttagca tattctgtag tgcgtatatt atttgaaata
     3361 aacaccttta atggtcccca ggatacagga atgattttat tatataaatc cgaaatatcc
     3421 ctgtaccctg cagcgataat caaagagtaa cattccttga cgcttgccag atatgttgca
     3481 attcctgctc tgtaactatg tccaattatt atcatttcac tatcgggata gtgatacctt
     3541 gcccattcat ataaatcttc cgcggattgt ttcatcgttc ttaaatttat tttgccactg
     3601 ctgccttgag ttccataata atccactgaa ataaataggt aatcaaattt agaggaatac
     3661 tttccaacag aattgtatgc aatataatcg gaaccaccaa aaaataatat aaccttattg
     3721 ttatttttag tatcaaggtt attaccatag cctgttaatg catctttaaa ctgaataaat
     3781 tacggagtac aagaaatatc tgtcatcttt ttctccattt cgtatttccc tcaaatttct
     3841 tttatctttg cgcattatgc ccactcaatc ctcgaccaat ttttcaagct taatatccat
     3901 aagtataaag cctgtttgtt atctcattaa aagcattaat tagtttgctt ccaaaattct
     3961 tcgtcacccc ccatatatta gggcttttgc agaacataaa caatgaaatt tggcacccgg
     4021 agttcatcat caagatccgg atattcattt aactgatcca cagtcacagt tggctcgaca
     4081 attcctttta taacaaaacc agttttaatg aaattattta tgtaagtgga tagcgaacga
     4141 tggaaactgg tgattctgat ccccaccatt tcccaatacc gttctccttc atcaaagtaa
     4201 ttgtccaata tgacatgcag cttttctcca tccgcatttc gatgccactt cccagtcgca
     4261 gacctcattg ggtgcaaatt agcaactatg aatctaccac ccttcttcaa tacccgaaac
     4321 acttctttgg tattcgcctc aaaatctggc aggtcgcatt gatttagata tgaaaccaca
     4381 acatcaaaag tttcattttc aataaagtca aggtcttgca catcctgtac acggtaggca
     4441 tcacactcag actgcaattt ccgtgcggcc tcaatcattg gctcgcatag atccaatccc
     4501 agcacatact ttgctccacg tt
//
LOCUS       DAONUO010000161         4519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13737_length_4519_cov_4.406138, whole genome shotgun sequence.
ACCESSION   DAONUO010000161 DAONUO010000000
VERSION     DAONUO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13737_length_4519_cov_4.406138"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="pdxS"
                     /locus_tag="PK768_06635"
     CDS             complement(<1..795)
                     /gene="pdxS"
                     /locus_tag="PK768_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HQA60684.1"
                     /translation="MSNQINERLKGGVIMDVTTPEQSRIAEEAGAVAVMALERVPADI
                     RKQGGVVRMSDPKIIKAIKESVSVPVMAKCRIGHFVEAQILEALGVDYIDESEVLTPA
                     DESYHIDKTQFKTPFVCGARNLGEALRRIGEGASMIRTKGEAGTGNVVEAVKHIRTVM
                     GQIRNLSNMPEEELMTAAKEMGAPFELVKQVAKTGKLPVLNFAAGGIATPADAALLMQ
                     LGCDGVFVGSGIFKSEKPEKMARAIVQAVRNYDDPDALARISEGLGE"
     gene            946..2436
                     /locus_tag="PK768_06640"
     CDS             946..2436
                     /locus_tag="PK768_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQA60685.1"
                     /translation="MDKYVSIHLDPSSAKPLYLQICDGVADLIEKGKLPYGEKLPSIR
                     QMASFFNVNAVTVVNAYKQLEAQGYVEARVGSGTYVSFAANVKDFDEFTTADDAAPLP
                     REARSVLESAALPRRFDFAGASISPEYFPVKDFKDVVNEVLDRDGGYALEYQESSGYH
                     PLRESIADYMKTQHDLLVPFEEVQIVSGAQQGIDILAKAFLNYGDVVYVEAPTYPGAV
                     NAFRSRGAHIVEIEMEGDGIDLEDLEKKLAKNKPKLLYTMPVFQNPTGNSYSMKKKQE
                     LIALAERHGFYIIEDDHISDLYYRQKPTMLKAIDDADRVFYIKSFSKLFLPGLRLAFL
                     WVPKAYSDKIALAKYSSDISSSGLFQRALDLFLRKGLWPKHLRRLRKVFYEKWQKTHD
                     ALAEQMPGELKYNRPEGGHFFWLQLPRGFYSMNLYQVAMKKGVFIMPGNLFYMSQRPS
                     EGFRLSFAQISARDIDEGIQKLSESIKLFLQNNYTIIQGPNDRPLL"
     gene            complement(2454..3122)
                     /locus_tag="PK768_06645"
     CDS             complement(2454..3122)
                     /locus_tag="PK768_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQA60686.1"
                     /translation="MISDFEETGYSLRSKIYHRLKNAILDGVYKPGESLIEMKLAKEL
                     GVSRTPIREAIRQLELEGLVSSIPNKGVVVEGVTMQDVEDIYDIRKMIEGLAARWAAE
                     KITDEQLKELKDILDLMEFFTDKENVEKFSELDSKFHEIIFKASNSRPLESVLSNFHH
                     FIQRARLVSVKTKGRIACSLEEHRRIYEALAAHDPDAAEKAMTEHVGYAKMNLSPYFE
                     KQKT"
     gene            3588..4421
                     /locus_tag="PK768_06650"
     CDS             3588..4421
                     /locus_tag="PK768_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQA60687.1"
                     /translation="MIHIVTDSSCDLPDEYLKEHDISFVPLTVRFEDDKEYRERIDIG
                     PEEFYNKMTASRTLPKTAQPSPADFLKVFEEVAAPGDEILCITISSKLSGTYQSATLA
                     KKMTSIPVTVFDSLAASPGHALQIMRAVELRKLGYGIDKIVAELTYYRDNEMKIFIYF
                     KTLENIVKGGRITKFQGTLAKVMNIKLICEGIRGEVVPLEKVRGDKRALERLISIIGQ
                     QRDDFSDTILCMTHTGNLEDAEYIKAELIKRYNPKEILIYPTGSTVATYAGPGGIVIT
                     F"
BASE COUNT         1206 a         1034 c          978 g         1301 t
ORIGIN      
        1 ctcgccaagg ccttcggata ttcttgccag tgcatcggga tcatcgtaat tcctgacggc
       61 ctgcacaatg gcccttgcca ttttctcggg cttttctgac ttaaatatac ccgagccaac
      121 aaatactcca tcacagccca gctgcatcaa aagggcagca tcggcaggtg ttgcaatgcc
      181 tcctgcggca aagttcagta caggaagttt gccggttttg gctacctgtt ttaccagttc
      241 aaagggggcc cccatttcct tggctgcggt catgagttct tcttccggca tgttggaaag
      301 atttctgatt tggcccatga ccgtgcggat gtgcttcacg gcttctacca cgttgcctgt
      361 gcctgcctct cctttcgtgc gtatcatgga tgctccctct cctatgcggc ggagggcctc
      421 tcccaggttc ctggcaccgc acacaaaggg cgtcttaaat tgggttttgt caatgtgata
      481 ggattcatca gcaggtgtca ggacctcgct ctcatctatg tagtcaacac ctagggcttc
      541 taaaatctgc gcttcaacga aatggccgat tctgcatttg gccatcacag gaacagatac
      601 cgattctttt attgccttga tgattttagg gtccgacatg cggacaactc cgccctgctt
      661 cctgatatct gcagggaccc gctccagggc catcacggct acagccccgg cctcttcggc
      721 aattctggac tgttcgggcg tggtgacatc cattattact ccgcccttca gcctttcatt
      781 gatttgattt gacataaagc acaacctcct tgtttgctcc ctttcggcaa tgtggtataa
      841 taaatgtacc ggtgtataat cctggaagac tatacgtaca ctattatgtc attctcgcaa
      901 gtgtttgtca atacacttta taaatgttct gtatcggagg ttaatatgga taaatatgta
      961 tcgatacatt tagacccctc ttcagcgaaa cccctttatc tgcaaatatg cgatggtgtt
     1021 gccgatctaa ttgaaaaagg caagctccct tacggagaaa agcttccttc catccgccaa
     1081 atggcctcgt tttttaatgt caatgccgtc acagttgtca atgcgtataa gcagctggaa
     1141 gcccaaggat atgttgaagc tcgcgtcggc agcggcactt atgtgtcttt tgccgcaaat
     1201 gtgaaagatt tcgatgaatt taccaccgca gatgatgcag ctcccctgcc tcgggaagca
     1261 aggtctgtat tagagagtgc agctttgcct agaaggtttg actttgcagg ggcttccatt
     1321 tcccctgaat attttcccgt caaagatttt aaggatgtgg tcaatgaggt gttggacagg
     1381 gatgggggct atgctttaga gtaccaggag agcagcgggt atcatcccct tagggagtcg
     1441 attgcggatt atatgaaaac gcagcatgat ctattagtac cttttgaaga ggttcagatc
     1501 gtttccgggg cacagcaggg catagacatt ttagccaaag cctttttaaa ttacggcgat
     1561 gtggtctacg tcgaagcgcc aacatatccc ggggccgtca atgcctttag atcccggggt
     1621 gcccacatag tagaaattga aatggaagga gatggaatag atcttgaaga tttagaaaaa
     1681 aagctggcca agaataaacc aaagctcctt tacaccatgc cggtctttca aaatccaacc
     1741 ggcaactcgt acagcatgaa aaaaaagcaa gagctgatag ctcttgctga aaggcacggt
     1801 ttttatatta tcgaagatga ccacataagt gacctttact accgtcaaaa gccaactatg
     1861 ctaaaggcca tcgatgatgc cgacagggtg ttttatataa agagtttttc caagctgttc
     1921 ttaccgggcc tgcgcctggc tttcctgtgg gtgccgaaag cgtattcgga taaaatcgct
     1981 ctggccaagt actcatcgga tatatcttct tcaggacttt ttcagcgtgc attggacctc
     2041 tttttaagaa agggcctttg gccaaaacat ctaaggcgcc tccgaaaagt attttatgaa
     2101 aagtggcaga aaacccacga tgcccttgca gagcaaatgc ctggagagct taaatataac
     2161 cgaccggaag gcggccactt cttttggctg cagctaccca ggggtttcta ctccatgaac
     2221 ctgtaccagg tagctatgaa aaaaggtgta ttcataatgc ccggaaacct gttttacatg
     2281 agtcaaagac cctcggaagg ctttagactt agttttgcgc agatctcagc ccgtgatata
     2341 gatgagggaa tccagaagct ttccgaaagc ataaaactct ttttgcaaaa taactacaca
     2401 atcattcaag ggccaaatga ccgccccctg ctctgagaca agaattacaa aaactacgtt
     2461 ttttgttttt caaagtaagg agagagattc atcttggcgt atcctacgtg ttccgtcatg
     2521 gctttttccg ccgcatcggg gtcatgggct gccagggctt catatatgcg gcggtgttct
     2581 tccagggaac aggcaatcct gcccttagtc ttcaccgaaa ccagccttgc cctttggata
     2641 aaatggtgaa aatttgaaag caccgattct aagggcctgc tgttactggc tttaaatata
     2701 atttcgtgga acttagagtc cagttccgaa aatttttcta cattttcctt atcggtaaaa
     2761 aactccatta ggtctaaaat gtcctttagc tccttaagct gttcatcagt aatcttttcc
     2821 gcagcccacc ttgccgcaag gccttctatc atcttacgaa tgtcatagat atcttccaca
     2881 tcctgcatgg ttacaccttc gaccacaaca cccttgttgg gaatgcttga taccagtcct
     2941 tcaagttcca gctgccttat ggcttccctt atgggggtgc ggctgactcc gagctccttg
     3001 gccagtttca tctctattaa actctctccg ggcttgtaga ctccatctag tatggcattt
     3061 tttaaacgat gatatatctt actcctaagg gaatagccgg tctcttcaaa atcggatatc
     3121 atatgataac ctccctgata attgagaaaa atcctatatt tattctacta cacatttgtt
     3181 gttttttaaa gggattcatg tactaatata tacatcctct aaaatatgaa gcatcttttg
     3241 tcttatttca cagggaggaa aagtatttaa ttgttttacg caccttgcaa caaagctgtt
     3301 gcagtacttt gccgcttcct ttgcagcagc gccttttaat tttgaaacac atgaatgttt
     3361 ttgacttgtg tagtcatctt ttaaagataa aaaaagctct tggggatttt ttgttttgtt
     3421 cattttgaag agcaaaatca ctttattgat atctggcgca gtacgttcat caggttagca
     3481 tcatagtata gaaaatccca ttcaatttag ttataatata atttgcggta gcaatgagac
     3541 ataaatttca gcaatgtcaa attcacatga ttcgaggtgg aagcacattg atacatatcg
     3601 ttaccgatag ttcctgtgac ttgccggatg aatatctcaa agagcacgat atttctttcg
     3661 tccccttgac cgtcaggttt gaagatgaca aggagtatag ggaaaggatt gacatcgggc
     3721 ctgaagagtt ctacaacaaa atgactgctt caaggactct tcccaaaact gcacagcctt
     3781 cccctgctga ttttctcaag gtttttgaag aagtggcagc tcccggtgat gagatcttgt
     3841 gcataactat ttcatcgaaa ctcagcggga cctatcaatc tgccactttg gccaaaaaaa
     3901 tgacttccat accagttaca gtcttcgatt cccttgccgc ttcaccgggt cacgccctgc
     3961 agattatgag agcggttgaa ctaaggaagc tgggatatgg tattgataag attgttgctg
     4021 aattaactta ttacagggac aatgaaatga aaatattcat atattttaag accctggaga
     4081 acatagtgaa gggcggccgc attaccaagt ttcaaggcac gctggccaag gttatgaata
     4141 ttaagcttat ctgtgaagga ataaggggcg aagttgtgcc cttagagaag gttcgagggg
     4201 ataaaagggc cttggaacgc ctaatatcaa taattggcca gcaaagagat gacttttccg
     4261 atacaatcct ttgtatgacc catacgggaa atctcgagga cgccgagtat attaaggccg
     4321 aattgataaa aagatataac cccaaagaaa tactaatcta tcccaccggt tccaccgttg
     4381 ccacttatgc agggcccggc ggcatagtca taacttttta gaaggtaaaa ggcctgtgga
     4441 ttttcatttc cacaggccct ttatatttca aagtcaaatc tttactttga taagatcttt
     4501 acaatctctc caaaataaa
//
LOCUS       DAONUO010000162         4515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13761_length_4515_cov_3.915695, whole genome shotgun sequence.
ACCESSION   DAONUO010000162 DAONUO010000000
VERSION     DAONUO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13761_length_4515_cov_3.915695"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /locus_tag="PK768_06655"
     CDS             <1..2142
                     /locus_tag="PK768_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HQA60688.1"
                     /translation="ATCFPSASTTASSFDPAVTECIGRAIGVEALAQKVGVVLGPGAN
                     LKRNPLCGRNFEYFSEDPYLSGKMAAGWIRGLEGVVVASCLKHFACNNQEKSRFNSDS
                     ILDERTLREMYLSGFEIAVKEGKPSSVMSAYNKINGVYCSDNRELLTDILRNEWGFDG
                     FVVTDWGGMSDRIAAFRAGCDLLMPGGSGYMEKDTIKAVRAGLLDEKEIDKSVERILK
                     QVFEAHQILNTPHTYNAEEHNRIAREAAEKSAVLLKNDLAILPLNEEQRIVLIGSMAK
                     EMRYQGTGSSHINPFRITTPCDAMPNLKWHEGCLDDGSTTNELVKDAVAAAKNADVVV
                     IFAGLPDNYESEGFDRSSMKMPDGQLRLIDEVAEANPNTVVVFFSGCALECPWADKVK
                     AILFMGLPGQAGGEAVKNLLFGKAVPSGKLSESWPVRYDDCASSNIYAKTKDALYVEG
                     IYVGYRYYEKAGIPVRFPFGYGLSYTSFVYSDLKVVGRTVSVTVTNTGKMSGAEAVLL
                     WVHPPQNGIHRPVRELKAFDKVYLQAGESKTVTFVLNDRSFAIWQNGWKVPGGNYKIS
                     VADLTSEIYVDGETVDVPEWQKESWYQNCNGKPSRSDLEKALGHEIVLSVSKKGEYTM
                     DNSIMEMKETSVIMKIMFRAIESTIAKQFNGKKDYANPEFRMMINSSAGAPLRSMQIS
                     AGIKDGLFEGLLEMANGSFFRGISKIMKSLI"
     gene            complement(2507..3352)
                     /locus_tag="PK768_06660"
     CDS             complement(2507..3352)
                     /locus_tag="PK768_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60689.1"
                     /translation="MKSRKIWVISIVSIISIIIVLIAGAIIRHNIKLKKYTEYLKDKY
                     GVSDSEIRFKSYKKEHNVYEGDFYGPYKRKVPSIITYEVNDRTVKVVDNGTTICDDYQ
                     IKDISYIAAEHFSKITGYNVEFVEFKSNSRYGNGFSDSLSEYMQTKNNSVITLDNIEN
                     FLVGYKNFDPNLFTSINLFINIASYDDLDTLVKDIDAKLFPYIKRTGLYNMGIWLYQG
                     NGQKLAITRSSLRNSKILPHYFCNYYVGDLDNIIAYLFCGTKKNFRQVGNFSVDERGI
                     WFFSR"
     gene            3600..>4515
                     /locus_tag="PK768_06665"
     CDS             3600..>4515
                     /locus_tag="PK768_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60690.1"
                     /translation="MSTKKIIHIVPSTHWDREWYLPFRRYQIRLVRLIEKVIKLLHDD
                     NYPNYLLDGQSIMLEDYLEIKPEDKNILKELISKGRLVPGPWYTVPDMMIPSGESLVR
                     NLLIGYSVCKEFGGCLKVGYSPDSFGLVSQLPQIYKLFGYKYAMFSRGLRLDSGKDTA
                     EFIWEAPDKSSVYALFEAYSIGVGITIPSVWRSYDRIDMSKEQVINSFKATMAYQDNR
                     YKGRNRLLVVGIDHLEPNDNLTETIATLKEEFKDEYEIKESTLEEFFESMVEELGNQL
                     ETGYGEQRGEYKSHFALGNTLSTRIDIKM"
BASE COUNT         1410 a          777 c         1003 g         1325 t
ORIGIN      
        1 gcgacctgtt ttccttctgc atctacaaca gcctcaagct ttgaccctgc cgtgacagaa
       61 tgcatcgggc gtgctattgg ggtagaggct ttggcacaga aagttggagt tgttcttggt
      121 ccgggtgcga atctgaaacg aaatcctttg tgcggcagaa attttgaata tttcagtgaa
      181 gatccatacc tctcaggaaa aatggcagca gggtggataa gaggtcttga aggggtcgta
      241 gtggcttcat gtctcaagca ctttgcctgc aacaatcagg aaaaaagtcg atttaactca
      301 gacagcattc ttgatgaacg cactttaagg gagatgtatt tatcggggtt tgaaatagcg
      361 gtaaaagaag gcaaaccttc ctctgtgatg tcagcgtata acaaaataaa tggggtttac
      421 tgctctgaca accgggaact gcttacagat attctccgta atgaatgggg atttgatgga
      481 tttgttgtta ccgactgggg cggaatgtca gatagaatcg cagcttttcg tgcaggatgc
      541 gatttgttaa tgcctggcgg cagcggttat atggaaaagg acaccatcaa ggctgttcgt
      601 gctggcctgc ttgacgaaaa ggaaattgat aaatctgttg agagaattct aaagcaagta
      661 tttgaagcgc atcaaattct aaacacacct catacctata atgcggagga acacaaccgc
      721 attgcgcgtg aggcagcgga aaaaagtgca gtcctgctga aaaacgattt agctatttta
      781 cctttgaacg aggagcaaag gattgttctt atcggttcaa tggcaaagga aatgcgttat
      841 cagggtactg gttcaagcca tattaatccc tttagaataa caacaccatg cgatgcaatg
      901 ccgaatctta aatggcatga gggatgtctt gacgatggtt ctacgacaaa tgagcttgtt
      961 aaagatgcgg tcgctgccgc taaaaatgcc gatgtggtgg tgatttttgc aggacttccc
     1021 gacaattatg aatcagaagg ctttgaccgc agcagcatga aaatgcccga cggccagttg
     1081 cgcttgatcg atgaagtagc agaagcaaat cccaatacgg tagttgtttt tttcagcgga
     1141 tgtgccttgg aatgtccctg ggcagacaag gttaaagcta ttttgtttat ggggcttccc
     1201 gggcaggcag gcggtgaagc tgttaagaat ctgctgtttg gtaaagcggt tccgtcagga
     1261 aagctatccg agagctggcc tgtacgttat gatgactgtg cctcatccaa tatttatgcc
     1321 aaaacaaaag acgctctata tgtagagggt atctacgttg gctaccgcta ttatgaaaaa
     1381 gcgggcatac ctgttcgttt cccctttggg tacggtttgt cgtacacttc ctttgtgtat
     1441 tcggacctga aggttgttgg caggaccgtt tcggttacgg taacaaacac cggcaaaatg
     1501 tcaggagcgg aagcggtatt gttgtgggtg catcctccac aaaacggcat tcaccgacca
     1561 gtgagagaat tgaaagcttt tgataaagtt tacctgcagg ccggggagag taaaactgtc
     1621 acttttgtac tgaacgaccg ctcgtttgca atttggcaga atggatggaa ggtgcctggc
     1681 gggaattaca agatttcagt tgctgatttg acttcagaga tctatgttga tggagaaaca
     1741 gtggatgtac ctgaatggca gaaagaaagc tggtatcaga actgtaatgg aaagccctct
     1801 cggagcgacc tcgagaaagc tttggggcat gaaattgtat tatcagtatc gaaaaaaggg
     1861 gagtacacaa tggataattc gattatggaa atgaaagaaa cctccgtgat catgaaaatc
     1921 atgttccggg caattgagtc aacaatcgcc aaacagttta acggaaaaaa agactatgcc
     1981 aatcctgaat tccgtatgat gataaactcc agtgcaggag ctcctcttcg cagtatgcaa
     2041 atctcagctg gtataaaaga cgggctgttt gagggactgc ttgagatggc aaacggaagt
     2101 tttttcagag gaataagcaa gataatgaaa agtttgatat gaaggtcgga tattgtattt
     2161 tggctataaa tggattatac tgtagtttga ttaattcgtt tactttgtca tgctgagaag
     2221 gagagattta tgaataaaac tagagtaata attgaatgtg cggttcttgc cttattttca
     2281 tttatattat tttatgtggt gatgtcaacg agattagtgg cttccataat attgtcatta
     2341 tttatttttg ctgcttgcat gataagacaa tacattaagt acaaaatgat aaaggaagaa
     2401 gaaaagtaca aataattcaa tattgcgatc ttaaactaca gggggaagaa gtagtataaa
     2461 caagaatatg aaaaatcctg ccgagaaatc gacaggattt tttgatttat ctgctaaaaa
     2521 accaaattcc tctttcatct acgctgaaat tgcctacttg tctgaaattc tttttagtac
     2581 cgcaaaataa gtaagctatt atgttatcaa gatcgcctac ataatagttg cagaagtaat
     2641 gaggtagaat tttgctgttc ctaagggaac ttcttgtgat tgctagtttc tgtccatttc
     2701 cttgatatag ccaaattccc atgttataaa gacctgtcct tttaatatag ggaaaaagtt
     2761 ttgcatcaat atctttaacc aacgtgtcaa gatcatcata actagctata tttataaata
     2821 aattaataga ggtaaataaa ttcggatcaa aattcttata gccaacaaga aaattctcaa
     2881 tgttatcaag agtaatgacg gaattgttct tggtttgcat atattcagac aaggaatcgc
     2941 taaatccatt accatatctg ctattactct taaactcaac aaactccacg ttataaccgg
     3001 ttattttaga aaaatgctct gctgcaatat aactgatatc tttaatttga tagtcatcgc
     3061 aaatagtagt gccattatct acaactttga cagttctgtc atttacttca tatgtaatga
     3121 tacttggaac ttttcgttta taaggaccat aaaaatcacc ttcatatacg ttatgttctt
     3181 ttttatacga tttgaatcga atttcgctgt cactcacacc atatttatct ttcaggtatt
     3241 cagtatattt ttttagttta atattatggc gaataatggc ccctgcaatc agcactatta
     3301 ttatagaaat aattgataca atggatataa cccatatttt ccttgatttc aaaatgttca
     3361 ccgtcccata tataatttta ctaataataa cgctggaaat tactggtgtc aacaagaaaa
     3421 aaacattcga tattacttgt catatgatgc tgataaaatg acagtatttg tctgatctgc
     3481 gatacgagtc gctaaaaaag aggtataacg tttccataat tagtactcca aataaagcca
     3541 tattattgac gttaatgata ctaaatgata acatatcaaa aaaacagtga aggtgaatca
     3601 tgagtactaa aaagatcata cacattgttc ctagtacaca ttgggataga gaatggtatt
     3661 tgccattccg cagatatcag ataaggttgg ttcgtcttat tgaaaaggtg ataaaactat
     3721 tgcatgacga caactatcct aattatttgc tggatggtca aagcataatg ttggaagact
     3781 atttggaaat aaaaccagag gacaaaaaca ttttaaaaga gttaatcagc aagggcagac
     3841 ttgtacccgg tccctggtac accgttcccg atatgatgat tccttctggt gaatctttgg
     3901 ttcgcaatct tctaattggg tatagtgttt gtaaagagtt tgggggatgc cttaaagttg
     3961 gttacagtcc ggacagcttt ggcttggttt ctcagctgcc gcaaatatat aagttgtttg
     4021 gatacaagta tgccatgttt tcaagggggc ttagactaga ctctggcaaa gacaccgccg
     4081 aatttatatg ggaagctcct gataaaagct cggtttatgc tttgtttgaa gcatacagca
     4141 taggtgttgg cataactatt ccttctgtct ggagaagtta tgaccgtata gatatgagca
     4201 aagagcaggt aataaatagc ttcaaggcca ctatggccta tcaggataac aggtataaag
     4261 gacgaaaccg actgctggta gttggaatag atcatttgga accaaatgac aatctgactg
     4321 aaactattgc tactctgaaa gaggagttta aagatgagta tgaaataaaa gaaagtacac
     4381 ttgaagaatt ctttgagtct atggtggaag aactgggcaa tcagctggaa acgggatatg
     4441 gagagcagag aggtgaatac aagagtcact ttgcgttagg aaacacttta tccaccagaa
     4501 tcgatatcaa gatgt
//
LOCUS       DAONUO010000163         4495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13833_length_4495_cov_3.133333, whole genome shotgun sequence.
ACCESSION   DAONUO010000163 DAONUO010000000
VERSION     DAONUO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13833_length_4495_cov_3.133333"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /gene="eno"
                     /locus_tag="PK768_06670"
     CDS             <1..1239
                     /gene="eno"
                     /locus_tag="PK768_06670"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQA60691.1"
                     /translation="GNPTVEVEVVLEDGTVGRAAVPSGASTGQFEAVELRDKDPKRYV
                     GKGVLNAVKNVNEEIAPELQGLDALDQVAIDKLLIEMDNTPNKGRLGANAILGVSLAT
                     AHAAANFLGISLYRYIGGSNAKVLPVPMMNILNGGEHADNNVDLQEFMIMPAGAKSFA
                     QALQMGAETFHTLKKVLGERGLSTSVGDEGGFAPNLSSNEEAIKYIIEAIEKAGYVPG
                     QDIFIALDAASSEFYNDGKYQLKGEGLEYDAEGMVNYLSRLVEKYPIISIEDGMAEED
                     WEGWKLLTDAIGDKVQLVGDDLFVTNTERLKRGIEKGVGNSILIKLNQIGTLTETLDA
                     ISMAERAKYTAVISHRSGETEDVTIADLAVATNAGQIKTGAPSRTDRVAKYNQLLRIE
                     QELGSTAQYLGISAFYNIKR"
     gene            1802..4270
                     /locus_tag="PK768_06675"
     CDS             1802..4270
                     /locus_tag="PK768_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQA60692.1"
                     /translation="MKELIVYPTPHLVLQHRMRQLRGKKGVAGEEPITYEIFVDRCIK
                     HVVASKMFMGDFKKNLVLKNIIQKLKIQNKLKYFDTIRQGYVQRIGEVIGELKQQDID
                     VDTFDAMISNKPCHNDLSLIYRAYQEFLSENHFYDQEDRYILCEEHVLESSFITGIDK
                     VHFKEFYSLSPIQQKIVDALGDKAVLSNSKLESNMKEIRVVRAHNRRTEVVNLAQVIL
                     EDLGEGLLPHNLCIVLRERELYEHILLEVFQEMGIPICLQLQAQLLHNPFIKALLKFL
                     QGGGSEYFPGEVLDESIQSKKSSIMEWVAFVVDFLERKGLPTGFCDVHSKNLFLLKRD
                     LNAFEAMVNLFDELIDVGQIFSDECVDLQDFIANLNMYLGGRYYTYRESSEGVLVLSP
                     AMLRGLKFDKVYVPGMVEGEFPRDFRPDWLLKDWERAAFNEKGYAFDTLDILLEKEEE
                     AFDFILTSARAGYFSYPNLADDNSAVLMSSYLEKLLGTYATSIENLRLESTYNFEGRD
                     YPVPEPGVISDITRDKLGEYFKERPFSATAFNMYGECPYKFFLARVLNLSPPEEEGEY
                     TALARGTVLHKILETFFKNHSEGLEANKLDEYVDEIKALTHDIMVGHNLKENFLHPLL
                     FEIEKNQIAESIINYIASYLKQAGDFKPIHFEMGFGYEREFAFDFAPDILFSGKIDRI
                     DEDPKGRLVVFDYKSGSTPDIKQIEEGTNLQMPLYIMACEQLLKQPVAGGAFISLKKA
                     DVDNILVRDRDLPFVSKRRKKGILSQEQWDELMTDVKSIIREYADNIRAAHFPIQPKK
                     CPKTDVFGSFCDFIAICPFEGVEE"
     gene            4267..>4495
                     /locus_tag="PK768_06680"
     CDS             4267..>4495
                     /locus_tag="PK768_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQA60693.1"
                     /translation="MKYTPEQQKAIVTVDKDLAVTAGAGAGKTRVLVDRILYILKNGL
                     ADIDEIVAITYTNKAALEIRERLRKEMLKQKG"
BASE COUNT         1401 a          841 c         1052 g         1201 t
ORIGIN      
        1 ggtaatccaa ctgtagaagt agaagttgta ctagaagatg gtaccgtagg aagagcagca
       61 gttccctcgg gtgcatctac gggccagttt gaagctgtag agctgagaga caaggatccc
      121 aagcgctatg taggcaaggg cgttctaaat gcagtgaaaa atgtcaatga agaaattgcc
      181 cctgaacttc agggattgga tgcattagat caggtggcca ttgataaact cttaattgaa
      241 atggataata cgccaaacaa gggtcgctta ggcgccaatg ccatcttggg tgtatccctg
      301 gccacagctc acgcggcagc caatttcttg ggaataagcc tttacagata tatcggcggt
      361 tcaaatgcca aggttttgcc ggttcccatg atgaatatat taaacggcgg cgagcatgcg
      421 gataacaacg tagacctgca ggagttcatg attatgccgg caggcgccaa aagctttgca
      481 caggcccttc aaatgggagc cgaaacattc catacattaa agaaggtttt aggagaaaga
      541 ggcctttcta cttcagtagg cgatgaggga ggctttgctc caaacctatc ttccaatgaa
      601 gaggcgatca aatacattat tgaggcaatc gaaaaagccg gttatgttcc cggccaggac
      661 atctttatag cactggatgc tgcttcatca gagttttaca atgatggcaa atatcagcta
      721 aaaggagaag gcttggaata cgatgcagag ggcatggtaa actatctgtc ccgcttagtg
      781 gagaaatacc ccataatatc cattgaagac ggtatggccg aggaagattg ggaaggctgg
      841 aagctcctta ccgatgccat cggcgacaag gtgcagctgg taggtgacga cctgtttgtc
      901 acaaatacgg agcggttaaa gcggggtatt gaaaaaggag tgggcaactc catacttata
      961 aaactaaacc aaatcggtac actcacagaa actctagatg caatttccat ggctgaaagg
     1021 gccaaataca cggcagttat atcacaccgc tccggtgaaa cggaagacgt aacaatagca
     1081 gacctggcag tggctacgaa cgccggacaa atcaagaccg gtgcaccctc ccgtacggac
     1141 agagttgcca aatacaatca gctcctcaga attgaacagg aactgggtag cacggcacag
     1201 tacctcggca tttcagcttt ctacaacatt aagcgctaat tgaatttccg cattactttg
     1261 taagttaacc catgatttgt ctatagatag tgcaatttct cctgatggaa agtttcttgt
     1321 gagttatctg tcacaaaaac tttccatcgg ttttcttaag actattaatt gttatccgat
     1381 aatataatag ctttatatga attaacttta atgttactaa tggttgaaag attaagcacc
     1441 ctattcaact aaatttcaaa gctgttgatt cttgttggta atactgaatt tttctcaatt
     1501 tgtacttagc aactacgaag gttgtcaggt aatacaagtg agcgaatttt attttttcgt
     1561 tttggttctt gatgagattt caagaaagcc gtaaaaagct tttcgcggag taacaacaat
     1621 gatattatag cgaggcttaa ataattctac gcataatttc ttttttgcaa ataaaaatga
     1681 tcttaaaagc tttaggcttt tgtagaaatc gaatctagaa ccatagaaaa aataaacctg
     1741 agcgaactgt attacctgac agtatatgtt gttctataga aagcttttta gggggccttt
     1801 tatgaaagaa ctaattgtat accccacgcc gcatcttgta ctacagcata gaatgaggca
     1861 gctccggggc aaaaaagggg ttgccggaga agagccaatt acctacgaaa tctttgttga
     1921 taggtgcata aagcatgttg tagcttcgaa gatgtttatg ggtgatttca agaaaaactt
     1981 ggtcttaaaa aatataatcc aaaaactgaa aatacaaaac aaactaaaat attttgatac
     2041 tatcaggcag ggttatgtac agcgcatcgg ggaagtgatt ggtgaactaa agcagcagga
     2101 tatagatgta gatacttttg atgccatgat tagcaacaag ccttgtcata acgacctgtc
     2161 attaatctac agggcatacc aggagtttct tagcgagaac catttttatg atcaggaaga
     2221 taggtacata ctgtgcgaag aacatgttct agaaagcagc tttattacgg gcatagacaa
     2281 agttcatttt aaggagtttt atagcctatc tccgattcag cagaaaattg tagatgcact
     2341 tggagataag gctgtcctta gcaattcgaa gctggaatct aatatgaaag aaataagagt
     2401 cgtaagagca cacaatcgga gaacagaggt tgtaaacctt gcccaggtga tactggaaga
     2461 cttaggggaa gggctcttgc cccacaacct atgcatagtc ctgcgagagc gggaacttta
     2521 cgagcatatt ttgttggaag ttttccagga gatggggatt cctatttgcc tgcagttaca
     2581 ggctcaactc ctgcacaacc cctttataaa ggcactgctg aagtttttgc agggcggggg
     2641 cagcgagtat tttcctggag aagtgcttga tgaaagcatc caaagcaaaa aatcttccat
     2701 tatggaatgg gtagcttttg tggtagactt ccttgagaga aagggcctac ctacaggatt
     2761 ctgtgatgta catagcaaaa acctcttctt attaaaaagg gatttaaacg cctttgaagc
     2821 catggttaat ctttttgatg aattaataga tgtaggccaa atattctccg atgagtgtgt
     2881 agacctgcaa gactttatag caaatttaaa catgtacctc ggcggtcgat attacactta
     2941 cagggaatcg agtgagggag tattggtgct gtctccagcg atgctcaggg gcctcaagtt
     3001 cgataaggtt tatgtcccgg gaatggtgga aggtgaattc ccgagagatt ttaggcctga
     3061 ttggcttttg aaggactggg aaagggcagc ttttaatgaa aaggggtatg cttttgatac
     3121 cttagatatc ctccttgaaa aagaagagga agcctttgac tttattttaa catcggcaag
     3181 ggcagggtat ttttcatatc ccaatttagc agatgacaac agcgccgtgc tgatgtcatc
     3241 gtaccttgaa aagctgctcg gcacatatgc gacttcaata gaaaacctaa gattagagtc
     3301 aacatataat tttgaaggca gagactatcc tgtacccgag ccgggagtca tttcggacat
     3361 tacaagagat aagctaggcg agtattttaa ggagagaccc tttagtgcaa cggcttttaa
     3421 tatgtacgga gaatgtccct acaagttttt cctagcgaga gtcttgaacc tgtctcctcc
     3481 cgaggaagaa ggcgaataca cagcccttgc cagggggacc gttttgcaca aaatacttga
     3541 gacttttttt aaaaatcaca gcgaaggcct tgaagctaat aaattagatg agtatgttga
     3601 tgaaataaaa gctcttaccc atgatatcat ggtaggccat aacttgaagg aaaacttttt
     3661 gcacccattg ctgtttgaaa tagaaaagaa tcaaattgcc gaaagcatca ttaattacat
     3721 agcttcatat ttaaaacagg caggagattt taaacctatt cattttgaga tgggctttgg
     3781 ttatgagcgg gagtttgctt ttgattttgc ccccgatatt ttattttccg gcaaaattga
     3841 tagaatagat gaagacccaa agggcaggtt agttgttttc gattataagt cgggctcgac
     3901 tcccgatata aaacagatag aagaaggcac aaatcttcaa atgcctctct acataatggc
     3961 atgtgaacag ttgcttaaac agcccgtggc aggaggagcc tttatttccc taaaaaaagc
     4021 tgatgtggac aatatcctgg taagggatag agatttgcct tttgtatcaa agcgccggaa
     4081 aaaggggatt ttgagccagg agcaatggga tgagctgatg actgacgtaa aaagcatcat
     4141 tagagagtat gcagacaata taagggcagc tcattttccg atccagccaa aaaagtgccc
     4201 caaaacagat gtttttggca gcttttgcga ctttatcgca atctgtccct ttgagggggt
     4261 ggaggaatga agtacacacc ggaacagcaa aaggctattg taaccgttga caaggatctg
     4321 gccgttactg caggagcagg ggcaggcaag acgagagttt tagttgaccg cattttatat
     4381 attcttaaaa atggtcttgc agatattgat gaaatagttg caattaccta caccaataaa
     4441 gcagccctgg aaataaggga acggcttcgc aaagaaatgc tcaagcaaaa aggcg
//
LOCUS       DAONUO010000164         4489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13850_length_4489_cov_3.962562, whole genome shotgun sequence.
ACCESSION   DAONUO010000164 DAONUO010000000
VERSION     DAONUO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13850_length_4489_cov_3.962562"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PK768_06685"
     CDS             <1..623
                     /locus_tag="PK768_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HQA60694.1"
                     /translation="QNKEQKGASFRPDVVEVTVSIVPVSNVQVLPNISGNPPEGYIIR
                     DVRIDPPTVVVTGGHDVLEGLQSISTEAINVEGATSTVSTDAKLIIPKGVTIFDEGIQ
                     AARVTVEIERLATTSISVSSDEIQVLNIPSDLEASLERREILLTVSGPESIIDKVNKN
                     MINLNIDAGGLLEGEHLLGVRADITRPYRIIKIEPAEIAVTLRKLS"
     gene            643..1980
                     /gene="glmM"
                     /locus_tag="PK768_06690"
     CDS             643..1980
                     /gene="glmM"
                     /locus_tag="PK768_06690"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQA60695.1"
                     /translation="MGRLFGTDGVRGIANTELSPLLAFKLGRAGAKVLSKLQKRPLFV
                     VGKDTRISGDMLEAALTAGICSAGADVIKVGIVPTPAVAYLTRFFNADAGVVISASHN
                     PVEYNGIKFFDKNGYKLPDAMEDDIEALLDDANQAIESPTGSDVGKIIEKGGVRPYVD
                     FIKSAVSKDFSDLTVALDCANGASYEVAPVIFEELGAKVHVINNTPDGCNINANCGST
                     HPDALCQFVREVGADVGLAFDGDADRLIAVDEKGNIVDGDHVMAICGCHMKRQGLLKN
                     DTVVSTIMSNMGLEVALKKAGINMVRTKVGDRYVLEEMLSSGYNLGGEQSGHIIFLDH
                     NTTGDGILTAVNLLKVMVDEQKGLSDLSSIMTVYPQVLINVRVADKSKYDNNERIKNT
                     IDEGEALLGDSGRIVVRPSGTEPLIRVMVEGEQEDLINKVADNVAKVIEEELK"
     gene            2186..2716
                     /locus_tag="PK768_06695"
     CDS             2186..2716
                     /locus_tag="PK768_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HQA60696.1"
                     /translation="MDKNLWDKCVEFHGHECPGLAIGFKACEAAMKKLGISFSKDEEV
                     VCVTENDACGVDAVQVITGCTIGKGNLIYRGTGKQAFSFFCRDTGERLRIVFKADSLS
                     KDLSREERQQAILESPVEKLFEFKEPGFDLPERARIFNTLTCSKCGEGAPEHKMRLAE
                     GKAVCLDCFDDFSRGW"
     gene            2719..3516
                     /locus_tag="PK768_06700"
     CDS             2719..3516
                     /locus_tag="PK768_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQA60697.1"
                     /translation="MEILFDRFWKMEDKNNKNQQQFWDLRADEFNSRMGSQRTMKEVE
                     EVLKYLKDKGAIDEKGKVLDIGCGPGKYTLAFAKEVICVIGTDISSKMISFAKENARS
                     RGLTNVNFFQASWPEVNIEELGWKKNFDLVFAAFCPGIDGPDALKKMVEASSKHCFLS
                     GFVSRQDQVLEGLKKHLGIQGKTWGNQVYYSFNLLWQWGYYPEITYQDRAWTNEYDVQ
                     QMADILVSRLDGQASKEDIMDYLKTVSVNGKVTEHSRSKIAWLYWQV"
     gene            3594..>4489
                     /locus_tag="PK768_06705"
     CDS             3594..>4489
                     /locus_tag="PK768_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA60698.1"
                     /translation="MKNTIIRKLLIMFILAAVILTFTGCGSKPEQSTADKIEEQESSP
                     LVIRLEGGDWGFPSPFAHYSRGPGMSKMRLIYDSLLEKGEEGLIPWLAKDWSISPDGK
                     EYSFVLNPGVKWHDGKMLTAEDVKFSFEYFLKHPPVSNSLLIDGKPFIEKVETIGEDQ
                     VKIVVSEPNATVLDRLGIVRIIPKHIWESIDDPKKMLDIDKLVGCGPYILTDYSQEQG
                     AYRFMAFEDYWGPKPLVDLVEFVPVSDPVLAFENGEIDLIKVPQDLMDRYADNKEYKI
                     LKSPAFWGYKLMFNMEKKPELL"
BASE COUNT         1356 a          874 c         1163 g         1096 t
ORIGIN      
        1 cgcagaacaa ggagcaaaag ggagcaagct ttagacctga tgtggtagaa gtgaccgtat
       61 ccattgttcc cgtcagcaat gtgcaggttt tgcctaatat aagtggcaat ccacctgaag
      121 gttatataat aagggatgtg aggatagacc cgcctaccgt agtggtgacc ggaggccatg
      181 acgtgctgga aggcctccag tcaataagca ccgaagccat aaatgtggaa ggggccacat
      241 caacggtatc aacagacgca aagcttataa tacccaaggg cgttacaatc tttgatgaag
      301 gaattcaagc agcccgggtt accgttgaaa tcgagcggct ggccacgaca agtattagcg
      361 tcagctcgga tgaaatacag gtactaaaca taccctcgga tttagaggcg agcctggaac
      421 gcagggaaat actattgaca gttagcggtc ccgagagtat tattgataaa gtaaataaaa
      481 acatgattaa cttaaatatt gatgctggcg gccttttgga aggtgaacac ttgctgggcg
      541 tacgtgcgga cataacgcga ccttacagga ttataaaaat tgaaccggca gaaattgcgg
      601 tgacacttcg caaactttct tagtatggag aggagaaaac atatgggcag actatttgga
      661 accgatggag ttaggggtat tgcaaataca gagttgtctc ccctattggc ttttaagttg
      721 ggccgtgcag gtgccaaagt attatccaaa cttcaaaaaa gacccctctt tgtcgttggc
      781 aaggacacac gcatatcggg agatatgcta gaagcggctt tgacagcggg aatatgttcg
      841 gcgggtgccg acgtgattaa agtgggaatc gtaccgacac ctgcagtggc ttacctcacc
      901 cgctttttca atgctgatgc aggtgtagta atatccgcat cccataatcc tgtagaatac
      961 aatggaataa agttctttga taaaaatggt tacaaattgc ccgatgcgat ggaagatgat
     1021 atagaagctt tacttgatga tgccaatcag gcaatagaaa gccctacagg gtcagatgtc
     1081 gggaagatta ttgaaaaagg cggagtaagg ccttatgtgg attttataaa gtccgcggtc
     1141 agcaaggact ttagtgactt aaccgttgcc ctggattgcg caaatggtgc atcgtacgaa
     1201 gtggcacctg tcatctttga agaacttgga gctaaagtcc atgtaattaa taatacgcct
     1261 gatggatgca atatcaatgc taattgcggt tccacccatc ccgatgccct ttgccagttt
     1321 gtaagagaag ttggtgcgga tgtgggtctt gcctttgatg gcgatgcgga caggctcata
     1381 gcagtcgatg aaaagggcaa tatcgtggac ggcgaccatg tgatggcaat ctgcggctgc
     1441 catatgaaaa ggcaggggct tttaaaaaat gacaccgtag tttccactat aatgagcaac
     1501 atgggcttag aggttgccct taaaaaagcc ggaataaaca tggtgcggac aaaagtggga
     1561 gaccgttatg ttctcgagga aatgctaagc agcggctaca atctaggtgg agaacagtcg
     1621 ggacatataa tattcttaga ccataatact acaggtgacg ggattttgac cgctgtaaat
     1681 ctattaaaag tgatggttga tgagcaaaaa ggcctatcgg atttaagcag cattatgacc
     1741 gtttatcccc aggtgcttat aaatgtccgc gtagccgata agtccaaata tgataacaat
     1801 gagcgcatca agaatactat agatgaaggt gaggccttac ttggcgacag cggcagaatt
     1861 gtggtccggc catcgggcac ggaaccatta atccgcgtca tggtggaagg agaacaggag
     1921 gacttgatta acaaagttgc cgacaacgtt gccaaggtaa tagaagagga attgaagtga
     1981 tgaatatttg gggacagtcc ccaaatattc attgacagtc cgaaataacg gtgttaaaat
     2041 attatcaaag accatgaagg tctatattta ctccaagtaa gatagaatta aattgactga
     2101 agaaaaacca tgaaggttaa gctgtatgaa acggcttatt tcatggtttt tattttttat
     2161 caattagcat tagaggagga gaataatgga caaaaacctt tgggataaat gcgtagagtt
     2221 tcacggccat gagtgtccgg gtctggccat cggctttaaa gcttgtgaag ctgccatgaa
     2281 aaaactgggc ataagctttt caaaggatga ggaagtcgtt tgcgtaactg aaaacgatgc
     2341 ctgcggcgtt gatgcggtcc aggttatcac cggctgcacg ataggtaagg ggaatcttat
     2401 atatcggggc acgggcaaac aggcctttag cttcttctgc cgggataccg gggaaaggct
     2461 gcgaatagta ttcaaagctg acagtctgag caaggacctg tctagagaag agcggcaaca
     2521 ggcaatcttg gaaagtcccg tggaaaagtt gtttgaattt aaggaacccg gttttgactt
     2581 gccggagagg gcacggattt tcaatacctt gacatgcagc aagtgcggcg aaggtgctcc
     2641 cgaacacaaa atgcgccttg cggaaggtaa ggccgtatgc ttggactgct ttgatgattt
     2701 ttcaagaggc tggtagccat ggaaatcctc tttgaccgat tctggaaaat ggaagacaaa
     2761 aacaacaaga atcaacaaca gttctgggac cttagagccg atgagtttaa ttcccgcatg
     2821 ggttcgcaaa ggaccatgaa ggaagtggag gaagtcctga aatatttgaa ggataaggga
     2881 gcaattgacg aaaagggcaa agtcttagac atcggctgcg ggcccggaaa atacacccta
     2941 gcttttgcca aagaggtaat atgtgttatc ggaaccgata tttctagcaa gatgataagc
     3001 tttgccaaag aaaatgcccg aagcaggggt ttaacgaatg ttaatttctt ccaagcttca
     3061 tggcccgaag taaatataga agagctaggc tggaagaaga actttgacct tgtatttgcc
     3121 gccttttgcc cggggattga cggtcccgat gctttaaaga aaatggttga ggcaagctcc
     3181 aagcactgct ttctcagcgg ctttgtgtca aggcaagacc aggtcctaga aggcttgaaa
     3241 aaacatcttg gaatacaggg caagacctgg ggcaatcaag tctactatag cttcaatcta
     3301 ctttggcagt ggggttatta tcccgaaatt acatatcaag acagggcctg gacgaacgaa
     3361 tatgacgtac agcaaatggc agacatttta gtttcaagac ttgatggcca ggcaagcaaa
     3421 gaagatataa tggattatct caagaccgtt tccgtaaacg gaaaagtgac ggaacacagc
     3481 aggtcgaaga ttgcatggtt atattggcaa gtttaggctg aattcaccat cagaaattca
     3541 attctacaac agtgtcttgt tccgacgaca atactgatta agaaggggtt catatgaaaa
     3601 atacgattat acgaaaactg ctcattatgt ttatcctagc agccgttatc cttactttta
     3661 ccggctgcgg ctcaaagccc gagcaaagta ccgcagacaa aatagaagag caggaatcct
     3721 ctcccctggt gattcgcctg gaaggcgggg actggggttt tccctccccc tttgcccact
     3781 attcaagggg gccgggcatg tcaaagatgc gcctcatata cgacagcctt ttggaaaaag
     3841 gtgaggaggg cctgattccc tggcttgcca aggactggag catatctccc gatggtaagg
     3901 agtacagttt tgtattgaat ccgggagtaa agtggcatga cggcaagatg ctaactgccg
     3961 aagatgtgaa attttccttt gagtattttt taaaacatcc cccggtttcc aacagcttgt
     4021 taatcgatgg aaagcccttt attgaaaaag tagaaactat tggtgaagac caggtcaaga
     4081 tagtggtaag cgagcccaat gccacggtgc ttgacagatt gggcattgtc aggattattc
     4141 ctaagcacat ttgggaatcc atagacgatc caaaaaagat gctggacata gacaaattgg
     4201 taggatgcgg tccttacata cttaccgatt acagccagga gcagggtgca taccgcttta
     4261 tggcctttga ggactattgg ggaccaaagc ctttggtcga cctagtagaa tttgtcccgg
     4321 taagtgaccc ggtgctggcc tttgaaaatg gcgaaattga tttaataaag gttccccagg
     4381 acctaatgga ccggtatgcc gataataaag aatataaaat attaaagagt ccggctttct
     4441 ggggctacaa gctgatgttt aacatggaga aaaagccgga actattaga
//
LOCUS       DAONUO010000165         4470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13920_length_4470_cov_3.918913, whole genome shotgun sequence.
ACCESSION   DAONUO010000165 DAONUO010000000
VERSION     DAONUO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13920_length_4470_cov_3.918913"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1479)
                     /locus_tag="PK768_06710"
     CDS             complement(190..1479)
                     /locus_tag="PK768_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HQA60699.1"
                     /translation="MKLSEKLRAFLSTKPKEKKDEKNLTTNFHKSLGDILGTIEISTP
                     FGEHLLVERSYPDSHIHGNVQLNLIYNISGEIIKLIAKCPDYEDLDFRRAVFIDTETT
                     GLAGGSGTLAFLIGVGFFEGDSFKVLQYFISDYDKEAAALYSLSNLLKKFDSLVTFNG
                     KSYDIPLLEARYMISRMESPFNKTFHLDLLSAARRLYKERLESVSLSSLEQNLLSLKR
                     EGDIPSYEIPSVYFRFLRDSNPYPLKPVFYHNRMDILSMVTLTISMANAFKEPLDTTS
                     CKNQDYYCLGRVFEDMGMVEESIECYKRALQVQGVREKTHAQLSLLYKRLGRWQEAER
                     LWMAMVEANIRPDFALVELAKYYEHRLKDYNRAAKAAQMALENVYKKNSLVGYTLKDE
                     IVELKKRLERIKCKQKKYETRSLEDHCFPRQESLHIN"
     gene            complement(1476..3734)
                     /locus_tag="PK768_06715"
     CDS             complement(1476..3734)
                     /locus_tag="PK768_06715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQA60700.1"
                     /translation="MNLEQVLDQLRTQERFYKNITHWEVVSESSGRYEDFPEYLNPKL
                     IKALNKKGIYRLYSHQRRALDEVMTGHDITVVTPTASGKSLCYNLPVLNTLLSDKDAR
                     AIYLFPTKALSQDQVSELMELVDDMDANIKTFTYDGDTPVNARVAIRKDGHIVVTNPD
                     MLHTGILPHHTKWMKLFTNLKYIVIDEVHIYRGIFGSHTANVLRRLRRICNFYGSNPV
                     FICCSATIANPGELSEKLTGKPAVLIDENGAPAGEKNIIFYNPPVVNRQLGIRRSSLL
                     EAKALASKFLNNKIQTITFARSRLAVEVLLTYLQEDMKKLPGGTNLVRGYRGGYLPKE
                     RREIEKGLREGKILGVVSTNALELGIDIGNLDASIITGYPGSVASTWQQAGRAGRRNS
                     VSASILVASSSPLDQFIINHPEYFFDATPEYGLINPDNLYILVSHIKCAAFELPFSDG
                     ETFGDQPINEILAFLEDEGILRHVGGRWHWMAESFPAEDISLRSASAENFVIIDITEG
                     AKVIGEVDRSSAPMLIHEEAIYIHAGQQYQVEKLDYEEKKAYVRKVNVDYYTDANLAV
                     EIKVLDMFREKTVANSQNYLGEVMVTSLATMFKKIKFFTHENLGFGPIHLPPEEMHTT
                     AYWISIPENTGGLSIEEIKSGLLGLCNVMVNIAPLYLMCDPKDIRGVVQIKSPFTQKP
                     TIFIYDNFPGGVGFAEKLYTMEDQLLKAARQVILECDCEDGCPSCVGPHEEVGHGSKD
                     FALKIIKGVLSD"
     gene            complement(3901..>4470)
                     /locus_tag="PK768_06720"
     CDS             complement(3901..>4470)
                     /locus_tag="PK768_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HQA60701.1"
                     /translation="GLAIFPAVFAFGFEPDAGPGLLFITIPMVFSKMPLGNILLTIFF
                     LLTAIAATTAMMSLLEVPVAYLVEEKGFSRPTATIMSAVTIALLGSTASLSADAGSLL
                     GTVKIFGKTFFDLYDYISSNILMPVGGLFTVLFIVWILGKKSLALELSNNGQLNNKGV
                     VNLFYTVVKYITPLLIIIIFLDSIGILKF"
BASE COUNT         1179 a         1036 c          934 g         1321 t
ORIGIN      
        1 ctcttaccgt gtttttcacc gtaggctgcc atttcttcgc ccagctttgc aagctctgct
       61 tcatcgtcaa taaccttaat ctctatgtag tcttttcccg cagttttggg agccgtcctg
      121 gctgataggg ccatgaattg tgccgccatc ttcaatgcat ctttcataaa aaatctcctc
      181 ctcataatat taatttatgt gtaagctttc ttgtcgagga aaacaatggt cctcaaggga
      241 cctagtttca tacttttttt gcttgcattt tatccgttca aggcgctttt taagctctac
      301 aatttcatct tttaatgtat atcccacaag gctattcttt ttatacacat tttccaatgc
      361 catttgagct gcctttgctg ccctgttata atctttgagc ctgtgttcat agtatttggc
      421 aagttctacc agtgcaaagt cgggccgaat attggcctct accatggcca tccaaagtct
      481 ttcagcctcc tgccagcgcc ccaatcgctt gtacagcagc gatagctggg cgtgggtctt
      541 ttccctgact ccctgtacct gcagggctct tttgtagcac tctatacttt cctcgaccat
      601 ccccatgtct tcaaaaaccc tgcctaaaca gtagtagtct tgatttttac agcttgtagt
      661 atctaacggc tctttaaagg cattggccat gcttattgta agggtaacca tagataagat
      721 atccattcgg ttgtggtaaa acacgggttt tagggggtaa ggattgctgt cccttaaaaa
      781 tctaaaatat acagatggga tttcatagct gggtatgtca ccttcccgtt taagggaaag
      841 gagattttgc tccagagatg ataagcttac actctccaag cgttccttat acagccttct
      901 tgccgcggac aagaggtcga ggtgaaaagt tttgttaaag gggctttcca tgcggcttat
      961 catgtaccgg gcttccagca atggtatatc gtaggatttg ccgttaaagg ttactagact
     1021 gtcaaatttt ttcagaaggt tacttaagct gtaaagggca gccgcctctt tatcgtagtc
     1081 gcttataaaa tactgtaaga ccttaaaact atcgccttca aaaaatccta cacctattaa
     1141 aaaagctaaa gtgccgctgc ctccggcaag gccggtagtc tccgtatcga tgaatactgc
     1201 tcgcctaaaa tccaagtcct cgtaatccgg gcatttggca atgagcttaa ttatttcccc
     1261 ggaaatattg taaatcaaat tcagctgaac gttcccatgg atatgggagt cgggatagga
     1321 cctctctacc aaaaggtgct caccgaaggg tgtagatatc tctatcgtcc caagtatatc
     1381 ccctaatgat ttgtgaaaat ttgttgttag atttttctca tccttttttt ccttaggctt
     1441 tgttgacaaa aaggccctca acttttccga aagcttcaat cggaaagcac ccctttaata
     1501 attttcagag cgaagtcttt gctgccatgg cccacttctt catggggtcc aacacaggat
     1561 gggcagccat cttcacagtc gcattccaat atgacttggc gtgcagcttt taataattgg
     1621 tcttccattg tgtagagctt ctcggcaaaa cccacgccgc caggaaagtt gtcgtagata
     1681 aagatggttg gcttttgggt aaagggtgat ttgatttgca caactccgcg aatatctttt
     1741 gggtcacaca ttaaatacag cggtgctatg tttaccatca cattgcaaag ccccaatagc
     1801 cccgacttta tctcttcaat cgaaaggcca cctgtgtttt cgggaatgga tatccagtaa
     1861 gctgtagtgt gcatttcttc aggaggcagg tggatggggc caaaacccaa gttctcatgg
     1921 gtaaaaaact ttatcttttt aaacatggtt gcaagacttg tcaccattac ttctcccagg
     1981 tagttttggc tattggccac tgttttttct ctaaacatgt ccaaaacctt tatttcaaca
     2041 gcaagattgg catcggtata gtaatctacg ttgacctttc gaacataggc ctttttctcc
     2101 tcgtagtcaa gcttttccac ctggtattgt tgtccggcat gaatgtaaat ggcttcttcg
     2161 tgaatcagca ttggagcgct gctgcggtca acctcgccga tgaccttggc gccctcggtg
     2221 atatcaataa tgacgaagtt ttccgccgat gcacttctaa gacttatgtc ttccgcagga
     2281 aaggattctg ccatccagtg ccaacgacct cccacatgcc gaaggatccc ttcatcttct
     2341 aaaaaggcca atatctcatt tatgggctgg tcgccgaaag tttcgccatc gctgaaaggc
     2401 agctcaaaag cggcgcattt tatgtgactt acaagaatgt aaaggttatc cggatttatt
     2461 aagccgtatt ccggtgtggc atcaaagaag tactccggat ggttgattat aaactggtca
     2521 agggggctac tggaagccac caaaattgaa gcagataccg agtttcttct gccagcacgg
     2581 cctgcctgct gccaggtgct ggctaccgac ccgggatagc ccgttattat actagcgtcc
     2641 aaattaccga tatctatgcc aagttccaag gcattggtgc tcaccacacc aaggattttt
     2701 ccctcacgaa gacccttctc tatttccctg cgctccttgg gcaggtaccc tccccgatat
     2761 ccccttacga gatttgttcc tccggggagc tttttcatgt cttcctgtaa ataggtaagg
     2821 agcacctcta ccgccaagcg gcttcgagca aatgttatag tttgaatctt attatttaaa
     2881 aactttgaag caagggcttt cgcttccagc aaactactcc tacgaattcc cagctgcctg
     2941 tttaccaccg gagggttgta gaatatgatg ttcttctcgc ctgcaggtgc accattttca
     3001 tcaattaaga cagcaggctt tcccgtcagt ttttccgaaa gctctcctgg atttgcaatg
     3061 gtagcagaac agcaaatgaa tacggggttt gaaccgtaaa aattgcatat gcgccttagt
     3121 cttctaagga cattcgcagt gtgacttccg aatattcccc ggtaaatgtg cacttcatct
     3181 ataactatgt actttaaatt ggtaaaaagt ttcatccact tggtatggtg aggaagtatt
     3241 ccggtgtgca gcatgtccgg attcgtgaca actatatggc catccttgcg aatagctact
     3301 cgtgcattga ccggggtgtc accatcatag gtgaatgtct ttatgtttgc gtccatatca
     3361 tctactaatt ccatcagttc agacacctgg tcctgggaga gagcctttgt gggaaaaaga
     3421 tatatggccc tggcatcttt gtcggaaagg agggtgttta agaccggaag attgtaacaa
     3481 agggacttac ccgaagcggt gggggtaacc acggtaatat catggccagt cataacctca
     3541 tccagagccc tccgctggtg agaataaagc ctgtatatgc ctttcttgtt taatgcttta
     3601 atcaactttg ggtttaagta ttcgggaaaa tcctcatacc tgcctgaact ttcagataca
     3661 acttcccagt gagttatatt tttgtaaaaa cgctcctgag tcctgagctg atccaaaact
     3721 tgctccagat tcatctgata tcccctctcc catgctttgt cctttattat agcataggaa
     3781 aaaacgtaaa aaaagctttg cagttatcca agcccccggc ttgaaagaac aattgtcttt
     3841 ataaaaaaag ccttgatgtt ttactcatca ggcttttttt gtataaggaa aatattaagc
     3901 ttaaaacttc agtatgccga tggaatctaa gaaaatgatg attatcagta aaggtgttat
     3961 atattttacg actgtgtaaa acaggtttac aacacctttg ttgttaagct gcccattgtt
     4021 ggaaagttcc aatgcaaggc tttttttacc caaaatccaa acaataaaga ggaccgtaaa
     4081 caaacctccc acgggcatta agatatttga agatatgtag tcataaaggt caaaaaacgt
     4141 cttgccgaaa atcttaacgg ttcccaagag cgaacccgca tctgctgaaa gggatgcggt
     4201 agaacccaag agggctattg tcacagcaga cattatagta gcagttggtc gagaaaagcc
     4261 cttttcctct accaggtaag caacaggcac ttcaagcaac gacatcatgg cagtggtggc
     4321 agctattgct gtcagcagga agaatattgt cagcaaaatg tttcctaaag gcatcttgct
     4381 aaaaaccata ggtatggtga taaataacag gcccggaccc gcgtcaggtt caaatccaaa
     4441 agcaaaaacc gccgggaata ttgcaaggcc
//
LOCUS       DAONUO010000166         4460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_13953_length_4460_cov_3.698297, whole genome shotgun sequence.
ACCESSION   DAONUO010000166 DAONUO010000000
VERSION     DAONUO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13953_length_4460_cov_3.698297"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /gene="rpoA"
                     /locus_tag="PK768_06725"
     CDS             <1..621
                     /gene="rpoA"
                     /locus_tag="PK768_06725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQA60702.1"
                     /translation="KDIIADADVEILNPDLHIATLEKDAKLFMELTLEKGRGYVTADR
                     NKKSNQPIGVIAIDSIFTPVHKVNFNVENTRVGQRTDYDKLTMEIWTNGAIKPDDAVS
                     TAAKILIDHFNLFLGLTDQVQTEEEPVETEKDDMQKILEMPIEELDLSVRSYNCLKRA
                     GINTIYELTQKTAEDMMKVRNLGKKSLQEVESKLAAFNLSLKQPDD"
     gene            637..972
                     /gene="rplQ"
                     /locus_tag="PK768_06730"
     CDS             637..972
                     /gene="rplQ"
                     /locus_tag="PK768_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQA60703.1"
                     /translation="MRKLGRPSGHRKMMLKNLTTDLFRYGRIETTETRAKEVRRIAEK
                     MITLAKKNDLAARRLVLAELLDETTVKNLFDKIAPKYQDRNGGYVRLTKIGLRRGDST
                     PLAVLELVE"
     gene            991..1827
                     /locus_tag="PK768_06735"
     CDS             991..1827
                     /locus_tag="PK768_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HQA60704.1"
                     /translation="MKITAEITAKNVYYQYSADSSLALNNINLTIKPGEFVAIIGPNG
                     SGKSTLAKLFNGIFVPTKGDVFVDGLNTKDKDKIWEIRRKAGMVFQNPDNQIVATVVE
                     EDVAFGPENLGIAPEEIRRRIDEALRTVELTEFANKAPHLLSGGQKQRVAIAGILAMK
                     PDCIILDEPTAMLDPVGRKEVISTVTKLNRQEGKTVILITHFMQEAVCADRVLVMEKG
                     RIVMEGKPNEIFSQVEKLKQYGLDVPQVTELAYLLAKDNVPLPRDILTVEEMVDCLCQ
                     LM"
     gene            1812..2675
                     /locus_tag="PK768_06740"
     CDS             1812..2675
                     /locus_tag="PK768_06740"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HQA60705.1"
                     /translation="MSIDVRKLTHIYMPNTPFESVAIKDIDWTINDGEFWGLIGHTGS
                     GKSTLIQHLNGLLKPTSGEIIIDGKNIHGKDVSLKSIRQKVGLVFQYPEHQLFEETVA
                     QDVAFGPKNMGLAEEEVSKRVKEALELVGLSYEEVKDKSPFELSGGQMRRVAIAGVLA
                     MKPEVLVLDEPTAGLDPRGRDEILQEIVDLKQKQNLTVILVSHSMEDIAKIVDKLAVM
                     YKGRMVCQGNPREIFQDYESLVEMGLGIPQVTELMIKLKGRGKDVSSDILTVEEAREE
                     ILKKIRGKGLA"
     gene            2668..3462
                     /locus_tag="PK768_06745"
     CDS             2668..3462
                     /locus_tag="PK768_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQA60706.1"
                     /translation="MREITIGQYIPGNSIVHRLDPRTKIILTLAYMILLFALNNFYGY
                     IFPAAFLILATLLSRIPVRYLVKGLKPLVLIIALTFVLNSFMVKGRVIYELGPLDITY
                     EGLSQGAFMAIRLILLITGTSILTLTTSPIALTDGIERLLSPFRKIGVPAHELAMMMT
                     IALRFIPTLLEETDKIMKAQMARGADFESGNLINRAKNMVPLLVPLFISAFRRADDLA
                     MAMEARCYRGGENRTRLKELKMTSLDIATYAVFVLFAISGIASRFM"
     gene            3477..4208
                     /gene="truA"
                     /locus_tag="PK768_06750"
     CDS             3477..4208
                     /gene="truA"
                     /locus_tag="PK768_06750"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQA60707.1"
                     /translation="MNVKILLEYDGTAYCGWQRQKNALSIQEVLEKAISSVTGENVHV
                     IGSGRTDSGVHALGQVANFKTNTRIPIDKLPYAINSKLPDDIVVKDAEIVPEDFHARF
                     WAKSKIYSYTIYNSKFPSPIYRRYSYFCPHPLDVDAMKRAAQFLVGIHDFKAFMASGS
                     PVKSTVRHVQRLEIIKQGDIVKLEMEANGFLYNMARIIAGTLLDVGTGKRNADEMPSI
                     IESKDRFYAGKTLAAQGLCLVKVNY"
     gene            4290..>4460
                     /locus_tag="PK768_06755"
     CDS             4290..>4460
                     /locus_tag="PK768_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HQA60708.1"
                     /translation="MSSFMAKKEEVKRDWYVIDAAGKPLGRLASRVAMILRGKHKPIY
                     TPHVDTGDYVIIV"
BASE COUNT         1358 a          943 c         1134 g         1025 t
ORIGIN      
        1 aaggatatca ttgccgatgc cgatgtggaa atattaaatc ccgaccttca catagctacc
       61 ctggaaaaag atgcaaaact cttcatggaa ctcacgctgg aaaagggcag gggctatgtc
      121 actgcggaca ggaacaaaaa atccaaccag ccgataggtg ttatagctat tgactctatc
      181 tttactcctg tgcacaaggt aaactttaac gtggaaaata cccgggtagg gcagaggacc
      241 gactatgaca aactgacgat ggagatttgg accaacggcg ccataaagcc ggatgatgca
      301 gtaagtactg ctgccaaaat cctcatagat catttcaatc tgttcttagg tttaaccgac
      361 caggttcaga ccgaagagga acctgttgaa acggaaaaag acgacatgca gaaaatactt
      421 gaaatgccca ttgaagagct ggacctctcg gttcggtcat ataactgctt gaagcgggca
      481 ggtataaaca cgatatatga actaacccaa aaaacggcag aagatatgat gaaagtgcgc
      541 aatctgggta aaaaatccct gcaggaagtc gaatcaaagc ttgccgcttt caatctatca
      601 ctaaaacagc cagatgatta gtaaggaggc gagaacatgc ggaagctggg caggccaagt
      661 ggacatcgaa aaatgatgtt gaaaaacttg acaaccgacc ttttcaggta cggcaggata
      721 gaaaccaccg aaactagggc aaaagaagtc cgtcgcattg ctgaaaagat gataacactg
      781 gccaagaaaa acgatttagc agcaagaagg ttggtattgg ccgagttgct ggatgagact
      841 accgtcaaga atttgtttga taaaatagca cccaaatacc aggaccggaa cggcggttac
      901 gtgagattga caaaaatagg tcttcgccgg ggtgactcta cacctttagc cgtgctggag
      961 cttgtagaat agaaagatgt aaagggggcg gtgaaaataa ccgccgaaat aactgcaaag
     1021 aatgtgtatt accagtattc tgccgatagc agcctggcac tgaataacat aaatttgacg
     1081 attaagccag gcgagtttgt ggccatcata gggccaaacg gttccggaaa gtcgacgctg
     1141 gccaaactct ttaacggcat cttcgtgcct accaagggcg atgtctttgt agatggcctt
     1201 aacaccaagg acaaggataa aatatgggaa ataaggcgaa aagccggtat ggtatttcag
     1261 aatcccgaca accaaatcgt ggccaccgtc gtggaagagg atgtggcctt cggaccggaa
     1321 aacctgggga ttgcgccgga ggaaatacgg cggcgcatcg atgaggccct tagaaccgtt
     1381 gaacttactg aatttgccaa taaggctccc cacctcctgt cagggggtca aaagcagcgg
     1441 gtggcgatag cggggatact ggccatgaaa cccgattgta tcatcctcga tgagcctacc
     1501 gccatgcttg accctgttgg cagaaaggaa gtaatttcga cggtaacaaa actcaatcgg
     1561 caagaaggca aaactgtcat cttaattacc catttcatgc aagaggcggt ttgtgccgac
     1621 agggtcctgg tcatggaaaa aggcaggata gtaatggaag ggaagcccaa cgagattttc
     1681 agccaggttg aaaaactaaa acagtacggc ctggatgtgc ctcaggtcac ggaacttgcc
     1741 tacctgctgg caaaagacaa tgtccccttg ccaagagata tattaactgt tgaggaaatg
     1801 gtggattgtc tatgtcaatt gatgtgagaa agctaaccca tatctacatg ccgaatacgc
     1861 cctttgagtc tgtggcaata aaggacattg attggaccat aaacgacggg gagttttggg
     1921 gacttattgg tcatacaggt tcgggcaagt ctaccctcat tcaacactta aacggacttt
     1981 taaagcccac atccggtgaa atcatcatcg atgggaagaa tatccacggc aaagatgtga
     2041 gcctaaagtc catcaggcaa aaagtcggcc tggtctttca gtaccccgag catcaactgt
     2101 tcgaagagac cgtggcgcag gatgtggcct tcggccccaa gaacatgggc cttgcggaag
     2161 aggaggtttc caaaagggtt aaggaagcct tggagctggt aggcctctca tatgaggaag
     2221 tgaaggataa atcacccttt gagctcagcg gtgggcagat gcgaagggtg gccattgccg
     2281 gggtattggc catgaaacct gaggtgttgg tattagatga gcctacggca ggccttgacc
     2341 ctagaggcag agatgagatt ttacaggaaa ttgttgacct gaaacaaaag cagaatctga
     2401 cggtaatatt agtttcacac agcatggagg atatagccaa gattgtagat aaactggcgg
     2461 tgatgtacaa aggccggatg gtctgccagg gcaatcccag ggaaatcttt caggattatg
     2521 aaagcctcgt ggagatgggc cttggcatac cgcaagttac ggaactaatg ataaaactca
     2581 aaggcagggg caaagatgta tcgtcggata ttttgactgt tgaagaagcc agagaggaaa
     2641 ttttaaaaaa aataaggggg aagggccttg cgtgagataa caatcggaca atacattccg
     2701 ggtaattcca tagtgcatcg cttggaccct aggaccaaga ttattttaac tctggcatac
     2761 atgatacttc tgtttgccct caacaacttt tacggatata ttttccctgc agcttttttg
     2821 atacttgcta cattgctatc caggattccg gttagatact tggtaaaagg gcttaagcca
     2881 ctggtattaa taattgcact cacctttgtg ttaaattcct tcatggtaaa aggtcgggtg
     2941 atttatgagt taggccccct ggatataacc tatgagggtc tttcacaggg tgcttttatg
     3001 gccatacgcc tgatcctgtt gattacgggc acatccattt taaccttgac aacgtcaccc
     3061 atagcgctga ccgacggcat agaaaggcta ctttcgccct ttaggaaaat tggtgtgccg
     3121 gcacatgaac tggccatgat gatgacaata gccttgaggt ttatccccac attgctggag
     3181 gagaccgata agatcatgaa ggcccagatg gcaagagggg cggactttga aagcggcaac
     3241 ttgataaatc gggccaaaaa catggttccc ctgctagttc cccttttcat cagtgccttc
     3301 agaagggcgg atgatttggc aatggccatg gaagccaggt gttaccgagg cggcgagaac
     3361 aggaccaggc tgaaggaatt aaaaatgaca tctcttgaca tagcaacata tgcggttttt
     3421 gtattatttg ccatcagtgg cattgcaagc cgttttatgt agcaggtgta tgctgtatga
     3481 acgtaaaaat actgctggaa tatgacggaa cagcatattg cggttggcag aggcagaaaa
     3541 acgccctatc catccaggaa gtcctggaaa aggcgatcag ctctgtaacc ggtgaaaatg
     3601 ttcatgtaat aggctccggc cgcacagaca gcggagttca tgcactaggc caggtagcta
     3661 attttaagac aaatacccgc attcccatag acaaactgcc ctacgccatc aacagcaagc
     3721 tgccggatga catagttgta aaagacgccg agattgtgcc cgaggacttt cacgctcggt
     3781 tttgggcaaa atccaagatt tacagctata ccatttacaa ttcaaagttt ccatcgccca
     3841 tttacaggag atacagctac ttctgccccc atccgttaga tgttgatgcc atgaaaagag
     3901 ctgcccaatt cctcgtgggc atccatgact ttaaagcatt tatggcatcg gggagcccgg
     3961 tgaaatccac cgtgcgccat gtgcagaggc tggaaattat aaaacagggc gatatagtga
     4021 aacttgagat ggaagccaat ggctttttat acaacatggc aaggataata gccggaacct
     4081 tgctggatgt gggtacgggc aaaaggaatg ctgatgaaat gccttccatc atcgaatcaa
     4141 aggacaggtt ttacgcaggg aaaactcttg cagcccaagg cttatgcctt gtaaaggtta
     4201 attattgaca tctccgcata aaataacagt gcttgacaca caaaaaggcc tattataaaa
     4261 taaccttgtg caaaaaagga gggaaaacaa tgagttcatt catggcaaag aaggaagaag
     4321 taaaaagaga ctggtacgta atcgatgctg caggcaaacc tttgggcagg cttgcgtcaa
     4381 gagtggccat gattttaagg ggcaaacaca agcccatata tacacctcat gttgatacag
     4441 gggattacgt aattatcgtt
//
LOCUS       DAONUO010000167         4443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14014_length_4443_cov_3.346171, whole genome shotgun sequence.
ACCESSION   DAONUO010000167 DAONUO010000000
VERSION     DAONUO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14014_length_4443_cov_3.346171"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PK768_06760"
     CDS             <1..348
                     /locus_tag="PK768_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HQA60709.1"
                     /translation="MKGSDLIQALEHGVSFYPEISGAFPQVAGIDFSFSADKEPGNRL
                     AEVTIGGQELDPEKMYRIAINDFMLAGGSGYTIFEKGRVLGEYGLLVDIVAEYIQNNL
                     NINVESLVSLAAF"
     gene            424..672
                     /locus_tag="PK768_06765"
     CDS             424..672
                     /locus_tag="PK768_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HQA60710.1"
                     /translation="MTQSLDSGRGPENKLMEEIEKALLEIELSEKAFQWAQNDADEVD
                     LAIARKQAAIRHFDFLIKQAKKAGLKLDRKQLIEKILK"
     gene            complement(763..2091)
                     /locus_tag="PK768_06770"
     CDS             complement(763..2091)
                     /locus_tag="PK768_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60711.1"
                     /translation="MFKSLKVLPLLTRLLFAARMAAHGILAHPLRSALTILGVAIGVT
                     SVVSLMGIGEGARQAVVAQFESLGANVIVMKANDPSVEFDPDEVPDLVERVDGMDMAT
                     PVVHTNAVMRWRRARGTVDIVGVNSKFPEIRDNELIAGHFFTELHVSQRSPVVVLGYN
                     MGTGLMGGRNPVGQVISLNGKTFRIIGVLAPKGRGKADDIDDKIVIPYTTALRIAEKK
                     TVPEIWGKAPTPSKADLAVVQLGRIFKRKLGLDQSAPTTMPGDQDQMSGDGVFFEAVE
                     AVPIGPAGTNENNPIIPGGEDLITITNLNQLVQEADQANRVMTLLLGGIAAVSLLVGG
                     LGIMNIMLVAVTERTQEIGVRRALGAKQTDLLIQFLLEALYVSGIGAIAGTAGGIWGL
                     NAFARHGFETAISFHAIKIAVVVALTSGLLFGIYPAISASSVPPIEALRR"
     gene            complement(2120..3799)
                     /locus_tag="PK768_06775"
     CDS             complement(2120..3799)
                     /locus_tag="PK768_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQA60712.1"
                     /translation="MAQRILMIALIIVIVIGGGFYAYRELVPPPAEEAQGPVYSTKPV
                     TRGDISVGVEVSGPLNPSSSGSILAPGGRNYYEISSSNIQYTLVEILAEEGEAVEMGQ
                     VIARLAAPQLQNQIKNVEAELDNEKRFLSELTGVPEDRLYVINPAQGVVLRAPIDGRV
                     SGLSVSEGKEIKQGQIVARIVDDSHFKINAKVNQNEFPLINLGQKLAIEFTFFEGIYF
                     GEVVDINPNPVPDSDEDSPTRNFVYWITVEGKNPGLVQPGLQVRLGIADSTTNPTRVS
                     WFASPATVEGFVNEERVLSTVEALATQVHVREMEMVKKGDPIVSLSGSDVQKTIEEKL
                     NKIREKETELNDLYSKAGQMEVRAPMDGIIARWDIQLGAQISPGDWMGYVFNTSDMNL
                     WVEVDDIDVLMIKHGSPATVTVEALPGETFEGVVEHVATMGKDMQGITRFGVDIRVKG
                     SSELRPGMQASAYIDAGSAKDVLLIPVEAIFEEDGVQKVEVLNPDGTTKAVAVKLGLM
                     NDRFAEVKSGLDEGELVITGSSADLLPSQHIGRKESILPSNNSDDDDNKVQ"
     gene            complement(3839..>4443)
                     /locus_tag="PK768_06780"
     CDS             complement(3839..>4443)
                     /locus_tag="PK768_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60713.1"
                     /translation="SIEEGEFISIMGPSGSGKSTMLNIIGCLDKPSSGTYEIAGKRVE
                     KLGDGQLAEIRNEFIGFVFQSFHLLPDLTALENVELPLIYRGLIGKERRKKAVEALKS
                     LGLGERIYHRPSQLSGGEQQRVAIARAIVGDPKVILADEPTGALDSKTSETIMEIFQE
                     LNNERGITIVQVTHEKSIAEYSKRIYHLLDGKIERIEDLA"
BASE COUNT         1175 a         1076 c          917 g         1275 t
ORIGIN      
        1 atgaagggct ctgacctcat tcaagccctt gagcacgggg tctccttcta tccggaaata
       61 agcggagcgt ttccccaagt ggcgggaatc gattttagct tttctgccga caaggagcca
      121 ggaaatagat tagctgaagt gacaattgga ggtcaagagc ttgacccgga aaagatgtac
      181 agaattgcaa taaacgattt tatgcttgca ggaggcagcg gctacaccat ttttgaaaag
      241 ggcagggttc taggagaata tggattgtta gtcgatattg ttgccgaata cattcaaaat
      301 aatcttaata taaatgtgga aagccttgtt tcattagcag ctttttaatt ttgttataga
      361 tgttgcaagt aattcctctt tcctgcatag gatatactac aatcttatca ggagggggtt
      421 gtcatgaccc aaagtcttga tagcggtaga ggacctgaga acaaattaat ggaggaaatt
      481 gaaaaagccc tcttggaaat cgaattatca gaaaaggcat ttcaatgggc ccaaaatgat
      541 gctgatgaag tggatttagc aatagcaaga aaacaagcgg ccatccggca ctttgatttt
      601 ttgataaaac aagctaaaaa agcaggtctt aaacttgata gaaaacagtt gattgaaaaa
      661 atattgaaat agtttgttaa gtcacaatga atatcaaaaa agggccaggt ttgaagggaa
      721 caagcctgac ccctttggtt tcataaactt tctgtggcat cgttagcgcc tcaaagcttc
      781 tattggagga actgaagatg ctgaaatagc cgggtatatt ccaaagagaa ggccggaagt
      841 cagtgccaca acaactgcta tttttatagc gtgaaaactt atagcagttt caaatccatg
      901 gcgggcaaag gcgttcaagc cccaaattcc accggcagtg ccggcaatgg caccgatacc
      961 gctgacatac agagcttcga gcaaaaactg tataagcaaa tcagtttgct ttgccccaag
     1021 ggcacggcgt actcctattt cctgcgtgcg ttccgttacc gccacaagca taatattcat
     1081 tattcctaga ccgccaacta atagtgatac tgcagcaata ccaccgagca atagggtcat
     1141 tacccggtta gcttggtcgg cttcctggac caactggtta agattggtta tggttattaa
     1201 gtcctcacca ccgggaataa tagggttgtt ttcattagtt cctgcgggac ctattggtac
     1261 agcttctaca gcttcaaaaa ataccccatc ccccgacatc tggtcttgat ctcccggcat
     1321 ggtagtaggt gcactttggt caaggcccaa ctttctctta aaaattcttc caagttggac
     1381 aactgccaaa tcagcctttg atggagttgg ggcctttccc catatttcgg gcacggtctt
     1441 tttctcggca attcttaagg cagtagtgta gggtataact attttatcat cgatgtcatc
     1501 tgctttgcct cgacccttag gcgcaaggac tcctattatc ctaaaagtct tgccattgag
     1561 actaataacc tggcctactg gatttcttcc tcccatcaag ccagttccca tattgtaccc
     1621 caaaacgacc acgggcgacc tttgagatac gtgcaattcg gtaaaaaagt ggccagctat
     1681 taattcatta tcccttattt ccggaaattt gctgttcact ccaactatgt ccacggtccc
     1741 ccttgccctt cgccaacgca tgaccgcatt tgtatgaaca acgggtgtag ccatatccat
     1801 accgtctacc cgctctacca aatccggcac ctcgtcgggg tcaaactcta cagaagggtc
     1861 gttagccttc attacaatta catttgcacc taaactttca aattgggcta caacagcctg
     1921 ccttgcacct tcgcctattc ccattagact gacaacggat gtgacaccga tagccacgcc
     1981 caagatggtt agtgcagaac gcaagggatg tgcaagtata ccgtgggctg ccattctggc
     2041 tgcaaacaac aatcgcgtaa gcagtggcaa tactttaagt gatttaaaca ttttttcacc
     2101 cctcaattgg ttgccggcct tattgcactt tgttgtcgtc atcgtctgaa ttgttggaag
     2161 gcaaaatcga ttccttcctt ccgatgtgct ggcttggaag caaatcggcg ctgctgccag
     2221 ttatgaccag ctcgccctca tctaggccac ttttaacttc tgcaaatcta tcgttcatta
     2281 ggccaagttt tacggcaacc gcctttgtag taccgtcggg atttagcact tcaacttttt
     2341 gcacaccatc ctcctcaaag atggcctcta caggaatcaa caggacatct tttgcgcttc
     2401 ctgcatcaat gtaagcgctt gcctgcatgc ctggtctgag ctcggaactg ccttttacgc
     2461 gaatatcaac accaaaacgg gtaattccct gcatatcttt gcccatagtg gcaacgtgtt
     2521 ccaccactcc ctcaaaagtt tcacccggaa gagcctccac agtaacggtg gcaggggagc
     2581 cgtgttttat catcagcaca tcgatgtcgt caacttctac ccacaaattc atatcacttg
     2641 tattaaaaac atatcccatc cagtctcccg gactgatttg agccccaagc tgaatgtccc
     2701 atctggcaat tattccgtcc ataggtgccc tgacttccat ttgaccagct tttgagtaaa
     2761 gatcgtttag ttcggtctct ttttcgcgta tcttgttgag cttttcttca atggtctttt
     2821 gaacatctga accggatagg gagacaatcg ggtcaccttt ttttaccatt tccatttccc
     2881 taacatgcac ctgggtagcc aatgcttcaa cggtagataa aactctctct tcattgacaa
     2941 aaccttcaac agttgcaggt gaagcaaacc aggaaactct tgtaggattg gttgtggaat
     3001 cggctattcc aaggcgaact tgaagacccg gctggaccaa accggggttt tttccttcta
     3061 ctgtgatcca atatacaaag tttctggtgg ggctgtcttc gtcgctgtct gggacaggat
     3121 ttggattaat atccaccact tcaccaaaat atatgccttc aaaaaatgta aactctatag
     3181 ccaatttttg tccaagattt attaaaggaa attcattttg attaaccttt gcattaattt
     3241 taaagtggga atcatctact attcgtgcaa caatttgacc ttgtttaatt tccttgcctt
     3301 ctgaaaccga taaacccgaa accctgccat ctataggtgc cctaaggaca acaccttggg
     3361 cgggattgat tacgtacagg cggtcttcgg gcacacccgt taactcggat aaaaatcttt
     3421 tttcattatc cagctcagct tccacatttt taatctgatt ttgcagttga ggagctgcga
     3481 gccttgcgat cacttgaccc atttcaactg cttcaccttc ttcagctaaa atctctacta
     3541 gagtgtattg aatgttgctg gaagatattt cataataatt tctgccgccg ggggccagta
     3601 tactgccgct gctggagggg tttaaaggac cggagacttc aacgccgacg ctaatgtctc
     3661 ctcttgtcac gggttttgta gaataaacgg gaccttgcgc ctcctcagca gggggcggta
     3721 cgagttccct ataggcatag aatcccccgc caattacaat tactataatt aatgcaatca
     3781 tcaatattcg ctgtgccatt acactacctc caagctataa aattaaaaaa tgaatttttc
     3841 atgctaaatc ctcaatccgc tctatttttc cgtctaacaa gtggtatatc cttttactat
     3901 actcggctat gcttttttca tgggtaacct ggactatggt tatgccccgc tcattgttta
     3961 actcttgaaa tatctccatt atcgtttcac tggttttcga atcgagggca ccggttggtt
     4021 catcagcaag tattaccttg ggatcaccta ctattgcccg cgcaattgcc acccgctgct
     4081 gctcgccgcc tgaaagttga gaaggtcggt ggtagattcg ctctccaagg cccaatgact
     4141 tcagtgcttc cacagccttc ttgcggcgct ctttgcctat taaaccgcgg taaataaggg
     4201 gcagttccac attttccagg gcggttaaat ccggaagcag atgaaaactt tgaaacacga
     4261 accctataaa ctcgtttctt atttcagcca gctgaccgtc tcccaatttt tcaacgcgtt
     4321 tgcctgcaat ttcgtatgta cccgatgaag gtttatcgag gcaaccgata atgttgagca
     4381 tggttgactt acccgagccg gaaggtccca ttattgaaat aaattcgcct tcttcaatgc
     4441 tca
//
LOCUS       DAONUO010000168         4422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14077_length_4422_cov_4.109228, whole genome shotgun sequence.
ACCESSION   DAONUO010000168 DAONUO010000000
VERSION     DAONUO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14077_length_4422_cov_4.109228"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..156
                     /locus_tag="PK768_06785"
     tRNA            80..156
                     /locus_tag="PK768_06785"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:114..116,aa:Met,seq:cat)
     gene            162..236
                     /locus_tag="PK768_06790"
     tRNA            162..236
                     /locus_tag="PK768_06790"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:195..197,aa:Glu,seq:ttc)
     gene            438..2810
                     /gene="spoIIE"
                     /locus_tag="PK768_06795"
     CDS             438..2810
                     /gene="spoIIE"
                     /locus_tag="PK768_06795"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HQA60714.1"
                     /translation="MLDRAHLNLPDIFAGKSSEVKSISTLSLPLGSASREFIQLSIFA
                     VLFGMASINGLHPFALSFAAANLIYRESFIVPALFSFLGSLIDMKSAASIRYLAAMAI
                     FFFTYSLLRKANRQNRLLLLGLGVFFSNMIPGLIYLKAHGISPYDLFLLLAESGLAYI
                     MTFIIPGGMPWLFKEPIQPTERNICMAVIAGVILSIARKIVWLGVNLRDVLGVFAVLL
                     MALVDGPGAGAATGIIIGVTGFSFSLSPWSTAVMAFSGLVSGSFNKLGKMGVIAGFSL
                     GYLLYNFYVNSMGEAIISLPVLAAAFILVLLLPQKLISRMRLYFSSSHNITNLNSLKL
                     EERARDRLYELASVLNDLGRAFKDALIKKEERILSAEYFDLVCRRVQLEVCSDCGMRR
                     ICWEKELKRTMASFYTLIKQQEGLVDRKAIPFLFKSRCGRAENIKRIVKDQSDLFKVK
                     HQMDRIMKANQELAQLHFAKAAEMIKALAEDTCDEEYERNMGTELAEKLSQLGVSVER
                     IYTDYSDGRIVANIVKSPCTNNKHCDLLIPLAASDVLGRKISTKIIDCPLKSGSSNCR
                     LKVVTKGPLQVSVGVAGVAKEGQTVSGDGFTFMELENGRFLLALCDGMGVGKAAAKHS
                     EKTLAILERLLEAGFSQETAVKVTNAAMIATNPDESFSTIDAALIDTAAGKIRFIKAG
                     APTSFIKHRSKIEIIRGGSLPVGIMDEVVPKVTEKTIGPGDMVIMVTDGVIDALSDKQ
                     NGEEVIRKILSEMKTTNPQDIAEGLLKKAKGQKSIRDDMTVLVGSIWEKR"
     gene            2957..4348
                     /gene="tilS"
                     /locus_tag="PK768_06800"
     CDS             2957..4348
                     /gene="tilS"
                     /locus_tag="PK768_06800"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQA60715.1"
                     /translation="MNVEKQFLETIEKYNMIKKGDKIVLGVSGGPDSVCLLHLFDKYR
                     HQLGVSLYAAHLDHSFRGKESEEDAHFVKALCEKRDIPFFGEQFDVPSFAKRYSLSPE
                     DAARRVRHDLFRRVKEQVKAHKIATGHNQNDQEETILMNILRGAGLDGLVGIDAVSGC
                     YIRPLIEIPRNEIEGYLKQKGIPSRIDSTNLSSEYFRNSIRLELIPLIQEKYCPHLGQ
                     SLRRLSEIAKRDLSFLNQATDKAERDSVKYESDKVSINVESFSKQHAAIKYRLARKAI
                     ERLAGNLKDFEIRHTGLLVDFIEKAQPGSMLDLPKNLKGIKEYDELVISKAGFDEVPD
                     YYYTLPVPGELTVKEAGIIIKAYIKPKVEVAITPDANTAYLDYDKTGGNLTIRNRRPG
                     DRFKPLGGTGLKKLKDFFIDEKLPRLKRNRIPIVEADGRIVWVGGMRIDDRFKVTDET
                     KTVLVLTVGALRE"
BASE COUNT         1297 a          894 c         1086 g         1145 t
ORIGIN      
        1 ggtagctcgt cgggctcata acccggaggt cgtcggttca aatccggccc ccgccaccaa
       61 tataaaacca tatatacaag gcggcgtagc tcaggtggct agagcatgcg gttcataccc
      121 gcagtgtccg gggttcaaat ccctgcgccg ctaccaaaca tggccctgtg gtcaagcggt
      181 taagacaccg gcctttcacg ccggtaacag gggttcgatt cccctcaggg tcaccataaa
      241 tgtgaacaag tcgctattat agcggcttat ttttttatct tctagtattg tttatcttct
      301 aatattgtcg ccaatggttt tttccggttc atttttgtcg cacaattttt tgtgtgcctc
      361 aagctatatt gacaatcttt acaggccacc ttcagtataa tggtatctgc caataatctt
      421 caggaaggtg gacaatcttg cttgaccgag ctcatcttaa tttgccggac atttttgcag
      481 ggaaatcaag tgaagttaaa agtatttcta cgttatccct acccttggga agtgcaagca
      541 gggaatttat tcagttgagc atttttgcag tgcttttcgg gatggcttca ataaatggcc
      601 tacatccttt tgccctctct tttgctgcag ctaatctcat ttatagggaa agctttattg
      661 tcccggcgct tttttctttt ttaggaagct taatagatat gaaaagcgca gcatctatta
      721 gatatttagc tgccatggct atattctttt ttacttactc cctgcttagg aaagcgaatc
      781 gtcaaaatcg gcttttgctg ctgggtctcg gagtgttttt ttcaaatatg attccgggcc
      841 ttatctatct gaaagctcac ggcatttctc cctatgatct attcctgctt ttggccgaat
      901 ccggcttggc ttatatcatg acattcatta ttccgggagg tatgccctgg ctttttaaag
      961 agcccataca gcctacagaa agaaatattt gcatggcagt tattgcaggg gtcatcctgt
     1021 caattgcacg aaagattgtg tggcttggag taaacctcag ggatgtgctt ggggtctttg
     1081 ccgttttact tatggcgtta gtagatggtc ccggtgcagg ggctgcaacc gggattatta
     1141 taggggtaac gggcttttct ttcagccttt caccctggtc aacagcagta atggcttttt
     1201 ccggcctggt atcgggctcc tttaacaagc tagggaaaat gggtgtaata gccggatttt
     1261 cccttggcta tctgctgtac aatttttacg taaattccat gggcgaggca ataatttcac
     1321 tgccggtttt agctgcagct tttatcttgg tcttgttact gccgcagaag cttataagca
     1381 ggatgcgcct gtactttagc agttcgcata atatcacgaa tctcaacagc cttaagctgg
     1441 aagaacgggc aagggacagg ctctatgaat tagcctcggt tctcaatgac ctgggacggg
     1501 catttaaaga tgcgttgata aaaaaggagg agcggatact ttctgcagaa tacttcgatt
     1561 tggtctgccg aagggtgcag cttgaggtct gctctgattg cgggatgcga agaatttgct
     1621 gggaaaaaga actaaagaga acaatggcat ctttttatac cctgataaaa cagcaggagg
     1681 gacttgttga tagaaaggcg attccctttt tgtttaaatc gcgatgcggt agggcggaaa
     1741 acataaaaag aatcgtgaag gaccaaagtg acctatttaa agtaaagcac cagatggata
     1801 gaataatgaa agcaaaccag gaacttgccc aattgcattt tgcaaaagca gccgaaatga
     1861 ttaaagcact ggctgaagat acctgtgacg aagaatacga gcgaaatatg ggcacagagc
     1921 tggctgaaaa gctttcacag ctgggagtga gtgttgaaag gatatatacc gattacagcg
     1981 acggcagaat tgttgcgaat atagtaaaat caccctgcac aaataataag cactgtgacc
     2041 tattgattcc cctagcagca tcggatgtac tgggtaggaa gattagcacc aaaataattg
     2101 attgtccgct aaagagcgga agttccaatt gtcgattaaa agtcgtgaca aagggccccc
     2161 ttcaggtgtc agtaggggta gcgggagtgg ccaaagaagg gcagactgtc tccggagacg
     2221 gctttacatt tatggaattg gaaaacggga ggttcttgct ggccctatgt gacggcatgg
     2281 gagttgggaa ggctgctgcc aaacacagcg aaaagacact ggccatactt gaaaggctgc
     2341 tagaagcggg cttcagtcag gaaacggccg taaaggtgac taatgcagct atgattgcca
     2401 caaacccaga tgagagcttc tctacaatag atgctgccct catagatacc gcagccggga
     2461 aaataagatt tatcaaggcc ggtgcaccga ctagcttcat aaagcaccgc agcaaaatag
     2521 aaatcattcg tggtggaagt ctgccggtag gtatcatgga tgaagttgtg cccaaggtaa
     2581 ccgagaaaac cattggccca ggtgacatgg tcataatggt aaccgacgga gttatagatg
     2641 ccctttcaga taagcaaaac ggcgaggaag tgatcaggaa aatattatct gaaatgaaaa
     2701 ctactaatcc ccaggacatc gccgaagggc tcctaaaaaa ggcaaagggg caaaaaagca
     2761 taagagatga catgaccgtc ctcgtgggca gcatatggga gaaaaggtag gggcgtgggg
     2821 atcaccggac ttccggggtg gctaaagtgt tcacagcctg tatgagatga tacaattgat
     2881 gttttcccat ataaaggata taatgtaagc gagaaatatg agaacaaatg tgggcagata
     2941 ggaagaaacg ataacaatga atgtggagaa acaattttta gagaccatag aaaaatacaa
     3001 catgattaaa aagggcgata agatcgtcct tggagtttct ggtggacctg actccgtttg
     3061 tctgctccac ctttttgata aatatagaca tcaactggga gtttccctat acgcggcaca
     3121 ccttgaccac agttttaggg gaaaagaatc agaggaagat gcccattttg tcaaggcttt
     3181 gtgtgaaaaa cgggatattc cttttttcgg tgaacaattt gatgtacctt cttttgctaa
     3241 aagatacagt ctttctcctg aagatgcggc ccggcgagtg aggcacgatt tgttccgtcg
     3301 cgttaaagaa caggtcaagg ctcacaagat agcgaccggc cacaaccaaa acgaccagga
     3361 agagaccata ctcatgaata tattaagagg tgcgggccta gatggccttg tgggaatcga
     3421 tgcagtttcc ggctgctaca tcaggcccct gattgagata ccgagaaatg agattgaagg
     3481 gtacctaaag cagaagggca tcccttccag gatagattct acaaacctca gcagcgaata
     3541 ctttagaaac agtattcgat tagaactcat tccccttatt caagaaaaat actgcccgca
     3601 cctgggacaa tccctcagac ggttatccga gatcgcaaag cgggacctgt catttttaaa
     3661 tcaagcgact gacaaagctg aaagagattc ggtaaagtat gagtcagaca aagtctctat
     3721 taatgtagaa agtttttcaa agcagcatgc ggcaataaaa taccggctgg caagaaaggc
     3781 aattgaaaga cttgccggga atctaaagga ttttgaaata agacatacag ggctgctggt
     3841 agattttatt gaaaaagccc agccgggcag tatgctcgac ctgcccaaaa accttaaagg
     3901 cataaaagaa tatgatgagc ttgtcatctc aaaagcaggt tttgatgagg ttcctgacta
     3961 ctactatact ttaccggttc ccggggagct aacagtaaaa gaggcgggca taattatcaa
     4021 agcatacatt aaaccgaagg ttgaagttgc aataactccg gatgccaata ctgcctactt
     4081 agattacgat aagaccggcg gcaacctgac tataagaaac cgccggcctg gagatagatt
     4141 caaaccctta ggaggaacag gattaaaaaa gctcaaggac ttttttatcg acgaaaagct
     4201 tcccaggcta aagagaaatc ggatacccat tgtggaagca gatggcagga tagtatgggt
     4261 cggaggcatg aggattgacg atagattcaa ggtaaccgat gaaacgaaga cagtgttggt
     4321 cttgactgta ggagcattac gagaataaca tgagaatttt atatactcgt ttttattatc
     4381 atgggacaag tacaggaatg cagcattcct gtattttttt at
//
LOCUS       DAONUO010000169         4400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14162_length_4400_cov_3.725892, whole genome shotgun sequence.
ACCESSION   DAONUO010000169 DAONUO010000000
VERSION     DAONUO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14162_length_4400_cov_3.725892"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..1209
                     /gene="guaA"
                     /locus_tag="PK768_06805"
                     /pseudo
     CDS             250..1209
                     /gene="guaA"
                     /locus_tag="PK768_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002009981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     gene            1420..2508
                     /gene="fbp"
                     /locus_tag="PK768_06810"
     CDS             1420..2508
                     /gene="fbp"
                     /locus_tag="PK768_06810"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HQA60716.1"
                     /translation="MSSRVTLSVFKADVGGYVGHGSVHPDMLHKAEETLKEAGKHLLI
                     DFFVTNVGDDINLVLTHREGVDAEAIHALAWNTFLNCTEVAKSLKMYGAGQDLLSDAF
                     SGNIKGMGPGIAEMEFEERPSEPIIVFMADKTEPGAWNFPLYKIFADPFNTIGLVIDP
                     KMHSGFCFEVLDLFEDKSVTFSCPEELYDLLVFLGAPNRFVVKSVFTKNNEIAAVSST
                     QKLNQMAGRYVGKDDPVLIVRCQSGLPAVGEVLEPFANPHLVSGWMRGSHHGPIIPVS
                     QKQAIPTRFDGPPRVIAMGFQLSSGRLIGPQDLFADVAFDRAREKALEIADYMRQLGP
                     FEPHRLPLDEMEYTTMPQVMERLKDRFK"
     gene            2744..4330
                     /locus_tag="PK768_06815"
     CDS             2744..4330
                     /locus_tag="PK768_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQA60717.1"
                     /translation="MQKRVKEGASKVDYRLLYKKQILDYLESLKSKEQSIWQMDEAMR
                     RLFRISDQVVPVCLSKLRENDEQLAPVICYALEYANDFSVVEPLMDILIMQEVSDKIK
                     ARILTVLTHYGIDAGELPLDMIMNDFDKMASDSMEEMLADIYRDPFLIPYLLDDLEEF
                     NLEMKLAYIMDLAALKDERAILFLEILASIDEPPVAAEAIKALGQISSGKALYVLEKL
                     KSRVSDENNKKLIHRESQRLKFKGVTMEVFKPWEKLKQPTKIYISSIDGLGSRVLWIA
                     WKNPFKNRKLCFMNLLIGVDAGIKDCWGASNITAREFNSSIRDFSKTMFVTKCDLEYA
                     ILLINDALFVNKTNTSPTPYQFYFWKNLLEQHSVIKQQQYEPSFADYDLDMIKKDLEC
                     LKNTFDLYDSGFFNDWFIAHPRVYDFAEENKSKRAYSIKKMTYQKMENLFAKFSEELI
                     EPNEDKIRRMLLLSADLLDKVDEKSLVKTVLSAYYNMDIKPLYYHPFIQRMVIESIKV
                     ALSNMKNGFDMRQNFRDFDQ"
BASE COUNT         1435 a          787 c          965 g         1213 t
ORIGIN      
        1 ttaacgacaa tttttggcta aagttaaaaa ttgatgaaaa aaaattgccg tagcttttga
       61 aatgcagatg atagaacaga ttccatatga aggccatgat gtaaggatgg acaagataat
      121 tactgaaaag ggaatgtacg gtccattttg attggccgca tgcccatatg aatacgctat
      181 acccttttgc ctttattcat cttgtaatta ttatatatca atttttcggg aggaatcaat
      241 atgaacaaaa gcaggggaac ttggtccaca aaggctttta tagaggagca gattaaactc
      301 ataaaagaca aggtgggaga taaaaaggcc atatgtgcct taagcggtgg tgtggactca
      361 tcggtagccg cccttttagt gcacaaggcc ataggcgata acttaacctg tatctttgtg
      421 aacaacggcc tgctacgcaa aaacgaacct gaatccgtca tccaaacctt taaagataag
      481 ctaaatatga acctgatagc tgtagatgct tcggaacgct tcttaaataa actaaaaggc
      541 gtaattgatc ctgaaaaaaa gcgcaagatt ataggagaag agtttatcag ggtttttgaa
      601 gaagaagcgg caaaacttgg cgaagttgac tttctggtgc agggcacagt atatcccgat
      661 gtaattgaaa gcggcagttc atcagcggga gttgtcaaga gccatcacaa tgttggcgga
      721 ctaccggagg acttggagtt tgaactaatc gagcccctgc gggaattgta taaggacgag
      781 gtgcgaaaag tcggtctgga attaggccta tctgaagaac tcgtgtatcg ccagcctttc
      841 cccggccccg gtcttgctgt gcgtgtttta ggcgaagtta caagggaaaa acttgagatt
      901 ttaagggaag cagatttcat agttacagat gaaataaaaa aagctggaat ctataggaat
      961 ctttggcagg cctttgccat tctacccgga atacgaagtg taggagtaat atgggatagg
     1021 aggacctata cgcatacagt agctatcagg gcaattgtaa gcgataacgg catgacaggc
     1081 gattgggcaa ggctacctta tgatttgttg gatactattt caactaggat tacaggtgag
     1141 gtaaaaggag taaaccgcgt tgtatatgac atcacatcca aaccaccggc gactatcgag
     1201 tgggaataaa taattaagat tcgaagagta taacatgcca gttaaaaggg ctgttatact
     1261 ctttttttgc atgtaaaatg tcatactatt taatatatta ggactatcat ttgaagcagg
     1321 aatgtttaaa gatgtagcga atatatatat agtgtagcac aattaaactc ttttaaaaga
     1381 tattgtacaa cataataata ttgtcaggag gcatgcgaga tgagttcaag ggtaaccctt
     1441 tcagtgttta aagcagatgt tggcggatat gtaggtcacg gaagcgttca tcccgatatg
     1501 ctgcataaag cagaggagac actgaaagaa gcgggaaagc atcttttgat cgactttttt
     1561 gtaactaatg taggtgacga tatcaacctg gttctcaccc acagagaagg agtagatgcc
     1621 gaagcaatac atgccctagc ctggaatacc tttttaaact gtaccgaagt tgccaaaagt
     1681 ttaaagatgt atggcgccgg gcaggacctt ttgtcagacg ctttttcagg caacataaag
     1741 ggcatggggc caggaatagc cgagatggag tttgaagaac ggcctagtga gccaataata
     1801 gtatttatgg cagataaaac cgagccgggt gcctggaatt ttccacttta taaaatattt
     1861 gctgacccct ttaataccat cggccttgta atagacccta agatgcattc aggattttgc
     1921 tttgaagttt tggacctttt tgaagataag agcgttactt tttcatgtcc tgaagagctg
     1981 tacgatttgc tggtattttt aggagcccca aatcgctttg tagtaaaaag cgtgttcaca
     2041 aaaaacaatg agatagctgc cgtttcttca acgcaaaagt taaaccaaat ggcagggcgc
     2101 tatgtgggca aggatgatcc cgtcttgata gtaagatgtc aaagcggcct tccggcagtg
     2161 ggagaagtcc tggagccctt tgccaatcca catcttgtat caggctggat gaggggttcc
     2221 catcatggcc cgatcattcc cgtatcgcag aagcaagcta ttcctacccg atttgatggt
     2281 ccgccccgag taattgccat gggctttcaa ctgtcaagtg ggagacttat aggtccgcag
     2341 gatctttttg ccgatgtggc tttcgatagg gcacgcgaaa aagcccttga aattgctgac
     2401 tatatgaggc agctagggcc ctttgaaccg caccgcttac ccctagatga aatggaatac
     2461 actacgatgc cgcaggtaat ggagagatta aaagatcgtt ttaaataatg acctaaaata
     2521 ccgggtgaca tgaaaggaaa ttccgacaat gtcacccttt ttttattgga aaatgacaaa
     2581 attcatcgtg tctttattta aattcccatt aaatacaatt ttatgataat atatatagta
     2641 acaatatatg ataaagttca ataacggtag attacattgc taaaaagcat gtattatatt
     2701 ataatataga agcgattttt tgttatcttg aaaggagcgg gcaatgcaaa agagagttaa
     2761 ggaaggagct tctaaagtcg attaccgact tttatataaa aaacaaattt tagattacct
     2821 ggagtcctta aaaagcaagg aacagtcaat atggcaaatg gacgaagcca tgagaagact
     2881 ttttcgcatc agtgatcagg ttgtcccggt ttgcctgtca aaacttaggg aaaatgacga
     2941 acagctggca cctgtaatat gctatgcctt agaatatgcc aatgatttta gtgtagttga
     3001 gcccttaatg gatatactaa taatgcagga agtttccgat aagataaaag ctcgcatact
     3061 gaccgtgtta acccattacg gcatcgatgc gggagagctt cccctagaca tgataatgaa
     3121 tgattttgat aaaatggctt ccgattcaat ggaggagatg cttgctgaca tctaccggga
     3181 ccctttttta attccgtatt tactggatga tctagaagaa ttcaaccttg aaatgaagct
     3241 ggcttacata atggacctgg ctgcactaaa agatgaaaga gctattttat ttttagaaat
     3301 attagcatct atcgatgagc cccctgtcgc agctgaagcc ataaaagccc tcggacaaat
     3361 atcatcaggc aaggcccttt atgttctgga aaaattaaaa agcagagttt cagatgagaa
     3421 taacaaaaaa ctcattcatc gcgaatctca acgactaaaa tttaagggcg tcactatgga
     3481 agtttttaag ccttgggaaa agcttaaaca gcccactaag atttatattt cttccataga
     3541 tggtcttggc agtcgggttc tttggatagc ctggaagaat ccctttaaaa accgaaaact
     3601 gtgttttatg aacttgctca taggtgtaga tgcgggaatt aaagactgct ggggagcatc
     3661 caatataact gctagggaat tcaactcttc cattagagat ttttcaaaga ccatgtttgt
     3721 cactaaatgc gatttagagt atgctatttt actgataaac gatgctctat ttgtaaataa
     3781 aacaaataca agtcccactc cctatcagtt ttatttttgg aaaaatctat tagaacaaca
     3841 cagtgtgata aaacagcaac aatatgagcc gagttttgcc gactatgatt tagacatgat
     3901 aaaaaaagac ctagagtgct tgaaaaacac ctttgattta tatgacagcg gtttttttaa
     3961 cgattggttt atagctcatc cacgtgtcta cgattttgcc gaagaaaata agtcaaaaag
     4021 agcttattcc attaaaaaaa tgacctatca aaaaatggaa aacctgtttg ccaagttcag
     4081 cgaagagctc atcgaaccaa atgaggacaa aataaggcgt atgctgttgc tttcagctga
     4141 tttattggat aaagttgatg aaaaaagcct agtaaaaaca gtcctgagtg cctattataa
     4201 tatggatata aagccacttt attaccatcc ttttatacaa aggatggtta tcgaaagcat
     4261 caaagtagcc ctaagcaaca tgaaaaacgg ctttgacatg cgacaaaatt tccgcgattt
     4321 tgaccaatag tattataatt gcaaataaaa gaggaggtaa aaaatgggga aaaactttct
     4381 attcacttca gaatcagtaa
//
LOCUS       DAONUO010000170         4387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14207_length_4387_cov_3.593721, whole genome shotgun sequence.
ACCESSION   DAONUO010000170 DAONUO010000000
VERSION     DAONUO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14207_length_4387_cov_3.593721"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PK768_06820"
     CDS             <1..604
                     /locus_tag="PK768_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQA60718.1"
                     /translation="GYADLWDSSLKNSIGIIANYRVINGMALKVLGESYNTEDIATIE
                     AAGEKLLELAPNIRLIKDDNLQDDLLTGEVGVAVMYTSQVTMAKLSKPELRMVFPSEG
                     IGFGIMAQFIPSNAPHPDAAHKFIDYILQPEVSARCFEWMGFYCTNKAAEEYITEEYK
                     EFLTLPTNFTGDMEMIQRISPEAEEVHTKIWTKFKSETGN"
     gene            694..1377
                     /gene="sfsA"
                     /locus_tag="PK768_06825"
     CDS             694..1377
                     /gene="sfsA"
                     /locus_tag="PK768_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HQA60719.1"
                     /translation="MLIYNNVRQAKFLNRPNRFIANIELDGKQEACHVKNTCRCKELL
                     VPNATVYVQELESSKRKTKYDLISVYKGRRLINIDSQAPNKVFHNWVLETNFFKDVTL
                     IKAEVKYKNSRFDYYIETLNSKIFVEVKGVTLEENSVALFPDAPTERGVRHIYELISS
                     LEEGFEAWIVFIIQMEGIRYFTPNVKTHKEFGVALEEARRRGVNIIALDSIVTKESIK
                     AGDFVKVKL"
     gene            1473..1940
                     /locus_tag="PK768_06830"
     CDS             1473..1940
                     /locus_tag="PK768_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQA60720.1"
                     /translation="MNYPKADIFKRFIAYLIDGIISSILIYVPVLGGIVSTVYILTKD
                     TIAYEITKNPDFKNRSIGKKIMGLEVVSMEGKDVNWEISIRRNLPLAVASICSIVPVI
                     GWLIGMVVGVIIGIIEIVLVLTDNNGRRLGDRLANTQVVYSESVHKGNDIIDI"
     gene            2128..2763
                     /locus_tag="PK768_06835"
     CDS             2128..2763
                     /locus_tag="PK768_06835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQA60721.1"
                     /translation="MRIIDLSHPYKMGMTLFPGTPPMDIKQIAQIDEGGYRVTDFHSI
                     VHTGTHCDAPAHCLKGARTMDEIPLNEFVGYAVIVDVNVDDEKEIKADVLKNYDIKPG
                     DIVLLRTGYSKYWNTDKYVDDSPYLSEGMAQALVDLKIKAIGMDFISPDKVEDNTAPV
                     HKIFMRNNVHIIENLNRLDEIDSPRVFFAAAPVLIDKADGGFTRAFAVIQD"
     gene            2942..>4387
                     /locus_tag="PK768_06840"
     CDS             2942..>4387
                     /locus_tag="PK768_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HQA60722.1"
                     /translation="MKALKKTVIFILLVSLCAFFLAACSGDEETEAPQSDEAVLERNG
                     QKISFRGLSFTPWDVDEAVSLVGEQVHKPYTIMLYLNGSDLETEYGMATGDLIEIVRS
                     AFNDENIHLLILTGGALQWQNPVVPNDTCVIYQATGGDLYSIVDIGQRSIGDAGTLTS
                     FIDFCMTAFPADKYGLILWNHGGGSIAGYGVDENFNYDGLSLLELNLALEKSQASSTK
                     LEFIGFDACLMATVETAAIAQDYAHYLIASEELEPGYGWDYSFLADLGDNPHISGAEL
                     GQIIADKFVAFYAGTDEDATLSVIDLSKVDNVLEAMGNLMSACNEDFSQATFKSLAKL
                     RNNTKVFGGGSPRDNNCDMVDICDMAAKLAPYYPDEAEAVIRAVKNAVVYNKNSEGVE
                     GAAGLSTYYVFGGKEQAETAIEIYKSLGMNPHYTNFLVNFASELIGESIAPLDFSEQT
                     PEMNEDGDYTIKLTQEELDNLLEIYFTVWEPV"
BASE COUNT         1337 a          834 c         1036 g         1180 t
ORIGIN      
        1 aggttatgcc gatctttggg acagctccct gaaaaacagc atagggatca tcgccaatta
       61 tcgtgtaata aatggcatgg ctttaaaagt cttaggtgaa agttacaaca ctgaggacat
      121 agcgacaatt gaagcggcag gagaaaagct attagagctt gcaccaaaca tacggcttat
      181 aaaggatgac aatctacagg acgacctttt aacaggcgag gtcggtgtgg cagtgatgta
      241 cacttcccag gtgactatgg caaagcttag caaaccggaa ttgaggatgg tttttccctc
      301 tgagggaatc ggctttggca taatggccca gttcatacct tcaaatgccc cccatcctga
      361 tgctgcccac aagtttatcg actacatttt gcagcctgaa gtatccgcca ggtgtttcga
      421 atggatggga ttttactgta ccaacaaagc cgcggaggag tatataactg aggagtataa
      481 ggaatttctc acattgccga cgaattttac cggcgacatg gagatgattc aaagaataag
      541 cccggaagct gaagaagtcc ataccaagat ttggaccaag ttcaaatcgg aaaccggcaa
      601 ttaaacctag taaaaaacag ggctttctca ggtcttgcga gaaagccctg ctgaagacaa
      661 atacgcactt ttatcaagga gaaatggagc aaaatgctaa tatacaataa tgtcagacaa
      721 gccaagttcc taaaccggcc caatagattt atcgccaaca tagagttaga tgggaaacaa
      781 gaagcctgtc atgtgaaaaa tacatgtcgc tgtaaagagc ttctggtacc aaatgcaaca
      841 gtttatgttc aagaattaga aagcagcaaa cgcaaaacaa agtacgattt aatttccgtt
      901 tacaagggca ggaggctaat taatattgat agccaagctc cgaacaaggt atttcataac
      961 tgggtcctgg aaactaattt tttcaaagat gtaactttaa ttaaagctga agtaaagtat
     1021 aaaaactccc gcttcgatta ttatattgaa acattgaata gtaagatttt tgtcgaggtc
     1081 aagggtgtca ccctagagga aaacagcgtt gcattatttc ccgatgcgcc cacggaacgg
     1141 ggagtcaggc acatatatga gcttatcagt agcttagaag aaggttttga ggcctggatt
     1201 gtctttataa ttcaaatgga gggcatacgg tattttactc caaatgtaaa aacacataag
     1261 gaatttggcg tggcccttga ggaagccagg aggcggggag taaacataat cgcccttgat
     1321 tccattgtaa ctaaagagtc cataaaagcg ggagattttg taaaagtaaa gttataacgc
     1381 gacacaattt aattattttt ttcaaaatca ttgacctctt gcgggttcct gatataatgt
     1441 aatcagttag aaaaagaagg gaatgatata gtttgaacta tccaaaggct gatattttta
     1501 aacgctttat agcgtattta attgacggta tcatttctag tattctcatt tacgtgcctg
     1561 ttttaggggg tatagttagt actgtctaca tacttacaaa ggatacaata gcctatgaaa
     1621 tcactaaaaa cccggatttt aaaaacagaa gcataggcaa aaaaatcatg ggccttgaag
     1681 tcgtcagtat ggaaggcaaa gatgtcaatt gggagatttc cataaggagg aacttgcctc
     1741 ttgctgtcgc cagcatttgc agcatagttc cggtcatagg ttggctcata ggcatggtgg
     1801 tgggcgttat cattggcatt attgagattg tccttgtttt aacggataat aacggcagga
     1861 ggttaggaga taggctggcc aacacccaag tagtttatag tgaatcggtg cacaagggaa
     1921 atgatattat agacatttaa ttcaagatgt gaagggttgc agatttctta ccggcagccc
     1981 ttttattgta aaacaaaaat ctttgccatc agcatcaatt actgatagcc tcgtggtcgg
     2041 gctgagagtt gaaaatatct ctctgctaga aggaaatatc ctgcaaacag agaataaaag
     2101 aaataaaaat gattgggagg tattgccatg agaattattg acctgtcaca tccttacaaa
     2161 atgggcatga ccctgtttcc cggtacacca cctatggata ttaaacaaat tgcccaaatc
     2221 gacgagggcg gctatcgggt gacggatttt cactcaattg tgcatactgg cactcactgt
     2281 gatgctcccg cccattgctt aaaaggtgct aggaccatgg atgaaatacc cttgaatgaa
     2341 tttgtgggat atgcagtaat cgtggatgta aacgttgacg atgaaaaaga aataaaagct
     2401 gacgtcctca aaaactatga cataaaacct ggcgacatag tgttgctgcg taccggttat
     2461 tcaaaatact ggaacaccga taagtacgtt gatgactccc catatttatc tgagggaatg
     2521 gcacaggccc ttgtggattt aaaaatcaag gccatcggca tggattttat atctcccgac
     2581 aaggtggaag acaacacagc acctgttcat aagattttca tgagaaacaa tgtgcacatt
     2641 atagaaaatt taaatcgcct tgatgaaata gatagtcccc gcgtgttttt tgctgcagct
     2701 ccggtactca tcgacaaggc tgatggcggt tttactaggg cttttgccgt aattcaggat
     2761 taatggcttt ataaaaaact aagcatcttc gacaaataat ttcaaatatg taataagtcg
     2821 tcgatctcag gtagtgtata aagactacag gggttgacgg ctttaattaa caaacacact
     2881 tcctggcgat gtttcaagcc aatgccatgg caaaggatta agaaaaaaga gggggctgta
     2941 tatgaaagct ttaaagaaaa cagtaatttt tatcctacta gttagtctat gtgctttttt
     3001 cctggccgct tgctccgggg atgaggagac cgaggctcct cagtctgatg aagcggtatt
     3061 ggagcgcaac gggcaaaaga tatcttttag agggctttcc tttactcctt gggacgtgga
     3121 tgaggctgtt tccctggtag gtgaacaagt acacaaacca tacaccatta tgctgtacct
     3181 gaatggatcg gaccttgaga ccgaatatgg aatggcaacc ggagatttga tcgagattgt
     3241 ccgatcagct tttaatgatg agaacatcca tcttcttatt ctgaccggcg gcgctctgca
     3301 gtggcaaaat cctgttgtac ccaatgacac atgcgtaata taccaggcaa ccggcggtga
     3361 tttgtattct attgtcgata tagggcagcg cagcataggt gatgccggaa cattgacgag
     3421 tttcatcgat ttttgtatga cagcttttcc cgcggacaaa tacgggctta ttctctggaa
     3481 ccacggaggt ggctcgatag ccggctatgg agttgatgag aactttaact atgatggttt
     3541 atcgctattg gaattgaatc tagcactgga aaaatctcaa gcttcaagta cgaaactaga
     3601 atttattggt tttgacgctt gcttaatggc aacggtggag acagcagcta ttgcccaaga
     3661 ttacgcccat tacctcattg cttccgagga gctggaaccg ggctatggct gggattacag
     3721 ctttttggca gaccttggcg acaacccgca tataagcgga gccgaattag ggcagattat
     3781 agcggataag tttgtggctt tttacgcagg taccgatgaa gatgctaccc tttccgttat
     3841 tgatttatcc aaggttgata atgttcttga ggctatgggc aacctaatgt cagcatgtaa
     3901 tgaggacttt tcgcaggcaa cttttaagag cttagctaag ttgcgaaaca atactaaagt
     3961 cttcggtggg ggcagcccca gggacaataa ttgcgatatg gtagatatct gcgacatggc
     4021 cgcaaagttg gcgccctatt atccggacga agcggaggca gtaatccggg cggtgaagaa
     4081 tgctgttgtt tataacaaaa attcggaagg ggtagaaggc gctgcaggcc tttctactta
     4141 ttacgtgttt ggcgggaaag agcaagctga gaccgctata gaaatataca aatctttagg
     4201 tatgaatccc cactacacca attttttggt caactttgcc tctgaactta taggagaaag
     4261 tatcgctccc ctggattttt ccgagcaaac accggagatg aacgaggatg gggattacac
     4321 cataaagctg acacaggaag agttggataa tttgttggag atttacttta ctgtatggga
     4381 gccggtt
//
LOCUS       DAONUO010000171         4380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14236_length_4380_cov_3.959075, whole genome shotgun sequence.
ACCESSION   DAONUO010000171 DAONUO010000000
VERSION     DAONUO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14236_length_4380_cov_3.959075"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1187)
                     /locus_tag="PK768_06845"
     CDS             complement(33..1187)
                     /locus_tag="PK768_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA60723.1"
                     /translation="MKPDELLIQDNRKKGWFWSYNYILDRTDLDIYAKMIYMVLARHA
                     DGHNTKATVSYKELSSGAGCSIRKAKEAVSVLEAKGLVVKQNNISMSGTYGANTYVFK
                     TEERKKGNWFWSENSLVDREDLNLYEKMVYLVLVRRADGRNTKACVSYSQIAITLGCS
                     RRQAINTISSLIKKGLISEPKPGPSGKNIYFINNVSCSSRDAAVQKSGAHDAPYQNQS
                     FEAVVEKSGESGAQSNICLVNDTHGGSASGSPGVVNDTHGGSAPDAHIQERSISDQED
                     QQQDDAVFNLLVNYGISQNVARRLSAEVDLGKVKKAIEITEQAKRSNGIWKSSQGYLV
                     SILESGLLNTGPVVTNRKKNYVDRVPNDQASDQASRQYIEFIRSMWSAWS"
     gene            complement(1734..3053)
                     /locus_tag="PK768_06850"
     CDS             complement(1734..3053)
                     /locus_tag="PK768_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQA60724.1"
                     /translation="MARGSMRQRGKNSWELTIELPRIGGKRRRKIITVRAKDKREAER
                     ELNRLQAIYQEKTFTEPSNEKLGNYLLSWLETVETFEDVARSTLKRYREIIVKHLIPA
                     LGEIPLSKLRPKHLTDYYTEALKNGRIRDNGPLSKTTVHHHHSVLRKALNDAKAQKLI
                     HENPAADAKAPSMASRDVEVLMPDQLNKLMDRLKSSYVYLPSYISMVTGARLSEVLGL
                     KWEDIDFKNKTISIERSLLWDDEGWYFNKNLKSNYARRQIDIEDEDIEVLKHYRELQL
                     QQKLEKGPEYNDLGLVCCKDDGTPLRGDNVTKRFKIIARSIGLGNFNFHGLRHTHATL
                     LLMSGVPINIVSERLGHYSAEFTLKVYIHKVKSMQKVASKTFRAILDACRDSQEECGL
                     SLKMADTEEHVRDGSEKDSSKAEARNEKRVGRQLVGKSKVSYLKLVK"
     gene            complement(3141..3323)
                     /locus_tag="PK768_06855"
     CDS             complement(3141..3323)
                     /locus_tag="PK768_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA60725.1"
                     /translation="MSDSELITISEASEILNVSRATAYRLAAHREIPVVMLGGCVRIH
                     RRKLIELLENKVEMKV"
     gene            complement(3522..>4380)
                     /locus_tag="PK768_06860"
     CDS             complement(3522..>4380)
                     /locus_tag="PK768_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQA60726.1"
                     /translation="MTDYESFLKQKDVSTNTIASYLADLEAFKNWYRGTTGEEFDPGA
                     IGPIDVAEYKRYMLNRNRKPATINRHLASLSAYFRWAVERGLSPQNPVDGIKSVKQER
                     LAPKWLERREQLSLLRAVQASKKPRDIALINLLLHTGLRVSEVCALDLDDVFLRERSG
                     WVVVRSGKGGKRREVPLNATVRSSLADWIEARCREPGPLFFGQKGNRMTSRGIEHLIA
                     KYARIAGVEKVTPHSLRHTFCKSLIDSGESLDRVAVLAGHESLNTTARYTRPSVGDLQ
                     KSVDKLAWE"
BASE COUNT         1107 a         1172 c          822 g         1279 t
ORIGIN      
        1 ttccatgact atcctgattc actttcttgt catcaagacc acgcactcca catgctacga
       61 ataaactcaa tatactgcct acttgcttga tcggacgcct gatcgtttgg aacacgatct
      121 acataattct ttttacggtt tgtaacaact ggaccggtgt ttagcagacc actctccaat
      181 atgcttacga gatagccctg actggacttc cagatgccat tggaccgctt agcctgctcg
      241 gttatttcta ttgccttttt taccttcccc aaatccactt cggcagataa ccttctcgcc
      301 acgttttgcg aaataccata attgactaga agattaaaaa ccgcatcatc ctgttgttga
      361 tcttcttgat cgcttattga tctttcttgt atgtgtgcat ccggtgcact acccccgtgc
      421 gtgtcattca ccacccctgg tgaacctgat gcactacccc cgtgcgtgtc attcaccagg
      481 caaatgttcg attgtgcacc tgattcacca cttttttcta caaccgcttc aaaagactga
      541 ttttgatatg gtgcatcatg tgcaccactt ttttgaaccg ccgcatcacg actggagcag
      601 ctgacattgt taataaaata aatgttcttg cccgatggcc cgggcttggg ctcagagatt
      661 aaaccttttt taatcaggga agatatcgta ttgatcgcct ggcgacggga acagcctaaa
      721 gttatggcta tctgggaata agatacgcac gcctttgtgt ttctaccgtc ggcccggcgc
      781 accagcacca ggtagaccat cttctcgtaa aggtttaagt cctcacggtc aaccagggag
      841 ttctccgacc aaaaccagtt gcctttcttc cgctcctccg ttttaaagac ataagtgttg
      901 gcaccgtagg ttccagacat ggaaatatta ttttgcttca cgaccaaacc ttttgcttcc
      961 aagaccgata ccgcttcctt agccttccta atcgaacacc ctgcaccaga cgacaactct
     1021 ttatatgaaa ctgtagcttt ggtgttatgg ccatctgcgt gccgcgccag aaccatatag
     1081 atcatcttgg catatatgtc cagatcagtc cggtcaagta tgtaattata agaccaaaac
     1141 cagccttttt ttcgattatc ttgaatcaat agctcgtccg gtttcatcct gcttcctccc
     1201 ttcttttttg acataaaaaa aagaagccct taacgggccg ccttgatttg tctataaaaa
     1261 tctataatta aagcacagct tttaaccgtg catcatagtg ttcaggttca tcgaatttga
     1321 tagggtagcg gtctttatct tttggatctc caaaaacgaa ttgccgacca attgccgacc
     1381 ataacaccag ataatacatg aaacgacatt taacgacaca caacagatga taaaatatta
     1441 aatctaataa aaccttcccg gccataataa atataagcat ttatgagact gcgctatata
     1501 gcatgatact ttttaataat attgagaagg cataaatagc ctatagccgg aaaactccaa
     1561 aaccgtaggt tgcgggttca agtcctgtct cccctgccag gtttatagcg ttttgttgcg
     1621 gtgcaagctt atgcttgcgt tggccctgaa accctttatt tgtgggcttt tcggggttct
     1681 ttttttatcc gtagcaagga aaaacacgac tactcccctt ccatttaata ccatcatttt
     1741 accagtttca ggtagctaac tttggacttg ccgaccaatt gccgaccaac ccgtttttcg
     1801 tttcttgctt ctgctttact actatctttt tcactaccgt ctcttacatg ctcttctgta
     1861 tcagccatct taagagacaa cccgcactcc tcttggctat cacggcaagc atccaatatg
     1921 gctctaaagg tcttagatgc aactttctgc atggatttta ccttgtgaat atagactttc
     1981 aaggtgaatt ccgcagagta atgacctaaa cgctcactga caatattgat tggtacacca
     2041 ctcattaaaa gcagggtcgc atgggtgtga cgcaagccgt gaaaattgaa gtttcccaaa
     2101 cctatacttc tggctattat tttgaatcgt ttcgtaacgt tgtctcctct aagcggggta
     2161 ccatcatctt tgcagcacac caagcctaaa tcgttatatt ccggaccctt ctccaacttt
     2221 tgctgtaact gcagctccct ataatgcttc aaaacttcaa tatcttcatc ttcaatgtct
     2281 atctgcctgc gagcgtaatt gctcttcagg ttcttgttaa aataccagcc ttcatcatcc
     2341 cacaacaagg aacgctcgat gcttattgtc ttgttcttaa aatcaatgtc ttcccattta
     2401 agccctagca cttcactaag tcttgctcct gtaaccatag aaatgtagct cggcagataa
     2461 acgtagctgc ttttcagtct gtccatcagc ttattcagtt gatccggcat taacacttct
     2521 acatctcttg atgccatact cggagcctta gcatctgccg ccgggttctc atgaatcagt
     2581 ttctgggctt ttgcatcatt caaggctttc cttaaaacgc tgtggtgatg atgaacggtc
     2641 gttttagaca aaggaccatt atcacgtatc cgaccatttt tcaaggcttc ggtgtagtaa
     2701 tccgtcaaat gtttcggcct aagcttcgac aacggtattt ctcctaatgc agggatgaga
     2761 tgttttacga ttatctccct gtaacgcttg agagttgaac gggcaacatc ttcaaaagtc
     2821 tcaactgttt ccagccaaga caagagatag ttacccaatt tctcattgct cggctcagta
     2881 aacgttttct cttgatatat tgcctgcaag cgatttaact ctctttcggc ctcccgctta
     2941 tccttagcac gtacggtaat tatcttcctt cgacgttttc ctcctattcg tgggagttct
     3001 attgtaagct cccatgaatt tttacccctc tggcgcatac tgccccgggc catatttaat
     3061 tttgcacctc cttatatatg gccgggaagg tgtaaagtac ttcgataaaa caaagcacta
     3121 tccctcccag tcaactaaat ttaaaccttc atctctacct tgttttcaag aagctcaatc
     3181 agcttacgcc tgtgaatacg aacacatcct ccaagcatta ccaccggaat ctctctatga
     3241 gctgctaatc tgtaagctgt tgctctgctc acattcaaga tttcggatgc ctccgaaata
     3301 gtaattaatt cagaatcgga catttttcac agcctccttg caaaaaatag taaaatcctg
     3361 cgcttaccca ggaaataaaa atacaggcaa gcaaacaagc tcacctgtac ctttaccggg
     3421 cataccccta atcgttaaag ccaaacttta aaacagttcc acttcactcc tttcttttgt
     3481 tgcgtgccat aagttgcaat aagaataatt ttacatgtat ctcactccca ggccagctta
     3541 tcaacgctct tctgcaggtc gccaacgctg ggcctggtat aacgggcggt agtgttcaaa
     3601 ctttcatggc ccgccaggac cgccacccgg tcaaggctct ccccagaatc aatcagagac
     3661 ttgcagaaag tatgcctcaa ggagtgcggg gtcactttct ccacgccggc aatacgggca
     3721 tacttggcaa tcaggtgctc aatgcccctc gatgtcatcc ggttgccctt ctgaccaaaa
     3781 aacagcggcc cgggctcccg gcacctcgct tctatccagt cggccaaaga cgacctcacg
     3841 gtagcgttca ggggcacttc ccttctcttg ccgcctttcc ctgatctaac aaccacccat
     3901 ccgctgcgct cacgcaggaa aacatcatcc aggtccaggg cgcagacttc ggataccctc
     3961 aaacctgtat gaagtaaaag attgatcagg gcaatatccc ggggtttctt gctcgcctgt
     4021 accgcccgca gaagggaaag ctgctcccgg cgctccagcc atttcggggc cagcctctcc
     4081 tgcttcacgc tctttatgcc gtcaaccgga ttctgaggcg acaaacccct ctcaactgcc
     4141 cacctgaaat acgccgacaa ggaagccagg tgccggttaa tagttgctgg cttcctgttc
     4201 cggttcagca tgtatctttt gtattccgct acatcaatag gaccgatagc gcccgggtca
     4261 aactcttcgc cagtagtgcc ccgataccag ttcttgaaag cctcaagatc agccaggtaa
     4321 gaggctatcg tatttgtact cacgtctttc tgcttcaaga aagattcata atcagtcatg
//
LOCUS       DAONUO010000172         4375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14258_length_4375_cov_3.692593, whole genome shotgun sequence.
ACCESSION   DAONUO010000172 DAONUO010000000
VERSION     DAONUO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14258_length_4375_cov_3.692593"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="iscB"
                     /locus_tag="PK768_06865"
     CDS             <1..1038
                     /gene="iscB"
                     /locus_tag="PK768_06865"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HQA60727.1"
                     /translation="AAEIEHKGKQVRAKMLQRRQLRRGRRYRKTRYRKPRFLNRRKPE
                     GWLPPSLESRIANIVTWVNRLIKLCPISAISIELVKFDTQKLQNPEISGVEYQQGELF
                     GYEVREYLLEKWGRKCAYCGRENVPLEIEHIVPRSRGGTDRISNLTLACRKCNQKKGN
                     LTAEEFGFSEIQKRARRPLKDAAAVNTTRWAVYRKLSETVVPVESGTGGMTKYNRTKL
                     GLKKEHWLDAACVGESTPDDLTICAKSVLKIKAVGHGSRQRCITDRYGFPKAYLTKKK
                     TYMGFATGDIVKAVVPRGKYAGIYIGRIAIRQRPSFRLNGVDVHPKYLTILQRGDGYE
                     YEYMAAHSPSQ"
     gene            1163..3946
                     /locus_tag="PK768_06870"
     CDS             1163..3946
                     /locus_tag="PK768_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQA60728.1"
                     /translation="MIYRFYESGMPEVFSDFIVRCNNEIRAISLIGTEIAVKSYRAKF
                     LDNNMARRSLPFYMMAEEETYPRTIYKPADRFINMPAQKTGQGYQIFMYSENLLFGLC
                     HEDLLSMLYKHLYDLNIPCPTPGEEIFAECLELILENALEISYLVPCKCLGSLPESAV
                     FYYINQVWLSDFRDTAAEIISRFITEEISSNEILDSVEGIADYIEKYSAEIGEKVGKC
                     VTYLHTPGDYDDEIFNKMSRKLFPQQKDIAVAVAKRLKTGKNALVSGQMGVGKTSIGI
                     AACQYHADGEPYRAIVTCPGHLVEKWAREVLTILPDAVVHTIGTHEKLKPWQEFYATY
                     RYSTTPAGPEYWIVSNEALRGGYITRPGYTIKKKKAFDVEAQEHVFIEVCTCPKCGHE
                     LDYPQKNENRDEVIVTLHPGDFETHNSLNHKCRHCSEPLWQADNNRKGYRKVSIADLI
                     KKRIPKNYFKYYIADESHKYKGSTAQGLAFGALISKCKHTIGMTGTLADGYANGLYYL
                     LWRLNPTKFKEQKFYHDEESRSRFQMEYGFWEKIVKTRGSVYGKTSKARNTRTQVKLL
                     PGYTINTFPEWLLETTVFLKLSDVAPHLPPKHEFVNVVEMDEELRESYKEIENSLLAS
                     IKEGGSQMASIMLHTCLSYPDLANGPDAINVVKPDFQFYLPLPELDTTKLYNKERELQ
                     KIIKAELALGRKCLVFTTYTNKRDCLPRLEWVASQIPGAKVQVIRSTTVNTRKREAYV
                     KEKLNQEGKNVLICHPELVETGLDLLECPTVIWVQTGYIPSTVRQASARSWRIGQDAE
                     VKVIFLCYGDTLQEKCFQLVGSKLNAAGILEGHLSNEGLRSFGDNGDMVDILSLLKDN
                     IVTVNSNDIFQEYKAEVAALLSKDTLSAEETHRLKTLSDILATQEIDLSHLSPRKRKK
                     LMQQADSQLLLFA"
BASE COUNT         1344 a          925 c         1117 g          989 t
ORIGIN      
        1 gctgcagaga ttgagcacaa aggaaagcaa gtcagagcaa aaatgctaca gcgcaggcaa
       61 ctccgcaggg gtaggagata ccgcaagacc cgctaccgca aaccgagatt cttgaaccgc
      121 agaaaaccgg aagggtggct ccctccaagc cttgaaagca gaattgccaa tattgtcacc
      181 tgggtaaacc ggctgataaa gctgtgcccc atttcggcga tatctattga acttgtcaag
      241 ttcgacactc agaaactgca gaatccggag ataagcgggg ttgaatacca gcaaggcgag
      301 cttttcggtt acgaagtgcg ggagtacctg ctggagaagt gggggcggaa gtgtgcctac
      361 tgtgggaggg aaaatgtacc actggaaatt gaacacatcg tccccaggtc tagaggtggg
      421 acggatagga tatcgaattt gactctagct tgtcggaagt gcaaccaaaa gaaagggaac
      481 ctaacagcag aggagttcgg attttctgag attcaaaagc gggccaggag accgctgaaa
      541 gatgctgctg cagtcaacac gacacggtgg gcggtataca ggaagctgag cgagacagtg
      601 gttccggtag agtccggcac tggtggaatg acgaagtaca accgaacaaa gctcggttta
      661 aagaaagaac actggctgga tgcagcctgt gtcggggaaa gcacgcctga tgacctgacg
      721 atatgcgcta agtccgtact gaaaatcaaa gcggtcggac acggaagcag gcagagatgt
      781 attactgata ggtacgggtt ccctaaggca tacttgacca agaagaaaac ctacatgggg
      841 tttgctactg gcgacattgt aaaagctgtt gtgccgagag ggaagtacgc cggaatttat
      901 atcggcagga tagcgataag gcagagaccg tcgttccggc tgaacggcgt cgatgttcac
      961 ccgaagtatt tgacgattct tcagagaggc gatgggtacg aatacgaata catggcggcg
     1021 cattccccct cccaatgaat tgggaggcac cctgcgccga agccttgtgg aaaacatata
     1081 agttaaaact ccgagaactt acccggaatc gggcagaaaa accaacctgc ccgacaacgg
     1141 gcaggtttaa ggaggtagaa aattgatata tcgtttctat gagtcgggta tgcccgaagt
     1201 cttttcggat ttcattgtga gatgcaacaa tgagattagg gcaatttccc tgataggaac
     1261 agaaatagcc gtaaaaagct atcgagccaa gtttttagac aataacatgg ctcggagatc
     1321 cctgccgttt tatatgatgg cagaggaaga aacatatccc agaacaatat acaagccagc
     1381 tgacagattt ataaatatgc cagcccaaaa gacagggcag gggtatcaaa tcttcatgta
     1441 tagcgaaaat ttgctttttg ggctatgcca tgaagatttg ctgtcaatgc tgtacaaaca
     1501 cctgtatgac ctcaatatcc cgtgccctac gccgggagag gaaatttttg cagagtgttt
     1561 ggagttaatt ctggaaaacg ccctggaaat ctcttatctt gttccatgca aatgtctggg
     1621 cagcctacct gaaagtgcgg tattttatta tatcaatcag gtatggttat ccgactttcg
     1681 ggacaccgcg gcagaaataa tcagcaggtt tattactgaa gaaatcagca gtaatgaaat
     1741 actggatagt gtagaaggca tagccgacta catcgaaaaa tactctgccg agatagggga
     1801 aaaggtaggt aaatgcgtaa cctatctgca cactcctggc gattacgatg atgaaatatt
     1861 caataaaatg agccgcaagc tgtttcctca gcaaaaggac attgctgtag cagtcgcaaa
     1921 gcggttaaaa accggcaaaa acgcgctggt aagcggccag atgggtgtcg ggaaaacgtc
     1981 aatcggaata gcggcgtgtc aataccatgc tgacggggag ccgtacaggg ctatagtcac
     2041 ttgcccggga cacctggtag aaaagtgggc aagagaagtt ttgacaatac tccccgatgc
     2101 ggtggttcat actataggca cccacgaaaa gttgaagccc tggcaggagt tttatgccac
     2161 ctacaggtat agcactactc cggccgggcc ggaatactgg attgtttcaa acgaagcctt
     2221 gcgtggcggt tatataacca ggcctggcta cacgataaag aagaaaaagg cttttgatgt
     2281 ggaggcgcag gaacacgttt ttattgaagt ctgcacctgt cctaaatgcg ggcacgaact
     2341 agattatcct caaaagaacg aaaacaggga cgaggtaatc gtcaccctgc atccggggga
     2401 ttttgagact cacaacagtc ttaaccacaa gtgccggcat tgtagcgaac cactgtggca
     2461 ggccgacaac aacaggaagg gataccgcaa ggtatctatt gcagacctga ttaaaaagcg
     2521 tattcctaaa aactatttta aatattacat cgctgatgaa agccacaagt acaagggttc
     2581 aaccgcacaa gggctggcct ttggcgcttt aatcagcaag tgtaaacaca ccatcggtat
     2641 gaccgggact ctcgctgatg ggtatgccaa tgggctttat tatctcttgt ggaggttaaa
     2701 ccccacaaaa ttcaaagagc agaagtttta ccatgacgaa gaatcccgtt ccaggttcca
     2761 gatggaatac ggtttttggg agaaaatcgt caaaaccagg ggtagtgttt atggcaagac
     2821 cagtaaggca aggaacaccc gaacacaggt taagctgttg ccagggtaca ccattaacac
     2881 attccctgaa tggctgttag aaacaacagt ctttttgaaa ttaagcgatg tagcccctca
     2941 tctgccgccc aaacatgaat ttgtaaatgt agtagaaatg gacgaagaat tgagggaaag
     3001 ttacaaagag atcgagaaca gcttattggc aagtatcaaa gaaggcggca gccaaatggc
     3061 ttccataatg ctgcatactt gcctgagcta tcctgatctg gctaacggcc ccgatgcgat
     3121 taatgtggta aaaccggatt ttcaatttta tctgccttta cctgaactag atacgacaaa
     3181 gctatacaac aaagaaagag agctgcagaa gatcataaag gcagagttag ccctaggcag
     3241 gaagtgtctc gtattcacta cctataccaa caaacgtgac tgtttgccga ggttggagtg
     3301 ggtagcgagc caaataccag gtgctaaagt gcaagtaatc cggtcaacca ctgttaacac
     3361 ccgcaagagg gaggcgtatg tcaaagaaaa actcaaccaa gaaggcaaga atgtgctgat
     3421 atgccatcct gagctggttg aaacagggtt agatctgtta gagtgtccta cagtaatatg
     3481 ggtacagaca ggctatatcc cctcaacggt aaggcaagcc tcagctcgca gttggcgcat
     3541 agggcaggat gccgaagtaa aggttatatt cctttgttac ggggatacct tgcaagaaaa
     3601 atgcttccag ttagttggga gcaagctgaa cgcagcaggt attctcgaag gccatttatc
     3661 aaacgaaggt ttgcgttcgt tcggtgacaa cggtgatatg gtcgatatat tgtccttatt
     3721 gaaagacaat atagttaccg taaactccaa cgatattttc caggaataca aagcggaggt
     3781 tgcggcacta ctcagtaagg acactttgag tgcggaagaa acccatagac taaaaacatt
     3841 atctgatatt ctggcgactc aagaaataga cctttcccac ttgtcaccaa gaaagcgcaa
     3901 gaagttaatg cagcaggcag atagtcaatt actactattt gcttaaatac gggttgcaca
     3961 aaaagcatta tagagatata ataagaggca acaaatttgt cacaaaattt gtgttgctac
     4021 ccgtgtgccc tcatataagc cgctctaaag agcggctttt ttattattac acctattacc
     4081 cccaacctct cattcccggc cgtggtcatc agaccgcggc cttttttatg aggtcttttt
     4141 aaaaaatact gcgaataacg cggtgaaagg gtacgcgcgt accgaaagga ggtaaagaat
     4201 gagtcattgg tttacgttaa aaaccaaact taatagcgaa tcagctatta aagctggcgc
     4261 aaggcggtta gggtacatgg tagtccataa ccgtatgtgc aggggttaca atggcaacga
     4321 aaccctatgc gacctggtta tgaagttgcc cgggcagtat gatataggat tccag
//
LOCUS       DAONUO010000173         4344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14362_length_4344_cov_3.972954, whole genome shotgun sequence.
ACCESSION   DAONUO010000173 DAONUO010000000
VERSION     DAONUO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14362_length_4344_cov_3.972954"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK768_06875"
     CDS             complement(<1..516)
                     /locus_tag="PK768_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQA60729.1"
                     /translation="MDDQLIDSFLNYLRATKAKSENTTKAYAEDLFQFLEYLKLRKLS
                     EPVLLNINHLHIRGFLAHLRDREISKRTVARKLSTLRSFYKYLIVEGFTKENVAKMVQ
                     APKTSKRLPLFLYPQEIERLLSAPKKDILGIRDRAILELLYATGMRVGELVALKIGDI
                     NFGANYIIVFGK"
     gene            complement(633..1931)
                     /gene="trmFO"
                     /locus_tag="PK768_06880"
     CDS             complement(633..1931)
                     /gene="trmFO"
                     /locus_tag="PK768_06880"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047151 -
                     methylenetetrahydrofolate-tRNA-(uracil-5-)-
                     methyltransferase (FADH2-oxidizing) activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HQA60730.1"
                     /translation="MVAVKVIGGGLAGCEAAWHLAENGIKVDLYEMRPSKSTPAHHTD
                     KLAELVCSNSFRSNEITNAAGLLKEEMRILGSIVMKVAEETSVPAGAALAVDREKFSS
                     KITKMIINHPNIRFHVEEIREIPEPPTIVATGPLTSEAMSKAVAELLGSEHLYFYDAA
                     APIITSESIDWEKAFCGSRYDKGDADYVNLPLTQEEYDIFYNELIAAKTHPLRDFEKP
                     MFFEGCMPIEEMAKRGYKTLLFGPLKPVGIIDPRTRKQPFAVVQLRKENNEGTLFNIV
                     GFQTSLKWGEQKRVFGLIPGLEDAEFIRYGFIHRNTFILSPLHLKATLEYKGISGLFF
                     AGQITGVEGYIESAASGIIAGFNMKRLLKGQRPLEPPAESVIGALLNYVSSADPANFQ
                     PMKANFGILPQLPYKVKDKTLRKKLLAERALEFMSKYVKL"
     gene            complement(1950..4019)
                     /gene="topA"
                     /locus_tag="PK768_06885"
     CDS             complement(1950..4019)
                     /gene="topA"
                     /locus_tag="PK768_06885"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQA60731.1"
                     /translation="MSKSLIIVESPAKAKTLSKYLGKKYKVAASMGHVKDLPKSQLGI
                     DIESNFEPKYITIRGKGPLLNNLRKEAAKADNVLLATDPDREGEAISWHLASTLGLPE
                     DKPCRIEFHEITKDAVLESLKHPRTINKDLVEAQQARRILDRLVGYKISPILWRKVKW
                     GLSAGRVQSVAVRIICDREKEIRDFVPNEYWTIDADLTDGSNKIRARLHSKNDDKLVI
                     ENKEQAEKILKEIENERFQVIEVKKGTRKRSPSPTFTTSSMQQEASRKLGFTAKKTMM
                     VAQQLYEGLDVKGEGTVGLITYMRTDSTRVSDQAKREAAAYIESNFGKDYLGHGDASR
                     KSTKNVQDAHEGIRPTSVFRSPDKVKDSLTKDQFLLYKLIWERFTSSQMAPAIYDTIS
                     IRIKAGEYIFVVSGSTLKFPGFMLLYTEGSDEESEREENHIPDLRKGQTLELLKLVPE
                     QHFTQPPPRYTEAMLVKVLEEKGIGRPSTYAPTIDIIQKRGYVEKERGRFKPTELGEI
                     VVQILQEFFREIVDIDFTAEMEEQLDKIESGHQNRQEVLKSFYSTFDKQVKTAEQELE
                     KVKIEDEVSEEKCELCGRNMVIKRGRYGKFLACPGFPECKNTKPIVTEIGVSCPDCGN
                     PLVVRRTKRGRIFYGCSDYPDCKFVSWNKPSGKSCPQCGSFMVIKNSKGKEYEVCSNK
                     ECGYKAK"
     gene            complement(4088..>4344)
                     /locus_tag="PK768_06890"
     CDS             complement(4088..>4344)
                     /locus_tag="PK768_06890"
                     /inference="COORDINATES: protein motif:HMM:NF036806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HQA60732.1"
                     /translation="SVEDILDELYLERQANMINEGAIDFQKLTRSEKILYELIDYHPI
                     QIEELIEMSQLDSNKTNAVLTRLELKGLILALPGGYYVRN"
BASE COUNT         1123 a          951 c          757 g         1513 t
ORIGIN      
        1 tttgccaaaa acgataatat aattggcacc aaaatttata tcaccgattt tcagtgcaac
       61 tagttctccc actctcattc ccgtggcata taaaagctca agtattgctc tgtctctaat
      121 tcccaatatg tcttttttgg gcgctgaaag aagtctctct atctcttgcg ggtataagaa
      181 taaagggagt ctttttgatg tctttggtgc ctgaaccatt ttggcaacgt tttctttggt
      241 aaaaccttcc acaatcaaat acttataaaa acttcgcagc gtagacaatt ttcttgctac
      301 agttctcttt gatatctccc tgtccctaag atgtgccaaa aagcctcgga tatgtaagtg
      361 atttatattt aagaggacag gttctgatag ttttctcagt tttaaatatt caagaaactg
      421 gaagaggtct tctgcatatg cttttgtcgt attttcggac ttggctttgg tggctcgtag
      481 gtagtttaag aagctgtcga ttagttggtc atccatatca tcaaacctct caattaacat
      541 ttattatagt atcataagtt taaaaattat gcaagattaa atttcgaaat tatttatatt
      601 ttggcttgtt attttttttt gaaaaaactc tttcataatt tgacatattt cgacataaac
      661 tctaaggccc gctcagccaa aagttttttt cgaagggttt tatcttttac cttataaggg
      721 agttgtggta ggataccgaa atttgctttc attggttgaa agttagcggg atctgcagaa
      781 gatacataat tcagcaaagc tccaattacg ctttctgcgg gcggttcaag tggtctttgg
      841 cctttaagaa gtcttttcat gttaaatccc gcaattatac cggaagctgc tgattcaata
      901 taaccctcaa cacctgttat ctgccctgca aaaaataagc cacttattcc tttatactct
      961 aatgtggctt ttaaatgaag aggacttaaa ataaaagtat ttctatgaat gaagccgtac
     1021 cgtataaact cggcatcttc caaaccggga attagtccga agactctttt ttgttctccc
     1081 cactttaaac tggtttgaaa gccgacaata ttaaataaag taccttcatt attctctttt
     1141 ctcaattgga ctacagcaaa gggctgcttt cttgttctcg gatctatgat cccaacgggt
     1201 tttaaaggac cgaaaagtaa cgtcttgtag cctcgttttg ccatttcttc aataggcatg
     1261 cagccttcaa aaaacatggg tttttcaaag tcccttaagg gatgcgtctt ggctgcaata
     1321 agttcattat aaaaaatatc atattcttcc tgtgtgaggg gtaaattgac ataatcggca
     1381 tcccctttat cgtatctgga gccacaaaaa gccttttccc aatcgattga ttcacttgtg
     1441 ataattggcg ctgctgcatc gtagaagtaa aggtgctcgc ttcctaatag ctcggcaaca
     1501 gctttagaca tagcttcaga ggtaaggggc cccgttgcta caattgtagg aggctccgga
     1561 atttctctga tctcctctac atgaaacctt atatttggat gatttattat cattttagtt
     1621 attttggatg aaaacttttc cctatctacg gctaaggctg caccagctgg aaccgaagtc
     1681 tcttccgcga ctttcataac aattgagccc aatattctca tttcctcttt taggagacca
     1741 gcggcgtttg ttatttcatt ggacctgaaa gaattgctac acactaattc tgccagtttg
     1801 tcggtatggt gtgcaggagt agacttactg ggtctcattt catataaatc aaccttaatt
     1861 ccgttttctg ccagatgcca tgcggcttcg catcctgcta gaccgccccc tatcacttta
     1921 acagctacca tagcatcacc tctagaaagc tactttgctt tatagccaca ctctttgttt
     1981 gaacaaactt cgtattcttt tcctttgcta tttttaataa ccataaagct tccgcattgt
     2041 ggacatgatt tcccgctcgg tttattccaa gaaacaaact tgcaatcggg gtaatcggaa
     2101 caaccgtaaa atatccttcc acgcttagtc ctccttacaa ccaaggggtt tccgcaatct
     2161 ggacagctta cgcctatctc ggtcacaatg ggcttagtgt ttttacattc agggaagcca
     2221 ggacaggcta ggaacttgcc gtatctgcct ctcttgataa ccatgttcct gccgcagagt
     2281 tcacacttct cttcacttac ctcgtcttcg atctttactt tttcgagttc ctgttcggca
     2341 gtttttacct gcttgtcaaa agtgctataa aaagacttta agacttcctg tcgattttga
     2401 tgacctgact ctattttatc aagctgttcc tccatctcag cggtaaagtc tatatctaca
     2461 atttctctga aaaattcttg caaaatttgc accactattt ctcccagttc tgtaggttta
     2521 aagcgccctc tctccttctc cacatatcct cgtttttgta ttatatcaat cgttggagca
     2581 taggtgcttg gcctgcctat acctttttct tcgaggactt tgaccagcat tgcttcagta
     2641 tacctggggg gtggttgcgt aaaatgctgt tcaggaacta actttagcaa ttctagggtc
     2701 tgtccttttc ttaaatccgg aatgtgattt tcttccctct cagattcttc atcggatcct
     2761 tccgtataca gcaacataaa gccggggaat ttaagagttg aacctgaaac tacaaagata
     2821 tactctccgg ctttgattct gatagatatt gtatcataaa tggcaggagc catttggctg
     2881 ctggtaaatc tttcccaaat aagtttgtac agcaaaaatt gatccttcgt aagagaatcc
     2941 tttactttat caggtgacct aaatactgaa gttggtctta tgccttcatg ggcatcctgt
     3001 acattttttg tgcttttgcg tgaggcatct ccatggccta aataatcctt accgaagttg
     3061 ctctcaatat acgcggcagc ttcacgtttt gcttgatcgg aaacccgtgt agagtcggtt
     3121 ctcatatagg taataagacc tacggtgccc tcgcctttca catccaaacc ctcatataac
     3181 tgttgggcaa ccatcatggt tttctttgca gtaaagccca actttctaga ggcttcctgc
     3241 tgcatgctgc tggttgtgaa agtaggagaa gggcttcttt ttcttgttcc tttttttact
     3301 tctattactt gaaatctctc attttctatt tcttttaaga ttttctctgc ctgttctttg
     3361 ttttcaatta caagcttatc atcatttttt gaatgcaaac gtgcacgaat cttattgcta
     3421 ccgtctgtta aatctgcatc aattgtccag tattcatttg gaacaaaatc cctaatctct
     3481 ttttctctat cacatataat tctcacagcc accgactgta ctctgcctgc actaaggccc
     3541 cacttgactt ttctccataa aattgggctt atcttatagc ctactaatct gtccagtatc
     3601 ctcctggcct gctgtgcctc aaccaagtcc ttatttattg tcctcgggtg ttttaacgat
     3661 tctagcactg catcttttgt aatttcatga aactcgatcc tacagggttt gtcctcaggc
     3721 aagcctaatg tgcttgccaa atgccatgat atggcctctc cctcgcggtc ggggtcggta
     3781 gctaataaga cattatctgc ttttgctgct tcttttctta aattgtttaa taagggccct
     3841 tttcctcgta tggtaatgta cttaggttca aaattcgact ctatatcgat acctaattga
     3901 cttttaggca agtcttttac atggcccatc gaagctgcta ccttgtattt ctttcctaag
     3961 tatttgctta aggtttttgc tttcgcaggc gattcaacaa ttattaaaga ttttgacatt
     4021 aaaaccacct tttttacttt gtaactaaca accagtaata tattataata actttatctc
     4081 gcaaaagtca atttctaaca taataacctc cgggaagagc aagtatcaaa cctttaagtt
     4141 caagccgggt taaaaccgca tttgttttat tggaatcaag ttgcgacatt tctataagtt
     4201 cttcaatttg tatcgggtgg taatctatca gttcataaag gattttttct gatcgggtta
     4261 atttttgaaa atcaattgct ccttcattaa tcatattagc ttgtctttcc agataaagct
     4321 catctaaaat gtcttctaca cttt
//
LOCUS       DAONUO010000174         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14381_length_4340_cov_3.921820, whole genome shotgun sequence.
ACCESSION   DAONUO010000174 DAONUO010000000
VERSION     DAONUO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14381_length_4340_cov_3.921820"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PK768_06895"
     CDS             complement(<1..806)
                     /locus_tag="PK768_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HQA60733.1"
                     /translation="MNLNEIKQRAREKMKGYCRVCRVCNGVACAGEVPGMGGVGTGSS
                     FTENVNALANVKLNLRTLHDAKDPDISTSILGIKLSMPILAAPITGSEYNMGGAVPEA
                     EYVRMVMTGSQNAGTIGMSGDGGNPVFYDSGLEAIKGVNGHGIPIIKPRENHIVLEMA
                     KRAEEAGAPAVGMDIDGAGLVTMALMGQPVGPKNLQELKEIISGTELPFILKGIMTLD
                     EAKLALEAGASAIVVSNHGGRILDGTPGVAEVLPKIARELKGAITILADGG"
     gene            960..2039
                     /locus_tag="PK768_06900"
     CDS             960..2039
                     /locus_tag="PK768_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60734.1"
                     /translation="MHSTKGLYIAIAFIFILNTFPSHLMLGAQIDDVEQRLIEEILTL
                     DAKILALRNEIEKLSLENAELRKQLELKQEELSSLNVSFENRQTELSRWIVFAYKGGV
                     GNMLSVLVGAENLGDFFRRFDNIMFFLEYYNNVIIETRALIAQREQEERNIMEKQREI
                     EALERQAKLALEKITQTIEEKQEELRRARLILKDTTFLEEISEDWQKSLPSLDYLLKN
                     LSSLPWSSLSPDDIRVNYFAMTARAEFYDTSVTKTLLSKDENLKNVYFTFNDEGITVT
                     EKKPDSDTPVYSITCTMELTDEHKIKFTPIRLEFSGVTLPAKVIEELMADYDMVFTPP
                     PLPYDLKITSIKTEDGKLIMYFKKS"
     gene            2164..3132
                     /locus_tag="PK768_06905"
     CDS             2164..3132
                     /locus_tag="PK768_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQA60735.1"
                     /translation="MHFIKKLNTVVFLLVFLLFFSIKAEAQVITVQPGDTLNSLSRIT
                     GVPLDKIRQNNSLFSDYIAAGQVLLIPETYTVKAGDTLYLISQNFGVSFWELKSLNGL
                     YSDEIWPGQTLYVPQKSPYRQISVNWGDTLYQISQNYGVSIDDLKTVNGLWGRELYAG
                     TKLMIPSGTSSKTNSSQDLPSRGYINRGQYIKYGSGVYHTQEERMLLARLIEAEAEGE
                     PYMGKVAVGAVVVNRVLSDKFPNTIKDVIYHVDEIGAYQFEPVLDGRLFTVTTSSDSF
                     RAADEALGGLDPTGGALFFFNPYKITNKWLLSKPVICQIGDHVFTK"
     gene            complement(3170..>4340)
                     /locus_tag="PK768_06910"
     CDS             complement(3170..>4340)
                     /locus_tag="PK768_06910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQA60736.1"
                     /translation="HRRKDGSTFPVEVNSVGGVLEGKQVLISICRDITERKGYEEELK
                     RLSSFDHLTGLYNRMMFDEKLKSLKKDPPYPFALVICDIDGLKMVNDTFGHDAGDRLI
                     KAYAKIIKKAVREQDFAARIAGDEFAIIMPYTDKNTAETIIEKMRKATERTTEHGIPF
                     PLQVSMGFAIVDEENSNIKEAQKTAENNMYREKLHHGRSKKSSIIKTLVKMLEARDII
                     TQGHSQRTQELAVKLAKMLNMNQRQIDDIKLLAMFHDIGKVGVPDKILFKKGPLTHEE
                     RLRMQRHTEIGHRIAVSSPELAHISDWILMHHEWWDGNGYPLGIKGEEIPIECRILAI
                     VDAYDAMTKSRPYRPAMSHVEAVAELIRCAGTQFDPYLVDLFVKVLEEEDEVETA"
BASE COUNT         1153 a         1009 c          938 g         1240 t
ORIGIN      
        1 ccaccatccg ctaaaatagt gatcgcgccc ttaagttccc gggcaatctt gggcaggacc
       61 tctgctacac ccggcgtgcc gtctaagatg cgcccgccgt gatttgacac cactatagcc
      121 gatgctccgg cttccagggc cagcttggct tcatcaaggg tcataattcc ctttaatata
      181 aatgggagct ctgtgccgga aatgatttct tttagctcct gcaaattttt tggtccaacg
      241 ggctgtccca tcaaggccat cgtaacaaga cccgctccat caatatccat gcctacagca
      301 ggggcccccg cttcctcggc cctttttgcc atttccaaaa ctatatgatt ttcccgaggc
      361 tttatgatgg ggataccgtg gccatttacc ccttttattg cctcaagtcc cgaatcataa
      421 aatacgggat tgccgccgtc accgctcatt ccaatagtcc ctgcattttg gctgccggtc
      481 attaccatgc gcacgtactc ggcctcgggc actgcgccac ccatgttata ctcggaaccc
      541 gtaatcggtg ctgcaagtat cggcatcgag agctttatgc cgagaatact cgttgaaata
      601 tccggatcct tggcgtcgtg aagggtcctc aagttcaact taacatttgc cagggcattg
      661 acattttcag taaaggaaga acctgttcca actcctccca tccccggcac ttctccggca
      721 catgctacgc cgttacatac cctgcacact cggcagtaac ctttcatctt ttcccgtgcc
      781 ctttgcttta tttcattcag attcaaaaga tttcacccct ttcaaaaacc tatatttaaa
      841 ttatacacca tatagttaca atctcccaaa taatgaagct tatgacttta gaggatattt
      901 tagctttttg tagaataaac tatttataaa catgcttgtc ctttttgcgg gggaataaaa
      961 tgcattcaac taaggggctt tatatagcaa tagcttttat ttttatttta aatacttttc
     1021 cttctcactt gatgcttggt gcccagatcg acgatgtgga gcagcggtta atcgaagaga
     1081 tactgacctt agatgcaaaa atcttagctc tcagaaatga aattgaaaaa ctttctttag
     1141 agaatgcaga gctaagaaag caactggagt taaagcagga ggagctttcg tctttaaatg
     1201 tcagttttga aaaccgccaa actgagcttt cccgctggat tgtctttgcc tataaaggcg
     1261 gcgtcggcaa tatgctatcc gtgctggtag gcgcggagaa tttgggagat tttttccgcc
     1321 gtttcgacaa catcatgttc ttcctggaat actataataa tgtaataatc gaaaccagag
     1381 cccttattgc tcaaagagaa caggaggaac gaaatatcat ggaaaaacag cgggaaattg
     1441 aggctttaga gagacaggca aaattggccc ttgaaaaaat aactcaaact atagaagaaa
     1501 agcaagagga attaaggcgt gccaggctca tattaaaaga taccacattt ttggaagaaa
     1561 taagtgagga ttggcagaaa tccctgcctt cccttgacta tcttctgaaa aacttatcgt
     1621 cactgccctg gtcaagttta agccctgatg atattagggt aaactatttt gccatgacag
     1681 cccgggcaga attttacgat acaagtgtca caaaaacttt gctgtcaaaa gacgaaaact
     1741 tgaagaacgt atacttcacc tttaacgatg aaggcattac ggttaccgag aaaaagccgg
     1801 atagcgatac gccggtttat tccataactt gcaccatgga acttaccgat gagcataaaa
     1861 taaaatttac ccctataaga cttgaattta gcggtgtgac actgccggcc aaagtgattg
     1921 aagagctgat ggctgattac gacatggtat ttactccgcc gcctttgccc tatgatttga
     1981 aaataacttc tataaaaact gaagatggca aactcatcat gtattttaaa aaatcctgat
     2041 ttgatactgc cgggggaatt ccccggtatc tttttttccg gattttccct tggtgaattt
     2101 tacttgatga aatagacggt ggacctatag tataatcatg ccaaggaaag gggatggtat
     2161 ttggtgcatt ttatcaaaaa actaaacacg gttgttttcc tgcttgtctt cctgcttttc
     2221 ttctccatca aggctgaagc ccaggtaatt actgttcagc ccggggacac cttaaattcc
     2281 ttaagtcgga ttaccggtgt tccgctagat aagataaggc agaacaattc cttgttttca
     2341 gattacatag cggctggaca agttctgctg attccggaaa catacacggt aaaagccggc
     2401 gatactcttt atctcattag ccaaaacttt ggtgtaagct tttgggaatt gaaaagctta
     2461 aacggtcttt actctgacga aatctggccg ggacagacac tatacgttcc ccaaaaaagt
     2521 ccttatcggc agataagtgt aaactgggga gacactcttt accaaatttc acaaaattac
     2581 ggggtgtcca tagatgattt gaagaccgtt aacgggcttt ggggaaggga gctatatgcg
     2641 ggaactaagc tcatgattcc ttcaggaaca tcctcaaaga caaacagctc tcaagatttg
     2701 ccgtcaaggg gctatataaa tcggggacag tacataaaat acggcagcgg agtatatcac
     2761 acccaggagg aaaggatgct cttggcacgg ctgattgaag cagaagcaga aggagaacct
     2821 tacatgggaa aggttgcagt tggagcggtg gttgtaaaca gggtacttag cgataagttt
     2881 ccaaatacga tcaaagatgt tatatatcac gtagacgaaa taggcgcata ccagtttgag
     2941 cccgtccttg acggaaggct gtttaccgta actaccagca gtgattcatt tagggctgcc
     3001 gatgaggctc ttgggggctt ggaccccaca ggaggtgcct tgtttttctt taacccttac
     3061 aagataacaa ataaatggtt gctgtcaaaa cctgtaatct gccaaatcgg cgaccacgtt
     3121 tttacgaagt aaagtgaatg aaaaactccg cacaaggcgg agtttctttt tatgctgttt
     3181 ctacttcatc ttcctcttcc aaaactttga cgaataaatc cacaaggtaa ggatcgaact
     3241 gggtcccggc gcatcttatg agttccgcta cggcctctac atgactcatg gcagggcggt
     3301 aaggcctgct cttcgtcatt gcgtcgtaag cgtcaactat ggccagtatg cggcattcga
     3361 tcgggatttc ttcgcccttt atgcccaggg ggtatccgtt gccgtcccac cattcgtggt
     3421 gcatcagtat ccagtctgaa atatgggcaa gttctggaga tgacactgct atgcgatggc
     3481 cgatttccgt atggcgctgc attctcaatc tctcttcatg ggtcaggggg cctttcttaa
     3541 acaggatttt gtcaggcact cccactttgc ctatatcgtg gaacatggca agaagtttaa
     3601 tgtcgtcaat ttgccgctga ttcatgttta acatcttggc cagctttact gcaagttctt
     3661 gagtcctctg ggaatggccc tgggtgatga tgtcccgggc ctccagcatc tttaccaggg
     3721 tctttatgat gctgctcttt ttgctcctac catgatgaag tttttcccgg tacatattgt
     3781 tttctgcggt tttctgtgct tccttgatat tgctgttttc ctcatcaact atggcaaagc
     3841 ccatggaaac ctgcagcgga aatggaatgc catgttcagt agttctttca gtggccttgc
     3901 gcatcttctc aataatcgtt tctgccgtat tcttgtccgt atagggcatg attatagcaa
     3961 attcatctcc ggcaattctc gcagcaaaat cctgctctcg cactgctttt tttataatct
     4021 tggcatacgc ctttatcaag cgatctcctg catcatggcc gaaagtgtca ttgaccatct
     4081 taagaccgtc tatgtcgcag attaccaagg caaaaggata aggcgggtct tttttcagtg
     4141 attttagctt ttcatcaaac atcattctat tgtacagccc cgtgagatga tcgaaactgc
     4201 tcaatcgctt gagctcttcc tcatatccct tgcgttcggt tatatcacgg caaatactta
     4261 taagtacctg cttgccctcc agcacgccgc ccacggaatt tacttcaacg ggaaaagtgc
     4321 tgccgtcttt gcgccgatgg
//
LOCUS       DAONUO010000175         4283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14612_length_4283_cov_4.384106, whole genome shotgun sequence.
ACCESSION   DAONUO010000175 DAONUO010000000
VERSION     DAONUO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14612_length_4283_cov_4.384106"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..758
                     /locus_tag="PK768_06915"
     CDS             18..758
                     /locus_tag="PK768_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HQA60737.1"
                     /translation="MSILQMILVSAWAGISGNYFMDYLGFARPVVSGLVVGLILGDVQ
                     TGVILGATIEAMFLGIFTVGAALAPDYNLAGVVGVALGIASGYGVEAAVALALPVALF
                     GQFLMMSIVYQGNLVFLHMADRYAEEGKINKIETAYLSGALLWFVKGFIPTFLALYFG
                     ASAVEGLFNALPVWLVDGFSIVGGILPAVGFAMLLNVIGASGEIWALFAAGFVLVSYL
                     HLDILALAVLALVIAYLYVSLEPTKEVR"
     gene            759..1589
                     /locus_tag="PK768_06920"
     CDS             759..1589
                     /locus_tag="PK768_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HQA60738.1"
                     /translation="MAENQERKKISQKALTKAAWRVLLFENSINFERMQSFSFTYAMI
                     PILKDLYKNTEDIIQGLKRHLQFFNSNVLSASFIMGAAASMEEQKANGEPISDEAINS
                     VKAGLMGPGAGIGDALFWGTFVPLVGGLAAAMAAKGNAFAPILHQIIRVGVWIAVIQF
                     GVKFGYREGLALLEKAGQAGIQKITRGATILAATVIGGLVAMLVNAKTELVITSGEVG
                     INLQTDVLDPIAPNLIPLMLFLLTYYLISKRKWSPIAVIGFVFVLGIGASFLNILAVP
                     "
     gene            1610..2656
                     /locus_tag="PK768_06925"
     CDS             1610..2656
                     /locus_tag="PK768_06925"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQA60739.1"
                     /translation="MRAAVYFGPGKVELDEFREPILEDGDIKIKVELCGVCGSDIKTY
                     VRGTPYTIPPAVLGHEVVGTVVESRSPKWQEGDRVAVAHYIPCGVCPLCLDGKGTLCP
                     ELFDRRIDPGGFAERMRIPRDLAERATFKIRDDVSFKQAVLAEPLACCIHAARQLSIS
                     PKSSVLIIGDGPMGLLHTQTAKAFGAGKVILSGMTPHRLQRAKQLADYVIDANTCDVV
                     SKVKEITGEGPNHIIVAVPSIEAAKQALDMACLGGEVLLFGGFPKNQSLSLDPNRIHY
                     DEVKLLGTVGSLPEEFHMALTLLESGRVNADLFITHRYSLDKVAYALEQGTKQVGIKA
                     IVDLSAPEGTAEEL"
     gene            2668..3513
                     /locus_tag="PK768_06930"
     CDS             2668..3513
                     /locus_tag="PK768_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HQA60740.1"
                     /translation="MLLSMTEMLKKARAGKYAIPQPDFVNIQMAAMYLEAAKQSASPI
                     ILGYGEEYLNTTEGVDLRHLVRLIEVLAKEYPIPVALHLDHGSSFEACAEAIAAGFTS
                     VMFDGSSLPFEQNVAITRKVVEMAHPAGVSVEAELGYVGTAKDTDDDSRLTDPEKARI
                     FVKATGVDALAVSIGTVHGEYKGKPNIRLDLLSEISAKVDVPLVLHGASGTGHDTLRE
                     CVKRGICKVNIYTDLVKVMIGSLKKDIQQQKPNIVTVIDNMHNEVVQELKNYIKTLGA
                     AGRCN"
     gene            complement(3679..4086)
                     /locus_tag="PK768_06935"
     CDS             complement(3679..4086)
                     /locus_tag="PK768_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQA60741.1"
                     /translation="MSISLLLKQHVQISEILQELMSIPQEGLAEKAFEISKKIGQLAG
                     VLKFHLQSEDKFLYPKLMKHESSHIRDTAFAFTDEMGNLGEKFEAFKATYIQPKNIKA
                     APEKFKQDLDSIATALKIRIEREDRKLYPLLDR"
BASE COUNT         1175 a          810 c         1034 g         1264 t
ORIGIN      
        1 cgagaggagg acaataattg agcattctgc aaatgatttt agtatctgcc tgggcgggaa
       61 tatccggtaa ttattttatg gactatttag gctttgccag gccggtagta tcaggtttgg
      121 tcgtaggtct gattttaggt gatgtgcaga ccggtgttat cctaggtgca actattgagg
      181 ccatgttttt aggtattttt actgtaggag cggccttagc ccctgattac aacttggcag
      241 gtgttgtggg agtagccttg ggcatagctt cgggttacgg tgttgaggct gcggttgcct
      301 tggctttacc ggtagcattg tttggccagt ttttgatgat gagtattgtt tatcagggca
      361 atttagtatt tctccatatg gctgatcgtt atgccgagga aggtaaaatc aacaaaattg
      421 aaactgccta tctcagcggt gcactgctat ggtttgtcaa aggttttatt cccacctttt
      481 tagctttata tttcggagca tctgcggtag aaggattgtt taatgctctt cctgtttggt
      541 tagtagacgg attttccatt gttggcggca tactgccggc tgttggattt gccatgctcc
      601 tcaatgttat tggtgcttcc ggggaaattt gggcattgtt tgccgcaggt ttcgttttag
      661 tgagttattt gcacctagat atacttgcat tggcggtttt ggctctggtt attgcttact
      721 tgtatgtctc cctagaacct accaaggagg tcagataaat ggctgaaaat caagaaagga
      781 aaaagataag tcagaaggca ttgaccaaag ctgcttggag agtgctgctc tttgaaaact
      841 caataaactt tgagcgtatg caatcatttt cgtttactta tgctatgatt cccattttaa
      901 aggacttgta taaaaacaca gaggacataa tacaaggtct taagcggcac cttcagtttt
      961 ttaacagcaa tgtgctatcc gcatcattta taatgggtgc agccgcttca atggaagaac
     1021 aaaaagccaa cggagagcct atcagtgatg aagccataaa tagcgttaaa gctggcctta
     1081 tggggccggg agccggcatc ggagatgcat tgttttgggg gacatttgtt ccgctggtag
     1141 gaggacttgc ggccgctatg gctgctaaag gaaatgcttt tgcacccata ttgcaccaaa
     1201 tcattcgcgt gggtgtatgg attgccgtta ttcaattcgg cgtgaaattt ggctatagag
     1261 aaggattggc gttactggaa aaagcagggc aggcaggaat ccaaaaaata actcgaggcg
     1321 ccacgatatt agcggcaaca gtcatcggag gtttggtggc gatgctggtt aatgctaaaa
     1381 cggaacttgt gataacttca ggtgaagtgg gaataaatct gcagactgat gtactggacc
     1441 ccatagcgcc caacctgatt cctcttatgc tatttctatt aacatactat ctgataagca
     1501 agcgcaagtg gtcgcccatt gctgtgatag gttttgtatt tgttcttgga atcggtgctt
     1561 cattcctgaa cattttagct gttccgtaaa tcaaaggagg taattattga tgcgtgctgc
     1621 agtatatttc gggccaggaa aagttgagct tgatgaattt cgagaaccaa ttttggaaga
     1681 tggagatatt aagataaagg tggaattgtg cggggtttgc ggttctgaca taaaaaccta
     1741 tgtgagaggc accccttaca caattccccc tgcagtactc ggccatgaag tagtgggaac
     1801 ggtagtcgaa agccgttccc ctaaatggca ggaaggtgac agagtagctg tagctcatta
     1861 tattccatgt ggtgtttgtc ccctctgcct tgatggaaaa gggaccttat gtccagagct
     1921 ttttgaccgt agaattgatc ccggaggatt tgccgaaagg atgcgaatcc ctagagattt
     1981 agctgaacgt gctaccttca aaatccgaga cgatgtgagc tttaaacaag cggttttagc
     2041 agaaccttta gcctgttgta tacacgctgc aagacagctt tcgatttcac caaaaagcag
     2101 tgtgcttata attggggacg gccctatggg cttgctgcat acccaaacgg caaaagcttt
     2161 cggtgccggg aaggtaatat tgtccggaat gactcctcac aggcttcaaa gggctaagca
     2221 gcttgcagat tatgttatag atgcaaatac ttgcgatgta gtttcgaaag taaaagaaat
     2281 taccggggag gggcctaatc acattatagt ggcggtgcca tcaatagagg cagccaagca
     2341 agcccttgat atggcttgct tgggtggtga ggttctgctt ttcggaggtt ttcccaaaaa
     2401 tcaaagccta tcattggatc caaatagaat tcactatgat gaagtaaagc tcttaggcac
     2461 tgtaggttcc ttgcctgaag aatttcacat ggcattgaca ctgctggaat ctggcagagt
     2521 aaatgcagac ctttttataa ctcacaggta cagtttggat aaagttgcct atgcgctgga
     2581 gcagggcaca aagcaagtag gaataaaagc tattgttgat ttatctgctc cggaaggaac
     2641 cgcagaagaa ttataggggg aaaacagatg ttactttcaa tgacggaaat gctcaaaaag
     2701 gctcgtgcgg gtaagtatgc cattccacaa ccggattttg ttaatattca aatggctgcc
     2761 atgtatctgg aagcagctaa gcaatccgcc tcacccatta ttctcggata tggagaggag
     2821 tatctgaata ctacggaggg tgtggattta cgacatctgg taagactcat tgaagtatta
     2881 gcaaaggaat atccaattcc tgtggccctg cacctcgacc acggcagctc ttttgaagca
     2941 tgtgcagaag ctattgccgc tggttttact tcggttatgt ttgacggctc atcattgccc
     3001 ttcgaacaaa atgtggctat taccagaaaa gttgtggaaa tggcacatcc agcaggggta
     3061 agtgtagaag cggaactggg ctatgtggga acagcaaagg atacggacga tgattcccgt
     3121 ttaactgatc ctgaaaaggc ccgtatcttt gtaaaagcga ccggggtgga tgcactggcg
     3181 gtttcaatcg ggactgttca cggtgagtat aaagggaagc ccaatatccg tctggattta
     3241 ttgtcggaaa taagtgcaaa ggtagatgtt cctttagtgc ttcacggagc ttcaggtacc
     3301 ggccatgata ctcttcgtga atgtgtaaaa cgcggcattt gcaaagttaa tatatatact
     3361 gacttagtaa aagtaatgat tggttcactg aaaaaagata tacagcaaca aaagcccaac
     3421 atagtcacag ttatagataa tatgcataac gaggtagtgc aagaattaaa aaattatatt
     3481 aaaactttag gagcagcagg caggtgtaac taattagaca tcacaaaatt taaaaaaccg
     3541 cacagctttg atatgacacc gctcatagtc tggtttgaaa taaaagaatc ccccgacaca
     3601 ttctgtaata tgttgtcgga ggggttcttg ttcttatgtg aaatgaagat gttgtcttct
     3661 tctgaaggtc aatttgcatt atctatctaa aagcggataa agctttctat cttccctttc
     3721 aatcctaatt ttcagggcgg tagctatact atccaagtct tgtttaaatt tttcaggagc
     3781 tgcctttatg tttttcggct gtatgtacgt tgccttaaaa gcttcaaact tttcacctag
     3841 gtttcccatt tcatccgtga aggcaaaagc tgtatcgcga atgtggctgc tttcatgctt
     3901 catcaatttg ggatataaga acttgtcttc agattgcaaa tgaaatttga gcacacctgc
     3961 aagctgccct attttctttg aaatttcaaa ggccttttct gccaaaccct cttgtggaat
     4021 gctcatcagt tcctgtaaga tttcgctgat ctgtacatgc tgttttaaaa gaagcgagat
     4081 gctcatacat acaccacctt tttccatttt tccctatcat actaaagacc cacgtacatt
     4141 tgtatgatat atatcacaaa gcgaattaag atactgcata aatcgagggg ataaggagga
     4201 aattattgct gaggtgatgt ctttgaaaaa gaattacggt cttttcacaa tgatgatttt
     4261 attactttcc ttggcaattt tcg
//
LOCUS       DAONUO010000176         4237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14794_length_4237_cov_3.727642, whole genome shotgun sequence.
ACCESSION   DAONUO010000176 DAONUO010000000
VERSION     DAONUO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14794_length_4237_cov_3.727642"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /gene="map"
                     /locus_tag="PK768_06940"
     CDS             complement(<1..744)
                     /gene="map"
                     /locus_tag="PK768_06940"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQA60742.1"
                     /translation="MIVLKSPREIELMRRAGEITALALEEIEKHVKPGITTIELDKIA
                     EELILSHDATPAFKGYRGFPASICASINEEVVHGIPGLRRLQDGDIISIDIGAVFQGY
                     YGDAARTFPVGQVSDDALKLIEVTKQSFFEGIAFAKQGYRLSDISHAIQSYVEGHNYS
                     VVRDYVGHGIGQNMHEDPQIPNFGPPGKGPRLRAGMTLAIEPMVNLGEFEVYTLKNNW
                     TVVTKDGSLSAHYENTVAITDGEPEVLTMM"
     gene            complement(741..1394)
                     /locus_tag="PK768_06945"
     CDS             complement(741..1394)
                     /locus_tag="PK768_06945"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQA60743.1"
                     /translation="MRIVLLGPPGAGKGTQAKKISEEYEICHISTGELLRKAVQENTP
                     LGKKAGEYLDRGLLVPDEIIDGMVKDRLKMLDCERGFLLDGFPRTLGQAESLDGFLKE
                     EKLSLDIVIKIQVEQAKLIERFTGRRTCKNCGASFHIKFSPPKKAGVCDNCGGELVIR
                     KDDEEETVKKRLQVYETQTTPLIDYYDRQKLLVTVDGSKAIDDVFEDIKSYLEGVKS"
     gene            complement(1407..2660)
                     /gene="secY"
                     /locus_tag="PK768_06950"
     CDS             complement(1407..2660)
                     /gene="secY"
                     /locus_tag="PK768_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQA60744.1"
                     /translation="MLEALRNAWKIPDLRRRIKYTALMLLLFRMGTFIPVPRMDPAAV
                     RSIVERGALLGFFDIIAGGAFSQFSVFAMSITPYINASIIMQLLTIVIPRLEELAKEG
                     EAGRKIIAQYTRYGTVILGLIQATGLSIGFKSAMKPGFLSNIVVIVSLTAGTAFLMWL
                     GEQITEKGIGNGISLLIFTGIVSRIPASIIQTYALVRVGTINILLLAVIAILGVAVIV
                     GVIAVQEAERRLPVQYAKRIVGRRVYGGQSTHMPIKLNSAGVIPVIFAMSILMFPSTL
                     AGFFPSSKFMTSIAEFLQPSGWLYAVLYAMLIVFFTYFYTAVTFNPVDVANNMKKYGG
                     FIPGIRPGRPTAEYISRIVNRITLVGALFLAAIALLPYFMTAISGLNISFGGTALLIV
                     VGVALETMKSIEAQMLMRHYQGFLK"
     gene            complement(2662..3102)
                     /gene="rplO"
                     /locus_tag="PK768_06955"
     CDS             complement(2662..3102)
                     /gene="rplO"
                     /locus_tag="PK768_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQA60745.1"
                     /translation="MRLHDLAPAEGSSKKAKRVGRGIGSGRGKTSTRGHKGQNARSGG
                     GVRPGFEGGQMPLYRRIPKRGFTNIFKKQFVAINVDELNRFDEDTKVTPELLVEKGII
                     KKIKDGVKILGDGQLDVRLHVVAHAFSETAKEKIEAAGGKTEVI"
     gene            complement(3124..3609)
                     /gene="rpsE"
                     /locus_tag="PK768_06960"
     CDS             complement(3124..3609)
                     /gene="rpsE"
                     /locus_tag="PK768_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQA60746.1"
                     /translation="MEESEFVERVVSIKRVAKVVKGGKNFRFSVLVVVGNKKGQVGVG
                     NGKAQEIPEAIRKGIEDAKKNLIEVPILDGTIPHESLGIFGAGRVLLKPAPEGTGVIA
                     GGPVRAVVELAGITDIVSKSLGSANARNMVNATIEALKQLKRAEEVAKLRGKTVEEIL
                     G"
     gene            complement(3646..4011)
                     /gene="rplR"
                     /locus_tag="PK768_06965"
     CDS             complement(3646..4011)
                     /gene="rplR"
                     /locus_tag="PK768_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQA60747.1"
                     /translation="MLKKESRNVARKRRHLRVRKKVFGTEERPRLCVYKSEKHIYAQI
                     INDEQGHTLVAASTLDPAIRDKVTGANIESAKLVGDLIARKALEKGIRAVTFDRSGYI
                     YHGRVKALADAAREAGLDF"
     gene            complement(4034..>4237)
                     /gene="rplF"
                     /locus_tag="PK768_06970"
     CDS             complement(4034..>4237)
                     /gene="rplF"
                     /locus_tag="PK768_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQA60748.1"
                     /translation="PVEFEPPEGIEFEVPAPNRIIVKGIDKELVGQIAANIRAVREPE
                     PYKGKGIRYEDEVVRRKEGKTGA"
BASE COUNT          972 a         1159 c          851 g         1255 t
ORIGIN      
        1 catcatagtc agtacttccg gctcgccatc cgttatggca actgtgtttt catagtgagc
       61 cgaaagactg ccatccttcg taacaaccgt ccagttgttt ttcagcgtgt aaacctcaaa
      121 ttcccccaga ttcaccatgg gctcaatggc cagtgtcatc ccggccctaa gtctcgggcc
      181 ttttcctggt ggaccgaaat tcggtatctg cggatcttca tgcatatttt gcccgatacc
      241 gtgacctacg tagtccctca ctaccgaata gttgtgccct tctacatagc tttgaatggc
      301 atgggatatg tcagagagcc tgtatccctg ttttgcaaaa gctatgcctt caaaaaagct
      361 ctgcttagta acttcaatga gtttgagggc atcgtcactt acctggccta ccgggaatgt
      421 tcgagctgca tcaccgtagt agccttgaaa tacagcacct atgtctatac taataatatc
      481 accatcttgt agccgtctta atccaggaat cccatgtact acctcttcat ttatcgaagc
      541 acatatggat gccggaaaac ccctgtagcc tttgaaggct ggagttgcat cgtggctcaa
      601 gatgagctcc tcggcaattt tatcaagttc gatagttgtt attccgggct ttacatgctt
      661 ttctatctct tccagcgcaa gtgccgtaat ttcaccggct cttcgcataa gctcgatttc
      721 ccttggagat tttagaacaa tcatgatttt acaccttcaa gataactttt tatatcttca
      781 aagacatcgt caatggcctt tgaaccgtca acggttacca acagcttttg gcgatcgtaa
      841 taatcgataa gtggggttgt ttgagtttca tacacctgca atcgcttttt tacggtctct
      901 tcctcatcat ccttgcgaat cacgagctct cctccacagt tgtcgcatac cccggctttt
      961 ttcgggggac tgaactttat gtggaagctc gctccgcaat ttttgcatgt ccgcctgccg
     1021 gtaaaccttt cgatgagctt tgcctgctcc acttgaatct tgattacaat gtccaggctg
     1081 agcttttctt cctttaaaaa tccatcgaga ctttccgcct gccctagcgt cctcggaaaa
     1141 ccgtccagca aaaaacccct ctcgcagtcc agcattttaa gcctgtcctt gaccatgcca
     1201 tctattattt catccggaac caaaagacct ctatcaaggt attcgccggc ctttttgccg
     1261 agcggagtat tttcctggac tgcctttctc aatagttcac ccgtcgaaat atggcaaatc
     1321 tcatattcct ccgaaatctt ctttgcttgg gtacccttgc ctgctccggg agggcccagc
     1381 aagacaatcc tcaattcaaa tcactcctac tttaagaatc cctggtaatg tctcatgagc
     1441 atctgcgctt cgatggattt catggtctct aaagctacac caactacgat caagagagcg
     1501 gtgcctccaa aggatatatt taatccggat atggcagtca tgaaataggg cagtagggca
     1561 atggctgcca aaaatagggc gcctaccagt gttatcctgt tcactatgcg gctgatgtat
     1621 tcggccgtcg gtctacccgg gcgaatacct gggataaagc cgccgtactt tttcatattg
     1681 tttgctacat ctacaggatt gaaggttacg gccgtgtaaa aataggtgaa aaacacgata
     1741 agcatggcgt ataggaccgc atacagccat ccgctgggct gcaagaactc agctatactc
     1801 gtcatgaact tgctgcttgg gaaaaagccg gccaacgtac tgggaaacat caggattgac
     1861 attgcgaata ttacagggat aacgcctgcc gaattcagct taatcggcat gtgagtcgat
     1921 tgaccgccat atactctgcg tcccactatt cgcttggcat actgcactgg cagtctgcgt
     1981 tccgcttcct gcacggcaat aactcccacg attaccgcta ctcctaaaat cgcaataaca
     2041 gccaacaaga gtatattgat agtgcccact cttactaagg cataggtctg aatgatgcta
     2101 gccggaattc tggacacgat accggtaaag attagcaggg aaatgccgtt gcctatgcct
     2161 ttttcggtaa tttgctcacc cagccacatc aaaaacgcag tacctgcggt aaggctgact
     2221 attaccacga tattgctcaa aaaccctggc ttcatggcgc tcttaaagcc tattgaaagg
     2281 cccgtcgcct ggatcagccc taaaatgacc gtcccgtaac gggtgtactg ggcaattatc
     2341 ttgcgccccg cctctccttc cttggctagc tcttctagcc ggggaatgac tatggtcaag
     2401 agctgcatga taatagaagc attaatgtaa ggcgtgatac tcatggcaaa gaccgaaaac
     2461 tggctaaatg caccaccggc aatgatgtca aaaaacccga gcagtgctcc tctttcaacg
     2521 attgaccgca cagcagcagg gtccatccta ggaaccggta taaaggtccc catcctgaac
     2581 agcaataaca tcaacgcagt atacttaatc cgccttctta aatccggtat tttccaggca
     2641 tttctcaggg cttccagcat cttaaatcac ctctgtcttt ccaccggctg cttctatctt
     2701 ctctttagcc gtttctgaga aagcgtgggc tactacgtgg agtcttacat cgagctggcc
     2761 gtcaccaagg atttttacac cgtcctttat ttttttgatt atgccttttt caaccagcag
     2821 ctccggagta acttttgtat cttcatcgaa tctgtttaac tcatccacgt ttattgctac
     2881 aaattgtttt ttgaaaatgt ttgtgaatcc tctcttggga atgcgtctgt acaggggcat
     2941 ctggccgcct tcaaatccgg gtcgcactcc gccgccgctc cgggcattct ggcctttgtg
     3001 acccctggta gaggttttgc cgcgtcccga acctattcct ctgccaactc ttttagcttt
     3061 cttgcttgaa ccttcagccg gagcaagatc atgcaatctc attgcttgca cctcctcttt
     3121 gctttatcct aatatttcct ctacagtctt gccgcgaagt tttgccactt cttcagcccg
     3181 cttcaattgt ttcagtgctt ctatagttgc atttaccatg tttctggcat tggcagaacc
     3241 taaagacttg ctcacgatgt ccgtgattcc tgccagttcc acaactgctc tcacaggacc
     3301 ccctgcgata actcccgttc cctcgggggc cggctttaat aagactcttc ctgctccaaa
     3361 aatcccgaga ctctcatggg ggatagttcc gtccagtatt gggacctcaa ttaaattctt
     3421 tttggcatcc tcgatgcctt tgcgaatagc ttcgggtatc tcctgggcct tgccgttacc
     3481 tactcctacc tggccttttt tatttccaac cacgactaaa acggaaaacc taaagttttt
     3541 gcccccttta acaaccttag ctactctttt tatggaaaca accctttcga caaattcact
     3601 ttcttccacg tttctattcc tgtgctgcaa agttgtacct cccttctaaa agtctaatcc
     3661 ggcttctctg gctgcgtctg ccagcgcttt tacccggccg tgatatatat aaccgctgcg
     3721 gtcaaaggtc accgcacgaa ttcccttctc caaagctttt cttgcaataa ggtcgcctac
     3781 cagttttgcc gactcaatat tggcaccggt caccttatct cttatagcag ggtcaagggt
     3841 ggatgctgca accagtgtgt gtccctgttc gtcgtttatt atttgagcgt agatgtgctt
     3901 ttcgctcttg tagacacaca ggcgcggtct ttcttctgtg ccaaagacct tcttgcgaac
     3961 cctaagatgc cttctttttc gggctacgtt gcgcgattcc ttttttagca tatgtttccc
     4021 tccctatagc ttattacgct ccagtcttac cttctttgcg acgtacaacc tcgtcttcgt
     4081 acctgatacc cttgccttta tacggttcgg gttctctaac cgctcttata ttcgccgcaa
     4141 tttggcctac cagttcctta tcaatacctt ttacgataat cctgttagga gcgggcactt
     4201 caaactctat cccttctggg ggttcaaatt caacagg
//
LOCUS       DAONUO010000177         4218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14877_length_4218_cov_3.335335, whole genome shotgun sequence.
ACCESSION   DAONUO010000177 DAONUO010000000
VERSION     DAONUO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14877_length_4218_cov_3.335335"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PK768_06975"
     CDS             <1..1312
                     /locus_tag="PK768_06975"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQA60749.1"
                     /translation="LRNQDVFLDMLTDSGVNAMSDRQEAAMLLADDSYAGSETFYRLE
                     SKLKEIFGMDFFLPAHQGRACEHIIAKTLVKPGQIVPMNYHFTTTKSHIVLCGGTVEE
                     LLIDEGLDANNKHPFKGNMDIDKLEAVIQEHGPDKIAFVRMEAGTNLIGGQPFSLENL
                     AEVRKVCDKYGIILVMDASLLQDNLHFIKSREEQCKDMTIREITRKMASLCDIIYFSA
                     RKLGCARGGGLCFRDEKLYKLMMEFIPLYEGFLTYGGMSVREMEAIAVGLDETMDEDV
                     ISQGPQFIDFMCNELIKHGVPVVTPPGGLGCHINAMEFVDHIPQSQYPAGALAAALYI
                     AGGIRGMERGTLSEQRNPDGSECFANMELLRLAMPRRVFTLSQVKYAVDRIVWLYENR
                     RLIGGLEFVYEPSILRFFFGRLKPVSDWQEKLLKKFREDFGDSL"
     gene            complement(1350..2906)
                     /locus_tag="PK768_06980"
     CDS             complement(1350..2906)
                     /locus_tag="PK768_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HQA60750.1"
                     /translation="MSIQILIAMVLYIGAVIAIGLNYAKTASESTENFLIGGRSLGPW
                     VTALAAEASDMSGWLLMGLPGVAYWVGLSDAFWTGIGLLVGTYLNWLFVAKRIRAYSE
                     IAGNSITIPDFISKRFKEENKVLMTLAALFILVFFTVYAASCFVTVGKLFSTLFNFKY
                     VYMMVAGALFVIIYTFVGGFLAESASDFMQGVVMIFALVAVLVAGIGAAGGLSQVVQN
                     AKQIPGFFEFFGIASPQLADGVQQVVDGKPVFGEAGSYGWLTVISTLSWGLGYFGMPH
                     VLLKFMAIRETSELTLSRRIGTLWCAISLGASIGIGIIGRVIFPDAYLTQSAAENIFI
                     LMSTSFFVPIFAGIVMSGILAATISSADSYLIISASAFSKNIYHGLIKREASDREVLT
                     MTRIALLVISLFAIFIALDENSVIFKVVSFAWAGFGATFGPTVLFSLFWKRVSRAGAM
                     AGMLTGGITVFVWKLLVRPLGGVFDIYELLPAFVLSCVAIVVVSLLSEEPSEEVLKEF
                     ELARNMSMGL"
     gene            complement(3094..>4218)
                     /locus_tag="PK768_06985"
     CDS             complement(3094..>4218)
                     /locus_tag="PK768_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60751.1"
                     /translation="LKRIPGLELGDDPIVPIREFFESQGGKVSWDNNNWHVIVSWREK
                     SGDLTADELVIKSSELLKEANTYRMKAVNEIGLDLQGDEVLKALMGVPINMELVIEGV
                     FRNEPMSMHMKQTMKMPLEMLGVSPEEMEASGLAEGIVTETVWHDNVIYTKNPDTGQW
                     IYQDLTGMEEMIGFDSLIQMTPQQSIEMMNEAGVINVFGDDAVIEGREYYTIKNYIDA
                     ESYRSLVEQVLGNINLGSFMAAFAAQAGDGEDVSAQLEQIFDIVLNNIVFDYYIDTFI
                     NKETLLSDYMKIDLNMVIDVKQLIEAIAEVTEMDEEEKAAIPEGPMVFQINMKGDYQM
                     YDYGVELELPDLSDAISQEEYMQQIMEMMEEIEEAEALAE"
BASE COUNT         1170 a         1066 c          913 g         1069 t
ORIGIN      
        1 gctcagaaac caggatgtct ttttggacat gctgacggac agcggcgtca atgccatgag
       61 cgacaggcag gaggcagcaa tgctgctggc agatgacagc tacgctggaa gcgaaacctt
      121 ttaccggctc gaaagcaagc tgaaggaaat attcgggatg gacttcttcc tgccggcaca
      181 ccagggcagg gcatgtgagc acatcatcgc aaaaaccctg gtaaaacccg gccagatagt
      241 tccgatgaac taccacttta ccaccaccaa gtcccacata gttttgtgtg gcggaacggt
      301 ggaggagctc ttaatcgacg aaggcctgga tgccaataac aaacatccct ttaagggcaa
      361 tatggatata gacaagcttg aagctgtcat ccaagagcat ggcccggaca aaattgcctt
      421 tgtccgcatg gaagcaggga caaaccttat cggcggtcag cccttctctt tggaaaactt
      481 agctgaagtg cgcaaagtct gcgacaagta cggtatcatc ctcgtcatgg atgccagctt
      541 acttcaagac aacctgcatt ttatcaaaag ccgggaagaa caatgcaagg acatgacaat
      601 tagggaaatt acccgcaaga tggcgtccct ttgcgatatc atatattttt ccgcccgtaa
      661 actcggttgt gcaagaggcg gcggactgtg ttttagggat gaaaagctgt acaaactcat
      721 gatggaattt atacctcttt atgaaggctt tttgacatac gggggcatgt cagtcaggga
      781 aatggaagca atagccgtag gcttagatga aactatggat gaagatgtaa taagtcaagg
      841 accgcagttt atcgatttta tgtgcaatga gctaataaaa cacggggttc ccgtcgtcac
      901 tcctcccggc ggcctgggtt gccacatcaa tgccatggaa tttgtggacc acatacccca
      961 aagccaatac ccagcaggcg cactggcagc ggccctttac atcgccggcg gaattcgcgg
     1021 catggagcgc ggcacactat cggaacagag aaatcccgat ggaagcgaat gttttgcaaa
     1081 catggagctc ttgaggctcg ccatgccgag acgggtattt accctttccc aggtgaagta
     1141 tgcagttgac aggattgtat ggctgtatga aaatcgaagg ctcatcggag gcttggagtt
     1201 cgtatacgag cccagcatac tgcgcttctt cttcggcagg ttaaagcctg tatcggattg
     1261 gcaggaaaaa ctgctgaaaa agttccggga ggatttcgga gacagcctat aatacttaag
     1321 gcggctttta ctaaagccgc ctcgtttttt tataggccca tgctcatgtt cctggccaat
     1381 tcaaattcct tcagaacttc ctcggaaggt tcctcgctca gcagggaaac caccactata
     1441 gctacacatg acaggacaaa agccgggaga agttcgtaga tatcaaatac accccctaag
     1501 ggcctgacta acaatttcca gacaaagacc gtaattccac cggtgagcat cccggccatg
     1561 gcccctgccc tgctgaccct cttccaaaac agggaaaaca gcaccgtcgg tccgaaggtg
     1621 gcaccaaagc ccgcccaggc aaaggaaacc accttaaaga tgacgctatt ttcatccagg
     1681 gctataaata tggcaaagag cgagatgacc aaaagggcaa ttctggtcat ggtcaacact
     1741 tcccggtcac tggcttccct tttgattaga ccatggtaga tgttcttgga aaaggccgat
     1801 gcggaaataa tcaagtatga gtccgccgaa ctgatggtag ctgcaaggat tccggacatc
     1861 acgataccgg cgaatatggg gacgaaaaag ctggtagaca tcaggatgaa gatattctcc
     1921 gcggcgcttt gggtaagata ggcatctgga aatattaccc tgccgatgat tcctatgcca
     1981 atagatgctc cgagggagat ggcgcaccaa agggtgccta ttctcctgga caaggtcagc
     2041 tccgaggttt cacgaatggc cataaatttt aggagcacgt ggggcatgcc aaaatagcct
     2101 aagccccagg acaaggtgga tatcaccgta agccagccgt aactgccggc ctcgccaaaa
     2161 acgggctttc catccaccac ctgctgcact ccatctgcca gttggggaga agctattccg
     2221 aaaaactcga aaaatccggg tatctgcttt gcattttgta cgacctgcga taggccgcct
     2281 gcagcaccga ttcccgcaac caataccgcc accagggcaa agatcatgac gactccctgc
     2341 ataaaatctg aggcactttc agccaaaaaa cctcccacaa aggtatagat gatgacgaag
     2401 agggcccctg ccaccatcat gtaaacatac ttaaaattaa aaagagtgct gaagagttta
     2461 cccactgtaa cgaagcagct agctgcatat accgtaaaga aaaccaagat aaaaagagcg
     2521 gccagtgtca tcagtacctt gttctcttcc ttaaaccttt tgctgatgaa gtctgggata
     2581 gtgattgagt tgccggcaat ttccgaatag gcccgtattc tctttgccac aaagagccag
     2641 tttaaatatg tacctacgag aagaccgatg cccgtccaaa aggcgtcact caaacccacc
     2701 cagtaggcga cgccgggcaa gcccatcaag agccagccgc tcatgtcgga cgcctctgcg
     2761 gctaaggcgg taacccatgg ccccagggac cttccaccta tgaggaaatt ttccgtgctc
     2821 tcactggccg tcttggcata gttaaggcca atggcaatga ctgctccaat gtaaagtacc
     2881 attgcgatta agatctgaat actcatggta tgtcacctcc tgttatataa catttcagaa
     2941 aagtatacca cataaatcaa ttcatctcaa gcataaaatt cttgaaagca gcctgggatg
     3001 tgtcagttta taataataaa aggcggctat ctaagccgcc tggtcattgc tcttaagcat
     3061 atgcaaaccg aatcccttta tatcatgccc tctctattca gctaatgctt ctgcttcttc
     3121 gatttcctcc atcatctcca ttatttgctg catatattct tcctggctga ttgcatcact
     3181 cagatcggga agttcgagtt caactccgta atcgtacatc tgatagtcgc ctttcatatt
     3241 aatttggaaa accatggggc cttcaggaat cgctgctttt tcttcttcat ccatttctgt
     3301 cacttccgca attgcttcta tcaattgttt tacatcaatt accatattca aatcaatctt
     3361 catgtaatcg gatagcagag tttctttatt aataaaggta tctatatagt aatcaaaaac
     3421 tatgttattc agaacaatat caaatatttg ctctaactgt gcactgacat cttcgccatc
     3481 cccagcttgt gcggcaaaag ctgccataaa agaacccaaa ttgatatttc ctaggacttg
     3541 ttccaccaaa ctcctataac tttcagcatc aatgtaattc ttgatagtgt aatactccct
     3601 accctcaatt actgcatcat cgccaaacac attgataact cctgcttcat tcatcatctc
     3661 tatcgattgt tggggagtca tttgaattaa gctatcaaaa ccaatcattt cttccattcc
     3721 ggttaaatcc tggtatatcc actggccagt gtcggggttc ttagtataaa tgacattatc
     3781 gtgccaaacc gtctccgtaa ctattccttc agcaagacct gatgcttcca tttcttcggg
     3841 gctgacccct agcatttcaa gaggcatctt catggtctgc ttcatatgca tgctcatcgg
     3901 ctcatttcgg aaaactcctt caataacgag ctccatatta atgggcactc ccattaatgc
     3961 tttcagaact tcatcgccct gcaaatcaag cccaatttca tttacagctt tcatcctgta
     4021 ggtatttgct tcttttagaa gttccgaact ctttatgacc agctcatctg ccgttaaatc
     4081 tccggatttt tccctccagg atacgattac atgccaatta ttgttatccc atgaaacttt
     4141 tccgccctgg ctctcgaaaa attcgcgaat gggcacaatc ggatcatctc ccagttctag
     4201 acctgggatt ctttttag
//
LOCUS       DAONUO010000178         4210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14910_length_4210_cov_3.977617, whole genome shotgun sequence.
ACCESSION   DAONUO010000178 DAONUO010000000
VERSION     DAONUO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14910_length_4210_cov_3.977617"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..154
                     /gene="rpmB"
                     /locus_tag="PK768_06990"
     CDS             8..154
                     /gene="rpmB"
                     /locus_tag="PK768_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQA60752.1"
                     /translation="MKISHSHIRTKRTWAPNIRRVKADVDGHIKRIKVCTGCLRSGKV
                     KRAQ"
     gene            complement(280..438)
                     /locus_tag="PK768_06995"
     CDS             complement(280..438)
                     /locus_tag="PK768_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60753.1"
                     /translation="MWKRLNIKQILGIAATIAGIAVLIVGLPSWMWFLAAGASLIWCG
                     WMLFTTKW"
     gene            complement(517..1167)
                     /gene="rpe"
                     /locus_tag="PK768_07000"
     CDS             complement(517..1167)
                     /gene="rpe"
                     /locus_tag="PK768_07000"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQA60754.1"
                     /translation="MVKVAPSILSADFSRLAEEVKRVEQGGADLLHIDVMDGHFVPNI
                     TFGPAVIASIKGKTKLPFDVHLMIEEPEKYLNDFIRAGADILTVHAESTVHLHRLVSH
                     IKSQGIKPGVALNPSTPLDAIKYVLDDIDLVLIMSVNPGFGGQKFIPRILDKISELRS
                     IVEQNQYDILIEVDGGINEDNASEIIDAGADILVAGSAIYESPNPEDVIKRLKNNN"
     gene            complement(1195..3060)
                     /gene="pknB"
                     /locus_tag="PK768_07005"
     CDS             complement(1195..3060)
                     /gene="pknB"
                     /locus_tag="PK768_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HQA60755.1"
                     /translation="MIGKILGNRYQILEKVSGGGMAFVYKARCNLLNRIVAIKILKPE
                     FAEDEDFVRRFRMEAQAAASLSHPNIVGIYDVGQEDGLYYIVMEYVEGNTLKDLIRQE
                     APLPVTEVIEVGIQICEALDCAHKNMIVHRDIKSQNIMITADGRVKVTDFGIARATGG
                     TTITNTGNVFGSVQYFSPEQARSDKVDERSDLYSVGIVLYEAFTGRLPFNGETPVSIA
                     LKQIQEKPEPISNILPGFPQQLETIVQKCLAKSPEDRYQSAEELKKDLRKALSSPEVI
                     SYKPENLEHTLVLDSITQDKSDKNVANSISKVRRPRNILRGIGIAALFVILFGVFSYL
                     GAIVARKYFEVPETVVPNVVGMQEEEAIQLLKERNLDYNILDRVFDVAPAGQVIDQDP
                     KGSQRVKTNRPPVDLIISKGPRASTVPNIIGITEQEGIALLQNSGFEPGRIIRQYSDE
                     VPEGIIMDQNPRADLTLSEGEMINFTVSLGPELLDVPNLVGKTLEEAENELKKLNIRY
                     NVTERADEAPKDTVIEQSPKPYEAMEPGTVVNLVISSGPAQPKRIVIEPILLPSEPSE
                     ITVKVVVSDDLGDKRTVYFEKHSPEDSPLSIPVEGVGNMEVEVWLNDMLYFKGNG"
     gene            complement(3057..3812)
                     /locus_tag="PK768_07010"
     CDS             complement(3057..3812)
                     /locus_tag="PK768_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HQA60756.1"
                     /translation="MIGVIKTDVGKVRANNEDAYYFPLKRGNSPELYIVADGMGGHFG
                     GEIASEIAVNEVSSYINSNLSPDSDDQKVKGIIESAIISANNKILSYASENNVLSGMG
                     TTITMALFHNGSLYIGHVGDSRAYRIRDNNIEQLTRDHSLVWQMAEEGRITMEEAESH
                     PLKNIITKALGADERIEPDIMEFDLKDNDIIILCSDGLTTMLDNDIIKSIASSFEPEE
                     AAVKLIDKANSKGGLDNITVGIIKVGEVGMRET"
     gene            complement(3817..>4210)
                     /locus_tag="PK768_07015"
     CDS             complement(3817..>4210)
                     /locus_tag="PK768_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQA60757.1"
                     /translation="SLHAPNDEIRSSLMPINRRYPILQLLDACKYYIMKTNRRITFEY
                     ALISGINDDEKCAHELSNLLRDMLCHVNLIPLNPVKGKTFKRSKPSQVRRFEKILSAN
                     GISATVRRELGSDIQAACGQLRRSLLMR"
BASE COUNT         1137 a          997 c          752 g         1324 t
ORIGIN      
        1 gagaggcatg aaaattagcc attcccacat aagaaccaag cgcacatggg ctccaaatat
       61 tcgcagggtt aaagctgatg tggatggaca tataaagcgc atcaaggtat gcaccggctg
      121 cctacgttcg gggaaagtta agcgtgcgca ataaaaatta gcgtgccaag tagcacgctt
      181 ttttaattat tttgaccaaa gagccccagt attgctttaa cgaatttacc aagggttttc
      241 gggagtttca atacatatat ccgcattaac ccaccccttt caccatttag tggtaaacag
      301 catccaccca caccagatca aggaagctcc tgcagctaag aaccacatcc acgatggcag
      361 tcccacaatt aataccgcta tgccagcaat agtagcggct atgcccagta tctgcttgat
      421 attaaggcgc ttccacactt tgccaccccc ccgctggcaa aaatcccggt tattttatta
      481 tattcagcat aaagtgtttc tgataccaaa caatgattaa ttattgtttt ttaatctctt
      541 tattacatct tcgggatttg gagattcgta aatggcagac cctgctacta gtatatctgc
      601 tcctgcatct attatttcag atgcattatc ctcatttatt cctccatcca cttcaatcaa
      661 aatatcgtat tgattttgct ctacaatgga cctcaattcc gatatcttgt caaggattcg
      721 ggggatgaac ttttgaccgc caaagcctgg atttacactc ataatgagaa ccaggtctat
      781 atcatcaagg acgtatttta tggcatctaa aggagtagag gggtttaggg caactcccgg
      841 cttaattccc tgacttttaa tatggcttac caatcgatgc agatgtacgg ttgattccgc
      901 atggacggta agtatatcag cacctgccct tataaaatca tttaagtatt tttcaggttc
      961 ttcaatcata aggtgaacat caaagggaag cttagtcttc cctttgatag acgctattac
     1021 cgcaggacca aaggtaatgt tcggcacaaa atgaccgtcc atcacatcta tgtgcaaaag
     1081 gtcagcaccg ccttgttcca ctcgcttcac ttcttcagct aaacgactaa aatcagcaga
     1141 taaaatcgaa ggtgcaactt taaccaccaa tataacctct ttctttaaat agatttatcc
     1201 gttaccttta aaatagagca tatcatttaa ccatacttct acttccatat tgcccacacc
     1261 ttccacggga atgcttaagg ggctgtcttc aggagaatgc ttctcaaagt acacggtacg
     1321 cttatcacct aaatcatctg agacaaccac ttttaccgtt atctccgaag gctctgatgg
     1381 cagaagtatg ggctctatca cgattctctt cggttgagca gggccgctac tgattacaag
     1441 gttaactaca gttccgggtt ccatagcttc atatggctta ggactttgct ctattaccgt
     1501 atccttggga gcctcatcgg ctctctcggt aacattatat cttatattta atttttttaa
     1561 ctcattttca gcttcctcta aggtctttcc gactaaattg ggcacatcta gcagctccgg
     1621 ccctaaacta acggtaaagt taatcatttc gccctcagaa agagtaagat ctgcccgagg
     1681 attttgatcc attatgattc cttccggcac ttcatcagag tactggcgaa taatacgccc
     1741 cggctcaaag ccgctatttt gtaataaggc aattccttcc tgctcggtta taccaattat
     1801 gttgggaact gtactggctc ttggcccttt gctaattatt aaatcaaccg gaggcctgtt
     1861 ggtttttacc ctttgacttc cttttgggtc ctggtctatt acctggcctg ccggagcaac
     1921 atcaaaaact ctgtccaata tattatagtc aagatttctc tcttttaata attgtatagc
     1981 ctcttcctct tgcatgccaa cgacatttgg tacaaccgtt tcgggtacct caaaatattt
     2041 tctcgccact attgctccaa gataagaaaa cacaccaaag aggatcacaa agagagcagc
     2101 gatgcctata ccccttaaaa tgttcctcgg ccttctcacc ttagaaatag aattggcaac
     2161 atttttatca gatttatctt gtgtaatact atcaagtacc aatgtgtgct ccagattttc
     2221 aggtttataa ctgattactt caggacttga caaagccttc cttaaatcct ttttcaattc
     2281 ctcggcactt tgatatctat cttcagggga ttttgccaaa catttttgaa ctattgtttc
     2341 cagctgctga ggaaagccag gaagtatatt tgaaattggt tcgggctttt cctgaatctg
     2401 ctttagtgct atggatacag gagtctcacc attaaagggc aatcttcccg taaacgcttc
     2461 atacagcacg atgccaacag agtacagatc tgaacgctca tccaccttgt cacttctagc
     2521 ttgctccggc gagaaatatt gaaccgaacc aaaaacattg cctgtattag taatagtggt
     2581 tcctcccgta gcacgagcga ttccgaaatc agtcacttta actctgccgt ctgctgttat
     2641 catgatattt tgggatttaa tatccctgtg aactatcata tttttgtgag cacagtccaa
     2701 ggcttcgcaa atttgaatgc caacttcgat aacttctgta acgggaaggg gagcttcttg
     2761 ccttatcaaa tcttttaagg tattgccttc aacgtattcc atcactatat aatacagccc
     2821 gtcctcctgc cccacatcat aaatcccaac aatatttgga tgtgacagac tagctgccgc
     2881 ttgggcctcc attctaaagc gcctaacgaa atcttcatcc tcagcaaatt ccggttttaa
     2941 aatctttatc gcaacaattc tattgagcag attgcatcgt gctttataaa cgaaagccat
     3001 gcctccgccg ctgactttct ccaaaatttg gtatctgttt cccaagattt tgcctatcat
     3061 gtttctctca tccccacttc gcccacttta attattccaa ccgtaatatt gtctagaccg
     3121 ccctttgaat ttgctttatc aataagtttt acagctgcct cttcaggttc aaaactagag
     3181 gcaatgctct ttataatgtc attgtccagc atagtagtta aaccgtcgct acataaaatg
     3241 attatatcat tatcttttaa atcaaactcc attatatccg gttctattct ttcatcggcg
     3301 cccagggctt ttgtgatgat atttttcaac gggtgacttt cagcctcttc catggtaatg
     3361 cggccctctt cagccatttg ccatacgaga gagtgatccc tggtcaactg ttcaatgtta
     3421 ttatctctta ttctgtatgc cctgctgtcg cctacatgcc ctatgtacaa gctgccgtta
     3481 tgaaacaggg ccatagtaat agtggtcccc atgcctgaaa gcacattgtt ctctgatgca
     3541 taagaaagaa ttttattgtt ggcgctaatt atagcagatt ctataatacc ttttactttt
     3601 tgatcatcgg aatcaggaga tagattgctg ttaatgtatg atgagacctc atttacggca
     3661 atttcactgg ctatttctcc tccgaagtga cctcccatac cgtcagcaac aatatagagt
     3721 tcgggagagt ttcccctttt caggggaaaa taatatgcat cttcattgtt ggctctaact
     3781 ttccccacat cggttttaat tactcctatc atggtttcac ctcatcaata gacttcgcct
     3841 cagttgacca caggcagctt gtatatcact gcctaattcc ctacgaaccg ttgcagatat
     3901 accgttggct gatagaattt tttcaaacct gcgaacctgg gagggcttgc ttctcttaaa
     3961 agttttcccc ttaaccggat ttagtggtat tagatttaca tggcatagca tatcccttag
     4021 taagtttgag agttcatgcg cgcatttttc atcatcattg attccggata tcaatgcgta
     4081 ttcaaaagtt atgcgcctat ttgtcttcat tatataatat ttgcaggcat ctaacaattg
     4141 caaaattgga tatctccgat taataggcat caaggaactt ctgatttcat cattgggagc
     4201 gtgaagggaa
//
LOCUS       DAONUO010000179         4199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_14947_length_4199_cov_3.575531, whole genome shotgun sequence.
ACCESSION   DAONUO010000179 DAONUO010000000
VERSION     DAONUO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14947_length_4199_cov_3.575531"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PK768_07020"
     CDS             <1..1244
                     /locus_tag="PK768_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60758.1"
                     /translation="YISIPGDLKLRSANLYKDYDNPDQKVWSLEWDKSGDNNEGGYYI
                     YARVNAITSELLSFDIYDYGKASQEFKQKYDREAAQKKAEEFLKKHQPKRFENVELEE
                     FYGKVDFPEKVQRHNFSYTRLVKNIPYTANGFYVTVDAETGKITNYRMNWHEKDFPSA
                     EGVLEKNDAEARFLRDIGLELVYTALYNPEENSSDYKLVYKLKPASSYTFDAFDFKPL
                     DYSGKAIEEEIRTNFTDIKGHWAEDDIQLLVDLGIIKSEEKLFRPDDNMTEGEFVKLL
                     LIAKGYRISDDIPLLPIIRLESGDTKIDGEIQKYIDEAIKLGWVKENEASVHGVLSRE
                     KAAAFLIRAMGFEQVASIPEIFKDLADDSASIKAEYKGHAAIAMGLKLMTATGGNFNP
                     KGNVSRAEGATILVRMLRSSS"
     gene            1885..3783
                     /locus_tag="PK768_07025"
     CDS             1885..3783
                     /locus_tag="PK768_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA60759.1"
                     /translation="MAILNVSGLSKSFGIDTILENVNFLIEEKDKIGLIGINGSGKST
                     LLKILAGKIPYDSGNIYMEKNLKIGYLSQDAKFESHKTINQALSSIFKELQIQESNLR
                     KLEKLMSSPDVYQDEKQLENLMHQYSALFEEFKSMGGFEVESRIRGVIRGLGFDDENV
                     PISTLSGGQKTRLALGRVLLQSPDLLLLDEPTNYLDLESIQWLEAFLKDYNKAVLIVS
                     HDRYFLDKVVNKIFDLENGSITVYDGNYSYFINKKHAMMKVLKKHEENRQKEIERLQK
                     NIETFISHRKYSQAESRRKRLEELLPKSVIKHSRADINMRFNVHESSGKEVLHIEDLS
                     FSYGRKNILDSLNIKVYRGERIGIIGPNGIGKSTLLKLLAGELEPQEGSIRFGHNVCP
                     VYFDQEQQDLSPDATILEEVYNSAPGLTLTEIRTFLGRLLFTGDEVEKQIKVLSGGEK
                     SRVALAKAILQGANLLLLDEPTNHLDILSKENLEEALQSFKGTIIAVSHDRYFLSKIA
                     TRIWEFDKGGIADYNGNFEYYLEKKSELNKEITPEKRENKTQLKKAKLQERKERERKR
                     QFQKRLKQLEENILKKEEELEKLEYMLCEPEVYSNPEKAQEVNIRYNQILTDLEDLYE
                     MLDEQEEL"
     gene            3786..>4199
                     /locus_tag="PK768_07030"
     CDS             3786..>4199
                     /locus_tag="PK768_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQA60760.1"
                     /translation="MAFKENFITLGIETSCDETSVSVVKDGRKILSNTIFSQIKQHAK
                     YGGVVPEIASRQHLEAISHVTQMALEEASIGFDDVDLVAVTQGPGLIGALLVGVSYAK
                     GLAYALNKPLIGVNHIEGHIFANFLENDFTPPFLCL"
BASE COUNT         1323 a          789 c          995 g         1092 t
ORIGIN      
        1 aatatatatc gattccgggt gatttaaagc tgcgctcggc gaacctctac aaggattacg
       61 ataatcccga ccaaaaggta tggagcttag agtgggataa aagcggtgac aacaatgaag
      121 gtggctatta tatatacgcc cgggtaaatg ccattacttc cgaacttcta agctttgaca
      181 tttacgacta cggcaaagct tcacaggagt ttaagcaaaa atacgacagg gaggccgcac
      241 agaaaaaggc tgaggagttt ttgaagaagc accagccaaa gcgctttgaa aatgttgagc
      301 ttgaagagtt ctatggaaag gtagattttc ctgaaaaggt tcaaagacat aatttcagct
      361 ataccagatt ggtaaaaaat attccttaca ctgccaatgg attttatgta accgttgatg
      421 ctgaaacggg taaaatcacc aattatcgca tgaattggca tgaaaaagac tttccaagtg
      481 ccgaaggcgt tttggaaaag aacgatgctg aagctcgttt tctaagagac atcggtttgg
      541 aattagtgta tacagcttta tataatcctg aagaaaacag cagtgattac aagctggttt
      601 acaagctcaa acctgcgtca tcttacacct ttgatgcctt tgattttaag cccttagact
      661 atagcggcaa ggccatcgaa gaagaaataa ggaccaactt taccgatata aaaggtcatt
      721 gggctgaaga tgacatacag cttctcgtgg atttaggaat cataaaatct gaagaaaagc
      781 tatttaggcc cgatgataat atgactgaag gcgagtttgt caagctcttg ttgattgcaa
      841 aaggctatcg aatttcagat gatatcccgc ttttgcccat cataaggctt gaatcgggag
      901 acactaaaat cgacggagaa attcagaagt atatcgatga agcgataaaa ctgggttggg
      961 ttaaagaaaa tgaagcttcg gtccatggag tcctttcccg agaaaaagca gcagccttct
     1021 taataagggc aatgggattt gaacaagttg caagcattcc cgagatcttc aaagacctag
     1081 cagatgattc tgcctccata aaagccgaat ataaaggcca cgcagccata gcaatgggtc
     1141 ttaaacttat gacagcaaca ggcggcaatt tcaacccgaa gggcaatgtg agtcgcgcag
     1201 aaggggcaac tattctggtg cgaatgctca ggtcaagttc ttaagttaaa actgctcacc
     1261 tggaacttgt gaataattga agcgataggg gcaaaataat atcgtagggt tcgcagtggc
     1321 tgatccaaga ctgccggagg atcaggtgtg tggttctctg gtagtcggcc aaaggtagat
     1381 gtaaggtcct cgggctttac atctgccggc gggcagaggg ctatgcgtct tataaggctc
     1441 tgtggtagcc caactgctat catagggtcg aaatagatgt atagtcttcg agctaagatc
     1501 accatgggtg ccgcagatta atccaatata gagtattgga catggtactt tgttgcatta
     1561 attggaggtc tgtagcagcc gggaggttgc tgccggcttc taaggtactc atgctggtcg
     1621 agcataggtt gttatgggct ttgtaatggt ctattgcagc cgaaagattg cagtgggcta
     1681 tgtagaggtt catagcagct gtagcgaagg tcactgcgag ctcttttgtt ttgtgtcatt
     1741 atccctgcag gaaatttacc tgcagggttt tattttgcct ttctttttgc taatataata
     1801 caagttttat tgtctttgcc cccgggctta tgttaacata tatttgactt gagattatgt
     1861 ctatagaatt catggtgata taggatggca atattaaacg tatccggctt gagtaaaagt
     1921 tttggcattg acaccattct tgaaaatgtc aattttttaa tcgaagaaaa ggataaaata
     1981 ggtttgatag gtattaatgg ttcagggaaa tccactttgc tcaagatact ggcaggtaag
     2041 ataccttatg acagcggaaa tatttatatg gaaaagaatt taaaaatcgg gtatttatct
     2101 caagatgcaa agtttgaaag ccataaaact attaatcagg ccctttcatc aatttttaaa
     2161 gagcttcaaa tccaggagag caatcttaga aagcttgaaa aattgatgtc ctctccggat
     2221 gtttatcaag atgaaaagca actagaaaac ttaatgcacc agtattctgc tctctttgaa
     2281 gaatttaagt caatgggcgg ttttgaggta gaaagtcgca tacgtggtgt tatccgcggc
     2341 ctaggctttg atgatgagaa tgttcctatc tcgacattaa gcggtggtca aaaaacccgt
     2401 cttgccttgg gtagggtact cctacagtca cccgacctat tgcttttaga cgagcctacc
     2461 aactatctgg atttagaatc gattcaatgg ctggaagctt ttttgaagga ttacaacaag
     2521 gctgtcttga ttgtatctca tgaccgttat ttcctggata aggtggtaaa taaaattttt
     2581 gatttggaaa acggaagtat tacagtttac gacggcaatt attcctactt tataaataaa
     2641 aaacatgcta tgatgaaggt gttaaaaaaa cacgaagaaa acaggcaaaa ggaaatagaa
     2701 aggcttcaaa agaacattga gaccttcatc tctcacagaa aatactcgca ggcagaaagc
     2761 cgtcgaaaaa ggcttgaaga gcttcttccc aaatccgtga taaagcatag cagggccgac
     2821 atcaatatga gattcaatgt gcacgaaagc agcggcaaag aagtgcttca tattgaggat
     2881 ttgagctttt cctatggaag aaagaacatc cttgactcgt taaatataaa agtgtaccga
     2941 ggtgaaagga taggcataat cggcccaaac ggcataggca aatcaacgct tttaaagctc
     3001 ttggcaggag agctggaacc ccaggaaggc agcatacgtt ttggccacaa tgtttgcccg
     3061 gtgtattttg accaggaaca gcaagacctt tcccccgatg ccacaatcct tgaagaggta
     3121 tataattccg caccggggct cactttgacg gagatcagga cttttctggg aagacttctc
     3181 ttcaccggtg atgaggtaga aaagcaaata aaggtcttga gcggcggcga aaaaagcaga
     3241 gttgccttgg ccaaagccat tttgcagggt gcaaatctcc tccttttaga cgaacctacg
     3301 aaccatttgg atatattgtc caaggaaaac ttggaagagg cattacagtc ttttaaggga
     3361 accattatcg ccgtctccca cgaccgatat ttcttgtcaa aaatcgctac ccggatttgg
     3421 gagttcgata agggcggcat agcggattac aatggaaatt tcgagtacta ccttgagaaa
     3481 aaaagtgaac tgaataagga aataacaccg gaaaaaaggg aaaataagac gcagcttaag
     3541 aaagccaagc tccaggaaag aaaagaacgg gaaaggaagc ggcagttcca aaagcgattg
     3601 aagcagctgg aggagaatat tttaaagaaa gaagaagagc tggaaaagct ggaatacatg
     3661 ctgtgcgagc ccgaggttta cagtaacccc gaaaaggcac aggaagtaaa catcaggtac
     3721 aatcagatcc ttaccgactt agaggatctc tatgaaatgt tagatgagca ggaggaattg
     3781 taataatggc cttcaaagaa aactttatca ctctcggcat agaaacctca tgtgatgaga
     3841 catcagtatc agtagtaaag gacggcagaa agatactgtc aaatactatt ttttctcaga
     3901 taaaacagca cgcaaagtac ggaggtgtgg tgcccgaaat cgcctcgcgc cagcatcttg
     3961 aagcaatatc ccatgtgacg caaatggccc ttgaagaagc ttcaattggc tttgatgacg
     4021 ttgatttggt ggcagtaacc cagggcccgg gcttgatagg agccttgctg gtaggagtat
     4081 cctacgccaa aggcctggca tatgctttaa acaagccctt gataggtgtc aaccacattg
     4141 aagggcacat ttttgctaat ttcctggaaa atgactttac cccgccattt ctgtgtctg
//
LOCUS       DAONUO010000180         4178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15031_length_4178_cov_4.010187, whole genome shotgun sequence.
ACCESSION   DAONUO010000180 DAONUO010000000
VERSION     DAONUO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15031_length_4178_cov_4.010187"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK768_07035"
     CDS             complement(<1..207)
                     /locus_tag="PK768_07035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQA60761.1"
                     /translation="MFNFGYYIIGNNIEALEVAGLYFAISFFILVLDLFTKHIVQKNM
                     VPHQSIPVIKNVFHITYVQNTGAAF"
     gene            complement(246..872)
                     /locus_tag="PK768_07040"
     CDS             complement(246..872)
                     /locus_tag="PK768_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HQA60762.1"
                     /translation="MNIEHYKRQLESLKSRLEKQMELLSKRGSEPLKESIGELSGYDN
                     HPADLGAETFERSKDLGLKGNSKVLLMKVNHAIDRINNGSYGVCENCGKNIERERLEA
                     LPYTTFCIDCKKSEEKPGDHSSRPLEEEVLKFPFGRSSRNGKDTVGYDGEDAWQDVAR
                     YGTSDGPQDIPGAVDYSETYVDGEEPRGIVEGTDTIINERDLDEDDRN"
     gene            complement(904..1191)
                     /locus_tag="PK768_07045"
     CDS             complement(904..1191)
                     /locus_tag="PK768_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HQA60763.1"
                     /translation="MTDHKTYEKLSQKIDELSLNIERLNLAEYVELLRNPKRLLYMNL
                     LGGIARGFGTAIGFTLLGALAIYFLQRIVILRLPIIGTFIADIIKIVQKQL"
     gene            complement(1257..1814)
                     /locus_tag="PK768_07050"
     CDS             complement(1257..1814)
                     /locus_tag="PK768_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HQA60764.1"
                     /translation="MDISPLEIQKKEFSRSIRGYNTEEVDEFLDNLLANYERIYKENR
                     DLREQIQLLHEKMQSYKDIETTMKNALVLAEKTAEEVKSNAEREKEAILKEARLHAKE
                     IIQQAKEQFNQINNQNEELRQQFCLYKIRFKNFLESQLEYLDSLELDLFADSASSCES
                     ALKAAAASDGEENRQDTDREVHNQQ"
     gene            complement(1828..2103)
                     /locus_tag="PK768_07055"
     CDS             complement(1828..2103)
                     /locus_tag="PK768_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQA60765.1"
                     /translation="MIRIVNVFFQFIDLMILLRVILSFFPNPINPTISSFIYDFTEPI
                     LAPFRELLERFIPRGSWPYLDFSPLIALLFLDIVRVIIVRLLYGIFW"
     gene            complement(2108..2548)
                     /gene="sepF"
                     /locus_tag="PK768_07060"
     CDS             complement(2108..2548)
                     /gene="sepF"
                     /locus_tag="PK768_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HQA60766.1"
                     /translation="MSNKTFFKKILYFLGIEDEMPEEAEKQDFEPYIARPEPKSRGRV
                     INIHQTNKNKMIIFKPGSFEEVSEITDEVKNRRAVIVNFEKLDKETAKRILDFMSGSI
                     YALNGTVKKIGPGIFIFAPDNVDVSGVEGDEENQDTVSNIKLRG"
     gene            complement(2571..3260)
                     /locus_tag="PK768_07065"
     CDS             complement(2571..3260)
                     /locus_tag="PK768_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQA60767.1"
                     /translation="MSYLKQNIQQIKKRIEAAAERSGRNPEDIYLIAVTKNFSAELIQ
                     EAVDNGIVFLGENRVQEAKSKVDLVKGNIEWHLIGHLQRNKVKTAIDIFTMIQSLDSW
                     RLAEEIQKRAQQADKVIDVLVQVNIGSEETKYGIDPDDTEVFIEKVATLPNLKVRGLM
                     AIAPFKENPEDVRAYFRHLREIFQSIKEKSIENVSMEYLSMGMSNDFEVAIEEGANMV
                     RIGTGIFGTRK"
     gene            complement(3214..>4178)
                     /locus_tag="PK768_07070"
     CDS             complement(3214..>4178)
                     /locus_tag="PK768_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQA60768.1"
                     /translation="VRVGTPLFIVTTDEKQKQRFQNEISELEEKIESLEGNPGSSSMS
                     LSLIEKSIDSTTQKIKEATENGEFDKVRSLQDELTRLAEERQKLLESNEASINTLKEQ
                     LNKMKEEVSKIDIVIYASEAGIVSLYIDGLEDVLVPDRAKDISHAQLTAVVEGLTKTS
                     LPKEVNMNQAVLKIIDNFSWYIAFKAEKGLKEGRDYYIKIDNDREIRGRLASINDNGI
                     GFFSINTDLDSLLDSRKIDLEVIVGTHKGCMIPKEGIFYDNGVEGVYVFERGEKCFKP
                     VELVVEDEDNIIVNGIKQGDKILLNKRGLDELFKAKYTTNQKKN"
BASE COUNT         1099 a          961 c          622 g         1496 t
ORIGIN      
        1 aaaggcagct cctgtgtttt gaacatatgt gatatgaaaa acatttttaa taactgggat
       61 agactgatgt ggcaccatgt ttttttgtac tatgtgtttt gtgaacaaat ctagaactaa
      121 tatgaaaaaa ctaattgcga aatacaaacc ggctacctcc aaggcttcaa tgttatttcc
      181 aattatataa tacccgaagt taaacatcaa tgaaaaaatg ccctacaagg gcatttcttt
      241 tccggttaat ttctatcatc ttcatcgaga tctctttcat ttatgatcgt gtcagttcct
      301 tcaactatac cgcgcggttc ttcgccatcc acataagttt cgctataatc tacagccccc
      361 ggtatgtctt ggggcccgtc agacgtaccg tatctcgcta catcctgcca ggcatcttca
      421 ccatcatatc ctactgtgtc ttttccattc cgagagcttc ttccaaaggg aaattttaag
      481 acttcctctt cgagtggcct actactgtga tcacctggct tctcctcgga tttcttgcaa
      541 tctatgcaaa aagtcgtgta aggtagggct tccaatctct ccctttcaat attcttgcca
      601 cagttttcac atactccgta agacccattg tttattctat caatagcatg gttcaccttc
      661 ataagcaaaa cctttgagtt gccctttaga cccaaatcct ttgatctctc aaaggtttcg
      721 gcacccaaat ctgccggatg gttatcgtag cccgatagct caccaatcga ttcctttagg
      781 ggttcagagc ctcgctttga cagtaattcc atttgctttt ccagcctgct ttttaagctt
      841 tcgagttgtc ttttgtaatg ttctatgttc aagtcgggcc tcctagcagt ttatattata
      901 cgctcaaagc tgtttttgaa caattttaat aatgtcggct ataaatgtac cgattattgg
      961 taatcttaaa atcacaattc gctggagaaa atatatggcc aaagccccta aaagggtaaa
     1021 gcctatagcc gttccgaagc ctctggcaat gcctcctaat aaattcatat acagcagtct
     1081 ctttggattt ctcagtaact caacatattc tgctaagttg agtcgttcaa tatttaagga
     1141 caattcatct attttttggc tcagcttttc ataagttttg tggtcagtca tgtggattcc
     1201 ctcaaaatta atttatccaa ccgaccgagt tttattccta attatcatag acgagtttat
     1261 tgctgattat gtacctctct atctgtgtcc tgcctattct cttcaccgtc actagctgcc
     1321 gccgccttta aagcgctttc gcaagatgag gcgctatcgg caaacaggtc aagctccaat
     1381 gaatccaagt attccagttg ggactcaaga aagtttttaa accgtatttt atataggcaa
     1441 aactgctgcc tcaattcctc attttgattg ttaatttgat tgaactgctc tttggcttgc
     1501 tgtattattt cttttgcatg caatctggct tcttttaaaa ttgcttcttt ttccctctca
     1561 gcattgcttt ttacctcttc agcagtcttt tcagccaaaa caagggcatt tttcattgtt
     1621 gtctcaatat ctttatagct ttgcattttc tcatgcaaca attgaatttg ttctcttaaa
     1681 tcccgatttt ccttgtaaat tctttcatag tttgccagca gattgtctaa aaactcatca
     1741 acttcttctg tattatagcc tcttatgctt ctgctaaatt cttttttctg aatttccaaa
     1801 ggcgaaatgt ccatattttt catctcctta ccagaaaatc ccatacagta atcttacaat
     1861 gataactctc acaatgtcta aaaagagcag tgcaattaat ggtgaaaaat ccaaataagg
     1921 ccatgacccc ctagggataa atctttccaa caattctcta aatggtgcga gaatcggttc
     1981 agtaaagtcg tatatgaagc tgcttatggt aggattgata ggattgggga agaaggataa
     2041 aatgaccctc aataaaatca ttaagtctat aaattgaaaa aatacattga caattcttat
     2101 caaagattta cccccttaat ttgatatttg atacggtgtc ttgattttct tcatcccctt
     2161 ctactccgga aacatctaca ttgtcaggag caaatataaa gattcctggc ccaattttct
     2221 ttacggtgcc gtttaatgca taaatgctgc ccgacataaa atctagaatg cgctttgcgg
     2281 tttctttgtc aagcttttca aaattgacaa ttaccgccct cctatttttc acttcatccg
     2341 taatctcact aacctcttca aaggaaccgg gcttaaagat aatcatcttg tttttatttg
     2401 tttgatgaat attgattacc ctgcccctgc tttttggctc aggtcgggca atatagggct
     2461 caaaatcttg tttttcagct tcttcaggca tctcatcttc aatgcctaaa aaatacaata
     2521 tttttttaaa aaatgtctta ttgctcatac tatcattagc tccttcttat ttattttcta
     2581 gttccaaata tgcctgtgcc aattcttacc atgtttgccc cctcttctat tgccacttca
     2641 aagtcattgc tcattcccat agacaggtat tccatactca cattttcaat agatttttcc
     2701 tttatacttt gaaaaatctc gcgcaaatgc ctaaaataag cccttacatc ttcgggattt
     2761 tctttgaaag gagctattgc cattaaaccc ctgactttta aatttggaag tgttgcaact
     2821 ttttcgataa aaacttcagt atcatcaggg tctataccgt atttcgtctc ttcactgcct
     2881 atatttactt gaaccaggac atcaattact ttatccgcct gctgggccct tttttgtatt
     2941 tcttcggcta atctccaact gtcaagagac tgaatcatgg taaatatatc tattgctgtt
     3001 ttaaccttat tccgctgcag gtgacctatt aaatgccact ctatattgcc ctttacaagg
     3061 tctactttgc tcttggcttc ctgaaccctg ttttcgccaa gaaaaacaat accattgtcc
     3121 acggcttcct gaattaattc ggccgaaaaa ttttttgtca ccgcaatcaa ataaatgtct
     3181 tccgggtttc tcccggagcg ttcagctgca gcctcaattc tttttttgat ttgttgtata
     3241 ttttgcttta aataactcat ctaatcccct cttatttagt aaaattttat caccttgttt
     3301 tattccattt actattatat tatcttcatc ttcaactacc aactcaacgg gcttgaagca
     3361 tttttctccc cgctcgaaga cataaacccc ctcaacacca ttgtcataaa atatgccttc
     3421 ctttggtatc atgcaacctt tatgtgtccc cacaatcact tccaaatcta tttttctcga
     3481 atccagcaga ctgtctagat ccgtattaat tgaaaaaaag ccaatgccat tatcatttat
     3541 gctagcaagt cgccctctta tctccctatc attatctatt tttatataat aatctcttcc
     3601 ttctttaaga cccttttcag ctttaaatgc tatgtaccat gaaaaattat ctattatttt
     3661 taggacagct tgattcatgt taacttcttt tggcaggcta gtcttagtta acccctcaac
     3721 gacagcagtc agctgagcat gtgatatatc tttcgcccta tcgggaacca gaacatcttc
     3781 aaggccgtct atgtaaagac ttactattcc tgcctccgat gcatagatca ctatatcaat
     3841 tttactgact tcttccttca ttttatttaa ttgctctttt aatgtattga tacttgcttc
     3901 atttgattca agtagttttt gtctctcctc tgccaatctc gtcaactcat cttgtaatga
     3961 cctcactttg tcaaattcac cgttctccgt ggcctctttt attttttgag tggtactatc
     4021 tatggacttt tcaatcaaac tcaaggacat ggatgagctt cccggattgc cttccaatga
     4081 ctctatcttt tcttctaact cgcttatctc attttgaaaa cgttgctttt gtttttcatc
     4141 ggtggtcact ataaaaagag gtgtgccaac tctgaccc
//
LOCUS       DAONUO010000181         4171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15066_length_4171_cov_3.468173, whole genome shotgun sequence.
ACCESSION   DAONUO010000181 DAONUO010000000
VERSION     DAONUO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15066_length_4171_cov_3.468173"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PK768_07075"
     CDS             <1..310
                     /locus_tag="PK768_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQA60769.1"
                     /translation="TDTRPEEIEQYWVNAYSEIDTIENKLSVIEKCGYTSVAHFVLNE
                     KCWLNYYQPLLENAEEFLKKYDYADEVKEFLELGKIEADMYNRFKDYYSYVFYIAKKK
                     "
     gene            471..944
                     /locus_tag="PK768_07080"
     CDS             471..944
                     /locus_tag="PK768_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQA60770.1"
                     /translation="MGIEKVRQYFSKWNMEDRIQEFDVSSATVELAAKALNCEPARIA
                     KTLAFKVDEKPVLIVTAGDVKIDNKKYKQKFGAKAKMLSPEEVRELIGYEVGGVCPFG
                     VSEDVVVYLDESLKRFETVFPACGSSNSAIELSILELEKYSGYIEWIDVCNYPAG"
     gene            complement(897..1547)
                     /locus_tag="PK768_07085"
     CDS             complement(897..1547)
                     /locus_tag="PK768_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HQA60771.1"
                     /translation="MSVKTPFHVIPHEITPGDTFYSLAKHYNTTVDAICLANPEIDPN
                     FLQVGQNIYIPLSNPTAIAHIRGGPLRPQIQGVVTFYKGQGGTWVCVEVKGLPPYKRA
                     ANGQNPIGPHGFHIHEFGSCEVGDPENPFQGAGGHWNPTNQPHGNHAGDLPVLFSNNG
                     YARMCFFTNAFTPDDVIGKAVIIHENPDDYRTQPAGNAGKRLACGIITNVNPFYVT"
     gene            1819..2298
                     /locus_tag="PK768_07090"
     CDS             1819..2298
                     /locus_tag="PK768_07090"
                     /inference="COORDINATES: protein motif:HMM:NF039481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HQA60772.1"
                     /translation="MKIPTNLMHKPVIVCENYENIDGRNAYKSDAKGLSLGLAQWNDR
                     GKVDISAKVWRHTGEKWSRQSEELPLHRVLDLAIFVCRSYLYFQEAYRYPKLYDPENP
                     RIDRVGLQGDAMTVEVCTDNPMIDEDIKLFQRALSEDGELIGERLRVISRLLKEAGY"
     gene            2316..2687
                     /locus_tag="PK768_07095"
     CDS             2316..2687
                     /locus_tag="PK768_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQA60773.1"
                     /translation="MASKIDKKRRKELLEQYKQIKTYIGIIQIKNNVNGKIFIDTCSN
                     LKNRWFTLKWNLDMGRHPNSKLQKDWNELGQDAFAYEVLEKKEADKVDDIRWELKKMK
                     KKWLEKLQPYGDKGYNRPPKE"
     gene            2803..3360
                     /locus_tag="PK768_07100"
     CDS             2803..3360
                     /locus_tag="PK768_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQA60774.1"
                     /translation="MQITEDNFIEQLKNKNEKALEHVIDNYGWILKSVIKKHLFYLPD
                     YHEECLNDCLMSIWENISYYDPKKSSFKNWIGGVAKYKAINYVRKYLRDLENENIENV
                     TVITEDNALSQVLSKEISLETKKMLECLSEEDREIFMKLYFEEKNMDEISCDTGLAKP
                     VLYNRLSRGRKKMREAFSLKGGSSQ"
     gene            3357..>4171
                     /locus_tag="PK768_07105"
     CDS             3357..>4171
                     /locus_tag="PK768_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HQA60775.1"
                     /translation="MKDCNNVYELLNYMEIDLDDYEKEMLSDKEKRSLKGLFKKKRRQ
                     RVLMQIGTIAAIFFLAIGFFSQTNLGKNVYAAVQLKVAEISYSIGVSMGIERDIEPYA
                     NVVDQIVADNGIEVKLSSVIIDKDELIFSIIVNTNKAVDMFRFDYDIFINGKKLTNYG
                     ATGRAGSITDSDSLFFHTQAVDVEGIDLKDDIDVRIVLKDMELITGTSQEKRKGKWQF
                     DFTANGSELTANSRTIPIDYSFNIDNQEYILQEFRYNPVNQKIYGKVKGAFGA"
BASE COUNT         1421 a          644 c          974 g         1132 t
ORIGIN      
        1 aaccgataca agaccagagg aaattgagca gtactgggtt aatgcatact cggagattga
       61 tacaatagaa aacaagttat cagttatcga aaaatgcggg tatacttctg ttgctcattt
      121 tgtactgaat gaaaaatgtt ggttaaatta ttatcagcca cttttggaaa atgcggagga
      181 attcctaaaa aaatatgatt atgccgatga ggttaaggaa ttccttgaac taggtaaaat
      241 tgaagccgac atgtataata ggtttaagga ttattacagc tatgtttttt atattgcaaa
      301 gaaaaagtag tttagcggat gccaagacat ctatagttca ggaaatcatg gaacgggcaa
      361 atgcaaagat gaaaatgtgg tgagactggt aaaatgccac ttacatggac aagttcagga
      421 ggttttgtta aagtattgtt acagataagg attacttggg aggaagtttt gtgggaatag
      481 aaaaagtgag acaatatttc agtaagtgga atatggaaga taggatacag gaatttgatg
      541 tatccagtgc aacggtagag ctggcagcaa aagccttaaa ctgtgagccc gcacggattg
      601 caaagacctt ggcttttaag gtggatgaaa agcccgtcct catcgtaact gcaggggacg
      661 taaagataga taacaaaaaa tataagcaaa aatttggcgc aaaggccaag atgctttctc
      721 cggaagaagt gcgggagttg ataggttatg aggtgggcgg agtctgtccc tttggagtaa
      781 gtgaagacgt agttgtgtac cttgacgaat cccttaagcg ctttgaaact gtgttcccgg
      841 cctgtggaag cagcaacagt gccatcgaat tgagcatcct tgaacttgaa aaatactcag
      901 gttacataga atggattgac gtttgtaatt atcccgcagg ctaatcgctt gcctgcattt
      961 cctgcaggct gtgttcgata atcgtccggg ttttcatgaa ttatgactgc tttgcctatt
     1021 acatcatcag gtgtaaaggc atttgtgaaa aagcacattc tggcatagcc gttatttgaa
     1081 aacagcacag gcaaatcgcc ggcatggttt ccgtggggct gatttgtagg gttccagtgg
     1141 ccccctgctc cttgaaaggg attttcagga tcgcccacct cacagctccc aaattcatgt
     1201 atatgaaatc cgtgaggccc tatgggattt tgaccattgg cagcccgctt atacggaggc
     1261 aagcccttta cctcaacaca tacccaggtg ccgccttggc ccttgtaaaa agtcacaaca
     1321 ccttggattt ggggcctaag gggtcctcct ctgatgtgtg caatggctgt gggattagat
     1381 aatggtatat aaatgttttg tccgacttga agaaagttag ggtcaatttc cgggttggct
     1441 aagcatatag catctactgt tgtattgtaa tgcttggcta aagaatagaa agtatccccc
     1501 ggggtaattt cgtggggaat tacatgaaag ggagttttta ctgacattat tctcacctca
     1561 ataaattaag ctgctgtttc aatgcacaca gtatcacgtg aagatagcgg tatctcagag
     1621 gtttccatgc ttgaagtcat tatattacct ttttaagata ttatataagg ccaaattgaa
     1681 aaatgttcat aataacccct aaaaggggtt atttatattg gattggttaa tctaacattg
     1741 acccgctgag aaatataggt tatactaaat tagtaaatta gtaattaagt aatttagtaa
     1801 aatgtatagg aggtaaaaat gaagataccg acaaatttga tgcataaacc ggttattgtc
     1861 tgtgaaaact atgaaaatat tgatgggcga aatgcttaca aatctgacgc caaagggctt
     1921 tcactgggcc ttgcccagtg gaatgacagg ggaaaagtgg atatttccgc caaagtgtgg
     1981 cgccacacgg gagaaaaatg gtcccgtcaa tctgaagaat tgccgcttca ccgggtatta
     2041 gatttggcta tttttgtctg cagaagttat ttatatttcc aggaggccta tcgttatcct
     2101 aaattgtatg atccggaaaa tccgagaatt gacagagttg ggcttcaggg tgatgcaatg
     2161 actgtggagg tctgcacaga taatcccatg atcgacgagg acataaaatt gttccaacga
     2221 gctttaagtg aagacggaga gctgattgga gaaagacttc gagtaatatc aaggctgctg
     2281 aaggaagccg gatactagaa atagaggaga taagtatggc gagtaagata gataagaaaa
     2341 ggcggaagga attgcttgag caatataagc agattaagac ttatatagga attatacaaa
     2401 taaaaaacaa cgtaaatgga aagatattta tagacacttg ttcaaactta aaaaatagat
     2461 ggtttacttt aaaatggaac ctggacatgg gaagacaccc aaactccaaa ttacaaaaag
     2521 actggaatga attgggtcag gatgcctttg cttatgaagt tttggagaaa aaggaagcgg
     2581 acaaagtgga tgacatccgc tgggaactta agaagatgaa gaaaaagtgg cttgagaaac
     2641 tacaacccta tggagacaag ggttacaata gaccgccaaa ggaatgaggc ggtttttttg
     2701 taattttatt ttgatagatt ttctgttgcc aatgcgaagt aataaataga aggataaata
     2761 aaccccatat agagaatata ccaacaagga aggtggtgcc cgttgcaaat aactgaagat
     2821 aattttatcg aacaactgaa aaacaaaaac gaaaaagccc ttgaacatgt catcgacaac
     2881 tatggatgga tactaaaatc ggtaataaaa aagcatctct tttatcttcc ggattatcat
     2941 gaagagtgtt tgaacgattg cctgatgagt atctgggaaa atattagtta ttatgatccc
     3001 aaaaagtcca gcttcaaaaa ttggattgga ggagttgcaa aatacaaggc catcaattat
     3061 gtaaggaaat atttaaggga tttagaaaat gaaaatatag aaaatgttac tgttattaca
     3121 gaagataatg cattaagcca agttttatca aaagaaataa gcctggaaac caaaaaaatg
     3181 ctggaatgct tatcagaaga agacagggaa attttcatga agctatactt cgaagaaaaa
     3241 aacatggacg agatttcctg tgatacaggc cttgccaagc cagttcttta caatagactt
     3301 tcaaggggta ggaagaaaat gcgggaagct tttagtctaa aaggaggttc gagtcaatga
     3361 aggattgtaa caatgtgtac gaactgttaa actacatgga aattgacctg gatgattatg
     3421 aaaaagagat gttaagcgat aaagaaaagc ggagtttaaa aggtctgttt aagaaaaaaa
     3481 gaaggcaaag ggttttaatg caaatcggaa caattgctgc tattttcttt ttagcaattg
     3541 ggtttttcag ccaaacgaat ttgggaaaaa acgtttatgc tgccgtgcaa ttaaaagttg
     3601 cggagatttc ctattccatc ggcgtatcta tgggcataga gagggacatt gagccttatg
     3661 caaatgtggt agatcaaata gtggcagaca atggaataga agtgaaattg agcagtgtca
     3721 taatagataa ggatgaacta atcttctcta taattgtcaa tacgaataaa gctgtggata
     3781 tgttcagatt tgattacgac attttcatca atggtaaaaa actcaccaac tatggtgcca
     3841 ccggtagagc cgggagtata accgattccg acagtttgtt tttccatacg caggcagttg
     3901 atgttgaagg aattgactta aaagatgata tagatgtaag gattgtttta aaagacatgg
     3961 aactgattac aggaacttct caggaaaaga gaaaagggaa gtggcagttt gattttacag
     4021 caaatggcag tgagttaacg gccaactcac gtacgatacc aattgattat tcgtttaata
     4081 tagacaatca ggagtacatt ctacaggaat ttaggtacaa tcctgtaaat caaaagattt
     4141 atggaaaagt aaaaggtgca tttggagcta g
//
LOCUS       DAONUO010000182         4163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15098_length_4163_cov_3.697420, whole genome shotgun sequence.
ACCESSION   DAONUO010000182 DAONUO010000000
VERSION     DAONUO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15098_length_4163_cov_3.697420"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1504)
                     /gene="flgK"
                     /locus_tag="PK768_07110"
     CDS             complement(113..1504)
                     /gene="flgK"
                     /locus_tag="PK768_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HQA60776.1"
                     /translation="MSSFFGIEIAKKALFANQRAQQTVSHNISNANTPGYSRQRVMLE
                     STYTSYGFGAAYQLGTGVKLSDISRIRDDFTDRQYRNENSSLGQWDIQSDILKQVEAV
                     FNEPSDIGISSVLNEFWKSLETLSKDPSSQEARETVKERGITLTNTINHAFIQLNDII
                     NDINYRISVKVNEINSIANQIAQLNLQIQQTQIGGASAADLMDKRDLLLDELSNLVQF
                     ESYEDENGQFTINVGGALLVKGTDTATIEFDTSVADGKITWKEYNSEVRILKGELKGL
                     LDLRDDKIKSYIEQLSTFIQTFATEFNNIHKTAFDLEGNPGEDFFIYDEIQERWSVNP
                     EIVKNPSKIAAAEDENGVPGDNRKALELADLRNKIIDIGGRKSTIDEYYGSLISKLGV
                     DSQEATRAADSQAFMVSQLDERRQMTSSVSLDEEMTKMIQYLHAFNAASRMITAVDEM
                     LNTVINRMGITGR"
     gene            complement(1519..2019)
                     /locus_tag="PK768_07115"
     CDS             complement(1519..2019)
                     /locus_tag="PK768_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HQA60777.1"
                     /translation="MGYREIASVLIEVMQEQLDAYKELYDMGNKKTDVLVNGDIKTLS
                     EITEQEQKLIIKLGKLEDERFELVKEIAKLCNKDASELKGDFIISILDEKEAKTFTAI
                     YDELKILLHDIQEQNDRNERLIKNALDYINFSIKLITDAGTSKANYGADGASSQKSVH
                     FIDKQA"
     gene            complement(2040..2333)
                     /gene="flgM"
                     /locus_tag="PK768_07120"
     CDS             complement(2040..2333)
                     /gene="flgM"
                     /locus_tag="PK768_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HQA60778.1"
                     /translation="MNISRTQLDGIMRAYQKNMESKDKLGKKTPKAQADEVSISDDVR
                     EFANAIKLVSKVEDVRTEKVEELKAKIKAGTYNVDGKLVADKIIEEIFTDKLI"
     gene            complement(2466..2879)
                     /locus_tag="PK768_07125"
     CDS             complement(2466..2879)
                     /locus_tag="PK768_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQA60779.1"
                     /translation="MDLRNCPECGKLFVYVHRNLCPDCLKKDEEDFERVRDFINSNPK
                     ATIEEVSEGTEVPVKKILEHLKEGRLILQSNNVNIILKCEICGESIVTGRICESCGKK
                     LKRNFLGSDKRSMFVDEDMKGKIHLSKYSRDERRR"
     gene            complement(2922..3644)
                     /locus_tag="PK768_07130"
     CDS             complement(2922..3644)
                     /locus_tag="PK768_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQA60780.1"
                     /translation="MIKSIRISDFFLDLLFPRKPYCIICSGKLTPGSHIICESCRDKI
                     IPLSEPMCGKCGKPRHGQSKGTLCRDCQEDYHAFVQARSCGHYEGVLKQLIHEFKYYG
                     KQELAKVLGGMMFDLLKALPWPNFDYIVPLPLHPRRQRERGYNQAYLLARVVASQSGI
                     PIYPVLIRVKATEHQTLLDKTLRKQNLKGAFKVVNGDKIRDKTLLLIDDVYTTGATSG
                     ECSKSLLEAGASAVYVLTCARG"
     gene            complement(3812..4045)
                     /locus_tag="PK768_07135"
     CDS             complement(3812..4045)
                     /locus_tag="PK768_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQA60781.1"
                     /translation="MILKSATIQLEALACPSCMQKIESALKALGGVEQDTVKILFNAS
                     KVKLDFDEEKVSIEEIEDSIKKLGYEVIKSKVK"
BASE COUNT         1023 a          933 c          772 g         1435 t
ORIGIN      
        1 ttctttgggc ggaaatgtta tcgtttatat ctgccaggcg ctttaagttt tgtgacaggt
       61 ttctattgaa attttcaatt atcatggatt gagttattct catgttttat ccctaccttc
      121 cggtaatacc catgcggttg attacagtat ttagcatctc atcgactgcg gttatcattc
      181 ggctggctgc attaaacgca tggagatatt gtatcatctt tgtcatttct tcatccaggg
      241 aaacactgga ggtcatctgc cgcctttcgt caagctggct caccataaaa gcttggctat
      301 cggctgctcg agtggcctcc tgggagtcca cgcctaattt ggatattagg gaaccgtagt
      361 attcgtcaat ggtgctcttt ctgccaccta tatcaataat cttgtttctc aaatccgcca
      421 gttccaatgc ttttctatta tcaccgggaa cgccgttttc atcttctgca gcagctattt
      481 tagatggatt tttcactatt tcaggattta ccgaccatct ttcctggatc tcatcgtaaa
      541 taaagaaatc ttcgcccgga ttgccttcta agtcgaacgc agttttatgg atattgttaa
      601 attctgttgc aaaggtttga atgaaggtgc tcaattgctc tatgtaactt ttaatcttat
      661 catctcttaa atccagcagg cctttcagtt cgccttttaa tattcgtact tctgaattgt
      721 attccttcca tgtgatttta ccatctgcaa ctgatgtgtc gaattcaata gtggcagtgt
      781 cggtcccttt gactaaaagg gcaccgccga cattgatggt aaactggccg ttttcatctt
      841 cataagactc aaactgcacc aagtttgaca gctcgtcaag cagaaggtct cttttgtcca
      901 taaggtctgc cgcagaagct ccgccaattt gggtctgctg tatctgcagg ttcagctgtg
      961 ctatctgatt tgctatgctg tttatttcgt taaccttgac tgatatcctg taattgatgt
     1021 catttattat gtcatttagc tggataaagg cgtgatttat agtgttggtt aaggtgatac
     1081 ccctctcttt gacagtttcc cgggcttcct gggaagacgg atctttggaa agggtctcaa
     1141 gactcttcca aaactcattt aaaactgagg agattccgat atcagaaggc tcattgaaga
     1201 cagcttccac ctgttttaag atatcgctct gaatgtccca ctggcctagg gaagaatttt
     1261 cattccggta ttgcctatcg gtgaaatcat cccgaattcg acttatatca gataatttta
     1321 cgcccgtgcc caattggtag gcggcaccaa aaccatagga agtataggtg gactcgagca
     1381 tcaccctctg ccgcgagtag cccggcgtat tggcattgga tatgttatgt gacactgtct
     1441 gttgggcacg ctggttggca aaaagtgcct tttttgcaat ctctattcca aaaaaactgg
     1501 acaatatact tcaccgcctc aagcttgttt gtcaataaaa tgaaccgatt tctgagaact
     1561 ggcgccatcg gcaccgtagt ttgctttact cgttccggca tcggttatga gcttaattga
     1621 aaaatttata taatccaagg catttttaat cagtctttca tttctgtcat tctgttcttg
     1681 aatatcatgt aaaagtattt ttaattcgtc ataaatagca gtaaaagttt tggcctcttt
     1741 ctcatctaat atggatataa taaaatctcc ttttaactcg gaggcatctt tgttgcacag
     1801 cttggcaatc tcttttacta attcaaatct ttcgtcttca agcttgccca atttaataat
     1861 gagcttttgt tcttgttcgg ttatttcgct aagggtcttg atatcaccgt tgaccagcac
     1921 atcagttttt ttgttgccca tatcatataa ttctttatat gcatcaagct gttcttgcat
     1981 gacctctatt aaaactgatg caatttccct ataccccact tttatagccc cctaattgct
     2041 taaataagct tatcggtaaa aatctcttcg ataattttgt ctgcaaccaa tttgccgtca
     2101 acattatagg ttccggcttt tatctttgct ttcaattctt ctactttttc agtcctgaca
     2161 tcttcgactt tggaaaccaa cttgatggca ttggcaaatt cgcgcacgtc atcagaaata
     2221 ctaacttcat cagcctgtgc tttcggtgtt tttttgccca gtttatcctt agactccatg
     2281 tttttctgat aggctctcat gattccatcg agttgagttc tggagatatt catacaaagt
     2341 gccccctcgc aattagcttc tatatactat atcggactat ccctttatgt ttataaatag
     2401 ctaatatact tttgtactta attcaagttg atttatctct caattgctct acattataac
     2461 ggtacttacc ttcttctttc atcccttgaa tattttgaca ggtgaatttt tcccttcata
     2521 tcttcatcta cgaacataga tcttttatca ctgcctaaaa aatttctttt gagcttttta
     2581 ccgcagcttt cgcaaattct gccggtcact atggattctc cgcagatctc gcacttcaga
     2641 atgatgttga cattgttgct ttgcaaaatt aaacggcctt ctttcaggtg ttccaagatt
     2701 tttttaacgg gaacttccgt gccttccgaa acttcctcaa tggttgcctt gggattactg
     2761 ttgataaagt ctctaacccg ctcaaaatct tcttcgtctt tttttaaaca gtcggggcat
     2821 aggttccgat ggacataaac aaatagtttg ccgcactccg gacaatttct aaggtccatt
     2881 tcactccacc tttccctaaa tatatattaa gttgttcgca actatcctct agcgcaggtc
     2941 aatacgtaga ccgcactggc tccagcttca aggagacttt tggagcactc tcccgatgtt
     3001 gcgcccgtag tgtaaacgtc atcgattaaa agcaatgttt tgtcgcggat tttgtcaccg
     3061 ttgactactt taaaagctcc tttgaggttc tgctttcgca aagttttatc aagtagggtc
     3121 tggtgctccg ttgctttaac cctgattaaa acagggtaaa tggggatacc gctctgactt
     3181 gctacgactc ttgccaacag atacgcctgg ttgtatcccc tctccctttg tcgccttggg
     3241 tgaaggggca gtggcacgat atagtcaaag tttggccagg gaagggcttt taacaggtca
     3301 aacatcatac cgcccaaaac ttttgcaagc tcctgcttcc catagtattt gaattcgtga
     3361 atcagctgtt taagtacgcc ttcataatgg ccgcatgagc gagcctgcac aaaagcatgg
     3421 taatcttcct gacagtcacg gcacagcgta cctttgcttt ggccatgtcg cggtttgccg
     3481 catttcccgc acatgggttc gctcaatggt attatcttgt ctcggcagct ttcacatata
     3541 atatgggatc caggtgtaag cttgccgctg catataatgc agtaaggctt tcgtggaaat
     3601 aggagatcta agaagaaatc tgatatacga atgcttttaa tcatattgac tcacgacatt
     3661 ttattacaac atttaaaacc tatgtttata tttctacagg cttttccaaa ttcctccttt
     3721 tgaggagttc ttttattcac tacttatgaa gtatgtctga aaatatagaa taaaccctgt
     3781 aatccggtga ctacagggtt tatagctttt gtcactttac ctttgatttt atgacttcgt
     3841 aaccgagctt cttaattgaa tcttcgattt cttcaatcga cactttctct tcatcaaagt
     3901 caagcttaac tttgcttgcg ttaaagagta tttttaccgt atcctgctct acgcccccta
     3961 aggctttaag ggcagattcg attttttgca tgcaggaagg acatgccaaa gcttccagtt
     4021 gtatcgttgc cgatttcaag atcatcatct cctctcataa attttctctt ggcgactgtt
     4081 gtatcctaat tatacacttt aatcgtgttt atctgaagcg ataacgcaaa agcctcatgc
     4141 cgtttagaat cactgccaag atg
//
LOCUS       DAONUO010000183         4152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15149_length_4152_cov_3.288504, whole genome shotgun sequence.
ACCESSION   DAONUO010000183 DAONUO010000000
VERSION     DAONUO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15149_length_4152_cov_3.288504"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..747
                     /gene="nth"
                     /locus_tag="PK768_07140"
     CDS             76..747
                     /gene="nth"
                     /locus_tag="PK768_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQA60782.1"
                     /translation="MERDRGDYDNREQARITRILNKLSQLYPEAGTALKYNNPFQLLI
                     ATILAAQCTDKRVNKVTERLFEKYKGPEDFAVADRRELEEDIRECGLFRNKSKNIIAT
                     SRILLEKHGGEVPSSFDELTKLPGVGRKTANVILANAFAIPAFAVDTNVYRLARRLGF
                     SPKKDVYGVEKDLNEKIPESLWIKAHHWLIYHGRNVCRARNPLCDICTLKEDCPEYTK
                     IHSTQ"
     gene            complement(741..1301)
                     /locus_tag="PK768_07145"
     CDS             complement(741..1301)
                     /locus_tag="PK768_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HQA60783.1"
                     /translation="MYKIVENAYFLWGLFLINLFGSIYGFYWYGQQLSETPGILKLFV
                     PDSPTASLLFTLALFMTLIKKPKPFLILMACGWLIKYGLWAAIINTHYYIIGGNYTFT
                     NFHLTLSHLGMAAEGLLFIRGVDYNKYHLFFLICLMIVSDVLDYKLGIHPWLFHQSQL
                     KVAIISIALLTASISLYFIFLYKRRY"
     gene            1446..1886
                     /locus_tag="PK768_07150"
     CDS             1446..1886
                     /locus_tag="PK768_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQA60784.1"
                     /translation="MAKGRFYIVKEEIMPDIFKKTIKAKELLKSGKAKTVNEATEAVG
                     MSRSAYYKYKDYIFPFYEASMGKIVTISFVLEHMPGVLSSILDEIAKARGNILTINQN
                     IPVQNMANVTISFETGNLNKNVEELLAIINKKDCVHKLEIIAQE"
     gene            1902..3173
                     /locus_tag="PK768_07155"
     CDS             1902..3173
                     /locus_tag="PK768_07155"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQA60785.1"
                     /translation="MQIGLLGLGTVGIGVVELLENNKDVIAKRINDHINVKKVLVKDL
                     NKKRTPLVEGKITDKFEDILNDDNISVIVEVMGGEEPALSYIKEALKRGKHVVTANKE
                     VIAKHGRELFELSAENDVNLFFEASVAGGIPIIKPMKECLSANKIVKIMGILNGTTNY
                     ILSQMTEQEKSFDEALKEAQLEGYAESDPTDDIKGYDAARKLAILSSIAFNTRITSDQ
                     VYTEGIDSVSKADIQYAREMGQVIKLIALGKKHEDSVEVSVTPVLLPKDHPLASVSGS
                     FNAIMAEGNAVGPVMFYGLGAGKMPTASAVVADIMETVRNNNCSKFNCTCFDNLEVMD
                     PGQACSSFYIRLLVVDKPGVLAKIAGAFSQYQISLSKVFQKNTINGTAEIVIVTYDVP
                     FENLKRALDQMKDYDEVEEISTVIRVEGEQN"
     gene            3170..>4152
                     /gene="thrC"
                     /locus_tag="PK768_07160"
     CDS             3170..>4152
                     /gene="thrC"
                     /locus_tag="PK768_07160"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQA60786.1"
                     /translation="MSVKTGVIDNYFSYLPVKDRKNIISLNEGNTPLIRARNIEELLD
                     TKAEIYLKYEGLNPTGSFKDRGMTVAVSKAVEDGASAVICASTGNTSASAAAYAAKAC
                     LKCVVLIPEGAIAMGKLAQAIAYGAKVIAIRGNFDDALKMVRELAERRPITLVNSINP
                     HRIEGQKTASFEICDVLKEAPDYLAIPVGNAGNITAYWKGFKEYYSLKKIGKLPKMMG
                     FQAAGAAPIVENRIIPNPETIATAIRIGNPASWKYAVEAAQSSQGKIDKVTDKEILEA
                     YKILAKYEGIFVEPASAASVAGVIKMCKKNIINDGEKVVAVLTGHGLKDPDT"
BASE COUNT         1407 a          741 c          947 g         1057 t
ORIGIN      
        1 aaaagctata gatcttttat tggatatatt agaacgtaaa aatgctgcat aacaagttgt
       61 gtgaggaagg tatttatgga aagggataga ggagactacg ataacagaga acaggcgagg
      121 ataacacgaa ttcttaataa gctgagtcaa ttatacccgg aagctggcac agctcttaag
      181 tacaacaacc cctttcagct tttgattgcc actattttgg ctgcccaatg cactgacaaa
      241 agggttaaca aggttacgga aaggctcttt gagaaataca aagggcctga agattttgca
      301 gttgcagata ggcgagaact tgaagaagat ataagggaat gcggcctttt caggaataaa
      361 agcaagaaca tcatagcaac gtccagaata ttattggaaa aacatggggg tgaggtgcct
      421 tcttcctttg atgaattgac aaaacttcca ggtgttggcc gaaaaactgc taatgtgatt
      481 ttggccaatg cctttgctat accagctttt gccgtggata ccaacgtata tagattggct
      541 cgtcgtttgg gcttttcgcc aaaaaaagat gtctatggtg ttgagaagga tttaaacgaa
      601 aaaatacccg aatctctttg gataaaggca caccattggt taatatacca tggtcgcaat
      661 gtttgccgcg ctcgaaatcc tctgtgtgat atatgcactt taaaagaaga ttgtccggag
      721 tatacaaaaa tccacagcac tcagtagcgc cttttgtaca aaaagataaa gtaaagacta
      781 atggaagcgg tcagcaaagc aatagaaata atggcaactt tcagttgact ttggtgaaaa
      841 agccatgggt gtatgccaag cttataatct aagacgtcac ttacaatcat gaggcaaatt
      901 aagaaaaaca agtggtattt attgtaatca acacccctta taaaaagcaa accttctgcc
      961 gccatgccaa ggtgagatag tgtcagatga aagttcgtaa acgtgtaatt gccaccgatt
     1021 atataataat gggtattgat aattgctgcc caaagtccat acttgatcaa ccagccgcag
     1081 gccatcagta ttaaaaatgg cttgggcttt tttattagcg tcataaaaag ggccagagta
     1141 aaaagcaagg aggctgttgg gctgtcgggg acaaacagct ttaaaatacc cggtgtctct
     1201 gacaattgct gaccgtacca ataaaacccg tatatggagc cgaaaaggtt tattaaaaat
     1261 agcccccaaa gaaaatacgc attttcgaca attttataca tgagcatccc tccttaacac
     1321 attgtaaaga cattataaca taaaaattat ctattgtttt ttagctaatg ttatgctata
     1381 atgtcttcca tacagaaata gttgtatgtc ttgcataaac aatgcaaagg aggatttgac
     1441 ttttggtggc gaagggcagg ttttacatag taaaagaaga aataatgcct gacatcttta
     1501 aaaaaaccat caaagctaaa gagcttttga aaagtgggaa ggccaaaacg gtcaatgagg
     1561 cgacagaggc tgtgggtatg agcagaagcg cctattacaa gtacaaggac tatatttttc
     1621 ctttttacga ggcaagtatg ggcaaaatcg tgacaatatc atttgttcta gaacacatgc
     1681 ctggcgtttt gtccagtatt ttggacgaaa ttgcaaaggc tcggggaaat atattgacca
     1741 tcaatcagaa catacctgtg caaaatatgg cgaatgtcac catctccttt gaaaccggaa
     1801 atctgaacaa aaatgttgaa gaattgcttg cgataataaa caaaaaagat tgcgtgcaca
     1861 aacttgagat aattgcacaa gaatgagagg agagttataa tatgcaaatc ggactattgg
     1921 gtttaggcac agtcggaata ggcgttgttg aattattgga aaacaacaaa gacgtaatag
     1981 caaagaggat aaatgaccac ataaatgtca aaaaagtact tgtaaaggat ttaaataaaa
     2041 agaggactcc cctggtggag gggaagataa ccgataaatt cgaagatatt ttaaatgatg
     2101 ataatatttc agtcattgta gaggtgatgg gaggggaaga acctgcctta tcttatatca
     2161 aggaagcttt aaaacggggc aaacacgtgg taacggcaaa taaggaggtc atagcgaaac
     2221 acggcagaga actctttgag ctctcggcag aaaatgatgt caatttgttt tttgaggcaa
     2281 gtgtggccgg cggcattccc attataaagc ctatgaaaga atgcttgtca gcaaataaaa
     2341 tagtaaaaat aatggggata ctaaacggta caacaaatta catcttaagt caaatgaccg
     2401 aacaggaaaa aagttttgac gaagccttga aagaagcaca actggaaggc tatgctgaaa
     2461 gcgatcccac cgatgatatt aaaggatatg atgcagcccg aaagcttgcg atactatcat
     2521 ccatagcttt taatactcga ataacttccg atcaggtcta taccgaaggt attgatagtg
     2581 taagtaaagc tgatatacaa tatgcccgtg agatggggca agttataaag ctaatagctc
     2641 tcggcaaaaa gcatgaagat agcgtagaag tttctgtgac gccggtatta ttgccaaaag
     2701 accatccctt ggccagtgta tcgggctcct ttaatgctat catggctgaa ggaaatgccg
     2761 ttggtcctgt catgttttat ggtttaggtg cagggaaaat gcctactgca agtgctgtag
     2821 tggcagatat catggagacg gttagaaata acaattgtag caagttcaac tgcacctgtt
     2881 ttgacaacct ggaagtgatg gaccctggcc aggcttgttc aagtttttac attagacttc
     2941 tagttgtcga caaacccggt gtacttgcaa aaatagccgg tgcctttagc cagtatcaaa
     3001 tcagcctgtc aaaagtattt caaaaaaaca ccataaatgg cacagccgaa atcgtcattg
     3061 tcacttacga tgtgcccttt gaaaatctca aaagggcact tgaccaaatg aaagattatg
     3121 atgaggtgga agagatatcg accgtcatca gagttgaggg ggaacaaaat tgagtgtaaa
     3181 gacaggagta attgacaatt acttttcata ccttcctgtt aaagatagga aaaacattat
     3241 ttccttaaac gaagggaata ctccgcttat aagagccaga aatatcgagg agttattaga
     3301 tacgaaggca gaaatctatt tgaaatatga aggcctcaat cctacgggtt cttttaaaga
     3361 cagagggatg acggtggcag tatccaaggc ggtggaggac ggtgcctccg ctgtaatatg
     3421 tgcctcaacg ggcaacactt cagcctccgc agcagcctat gcggcaaaag cctgcttaaa
     3481 atgcgtcgtt ctaatccccg aaggtgctat cgccatgggc aaactagcac aggcgattgc
     3541 atatggtgca aaggttatcg ccattagagg taattttgac gatgccttaa aaatggtgag
     3601 agagcttgcc gagcgaaggc ctatcactct cgtaaactct attaatccac acagaataga
     3661 ggggcaaaaa actgcttcat ttgaaatatg cgatgtgctt aaggaagctc cggactatct
     3721 tgctataccg gtaggaaatg cagggaatat tacggcatat tggaagggct ttaaggaata
     3781 ttattcactt aaaaagatcg gcaaactgcc taaaatgatg ggctttcagg ctgccggtgc
     3841 agcaccaatt gtagaaaata gaataattcc aaacccagag actattgcca cagctattcg
     3901 cattggaaac ccggccagtt ggaaatatgc ggtggaggca gctcaaagct ctcaaggcaa
     3961 aatagacaag gtgacggata aagaaattct tgaagcatat aagatacttg ctaaatatga
     4021 aggtattttt gttgagccgg cttcggcagc ttccgtagca ggagtaataa agatgtgtaa
     4081 gaaaaatata attaatgatg gagaaaaagt tgtggcagtg cttaccggtc acggcttaaa
     4141 agaccccgat ac
//
LOCUS       DAONUO010000184         4124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15286_length_4124_cov_3.695011, whole genome shotgun sequence.
ACCESSION   DAONUO010000184 DAONUO010000000
VERSION     DAONUO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15286_length_4124_cov_3.695011"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK768_07165"
     CDS             <1..192
                     /locus_tag="PK768_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQA60787.1"
                     /translation="TNKDPVTVPDLTGRTIKEATDIIHAIGLNIEIEGSGYAVKQNPP
                     AEAEVGLGTTVKVEFSQKP"
     gene            259..1728
                     /locus_tag="PK768_07170"
     CDS             259..1728
                     /locus_tag="PK768_07170"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQA60788.1"
                     /translation="MNICELIKILPGLISVEGPLDVKVNSVVDDSRCAKNGALFVAVT
                     GENFDGHDFIHDAIKFGAKAVIGEKSLHFTNDITYIRVKNSRKALAKSSAWFYGFPAD
                     KLKLIGVTGTSGKTTTTYLIRAMLNEVGARSGLIGTIANVINDKQLPTSFTTPGALEL
                     NQLFLQMLEHNVDYVVMEVSSHSLKLHRVEGLQFQVGVFTNLTRDHLDFHKSFEDYFS
                     SKQKLFQQSKRAVINIDDDSGKKLLDAINVPALTFGIENSADLIAQNVRQSSKGVFYD
                     LIYKDKKYSVSYGVPGLFSVYNSLAALAAGISLGFAMDSLIKALQNVEGVPGRFELIK
                     NDRGIIVIVDYAHKPDGLKNVLYTMREFSKGRIITVFGCGGDRDRGKRPIMGKISTEL
                     SDFTIITSDNPRGEDPEAIIREIEKGAVGKNYMKIPDRQEAIKAALSIAKKGDAVLIA
                     GKGHETYQIIGDKKIHFDDREIAKEFLYGGGASDENIKP"
     gene            1709..3094
                     /gene="murF"
                     /locus_tag="PK768_07175"
     CDS             1709..3094
                     /gene="murF"
                     /locus_tag="PK768_07175"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQA60789.1"
                     /translation="MKTLNLREVIEATRGKYSGKHFDILIKGVSTDSRTIRENDLFIP
                     LEGPNFDGHDFIRQAFEKGATAVLCEESKREKVKALPGDKVIFVDNTQLALLDLAAYY
                     RALFPIPFVAVTGSVGKTTTKEMIAEVLKSRFRVLKTPGNLNNEIGVPLTMFLLDECH
                     DIGVVEMGMSGLGEIRRLANVVKPKVAVITNIGVTHIEKLGSREKIAQAKMEILEPLT
                     GKDIAVLNADSAELWRAKEGIVPKTVFFGREAGDIRAKNIVSDDDEIKFDVYGCYGEA
                     TFTVSLPGVHNVNNALAAIAVGFEMGLSKEEIQAGLKKLKLPEMRLQLKQSFFGAEVI
                     DDAYNASPDSMKAALDYLEQRGRDKKKAAILGDMLELGNIASSAHFEIGKYAAKKADM
                     IVAIGSFSEDIKKGALEGKIDVNCVHAFKALEQASAEIIGLVEDCDIVLVKASRGMKL
                     EQITELLVRGS"
     gene            3102..4046
                     /gene="mraY"
                     /locus_tag="PK768_07180"
     CDS             3102..4046
                     /gene="mraY"
                     /locus_tag="PK768_07180"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQA60790.1"
                     /translation="MQFITALVSFIITAVLGILLIPVLDKLKFGQRVRDDGPQSHLKK
                     MGTPTMGGIIFIPAIALATLLFSDINVDTVALIFIITGFGLIGFADDFIKISKKRSLG
                     LRANQKLLFQLLLSAGLMIYIFAYYAKPTEIIIPLHHLRLDLGLFYIPFTVFLILGTV
                     NSVNLTDGLDGLVSGIIAIIAFFYTALLAQSNLNLAIFSSAVTGGCLGFLLFNRHPAR
                     VFMGDTGSLGLGGALAAITVLSRSHLFLAIIGIIFIIETLSVILQVLSFKFTGKRILK
                     MSPIHHHFELCGWSERKVVHTFWLITLIAGILALMMFM"
BASE COUNT         1277 a          748 c          988 g         1111 t
ORIGIN      
        1 acaaataaag atcccgtgac tgtccctgat ttgacgggca gaacaatcaa agaggctacc
       61 gacatcatac atgccatcgg cctaaacatc gaaatagaag ggagcgggta tgcagtaaaa
      121 caaaatccac cggcagaagc agaagtaggt ttagggacta cagtcaaagt tgagttttct
      181 cagaaaccat gagatttgaa attagattca gcaacagtta aaatagattt gacatcagaa
      241 taaaaaaaga gggttactat gaatatctgt gaattaataa agattctacc tggtctaatt
      301 tctgtagaag gtcctctgga tgtgaaagta aattcggtag tggatgattc aagatgcgca
      361 aagaatgggg cgctctttgt ggcagtgacc ggcgagaatt ttgatggcca cgattttata
      421 catgatgcaa taaagttcgg agctaaagct gtaattggtg aaaaatcact tcattttaca
      481 aatgatataa cgtatattag ggtgaaaaac agccgaaaag ctttggcaaa aagttctgca
      541 tggttttacg gcttccccgc tgataaatta aagcttatcg gagttacggg aaccagcggc
      601 aaaaccacaa caacctacct aataagggcc atgctaaatg aagtaggggc aaggtcggga
      661 ctgatcggca ccatagccaa cgttataaac gataaacaac ttcctacatc ttttaccact
      721 cccggggctt tggagctaaa tcagctcttt ttacagatgc tggagcataa cgtggattat
      781 gtggttatgg aggtgtcctc tcactcgctg aaacttcatc gggtggaagg cttgcaattt
      841 caagtaggag tatttaccaa tctcacgcga gatcatctgg attttcataa aagctttgaa
      901 gactatttct cttcaaaaca aaaactcttt caacaatcta aacgggcagt aattaatatc
      961 gacgatgata gcggtaagaa gctactcgat gctataaacg ttcccgcatt gactttcggt
     1021 atagaaaatt cagccgactt aatagcacaa aatgtaaggc aaagcagcaa aggggttttt
     1081 tacgatctca tttataaaga taagaagtat tctgtatctt acggtgtgcc cggtcttttt
     1141 agcgtataca actcgctggc agccttagcc gccgggattt ctctcggctt tgccatggac
     1201 agtttaataa aagccttgca gaatgttgaa ggggtgccgg gtaggtttga acttataaaa
     1261 aacgatcggg gcataattgt cattgtggat tatgcccata aaccggacgg cttaaaaaat
     1321 gtgctttata ctatgaggga gtttagcaag gggaggataa ttacagtttt cggatgtggc
     1381 ggagacagag acaggggcaa aaggcctata atgggcaaaa tatcgacaga gctatcagat
     1441 ttcactatta ttacatcgga taatcccagg ggcgaggatc cggaagccat tatccgggaa
     1501 attgaaaagg gagcggttgg caaaaactat atgaaaattc ctgataggca agaggcaatt
     1561 aaagcagctt tgagcatagc taagaaaggc gatgcggtgc tcattgcggg aaagggccat
     1621 gaaacttatc agattatagg cgataagaag atacactttg atgatagaga gatagcaaaa
     1681 gagttcttat atggcggagg agcatcagat gaaaacatta aaccttagag aagtcataga
     1741 ggcaacccga ggcaaatatt caggcaaaca ctttgatata ctaattaaag gcgtctcaac
     1801 ggattccaga acgataagag aaaatgactt gttcatcccc ttggaaggac caaactttga
     1861 cggtcacgat ttcataagac aggcctttga aaaaggggca acagctgtcc tttgcgagga
     1921 aagcaaaagg gagaaagtaa aggctctacc tggcgataaa gttatctttg ttgacaacac
     1981 tcaactggca ttgctagatt tagccgccta ttacagggcg ctttttccca taccctttgt
     2041 tgctgtaacc ggcagtgtag gtaagactac cactaaagag atgattgctg aggtactaaa
     2101 aagcaggttc agagttttaa aaacaccggg aaatttaaac aatgaaatcg gggtccccct
     2161 tactatgttc ctgttggacg aatgtcacga tataggggta gttgagatgg gtatgagcgg
     2221 cctgggagaa atccgcagat tagctaatgt cgtaaaacca aaagtggccg tcataacaaa
     2281 cattggagtg acgcacatag aaaaactggg aagcagggag aaaatcgcgc aggcaaagat
     2341 ggaaatactt gagccactta caggaaaaga catagcggtg ctcaatgctg acagcgcaga
     2401 attgtggagg gcaaaggaag ggattgtacc taaaactgtg ttcttcggca gggaggccgg
     2461 agatatcagg gcaaaaaata ttgtttcgga tgacgatgaa attaagtttg acgtatacgg
     2521 ttgctatgga gaggcgactt ttacggtctc attgccggga gttcacaatg taaataatgc
     2581 tttggcagct atagcagtag gttttgaaat gggtcttagc aaagaagaga tacaggcggg
     2641 acttaaaaag ctgaagctgc cggaaatgag gcttcaattg aagcaatcct tttttggagc
     2701 cgaggtcatt gacgatgcgt acaatgccag cccagattcc atgaaagctg cccttgatta
     2761 tttagaacaa aggggtaggg ataagaaaaa agccgccata ttaggtgata tgctggaatt
     2821 aggcaatatt gcaagcagcg cccattttga gatcggcaag tatgcggcga aaaaagctga
     2881 catgattgtt gccataggca gcttttctga agacattaag aagggtgcat tggaaggcaa
     2941 aattgacgtt aactgtgtcc atgcattcaa agcccttgaa caggcctcag cagagattat
     3001 aggactagtg gaagactgtg atatagttct agtgaaagct tcaagaggta tgaagttgga
     3061 gcagattact gagcttttag tcagggggtc ttaagttggg tgtgcaattt attacagctc
     3121 tagtctcatt tattattacc gctgtattgg gcatattatt aataccggta ttggacaaac
     3181 tcaaatttgg acaaagggtt agagatgatg gtccccagag tcacttgaaa aaaatgggga
     3241 ctcccactat gggcggcatt attttcatcc ccgccatcgc actggcaaca cttttgtttt
     3301 cagatattaa tgtggacact gttgcgctta tttttattat tacgggattt gggctaatag
     3361 gttttgctga tgattttata aagatcagca aaaaacggtc tttaggcctt agagctaatc
     3421 aaaaactact atttcaactt ttgctctctg cgggtctgat gatttatata tttgcatact
     3481 atgctaagcc cacagaaata atcattcccc ttcatcacct aagactcgat cttggattat
     3541 tctacattcc ctttactgtc tttctcatat tgggcacggt aaacagcgta aatcttacag
     3601 atggattaga cggacttgtt tcgggaatca ttgcgataat tgcatttttt tatacggccc
     3661 ttttagcaca aagcaactta aatctggcca tttttagttc tgccgtaacg ggaggatgct
     3721 tggggtttct gttgttcaac cgccatcctg caagggtttt catgggcgac actggttctt
     3781 tgggcttagg tggtgccctg gcagccataa cagtgttatc ccgaagtcat ctttttcttg
     3841 caatcatcgg cattatattc ataatcgaaa ctctttcggt tatcttgcag gtactttctt
     3901 ttaaattcac cggaaaaaga atacttaaga tgagccccat tcaccaccat ttcgaattgt
     3961 gcggatggag cgagcggaag gtagttcata ctttttggtt gattacctta attgcaggta
     4021 ttcttgcttt aatgatgttt atgtagcttt tcgagtaata ccttaggaaa tagagaaagg
     4081 ggaaatcaga aagtggaact gaaggggaaa aacgtgctga tatt
//
LOCUS       DAONUO010000185         4117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15313_length_4117_cov_3.652634, whole genome shotgun sequence.
ACCESSION   DAONUO010000185 DAONUO010000000
VERSION     DAONUO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15313_length_4117_cov_3.652634"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1501)
                     /locus_tag="PK768_07185"
     CDS             complement(149..1501)
                     /locus_tag="PK768_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HQA60791.1"
                     /translation="MKYKNLIIIFMGAMLLLTGCLAVPGNKEAIDKSSLPLDRTTYIG
                     YSDATDHGYAMAVVTLEDDRITDVTLKEFMELSVEKDFSSYEYYPSVQAHTVLPQRFV
                     EAQGTEVDSISGATASSNRYKQAVERALESAKSKEGQNKYFDGIFQGRSKADNHGYGI
                     ALVEIKDDRIVRVELKEVDEKGELKDFSTYPHEPSRQAHRELPERFVQSNSAGIDNFT
                     GATHSTDKYKEAVRNALVKARIEKDLPNLIDGTYLAVSDVSNLGYSSARVTVENSFIV
                     QVDLKEYDQNSVEKDFDLYEYEPSVNANRELPWAFVAAQSPRVDMVTGATYSSKQCIQ
                     AVERAIEKAALIKRAGKYFDGVFQAKSKIDEHGYAIAVVTIKDDQMTNVELKYIDADG
                     RERDFDTYEYEPSVIAYRELPKRFVTANSYDVDTITGATSSSIKYREAVKNALEMARR
                     "
     gene            complement(1593..2045)
                     /locus_tag="PK768_07190"
     CDS             complement(1593..2045)
                     /locus_tag="PK768_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60792.1"
                     /translation="MALREFILKIAGKFKKTRTGVFDENLFRELQERILKLEKRQHEL
                     EKSLKKEPCVDNIVVERLYTEKIELNFDAIDIKELSGMLSIGLNYDGKLVKLNSGEIG
                     ETKEDGKNTNDKKGTKIVQTNNNGSFKDIQAPKPKIKVYFDNQPLSNN"
     gene            2187..2456
                     /locus_tag="PK768_07195"
     CDS             2187..2456
                     /locus_tag="PK768_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60793.1"
                     /translation="MAGLCLKINIGSINIGSIENASSFNIGKNFLREFESMTKTNQGL
                     GNISGGNNTLPGNTNYVEDPDALDMWCGAAAKNEKRLIEELKEIL"
     gene            2478..3005
                     /locus_tag="PK768_07200"
     CDS             2478..3005
                     /locus_tag="PK768_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQA60794.1"
                     /translation="MFDKEWLESLIELQKKSRELAEGVAWPKEYLDAVMQAIQNSSKN
                     PASFGMPDYSREFKGLWNVGTQFVYEDSHEPKISITETAKAFLIKAAIPGLQDKRDIC
                     VKVHGNVVNISGKIPDNSGKQDKKPLMSFNKSIPLPAGIDPQKSTAKYWDGTLTLYLP
                     KSSGTDLQQIEVSFS"
     gene            3044..3286
                     /locus_tag="PK768_07205"
     CDS             3044..3286
                     /locus_tag="PK768_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60795.1"
                     /translation="MTVSIFFNAINVLNMQTSSIVAIGENAQAGWDSHTKANTGCTTH
                     GISLTSANLITIIDPDAIDTPINDQDIKPTGQVQQV"
     gene            3288..3800
                     /locus_tag="PK768_07210"
     CDS             3288..3800
                     /locus_tag="PK768_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60796.1"
                     /translation="MMRMQNKAEDIVVNFQKLTVSSIGPSSGIFIGKNNAIGWSAHGK
                     SNQGLGSVNGNVMHNLNMVFDNDLVDSPIDDRDLIVDSDMYLSAKIEDDARITLDEIN
                     VTSLSGNAAISVGKNRQSGWDAHSKANTGIGQHYDDSITCNNLSVIDDADLIDSPIND
                     QDVKASICKR"
     gene            complement(3826..>4117)
                     /locus_tag="PK768_07215"
     CDS             complement(3826..>4117)
                     /locus_tag="PK768_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HQA60797.1"
                     /translation="GTPGVAEVLPKIARELKGKITILADGGVRSGVDVLKYIALGADA
                     VLVGRPVIIGAFGGGAEGVKLVLETMAKELKQAMILTGCNNIGEISDRVLFN"
BASE COUNT         1113 a          951 c          856 g         1197 t
ORIGIN      
        1 atgcaggcaa gaggtaagga agtcgtcttt ttaaagcgcc tgtccatcgg cccattaaag
       61 ctcgatgaaa cactcggccc cggagactgg cgcgagctta cggaagatga gatttattcg
      121 ttaaatcact ttgacagcat gtaaatcctc agcgtcgggc catttccagg gcatttttca
      181 ccgcttctct gtatttaatg gaactgctgg tggctcctgt gatggtgtca acgtcatagg
      241 aatttgccgt gacaaatctc ttgggaagtt ctctgtaggc gattaccgag ggttcatact
      301 catatgtgtc aaaatccctc tccctaccgt cagcatcgat gtactttaat tctacattgg
      361 tcatttggtc atcctttatg gtgactacgg caattgcgta cccgtgttca tcgatctttg
      421 atttggcttg aaacactccg tcaaagtatt tcccagctct tttaatcaaa gcagccttct
      481 caatagccct ttctaccgcc tgaatgcact gtttgctgct atacgtggcc cccgtcacca
      541 tatcaacacg gggtgattgg gctgccacaa aagcccaggg cagctctcta ttggcattta
      601 ccgaaggctc atactcatat aaatcaaaat ccttttccac agaattttga tcatattcct
      661 ttaaatccac ctgcacaata aaactgtttt ctacggttac cctggcactg gaatatccca
      721 ggtttgacac atccgaaacc gccaggtatg tcccatcgat caaattgggc agatcttttt
      781 ctatccttgc ttttaccaag gcgtttcgca cggcctcttt gtacttatcg gtgctgtggg
      841 tggctccggt aaagttatca atgcctgccg aattgctctg gacaaaacgc tcgggcagtt
      901 ctctgtgagc ctgtctggac ggctcatggg ggtaagtact aaaatcctta agttctccct
      961 tctcgtccac ttctttaagt tccaccctta caatcctatc atctttgatt tcaacaaggg
     1021 caatgccgta gccgtgattg tcggcttttg acctgccctg aaaaatgccg tcaaagtatt
     1081 tgttttgccc ttctttggat ttggcactct ctaatgctct ctccactgcc tgcttatagc
     1141 ggtttgaact ggcggtggca ccgctgatcg aatcaacttc agtaccttgt gcttctacaa
     1201 agcgttgggg cagcacagta tgagcctgaa ccgatggata atattcatag ctggaaaaat
     1261 ctttttccac tgaaagttcc ataaactcct tgagggttac atcggtaatc ctgtcgtctt
     1321 ctagcgtaac tactgccatg gcatagccat ggtccgttgc atctgaatag ccgatatatg
     1381 tcgtcctgtc aaggggaagc gaagatttgt caatagcctc tttatttccg ggcacagcaa
     1441 ggcatccggt aagcagcagc atggcgccca taaaaattat gataagattt ttatatttca
     1501 tagcttgaca cctcatgtgg ccaaaacctt aatattccaa cactagcttg gcctttcaat
     1561 tgaatactaa tatgaatgcg accaaaagtc aatcaattat tggataatgg ctgattgtca
     1621 aaatacacct taattttagg cttcggtgct tgaatatctt tgaaacttcc attgttgttt
     1681 gtctggacaa tttttgttcc tttcttatcg tttgtgtttt tgccatcctc ctttgtttcg
     1741 ccgatttctc cagagtttag ctttaccagt ttcccatcat agtttaagcc tatgctgagc
     1801 atgccgctta gctcttttat atcaatggca tcaaagttca actctatttt ttcggtataa
     1861 agcctttcta ccacaatgtt gtcaacacaa ggctcttttt ttaggctttt ttccaactca
     1921 tgctgccgtt tttccaattt taaaatacgt tcctggagtt ctcgaaacaa gttttcatca
     1981 aagactccgg ttctggtctt cttaaatttg ccggcaattt taagtataaa ctctcttagg
     2041 gccaagagtt tcacctcttg cagctaaatc ttaatcaagg aggtcacatc tcaacagttc
     2101 cgtcataata atatagtatg gtatatatga taaaatacct ggtttttgtc aatgcccgac
     2161 cctcatattt ttttggaggt ttttatatgg cagggttgtg cttgaaaatc aacataggtt
     2221 ccataaatat cggttctatc gaaaatgcct catcttttaa cattggcaag aattttttaa
     2281 gggaatttga aagcatgacc aaaaccaacc agggcttagg taatatctcc ggaggtaata
     2341 acacattacc cggcaatacc aattatgtag aagaccccga tgctctggat atgtggtgcg
     2401 gagctgctgc taagaatgaa aaacggttaa ttgaagagtt aaaagaaatt ctttgatttg
     2461 tccggggggt tgtatgtgtg tttgataaag agtggctgga aagtttaata gagcttcaga
     2521 aaaagtctcg ggagcttgct gaaggagtgg cctggcccaa ggagtacctg gacgcggtga
     2581 tgcaggctat acaaaacagc tcaaaaaacc ccgcctcatt tggcatgccc gattactcaa
     2641 gagagtttaa aggtttatgg aatgttggaa ctcaatttgt ttatgaagac agccatgaac
     2701 ctaaaatcag tattacggaa accgcgaaag catttttgat taaagcagca atacccggtc
     2761 ttcaggacaa aagggatatt tgcgtcaagg tgcatggaaa tgttgtaaac atttccggaa
     2821 aaataccgga taattccgga aaacaagata aaaaaccttt aatgtctttt aacaagagca
     2881 ttcccctgcc ggctggaatt gacccacaaa aatccactgc aaaatactgg gatggaacac
     2941 taaccctgta tttacctaaa agttcaggga ctgacctgca gcaaattgaa gtcagcttct
     3001 cttaaagtat agaaaattgc ctgtaaagaa ttgagggatt gccatgaccg tgtccatttt
     3061 tttcaatgca ataaacgtcc tcaacatgca gactagttcg atcgtggcta taggggaaaa
     3121 tgcccaggcc ggctgggatt cccacaccaa agccaatacc ggctgcacta ctcacgggat
     3181 aagcttaact tcagccaatt taattacgat catcgaccct gatgccattg acaccccaat
     3241 caacgaccag gatattaagc ctaccggcca ggttcagcag gtctgaggtg atgaggatgc
     3301 agaataaggc agaagacatt gttgtaaact ttcaaaagct taccgtctca tctatcgggc
     3361 catccagcgg gatttttata gggaaaaaca atgccatagg ctggagcgcc cacggcaaaa
     3421 gtaatcaggg tttgggttct gtaaatggca atgtcatgca taacctaaat atggtttttg
     3481 ataatgattt ggtggattca cccattgatg accgagattt gatagttgac agcgatatgt
     3541 atctttcggc aaaaatagaa gacgatgcga gaataacatt agacgaaatc aatgtcacat
     3601 ccctttccgg caatgctgcc atatctgtag ggaaaaacag gcaaagcggc tgggatgccc
     3661 acagcaaggc caataccggt atcggccaac actacgatga cagcatcacc tgcaataacc
     3721 taagtgttat tgacgatgct gacctgattg attcaccgat taacgaccag gatgttaaag
     3781 caagtatatg taaaaggtga cagatttttt ctgtcacctg ggctcctagt taaataaaac
     3841 tctatcactt atttcgccga tattgttgca gccggtcaga atcatggcct gttttagttc
     3901 cttcgccatg gtttccagca cgagcttcac cccttcggct cctccgccaa aggcaccgat
     3961 aattacgggt ctgcccacca gcaccgcatc ggcgcccaat gctatgtatt ttaaaacatc
     4021 cacaccgctt ctgactcctc cgtcagccaa aatagtgatc ttgcccttca gctcccgggc
     4081 aattttcggc aggacctctg ccacacccgg tgtgccg
//
LOCUS       DAONUO010000186         4110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15336_length_4110_cov_4.001233, whole genome shotgun sequence.
ACCESSION   DAONUO010000186 DAONUO010000000
VERSION     DAONUO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15336_length_4110_cov_4.001233"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="rpsC"
                     /locus_tag="PK768_07220"
     CDS             complement(<1..613)
                     /gene="rpsC"
                     /locus_tag="PK768_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQA60798.1"
                     /translation="MGQKIHPHGLRVGIIKDWDSRWYSEKDFADQLLEDYKIRQYIKK
                     KLYQAGISRIEIERAAKRIRVTVNTAKPGMVIGKGGSGVDELKAELEQMTDKQVSINV
                     VEIKNPDIDAQLVAENIASQLERRISFRRAMKQAISRALKSGAKGIKTMVSGRLGGAE
                     IARSEMYHEGTIPLQTLRADIDYGFAEAHTTYGRIGVKVWIYKG"
     gene            complement(617..955)
                     /gene="rplV"
                     /locus_tag="PK768_07225"
     CDS             complement(617..955)
                     /gene="rplV"
                     /locus_tag="PK768_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQA60799.1"
                     /translation="MEARARARYVRISPSKVRVVLDLIRGKHVDEALTILRFTPKAAS
                     PLLEKLLRSAIANAENNFDMNRDALYISQCYADQGPIMKRYRPRAQGRVYSIHKKTSH
                     ITIVLKEKEG"
     gene            complement(991..1275)
                     /gene="rpsS"
                     /locus_tag="PK768_07230"
     CDS             complement(991..1275)
                     /gene="rpsS"
                     /locus_tag="PK768_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQA60800.1"
                     /translation="MSRSTKKGPFVDKKLLARIVEMNKKREKKVIKTWSRASTIFPEM
                     VGHTIAVHDGRKHVPVYITEDMVGHKLGEFAPTRTFRGHGSHTERSTALK"
     gene            complement(1291..2118)
                     /gene="rplB"
                     /locus_tag="PK768_07235"
     CDS             complement(1291..2118)
                     /gene="rplB"
                     /locus_tag="PK768_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQA60801.1"
                     /translation="MAIKKFKPTTPGRRFMSVPTFEEITKKKPERSLVEILKNKAGRN
                     MQGRMTMRHRGGGHKRKYRIIDFKRDKDGIPAKVAAIEYDPNRTAYIALLHYADGEKR
                     YILAPDGLRVGDTVMSGENADIRPGNALKLVDIPVGSTVHNIELVPGKGGQMVRAAGT
                     SAQLMAREGGMATLRLPSGEMRMVPEECRATIGQVGNIDHENITIGKAGRSRWLNRRP
                     HVRGVVMNPVDHPHGGGEGKAPIGRKSPVTPWGKPTLGYKTRKKNKASDKYIVKRRK"
     gene            complement(2168..2458)
                     /gene="rplW"
                     /locus_tag="PK768_07240"
     CDS             complement(2168..2458)
                     /gene="rplW"
                     /locus_tag="PK768_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQA60802.1"
                     /translation="MKHPHDIIIRPWITEKSMDLQQDRKYVFVVDKNSNKTEIKNAVE
                     AIFGVKVAKVNTINMRGKMKRMGRFEGKRPDWKKAIVTLTEDSKTIEFFEGL"
     gene            complement(2455..3081)
                     /gene="rplD"
                     /locus_tag="PK768_07245"
     CDS             complement(2455..3081)
                     /gene="rplD"
                     /locus_tag="PK768_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQA60803.1"
                     /translation="MPKVALYNIQGDKVGEIELSDEVFGVEIRPDIMHRAVLSYLANQ
                     RLGTASTLTRAEVSGGGRKPWRQKGTGRARHGSIRSPIWRKGGIVFGPKPRSFSMKMP
                     KKLKRFALKSALSAKTKENELILLDSLTMEAPKTKQMVSILSNLKVDKKSLIVIADKD
                     ENVVKSARNIPGVKTTYVNTLNVYDILNHDYLIMTQEAAKRVEEVYAG"
     gene            complement(3101..3736)
                     /gene="rplC"
                     /locus_tag="PK768_07250"
     CDS             complement(3101..3736)
                     /gene="rplC"
                     /locus_tag="PK768_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQA60804.1"
                     /translation="MISKAILGKKIGMTQIFSDTGESLPVTLVQAGPCIVVRKRTLEN
                     DGYSAIQVGYEDIKEKKLNKPLQGQFKKSQVPFKRYLKEFKIKDADKYNVGDEIKVDI
                     FQPGDKVDVTGISKGKGFAGTIKRWNFRRGPMSHGSKSHRIVGSIGAAGVGKVVKGLK
                     AAGHKGAKKVTVQNLEVVKVDPDNNLLIIKGSIPGPRKSLLYIKNTVKGRN"
     gene            complement(3755..4063)
                     /gene="rpsJ"
                     /locus_tag="PK768_07255"
     CDS             complement(3755..4063)
                     /gene="rpsJ"
                     /locus_tag="PK768_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQA60805.1"
                     /translation="MAHQKIRIRLKAFDHAILDQSAKKIVETAKRTGAKVSGPIPLPT
                     DRSIITILRAPHKYKDSREQFESRTHKRLIDIKQPNQKTVDALMRLDLPAGVDIEIKL
                     "
BASE COUNT          862 a         1033 c          834 g         1381 t
ORIGIN      
        1 cacctttgta aatccaaacc ttaactccga ttctgccata tgtggtatgg gcttctgcaa
       61 atccgtaatc aatatcagcc cttaaagtct gcagcgggat ggtcccttca tggtacatct
      121 cggaccgtgc aatttctgca ccgccaagtc taccgctgac cattgtcttt atgcctttag
      181 ctcccgactt taacgccctg gatatggcct gcttcatggc ccttctgaaa gatatgcgcc
      241 tttccagctg tgatgcaatg ttttctgcca caagctgagc gtcaatatcg gggtttttga
      301 tttctactac atttatcgaa acctgcttgt cggtcatttg ctctagttct gcctttaatt
      361 catctacgcc gctgccgcct ttgccgatta ccattccggg ttttgcagtg ttgaccgtaa
      421 ctcttatgcg ttttgccgcc cgctcaatct caattctcga aatacctgcc tggtacagtt
      481 ttttcttaat gtactgacgg attttgtagt cttctaaaag ctggtctgca aagtccttct
      541 ccgaatacca ccttgagtcc caatctttaa ttatacctac tctcaaccca tgagggtgta
      601 tcttttgacc caaaacctat ccctcctttt cctttaaaac tatcgtaata tggctggtct
      661 tcttgtgtat tgaataaact ctaccctgag ctcttggtct ataccgcttc attattgggc
      721 cttggtcggc atagcactgg gaaatataca gggcgtccct gttcatgtcg aagttgtttt
      781 ctgcatttgc tatagctgaa cgcagcaatt tttccagtaa tggcgaggcc gcttttggcg
      841 taaacctcaa tatggttaag gcttcatcta catgttttcc cctgataagg tctaaaacga
      901 ctctaacttt gctcggtgaa atcctgacat atctagcccg tgccctcgct tccaacttgc
      961 atacctcctt tgctagctta ttaaatctaa ttatttcagt gcagtggacc tttcggtatg
     1021 tgaaccgtgg cctctaaaag tcctggtagg tgcaaattca cctaatttgt ggcccaccat
     1081 atcttctgtt atatatacag ggacatgctt tctgccatcg tggacagcta ttgtgtggcc
     1141 caccatttca ggaaatatag tggaagccct ggaccaggtc ttgataacct ttttttcccg
     1201 cttcttgttc atctcaacaa ttctagccag gagcttttta tcaacgaagg gtcctttctt
     1261 tgttgaacga ctcattaaat tacctccctt ctacttacgg cgcttgacaa tatatttatc
     1321 tgaagcttta tttttctttc tagtcttata tccaagggta ggcttgcccc aaggtgtaac
     1381 agggctcttg cggccaatag gagccttgcc ttcgccaccg ccgtggggat ggtcaacggg
     1441 attcattaca acaccgcgga cgtgtggtct cctgttcagc cagcgcgacc tgcctgcctt
     1501 accgattgtg atattctcat gatcaatgtt gccaacctga cctatagttg cccggcattc
     1561 ttccggtacc attcgcattt ctcccgatgg cagtctgagg gtcgccatgc cgccttccct
     1621 tgccatcagc tgagccgatg tacctgccgc cctaaccatc tggcctccct tgcccgggac
     1681 tagctcgata ttgtgaactg tggaacccac aggtatatct acgagtttaa gggcattgcc
     1741 cggcctgata tctgcattct caccggacat aacggtatca cctacgcgaa ggccgtccgg
     1801 ggctaaaata tagcgttttt ctccatctgc ataatgtagg agcgctatat atgccgtgcg
     1861 attgggatcg tattcaatgg ctgctacttt tgcaggtatg ccatctttat ccctcttaaa
     1921 atcgataatt ctatatttgc gcttgtggcc gccgccgcga tgtctcatgg tcattctgcc
     1981 ttgcatgttg cggccggctt tatttttcag tatttccacc aaggaccgct cgggcttttt
     2041 cttggttatt tcctcaaaag taggaacgct cataaacctg cggcccggag tggtgggctt
     2101 aaactttttt atagccacgt tgttccctcc ttctctatgt caaatcaaaa gccgtacttg
     2161 ctttaaacta tagtccttcg aagaattcga tggttttact gtcttcagtt aaagtgacaa
     2221 tggctttttt ccaatcgggc cttttccctt caaatcggcc catgcgcttc attttgcctc
     2281 tcatgttgat ggtgttgact tttgcaactt ttactccaaa aatcgcctct acagcattct
     2341 ttatttcggt tttgttggaa tttttatcaa ctacaaagac gtattttcta tcctgctgca
     2401 aatccatgct cttttccgta atccaggggc gaatgatgat gtcatgcgga tgtttcatcc
     2461 tgcatacacc tcctctaccc gtttagccgc ttcttgcgtc attatcaggt aatcgtgatt
     2521 taagatgtca tatacgttta aggtgttgac gtatgtggtc tttactccgg gtatgttacg
     2581 tgcagatttt acgacatttt catctttatc ggcaataact attaaagact ttttgtcaac
     2641 ctttaaattg cttaaaatgg aaaccatttg cttggtcttg ggcgcttcca tcgtcaagct
     2701 atccagcaga atgagttcat tctccttggt ttttgcactt agagctgatt ttaaggcaaa
     2761 tctcttcagc ttcttaggca ttttcatgct aaaggagcga ggcttgggtc cgaacactat
     2821 tccacctttt ctccatatgg gtgaccttat gctgccgtgc cttgctcttc cggtgccctt
     2881 ctggcgccag ggctttcttc cgccgccgga aacttcagcc cttgtcaagg tactggcggt
     2941 tcccagcctt tggtttgcca gatagcttag aacggcgcgg tgcattatat ctggccttat
     3001 ctcgacaccg aagacctcgt cggaaagttc tatttcccct actttatctc cttggatgtt
     3061 atataatgct actttcggca ttgggctcct cctttcactg ttagtttctg ccctttacgg
     3121 tgtttttgat atagagtaat gattttctgg gtcctggtat tgaacccttg attatcaaaa
     3181 ggttgttatc ggggtccact ttgactactt ccaggttttg taccgtgacc ttctttgctc
     3241 ctttatgccc cgcggctttt aagcccttaa cgactttccc gacaccggca gcacctatgg
     3301 aacctacgat tctgtgagat tttgagccgt gagacatagg gccccgtcta aagttccaac
     3361 gcttaatggt tcctgcaaag cccttaccct ttgatatgcc cgttacatca actttgtctc
     3421 cgggctggaa aatgtcgacc tttatttcgt caccaacgtt gtacttgtcg gcatccttga
     3481 ttttaaattc ctttaagtac cgcttaaatg gtacttggct ctttttgaat tggccttgca
     3541 ggggcttgtt taattttttt tctttgatat cttcatagcc aacttgtata gcactgtagc
     3601 cgtcattttc taaggtcctt ttgcgaacta caatgcatgg cccggcctgc accaaggtaa
     3661 caggtaaaga ttcacctgta tcgctaaaaa tctgagtcat accgattttt ttgcctaata
     3721 tggcctttga aatcatctaa gtcacctccc attgctacag ctttatctca atatctactc
     3781 ctgcgggcaa atccaatctc ataagagcgt caacggtttt ttggttgggt tgtttaatat
     3841 caattaatct cttatgggtc ctggattcaa actgttctcg ggaatccttg tacttgtgcg
     3901 gagcccgaag tatggttatg atactccggt ccgtgggtaa aggtatgggt cccgatactt
     3961 tggccccggt ccgctttgcc gtctctacaa tttttttggc cgattggtca agaattgcgt
     4021 ggtcgaaagc ctttaagcga attcttattt tttgatgggc catttgtgtc cctccttacg
     4081 ttcggaatta tgggttttta ttttattcaa
//
LOCUS       DAONUO010000187         4080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15487_length_4080_cov_4.683478, whole genome shotgun sequence.
ACCESSION   DAONUO010000187 DAONUO010000000
VERSION     DAONUO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15487_length_4080_cov_4.683478"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK768_07260"
     CDS             complement(<1..200)
                     /locus_tag="PK768_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQA60806.1"
                     /translation="MADRKDSFLKGALILTVAGFVVKALGALYRIPLALLIKDEGMGL
                     YQMAYPIYLILLSISTAGLPTA"
     gene            463..2040
                     /gene="pckA"
                     /locus_tag="PK768_07265"
     CDS             463..2040
                     /gene="pckA"
                     /locus_tag="PK768_07265"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HQA60807.1"
                     /translation="MDKLASIGLYNTKNIFKNLSVPRLVEEALKRGEGFLTDSGALNV
                     FTGKYTGRSPNDKFIVDEPSVHDKIWWGNNKPIEPENFENLLKRLMAYLQNRDLFIFD
                     GFAGADPKYRLPIRVINEYAYQNLFCRQLFIVPSDDELVNHEPGFTVICAPGFKADPE
                     TDKVNSEAFIILSFEKKLVIIGGSQYAGEMKKSIFSVLNYIMPQQGVLSMHCSANVGK
                     DGSTALFFGLSGTGKTTLSADPHRFLIGDDEHGWSDDGIFNFEGGCYAKCINLSREKE
                     PQIYDAIKFGAVLENVIVDEDTRIPDYDSDEVTENTRAAYPVEFIPGAVIPGVGGHPK
                     TVVFLTADAFGVLPPIAKLTREQAMYYFISGYTSKLAGTERGITEPQATFSSCFGAPF
                     LPLSPMVYAKLLGEKIEKHDVNVFLVNTGWTGGPYGVGKRMDLGYTRAMIKAALDGEL
                     DSVEYEEDPIFGLMIPKSCPEVPSQVLNPRNTWSDKDAYDKAAASLAESFAKNIAKFS
                     GIAPEVIAAGPKSAAGK"
     gene            complement(2122..2682)
                     /gene="spoVT"
                     /locus_tag="PK768_07270"
     CDS             complement(2122..2682)
                     /gene="spoVT"
                     /locus_tag="PK768_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HQA60808.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDREGEV
                     ILKKYSPIGELGDFAREYADSLHDSVNHITCIADRDAIIAVAGAPKKEYMDKSISSAL
                     EKIMDERRTVLIAKTGEKEYVPITTDEDEGKIKYSSQVICPIIAEGDPIGAVILLSKD
                     ADVSMGDMEVKVAETAAGFLARQMEQ"
     gene            complement(2774..3739)
                     /locus_tag="PK768_07275"
     CDS             complement(2774..3739)
                     /locus_tag="PK768_07275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQA60809.1"
                     /translation="MKRVIVLLLLALIVITASACQNIEKVEPEEGRVIDGQEVIAKVN
                     DEFILKSDYDREVANVKSVLEANGMDFSTAEGKKILREVKEKVLESMINDRLILWQAR
                     EHDISLDDEEFNEAIKQLEKYHGGKDALETYLKQQGYDRKTFEAQVREQLIINKFKEK
                     LTADVKVTEEEVKKYYDENKEMFELPSPEIRASHILVDTEEEAREILSKIRGGADFEE
                     LAKMYSKDPGTKDLGGDLGFFAKGKMVPEFEEAAFALNPGEVSDVVKTEYGYHVIKVT
                     GRRSSLSFEDAKEDIRNNLENAKKDKEFAKHIEEWEKQSKIEKYL"
     gene            complement(3784..>4080)
                     /locus_tag="PK768_07280"
     CDS             complement(3784..>4080)
                     /locus_tag="PK768_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60810.1"
                     /translation="TRNLIMISRIRALAAGLGIESIIQKGDIVNMKFHKSSKLSPELL
                     VAINLVLNRQVNIIGASTPTIAVKTRGNKGTKLLLRIQKILEEIRALQESGAVV"
BASE COUNT         1024 a         1045 c          825 g         1186 t
ORIGIN      
        1 attgccgtag gcagccccgc agtagaaatg gacagaagta tcaaataaat ggggtatgcc
       61 atctgataca ggcccattcc ctcgtctttt atcaagaggg ccagtggaat tctatacaat
      121 gctcccaagg cttttacaac aaatcctgca accgttaaga ttagtgcgcc ctttaaaaag
      181 gaatccttcc tgtctgccaa aaaaatatcc gcctttcttt tcaaattctt catctattat
      241 attctattcc ataggcagca gcaatattta ttgaaaagaa tctcactttg tttcgcatca
      301 cggtatactg ttctagtaag taaaatagcc gcaaaaaagc gcttatttaa agttcttgtt
      361 aaaaatttaa taaatttgag caaaataatt ctggtaagct cacaaaaaat gttatataat
      421 aggttttaaa ttgaattttt ttagaagcgg aggtagaaat caatggataa actggcttcc
      481 atcggtcttt acaacacaaa aaacatcttc aaaaatcttt cggttccccg ccttgttgaa
      541 gaggcattaa agagagggga aggttttttg accgatagcg gcgcactgaa tgtttttaca
      601 ggcaaatata cagggcgttc gcctaacgat aaattcattg tcgacgaacc ttctgtccac
      661 gacaaaatat ggtgggggaa caacaagccc atagagccag aaaactttga aaacctttta
      721 aagcggctta tggcatacct gcaaaacagg gaccttttca tatttgacgg ttttgcaggg
      781 gctgatccca agtaccgctt gcccattcgc gtcattaacg aatatgcgta ccagaatctg
      841 ttctgccgcc agctgtttat cgtcccatcg gatgacgaac tcgtaaatca cgagcccggc
      901 tttacggtca tatgtgctcc tggctttaaa gctgatcccg aaacggacaa agtcaactcc
      961 gaggccttta tcatcctcag ctttgaaaag aaactggtca tcatcggcgg cagccaatat
     1021 gccggcgaaa tgaaaaaatc catattttcg gtactaaact atataatgcc ccagcaggga
     1081 gtcctttcca tgcactgctc agcaaacgtg ggcaaggacg gttctacggc cctgttcttc
     1141 ggcctttccg gcaccggcaa aaccaccctt tccgccgacc cgcatagatt cctcatcggc
     1201 gacgatgaac acggctggtc cgatgacggc atctttaatt ttgagggcgg ctgctacgcc
     1261 aagtgtatca accttagcag ggagaaagag ccccagattt atgacgctat aaagttcggt
     1321 gcggtattgg aaaatgtcat cgtagatgaa gatacgagaa taccggatta cgacagcgat
     1381 gaagttacgg aaaatacccg tgccgcttac ccggtagagt ttattcctgg cgctgtcatc
     1441 cccggtgtgg gcggtcatcc caagaccgtg gtattcctta ccgcagacgc attcggcgtg
     1501 ctgcccccca tagccaagct cactagggaa caggctatgt actactttat ttccggctat
     1561 accagcaaac tggccggcac agaaagggga attaccgaac cccaggccac attttcttcc
     1621 tgtttcggag ctcccttcct gcccctttca ccgatggtat acgccaaact cttgggcgaa
     1681 aaaatcgaaa aacacgatgt caacgtattc ctggttaaca ccggatggac aggtggcccc
     1741 tacggtgttg gaaagcgcat ggacctcgga tacaccaggg ccatgatcaa ggcagccctg
     1801 gacggtgagc tggactcggt agaatatgaa gaagatccca tcttcggctt aatgattcca
     1861 aaatcctgcc ccgaggttcc ctcgcaagtg ttaaacccca ggaacacctg gagcgataag
     1921 gatgcctatg acaaggcggc cgcaagtctt gcagaaagct tcgccaagaa tattgccaag
     1981 ttttccggca tcgctcccga agttatagca gccggaccga aatcagcagc gggcaaataa
     2041 ctgctatccg acgacccgat tgacgcgggg ctttgccccg tctcctctca ataaaaaaat
     2101 agcagctagg ctgctatttt tttattgctc catttgcctt gccagaaatc ctgccgcagt
     2161 ttcagctacc tttacttcca tatctcccat acttacatcc gcatccttgg agagaagtat
     2221 tacggcccct attgggtcgc cttctgcaat aatcggacat attacctggc ttgaatactt
     2281 gattttgcct tcatcttcat ccgtagtaat aggtacgtat tccttttcac cggtcttggc
     2341 tatgaggacg gttctgcgtt catccatgat cttctcaaga gcggaactta tggacttgtc
     2401 catgtattcc tttttaggtg ctccggcaac ggctataatt gcgtctctgt cagcaataca
     2461 agttatatga tttacggaat catgaagtga atctgcatat tccctggcaa agtcccccag
     2521 ctcccctatc ggagaatatt ttttcagtat tacttcccct tcccgatcag taaatatctc
     2581 aagagggtcg ccttcgcgaa ttctgagggt ccgacgaatc tccttgggta tcactacacg
     2641 tcctagatca tctatgcgtc gaacaattcc tgttgccttc aattaatttc cctccttata
     2701 atctggatta tccttcagat atagtatata acattgatgg aggttctatt cattgaaggc
     2761 aggatgagat attctataaa tatttttcaa ttttcgattg tttttcccac tcttctatgt
     2821 gtttagcaaa ctccttgtct ttttttgcgt tttccaggtt atttctgata tcctccttgg
     2881 catcctcaaa gctcagggaa ctgcgcctgc cggtaacttt gatgacatga tatccgtatt
     2941 cggtcttaac cacatcactt acttcaccgg gatttaaggc aaaagccgct tcttcaaatt
     3001 ccggaaccat cttgcccttg gcaaagaatc ctaagtcccc gcccaaatct ttggtgccgg
     3061 ggtccttgga atacatctta gccagctcct caaagtcggc tccacccctt attttgctca
     3121 aaatctccct tgcttcctcc tccgtatcca ccaagatatg gcttgctcgg atttccggcg
     3181 agggaagctc gaacatctcc ttgttttcat cgtagtattt cttaacttct tcttcggtaa
     3241 cttttacatc cgctgtgagt ttttctttaa acttatttat tatgagctgc tccctgacct
     3301 gggcttcgaa agtctttcta tcgtaaccct gctgctttaa ataggtttcc agagcatcct
     3361 ttccaccgtg gtacttttcc agttgcttaa tagcctcatt gaattcttcg tcatccaggc
     3421 tgatgtcgtg ttctctcgcc tgccacagta tcaaccggtc atttatcatg gattccaata
     3481 ccttttcttt gacttccctt agtatcttct tgccttctgc cgttgaaaag tccatcccgt
     3541 tggcctctag gacactttta acattggcaa cttcgcggtc gtagtcagat ttcaggatga
     3601 attcatcatt gaccttggct atgacctcct gcccgtcaat taccctgcct tcctcaggct
     3661 ccaccttttc tatgttttga cacgcggaag ctgtgattac aattagtgcc aaaagcaaca
     3721 atacaattac cctcttcata ttatcctcct catcccggta gaaagcttgc cctgtacaaa
     3781 atattatact acagcgccgg actcctgcaa agctcttatt tcttccaaaa tcttctgtat
     3841 gcgaaggagc agcttcgtac ctttgttgcc ccttgtcttt accgcgattg tcggggtgct
     3901 tgcacctata atattgacct gcctgttaag caccaggttg attgccacga gcagctccgg
     3961 gctcaatttg ctgcttttat ggaacttcat atttacaata tcgccttttt gtatgatgct
     4021 ttcgatgccg agccccgcag ccaaagccct gatgcgggat atcattatga gattcctggt
//
LOCUS       DAONUO010000188         4057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15606_length_4057_cov_4.009495, whole genome shotgun sequence.
ACCESSION   DAONUO010000188 DAONUO010000000
VERSION     DAONUO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15606_length_4057_cov_4.009495"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PK768_07285"
     CDS             <1..251
                     /locus_tag="PK768_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQA60811.1"
                     /translation="AKEKHGMRYTQYRGLRKVQHHLTLLFACMNLKKLAMWKRRQQKM
                     PPAPHPVAAAATFFVSNLLFAIKNSFQSFSWKLFLSTV"
     gene            344..1117
                     /locus_tag="PK768_07290"
     CDS             344..1117
                     /locus_tag="PK768_07290"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQA60812.1"
                     /translation="MALEDDIQQTFTAVEIELPETIKLLLELKDKFNIKEFNADTILK
                     SVRDEDAQKRIFLYNRIRELLNTCRSCPLCLSDWHTQKVPGEGQLNSPLVLIGEGPGL
                     DEDKLGRPFVGRAGQLLTTILEKLNINREKIYITNVIKCRPPQNRTPTQKEIKACSHN
                     LELELSIISPKVIIALGAVPLNYFKPGSSIMRERGNWIVHGDFWIMPTFHPAYVLRQH
                     GKTLTKVKWAVWQDFNNAIKKLTELSPGTLSKISLDTKE"
     gene            1122..2444
                     /gene="miaB"
                     /locus_tag="PK768_07295"
     CDS             1122..2444
                     /gene="miaB"
                     /locus_tag="PK768_07295"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQA60813.1"
                     /translation="MKYKILTWGCQMNIHDSEAIAGILQKMDYCPADTLKEADVIILN
                     TCCVRENAERKVYGRIAQLKQFKQRKPNLILAVTGCMVQQPHVVEHISEKMPYVDILF
                     GIKNVHKLPELIENSRLAELPVVEVMGGSEIEENLPIERMDNSRAWVTITYGCNNYCS
                     YCIVPYVRGREKSRRPEDIIREVEGLAKEGFIEINLLGQNVNSYGKDLEKPVAFHELL
                     RRVNDIDGIKRIRFLTSHPKDLSDELILAMKECEKVCEHIHLPVQAGSTRVLSMMNRK
                     YSREHYLNLIEKLREAIPDIAISTDIIVGFPGETEEDFLDTLDLVKKVQFDSAYTFMY
                     SKRKGTPAAEMPNQVDDEVKKDRLNRLMQVQDAITAKKNMMLKGTVQEVLVEGISKGN
                     KNRLSGRTRTNRLVNFEGTEDLIGKLVNVKIIEPHTWSLLGEIVAKNA"
     gene            2460..>4057
                     /gene="mutS"
                     /locus_tag="PK768_07300"
     CDS             2460..>4057
                     /gene="mutS"
                     /locus_tag="PK768_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQA60814.1"
                     /translation="MDTPMMKQYKEIKSKYRDYIVFFRLGDFYEMFYEDAHICSRELE
                     IALTSRDPQNRVPMAGVPYHAADQYIAKLVSKGYKVVICEQVEDPKLAKGIVKREVVK
                     IITPGTITDLNALEDNKNNYLACVYKHKGRFGLSFVDLMTGEFNVTELTSSYPYHEII
                     NEISRFEAKECLVSQNLIEDKPLNRNLNKNLKIYFTVKDEDYFHEDETISLLISHFGK
                     EKIVKLKDKRFALRAAGACLKYLNETQKLNLSHINSITYYKTCDFMILDYSCRKNLEI
                     TESLYDGKKMGSLLWVLDKTSTAMGGRLIRKWLERPLIDILRIKERQDAIEELLNDFF
                     LRADIKEQLKNVYDLERLTGKLVCGNANARDLLAIKTTIIRLPRLKQVISRCSSKLLH
                     QIYEQLDTLEDIYSLLDDSIAENPPLTIKEGNIIKDGYNSEIDLLRKASREGKAWIAD
                     LELKERERTGIKSLKVGFNKVFGYYIEVTKSNLAMVPDDYIRKQTLANSERYVTEQLK
                     EYEELILNANEKLHELEYDMFCKI"
BASE COUNT         1442 a          688 c          832 g         1095 t
ORIGIN      
        1 acgctaaaga aaaacatggt atgcgttata cacaatatcg aggcttgagg aaagttcaac
       61 atcacctcac gcttcttttc gcatgcatga atttaaagaa gttggcgatg tggaaaagga
      121 ggcaacagaa aatgccacct gcaccacatc cagtagcggc tgctgctacc ttttttgtgt
      181 caaatttatt gtttgccata aaaaacagct tccaaagttt ttcctggaag ctgtttttgt
      241 ctacagtctg aaaagacctt ggatcttttt ttctttattt tgcgtttgtt taatgtttaa
      301 ataaatttat gctataataa ggcatagaat aggaggttat cctatggcct tagaagacga
      361 tattcaacaa acctttacag cagtagaaat tgagctgcca gaaactataa aattactgct
      421 tgaactaaaa gacaaattta acataaaaga gtttaatgct gatacgatac ttaaatctgt
      481 cagggatgaa gatgcacaaa aaaggatttt tttgtacaac aggatacgag aactactaaa
      541 tacctgccgg agttgtcccc tttgcctgtc ggactggcat actcaaaagg tgccaggtga
      601 agggcaatta aattctccgc tggtcttaat cggtgaaggt cccggtcttg atgaagataa
      661 actgggccgt cctttcgtag gaagagccgg ccagctacta acaactattt tggaaaagct
      721 taatataaat cgtgaaaaaa tatatatcac caacgtgatt aaatgccgcc cgccacaaaa
      781 taggacacca acacaaaaag aaatcaaagc atgctctcat aaccttgaac tagagttatc
      841 aataatttca ccaaaagtta ttattgcttt aggcgctgtt cccttaaact actttaaacc
      901 cggcagcagc ataatgcgag agaggggcaa ttggatagtt cacggagatt tctggattat
      961 gcccactttt caccctgcat atgtcctgcg ccaacacgga aaaaccttaa caaaagttaa
     1021 atgggcggta tggcaagatt ttaacaatgc aataaaaaaa ttgacagagt tatctccagg
     1081 aactttatcc aagataagtc ttgacacaaa ggagtgaaaa cgtgaaatat aagattctaa
     1141 cctggggctg tcagatgaac attcacgaca gcgaagcaat agcaggtatc ttgcaaaaaa
     1201 tggattattg ccctgctgat acattgaaag aagcagatgt gattatttta aacacctgtt
     1261 gcgtcagaga aaacgcagag cgaaaagtgt acggtagaat agcacagtta aagcaattta
     1321 aacagagaaa gccaaatttg atactagcgg ttaccggatg catggtccaa cagcctcatg
     1381 ttgtggaaca tatatcagaa aaaatgcctt atgtcgatat tttgttcggc ataaaaaatg
     1441 tccacaagct gcctgaactt attgaaaact ccaggctagc tgaacttccg gtagttgaag
     1501 taatgggggg ctccgagata gaagaaaacc ttcccataga gcgaatggac aattcacgtg
     1561 catgggtaac tattacttac ggctgtaata actattgcag ttactgcatt gtaccctatg
     1621 taaggggaag ggaaaaaagc cgcaggccgg aagatattat cagagaagtt gaaggccttg
     1681 ccaaagaagg ttttatcgag attaaccttt taggtcagaa tgtaaactcc tatggaaaag
     1741 atctagaaaa acctgttgca tttcacgaac tgttgagaag ggtaaacgat atagatggca
     1801 tcaaacgtat aagattttta acttcacatc caaaggattt gtccgatgag cttatattgg
     1861 caatgaaaga atgtgaaaag gtctgtgaac acatccactt accagttcaa gcaggaagta
     1921 ctcgagtttt atcaatgatg aacaggaagt acagcaggga acattatttg aatttaattg
     1981 aaaaactccg ggaggcaatt cctgatattg caatttccac tgatattatt gttggttttc
     2041 ccggtgaaac cgaagaggat tttttagaca ctcttgattt ggtaaaaaag gtgcagtttg
     2101 attcagcata tacatttatg tactcaaagc gcaaaggtac tcctgccgct gaaatgccaa
     2161 atcaagtgga tgacgaagta aaaaaagacc gattaaacag attgatgcaa gtccaagatg
     2221 ccataacagc taagaaaaat atgatgctta aaggtaccgt tcaagaggta ttagttgagg
     2281 gaattagcaa aggcaacaaa aatagattgt caggtcgcac cagaacaaat agactagtta
     2341 atttcgaagg cactgaagat ttaattggta aactggtaaa tgtaaagatt attgaaccgc
     2401 acacctggtc attattagga gagattgtag caaaaaatgc gtagggggtt tttctttcga
     2461 tggatactcc tatgatgaag caatataaag aaataaaaag taaatacaga gattatatag
     2521 ttttttttcg actgggcgat ttctatgaaa tgttctatga agatgctcat atttgttcaa
     2581 gagaacttga aatcgcattg acatcaagag atccccaaaa tagagtgcct atggccgggg
     2641 tgccttacca tgcagcagat caatatatcg ctaaacttgt atcaaagggc tacaaagtgg
     2701 ttatttgcga acaggtggaa gacccaaagc ttgctaaagg cattgttaag cgagaagtag
     2761 ttaagattat cacacctggg accattactg atttaaacgc tcttgaagat aacaaaaaca
     2821 actatttagc atgtgtttat aaacacaaag gacgatttgg ccttagcttt gtggatttga
     2881 tgaccggtga attcaatgtt acggaactga cctcatctta cccctaccat gaaatcataa
     2941 atgaaataag tcgctttgaa gcaaaggaat gcttggtaag tcagaattta attgaagaca
     3001 agcccttaaa tagaaacctc aacaaaaatt tgaagattta tttcacagta aaggatgaag
     3061 actactttca tgaagatgaa actataagcc tactaatatc gcactttgga aaagagaaaa
     3121 tagtaaagct taaagacaaa agatttgccc taagggcagc gggtgcgtgt ttaaaatact
     3181 taaatgaaac acaaaaatta aacctatcgc atatcaattc tattacttac tacaaaactt
     3241 gcgatttcat gattttagat tatagctgcc gcaaaaactt agaaattacg gaaagcctgt
     3301 atgatggcaa aaaaatggga agcctcttat gggtattaga caaaacttct acagccatgg
     3361 gaggtagact aattaggaaa tggctggagc ggcctcttat tgatattttg aggattaagg
     3421 aacggcagga tgcgatagaa gaattattaa atgatttctt tttaagagca gatattaagg
     3481 aacagttaaa aaatgtctat gatttagaac gtttgaccgg aaagttagtt tgtggaaatg
     3541 ccaacgcaag agatttactg gcaatcaaga ctacgataat tagattgcca agattaaaac
     3601 aggtcatttc caggtgcagc tctaaacttt tacaccaaat ttacgagcaa ttagatacgt
     3661 tagaagatat ttattcatta ttggatgatt ccattgcgga aaaccctccc ctgacaatca
     3721 aagaaggaaa tattataaaa gatggctata attcagaaat agatttattg agaaaagcat
     3781 cccgtgaagg caaagcttgg attgctgatt tagaattaaa agagcgggaa agaaccggaa
     3841 ttaaatcgct taaagttggt ttcaataagg tcttcggcta ttacattgaa gtaaccaaat
     3901 caaatcttgc catggtcccc gatgactata taagaaaaca aacactagct aattcggaac
     3961 gctatgtaac cgagcaattg aaggaatatg aagaattgat actcaatgca aatgaaaaac
     4021 tacatgaatt agaatacgat atgttttgta aaataag
//
LOCUS       DAONUO010000189         4025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15756_length_4025_cov_4.053401, whole genome shotgun sequence.
ACCESSION   DAONUO010000189 DAONUO010000000
VERSION     DAONUO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15756_length_4025_cov_4.053401"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PK768_07305"
     CDS             <1..902
                     /locus_tag="PK768_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60815.1"
                     /translation="TLGLVGETGAGKTTTVKTIMRILPVPPSVIKSGEIFFKGEDLLK
                     KTEKEMRSIRGAKISMIFQDPMTSLNPVLTVGEQIMEAIGLHNRVSKRKAIELAEEML
                     ETVGIKGDRLSDYPHQFSGGMKQRVVIAMALACNPQLLIADEPTTALDVTIQAQVLEL
                     MQRLKRQYKTSLIMITHDLGIVAEICDYVAIMYAGQIVEYGSLEDIFLHAQHPYTKGL
                     FSCIPDLYSDDKELIPIPGLMPDPSNLPSGCRFHTRCQYSFDRCEIQEPPSVEVSPGH
                     HVKCFFAVPEKGEPQGTPLGRPV"
     gene            886..1881
                     /locus_tag="PK768_07310"
     CDS             886..1881
                     /locus_tag="PK768_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA60816.1"
                     /translation="MEGRYDKEGGEDLKMALLEVKNLKKYFKTKSGLLHAVDGVDLHI
                     NKGETLGLVGESGCGKSTIGRLVLRLIEPTEGEIIFEGKDLGKLNKSEMRQMRRKMQI
                     VFQDPYASLDPRKNVFELIAEPLIVQRIYSNKRELERRIYELMEIVGLAQRLVNSYPH
                     ELDGGRRQRVGVARAIALRPVFMVLDEPVSALDVSIQAQIINLLLKLQREFGLTYLFI
                     SHDLSVVKHISDRVAVMYLGQIVELADCKEIFKEPLHPYSQALLSAVPVPRIDLKREK
                     IILEGEVPSPINPKPGCRFTSRCRYCQDICKTETPVLREIYPGRFVACHFPQKGQ"
     assembly_gap    1964..1973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2184..2372)
                     /locus_tag="PK768_07315"
     CDS             complement(2184..2372)
                     /locus_tag="PK768_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60817.1"
                     /translation="MAFKTNHEDIKQFVLKPTDIGYDAEAGAELSPNIRGAYGQTPRQ
                     RASSTTSTRPPIMLHPGE"
     gene            2607..3989
                     /locus_tag="PK768_07320"
     CDS             2607..3989
                     /locus_tag="PK768_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA60818.1"
                     /translation="MSKKYDVIIVGAGPAGIFTALELVKEATELSILIVEKGKKLESR
                     QCPAKEKNISCIRCNPCSIVNGWGGAGAFSDGKLTLTTEFGGWLDEYISRSQVMELIE
                     YVDSIYLNYGAPEEIHGVDEKRIRELQQKAATADLRLIPARIKHLGTDKCWDILKEMR
                     DFLDSKVDIKTSTEVKQLLVENGQVAGIETTRGEIFLGRFVVAVPGREGAQWFSKEAQ
                     RLKLDMAINPVDIGVRVEVPAVVMEHLTDVVYESKLIYFSKSFDDKVRTFCMNPYGEV
                     VTENNSGLITVNGHSYSGRRTSNTNFALLVSKTFTEPFKEPISYGRNIATLANMLGGG
                     VLVQRLGDLLSGRRSTPERIKRGLVEPTLKEATPGDLSLVFPYRHMVSLLEMLEAMDK
                     IAPGVYSNHTLLYGVEVKFYSSRPALTSNLETQVQNLFAAGDGAGVTRGLAQATASGI
                     IVAREILKRM"
BASE COUNT         1111 a          900 c         1030 g          974 t
ORIGIN      
        1 agaccctggg gctggtaggc gagacaggtg caggtaaaac aactaccgta aagaccatca
       61 tgcgtatctt gcccgtccct ccttcggtga taaagagcgg agagatcttt ttcaagggcg
      121 aggatctgct gaaaaaaacg gaaaaggaaa tgcgctctat ccgaggagca aaaatatcca
      181 tgatatttca ggatcccatg acctccttaa atccggttct gactgtcggg gagcagatta
      241 tggaggctat tggccttcat aacagggtaa gcaagcgtaa agccattgaa ctggcggagg
      301 aaatgctgga gacggtaggc ataaaggggg accgcctcag cgactatccc catcagtttt
      361 ccggaggcat gaaacaaagg gtggtaatag caatggccct ggcctgcaat cctcagcttt
      421 tgatagcaga tgagccaacc accgcccttg atgtcaccat acaagcccag gtgctggaat
      481 tgatgcagcg gctcaaaagg cagtacaaaa catccctgat catgataacc cacgatttgg
      541 gcatcgtcgc cgaaatatgt gactatgtgg ccataatgta cgcaggccag attgttgagt
      601 acggttccct ggaagacata tttcttcacg cccagcatcc gtacactaaa ggcttgtttt
      661 cctgcatacc cgatttatac tccgatgaca aagagcttat acctataccg ggcctcatgc
      721 cggatccatc caacctacct tcgggctgca gatttcacac gagatgccag tacagcttcg
      781 acaggtgcga aatacaagag cccccatcgg ttgaggtatc accgggacat catgtgaagt
      841 gcttctttgc tgtaccggaa aaaggggaac cacaggggac gccccttgga aggccggtat
      901 gacaaagaag gaggtgaaga tttaaagatg gcacttttag aggtaaagaa cttaaaaaag
      961 tactttaaga cgaaaagtgg actgttgcac gcagtagatg gagttgatct tcacataaat
     1021 aaaggggaaa ccctgggctt ggtgggtgag tccggctgcg gcaagtccac cataggtaga
     1081 cttgtccttc gcctgataga gccaacagaa ggtgaaatca tatttgaagg caaggattta
     1141 ggtaaactca ataaaagtga aatgcgtcag atgcgccgca aaatgcagat agtctttcag
     1201 gatccatatg cttccttgga cccgcgcaag aacgtgttcg aactaattgc agagcccttg
     1261 atagtacaga gaatatattc aaataaaagg gagttggaaa ggcgcattta tgagctgatg
     1321 gaaatcgtcg gtctagccca gcgccttgtg aattcttatc ctcatgaact tgacggtggc
     1381 aggaggcaga gggtgggagt tgcccgggct atagctttaa gacctgtctt tatggtgctg
     1441 gatgaaccgg tatcggctct tgacgtatcc atccaggcac agattataaa tcttttactt
     1501 aaactccaga gagaattcgg ccttacatat ctgtttattt cccatgactt aagcgtggta
     1561 aagcacatca gcgaccgggt ggcggtcatg taccttggcc agatagtcga gctggcggac
     1621 tgcaaggaaa tctttaaaga gcctctacat ccttacagtc aggccctgct atcggcagtg
     1681 cccgtccccc gcatagacct aaagagggaa aaaataattc tcgagggaga ggtgccaagt
     1741 ccgattaatc caaaacccgg atgccgcttt accagccgat gcaggtactg ccaggatatc
     1801 tgtaaaaccg aaacgccggt tttgagggaa atctatccgg gcagattcgt agcatgccat
     1861 tttccccaga aggggcaata aatatatcgc tgtggccgcc agtaaaaatt catccctttc
     1921 aaaggattgt tggaactaat atcatcggta gagatgacga cagnnnnnnn nnnttggtac
     1981 tgcacttcgc agaggttcac gggcaattcg gcatcctgcc gagtgcccgg cagcatgcag
     2041 gatacactct gcctctgcta cgcttacgcc aaacttaaga ccaactacgg tcttgcttga
     2101 atggtgcgct tgtcgtcatc tttaaatttt ttagagcaac tttgaagggg atgtagattt
     2161 aattcttggc ggccacgaac attttactcc ccggggtgta gcatgatggg cggtcgagtg
     2221 ctggtggtac tgctagctct ttgccgtgga gtttgcccgt aagcccctct tatattaggc
     2281 gaaagttccg ctcccgcctc agcgtcatat ccaatatctg tcggctttag gacgaactgt
     2341 ttaatatctt cgtggttggt tttaaatgcc atgacaatcc tccttgtatt ttaattccct
     2401 cctctgtagt attgcaataa gccaatacat gtattcataa aattttttcc gaacaatgaa
     2461 ccaaaatcaa atagaatatt ccgaaaacaa aattgatttt ccgccccagt tttgttatat
     2521 ttatattgtt gcaaattaat ttaacatggt ataatcggca atgctgtgtc aacagcgcca
     2581 ttttggcaat gaaaatggag gttgttatgt ctaaaaagta tgatgttatt atagttggtg
     2641 caggtcctgc aggaattttt acggcccttg agctggtaaa ggaggctaca gagctttcga
     2701 tacttattgt agaaaaggga aagaaactgg aaagccggca gtgtcctgca aaggaaaaaa
     2761 acatttcctg tatccgctgc aatccctgtt ccatagtcaa cggttggggc ggggcagggg
     2821 ccttcagtga cggcaagctc acccttacca cagaatttgg aggatggctt gatgaatata
     2881 taagccgcag ccaggtgatg gaacttatag aatacgttga cagcatatat ttgaattacg
     2941 gcgctccgga agaaatccac ggggtagatg aaaaacggat cagggagctg cagcaaaaag
     3001 cagccaccgc agatctgagg ctcataccgg caaggataaa gcacctgggc acggacaagt
     3061 gctgggatat tttaaaggaa atgcgagatt tcctggattc taaggtggat ataaagacat
     3121 ccaccgaagt gaaacagctt ttagtagaaa acgggcaggt tgccggcatc gaaacgacga
     3181 gaggcgaaat tttccttggc cgctttgtgg ttgcggtccc tggtcgagaa ggggcccagt
     3241 ggttttcgaa agaagcccag cggcttaagc tcgatatggc cattaatcca gtggatatcg
     3301 gcgtgagggt ggaagtcccc gcagttgtaa tggaacattt aacagatgtg gtttacgaat
     3361 cgaagctgat atatttctcc aagtcctttg acgataaagt caggaccttt tgcatgaatc
     3421 cctacggtga ggtggtcacg gaaaacaaca gcggcctgat taccgtcaac ggccacagct
     3481 attcgggtag gaggacttcc aacaccaact ttgccttact tgtcagcaag accttcacgg
     3541 agcccttcaa agaacccatt tcttacggca gaaatatagc aaccctcgcc aatatgcttg
     3601 gtggtggagt gctggtgcag cgcctggggg accttctgtc tggcagaagg tctacgccag
     3661 agaggataaa gcggggcttg gtcgagccca ctttaaaaga ggctacaccg ggggatttga
     3721 gcttggtatt cccctaccgg catatggtaa gcctcctgga aatgctggag gccatggaca
     3781 agatagcacc gggagtgtat tcaaaccaca ccctgctcta cggtgtcgaa gtaaagtttt
     3841 attcttccag gcctgccttg acttcaaacc tggaaaccca ggttcagaac ctgttcgcag
     3901 ccggtgacgg agcaggtgtg acaaggggac tggcccaggc aaccgcgtct ggaataatag
     3961 ttgccaggga aatattaaag cgcatgtaac aaaaaaatta aagcctagtc ccgttatagg
     4021 tttta
//
LOCUS       DAONUO010000190         4015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15795_length_4015_cov_3.518182, whole genome shotgun sequence.
ACCESSION   DAONUO010000190 DAONUO010000000
VERSION     DAONUO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15795_length_4015_cov_3.518182"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PK768_07325"
     CDS             complement(<1..1031)
                     /locus_tag="PK768_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HQA60819.1"
                     /translation="MSKMKRSGVLLVTCILILAMALGVGCTQKPAEKTPGEESQGGKA
                     QIEIPKRVMAIGSASSGSLFVTYTAAWADMIMKHVDGLIITVEPGGSSQNMQTIHNGD
                     NEFGITSTFQTYPGYHGIGWADGTKYQNVNSFIPAYSYEGVFFTTANRNDINSIQDLN
                     GKIVSFGYAGGGSDFTGRDIVEFFGIKPKEIVNASWSDVGGMIADGLVDAVFYLAGHP
                     ASFIQELELSHDLKFFGLTDDDFEKWLEKYPYYNVGTLASGTYKNMTEDYKALRGWNF
                     LAISPDLPEDFVYELTKLTWENVEAIHNAHISFKQTDLENVKYMNLPLHPGAKRYYEE
                     KGVELPHCLL"
     gene            complement(1263..2468)
                     /locus_tag="PK768_07330"
     CDS             complement(1263..2468)
                     /locus_tag="PK768_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQA60820.1"
                     /translation="MYYAVDWVDRVDFPKMREMRVQRLRETMVKHNLDALITFRAENI
                     RYMTNMRPLWWPISFLSRNASMMTQTSSPTLYVTSGDAPRCRATMTWMDPDDIKPCGT
                     MEEPGIVDTMIKQFVQTLKEKGFTKGRIGVDAGTFVMLEKLKAALPDCEVVDGDACVI
                     EAKAILNPEEIKLMKLSSKIAEIGMQTAIDHIRTGARNCEILGEVMKVFYAFGMEVPQ
                     CNLIVTSGEETAPLHRFASDRTIRHGDLVFMDLGGCFNGYFSDFTRTVVHGKPNDMQK
                     RIYNAVYEEMMVVHEMLKPGVTNEELNIAARNVIKKHGFEEWGFHGVLGHSINVSGLC
                     APLIGEIAAAGEEVFEFQPGMCFSCEPAILVPNVPGGGGVRLEDTILVTETGNEILTK
                     YPYCDELLG"
     gene            complement(2655..>4015)
                     /locus_tag="PK768_07335"
     CDS             complement(2655..>4015)
                     /locus_tag="PK768_07335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQA60821.1"
                     /translation="IMLLDQDGKISFYNRKAEQLLHIKDNYSSHIKSILDKSKSYGKD
                     FFVTIDDKSYHIEIDNNSFGDDLGTIIVIEDIKNIEKIEKSYRHSLHERGLTAKYTFN
                     NIIYKSKKMEQLIMKARQFAKSDSTILIEGESGTGKELIAQAIHNASGRSEEAFVAVN
                     FAALSENLCESELFGYEEGAFTGAKKGGKKGLFALAHKGTIFLDEIGDATINIQKKVL
                     RAIQERQILPVGGSQVIPVDIRIIAATNRDLQAMVNEGLFRQDLYYRLSVLPLRLPSL
                     RERPEDIFPLFINILKNQFGINLEKFPKALKEELERYKWPGNVRELRNVAEYMANFIQ
                     LNFDWEEELLSIIRNKINKTMDANDAHAEAIIRRLEEQSQILNFISILKTLDSPPFKW
                     TRKRLCEALENSGVSMSESQVKRHLLVLKELDLIQATTGSGTHIKESGRAVINQFKRN
                     MT"
BASE COUNT         1000 a          965 c          755 g         1295 t
ORIGIN      
        1 aggaggcagt gcggaagctc gacacctttt tcctcatagt acctcttagc gcccggatgt
       61 agcggtagat tcatatactt aacgttttct aaatccgtct gtttaaagct gatatgtgca
      121 ttatgaatag cttctacatt ttcccatgtt agtttagtaa gctcgtagac gaagtcttcc
      181 ggaaggtcag gagaaatagc aaggaagttc catccacgaa gagctttata atcttctgtc
      241 atattcttgt aagttccact agcaagggta cccacgttat aataggggta cttctctaac
      301 catttttcaa agtcatcatc ggttaatcca aagaacttga ggtcatggga taactccagt
      361 tcctggataa acgaagctgg atgacctgct aaatagaata cggcatcaac caatccgtct
      421 gctatcatac ccccgacatc agaccaactc gcgtttacta tttcctttgg ctttatgcca
      481 aaaaactcta ctatatctct tccggtaaag tcactgccgc cgcctgcata cccaaaggat
      541 acgatcttac cgttcaaatc ctgtattgaa ttaatatcat tacggtttgc cgtagtaaag
      601 aacacaccct cataggaata tgcgggaata aatgaattta cattttgata ttttgtacca
      661 tctgcccatc caatcccatg gtagcccgga tatgtctgaa atgttgaggt tataccaaat
      721 tcattatccc cgttgtgaat agtctgcata ttctgggatg acccgccggg ttccacagtg
      781 ataatcaggc cgtctacgtg cttcattatc atatctgccc aagccgcagt ataggttaca
      841 aacaagctgc ctgaggaagc acttcctata gccattactc ttttaggaat ctcgatctgg
      901 gccttacctc cctggctttc ttctcccggt gtcttttctg ccggcttttg cgtgcaacct
      961 actcccaaag ccatggccaa aatcaggata caggttacca atagaactcc gcttcttttc
     1021 atcttgctca tttaataatt cccccttcca atttttagga ctgttatcgt gccaattaca
     1081 tacctgccat ttctatggag atactatgcc tgttttacat taccgtttgt ttcagcctaa
     1141 tggatggata tcttgccgga attctatttt gtataatcac tactaatctg caagtcttgt
     1201 tacctggttc cgttcaagtg cttatttagg aggaagtgaa ttacacttcc tcctaaataa
     1261 gattatccaa gcagttcatc acaataggga tattttgtca atatttcatt gcccgtttca
     1321 gttacgagaa tggtgtcctc caaccgcact ccgcctccgc ctggaacatt gggtaccaat
     1381 atagccggtt cacaggagaa acacattcca ggctggaatt cgaatacctc ttcaccggcg
     1441 gccgctattt ctcctataag tggcgcgcaa aggcccgaaa cgtttatgga gtgtcctaat
     1501 acaccatgga agccccactc ttcaaagccg tgcttcttga taacatttcg agcggcaata
     1561 ttcaattctt cgttggtaac ccccggttta agcatttcat gcacaaccat catttcttcg
     1621 taaaccgcat tataaatacg cttttgcata tcgttgggct tgccgtgtac caccgtcctc
     1681 gtaaagtccg agaagtaccc gttgaagcag ccgccaaggt ccatgaatac caagtcgccg
     1741 tgtcttattg ttctgtctga agcgaaacga tgaagtggag cagtctcttc tcccgaggtt
     1801 actataaggt tgcactgggg cacttccata ccgaacgcat agaatacttt cataacttct
     1861 cccagtattt cgcagtttct tgcaccggtc cttatatggt ctattgccgt ctgcattcct
     1921 atttcggcta ttttcgaaga tagtttcata agttttatct cttcggggtt cagaatcgct
     1981 ttagcttcta taacacatgc atcaccgtca acaacctcgc aatccggaag ggctgctttc
     2041 agtttctcga gcattacaaa tgtacctgcg tctacaccta ttcttccctt tgtaaagcct
     2101 ttttctttca gagtttgtac gaactgtttt atcatggtat caacaatacc gggttcttcc
     2161 attgttccgc agggcttaat atcatccgga tccatccatg tcattgtagc acggcaacgt
     2221 ggtgcatctc cactggttac gtaaagagtc gggctgctgg tctgtgtcat cattgaagca
     2281 tttctggata ggaaagatat cggccaccac aagggcctca tgttggtcat gtacctgatg
     2341 ttttctgccc tgaaagttat cagtgcatcc aggttatgct tcaccattgt ttcccgcaag
     2401 cgctggacac gcatctcgcg cattttggga aagtctaccc tatctaccca atctactgca
     2461 taatacatgt tccatctcct cctgattagt aattattaaa ctgctcttaa agatgattcg
     2521 tgaacccagt attgtacgcc ttatattact gcaatacctg tgccaatcaa taatgcagtt
     2581 atttgggcag ttcgggtttc ctttggcttt ttttgtccgc tttcaggcgg actattttgg
     2641 acaaaccact gtttctaggt catatttcgt ttaaattgat taattacagc cctgccgctc
     2701 tctttaatgt gggttccgct tcctgtggtc gcctggatca ggtccagctc ctttaatacc
     2761 agcagatgcc tttttacctg gctttcgctc atgctaaccc cggaattctc aagcgcttcg
     2821 cacaggcgtt tacgcgtcca tttaaagggt ggagaatcaa gggtcttaag gatacttata
     2881 aaatttaata tttgactttg ttcttccaac cgcctgataa ttgcttcagc atgggcatca
     2941 tttgcatcca tcgtcttgtt tatcttgttc cgtataatac ttagaagttc ttcttcccag
     3001 tcaaaattga gttggataaa atttgccatg tattctgcta cattgcgtaa ctccctcacg
     3061 ttgccaggcc atttatagcg ttcaagttcc tctttaagcg ctttagggaa tttctccaaa
     3121 ttaatcccaa actgattttt tagaatattt atgaataaag gaaatatatc ttccggcctc
     3181 tccctgagag atggaagcct aaggggaagt acactaagcc gataatataa atcctggcgg
     3241 aatagacctt catttaccat tgcctggagg tcacggtttg ttgctgctat aatacgtata
     3301 tctaccggta taacctgcga gcccccaacg ggaagtatct gtcgctcctg tatggccctt
     3361 aatacttttt tttgaatgtt gattgtggca tcgccgattt catccaaaaa aattgtacct
     3421 ttgtgcgcca atgcaaatag tccctttttc cctccctttt ttgctcctgt aaaggctcct
     3481 tcttcgtatc cgaataactc cgactcgcac aggttttcgc ttagggctgc aaagtttaca
     3541 gcaacaaagg cttcctccga ccgccccgaa gcattgtgta tcgcttgagc aataagctct
     3601 ttaccggtac cgctttcacc ctctattaat atcgtggaat cgctttttgc aaattgacgg
     3661 gctttcatta tgagctgctc catttttttt gacttataaa taatattgtt gaacgtatac
     3721 ttggcagtga gacctctttc atgaagagag tgtctgtagc ttttttctat tttttctata
     3781 ttcttaatat cttcgataac tattatggtc ccaaggtcgt ctccaaaact gttattgtct
     3841 atctcaatat gatagctttt atcatcaatt gtaacaaaaa agtccttacc gtaactttta
     3901 cttttatcaa gaatgctttt gatatgagag gaatagttat ccttaatatg aagcagctgt
     3961 tcggccttgc ggttatagaa tgaaattttc ccatcctggt ccagaagcat aatcc
//
LOCUS       DAONUO010000191         3983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_15948_length_3983_cov_3.639511, whole genome shotgun sequence.
ACCESSION   DAONUO010000191 DAONUO010000000
VERSION     DAONUO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15948_length_3983_cov_3.639511"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PK768_07340"
     CDS             complement(<1..1345)
                     /locus_tag="PK768_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HQA60822.1"
                     /translation="MDKIIIKGAREHNLKNIDVEIPRDKLVVITGISGSGKSTLAFDT
                     IYAEGQRRYVESLSAYARQFLGQMDKPDVDYIEGLSPAISIDQKTTSKNPRSTVGTVT
                     EIYDYLRLLFARIGKPHCPNCGKEVSRQTVDQMVDTIMKLPERTRIQILAPVVRGRKG
                     EFVKLFESIKKNGFMRVRVDGELIEIGDEIKLEKNKKHHIEIIVDRIIIRPGVESRLA
                     DSIETALAQAEGLVIVDVIGEQELLFSQNLSCPDCNISLEELSPRMFSFNSPYGACPT
                     CSGLGINMEVDPDLVIPDWEKSVAEGAVAPWSATPGGYYFQMLSAVMNHYGYSVDTPI
                     KDLPDEIVDVLLYGSKEDIEFNYVSKFSKRTRRHTGKFEGVINNLRRRYEEAPEFARD
                     EIEKYMSTKPCPTCKGARLKPEVLAVTVGDKSIAQVTALSIREALKFFNELSLSEL"
     gene            complement(1374..3371)
                     /gene="uvrB"
                     /locus_tag="PK768_07345"
     CDS             complement(1374..3371)
                     /gene="uvrB"
                     /locus_tag="PK768_07345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQA60823.1"
                     /translation="MGKFKVVSEYVPRGDQPKAIRQLAEGVNKGYKFQTLLGATGTGK
                     TFTIAHLIQEVQRPTLVIAHNKTLAGQLCSELKEFFPGNAVEYFVSYYDYYQPEAYIP
                     HTDTYIEKDASINDEIDKLRHSATAALFERRDVVIVASVSCIYSLGSPVDYEKQVISL
                     RPGMEKDRDEVIRKLVDIQFTRNEIDFHRGTFRVRGDILEIFPASFTEKAIRVEFFGD
                     EIDRILEVDTLTGEILGERSHVSIFPASHYSTPKDKLEKAIKSIEEELERRLEELKRQ
                     DKVLEAARLEQRTKYDIEMLREMGYCKGIENYSLHLSGRRPGDPPFTLLDYFPEDYLI
                     IIDESHVTLPQIHAMWGGDRSRKESLVEHGFRLPSAFDNRPLKFEEFEERINQIVFMS
                     ATPGPYELEKSSQVVEQIIRPTGLVDPEVEVRPVKGQIDDLIWEIKLRAQKDERVLVT
                     TLTKRMAEDLTDYLREAGIRTRYLHSEILTLERLQILRDLRLGKFDCLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVEGKVIMYADTITESMARAINET
                     NRRRKIQLEYNKKHGIVPKTVKKAVRDVIEATKVAEESADYLPDKDIRKMSKKELKAL
                     LEDLEKQMKEAAKNLEFEKAAELRDMIFEIRAEAFSVIKAK"
     gene            complement(3535..3732)
                     /locus_tag="PK768_07350"
     CDS             complement(3535..3732)
                     /locus_tag="PK768_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60824.1"
                     /translation="MTREEYLDRERDMLLKAYATASDEEKRDIMLKIMNIDTQSRDGD
                     TYGTRSRGYFTRRRNKIIYIN"
BASE COUNT          915 a         1012 c          856 g         1200 t
ORIGIN      
        1 ccaattcgct cagggaaagc tcattgaaaa actttaaggc ctcacgaatg gaaagggcag
       61 taacctgggc tatggacttg tccccaaccg taactgccag aacttcgggc ttgagcctgg
      121 cacccttaca ggtaggacag ggcttcgtgc tcatatattt ttcgatttca tcccgcgcaa
      181 attcaggagc ttcctcgtaa cggcgcctga gattgtttat tacaccttca aatttccccg
      241 tatggcgtct tgtcctcttg ctaaatttgc tcacgtagtt aaactctata tcttcctttg
      301 aaccgtaaag gaggacatct acaatttcat cgggcaggtc tttaatggga gtgtctacgc
      361 tatatccgta gtgattcatc acggcgctga gcatctgaaa atagtaaccg ccgggtgtcg
      421 cgctccaggg ggccacggct ccctcggcta cggatttttc ccaatcggga atgaccagat
      481 cagggtcgac ttccatattt ataccaaggc cgctgcaggt cggacaggcc ccgtatgggc
      541 tattaaagga aaacatcctg gggctcagtt cctccaggct gatgttacaa tcgggacatg
      601 acaggttctg tgaaaagagc aattcctgct cgccaatcac atctacgata accaagccct
      661 ctgcctgggc cagggcagtt tcgatggaat cggcaagccg cgattctacc cctggcctta
      721 ttatgatcct gtctactatt atttcgatgt gatgcttttt attcttctcc agcttaatct
      781 cgtcaccgat ttctattaat tctccatcaa ctcttacccg cataaagccg ttctttttga
      841 tactttcaaa gagcttgaca aactcgccct tcctgcccct caccacagga gccagtattt
      901 gaatcctggt cctttcgggc agcttcatga tcgtatccac catctggtcg acggtttgcc
      961 tggatacctc tttgccgcag ttgggacagt ggggcttgcc tatgcgggca aagaggagtc
     1021 ttaaatagtc atagatctcg gttacggtcc ccaccgttga gcggggattt ttactcgtag
     1081 tcttctggtc tatcgatatg gccggtgaaa ggccttcaat gtaatccaca tcgggcttat
     1141 ccatctggcc taaaaactgc cgggcataag cggaaaggga ttcaacgtag cgcctctgcc
     1201 cttcagcata tatggtgtca aaagccagtg tagattttcc ggaccccgaa atgccggtaa
     1261 tcaccaccag cttgtctctc ggaatttcca catctatgtt tttaagattg tgctccctgg
     1321 cacctttaat tattatcttg tccatatacc acaatcactc tcgcaaatct atattattta
     1381 gccttaatta cgctgaaagc ttcagccctt atctcgaaga tcatgtccct gagttccgca
     1441 gccttctcga attccaggtt ctttgccgct tccttcatct gcttttcaag gtcttcaagt
     1501 agggctttca gctctttctt gctcatcttt cttatgtctt tgtcaggcag gtaatctgcc
     1561 gactcttccg ctactttggt agcttcaatt acatctctga cagctttctt gaccgtcttg
     1621 ggcactatgc cgtgtttttt gttgtattcc agctgtatct tgcgccggcg gttggtctca
     1681 ttgattgctc gggccatgga ttcggttatg gtatctgcgt acatgatgac cttgccctcc
     1741 acgttccttg ctgccctgcc gatggtctga atgagagagg tttccgaacg cagaaagcct
     1801 tctttgtcag catcgagaat ggccaccaat gacacttcag gcaggtccag gccttccctc
     1861 aggagattaa tgcccactaa gcagtcaaac ttgcccagtc ttaaatcccg aagtatctgg
     1921 agcctctcaa gggtcagtat ttcagagtgc aggtaccgtg tcctgatccc tgcctccctg
     1981 agataatcgg taaggtcttc cgccatgcgc ttggtgaggg ttgtaacaag cacccgttca
     2041 tctttttgag ccctgagttt gatttcccat atcagatcat caatttgtcc tttgaccggc
     2101 cggacttcta cttccgggtc taccaggccg gtgggtcgga tgatctgttc tactacctgt
     2161 gaactctttt ccaattcata ggggccggga gttgcagaca taaatactat ttgattaatg
     2221 cgctcttcaa attcctcaaa tttgagaggc cggttgtcaa aagcggacgg cagcctgaat
     2281 ccgtgctcca ccagggattc ctttctggaa cggtcgccgc cccacatggc gtgaatctgg
     2341 ggcagggtaa catgggactc gtctatgatg atcaggtaat cttccggaaa ataatcaagc
     2401 agcgtaaagg gcggatctcc tggccttcgc cctgaaagat gtaaggaata gttttcgatg
     2461 cccttacagt atcccatttc ccgaagcatt tctatgtcgt acttagtccg ctgctccagt
     2521 cttgccgcct ccaggacctt gtcctgccgc ttgagttctt caagccttct ttcaagttcc
     2581 tcttctattg actttatcgc cttttccaat ttatccttcg gagtagaata gtgggacgca
     2641 gggaaaattg acacgtgact gcgttccccc aagatctctc cggttaaggt gtcaacttcc
     2701 aagatgcggt cgatttcatc tccgaaaaac tccaccctaa tggctttttc cgtaaatgaa
     2761 gccgggaata tctccaagat atcgccccgg accctgaagg tccccctgtg aaagtcaatt
     2821 tcgttgcggg taaattggat atcaaccagc tttcgaatta cttcatccct gtccttttcc
     2881 atgccgggtc tcagggatat cacctgtttt tcgtaatcga caggtgaacc caagctgtag
     2941 atacaggata cgctggccac aatgaccaca tcccggcgct caaaaagggc cgcagtagcc
     3001 gagtgcctga gtttgtcaat ctcatcatta atcgaagcat ccttttctat ataggtgtcc
     3061 gtgtgaggaa tgtacgcctc gggctgataa tagtcgtagt aacttacaaa atattctacg
     3121 gcattgccag ggaaaaactc cttcagctcg ctgcagagct gtcctgccag ggtcttattg
     3181 tgggctatta ccagggtagg ccgctgcacc tcctggatga gatgggctat ggtaaaggtc
     3241 ttgcccgtgc ccgtagcccc aagcagtgtc tgaaacttat agcctttatt tactccctcg
     3301 gccaactgcc gtatggcctt aggctggtct ccccgcggaa cgtattcgga tacaaccttg
     3361 aacttcccca tgttttctcc ttcctagtaa atatatcgga aaaatttgtg atctgacttc
     3421 cttattattt atcagtgtca attataccat atagagtata cagtgaaaag ataaaaaccg
     3481 aaaatcagtt cggataactg cctataaaat gaaaaaccag gcccttttgc ctggttagtt
     3541 gatgtaaatg attttgttcc tgcggcgggt aaaatatcct cttgatctag tgccgtaggt
     3601 gtccccatct cgagattggg tatcaatgtt cattattttt aacatgatgt cccgcttttc
     3661 ttcatcagaa gcggtggcat atgctttcaa caacatatcc ctttcgcgat ctaggtattc
     3721 ttccctcgtc acttgctatc cctcctttta tatattatgc ataattgtat gtatgaagac
     3781 gtgtattgaa aatgttattg agagtgttat ttctttcaca agtattatat catataataa
     3841 actttctttc aagtgtggac tgtttgaaat gtcatatatt ttctaaatac tcttttgttg
     3901 tatagacttg tcattgctgc tatatatttt cccctatatc cgcaaattta ttcaaatgcg
     3961 gaataatatt cttgcatcaa act
//
LOCUS       DAONUO010000192         3954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_16070_length_3954_cov_3.900231, whole genome shotgun sequence.
ACCESSION   DAONUO010000192 DAONUO010000000
VERSION     DAONUO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16070_length_3954_cov_3.900231"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PK768_07355"
     CDS             complement(<1..238)
                     /locus_tag="PK768_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQA60825.1"
                     /translation="MLNLDMSKMKDKKILLVDDEVDILNLLEAVLKKEGFNNIYKTTL
                     GYEAIKMCKQMAPDLIVLDIMLPDIDGFEVCRRLR"
     gene            complement(298..525)
                     /locus_tag="PK768_07360"
     CDS             complement(298..525)
                     /locus_tag="PK768_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60826.1"
                     /translation="MALPLGAGIGLALPAMIVANSRYWIIYPWSYPIMAALGGDFDVF
                     DKGSLIFFLSLFLLLVVFAWGYANFIKRDIA"
     gene            complement(621..1556)
                     /locus_tag="PK768_07365"
     CDS             complement(621..1556)
                     /locus_tag="PK768_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60827.1"
                     /translation="MSGLIIETRALTKTFGKIVVVKDINLRVPKRAIYGFLGPNGAGK
                     STTIRMLLDLIKPTGGEVYIFDKDISKHRMEILGKVGSLVESPSYYANLTAYENLEIS
                     RRILKVDKREIDNALEIVNLSKWKNTKVKAFSMGMKQRLGIAQALIGSRELLILDEPT
                     NGLDPAGVREIRNLITSLPKVLGVTVYISSHILSEVEQIADYVGIIRSGELLFQGTLD
                     ELRARTGSEIVIRATPLDEAQDFIRRMGLEVTKREGKLCVPEGKISPEELNRALVFNG
                     FRVSHLTESTKNLEEIFLELTGGGSDAGLLPTSKQ"
     gene            1773..1961
                     /locus_tag="PK768_07370"
     CDS             1773..1961
                     /locus_tag="PK768_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60828.1"
                     /translation="MKHNFKRIGLALILSLLLYVFAPLATVHAEEITKIVIIHTNDTH
                     ARVFEDEYEGMGLPRYLL"
     gene            1970..3538
                     /locus_tag="PK768_07375"
     CDS             1970..3538
                     /locus_tag="PK768_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HQA60829.1"
                     /translation="MRQENPNTLLVDAGDTLHGLNIAILSRGESIVEFMNAMGYDAMV
                     PGNHDFNYGYQRLIELSEMMDFPLISANIAKDGEPLFEPYIVKEIDGIKIAFIGLSTP
                     ETRYKTHPGNVKDLLFFDPITAASDMVLQLQSKADFIVVLSHLGIDKDSEVTSEMVAN
                     NVEGIDIIIDGHSHSVLPEGKVVGDTLIVQTGEHTKNLGIIEIEFLNKKAVSIKAGLI
                     SKEEARNIQPDEEIAKLISKVEEDYAKITSVKIAEAAVRLDGEREHVRTRETNLGNLV
                     TDAIISGTGADIAIVNGGVIRASIEPGDITVGDVIMALPFADYVVVKEVSGKDVIAAL
                     EHGTSSYPEPKGAFPQVGDMTFQIDITKPLGQRVTDVRIAGEPIDPEKMYRIALTDFL
                     ASGGDEYYMFPQYETLLELSNISELVIDYIKELGYIDVSEEGRIAVIGVPQETRGEET
                     SLQGEPMEIAESANFEKPAAAVQERSYVVKPGDVLWRIAEMFGTTWEKLADYNGLENP
                     HLIFPGQTICIPAI"
     gene            3742..>3954
                     /locus_tag="PK768_07380"
     CDS             3742..>3954
                     /locus_tag="PK768_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQA60830.1"
                     /translation="MEKVLQFLRDSKVFYLATVDGDKPQVRPFGVVSVIDGKLYIQTS
                     KAKEVYKQMIQNPNIAICTMGKDGTWL"
BASE COUNT         1189 a          828 c          755 g         1182 t
ORIGIN      
        1 ctcttaatcg cctgcagacc tcaaagccgt cgatatccgg cagcatgata tcaaggacta
       61 ttaaatcagg tgccatttgt ttacacatct taattgcctc ataacctaaa gtcgtcttat
      121 agatattatt aaagccttcc tttttgagca ctgcctccag caaattcagt atatctactt
      181 catcatccac caataatatt tttttatcct tcattttaga catatctagg tttagcaaca
      241 ttgccacctc agatataaaa ttttttgcac aaaattctac tatagatatt ctattcctta
      301 tgcgatatcc ctttttataa aatttgcata tccccaagca aacactacta gtagcagaaa
      361 taaactgaga aaaaatatca aacttccctt gtcaaataca tcaaaatctc ctcccaatgc
      421 tgccattatg gggtatgacc agggatatat tatccagtac ctgctatttg ccactatcat
      481 tgcaggcaat gccaggccaa tacccgctcc cagaggtagg gccatctgag aacatctcaa
      541 gcttattaca taatttatat gccagcatag gggaaattat ggccatgggg cgggtaaagc
      601 ttcctttctg ctttaagaat tcattgttta gaagttggta aaagtcctgc atcacttcca
      661 ccccctgtca gctctaaaaa gatttcttcc aagttctttg tgctctcggt taaatgagat
      721 acgcgaaagc cgttaaaaac caaagccctg tttagttcct ccggactaat tttcccttct
      781 ggtacgcaaa gttttccttc cctttttgtt acttcaaggc ccattcgcct gataaaatct
      841 tgtgcctcat caaggggggt tgcccttatg acgatttcac tgccggtcct tgctcttagt
      901 tcatctaagg tcccctgaaa tagcaattca ccgcttcgga ttatgcccac atagtctgca
      961 atttgttcca cttcacttaa aatgtggctg cttatatata ccgtcactcc taataccttt
     1021 ggcagactag ttattagatt tctgatttcc cttacaccgg ctgggtcaag gccattggtc
     1081 ggttcatcga gaatcaaaag ttcccgggaa cctatcaaag cttgggcaat ccctaatctt
     1141 tgcttcattc ccattgaaaa agctttaact ttagtatttt tccattttga taagttgacg
     1201 atttccaggg cattgtcaat ttctctttta tccactttga ggattctcct gcttatctcc
     1261 aggttttcat atgctgtcaa atttgcgtag tatgagggcg attctaccaa tgagcccacc
     1321 tttcccaata tctccatccg atgcttgctg atgtctttat caaatatata tacctctccc
     1381 ccagtcggct ttattaaatc caggagcatc cgtattgttg tggatttacc tgcgccgtta
     1441 ggacccaaaa atccatatat ggctctcttg ggaactctta ggtttatatc ttttactacc
     1501 acaatttttc caaaagtctt tgtcaaagct cttgtttcaa taatcaaacc gctcatttta
     1561 agcctcctaa aaatcatcat ctgcaaaaaa atcatcatcc tgtgcaaatt atatagaagc
     1621 cagcttaatt tccaatttaa caatccttaa atttacatta atttgttgca tatatttaac
     1681 tttgtcgctc aatttgttaa aatatgatat aaattaacat ttaccggttc gatagctgca
     1741 agttaaaaaa atatgaatag aaaggtatac ttatgaaaca caattttaaa agaattggcc
     1801 tagcactaat tttaagtttg ttgctttatg tatttgcacc tcttgctacg gttcatgctg
     1861 aagaaataac taaaatcgta atcatccata ccaacgatac ccatgccagg gtttttgagg
     1921 atgagtacga ggggatgggc ttgccaagat atctgctata gtaaaacaga tgagacagga
     1981 aaatcccaat actcttttag tagatgcggg tgatactctg catggattga acatcgcaat
     2041 cttatctaga ggagaaagca tcgttgagtt catgaatgcc atgggatatg atgcaatggt
     2101 cccgggcaat catgatttca attacggtta ccaaagactc atagaactct ctgaaatgat
     2161 ggatttcccc cttatttcag ccaatatcgc aaaagatgga gaacctttat ttgagcctta
     2221 tatagtaaaa gaaatcgatg gaattaaaat agccttcatt ggcctaagca ctccggaaac
     2281 tcgctataag acacatcccg gcaatgtcaa agatctcctt ttttttgatc ccataacagc
     2341 agccagcgac atggtgctgc agcttcaaag taaagcagat ttcatcgttg tactttctca
     2401 cctgggtatt gataaagata gcgaagttac gagcgaaatg gtggcaaata atgttgaagg
     2461 cattgatatt ataatagacg ggcacagtca tagcgtatta cccgaaggca aagtggtggg
     2521 cgatacttta atcgttcaga caggcgaaca cacaaaaaac ttgggcatta tagaaattga
     2581 gtttctaaat aaaaaagctg tttctataaa ggcaggtctt atctccaaag aagaagctag
     2641 aaatatacag cctgatgaag agatagccaa gttgatatcc aaggtggaag aggactatgc
     2701 taaaataact tctgtcaaga tagctgaagc cgcagtgagg cttgacggcg aaagggaaca
     2761 tgtcagaacc cgtgaaacca acctgggtaa tttagtgacc gatgcaatta tcagtggaac
     2821 gggtgccgat atagccatag taaatggcgg tgtaatccgt gcatccattg agccgggaga
     2881 tataaccgta ggtgatgtaa taatggctct tcccttcgct gattacgtag ttgtaaaaga
     2941 ggtcagcggt aaagatgtta tagcagcact tgaacacggc acttcttcct atcccgaacc
     3001 caaaggagct ttcccacaag tcggagatat gacctttcaa atagatatta ctaaacccct
     3061 gggtcagagg gtaaccgacg tccgcattgc cggtgagccc attgatcctg aaaaaatgta
     3121 cagaatcgcc ctcacagact ttttggcctc cggtggcgat gaatactata tgtttcctca
     3181 gtatgaaacc cttttagaat tgtcaaacat cagtgaattg gttattgatt atatcaaaga
     3241 attgggatat atagatgttt cagaggaagg acgaattgca gtaattggtg ttcctcagga
     3301 aacaagagga gaagagacct ctcttcaagg agagcctatg gagatagcag aatccgccaa
     3361 ttttgaaaaa cccgcagccg ctgtccaaga aaggtcttac gtggtaaaac cgggcgatgt
     3421 actttggagg atagcagaaa tgttcgggac cacatgggaa aaacttgctg attataatgg
     3481 gctggaaaat cctcacctta ttttcccggg acaaactatt tgtattccgg caatataaaa
     3541 tcggtattta gcttcataaa tcaagcaagc cttccttatc cttaatcgat attaggaggc
     3601 ttgcaattat atttttgtcg tttattcaat taaattgctg ctccagtgca cattccggtt
     3661 gaattcaata ttttactcgc tatgccaatt tgaggtataa tagtactgaa aattcataag
     3721 agatgaggta ggtagttcat catggaaaag gttcttcaat ttttaagaga tagcaaggtc
     3781 ttttacttgg caaccgtgga cggtgataag ccacaggtgc ggcctttcgg tgtagtttca
     3841 gttatcgacg gcaaattata tatccagact tcaaaagcaa aagaagttta caaacagatg
     3901 attcaaaatc ccaatatagc catttgcacc atgggcaaag atggcacgtg gctt
//
LOCUS       DAONUO010000193         3953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_16074_length_3953_cov_4.510005, whole genome shotgun sequence.
ACCESSION   DAONUO010000193 DAONUO010000000
VERSION     DAONUO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16074_length_3953_cov_4.510005"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..386
                     /locus_tag="PK768_07385"
     CDS             129..386
                     /locus_tag="PK768_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQA60831.1"
                     /translation="MTKKDKLIQKLLSKPSDFNYDEAKSLLSKLGYKEDFRGKTSGSR
                     VAFVNSETGHIIRFHKPHPNNQLKYYQVEQLIEELRKQGAI"
     gene            389..742
                     /locus_tag="PK768_07390"
     CDS             389..742
                     /locus_tag="PK768_07390"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQA60832.1"
                     /translation="MRDVMIYKGYLGSVHYSTKDEIFYGKIEGINDLITYEGSSVSEL
                     KAAFEEAVMDYLELCELNGKEPEKIYKGTFNVRIRPELHKLASEKALMEGKSLNQIVE
                     EAIEQYTAKSDSSTS"
     gene            1110..1265
                     /locus_tag="PK768_07395"
     CDS             1110..1265
                     /locus_tag="PK768_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60833.1"
                     /translation="MKKIYKPIALIIAIVFVLVTIASAFTDPKTGPEQEYLTISPERQ
                     EQLLNLQ"
     gene            1302..1625
                     /locus_tag="PK768_07400"
     CDS             1302..1625
                     /locus_tag="PK768_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQA60834.1"
                     /translation="MPKALLDFYFEREQRDDLLEIEEVWSAEDLAFLTEDEINALSGL
                     VASGRDLYSRGDYSGAVQKYNQALKLAPHIASLYFERGQVHLALGDAKQPWMISMRLF
                     DCCRS"
     gene            1583..3016
                     /locus_tag="PK768_07405"
     CDS             1583..3016
                     /locus_tag="PK768_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQA60835.1"
                     /translation="MDDFDETIRLLPELTVAYLIRSQIHAFLGQAEKALNDLNVVLDI
                     EPSNIVALLERGSIYAALGNWNQVVKDSTAVIEQDKENVQAWGMRAVANQNIANYKQA
                     LEDWNQVERLKGPSLEIFANRGNILFNQGNIDAAANEWTKAIEMDKEQAFLYANRGIA
                     YGRLGKLDLAISDFQQAIRLNPKEYTAYNSLGFIYYLQQEYDQAIENYSKALNNYTLD
                     PKVYYNRASAYRKIVNYQAALNDLNESIRLEPSYVEAYIARAGTYFTMLDFDAAIRDY
                     NQALELDPDNIIANYNRSLTYLQQGAYDQAISDLSALIEKHPDLYLCYQQRALAYFQK
                     NQFESAIRDWKEYLRYDSEDAEVNYRIGLTYLKIDDYDNALTYLSRATELDENLGSAY
                     YQRANLYSLQKKYPEAAKDYTKAIELGIGLDICYFNRGIAYLSENKIAQAIADLEKVL
                     EISDNEVLLTEAQNVLDLIDAEMKSKK"
     gene            3735..3899
                     /locus_tag="PK768_07410"
     CDS             3735..3899
                     /locus_tag="PK768_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60836.1"
                     /translation="MKAKAPAMMKALVTMKVQKQVRKAKMPKNLVQMKLKSKALQINT
                     SILITISGTL"
BASE COUNT         1288 a          701 c          939 g         1025 t
ORIGIN      
        1 taaaaagaaa agtagcaaat gacttttgca tctgctactt tgtctacagt ctgaggtttt
       61 gcaactaaat attagttgca aaacctgttt tgttattgta tcctgattat aaagtattga
      121 ggtgtgcaat gacaaaaaag gataaactga tacaaaaatt gcttagtaaa ccttctgatt
      181 ttaattatga tgaggccaag agccttttgt caaaattggg ttacaaagaa gatttccgag
      241 ggaagacttc aggttcgaga gtggcttttg taaatagtga aacagggcat attatcagat
      301 ttcacaagcc tcatcctaat aatcagttga aatattatca agttgaacaa ctgattgaag
      361 aattaagaaa gcagggagcg atttaattat gagagatgta atgatatata agggttattt
      421 gggctcagta cactatagta cgaaagatga aattttttat gggaaaattg aaggtatcaa
      481 tgatttgata acctatgaag gcagcagcgt tagtgaatta aaagcggcgt ttgaagaagc
      541 ggttatggat tatctcgagc tctgtgagct gaatggcaaa gaaccggaga agatctataa
      601 aggaactttt aatgtaagga taaggcccga acttcataaa ttagcgtcag aaaaagcgtt
      661 gatggaagga aaatctctga accaaattgt agaagaagct atcgaacaat atacagcaaa
      721 gtcagattct tctaccagct aaacagcaac ctttgaaata ttctatatca tctgggacca
      781 ggtcgttaag gaaataatta ggggcgatgt ttttggtttg tttaaatcaa aaggtaaatc
      841 aaaagggact gtcccttttg attttttgaa cagaggaacc gtttctcgtt ttttgataat
      901 tgtttaaaac tatttaaatc gtatataatt ttgtggtttt tcagaataat acaataattt
      961 gtttcgttca cgtcgaaaaa ggacctattc gcgcaaaaat gtaacctttt gcggcagatg
     1021 tattacgtta aagagatagt gtgataaaat aagtcttcga aaaagacatg aaaattttat
     1081 tttaatattc agctattatg gggtgtgtta tgaaaaagat atacaaacca atagccctca
     1141 ttatagcaat tgtatttgtc ttagttacca tagcgagtgc ttttaccgac cctaaaaccg
     1201 gtccggaaca agaatacctg actatttcgc ctgaacgaca ggagcaactt ctcaatttgc
     1261 aatagacttt ggctcggctt gaggatttaa gtatcttgcc cgtgcccaaa gctttattag
     1321 atttttattt tgaacgggag caacgagatg atttgcttga aatagaagag gtgtggtcgg
     1381 cagaggatct ggcattttta actgaggacg aaatcaatgc tttgtctggc ttggtggcca
     1441 gcggaagaga tctgtacagc cggggagatt attccggagc tgtgcaaaag tacaatcagg
     1501 ccttgaaact tgctccccat atagcctcat tatatttcga gagagggcag gtgcatttag
     1561 ctcttgggga tgcaaaacag ccttggatga tttcgatgag actattcgac tgttgccgga
     1621 gctgaccgta gcttacctta tccgaagcca gatacacgca tttttaggac aagcagaaaa
     1681 agctctcaat gatttaaatg tcgtactgga tatagaacca tcaaacatag ttgcccttct
     1741 tgagagagga agcatctatg cggccttggg caactggaac caagtagtaa aagacagtac
     1801 tgccgttata gaacaggaca aggaaaatgt ccaggcttgg ggtatgagag cggtggctaa
     1861 tcaaaacata gcgaattaca aacaggcctt agaagactgg aaccaagtcg aaagattaaa
     1921 gggcccatcc ctggagatat tcgcaaaccg gggcaatatc ctatttaacc agggaaacat
     1981 tgatgctgct gcaaatgaat ggacaaaggc tatagaaatg gataaagaac aggccttcct
     2041 gtatgccaat aggggaatag cttatggccg tttaggcaaa ctggatttgg caatttccga
     2101 tttccaacag gcaattcgcc taaatcccaa ggagtacaca gcctataaca gtcttggttt
     2161 tatttactat ttacagcaag aatacgatca ggctattgaa aactattcca aggcactaaa
     2221 taattatact ttagacccga aggtctatta caacagagct tctgcctacc ggaaaatagt
     2281 aaattaccag gctgctttga atgatttaaa tgagtctatc cgtttagagc catcgtatgt
     2341 tgaagcctat attgcaagag ccggtacata cttcaccatg ctggatttcg acgcggcgat
     2401 aagggactac aatcaagccc ttgagttgga tccggacaat atcatagcca attacaatcg
     2461 atctctgacc tatctgcaac agggcgcata tgatcaagct atttcagacc tttctgctct
     2521 tatagaaaag catcccgatc tttacctttg ctaccagcaa agagctttgg catacttcca
     2581 aaagaatcaa tttgagtcgg caattagaga ttggaaagaa tatctccggt atgacagcga
     2641 agatgcggaa gtgaattacc ggatagggct tacctatctt aaaatagatg attacgataa
     2701 tgccctgact tatctgagcc gggccaccga gctggatgag aacctgggtt cagcatatta
     2761 tcaaagagca aatctatact cattgcagaa aaaatatccg gaagcggcta aggattatac
     2821 caaggctata gaattgggaa tcggcttgga tatttgctat tttaaccgag ggatagctta
     2881 tttaagtgag aataaaattg cccaggcaat tgcagatttg gaaaaagtgc tggaaatctc
     2941 tgataacgaa gtattgctta ccgaggcaca aaatgtgctt gatctaatag atgctgaaat
     3001 gaaaagcaaa aagtaacaag tagataaggg aagaaaagga gtaattttag gaaacgaaca
     3061 agaagcagca gtgactcaga atgctcgaga atacaaagat acaagagaaa tataggcgaa
     3121 taagggacag aaggggatta atgataatgt acggattcgt tgggaaaaga actcgaaaaa
     3181 agactttgcg tataggtttg atcctaatgg gggtattatt gctaattttg gttgcacccc
     3241 tctcacatgc ttatgcgtca gacgacattc cgacacctcc atctgctcct atagaggcag
     3301 acaatcctta cgatggcgcc gacccggtcg gtgacgggag cgggactgat cccattggtg
     3361 atggaagtgg gtctgatccc attggggacg gaagcggagc cggttcgtca gatgatacaa
     3421 gcagcactgg accgtcagat ggcgggagcg aagcagggct gaacgatgaa agcgggccga
     3481 cccctcaggt gacggaagtg gaaccgatcc gtcaggagac ggaaacggaa ccaatccctc
     3541 ggatgacgga aatggtactg acccatcgga ttatgaaaat ggtaccgatc cgtctggtga
     3601 cgataatgga accgacccaa caggggatgc gggtgaaggt aacgaatccg ggcaaagtga
     3661 tgataccgag gaccagtccg gtgatggtat tggtgccggt ggagatgagg atcctgatga
     3721 aaatgaaggt gtagatgaag gcgaaggcac cggcaatgat gaaagcactg gtgacgatga
     3781 aggtgcagaa acaggtcagg aaggcaaaga tgccgaagaa cctggtacag atgaagttga
     3841 aatccaaagc actccagatc aatacctcta tattaattac tatttccggt accctataga
     3901 tagtgtaaaa tagttgtgga gttttaggaa agaaacagag atcaactctc ctg
//
LOCUS       DAONUO010000194         3918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_16244_length_3918_cov_3.331608, whole genome shotgun sequence.
ACCESSION   DAONUO010000194 DAONUO010000000
VERSION     DAONUO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16244_length_3918_cov_3.331608"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PK768_07415"
     CDS             <1..601
                     /locus_tag="PK768_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60837.1"
                     /translation="GAGTTYIIAVAIVFIGASLGTIVGAFTGYFGGWLDEVIMRLNDV
                     LTSFPSILLALVFVSIFGPGSYNIIFALGVLFIPSYARVVRSEFVVLKERDFVKNAEL
                     MGAGSLRIMFVHILPNTKRVLIPALLIGFNNAVLAEAGLSFLGLGVQPPEASLGRMLA
                     EAQSYLLDSPWYAIVPGLMITLTVLGFSLISGGLENEQG"
     gene            588..2213
                     /locus_tag="PK768_07420"
     CDS             588..2213
                     /locus_tag="PK768_07420"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60838.1"
                     /translation="MSKADTLLSIEGLTIGFAVRRKINTVVDHISLEVKKGEILGIVG
                     ESGSGKTVTGLAVMGLLPTSAIILSGRIHFLGKNLLTMEEKELRRLRGGEISMVFQDS
                     MTSFNPLLTIGAQVEEMIRLHSKHTREEYRALTLEALMEVGLTEALEIYNRYPHQLSG
                     GMRQRAMIAMAMIAGPKLVIADEPTTALDVTTQGKILQLLKKMNDKYGTSMVLISHDL
                     KVIQSICDRAIVMKDGRIVESGMAKELFIHPKTKYTRQLVASAPVLFSKSNGEVKETS
                     YNTHNEEQKEILTIEKLNVYYSEKAVGLFGKKGRKQVIKDVTISVKQGETLGIVGESG
                     SGKSTLAKAIVGLNKEIEGVININNDDAKSRLVSMSKPQMVFQDPFGCLNPTKKIGWI
                     LEEPLKLQTNLSKKERIARVNEMIQQVGLTSEYLERYPFQLSGGQRQRAAIAAALIVK
                     PKLVILDEPVSSLDVTIQAQILHLLKELQKKHNLTYIFISHDLNVIFQMCNRVCVIYR
                     GEIVEMKEAKELLYRPQHEYTKELLKSAMVLKT"
     gene            2335..3186
                     /locus_tag="PK768_07425"
     CDS             2335..3186
                     /locus_tag="PK768_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQA60839.1"
                     /translation="MDTMLGILKYVQSFSNETLDSFFEIITMLGEEWFFIILFAIFFW
                     CFNKSFGYKLAFICLTSAAINTIIKEIVKLPRPIGYEGIKSIRVETAEGYSFPSGHTQ
                     QSSSLFATLMIEFKKKWLYFIGALGILLVGFSRMYLGVHWPSDVIGGLIIGILWTMIA
                     IKIFDWSKDRGNPMLLGVFVIPMIILMLFFQTATYYKVVGTLLTLWIGYIIDDKYFSF
                     VTKAVWWKQILKLVIGFLGLILIKVYVKKLLPLTIYSDFLRYALMGIWMTVISPIIYR
                     ALYIEEG"
     gene            3394..3750
                     /locus_tag="PK768_07430"
     CDS             3394..3750
                     /locus_tag="PK768_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HQA60840.1"
                     /translation="MLFVCYPRCTTCQKAKKWLLENGVAFDERHIKENNPTIEELKDW
                     HKMSGLPLKKFFNTSGQLYRQLKLKDKLPTMSDDEQYNLLASDGMLVKRPILIGDDFV
                     LVGFKETDWKAALDIE"
BASE COUNT         1213 a          681 c          907 g         1117 t
ORIGIN      
        1 aggagccgga accacatata tcatagccgt cgcaatcgta tttattggtg cgtcccttgg
       61 aaccatagtt ggtgctttta caggctattt cgggggatgg cttgatgagg taataatgag
      121 gttgaacgat gttttgacct cttttccaag tattttattg gcgttagtgt ttgttagcat
      181 atttgggccg ggaagctata atattatctt tgccctggga gtcttattca ttcctagtta
      241 tgctagagtg gtaagaagtg agtttgttgt tcttaaggaa agagattttg ttaaaaatgc
      301 agaattaatg ggggctggaa gtttacgcat tatgtttgtt catatattgc cgaatacaaa
      361 gagggtatta attcccgccc tgctaattgg atttaacaat gctgtactag ccgaagcagg
      421 acttagtttt ctaggtctgg gcgttcagcc acccgaggct agtcttggac gcatgctggc
      481 agaagcccaa agttatctgc tggattcgcc atggtatgca atagtgcctg gcttaatgat
      541 cacactgaca gttctcggct tcagcctcat cagcggaggt ttggagaatg agcaaggctg
      601 atacactttt gagcatagaa ggtctcacta tcggatttgc agtgcgtcga aaaatcaata
      661 cggtggtgga tcatatatcc ttggaggtaa agaaaggtga aattcttggt atagtaggtg
      721 aatcgggttc aggaaaaact gtgactggtc tggctgtcat gggcctgctt cctacatctg
      781 caataatctt aagtggaaga atacattttc tcggaaaaaa tctcttgact atggaagaaa
      841 aggaacttcg ccgacttaga ggtggcgaaa tatcaatggt attccaggat tctatgacat
      901 cctttaatcc gcttcttacc ataggtgccc aggtagagga aatgataaga cttcattcaa
      961 agcatactag agaagagtat agggcgttaa ccttagaagc attaatggag gtcggattaa
     1021 cagaagcact cgagatttat aatagatatc cccaccagtt atccgggggt atgcgccaga
     1081 gagcgatgat agcaatggca atgattgccg gcccgaaact tgtcattgcg gatgaaccta
     1141 caacggcact ggatgtcaca acacagggca aaatactcca actgctgaaa aagatgaatg
     1201 ataaatacgg aacgagtatg gtcttaattt cccatgattt gaaagtaatc caaagcattt
     1261 gcgatagggc aatcgtaatg aaggacggca ggattgtaga gtccggaatg gcaaaagaat
     1321 tatttattca tcctaaaacc aagtacacca ggcaactggt ggcgtcagcg cccgtgctgt
     1381 tttcaaaatc caatggagaa gtcaaagaga cttcatataa tactcataac gaagagcaaa
     1441 aagaaatact cacaattgag aagctcaatg tatactattc cgaaaaagcc gtcggtcttt
     1501 ttggcaaaaa gggcagaaaa caggtaataa aagatgtgac tatttcggta aagcagggag
     1561 aaactctcgg tatcgtcggg gaaagtggaa gcggaaagtc cacgctggca aaagcgattg
     1621 tgggcctgaa caaggaaatt gaaggggtaa taaatatcaa taatgatgat gctaaaagta
     1681 gactagtatc tatgtccaag ccacagatgg tatttcagga tcccttcgga tgccttaacc
     1741 caacaaaaaa aatcgggtgg attctggaag aacccctgaa acttcaaaca aatttaagca
     1801 aaaaagaacg gattgcaaga gtcaatgaaa tgatacagca ggttggcttg acttctgagt
     1861 atttggagcg gtatcccttt caattaagcg gaggccagag acaaagagcg gccatcgcag
     1921 cggcccttat tgtgaaacct aaactggtca tcctggacga accggtatca tcgttggatg
     1981 ttaccattca ggcacaaata ctccacttgc taaaagaatt acagaaaaag cacaatttaa
     2041 cctatatatt tatttcccat gatttaaatg ttatctttca gatgtgtaat agggtttgtg
     2101 ttatatatcg aggtgaaatt gtagagatga aagaggctaa ggaattgctt tacagacctc
     2161 agcacgagta taccaaagaa ctgctcaaat ctgcaatggt actaaaaaca taactagctg
     2221 cccttgcatc aagctgagcc atgttgggac tgttgtgtga tgtcatggaa agacaaaagc
     2281 gtagcgtgaa ttatatggcc gttacaagtg agagctaaat agaggaggat ccttatggat
     2341 acgatgttag gaatactcaa gtatgttcaa tccttttcaa acgaaaccct agattcgttt
     2401 tttgaaatta taaccatgtt aggagaggaa tggttcttca ttattctttt cgctattttt
     2461 ttttggtgtt tcaataagag ctttggatat aaacttgcat ttatttgtct tactagtgct
     2521 gctatcaata ccataataaa agaaattgtc aagttaccaa gacctatagg atacgaggga
     2581 atcaaatcaa ttagagtaga aactgcagaa ggttattcat ttccaagcgg acacactcaa
     2641 caatcaagct cattatttgc gacattgatg atagagttta agaaaaaatg gctttatttc
     2701 ataggggcat tagggatact tttagttggt ttttcaagga tgtatttggg agttcattgg
     2761 ccgagcgatg ttatcggagg attgattatt gggatattgt ggacaatgat tgcaattaag
     2821 atattcgatt ggtctaagga taggggtaat cccatgctgc ttggagtctt tgtaatacca
     2881 atgattattc ttatgttgtt tttccaaacc gctacttact acaaagttgt tggcacactg
     2941 cttacactat ggattggcta tattattgat gataagtact ttagttttgt aacaaaagca
     3001 gtatggtgga agcaaattct caagcttgta ataggattct taggccttat tctaataaag
     3061 gtatacgtaa agaaactact ccccctgacc atatatagcg attttttaag atatgcttta
     3121 atggggattt ggatgacggt tatatctcca ataatctacc gcgcattata tattgaggaa
     3181 ggttagcggc tcagtagcct gcggcaaaaa gttcgtgttc gatggtcata gcctaatata
     3241 cggattgtgt atttctagat gagcagatga cacaggaaat tgggcgggta tttaatgtaa
     3301 gttgtgacaa ggtttatatc gatggaaaga gattagaaga attgacgaag ctatggtatt
     3361 attatgtaaa acaagggaga atggaggtgc attatgttat ttgtatgtta cccgaggtgc
     3421 actacctgcc agaaggctaa aaagtggctg ctggaaaatg gcgtagcttt tgatgaaagg
     3481 catatcaagg aaaacaatcc cacaatcgag gaattaaagg attggcataa aatgagcggt
     3541 ctgccactga aaaaattctt caataccagc ggacagcttt ataggcaact taagctaaag
     3601 gacaagctcc ccaccatgag cgatgatgag cagtataatc tattggcatc agatggtatg
     3661 cttgtcaagc ggcctattct cattggagat gactttgttt tagtaggctt caaggaaacc
     3721 gactggaaag ctgctctcga catagaataa ctaaaagaac ttaagctcca caggcagtta
     3781 ccatatcagt ctgtggagct tgaaacattt ttggacattc cttttagttt aaatctatgt
     3841 acttagccgt tacgccctcc aatttgttga gttcttcgac taaagcctca acatcctcct
     3901 tattgccgca tggctgca
//
LOCUS       DAONUO010000195         3889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_16368_length_3889_cov_3.588680, whole genome shotgun sequence.
ACCESSION   DAONUO010000195 DAONUO010000000
VERSION     DAONUO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16368_length_3889_cov_3.588680"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..590)
                     /locus_tag="PK768_07435"
     CDS             complement(120..590)
                     /locus_tag="PK768_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQA60841.1"
                     /translation="MLRTVATTSNSRGFTLIELIVSLGLVGLTLTATLSLFSLGHKAY
                     NREDIRLYAQQNVRQAFLWLSTSIRQAKRVDLLSEREIRVTNSNGEQTTFYFQRGVLY
                     RRKNTGTNPIAELSDLRFSYSKDKGFVEILLIVNTTSGDVTIKTKVTPIGLLLN"
     gene            complement(565..924)
                     /locus_tag="PK768_07440"
     CDS             complement(565..924)
                     /locus_tag="PK768_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQA60842.1"
                     /translation="MDKKGYTLIEIIMAVTVLAIVVVPLSTLLSQSVFSNIKSEELMT
                     ATALAQEKMEELKALSFHQVRARIGVYVEEDLKSNDFVFIRRLEVEQESENLIKISVE
                     VQGDSGVVHFATYRGNY"
     gene            complement(991..1470)
                     /locus_tag="PK768_07445"
     CDS             complement(991..1470)
                     /locus_tag="PK768_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQA60843.1"
                     /translation="MNVNFDIHKGCYKSNKGFTLIEVMMALAILGLLSTIAVPNFKNT
                     LARYKLQVAAHELAQNIRITQHKSITEGVSHKIMLDLHNRDNYQLLTGSRGKRIKLPP
                     GVYLDWTTYSEVDKTIVFYPTGAPNRGGTIAITNGKDSLFVIVSVATGRVRIDDTPP"
     gene            complement(1491..2696)
                     /locus_tag="PK768_07450"
     CDS             complement(1491..2696)
                     /locus_tag="PK768_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQA60844.1"
                     /translation="MALYFYKGKSLNGEEISGILNSESKHSVARRIKTQGLVPIKIKE
                     INENSLSYIIRVLFKRASSRDLSVFCRQFGAMLDAGVSVLESLSFIARQTKNRNLHSI
                     YTDICWQLKCGYSLADALRNYPYVFSDVFVSMIEAGEASGNLGAILKTLSDYYSNVSM
                     RHEKVKNSMTYPVLLGLISFGVVNFLTIHVLPVYANMFASYGVKLPIATQMLIWINSH
                     MLQIVLTSLTLFLISLSVILKCTKSEESAYKLDKLKLSIPFIGPLITKNEACKIIRVL
                     NILIASGIPILKALDIASNTAKNRFIKREVQKIKIGLKQGDNLSGLLSYKVFPQIMVK
                     MIAIGEESGTLEDMLDKVAAIYENDVDIMLERMMSLVEPVIIIILTVIISFIVISVIL
                     PMFEIYNLF"
     gene            complement(2708..>3889)
                     /locus_tag="PK768_07455"
     CDS             complement(2708..>3889)
                     /locus_tag="PK768_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQA60845.1"
                     /translation="EVVDDALEVAEKAPIIKLVNTIIQQAVKKNASDIHIEPMEKNVR
                     IRYRLDGELVQIMLINKALHQPIITRIKIMSGLDITKKRFSQDGSFKLTVDNHIVDIR
                     VSTIPTINGEKAVLRLLNRDRFLLSLEQLGFNENQTNSIYKMLGYPHGMILVCGPTGS
                     GKTTTLYSMLNYINKPNKNIITLEDPIEFALPGINQVQINPQIGLSFANGLRAILRQD
                     PNIIMVGEIRDQETANIAIRAALTGHLVFSTLHTNSASGAIVRLLDMGVESYLLASCL
                     VGIIAQRLVRKICPQCKEKHLASESERTFIGTKSKDLKLYNGRGCNYCNQTGYSGRTV
                     IGEIVYIHPAHRKLISRRAPLQQIDKVSRKLGYRNIKQNGIELVKKGITTVEEIMALL
                     Y"
BASE COUNT         1128 a          791 c          657 g         1313 t
ORIGIN      
        1 ctcttctttt gcaaatatgc gacttttata aagtctggtc taaaatctat ccccaaaaat
       61 gatgatttta ataacatttt ccacatcacc aaagcttgtc ttgtaaattg taaaatcttt
      121 tagtttaaca acaatccaat aggtgtaact ttcgtcttaa tagtaacatc tccacttgta
      181 gtattaacga taagaagtat ctcgacaaat cccttgtcct tactatagga aaatctcaaa
      241 tcactcaatt cagctatagg atttgtaccg gtattttttc ttctgtaaag gactcctctt
      301 tgaaagtaaa atgtggtttg ttctccatta ctgttagtta ctctaatttc tctttcggat
      361 aacaaatcaa cacgcttagc ctgtcttata gaggtggata accaaaggaa agcttgcctg
      421 acattctgct gtgcataaag cctgatatct tcccgattgt atgctttatg accaaggctg
      481 aacaggctaa gggtagcggt taaggtcaaa ccaacaagcc ccaatgaaac aattaattca
      541 atgagagtaa acccacggga gttactagta gttgccacgg tacgtagcaa aatgtaccac
      601 cccactatct ccttgtacct caacacttat ttttataaga ttttcacttt cctgttctac
      661 ttccagtctc ctaatgaaaa caaaatcgtt agattttaag tcttcttcaa catacacgcc
      721 aattctagca cggacttgat ggaaagaaag agcctttaat tcctccattt tttcttgtgc
      781 caaagccgta gctgtcatta attcttcgct ttttatgttc gagaatacac tttgggatag
      841 taaagtgctt aacggaacaa caactatggc taaaacagtt acagccataa ttatttcgat
      901 tagagtatag cctttcttgt ccataccttt cccctctgaa gccatcactt ttgcaattga
      961 aaaggatggg aactttgatt gaagattgtg ctacggagga gtgtcatcaa ttcttaccct
     1021 accggtagct accgatacaa taacaaaaag cgagtccttg ccgtttgtaa ttgcgatggt
     1081 cccgcccctg tttggggctc ctgttggata aaacactatc gtcttatcga cctctgaata
     1141 ggttgtccaa tcaagatata ctcccggtgg cagtttgatt cgttttcccc gactacctgt
     1201 caatagttga taattatctc tgttatgtaa atccagcatt attttgtgcg aaacaccttc
     1261 ggttatggat ttatgctggg ttattcgtat attttgagct agttcgtggg cagcgacttg
     1321 tagtttgtat ctagcaagag tatttttaaa gttaggcact gctatagtag ataaaaggcc
     1381 gagtattgct aatgccatca ttacttcaat taaagtaaaa cccttgtttg atttatagca
     1441 tcctttgtga atatcaaaat taacgttcat tgagcttttg cgatgtcccc ctaaaacaga
     1501 ttatagattt caaacatggg caaaattact gatatcacga taaaagaaat tatgaccgtc
     1561 aatattataa taatcaccgg ttcaactagt gacatcattc tttcaagcat gatatccaca
     1621 tcgttttcat aaatagctgc aaccttgtcc aacatatctt ctaaagttcc tgattcctca
     1681 cctattgcta tcatttttac catgatttgc ggaaacacct tatagcttaa aagccccgac
     1741 aaattatccc cctgttttag accgatcttt attttttgaa cttccctttt tatgaaacgg
     1801 ttttttgctg tattgctggc gatatctaag gcttttaata tgggaatacc gcttgcaatc
     1861 aagatgttca aaactctaat aattttgcag gcctcatttt tcgtaattaa tggtccaata
     1921 aagggaattg acaacttcaa tttatccagc ttatatgctg attcttcaga tttagtgcat
     1981 ttcaaaataa ctgacaagga tatcaaaaac aatgtcaaac tcgttaaaac aatttgcagc
     2041 atatgagaat ttatccaaat gagcatttgt gtcgctattg gtagttttac accatatgaa
     2101 gcaaacatat ttgcataaac gggcagtacg tgtattgtta agaaatttac gactccgaat
     2161 gatattaggc ccaataaaac aggatatgtc atagagtttt ttaccttttc atgtcgcatt
     2221 gaaacattag aatagtaatc cgacagcgtt tttaaaatag cacctaggtt tccggatgct
     2281 tctcctgctt ctatcataga gacaaaaaca tctgaaaaaa cataaggata attcctcaaa
     2341 gcatctgcta aagaataacc gcactttagc tgccaacaaa tatcagtata gatactatgt
     2401 aagtttctgt tttttgtttg tctcgctatg aagcttaaac tttccaaaac acttacccct
     2461 gcatcaagca ttgcaccgaa ttgcctgcaa aacacggaca aatccctaga gcttgccctt
     2521 ttgaaaagga ctcttatgat gtatgataaa ctattttcat ttatttcttt aatcttaatc
     2581 ggtaccaggc cttgcgtttt tatccttctt gccactgaat gcttactttc actatttaat
     2641 ataccgctaa tctcttctcc gtttaaactc ttacccttgt aaaaatataa tgccataaca
     2701 tcaaccctta atacagtaat gccattattt cttcaacagt ggtgatgcct ttttttacaa
     2761 gctctatacc gttttgtttg atatttctat aaccgagttt acgagaaact ttgtcaattt
     2821 gttgcaatgg agcccgcctt gaaatcaact ttcggtgcgc tggatgtata tatactattt
     2881 cacctattac cgtgcggcca ctgtaacctg tttgattgca ataattacag cccctaccat
     2941 tatatagttt aaggtccttg ctttttgtcc caatgaaagt tctttcgctc tccgaagcaa
     3001 ggtgtttttc tttacattgg ggacatattt ttcgaactaa tctttgagcg attatgccta
     3061 ctaggcaaga ggctaaaaga taagactcaa cccccatgtc cagaaggcgg acgattgcac
     3121 ctgatgcact atttgtatgt aaggttgaaa aaaccaagtg accggttaga gctgccctaa
     3181 tagctatatt agcagtttct tgatccctta tttctcctac catgatgata ttggggtctt
     3241 gtctcaagat agcccgtaag ccattagcaa aacttaatcc tatttgggga tttatttgca
     3301 cttgatttat cccgggtaag gcaaattcta tagggtcttc aagggtaata atatttttat
     3361 tcggcttatt aatgtaattt aacatcgagt ataaagtagt agttttaccg ctaccagttg
     3421 ggccgcagac cagaatcata ccgtgaggat agccaagcat tttatatatg ctgtttgttt
     3481 ggttttcatt aaaacccaac tgctctaaac tgagtagaaa tctgtctcgg tttaacaatc
     3541 ggagtactgc tttttcccca tttatagtag ggatcgtaga cactctaata tcaacaatgt
     3601 ggttatctac tgtgagttta aagctcccat cctgagagaa tctttttttg gtgatgtcaa
     3661 ggccagacat tatttttata cgagtaatga tgggttgatg cagggctttg ttgatgagca
     3721 ttatttgcac aagctcaccg tctagcctgt acctgatgcg aacatttttt tccataggtt
     3781 caatatgaat atcactagca tttttcttaa ccgcttgctg tattattgta ttgacgagct
     3841 taattatggg tgccttttct gctacttcca aggcatcgtc aacgacttc
//
LOCUS       DAONUO010000196         3855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_16542_length_3855_cov_4.501579, whole genome shotgun sequence.
ACCESSION   DAONUO010000196 DAONUO010000000
VERSION     DAONUO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16542_length_3855_cov_4.501579"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK768_07460"
     CDS             <1..282
                     /locus_tag="PK768_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60846.1"
                     /translation="PKLKLAEDKIYEIDDRKNTILLLNQKIQDTDVNDINAIDEIISI
                     VLGEEAAKEIDAMELSIADYQAIMIGIMAAVTGEDYEVTEARFRKEAGV"
     gene            299..619
                     /locus_tag="PK768_07465"
     CDS             299..619
                     /locus_tag="PK768_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp15 family bacteriophage protein"
                     /protein_id="HQA60847.1"
                     /translation="MYEDWGLIEASFAAQYGIRLREEEDMSWSEFCTLLAGIMPETPL
                     GRVVSIRSENDKEVLKNFTKEQHRIRNEWRSRHTNKVITMDKKEAERQIQIFQEMCKK
                     AFGK"
     gene            704..3454
                     /locus_tag="PK768_07470"
     CDS             704..3454
                     /locus_tag="PK768_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60848.1"
                     /translation="MANESTSVGKIQLDIEISRSSLKRELSKLSSAFNSNFKNMFSQT
                     TNFVKSSLDRMTNSFKTFSQVGTGSTDKISRNINKMNSDLEKTQAKIDETNRKLAQLY
                     AEQDNIIESYRSLPAFTGMTSDESLERMLHADPRFSELTSEITRLENRLQELRASSEA
                     TRNSIQSLGNTTERTNNTTRRATDITRRFSREINRTGQSAKKSSGKIADFSNMIGRTF
                     KSILRRIFIYNLIYKGIRGIIKYTGDALKTNKQFVHSLNLIKTNLMVAFQPIYDFILP
                     ALNALMKGIATTTTYIAAAISSLFGKSYEQSYNAAKSLNNAKKAMDGYGKAAKNAMGQ
                     LAGFDEINVLQTKDDTDLGGGGAGDFEMEMPDTSTIDLSGFDRFREIMASILEPFKLA
                     WANEGQSTIDAMKNAFISVLDLIKSIGRSWAEVWTNGTGQKAVETILQIMQNIFNLVG
                     NLATALKNAWNENEVGISIIQGVLDIFNIILQTIERITESTSEWAKTLDFSPLLESVS
                     ILLEALEPLTQNIGDGLVWLWENALQPLAGWVIEDALPVFLELLAEALNVLNVVLEAL
                     RPLGEWLWEEFLQPIAEWTGGVIISILEGITDKLRDVSKWIEDNQPVVETLAIIIGSF
                     AAAWGLVNVAVGVWNVVGAIATGVTTALGTAIAFLTSPIGIAIAIIGSLIAIGVLLYQ
                     NWDWIKEKAGEVWESIKNIFERFKNWLGDVFATDWSQKFGMFGDILNVFLGSIKDIFD
                     GVKRIFNGIIDFITGIFTGNWKKAWEGLKDILKGVADTFVAIVKAPINGIISLVNGAI
                     SGLNKIKIPDWVPGVGGKGINIPKIPMLAQGGYVRANQPQLAIIGDNKREGEIIAPES
                     KITEAVAAAMSTFTDKIDEMMSSHKVEKRIYKIGESELAEIIIKAINSHQRQIGSTVL
                     EV"
     gene            3457..3810
                     /locus_tag="PK768_07475"
     CDS             3457..3810
                     /locus_tag="PK768_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60849.1"
                     /translation="MVKINNVEITEFLESFEVSISDLDSETTTRTADGTLNRDRIAVK
                     QKIDLSFAPLLWEDMSRILKATENIFFEIYYPNPESGEYETKTVYVGDRKAAVMVKKE
                     EQIMWKGLKFSLIER"
BASE COUNT         1425 a          551 c          844 g         1035 t
ORIGIN      
        1 cctaagctta agctagcaga ggataagata tatgagattg atgatagaaa aaacaccata
       61 cttctcctta atcagaaaat acaggatact gatgtaaatg acatcaatgc tatagatgaa
      121 ataatatcta ttgtattagg tgaagaagca gccaaagaaa ttgatgctat ggaactatct
      181 atagcagact atcaggcaat tatgattgga ataatggctg ctgtcactgg tgaagattac
      241 gaggtcaccg aggctcgatt tcgcaaagaa gcaggagtat aaagagtatt ggtatgactt
      301 gtatgaggac tgggggctga ttgaagcctc ctttgccgct caatatggta tacgtttaag
      361 agaagaagaa gatatgtcct ggagcgaatt ttgcactcta ttagcaggta taatgcctga
      421 aacaccactc ggtcgagtag tatcaattag aagtgaaaat gataaggaag tattaaagaa
      481 cttcacaaaa gaacagcaca gaattagaaa tgaatggaga agtagacata caaacaaagt
      541 gattactatg gataaaaagg aagctgaaag gcagatacaa atatttcagg aaatgtgtaa
      601 aaaagcattt ggaaaataaa agattgaaac actatattat tttaatagaa taagcactta
      661 cataggtagg tgcttgttct atgcctaaaa aggtggtgaa agaatggcaa atgaaagtac
      721 tagtgtaggc aaaatacaac ttgacataga aattagtcgc tcatctttaa aacgagaatt
      781 gagtaagctt agcagtgcat ttaatagcaa ctttaaaaat atgtttagcc aaactactaa
      841 ctttgttaaa agctcacttg ataggatgac gaatagtttt aaaacttttt cacaggttgg
      901 aactggttcc actgataaga tatctagaaa tataaacaaa atgaattcag atttagaaaa
      961 aacacaggct aaaatagatg aaaccaacag aaaattggct caattatacg ctgaacagga
     1021 caatataatt gagtcttata gaagtttgcc tgcatttaca ggcatgacta gtgatgaaag
     1081 cttagaacgg atgttacatg ctgatccaag attttctgaa ttaactagtg aaatcactag
     1141 acttgaaaat agactacaag aattaagggc atctagtgaa gcaacaagaa attccattca
     1201 aagcttaggt aatactacag agcgtactaa taataccact agaagagcta ctgatatcac
     1261 aagacgattt agcagggaaa tcaacagaac tggacaaagt gcaaagaaat catctgggaa
     1321 aatagctgat ttttctaata tgataggacg tacattcaag tccatactta gaagaatatt
     1381 tatttacaat ttaatttata aaggaatacg tggaattatt aagtatacag gtgatgcact
     1441 aaagactaat aaacaatttg tacattccct aaatttaata aaaactaatc ttatggtagc
     1501 atttcaacct atctatgact ttatattacc cgctttaaat gctttaatga aaggaatagc
     1561 aacaacaaca acctatattg ctgctgcaat atcatcttta tttggaaaat cctatgaaca
     1621 aagctataac gcagctaaaa gtctaaataa tgctaaaaaa gctatggatg gatatggtaa
     1681 ggcggctaaa aatgccatgg gccagttagc agggtttgat gaaataaatg tattgcaaac
     1741 aaaagatgat acagatttag gtggtggtgg agccggggat tttgagatgg aaatgcccga
     1801 tacatccact attgatttat ctggatttga taggtttaga gaaatcatgg caagtattct
     1861 tgaaccattc aaattggctt gggcaaatga agggcaatca actatagatg cgatgaaaaa
     1921 tgctttcatc tctgtattgg atttgataaa gtcgataggc aggagttggg cagaggtttg
     1981 gactaatggt acaggacaaa aagcagtaga aacaatactg caaataatgc aaaatatatt
     2041 taatctagta ggcaaccttg ctacagcttt aaaaaatgca tggaatgaaa atgaagttgg
     2101 tataagtatt attcaaggcg tgcttgatat atttaacatt atactgcaaa cgattgaaag
     2161 gattacagaa tcaacttccg aatgggctaa gacattggat tttagtccgt tactcgaatc
     2221 agtaagcata ttattagaag ccttagaacc tttaacacaa aatataggtg atggactggt
     2281 atggctttgg gaaaatgcac ttcaaccact tgcaggatgg gtaattgaag atgcccttcc
     2341 agtattttta gaattgctcg cagaggcatt aaatgttcta aatgtggttt tggaagcact
     2401 aaggccccta ggtgaatggc tgtgggaaga attcctgcaa ccaatagctg aatggaccgg
     2461 cggtgtaata atatccattc tagagggtat aaccgataaa ctaagagatg tatcaaagtg
     2521 gatagaagac aaccagcctg tagttgagac attagccatc ataataggta gttttgcggc
     2581 tgcatggggg ctcgtaaatg tagcagttgg cgtatggaat gttgttgggg ctatagctac
     2641 tggcgtaact acagcattag ggacagcgat agcatttctg acttctccaa ttggcatagc
     2701 aatagcaatt ataggttcac ttattgctat cggagtgctt ttataccaaa attgggattg
     2761 gataaaagaa aaagctggcg aagtctggga aagtataaaa aatatatttg agagatttaa
     2821 aaactggctt ggagatgttt tcgcaactga ttggtctcaa aagttcggta tgtttggtga
     2881 catacttaat gtctttttag gtagtataaa agacatattt gatggtgtta aacggatttt
     2941 taatgggatc attgacttta tcacaggcat attcaccggg aactggaaaa aagcttggga
     3001 aggtctaaag gatatcttaa aaggtgtggc agatactttt gttgctattg taaaggctcc
     3061 gataaatggt attataagtc tggtaaacgg agcaatatca gggctaaata aaataaaaat
     3121 acctgactgg gtgccaggtg taggcggtaa agggataaac atacctaaga tacctatgct
     3181 tgcacagggt ggatatgtaa gagcgaacca accacaacta gccattattg gtgataataa
     3241 gcgtgaaggt gagattatcg cacctgaaag caagataaca gaagcggttg cggctgcaat
     3301 gtctacgttt acagacaaaa tagacgaaat gatgagtagt cataaagtcg aaaaaagaat
     3361 atataaaata ggtgaaagtg agttggcaga gataataata aaagcaataa actcacatca
     3421 gcgtcagata ggaagcacag tgttggaggt gtaggcatgg taaagataaa caatgtagaa
     3481 ataactgaat ttctagaaag ctttgaagtg tcaatatcag acttggactc agaaacaaca
     3541 acaagaacag cagatggaac tttgaaccgt gatagaatag cggtgaaaca aaaaatagac
     3601 ctaagtttcg ctccgttgct ttgggaagac atgtcaagga ttttgaaagc tacagaaaat
     3661 atattttttg aaatatacta ccctaaccct gagtcaggag agtatgagac aaaaacggtt
     3721 tacgttggag acagaaaagc agctgtgatg gtaaaaaaag aagagcaaat aatgtggaaa
     3781 ggtttaaaat ttagcctaat agagaggtga gggtatgtat ccagcaagcg aaatgtttca
     3841 aagacttata caaag
//
LOCUS       DAONUO010000197         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_16578_length_3848_cov_2.828104, whole genome shotgun sequence.
ACCESSION   DAONUO010000197 DAONUO010000000
VERSION     DAONUO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16578_length_3848_cov_2.828104"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..2350)
                     /locus_tag="PK768_07480"
     CDS             complement(323..2350)
                     /locus_tag="PK768_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA60850.1"
                     /translation="MTKKYQHLFSPIKIGNVEVKNRFAMMPMGVFSPRLMNPDGSYSK
                     DGADYYIERAKGGTGLIITGLVPVIGLLGGLHICASPETYIENQKYLVEGIHKYGAKV
                     FIQMTALSGRSSVFLGDPAPSVMPMVWDPTKYTREMTKDEIKQYIENFAKGALYAKKA
                     GIDGVEIHAVHEGYLLDQFTIANFNHRTDEYGGSLENRLRFPIEIVKAIKETCGQDYP
                     VSLRYSVRSYTKGFNRGALPGEDFVEFGRDLEESRVVAKMLVDAGYDMLNCDNGTYDA
                     WYWPHPPTYMPKACNLEDVKEIKKVVDVPVVCAGKFYDPDIAEEAIANGDIDIMGMGR
                     PLLADPYIPKKIMEGRLDDIRPCIGCHQACLSRIFQQKDICCAVNPAVGREKAYALKP
                     SERKKKIAVVGGGLAGMEAARVSAIRGHEVDLYEKTGELGGVFIAAAAFDFKEEDRRL
                     LNWYKKQLADLKVNVKLNTEFTDADKDGYDEIFVATGATENKLDAPGLDSPNVSNAID
                     TLLNRKIENKNVVIIGGGLTGCEIAYELAKKGCTVTVVEKTDTILNTFGLSAANYNML
                     VELMEYYKVKIIKNAVLDKYEDGIAYVTETIKNYPNVAGRAAYPFGVGFGVPNQHKIP
                     ADHIVISVGYTSNKELYEKIKADNVHLIGDAIKPGNVMDAIWSAYEYAMNI"
     gene            complement(2628..3185)
                     /gene="cobU"
                     /locus_tag="PK768_07485"
     CDS             complement(2628..3185)
                     /gene="cobU"
                     /locus_tag="PK768_07485"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HQA60851.1"
                     /translation="MKITFITGGARSGKSKLAENTALKTGQKVIYLATAQALDEEMAN
                     RIKIHQQRRPDNWTTIEEPRFLSRVLKDIRTDRSYESYDIILIDCLALLVSNWLPLEK
                     ASDTSQWDNLRAELLDEIKAMISEMEKIQKDFIIVSNEVGLGIVPEYPLARLYRDLLG
                     EVNQIVAASADEVIFMVSGLPMKLK"
     gene            complement(3200..>3848)
                     /locus_tag="PK768_07490"
     CDS             complement(3200..>3848)
                     /locus_tag="PK768_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HQA60852.1"
                     /translation="VFAFIGSAGTVFLVYSLASIGGRTYSSTLLLSGVAVSSFLSAIV
                     SFFMYFSDGKLHHIYFWLVGSFASQGWNEIYINFPYAIIGFLMAYWNLRYLNILQLGE
                     ETAFFTGVDTERLKKVTLSAAALLTASAVAVSGVIGFVGLIIPHITRMIIGPDHRQLF
                     PLSTLIGGLYLMMADTVSRVIIAPTELPVGVLTALFGGPFFIYLLFQKKNKDYRL"
BASE COUNT         1093 a          855 c          684 g         1216 t
ORIGIN      
        1 tttcatttga ggcagcggta tgcgggtgag aaacaacagt gctgcataaa atcccatgga
       61 aaaaacgctt cctttcaagg tttatcctaa ttcaggtgtt ctagttatta ttatgacaaa
      121 atatgtgttc gacaataagg tttaaaaatc cttcctttaa agtataagtc ttccctttcc
      181 catagaaaaa ctcccctttg cgtagtcctg tatttttgtt gtttcaggta tctactcaaa
      241 agggagcata tcaataaagg cctgtcttcc tatttttctt tttagtgcaa tggtaatttc
      301 ttgattgatg taggtagctc gtttagatat tcatagcgta ttcatatgca gaccatattg
      361 catccatgac attcccgggt tttattgcat ccccaataag gtgaacattg tcagctttaa
      421 ttttttcata gagttcttta ttgcttgtat acccgacaga tattacgata tggtctgcgg
      481 gaatcttgtg ttgatttggt acaccaaaac caactccaaa aggataagca gcacgccctg
      541 caacatttgg atagtttttg attgtttcgg ttacatatgc aattccgtct tcgtatttat
      601 caaggacggc attcttaatt attttgactt tataatattc cattaattct actagcatgt
      661 tatagttcgc agcactcaag ccgaatgtat tcaggatagt atcggttttt tcaacaaccg
      721 ttaccgtgca tcctttcttt gctaattcat atgcaatctc acagccagtc aatccaccac
      781 caattattac tacatttttg ttctcaatct ttctgtttag caaggtatct attgcattcg
      841 acacgttcgg actgtctaaa ccgggtgcat ctaatttgtt ttcggtggcc ccagttgcta
      901 caaatatttc atcatagcca tctttatcag cgtcggtgaa ctcagtgttc agtttaacat
      961 tgacctttaa atcagctaac tgttttttat accaattcag caaacgtcga tcttcctctt
     1021 taaaatcgaa ggctgcagct gcaataaata caccacctag ctcacccgtc ttttcataca
     1081 aatcaacttc atgcccacgt attgctgaga ctcgtgctgc ttccatgccg gcaaggcctc
     1141 caccaacgac agcaattttt ttctttcttt cagacggttt tagagcgtat gctttttcac
     1201 gtccaacggc aggatttaca gcacaacaga tatctttctg ttggaaaata cgactcaggc
     1261 acgcttgatg acaaccaata cagggccgaa tgtcatccaa cctgccttcc attatcttct
     1321 tgggtatgta cggatcagca agcagcgggc gccccatacc cataatgtca atatcgccat
     1381 ttgcaatagc ttcttccgct atatccggat cgtagaactt gccagcacag acgacaggaa
     1441 catcaacaac cttcttgatt tccttaacat cttcaagatt gcaagctttt ggcatgtatg
     1501 tgggtggatg gggccaatac caagcatcat atgtaccatt atcgcagttg agcatatcgt
     1561 aacctgcatc tacgagcatc tttgcaacta cacggctttc ttccaaatca cgaccaaatt
     1621 ctacaaaatc ttctccggga agtgctccgc gattgaagcc ttttgtataa ctccgtacac
     1681 tataacgcaa agaaacggga taatcttggc cacaggtttc tttaattgct ttaacgattt
     1741 caatggggaa gcgcaagcgg ttttccaaac tgccgccata ttcatctgta cggtgattga
     1801 aatttgcaat tgtaaactgg tctaacaggt agccctcatg tacagcatgt atttcaacgc
     1861 cgtcaatacc tgctttcttt gcatagagag caccttttgc aaagttttcg atgtattgct
     1921 taatctcgtc ttttgtcatt tcgcgagtat atttagtggg atcccagacc ataggcatga
     1981 cactcggggc tgggtcacct agaaaaacag aggatcgtcc agagagagcc gtcatctgga
     2041 taaatacctt tgcgccatac ttatgtatac cctcaactag atacttttga ttttcgatat
     2101 aggtctctgg agaggcacaa atgtgaagtc ctcctaacaa accaatcacc ggtacaaggc
     2161 cagtaataat taggccggtg ccccctttgg cacgttcgat ataataatct gcgccatctt
     2221 ttgaatagct gccatcggga ttcattaatc tcggagaaaa aacacccatg ggcatcatgg
     2281 caaatctgtt tttgacctct acattaccaa tcttgatagg agaaaataga tgttgatact
     2341 ttttagtcaa ataaatcaaa tcccctttca ttttttttaa atttttgtcg attcgttatg
     2401 ctaatatatt atcaaatctg tcttgtttaa aatttatcat agtttgaact ttgaatcaat
     2461 gtctttaatc tcgtgtttat tttcagtatt atcgtaataa tggaagtaat agcctttatt
     2521 taccaaggct tataggtttt cattgtttat cgatataata caatcacgtt cccccattaa
     2581 aaaaacaaag gcagatttat ccacctttgt ttttgtttag ctattatcta tttcaacttc
     2641 atcggcagac ctgaaaccat aaaaataacc tcgtcggcag aagctgctac tatttgattt
     2701 acttcgccaa gcaaatccct gtacagcctc gctagcggat actcaggcac tatgccaagg
     2761 ccgacttcat ttgaaacgat aataaaatct ttttgtattt tttccatttc agaaatcata
     2821 gccttgattt catctaacag ttcggctctc aaattgtccc actgtgatgt atctgaagcc
     2881 ttttccaggg gaagccagtt tgaaactaaa agggccaggc agtctatcaa aattatgtcg
     2941 taactttcat aacttctgtc tgtcctaata tctttaagca ccctcgataa gaaacgtggt
     3001 tcttctatag ttgtccagtt atcaggcctt cgctgctgat gaattttgat ccggtttgcc
     3061 atctcttcat ccaaggcttg ggcagttgct aaatatatta ctttttggcc tgtttttaaa
     3121 gccgtgttct cagctaattt gcttttgccg cttctggccc ctccggttat aaaagttatt
     3181 ttcatagtat cgccccatct catagtctat aatccttgtt tttcttttga aagagcaagt
     3241 aaataaagaa aggaccgccg aaaagtgctg taagcactcc tacgggtagt tctgtcggag
     3301 caattattac cctagaaacg gtatctgcca tcattaaata caatccaccg atcaatgtgg
     3361 atagaggaaa caactgcctg tgatcaggcc caataatcat tcgagtaata tgcggaataa
     3421 ttaggccgac aaagccgatt actccgctga ctgccacagc agaagctgta agcagtgctg
     3481 ctgctgacag ggtgactttt ttaagtctct cagtatcaac tcccgtaaaa aatgcggtct
     3541 cctctcccaa ctgaagtata ttaagatacc ttaagttcca gtatgccata agaaatccta
     3601 taatggcata aggaaaattg atatagattt cattccatcc ctgtgacgca aagctaccaa
     3661 caagccaaaa atatatatgg tggagttttc catcactaaa atacataaaa aaggaaacaa
     3721 tagctgacaa gaaactgcta acagcaacgc ctgaaagaag taacgtggaa gaataggttc
     3781 gccctcctat acttgcaagg ctgtaaacta aaaatacggt tcctgcagaa cctataaaag
     3841 caaagacc
//
LOCUS       DAONUO010000198         3825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_16693_length_3825_cov_3.603448, whole genome shotgun sequence.
ACCESSION   DAONUO010000198 DAONUO010000000
VERSION     DAONUO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16693_length_3825_cov_3.603448"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /gene="aroF"
                     /locus_tag="PK768_07495"
     CDS             complement(<1..909)
                     /gene="aroF"
                     /locus_tag="PK768_07495"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQA60853.1"
                     /translation="MIIVMRTDGEARDADAVKDKILDLGLEVCEAIGVNYHIIGMIGD
                     TSSVDAESFKLMEGVEKVIHVQEPYKRVSRLFHPENSVISVNGLSIGGEKLAIIAGPC
                     SVESKSQITEVAIEVKKAGAGFLRGGAFKPRTSPYSFQGLKEEGLEYLKYAKEVTKLP
                     IVVELMSVDKLELYEADVDIIQIGARNMQNFDLLKAVGQTRKPVLLKRGLSATIEEFL
                     TAAEYILAGGNENVILCERGIRTFETYTRNTLDISAVPVIKKLSHLPVIVDPSHASGI
                     WWMVEPLAMAAVAVGADGLMIEVHNDP"
     gene            complement(1326..2594)
                     /gene="guaD"
                     /locus_tag="PK768_07500"
     CDS             complement(1326..2594)
                     /gene="guaD"
                     /locus_tag="PK768_07500"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HQA60854.1"
                     /translation="MKELQIFKGDIIFTPTPQEFVSLEGGYIVVSNGKVEGLYEELPE
                     TFKGVEITDFGRRLIIPAFVDLHVHAPQYYQTGVGMDKELIPWLNSHTFPLERRFDDP
                     EFAREAYSSFVDELIRQGTLRACIYATVHKESTEILFDILASRGIGAFVGKINTDRNC
                     PHYLKEDTSLSLHETEKLIISYNDHPMVKPIVTPRFAPTSTEKLLNGLARLARKYNVP
                     VQSHLSENTQEIEWVKELFPSQATYADVYYYFGLLGQTPTLMAHAIHLEERELELIEN
                     SDVWLVHCPESNINLSSGIMPVRELLDRGTKLGLGSDIGAGHNIALYRVIVRAIQLSK
                     IRCFFEPNVKPLSSPEAFYLGTKGGGSFFGKVGSFEKGFSFDALVVDDKLPETLEFSL
                     EDRLQKFLYTGESDSIIARFVEGKRINCGD"
     gene            complement(2731..3552)
                     /gene="pheA"
                     /locus_tag="PK768_07505"
     CDS             complement(2731..3552)
                     /gene="pheA"
                     /locus_tag="PK768_07505"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQA60855.1"
                     /translation="MGPRGTFTEEALDLWIKKLGVSPEEFKKVPYYSIADMMNAVGKR
                     LDAAVVPIENSLEGSVNITVDSFIRGVGAMIAGEVILPIAQHLLVKKSIPLDEIKEVL
                     SHPQALFQCREFIKRKLPLARLKEVSSTAEAAKFVSESEGNIAAIGSKSLSKIYGLTI
                     AAERIQDSDNNMTRFYVLSTEDEKATGRDKTTMIFSTENKPGSLFRALKVFAERGLNL
                     TKIESRPSRRILGEYVFLVEIEGHRLQEPLKSALIELEHSTSFLKILGSYPIFEF"
BASE COUNT         1030 a          868 c          796 g         1131 t
ORIGIN      
        1 agggtcattg tgcacctcta tcattaaccc atccgctcct accgctactg ctgccatggc
       61 caggggttca accatccacc agattcccga agcatgactg ggatctacga tcactggaag
      121 gtggcttaat ttcttgatta cgggcacggc acttatgtca agggtgtttc tggtgtaagt
      181 ttcaaaggtc ctgatgcccc tctcgcacag aattacattt tcgtttccac ctgccagtat
      241 atattcggct gcagttaaaa actcttcgat tgtggctgaa agaccccgct ttaaaagcac
      301 cggctttctc gtttgaccga ctgcttttaa aaggtcgaaa ttctgcatgt ttctagcgcc
      361 aatttggatg atgtcaacat cagcttcata cagttcaagc ttatccactg acatcaattc
      421 aaccacaatg ggcaatttgg tcacttcctt ggcatacttt aaatactcaa ggccttcttc
      481 cttcaagccc tggaagctgt atggcgaagt cctgggttta aaggcaccgc ctcttaaaaa
      541 acctgcaccc gcctttttta cttcaatggc cacctcggtt atctgggact tgctttctac
      601 cgagcatgga cctgcaatga tggccagctt ctctccgccg atagagaggc cgtttaccga
      661 tatcaccgag ttttcgggat gaaagagtcg gctcaccctt ttgtacggtt cctggacgtg
      721 gatgaccttt tcaacacctt ccatcagctt aaagctttcg gcatccacgc tgcttgtgtc
      781 accaatcatg ccaataatgt gatagtttac gcctatagcc tcacaaactt caaggcccaa
      841 gtccaggatt ttgtctttga cagcatctgc atcccgggct tcaccatctg ttcgcattac
      901 aattatcatt ctaaacacct ccgccatgaa aatattttgc ataacaaaaa agggaatctc
      961 ctccagtgag attcccttta ggtataccta tactaatgag ttttggataa ttatgcggta
     1021 ctaagggtaa aaatcctctt aatacctcta tcattgaatc tgttataaac ctcaccggaa
     1081 ggagggccta ttttattgca gaacaaacaa ccgctaaaat aaaagtaaaa atagtagcgg
     1141 ctaaagttaa agtattgtcc tgcaaagttc cttcctgtga gattttgcat gtgctttgac
     1201 ctccgggcaa gttgtgtttt tggtattttt aacattatat ttaatcgggt attctatgtc
     1261 aaggaaaatt ttttcttttt gatcccaatt ttatgcctaa gggatatata ggccgaaaaa
     1321 agcctttaat ccccgcagtt gattcttttt ccttcaacaa aacgggcgat aatactatcg
     1381 gattcaccgg tgtagaggaa tttttgcagc ctatcttcca gtgaaaactc gagagtttcc
     1441 ggcagtttgt catctacaac aagagcatca aaagaaaagc ccttttcgaa acttcctact
     1501 ttgccaaaaa agctgcctcc tccttttgtc cccaaataaa aggcttctgg gctgctgagt
     1561 ggttttacat tcggttcaaa aaaacatctg atctttgaaa gctgaatcgc ccgcactatt
     1621 actcgataga gggcaatatt atgacctgct ccgatatcgc tgccgaggcc aagtttggtt
     1681 cccctgtcca gcagttcccg gacgggcatg atgccgctgg ataaattgat gtttgactcc
     1741 gggcaatgca ccaaccaaac gtcgctgttt tcgatcagct caagttcccg ctcttccagg
     1801 tgaatggcat gggccatcaa agtgggtgtc tgcccaagaa ggccaaaata ataatagaca
     1861 tcggcataag tggcctggga agggaacaat tcctttaccc actcgatttc ctgggtgttt
     1921 tcagaaaggt gcgactggac cgggacattg tatttccttg caagccgggc caggccattt
     1981 aacagttttt ccgtagaagt gggggcaaag cggggtgtca caatgggttt taccatgggg
     2041 tggtcgttat agctaattat gagcttttca gtttcatgca aggaaagcga cgtatcttct
     2101 ttaagataat ggggacagtt gcgatccgta ttaatcttac ctacaaaggc tccgattccc
     2161 cgagaggcca agatatcaaa caggatttcc gtgctctcct tgtgaaccgt agcatagatg
     2221 cacgcccgaa gagtgccctg gcggattagt tcatcaacaa agctggaata ggcttcccgg
     2281 gcaaattccg ggtcgtcaaa acgcctttcc aggggaaaag tgtggctgtt gagccagggg
     2341 attaactctt tatccatccc aacgccggtc tgataatact ggggagcatg cacgtgcagg
     2401 tctacaaagg caggtatgat taaccgtcgg ccaaaatcgg tgatctcgac tcccttgaaa
     2461 gtttccggca gctcttcata caggccttca actttaccgt tggaaaccac aatatagcca
     2521 ccctcaagac ttacaaactc ttggggtgtt ggggtaaata taatatcccc cttgaatatt
     2581 tgaagctctt tcatagcaga tccccctttc tcgttataag gcctctagta agataaggat
     2641 aaatagtctt taaaaattca tcagcccttt tacacggcct ataataaacg agcttcttac
     2701 atggacaaaa cttttctcaa gttgtattga tcagaattca aatatggggt aggatcctag
     2761 gatttttaag aaacttgtag aatgctccag ttcaattaag gcacttttca aaggttcttg
     2821 cagcctatga ccttcaattt ctactaaaaa aacgtattcc ccaaggattc tcctggaagg
     2881 ccttgattca atcttggtta aattgagacc cctttcggca aaaactttca atgcgcgaaa
     2941 aagacttccc ggtttattct cggtagaaaa aatcatggtg gttttgtcac ggccagtagc
     3001 tttctcatct tctgttgaaa gcacataaaa gcgggtcatg ttgttgtctg agtcttgtat
     3061 tctttctgca gcaattgtca ggccatatat ttttgatagc gacttgcttc cgattgcagc
     3121 gatgttacct tcactttcgg acacaaactt ggccgcttct gcggtgctag agacctcttt
     3181 taacctggct aatggcagct tccgttttat aaattcccgg cattgaaaga gggcttgggg
     3241 atgtgataaa acctctttta tttcatcaag ggggatactc ttttttacca ataaatgctg
     3301 tgcaatgggt agaataactt cgcctgctat catggcaccc acaccgcgga taaatgaatc
     3361 tacggtgatg ttcacggaac cctcaagaga attttctata gggacaactg cagcatcaag
     3421 tcttttcccg acagcattca tcatgtcagc tatcgaataa taaggcactt ttttaaattc
     3481 ttctggggaa actcctagtt tttttatcca aagatcgagg gcctcttcgg tgaaagtccc
     3541 ccttggtccc aaaaatccga tgataatttt ttataccccc attcaagaat gattattgat
     3601 tattttatta caaaaattct aaaattgcat cttaaatttg tttcaagctg atgttgtttt
     3661 tcgaaaactg gagagaaatt gcatatttta gcagttttac agttatatta taaatgatgt
     3721 cgatggacat ataaatactc ataatggggg atatgtatgg ttacaagagg aagtaagaca
     3781 tatgaaaatt tgatgagggc ctttgcggga gaaagccagg cgaga
//
LOCUS       DAONUO010000199         3785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_16901_length_3785_cov_3.936193, whole genome shotgun sequence.
ACCESSION   DAONUO010000199 DAONUO010000000
VERSION     DAONUO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16901_length_3785_cov_3.936193"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="PK768_07510"
     CDS             <1..1369
                     /locus_tag="PK768_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA60856.1"
                     /translation="EYGGSLENRMRFLVECIESARANAGKDFPVIVGLATDHGFPGGR
                     ELDETIEIAKRLEELKIDALHLRRGSYDAMNLIVPTVYMKEGVAVDYAYEVKKAVNIP
                     IIVDGNLKDLSYCERILEEGKTDFIGMGRPFITDPHWPDKARAGKEEDILPCIRCMQC
                     INRVFFARYSACSVNPQFGREHEGQLQPTSKPKRVLIVGGGPAGMTAAKYLAEKGHDV
                     TLVEKSKKLGGHLLEAAVPRYKKDTDGYLEWLIRQLKKPNIRIILDTEVISDFVLNFK
                     ADAVVICTGSVPLLPQVPGIDGENVKLATELLVNYAGDIGDNIVIVGAGLVGCETALF
                     LKEKGKSNITLIDMLPEIAYDVIYLARWSLLEELEQKGIKMKPCLTLKEIKPSGIVVE
                     DREGVLENIEADTVVLATGLEADSSLYDELQGKVDELYRIGDCLAPRKFIDAVHEAYE
                     IAKII"
     gene            1537..1968
                     /locus_tag="PK768_07515"
     CDS             1537..1968
                     /locus_tag="PK768_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQA60857.1"
                     /translation="MKRNLKVIETKIQRLKSYAEKNENIVAVYIFGSYGTKHQHFSSD
                     MDFAVLFKESVTLFEELEIESDISQIFERDDIDLVNLNKAAIDISHQVLYTGDLLFCR
                     DEILLADFKEKVFNIYGDYGIVLKKFYDDYQEGLREEYGGN"
     gene            1955..2383
                     /locus_tag="PK768_07520"
     CDS             1955..2383
                     /locus_tag="PK768_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HQA60858.1"
                     /translation="MAEIDKRRVMEKIRIIQECLNKLRTLSQLSVDEFTSDFKNFDSA
                     KYNLQIAIEAMIDIGNHIISRKGLGTPKTYRDTFEALHKGNIIPKEDLATYKLMARFR
                     NRIVHFYDEVDDREVYKILQNNFADFSSFIEHVSKLLSGT"
     gene            complement(2439..3629)
                     /locus_tag="PK768_07525"
     CDS             complement(2439..3629)
                     /locus_tag="PK768_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HQA60859.1"
                     /translation="MSKKIEVKNLYKIFGSKPKLALKRLKEGWTKEKILKTTGQTVGV
                     NNASFTVDEGEFFVIMGLSGSGKSTLIRCLNLLNRPTAGEILIDGEDIVKYDRQQLRE
                     FRQNKVAMVFQNFGLFTHRTVLGNVEYGLEIRNINKDKRKQIALEALESVGLKGWEDK
                     MPTELSGGMQQRVGLARALANNPDILLMDEPFSALDPLIRRDMQVELLEIQSKLKKTI
                     IFITHDINEAFKMGDRVAVMKDGKIVQQGTPEEILNNPSNEYIEDFVKSIDRTKVIQA
                     KNIMKRPNALVSIKDGVRVAIREMQTNHISSVFVVGENMQLEGIVTIDDCVEAIKNKQ
                     SSLKALLRHDYYKTTEDEYIENLLENATKSRYPIVVVNDENKLLGIIVRTSILSGLLP
                     YNSN"
BASE COUNT         1114 a          693 c          826 g         1152 t
ORIGIN      
        1 cgaatacggt ggaagcctcg aaaatcgaat gaggtttctg gtggagtgca tcgagagtgc
       61 ccgcgccaat gccggtaaag actttcccgt gatagtgggc ctggccacgg accacgggtt
      121 tccgggtgga agggaactag atgaaactat agaaattgca aaaaggcttg aggagctaaa
      181 aatcgatgcc ctgcatttga ggcggggcag ttacgacgca atgaacctca tagtgcctac
      241 ggtttatatg aaggagggcg ttgccgtcga ttatgcctat gaagtcaaaa aggcagtgaa
      301 catacccata attgtagatg gcaacctgaa ggacttatct tactgtgagc ggattttgga
      361 agagggaaaa accgatttta taggaatggg cagaccattt atcacggatc cccattggcc
      421 cgacaaggca agggcgggca aggaagagga tatccttccc tgtatccgct gcatgcagtg
      481 cataaacagg gtgttttttg ccagatactc tgcctgcagt gtcaatcctc aatttggccg
      541 tgagcatgaa ggacagcttc aacctacatc caagcccaag cgagtgctta tagtaggcgg
      601 tggcccggca ggaatgacgg ctgccaagta cttggctgaa aagggccatg acgttaccct
      661 tgtcgaaaag agtaagaagc ttggcggtca ccttctggaa gcagctgttc ccaggtacaa
      721 gaaggatacg gatggatatt tagagtggct gataagacag ctgaaaaagc ccaatataag
      781 aataatcctt gacactgaag ttatatctga ctttgtactc aattttaaag ccgatgcggt
      841 ggttatatgt accggctccg tgcccctact gccccaagtt cccggaattg acggggaaaa
      901 tgtaaagctc gctacggagc ttttggtaaa ctatgctggt gatataggtg acaatattgt
      961 aattgtgggg gcaggattgg taggctgtga aacggccctg tttttaaagg aaaagggcaa
     1021 gagcaatata accttaatag atatgctccc ggaaatcgcc tacgatgtga tttacctggc
     1081 gagatggtcc cttttagagg aactggagca aaagggaatc aaaatgaagc cttgtttaac
     1141 attaaaagaa ataaaaccct ccggaattgt agttgaggat agggagggag tacttgaaaa
     1201 tatcgaggct gatacggtag tcttggccac tggccttgag gcagatagca gtctctacga
     1261 tgagctgcag ggcaaggttg acgaattata cagaatcggt gactgcttag cccctcgcaa
     1321 atttatagat gcggttcacg aagcctacga aatagcaaag ataatttagc tgccaggcag
     1381 cattattaac ttattcaacc ttcgcacgaa ggcccttcct agcgccccta ttcttcaagg
     1441 ggtgttttta ttttgaaaag aagttcccat gatttgttta aatctggaag atttctgata
     1501 taatgaaaaa agaagctgtt ttttacaggt gataatatga aaagaaattt aaaggttata
     1561 gaaacgaaga tacaaaggct gaaatcatat gctgaaaaaa atgaaaatat agtggccgtc
     1621 tatattttcg gctcttacgg tacaaagcat cagcatttta gtagcgatat ggattttgcg
     1681 gtattgttta aggaaagtgt cacgctattc gaagagcttg agatagaatc agatataagc
     1741 cagatatttg agcgggatga catagatctt gtgaacctga acaaggcagc tattgatata
     1801 agtcatcagg tactgtatac cggcgattta cttttttgta gagatgaaat tttgcttgct
     1861 gattttaaag aaaaggtatt taacatttac ggtgactacg gaatagtgct taagaaattt
     1921 tatgatgatt atcaggaagg attgagggaa gaatatggcg gaaattgaca aaagacgggt
     1981 gatggaaaag attaggatta ttcaagaatg tctaaacaaa ttgcgcactc tgtcccaact
     2041 atccgtggat gaatttactt ctgattttaa aaatttcgat tcagcaaagt acaacctgca
     2101 aattgccatt gaagcaatga tagacattgg aaatcatatt atatctcgaa aagggctagg
     2161 aactccaaag acctatcgtg atacctttga agctttgcac aaagggaata ttatacctaa
     2221 agaagattta gcaacctata aattgatggc caggttcaga aataggattg tgcattttta
     2281 tgacgaagta gatgataggg aagtatataa gatacttcaa aataattttg ctgattttag
     2341 ttcttttata gaacatgtaa gtaaactatt atcaggcact taacttattc aaaaagagca
     2401 cccggtaaat atggtgctct ttagtcatta aagagaattt aatttgagtt gtagggcaag
     2461 aggccggata gtatggatgt gcgcacaatt atacctaaaa gcttattttc atcatttact
     2521 acaactatgg gatatcttga ttttgtagca ttttcaagaa ggttttcgat gtattcatct
     2581 tctgtggtct tatagtaatc atgccgcaac aaggctttta gtgaagactg tttgttcttt
     2641 attgcttcta cgcagtcatc tatagttaca ataccttcaa gttgcatgtt ttctcccact
     2701 acaaaaacac ttgaaatatg atttgtctgc atttctctta tagcgaccct cacaccgtct
     2761 tttatggata ccagtgcatt aggtcttttc atgatatttt tggcttgaat tacctttgtt
     2821 ctatcaatgc tcttaacaaa gtcttctata tattcatttg aggggttatt tagtatttct
     2881 tcaggggttc cctgttgtac aattttccca tctttcataa ctgccactct atcgcccatt
     2941 ttaaatgctt cgtttatatc atgggtgatg aaaattattg tctttttaag ttttgattgg
     3001 atttccaaaa gttcaacctg catatctctg cgaatgagcg ggtctaaggc gctaaaaggt
     3061 tcatccataa gcagtatatc cggattgttg gcaagggctc gggcaaggcc caccctttgc
     3121 tgcattccgc cactgagctc agtgggcatt ttatcttccc agcctttaag ccccacactt
     3181 tctagagctt caagggcgat ttgctttctt ttgtctttat tgatattcct gatttcaagg
     3241 ccatattcta cattgccaag gactgttcta tgtgtaaaca gaccaaagtt ttgaaatacc
     3301 atggcaactt tgttttgcct aaattcccgt agttgttgcc tgtcgtactt aactatatct
     3361 tcaccatcta ttaaaatttc acctgcagtt ggcctattta aaagattaag acatctaata
     3421 agtgtggatt ttccgctgcc ggaaagcccc attattacaa agaactcacc ttcgtcaact
     3481 gtaaatgaag cgttgtttac accgacagtt tgacctgttg tttttaatat tttttctttt
     3541 gtccagccct cttttaatct tttaagagca agttttggct ttgaaccaaa tattttatat
     3601 aagtttttaa cttctatttt ttttgacatt atgcaattct ctcccaagga ttttatacat
     3661 gttcatattt tttggaaatc atttgagtaa gcctgtcgat aataatggca agaaagacta
     3721 tactaatgcc tgagttaaag cccatggcaa tatctgttct gttgatcgcg atcagtacat
     3781 tttca
//
LOCUS       DAONUO010000200         3744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17135_length_3744_cov_3.518027, whole genome shotgun sequence.
ACCESSION   DAONUO010000200 DAONUO010000000
VERSION     DAONUO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17135_length_3744_cov_3.518027"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..523)
                     /locus_tag="PK768_07530"
     CDS             complement(8..523)
                     /locus_tag="PK768_07530"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQA60860.1"
                     /translation="METSTIYWSYLHWKGKKMHLAASPKGLCCILWPSEPFEALEKWV
                     RQQFPKASLVQSQEKLALYAKQIEAYLNGQRKSFDIPLDLRGTDFQRAVWQALLEIPY
                     GTTKAYSEIAERIKRPRAVRAVGAAIGANPVPIVVPCHRVIGKDGSLRGFAGGLDIKQ
                     QLLKIEGTLSE"
     gene            complement(602..2212)
                     /locus_tag="PK768_07535"
     CDS             complement(602..2212)
                     /locus_tag="PK768_07535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQA60861.1"
                     /translation="MDTNLKDKNKGGKKLDLNLTASTRGIILLLLILAVVSVSLYAPI
                     KTLTIGDKPFGPDIIYADVIRQEVLLILSIGSVSIFFLTIFAFAMPYSHQRQASMVQF
                     YNKIYLEIKFLIWFIFLGIFFSGLTVIINVGSTNFEKIIHEANWYFYAIGIPVTFVFY
                     TLIYLSLVYLKHIHHTGFVNEIVNNTVIGKLCVYVSKYVKNTLVQIMDEEISRQHREK
                     FILLIIINFVVLMLINLAGPVLGTLLVIGYSAVLFNYGIKLLEKVSALNAASSQLAKG
                     DFDLNLPEDMGILSSFANNLKNIKEGFKVAVEQELKSQNMKTQLITNVSHDLKTPLTS
                     IITYVDLLKKEDLDPDTRREYIDILDKKSKRLQVLIDDLFEASRASTGNIELSPEELD
                     IVALLRQTLGEMEEKINESSLTLRVNLPEHKIICNLDGARTFRVFENVLGNILKYSMP
                     NSRVYIDALEDEKSVSLTFKNISAYEMNFHPSEITERLVRGDRARHTEGSGLGLAIAK
                     SLVELQGGSLHIDIDGDLFKLTITFPKV"
     gene            complement(2190..2876)
                     /locus_tag="PK768_07540"
     CDS             complement(2190..2876)
                     /locus_tag="PK768_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA60862.1"
                     /translation="MYNILVAEDEKEIAAAIEVYLKNQNYGVFKAYNGEEALEIFKNN
                     EIHLVLMDIMMPKMDGLEAVMKIREISNVPIIFLTAKSEDVDKILGLDIGADDYITKP
                     FNPLELLARINSNIRRYTNYSSPPRTEEHLIQIGGIQMDDLKKQVTVDGRPINLTPLE
                     YKILHFLMKHPNRVFSIEEIYESVWQEPAYNPDTVTVHIRRIREKIEINPKEPKYLKV
                     VWGIGYKFEG"
     gene            complement(3052..>3744)
                     /locus_tag="PK768_07545"
     CDS             complement(3052..>3744)
                     /locus_tag="PK768_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60863.1"
                     /translation="GNELSNTDHICSLFNDYGGPRSGQEELALQEVNERNGLAFMNHP
                     GRYTRPVDWYANLYKKYYKEPLIGMEVHNQGDRYPNDRQTWDRVNALVMPEVIVFGYS
                     NDDMHNQSNLFKSYQFMLMRELTVEDLREAMLKGAFYFCYEPGGSGSEDPPVPRISSI
                     EVTDNGTDIKITATTANSITWKTDKGIIANGDFIDLDDINIDGAKFVRAELTNGKGTT
                     YTQPFVLMYSLN"
BASE COUNT          917 a          874 c          777 g         1176 t
ORIGIN      
        1 gacgtaatta ctcggataaa gttccttcta ttttcaaaag ctgctgcttt atatcaagac
       61 ccccggcaaa acccctgagg ctgccgtcct ttcctatcac cctatggcag ggcaccacta
      121 tgggcaccgg atttgccccg atggcagcgc ccactgcccg gaccgccctg gggcgcttga
      181 ttctttctgc tatttccgaa tatgctttcg tagtcccgta gggtatctcg agaagtgcct
      241 gccaaacggc cctttggaaa tccgtgcccc gaaggtcaag gggaatgtcg aagcttttcc
      301 tttgaccgtt taggtatgct tcaatttgct ttgcataaag ggccagcttt tcctggcttt
      361 gcaccaatga tgctttgggg aactgttgcc ttacccattt ttccagggct tcaaagggtt
      421 cactgggcca gaggatgcag cacaagcctt ttgggcttgc ggcaaggtgc atttttttgc
      481 ccttccaatg aaggtacgac caataaatgg ttgaggtttc caatactatc ccctccttcg
      541 aggctgtttt ttaaaactgt cctattttgt acattatatc acttcggtct catgcagaaa
      601 atcacacttt cggaaatgta atcgtcagtt taaacaaatc cccgtcgata tcaatgtgaa
      661 ggcttccccc ttgaagttcc accaggctct ttgcaatggc aaggccaagg cccgaacctt
      721 ccgtatgcct tgctctgtca ccccgcacca ggcgctcggt tatttcggag ggatgaaagt
      781 tcatctcgta ggcggaaatg ttcttgaatg tcaagctgac gcttttttca tcttccagcg
      841 catctatata cactcttgaa ttgggcatgg aatacttgag aatgttgccc aagacatttt
      901 caaagaccct gaaggtcctg gccccatcca aattgcagat aatcttgtgt tcaggaagat
      961 tgacccgcaa tgtcagactg ctttcgttga ttttttcttc catctcacct agagtctgcc
     1021 taaggagggc cactatatcc agctcttccg ggctgagctc gatattgccg gtgctggccc
     1081 ggctggcctc gaacaagtca tcaattaaca cctggagtct ttttgatttc ttgtcaagga
     1141 tgtcaatata ttccctcctg gtatccgggt ccaggtcctc tttttttaac aggtccacgt
     1201 aagtaattat tgaagtgagg ggcgtcttta gatcgtggga gacattggtg atgagctggg
     1261 ttttcatgtt ctggctttta agctcctgct ctacggccac cttaaagccc tctttgatat
     1321 tctttaagtt gtttgcaaag gaacttagga tgcccatgtc ttccggcaag ttgagatcaa
     1381 aatccccctt ggccaattgg ctgctggccg cattcagtgc actaactttc tccaatagtt
     1441 ttattccgta gttaaacagc actgccgaat agcctatgac cagcagcgtt cccaagaccg
     1501 ggccagctaa attaatcaac atcagcacaa caaagtttat tatgattagc aaaatgaact
     1561 tttccctgtg ctgcctgctg atttcctcat ccatgatttg aactagagtg tttttgacat
     1621 atttgcttac atatacgcac agtttcccta ttacagtgtt gttcactatt tcatttacaa
     1681 aacctgtatg atggatatgt ttgaggtata ctaaggatag ataaatcaag gtatagaata
     1741 caaaggttac aggaatgcct atcgcgtaaa aataccagtt tgcctcatga attatttttt
     1801 caaaattcgt gcttccaacg ttaatgatta cagttaaacc tgaaaagaat atcccaagaa
     1861 atataaacca aatcaaaaat tttatttcca ggtatatttt gttataaaat tgtaccatcg
     1921 atgcctgcct ttgatgtgaa tatggcattg caaatgcaaa gatcgtcaga aagaagatgc
     1981 tcacacttcc tattgacagt atcaataaaa cttcctgtct gatgacatct gcgtagataa
     2041 tatccgggcc aaaaggcttg tcgcctattg taagggtttt tatgggtgcg taaaggctca
     2101 cagaaactac ggctagaatt aaaagcaaca aaatgatgcc cctggtacta gctgtaaggt
     2161 ttaagtcgag ttttttgcca cccttattct tatccttcaa atttgtatcc aattccccac
     2221 accaccttca agtattttgg ctctttagga tttatttcta ttttttccct gattcgccgt
     2281 atgtggacgg ttacggtatc cggattgtag gcaggctcct gccacacact ttcgtagatt
     2341 tcctcaatgg aaaaaaccct gttgggatgc ttcatcagaa agtgaaggat tttgtattct
     2401 aaaggagtta gattaatagg ccttccgtcc accgttacct gtttttttaa atcatccatc
     2461 tgtattccgc cgatttgtat caggtgttcc tctgtccttg gaggagaaga atagttggtg
     2521 taccttcgga tgttggaatt tatcctggcc agcagttcca gtgggttaaa gggtttcgtg
     2581 atgtaatcat ccgccccaat gtctaggccc aatattttgt ccacatcttc cgacttggcg
     2641 gtgagaaaga taatgggcac gttactgatt tcccttatct tcattacagc ttccaagccg
     2701 tccattttgg gcatcatgat gtccatcagc accagatgga tttcattgtt cttgaatatt
     2761 tccagggctt cctccccgtt gtaggccttg aaaacaccgt aattttggtt tttcaggtag
     2821 acctctatgg cagcggctat ttccttttca tcttccgcca caagaatgtt gtacacagca
     2881 tcacccctat cccttgaaaa catctatctg tatcatagca cagatttttt aagaaattat
     2941 gatgggaatt cttaaaaatt tattaagaaa aactatatcc ttataaaaaa ataacccctt
     3001 cctccgaata tggcggaagg ggtgagcgat attcgccctg gatgttatta tttaatttaa
     3061 gctatacata agcacaaagg gctgggtata cgttgtgcct ttaccattgg tcagttctgc
     3121 cctgacgaat ttggccccgt ctatgtttat gtcgtccagg tcgataaaat cgccgtttgc
     3181 tattatcccc ttgtcggttt tccaggttat tgaattagca gtggtagcgg ttatctttat
     3241 atcggttccg ttgtccgtca cttcaatact gcttattctc gggaccgggg ggtcttctga
     3301 gccgcttcct ccaggttcat agcaaaagta aaaggctccc ttgagcatgg cttcccttag
     3361 gtcttccacg gtcaattctc tcatgagcat gaactgatag cttttaaaca gattgctttg
     3421 gttatgcata tcatcgttgc tgtatccgaa aacaattact tccggcatta ccagggcgtt
     3481 caccctatcc cacgtttgtc tgtcgttcgg atatctgtcc ccttgattgt gaacttccat
     3541 gcctatcagg ggctctttgt aatatttttt gtaaaggttc gcataccagt caacgggacg
     3601 agtgtatcgc cccggatgat tcatgaaagc aaggccattc cgttcattta cctcctgcag
     3661 tgcgagttct tcttgaccgg atcgtggtcc gccgtaatca ttgaaaaggc tgcagatatg
     3721 atccgtattg gaaagctcat tgcc
//
LOCUS       DAONUO010000201         3741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17150_length_3741_cov_4.223006, whole genome shotgun sequence.
ACCESSION   DAONUO010000201 DAONUO010000000
VERSION     DAONUO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17150_length_3741_cov_4.223006"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PK768_07550"
     CDS             complement(<1..260)
                     /locus_tag="PK768_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HQA60864.1"
                     /translation="MREKAIVVETMRNHARVEIRKSAACDGCKGCSVGREGKPLKVWA
                     KDPIGVKVGQIVEIELSAKTFLSATLIAYGIPLLAFLLGIFLA"
     gene            complement(289..576)
                     /locus_tag="PK768_07555"
     CDS             complement(289..576)
                     /locus_tag="PK768_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HQA60865.1"
                     /translation="MKHGHSCSYSENKANLLRRLRRIEGQIKGIQRMIENEKYCADIL
                     IQIAAVRAALNKVGLIIFEDHTRGCVTEAIQSDIGDKKIHELIDVLSKFIK"
     gene            complement(640..1326)
                     /locus_tag="PK768_07560"
     CDS             complement(640..1326)
                     /locus_tag="PK768_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HQA60866.1"
                     /translation="MKEQFYSRTELLIGKDGIERLNKSRVAVIGIGGVGSFAAEALAR
                     AGVGTLVLVDHDIICPSNLNRQILALRSTLALPKVEVMKNRILDINPDAKVIGIREFY
                     SEKTATRILSTDYDYVVDAIDSLGSKVDLIIRCKQTNIPIISAMGAGNKLHPTKFQVA
                     DISETSVCPMARKIRKELRKNGIESGVKVVFSTETPLINHRPPGSISFVPSVMGLIIA
                     GEVINYLIKN"
     gene            complement(1356..1540)
                     /gene="ssrS"
                     /locus_tag="PK768_07565"
     ncRNA           complement(1356..1540)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK768_07565"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1625..3397)
                     /gene="aspS"
                     /locus_tag="PK768_07570"
     CDS             complement(1625..3397)
                     /gene="aspS"
                     /locus_tag="PK768_07570"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQA60867.1"
                     /translation="MQMNIKRNFYCGNLRPEHEGKEVCLAGWVQTRRDLGGLIFVDLR
                     DRSGVVQVVFNPEQKEAFETADKLRTEYVIAVKGKVVKRPEENVNPKISTGYIEVIGE
                     EVKILSASKTPPFAIEDDVKVDESIRLKYRYLDLRRPKMQHNMIFRSKVKKAIRDFLD
                     EKGFLEIETPILTKSTPEGARDFLVPSRLNPGQFYALPQSPQLFKQILMVSGMERYYQ
                     IARCFRDEDLRADRQPEFTQLDIEMSFVDAEDVISINEQLIKYVVERVTGEQIEIPFP
                     RMTYKDAMEKYGSDKPDTRFGLEMVDVSDLVENADFKVFSQAVKGGGCVKGINIKKGN
                     ESFSRRQIDELVEKSKEFGAKGLAWINITSEGVKSPIAKFFDEETLKQIIDRLEGEID
                     DLLIFIADTNAELVMTSLGNMRLYLGKVLDLIDKDKLNFLWITEFPLLEYSEEEKRFV
                     ARHHPFTSPMEEDIPLLDKDPAKVRSKAYDVILNGTELGGGSIRIHDAELQKKMFEVL
                     GISEERASQNFGFLLKAFEYGVPPHGGIAYGLDRFIMLLLGLESIRDCIAFPKTQNAS
                     CLMTQAPSEVEAKQLKELHIQVKL"
     misc_binding    complement(3475..3680)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          998 a          884 c          631 g         1228 t
ORIGIN      
        1 gcaaggaaaa ttcctaataa aaaggccagc agaggaatac cgtaagctat cagtgtagct
       61 gataaaaacg tctttgcact aagttctatt tccacaattt gtcctacttt tacaccaatt
      121 ggatcctttg cccagacctt aagaggttta ccttctcggc ctaccgagca acccttgcag
      181 ccatcacagg cagcagattt tcttatttcc acccgagcgt ggtttctcat agtctccact
      241 acaatagctt tttccctcat tttatcctcc aggacatttg caataagctc attttataaa
      301 cttgcttaat acatcaatca gttcatggat ttttttatca ccgatgtctg attgtatagc
      361 ttctgtcaca catccccttg tatggtcttc aaaaataatt aaacccacct tatttaaagc
      421 tgccctaacg gctgcaatct gaatcaatat atccgcacaa tatttttcat tctcaatcat
      481 tctttgtatt cctttaatct gaccctctat ccttcttaaa cgtctcagaa ggttggcctt
      541 gttttcagaa tatgaacagc tatgtccgtg cttcaatcca attacctcca ataccatatg
      601 ggggtatcag attaattata tattattatt tcggcataat caattcttta ttaaataatt
      661 tattacttca ccggcaataa taagccccat taccgaaggt acaaacgata tactgcctgg
      721 gggacgatgg ttaattaaag gggtctcagt tgaaaacacc acttttactc cactttctat
      781 accgtttttc cttaactctt tcctaatttt cctggccatt gggcataccg aagtttctga
      841 tatgtcagcc acttgaaatt tggtaggatg taatttgttt ccggctccca tagctgatat
      901 aattggaata tttgtttgtt tacacctgat aattaaatca acttttgaac caaggctgtc
      961 tatggcatcc acaacgtaat catagtcagt agacagtatt ctggtagccg ttttttcaga
     1021 ataaaattcc cggatgccaa tcactttagc atcgggattt atgtcgagaa tcctattttt
     1081 cattacttca acttttggca aagctaaagt tgaacgtagt gccagaattt gtcggtttaa
     1141 gttagaaggg cagataatat catgatctac taatacgagt gtgccgactc ctgctcgtgc
     1201 aagagcttcc gctgcaaatg aacctactcc accgatacct atgacagcta ctcttgattt
     1261 attcagtctc tctatgccat ctttgcctat tagtaattca gttctggaat agaattgttc
     1321 tttcaaaaaa atcgctccat acttttaagt ataaaaaaac ccccaccatg ccgtgttcgc
     1381 tgactgtaat agagacccgg ctacccaggt gggtgtcctc ctaaatgctt tatatttccc
     1441 tcccgaagga gggcatatat gccgcattca gagaccggac tccctatatc agagtgttgg
     1501 ctctttactt agccagttta cacgcacaca gcagggttat tcatttctag cactatatta
     1561 tcacaaaagc tgctatttat caaggtttta cacaaatata gccatgccaa atagtgcttt
     1621 atttttataa ttttacctga atgtgaagct ccttaagttg ttttgcctcg acttcgctgg
     1681 gtgcttgtgt cataagacat gatgcatttt gggttttggg gaaagcaatg caatctctaa
     1741 tgctttctaa tcccagaagc aacataataa aacggtcaag gccatacgct attccaccgt
     1801 ggggtggcac gccatattca aaggccttca gcaaaaatcc aaaattttga ctggctcgtt
     1861 cttccgaaat acccagtact tcaaacatct tcttttgaag ctctgcatca tgaattctaa
     1921 tacttcctcc gccaagttcg gtgccgttta atataacgtc gtaagctttc gatctgacct
     1981 tagcagggtc cttgtccaaa agaggtatat cctcttccat cggtgatgta aaggggtggt
     2041 gcctggctac gaacctcttt tcttcttcgc tatattctag taatgggaac tcggtaatcc
     2101 acagaaaatt taacttatcc ttgtctatta aatcaaggac cttgcccaga tacagtcgca
     2161 tattacctaa tgaagtcatg acaagttcgg catttgtatc cgctataaag ataagcaagt
     2221 catcgatttc gccttccagt ctatcaataa tttgctttag cgtttcttca tcgaaaaact
     2281 tggctatggg agatttaaca ccttccgacg tgatgtttat ccatgccagc ccttttgctc
     2341 cgaattcttt tgatttttcc accagttcat caatttgcct gcgactgaag ctttcattgc
     2401 cttttttaat atttatccct tttacacaac cgccaccctt gaccgcctgg ctaaacactt
     2461 taaaatcggc attctctacc aaatccgaaa catcaaccat ctccaagcca aaccttgtat
     2521 ccggcttgtc ggaaccgtat ttttccatgg catctttgta ggtcattctg ggaaatggta
     2581 tttctatttg ttcgccggta actctttcaa ctacatattt tataagctgt tcgtttatgg
     2641 agatgacatc ctcagcatct acaaaagaca tttctatgtc aagctgtgtg aactcaggct
     2701 gcctgtcggc tcttaagtct tcgtccctaa agcaccgagc tatttggtag tatctctcca
     2761 ttcccgatac catcagaatc tgtttgaata gttggggtga ttggggcagt gcataaaact
     2821 gtccaggatt tagcctgctt ggaacaagga agtctctagc gccttcaggg gtactctttg
     2881 tcagaatagg cgtctctatc tctagaaagc ccttttcgtc aagaaaatct cttatggctt
     2941 tttttacttt gctgcgaaaa atcatattgt gctgcatttt aggccttctc aaatcaagat
     3001 acctgtactt aagccttata ctttcatcaa ctttaacatc atcttcaatg gcaaagggag
     3061 gggttttgga agcactcaaa attttcactt cttctcctat tacctcaata tatccggttg
     3121 aaatcttagg atttacattc tcttccgggc gtttaacgac cttccccttt accgctatga
     3181 catactcggt tctgagctta tctgctgttt caaaagcttc cttttgctct ggattaaaaa
     3241 ccacttgaac tacaccgctt cggtccctta aatcaacaaa gatgaggccc cccaaatctc
     3301 gccgtgtttg aacccaaccg gcaaggcata cttccttgcc ttcatgttct ggtctcaaat
     3361 taccgcagta aaaatttcgc ttgatattca tttgcatgtc aaacaactcc tttatgctta
     3421 acttttggtc tggattaata taaataaaaa aactctctca ctgatacaaa ttctatcagg
     3481 gacgagagtt ttcccgcggt gccaccctgt ttggataatc ccacttaaac ttttaacgca
     3541 agaaacgtaa acctctactt aagttcgaga tttcagctcc ggggtgttaa ttagcatatt
     3601 ccttttcaaa aagagctctc agtcacggcc ctctctccct gttgaaagca aatatgccct
     3661 tgccccatcc ttgcctttac tatatctttt ttgactatta taattaaatc agctccctgt
     3721 gtcaatgtac cggagtgttg a
//
LOCUS       DAONUO010000202         3723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17257_length_3723_cov_4.267176, whole genome shotgun sequence.
ACCESSION   DAONUO010000202 DAONUO010000000
VERSION     DAONUO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17257_length_3723_cov_4.267176"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PK768_07575"
     CDS             complement(<1..328)
                     /locus_tag="PK768_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HQA60868.1"
                     /translation="MKHVMSVSIGSSKRNHRVEVEVLGERFIIERIGTDGDIKKAIEI
                     IKEYDGKVSAFGMGGIDLYISVGDKRYTIKDAVPIARAAKLSPIVDGSGLKNTLERKV
                     IDYLIRE"
     gene            complement(330..863)
                     /locus_tag="PK768_07580"
     CDS             complement(330..863)
                     /locus_tag="PK768_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQA60869.1"
                     /translation="MDLVRIGDKLISFSRIVTTIKEMLDLRQQGLSQAEVAKRFNTDR
                     TFLSRLEGLGEIRKGKTIALIGFPIKNIEELKQVAVEEGVDYSLLMTDRERWDFVYEK
                     SGIELLNEVMSIIYKVRQYDVVIMLGSEQRLRLFKALLDNEVVSIEIGKSPITQDVYV
                     EPDNLRNIIKTLKKADE"
     gene            1016..2146
                     /locus_tag="PK768_07585"
     CDS             1016..2146
                     /locus_tag="PK768_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA60870.1"
                     /translation="MKVISGAIVGLGGFARIIAAAIRESRRVSLIAGCDIVPERLNEF
                     RKEAGMERLYDSIDKLLADPEIDFVIIATLPAQHYDMGRLALEAGKHVFFEKPGALMP
                     QQLAKLIELAESKGLKTSIDFVMRRNPLYLILKEICSLNIFGLLERAALENYAHDDSL
                     PPEHWFWDYEKSGGIWVEHGVHFFDLVNWLMGMPKRGNGDKIPRGNGLIDRVLGTAYH
                     DKGAVISYYHGFTKPEAFEKTLFSFVFERAYANIEGWIPISLSIDAMVTAEIEKLLMA
                     DLLMKARSFLPGVDLSLETKLLNEWDGEKTFKGRGKSYGATARAEFTYRLSCDRWEVY
                     RACVRQGIEDLAAAITAEKESPDVTLYDAKKALDVACMMEGR"
     gene            complement(2174..3133)
                     /locus_tag="PK768_07590"
     CDS             complement(2174..3133)
                     /locus_tag="PK768_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60871.1"
                     /translation="MEVYAIPIINGVLPRTDGGVLQGVFLEPFFANMLAKTGVGGDIF
                     LFPLSTDDQRTYPVGVLARIEDLWKDKIPQTGKSALFARVSGRDRCKAKSFAVSKEGL
                     LALDLEKIDIHELRSSGYPVICGAGWYPTGGYTSFGSDRRDVEITIYGFDLETGKDVA
                     IVGHLGKEIEPEKAHTVEHAIIRSLKNYAMCTPKTLRECMERETEELKWSVEIGIAKK
                     LPEMFGVTRSGFCGNPLTQMASFYLSEELKKQITSGANIIDSLNTARNKTVSKLTKEM
                     DISTQKGVRHLQGLKKGMFHDDTPEEMSVIKKVIQKFPANPWS"
     gene            complement(3148..>3723)
                     /locus_tag="PK768_07595"
     CDS             complement(3148..>3723)
                     /locus_tag="PK768_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HQA60872.1"
                     /translation="PVTVKIRKGWDEESVNAVEFSLMAENSGATMIAIHGRTREIFYR
                     GQADWDIIRDVKRAVSIPVIGNGDVFTPEDAAGMIQMTNCDGIMIGRGALGRPWLFGE
                     IKHYLKTGRKLSPPSLDERYRTILLHLDKAISYHGERLGILEMRKHLAWYIKGLPHSA
                     PVKNALQQCKDADSIREILEEYFSRLVKLGC"
BASE COUNT          919 a          916 c          840 g         1048 t
ORIGIN      
        1 tttcccttat aagataatct ataactttcc tctccaaagt attcttaaga ccagaaccgt
       61 caacaatagg ggaaagcttg gctgccctgg caatcggcac ggcatctttt atcgtgtagc
      121 gcttgtcacc aacggatatg taaaggtcta tcccgcccat gccaaaggcg ctgactttgc
      181 cgtcatattc ttttataatt tcgatggctt tttttatgtc gccatcggtg cctatgcgct
      241 cgattataaa ccgttctccc aggacctcga cttctaccct gtggtttcgc ttagaggagc
      301 ctatgctcac gctcatcaca tgtttcattt cactcatccg ccttctttaa agtcttaata
      361 atattcctca agttgtccgg ttcaacgtaa acgtcctgtg ttatgggcga tttgcctatc
      421 tcaattgaaa caacttcatt gtccagcagc gccttaaaca gcctcagcct ttgttccgag
      481 cccagcatta taaccacgtc gtactgcctc accttgtaga tgatgctcat tacctcattc
      541 aagagctcga tgccgctttt ctcgtacacg aaatcccagc gctcccggtc ggtcatcagc
      601 agggagtaat ccaccccttc ttctacagcc acttgcttta attcctctat gttctttatc
      661 ggaaagccta tcaaagctat agttttgccc tttcttatct ctccgaggcc ttccaaacgt
      721 gacaaaaagg tcctgtcggt gttgaacctt ttggccactt cggcctggga aagtccctgc
      781 tgtctcagat ccagcatctc ttttatagtc gtcactatgc gggaaaagct tatgagttta
      841 tctcctattc ttacaaggtc caccacaaca cctccatgtg cacatttttg tgctcatctt
      901 attttatcct acagaaacta aaaaatcaag attttattaa gtctttgtgg ggtctacaat
      961 gaaattattt tttgccaaag ggggaatatt aatagcaaaa aaggcggagg tttttatgaa
     1021 agtaataagc ggagctatcg tcggcctcgg aggctttgca aggatcatag cagctgccat
     1081 aagggaaagt agaagggttt ccttgattgc cggctgcgat atagttcctg aaaggctaaa
     1141 tgagttcaga aaagaagcgg gtatggaaag gttatatgat agtatagata aattattggc
     1201 agacccggaa atagattttg tcataatcgc gaccttgccg gcccagcatt atgatatggg
     1261 aaggctggcc ttagaagccg gcaagcacgt attttttgaa aagcctgggg cccttatgcc
     1321 ccagcagctt gcaaaactca tagagcttgc cgaatcgaaa ggtttaaaaa cgagcatcga
     1381 ctttgtgatg cggagaaatc ccctgtactt aatactaaag gaaatctgca gtttaaatat
     1441 tttcggtctc cttgaaaggg ctgccttgga aaactacgcc cacgatgata gcctgccgcc
     1501 ggagcactgg ttctgggact acgaaaaaag tgggggcatc tgggtagaac atggagttca
     1561 ctttttcgat ttggtcaatt ggctgatggg gatgccgaag agaggcaatg gagataagat
     1621 tcccagagga aatggcctca tagacagggt gctgggcaca gcttatcacg acaaaggtgc
     1681 agttattagc tactaccacg gctttacaaa gcctgaggcc tttgaaaaaa cgctcttttc
     1741 ctttgttttt gaaagggcct atgccaatat tgaaggctgg atcccgataa gtctttccat
     1801 agatgccatg gtgacagctg aaattgaaaa attgctaatg gcggatttac tgatgaaagc
     1861 ccgctccttt ttgccgggag ttgatctaag tctagaaacg aaactattaa acgagtggga
     1921 tggtgaaaaa actttcaagg gcagggggaa aagttacgga gctacggcca gggctgaatt
     1981 tacttataga ctcagttgcg accgctggga agtgtatagg gcctgtgttc ggcagggtat
     2041 tgaagacctt gccgccgcca taaccgctga gaaggaatcg cccgatgtga cgctgtatga
     2101 tgccaaaaaa gcactggatg ttgcctgcat gatggaggga cgataaagtg cggccaaagg
     2161 ccgcactaga attttaactc cagggatttg caggaaactt ttgaattacc ttctttatta
     2221 cactcatctc ttcaggcgtg tcatcatgga acatgccctt ttttaagccc tgaaggtgtc
     2281 tcactccctt ttgagtgctt atgtccatct cctttgtcag ttttgaaacc gtcttattcc
     2341 tggcggtgtt cagcgaatct ataatattcg caccgctcgt aatttgtttt ttgagttctt
     2401 cagacaggta aaaggaagcc atctgtgtca gcggatttcc gcagaaaccg cttcgggtca
     2461 cgccgaacat ttcgggaagc tttttggcaa tcccaatctc cacagaccac ttgagttcct
     2521 cggtctccct ttccatgcat tcccttagag tcttgggagt gcacatggcg tagtttttaa
     2581 gggagcggat aatggcatgt tcgactgtat gagccttttc cggctctatt tccttgccca
     2641 ggtggccgac gatggccaca tccttgcccg tttccaggtc aaaaccgtat atcgtaatct
     2701 ccacgtccct cctgtcagaa ccaaagctgg tgtaccctcc cgtaggatac cagccggcgc
     2761 cgcagattac tgggtatccc gatgagcgca gttcgtggat atctattttt tccaggtcca
     2821 gggccagcag cccttccttg gaaacggcaa agctcttggc cttgcatcta tctctacccg
     2881 acacccgggc aaatagggca cttttgcccg tttggggaat cttatccttc cacaggtcct
     2941 cgattctggc caggacacca acgggataag tcctttggtc atcagttgac aggggaaata
     3001 gaaagatatc gccacctaca ccggtctttg ccagcatatt ggcaaagaag ggttcaagaa
     3061 acacgccttg gagcacacca ccgtccgtcc gtggaagcac tccgtttata ataggtattg
     3121 cataaacttc caaggcatac acctccttta gcagccaagc ttgacaagcc ttgaaaagta
     3181 ctcttccagt atttctctta tgctgtctgc atccttgcac tgctgcagtg catttttcac
     3241 aggagccgaa tggggcaatc cttttatata ccaggccaga tgcttacgca tttctaaaat
     3301 ccccaaacgc tctccatgat aagatatggc cttatctaag tgaagtaaaa ttgtcctgta
     3361 tctttcatct aaggagggtg gggagagttt ccttcccgtc tttaaatagt gtttgatctc
     3421 cccgaaaagc cagggcctgc ccagggcccc tctgcctatc atgatgccgt cacagttggt
     3481 catttggatc ataccggcag catcttcagg cgtaaagaca tcgccgttgc cgattacagg
     3541 gatactgacg gctctcttta catctcttat tatatcccaa tcggcctggc cccggtaaaa
     3601 gatttccctg gtcctgccgt gaattgcaat catggtcgcg ccgctgtttt ccgccatgag
     3661 ggaaaactcc accgcattta cgctttcttc atcccagcct tttctgattt ttactgtcac
     3721 ggg
//
LOCUS       DAONUO010000203         3694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17429_length_3694_cov_3.974718, whole genome shotgun sequence.
ACCESSION   DAONUO010000203 DAONUO010000000
VERSION     DAONUO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17429_length_3694_cov_3.974718"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK768_07600"
     CDS             <1..602
                     /locus_tag="PK768_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA60873.1"
                     /translation="KKSLNGIRNTGLSVWLVAAIWMMYNASVISYLTFGGDYYVSVGY
                     SASYAGFLTSLLMIGSLLLSPLVGYLIDKVGKQEYIIAGSSVVLAALMFFVPRTELNP
                     LLLGILIGIASPFVPAPVFSLVPKLLPPEKLGLGYGILSTCLNVGVLIGPLMVGLSYD
                     RTLSYLSGFNLMAIFALSTAIIAILFPFINKSAQIKEES"
     gene            693..1424
                     /locus_tag="PK768_07605"
     CDS             693..1424
                     /locus_tag="PK768_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HQA60874.1"
                     /translation="MESIGREFIERTKYKYLGESDQFRNLPQPPLELEAESNKKILAL
                     PNPKALEVNVKLVDAMENRRSHRRYSDEPLTLEELSFLLWCTQGVKRISHNVTFRMVP
                     SAGARHAFETYILVNKVNGLSPGLYRYLALEHKLQEVEIGDEIADKLAYACLGQTFIK
                     ASAVTFIWLAVPYRMTWRYGERGYRYMLLDAGHVAQNLYLAAEAIDCGSCVIGAYDDD
                     AVNQLLGIDGKEQFAIYLATSGKKN"
     gene            1502..2800
                     /locus_tag="PK768_07610"
     CDS             1502..2800
                     /locus_tag="PK768_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQA60875.1"
                     /translation="MKSGDDMEGISYLLEGTKEFFIQNGAWGLFILSFAEASFFPIPP
                     DFVLLPLALISPGKALYYAAITSIASTLGGIFGYLIGKRAGRPILSRFIKEENFHKIE
                     DMFARYGGWAVAVAGFTPIPYKVFTIASGVFRMNFSIFFIATVLSRSARFFLEGVFIL
                     VLGDKALSYIDRLLGPGSFMLLGVVALLYFLIQKSGVSISFSLREGTLAHRLKGKLKS
                     FYGEFSIYLIAGLSIAVTFGILFFKLATEVLEREMEWFDTGIISIMTQSNFSFIDFIS
                     GTLNFLDNPIVFVILILLYLLYIKTFYRKNMYGGMAIVTFIGSFLLQWSFKSLYKRPR
                     LSSIDVDIFAYSFPSGFIVIFTALLGYVAFLLLKNKSKFKKVIIICAWLCIIIVVSAS
                     RIRAAISYPSDVLAGFLLGGLWLAVCIVGTKALEYYGIDS"
     gene            2890..3492
                     /locus_tag="PK768_07615"
     CDS             2890..3492
                     /locus_tag="PK768_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQA60876.1"
                     /translation="MARGFTDREREIIRNDLVATGRELFGTFGLKKTSIEDLTKAVGI
                     AQGSFYTFFNSKEELYLEVMDREGEAIKQKLLKEENIKDLTRERFKSFFKKVFEIVNS
                     NPIIRQMFFEEEVDTLIRKIPPEKMKEYHKKLMRDLLPIIKRWQDEGAIIRNYKPEVI
                     VALFQILYHPLLHKKDFDEDVFDEMLELLVDIVAKGLVVE"
BASE COUNT         1031 a          683 c          868 g         1112 t
ORIGIN      
        1 ttaaaaagag cctcaacggc attagaaata cgggactctc ggtctggctc gttgctgcaa
       61 tctggatgat gtacaatgcc tcggtcatat cctacctcac ctttggcggt gattattatg
      121 tatccgtagg ctatagtgca agctatgctg gttttttaac tagtctcctc atgattggtt
      181 cactgttgtt aagtcctcta gtgggatatt taatagataa agttggcaag caagagtata
      241 taatagccgg ctcaagtgtg gtactggcag ctttgatgtt ttttgtaccc cgcactgaac
      301 tcaatccctt gcttttgggg atattgatag gaattgcatc gccctttgtg ccggcacccg
      361 ttttttcctt ggtgcccaag ctgttacctc ccgaaaagct gggtctaggt tacggcatat
      421 tgtccacgtg tttaaacgtc ggagtcctta ttggaccact gatggttggc ctttcatacg
      481 atcggaccct aagctatctt tccggattta accttatggc catttttgct ctctctactg
      541 cgattatagc tattcttttt ccttttatta acaagtcggc gcaaattaag gaagaatctt
      601 aagctttatt aagagcatca tcctgatata tttgcatccg gattatctag cggagttttg
      661 atgaaaatat actttagaaa ggagaccata aaatggaaag tataggcagg gaatttattg
      721 agaggacaaa gtataagtac ctgggggagt cagatcagtt caggaatttg ccccaacctc
      781 ccctggagtt agaagctgaa agcaacaaaa aaatccttgc tctgccgaac cccaaagccc
      841 tggaagtgaa cgtaaaatta gttgatgcca tggaaaacag gcggtcacat cgaaggtact
      901 cggacgagcc gcttacatta gaagagctgt cctttttgct gtggtgcaca cagggggtaa
      961 agcgaatctc acataacgtc acattcagga tggttccatc ggcaggggcc cggcacgctt
     1021 ttgaaaccta cattttggtg aacaaggtaa acggtttaag cccggggcta tatagatacc
     1081 tggcacttga gcataaactg caggaagtgg aaatcggtga tgagatagca gataagctgg
     1141 catatgcctg tttgggccag actttcataa aagcttcggc tgtgaccttt atttggctgg
     1201 cggtgcccta tcggatgact tggcggtacg gtgagagagg atacaggtac atgctcttgg
     1261 atgcaggcca cgtggcacag aatctgtatc tcgccgcaga agctatagat tgcggcagct
     1321 gtgttatagg cgcctacgat gatgacgctg taaatcagct gctaggcatt gacggaaagg
     1381 aacaattcgc aatatatttg gccacttcgg gtaaaaaaaa ttaatattcg gcatcattgc
     1441 cgattttttt atttctgata ttcatttttt tcagctaatc gtataaactt aaggaagtag
     1501 cgtgaaaagt ggtgatgata tggaaggtat ttcttatctt ttagaaggga caaaggagtt
     1561 tttcatacaa aatggtgcct ggggactatt catcttgtct tttgccgagg cctcattttt
     1621 tcctataccg ccggatttcg tccttttgcc tttggccctc atttcgcctg gcaaagcttt
     1681 gtactatgct gccataactt ctatcgcctc tactctcggt ggcatttttg gatacctcat
     1741 cggaaaaagg gcaggtaggc ccatactttc tcgatttatc aaagaagaaa atttccacaa
     1801 aattgaagat atgtttgccc gctacggcgg atgggctgta gctgtagccg gttttacccc
     1861 aataccctat aaggttttta ccattgcctc gggggtattt cgcatgaatt tttctatctt
     1921 ctttattgca acggtactca gcaggagtgc caggtttttc ctggaaggag ttttcatatt
     1981 agtgctgggg gacaaggccc tttcttacat agatagatta ttaggacccg gttcttttat
     2041 gttacttgga gtagtcgcat tgctgtattt tttgatacaa aagagcggtg tttctatcag
     2101 cttcagcttg agggaaggga ctttggccca ccgtttaaaa gggaaactca agtcctttta
     2161 cggggaattt agcatatact tgatagcggg attaagcatc gctgttactt tcggcatatt
     2221 attctttaaa ctggcaacgg aagtgctgga aagggaaatg gaatggttcg atacgggtat
     2281 aattagtatc atgacacaaa gcaattttag ctttattgat ttcatatcag gcaccttaaa
     2341 ttttctagac aatcctatag tgtttgtcat tttaatactg ctttacttgc tttatataaa
     2401 gacgttttac aggaaaaaca tgtacggcgg aatggccata gttacattta taggcagttt
     2461 tcttttgcaa tggagcttca agtccttgta taagaggcca aggctttcaa gtatagatgt
     2521 tgacatattt gcctacagtt ttcccagcgg ctttattgta atttttactg cccttttagg
     2581 ctatgtggct tttttgttat taaagaacaa gagcaagttt aaaaaagtaa ttatcatatg
     2641 cgcctggctg tgtatcataa ttgttgtaag tgcgagcagg attcgtgcag ctatcagcta
     2701 tcccagtgac gtgttggcag gctttttact gggaggatta tggctggcag tttgcatagt
     2761 cggcacaaag gccttggagt attacggtat agatagttag gctattgaca gaatagcttt
     2821 atatagggta aaattttatg aatgaaaata ttttttattc ataagctcaa agactataga
     2881 ggtgaaataa tggcgagggg atttacggac agagagagag aaattatcag aaatgacctt
     2941 gtagcaacgg gtcgggagct tttcggcact ttcggcctca aaaaaaccag tatagaagat
     3001 ttgaccaagg cggttggaat tgcccagggc tccttttaca ctttttttaa ttccaaggaa
     3061 gagctttatc tggaggttat ggacagggaa ggggaggcta ttaagcagaa gcttttaaag
     3121 gaagagaaca tcaaagatct gacccgcgag cgctttaaaa gtttttttaa gaaagtgttc
     3181 gagatagtca actcaaatcc aataattcgg cagatgtttt ttgaagagga ggtagatact
     3241 ctaatcagga agattccccc ggaaaaaatg aaggagtacc ataaaaagct catgcgcgac
     3301 ttgttgccga taataaaaag atggcaggat gaaggggcca tcataaggaa ttataagcct
     3361 gaagtaattg tggcattgtt tcagatccta tatcatccat tactgcacaa aaaagacttt
     3421 gatgaagatg ttttcgatga aatgctagag ctcttagtgg acatagttgc aaagggacta
     3481 gttgttgaat aagactagac ttgatttcat aagaaaactt gccatttaga aatctatgaa
     3541 atgaggagtg taccatgagg aagaaagcat acataactgc ccttatttta atagctgcga
     3601 tggtcgtttc tggctgcggc gcacaaactt caagcgaaac ggagcaagaa aagatagttc
     3661 ccgtcaaggt gacactatct caaaaagagg attt
//
LOCUS       DAONUO010000204         3681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17498_length_3681_cov_3.753447, whole genome shotgun sequence.
ACCESSION   DAONUO010000204 DAONUO010000000
VERSION     DAONUO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17498_length_3681_cov_3.753447"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PK768_07620"
     CDS             complement(<1..326)
                     /locus_tag="PK768_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60877.1"
                     /translation="MITKAQLLCKNIVLKVVQSMKRFPEAIFFYCATTLILIYMNHIA
                     YNKATFDFLTRIAMILALGAVVFLCIRTFLERKHPVAIKTTIAIYASAIIGLFIYFFL
                     LLDELNT"
     gene            498..1079
                     /locus_tag="PK768_07625"
     CDS             498..1079
                     /locus_tag="PK768_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HQA60878.1"
                     /translation="MGVTYPTRAEAFELLKKYNSNESLIRHALAVEAVMRHFAELFGE
                     PDVEKWAIIGLVHDLDYEMYPDQHCQKVREILMDEGWPEDYIRAIQSHGWGICSDVEP
                     VEKMEKVLYTIDELTGLIAATALLRPSKSILDLKAKSVKKKWNQKSFAAGVNREIIEK
                     GLELLGMERDFIIEETIKGMQKVAEEIGLKGNL"
     gene            complement(1161..2087)
                     /locus_tag="PK768_07630"
     CDS             complement(1161..2087)
                     /locus_tag="PK768_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQA60879.1"
                     /translation="MSNGDLKLAYFFALLHTLITGLSFLFVKTALKFASPLDVLAFRF
                     SASLLALMVPIMLKKIHLNYAGKNLLQLCSLGLLYPIAFFFFQTVGLVFATSSEGGII
                     QSVTPIFTMILAAIFLKERTNNYQKIAILMSVAGVVYIFVMKGAGLDLSEVLGITLLL
                     ISALASAGYHILTRITTRQFSITEISFMMTLLGFVFFNAAAIIGHMARGSLDQFLTPL
                     QNLEFVVSILYLGILSSLVTSLLNNYILKRIEAAKVSVFGNLRTVVTIFAGVYFLKEQ
                     LFAYHIIGSLMIILGVLGTNYAGKLPQLSRKT"
     gene            complement(2149..2979)
                     /locus_tag="PK768_07635"
     CDS             complement(2149..2979)
                     /locus_tag="PK768_07635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HQA60880.1"
                     /translation="MNGYKLIVLDLDGTLLDSNKEITDKDRKAIDLLKNMGILVTIAT
                     GRHPSNSSKYVYELGITTPIIACNGALIRDVRNGTTLRMKEVDSDVALKVINYCKQKQ
                     LDFLVYTSDAIYYSDNSERIKAIQAYNDSVSQELRVPLYRMKSLDISQQSIIKILVRS
                     NEYQVFEKLEEHVKKDGRLTIVKSESDLIDIMTKGVSKGDGLEFLAKHLDIDLRYTVA
                     FGDNHNDMSMLEVAGLSIAMGNAEEELKEIADYITVSNDESGVSHAVYKYILKGNGEV
                     "
     gene            complement(3099..>3681)
                     /locus_tag="PK768_07640"
     CDS             complement(3099..>3681)
                     /locus_tag="PK768_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF342 domain-containing protein"
                     /protein_id="HQA60881.1"
                     /translation="EKFGYLLDAAKELCERTETTFHEMPFEGMQEFIYRVKKDLINFS
                     LAIYDLAKFEKLEEQARTLVQNLAFYPATDSNIVASSIQNSVVFATGDIDVVGSGCYI
                     SKIQAGRKVNIKAVVRGGEVIAGSDVFIGELGSIGGSPTRVVAEHDAVVKIGYAHVNS
                     VVVIGGQSYRFSRDEENAKFWLDGDGNLRSSNY"
BASE COUNT         1144 a          760 c          690 g         1087 t
ORIGIN      
        1 gtgtttaatt catctaacaa gagaaaaaag tatataaaga ggcctataat ggcactcgca
       61 tagattgcaa tcgttgtttt gattgccaca gggtgctttc tctccaaaaa tgtcctgata
      121 cataagaaca ccacagctcc aagggccaag atcattgcaa ttctcgttag aaaatcgaag
      181 gttgctttgt tataagctat atggttcatg tatatcaata ttaaagtggt ggcacagtag
      241 aaaaatatgg cttcaggaaa gcgcttcatg ctttggacaa ctttcaacac tatgttttta
      301 caaagcagct gagcttttgt tatcattttt tacctccata ttataatctt cctacatcta
      361 ttcgaaatcc aatacagttt ttccttccta ttattttaat aaatttaaat tcaggcaaaa
      421 accccgaaag cttgacacat tgtgggcaaa agtttactat ttataataag cattatatta
      481 gtgagggatg gattaatatg ggcgtaacat atccaacaag ggcagaggct tttgagctgc
      541 taaaaaaata taacagcaat gagagtctga ttcgccatgc attggctgtt gaagcagtaa
      601 tgcgtcactt cgctgagctt tttggcgaac cggatgtgga aaaatgggca ataataggtc
      661 ttgttcatga cctggattac gaaatgtatc cagaccaaca ctgccaaaaa gttcgggaaa
      721 ttttaatgga tgagggctgg ccggaagact acataagggc gatacagagc cacggctggg
      781 gaatttgttc cgatgttgag cccgttgaaa aaatggaaaa ggtattatac accattgatg
      841 agttgacggg ccttattgcg gcaactgctt tacttcgccc cagcaaaagc atattggacc
      901 ttaaagctaa gtctgttaag aaaaagtgga accaaaagag ctttgccgca ggagtcaaca
      961 gggaaatcat cgaaaagggt ttagagctct tgggaatgga gcgagatttt ataattgaag
     1021 aaaccataaa gggaatgcaa aaggttgccg aagaaatcgg cttaaaggga aatttataat
     1081 tgagtattaa cacatgataa acaacaaaaa gaggcttttt aaggatatta aagcctctat
     1141 atttatttat acaatactgt tcatgttttc ctcgataatt gcggcagttt gccggcatag
     1201 tttgtgccca gcacccctaa gatgatcatc agcgaaccaa taatgtggta ggcaaagagc
     1261 tgttctttaa gaaaatatac cccggcaaaa atagtgacca cggttcttag attgccaaag
     1321 acacttacct ttgcagcctc tatccttttg agtatataat tgttcaaaag ggatgtaact
     1381 aatgatgaca aaattccaag atataggatg gaaaccacaa attcaaggtt ttgtagggga
     1441 gttaagaatt gatcaaggct cccccttgcc atgtgcccta ttattgcagc ggcattaaaa
     1501 aatacaaatc caagtaatgt catcataaag ctaatttcag ttattgaaaa ctgccgtgtc
     1561 gttatacggg taagtatgtg ataaccagca gaagccaaag cagaaatcag taatagggta
     1621 atacctagca cttccgataa atccaaacct gctcctttca taacaaagat gtaaaccacc
     1681 cctgcaactg acattaaaat cgcaatcttc tgatagttgt tggtcctttc ctttaaaaaa
     1741 atagcggcta aaatcatcgt aaaaatcggt gtaaccgatt gtataatccc tccttcagag
     1801 gaagttgcga aaactaggcc aacggtttgg aaaaagaaaa atgctatagg ataaagcagc
     1861 cctaatgagc ataattgaag caggttcttg ccggcgtaat taagatgtat ttttttcaac
     1921 ataatgggca ccatcagcgc taatagagag gcagaaaatc gaaaggcaag gacatctaag
     1981 ggacttgcaa actttagtgc agtctttacg aataaaaatg ataggcctgt tatcaaagtg
     2041 tgcagcaggg caaaaaagta agcaagcttt aaatcgccat tgctcacgga aggcacctcc
     2101 taatttatat tcccctcata cagctgcata aaatatcttt ttcaaaaact agacctcacc
     2161 attccctttt aaaatatact tgtaaactgc atggcttact cccgattcat cattggatac
     2221 agtaatgtag tcggcaatct cttttagctc ttcctcggca ttgcccatgg caattgaaag
     2281 gccggcaact tctaacatac tcatatcatt gtgattatcg ccaaaggcaa ccgtatacct
     2341 gaggtctata tcaagatgct tagccaaaaa ctcaaggcca tccccctttg atacgccttt
     2401 agtcattata tcaataagat cactttcgga ctttacaatg gtcaacctgc catccttctt
     2461 tacatgttcc tctaactttt caaagacttg atattcatta cttctcacca atatcttaat
     2521 aatgctctgc tgcgaaatat ccaagctttt cattctatag aggggaacgc gcagctcctg
     2581 gcttacgctg tcgttatagg cttgaatggc ctttatccgt tcggaattat cgctgtaata
     2641 aatggcatca gatgtgtaca ctaaaaaatc tagttgtttt tgtttgcagt agtttataac
     2701 ttttagtgcc acatcagaat caacttcctt catgcgcaaa gtggtgccgt ttcgaacatc
     2761 cctaatcaaa gcaccattac acgctatgat gggagtggta atccccagct catatacata
     2821 tttgctggaa tttgaaggat gccttcccgt agctattgtt actaatatac ccatgttttt
     2881 caataaatct attgcttttc gatctttatc agttatttct ttgttagaat ctagcaaagt
     2941 tccgtctaaa tccaatacaa tcaatttata tccattcact tctttatgct ccaccctata
     3001 gataatttca agcttattat accataaata cgaaaaaatg cggtcagaaa aagcaccaac
     3061 cgttcttggg ttggtgcttt ggtttgtcgc agggtatttt aataatttga acttctcaag
     3121 ttgccatcac catccagcca aaactttgca ttctcttcat ccctactaaa gcggtaagat
     3181 tgacctccta taactaccac agaatttaca tgtgcgtatc caattttaac cacagcatcg
     3241 tgttctgcca cgactctcgt tggggagcct cctattgagc cgagttctcc gatgaagaca
     3301 tcgctgccgg cgattacttc accacctcga actacagctt tgatgttcac ttttcgtcct
     3361 gcctggatct tagagatata gcagccggat cctacaacat ctatatcacc tgtggcaaaa
     3421 actacagaat tttgtatgct cgatgccact atattactgt ctgtagcagg gtaaaaggct
     3481 aagttctgca ctaaggtcct tgcctgctct tcaagttttt caaatttagc taaatcgtat
     3541 attgccagag aaaagttaat caggtccttt ttaaccctgt atataaactc ctgcatccct
     3601 tcaaaaggca tttcatgaaa cgtggtttct gtgcgctcac acagttcttt tgcagcgtca
     3661 agaaggtaac cgaatttttc c
//
LOCUS       DAONUO010000205         3668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17578_length_3668_cov_3.701910, whole genome shotgun sequence.
ACCESSION   DAONUO010000205 DAONUO010000000
VERSION     DAONUO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17578_length_3668_cov_3.701910"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..355
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..26
                     /rpt_unit_seq="ttagcttacctataagggattgaaac"
     gene            512..682
                     /locus_tag="PK768_07645"
     CDS             512..682
                     /locus_tag="PK768_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60882.1"
                     /translation="MASLTSLTVGHSRKRPGASSAFRDRAVSFAPMAPAALMKKEICS
                     EVFVVSMQMPWL"
     gene            722..1201
                     /locus_tag="PK768_07650"
     CDS             722..1201
                     /locus_tag="PK768_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60883.1"
                     /translation="MHIMRIRPLNHALEIAGLCSMEALDIAGEGLREVCRGLNIPPVL
                     SFGTCTDTGRISMLVTALADHLDLDIPDLPIAISAPEWMEQKATIDGVFALAYGTLTH
                     ISPAPFISGAGRLVKLLTEDSEEITGGKVLLGDEPKEVADKIESHILEKWKALGMKY"
     gene            1322..1585
                     /gene="cas2"
                     /locus_tag="PK768_07655"
     CDS             1322..1585
                     /gene="cas2"
                     /locus_tag="PK768_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HQA60884.1"
                     /translation="MFVILVYDVGEKRVAKVLKICRKYLTWVQNSVLEGEISAANLKK
                     LKLELKGAIDEEEDSVIIYILRSTRYSERQTMGIKKGGDELFI"
     gene            1600..2235
                     /locus_tag="PK768_07660"
     CDS             1600..2235
                     /locus_tag="PK768_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HQA60885.1"
                     /translation="MSVKSKFYEARCQKAVKALIKNGFDAIYVPTKEEAVKKALDLVP
                     PNAKVGVGGSVTIRELGIVDELKARGHELFDHYLADTPDEKNRMRKGQLTCDVFFSST
                     NALTLDGKLVNIDGTGNRVASMIFGPGHVVLIVGANKITDDLQQAMIRAKHLAAPMNA
                     MRLNLKTPCATTGYCSDCNSEDRICKVTTIIEYKPDLTNFTVILVGEEIGY"
     gene            complement(2316..>3668)
                     /locus_tag="PK768_07665"
     CDS             complement(2316..>3668)
                     /locus_tag="PK768_07665"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQA60886.1"
                     /translation="SQKDLKKVFELADKYIDFMSRCKTERECVEEFRNRAERAGYKNL
                     QDLIEANKKLKAGDKVYAELMGKTIALFVIGKRPLEDGMLILGAHVDSPRLDLKQNPL
                     YEDTGLALLDTHYYGGIKKYQWVTMPLALHGVIVKKDGTKINVVIGEDDGDPVFGVSD
                     LLIHLAKDQMKKSLAEGVEGEKLNVLVGSIPINDKKVKNRVKQNILKILNDKYGIVEE
                     DFVSAEIEVVPAGRARHYGFDNSMIMAYGQDDRVCAYTSFEAMMEIEKPEKTCVTLLV
                     DKEEIGSVGATGMHSRFFENTVAEIANLMGNYSELTIRRALANSKMISSDVSAAFDPN
                     YPEVMEKQSAAFFGKGIVFNKYTGARGKSGSNDANPEFIAEIRKIMEKHDVTWQISEL
                     GKVDQGGGGTIAYILANYGMEVIDAGVALLNMHAPWEISSKADLYEAKKAYYAFLVEA
                     "
BASE COUNT          998 a          807 c          833 g         1030 t
ORIGIN      
        1 ttagcttacc tataagggat tgaaacatgt gtggagaaaa gataccattt cgctggaatc
       61 ttcgttttta gcttacctat aagggattga aactcataac ctattcccat ccaatcaatt
      121 tcaataagtt tttagcttac ctataaggga ttgaaactcg gtatcataaa gtctaccaat
      181 tagttcttcc aaggttttta gcttacctat aagggattga aacgctgttt tgaatcgaca
      241 gtcacactac ctatttcctt gtttttagct tacctataag ggattgaaac aaatgctcat
      301 ggtaagggac gccatcactc aggaagtttt tagcttacct ataagggaat gaaacgtctt
      361 tggaatttgt cttgcgttta aaatatctat tgtataaaac ctgctttaag gtaacttcct
      421 cgatctgcag tatataaaac catctttatg accgaaggcc gtttaagcta tcacgattcg
      481 gtcgaggaaa tgttaaagcg gattcgagaa gatggcctca ctaacgtctt tgaccgttgg
      541 gcacagcagg aaaaggccag gtgcaagttc tgccttcagg gatcgagctg tcagctttgc
      601 tcccatggcc cctgccgcat taatgaaaaa agagatatgc tccgaggtgt ttgtggtatc
      661 gatgcagatg ccatggctat aaggaagtta ctactgcaaa atgctatggg agcagccaca
      721 tatgcacatc atgcgtatca ggcctttaaa ccacgcttta gaaattgcag gtctttgcag
      781 catggaagct ctggatatag ccggtgaagg cttgagggag gtatgcagag ggctcaatat
      841 tcccccggtt ttaagctttg gaacctgcac tgacacagga aggatttcca tgctggtgac
      901 ggccttggca gatcacctgg acttggacat tccggacctt cccattgcca tttctgcccc
      961 tgagtggatg gagcaaaaag ctactataga cggagttttc gccctggcat atggcacatt
     1021 aactcacatt tcccccgctc cttttatatc cggagctgga agacttgtaa aactgctcac
     1081 tgaagactcg gaagaaatta caggtggcaa agtcttgctg ggagatgagc ctaaagaagt
     1141 ggcagataaa atagaaagtc atatactaga aaaatggaaa gcccttggaa tgaagtacta
     1201 agaaaagccc actagggctt tttcatttgc caagtataga ggaaattaga gtttgattat
     1261 agaataaaac ataaagcaaa tattacaaaa cagtagttct gttgtaaatc aggtggacaa
     1321 tatgtttgtt atacttgtgt atgatgtggg cgaaaagcga gtggctaaag tattaaagat
     1381 atgtcgaaag tatttaactt gggtccaaaa ctctgtcctt gaaggagaaa tttctgctgc
     1441 aaatttgaaa aagcttaagc ttgaattaaa aggggctatc gacgaagaag aggattcggt
     1501 tattatctac atactgagaa gtaccaggta ttcggagcgg caaacgatgg ggattaaaaa
     1561 gggtggagat gaattattta tatagatagg agggtttata tgagcgttaa gtcaaaattt
     1621 tatgaggcaa ggtgccaaaa agctgtcaag gctctcatca aaaacggttt tgacgccata
     1681 tatgtgccca ccaaggaaga ggctgtgaaa aaagcattgg acctagttcc cccaaatgca
     1741 aaggtaggtg tgggaggctc ggttaccatc cgtgaactcg gtatcgtgga tgagttaaag
     1801 gccaggggcc atgagctttt tgaccattat ctagcggata ccccggatga aaaaaaccgc
     1861 atgcggaagg gccagctgac ctgcgatgtc ttcttttcca gcaccaatgc cctaactctt
     1921 gacggcaagc tggtaaacat agacggcacg ggcaaccggg tagcatctat gatattcggg
     1981 ccgggacatg tggtcctaat tgttggcgcc aacaaaataa ctgatgacct gcagcaggcc
     2041 atgataaggg caaaacatct ggcagccccc atgaatgcca tgagactaaa cttgaagacc
     2101 ccctgtgcga caacaggtta ctgcagcgat tgtaactcgg aagacaggat atgcaaggta
     2161 accaccataa tcgaatacaa acccgatctg actaatttta cggtcatatt agtaggtgag
     2221 gagataggat actagatatt ttcataaaaa gtgttagaaa aacaaaaaag cctgtaccgg
     2281 cgggatgttg cccggtacag gccttttttt aatatctagg cctcaactaa aaaggcgtaa
     2341 tatgcttttt tggcttcata taaatcggct ttgctggaga tttcccaagg tgcgtgcatg
     2401 ttaaggaggg ctacgcctgc atcgatgact tccatgccat agttggccag gatgtaggct
     2461 atggtgccac caccgccctg gtcaaccttg cccagttccg atatctgcca ggtgacatcg
     2521 tgcttttcca tgattttccg aatttctgcg ataaactcgg gatttgcatc attgctgccg
     2581 ctcttgcccc tggcccccgt gtacttgtta aacacgatac ccttgccgaa aaatgcggca
     2641 ctttgctttt ccataacttc aggatagttg gggtcaaagg cggcactcac atcagaggat
     2701 atcattttgg aattggccag agcccgcctt atagtaagct cgctgtaatt ccccatcaaa
     2761 ttggcgattt cagctacggt gttttcgaag aaccttgagt gcatgcccgt cgcgccgaca
     2821 ctgccgattt cctctttgtc aacgaggaga gtgacgcatg tcttttccgg cttttcaatt
     2881 tccatcatgg cttcaaagga agtgtaagca cacaccctgt cgtcctggcc gtaagccatg
     2941 atcatgctgt tgtcaaagcc gtagtgcctc gcccttccgg cagggaccac ttcaatttct
     3001 gcggatacaa aatcctcttc gactataccg tatttgtcat ttaggatttt taatatattt
     3061 tgctttaccc tatttttgac cttcttgtca tttatgggaa tgctgccaac caggacattg
     3121 agtttttccc cttcaacacc ctcggccagg ctctttttca tctggtcttt tgctaggtga
     3181 atgagcaggt ccgatactcc aaacaccgga tcaccgtcat cttctccgat gactacattg
     3241 attttggtcc cgtctttctt tacaataacc ccgtgaaggg ccaggggcat tgtcacccac
     3301 tggtattttt taattccccc gtagtaatgg gtgtcaagga gggccagacc tgtatcttca
     3361 tacaggggat tttgctttag gtcaagcctg ggtgagtcta catgggcacc cagtatgagc
     3421 attccatcct ccaaaggcct ttttcctatc acaaacaggg ctatggtctt acccattaat
     3481 tccgcatata ccttgtcgcc ggctttcagc ttcttgtttg cctcaatcaa atcctggaga
     3541 ttcttgtaac cggctctttc agccctgttt cgaaattctt ccacacattc cctttcggtt
     3601 ttgcagcggg acatgaagtc aatatacttg tcagccaatt caaatacctt ttttaagtcc
     3661 ttttgtga
//
LOCUS       DAONUO010000206         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17618_length_3661_cov_2.872158, whole genome shotgun sequence.
ACCESSION   DAONUO010000206 DAONUO010000000
VERSION     DAONUO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17618_length_3661_cov_2.872158"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PK768_07670"
     CDS             complement(<1..432)
                     /locus_tag="PK768_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60887.1"
                     /translation="MADYREEILDVSSFPIEESERLVYAFNYKFPKKDAYMFLEGPSL
                     HIKLYQLDMTKYREFLDGLDISTELAEQVYDIIDSIGSYGINGNKRLFSGYNAERKVI
                     NRKAKEQSRSKHYYASDHNFSKVNNELPSAYENKIICADSLD"
     gene            789..1811
                     /gene="asrA"
                     /locus_tag="PK768_07675"
     CDS             789..1811
                     /gene="asrA"
                     /locus_tag="PK768_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HQA60888.1"
                     /translation="MGYRLSKDGLNAVLAEFSRHYKLYAPHRFPYQGEHSDTDSIRYA
                     EVKTVDEIELGEKSSHTFKEALIPISETLFFFTEDNIKESDPVHTGAIIFLRSCDLHA
                     VKRLDEIYLNNGADDYYYKRLRNNVKFVLLGCSSPFENCFCVDMGTNIINEYDASLDV
                     RDGQYYVDCKASDWNSLFQNHAEEILDVVPSHVDKTPTRVSLPDTNTIDTVTVIKSSM
                     WDEYDSRCIACGRCNCSCPTCTCFTMQDIFYTDNGRVGERRRVWASCMVDKFTDVAGG
                     GSYRNRYGERMRFKVLHKVVDFKQRFGYQMCVGCGRCDDVCPEYISFSHAVNKLSKLS
                     KEGRKQ"
     gene            1808..2602
                     /gene="asrB"
                     /locus_tag="PK768_07680"
     CDS             1808..2602
                     /gene="asrB"
                     /locus_tag="PK768_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HQA60889.1"
                     /translation="MSLNEYVPFLSKILSVQKHTDIEYTFRMAYEGSVNPGQFFEVSV
                     PKFGEAPISICGYGDGYVELTIRRVGKVTNEIFEHYEGDSLFLRGPYGNGFDLDEYRG
                     KELVVIAGGTGLAPVRGVIDYFASNPDQVKNLKVIAGFKSPSDILFFEDIARWKKTSQ
                     VILTVDCGEADFECHIGFVTEHISKLKFADESNAVAIVVGPPAMMRFSTLELLKMGFE
                     EENIWISLERRMCCGIGKCGHCKINDVYVCVDGPVFNYTKAKNLID"
     gene            2617..3600
                     /gene="asrC"
                     /locus_tag="PK768_07685"
     CDS             2617..3600
                     /gene="asrC"
                     /locus_tag="PK768_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HQA60890.1"
                     /translation="MDINTKNLKKNAFRVTKVRGYTASRVRIPGGHLNAEILEKVADI
                     ARKYGNGTVHITSRQGFEIPGIPFELIPKINEELQPIIDMLEINQPVRGEGYSASGTR
                     NIVACIGNRVCPYACYDTTAFAKRIEKAVFPHDLHFKIALTGCPNDCAKVRMHDFGIM
                     GMTMPVYDPSRCVSCGACEKYCRRKSVQAIEMVNERPVRNEEKCIGCGECVLNCPTRA
                     WTRGEGADKRYRLTLFGRTGKRNPRLGEDFIKWVDEDSIIKIILNTYDYVAKYIDKDA
                     PGGKEHIGYIVDRTGFEEYKKWALKGVNLPPGAVVYNPLYWKGIKYTEMTL"
BASE COUNT         1054 a          760 c          812 g         1035 t
ORIGIN      
        1 atccaggctg tctgcacaga tgatcttatt ttcatatgcg gaaggcaatt cattgtttac
       61 ttttgaaaaa ttatggtcac ttgcataata atgtttgctg cggctttgtt cctttgcctt
      121 tctgtttatc accttgcgct cggcattgta tccagaaaaa agtctcttgt ttccatttat
      181 cccgtagctg ccgatagaat caataatatc ataaacctgt tcggcaagtt ctgttgaaat
      241 atcaagtccg tccagaaact cgcgatattt tgtcatatcc agctggtaaa gcttaatatg
      301 tagtgacgga ccttctagaa acatataagc atctttcttg ggaaatttat agttaaaggc
      361 ataaacaagc ctttcacttt cttcaatggg aaaagatgat acatccaaaa tttcttcgcg
      421 ataatcagcc attttcgtca cccccaaaat tgtaggccat aattgcccaa ttgtcaaggt
      481 gtttgcctct accccgcggt ttgccttgac atcaatataa atcacttcgt gtttctgtaa
      541 tatcttaatt atatcacttc acgtcctagt cgtacacaaa aagtcaaaaa ggaatttttc
      601 tattcatgtc gaaattattt aacatcagct tatcgaccaa ttccttccac tgcttttcat
      661 ggtgttttat tcaatttacg attatagtta gcaatttgct gctcctttcg cagaaaaagc
      721 aaaagcttaa aacttatccc agatttctgc tgtttttgta tttttttaca acataaggag
      781 gtattttaat gggatacaga ctaagcaaag acggattaaa cgcagtcctt gccgaattta
      841 gccgtcatta caagctatat gcaccgcatc gctttccata tcagggcgag cattctgata
      901 ccgattccat tcgttatgca gaagtaaaaa cagtagacga aatagagcta ggcgaaaaaa
      961 gcagtcatac atttaaagaa gcgctaatac ctatttctga aacactgttt ttcttcacag
     1021 aggacaatat aaaggaatcc gatcctgtac ataccggagc tattattttt cttcgcagct
     1081 gtgatttaca cgcggtaaag cgccttgatg aaatttatct taataatggc gctgatgatt
     1141 attactataa acgcttaagg aataatgtca agtttgtgct tttaggctgt tcttctccat
     1201 ttgaaaattg tttttgtgtc gatatgggaa caaatatcat caatgaatac gacgcttcac
     1261 tagatgtccg ggatggccag tactacgttg actgcaaagc ttctgactgg aactctctat
     1321 ttcaaaacca tgcagaagaa atattggatg tggttccatc gcatgttgac aaaacaccca
     1381 ctcgtgtttc tttgcccgat actaatacta ttgacactgt cacagttata aaaagcagta
     1441 tgtgggatga atacgatagc cgttgcattg catgcggccg ctgtaattgt agctgtccta
     1501 cctgcacctg ctttacaatg caggacattt tttacacaga taacggcagg gttggcgaac
     1561 gtcgccgggt ttgggcatca tgcatggttg acaagttcac agatgtagca ggcggtggca
     1621 gctaccgcaa cagatacggt gagcggatgc gctttaaggt gctccataag gttgtagact
     1681 ttaaacagcg tttcggctat caaatgtgtg tcggatgtgg tcgctgcgat gatgtttgcc
     1741 ccgagtatat aagtttttcc cacgcagtaa acaaactgag taagctttcg aaggagggcc
     1801 gtaaacaatg agcttgaacg aatatgtgcc ttttttatca aaaattttgt ccgttcaaaa
     1861 gcacaccgat atcgaatata ccttccgcat ggcctatgaa ggcagcgtaa accccggaca
     1921 gttctttgaa gtttctgtgc cgaagttcgg cgaggcgcct atttcaatat gcggttatgg
     1981 agatggatac gtagaactaa ccattcgccg tgttggaaag gtaacaaacg agatatttga
     2041 acactatgaa ggcgacagcc tatttttgag aggcccttac ggcaatggat tcgaccttga
     2101 cgaataccgg ggaaaagagc ttgttgtgat tgcaggcggc acaggccttg cacctgtccg
     2161 tggggtcata gattattttg cttctaaccc cgatcaggtt aagaacctca aggtgattgc
     2221 gggttttaag tctccttccg atatactctt ttttgaagat atagcccgtt ggaaaaaaac
     2281 cagccaggtc atattgaccg ttgactgcgg cgaagccgat tttgagtgcc atatcggttt
     2341 tgtaaccgaa catataagta aattgaaatt tgctgatgaa agcaatgccg ttgccattgt
     2401 cgtcggcccg cctgctatga tgcggttttc cacattagag cttctaaaaa tgggctttga
     2461 agaagaaaat atttggattt cccttgagag aaggatgtgc tgtggcattg gaaagtgtgg
     2521 ccattgtaaa ataaatgatg tctatgtatg tgttgatggc cccgtattta actacaccaa
     2581 ggctaaaaat cttatcgact aaggagcgga ataaatatgg atataaatac aaagaattta
     2641 aaaaagaacg ccttccgcgt taccaaagtc cggggatata ccgcaagccg ggtgcggatt
     2701 ccgggtgggc atctaaatgc agaaatcctt gaaaaagtag ccgatattgc tagaaaatat
     2761 ggcaacggga cagttcatat cacatcaagg cagggttttg aaatacctgg tattcctttt
     2821 gaacttattc cgaagataaa cgaagagctg caacccatta ttgatatgct ggaaattaac
     2881 cagcccgtga ggggcgaggg gtattctgct tcgggcacac gaaatatcgt tgcctgtata
     2941 ggcaaccgcg tctgccctta tgcctgctat gataccacgg cttttgcaaa gcgtatagaa
     3001 aaggcggttt tcccccatga cctacatttt aaaattgcgc tcacaggttg tcccaatgac
     3061 tgcgccaagg tccgcatgca tgacttcgga attatgggca tgacaatgcc ggtatatgat
     3121 cctagccgct gcgtttcctg cggtgcctgt gaaaaatact gccgcagaaa atcagtccag
     3181 gctatagaaa tggtaaacga aaggccagtc cgtaatgagg aaaaatgtat aggctgcggc
     3241 gagtgcgtcc tcaactgtcc gacccgtgcc tggacccgcg gagaaggtgc agataaacga
     3301 tacaggctga ccttgtttgg ccgtacagga aagcgcaacc cccgcctggg agaggatttt
     3361 atcaaatggg tagatgaaga tagcataata aaaataattc taaacaccta cgactatgtg
     3421 gcaaaataca tagacaaaga cgctcccggc ggaaaggagc atataggcta cattgtcgac
     3481 cgcaccggtt ttgaagagta caaaaaatgg gccctcaaag gagtgaatct cccaccaggt
     3541 gcagttgtgt acaatccgct gtactggaaa ggtataaaat atacagaaat gacactttaa
     3601 cttgagctat tagaagcccg aggaagcaag ggggcggttt cctttgcttc ctacaggctt
     3661 t
//
LOCUS       DAONUO010000207         3653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17665_length_3653_cov_3.317676, whole genome shotgun sequence.
ACCESSION   DAONUO010000207 DAONUO010000000
VERSION     DAONUO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17665_length_3653_cov_3.317676"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1002)
                     /locus_tag="PK768_07690"
     CDS             complement(136..1002)
                     /locus_tag="PK768_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60891.1"
                     /translation="MRIYLTQTSNINSTITSQEVTSAYVTQKLDNGYMVNIGGSEVFA
                     YSSLDFKAGDFLKLKRVESSTSQIIFKVVDHESEAQVLPKALTSFDISHTRETQVAFN
                     LLSELNLPITEERVAFIAELLYHLTNEEKAEPSMPLSKEQPFEALVSTFHKAIADYID
                     NGFILSDNQLLELLENFAAVDKNKVFTFSKALPHKRTKEAVLKEQLALKAINLLHSKD
                     SASNIYFFALPLPIYQNVYLKVSKHSSKDDKETSSLSFIINTKNLGAILVELMYADGK
                     ITHHLPLKIKRQ"
     gene            complement(1102..1902)
                     /locus_tag="PK768_07695"
     CDS             complement(1102..1902)
                     /locus_tag="PK768_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HQA60892.1"
                     /translation="MRKFFTVILAVLLITALVTGCGQKKPQEQAPQEQAGPESEAVSY
                     EDGVYYAEAADFDDHGWKAMVTVIVEGGKISNVFFDEINKEDMLKSFDPEYAANMKDK
                     SGATPLDAYNNLQQSLVSKQNPDNVDAVTGATHSSDTFKDLAKKALEASPVEASGAYK
                     NGLYKAADKDFDDHGWKGMAAVLIRDGKIAIAAYDEINKDGVYKSTDADYANNMKAQS
                     GVTPAEAIDTLTKSVIDKQAADVDSVTGATGTAERFKALMQEALSLAK"
     regulatory      2170..2273
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            2361..>3653
                     /locus_tag="PK768_07700"
     CDS             2361..>3653
                     /locus_tag="PK768_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HQA60893.1"
                     /translation="MKEESTISNAPKSGLEGFFALSENGTNVKTEILAGLTTFVTMAY
                     ILFVNPLILADAGMDRGAVFMATALAAAISTLFMGLYANYPFALAPGMGLNAFFAYVM
                     VGSMGLSWQTALGAVFLSGIIAVIVTLTGLRELLIRAIPISMKHAVGAGIGLFIAFIG
                     LVNSGIVVANEATLVDLGDFTQPGTLLATIGLIIMAVLVAKGIKGGILISIVITSIVG
                     IPMGITQIPSSIISTPPSLAPGLLKLDLAGALKFSMFPVIFSLFFADLFDTIGTFVGV
                     ASRTGMIDEHGNLKRGNKALFADSLGTVVGSLLGTSNTTTYVESAAGVSVGGRTGLTA
                     VVVSLMFVASVILSPIALAVPGEAVAPALIMVGVFMASSLNEIDFSDISEAFPAFITA
                     IAMPMTYSISYGLALGFVAYTAIKFLTGKGKEIHWLMYI"
BASE COUNT          971 a          758 c          776 g         1148 t
ORIGIN      
        1 tccctgtagt tatcggctgc ttttttatat ccccaaagaa gaagtctttt atggaaatcc
       61 tccccacctt cagcttcatg gctttgatga cattcgggat atccctttcc tcatttaaaa
      121 actgcttaac cgcattcatt gcctttttat cttcaaaggt agatgatgcg ttatttttcc
      181 gtctgcgtac atgagttcca caagtattgc tcccagattt tttgtattaa tgataaaact
      241 taaactggaa gtttctttat cgtcttttga ggagtgcttg gataccttta agtaaacgtt
      301 ttggtaaata ggtaagggca atgcaaaaaa gtaaatgttg cttgcagaat ctttgctgtg
      361 caggaggtta atcgctttta gagccagctg ctctttcagg actgcttctt ttgtcctctt
      421 atggggcaga gctttgctga aagtaaatac cttatttttg tcaacagcag caaaattttc
      481 gagaagctca aggagctggt tatccgatag aataaagcca ttatcaatat agtcggcaat
      541 agctttatga aaagtagaaa ctaaggcttc gaagggttgc tcctttgaca aaggcataga
      601 aggctcggct ttttcctcat ttgtcaagtg atataggagc tcggcaataa atgcaacacg
      661 ttcttctgtt atgggtaaat taagctctga caaaagattg aatgcgactt gagtttctcg
      721 ggtatgggat atatcaaagc tcgtcaaggc ttttggtaaa acctgcgctt ctgactcgtg
      781 gtcaaccact ttaaaaataa tttgggatgt tgatgattct actcttttta attttagaaa
      841 gtcgcccgcc ttaaagtcta gcgaagagta ggcaaagact tcagagccgc ctatatttac
      901 catataaccg ttgtccagct tctgtgttac gtaagcgctt gttacttctt gagatgtgat
      961 ggtgctattt atgttggaag tttgggtcaa atatattctc atggtcctac ctcccgaaaa
     1021 aagggcaagc attttcttga ttataacata gaaaaaactt cgggcaaaag cccgaagttt
     1081 caaagttaaa ataagcagta tttatttcgc aagggaaaga gcctcctgca tcagtgcctt
     1141 aaatctttcg gcagttccgg tggctccggt gactgaatca acgtcggcgg cctgtttatc
     1201 aattactgat tttgtcaagg tatcaatggc ttctgcagga gttacgccgg attgtgcttt
     1261 catgttattg gcatagtctg catcggtgct cttgtaaaca ccgtctttat ttatttcgtc
     1321 ataagcggca atggctattt taccatccct aatcaagact gccgccattc ctttccagcc
     1381 gtggtcgtca aaatctttat cggctgcctt gtataagccg tttttataag ctcctgaggc
     1441 ttctaccggc gaagcctcaa gggctttttt agcaaggtct ttgaaggtgt cagagctatg
     1501 ggtggcaccg gttaccgcat ccacgttgtc gggattttgt tttgagacca gcgattgctg
     1561 taaattattg tatgcatcca gcggtgttgc gccggattta tccttcatat tggctgcgta
     1621 ttctggatca aagctcttca gcatgtcttc tttattgatc tcatcaaaaa agacattgct
     1681 aattttgccg ccttccacta ttactgtaac catagccttc catccgtggt cgtcaaagtc
     1741 tgcagcttca gcataataaa caccatcttc gtaagataca gcttcgcttt caggacctgc
     1801 ttgttcttgc ggagcttgtt cctgaggctt tttctgaccg caccctgtaa caagggctgt
     1861 aatcagaagc accgctaata taacagtgaa aaactttctc atttattatc ccctccgtat
     1921 gtgaaagtgt taattatatt atgtgtatat aatttgacaa aaaaacgaaa agtcctgcta
     1981 gaaaataaaa agtgtataaa aatataatcg gagactgatc tacgagtcca aatctgtcta
     2041 aaataatata cataagcagt taaagcattc acgcccctgc aattaacgaa ctttaattat
     2101 aaatttgagc atatagttcg ctttttgtgt tgacaatgcg cgctttctcg gttaaaatat
     2161 tttatagtca agcagatact cttaaaaccc tgtataattt cgggaatatg gctcgaaagt
     2221 ttctaccagg tgtccgtaaa acaccacagg ctacagggga actcatgcat tccccgtttt
     2281 tgcggggatt ttattatttc ttccccttta ggggattaca taatatattt taaggtaaaa
     2341 taaaaagagg aggtttcatc atgaaggagg agagtacaat ttcaaacgca cctaaaagcg
     2401 gactcgaggg tttttttgcc ttatccgaaa atgggacaaa cgttaaaacc gaaatcttag
     2461 caggccttac cacgtttgtg accatggctt acatcctctt tgtaaacccg ctcatacttg
     2521 cagatgcagg aatggatagg ggagcagtct ttatggctac tgctctggca gcggctatat
     2581 caacactttt tatgggcctt tatgctaatt atccctttgc attggcaccc gggatgggac
     2641 ttaatgcctt tttcgcatac gtcatggttg gcagcatggg cctttcctgg cagacagcac
     2701 tgggagcagt atttttatcc ggtatcattg cagtaatagt aaccttgacg ggtcttaggg
     2761 aacttttgat aagagcaatt ccaatttcaa tgaaacatgc tgtaggagca ggtattggat
     2821 tatttatcgc ttttatagga ttggttaatt ccggcatagt ggtagccaat gaagctacct
     2881 tagtagatct tggcgatttt acccagccgg gcaccttact tgcaaccatc ggactcatca
     2941 ttatggcggt tctcgtagcc aaaggaataa aaggcggaat tttaattagt atagttataa
     3001 catcgattgt aggaatccct atggggatta cccaaattcc ctcaagtatc atttcaacgc
     3061 ctcctagcct tgcaccggga ttactgaaac ttgacttggc aggcgccttg aaattctcca
     3121 tgtttccggt catattttca ctcttcttcg cagatctttt tgacactata ggtacattcg
     3181 taggtgttgc cagccgcacc ggaatgattg acgaacacgg aaacttaaag agaggcaaca
     3241 aagcactgtt tgccgattcc cttggaacag ttgtaggttc acttcttgga accagcaaca
     3301 ccacaactta cgttgaaagt gctgcaggtg tcagcgtagg tggcaggact ggtctcacag
     3361 ctgttgttgt atctttaatg ttcgtggcaa gcgtcatttt gtcaccaatt gcactggcgg
     3421 ttcccggtga agctgtggcc ccggcgctaa taatggtagg ggtatttatg gcaagcagct
     3481 taaatgaaat cgattttagt gacatatctg aagcttttcc ggcctttatc acagcaattg
     3541 ccatgccgat gacctacagt atttcctatg gtttagctct gggcttcgtg gcttacacag
     3601 ccattaagtt ccttacagga aaaggcaaag aaattcactg gctcatgtac att
//
LOCUS       DAONUO010000208         3651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17680_length_3651_cov_3.582314, whole genome shotgun sequence.
ACCESSION   DAONUO010000208 DAONUO010000000
VERSION     DAONUO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17680_length_3651_cov_3.582314"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PK768_07705"
     CDS             complement(<1..702)
                     /locus_tag="PK768_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQA60894.1"
                     /translation="MRSSLKTLAFVLLIGVFLLTGVTGCGGSKQAEEIRIGGKNFSEQ
                     FIMAEMLSILIEENTDLKTSVQTNLAANVIFNAIKSNQIDLYLEYTGTGLINLGMEPK
                     TDPDEVYEIVKKEFDEQFNIKWMKPYGFNNTYAMVVTKETAEKYNLKTVSDMAKVADE
                     LVLGCTYVFVERDDGYPGLSKYYGFEFKDVKGMDPALMYQALVEGSVDVISGFATEGR
                     IAAFDLVILEDDKQFF"
     gene            complement(721..1362)
                     /locus_tag="PK768_07710"
     CDS             complement(721..1362)
                     /locus_tag="PK768_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60895.1"
                     /translation="MALFNYFINNPGTVFRLIIEHLQLTGSAILTAILIGVPLGIVLT
                     RNKRLADIMLALTSVFQTIPSIAFFGLLIPFLGIGRRTAILVLFLYALLPIVKNTYTG
                     IKEVSPFMIDAGRGMGMTNMQILRMVELPQSLSVIMAGVRIATVINIGTTTIAAYIGA
                     GGLGELIFTGIQMYRNEMILAGALPAAILAVLADQVLGYVELKLTPRGLRKKK"
     gene            complement(1377..2489)
                     /locus_tag="PK768_07715"
     CDS             complement(1377..2489)
                     /locus_tag="PK768_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60896.1"
                     /translation="MISFKNVTKKYDRFVAVDNLTLDINTGEIVVLIGPSGCGKTTTL
                     RMVNRLIEPTEGTIYIDGTDIGKLDAVELRRNIGYVIQQIALFPHMTIAQNIGLVPYL
                     KNWPEKKRKERVEELLEFVGMPPSQFYNRYPNELSGGQQQRIGVARALAADPDIILMD
                     EPFGAVDPITRTSLQEELLQMHDKLAKTIVFVTHDMDEALKLADKIAIMKDGKVLQYD
                     RPDQLLTNPAHGFVEEFIGKDRLLKQPEFINVKDVMIKNPVTILPERTLTQALEKMRQ
                     EKVDSLMVIDESGSFIGLTTANDVLQNFDRAERVSEILTTNVHHVSENANISQVLSIM
                     AQKQVGYVPVISDDNKLAGLITRSSLVNVLGQRLNS"
     regulatory      complement(2576..2711)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2928..>3651
                     /locus_tag="PK768_07720"
     CDS             2928..>3651
                     /locus_tag="PK768_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQA60897.1"
                     /translation="MKSYKSDMIRNVGLIGHSGSGKTSLAEAMLYNSGAIDRLGKIDD
                     GNTVCDYDPEEIKRKISISTSLAPCEWKNIKINLLDTPGYFDFVGEVKSTLRVVENAV
                     IVACAVSGVEVGTEQVFQYAKEVGIPRMFFINKMDRENANFNKVLEQIREFFGPKAVP
                     FQLPIGSEANFNGVVDIVAQKAYTFDGKNVKETPIPDELKDEMEEYRSMIMEAVAETD
                     DELLMKYLEGEELTQDEIQKGLR"
BASE COUNT          993 a          837 c          785 g         1036 t
ORIGIN      
        1 gaagaactgc ttatcatctt cgagaatcac caaatcaaaa gcggcaatcc tgccttccgt
       61 ggcaaaaccg cttattacat caacacttcc ttcaacgagg gcttgataca tcaaagcagg
      121 atccatgccc ttcacatctt tgaactcaaa gccgtagtac ttgctgagac ccggataacc
      181 gtcatccctt tcaacaaaaa cataggtgca gccaaggacc aattcatcgg ccactttggc
      241 catgtcggat acggttttta aattgtattt ttcagccgtt tccttagtca ccaccatggc
      301 ataggtgttg ttaaaaccgt agggcttcat ccacttaatg ttaaactgtt catcaaattc
      361 ctttttcacg atttcatata cttcatccgg atccgttttt ggctccattc caagattgat
      421 gagccctgtt cccgtatact ccaaatataa atcaatttga ttggatttta ttgcgttaaa
      481 aattacgttg gctgcaagat tggtctgcac acttgtctta aggtccgtgt tttcctctat
      541 gagaatgcta agcatctcgg ccatgatgaa ctgttcggaa aagttcttgc cgccgattcg
      601 tatttcctca gcctgcttcg agccgccaca tccggttact cctgttaata aaaacacacc
      661 aatcaacaac acaaaagcta aggtctttaa gctacttctc attttaaatc ctcctaaaat
      721 ttactttttc ttgcggagcc ctcttggtgt cagcttaagc tctacatagc ccaatacttg
      781 atccgctaaa actgcaagga tagctgccgg taaggctcct gctaatatca tctcatttct
      841 gtacatctgt atacccgtaa agatcaattc accgaggccg cctgcaccga tatatgcggc
      901 gatggtggtg gtgcctatgt ttattaccgt tgcgatcctt actcccgcca ttataactga
      961 cagagactgg ggcaattcta ccatgcgcag tatctgcatg ttggtcatgc ccatgccgcg
     1021 ccctgcatca atcataaagg gggatacttc tttaattccg gtgtaggtgt ttttcactat
     1081 gggcagtagg gcgtagagga agagcaccaa tatggcagtc cgcctgccga tgcccaaaaa
     1141 gggtatgagc aggccgaaga aggctatgct gggaatggtt tggaatacac tggttagggc
     1201 aagcatgata tctgctaaac gtttatttcg agtcaagact atacccaggg ggactccgat
     1261 aagaattgcc gttagtatgg cagacccggt tagctgcaag tgttcaatta tcaggcgaaa
     1321 tacggtgccg ggattattta taaagtagtt aaacagcgcc acctttcaca ctcccttcat
     1381 gaatttaaac gctggcctag gacattgacc aaactggatc tggtaatgag ccccgccaat
     1441 ttgttgtcat cgctgataac cggcacgtag ccaacctgtt tttgtgccat gattgacagc
     1501 acctgactta tatttgcatt ttcggataca tggtgaacat tcgtcgtcag tatttcggat
     1561 accctttctg ccctatcgaa gttttggagc acatcattgg ccgtagtcag gccgatgaaa
     1621 ctgcctgatt catctatgac catgaggctg tcaacctttt cctggcgcat cttttctaaa
     1681 gcctgggtca aagtcctctc gggaagtata gtgacgggat tttttatcat aacatctttt
     1741 acattgataa actccggctg cttgagcagc ctgtccttgc cgataaattc ttccacaaaa
     1801 ccgtgggcgg gatttgtcaa aagctgatcg ggcctgtcat attgaaggac cttaccgtct
     1861 ttcatgatgg cgattttatc tgcaagtttc aaggcttcgt ccatgtcgtg ggttacgaag
     1921 acgatggtct ttgccagctt atcatgcatc tgcagcagct cttcctgtaa gctagtccgg
     1981 gtaatggggt caacggcccc aaaaggctca tccattaata tgatatcagg atctgccgcc
     2041 aaagccctgg ccacaccgat tcgctgctgc tggccgccac ttagctcgtt gggatagcga
     2101 ttgtaaaatt gagaaggtgg catgcctacg aactccagca gttcctcaac ccgctccttt
     2161 cgcttctttt ccggccagtt ttttagataa ggcacgaggc cgatattttg agcaatggtc
     2221 atgtgcggga aaagggcaat ctgctgtatg acatagccta tgtttcgcct gagctctacc
     2281 gcatccagct tgcctatgtc agttccgtca atatatatgg ttccttcggt tggttcaatt
     2341 aggcggttga ccatgcgcaa ggtagttgtt ttgccgcatc cgctgggtcc aatcaggacg
     2401 acaatttctc cagtattaat atccaatgtt aaattgtcaa cagccacaaa cctgtcgtac
     2461 tttttcgtta catttttaaa acttatcaag aaaaaacctc ccttctctcc tttaatacgc
     2521 agaaaaggcc atggagatcc atagcctttg gaaataaaaa atatatttat actatcaatc
     2581 tcctcttccg acgcttacga ggttagctga cgggttcagg ccgaagagta gccctacttc
     2641 ttgcgaagat gcaccccaaa aagtggttcc cccgctccgg tcacgttgac cgaattcagc
     2701 gaatagctaa acgatttttt tttacgtttt ttattataac aaatataaaa aagctgtcaa
     2761 ccttagttgc ccaaagatgc cgtttgtgat atcatttcta taataaagat tttccataat
     2821 tccttagcag gaaaaccctt tgcataagta gaataaatat atttaattaa agcatatatt
     2881 ttacatatat tttatcggta agtcaactat taaggaggag atcaggtttg aaaagctata
     2941 aaagtgacat gataaggaat gtaggtctaa ttggtcatag cggttccgga aaaacatccc
     3001 tagcagaagc tatgctgtac aattctggtg ccatcgatcg cctgggcaaa attgacgatg
     3061 gcaatacagt ttgcgactac gatccggaag aaataaagag gaaaatttcc ataagcactt
     3121 ccctcgcacc ctgtgagtgg aaaaatataa agataaattt acttgatact ccgggatatt
     3181 tcgattttgt tggtgaagta aagagcactt tgagggttgt ggaaaatgct gttatagtag
     3241 cctgtgccgt atccggcgtt gaagtaggta cagaacaagt atttcagtat gccaaggaag
     3301 tcggcatccc ccgcatgttt ttcataaaca agatggatcg ggaaaatgcc aatttcaata
     3361 aggtgctaga gcaaattcgc gaatttttcg gtcccaaggc cgttccattt caacttccca
     3421 taggttcgga agcaaacttc aatggcgtag ttgacattgt ggcacaaaaa gcctatacct
     3481 ttgatgggaa gaatgtaaag gaaaccccca tccccgatga gctaaaggac gagatggaag
     3541 agtaccgctc gatgataatg gaagccgtag cggagaccga tgacgagctg ttgatgaaat
     3601 acctggaagg tgaagagctg acgcaagatg aaattcaaaa gggcctgcgc a
//
LOCUS       DAONUO010000209         3640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17741_length_3640_cov_4.220642, whole genome shotgun sequence.
ACCESSION   DAONUO010000209 DAONUO010000000
VERSION     DAONUO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17741_length_3640_cov_4.220642"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..888)
                     /gene="istB"
                     /locus_tag="PK768_07725"
     CDS             complement(118..888)
                     /gene="istB"
                     /locus_tag="PK768_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HQA60898.1"
                     /translation="MIEFEKARGHLEELGLSNAVVYLDALLEKAQRENRTYIDFLNDL
                     LEAEVAERQKRNMEVRTKLARLPYRKTLAEFDFSFQPSIDEKLVKELATMAFIYRTEN
                     VILLGPPGVGKTHLAIGLAIEALSQGISVYFSSLTRLIDDLRKAYDENRLEKRMRIYI
                     RPKLLIIDEVGYLPLDNKGANLFFQLISARYERGSIILTSNKGFGEWGELMGDPVLAS
                     AVLDRLLHHAHTINIRGNSYRLKDRLRTGFYASPHSNV"
     gene            complement(885..2114)
                     /gene="istA"
                     /locus_tag="PK768_07730"
     CDS             complement(885..2114)
                     /gene="istA"
                     /locus_tag="PK768_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HQA60899.1"
                     /translation="MLGSGSIIMLHELEAKGKSIRAISRETGLSRNTVRKYLRAAGIP
                     ERKPHPQRGSKLDPYKDIIQEMINSGIFNCEVIYERIRDEGYTGGRTILRDYVRPFRP
                     RKQVLAVPRYETGPGRQAQVDWGEYTYIDEVTGEAHKLYVFVMILSYSRAIYVEFTKR
                     CNVRTFIRCLIHGFEYFGGVTDIVLTDRMKTVILGTDENRQPIWNAVLKDFAVTLGFI
                     PRVCRAHRPQTKGKVESGVGFVKNNFLPGRRFADYGDLNRQAVEWCDKKNRRIHGTTG
                     VRPVERLKEENLKPLPTPDIYQKFIDEERQVYKDGLLSFDGVMYGIPWKYSGRKVIVR
                     EKDGKIGIFCDGKVIAVHEKRYRSKDTVFLKDQYKGLKDAEGVIYPRPAAIRVFPLEV
                     EKRSLGVYQSLLEADQS"
     gene            2285..3019
                     /locus_tag="PK768_07735"
     CDS             2285..3019
                     /locus_tag="PK768_07735"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HQA60900.1"
                     /translation="MQININKAKYPFEYSRDRRLNFTIAHEIGHIVLEHLLIPRSLKT
                     GIELFIEEHEANEFAGRLLMPEKMLCSCNYYSIDSVAQYFIVSRSALWKRLNNMKRLD
                     LLNSRKIQACSRCGNTRFSVFAGFCGICGQPIGNKLSGIRRVYYPEVYPMDQCKRVTS
                     CPRCNNDLRRNVYDKCSICGTHIFNFCSSFFDGNVDECSYANPGNCRFCEMCGRPTYF
                     FEKGLLKPWQEADDFCYVAESRGEYI"
     gene            complement(3226..>3640)
                     /locus_tag="PK768_07740"
     CDS             complement(3226..>3640)
                     /locus_tag="PK768_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60901.1"
                     /translation="CLIDYLVYRVIGKEFCKESLSIFECETKQHTFKWHSARNRTCQV
                     CYKKGINNKAHVIKKVLPCTDSEGYLVIQNSAFVSANNALLLNIKECPFVSVCESKTN
                     NFRKLNPPKSISILGQTGWETAKTRSIEGGGGLMS"
BASE COUNT          957 a          869 c          700 g         1114 t
ORIGIN      
        1 ccatgcagaa gaaatatatt cgtacaattc gcccgtacat atccttaagt ttgtcaataa
       61 cggactaaat ctgacccgcc aacaacggat aaaatttgac ccaccccggt aaaaaaatca
      121 aacgttgctg tgagggctgg catagaaccc agttctcagc ctatccttta agcggtaact
      181 attgccacgt atgttgattg tatgagcatg gtgcaatagc ctgtccaata ctgccgaagc
      241 aagtacagga tcgcccataa gttctcccca ttctccaaag cccttattgc ttgtcaggat
      301 tatgctccct ctctcgtaac gggcacttat caactggaaa aagaggtttg ctcccttgtt
      361 gtccagaggc aaatatccca cctcgtcgat tatgaggagt tttggcctga tatatatcct
      421 catgcgtttc tccagccgat tttcatcata tgccttcctg aggtcatcaa taagccttgt
      481 aagactgctg aaatacactg atatcccctg cgacaatgct tctatggcca gtccaatggc
      541 caaatgtgtt ttacctaccc caggaggccc cagaagtatc acgttctcag ttcggtatat
      601 aaaagccatg gtcgcaagtt cttttaccag cttttcgtca atactgggct gaaaactgaa
      661 atcgaactcc gctaatgttt tcctgtatgg cagccgtgcc agctttgtcc ttacctccat
      721 gttgcgtttc tgtctttcag caacttcggc ctcaagaaga tcatttaaaa aatcaatata
      781 tgtcctgttt tcccgttggg ccttctcaag gagggcgtca agataaacca cagcgtttga
      841 aagccctaat tcttcaagat gtcctcgtgc tttctcaaac tcgatcatga ctgatcagcc
      901 tccagcagac tctgataaac acccagcgaa cgtttctcga cttccagggg aaataccctg
      961 attgctgccg gccttgggta tatcacgccc tcggcatctt taagcccttt gtattggtcc
     1021 ttgaggaaaa ccgtgtcctt tgaacggtat cgcttctcgt gaacagctat gaccttccca
     1081 tcgcaaaaga tccctatctt gccatctttc tccctcacga tcaccttcct cccgctgtat
     1141 ttccacggta tgccatacat cacaccgtca aagcttaaaa ggccgtcctt gtacacttgc
     1201 ctctcttcat caatgaattt ttggtatatg tcaggggtag gcaggggttt gaggttctct
     1261 tctttcagac gctcaaccgg cctcactcct gtcgtaccgt gtatcctcct gttcttctta
     1321 tcgcaccact ctaccgcctg gcggttcagg tccccatagt ctgcaaacct ccttccaggc
     1381 aaaaagttgt ttttaacaaa tcctacgcca ctttccactt tgcccttggt ctgtggacga
     1441 tgtgctctgc ataccctggg aataaaccct aaagtcaccg caaaatcctt caaaaccgcg
     1501 ttccatatcg gttgcctgtt ttcatcggta ccaagtatta cggtcttcat tctgtctgtg
     1561 agtactatat ccgttacccc gccaaagtac tcaaatccgt gaatgaggca tcgaatgaaa
     1621 gtacgcacat tgcagcgttt cgtaaattct acatatatgg ccctggaata actcaatatc
     1681 atgacaaaaa cataaagctt atgcgcttca ccggtaactt cgtcaatgta tgtatattca
     1741 ccccagtcca cctgggcttg ccggccggga cccgtttcat agcgcggtac agccaaaact
     1801 tgtttccgag gtctgaacgg ccttacataa tccctcagga tagtacgtcc gcctgtatag
     1861 ccttcatccc ttatcctttc gtaaatgact tcacaattaa atatgcctga gtttatcatc
     1921 tcctgaataa tatctttgta cggatcaagc tttgaacctc tttgcggatg aggttttctt
     1981 tctggaatac cagctgctct taaatatttc cttacagtat ttcttgaaag tccggtttcc
     2041 cttgatattg cccgaatgct cttgcccttt gcttctaatt catgtaacat gataattgat
     2101 ccactcccta gcatttattt ccctccgatt cttttggttt atcagaggga taattcgcca
     2161 ggggtgggtc attttctttc cgttacttcg gggtcaatta tataccgtcg ttgacagcta
     2221 cgtcccactg ccaaataaat atgacgcttt aaccgggtat gtagattctg aaggtgtgta
     2281 tataatgcaa atcaatatta ataaagccaa atatcccttt gagtattcaa gggaccgcag
     2341 gcttaatttt acgatagctc atgaaatcgg gcatatcgtg ttggaacacc tgcttatacc
     2401 aaggagcctg aagaccggta ttgagctttt catcgaggag catgaagcaa acgagtttgc
     2461 tggaagattg cttatgccag aaaaaatgtt gtgcagctgc aattactatt ccatagattc
     2521 ggtggcacag tattttattg tttccaggtc tgccctttgg aagcggctaa ataacatgaa
     2581 acgtctagat ctactgaatt cacggaagat acaagcttgc agccggtgtg gaaatacacg
     2641 tttttctgta tttgccggat tctgcggtat ttgtggacaa cccattggca ataaactaag
     2701 cggcatccgc agggtatatt atccggaagt atatccaatg gatcagtgca aacgggtaac
     2761 atcctgcccc agatgcaata atgatttgcg gagaaatgtc tatgataaat gcagcatatg
     2821 cggcactcat attttcaatt tctgcagcag cttctttgat ggaaacgttg atgaatgctc
     2881 atatgccaac cccggcaact gccgtttctg cgaaatgtgc ggccggccga cttatttctt
     2941 cgaaaaaggg ctgctgaagc cttggcagga ggcagatgat ttttgttatg tagctgaaag
     3001 taggggtgag tatatttgat cacactacaa gagccaaata tactacatat tgcaaaatta
     3061 gcggcagtta aacctatttt tgccatgggc ttttataaac cttgagagga aaaccagtca
     3121 tttatgctgg caggtactgt tgctgaggac actttggagt tgtaacatca acagcttctt
     3181 tcatagtata tttaaaagtc attttcacca ttccttaatg gttatttaag acatcaaacc
     3241 tccaccacct tcaattgacc ttgtttttgc agtttcccaa ccggtttgtc ctaaaatact
     3301 gatagatttt ggcggattta attttctgaa attattagtt tttgattcac aaactgacac
     3361 aaaagggcat tccttaatat taagtaaaag agcattattt gcagaaacaa acgcgctatt
     3421 ctgaattact aaataccctt cggaatctgt acagggtaaa acttttttaa taacgtgagc
     3481 tttattatta atcccttttt tataacaaac ttgacatgtt ctgtttcttg cagagtgcca
     3541 tttgaaggta tgctgtttag tttcacattc aaatattgat aagctttctt tgcaaaattc
     3601 ttttccaata actctgtaca caaggtaatc tattaaacac
//
LOCUS       DAONUO010000210         3627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17811_length_3627_cov_4.319149, whole genome shotgun sequence.
ACCESSION   DAONUO010000210 DAONUO010000000
VERSION     DAONUO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17811_length_3627_cov_4.319149"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK768_07745"
     CDS             <1..209
                     /locus_tag="PK768_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HQA60902.1"
                     /translation="ANEVITLIKDTALVYVVGLGELLRAGKIASNRDASLVPLLLVGI
                     IYLALTAILTTAFQKLEDRYSYYQ"
     gene            222..968
                     /locus_tag="PK768_07750"
     CDS             222..968
                     /locus_tag="PK768_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HQA60903.1"
                     /translation="MVLEVQGLYKSFSGEPVLQDVSFNLNQGEILAILGRSGAGKTTI
                     LRCINNLTKCDRGTIKVDDMYLCREENGATVYANPSTMLKIRKNLGMVFQNFNLFPHM
                     SVVQNIVEAPINVFGMPKQKAHEKAAELLEMLGLSDKADSYPFELSGGQKQRVAIARA
                     CALNPKIMCMDEPTSALDPELREGIASIIENLAKDGMGILIITHDMNFAKRVATRIIF
                     LDDGKVIQESSKEEFFESMDNERIRRFVAM"
     gene            complement(1012..1743)
                     /locus_tag="PK768_07755"
     CDS             complement(1012..1743)
                     /locus_tag="PK768_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HQA60904.1"
                     /translation="MYQKRFYRDYMISQGLVRFNVTVFESDLEIFAKCNLENEAKKLL
                     KQYRAEIQEYIDRHPEFLTSLLPIKARDNAPGIVKHMCLAAEAAGVGPMAAVAGAISQ
                     YVGMALGEYSDEIIIENGGDIFMKNKTDKHILIYAGRSPLSNKMALQIRGENKTVGIC
                     TSSGTVGHSLSFGKADAAVVISRDTLLADAVATAVCNMVKDGNDIEESIDFAKSIHGI
                     DGVLVIIGDSLGAWGKIHLVDPGDL"
     gene            complement(1787..1993)
                     /locus_tag="PK768_07760"
     CDS             complement(1787..1993)
                     /locus_tag="PK768_07760"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA60905.1"
                     /translation="MSKIIVEYAKCVECGACTAVCNTNALQLDASSWSLVYDKRKCTG
                     CKLCIKACPLRAIRASAKLSSAAT"
     gene            complement(1997..3166)
                     /locus_tag="PK768_07765"
     CDS             complement(1997..3166)
                     /locus_tag="PK768_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HQA60906.1"
                     /translation="MSVKKSFEEINEKIKKGTVVVVTAEEVIDIAKEKGIKDATKYVD
                     VVTTATFGPMCSSGAFLNFGHADPPIKMAEVQLNNVTAYAGLAAVDAYIGATEPSKDR
                     GIEYGGAHVICDLIEGKKVHLKAKSPGTDCYPRKEIDTYVTKDSINEAYLFNPRNCYQ
                     NYNAAINTSDRTLFTYMGVLQPNKGNINYSTSGELSPLLNDPYLRTIGIGSKIFLAGT
                     TGYVSWQGTQFVNAVPRSELGIPFSPAATLAVIGDLKQMNGEYIKPAVFEKYGTSLYV
                     GMGIPIPVLDEDLMINLAVENKDIFTNIIDYSVPRRSRPSLGRVSYEELRSGTVTLEG
                     KKIPTAPLSSLSKARQIASELKSWIQNNKFTLQEPIKPFDEVASLNTLEEIDERS"
     regulatory      complement(3277..3387)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          962 a          794 c          796 g         1075 t
ORIGIN      
        1 tggccaatga ggtgattacc cttatcaagg acacggcctt agtatatgta gtgggcctgg
       61 gtgagctgct gcgtgccggt aaaatcgcat ccaatagaga tgcttcttta gtgcccttgc
      121 tgttggttgg aattatttac ttagcactga cagcaatttt gacaacggcc tttcaaaaac
      181 tggaagatag gtattcatat tatcaataag gagaaagaca aatggtgctt gaagttcagg
      241 gcttgtacaa gagcttttcc ggggaaccgg ttttacaaga tgtcagtttt aacctaaacc
      301 agggagagat attggcgata ctggggcgct ccggtgccgg taaaaccact attttgagat
      361 gcataaacaa tttaacgaaa tgcgaccggg ggacaatcaa agttgacgac atgtacctgt
      421 gcagggaaga aaacggcgcc accgtttatg ccaatccgtc aaccatgctt aaaataagga
      481 agaatctggg catggtcttc caaaacttta acctgtttcc acacatgtcg gttgtacaaa
      541 atatcgtgga agcgcccatt aatgtcttcg gtatgcccaa gcaaaaggcc cacgaaaagg
      601 ctgccgagct tttagaaatg ctgggtctca gcgataaagc cgactcttat ccctttgaac
      661 tttccggcgg tcaaaagcag cgggtggcca ttgccagggc ttgtgcctta aatcccaaga
      721 tcatgtgtat ggatgagccc acttctgcac tggacccaga attaagagaa ggcatcgctt
      781 caattatcga aaatctagct aaagacggga tgggaatatt aataataacc catgacatga
      841 attttgcaaa gagagtagca actagaatca tatttttaga tgacgggaag gttatccagg
      901 aaagcagcaa agaagagttc tttgaaagca tggataatga gcggataaga cgatttgtgg
      961 ctatgtaaca tggcatgtaa ggggtttgct tcggcagacc cctttttacg cttataagtc
     1021 tccgggatcc accaggtgta tttttcccca ggcgcctaag ctatccccaa taatcacaag
     1081 gacgccgtca atgccatgaa tggattttgc gaaatctatg ctttcttcaa tatcattccc
     1141 atcctttacc atgttgcaaa ctgccgtggc tacagcatct gctaaaaggg tgtcccttga
     1201 tataactact gctgcatctg cctttccgaa gcttaaggaa tggcccacgg ttccgcttga
     1261 tgtgcatatc cccacggttt tattttcccc ccgaatctgt aaggccattt tgttggaaag
     1321 gggcgacctg ccggcatata taagtatatg cttgtccgtc ttatttttca taaagatgtc
     1381 gccgccgttt tcgatgatga tttcatcgct atactcgcct aaggccatgc cgacatattg
     1441 ggaaattgca cctgccactg ctgccatagg gccaacgcct gcggcctcag cggcaaggca
     1501 catgtgcttt acaattccag gtgcgttatc ccgagcttta attggaagca gacttgtcaa
     1561 gaactcgggg tgacgatcta tatactcttg tatctcagcc ctgtattgct ttagcagttt
     1621 ctttgcttca ttttcaagat tgcattttgc gaagatttcc aagtccgatt caaataccgt
     1681 tacgttaaac cggactagac cttgtgatat catgtaatcc ctgtaaaagc gtttttgata
     1741 catcttatca cctaaatagc gatagtaaca tcagtttttt ccgtgattac gttgcggcag
     1801 aagagagttt agccgaggcc cttattgccc gtagagggca ggctttgata cacagcttgc
     1861 agccggtgca tttacgctta tcataaacta gcgaccaaga actggcgtct agctgtaagg
     1921 cattggtgtt gcacactgcc gtgcaggctc cgcattccac acattttgca tattctacga
     1981 ttattttgct catgatttag ctcctttcat cgatttcttc cagggtattt aaacttgcca
     2041 cttcatcgaa gggttttatc ggctcttgca gagtaaactt gttattttgt atccagcttt
     2101 ttagctctga agcaatttgt ctcgccttgc tcagacttga gaggggtgct gtaggaatct
     2161 tttttccttc taaggttacg gtgccgcttc tcagctcttc gtagctaact ctacccaagc
     2221 tcggccttga acggcgggga acgctgtagt caataatatt cgtgaatata tctttatttt
     2281 caacagccaa atttatcatc aggtcttcgt cgagcaccgg tatcgggatg cccatgccta
     2341 catacaggga agtcccgtat ttttcaaata ccgccggttt aatatattca ccgttcattt
     2401 gctttaaatc tccaatcacc gccaaagtgg ctgccggaga aaagggaatc cccagttcgg
     2461 accttggcac cgcatttaca aattgggttc cctgccatga tacatagccc gtcgtgcctg
     2521 ccaaaaagat tttgctgcct atgcctatgg tcctgagata agggtcattg agcaatgggc
     2581 tcagttcacc ggaagttgag taatttatat ttcccttatt cggctgcagg actcccatgt
     2641 aagtgaaaag ggtcctgtct gaagtattga tggccgcatt gtagttttgg tagcaatttc
     2701 taggattgaa taaataggcc tcatttatgg aatcctttgt tacgtaggtg tctatttcct
     2761 tcctgggata gcagtcagtg ccgggagatt tggctttaag gtgaaccttt ttgccttcta
     2821 ttagatcaca tattacatgg gctccaccgt actcgatacc tctatctttt gatggctctg
     2881 tcgcccctat atatgcatca acggctgcaa ggccggcata tgccgtgaca ttgtttaact
     2941 gaacttcagc catttttatg ggagggtctg cgtggccgaa gtttaaaaat gctcccgatg
     3001 agcacatggg accaaaagtg gcagtggtaa ccacatcaac gtatttggtc gcatctttaa
     3061 ttcccttttc cttggcaata tcgattactt cctctgccgt gacgacaact accgtccctt
     3121 tcttaatttt ttcattgatt tcctcaaagg atttttttac tgacatttga gcaacctccc
     3181 ttgctggttt tattaaaaaa ttaagctgct tttcgataag aaaagcagct tgtaggcccg
     3241 ataaacatgg caataaaata ggctaacagg ctgttaatct tatctctcag gataataatc
     3301 ctgcaggaat tgacaccgct gcattcaatt aggaatgccg gttgtcgagc ttcattgggc
     3361 cagtccctcc gctactcttg ataagattat tgcgtttatt taattattgc tatatatgat
     3421 atttgattta aaacagtttg tcaagtgatg gataaaaaaa ttttaaagca gcctcttaaa
     3481 atttgctttc agacagcttg tggtataatc aaataaggca gtaatactcc aagaataaga
     3541 ggtaaaacag ctatgaatat tgatatcatt gcggtaggca agataaagga aaactttttg
     3601 acaaagggca taaaggaata tcaaaaa
//
LOCUS       DAONUO010000211         3611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17893_length_3611_cov_4.591957, whole genome shotgun sequence.
ACCESSION   DAONUO010000211 DAONUO010000000
VERSION     DAONUO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17893_length_3611_cov_4.591957"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            522..719
                     /locus_tag="PK768_07770"
     CDS             522..719
                     /locus_tag="PK768_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60907.1"
                     /translation="MRKIIVNSTPIIAFNKIGKLDLLKKVYGKLLIPYAVYEEVISGL
                     KDSKDNDFKRKWLHRNNSNQE"
     gene            778..996
                     /locus_tag="PK768_07775"
     CDS             778..996
                     /locus_tag="PK768_07775"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HQA60908.1"
                     /translation="MAKEIGADLCIIDDLLARKYAKFYGLKITGTMGVLLKAKQLKLI
                     TEVKPIMDELIASGIYIDNNFLELANED"
     gene            complement(1056..1280)
                     /locus_tag="PK768_07780"
     CDS             complement(1056..1280)
                     /locus_tag="PK768_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HQA60909.1"
                     /translation="MNQTQNIAGQNADKDILTDMLMTEKYVSGTYETAIQESANEAVR
                     NALRQIQDEEQQHAKMIFDAMNQRGWYKPQ"
     gene            complement(1305..1502)
                     /locus_tag="PK768_07785"
     CDS             complement(1305..1502)
                     /locus_tag="PK768_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQA60910.1"
                     /translation="MQLTQKEILYLKDALSHEHICVTKYNNYANELQDAELSAMFRNL
                     ANREQQHIDTINKLLQQGGAQ"
     gene            1675..1941
                     /locus_tag="PK768_07790"
     CDS             1675..1941
                     /locus_tag="PK768_07790"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQA60911.1"
                     /translation="MKLRKNIGELDSLVRLGLGFSMLGYGITKKSNFAILMGSWKIAE
                     GITRVSIMYHLLGIDTLDDELNLSGTKELMLNPLENIAKVDDTE"
     gene            2006..3157
                     /locus_tag="PK768_07795"
     CDS             2006..3157
                     /locus_tag="PK768_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQA60912.1"
                     /translation="MKEIYLDNSSTTKVLEEAAQAVFDAMTINFGNPSSLHRKGIEAE
                     KIMKQARENIARVLGVSPGEIYFTSGGTESNNWAIKGAASALRRRGNHLVTTTIEHPS
                     VLEAFAALEEEGFSVTYLKVDSEAVIDLKELEAAIRPDTILVSIMYVNNEVGSIQPIG
                     EAAQIIKSRSNALFHVDGVQAFGKIPLISNLEGIDLLSISGHKIYGPKGIGALFIRDK
                     VTINPLLHGGGQEAGQRSGTENLPGIAGFGVAANICLEKMSEWQGLMTQLKQRLMRGI
                     LDNIPDTVLNGPASGAPHILNISFLGTRGEILLHALEAKNIYVSTGSACSSRKSAKSH
                     VLKAMAKSQEEQDGAIRFSLSPFISEEDIDYTIEILAKEVREIRKFVRR"
     gene            3160..>3611
                     /locus_tag="PK768_07800"
     CDS             3160..>3611
                     /locus_tag="PK768_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HQA60913.1"
                     /translation="MENLYLISFGEIALKGENRPYFERFLMQRIKEALIPYDKNVQLK
                     KTHGRMYCYTDAPQENVLNALKKVFGIVYISPAKSCENDLEQIKKTALELMKSQDYSG
                     KTFKVETRRPNKSFPLKSPEVSREVGAHILRNLQGLKVDVHEPDILLEV"
BASE COUNT         1188 a          659 c          764 g         1000 t
ORIGIN      
        1 aatgacaaac gagaaatctt tacaaaattt ggcgtgctca cataccacag gaccttttaa
       61 catgggtaaa aaaactccaa tatcaacaat tttaaagggt atacaacgca gcgggtatgt
      121 gatatagctc gttgtatcaa aaagatgggg atacctttaa gtttaattat cctacaacaa
      181 attgacatta tcaataattc caaaggttct ttttaagata tttaataatt gatataataa
      241 tgtgtagaag aagggattga aaggatgtga ttttgatgca atgttatact ataactcttc
      301 ctcaagagat attgctatcg ttaagagaga gcggagagaa tatcgttaaa gacatgaaaa
      361 gaactgttgc tataaagtac tataaagaga aaaaattatc tttaggtcaa tgtgcacagc
      421 tagccgaaat gacaaaggaa gattttatca agttgctatc aataataaaa tatcaatctt
      481 taattttgaa aacgaaaaag aattgctaga ggatatccaa aatgcgtaag ataattgtga
      541 actctacacc tataatagca tttaacaaaa taggcaaatt ggatttattg aaaaaggttt
      601 acgggaaact gcttataccc tatgctgttt acgaagaagt aatttcaggg ctaaaggata
      661 gcaaggataa tgattttaaa agaaagtggc tccatagaaa taattccaat caagaatgag
      721 gaagccagaa gattttttgc aacgagctta cataaaggtg aagtggaggt aatgatcttg
      781 gcaaaagaaa tcggagcaga cttatgtata atagacgatt tattagcaag gaaatatgca
      841 aaattctatg gacttaaaat aactgggacg atgggtgtac tacttaaagc caagcaatta
      901 aaactcataa cagaggtaaa accaatcatg gatgaattga ttgcttcagg catatatatt
      961 gataataatt ttttagagct tgctaacgaa gattaaggat tagcaagctc tttgttgtgc
     1021 gcccggcacc taacttatta acttgttgag atttgtcatt gtggcttgta ccagccacgc
     1081 tgattcatgg catcgaaaat catcttggca tgctgctgct cttcatcttg aatctgcctc
     1141 agcgcatttc taactgcctc atttgccgac tcctgaattg ccgtctcgta tgttcccgaa
     1201 acgtactttt cagtcataag catatcggtc aagatatctt tgtcagcgtt ttgcccggca
     1261 atgttttggg tttgattcac taattatccc tcctaaacct tgttttattg agctccacct
     1321 tgctgcagaa gcttgttgat tgtgtcgatg tgctgctgtt cgcgattggc tagatttcta
     1381 aacattgcag aaagctccgc atcctgcaat tcgttggcat aattattgta cttcgtcaca
     1441 caaatatgtt catggctcag ggcatctttc aaatacaaga tttccttttg tgtaagctgc
     1501 aataatattc acctccaatt tttgttctga cagtcttatt ttatccccta taaaaattac
     1561 tatacttgtt atttattgtg agattaaata caaattaaaa aatctatcca caaatactta
     1621 aaatgggctc aatgattaat actaatagta atacaaacca tggaggagat ttatatgaag
     1681 cttaggaaaa acattggtga attagacagc ttagttcgtt taggcttggg tttttcaatg
     1741 ctgggctacg gcataactaa aaagtcaaac tttgcaatac tgatgggctc ctggaaaata
     1801 gccgaaggaa ttactagagt cagcattatg taccatttgc taggtatcga cacattagac
     1861 gatgaattaa acctttcggg gactaaggag ttgatgttaa accctcttga gaatatagca
     1921 aaagtagatg acaccgaata aacttgtgat ataattataa gggcggatta taccgccctc
     1981 atacatatgc tttgaaggtg atattttgaa agagatttat cttgacaaca gctcgacaac
     2041 taaagtttta gaagaagccg ctcaagctgt ttttgatgcg atgacgataa atttcggcaa
     2101 cccttcctcg cttcacagga aaggcattga ggcagaaaaa ataatgaagc aggccaggga
     2161 aaatattgcc cgggttttag gagtcagtcc cggagaaata tattttacct caggagggac
     2221 ggagtcgaat aattgggcaa ttaagggtgc tgccagcgcc ctccgtagaa ggggcaatca
     2281 tctagttaca accaccatcg aacatccttc ggtgctggaa gcctttgccg ccctggaaga
     2341 agagggtttt tccgttacat atctaaaagt ggacagtgaa gctgtaatag atctaaaaga
     2401 actagaagca gcaataaggc ctgacacgat tttagtcagc ataatgtatg ttaataatga
     2461 agttggctcc attcagccaa taggggaagc agcccaaatc ataaagtcaa ggagcaatgc
     2521 cctctttcat gtggatgggg ttcaggcatt tggcaagata cccttaatct caaatcttga
     2581 aggcatcgat ttgttatcaa tcagcggcca taaaatctac ggtccgaaag gtataggtgc
     2641 tctgtttata agagacaagg tgaccataaa tcccctccta cacggtggtg gccaggaggc
     2701 ggggcagcgc tccggcactg aaaacctgcc gggaatcgcc ggatttggcg tggctgcaaa
     2761 tatttgttta gaaaaaatgt cagaatggca agggctaatg acacaattaa aacagcgttt
     2821 gatgaggggt atattggaca atatcccgga tacggtgcta aatggtcccg cctcgggtgc
     2881 accccacatt ctcaacatct cttttctcgg tacccgaggg gaaattttgc tgcatgccct
     2941 tgaggcaaaa aacatatacg tgtcaacagg ttccgcctgt tcatcgcgca aaagtgcaaa
     3001 aagtcacgtc ctgaaagcca tggccaaaag ccaggaagaa caggacggtg caattaggtt
     3061 tagtttatca cccttcattt cagaggaaga tattgattac actattgaaa tccttgccaa
     3121 agaagtaagg gagataagaa aatttgtgcg gaggtaaaaa tggaaaatct ttatttaata
     3181 agctttggtg agatcgcctt aaagggcgaa aacagaccat attttgaaag atttctcatg
     3241 cagagaatca aagaggcttt aataccttat gataaaaatg tacaacttaa aaagactcac
     3301 ggtcgaatgt actgctatac ggacgccccg caagaaaatg tgttaaatgc tttaaaaaag
     3361 gttttcggca ttgtctacat cagtcctgca aaaagctgtg aaaacgactt ggagcagata
     3421 aagaaaacgg cccttgaact gatgaaaagc caagactata gcggcaagac ctttaaagtg
     3481 gagacgaggc gtccaaacaa atcctttccc ctcaaatccc cggaagtcag ccgtgaagtg
     3541 ggagcccaca tattgagaaa tcttcagggc ctaaaggttg atgtccatga accggacatt
     3601 ctgcttgaag t
//
LOCUS       DAONUO010000212         3596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_17989_length_3596_cov_4.035301, whole genome shotgun sequence.
ACCESSION   DAONUO010000212 DAONUO010000000
VERSION     DAONUO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17989_length_3596_cov_4.035301"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PK768_07805"
     CDS             <1..1030
                     /locus_tag="PK768_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQA60914.1"
                     /translation="WGCLKLRCPQKSERSYYLQDELGSPRRLLGETGSTKALYSYDAF
                     GKPIEPINPFSHIATIDNIYSYTGYQYDISTGLMYAQARYYMPETGRFISRDPYKGNV
                     VDPQSLNLYLYCKNNPILYFDPSGYDAVLDQYISDNFDGKITVTFIVAQPMPGSRLAI
                     DADFSGDVGHTFIRLDFGDGRVIYKGFYPKNALTPEQIINKENVPGIIRDDSEHGWDV
                     AQVFVIDQKQAEKIEKFINNYEKEYNMVRNNCTTFAVDTLKSAGIKSPTSARIWTLPD
                     GAQKLLVDNLPEEMWFKNIVAWWLLHGLKGYSPADAGEDIRENECAIINTNGTVSPVS
                     TNSGGKKR"
     gene            1027..1380
                     /locus_tag="PK768_07810"
     CDS             1027..1380
                     /locus_tag="PK768_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60915.1"
                     /translation="MSKKVILIVIIAIGIVLAFAVVRNGVLLKQSGLNTKLKIGKELY
                     INVADNEIIDADYLSKVANGTYDSAFALMDSKEMHKLIQYLKENNLLIEPGKYVINQA
                     WSFEKVKDILRFKKR"
     gene            complement(1763..1942)
                     /locus_tag="PK768_07815"
     CDS             complement(1763..1942)
                     /locus_tag="PK768_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60916.1"
                     /translation="MAKSNKRRAIFPSDDALMKMLYLVTQDVMKKWTGRVHNWGQILL
                     QLSIYFPDQVTNYLP"
     gene            2498..>3596
                     /locus_tag="PK768_07820"
     CDS             2498..>3596
                     /locus_tag="PK768_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HQA60917.1"
                     /translation="MKIIMKRSISLIVVVLLILISLSACSKNDTAPNPENKEVSLENK
                     QQESKGKTNRNYLFLVRKDRKYGLYNPESGWLVEPEYEYIGPFSEERAVVKKDDRYGY
                     IDKSGRLVIDVIFDFAQDFSEGLAAVRFNGKYGYIDKDGNFVIEPQYEHAGSFSEGVA
                     KVEPPNELWSYISKDGKTVLETDYALVGDFHDGLAYVQVSYTYGYIDKRGKMVIEPKT
                     PGYTQDLPPYDFSEGFGIMKIEKEDDTIVRVFVDKNGEILGGLEFNIAYPFSEGLAYA
                     DGGYIDQTGRYVLTEADLPPDSRFSRGGKLFFELKRYEEAVKVYEKLVSRNPEDLNYR
                     YKLACAYGLNNEQEKSIEILEDINKKTPGMLY"
BASE COUNT         1236 a          548 c          765 g         1047 t
ORIGIN      
        1 atggggctgt ctgaagcttc gctgtcccca gaaaagtgag cgcagctact acttacagga
       61 tgaattaggc agtccgagaa ggctgcttgg tgaaacgggc agcacgaagg ctctctacag
      121 ctatgatgct tttggcaagc ccatcgagcc gattaatcct ttcagccaca tagccaccat
      181 agacaatatt tacagctata cgggttatca gtatgatata tccacaggct tgatgtatgc
      241 ccaggccagg tactatatgc ctgagacagg cagatttatc agcagagatc cgtataaagg
      301 gaatgttgtt gatccgcaga gccttaattt gtacttgtat tgcaagaata atcccattct
      361 atattttgat ccatccggtt atgacgcagt attagatcaa tatatctctg ataactttga
      421 tggaaagatt actgttactt ttattgtagc gcaacctatg cctggcagta gactggccat
      481 tgacgctgat ttctcgggtg atgttggaca tacgtttatt cgacttgact ttggtgatgg
      541 acgtgtaata tataaaggat tttatcctaa aaatgcccta acgcctgaac agattataaa
      601 caaagaaaat gttcctggta taatcagaga tgatagtgaa catggatggg acgtagctca
      661 agtatttgtg atagatcaaa aacaagcaga aaaaattgag aagtttatta acaattatga
      721 gaaggaatat aatatggtaa gaaataactg tactactttt gctgttgata cactgaaatc
      781 agcaggaatc aaatctccaa catcagcacg tatatggaca ttgcctgatg gagcacaaaa
      841 gctacttgtt gataatttgc cagaggaaat gtggtttaag aatattgtag cttggtggct
      901 attgcatggt ctcaaaggat actctcctgc tgatgcaggg gaagacataa gagaaaatga
      961 atgtgccata attaacacaa atggtactgt tagtccagtt agtacaaatt caggaggaaa
     1021 aaaacgatga gcaaaaaagt tattttgata gtaataatag ctattggaat tgttttagct
     1081 tttgcagttg tgagaaatgg agtattatta aaacaaagtg gtttaaacac aaaactcaaa
     1141 atcggcaagg agttatatat taatgttgcg gataacgaaa taattgatgc agattatttg
     1201 tctaaagttg ctaatggcac ttatgattca gcatttgcgc ttatggatag taaagagatg
     1261 cataagttaa tacagtattt gaaagaaaat aacttattaa ttgaacccgg taaatatgtt
     1321 attaatcaag catggtcatt tgaaaaagtt aaggatatat taagatttaa aaaaagataa
     1381 gattatggca ttcacccaaa gaattttgga atattaatgc tttttttgaa acttaataag
     1441 ttagtgaacg gtttctatct aatataagtt ccagaaataa aaatgggaat aaccgaaaca
     1501 tagcaagtgc tccctaaaac agttacaact ctcgagcaca aaatgatatg ttacctgtgg
     1561 acttcaataa ttatatccgg catgtttaaa atagaaataa ccggaattta gctggcgttt
     1621 caggtaagcg taaagtagtg catcacatag ttttcagtat gcaacgatag tacaatcaca
     1681 gtataaaagg ttgaaaaagt aatacaacgt attcaaccga aaggtctgtc aagaattttg
     1741 tgcttttaac taacactcac gattatggca ggtaattagt cacctgatct ggaaagtaaa
     1801 ttgaaagctg taagagaatt tgtccccagt tgtgaaccct gccggtccat tttttcatga
     1861 catcttgggt aactaaatac agcattttca ttaaagcatc gtcagacggg aaaatagctc
     1921 ttcgtttatt gcttttagcc atgaacatca tctccttaat ccaattatat ttggattagc
     1981 taaaagcaca atttatttta caccccctga ttttaccaat tggttttatg attatgcaaa
     2041 acaatatgga gtaaatccct actttctaat agctaaagct gttgttgaag caggtgctgg
     2101 ggataaaaat tccaaaaatc ctttattaat tggattcgaa tttgctgatg gtacagttgt
     2161 atataacttt tttggtatag gagccagcga tggcaatgcg actaaaaatg gagctaaata
     2221 tgcaaaaaat aatgggtgga caaccccaca aaaggctata gagggtggtg ccaagtttat
     2281 cggggataat tatgtgaaag ctggataaga cactttttat actgatgagg tataacttag
     2341 ccgggtataa ggaaaaaggc tatttctacc atcaatatgc ttcgaatatt gccgatcatg
     2401 tgacaaaagg aaaaagaatt gggaaaagag ctaatgactt tttagcaaac aaatatttgc
     2461 aatttaaaat accctctttt agataggaga gcgaaaaatg aagataatta tgaaaaggtc
     2521 tatttcgctg attgtggtgg tattactaat actgatttca ctgtcggcat gtagcaaaaa
     2581 tgatactgct cctaatccgg aaaacaagga agtatcatta gaaaacaagc agcaagaatc
     2641 aaaaggaaaa accaatcgga attatttgtt tctggtgcgt aaagacagaa aatacggtct
     2701 gtataatccg gaaagcggtt ggcttgtaga acctgaatat gaatatatcg gacctttttc
     2761 cgaagaaaga gctgttgtta aaaaagatga tagatatgga tatatcgata agagcggcag
     2821 gttagtaatt gatgtgattt ttgattttgc acaagatttt agtgaaggtt tggcggcggt
     2881 ccgatttaat ggaaagtatg gttatattga taaagacggc aattttgtaa ttgaaccgca
     2941 atatgaacat gccggtagtt tttccgaggg ggtagccaaa gtggaaccac caaatgaatt
     3001 atggagttat atttctaagg atggaaaaac agtgctcgaa acggattatg ctcttgtagg
     3061 agatttccat gatggtcttg cctacgttca agtatcatat acctacggtt atattgataa
     3121 aaggggtaaa atggtaattg aacccaaaac ccctggatat acccaggatt tgccgccata
     3181 tgatttttcc gaaggatttg gcataatgaa aattgaaaag gaagatgata caattgttcg
     3241 ggtatttgtg gacaaaaacg gggagattct tggcggactt gagtttaata tagcataccc
     3301 cttttcggaa gggctcgcct acgcagatgg cggatatatt gaccaaacag gacgatatgt
     3361 attaacagag gctgatttac cgccagatag ccgcttttct agaggaggaa aattattttt
     3421 tgaactaaaa agatatgagg aagcagtcaa agtttatgaa aaattggtat caaggaatcc
     3481 agaggacttg aactatagat ataaactggc atgtgcatat ggcttaaata atgaacagga
     3541 aaaatccata gaaattctgg aggatataaa taaaaagact cctgggatgc tctata
//
LOCUS       DAONUO010000213         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18042_length_3588_cov_3.472686, whole genome shotgun sequence.
ACCESSION   DAONUO010000213 DAONUO010000000
VERSION     DAONUO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18042_length_3588_cov_3.472686"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PK768_07825"
     CDS             complement(<1..503)
                     /locus_tag="PK768_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HQA60918.1"
                     /translation="MQNNKKLLRSCLRTLRILEVLSSGEEMGVTEIGKYTGLHKSTAF
                     RFLETLEHEGYVKQNPDTEKYRLSYKVLSLGFNLIEKMDVRIAARPHMEKLAIMTGET
                     VYLTIVDSGQILYIDKVNFTENLQTFHKVGDKNYVHCTASGKAQIAFFPEAECDDIFQ
                     RWGLPKQT"
     gene            861..1619
                     /gene="kduD"
                     /locus_tag="PK768_07830"
     CDS             861..1619
                     /gene="kduD"
                     /locus_tag="PK768_07830"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HQA60919.1"
                     /translation="MILDSFSLSGKVAIVTGARTGLGQGMAVGLAEAGADLVIVNRTP
                     MPETEDVIKGLGRKCVPIVADLSSLDPIPEIINRTLAEFGKIDILVNNAGIIRRAPSM
                     EFTEKDWDDVINVNLKTVFFLSQAAAREMAKNSGGKIINIASMLSFQGGILVPSYSAS
                     KGGIASLTKTLANEWSKHNINVNAIAPGYMATNNTKPIRDDEERNRSILERIPAGRWG
                     LPEDLKGAVVFLASKASDYVNGHILCVDGGWLAR"
     gene            1729..2778
                     /locus_tag="PK768_07835"
     CDS             1729..2778
                     /locus_tag="PK768_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HQA60920.1"
                     /translation="MLTSTRKFVLLLLCCVLVLALALGGCGKAPETTPADEAKEEAPE
                     ETPQETLVLRLAEVQPEDYPTTIGDKEMARIVEEKTNGRIKIEVYAGGQLGDEKSVIE
                     AVQLGGIDFARVNSQPLSEFTKSLGVFSLPYLFDNEDHFWKVMNGPIGEEILADFEEN
                     DMIGLTYYDNGSRSFYNTKREVKSPADMKGMKIRVQQSELMVKLVECLGASATPMPFG
                     EVYSALQTGVIDGAENNFPSYLTTSHYEVAKYFTLDQHTRVPEVVIASKVLWDKLSEE
                     DRAIIKEAAVASQEVQREAWKEYEKVAEEEVRAAGCIITEVDDLKEWQDAMAPLYEMY
                     QEEFGEWIERIRAAR"
     gene            2844..3326
                     /locus_tag="PK768_07840"
     CDS             2844..3326
                     /locus_tag="PK768_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HQA60921.1"
                     /translation="MFKKIDYYFNKLVEYLGMMLLLIMTVIVSWVVFSRFVLNKTPAW
                     GEEGALLCMVWFGFLCMALGVRDNRHISIEFYDKYLTPTAQKILLIIKRLLILAFSIF
                     MIVEGAKMSKVAAKNQLPGIKINSSYLYLPVVLAGISICYYILTSYLRDNHREEESDT
                     "
     gene            3323..>3588
                     /locus_tag="PK768_07845"
     CDS             3323..>3588
                     /locus_tag="PK768_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HQA60922.1"
                     /translation="MNTTAIALLLGSFAFLTIIKIPITFALGISSVITAYYLNIPAAM
                     IAQSLVRGINSFSLLSIPFFILAGEIISQGGISIQLIKFADVLVG"
BASE COUNT         1120 a          637 c          806 g         1025 t
ORIGIN      
        1 gtttgtttgg gcaagcccca tcgttgaaaa atatcatcac attccgcctc ggggaaaaat
       61 gctatttggg cctttcctga ggcagtgcag tgaacgtagt tcttgtctcc taccttatga
      121 aaagtctgga ggttttcggt gaagtttact ttgtcaatat atagaatttg accagagtct
      181 acaattgtta ggtaaactgt ctcacctgtc attatggcca atttttccat gtgagggcga
      241 gcagcaattc tgacatccat tttttcaatc agattaaagc ccaagcttag aactttatag
      301 cttagcctgt atttttcagt atcagggttt tgtttaacat atccttcatg ctctaaggtt
      361 tctaggaagc ggaatgcagt acttttatga agcccagtat attttccgat ctcagttacg
      421 cccatttctt ctcccgaaga gaggacttca agtatcctta atgttctaag acaggacctt
      481 aacaattttt tattattttg catccattac cacatccata caattctttt gcttatatgt
      541 tactttgtcc acaaagcaat gtcaatacat gctaatttat ggcagctccc aaaaaatata
      601 aaatttcgaa aaaaaaaata aaaaaaagca ggatttttaa aatttatgta gaatatatac
      661 ataaaattaa aacggtgttt tatttagtaa aacaaagcat gtattaaatt ttcatgcaag
      721 acataagtat aatataaggc acataataaa aatagtggga ataatgtttt aattactgga
      781 cgttgttgca acctgcaaat tattcttaaa ttaatgagaa atgattttgt ataaattgca
      841 aaaagacggg aggcattaaa atgatattgg atagtttcag tttatcagga aaggtagcaa
      901 ttgtaaccgg tgccagaacc ggtttgggcc agggtatggc cgtaggtttg gccgaagccg
      961 gtgcggatct ggtaatcgta aatcgcactc caatgcctga aactgaagat gtgataaaag
     1021 gcttggggag aaagtgtgtg ccgatagtag cagatctttc ttctctagat ccaattccag
     1081 aaataattaa cagaacctta gctgagtttg gtaaaataga tattcttgtc aacaatgcgg
     1141 gaattatccg ccgagcacct tcaatggagt ttaccgaaaa ggactgggat gatgttatca
     1201 atgtgaattt aaagaccgtg tttttcttga gccaggcggc agccagggaa atggcaaaaa
     1261 acagtggagg taaaataata aacattgctt caatgctctc tttccagggg ggcatactcg
     1321 tgccatctta ttctgccagt aaaggtggga tcgcttctct gaccaaaacc ttagctaatg
     1381 aatggtccaa gcacaacata aatgtcaacg ccattgcacc tggctatatg gctacaaaca
     1441 acaccaagcc tatcagggac gatgaagaga gaaacaggtc aatcctcgag cgaatacctg
     1501 caggcagatg gggtcttccg gaagacttaa aaggagccgt tgtatttttg gcttcaaagg
     1561 catctgatta tgtcaacggc catatacttt gtgttgatgg tggatggttg gccaggtaga
     1621 acttagaaag accactatta taaaaaggag gggtagcttt ttacacgaat taaagacaat
     1681 ttatcaaagt agggcgaagg tgtaaacaaa aaaaagggag gagaagttat gctgacttct
     1741 actagaaaat tcgttttatt gttgctctgt tgcgttttgg tgttagcttt ggcactgggc
     1801 gggtgcggta aagcaccgga aacgactcct gcagatgaag ctaaggaaga ggctcccgaa
     1861 gaaacgcccc aggaaactct tgtgctgaga ctggcagaag tacaacctga ggattacccc
     1921 acgacaatcg gagacaaaga aatggcaagg atagttgaag aaaagacaaa tggtaggata
     1981 aaaattgagg tatatgccgg tggacaattg ggcgatgaaa agagcgtaat tgaggctgta
     2041 cagctgggcg gtattgattt tgcaagagtc aattctcaac ccctgtctga gtttacaaag
     2101 tcactcggtg tattttccct tccatattta tttgacaatg aggatcattt ctggaaagtt
     2161 atgaacggac caattggaga agaaattttg gcggatttcg aagaaaacga catgatcggt
     2221 ctgacctact atgacaacgg ttcaaggagt ttttataaca cgaagcgtga agtaaagtcc
     2281 ccggctgaca tgaaaggcat gaaaataagg gtgcagcagt cagagcttat ggttaaactt
     2341 gtagagtgct taggagcatc agcgacccca atgcctttcg gcgaagttta cagtgcatta
     2401 caaacgggcg taattgacgg tgcggaaaac aacttcccca gctatttgac aacttctcat
     2461 tatgaagtag caaaatactt taccctagac caacatacta gagttccgga agtagtaata
     2521 gcctctaaag tactgtggga taaactaagc gaagaagatc gggctatcat aaaagaagca
     2581 gctgtagctt ctcaggaagt ccagagagag gcatggaaag aatatgaaaa agttgccgaa
     2641 gaagaagtca gggcggctgg ttgcataata actgaagttg atgatctaaa agaatggcag
     2701 gatgccatgg cgccccttta cgaaatgtat caagaagaat ttggagaatg gattgaaagg
     2761 atcagagctg ctcgctgata actcttgctg gttatgccgc cggtcttgaa tcggcggcat
     2821 aacaatagtt tggaggggag aatttgttta aaaagatcga ctactatttt aacaaacttg
     2881 tagaatatct aggtatgatg ctattgctga taatgacggt tatagtgtcc tgggtggtgt
     2941 tctccagatt tgtcctcaac aaaactccag cctggggcga agagggtgca cttttatgta
     3001 tggtatggtt tggtttctta tgtatggcct taggagtaag agacaacagg catatttcca
     3061 tcgagtttta tgataaatat cttaccccaa cagcccaaaa aatactatta attatcaaaa
     3121 ggcttttaat attagctttt tctattttca tgattgttga aggagcaaaa atgagcaagg
     3181 tggcagcaaa aaatcaactg ccgggtataa aaatcaattc atcctatctt tatttgccgg
     3241 tggttttagc aggaatatcg atttgttact acatactaac atcatatttg agggataacc
     3301 acagggagga ggaaagtgat acatgaatac tacggcaatt gcattactac tgggcagctt
     3361 tgccttttta acgattatta aaatacccat tacttttgct cttggcattt cttcagttat
     3421 aactgcctat tatttaaata tacctgcagc catgatagcc cagtctcttg tcaggggaat
     3481 aaattccttc tctctgctga gtatcccctt tttcatatta gccggggaaa ttatcagtca
     3541 gggcggcatt tctatacagc taatcaagtt tgctgatgtt ttagtggg
//
LOCUS       DAONUO010000214         3577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18100_length_3577_cov_3.201874, whole genome shotgun sequence.
ACCESSION   DAONUO010000214 DAONUO010000000
VERSION     DAONUO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18100_length_3577_cov_3.201874"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..555)
                     /locus_tag="PK768_07850"
     CDS             complement(91..555)
                     /locus_tag="PK768_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60923.1"
                     /translation="MKSFIAAEALLITVLIIILIVHYYCGKNLSNSAAEKLPRLETLI
                     FIKNAEDVIEGIVKDFYSKQEMTSELLIVDCGSSDQTHRILERLARRYLGMKFILLSD
                     LPLNSCIQEALKYTSSPALLLIDCKSMSSKEILKSLALVSKNIRIGLKAYEK"
     gene            complement(624..>1160)
                     /locus_tag="PK768_07855"
     CDS             complement(624..>1160)
                     /locus_tag="PK768_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA60924.1"
                     /translation="GEEAIQVVMDKKPQVLLLDINMPKLNGIDVTKALKNKGVDTKIV
                     ILTIYDDREYLLELIKQGISGYILKDIEPSGLIDAVRCASQGETYIQPNLTKALVAEY
                     NRLTQPVSNQGTRKDLTVREKEVVKYIAEGMSNGEISQKLGISDKTVKNHVSSILRKL
                     NLMDRTQVAVYAIKNKLV"
     assembly_gap    1161..1170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1171..1259)
                     /locus_tag="PK768_07860"
     CDS             complement(<1171..1259)
                     /locus_tag="PK768_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HQA60925.1"
                     /translation="MLADDHVLMRKGLRKILEMEEDIEVIGEAS"
     gene            complement(1298..2449)
                     /locus_tag="PK768_07865"
     CDS             complement(1298..2449)
                     /locus_tag="PK768_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQA60926.1"
                     /translation="MINKGRLNINYLNEVIKNTIEAIETGQKEIFNIFEHARQECERL
                     EKELEKLRGQVKATIESVDRLVKLEKAAKFKLMTVSRDFEKYTEEDIRAAYEETKEIQ
                     IQLSLERQKELQLREKRNDLERSWKSMQNILKQSEHLMMQLGVALGFLKSNLEDVSGH
                     ISDMNEKRKLAARVIKAQEEERRRVARDIHDGPTQTLANIIIQTEICERLLQKDLAEA
                     RKELNELKYIVRQSLQELRKIIFNLRPSSLDDLGLQAVAKRFCEEFEEDTGIKTEFHF
                     FGDYRRLNSDIEVTLFRVIQEALINVRKHSKARTCLVKLEYGNERINLVVADDGIGFE
                     TGVIEGKSDERHFGLMTIKERVDLISGSFNIESAPGQGTKIYVSVPFDI"
     gene            complement(2479..>3577)
                     /gene="metK"
                     /locus_tag="PK768_07870"
     CDS             complement(2479..>3577)
                     /gene="metK"
                     /locus_tag="PK768_07870"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQA60927.1"
                     /translation="AKDPSARVACEVAVTTGLVLVMGEITTDCYVDIPYITRETIREI
                     GYTRAKYGLDCDTCAVITTINEQSPDIAMGVNEAYEVRDTSNEVDEVDKIGAGDQGMM
                     FGYAVNETPELMPLPISLAHKLARRLAQVRKTRVLPFLRPDGKTQVTVQYEDSKPVRV
                     DKVVVSAQHKPSTDLLTLKEGIIEEVVKKVIPEQFLDKNTQFFVNPTGRFVIGGPHGD
                     SGLTGRKIIVDTYGGFGRHGGGALSGKDPTKVDRSGAYAARYVAKNIIAAGLASRCEV
                     QVAYAIGVAKPVSIMVDTFGTGILPDEKIEKLVRNNFDLRPGAIIRDLDLLRPIYKLV
                     SCYGHFGREELALPWERVDKAEILKNQASSL"
BASE COUNT          865 a          885 c          670 g         1147 t
ORIGIN      
        1 ttggtagggt ccaggccttt ttttcttggt ttttactgta aaagtcaggt tatacgacaa
       61 aatagtaaaa cttggaataa ctcacttgtc ctatttttcg taggctttta gacctattct
      121 gatatttttt gacaccaggg ccaaagattt aagaatttcc tttgagctca tgcttttgca
      181 gtcaatgagc agtagagccg gtgaacttgt atattttaat gcctcctgta tgcatgaatt
      241 aagcggcaaa tctgacaata aaataaactt catgcctaaa taccgcctgg ccagtctttc
      301 aagtatccga tgggtttgat cgctggaacc acagtcaact atcaagagct ccgaagtcat
      361 ttcttgcttg ctgtagaaat cttttacaat gccttctatt acatcttccg catttttaat
      421 aaagataagg gtttccaatc ttggcaactt ttcagcagca ctattcgaca agtttttgcc
      481 gcaataataa tgtacgatta agatgattat taagaccgta attagaagtg cttcggcagc
      541 tatgaagctt ttcaatcata tcccccccaa gtcttgcaaa tatatattca atctatgcta
      601 ttcgggagga tatgcatttg tgactaaact agcttatttt ttattgcata cactgctact
      661 tgagtcctgt ccatcaggtt aagcttgcgc aaaatgctgc tgacatggtt ttttaccgtt
      721 ttatcactta tgcccagctt ctgggaaatt tcgccattgc tcataccctc ggctatgtat
      781 ttaaccactt ctttttctct gacggtaagg tctttcctcg ttccctgatt ggatacgggc
      841 tgtgtcaacc gattgtattc ggcaaccagg gccttagtta gattgggctg tatataagtt
      901 tctccctggc tggcacacct tactgcatca atgagaccgc tgggctctat gtcctttaat
      961 atgtatcccg atattccctg ctttatcaac tccaaaagat attccctgtc atcgtaaatg
     1021 gtcaatatta ctatttttgt atcaacgcct ttattcttta atgcttttgt aacgtcaatg
     1081 ccgtttagtt taggcatgtt aatatctaga agaaggacct ggggtttttt gtccattacc
     1141 acctggatgg cttcttcccc nnnnnnnnnn gaagcctctc ctatcacttc aatgtcctct
     1201 tccatttcaa ggatttttct aagccccttt ctcatcagca catggtcatc ggccaacacg
     1261 actgatattt tctccaaact gatcctcctt tccaaatcta tatatcaaaa ggaacactga
     1321 cgtagatttt tgtaccctgg ccgggcgccg attcaatatt aaaagacccg cttatcaaat
     1381 ccaccctttc ctttatcgtc attaagccaa aatgtctttc atcgcttttg ccctctatta
     1441 caccggtttc aaaaccaatc ccgtcatcgg ctaccaccag attaattctc tcatttccgt
     1501 attcaagctt tacgaggcag gttcttgcct ttgaatgctt gcgcacattt atcaaagctt
     1561 cctggataac tctaaaaagt gtcacctcta tatcagaatt gagtcttcta taatccccga
     1621 agaaatgaaa ttcggtctta atgcccgtgt cttcctcaaa ttcttcacaa aagcgctttg
     1681 ccaccgcctg caggcccagg tcatcaaggg aagatggcct taaattaaag ataatctttc
     1741 tgagttcctg cagggattgt ctcacaatgt acttcagttc attgagctct tttcttgctt
     1801 cggccaagtc tttttgcagc agtctctcgc aaatttccgt ttgaataatg atgtttgcca
     1861 gtgtctgggt tgggccatcg tgaatatcac gggcaacacg ccgccgttcc tcttcttggg
     1921 cctttattac tctggctgca agcttgcgct tctcgttcat gtccgatatg tgcccagaca
     1981 cgtcctcgag attgctcttt aaaaagccca aggccacacc caactgcatc atcaagtgtt
     2041 ccgattgttt gagaatgttc tgcatgcttt tccaactgcg ctctaaatca ttccgctttt
     2101 ctctgagctg aagttccttc tggcgctcca gggaaagctg aatctgtatt tcttttgtct
     2161 cttcatatgc cgcccttata tcttcttcag tatacttctc aaagtcccga cttacagtca
     2221 tcagcttgaa ctttgctgct ttctctagct ttacgagcct gtccacgctt tctatggtag
     2281 ccttcacctg gcccctcagt ttttcaagtt ctttttctaa tcgctcacat tcctgccttg
     2341 catgctcaaa aatattaaag atttcctttt gcccagtttc tatagcctcg atggtatttt
     2401 ttataacctc attcaaataa ttaatgttca agcgtccttt gttaatcata aaaacccccg
     2461 gttaaaagga gcagcctact agaggctgct agcttgattt tttaaaattt cagctttgtc
     2521 tactctctcc cagggcagtg ccaattcttc tcgtccaaag tggccatagc aggataccaa
     2581 tttgtagatt ggcctaagta agtctaagtc ccgaattata gctcccgggc gaagatcaaa
     2641 attgttcctt acgagctttt ctattttttc atcaggcaaa atgccggttc caaaggtatc
     2701 aaccattatg gaaacgggtt tggctacacc gatagcgtat gccacctgca cttcacatct
     2761 tgaagcaagc cctgctgcaa tgatattttt agccacatag cgtgcagcat aagctcccga
     2821 gcggtctact ttggtcggat ccttgccgga tagggcacct cccccatgcc ggccaaatcc
     2881 accgtaagta tcgacgatga tcttgcggcc tgtaagaccc gagtccccgt gggggccacc
     2941 tattacaaat ctgccggtgg gatttacgaa aaactgggta tttttatcca agaattgttc
     3001 cggaattacc ttttttacaa cctcttctat aataccctcc tttaatgtta gaaggtcagt
     3061 tgaaggttta tgctgggcag atactaccac cttgtccact ctcacgggct ttgaatcctc
     3121 atactggacc gtaacctggg tttttccgtc cggcctcaaa aagggaagca cacgggtttt
     3181 cctgacctgc gccagccttc tggccagctt atgggcaagg gaaatcggaa gaggcatcag
     3241 ttcaggtgtc tcgtttacag catatccaaa catcatgccc tggtcgccgg caccaatttt
     3301 atcgacttca tctacttcgt tggaagtatc cctgacttca taggcttcgt tgacacccat
     3361 ggcaatatca ggggattgtt cgtttattgt agtgattact gcacaggtat cgcaatcaag
     3421 gccgtattta gcgcgggtat agccaatttc ccttatagtc tccctggtaa tgtatggaat
     3481 gtccacatag caatcggtgg taatttcacc cataaccagc accaaacctg tagtgactgc
     3541 aacttcacag gcaaccctgg cggaaggatc cttggct
//
LOCUS       DAONUO010000215         3564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18181_length_3564_cov_3.333143, whole genome shotgun sequence.
ACCESSION   DAONUO010000215 DAONUO010000000
VERSION     DAONUO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18181_length_3564_cov_3.333143"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK768_07875"
     CDS             <1..469
                     /locus_tag="PK768_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HQA60928.1"
                     /translation="SGRETIEQVVKNILPFIAFVATLIGIILKSGIGNWIANIISPMA
                     GTLPGLLAISVVCAIPILSPLLGPGAVIAQVVGVLVGVEIGKGNIPPQFALPALFAIN
                     PQVGCDFIPVGLSLGEAEPETVEVGVGAILISRLITGPISVLIAYLFSIGLYT"
     gene            543..908
                     /locus_tag="PK768_07880"
     CDS             543..908
                     /locus_tag="PK768_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HQA60929.1"
                     /translation="MDKYKAKVLSIGEMVQDLLEQGIVVLFDSTAPKELQEIAVVHTG
                     GTLMADVAQGDSIILGDSAYTVTAVGEIANKNLKIIGHVCLKFDARSSPELPGDIHLK
                     GEQLPQFAVGDVITIKAAV"
     gene            1004..2293
                     /locus_tag="PK768_07885"
     CDS             1004..2293
                     /locus_tag="PK768_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQA60930.1"
                     /translation="MLNLNTKLRELDEQGKKIKVGIVGAGQMGRGMVSQMFCMKGIAP
                     AIISDLDVERARRAYELAGVKTEDIAFASKPSEAEQNIQKGKYVITDNLDVVTNSVPV
                     DVVIDATGVPETGARIAFDSIYNGKHIVMLNVETDVTIGPILKKMADSAGVVYTVSAG
                     DEPGAIKELYDFVDAMGFEILVVGKGKNNPLNVEATPDTAKDRALAYNMNPKMLASFI
                     DGTKTMVEMTCVSNATGFLPTRKGLIGPKATVKDLPKLLSLKEQGGILDSYKVVEYVD
                     GVAPGVFVIVTTDLPAVKEEMAYLSMGEGPNYVIYRPYHLTSIETPLSAARAYIYREP
                     TIAPMGAPVSETVAVAKRVLRAGEYLDGIGGYTVYGVIDTYANAKKDNALPLGLVSSK
                     VKLIKDVKKGETLTYDMVELDEESLILQLRRLQDKVI"
     gene            2415..2600
                     /locus_tag="PK768_07890"
     CDS             2415..2600
                     /locus_tag="PK768_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60931.1"
                     /translation="MADGEISGSGIEIAGQVKISISKAEGFKLKCPCSRNGKRIDADI
                     KWRKPGKSNTQGRIFNL"
     gene            2784..>3564
                     /locus_tag="PK768_07895"
     CDS             2784..>3564
                     /locus_tag="PK768_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HQA60932.1"
                     /translation="MTLTREKRLWTYQKMLEIRNFELEVDRLFKANMIWGTCHLSVGQ
                     EASAVGSIAALEERDMITSTHRGHGHCIAKGGRLPQMFAELLGKETGYCRGRGGSMHI
                     ADLELGNLGANGIVGAGIPIATGAALASKLKKDGKVTLCFFGDGATSQGAFHEAVNLA
                     SVMKLPVVYVCENNLYGMSVPFRKASAVENAADRAKAYNIPGIVVDGNDVEAIYEVVK
                     EAVERARRGEGPSLIEAKTYRWLGHSKSDPRVYRTREEEEEW"
BASE COUNT         1100 a          675 c          907 g          882 t
ORIGIN      
        1 atccggccga gaaacaattg aacaggtcgt aaaaaacatc cttccattta tagcttttgt
       61 tgctaccctg ataggtatca tattgaaatc cggtatcggt aattggatag caaacataat
      121 atcaccaatg gctgggactt taccgggctt gcttgctatt tccgtcgtat gtgcgatccc
      181 catactttca cccttactgg ggcccggagc cgtaatcgca caggttgtag gtgttctggt
      241 gggtgtggaa ataggcaagg gcaacatacc gccacaattt gctctaccgg cactgtttgc
      301 cattaatccc caagtaggat gtgactttat ccctgtagga ttgagtctgg gagaagcgga
      361 gccggaaact gttgaagttg gcgtgggtgc catacttata tcccgattga taacgggacc
      421 gatttcagta ctaattgcct acctgtttag catcggatta tatacctaat acgaaaagta
      481 ttctgccgca atgatattgc ggcagaatac ttttaccaaa tattactcaa aggagtgagc
      541 ccatggacaa atacaaggca aaggttttat caatagggga gatggtacaa gatttgctgg
      601 agcagggcat agtggtattg tttgacagca cagcgccaaa agaactgcaa gaaatagcag
      661 ttgtacatac gggaggaact ctcatggcag atgtggcaca aggtgattct ataattcttg
      721 gtgattcagc atatacagtt acggctgttg gggaaattgc aaacaaaaac ttaaaaatta
      781 tcgggcatgt ttgccttaaa tttgatgcta gaagttcgcc agaattgccg ggggatatac
      841 acttaaaggg tgaacagttg ccacaatttg cagtgggaga tgtgattaca ataaaagctg
      901 ctgtataata tcctttacta aatcttctag agatttgtca tttatgtgtt tgaaaatcat
      961 aggttgaatt taaacaataa cttattgagg gagatgttgt attatgctta atttgaatac
     1021 caaactaagg gaacttgatg agcaaggcaa aaaaatcaaa gtcggtattg tcggggcagg
     1081 gcaaatggga cgtggtatgg taagccagat gttctgcatg aagggcattg ctcctgctat
     1141 aatatccgac ttagacgtgg aaagggctag aagggcttac gaacttgccg gtgtaaaaac
     1201 cgaggatatt gcttttgcaa gcaaaccttc agaagccgag cagaatatac aaaaaggtaa
     1261 gtatgttatt accgataatc tcgatgtggt aaccaactct gtgcccgttg acgtagttat
     1321 cgatgccact ggggtaccgg aaaccggtgc caggattgct tttgattcaa tttacaatgg
     1381 caagcatatt gtaatgctca atgtagagac cgatgtaacc ataggcccca tactaaaaaa
     1441 gatggccgac agtgcaggag tagtgtatac ggtgtctgca ggcgatgaac cgggtgccat
     1501 aaaagaattg tacgattttg ttgacgccat gggatttgaa atattggtgg taggtaaagg
     1561 caagaacaat ccgctgaacg tagaagctac gcccgatacg gcaaaggaca gggctctagc
     1621 atacaatatg aacccgaaaa tgctagcttc ttttatcgat ggaactaaga caatggttga
     1681 aatgacctgt gtctccaatg cgactggatt tttgccaacc agaaagggct tgatagggcc
     1741 gaaggcaacg gtaaaagatt tgccaaaact gttaagttta aaggaacagg gaggcatttt
     1801 agacagctat aaagtagtag aatacgtaga cggagtggcg ccgggagttt ttgtaatagt
     1861 aactaccgat cttcctgcag tgaaggaaga gatggcttac ctttccatgg gtgaagggcc
     1921 aaactatgtg atttacagac cctatcacct aaccagtatt gaaacaccct tatccgcagc
     1981 ccgggcatat atataccgtg agccaaccat tgcgccaatg ggagcaccgg tgtcagagac
     2041 cgttgcagta gcaaaaaggg ttctaagagc aggtgagtat ttggacggca taggaggata
     2101 caccgtatac ggcgttatag atacctacgc caatgccaaa aaggacaacg ctttgccatt
     2161 aggtcttgta agttctaaag taaaactgat taaggatgta aaaaaaggag aaaccctgac
     2221 ttacgacatg gttgaattgg atgaagaatc actgatactg cagcttcgcc gattgcagga
     2281 taaggttatt taatgacgac aaaaccttta ctcaacaaaa tatgccaaca catggcacaa
     2341 aatttgccta taaaaatata ggcgccatcg aaattaagga tatcagaaac tttgcctttt
     2401 ctcttcacgc atgtatggcc gatggggaaa taagcggctc gggaatagaa atagcggggc
     2461 aggtgaagat tagcatatct aaagctgaag gatttaaact gaagtgtccc tgtagtagaa
     2521 acggaaaaag aattgatgct gatatcaagt ggcgaaagcc tggaaaaagc aatacacaag
     2581 ggcgtatttt taacctgtaa gttacttcaa gaacatctgg atatcaattc tcccgatgca
     2641 tataggctgg ccagtataag ctgtgatgtg aggatttgtc agttggtaaa tccacaagtt
     2701 accgtcagag tttgccttcc taaatatctg ttgaagatta agcagtaaga tttgcttatc
     2761 aaaaattaaa aggagatggt ttaatgactc tcactagaga gaagagactg tggacgtatc
     2821 aaaaaatgct cgaaataaga aattttgagc tagaagtaga caggctgttt aaggcaaata
     2881 tgatttgggg gacctgccat ttgtccgtcg ggcaggaagc ctccgctgta ggttcaatag
     2941 cagccctaga agaaagagac atgataacca gcacccaccg tggccacggt cactgcattg
     3001 ccaaaggggg cagacttccc caaatgttcg ctgagctcct cgggaaagag accggttact
     3061 gcagaggcag gggagggtca atgcacattg cagacctgga attgggcaac ttgggcgcta
     3121 acggtatcgt tggtgccggt atacccattg caaccggggc agccttagcg tcgaaactca
     3181 aaaaggatgg aaaggtgacc ttgtgctttt tcggagatgg agccacaagc cagggggctt
     3241 ttcacgaagc cgtaaacctg gcatcagtca tgaaattgcc ggtagtttat gtctgtgaga
     3301 acaatcttta cggcatgtcg gtgcccttta gaaaggccag tgccgtagaa aacgcggcag
     3361 atagggcaaa ggcatataat attcccggaa tcgtagttga cggcaatgat gttgaagcca
     3421 tatacgaagt ggtaaaggaa gctgttgaaa gggctagaag aggagaaggt ccttccctta
     3481 tagaagcaaa aacctatcgc tggctcggcc attcaaagag cgacccgagg gtttaccgga
     3541 caagagagga agaagaagag tgga
//
LOCUS       DAONUO010000216         3554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18255_length_3554_cov_3.449843, whole genome shotgun sequence.
ACCESSION   DAONUO010000216 DAONUO010000000
VERSION     DAONUO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18255_length_3554_cov_3.449843"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PK768_07900"
     CDS             <1..303
                     /locus_tag="PK768_07900"
                     /inference="COORDINATES: protein motif:HMM:NF037153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60933.1"
                     /translation="DELFSGEGRYYMNLSTVYPGEEELDDIEYYELVERSGYIYSKFG
                     RPYRKKRVRLLKEGSIFSRKIEGRIIDIRPDVFDEHSVYLNGKAFLIPLGRCNDES"
     gene            293..1351
                     /gene="csm5"
                     /locus_tag="PK768_07905"
     CDS             293..1351
                     /gene="csm5"
                     /locus_tag="PK768_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HQA60934.1"
                     /translation="MNLSVKLTTLTPVNIGSGEILSQFCDYVYDKGFIYYLDYDLIAQ
                     ELAKMPEGDRLIDEFVAIVRTQAGGNVQNRFRIKGFLESTGLDFQNFASKQIAVEDEI
                     KEQIQLQVKTGGRPYVPGSSIKGAIRTAIVSNFFTAELEKNIKNTKEYIGQDILGKYD
                     EDILKFLQVSDTMPFNEEDLGIVRFLRYNLKTGDLDIPVVKEVILSERESSFKISFKA
                     KKGQVPSRFVFLEEGKGEQIFEIINKYSRENVENEIKELNRNKSGELKDVKEFYEELL
                     DTVSTADVSKEAYMRIGSGKTFYDNTVAHKLSQAFLKGIIKQNYRKADPNFFPKTRTV
                     VLHEGKKTAPGWVKLEKV"
     gene            1375..2790
                     /gene="csx2"
                     /locus_tag="PK768_07910"
     CDS             1375..2790
                     /gene="csx2"
                     /locus_tag="PK768_07910"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HQA60935.1"
                     /translation="MAKILIATLGTGQLKDDDTSIREYREAVYRFLDSGKTYKTSFIA
                     AALSDHLQVDKLYLIGTSKSMWEEVYRYFSTASEQSVDDDYWVELHERIKDLNPRDEK
                     LTEKELSRVNEAIDDYLKHIQPEATGGSRCFIIDYGLDEREIWNNFDVMMQIGETLTE
                     DDEIYLDITHAFRSIPLFLYIMLDLIRILKLKRDFKLGGLYYGMLDVIREMGYAPIVD
                     LSPLYNMTLWTRGAYNFLNFGNGYLLADLVSNQELSDRIMNISDIVNINFIDEFKREI
                     DRLAGLLNDANSKDPLIKYMEPYLMTFVDRFKGINSSSQLQLTLAKWYFEHKRFAQCY
                     ICLAEAIVSRILEEYRARDSKIKWNNANRNKIKYLIYKTNFDTMEKFKDLCDLYREIS
                     TTRNLIAHAGFADEKELYKDKAHKNFTSNRQKSVATKIDYKDAINKINSHMRNTENHV
                     FNNKHLKEIPDLFPFNKLSGN"
     gene            3041..3430
                     /gene="csx20"
                     /locus_tag="PK768_07915"
     CDS             3041..3430
                     /gene="csx20"
                     /locus_tag="PK768_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx20"
                     /protein_id="HQA60936.1"
                     /translation="MRQVFLLFSHQLTPAQMDELENKYKVDKIVHLPAGLQELWSNIP
                     PELPTIKDYLKDVLIWLKENSNPGDLVLVQGEFGAVYIVVNFCIKEGLVPIYATTKRE
                     ASEEILYDGTVQVSRKFSHVRFREFEM"
BASE COUNT         1137 a          673 c          829 g          915 t
ORIGIN      
        1 gatgaattgt tttctgggga aggcaggtac tacatgaacc tttccacggt atatcccggc
       61 gaggaggagc tggatgatat agagtactac gagctggtgg aaaggagcgg ttacatttac
      121 tccaagttcg gaaggcccta tagaaagaag cgggtaaggc tacttaagga aggcagcatt
      181 ttttcaagaa aaattgaggg caggatcatc gacattcgcc ctgatgtctt tgatgagcac
      241 agtgtgtatt tgaacggaaa ggcttttctc atccccttgg ggaggtgcaa tgatgaatct
      301 tagtgttaaa ctcacaaccc tgacgcccgt caacatagga agcggagaga ttttgtcaca
      361 gttttgtgat tatgtgtatg acaagggatt tatctactat ctggactacg atttgatcgc
      421 ccaggagctt gcgaaaatgc ctgaaggcga tagacttatc gatgagtttg tggccattgt
      481 ccggacccag gccggtggga acgtgcagaa caggtttaga ataaagggct ttttagaaag
      541 tacggggcta gattttcaaa actttgcttc aaagcagata gctgtggaag atgaaatcaa
      601 ggaacagatc cagctccagg taaagaccgg tggcagaccc tacgtgccgg gcagcagtat
      661 aaagggggcc atccgcaccg ccatcgtcag caattttttc acggcagaac tggagaagaa
      721 tatcaaaaac accaaggagt acatagggca ggacatcttg gggaaatacg atgaggacat
      781 attgaagttc ctgcaggtat ctgacacgat gccttttaat gaagaggacc tggggattgt
      841 aagattttta aggtacaact taaagaccgg cgacttggat attcccgttg taaaggaagt
      901 cattctttcg gaaagggaga gcagctttaa aatcagcttt aaagcgaaaa aaggtcaggt
      961 acccagcaga tttgtatttc tggaagaagg aaaaggggag cagatttttg aaattataaa
     1021 taagtactcg agggaaaatg tggaaaacga gataaaggag cttaatagaa acaaaagcgg
     1081 cgaattgaaa gatgtgaagg agttttatga agagctcctt gatacagtca gcaccgctga
     1141 tgtcagcaaa gaagcctaca tgagaatagg ttccggcaag accttctatg acaataccgt
     1201 tgcacacaaa ctgtcacaag catttttaaa ggggataata aaacaaaact atagaaaggc
     1261 agatccaaat ttcttcccta aaactaggac cgtagtgctg catgaaggca aaaaaacggc
     1321 acccggttgg gtcaagctgg aaaaagtata gaagctttgg agggggaagg tagagtggcg
     1381 aaaattttga ttgcaacctt gggtacaggt caattgaaag acgatgacac ctctataagg
     1441 gaatacaggg aagcagttta cagattcctg gacagcggca aaacctataa aacttccttt
     1501 atagcagcgg ccttgagtga tcatttgcaa gtcgacaaat tgtatttgat aggcacctca
     1561 aaatccatgt gggaagaggt ctacaggtat ttttccactg ccagcgagca aagcgtcgat
     1621 gatgactatt gggttgaatt gcacgaaagg ataaaggatc ttaatcctag agatgaaaaa
     1681 cttacggaaa aagagctgtc tagagttaac gaggccattg acgattattt gaaacatatt
     1741 caacccgagg ccacgggagg ttcccgctgc ttcataatcg actatggatt ggacgaaaga
     1801 gaaatctgga ataactttga tgtgatgatg cagatcggcg agaccctgac tgaagatgat
     1861 gagatttacc tggatataac ccatgctttt cgctcaatac cgctgttcct ctacataatg
     1921 ctggacctca taagaatact caagctcaaa agggacttta aacttggcgg cttgtattac
     1981 ggcatgttgg atgtgattag ggaaatgggc tatgctccca tagtggactt gagccccctt
     2041 tacaacatga ctctgtggac caggggagca tataactttt taaacttcgg aaacggctat
     2101 ctcctggcag atttagttag caatcaggaa ctatccgata gaatcatgaa catttcggat
     2161 atcgtcaaca ttaactttat tgacgaattt aaaagggaga ttgaccgcct ggccggcttg
     2221 ctaaatgatg caaattcaaa agacccctta atcaaataca tggaacccta tttgatgacc
     2281 tttgtggacc ggttcaaagg gattaattcc agttcgcagc tgcaattgac gctggcaaaa
     2341 tggtacttcg agcacaagag attcgcccag tgctatatct gcctggcgga ggccattgtt
     2401 tcaagaatac tggaagagta ccgggccagg gattccaaga tcaagtggaa caacgccaat
     2461 agaaacaaaa tcaagtattt gatatacaag accaactttg acaccatgga aaaattcaaa
     2521 gacctgtgtg atctatatag agaaatcagc acaactagaa atctcatcgc ccatgcaggt
     2581 tttgcagacg aaaaagagtt atataaagat aaagcacata aaaactttac gtctaatcgg
     2641 caaaagtctg ttgctactaa aatagattat aaagatgcaa taaataaaat caattcacac
     2701 atgcgcaaca cggaaaacca tgtgtttaac aataagcatt taaaagagat acccgactta
     2761 ttccccttta ataaattatc cggcaactaa ttggatctct ttttctcaaa agttcacccc
     2821 atcagcttga aacgaattag aaaagtcatc gaccgaaaac acgaaaaatt tgatgcgtcg
     2881 atgacttttt tgtttcacac ggccgaagct ttgtgtatca aggggaatgt ggtataatat
     2941 agaagattgc tgaaagcctc ttcatgtaca aaattatcat ggtatgcttt cagtctaccc
     3001 ttcaggggtt caagctgctt ttatatggag gtcaaatttt gtgcgacaag tatttttact
     3061 tttttctcac cagcttactc cagctcaaat ggatgaattg gaaaacaaat ataaagtaga
     3121 taaaatcgtc caccttcccg cagggctgca ggagctctgg tcaaacatac cgcctgaact
     3181 tcctacaata aaagactacc ttaaagatgt actcatctgg ctaaaagaaa actcaaatcc
     3241 gggggacctg gtccttgtcc agggagaatt cggagcggtt tatatcgtag ttaacttttg
     3301 cataaaagag ggcctagtcc caatatatgc cactacaaaa agagaggctt ctgaagaaat
     3361 cttatatgac ggaaccgtcc aggtgagcag gaagttttcc catgtgagat ttagggaatt
     3421 tgagatgtga taccgatttg gttgctttta gtccacctat aaggttttga aacagtgcac
     3481 cgatcatgtt atcagtcgtt aacggatttt tttgaaacgc tgttgctttg atttggcctt
     3541 tcttaatatt gttc
//
LOCUS       DAONUO010000217         3553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18261_length_3553_cov_4.181818, whole genome shotgun sequence.
ACCESSION   DAONUO010000217 DAONUO010000000
VERSION     DAONUO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18261_length_3553_cov_4.181818"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK768_07920"
     CDS             complement(<1..246)
                     /locus_tag="PK768_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60937.1"
                     /translation="MLTINRNGESTKIKKRIFIGLLAASVLLIFGIILSGVLIYYNKF
                     GAWYRYLLLAIIALLGLFILIVGIGLAGVVMTLLNVRK"
     gene            complement(230..1171)
                     /gene="fmt"
                     /locus_tag="PK768_07925"
     CDS             complement(230..1171)
                     /gene="fmt"
                     /locus_tag="PK768_07925"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQA60938.1"
                     /translation="MRVVFMGTPEFAVASLDILLKERIDIVAVVTQPDRPVGRKRIIT
                     PPPVKKLAIEHNIPVYQPAKVRDEEFIKKLLSLEPDLIVVVAFGQILSRQVLDIPKIG
                     CINVHASLLPKYRGAAPVQWAIINGEKVTGVTTMWMDEGLDTGDIFLQESIEIKDDWT
                     ALELFSELSILGGKLLIDTLKHIKSGNIIRIAQDDTQATYAPMLKKEIGKIDWSKSAN
                     EICNLIRGTYPWPCAYTILKGREIKIFKARPFPSGNAPAPGRYRGLVKNEGFLVGTGT
                     GDILVTELQEAGSKRMKATEYLIGHHFKEGDLFADYQ"
     gene            complement(1174..1638)
                     /gene="def"
                     /locus_tag="PK768_07930"
     CDS             complement(1174..1638)
                     /gene="def"
                     /locus_tag="PK768_07930"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQA60939.1"
                     /translation="MALRSIREIGDDILRKKSKKVEHFNERLHTLIDDMLETMKEADG
                     VGLAAPQVGILKRVVVIDVGDGPIELVNPEIIETEGEDSQPEGCLSVPGVLGEVPRPK
                     RVKLRAQDRWGKTFEIEGEDLLARAFCHEIDHLDGILFIDKVTKFLEPDSEG"
     gene            complement(1650..3491)
                     /gene="priA"
                     /locus_tag="PK768_07935"
     CDS             complement(1650..3491)
                     /gene="priA"
                     /locus_tag="PK768_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQA60940.1"
                     /translation="MKKRKISISFAKNYEDVYEFLIENGADTAQNLSKKLDIPLASVR
                     SALTSLDKKGIVKKEYRAVRRMPMAFSSEKYHNRISLTDEQHDAVETISDIFSRSKKT
                     TLIYGVTGSGKTEVYIRVIQRVLSQGESVLVLVPEISLTPQMLSIFRQRFPGKTAVIH
                     SRLSPGERFDEWQRIHSGSATVVIGARSAIFAPVQNLGLIIIDEEHEASYKNGEHPYY
                     DARLVARFRSKLENAMLIMGSATPSVESFYRAAKGDYSLVKLTRRVSGRVLPKMEIID
                     MISELKSGNRHIFSRKLLSSIKATLDANKQVILFLNRRGHSTFVICRDCGFVLKCKYC
                     DISLTYHFNDKNAKCHYCGYTVKAPDFCPVCRGQNIRYFGAGTEKVEQEVKKFFPACK
                     TLRIDSDSTSKKGSLEKMLLSFKRGAAQILIGTQTVAKGLDFPNVTLVGIVSADTALN
                     MPDFRCGERTFQLITQVAGRAGRGDTPGRVYVQTYAPEAYAIKAACSMDISAFYKEEL
                     KMRHKALYPPFCHMLIIRFKGHNEDVVKKEAEDVKKVLSERYNNEVKMYGPVPAARSK
                     IKENYRYNILLKSKQVRVLSEICNHMQKTCINRHVDIAWDLEPLDLL"
BASE COUNT          942 a          826 c          667 g         1118 t
ORIGIN      
        1 tttcctaaca tttaagaggg tcattacgac tcctgcaagg ccgatgccga caattaagat
       61 aaataggcca aggagtgcaa ttatagcgag caataaatag cggtaccagg caccaaactt
      121 attataataa ataagaacgc ccgacaatat aattccaaaa attaatagga cactggcagc
      181 aagcagtcca atgaaaattc tcttttttat ttttgtactt tctccgtttc tattgatagt
      241 cagcaaacaa atctccctct ttaaaatggt ggccaattaa atattctgtt gctttcatcc
      301 gtttactacc ggcttcctga agttctgtta ccagtatgtc acctgtaccc gtgcccacga
      361 gaaaaccttc gttttttacc aagcctctat acctgcccgg cgctggagca ttgccggatg
      421 ggaagggccg tgctttaaat atttttattt ccctgccctt cagtatggtg tacgcacagg
      481 gccagggata ggtgcctctt atgagattgc agatctcatt ggcactcttg ctccaatcaa
      541 tcttgccaat ctcctttttc agcattggag cataagttgc ttgtgtatca tcttgggcta
      601 ttcttattat attgccggat tttatatgct ttaaagtatc gataagcaac ttgccgccta
      661 aaatagataa ttcactgaaa agttcaaggg cagtccagtc gtcttttatt tctattgact
      721 cctgtaagaa aatgtctccc gtgtccaggc cttcgtccat ccacattgtg gtaactcctg
      781 tgaccttttc accgttaatt atagcccact gcaccggggc agcccctcgg tattttggca
      841 gcaaggaagc gtggacattg atacagccta ttttgggaat gtctaggacc tgtcgggaca
      901 gtatttgacc aaaagccacc acaacgatta aatcgggctc aagggaaagc aattttttta
      961 taaattcctc atcccttacc ttggcgggct ggtaaactgg aatattatgc tcaatagcca
     1021 gttttttaac cggcggcggg gtaattattc gctttctacc tacgggccta tccggttggg
     1081 tgacaactgc tacaatatct attctttctt ttagcaaaat atcaagggaa gcaaccgcaa
     1141 attccggagt gcccataaat acaactctca tcattatccc tcgctatcag gctctagaaa
     1201 ttttgtaact ttatcaataa agagtatccc atccaagtgg tctatttcat ggcaaaatgc
     1261 ccgggcaagc aaatcttccc cttcgatttc aaaagtttta ccccatctat cctgtgccct
     1321 tagcttcacc cgcttaggtc ttggtacttc acctaacaca cctggcacgc taagacatcc
     1381 ttcaggctgt gagtcttcac cttccgtttc aataatttca ggattcacaa gttcgatggg
     1441 gccatctcct acatctatta ccaccactct tttcaatatg ccaacttggg gggcggcaag
     1501 tccaacccca tcagcttcct tcatggtttc aagcatatca tcgatgagag tatgcaggcg
     1561 ctcgttaaaa tgctctactt ttttcgattt tttccgtaaa atatcgtcgc caatttctct
     1621 gatacttctt aatgccaaca gtttccctcc taaagcaaat ctagcggttc caggtcccat
     1681 gcaatatcca cgtgcctgtt tatacaagtc ttttgcatgt gattacaaat ttcgcttaag
     1741 acccgtactt gtttgctctt taataatata ttgtacctgt agttttcttt gatctttgat
     1801 ctagctgcgg ggaccggacc gtacattttt acttcgttat tatacctttc agatagtact
     1861 ttttttacat cttcagcttc ctttttaact acatcctcat tatgcccttt aaacctaata
     1921 atcagcatgt ggcaaaaagg aggataaagg gccttgtggc gcattttaag ctcctcttta
     1981 taaaaagcag aaatatccat actacaagct gcctttatgg cataagcttc gggagcatac
     2041 gtttgcacgt aaaccctacc gggagtatca cctctacctg cccttcccgc cacttgagtt
     2101 atcagctgaa aagtgcgctc accacaccta aaatccggca tgttaagagc agtatcggca
     2161 ctgacaatcc ctacgagggt aacattggga aaatcaaggc cttttgccac agtctgagta
     2221 cctattagaa tttgagctgc ccctctttta aagctaagca gcattttttc taaggaaccc
     2281 tttttagagg tagaatcaga atcaatccta agggtcttgc aagcgggaaa aaacttttta
     2341 acttcctgtt ccaccttttc tgtacctgct ccaaaataac gtatattttg ccctctgcac
     2401 acagggcaaa agtcgggtgc ctttacagtg tagccacaat aatgacattt tgcgttttta
     2461 tcgttgaagt gatatgtcaa tgatatatcg cagtacttac attttaatac gaatccgcaa
     2521 tcgcggcata ttacaaaagt agagtggcct ctgcggttta aaaagagaat tacctgtttg
     2581 ttggcatcca gggttgcctt gatgcttgat aaaagttttc gactaaagat atggcggttc
     2641 cctgatttta gttccgatat catatcgatg atttccatct ttggcaagac ccttccagag
     2701 actcttcttg taagtttgac aagggagtaa tctccctttg ctgccctgta aaatgattct
     2761 acagaaggtg tagcgcttcc catgatgagc atcgcatttt caagttttga gcgaaatctg
     2821 gccacgagtc ttgcatcata gtaaggatgt tcaccgtttt tatatgatgc ctcatgctct
     2881 tcatctataa tgattagacc tagattctgt actggggcaa aaattgcaga ccttgcccct
     2941 ataactacgg ttgcacttcc ggaatgtatt ctttgccact cgtcaaatcg ctctccagga
     3001 gaaagtctac tgtgaatgac agcagttttc ccgggaaatc tctggcgaaa tattgagagc
     3061 atttgtggtg tcaaagagat ttcaggaact aataccagta cactctcccc ttgactcagc
     3121 accctttgta taacccttat gtatacttca gttttaccgc tcccggtgac gccgtatata
     3181 agtgtggttt tttttgaacg ggagaaaata tcactgattg tttctaccgc atcatgctgt
     3241 tcatctgtta aactgatgcg attgtgatat ttttcgctgc tgaaagccat aggcattctt
     3301 ctaacagctc tgtactcttt tttaacaatt ccctttttat ctagagacgt caaagcagat
     3361 ctaaccgaag caaggggaat atctagcttt ttagaaaggt tttgtgcagt atcggcccca
     3421 ttttctatga gaaattcata tacatcttcg taattcttag caaaactaat ggatatctta
     3481 cgctttttca catttgacct ccaggatggt taagaagcta aggacattgg caaattgagc
     3541 gtcatctaca ata
//
LOCUS       DAONUO010000218         3553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18262_length_3553_cov_3.519726, whole genome shotgun sequence.
ACCESSION   DAONUO010000218 DAONUO010000000
VERSION     DAONUO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18262_length_3553_cov_3.519726"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PK768_07940"
     CDS             complement(<1..725)
                     /locus_tag="PK768_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HQA60941.1"
                     /translation="MNTKQRYTNKLISEKSPYLLQHAHNPVDWFPWGDEAFEKAKGEE
                     KPIFLSIGYSTCHWCHVMARESFEDDEVAELLNKHFVSIKVDREERPDIDMIYMNACQ
                     AMTGQGGWPLTVVMTPDKKPFFAGTYFPKENRWGRPGLMDILKQLIEVWEKDRDRIDA
                     VGDKVTQVLQSQGQGTPGKLDESILEEAFGEFSINFDAKYGGFGSAPKFPIPHNILFL
                     LRYWHKTRDNKALLMAEKTLDAMA"
     gene            886..1248
                     /locus_tag="PK768_07945"
     CDS             886..1248
                     /locus_tag="PK768_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQA60942.1"
                     /translation="MKTYKLTEMEGKLSDLIWANEPIPSGELVKLCKKRFDWKKSTTY
                     TMLKRLENKGIFQNRNGLVSSLIKKDDFYAKQCKQFVKENFEGSLPKFLAAFTRNTKL
                     SDDEISALLKLIHEHKEG"
     gene            1251..2891
                     /locus_tag="PK768_07950"
     CDS             1251..2891
                     /locus_tag="PK768_07950"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQA60943.1"
                     /translation="MDKLFLQVLKMSITSSYVILFVIVARIFLKKAPKFFSYALWFIV
                     LFRLIAPFSFESIFSLIPFNTQTVPTNVVYSQISREIRSTGQVTNNSLSGPVAHTNGK
                     PIQLWIELGEIVWLLGMLAILTYSFYTTVELYIKLQNAEHVSKNVYELTGLKTPFVFG
                     ITKPKIYLPAGLSNHEKSYIIKHEQTHIKRLDHLAKLLAFIVFSIHWFNPLVWLAFFL
                     MTEDMELSCDESVIRELGSEVKKDYSTSLLMMSTGRRIIGASPLTFGENNVRRRIINI
                     LNYKKPRFWVVVIATIAVLTVGFGLITNPRTVPSNSNAEKIQKAETWAEALKTRDGKS
                     RYAIMSENMKEQFVAEQKKRSGEDWNYNIGGSSPWVVDYDIDIHRDTATITYHFADSD
                     GVKYERTEIITFGRENNRLVIISAIEKIAEWDRVNYFAPNAKRAMEVYVEGLLESDYL
                     KLLSLAHEAPFDPAGQDIWDTIKIIDVKVIREDIRENKACYELQLDIEDGGNSAFEKG
                     ISTRWLWLVKGEHGWYVEGFMTGGAPDASWWNSESIKK"
     gene            complement(2944..3534)
                     /locus_tag="PK768_07955"
     CDS             complement(2944..3534)
                     /locus_tag="PK768_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQA60944.1"
                     /translation="MVTRGSKTYENLMKAFAGESQARNRYNISTAIAKKEGFYVVASV
                     FDYTADQERAHAKVFYQLLKEFAGDNISISGAYPVDLYDSTAQALRSAQHNELEEWEK
                     VYRSFGIVAKEEGFTEVAGIFERIANIEKVHADRFTRLADEIESGTIFKKDEEIPWIC
                     TVCGNIHVGKEPPEVCNVCQHPKGFYMPFSESFAES"
BASE COUNT         1086 a          695 c          675 g         1097 t
ORIGIN      
        1 gccatcgcat ccagggtttt ttctgccatt agtaaagctt tgttgtctct agttttatgc
       61 cagtacctta gcaaaaacaa aatattgtgc ggtatgggaa actttggtgc gctgccaaaa
      121 ccgccatatt ttgcatcgaa gtttattgaa aactcaccaa aagcctcttc taaaatcgac
      181 tcatctaatt tgccgggagt cccttggccc tgactttgca aaacttgtgt cactttatcc
      241 cctaccgcat ctatacggtc cctgtccttt tcccacactt caattaactg ttttaaaata
      301 tccattaaac cggggcggcc ccatctgttt tcttttggaa agtaagttcc tgcaaaaaag
      361 ggctttttat caggggtcat gacaactgtg agaggccatc ctccttgacc ggtcatagct
      421 tgacatgcat tcatatatat catatctatg tccggccgct cttccctatc tacttttatt
      481 gaaacaaagt gtttgtttaa aagctcagcc acttcatcat cttcaaaaga ttctcgagcc
      541 atcacatggc accagtgaca ggtcgagtat ccgatggaca gaaagatagg tttctcttca
      601 cccttcgctt tttcaaatgc ttcatctccc cacggaaacc aatctaccgg attgtgagca
      661 tgctgcaaaa gataagggga tttttcagaa atcaatttgt tcgtatatct ttgttttgta
      721 ttcaaattat cacatccttc tgtaacatta ttgttagtat atgaaggttt gaaatcagta
      781 tgcaaagtta aaataacatc tcttgacaaa caggtctaca agaagtagac tataaatata
      841 gctaggtcta caaaatgtaa accttttgaa aaggagatag atagaatgaa aacgtataaa
      901 cttaccgaga tggaagggaa actctcagac ctgatttggg ctaacgaacc aattccttcg
      961 ggagaattgg tgaagctatg taaaaagagg tttgactgga aaaaatcaac cacttatact
     1021 atgttaaaac gattggagaa caaaggcatt tttcaaaata gaaacggcct agtatcttca
     1081 ctcataaaaa aagatgattt ttacgctaag caatgcaagc agtttgtaaa agaaaacttt
     1141 gagggttcct tgccgaagtt tttagctgcc tttactagaa atacaaagct atctgatgat
     1201 gagatttcag cactgctgaa attaatacat gaacacaagg agggctagtt gtggataaat
     1261 tgtttttaca agtgctgaag atgagcatta catcttccta cgttattctg tttgttattg
     1321 tcgctaggat atttcttaaa aaagcaccta agtttttctc ctatgcactg tggttcattg
     1381 tactatttag gctaatagcc cctttttcat ttgaaagcat atttagcctc atacctttca
     1441 atactcaaac agtacctact aacgttgtat actcccaaat ttccagggaa attaggtcaa
     1501 caggtcaagt gacaaacaac tctttgtctg gacctgtcgc ccacactaat ggaaagccta
     1561 tacaactttg gattgagcta ggtgaaattg tatggctgct tggtatgtta gcgatactta
     1621 catatagttt ctacacaact gtcgagttat atatcaaatt gcaaaatgca gaacatgttt
     1681 ctaagaatgt atatgaatta actggattaa aaacaccttt cgtttttggt attactaaac
     1741 ctaagattta tcttccggca ggtctttcta atcatgagaa atcctatatt attaagcatg
     1801 agcaaactca tattaaaaga ctcgaccatc tggcaaagct cttagcattc atagtgttta
     1861 gtatccactg gtttaaccct ctagtttggc ttgccttttt tcttatgacc gaagacatgg
     1921 agctatcctg tgatgaaagt gttattagag aactgggaag tgaggttaaa aaggattatt
     1981 ccacttccct tttaatgatg tccacaggta gaagaattat cggtgctagt ccccttactt
     2041 ttggagagaa caatgttaga agacgaatca taaacatctt aaattacaaa aagccgaggt
     2101 tttgggttgt tgttatagca actattgcag ttttaactgt tgggtttggg cttattacaa
     2161 atcctagaac cgttccaagt aatagtaatg cagaaaaaat tcagaaggct gaaacctggg
     2221 cagaagcttt aaaaactcgg gatggaaaat cccgttacgc aatcatgtct gaaaacatga
     2281 aagagcagtt tgttgctgag caaaaaaagc gttccggtga agactggaat tataatattg
     2341 gaggttctag cccatgggtt gtcgactacg atattgatat acatagagac acggcaacta
     2401 tcacttacca tttcgctgac agtgatgggg taaaatatga gcgaactgag ataattactt
     2461 ttggccgaga aaacaaccgt ctagtcataa tatctgctat agaaaaaatt gctgaatggg
     2521 accgagtaaa ttattttgcg ccaaatgcta agagggcaat ggaagtttac gtcgaaggcc
     2581 ttctcgaaag tgattattta aaactacttt ctctcgccca tgaagcgccc tttgatccgg
     2641 ccggccagga tatttgggat acgataaaaa ttattgatgt aaaagtaatc agggaagata
     2701 ttcgggaaaa taaggcttgt tatgagttac aactagatat tgaagatggc ggaaattccg
     2761 cttttgaaaa aggcatctcc acacgttggt tatggcttgt aaagggagaa catggttggt
     2821 atgtggaagg ctttatgact ggcggagctc ctgatgccag ttggtggaat tccgaaagca
     2881 tcaaaaaata atactgatgt acacaagatt aaacgctaat catgaccact cgtaaacggg
     2941 tggtcatgat tctgcaaagg attcagaaaa gggcatataa aatccttttg gatgttgaca
     3001 aacattacaa acttccggag gttctttgcc tacatggata ttaccgcaaa ctgtgcatat
     3061 ccatggaatt tcttcatctt tcttgaaaat ggttccactt tctatttcat cagccagcct
     3121 tgtaaatcta tctgcatgga ccttttcaat atttgcgatg cgctcaaaaa tcccggcaac
     3181 ttctgtaaaa ccctcttctt ttgctactat accaaagctc ctatatactt tttcccattc
     3241 ttcaagctca ttatgctgtg cagacctaag agcctgagcg gtgctatcat ataaatcaac
     3301 gggataagct ccagatatgc ttatattatc tcctgcaaac tctttgagca attgataaaa
     3361 gacttttgca tgagctcttt cctggtctgc agtataatca aatactgacg ccacaacgta
     3421 aaagccctct ttttttgcaa tggctgtaga aatattgtat cggtttcttg cttgactttc
     3481 tcccgcaaat gccttcatta aattttcata tgtcttactt cctcttgtaa ccatacatat
     3541 cccccattat gag
//
LOCUS       DAONUO010000219         3539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18361_length_3539_cov_3.481056, whole genome shotgun sequence.
ACCESSION   DAONUO010000219 DAONUO010000000
VERSION     DAONUO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18361_length_3539_cov_3.481056"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK768_07960"
     CDS             complement(<1..344)
                     /locus_tag="PK768_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQA60945.1"
                     /translation="MLDYVEGKLVKREPNFAIIDVGGWGVKLSVSLFTYNKIKDFKDQ
                     NVRLYTHMVLRDDAIELYGFFDEVERQAFVLLNRVPGIGPKVAISILSLMDISHLKTS
                     VISEDVNALVTVP"
     gene            complement(355..846)
                     /gene="ruvC"
                     /locus_tag="PK768_07965"
     CDS             complement(355..846)
                     /gene="ruvC"
                     /locus_tag="PK768_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQA60946.1"
                     /translation="MLIMGIDPGIAISGYGLISCENSGCRILEYGVIKTSAGLETPQR
                     LRQIHEGYLSLIERYKPDAVAIEELFFNKNAKTIITVGQARGAALLAAALSNIEVFEY
                     TPLQVKQAVVGYGRADKHQVQQMIKIFFHLDELPKPDDAADALAVAVCHMNSYKLGKI
                     LYS"
     gene            complement(908..1465)
                     /locus_tag="PK768_07970"
     CDS             complement(908..1465)
                     /locus_tag="PK768_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BofC C-terminal domain-containing protein"
                     /protein_id="HQA60947.1"
                     /translation="MPGKVFRYIVIITLITAMSFGYGYLKTFLSIKEPNKNTNFNNIE
                     SNVSQISDVKLNPGAKLIYTSHYVKCDDKIIQEEIIGDDLSGCTRDELKELKKEWEIV
                     SFAPDEVSLIRVINDVCDNHYYIGIQDGYVALFQGIPGIKAILVEKTDIIADTLREDD
                     RLILEKGLTIKDQEEFLKIREGLAK"
     gene            complement(1538..2269)
                     /locus_tag="PK768_07975"
     CDS             complement(1538..2269)
                     /locus_tag="PK768_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQA60948.1"
                     /translation="MAGHSKWSNIKHKKAKMDAQKGKIYSRFSKLIIIAVREGGPDPN
                     ANSRLKDIIEKAKQANMPNDNITRAIKRGSGELGGGDIEEIMYEGYGPGGVAILVDAT
                     TDNRNRTAGEMRHLFDKYGGNLGESGCVAWMFERKGLILIEKKDGIDVDEIMMLAIDS
                     GAEDVEETEDSIVITTAVDSFEMVKNTLTESNIEIFSADLSFIPNSTISVSEEDNERL
                     EKLLESLEDHDDVQNVYTNYEPEEN"
     gene            complement(2342..3052)
                     /gene="nadE"
                     /locus_tag="PK768_07980"
     CDS             complement(2342..3052)
                     /gene="nadE"
                     /locus_tag="PK768_07980"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQA60949.1"
                     /translation="MNKLCGSLVSWLQEYVKQAGADGAVFGMSGGIDSAVVSVLCKKA
                     FNENALGLIMPCFSDKDDELDARFVAEKFGIKYQTVVLDKVYDEFLKAMMPTDNIMAK
                     ANIKPRLRMTVLYYYAAINNYLVVGSENKTELTVGYFTKYGDGGADLWPIGNLVKSQV
                     RELAAFLGIPQKIINKAPTAGLWSGQTDEQEMGITYDELDKYILTGSLNSSEKEAKIK
                     AMHNCSEHKRRLALIPDV"
     gene            complement(3101..>3539)
                     /locus_tag="PK768_07985"
     CDS             complement(3101..>3539)
                     /locus_tag="PK768_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQA60950.1"
                     /translation="ELQRFSDDGEPSGTAGKPVLEVIKSTDVKNVLIVVTRYFGGIHL
                     GASGLIRAYSESASRGLKNSKIVKKIKCDVFEITTDYNMLGKLQWELNQKDLIIKDIK
                     YAEDVKMEINVPKNYPIDIWQFILDLTSGNADVKLVGSEYIVK"
BASE COUNT          975 a          840 c          527 g         1197 t
ORIGIN      
        1 ggcactgtga ccagggcatt tacatcttcc gagataacgc tggtttttaa gtgcgatata
       61 tccattaaag aaagaattga aatagccact tttggaccaa tccctggcac tctatttaaa
      121 agcacaaacg cttgtctttc aacctcatcg aaaaagccat aaagctcaat cgcatcgtcc
      181 ctcaaaacca tatgggtgta aagtctaaca ttttgatctt taaagtcctt tatcttgttg
      241 taagtgaaca gtgaaacact aagtttgacc ccccaacctc ccacatcaat tatagcaaaa
      301 tttggttccc ttttaacaag tttcccttct acataatcta acatgcctta tacctcaact
      361 gtacaatatt ttcccaagct tatatgagtt catgtggcat acagccactg ccaaagcatc
      421 tgcagcatca tctggcttcg gcaattcatc caaatgaaag aaaatcttaa tcatctgctg
      481 aacctgatgt ttatctgcac gtccgtagcc cacaaccgct tgcttaacct gtaagggcgt
      541 atattcaaac acttcaatat tagataaagc tgcagctaaa agcgcagctc cccttgcttg
      601 gccaacagta ataatggtct ttgcattttt attaaaaaaa agctcttcta tggcaactgc
      661 atcaggtttg tatctttcta ttaagcttaa ataaccttca tgtatttgtc ttaacctttg
      721 aggggtttca agacctgcag atgtttttat aactccgtac tctaaaattc tacagccgct
      781 attttcacaa gaaataaggc catatccgct gatggcaata cccgggtcaa tccccataat
      841 taacataaaa tcacccttag tgtaattcta catccaaggg tgattttcct tcttgtatga
      901 aaaatagcta ttttgcaagg ccctctctaa ttttcaaaaa ctcctcttgg tcctttattg
      961 taagaccctt ttctagaatc aacctatcgt cttccctcaa agtatctgct atgatatccg
     1021 tcttttcaac taatattgcc tttatacccg gaatgccttg aaaaagagcc acatagccat
     1081 cctgaatgcc gatataatag tgattatcgc atacatcatt tattacccta atcaagctga
     1141 cttcatccgg tgcaaagctt actatctccc actctttttt caattctttc aattcatccc
     1201 ttgtacagcc actcaagtca tcgccaataa tctcttcttg tattatttta tcatcgcatt
     1261 tcacgtaatg gctagtataa attaatttcg caccggggtt cagtttcaca tcagaaattt
     1321 gagaaacatt gctttcgata ttattgaaat tggtattttt attaggctct tttattgata
     1381 aaaaggtctt taaatacccg tacccaaaac tcattgcggt aataagggtt atgattacta
     1441 tatacctaaa aacttttccg ggcataccat attcctcctt ttactcttcg gatgaatagt
     1501 atgcccattt cttgcaataa tatacagttt tttctattta attctcctcg ggctcataat
     1561 ttgtatatac attttgtaca tcatcatggt cttcaagaga ttctaacaat ttttctagtc
     1621 tttcgttatc ttcttcggat acggagatgg tcgagtttgg tataaaggac aaatctgccg
     1681 aaaaaatctc gatatttgat tccgttaatg tgtttttgac catttcaaag ctgtcaacag
     1741 ctgttgtaat tacaatagaa tcttcagttt cctccacatc ttcagcgcca gaatcaatgg
     1801 ccagcatcat gatttcatca acatctatac cgtctttctt ctcaattaaa attaagcctt
     1861 ttctttcaaa catccaggca acgcaaccgc tttcacctaa gttcccaccg tatttgtcaa
     1921 agagatgcct catttccccc gccgtccggt ttctattgtc tgttgtggca tcaactagga
     1981 tagcaacacc gccggggcca taaccttcat acataatttc ttcgatatct ccgccgccta
     2041 actctccgct tccccttttt atagctctcg ttatattgtc atttggcata tttgcctgct
     2101 tggccttttc aattatatcc tttaaccgag agtttgcgtt tgggtcggga ccaccttctc
     2161 gaacagctat tataataagc ttgctaaatc tagaatatat ttttcccttt tgagcatcca
     2221 tttttgcttt tttatgcttt atgtttgacc acttagaatg tcctgccatg taatatcccc
     2281 ctttaaccat ttccttaatt atttaatcac aaaagtcatt cctagctttt atgtcttcag
     2341 actaaacgtc cggaataagg gctaaacgcc gcttatgttc gctgcaattg tgcatggctt
     2401 ttattttggc ctctttttct gaagagttca agctcccagt taaaatgtat ttgtccagtt
     2461 catcataagt tatccccatt tcctgctcat ctgtttggcc gctccaaagc cctgccgttg
     2521 gagctttatt gataattttt tgcggaatcc ccagaaaagc cgctagttcc ctaacctggc
     2581 tttttaccag atttccgatt ggccataaat cggctcctcc gtctccatat tttgtaaaat
     2641 agcctacggt gagctcagtt ttattttccg aacctaccac aagataatta ttaatggctg
     2701 catagtaata caagactgtc atcctgagtc gtggcttgat atttgcctta gccattatat
     2761 tgtctgtcgg catcatcgct tttaaaaact catcgtaaac tttatcaagg actacagttt
     2821 gatactttat gccaaatttt tcagccacaa aacgagcatc caactcatcg tctttatcgc
     2881 taaagcaagg cataatgagc ccaagagcat tttcgttaaa ggcctttttg cataacacgc
     2941 taactaccgc cgaatcaatt cctccgctca tgccaaatac ggctccatct gcacccgctt
     3001 gctttacata ttcttgcagc caagatacta atgaaccaca aagtttattc atgttcaaga
     3061 taaagcaccc cttgaaatga tttatacgta ctatttatat ttattttaca atgtattcac
     3121 tgcccaccaa cttaacatct gcattccccg aagtcaaatc caagataaac tgccatatgt
     3181 caatgggata atttttcgga acattaatct ccatttttac atcttcagca tatttgatat
     3241 ctttgattat aaggtccttt tgattcaatt cccattgtag ctttcccaac atattgtaat
     3301 cggtagttat ttcaaataca tcgcatttta tcttttttac gatctttgaa ttcttaaggc
     3361 ctcgggaagc gctttcgctg taagctctta tcaggccact tgcacccaaa tgaataccgc
     3421 caaaataacg tgtgaccact atcaagacat tctttacgtc cgtagatttg ataacctcca
     3481 gcacaggttt gccggcagtg ccggaaggtt ctccatcatc gctaaatctt tgtaattca
//
LOCUS       DAONUO010000220         3528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18408_length_3528_cov_3.242730, whole genome shotgun sequence.
ACCESSION   DAONUO010000220 DAONUO010000000
VERSION     DAONUO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18408_length_3528_cov_3.242730"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1141
                     /gene="uxuA"
                     /locus_tag="PK768_07990"
     CDS             65..1141
                     /gene="uxuA"
                     /locus_tag="PK768_07990"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF003027.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HQA60951.1"
                     /translation="MKMVFRWFGKGYDNIPLEYIRQIPGVTGVVATLMDIPAGEVWPI
                     ERVKALKEEINKHDLEIEVIESVNIHEDIKLGESSRDKFIENYIETMKNLSQIGVKVI
                     CYNFMPVLDWARSELARELPDGSNTMAYKHEHILNIDPQNLVSEVDKQSGGFELPGWE
                     PERLKEMSITIEKYKDMTEDQYWKNIKYFLDSVIPYAEKYDIKLAIHPDDPPWPIFNL
                     PRVITSRDNIRRFLDLNNSPYNGITLCTGSLGANKNNDIPAIIREFAAEGRVHFAHIR
                     NLKFESEKDFYESSHLSQEGSLDMFEIVKAFYDTGFTGYIRPDHGRMIWGETGRAGYG
                     LYDRALGATYILGLWEAIDKMTKK"
     gene            1197..2132
                     /locus_tag="PK768_07995"
     CDS             1197..2132
                     /locus_tag="PK768_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQA60952.1"
                     /translation="MKKKILITPKSFFRARDKAEEIFSKYPLEVVENNTGKTLTKGQM
                     IELCEDIDGIIVGIDPMDEEVLRNASKLKAISKYGVGLDNIDLKVAKELGIKICIAEG
                     TNTRSVAELTIGLFFTISRSIAKATYDVKDGRWDRTLGVELLGKTVGIIGFGAIGREV
                     AKMSAGLSMNIMAYDPYFNDVRLAQSMKVEMADIMEILEKADFVTLHLPLNEETNKII
                     NSEALSKMKQTAFLINTSRGELVDEDALYDALVKGAIAGAAQDVFSQEPPNKDNKLLS
                     LDNFILTPHIGAYTKESTERMVVSSAKNLVEMLFD"
     gene            2322..3038
                     /locus_tag="PK768_08000"
     CDS             2322..3038
                     /locus_tag="PK768_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQA60953.1"
                     /translation="MLEKLKRNKREYTREYVMRVLKHNIINLTLKPGQLVSENEIAEV
                     LGVSRTPVREAFIELSRASLVEIYPQKGTCISPIDMDVVEETRFMRCAVEKAIVKLCC
                     DVLTDSDFAYLQENLQLQESCISSKKYLYLLSLDDKFHEYMFKACKKKKIYGLIKSAM
                     AQFDRVRILNFVEMDMEISVNEHKKIFHAMKNRDKELAERLMEEHLTRVIFDMSYLKE
                     KHPEYFKDDSANTSSIAQIF"
     gene            3328..>3528
                     /locus_tag="PK768_08005"
     CDS             3328..>3528
                     /locus_tag="PK768_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HQA60954.1"
                     /translation="MDDELLMSKVCKMFYYQQLSKVEIAEKLRISRFKVARLLKKAQE
                     VGIVKIQIVNPRSDMFPLEERLE"
BASE COUNT         1217 a          614 c          778 g          919 t
ORIGIN      
        1 tgaaaatcta agaataggtg agatatttaa acataaataa agattataga gggagttgat
       61 gtcaatgaaa atggtattta gatggtttgg gaaaggatac gacaacattc cccttgaata
      121 tatccggcag atacccggtg tgacgggtgt cgtggcaacc cttatggata tacctgctgg
      181 cgaagtctgg cctatagaga gggtaaaggc cttaaaagag gaaattaaca agcacgacct
      241 tgaaattgaa gtgatcgaaa gcgttaacat ccatgaagac attaagcttg gcgaaagcag
      301 cagggataaa tttatagaaa actatattga aacaatgaag aatctatcac aaatcggtgt
      361 caaggtaatt tgctacaact ttatgcctgt ccttgactgg gcgagaagtg aactcgcaag
      421 agagcttcct gatggttcca acaccatggc atacaagcac gaacatattt taaacataga
      481 tccgcaaaat ctcgtaagtg aagtagataa gcaatctggt ggttttgaac tgcccggatg
      541 ggagccggag agattaaaag agatgtcgat tacaattgaa aagtataagg atatgaccga
      601 agatcaatat tggaaaaaca taaagtattt ccttgacagc gttattccct acgcagaaaa
      661 gtacgatatt aaactggcaa tccaccccga tgatccaccc tggcccatct tcaatctacc
      721 gagagtgatt accagcaggg acaacatacg cagattcctt gacctgaaca acagcccata
      781 taacggcata accctttgca caggttccct gggggcaaac aagaataatg atattcctgc
      841 catcataaga gagtttgcag ctgaaggcag agtccatttt gcccatatca gaaacctgaa
      901 gtttgaatct gaaaaggact tttacgaaag ctcccacctt tcccaagaag gttcccttga
      961 tatgtttgaa atagtgaagg ccttttacga tactggcttt acaggatata ttaggcctga
     1021 ccacggcaga atgatttggg gagaaactgg ccgagccggt tacggccttt atgacagggc
     1081 cttaggtgcc acctatattc tcggtctgtg ggaagcaatt gataagatga ccaagaagta
     1141 atataagata ataatgatgc tataacttaa atgcggataa ttcgttggag gtataaatga
     1201 aaaagaagat attgatcact cccaaatcat tttttcgcgc tagagacaaa gctgaagaga
     1261 ttttcagtaa gtacccgctg gaagtagttg aaaacaacac gggaaagacc ctgacaaagg
     1321 ggcaaatgat tgaactgtgc gaggatatag acggaatcat cgtcggcata gaccctatgg
     1381 atgaagaagt gctaagaaac gcaagcaagc tcaaggcaat atcgaagtac ggtgtcggcc
     1441 ttgacaatat agatttaaag gttgcaaaag agctgggcat aaaaatatgt atagcggaag
     1501 gtaccaatac gcgttcggtg gcggaactga ccataggcct gttctttacc atatcccgaa
     1561 gcatagccaa ggccacttat gacgtgaaag atggcagatg ggatagaact ttaggcgttg
     1621 aacttctcgg aaaaacggta gggataattg ggtttggagc aattggccgc gaagttgcga
     1681 aaatgtcagc cggcttgagc atgaacataa tggcctatga cccttatttt aatgatgtaa
     1741 ggcttgcaca atccatgaag gtagagatgg cagacataat ggaaatactg gaaaaagctg
     1801 attttgtaac actgcacttg cccttaaatg aagaaacaaa taaaataatt aacagcgaag
     1861 ctttgagcaa aatgaaacaa actgcatttc taattaatac ttcaagaggc gaactggtag
     1921 atgaagacgc tctgtatgat gcgctggtaa aaggagctat tgcaggagca gcccaagatg
     1981 tgttttccca ggagcctcca aacaaagaca acaaattatt gtcattggat aactttattc
     2041 tcacacctca cataggggct tacaccaagg aatcaacgga gcgaatggtg gtaagctctg
     2101 caaaaaatct agtggaaatg ttgtttgatt aaatggtaaa attcttgggc tgcctttcac
     2161 tctattttca aaaagcttgt gggcaaggca acaaactgct tgtatataat atcggcagta
     2221 gtagctagta tttttcattt ccacaaagaa ttggctttat attgatatgc tatcataatg
     2281 taatgggaat attatggaaa ctggagagat aggggagaac aatgcttgaa aagctgaaga
     2341 gaaataaaag agaatatacc agagaatatg tcatgagagt cttgaaacac aacatcataa
     2401 atttaacctt aaaaccggga caattggtta gcgaaaatga aattgccgaa gttttaggag
     2461 taagccgcac acctgtaagg gaagctttta tagagctgtc cagagcatcc cttgttgaga
     2521 tatatcctca gaagggcaca tgtatttcgc cgatagacat ggacgtggtt gaggaaacac
     2581 gctttatgcg ctgtgcggtg gaaaaagcaa ttgtaaaatt gtgttgcgat gtcctgacag
     2641 acagtgattt tgcttacttg caggaaaatc ttcagctaca ggagtcctgt atttcctcca
     2701 aaaaatatct ttacctactt tcactagatg ataaatttca cgagtatatg tttaaagctt
     2761 gtaaaaagaa gaagatttac ggacttataa aaagtgccat ggcccaattt gaccgtgttc
     2821 gaatattgaa ttttgttgaa atggacatgg agatatccgt gaacgaacac aagaaaatat
     2881 tccatgctat gaaaaataga gataaggaac tggcagaaag gctaatggaa gaacacctta
     2941 ccagggttat tttcgacatg tcctacttaa aggagaagca tcccgaatat ttcaaagatg
     3001 attcagcaaa tacatctagc attgcgcaga ttttttaaca accggtagcc taaaaagcaa
     3061 ataactttta acatatataa gacatcgtaa aacaactttt gcggtgtctt tttgtttgcc
     3121 caaaaaagct ttatcccaag agcaatcata agctaacttc gattttatca tgtgtttgat
     3181 aattttttgt tgcgccgtca attatttaac agaaaaacta cacataattt taatatattt
     3241 gcttgacatt tattcaatca gaggctaaaa tataattaaa gatatcaaat gatatataaa
     3301 tgctcatatg agcaacagga ggaaatcatg gatgatgaac ttttgatgtc aaaagtgtgc
     3361 aagatgttct actaccaaca gctttccaaa gtcgaaattg cagaaaaatt gcgcatatcc
     3421 aggtttaaag tggcaaggct attaaaaaag gcgcaagaag ttgggatagt gaaaattcaa
     3481 atagttaatc ctaggtcaga tatgtttcca ttggaagaac ggcttgaa
//
LOCUS       DAONUO010000221         3519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18474_length_3519_cov_3.730658, whole genome shotgun sequence.
ACCESSION   DAONUO010000221 DAONUO010000000
VERSION     DAONUO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18474_length_3519_cov_3.730658"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1276
                     /locus_tag="PK768_08010"
     CDS             83..1276
                     /locus_tag="PK768_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQA60955.1"
                     /translation="MTKTKEGGSLIIKLLASIIIGIVIGLIAPENLMIVLETIKHFLG
                     QIIFFTIPLVILGFIAPAIAELKANASRFMGITILLAYLSSVGAAAFAVIVGYIIIPH
                     LSIASNVGDLREIPAPIIELNVPQLFGVMSALVLALMLGLAVANTKAETMKKLLSEFQ
                     KIVLYIVQSIIIPILPIFIATTFAGLAYEGSITRQLPVFIQIIILVIMGHYIWLAVLY
                     ALGGAVSGINPLNVLKHYGPAYMTAVGTMSSAATLPVALRCIHKNEDIPRHIQDFVIP
                     LCSTVHLCGSVLTETFFVMAVSQILYGAMPPLNQMITFILLLGLFGIAAPGVPGGTVM
                     ASLGLITGVLGFDQSGVALMLSIFALQDSFGTACNVTGDGAIALMVTGIAKRYMKGDE
                     SAVQG"
     gene            complement(1448..2185)
                     /locus_tag="PK768_08015"
     CDS             complement(1448..2185)
                     /locus_tag="PK768_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HQA60956.1"
                     /translation="MDRNEARKKIWEELRKVAKPDSRFHWDFAEFIADYEGSEKGAEL
                     IRDLEVYKKAKVIFITPDNNMEKLREFAYKDKKTLLTTTYGINRGFQVIYPGDVPEGK
                     EELATTLDGMEKFMKSISLKEIKEKLGKVDLLVTGASAITPSGIRFGKGHGYFDLEWA
                     MLWEIQAVDPETPVIAVGHDCQVVDIDIETTPYDTIVDYIVTPTRVINVNSDIPKPSQ
                     GIIWDKLEKGMIENIPPLQELYDLKHK"
     gene            complement(2207..2329)
                     /locus_tag="PK768_08020"
     CDS             complement(2207..2329)
                     /locus_tag="PK768_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60957.1"
                     /translation="MIYSATFFSILMWVSLAGLVVDFAFQLWYNIVDFKNKTLW"
     gene            complement(2341..>3519)
                     /locus_tag="PK768_08025"
     CDS             complement(2341..>3519)
                     /locus_tag="PK768_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60958.1"
                     /translation="AISLSYIVFLPVSGRIKELIPEGNTIGEYARVRYGKIMYIVVLF
                     VSIVYMFCLLAANLIGAGIAFNYIGHVPLYIGVLVIGVPVIIYTSYGGLGAAIFTNGL
                     QAILITPMLFITALLAFRNSGGTVAIYNGIQEHAPDFLKVFHPTGLEFALMIIIAVSA
                     AELLNQALWQRVYSAKDIRVVKKGLVSASLMTFPMTIVAASFGLMAVAYNIELPHPSI
                     ATALMADNLLPPWASMIFMMIIVLAATSTASDALAGFSSIASLDIIKSFMPNLAPEKA
                     VLVGRISTVVFGVGAMLVAFKAPSILFLLLTADLVAAAAVIPVVAGLFSPNISGNAAA
                     LGTILGILIGLPLFLNNQHLLSFVTAIIVSSAVIFVYAKISPARFDFNKLKEHIRAVD
                     "
BASE COUNT         1068 a          778 c          706 g          967 t
ORIGIN      
        1 actaaataag taggaattac tcgcgtgagt acaattatat ttttattaat taaggtaaat
       61 taattaatag aggtgtggat ttatgacaaa aacaaaagag ggcggcagtt taatcatcaa
      121 gctgctggca agtatcatta tcggtatagt aataggcctt atagctcccg aaaacctcat
      181 gatagtcctt gagaccataa aacacttttt aggtcagata atcttcttca ccattcccct
      241 ggtcatcctg gggttcatcg cccctgccat agcggaactt aaagccaacg ccagtcgatt
      301 catgggcatt accattttgt tagcatacct atcatcagtc ggagctgctg catttgccgt
      361 gatagtcggc tatattatca taccccattt atccattgct tcaaatgtag gagacttaag
      421 ggaaatccct gcacccataa tcgaactaaa tgtacctcaa ttatttggcg tcatgagcgc
      481 cctggtcctt gctttaatgt tgggtcttgc agtagccaac accaaagctg aaaccatgaa
      541 aaaactcttg agcgaattcc agaaaatcgt cttatacata gtgcagagca taataatccc
      601 gatactgcca atatttatag ctaccacctt tgcaggcctt gcctacgaag gcagcataac
      661 aagacaactg cctgttttca tccaaataat aatactggtg ataatgggcc attacatatg
      721 gcttgccgtc ctatatgccc taggtggtgc cgtatccggc ataaatcccc taaatgtcct
      781 caagcattat ggacctgcat acatgacggc agtaggcacc atgtcaagcg ctgccacact
      841 acccgtagcc ttaagatgca tccacaaaaa tgaagacata cccaggcaca tacaggactt
      901 tgtcatacca ttgtgcagca ccgttcacct ttgcggctcg gtgctgacag aaaccttctt
      961 cgtaatggca gtatcccaaa tactgtacgg agcaatgcca cctcttaacc aaatgataac
     1021 atttatactc ctgctgggtt tatttggtat tgcggcaccg ggagtaccgg gtggaacggt
     1081 tatggcatct ctaggtctta tcacaggagt gctgggcttt gaccagtcag gtgtagcctt
     1141 gatgctttca atctttgcac tgcaggacag cttcggcaca gcttgcaatg tcacgggaga
     1201 tggagccata gccttgatgg tgacaggaat cgcaaaaagg tacatgaagg gtgacgaatc
     1261 agcagttcaa ggttaatctg atagaaatca agcacaatgc tatggaactt acaagagtat
     1321 gagagtaagc agctaaaaaa cacaataggg tcgtcagtat tgatataagt caacgaaaaa
     1381 aagtatccaa gaaataaaag tttcttggat acttttttgt gctatgtggt aatttttctc
     1441 ttactgtcta tttgtgtttt aaatcatata actcctgcag tggaggaata ttttcaatca
     1501 tgcccttttc cagcttgtcc caaattattc cctgtgatgg ttttggaata tcagagttta
     1561 cgtttattac cctcgtaggt gtgacaatat aatctacaat agtatcatat ggagtagttt
     1621 ctatatctat gtcaacaacc tgacagtcat ggccaacagc gattaccggc gtttcgggat
     1681 ctaccgcctg gatttcccac agcattgccc actccaaatc aaaataccca tggcccttgc
     1741 caaagcgaat gccggaaggc gttatggcgg aagctcctgt caccagcaaa tcaaccttcc
     1801 caagcttttc tttaatttct tttaatgaaa ttgacttcat gaatttttcc ataccatcta
     1861 aagtagttgc cagctcttcc ttgccttcgg ggacatcacc aggatatatt acttgaaagc
     1921 cccggtttat gccataagta gtggtaagca gtgttttttt gtccttatat gcaaactccc
     1981 taagtttttc catattgtta tcaggggtta taaatattac cttagctttt ttataaactt
     2041 ccaagtctcg aataagctct gccccttttt cactgccttc ataatcagct ataaattcag
     2101 cgaaatccca gtgaaatctt gaatcaggct ttgccacctt ccttaattct tcccaaattt
     2161 tctttctggc ttcgttgcgg tccattaaaa acccactcct ttgtctctac cataaggtct
     2221 tgtttttaaa atcaactatg ttgtaccata gctgaaaagc aaagtccacc actaaaccgg
     2281 ccaaggaaac ccacatgagt atactgaaaa aagttgctga atatatcata ttaacctccc
     2341 ctagtcaaca gctcttatat gttctttgag tttattaaag tcaaatctgg caggcgaaat
     2401 cttagcataa acaaatataa cagcagaaga cacgatgatc gcagttacaa aacttaataa
     2461 gtgctggttg tttaagaaca atggaagacc aatcaaaata cccagtattg ttcccaatgc
     2521 tgccgcattg ccgctgatat ttgggctaaa gagtccggcg acaacaggaa taactgctgc
     2581 tgcagcaact aaatcagctg tcaagagtaa aaacaagata gacggggctt taaaggcaac
     2641 cagcattgca cctactccaa agacgacggt agaaattctg ccaacaagaa cagccttttc
     2701 tggagctaaa ttgggcataa agcttttaat gatatcaagg gaagctattg atgaaaaacc
     2761 agccagagcg tcagacgctg tagaagtggc agcaaggacg attatcatca tgaaaatcat
     2821 cgatgcccat ggtggcaaaa ggttatcagc cataagggct gttgcgatgg aaggatgggg
     2881 caattctata ttgtaagcta ctgccattaa cccaaaagat gcagccacaa ttgtcatggg
     2941 aaaagtcata agagatgcac taacaagccc cttttttaca accctgatgt ccttggcaga
     3001 atacacccgc tgccaaaggg cttggtttaa tagctctgct gcagatacag ctatgatgat
     3061 catcagagca aattctaatc ccgtaggatg aaagactttc aggaaatctg gagcatgttc
     3121 ttgtatgccg ttgtatatag ctacagtgcc gccggaattt ctaaaggcta aaagtgcagt
     3181 tatgaagagc ataggagtta tgagaatcgc ctggaggccg tttgtaaaga tggcggctcc
     3241 aagtcccccg tagctcgtat aaatgatgac aggcacaccg ataactagaa cacctatgta
     3301 aagtggaaca tgacctatgt aattaaaagc aatgcctgca ccaatgagat ttgcagcaag
     3361 caaacaaaac atgtagacaa tactgacgaa aagcacaaca atgtacatta tttttccgta
     3421 tctaactctg gcgtattctc cgatggtgtt gccttccggg atcagttcct tgatacggcc
     3481 agaaaccggc aaaaagacaa tataggataa agagatagc
//
LOCUS       DAONUO010000222         3500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18604_length_3500_cov_3.594775, whole genome shotgun sequence.
ACCESSION   DAONUO010000222 DAONUO010000000
VERSION     DAONUO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18604_length_3500_cov_3.594775"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PK768_08030"
     CDS             complement(<1..513)
                     /locus_tag="PK768_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA60959.1"
                     /translation="MTKFQKAFKSLILPIAIVILWHFTSKAGLLNTYIVPPPREILQT
                     AGELIQKGTLAKHIMASLQRVFIGFLYAFLLAFPTAVLIGIVRPLEDYLDLALEFIRH
                     IPPIACIPMLILWLGIGEASKIAVIILAAFFPIFLNTLNGVMNCDPKLLEVGDVFGFN
                     VREKFFKIILP"
     gene            complement(488..1738)
                     /gene="hydF"
                     /locus_tag="PK768_08035"
     CDS             complement(488..1738)
                     /gene="hydF"
                     /locus_tag="PK768_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HQA60960.1"
                     /translation="MNETPRANRLHIAVFGRRNAGKSSLINAITGQEVALVSKHPGTT
                     TDPVYKAMELLPLGPVVFIDTAGLDDTGDLGRLRVKKTLAVMDKTDLALMVFSPENSS
                     FDLEKEWYEKLRERKIPIIGVVNKVDIKDTGIDKYSKDFDFPFVKVSAKEGTNIGKLK
                     EMISQYAPSNFELPALIGDILKPKSQVILVTPQDIQAPKNRLILPQVQTIRDLLDHNC
                     MALVAKDTELEDLLQSLKWLPDLVITDSQIFHRVNEIVPENVPLTSFSIIMSRYKGEL
                     GTFIKGAHAIDELKPGDRVLIAEACTHHPLENDIGRQKLPALLEKKAGGKLEIDVKAG
                     ADFPEDLTPYRLILHCGSCMLNRKQMLSRIIRAIQQDVPITNYGMAFAHAAGILKRAT
                     SMFGQASGSPLTTGGRHDKVSEGL"
     gene            complement(1743..2792)
                     /gene="hydE"
                     /locus_tag="PK768_08040"
     CDS             complement(1743..2792)
                     /gene="hydE"
                     /locus_tag="PK768_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HQA60961.1"
                     /translation="MEIYQLVQKICGGYHPGVDEVEKLLCAEGQDLDYIFKAADEINR
                     FYNGNTVHIRGIIEFSNYCRASCAYCGLNAGNKELKRYRMEPDEIIEVAKKAYDAGYK
                     TIVLQSGEDLWYSKEKISYIIREIKAIGGLAITLSVGERAYEDYRQWKEDGADRFLLK
                     HETINPRLYKSLHPHSTLANRILCLKRLKTLGYQTGSGFMIGLPGQTTRDIAKDILFL
                     NRLEVDMAGIGPFIPHQGTALRDAPAGSNEMTLKALAVTRLIVKTAHLPATTALRTLG
                     GQGAESAFHVGANVIMLKLEPYKYRRLYEIYPKDFGEEQSIREERREMEEYLKSMGKE
                     VARDKGDSLRLLERL"
     gene            complement(3139..3444)
                     /locus_tag="PK768_08045"
     CDS             complement(3139..3444)
                     /locus_tag="PK768_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA60962.1"
                     /translation="MRYTDVKDDAYYMGYIEWASENGIVKGIGDKKFPPDQAITREEL
                     AVIMSNFAKAMNLKLNHKYEENTFADSTEISAYAREAVKEMQMAGILSDKGIQMMKY"
BASE COUNT          797 a          894 c          756 g         1053 t
ORIGIN      
        1 tggcagaata attttgaaaa acttttcccg cacgttaaag ccgaagacgt ctcctacttc
       61 caagagctta gggtcacagt tcatgacgcc gtttaaggtg tttaaaaata tgggaaagaa
      121 ggccgccagg ataatgacgg ctatcttgga agcctctcct attcccagcc acaagatcag
      181 catgggaata caggctatag ggggaatatg cctgatgaat tctaaagcca aatctaggta
      241 gtcctccagg ggcctcacta tgccaatcag tacggcagtg ggaaatgcca gcagaaaggc
      301 atacaaaaag ccgataaata cccgctgcag gctggccatt atatgctttg ccaaggtccc
      361 cttttgtatt agctctcctg ccgtttgaag gatttcccgg ggcgggggaa cgatgtatgt
      421 atttaaaaga cctgctttgg aagtaaagtg ccaaagtatt acaattgcta tcggcaggat
      481 taggctttta aaggccttct gaaactttgt catgtctgcc tcctgtcgtc aaagggctgc
      541 cactcgcctg cccaaacatg gacgtggccc gctttaggat tccagccgcg tgggcaaagg
      601 ccattccgta attggtgatc ggcacatctt gctgtatcgc ccttatgatc ctggatagca
      661 tctgctttct gttgagcatg caggagccgc agtggagtat gagcctgtaa ggcgtcaggt
      721 cttccggaaa atccgctcct gccttaacat ctatttctag cttgcccccg gctttctttt
      781 ccaaaagggc cggcagtttt tgcctgccta tgtcgttttc cagggggtgg tgggtgcagg
      841 cttcagcaat caacaccctg tctcccggtt tcaattcatc aatggcatgg gcgcctttta
      901 tgaaagtgcc gagctcgccc ttgtatcttg acatgattat ggaaaaggat gtcaggggca
      961 cattttccgg gacaatctca tttacccggt ggaaaatctg tgagtcagtt atcaccaagt
     1021 cgggcagcca ttttaagctt tggagcaagt cctccaattc cgtgtccttt gcaacaaggg
     1081 ccatgcagtt gtggtccagc aaatcgcgta tggtctggac ctgcggcagg atcagccggt
     1141 ttttaggtgc ctggatgtcc tggggggtca ccaggattac ctgtgatttc ggcttcaaga
     1201 tatctccgat caaggcaggg agttcgaaat ttgagggggc atattggctt atcatttcct
     1261 ttagtttccc gatatttgtt ccctctttgg cgctgacctt aacaaatgga aagtcgaagt
     1321 ctttgctgta cttgtcaatg ccggtatctt ttatatcgac tttgtttaca acgccgatta
     1381 tggggatttt cctttcccgg agtttctcat accactcttt ttctaaatca aaactcgagt
     1441 tttccggtga gaagaccatc agtgccaagt ccgttttgtc catgacggcc agggtctttt
     1501 tgaccctaag ccttcccaaa tcgccggtat catccaaacc tgcagtgtct ataaacacga
     1561 cagggcccag cggtaaaagt tccatggcct tatagaccgg gtctgtagtt gtccccggat
     1621 gcttggaaac cagggccact tcctggcccg taattgcatt tatgaggctg gattttccgg
     1681 catttcttct gccgaatacg gctatgtgaa gccggtttgc cctaggtgtt tcattcatgt
     1741 catcacaacc tttcaaggag cctgagggag tcacctttgt cccttgctac ttcttttccc
     1801 atgctcttta aatactcttc catttcccgc cgctcttccc ttatgctctg ttcttcgcca
     1861 aagtccttgg gataaatctc gtacaacctc cggtatttgt aaggttctag ttttaacatt
     1921 attacatttg ctccaacgtg aaatgccgat tcggcgcctt gtccgcctaa ggtccttaga
     1981 gccgtagttg ccggcaaatg ggcggtcttt acgattagcc tggtaacagc caaagctttg
     2041 agagtcatct cgttgctgcc tgccggtgca tcccgaaggg ccgtcccctg atgggggata
     2101 aaggggccga ttcccgccat atccacttcc agcctgttta aaaacaaaat gtcctttgct
     2161 atgtcccgtg tggtctggcc gggaaggcct atcatgaatc cgctgccggt ctggtatccg
     2221 agcgttttta accgtttcag acacagaatc ctgtttgcca gcgttgagtg aggatgtaag
     2281 cttttataga gcctcggatt aatcgtctca tgctttaata aaaatctatc ggctccatcc
     2341 tctttccact gccggtaatc ctcataagct ctttcgccta cgctcaaagt tatggccaga
     2401 ccaccgatgg ccttgatttc ccttattatg taggatattt tttccttgct gtaccacaaa
     2461 tcttcacccg actgaagcac tatggtcttg tagcctgcat cataagcctt ttttgcaact
     2521 tcgattattt catcgggctc catcctgtaa cgtttaagtt ctttgttgcc cgcgttcaag
     2581 ccgcaatagg cacagcttgc cctgcagtaa tttgaaaact ctattatacc ccttatgtgg
     2641 acggtgtttc cgttgtagaa tctgtttatc tcatccgcag ctttgaaaat gtaatctagg
     2701 tcctgccctt cggcacaaag cagcttttcc acttcatcta caccgggatg gtatcctcca
     2761 catatcttct gcacaagttg gtagatttcc ataaagccct cctcataaaa aaaccttcgg
     2821 caaaaagccg aaggttacca ttcttgttcc atatttcact atcccggaca aaaaatatac
     2881 gtccaggcat aaaatgctcc ctctggtcct tcgccccttt gctcagaaca aatccttgca
     2941 acaaggctcc tatggacttt tgtatcctcc gcatcggaac ttccaatacc tcggatttag
     3001 tttccatgta tattatatac ctgaatgtaa cattgtcaat agtaaattgt ctcacaatac
     3061 catcggatta cggcgccagc ttttgcgcct cctctgccaa ggtaatacta ttgttgatct
     3121 tctccgccac ttcagctgtc aatatttcat catctgtatg cccttgtcgc ttaaaatacc
     3181 tgccatctgc atctccttta ctgcctccct ggcataggca ctgatttcgg tactgtctgc
     3241 gaaggtgttt tcttcatatt tatggtttag cttaagattc atagctttcg caaagttgct
     3301 catgattact gctaactctt cacgggtgat ggcttggtcc ggaggaaact tcttatcacc
     3361 gattcccttt acaatgccat tttcgcttgc ccactcgatg tagcccatgt agtaagcatc
     3421 atcttttaca tcggtatatc tcattattag tcagatatgc catgttccta ataattctag
     3481 tgcctattac aaaagcccta
//
LOCUS       DAONUO010000223         3484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18705_length_3484_cov_3.575970, whole genome shotgun sequence.
ACCESSION   DAONUO010000223 DAONUO010000000
VERSION     DAONUO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18705_length_3484_cov_3.575970"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK768_08050"
     CDS             <1..454
                     /locus_tag="PK768_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HQA60963.1"
                     /translation="GVLETTSGLEGIQITQAAVTSQVGAWGSTFVAISILLFSFSSII
                     GNYYYGETNIEFIKEDATFLTIYRLMVLAMVFFGSIGSFGLVWDTADVFMGIMAVVNL
                     IVITKLGPIAYATFDDYVSQLQEGKNPVFNPDKIPGLGKVECWGREKS"
     gene            complement(513..1079)
                     /locus_tag="PK768_08055"
     CDS             complement(513..1079)
                     /locus_tag="PK768_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQA60964.1"
                     /translation="MIYLQLLISFLKIGLFSFGGGYAMIPLIQREIQSHGWLSPSEFV
                     DIIAIAEMTPGPIAVNSATFVGYKAAGILGSLVATFGVAIPSLVLIMIVSKFFLKFQN
                     HPANKMVFYGIRPIIVGLIFASAIFVAQTALIKQGASNGSLFTNSLSVLDLKSILIFV
                     LSLIGLVRFKIHPIVIILISSILGLLLT"
     gene            complement(1076..1669)
                     /locus_tag="PK768_08060"
     CDS             complement(1076..1669)
                     /locus_tag="PK768_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQA60965.1"
                     /translation="MKKNNQFKLLMELFFTFFKIGSFTFGGGYAMIPLIEREVVEKKR
                     WVSEEEVVDVFAVAQSMPGAIAINSSTFIGYKIAGKRGAVVATLGVVLPSFFIIALIA
                     IFYSKFQDAAAVKAVFSGIRPAIVSLIAVAAIRISKTSIVDWTGLILAILSSFSVVFF
                     DVHAAFAILGGATAGLAIFIMFPKKTEKILSNGGNKN"
     gene            complement(1702..2895)
                     /locus_tag="PK768_08065"
     CDS             complement(1702..2895)
                     /locus_tag="PK768_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQA60966.1"
                     /translation="MTDGYSILKNIRDQSKLLLRIIQQKGPITRNQIIDMTRMKLTTL
                     KRFMQPLLDEGFVIEADLAKSNGGRPPVLYDVNPKTYYSIGIDISRTYTEVVITNLKM
                     DIIDINRFGPPQEPVDTVNQICSYLENRLIQLSIEKSMVLGVGVGTVGPLDRKKGIML
                     NPANFPSNIWVNIPIKDMIQQRLNLPTVVDNGANAAVLAEHLFGIGLDKNNISYFHCG
                     MGIRTGTISSGSIIRAINDYEDAFGHMVIDINGDLCHCHNRGCIETYCSIPSITKKFK
                     ELLKQGTPTYVDKSFDKIDFLDICAAAKENDILARQVITKAAEIFGIGLANYINLVTP
                     ELVILSGPIVKHSELFYNTAVEEALKRKYVKNHKVSFSRGGNFKDKAIAVGAAVLSVD
                     EILNN"
     gene            complement(2998..>3484)
                     /locus_tag="PK768_08070"
     CDS             complement(2998..>3484)
                     /locus_tag="PK768_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQA60967.1"
                     /translation="IGYIGEIPELGDYKVINEHRFRAFKNTLAKHNISLDENYIKNIT
                     LSSKEGYNAMTEIIKGGNLPSAIFCGNDITAIGVMKAIHKSGLKIPKDISVIGLDNIE
                     VSEYFRPSLTTVHVPKEELGRFAVKLLIDRINGGHEINTLIKLPFKLIERESCDISRN
                     L"
BASE COUNT         1081 a          694 c          631 g         1078 t
ORIGIN      
        1 cggcgtatta gaaactacct ccggtttaga aggcatacag atcacccaag ctgccgttac
       61 atcccaagta ggagcatggg gcagcacttt tgtggcaatt agtattttgc tgttctcttt
      121 tagctccata ataggaaatt attattatgg tgagacaaat attgaattca taaaagaaga
      181 tgctacgttt cttactatat atagactcat ggtgctggcc atggtcttct tcggctcaat
      241 cggaagcttt ggccttgtat gggacacagc agacgtattt atgggcataa tggcagttgt
      301 aaacttaata gtcataacca agcttggccc aattgcctat gccacctttg atgactatgt
      361 atcacagctg caggagggca aaaacccggt tttcaatcct gataagattc ccggtttggg
      421 caaagtggag tgttggggaa gagaaaaaag ctaatagcta gttaggagac taatgataaa
      481 agccccgggt ttttgacctg gggctttcgc ctttaagtga gtaagagtcc caaaatactt
      541 gaaatcagga ttatcacaat tggatgtatc ttaaatctga ccagacctat caatgaaagg
      601 acaaagatta ggatactttt taaatcaagt acagaaagcg aattggtaaa tagactgcca
      661 tttgaggctc cctgttttat taaagcagtt tgcgcgacaa aaattgcaga tgcaaaaatc
      721 aggccaacga tgatgggcct tattccgtaa aacaccatct tattcgcggg atgattttgg
      781 aattttaaaa agaacttcga aacaatcatg atcaggacaa gagagggaat tgctacgcca
      841 aaagtggcca ccagactgcc gaggatgccc gctgccttat aacccacaaa agttgcggaa
      901 tttacagcaa tgggccctgg agtcatctca gctatagcga ttatatccac aaactccgat
      961 ggagacagcc aaccgtgact ttggatttcc ctttgtataa gcggtatcat ggcatagccc
     1021 ccgccaaagc taaaaagtcc gattttcaaa aaggatatga gaagctgtaa gtaaatcaat
     1081 ttttattgcc tccgttgctt aaaatctttt ctgtcttttt tggaaacata atgaatattg
     1141 caaggcctgc agtagcaccg cctaaaattg caaatgcggc atggacatca aagaacacaa
     1201 ctgaaaaaga agacaagatt gcaagtatta aacctgtcca atctacaatt gaagttttcg
     1261 aaatccttat tgctgctaca gcaatgagag atactatggc gggccttatc cccgaaaaca
     1321 cagccttaac agcagctgca tcttgaaact tcgaatagaa aatagcaatc aaagctatga
     1381 tgaaaaatga tggcaataca actcctaagg tggcaacaac tgctcccctt ttacctgcaa
     1441 tcttataacc tataaatgtg gaggagttta ttgcaatagc gccgggcatg gattgggcta
     1501 cagcaaacac gtcaaccact tcctcctctg aaacccaacg ctttttttcc accacttctc
     1561 tttctattag gggaatcatg gcgtagcccc cgccaaaagt gaaacttcct atcttaaaaa
     1621 atgtaaaaaa aagttccata agtagcttaa attgattgtt ctttttcatt tggaacctcc
     1681 tcatatgtag ctttacatat atcagttgtt taaaatttca tcaactgaca ataccgctgc
     1741 tcctactgct atggctttat ctttaaaatt cccaccccta ctaaaggaaa ccttatggtt
     1801 tttaacatac ttgcgcttca aagcttcttc tacagctgtg ttgtaaaaaa gttcagagtg
     1861 ttttactatt ggcccgctga gaatcaccaa ttctggagtc actagattta tataattggc
     1921 aaggcctatg ccaaaaattt cagcggcctt tgtaattact tgccgggcta gaatatcatt
     1981 ttctttggcg gcagcacaga tatctaaaaa atctatttta tcaaaggatt tatcaacata
     2041 ggtaggggtg ccctgcttaa gcaactcttt gaacttttta gtaatagaag gtatggagca
     2101 atatgtttca atacagccgc gattatggca gtggcataaa tctccattta tatcaattac
     2161 catgtggcca aaagcatctt cataatcgtt aattgccctt ataatgcttc ctgatgaaat
     2221 tgtgcctgtt ctaataccca taccgcagtg aaaatacgat atattatttt tgtctaagcc
     2281 tattccaaag agatgctcag ctaaaacggc agcatttgca ccattatcga ctacagttgg
     2341 gagatttaat ctttgctgta tcatgtcctt gataggaata ttaacccata tatttgaagg
     2401 aaagttggct ggatttaaca taataccttt tttacggtct aaaggtccaa ctgtgcccac
     2461 accaactcct aagaccatag atttttctat agacaactga attaacctgt tctcaagata
     2521 tgaacatatt tgatttacag tatctacagg ctcctgaggt ggtccgaagc gatttatatc
     2581 gatgatatcc atttttagat ttgtaatgac aacttcggta taagttcgag aaatatcgat
     2641 tccaatacta tagtaagtct ttggatttac atcatatagt acagggggtc ttcctccatt
     2701 agacttagcc aaatctgctt caataacaaa gccttcatct agcagaggct gcataaagcg
     2761 tttaagtgta gtcaacttca tccttgtcat atctattatt tgatttcttg taattggacc
     2821 cttttgctga ataatacgta gcagcaattt actctggtct cttatatttt ttaatatact
     2881 gtagccatca gtcaccaata tctcctccaa actggaagac tttttaataa catgataaca
     2941 ccttttttcc aaaatggcaa aaagtctaca aaaaaagaat tcaactaaaa atagaaacta
     3001 taaatttctg gatatgtcac agctttctct ttcgattaat ttaaaaggga gtttaataag
     3061 agtatttatt tcgtgtccgc cgtttattct gtcaattaat aatttgacag caaatctccc
     3121 caactcttct tttggaacat gaactgttgt taaagaagga cggaaatatt cagacacctc
     3181 aatattgtca aggcctataa cagaaatgtc ttttggaatt tttagaccgc ttttgtggat
     3241 tgccttcatt actccgatag cagttatatc atttccacaa aaaatagcgc tgggtagatt
     3301 tcctcctttg attatttcag tcatggcatt atagccttct ttggaagata atgtaatatt
     3361 tttaatataa ttttcatcta aagaaatatt gtgtttggcc aaggtgtttt taaaagctct
     3421 aaatctatgt tcatttatta ccttataatc tcctaattcg ggtatctcgc ctatgtagcc
     3481 aatt
//
LOCUS       DAONUO010000224         3466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18844_length_3466_cov_4.047200, whole genome shotgun sequence.
ACCESSION   DAONUO010000224 DAONUO010000000
VERSION     DAONUO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18844_length_3466_cov_4.047200"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="gpmI"
                     /locus_tag="PK768_08075"
     CDS             complement(<1..977)
                     /gene="gpmI"
                     /locus_tag="PK768_08075"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQA60968.1"
                     /translation="MSKKPLMLMILDGWGLNPETKENAIVNANTPNFNRLMKEFPNTT
                     LQASGLAVGLPEGQMGNSEVGHLNLGAGRIVYQDFTRVTQDIKNGNFFKNPALVWAMD
                     NALENNKSLHLLGLLSDGGVHSHIDHLKALLDMAKLKKLQKVYLHAFLDGRDVPPANA
                     IKYINEIEEYMEKIGVGKIATISGRYYAMDRDKRWDRTEKAYNAIVFGEGLKASSAVE
                     AVEQSYERKETDEFVVPTVVTNEKGEPVATVEPGDAIIFFNFRPDRARQLSYAFCNEE
                     FDGFSRKRGFFPVSYVCLTEYDITIKNARIAYGQESLDNILAEVLSAAGL"
     gene            complement(993..1748)
                     /gene="tpiA"
                     /locus_tag="PK768_08080"
     CDS             complement(993..1748)
                     /gene="tpiA"
                     /locus_tag="PK768_08080"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQA60969.1"
                     /translation="MSRIPLMAGNWKMNNDKRETLDFLSSFLPKVKELTVEIVICPPF
                     TSLYAAAEKLVGTNVKLGAQNMHWEEKGAFTGEVSPLMLKELPCDYVIIGHSERRQYF
                     AETDETVNNKIKSALKHELAPIVCVGETLEQREEGITIDFVMGQVEKALKGIEAEEAK
                     KIVFAYEPIWAIGTGKTASAADAQEVIGAIRNKISELYNKEVAEAVRILYGGSVKPEN
                     IKELMAEADIDGALVGGASLKAESFYAICSFNL"
     gene            complement(1804..2988)
                     /locus_tag="PK768_08085"
     CDS             complement(1804..2988)
                     /locus_tag="PK768_08085"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQA60970.1"
                     /translation="MNKKTLQDFNLENKRVLVREDLNVPLDENQNITDDTRIRAALPT
                     INYLIEKGAKVIIVSHLGRPKGKVNPKYSLAPVAKRLSELLGRDVAFAKDCIGEAAQE
                     AVDKMKAADVLLLENVRFHEGEEKNDPDFAKQLARLADIFINDAFGTSHRAHASTVGV
                     ADFLPAGAGFLLQKEIEVMGKALENPERPFVAILGGAKVGDKIGVIKNLLNKVDTLLI
                     GGGMAYTFLKSKGYEIGQSLLEEDKIDLAASLLKEAEEKNVKLLLPEDVVVTSELKEG
                     LPHETVPVSEIPEDKMGVDIGEETRKKFAEAIKEAKTVVWNGPMGVFEIREYAQGTLA
                     VADAMAKSEAVTIIGGGDSAAAVEQLGFAEAMTHISTGGGASLEFLEGKELPGIAALD
                     DR"
     gene            complement(2988..>3466)
                     /locus_tag="PK768_08090"
     CDS             complement(2988..>3466)
                     /locus_tag="PK768_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HQA60971.1"
                     /translation="NTIAAELAKKLSAQYRLLYVPDNLSQETMESIVNEPGIREILKI
                     VRRANILLHGIGGAEEIGRRRGLSEQEISKIRKKGAVAEAFGYYFNKEGKVVHTASSV
                     GLSVTDLKNIRIVIAIAGGANKAPAIRAFLKYHSPTVFITDEGAARRLLKLEGGEN"
BASE COUNT          798 a          904 c          687 g         1077 t
ORIGIN      
        1 agcccggcag cagataacac ctctgccaga atattatcta gggattcttg cccatatgct
       61 attcttgcat tttttattgt gatatcatat tcggtcaggc atacgtaact gacggggaaa
      121 aatcccctct tgcgggaaaa gccgtcaaat tcctcgttac aaaaagcata gctaagctgt
      181 ctagccctgt caggccggaa gttaaagaaa ataattgcat ctcccggctc gacggtggct
      241 acaggctcgc ccttttcatt agtaactaca gtcggcacca caaactcatc agtttccttg
      301 cgctcatagc tttgctccac tgcctctaca gcacttgatg ctttaagacc ttcaccaaag
      361 actatcgcat tataggcttt ttccgtccta tcccagcgct tatccctgtc cattgcatag
      421 tagcggccgg aaattgtcgc tattttgcct acaccgattt tttccatgta ttcttctatt
      481 tcattgatgt attttatggc attggccgga ggcacgtctc tcccgtctaa aaaagcatgt
      541 aagtaaacct tttggagctt tttcagttta gccatatcga ggagtgcctt gagatggtct
      601 atatggctgt gaactccgcc atcggacaaa agccccaaaa gatgaaggga tttattgttc
      661 tctaaagcat tgtccattgc ccaaactaat gcaggatttt taaagaaatt accgttcttt
      721 atatcctggg tcaccctagt gaagtcttga tatacgattc gacctgcacc tagatttagg
      781 tgtcccactt cggagtttcc catttggcct tcaggcagtc ctaccgcaag acctgatgct
      841 tgtaaagtag tgtttggaaa ctctttcatg agcctgttaa aattgggtgt gttagcattt
      901 acaatggcat tttcttttgt ttccgggtta agcccccaac cgtctaatat cattagcatc
      961 agcggttttt ttgacacttt cttcaactcc tattatagat tgaaagaaca aatcgcataa
     1021 aagctttcgg ctttaaggct ggcgccaccc accaaggccc catcgatatc ggcttctgcc
     1081 attagttctt tgatgttttc cggctttaca ctgccaccgt atagtattct cactgcttca
     1141 gcaacctctt tgttgtaaag ctcacttatc ttgtttctaa tagcaccaat tacctcctgt
     1201 gcgtcagctg ctgaagctgt tttgcccgtg ccaatggccc agatgggctc gtaggcaaaa
     1261 actatttttt tggcctcttc ggcttcaata cctttcagcg ccttttccac ttggcccatt
     1321 acaaaatcta tcgttatgcc ctcttcccgt tgctccaagg tctctccgac acatacgatt
     1381 ggagcgagtt catgctttag ggcagacttg attttgttgt tcaccgtctc atccgtttcc
     1441 gcaaaatact gccgccgctc tgaatgccca ataattacat aatcgcatgg aagctctttg
     1501 agcataaggg gcgagacttc ccctgtaaag gccccctttt cttcccagtg catattctgg
     1561 gctcccagtt ttacatttgt gcctaccagc ttctcagctg ccgcatatag ggaagtaaaa
     1621 ggcgggcaga taactatttc tacggtgagc tctttcacct ttggcaaaaa acttgagaga
     1681 aaatccaagg tttctctttt atcattgttc attttccagt taccagccat caaaggaatt
     1741 cttgacatca tgaatacccc cgatttcatt attttatatc aaatttaagg aaaaatccac
     1801 acatcacctg tcatctaaag ctgcaatacc gggcagctcc ttgccttcca ggaattccaa
     1861 ggacgccccg cctccggttg atatatgagt catggcctct gcaaaaccca attgctcaac
     1921 tgccgctgcc gaatcgccgc cgccgattat ggttactgct tcggattttg ccatcgcatc
     1981 ggctaccgct aatgtgcctt gtgcatattc ccttatttca aatactccca tgggaccatt
     2041 ccacactacg gttttggcct ccttaattgc ctccgcaaat ttctttcgag tctcctcacc
     2101 gatgtcaaca cccatcttgt cctccggaat ctccgatacg gggaccgtct cgtgaggcag
     2161 gccttcctta agctctgaag ttacgacaac atcctccggc agaagtaact ttacgttctt
     2221 ttcttcggct tctttaagta gagatgcagc taaatcaatt ttatcctctt caagcagaga
     2281 ctgacctatt tcgtatccct tgctctttaa gaaggtatag gccatgccgc caccgattag
     2341 cagcgtgtca actttgttaa gcagattttt aatgactcca attttatccc caacttttgc
     2401 accgcctaaa attgccacaa agggcctctc cggattttcc aaggccttac ccataacttc
     2461 gatttccttc tgcagtaaaa atcccgcacc tgccggcaag aagtctgcta cacctactgt
     2521 agaagcatga gccctgtgcg aggtgccgaa ggcatcgttt atgaaaatat cggcgagcct
     2581 tgcaagctgc ttggcaaagt caggatcatt tttttcttct ccttcgtgaa atcttacatt
     2641 ttcaaggagc agcacgtcag ccgctttcat cttatctact gcctcttgtg cggcttcacc
     2701 tatgcagtct tttgcaaaag ccacatccct gccgagaagt tcggaaagcc ttttggccac
     2761 cggagcaagg ctgtactttg gatttacttt accctttggc ctaccaaggt gtgaaactat
     2821 gataaccttg gcgccttttt caataagata attaattgtc ggcagtgctg ccctgatgcg
     2881 cgtatcatcg gttatgtttt ggttttcatc taatggcaca tttaggtctt ccctgactaa
     2941 gacccttttg ttttccaggt taaaatcttg aagagttttc ttgttcatca gttttcgcct
     3001 ccctcaagtt ttagcaatct tcgggccgct ccctcgtcgg ttataaaaac tgtagggctg
     3061 tggtatttaa gaaaggcccg tattgccggt gccttgttgg ccccaccggc tatagcaatg
     3121 actatccgaa tgttttttaa atccgtgacg ctgaggccca cactgctggc agtatggaca
     3181 accttgcctt ctttattaaa gtaatatcca aaggcttcag ccacggcacc ttttttgcgt
     3241 attttgctta tttcctgctc tgaaaggcca cgtcttcggc ctatctcctc cgctccccct
     3301 atgccgtgaa gcaagatatt ggccctgcgg acgatcttca gtatttcacg gattccgggt
     3361 tcatttacta tggattccat ggtttcctgg ctcaggttgt cggggacgta aaggagcctg
     3421 tattgggcac ttagtttttt ggcaagttcg gcagctattg tatttg
//
LOCUS       DAONUO010000225         3462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18866_length_3462_cov_3.343704, whole genome shotgun sequence.
ACCESSION   DAONUO010000225 DAONUO010000000
VERSION     DAONUO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18866_length_3462_cov_3.343704"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK768_08095"
     CDS             complement(<1..343)
                     /locus_tag="PK768_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HQA60972.1"
                     /translation="MLENGKISNKQMILIFVICRLMLTMAYLPYVNSPPWNQDLWISA
                     ILSFPLHIILILPVYALAMRFSKLSLIQSIEVILGPCGKILGAMYVWFFLHRTSIILR
                     ELGEFLTAVPYP"
     gene            complement(371..1111)
                     /locus_tag="PK768_08100"
     CDS             complement(371..1111)
                     /locus_tag="PK768_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HQA60973.1"
                     /translation="MDGSPFVLTVPYLFIEAFQSSEDYYSRPFYSSIVRLLRFTAYFI
                     SVFSPAVYVALTTYHQEMLPPAMLYSLAAAREGTPFPAFVEAMMMGIIYEILKEAGIR
                     LPRPVGQAVSIVGALVIGESAVSAGLIGAPMVIVVSLTAISSYVVASISDTITIMRLT
                     YVLLAAFMGLFGIMIGAAGFLTHMVSLRSFGVPYLAPLAPIVTEDLKDVFFRPHLWSE
                     CKRPKLIAKENIVRVKSDSMPKPPENNS"
     gene            1230..2219
                     /locus_tag="PK768_08105"
     CDS             1230..2219
                     /locus_tag="PK768_08105"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HQA60974.1"
                     /translation="MLYIDNKSNDPYYNMALDEYVVKYLDASKDYFYFYQNRPTVVIG
                     RNQNTIEEVNLDYVKKHDIIVVRRMSGGGAVYHDMGNINFSFVVDYRKEDFNSMERFA
                     KAIVKALSKFGINPEFSGRNDITIDGKKFSGNAQYVTRNRILHHGTLLFDSDLTVLSK
                     ALNVKPEKIRSKGIKSVKSRVANIKEYLPDKNVTVSQFKQMLLNYVFEVEGVELQEYR
                     LTPEDMEQIMRLRDEKYSTWEWNVGSSPEFDLIRSTRFSGGEVQVHMNVRDGIITDIR
                     FYGDFMSIRDVSELEEKLKGQKFKQGDIKEVLSQIDIKEYFGFISLEELLSLF"
     gene            complement(2345..2815)
                     /locus_tag="PK768_08110"
     CDS             complement(2345..2815)
                     /locus_tag="PK768_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HQA60975.1"
                     /translation="MENNAGFYTYRGYELLRQEKLRLSPSMEDYLEMIYRTCKKQGYI
                     RMTSLAQQLNVQASSATKTVQKLAEMKLLAYEKYGIIQLTEEGRKIGEFLLKRHRIVE
                     TFLKNIGVKENLLRQTEMIEHHLTADTVRSIDMLNKFFKEYPEIHKQFLQFLIN"
     gene            2961..3200
                     /locus_tag="PK768_08115"
     CDS             2961..3200
                     /locus_tag="PK768_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQA60976.1"
                     /translation="MEENAISLCDLPIGSFGRVKDIAAKGSTRRRMRDLGLVDNTLVE
                     VLRKSPSGDPVAYEIRGAVIALRFEEASKVFVKPC"
     gene            3218..>3462
                     /locus_tag="PK768_08120"
     CDS             3218..>3462
                     /locus_tag="PK768_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HQA60977.1"
                     /translation="MGLTGQSTWLGVLKNKSCNLKRDSTSVVVALAGNPNTGKSTVFN
                     SITGLNQHTGNWPGKTVSSAQGTYEYKGESFLLVDLPG"
BASE COUNT         1080 a          681 c          764 g          937 t
ORIGIN      
        1 cggggtaggg gacggcggtg agaaattccc ccagttccct taagataatt gatgttcgat
       61 gtagaaaaaa ccatacgtac atagcaccga gaattttccc gcagggaccc aaaatcacct
      121 ctatggattg tatcagtgac agcttcgaaa agcgcatagc aagcgcatat accggcagta
      181 tcagtattat gtgaagggga aatgatagga ttgccgaaat ccacaaatcc tggttccaag
      241 gtggagaatt cacatacggc aaatatgcca ttgtaagcat gaggcgacaa atgacaaaaa
      301 tcagtatcat ctgtttattt gatatctttc cattttccag cataaagtac ctcataaaac
      361 tcattcgttt ttagctattg ttttcgggag gtttgggcat tgaatcagat ttaacacgga
      421 ctatattttc tttggctata agcttgggcc ttttgcattc actccacaag tggggacgga
      481 aaaacacatc cttcaaatcc tccgtgacga taggggcaag gggagcaagg tacggcactc
      541 caaaggaacg gagggatacc atgtgggtta aaaagcctgc cgcaccgatc ataatgccaa
      601 acaggcccat aaaagctgcc aacaagacat aggtaaggcg cattatggta atggtatcgc
      661 ttatggaggc aaccacatat gaggatatgg cagttaaaga caccacaatg accatgggag
      721 ctcctatgag acctgccgaa actgccgact cacctattac taaggcccct actatgctta
      781 ctgcctgacc tactggcctt ggtagcctaa tacctgcctc ttttaaaatc tcatatatga
      841 ttcccatcat cattgcttct acaaaagcgg ggaagggggt accctctctg gctgcagcca
      901 agctatagag catggccggt ggcaacattt cctgatggta tgtggtcaac gctacgtata
      961 ctgctggtga aaatacactt ataaaatagg cggtaaatct caatagcctt acaattgacg
     1021 agtaaaaagg ccgtgaataa tagtcttcac tgctttgaaa agcttctata aacaaatagg
     1081 ggacagttag cacaaaaggg cttccgtcaa tagctattga tatagttgca aagcctcttc
     1141 cctagctgat ataatttaaa atatcattta tttaaaacac ggtgaaattc caaatgttgt
     1201 ggatattgat taaggaggtg aaatgtaaca tgctttacat agataataaa tccaatgacc
     1261 cgtattataa catggcctta gatgaatatg tggtaaagta cctggatgca tcgaaggatt
     1321 atttttactt ttatcaaaac aggcctactg tggtcatcgg aagaaaccaa aacaccatag
     1381 aagaagtaaa tttagactat gttaaaaaac acgacataat agtcgtcaga aggatgtccg
     1441 gcggcggagc agtgtaccat gacatgggga acataaactt ttcctttgtg gtagactacc
     1501 ggaaagaaga tttcaacagc atggagagat ttgccaaggc tatagttaag gccctaagta
     1561 aattcgggat aaacccagag ttttcaggaa gaaatgacat caccatagat ggaaaaaaat
     1621 tttccggcaa tgcccagtac gtcacaagaa accggattct tcatcacggg accctgctct
     1681 ttgactctga tttgacggtg ctgtccaagg cattaaatgt gaagccggaa aaaatcaggt
     1741 caaagggcat aaaatcagtt aaaagtagag ttgcgaacat taaagaatat ttgcctgaca
     1801 aaaacgttac cgtatcccaa tttaagcaaa tgctactgaa ctatgtcttt gaagtggaag
     1861 gagtcgagct tcaggaatat cgtcttacac cggaagacat ggaacaaata atgcgcctga
     1921 gagatgaaaa gtactcaaca tgggaatgga acgtgggcag ttcaccggaa tttgacttaa
     1981 tccgctctac ccgctttagc ggtggggaag tccaggttca tatgaatgtc agggacggaa
     2041 ttataactga cataagattt tatggtgatt tcatgagtat tcgggatgtt tccgaactag
     2101 aggaaaaact taaaggccaa aaatttaaac aaggtgatat caaagaggtc ctatcccaaa
     2161 tagatataaa ggaatatttt ggctttatta gtttagaaga gctcctctcg cttttttaat
     2221 attagcaaac cggatgattt atagtacttc caaagtagat agtctaatca aaataagaca
     2281 tctgctggga agtatttttt ttggagaaaa gaaaaagttc ctagcgaatt atataaacac
     2341 ttgcctagtt gattagaaat tgaagaaact gtttatgaat ttccggatat tctttaaaaa
     2401 acttattcag catatcaata cttcttacag tgtctgcggt taagtgatgc tcaatcatct
     2461 cggtttggcg aagaaggttc tcctttaccc ctatgttttt caaaaatgtc tcgacaattc
     2521 ggtgcctctt taaaagaaat tctccaatct tcctaccctc ttcagtaagc tgtataatgc
     2581 cgtatttttc ataggccaag agtttcattt ctgcaagttt ttgcaccgtc tttgtggccg
     2641 acgaggcctg tacgtttagc tgttgagcca gacttgtcat tcttatgtat ccttgttttt
     2701 tacatgtcct gtatatcatt tcaaggtaat cttccatgct cggcgaaagg cgaagctttt
     2761 cctgcctcag caattcgtac cccctgtaag tgtaaaagcc cgcattgttt tccacttgtc
     2821 aacctccaaa ctagttgtaa cagtacttaa aatgaaaaca tatattattg ttagcagcat
     2881 aaaagtttcc ctcgtctaag ttatttttaa gggacgaaaa ttatgcaccg aatgtcaatg
     2941 atatcaaggg ggttttgaaa gtggaagaaa atgcgatttc cttgtgcgat ttgccaatag
     3001 gcagctttgg aagggtaaaa gatattgcag ccaaaggcag taccaggcgc cgaatgcgag
     3061 accttggcct tgttgacaat acacttgttg aagtcctgcg aaaaagccca tctggagacc
     3121 ccgttgccta tgaaataagg ggagcggtca tagctttaag atttgaagag gcttcaaagg
     3181 tgttcgtcaa gccttgctga aggggaggcg ttggcagatg ggcctcaccg gtcaatcaac
     3241 atggcttgga gttttgaaga acaaaagctg taacttaaaa agggatagta ccagcgtggt
     3301 ggtagccctt gcaggcaatc ccaataccgg gaaaagtaca gttttcaaca gcataacggg
     3361 cttaaaccag cacaccggca actggccggg taaaaccgta agcagtgccc agggcactta
     3421 tgaatataaa ggtgagagtt ttttgctggt ggatttgcca gg
//
LOCUS       DAONUO010000226         3450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18952_length_3450_cov_3.454492, whole genome shotgun sequence.
ACCESSION   DAONUO010000226 DAONUO010000000
VERSION     DAONUO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18952_length_3450_cov_3.454492"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..568)
                     /gene="purE"
                     /locus_tag="PK768_08125"
     CDS             complement(50..568)
                     /gene="purE"
                     /locus_tag="PK768_08125"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQA60978.1"
                     /translation="MKKNKTLVGIIMGSDSDLPVMQEAAKVLEEFDVNYEITIVSAHR
                     TPDRMFEYAKTAKSRGIEVIIAGAGGAAHLPGMVAAITPLPVIGVPVKTSSLNGLDSL
                     LSIVQMPGGVPVATVAINNAKNAGLLALQILGIKYPEIGDKIQQYKDNMKDEVLAKAK
                     RLESTGYRDYSK"
     gene            complement(570..1712)
                     /locus_tag="PK768_08130"
     CDS             complement(570..1712)
                     /locus_tag="PK768_08130"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQA60979.1"
                     /translation="MSCPMDFKIGIIGGGQLGKMLAQEAKKLNLPVTVLDPTPQCPAY
                     SLVDRQIVAGFYDEVKIRELVTSSDITTFEIEHISTKVLKELQAMGHKIHPSPEVLEI
                     IQDKAKQKQMLDKNGIPTARWALVESDIAEAARKFGLPAVQKACKGGYDGRGVFVIQN
                     PEDLSDALQCESFLEELIPVEKELAVMVARNIKGEVKCYPVVEMTFNHKANICDDTIA
                     PARVEESIKKEAEDLAIRCVKALDGVGIFGVEMFLTKNNRVLVNEIAPRPHNSGHYTI
                     EACVTSQFEQHLRAILGLPLGSTELVMPAVMVNLLGAEGYQGKPCFEGIDEVLSIPGV
                     SVHIYGKKETKPYRKMGHITVVDKNIENALEKAEIVKKTIRVVSEG"
     gene            1936..2787
                     /locus_tag="PK768_08135"
     CDS             1936..2787
                     /locus_tag="PK768_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HQA60980.1"
                     /translation="MQKHRGDTMRAMWRGSISFGLINVPIKMYAATENKRISFRQIHR
                     ECGTPIHYEKVCTTCNRKVEDDEIARGYEFEKGRYVIIEDKDLESIPDETTRTIDIID
                     FVELADIDPIYYDRSYYLGPEETGQKAYILLRRAMEEGGKIAIAKVVIRSKQSLACIR
                     PYGKGHLVMETMFYPDEVRNVEDVPVQPQAKLQEREIEMALQLVESLSTRFEPEKYTD
                     DYRQALIEIIQAKVEGEEISIPTPRDEKVVDLMEALKASLELAEKAKKQKGKARAKTK
                     EKVAGGV"
     gene            complement(2788..>3450)
                     /locus_tag="PK768_08140"
     CDS             complement(2788..>3450)
                     /locus_tag="PK768_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HQA60981.1"
                     /translation="KPNFPEIIRLYCATDTGTIKHLAKLIPVTYVLFDLVFYQDKDLT
                     ACSFKYRDELLKSLIMSKDPIVVTDTIHNHGTRLFSVVKEAQLEGIVAKKLDSPYRIG
                     KKSPDWLKIKNFRTLAALVGGFIHEGRQARSLLLGAYQGTEFVYLGRAGSGLSREMAS
                     LLFEKLNKIKVSHCPFTVSLQINKKEQISWVKPSVEVIVEYMEFSDDGLLRHPVIKEV
                     II"
BASE COUNT          962 a          771 c          749 g          968 t
ORIGIN      
        1 ttacattgca acatggcaaa cgaaacgtcc ctctgtcagg ttcctggcct tatttagaat
       61 aatctcgata tccggtgctc tcaagccttt tggctttagc caatacctca tccttcatat
      121 tgtctttata ttgttgtatt ttatcgccaa tttcggggta cttgatgccc aggatttgaa
      181 gggcaagcaa gccggcgttt ttggcattgt ttatggctac cgtagctaca ggaacaccgc
      241 caggcatttg gacgatggaa aggagcgaat cgaggccgtt taaacttgaa gttttaaccg
      301 ggaccccgat tactggaaga ggggtaattg ccgccaccat tccgggcaga tgggcggcac
      361 cgcccgctcc ggctataatt acctcgatgc cccttgactt ggcagttttg gcgtattcga
      421 acattctatc aggggttctg tgggcagaaa ctatggttat ttcataattg acgtcaaatt
      481 cctctaaaac cttggctgct tcctgcatta cgggaaggtc agagtcactg cccatgataa
      541 ttcccacaag agtcttattc tttttcatat tatccctccg aaaccactct aattgttttc
      601 ttcactatct ctgccttttc caaagcattc tctatatttt tatcaactac agtaatatgg
      661 cccattttcc tatagggctt ggtctctttt tttccgtaaa tatgtacact gacacccgga
      721 atactcagca cctcgtcaat accttcaaaa cacggcttgc cttgatagcc ctcagctccc
      781 agcaggttta ccatgactgc aggcatcacg agttccgtgg agcccaaggg caaaccgagg
      841 atggccctta agtgctgttc aaattgagat gtcacacagg cttcaatggt gtaatggcct
      901 gaattgtggg gccttggtgc tatttcattg accagtaccc tattgttctt tgtcaaaaac
      961 atttctacac caaaaatacc cacaccgtct aaggccttga cacatctgat ggccaggtct
     1021 tcggcctctt tttttattga ttcttcaaca cgggccggag caatggtatc gtcgcatata
     1081 ttcgccttgt gattgaaggt catttccact acgggatagc attttacttc tcctttgatg
     1141 tttctggcaa ccatcaccgc cagttccttt tctacgggaa taagttcttc caggaaagac
     1201 tcacattgca gggcatcact caaatcttca ggattttgaa tgacaaaaac gccccggccg
     1261 tcatagccgc ccttgcaggc cttttggact gccggcaagc caaattttcg ggctgcctcg
     1321 gctatatcgc tttctacaag agcccagcgt gcggtaggaa tgccgttttt gtctagcatt
     1381 tgcttttgct tggccttgtc ctgtattatt tccaaaactt caggggaagg atgtattttg
     1441 tgtcccatgg cctgaagctc tttaagcacc ttggtgctta tatgttcaat ttcaaatgtc
     1501 gtaatgtcgc tgctcgttac aagctcgcga attttgacct catcgtaaaa tccggctact
     1561 atttgcctgt ccacaaggga gtatgcagga cactggggag tagggtccaa aactgttacc
     1621 ggtaaattaa gtttttttgc ctcctgggcc agcatttttc ccagctgacc tccgccgatt
     1681 atccctattt taaaatccat tggacatgac atcgagcgtt ttcctcccaa atctattctt
     1741 ttggaagtat tatatcacaa atttagacag attctataat aaaagcaaaa cctgttgata
     1801 actaactatc gataccatta aaagaagagt acctcgctag tcatcaacag gtaatattat
     1861 atttccccca aaaaataaag ttataataat aatttcatgg actatttacc aataatataa
     1921 aatagcagct ctattatgca aaagcacaga ggtgatacga tgcgtgccat gtggcggggc
     1981 tctatcagtt ttggcttaat aaatgtccca ataaaaatgt atgcggcaac cgagaataaa
     2041 aggataagtt ttcgacaaat acaccgggaa tgtgggacac ccattcatta cgaaaaagtg
     2101 tgcacgacat gtaatcgcaa ggttgaagat gacgagattg caagaggcta tgagtttgaa
     2161 aaggggcggt acgtaattat agaagataag gacttggaaa gtattcccga tgaaactaca
     2221 agaacgattg acataataga ttttgtggaa ttagcggata ttgaccccat atattacgac
     2281 agatcatatt atttagggcc ggaagaaacc ggtcaaaagg cgtacatctt gctcagaagg
     2341 gccatggaag agggaggcaa gatagctatt gccaaggtgg ttatacgttc aaaacagagc
     2401 ctagcctgta tcaggcctta cggtaaaggt cacctggtaa tggaaaccat gttttatccg
     2461 gatgaggtaa ggaatgtgga ggatgtaccg gtacaaccgc aggccaagct ccaggaaaga
     2521 gaaatagaga tggccctgca gcttgtagaa tccttgtcta cccgctttga accggaaaag
     2581 tataccgatg attacagaca ggcactcatc gaaataattc aggcaaaggt agaaggggaa
     2641 gaaataagta taccaactcc tcgggatgaa aaggttgtgg atttaatgga ggcattaaag
     2701 gcgagcttgg aattggctga aaaggccaag aagcaaaagg gcaaagccag ggccaagaca
     2761 aaggaaaaag ttgccggagg agtttgatta aataataact tctttaatga cgggatgccg
     2821 aagcaagccg tcatcgctaa actccatgta ttccactatg acttcaacac tcggcttgac
     2881 ccaggatatc tgctcttttt tatttatttg aagcgacaca gtaaagggac aatggcttac
     2941 ttttattttg tttagttttt caaacaacaa agaggccatc tcccgactca ggccgctacc
     3001 tgcccttccc aaatacacaa attccgtgcc ctggtaagca cccagtagca gcgacctggc
     3061 ttgtctgccc tcatggataa aacctcccac aagagcagca agcgtcctga aattttttat
     3121 tttgagccaa tccgggcttt ttttgccgat tcggtaaggg ctatccagct tttttgcaac
     3181 tatgccctca agctgggctt ccttgacaac ggaaaacagt ctagtgccat gattgtggat
     3241 agtatcagtt acaacaatgg ggtcttttga cataataagg gattttagaa gctcatcccg
     3301 gtatttaaag gagcatgcag taaggtcttt gtcctgataa aacacaaggt caaacaacac
     3361 atatgtcacc ggaatgagct ttgcaagatg ctttatagtg ccggtatctg tagcacaata
     3421 caaccttatt atctcaggga aattaggctt
//
LOCUS       DAONUO010000227         3446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_18984_length_3446_cov_3.564140, whole genome shotgun sequence.
ACCESSION   DAONUO010000227 DAONUO010000000
VERSION     DAONUO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18984_length_3446_cov_3.564140"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK768_08145"
     CDS             complement(<1..204)
                     /locus_tag="PK768_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60982.1"
                     /translation="MPFSLQHKSLLSKLLLLTLILSAVTLALPLSMFASEIINDDKQP
                     KIKVLCYHHIIPETLSKRIDTVVS"
     gene            489..1838
                     /locus_tag="PK768_08150"
     CDS             489..1838
                     /locus_tag="PK768_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA60983.1"
                     /translation="MDSIRQRIIDAQMAAQSLVIYRSILKDPIVETFMQILDLALGEA
                     SFQELVTKYHDFFSRLVRQSENRSEPAFGNLWQDHILNLVIGDENPFSLRCEKTGIAG
                     ISRDLITLTQRDLASLQKIHDFDFSIFTSYIGQKAGELVVGFPVMYSIESQRHIPYPE
                     SYFKEKYHVKKLMGNSTDWDRNIEVLAKFYFEKGCGIFARFWAFKWVCTENGGDLVGI
                     SEPDPIRLNDLIGYEEQKQQVLRNTRQFVQGFGANNMLLYGDRGTGKSSTIKALVHEY
                     GQEGLRMVEVTKDQLMSLPDIIAKFNNRPYRFIIFVDDLSFEEHETEYKYLKAVLEGS
                     LEPAPENVLIYATSNRRHLIREYHSDRVQDEVRAQDTLQEKLSLSDRFGITVIYPSPD
                     QEEYLKIVQGIAGSRGIDVEPEQLRQMALKWELWHNRRSGRTAKQFIEDLLGRLRKA"
     gene            1870..1995
                     /locus_tag="PK768_08155"
     CDS             1870..1995
                     /locus_tag="PK768_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60984.1"
                     /translation="MTGSGGMPSRDMEAFEIGLQEAMDSFKCEDDGKRKCVCRKK"
     gene            2230..>3446
                     /locus_tag="PK768_08160"
     CDS             2230..>3446
                     /locus_tag="PK768_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQA60985.1"
                     /translation="MAHTDQIIDLIDEGIIAVDSRGYITTYNKIARDFFGINLTDGPG
                     HRDGKCVEGDWVLIADNGLGEDDGGMKAQDFKVIGVDPVDIMEGDSIVAIGRKGGLLG
                     EGIYKRLEKHTAQKELFVETYINGIKLRSMINYDLKLLRITVGEQNFDYIYLWSAGHM
                     VIVDGKNMQVKFYQSRGYTARNEDMKTILYGGHFMGKGIYGKSIDVEHMHISELHPDS
                     DIVKKLTDVALGQDCSIRGMETSINGIPVRCSIEPLNKDGKRVGALLKLTDITEIKAL
                     WHEREKALLTLETLENKLKTFHIKEEAFKDIVGNSESIKYAVDLAKRAADTSSTVLLL
                     GESGTGKGVFAEAIHRASSRRDKPFVYVNCASIPEALIESELFGHERGAFTGAVSEKK
                     GKFEIADGGTIFL"
BASE COUNT         1052 a          631 c          838 g          925 t
ORIGIN      
        1 actgacaaca gtgtctattc tttttgataa ggtttcaggg atgatatggt gatagcacaa
       61 gacttttatc ttgggctgct tgtcatcatt tatgatttcc gaggcaaaca tgcttagggg
      121 cagggctaaa gtgactgcag ataatatcaa tgtcagcaaa agcagcttac taagcaagga
      181 tttatgctga agggaaaagg gcaaatcgta caactccttt cagttgttta agttctaaga
      241 aacaatttat agaattatag taacatatag gtaatactgt gtaaaccagg caaaagcata
      301 cattaattac aaaaacggct ttttttgata taatcaagct aaaaagcctt gctatttttg
      361 tttttgagca aaggggccaa gtgcggagca aataaactgg tcttccttgc ttctaaagga
      421 tgcagcttcc aagggattcc gctttgaccg tagacctgac ttatataaag attgaaagga
      481 gatatgtgat ggatagcatc agacaaagga taattgacgc acaaatggcg gcccagtctt
      541 tagtgattta ccgcagcata ttgaaagatc ccatagtgga gacatttatg caaatccttg
      601 acctggcctt gggtgaggct tcatttcagg aacttgttac aaaataccat gattttttca
      661 gccgacttgt caggcaaagc gaaaaccgtt cagaaccggc ttttgggaat ctgtggcaag
      721 accatatact aaatctggtc atcggagatg aaaacccctt cagtctccgc tgtgaaaaga
      781 cgggcattgc tggaataagc cgggatctca tcacactaac gcaaagggac ctcgcatcac
      841 ttcagaaaat acatgatttt gacttttcga tttttacatc atatattggg caaaaagccg
      901 gtgagcttgt tgtgggtttt cctgtgatgt acagcataga aagccaaaga catattccct
      961 accccgagag ctattttaag gagaaatatc atgtcaagaa attgatgggg aattctacag
     1021 actgggatcg aaatattgaa gttttagcaa agttttactt tgaaaagggc tgcggcatat
     1081 ttgcaaggtt ttgggccttc aagtgggtat gcaccgaaaa tggtggcgac ttggtgggaa
     1141 tatccgaacc ggatcccatc cgcttaaatg atctgatagg ctatgaagag caaaaacagc
     1201 aggtcttaag aaatacccgg caatttgttc aaggctttgg agccaacaat atgttgctgt
     1261 acggtgatag gggcacaggc aagtcatcta ccattaaagc cctggttcac gaatatggac
     1321 aagagggcct gagaatggtg gaagttacaa aagaccagtt gatgagcctt ccggatataa
     1381 ttgcgaaatt taataaccgt ccttatcgct ttataatttt tgtagacgat ctttcctttg
     1441 aagaacatga gacggaatac aagtatctaa aagcagtact tgaaggaagc cttgagcctg
     1501 ctcccgaaaa tgtcctcata tatgccacct ccaatcgccg ccaccttatc cgtgaatacc
     1561 acagcgaccg agtccaagat gaagtacggg cccaggacac cctccaggaa aagctttctc
     1621 tgtcagaccg tttcggaatt acagtcatat atccctctcc cgaccaagag gaatacttaa
     1681 agatagtcca gggcatagca ggaagcaggg gaatagatgt agagccggag cagctccggc
     1741 agatggcgct aaagtgggaa ctctggcaca atcgccgctc aggccgaact gccaaacaat
     1801 tcatagaaga cttgctaggc cgcctcagga aagcataaat aatacgcaat ctttgcagga
     1861 aaacggtgtg tgaccggctc tggaggaatg cccagccgtg acatggaagc ttttgaaatc
     1921 ggtctgcagg aggcaatgga ttctttcaag tgtgaggatg atggaaagag gaaatgtgtc
     1981 tgcaggaaga aataaagagg acaatgaata tatcgcaaga ctactaataa tataatagca
     2041 gtcttgcgat actttttttg cattttatta catcggcagt ttcgttactg gtttgatttt
     2101 atcctttctt gcttgtgtag gcactcatca caacatgtcc gagtattttt atttaactat
     2161 ttaaaggttt ttttcaagca atgtagaatt atttaaaagt agcaacagtc ctttttaagg
     2221 ggtgggtcta tggctcatac cgaccagata atagatctca tagatgaagg gataatagcc
     2281 gttgacagtc gcggctatat aactacttac aataagattg cccgggattt ttttggcatt
     2341 aatctaactg atgggcccgg ccatagggat ggaaaatgtg tagaaggcga ctgggttctc
     2401 atcgcggata atggtttggg tgaagatgac ggaggcatga aagcgcagga ctttaaagtc
     2461 ataggagttg atcccgtaga tatcatggaa ggggattcta tagttgctat cggcaggaaa
     2521 ggtggtcttt taggagaggg aatttataaa agactcgaga aacatacagc tcaaaaagag
     2581 ctttttgttg aaacatatat aaacggcata aaacttcggt cgatgattaa ttatgattta
     2641 aagcttttgc gcattactgt tggcgagcaa aattttgact acatttattt gtggtctgca
     2701 ggtcacatgg tcattgttga cggcaagaat atgcaggtaa agttttatca gagcaggggt
     2761 tacacggcgc gcaatgaaga tatgaagacc atactttatg gcggtcattt tatgggaaag
     2821 ggtatttacg gcaaatctat tgacgtagaa catatgcata taagtgaact gcatcctgac
     2881 tctgatattg taaaaaagct aacagatgta gccctcggtc aagattgcag cataaggggg
     2941 atggagactt ctataaacgg tattcccgtc cgctgttcga ttgaaccctt gaacaaagat
     3001 ggaaaaaggg ttggtgccct cctaaagttg actgacatta cggagataaa agccctttgg
     3061 catgaaaggg aaaaggcgct tttaactttg gagaccctgg aaaacaaact aaagacgttt
     3121 catataaagg aggaagcatt caaggatatt gttggcaaca gtgaaagtat caaatatgcg
     3181 gttgatctgg ctaaaagggc tgcagatacc agctctacgg tgcttttgtt gggtgaaagc
     3241 gggaccggga aaggagtttt tgccgaggcc attcataggg caagttctcg gcgggataaa
     3301 ccctttgttt acgtgaattg tgcttctatc cctgaagcct taatagagag tgagctattc
     3361 ggtcacgaaa gaggtgcttt tacaggggcg gtctccgaaa aaaaggggaa gtttgagatt
     3421 gcagacgggg gaaccatatt tctaga
//
LOCUS       DAONUO010000228         3417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19172_length_3417_cov_3.411660, whole genome shotgun sequence.
ACCESSION   DAONUO010000228 DAONUO010000000
VERSION     DAONUO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19172_length_3417_cov_3.411660"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK768_08165"
     CDS             <1..370
                     /locus_tag="PK768_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQA60986.1"
                     /translation="TNVKIDIEDDGKVYIAAPNEELGKKAMEIIDKLTQDVEVGGIYL
                     GKILRITNFGAFAEILPGKEGLIHISKLSKDRVKRVEDVVKVGDEILVKVTDIDKQGR
                     INLSHKDAIGDSKLKKHVQA"
     gene            462..1175
                     /locus_tag="PK768_08170"
     CDS             462..1175
                     /locus_tag="PK768_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA60987.1"
                     /translation="MTFFTIKISKRTVGFAVFIILIATWIFFRPSYRPIQVFGGNKPI
                     YQGSKDRKLMAFTCNVAWGNEYIPPLLEIFKNKNIKATFFVEGRWAEKYPELLKLIYE
                     CGHEIGNHGYSHAHHAELSYEQNLNEIKKAEQVIENIIGIKTTLFAPPYGEFSDLTVK
                     AASSMNYRLIMWTIDTIDWKKPGVDFIVNKVLENSGNGKIVLMHPTDDTVKAMPTIID
                     NLHREGFKITTVGQLLTAE"
     gene            1242..2447
                     /gene="alr"
                     /locus_tag="PK768_08175"
     CDS             1242..2447
                     /gene="alr"
                     /locus_tag="PK768_08175"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQA60988.1"
                     /translation="MINPTSWLEVDLDKISENVKNIKKYLGSTRLLAVVKANAYGHGL
                     YPVASCAVESGADFLGVSSIEEGIFLRKKGISQPLLVFNTILPEQAADVLDYGLTATV
                     CSLDVVQALNEVAARQNKKATIHVKIDTGFGRFGALPDHAAEMIRLIISNFDNIYLEG
                     IYTHFSKANNEKITYKQFNAFMSLVETLNEQDIEIPIKHVCNSVAALNYPEMHLDMVR
                     VGNLIYGMVPSKEIAITNPSKICSRVIFLKNLPKGHYIGYGNKYKTKKPTTIAVVPFG
                     YFDGLEVYVFQPNGFLDGLKYLIKQVLFRFGIYSSSRKANINGASCYILGKISMQNCI
                     VDVTKIKNNVFVGDVEELSARRLNLSQSIVRIYHRAGRVFSENDITPAETASDFAPPA
                     GQRRETSIG"
     gene            2440..2889
                     /gene="dut"
                     /locus_tag="PK768_08180"
     CDS             2440..2889
                     /gene="dut"
                     /locus_tag="PK768_08180"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQA60989.1"
                     /translation="MDRPIKIAVTRVKGAEDIPMPQYMTEGAAGMDLAANVAKNTYLE
                     PGEFKLIPCGIKIELPIGFEAQVRPRSGLAAKYGITVLNSPGTIDSDYRGEIKVILIN
                     HGKGKFEIKRGDRIAQLVISKVERVEIVQKHLSETSRGEGGFGHTGV"
     gene            2943..3230
                     /locus_tag="PK768_08185"
     CDS             2943..3230
                     /locus_tag="PK768_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HQA60990.1"
                     /translation="MRLSELGGKEIINLYNGERLGVLSNTDLVIDETTGEIICLVIPK
                     RRIPYLLFAERAKTEVSWNAIKKIGPDIVIIDMEDHGHSKYNIFIKGKNYT"
BASE COUNT         1191 a          528 c          724 g          974 t
ORIGIN      
        1 aaccaatgtc aagatagaca tagaggatga tggaaaagtt tatatagctg caccgaatga
       61 agaattaggt aaaaaggcta tggaaataat agataagctc actcaagatg tagaagttgg
      121 tggaatatat ctgggcaaaa tattaaggat tacaaacttt ggcgcctttg cagaaattct
      181 tccggggaaa gaaggcttaa ttcacatatc caagctttca aaagataggg taaaaagggt
      241 agaagatgtg gtaaaagtcg gagatgaaat ccttgttaaa gtaacggata tagataaaca
      301 aggcagaata aatttatccc ataaagatgc aattggcgat tcaaagctta aaaaacacgt
      361 acaggcataa accgatatac ggtttatgtt ttttatttgt cataaaatat ctctttcaaa
      421 ataatattta ttaaaaaacc atataaggaa gggatatttt tatgaccttt tttactatta
      481 aaatctctaa aaggactgtg ggctttgcag tctttataat tctcattgcg acctggattt
      541 tttttcgacc atcatatcgc cctattcagg ttttcggggg aaataagcct atttatcaag
      601 gcagcaaaga caggaagtta atggcattta catgcaatgt ggcatggggc aatgaataca
      661 taccgccatt attggagatt tttaaaaaca aaaatatcaa agctactttt tttgttgagg
      721 gcaggtgggc tgagaaatat cctgaattac taaaactaat ctatgaatgc gggcacgaaa
      781 taggcaacca cggatatagt catgctcatc atgcagaact ttcttatgaa caaaatttaa
      841 atgagataaa aaaagccgaa caggtaattg aaaatataat agggataaaa acgacactat
      901 ttgcaccacc ttacggtgaa ttctctgatc taaccgtcaa agccgcaagt tccatgaatt
      961 accgccttat tatgtggacc atagacacca tcgattggaa aaaaccaggc gtagatttca
     1021 ttgtgaataa agttttagag aattcaggaa atgggaaaat tgtacttatg caccctacag
     1081 atgatacggt aaaagctatg ccgacgatta ttgacaattt gcatcgtgaa ggctttaaga
     1141 taacaacggt tggtcaattg ctgacagcag aatgaaaaag ttgaaattaa gctatatttc
     1201 atgtatactt tatttaaaat taaagtagaa gggtaataaa tatgataaat cctacttctt
     1261 ggcttgaggt agacctggat aaaatatcgg agaatgtaaa aaatataaaa aaatatttag
     1321 gttcaaccag attacttgca gtagtcaaag caaatgctta tggacatggt ctatatcccg
     1381 tggccagctg tgccgttgaa agtggagcag attttttggg agtgtcttct atagaagaag
     1441 gtattttttt gcgaaaaaag gggataagcc aaccattatt agtttttaat acaattttgc
     1501 ccgaacaggc ggctgatgtc cttgactatg gcttaactgc tactgtttgt tctcttgatg
     1561 ttgtacaagc cttaaatgag gtcgctgcta gacaaaacaa aaaggccacc attcatgtaa
     1621 aaattgatac gggctttgga aggtttggag cgctaccgga tcatgctgcc gaaatgattc
     1681 ggcttattat atcaaacttt gataatatat atctagaggg tatttacaca catttttcta
     1741 aagcgaataa cgaaaagatt acttataaac agtttaatgc gtttatgtcc cttgtagaga
     1801 ctctaaatga acaggacatt gaaataccga ttaaacacgt ttgcaacagt gtagctgcgt
     1861 taaattatcc tgaaatgcac ttggatatgg ttagggtagg taatttgatt tatggtatgg
     1921 tcccgtcaaa agaaatagca attacaaatc cctcaaaaat atgttcaaga gtaatctttt
     1981 taaaaaatct tcctaagggt cattatatag gctatggaaa caaatataag accaagaagc
     2041 ccacaaccat agcggtagtc ccctttggct attttgacgg cctcgaagta tatgtatttc
     2101 aacctaatgg ctttttagat gggctaaagt acctcattaa acaggttttg tttagatttg
     2161 gaatctacag ttcaagcaga aaggccaata ttaacggagc gagttgctat atattaggca
     2221 aaatcagcat gcaaaattgt attgtagatg ttacaaaaat taaaaataac gtatttgtgg
     2281 gagatgtgga ggagcttagt gcaagacggt taaatctatc tcaaagcata gttagaattt
     2341 atcatcgggc tggtcgggtc ttttcagaaa atgatattac tcctgctgaa actgcttcag
     2401 attttgctcc tccagccggt caaaggagag aaacgtcaat tggatagacc gataaaaatc
     2461 gctgttacca gggtaaaggg agctgaagat atccccatgc ctcaatacat gactgaaggg
     2521 gctgctggaa tggatttagc agcaaatgta gcaaaaaata cctacctgga accaggggaa
     2581 tttaagctta taccgtgtgg catcaaaata gagctaccaa taggctttga agctcaggta
     2641 agacctagaa gtggtcttgc tgcaaaatac gggattacag tcctcaatag tccgggaact
     2701 attgattctg attatagggg agaaataaaa gtcatcttga tcaatcacgg caagggaaaa
     2761 tttgaaataa agagaggtga cagaatagcc cagcttgtca tatccaaagt cgaaagggtg
     2821 gaaattgtcc aaaagcactt gagcgaaaca tctagaggag aaggtggttt tggccatacg
     2881 ggagtttaac tttttcgcat tttttagcct cccaagtcat aaaatatacg ggaggtaaga
     2941 atatgcgatt gagtgaattg ggcggtaaag agatcataaa tctttataat ggagaaaggc
     3001 tcggggtttt aagtaatact gatttagtta ttgatgaaac aacaggagaa attatttgct
     3061 tggtaattcc taaaaggagg atcccttatt tactctttgc tgaaagagca aaaaccgaag
     3121 tatcttggaa tgctataaaa aaaataggac cggatattgt gattatcgat atggaagatc
     3181 atggtcacag taaatacaat atctttataa aaggcaaaaa ctatacttaa gttgtcggag
     3241 gtgttttttt attgccaaaa accggacaaa acaaattact catcattcct ttaggaggaa
     3301 tgggagaaat aggaaagaac atgaccgtta ttcaatatgg aaaagagatt tcgcttattg
     3361 atgcgggttt gatgtttcct gaggaagata tgctaggaat agacatggta atcccag
//
LOCUS       DAONUO010000229         3414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19191_length_3414_cov_4.210182, whole genome shotgun sequence.
ACCESSION   DAONUO010000229 DAONUO010000000
VERSION     DAONUO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19191_length_3414_cov_4.210182"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PK768_08190"
     CDS             <1..585
                     /locus_tag="PK768_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HQA60991.1"
                     /translation="RVMTERERRLVAYHEAGHAVVAHLLPSVDPVHEVSIIPRGRAGG
                     YTMILPKEDRFFMGKSELMDQVTHLLGGRVSEELVLNEISTGAQNDLERATQIARRMV
                     MEFGMSERIGPMTLGHKHEEVFLGRDLARGRNYSEEVAASIDKEVKNIIETCYNKAKT
                     LLSENINKLHKVAQALLEREKLDGEEFLEVFASA"
     gene            complement(638..919)
                     /locus_tag="PK768_08195"
     CDS             complement(638..919)
                     /locus_tag="PK768_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA60992.1"
                     /translation="MITATSKRRYDGKQALLSETVVVLKLGASGAGAACRKHAGRHSA
                     GCRIAREPLRSEDPSFSTTGLDYRDGAVISSLVNGLHCSPSFWLQQEQR"
     gene            1156..1614
                     /locus_tag="PK768_08200"
     CDS             1156..1614
                     /locus_tag="PK768_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQA60993.1"
                     /translation="MIGLSITKDMLFLLIAFLAGVAMAGQGTMNSALGKAIGLSESTF
                     VVHLTATLAMAAVLILGLGDGNWSNYDSIPWYYYFGGLIGVGITYGVVISIAKLGAAI
                     ATTSIIVGQVLTACLLDHFGIFGLEKSPLSWLKLLGVGLLALGAKLLLGK"
     gene            1701..2015
                     /locus_tag="PK768_08205"
     CDS             1701..2015
                     /locus_tag="PK768_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQA60994.1"
                     /translation="MALFKNALEFQAEFLRCLGDKTRLRIVKALMEREKTVSQLVQEL
                     QSSQANISGHLKTLKNSGILKSRQQGRYVYYSINDKDIEELITYLEHRILSFRRKAFE
                     GA"
     gene            2109..2627
                     /locus_tag="PK768_08210"
     CDS             2109..2627
                     /locus_tag="PK768_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQA60995.1"
                     /translation="MKMDPTVGRWQARTLTVSGLLGALSIMLGMTPLGFIPVPTAAGH
                     ATIMHIPVILGAVLEGPRVGALTGLIFGLHSFMRATSPLFADPLIAIVPRILIGLVAY
                     AVYRLSRSEALAAAFGTFTNTAGVLGLAVVRGYLPKAAAWTIAVTHGIPEIIIAVVIT
                     VVASGALRKVRR"
     gene            2632..3402
                     /locus_tag="PK768_08215"
     CDS             2632..3402
                     /locus_tag="PK768_08215"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQA60996.1"
                     /translation="MLLAVDIGNTNLVFGVFDGSKLIASFRIATHHDFTEDEYGLIFD
                     NLLRSKGINSKDFEGAIIASVVPPLTDVLEKMCDKYLALKPIIVGPGIKSGINIKYDN
                     PKEVGADRIVNAMAAYHKYGGPAIIVDFGTATTFDAITANCDYLGGAIAPGISISSEA
                     LFKSAARLYRVEIARPERIIGKNTAESIKSGIYYGYVGLVDNIVEKMKEEMGGGKIFT
                     VATGGLAPLICSEAKNIDAVDLMLTLEGLKLIYDKNKN"
BASE COUNT          954 a          725 c          843 g          892 t
ORIGIN      
        1 agagtcatga ccgaaaggga acgccgctta gtggcatatc acgaagccgg gcatgctgta
       61 gtagctcacc tgttaccttc ggtggatccc gttcacgaag tttctatcat accccgaggc
      121 agagccggcg gatataccat gattctgccg aaagaagaca gattcttcat gggtaaaagc
      181 gagctaatgg atcaggtaac tcatcttttg ggtggcaggg tatcagagga acttgtgcta
      241 aatgaaatta gcaccggtgc ccaaaacgac ttggaaaggg caactcagat agctcgaagg
      301 atggtaatgg aatttggcat gagtgaaagg atcgggccca tgacccttgg ccacaaacat
      361 gaagaggttt tcctgggaag ggatttggct agaggcagaa attacagtga agaagttgca
      421 gcttccattg ataaggaagt gaaaaacatc attgaaacct gctacaacaa ggccaagacg
      481 cttctgtcgg aaaacatcaa taagctccac aaagtagccc aggcattgct tgagcgggaa
      541 aaacttgacg gcgaggaatt cctcgaagtc tttgcaagtg catagattaa ataaatcttg
      601 gaggttgcga ttattgcaac ctcttttaat tttcgcctta tcgttgttct tgttgaagcc
      661 aaaatgacgg cgagcaatgg agtccattta caagcgaaga tatgacggca ccatccctat
      721 aatcaagacc tgttgtacta aagctcgggt cttcactccg caggggttcg cgggcaattc
      781 ggcatcctgc cgagtgcctg ccagcgtgct tcctgcacgc tgcccctgct ccgcttgccc
      841 cgagcttaag aacaactacg gtctcgctta aaagggcttg cttgccgtca tatcttcgct
      901 tagaagtcgc ggtaatcatc tatagaagat gcttattgtc actgcctcgc agttacacct
      961 tcactacaaa gtggagaaaa tcatctataa agtaatcact tgctgccttg ctcaccgtca
     1021 tttttatctg gaaatagcaa caattctcta ttaaagtcgg tcattaattc ttcatccctt
     1081 ctatacatta tctataaagc tagattaatt tacgcgagta taaattccaa ctcagcgagg
     1141 tagtctaaat taagggtgat tgggttgtca ataactaaag acatgttatt tctattgatc
     1201 gcttttttag ccggtgtcgc catggcgggc cagggcacga tgaattctgc cctcggcaag
     1261 gccatcggcc tttcggagtc aacatttgta gtacacctca cagccacatt ggcaatggct
     1321 gccgtactaa tattgggttt aggggatgga aattggtcaa actacgacag cattccctgg
     1381 tattattatt ttggaggtct catcggggta ggtattacct acggtgtagt gataagcatc
     1441 gctaaattgg gagcagccat agcaacgacc tccattattg tgggccaggt gctcaccgcc
     1501 tgtttgctag accacttcgg gattttcgga ttggaaaaat ctccgctttc ctggcttaag
     1561 cttttaggtg tcggattatt ggctttaggt gccaaactct tgttaggaaa atgacaagaa
     1621 agcatatatg ctgaaggcta aaaaaagttg acaaaacatg atataataaa atcggcagat
     1681 ctagtttagg aggttgtgcc gtggcattat ttaaaaatgc actggagttt caggcggagt
     1741 ttttgcgttg cttgggcgat aaaaccaggc tcaggattgt caaagccttg atggagcgcg
     1801 aaaaaaccgt atcccaatta gtccaggagt tgcaaagttc acaggcgaat atttccggcc
     1861 acttaaagac cctaaagaat agtggaattt taaagagcag acagcaaggc aggtacgttt
     1921 attacagcat aaacgacaaa gatatagaag agctgataac atacttggaa cacaggattt
     1981 tgagctttag gcgaaaagct tttgaaggag cataaattat cattattgat tttcaaataa
     2041 tcccttggta aaattaaaaa gaaatacgtc cggcatcctt acaaggagct ggaacggagg
     2101 tggaaagaat gaagatggat ccaacggttg gacgatggca agccaggacc ttgaccgtgt
     2161 cgggactttt aggagctctg tccataatgc tcggcatgac gcctctgggc ttcataccgg
     2221 ttcctactgc ggctggccat gccaccatta tgcacattcc tgtaattctc ggtgccgtat
     2281 tggaaggacc aagagtagga gccctaacgg gtttgatttt tggccttcac agcttcatga
     2341 gggcaactag ccccttgttt gcagacccac ttattgcaat agtaccgagg attctcatcg
     2401 gacttgtggc atatgctgtc taccgtctaa gccgtagcga agctttggca gcagcttttg
     2461 gcacctttac caatacggca ggtgtcttgg ggctggcagt tgtgcgggga tatctgccga
     2521 aggcggcagc ctggacaatt gctgtgacac acggcatacc tgaaatcatc atagctgttg
     2581 tcattacagt tgtagcttcc ggggcattgc ggaaagtgag aaggtgaaaa agtgttactg
     2641 gcagttgata taggtaatac gaacctggtc tttggagttt ttgatggctc aaaacttata
     2701 gcatcattta gaatagctac ccatcacgat tttacggagg acgaatacgg cctcattttc
     2761 gacaacctgc tcaggtcaaa gggcataaac tcaaaggact ttgaaggtgc aataattgcc
     2821 tccgttgttc ctcccttgac ggatgtttta gaaaaaatgt gcgataaata ccttgcacta
     2881 aagcccatca tagttggtcc cggcattaaa agcggcatca acataaaata cgacaatccc
     2941 aaggaggtag gagcggaccg catcgtaaat gctatggcag cttatcacaa gtatggcggc
     3001 cctgccataa tagttgactt tggcacggca accacctttg atgccattac agcaaattgt
     3061 gattacctgg gcggtgccat tgcccccggc atcagcattt cttcagaagc tctttttaaa
     3121 agtgcagcta ggctttatcg ggtggagatt gcccggcccg agcggataat cggaaagaac
     3181 acggctgaaa gtattaagtc aggtatttat tacggatacg taggccttgt ggacaatatt
     3241 gtagagaaga tgaaggaaga gatgggtggc ggcaagattt tcaccgtggc cacgggaggt
     3301 cttgcccccc ttatatgcag cgaagccaaa aatatcgatg ctgtcgattt gatgctgacc
     3361 cttgaagggt taaagcttat atatgacaag aataagaact agtatttaag ggaa
//
LOCUS       DAONUO010000230         3405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19264_length_3405_cov_4.073731, whole genome shotgun sequence.
ACCESSION   DAONUO010000230 DAONUO010000000
VERSION     DAONUO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19264_length_3405_cov_4.073731"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PK768_08220"
     CDS             complement(<1..584)
                     /locus_tag="PK768_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA60997.1"
                     /translation="MALIDIQNLNFAYPNSPQRALKNINFSVEAGEFVVICGPSGCGK
                     TTLISQLKREIRPEGEISGRILYRGKPIDCLSKKEAVEEIAMVFQDPENQIVLDTVIH
                     ELAFGMENLGYDILTMKKRIGEMCNFFGLEKMLYKSVHELSGGEKQILNLASVLLLQP
                     KLLLLDEPTSQLDPIAAKEFLQMLYRLNRELSMTV"
     gene            complement(575..1447)
                     /locus_tag="PK768_08225"
     CDS             complement(575..1447)
                     /locus_tag="PK768_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQA60998.1"
                     /translation="MNRGFASLHPAPCFLYYVGLVILVMIIYHPLFLLLALCSQILLN
                     IMQDGGRKLKESARFYIFFGLFIALINPFVSRRGQNVLFYFMDNPVTLEAVVYGIAMM
                     MSLLTVLIAFVSYNMVITPDKFMFLFSSILSKTAFMAMMAMRFVPVLKRRSQEIALVQ
                     KTRGTDISSGTFIQRIKNGMQILVILITWSLEEAIITSRSMRARGYGVTKNRSSYFDY
                     KMTRRDWTILFLIVLSWVNLLFLWYQGIGYFQIYPELGQLAFDMNTGLLLVASSVYLA
                     IPLSVEGAEKLAWL"
     gene            complement(1440..2126)
                     /locus_tag="PK768_08230"
     CDS             complement(1440..2126)
                     /locus_tag="PK768_08230"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HQA60999.1"
                     /translation="MRLFMDRQTLFLVLILLIACLLIVGCGSKASAPETSPRSEGRQE
                     NIQQEADISHGQEGQKQEESIDRVDPAPKAEESKTSPEKSSSNGKGQEDEKSQSKEEE
                     KPGDADPAPSVTISIVGHKAMGVILEATEVEIEEGETVLDALKKAAKQNNIPISIRGK
                     KSTAYVEGINNLFEFDHGPRSGWLYRVNGSMYSKSAGAFTVNAGDVIEWLYTLDLGRD
                     IEDSGGAADE"
     gene            complement(2123..>3405)
                     /locus_tag="PK768_08235"
     CDS             complement(2123..>3405)
                     /locus_tag="PK768_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA61000.1"
                     /translation="EYLEEYLKEHEGDFRKITDYAKLAMAVSALNRDPRNVAGYNIIE
                     KIYNNGNMTLQGTNGPIFALIALDACGILIPDDALWTRQKLLDYILNQQNPDGGFSLV
                     YGEISDIDLTAMAIQAMSLYRDKPEVAAAIERAIEFLSQKQSADGGFTAWGDSSSESI
                     SQTIIALIALEIDPLKDPRFIKDGNLISKLLSFREADGGYSHIEGEGSDEMATEQALV
                     ALVAYKRFLDDESWIYHLTEGKESSPEDHTSFADIDSASEWARQSIEKAREYGLMEGK
                     GRNLFEPKQNITRAEFAALLVRLLKLEGKSSAQQVFSDVKPDSWYFGYVMNCYEKGII
                     TGKSQDKFMPNDYITREEMAVMLQRALSLETAEEVDLKDLQDASQWAVPAIKAIVGNQ
                     LMTGANGNFSPRQNVTREMAATVMVRVFERGEGQ"
BASE COUNT          846 a          842 c          716 g         1001 t
ORIGIN      
        1 accgtcatgg aaagttccct gttgagcctg tacagcatct gtaaaaactc ttttgccgcg
       61 atcggatcaa gctgggaagt gggctcatct aagagcagca gcttcggttg aagcagcagt
      121 accgaggcca agttcaatat ctgcttctcg ccgccagaga gttcgtgtac ggatttataa
      181 agcatttttt ccagtccgaa gaaattgcac atttccccta tccgcttttt catggtaagg
      241 atgtcgtagc ccaggttttc catgccaaaa gccagttcgt gtatgacagt atcaaggact
      301 atttgatttt ctgggtcctg aaagaccata gcgatttctt ccacggcttc tttttttgaa
      361 aggcagtcaa ttggttttcc ccggtacaaa atcctgccgc taatctctcc ttcaggccgg
      421 atttctcttt tcaactggct tatcagggtt gtctttccgc agcctgaagg gccgcatatg
      481 accacaaatt caccagcttc tacagagaaa tttatatttt ttaaagccct ttgaggggaa
      541 tttggataag caaagtttaa attttgtatg tctattaaag ccatgccagt ttttcagctc
      601 cttctacact taagggaata gcaaggtaca cagaagatgc tactaataat agtcccgtgt
      661 tcatatcaaa agcaagttgc ccaagctccg gatatatctg aaagtatccg atgccctgat
      721 accataaaaa cagtagattt acccaggata aaactatcag aaaaagaatt gtccaatccc
      781 ttctagtcat cttatagtcg aaatatgagc tcctgttttt tgtaacaccg tatcccctcg
      841 ccctcatgga cctcgatgtg ataatggctt cttccaaaga ccatgtaatt agtattacca
      901 gtatctgcat gccgtttttt atgcgctgta tgaaagtccc ggaagatata tccgtgcccc
      961 tggtcttttg gacaagggca atttcttggc tgcgcctttt gaggaccggc acaaagcgca
     1021 tggccatcat ggccataaag gcagtcttgg agagtattga agaaaaaaga aacataaatt
     1081 tgtccggagt tatgaccatg ttgtaagaaa caaaagctat tagcacggtc agaagggaca
     1141 tcatcatggc aatgccgtaa accacagctt caagggttac gggattgtcc atgaaataaa
     1201 agagcacatt ctgaccccgg cgggaaacaa agggatttat caaggctata aacaatccaa
     1261 aaaatatgta gaatctggcg gattccttta gcttcctgcc cccatcctgc atgatattga
     1321 gtagtatttg agagcagagg gccaagagta aaaacagagg gtggtagatt atcatcacaa
     1381 gtatgacgag accgacataa taaagaaaac agggggcagg atgaagcgat gcaaagcccc
     1441 tattcatctg ccgcccctcc tgaatcctct atatctcttc ccaaatcaag ggtatagagc
     1501 cactcaatca catcaccggc attaacagta aaagctccgg cacttttgct gtacatgctg
     1561 ccgtttaccc tgtaaagcca gccgctcctt ggcccatggt cgaattcaaa caagttattt
     1621 atgccttcca catatgccgt ggattttttc cctctgatgg atatgggaat attgttttgc
     1681 tttgctgcct ttttcagcgc atccagcacc gtttcccctt cttctatttc aacctccgtt
     1741 gcttccaaaa tcacgcccat ggccttgtgg ccaactatgg atatggtaac ggaaggggcc
     1801 ggatccgcat ccccgggctt ttcttcttcc ttgctttgac tcttttcatc ttcctgacct
     1861 tttccgtttg aactactttt ttctggactt gttttagatt cctccgcttt cggtgcagga
     1921 tctaccctgt caatactctc ttcctgcttt tgcccttcct gaccgtggct gatgtctgct
     1981 tcttgttgaa tattctcctg gcgcccttca gatcgaggag aggtctcggg agcggacgcc
     2041 ttgctgccgc agcccactat taataaacag gctatcagca gtattaaaac taaaaacagg
     2101 gtctgtctgt ccatgaatag tctcactgcc cttcgcctct ttcaaatact cttaccatta
     2161 ccgtggctgc catctcccgg gtaacatttt gtcttggcga aaagttgccg tttgcccctg
     2221 tcatcaactg atttcccact atagccttaa ttgccggaac cgcccattgg gacgcatcct
     2281 gtaaatcttt aaggtcaact tcttctgctg tctctaaaga caaagcccgc tgcagcatca
     2341 cggccatttc ttcccttgta atataatcat ttggcatgaa tttatcttgg ctttttccgg
     2401 tgattatccc cttttcgtag cagttcatga cataaccgaa gtaccagctg tcgggtttga
     2461 catcactgaa cacctgctgt gcactgcttt ttccctctag cttaagtagt ctaacaagta
     2521 gtgcagcaaa ttctgcacga gtgatattct gctttggttc aaagaggttt cgccctttgc
     2581 cctccatgag gccgtattct ctagcctttt cgatggattg cctggcccac tcggaggcgg
     2641 aatcaatatc tgcaaaggaa gtatggtctt caggagaact ttcttttccc tctgtcaagt
     2701 ggtaaatcca gctttcgtca tctaggaagc gcttataagc caccaaagcc actagcgcct
     2761 gttcggttgc catttcatcg ctgccttcac cttcaatgtg ggagtaaccg ccgtctgcct
     2821 ccctaaagga taagagcttt gatatgaggt tgccgtcttt tataaatctt gggtctttta
     2881 aggggtcaat ttccagggcg atcagggcta ttatggtctg ggaaatgctt tcactgctgg
     2941 aatcgcccca ggcggtaaag ccaccatcag cagactgctt ttgtgacaaa aactcgatgg
     3001 ccctttcaat ggcagccgca acttccggct tgtcccggta cagggacatt gcctgaatgg
     3061 ccatggcagt aaggtctatg tcacttattt ccccataaac cagggaaaag cctccgtcag
     3121 ggttttgctg gtttaaaatg taatctaata gcttttgcct ggtccacagg gcatcatcgg
     3181 gaatcaaaat gccgcaggca tccaaggcta tcaatgcaaa gatggggcca tttgtgccct
     3241 gcagggtcat attaccattg ttataaatct tttctataat gttatatccg gcaacatttc
     3301 tcgggtcacg gtttaacgcc gaaacagcca ttgccagctt tgcataatcg gtgattttcc
     3361 taaaatcgcc ttcgtgttct tttaggtatt cctccaggta ttcat
//
LOCUS       DAONUO010000231         3396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19329_length_3396_cov_3.143071, whole genome shotgun sequence.
ACCESSION   DAONUO010000231 DAONUO010000000
VERSION     DAONUO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19329_length_3396_cov_3.143071"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK768_08240"
     CDS             <1..741
                     /locus_tag="PK768_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HQA61001.1"
                     /translation="IVFIAVTALFAAYAVYQGLETICTLGEIIAPVILASVVLVFVMI
                     AKDLDLSNLQPVLEDGLAPVLYGAFVIAARTTLGLFLWILIPFINESGKIRNSLLIIF
                     LIFALHLSPSAIVTIGVFGLEQAKSLDFPFFHVVRMISIGDFLERIDALFVGFWVIGM
                     FIDISTHYYLAVLTGADLLKLKDYRPIVLAMGTVMVSLSMFLADSMVDLNEFLSYKTL
                     TWYNLLFTFAIPLLLLLVAVVGKKRVDY"
     gene            742..975
                     /locus_tag="PK768_08245"
     CDS             742..975
                     /locus_tag="PK768_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61002.1"
                     /translation="MKKLGLIFLAITIMAAPLIGCWSRRELDTLSIISAIGIDKSEED
                     GKLSVTFQIIKPSALKGSSSSEAALQVPQVFWF"
     gene            966..1916
                     /locus_tag="PK768_08250"
     CDS             966..1916
                     /locus_tag="PK768_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HQA61003.1"
                     /translation="MVLTSTGYTIIDAARNATMQSDRKLFFPQNKVIVVGEELAREGI
                     NPLLDFLNRDPEQRRSSWLLVAKGKAHDIINAKHEQVNIPAEAIVNMIKSSSNTSTAV
                     KVDINEFIKNLTNPGSDPIACRIEMIQDDKTKNQKMRFTGTSVFKRDKLIGFLDQYET
                     RGLNWVLGKVVSGIILVKSPLDENKDVALEIIRARSKITPEILNNDIKTIVEVDAEGN
                     LAEQLSEVTLTNSEMFKKLEQRMAQVIKDEIQLVLEKAQKEWGVDIFGFGKAVHRKYP
                     QVWKKLQSNWRELLSEVNVEIKVKAKIRSSGLSTIPTGVR"
     gene            1932..2147
                     /locus_tag="PK768_08255"
     CDS             1932..2147
                     /locus_tag="PK768_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61004.1"
                     /translation="MIVLLISVFVIIALFQMPALIKLKMWRELTAISVLLGIGFVLSL
                     LQLIGVKIPSPNQWIIFLIKSVTNFFG"
     gene            complement(2194..3306)
                     /locus_tag="PK768_08260"
     CDS             complement(2194..3306)
                     /locus_tag="PK768_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61005.1"
                     /translation="MANTDNVSKSTGKQVSWQIKKAVTGNIVPIVFTLLCLVGAVIAE
                     ASPIFLLSSVIIRLARNLLLVFSLIIPVVTGLGLNFGIVLGAMAAQIALVAVTHWDIG
                     GLGGFALAALLSVPLSIVFGWLTGKVLNKAKGNEMITSMMLGFFANGLYQLLFLFVLG
                     GIIPMVNETIMLPSGIGVKNTVSLKGIKYALDDVLKIGYETLTIPVFTFIVIALFAFV
                     IKFLLQTKLGQELRCVGQDMNIAATSGIDVDRMRIIAVILSTVLAAWGQLIHLQNIGI
                     LQTYGSHEQVGLFAVASILIGGASVTKATIGQAVLGTFLFHLLFIISPMAGQNLVGEP
                     QVGEYFRAFVAYGVIGVSLGLHAWQKQQAKKHMLEE"
BASE COUNT          992 a          729 c          730 g          945 t
ORIGIN      
        1 atagttttca tagctgtcac agccttattt gccgcttatg cagtttacca aggccttgaa
       61 accatttgca cgctcggaga aatcatagct cctgtaatcc tcgcttctgt agttttagtt
      121 tttgtcatga tagcaaagga tttggattta tccaatctcc aaccggtgct ggaagatgga
      181 ctggctcccg tcctctacgg tgcctttgtt atagcggcaa ggacgacttt agggttattc
      241 ctgtggatac tcataccctt tataaatgaa tcggggaaaa ttagaaattc tctgctcata
      301 atctttttga tttttgctct tcatctgagc ccttccgcca ttgttacaat aggggtcttt
      361 gggctggagc aggcgaaaag tttagatttt ccttttttcc acgttgtccg gatgataagc
      421 ataggggatt ttttggaaag gatcgatgcc ctctttgtag gcttttgggt gataggcatg
      481 ttcatcgata tatcgacaca ttactattta gcagtattaa ccggagctga tctgctaaaa
      541 ttaaaagact atcggccgat agtgctggcc atgggaaccg taatggtcag cttgtcaatg
      601 tttctggctg acagcatggt tgacctaaat gaattcttat catataaaac attaacatgg
      661 tacaacttgc tttttacttt tgccattcct ctgttactgc tgctagtagc cgtagtcggc
      721 aagaaaaggg ttgattatta agtgaaaaag cttggtttaa tcttcttagc cataaccatt
      781 atggctgctc ctctaattgg ctgttggagc cgccgggaac tcgatacctt atctataatt
      841 tcagctattg gtattgataa atcagaagaa gacggaaagc tcagcgtcac ttttcaaatc
      901 ataaagccca gtgcactaaa gggctccagc tcctcggaag cagcccttca ggttccacag
      961 gtgttttggt tttaacctcc acgggttata cgataattga tgccgccaga aatgccacca
     1021 tgcagtcgga caggaaacta tttttccctc aaaacaaagt catagttgta ggagaggaat
     1081 tggcccgaga aggtataaac cccctactgg actttttaaa tcgtgaccca gagcagaggc
     1141 gcagcagctg gctgctggtg gccaagggca aagcccacga tattatcaac gccaagcacg
     1201 aacaggtaaa catcccggct gaggccattg tcaatatgat caaatcaagt tctaatactt
     1261 ccacggctgt aaaggtagac ataaatgagt ttataaagaa tctaacgaat cccggttctg
     1321 accctatcgc atgccgcatt gaaatgattc aggatgacaa gacgaaaaat caaaagatga
     1381 gattcaccgg cacttcggtg ttcaaaagag ataaattaat tggtttttta gaccagtatg
     1441 aaacacgtgg tctgaactgg gttttgggta aagtagtgag cggaattatc ctggttaaat
     1501 ctcccctaga tgaaaataaa gatgtagcct tagaaatcat aagggcaagg agtaagataa
     1561 ctcccgaaat cttaaataat gatattaaaa caatcgtcga ggtagatgca gaaggcaact
     1621 tggccgagca actttcggaa gttacgctta caaattcaga aatgtttaaa aagctcgagc
     1681 agcgcatggc tcaagtcata aaggatgaaa tccaattggt tttagaaaag gcacaaaagg
     1741 aatggggcgt tgacattttc ggatttggca aggctgttca cagaaaatac ccccaggtgt
     1801 ggaaaaagct tcaaagcaac tggcgggaac tgctctcaga agtgaatgtg gagatcaaag
     1861 tcaaggcaaa gattcggtcc agcggccttt caactattcc tacgggggtc agataaagcg
     1921 gggtgtaatg tatgatagta ctgctgatat cagtttttgt aataatagca ctgtttcaaa
     1981 tgcccgcttt aattaagctc aaaatgtggc gggaacttac ggccatttcg gtgctgcttg
     2041 gcattggttt tgtacttagc cttttgcagc taattggagt caaaatcccg agtcctaatc
     2101 aatggataat cttcttaatc aaaagcgtaa ctaatttttt tggataagaa aaatgaggtg
     2161 gctgccacct cacttttgta gtaaatacag ttattattct tccagcatat gcttttttgc
     2221 ctgttgtttt tgccaggcat gaaggccgag agaaacacct atgaccccat aggcaacaaa
     2281 ggccctgaag tattcgccca cctggggctc tccaactaag ttttggcctg ccatcggtga
     2341 tatgatgaag agtagatgga agaggaaagt cccgaggaca gcctggccaa tagtcgcctt
     2401 tgtaacagat gcaccaccta ttaatatgga agcaaccgcg aaaaggccaa cctgttcgtg
     2461 actcccgtaa gtttgcaaga ttccgatgtt ctgcaaatgt attagctggc cccaggccgc
     2521 tagcaccgtt gatagaataa cagcgataat cctcatcctg tccacatcta tccctgatgt
     2581 ggcggctata ttcatatctt gacctacgca tcttaactcc tggcccagtt ttgtctgtaa
     2641 taaaaacttt atgacaaatg caaagagcgc aattactatg aaggtaaaaa ccggaatagt
     2701 caaagtctcg taaccaatct ttaagacatc atctagggca tactttattc cttttaggga
     2761 aacggtattt ttcacgccta tgcccgaggg taacattatg gtttcattga ccatagggat
     2821 tatgccaccc aggacaaata aaaacaaaag ctggtagaga ccgtttgcaa aaaagcccag
     2881 catcatactg gtgatcattt cattgccctt agctttattt aatacctttc cggtaagcca
     2941 tccaaatact attgacaaag gaactgataa gagggctgca agggcaaagc cacctaggcc
     3001 gccaatatcc cagtgggtca ctgccaccaa ggcgatttgt gcagccattg cacccaaaac
     3061 tatcccaaag tttaggccta agccggtaac aacaggtatt atcaatgaaa acacgagaag
     3121 caaattgcgt gcaagcctga tgattactga acttaacaaa aagatgggac tggcttcagc
     3181 tataactgct cccaccaaac acaacagtgt aaaaacgatc ggaactatat tacccgtcac
     3241 ggccttttta atttgccacg atacttgctt tccggttgat tttgagacat tatctgtatt
     3301 tgccaagctc attcacctcc gcctatcctg gttagagcat atacaatgat tccgttactt
     3361 attataatcc tcactacttc tgatagattt ccctcc
//
LOCUS       DAONUO010000232         3387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19387_length_3387_cov_4.222989, whole genome shotgun sequence.
ACCESSION   DAONUO010000232 DAONUO010000000
VERSION     DAONUO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19387_length_3387_cov_4.222989"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="PK768_08265"
     CDS             <1..1657
                     /locus_tag="PK768_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HQA61006.1"
                     /translation="IHNRREHKLIVSADSINCRIHLSDTVNQNPSSPPMFCMLLRKHL
                     LGGKIISILQKGLERIVEIAITNTDEFMQPKDYKLIVEIMGKHSNIILTDFQNTIIDS
                     VKRIGLELNRYREILPGKTYSDPPLDEKINLLSVDDDCIISMLKEASASQFTTTLSRW
                     IIDNFAGFSGVSAQELALRARLDHRIPICQLNNRQIEQIAKVFSNLREDLLQCHFLPR
                     VYCNINTKEPVDFWLFPMEKYNQKEYKSSNSVNEAADFFYSKKEEFVAVKTLRQSLKA
                     EVLKHEKKLRQKLQYLKERIDNTSDLNKYKLWGELLSANLYRIKAGQSQVKLPNFYNL
                     NEEVTIPLNEKLSPSHNAQLYFKMYKKLQATKKYVEARIKDTLMEMEYLESTLVNIEH
                     SRSIQDLTEIQQELELQKYIKTNVPKGKMESKKELSKPLRFKSSDGNVIYVGKNNRQN
                     DMLTFKKSKPDDIWLHAKNTPGSHVIVETQGHAASDTTLTEAAILAAYFSKGRNSSNV
                     PVDYTLVRHVKKPSGAKPGFVIYFNQKTLYVTPDEKTVDKLSF"
     gene            complement(1646..2182)
                     /gene="pyrR"
                     /locus_tag="PK768_08270"
     CDS             complement(1646..2182)
                     /gene="pyrR"
                     /locus_tag="PK768_08270"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQA61007.1"
                     /translation="MEEKARIMDENAINRALKRISHEIIEKNKGVDNLAIIGIRRRGV
                     PLAKRLADYIYSIEGEEIPVGILDITLYRDDLSSLAPSPVVHKTEIPFNITDMNLVLV
                     DDVIYTGRTVRAALDALADLGRAKKIQLAVLVDRGHRELPIRPDFVGKNVPTSREEIV
                     EVRLDEVDGENCVLILER"
     gene            complement(2184..3110)
                     /locus_tag="PK768_08275"
     CDS             complement(2184..3110)
                     /locus_tag="PK768_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQA61008.1"
                     /translation="MDKRHEFVIAKDNSKKRLDIFLAENLQESRSFLQKGIIEGWVKV
                     NGVCVKPNYKLKVGDHITVFIPPPPQPSITAENIPLDIIYEDKDILVVNKPRGMVVYP
                     APGNYSGTLINAVLFHTKDLSVRSGLMRPGIVHRLDKDTSGIIVLAKTDCAHKSISRQ
                     LKNREVTKLYLALVWGIVQEEKGTINAPIGRHLVKRTKMQVTAKNSRDAITHFKVIER
                     FEDITLLELSIETGRTHQIRVHMQYIGHPIVADPVYSRKKPPFDIEGQALHAYKLGFR
                     HPSNDKYVEFTAPMPDDMKAILEILRKEKMGE"
     gene            complement(3127..>3387)
                     /locus_tag="PK768_08280"
     CDS             complement(3127..>3387)
                     /locus_tag="PK768_08280"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQA61009.1"
                     /translation="SIVIVIITLVLIKLPAKHKAFGIVMALILGGAMGNFIDRLRFGY
                     VVDFLDFRVWPVFNIADCAIVIGVLILAYLITFHPEYQSLFK"
BASE COUNT         1066 a          727 c          614 g          980 t
ORIGIN      
        1 cattcataat agaagggaac acaagctgat tgtatcggca gattctataa actgcagaat
       61 ccatctttcc gatactgtaa atcagaatcc ctcctcacct ccaatgttct gcatgttgct
      121 tagaaagcat ctcttgggag gaaaaattat atcaatcctc cagaaaggct tagagcgcat
      181 agtagagatt gccatcacca atactgatga attcatgcag ccaaaggact ataaacttat
      241 agtggaaatt atgggaaagc acagcaatat aatactgact gacttccaaa atacaatcat
      301 cgattcagta aagcggatag gtctagagtt aaatagatac agggaaatac tgcctggaaa
      361 aacttattcc gatccgcctt tagatgagaa gataaattta ttgtcggtag atgatgattg
      421 cattatttcc atgttgaaag aagcatccgc atcgcaattt acaacaacac tgagcaggtg
      481 gattatagac aatttcgcag gcttttcggg agtgtctgcc caggaacttg ccttgagggc
      541 caggctagac catagaattc caatatgcca gctcaataat agacagatcg agcaaatcgc
      601 caaggttttt tcaaatttac gggaagatct gttacaatgc catttcctac ctcgtgttta
      661 ttgtaacatt aatacaaagg aacctgtaga tttttggctc tttcctatgg agaaatataa
      721 tcaaaaagaa tataagtcat caaactctgt aaatgaagct gcagactttt tctactcaaa
      781 aaaggaggaa tttgtggcag ttaaaacttt gagacagagt ttaaaagctg aagtcttgaa
      841 acacgaaaaa aagctgagac aaaagctcca atatctaaag gaaagaatag acaacacatc
      901 tgaccttaac aaatacaaat tgtggggaga attgttgtct gccaacttat atcgaataaa
      961 agccggccaa agtcaggtaa agctcccaaa cttctataat ttaaatgaag aggtaacaat
     1021 tccattaaat gaaaagctat caccttcaca caatgcccaa ctctatttta aaatgtataa
     1081 aaaattacag gccaccaaaa aatatgttga ggcaagaata aaggatactt taatggaaat
     1141 ggagtatctt gaaagcaccc ttgtaaacat agaacatagc cgatccatac aagatttgac
     1201 ggaaattcag caggaattgg aattacaaaa gtacatcaaa acaaatgtgc ctaagggtaa
     1261 aatggaaagt aaaaaagaat tatcaaaacc cttacggttt aagtcttcag acggcaacgt
     1321 aatttatgta gggaaaaaca accggcaaaa tgacatgctt acctttaaaa aatcaaagcc
     1381 tgatgatata tggctccacg ccaaaaatac cccaggctcc catgtcatag tagaaacaca
     1441 aggacatgca gcaagtgaca cgacccttac tgaagctgcc attttagccg catattttag
     1501 caaaggtcga aacagcagta atgttccggt agattacacc cttgtccgcc atgtcaaaaa
     1561 gccgtcaggc gccaaaccgg gttttgtcat atattttaat caaaaaactt tatatgtaac
     1621 tccggatgaa aaaactgttg acaaattatc tttctagtat cagcacacag ttttccccgt
     1681 ccacctcatc caatcttacc tctactatct cttcccggga ggtggggacg ttttttccaa
     1741 caaaatccgg tcttatgggc aattccctat ggcccctgtc tacgagtact gccaattgta
     1801 ttttctttgc ccgtcccaaa tcggccaaag catcgagggc agctcttacc gtcctgcccg
     1861 tatatataac atcatccact aaaaccagat tcatatcggt tatattaaag ggtatttctg
     1921 tcttatgcac caccggtgat ggggcgaggc ttgacaaatc gtcacgataa agagttatat
     1981 ccaaaatacc caccggaatc tcttcgccct caatggaata aatgtagtca gctaatctct
     2041 tggcgagggg gacacccctt cgccttatac ctatgatcgc caggttgtca acgcctttgt
     2101 ttttctcgat gatttcatgg cttatcctct ttaaagcacg attgattgca ttctcatcca
     2161 ttatccgcgc tttttcttcc attttactcc cccatttttt cttttcttaa tatttctaat
     2221 atagctttca tatcatcagg catgggcgcc gtaaactcta catacttgtc gttgctggga
     2281 tgacgaaaac caagtttgta agcgtgaagg gcctgccctt ctatatcaaa gggcggtttt
     2341 tttctggagt agaccgggtc ggcaacaatt ggatggccta tgtattgcat atgaactcgt
     2401 atttgatggg ttctccccgt ctcaatgcta agctcaagca gtgtaatatc ctcaaatctc
     2461 tctattactt taaagtgggt tattgcatcc cttgaattct tagcagtaac ctgcatttta
     2521 gttcgcttta ccaggtgtcg tccaatggga gcatttatgg tacccttttc ttcctggact
     2581 attccccaaa ctaaggccag atataacttg gtaacttcgc gatttttaag ttgccttgat
     2641 atgcttttgt gggcgcagtc agttttcgcc agcactataa ttccagaagt atccttatct
     2701 aggcggtgta caataccggg ccgcattaag ccgcttctga cagataagtc tttcgtgtgg
     2761 aaaagtacgg catttatgag cgtaccagaa tagttacccg gagcgggata tactaccatg
     2821 cccctaggct tatttacaac taaaatatcc ttatcttcat aaattatatc caaaggtatg
     2881 ttctcagcag tgatagaggg ctgcggtggc ggaggaataa acaccgtgat atggtcccct
     2941 acttttaatt tataatttgg cttcacacaa actccattaa cttttaccca gccttcaatt
     3001 attccttttt gtaaaaaact acgggattcc tgcaaatttt cagctaaaaa aatatccaat
     3061 cgcttttttg aattatcttt tgcgattaca aactcgtgtc ttttatccaa gttcttcctc
     3121 ctgtttttac ttgaacaggc tttgatattc cggatgaaat gtaattagat atgctaatat
     3181 cagaacccct atgactattg cacagtcagc tatgttaaag accggccaaa ctctaaagtc
     3241 taaaaaatca actacatatc caaatctcag tctatcaata aaattgccca ttgcccctcc
     3301 tagaatcaaa gccattacaa taccaaaagc cttgtgcttt gcggggagct taattaatac
     3361 taaagtgatt ataactatta ctataga
//
LOCUS       DAONUO010000233         3373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19491_length_3373_cov_3.657022, whole genome shotgun sequence.
ACCESSION   DAONUO010000233 DAONUO010000000
VERSION     DAONUO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19491_length_3373_cov_3.657022"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK768_08285"
     CDS             <1..230
                     /locus_tag="PK768_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQA61010.1"
                     /translation="KRQLASFTLKPLTQRELEILSHLVDGKSYKEIANTLFLSLGTVN
                     TYVYHIYSKLNVSSRKELRHKLSVNIPDMSF"
     gene            673..1125
                     /locus_tag="PK768_08290"
     CDS             673..1125
                     /locus_tag="PK768_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61011.1"
                     /translation="MILLITLIACFLFASTAYALAVSFVSKSLPGSATIGYTYSFGAN
                     IEYDTHISGIPTPQLCKYFLEVPRKFAEPTFNYGSLTGFKFTSKTGEMTGEPIWETYQ
                     RFWLFGVEQRGAYFRARANYSGSSQSYVGTGPSTAPAAHYYRSVKVTY"
     gene            1211..2413
                     /locus_tag="PK768_08295"
     CDS             1211..2413
                     /locus_tag="PK768_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61012.1"
                     /translation="MSKIKIIMMLFKAYSVANRKKLIKDITTLTLLFSILLSFSILLL
                     VLRHTYMLAYAETKFDFILQGFSEGQIDGIAALDFVKTVFPVRIFSGEIVAGNNKIVA
                     DTYAADSFTGIEVSYFSNHVILKKDKDIMTNQNLNPILIDRDIARTLRTGIGGKVSKF
                     YGKEGREVIFTVAAICQPLENSLGRPVALILWRGEQRDIFYHDFGEGHEYSMMFIEVL
                     DRNRAHKYFQEEYVPKWYLEEYGPLTEEELLEMRRDSAVINRETELENLYDEMRYAPP
                     VIILISALGFIIFLIVLYREADKKVLMIEKEFFILNALGLPKRYFYIYYTVETMLMQI
                     PVLLGATLITRFIIYEFIFNMYIPGYLLLRYAALAFLLQIIAVCISGCFINLALKKKD
                     MASLLAKE"
     gene            2420..>3373
                     /locus_tag="PK768_08300"
     CDS             2420..>3373
                     /locus_tag="PK768_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61013.1"
                     /translation="MLSPLTIKYIVSELKYNPWQFIKVIILLIPSLAIIGMFSTLQGI
                     PYINQKIFLNNAGYQWIGFSEERITAEQSYRVIQANIAHMEPKNGGIHLKRSYPLELF
                     VQGGNGKSAFSETFFVQKNLYQGNLEDLSGRSNSIDIALSFNAVKRLKAFLGDTVTIY
                     MDSNGELIVYPATIKAILRTKYPDHHIGFGGLGLIGSDSNFEDFLQENNIPYQYVMFG
                     SGSTDIFQFDRKIYKKDQLAAASLLAISMPGSSNLGLYVLAAMGILAIYIIISRELNF
                     SANKRMRNIGIMRALGTPHNVIKTIFLVEQAIKIVFASLGAA"
BASE COUNT         1063 a          614 c          677 g         1019 t
ORIGIN      
        1 ccaaaaggca attagcctct tttactttga aacctttaac tcaaagagaa ttagagatac
       61 tttcccactt agtcgatgga aaaagctaca aagaaattgc aaatacctta ttcttgtccc
      121 ttggcactgt caatacctat gtctatcaca tatatagcaa gcttaatgtt agtagtcgga
      181 aagaattaag gcataaacta agcgtaaata tacctgatat gagtttttaa taaaatgaaa
      241 tcatatgctg tatatgattg acaaggaaac aactaacata taacctaatt ggtggagatg
      301 attaacgttt tgcaaaggaa gatgcttcat gaatgagcat ataagtattt atggttctaa
      361 agatcgccaa gtcttaaaat ggtggaacgg ttggatacaa tcttgttttt gcctatttta
      421 atgatgctat ttgataatgt ttaataagta atacttcagt acctttctaa gtagacctgt
      481 ccaatagcga gttaatcagt tcgcttatcc tgggcaagct gcgcaaaatg acattaataa
      541 atatagattg acatccaagc aaacatccag acacgtatat attatttaat tgaatttata
      601 taaatggcta tataaaccta attacattac ctgagtctac taatatacaa tgagatgaag
      661 aagaaaacta atatgattct attaattaca ctcattgcat gctttttgtt tgcttctaca
      721 gcttacgcat tagcagtaag tttcgtgtca aaatcactgc cgggatcagc aactattggc
      781 tatacttaca gctttggtgc aaacatcgaa tatgatacac acatctctgg aattcccact
      841 ccccaattgt gcaagtattt tcttgaagtg ccaagaaaat tcgcagaacc gacttttaac
      901 tacggttcct taacaggttt taaattcaca tctaagacag gagaaatgac cggcgaaccg
      961 atatgggaga cataccaaag attctggtta ttcggtgtcg aacaaagagg tgcttatttt
     1021 agagctcggg ctaattattc cggaagcagt caaagctatg ttggcacagg tccaagcaca
     1081 gcaccggctg ctcattatta tcggagcgta aaagtaacct attaaggctg cgaaaacaaa
     1141 aacaggttta tatagccact tacattataa aacaaatctg ttaaagcgtt ttaaagggtg
     1201 tactcatacg atgtcaaaga taaagatcat aatgatgctt tttaaagctt acagcgtcgc
     1261 caacaggaaa aagcttatta aagatattac caccctgacg ttgttgtttt caattttact
     1321 ctctttcagc atactgttat tagttttacg gcatacatat atgctagcct acgcggaaac
     1381 aaaatttgat tttattctcc aaggtttttc tgaagggcag atcgatggaa ttgccgcatt
     1441 agattttgtc aaaacggttt ttccagtcag aattttttct ggagaaattg tcgcaggtaa
     1501 caataaaata gtagccgata catatgccgc ggatagtttc acgggtattg aagtttcata
     1561 tttttccaac catgtcattc taaaaaaaga taaggatata atgaccaacc agaaccttaa
     1621 tcccatttta attgacaggg atattgcccg caccctgaga acaggtattg gagggaaagt
     1681 atcgaaattt tatggtaagg aaggaaggga agtcatcttt accgtggctg ctatttgcca
     1741 acccctagag aattctttgg gccgtcctgt cgcgctgatt ctctggagag gagagcaaag
     1801 ggacattttc tatcatgatt ttggtgaagg tcatgaatac agcatgatgt ttatcgaagt
     1861 ccttgaccgc aaccgtgctc ataagtattt tcaagaagaa tatgtaccta aatggtacct
     1921 tgaagagtat ggccctctga ccgaggaaga gttgctggaa atgaggagag actcggcagt
     1981 cattaacagg gagacggagt tggaaaatct atatgatgaa atgcgatacg cgccgcctgt
     2041 tataatcctg attagtgccc tgggctttat tatttttctt atcgttttat accgggaagc
     2101 agataaaaag gttctaatga tagagaaaga gttttttata ctgaacgcgc ttggtttacc
     2161 gaaacgttac ttttacattt attacactgt tgaaacaatg ctgatgcaaa tacctgttct
     2221 tttgggagca acccttataa ccaggtttat catatacgaa tttattttta acatgtatat
     2281 tcccggttac ctgctcctga gatatgctgc gctggccttt ttattacaga tcattgccgt
     2341 atgcatcagc ggatgtttca tcaacctggc gctgaagaag aaagatatgg catcgctttt
     2401 ggcaaaggag tagatcatga tgctcagccc gttaacaatt aaatatatcg tatctgaact
     2461 taaatacaat ccatggcagt ttattaaagt gattatcttg ctgattccct cactggcgat
     2521 aatcggtatg ttttcaacgt tacagggtat tccttatatc aatcagaaaa tttttctcaa
     2581 caatgcaggc tatcaatgga tagggtttag cgaagagaga ataacagctg aacaaagcta
     2641 tcgcgtaatt caagccaata tcgcccatat ggagccaaaa aatggaggga ttcacttaaa
     2701 aagatcttat cccctggaac tatttgtgca ggggggcaat ggtaagagcg ccttttcgga
     2761 aacattcttt gtgcaaaaaa acctttatca gggaaacctt gaggatctct ccggcagaag
     2821 caatagtatt gacattgctc ttagctttaa tgctgtgaaa agattaaagg cttttcttgg
     2881 agatacggtg accatatata tggattcaaa tggcgagctt atcgtttatc ctgctacaat
     2941 taaagcgatt cttagaacaa aataccctga tcatcatatc ggtttcggtg ggttgggatt
     3001 gatagggtcg gacagtaatt tcgaggactt tttgcaggaa aataatattc cgtatcaata
     3061 cgtgatgttc ggttcgggaa gcaccgatat attccagttc gatcgaaaga tatataaaaa
     3121 agatcagctt gccgccgcct cccttctggc tatcagtatg ccgggcagca gtaatctggg
     3181 actttacgtg cttgccgcaa tgggcattct ggcaatatat ataatcatca gcagggagtt
     3241 aaatttttct gcaaataaaa gaatgcgaaa catcggtatt atgagagctt taggcactcc
     3301 tcataatgta ataaaaacca tatttcttgt ggagcaagcc ataaaaatag tttttgcctc
     3361 gttgggtgcc gct
//
LOCUS       DAONUO010000234         3354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19618_length_3354_cov_3.717187, whole genome shotgun sequence.
ACCESSION   DAONUO010000234 DAONUO010000000
VERSION     DAONUO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19618_length_3354_cov_3.717187"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..114
                     /locus_tag="PK768_08305"
                     /pseudo
     CDS             1..114
                     /locus_tag="PK768_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
     gene            121..1713
                     /locus_tag="PK768_08310"
     CDS             121..1713
                     /locus_tag="PK768_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQA61014.1"
                     /translation="MILKVCSGDIKTSPRKIEYDGSIENAVKLLEQSIPISAKQAIRS
                     RWLALRLIEGDSKILDAVSYYCDMTTFQKYELKKKLEELDAILKTKFASSDEIKDAIV
                     SNIVLKAEEISRKVVSIQDEGYKKLDRQIDDILTSKLFGIPIMLVLLGFVFWLTISGA
                     NYPSALLAKGLFYVEDLLTEMCFSFGIPDWLHGAFVLGIYRTVAWVVSVMLPPMAIFF
                     PLFTVLEDLGYLPRVAFNLDNFFKKAHAHGKQALTMCMGFGCNAAGVIACRIIDSPRE
                     RLIATITNNFVPCNGRFPTLIALSTIFVAGSFNAFQTLIATLTMLTAIVSAVLVTLIV
                     SKILSCTVLKGLPSTFTLELPPYRKPQIGRILVRSLLDRTIFVLARAVAVAAPAGLVI
                     WLMANTSVSGMSILERSAKILEPFGRLLGMDGYILLAFILGLPANEIVIPIIVMSYTA
                     SGSLLELDSIESLRQLLLANGWTRLTAVCTMIFSLNHFPCGTTLLTIYKETQSKKWTV
                     ISFLVPTLVGIIACLGARHLTY"
     gene            1840..2028
                     /locus_tag="PK768_08315"
     CDS             1840..2028
                     /locus_tag="PK768_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HQA61015.1"
                     /translation="MKVYVDQDLCISCGLCIDICPAVFDWNDDGKADVIVDEVPADAE
                     DDAREAMESCPSEAIKEE"
     gene            2173..2814
                     /locus_tag="PK768_08320"
     CDS             2173..2814
                     /locus_tag="PK768_08320"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HQA61016.1"
                     /translation="MKKLEVMKRIIDCGIVAVVRAESADQALKIAEAVKAGGVEAIEI
                     TMTVPGAIDVIKELAKAYTKGEILIGAGTVLDSETARAAMLAGAEFIVSPYFNPDLVR
                     ICNRYQKVSMPGAMSIKEVVEVMESGADFVKFFPGSGFGPSMIKAILGPLPYAPIVPT
                     GGVSLDNVDKWIKAGCKAVGVGGELTKGAKKGDYKEVEETARKFVEAIRAARA"
     gene            2887..>3354
                     /locus_tag="PK768_08325"
     CDS             2887..>3354
                     /locus_tag="PK768_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQA61017.1"
                     /translation="MANKVVTFGEIMLRLTPPNHLRFVQADSFDVTYGGGEANVAVSL
                     ANYGEDAYFVTKVPDNPIGQSAINHLRRYGVNTQYIVKGGERLGIYFNEIGASQRPSL
                     VVYDRSNSAISQAQAGDFDWEKILEGAKWFHFTGITPALGDNVAECCLEAVKTA"
BASE COUNT          982 a          693 c          798 g          881 t
ORIGIN      
        1 atggatgagg ctaggaggaa gaaaatagaa attgatttaa aaggattgtc agaggtactg
       61 ggtgtgccgg ttgtaggcac taatgcgagg gatggagaag gccttgatga cttaaaggat
      121 atgattttaa aggtttgcag cggtgatatc aagacttctc cccgtaaaat tgagtatgat
      181 ggctcgatag aaaatgcagt gaaattactt gagcaaagta tcccgatttc agccaagcaa
      241 gcaattagaa gtcgatggct tgccctccgt cttatcgaag gcgacagcaa aatccttgat
      301 gcagtatcat attactgtga tatgacaact tttcaaaagt atgaacttaa gaaaaagctg
      361 gaagagctag acgctatatt aaaaacaaaa ttcgcaagca gcgatgaaat aaaagatgcc
      421 atagtttcaa atattgtatt aaaagcagag gaaatcagca ggaaagtggt aagcattcaa
      481 gatgagggat ataaaaagct tgaccggcaa attgacgaca tattaacttc caagcttttc
      541 ggcataccta ttatgctggt ccttttagga ttcgttttct ggcttaccat ttcgggagca
      601 aattatccat cggcgcttct ggcaaagggc ctgttttatg tagaagacct gctgaccgag
      661 atgtgtttta gcttcgggat accggactgg ctccacggcg cttttgtatt gggcatatat
      721 aggactgtag cctgggtggt atcagtaatg ctgcctccaa tggccatatt tttccccctt
      781 ttcaccgtgc ttgaagattt gggatactta cccagggtag cctttaacct ggacaatttt
      841 tttaaaaaag cccacgccca cgggaaacag gcccttacca tgtgtatggg ctttggctgc
      901 aatgcagcag gtgttattgc ctgcagaata atcgactcac cgagagaacg gcttattgcc
      961 accatcacaa acaattttgt tccctgtaac ggccgatttc ccacattaat agccctttca
     1021 accatattcg ttgccggctc ctttaatgct tttcaaacct tgatagcgac ccttaccatg
     1081 ctgacggcca ttgtatctgc agtacttgta acccttatag tatcaaaaat attatcctgc
     1141 accgtgctaa aaggcctgcc atccactttt acattggaac tgccccctta caggaagccc
     1201 cagatcggac ggattttggt gagatcactt ttagacagaa ccatatttgt cctggccagg
     1261 gcagtggcgg tggccgcacc ggcaggcctt gtaatatggc tgatggcaaa tacgagtgta
     1321 agtggaatga gcatcctgga acggagcgca aaaatcttgg aaccctttgg gcgactgctg
     1381 ggtatggatg ggtacatcct tctggcattc atccttggcc ttcccgccaa cgaaattgta
     1441 ataccaatta tcgtcatgag ctacaccgcc tcaggctctc tgctggagct ggactccatc
     1501 gagtcattga ggcagctctt acttgccaat ggctggaccc ggcttacagc agtttgcacc
     1561 atgatatttt ccctaaatca ctttccctgc ggaacgacat tgctgacaat ctataaagaa
     1621 acccaaagta agaagtggac agtaatatcc tttctagtcc ccacattagt aggaataatc
     1681 gcctgccttg gtgccaggca cctaacttat taacttattg aatgttgtgt aaataaaccc
     1741 ggatgccgtc aattttttcg tagagcttaa aaaaatattt gcattgcagg taatttgtga
     1801 taaaatatac aaagaaaaat atttgaggag gtattttcct tgaaagtata cgtagatcaa
     1861 gacctatgta tcagctgtgg actctgcatc gatatctgcc ccgcagtttt cgactggaat
     1921 gacgacggca aagcagacgt gatagtcgac gaagttcctg ccgatgctga agatgatgca
     1981 agggaagcaa tggaaagttg cccttcagaa gcaattaaag aagaataatt gctaaggaag
     2041 agaatctcat ctgtcatgaa tgagattctc tttttcttta taaactaaag aaggatttta
     2101 aacctttatg tagaataata taaatgatat tatgaaacac aatatcacat tatgaaacag
     2161 gaggtttgtc ccatgaagaa actagaagtc atgaaacgca tcattgactg cggtatcgtg
     2221 gcagtagtga gagctgaatc tgctgaccag gcacttaaaa tcgccgaagc agtaaaggca
     2281 ggcggcgtgg aagccataga gattacaatg acggttcccg gggccattga tgtcatcaag
     2341 gaactggcca aagcgtatac caagggagaa atattaatcg gagcaggtac ggtccttgac
     2401 tcagagactg ctagggcagc aatgctggcc ggagcggaat tcatagtaag tccttatttc
     2461 aaccctgatc tggtgagaat ttgcaacaga taccagaaag taagcatgcc aggcgccatg
     2521 tccataaaag aagttgtaga ggtcatggag tcgggtgcgg attttgtaaa attcttcccg
     2581 ggcagcggat ttggcccatc catgattaaa gcaatacttg gaccactgcc ctatgcaccc
     2641 atcgtcccca caggcggtgt aagccttgac aatgttgaca aatggattaa agcaggatgc
     2701 aaggcagttg gtgtaggcgg agaattaact aagggcgcca aaaaaggaga ttacaaagag
     2761 gtagaagaaa cggccaggaa attcgtagag gccattagag cggcaagagc atagcgttga
     2821 cttatgtcta gaaggcagat gttaatgttt ataattgcta acttttttaa aaggaaggtg
     2881 ttttaagtgg ctaataaagt tgttactttt ggtgaaataa tgctcaggct cacccctcca
     2941 aatcatttaa gatttgtcca ggctgactct tttgatgtga catatggagg cggcgaagct
     3001 aatgttgcag tgtcccttgc caactacggc gaagatgcat atttcgttac caaggttccc
     3061 gacaatccta taggccaatc ggccataaat cacctgagac gctatggagt aaacacccaa
     3121 tatatagtaa aaggcggcga acgcctgggc atttacttta atgaaatagg tgcttcgcag
     3181 cgtccctctt tggtagtgta cgaccgcagt aattctgcta tatcccaggc ccaggcggga
     3241 gattttgact gggaaaaaat attggaaggt gctaagtggt tccacttcac cgggattact
     3301 cctgcactag gtgataatgt ggctgagtgc tgcctcgaag ctgtaaagac cgcc
//
LOCUS       DAONUO010000235         3350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19645_length_3350_cov_4.352959, whole genome shotgun sequence.
ACCESSION   DAONUO010000235 DAONUO010000000
VERSION     DAONUO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19645_length_3350_cov_4.352959"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /locus_tag="PK768_08330"
     CDS             <1..1744
                     /locus_tag="PK768_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA61018.1"
                     /translation="FNKLLFFGGIFFIIVSAASQIAANAMLSPIIDTIVIGDIPQVVR
                     YLLIMGAIVLGISGSQYLGNLFMARLAQETVHKIREDMFSHMQKLPVSYFDKLAHGDL
                     MSTFTNDVDMLNQSLEQSVSQAIVAVITVAGSFAMMLVLSPILTLTVILMLGLMFAAV
                     KYVGRKSASNFRFQQAALADMNGYVEEIMSGQKVVKVFNYEERAIDKFLEKNEQLRRA
                     STQASTYGVMLMPIMGNLSFVLYALASMFGAFLVMKNSMTIGNIAAFLQYTRTISRPI
                     TMVSNQLNMLFAALAGAERIFDVLDEEAERDEGDVCLVRDGKNPPYWKVPKENGEYET
                     VPLKGYITLDNVNFGYEPGQRVLSNISLYAKPGQKIAFVGSTGAGKTTITNLINRFYE
                     IDEGSILFDGIDIRRIKKQDLRSTMSIVLQDVHLFEGTIADNIRYGRLDATDEEVVEA
                     AKLANAHYFIKNLPKGYDTMLTMDGQNLSQGERQLLSIARAAIANPTVLILDEATSSI
                     DTRTEKLIAEGMDKLMEGRTTFVIAHRLSTVRDANAIMVLEQGRIIERGDHEELMRQK
                     GRYYALNTGALELE"
     gene            2016..3023
                     /locus_tag="PK768_08335"
     CDS             2016..3023
                     /locus_tag="PK768_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA61019.1"
                     /translation="MRKKLVSIVALILILSMLLPLCAMAADDPVRLEEISERKPGQNV
                     KIEGSTDLAEVTVKVIRPSGSVMYINVLKVTEGKFSDGFTLPGDAETGEYTVVVGQGD
                     IVGKVNFKVKDSGEEPGGPGGGGGSSKPKEEPEEPTPVKKTFEDIDDYPWAKEAIETL
                     ATLGIIQGTSETTFDPGSNITRADFITLLVRMLKMFDMVPDDGSDSNFDDVQEGMYYY
                     EPIGIAKKLGIVQGIGANKFNPSGQITREDLMVMVARALKIANKITTTGSYADISRFN
                     DASEVADYAVESIAILVKEGIIVGNPNNTINPKGNATRAETAVILYRVYLKYFAAEQD
                     S"
     gene            complement(3077..>3350)
                     /locus_tag="PK768_08340"
     CDS             complement(3077..>3350)
                     /locus_tag="PK768_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61020.1"
                     /translation="SRLRKLGVEMLTQAKVTAIEDDRVVYEKGGKEYTVEGVDTVVLA
                     AGMESVNELADELKALGKPIHVMGDASNVSIIMEGLADVYDKLINV"
BASE COUNT          959 a          790 c          806 g          795 t
ORIGIN      
        1 attcaataaa ctgctgtttt tcggcggcat attcttcatt atagtctcgg cagcctcaca
       61 gatagccgca aatgccatgc ttagccccat catcgatacg atcgtgattg gcgacatccc
      121 gcaagttgtc aggtacctgc tgataatggg agcgatcgta cttggcattt ccggaagcca
      181 gtatttgggg aacctcttca tggcgaggct ggcccaggag acggtccaca agattcggga
      241 agacatgttt tcccacatgc aaaagctgcc cgtttcctat tttgacaagc tggcccacgg
      301 ggatttgatg tccaccttca ccaacgacgt ggatatgttg aaccagtccc tggaacaaag
      361 cgtatcccag gcaatcgtcg cagtcataac cgtggcaggc tcttttgcca tgatgctcgt
      421 attaagcccg atactcacat tgacagtaat cctgatgctg gggttaatgt ttgctgcggt
      481 aaaatacgta ggccgaaagt cggctagcaa ctttcgcttc cagcaggctg ccctggcaga
      541 catgaacggc tatgtagagg agatcatgtc ggggcaaaaa gtcgtgaagg tatttaacta
      601 tgaggaaagg gcaattgata aattccttga gaaaaacgaa cagcttcgcc gggccagcac
      661 ccaggcctct acttacggtg tgatgctcat gcccatcatg ggcaacctgt ccttcgtgct
      721 gtacgccctg gcctccatgt tcggcgcctt tttagtgatg aaaaattcaa tgaccatagg
      781 caacatagcg gcctttctgc aatataccag gaccatatcc cggcccatta ccatggtatc
      841 aaaccagctc aatatgctct ttgcagccct ggcaggggcg gagcgcatct ttgacgttct
      901 ggatgaagaa gcggaaaggg atgaaggtga tgtctgcctt gtaagggacg ggaaaaatcc
      961 cccctactgg aaggtcccca aggaaaacgg ggaatacgaa acggtgcccc ttaaaggcta
     1021 tataacttta gacaatgtta acttcggtta cgagccgggg caaagggttc tcagcaacat
     1081 aagcctttat gcaaagccgg gccagaagat cgccttcgta ggctcaaccg gtgccggcaa
     1141 gaccaccatc accaatctca taaacaggtt ttacgagata gatgaaggca gcatactctt
     1201 tgacggcata gatatcaggc gcattaagaa gcaggatctg agaagcacca tgagcatcgt
     1261 ccttcaggat gttcaccttt tcgaggggac aatcgcggac aatatcaggt acggtaggct
     1321 ggacgccacg gatgaggaag tggtggaagc ggcaaagctg gccaatgccc attactttat
     1381 caaaaacctc cccaagggct acgataccat gctgacgatg gacggccaaa acctttccca
     1441 gggtgagcgc cagctcctct ccattgccag ggcggcaatt gccaatccca ccgtcctcat
     1501 cctcgatgag gccacatcat caatagatac caggacggaa aaactcatcg cagaaggcat
     1561 ggacaaactg atggagggca ggactacttt cgtaattgcc caccgcctgt caacggtccg
     1621 ggatgccaac gcaatcatgg tattggaaca ggggagaata atagagcggg gagaccatga
     1681 ggaactgatg aggcagaagg gccgttacta cgccctgaac accggtgccc ttgaattaga
     1741 ataaagataa aacgagagat taggcccgta aagggccttt ttgtcaaaaa gcggcaagct
     1801 ttttatagcc caattctaat ccagttttgc tgcttgacaa ctcctgccat tcagatctaa
     1861 ttaaagtggt tgtaagaata tttatgatat taaaaccccg aaaactgcca ttttctacat
     1921 tttccccgaa gaaaagccaa ctctcccttg attctcattt gcttttttat ttataataga
     1981 attaataaaa aatgtaagaa aggaatggta ggccaatgag aaaaaaatta gtcagtattg
     2041 tcgcactaat tctcattttg tcgatgctct tgcccttatg tgccatggcc gctgacgacc
     2101 ccgttaggct tgaggaaatc agtgaaagga agcccggcca aaatgtaaaa atagagggaa
     2161 gcaccgattt agcagaagta accgtaaaag taattaggcc tagcggatca gtaatgtaca
     2221 taaacgttct aaaagtgaca gaaggcaaat tttctgacgg gttcacactg cctggggatg
     2281 cagaaaccgg cgaatatacg gtagtagtag gtcaaggaga tatagtaggt aaggtgaatt
     2341 ttaaagtaaa agattccggt gaagaacctg gcggtcccgg cggtggcggc ggttcatcaa
     2401 agccgaagga agaacctgaa gagccaacac ctgtaaagaa gacctttgaa gacattgacg
     2461 attacccctg ggcaaaggaa gctatagaaa cactggccac tttaggaatc atacagggaa
     2521 cttcagaaac aacctttgat cccggcagca atatcacaag ggcagacttc ataaccctat
     2581 tagtgagaat gctgaaaatg tttgatatgg ttcctgacga tggctctgat tcaaactttg
     2641 acgatgtaca ggaaggcatg tactattatg agcccattgg catagccaaa aagctgggca
     2701 tagtccaggg tatcggagca aacaagttta acccatcagg gcaaattaca agggaagacc
     2761 tgatggtaat ggtagccagg gcactgaaga tagcaaacaa gattacgacc acgggaagct
     2821 atgctgacat cagcagattt aacgatgcct cagaggttgc cgattatgcc gtagaaagca
     2881 ttgcaatcct ggtcaaagaa ggcattatag tcggaaatcc caacaatacc attaacccca
     2941 agggcaatgc cacaagggcc gagacggccg taatcctcta cagggtgtat ttgaaatact
     3001 ttgcagctga acaagacagt tgaggaccat atgcaaagca ccaacccttt tcggttggtg
     3061 ctttgtttta aatcttttac acattgatta gcttgtcata aacgtcggcc aagccttcca
     3121 tgattatact tacattgctt gcgtcgccca ttacatgaat cggcttgccc agggctttca
     3181 gttcgtcagc cagttcattc acactttcca ttccagccgc gaggacaacg gtgtcgactc
     3241 cctctacggt gtattctttt ccgccttttt cgtaaactac tctgtcgtct tctatagccg
     3301 ttactttagc ctgggtaagc atttctactc ccaattttct cagcctagaa
//
LOCUS       DAONUO010000236         3347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19673_length_3347_cov_3.919198, whole genome shotgun sequence.
ACCESSION   DAONUO010000236 DAONUO010000000
VERSION     DAONUO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19673_length_3347_cov_3.919198"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /gene="amrB"
                     /locus_tag="PK768_08345"
     CDS             complement(<1..603)
                     /gene="amrB"
                     /locus_tag="PK768_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQA61021.1"
                     /translation="MAILSCYLMPHPAIMIEEVGGRETQKVAASVKAADTVAREIKEL
                     APDTIVIISPHGPVFYDALCIYDFPLRGSLADFGVPQIKMDFVRDDELKEELLTRAEH
                     KDIPATTAGKASFFGFGINKKLDHGVTVPLYFVTKHFKEFKLLPIAFGMLPYEELYAF
                     GRLIRESAEALAKKVVVIASGDLSHRLTYDAPAGYDVNGRI"
     gene            complement(629..1801)
                     /locus_tag="PK768_08350"
     CDS             complement(629..1801)
                     /locus_tag="PK768_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA61022.1"
                     /translation="MKIHDEIKNFVIVLAATITFISTYYIMMPVIPDHMLYLGFDNLT
                     IGTVMGLFSISSMIARPVGGVMINSYGSKKVMFISIILFFLTPFLLKLPYAIPGLSLT
                     LLIYGFTVGAFTVASATFSADIASSENLTRFMGYNSIAVIIAKGLAPAAGIKLMEGYG
                     FTAAVWGTVIVAAIAMVLLFFSSDVSTSKQSQGNSSFLSVLLNRSVYLPTIVLFCGMV
                     TFGAISAMLPLFAVAKGITGTEYFFIINTAVVVATRLVIGRWSNRYLEELVAVALAVL
                     TLSFIFMSFVDSFPKLIVAAMIYGVGFALLFPLMTSLLVINIYEVSRSMALGVLTAAF
                     DLGVAAGVMLGGFSKYIDFKWLYLYLAVLPFIGFCLYQCVYRPFIREQRMKYKGAS"
     gene            complement(1911..2993)
                     /locus_tag="PK768_08355"
     CDS             complement(1911..2993)
                     /locus_tag="PK768_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQA61023.1"
                     /translation="MEKFAFMIHPIELSDIYRKLKFMEKLPESAVEGIIKRFPPMKIS
                     HITGVRSEHSEAEGYFVACPLTTKQMIELPEEYVMDKIIKTGKLAEKMGAKILGLGAF
                     TAVVGDAGITVAKNLNIPVTTGNSYTVATAIEGTKKAAEIMGKSIKDAQVLVIGAAGS
                     IGNVCAQILAKDCKYMTLAGRHTEKLERVADKILKDTGLSVMITSNVKKAVRKADVII
                     TVTSSLDAIIEAEDLKPGAVVCDVSRPRDVSKEVAQKRSDVLIIEGGVVEVPGDVNFN
                     FNFGFPPKTAYACIAETMMLALEGRYESFTLGRELTVEQVEEISRIAEKHGFKLAGFR
                     SFERAVTEEQIEKIKENARRKIERAC"
     gene            complement(3015..>3347)
                     /locus_tag="PK768_08360"
     CDS             complement(3015..>3347)
                     /locus_tag="PK768_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HQA61024.1"
                     /translation="PNPKFGRFYNDADVIAGDFHFIKQHLPLDISGKIILTNTVTADD
                     VALLRERGARMLITTTPKLNGRSFGTNVMEGVLVSLSEKPYKSLTPADYNELLDRIGF
                     SPRIEVLN"
BASE COUNT          847 a          836 c          742 g          922 t
ORIGIN      
        1 aatccttccg tttacatcat atccagcggg agcatcataa gtgagtctgt gggacaggtc
       61 tccgctggct atgaccacca ctttcttggc tagggcttcc gctgattctc ttatgagtct
      121 gccgaaagca tacagctcct cgtagggcag catgccgaag gctatgggaa gcagtttaaa
      181 ttccttaaag tgcttggtga caaagtatag gggtaccgta actccatggt ccagtttttt
      241 gttaatgccg aagccaaaga aactggcttt accggctgtt gtagcgggta tatccttgtg
      301 ctccgccctt gtcagcagct cctcttttaa ttcatcatcc cggacgaaat ccatctttat
      361 ctggggtact ccgaagtctg caaggctccc cctcagcgga aaatcgtaga tgcataaggc
      421 atcgtaaaaa acaggaccgt ggggggaaat gatgacaatg gtgtcaggag caagttcttt
      481 tatttctctc gccacagtat cggctgcctt aaccgaggct gcaaccttct gggtttccct
      541 gcctcctacc tcttctatca taatggcggg atgcggcatc aggtaacagg acagtatagc
      601 cacagtaaaa cctcctttcc tatatatttt agcttgcacc cttgtacttc atccgctgct
      661 cacgtataaa gggcctatac acgcattggt acaggcaaaa gcctataaag ggcagcacag
      721 caagatagag gtacagccat ttaaagtcga tgtacttgct aaagcccccc aacatgactc
      781 ctgcggccac gcctaagtca aaggcagccg tcagcacgcc tagggccatg ctccttgata
      841 cttcgtaaat gttaatgacc agaagtgagg tcatgagcgg gaataggagc gcgaagccca
      901 ctccgtaaat cattgccgcg acgatgagct ttggaaaact atcaacgaaa ctcatgaata
      961 taaatgaaag ggtcaggact gccaaagcta ccgccaccag ttcttccagg tagcgattgc
     1021 tccacctgcc gattaccagc cttgtggcaa ccaccaccgc agtatttatg ataaagaaat
     1081 actccgttcc ggttatccct ttggccacag caaaaagggg cagcatggcc gaaatggcgc
     1141 caaaggtaac catgccgcaa aagagcacaa tagttggcag gtacacgctg cggtttaaaa
     1201 gcacgcttag aaaacttgaa tttccctggc tttgtttgct agtgcttaca tctgatgaga
     1261 aaaatagtaa caccattgca attgcggcaa caattacagt cccccagacg gcagcggtaa
     1321 aaccgtatcc ttccatgagc ttaattccgg ccgcaggggc aaggcctttt gcgataataa
     1381 cagcaatgga attataaccc ataaagcggg tcaggttttc agaagacgcg atatcagcac
     1441 taaaagttgc agaggccacg gtaaaggctc caaccgtaaa gccgtaaatc agcagggtaa
     1501 gggaaaggcc aggtattgca tacggcaatt ttaacaggaa gggggttaag aaaaatagta
     1561 ttattgatat aaacatcact tttttgctgc cataggagtt tatcataact ccgccgaccg
     1621 gccttgcaat catggagctt atagaaaaaa ggcccattac ggtgccgatg gtcaagttgt
     1681 caaagccgag atacagcatg tggtcgggta tgacgggcat cattatgtaa taggtggaaa
     1741 taaaggttat agtggcagct aggactatca caaagttctt tatttcatca tgtattttca
     1801 atctgatctc tccctgttac aattgtctgg taattgttat tttatcatat agctctgcaa
     1861 aaagtaagct gtgcttgcaa aaaagcccat ctgaaataga tggactttgt ttaacaggct
     1921 ctttctattt ttcgccttgc attttccttg attttttcaa tttgttcctc cgtaacagcc
     1981 ctttcaaagc ttcgaaaacc tgccagttta aagccgtgct tctcggctat cctgcttatt
     2041 tcttctacct gctctacggt cagctctctt cccagggtaa agctctcgta gcggccttcc
     2101 agggccagca tcatggtctc agcgatgcag gcatatgccg tctttggtgg aaagccgaaa
     2161 ttgaagttga aattcacatc accggggact tccacaacgc ctccctctat aatcaataca
     2221 tcactccgct tttgggccac ttcctttgac acatctctag gccttgacac atcacatact
     2281 accgcaccgg gctttaagtc ttccgcctct attatggcat ccaggctgct ggtaacagtg
     2341 atgatgacat cggcctttct gacagccttt ttcacatttg atgtgatcat caccgaaagg
     2401 cccgtgtcct ttaatatttt atcagcaacc ctttccagct tctcagtgtg tcttccggct
     2461 agggtcatgt atttgcaatc tttggcaagg atttgagcac agacgttgcc tatggaaccg
     2521 gcagctccta ttaccaggac ctgggcatct ttgatgcttt ttcccatgat ctccgcagcc
     2581 tttttggtgc cttcgatggc cgttgccacc gtatagctgt tgcccgttgt caccgggata
     2641 ttcaggttct tggcaacagt aatacctgca tctcccacta cggccgtaaa cgctccaagg
     2701 cccaggatct tagcccccat tttttctgcc agcttgccgg tcttaattat tttgtccatt
     2761 acatactcct ccggcagctc tatcatttgc ttggtagtaa ggggacatgc tacgaaatag
     2821 ccttcggctt cactgtgctc acttcttacg cccgttatat gagaaatctt catcggcgga
     2881 aatctcttga tgattccctc gacggcgctt tcaggcaatt tttccatgaa cttaagcttt
     2941 ctgtagatat cagagagttc gatgggatgt atcataaatg caaatttttc cattttcaca
     3001 cctcgaattt tattttaatt taggacttct atccgcgggc taaatccgat cctgtcaagt
     3061 agctcgttgt aatcggctgg agtcagtgat ttatacggct tttctgaaag ggaaaccagc
     3121 actccttcca ttacattggt cccgaaagac cttccgttga gcttcggtgt agtcgtaatt
     3181 agcatcctgg caccgcgttc cctgagcagg gccacgtcat cagcagtcac cgtatttgtc
     3241 aggatgatct tccccgaaat atccaggggc agatgctgct ttatgaaatg aaagtctccg
     3301 gcaatcacat cggcatcgtt gtaaaagcgg ccaaatttgg ggttggg
//
LOCUS       DAONUO010000237         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19873_length_3318_cov_3.319644, whole genome shotgun sequence.
ACCESSION   DAONUO010000237 DAONUO010000000
VERSION     DAONUO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19873_length_3318_cov_3.319644"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PK768_08365"
     CDS             complement(<1..962)
                     /locus_tag="PK768_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQA61025.1"
                     /translation="MSLLKKLEMTKKHTESTALEQGQSFTHFARDPYGHVKEELHGKI
                     IEELKNLKSPEREDKRHVRAYIEKLVNDNIESGAEHIPRLDRMRIADELFDEIMGYGP
                     ITPLLNDPEISEIMVNGPDQVYVERKGKIELTEVFFRDDAHVMNIIDRIVSPLGRRID
                     ESMPMVDARLPDGSRVNAIIPPLSLVGPVITIRKFSQDPLTIEDLIQFNTLTPEMARF
                     LEACVKSRLNIVVSGGTGSGKTTTLNVLSSFISPTERIITIEDAAELQLHQPHVVTLE
                     SRPPNIEGKGAISIRDLVRNALRMRPDRIIVGEVRSGEALDMLQA"
     gene            complement(984..2204)
                     /locus_tag="PK768_08370"
     CDS             complement(984..2204)
                     /locus_tag="PK768_08370"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQA61026.1"
                     /translation="MTPITVLIADDIAATREDIKRLLYFEEDIEVVGEAGDGEEAVIL
                     ASELKPDVVLMDVNMPRVDGIEATERISVNVPQCAIIIISIQGEQEYLRRAMAAGARD
                     YLVKPFSAQDLAKAIYRVSESQRKRNIFLSPSLSAVSTSSIRAKKGTIISFFCTKGGV
                     GKTTLACNTAVALAQDYKKKVVLVDLDLSSGDVAVMLNLNPKNTIADMVQEQDTLDSP
                     LVESFLVQHLSGLKVLPAPLSPEHAELVKAEHIQQLLKILKDNYECVIIDTAPVYTDI
                     NLNVLEASDRILLPLNQDLTTLKHVKKAQEILAALNYDSKIRTVLSQHSSEGLKIKEL
                     QKNLGISLSAIVPEDFKTVRNAINKGVPFVMNQQHTKVAQSIGKLIEALDIAKPREAA
                     ANPKPSFITRMFSL"
     gene            complement(2217..2921)
                     /gene="cpaB"
                     /locus_tag="PK768_08375"
     CDS             complement(2217..2921)
                     /gene="cpaB"
                     /locus_tag="PK768_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HQA61027.1"
                     /translation="MKNKFVLLLAIVLGLVAAFGTFKYMEGLEQSYKLSGNYTQIAVA
                     KQHIRPKTVLTKEMLELKEMPAEYVLPGTVVDLKDAEGKITQGDIFPGEVILSSKLTS
                     KDDPAAGLSAKIEANKRAISIPANKVNALHGLISVGDFVDVLATFDVEVSDKRESYTS
                     TLAQHVPVLAINSSLQTSASEKLELETVTLMVEPVHAQQITMAMEKGSIQLVLRAPDD
                     KEVQSITSTKIEQLLR"
     gene            complement(2940..>3318)
                     /locus_tag="PK768_08380"
     CDS             complement(2940..>3318)
                     /locus_tag="PK768_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61028.1"
                     /translation="GYKGTIKVGDWIETEPGNMSGPTFDGVTYRINSCQHTPRCSIDR
                     YDRNCPMVMIVPIYEPSSLQGRSQVKIVGFGAFLLKGVSGKGTNSRVSGYFLETIPPD
                     GMNYTIDPNQDDYGLRTAKLISE"
BASE COUNT          712 a          799 c          804 g         1003 t
ORIGIN      
        1 gcctgcagca tgtccagggc ctctccgctt cggacttcgc cgacgattat ccggtctggc
       61 ctcatgcgaa gggcgttcct tacgaggtcc cgaatgctaa tggctccctt gccttctatg
      121 ttagggggcc ggctctcaag ggtcaccaca tgaggttggt gcagctgtag ctctgcagca
      181 tcttcgatgg ttatgattct ttcggtgggg gaaatgaagg atgagaggac gtttagggta
      241 gtggttttac cgctgccggt tcctccggat actacgatgt tcagcctgct tttgacacag
      301 gcctctaaaa agcgggccat ctcgggagtc aaagtattaa actgaataag atcctctatc
      361 gttaaagggt cctgggagaa ctttcgtatt gtaatgacgg gccctaccag ggaaagggga
      421 ggaattattg cgtttacgcg ggaaccgtca ggaagcctcg cgtcaaccat cggcatgctc
      481 tcatctatgc gcctgcccag gggtgaaacg atcctgtcga tgatattcat gacatgggca
      541 tcgtccctga agaatacttc ggttaattct attttgccct ttctttcgac gtacacttgg
      601 tccggcccgt tgaccattat ttcgctgatt tccgggtcat ttagcagggg cgttatgggg
      661 ccgtatccca tgatttcatc aaagagctca tcggctattc tcatgcgatc aaggcgaggg
      721 atgtgttccg ctccgctttc tatgttgtcg ttgacgagtt tttctatata agctctcaca
      781 tgccttttgt cctccctttc gggggacttt aaatttttca gttcttctat aattttgcca
      841 tgtaattctt ccttgacatg accgtaaggg tctcttgcga agtgagtaaa gctttgccct
      901 tgttcgaggg cggtgctctc ggtgtgtttt tttgtcattt caagtttttt caacaatgac
      961 ataagggtca ctcctgcttt gaattaaagg ctaaacatcc tggttatgaa ggatggtttt
     1021 gggtttgccg ctgcttcccg gggctttgca atatccaatg cttcaatcag cttgccgatg
     1081 ctttgggcaa ctttagtgtg ctgttggttc atgacaaagg gcacgccctt gttgatggcg
     1141 tttcttacgg tcttaaaatc ctccggcact atagcagaca aagaaatccc gaggtttttt
     1201 tgaagttctt tgatctttag accttcagaa ctgtgttgac ttaaaaccgt cctgattttc
     1261 gagtcatagt ttaaggccgc gagtatttcc tgggcttttt ttacgtgttt cagagtggtt
     1321 aaatcctggt tcaggggaag cagtatcctg tcactggctt ccaggacatt taaatttata
     1381 tcggtataga ccggagccgt gtcgatgatt acacattcgt agttgtcttt taagattttc
     1441 aagagctgct gtatatgttc tgcttttacc aattctgcat gttcgggact caagggggcg
     1501 gggagcactt taagacccga cagatgctgg accagaaagc tttccaccaa tgggctgtca
     1561 agggtgtcct gttcctggac catatcggct atggtgtttt tggggtttaa attcagcatg
     1621 acggctacat ccccggaact aaggtccaaa tccaccaaga ctactttctt tttatagtcc
     1681 tgggccaagg ccaccgccgt attgcaggcc agtgtggttt tccccacacc ccctttggtg
     1741 cagaaaaaac ttatgattgt gcctttttta gcccggatgc ttgaagttga taccgcagac
     1801 agggaagggg agaggaagat gtttcgcttt cgctggcttt ccgagacgcg gtagattgct
     1861 ttggccaggt cctgggccga aaagggcttt accaggtaat ccctggcccc tgcagccatg
     1921 gccctcctta aatattcctg ttcgccctgg atggagatga tgattatagc acactggggg
     1981 acattgacgg aaatccgctc ggtggcctca attccgtcga ctctcggcat gtttacatcc
     2041 atgagcacta catcgggttt tagctcactg gcaagtatga ccgcctcttc gccatcaccg
     2101 gcttctccca cgacttcgat atcctcttca aagtaaagaa ggcgctttat atcttcccgg
     2161 gtggcggcta tgtcatcggc tattaatacg gtaatgggtg tcatgataag acctccttaa
     2221 cgcagcaact gctcaatttt tgtagatgtg atactttgca cttctttgtc atcgggagcc
     2281 cgaagcacca gctgtatact tcccttttcc atggccatgg ttatctgttg ggcatgcacc
     2341 ggttctacca ttaaagttac ggtttccagt tctagttttt cggaagcgct cgtctgtagg
     2401 ctgctattta tggccaaaac cggcacgtgt tgggcaaggg tggaagtata ggattctcgt
     2461 ttgtccgata cttcaacatc aaaagtagct aatacgtcca cgaaatcccc tacgctgatc
     2521 aagccgtgca gggcatttac tttattggcg ggaatggata tggccctttt attggcctca
     2581 atttttgcag aaagccctgc agccgggtca tctttgcttg tgagtttgct gctcaggata
     2641 acctctccgg ggaaaatatc cccttgcgtg attttgcctt ctgcatcttt taggtctact
     2701 acggtacccg gcaagacgta ttctgcgggc atttctttaa gctccagcat ttccttggtc
     2761 aggaccgtct taggcctgat atgctgtttt gcaactgcta tctgggtgta gttacccgat
     2821 aacttatagc tttgttctaa accctccatg tatttaaagg tcccgaaagc tgctacaaga
     2881 cctaaaacta ttgcaagtaa caatacgaat ttatttttca tacatatctc tccactaaat
     2941 tattctgaaa tcaattttgc cgtccgcaga ccgtaatcgt cctggttagg atctatggta
     3001 taattcatac cgtcaggtgg gatcgtctcc agaaaatagc cggaaaccct gctgtttgtg
     3061 ccctttccgc tcacgccttt cagaaggaat gcgccgaaac ccactatttt aacctggctc
     3121 ctgccctgca gggaacttgg ctcgtaaatg ggaacgatca tgaccatcgg gcagtttcta
     3181 tcgtaacggt caatggagca tctgggggtg tgctggcaac tgtttatgcg gtaagtcacc
     3241 ccgtcgaagg tgggtcccga catgtttccc ggctcggtct ctatccaatc cccaactttt
     3301 atagtccctt tgtagcca
//
LOCUS       DAONUO010000238         3305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_19966_length_3305_cov_4.017231, whole genome shotgun sequence.
ACCESSION   DAONUO010000238 DAONUO010000000
VERSION     DAONUO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19966_length_3305_cov_4.017231"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK768_08385"
     CDS             complement(<1..217)
                     /locus_tag="PK768_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HQA61029.1"
                     /translation="MEKYIIDLKDNRACELSISGGKGANLAKLHNIPGIPVPDGFIVS
                     AQCYSDFVLSMPEVRKLIVQLPEITADQ"
     gene            complement(232..822)
                     /locus_tag="PK768_08390"
     CDS             complement(232..822)
                     /locus_tag="PK768_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQA61030.1"
                     /translation="MPKETFYNLSEEKRQRIFQAAVSEFASHKFSEASLNRIVKAAKI
                     PWGSFYQYFEDKEDLYLYVIKEMSKYKWDALKQAGVENMAGDFFDTIREKIAALFELR
                     KHNPDYAQIALLQEMDDDDFVRKVRGDSTKRWKQIIERDKKRGIIKSEIDADVMIDMF
                     YNYVLLNFYKVGLDEEKFMATLDEAIKIMREGVSVK"
     gene            complement(1066..1662)
                     /locus_tag="PK768_08395"
     CDS             complement(1066..1662)
                     /locus_tag="PK768_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61031.1"
                     /translation="MGDDSMRLTILKKYDKQLGFVFLLLIVLFIFLAMTNKSFFNWAY
                     ERHQNLLSWYIRPLFIIPFCYFAYKKSWAGIMGTMFMVLTSMFWFPKPAEVSDQVVEF
                     LEMEKEYLTGEWTLPKVLIALLIPASLSALATAFWKRNLWIGVAVMVFIALAKMSWSV
                     AFGGESGKTVFVPAMVGLLICILLIYIGFRRMEKRKNN"
     gene            complement(1943..2629)
                     /locus_tag="PK768_08400"
                     /pseudo
     CDS             complement(1943..2629)
                     /locus_tag="PK768_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393244.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2723..>3305)
                     /locus_tag="PK768_08405"
     CDS             complement(2723..>3305)
                     /locus_tag="PK768_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA61032.1"
                     /translation="LILQENEGEPYVEKVRQELERISRGLNMALNMARLTSFEHDFAV
                     ETVLLHSIVTEVINEQKRYFIRKRVYPEVRIDSNVTVKSDKKWLKFIVEQLLVNAIKY
                     TEGENKKVIVSACDSEEGKVLEVKDQGVGIPKKDIRRVFEPFYTGENGRRFGESTGMG
                     LYLVSKVCKKLGHRIEIESKPGEGTSVRIIFSG"
BASE COUNT          953 a          773 c          514 g         1065 t
ORIGIN      
        1 tttggtctgc ggtaatctcc ggaagctgaa caatcaattt tctcacttcc ggcatagaca
       61 aaacaaaatc gctgtaacac tgagcactaa caataaatcc atcgggtaca gggatacctg
      121 ggatgttgtg cagcttggct agattggcgc ctttcccccc agaaatagac agttcgcacg
      181 ccctattgtc ctttaggtca ataatatatt tttccacttg cgatcccctc cttattttac
      241 acttacacct tcccgcatta tttttatggc ttcgtccagt gtggccatga atttttcttc
      301 atccagcccg accttgtaga aattcagcaa gacataattg taaaacatat ctatcattac
      361 atcagcatca atctccgatt ttattattcc tcgtttctta tcccgttcaa tgatctgttt
      421 ccagcgtttt gttgagtctc cccgcacctt ccgaacaaaa tcatcatcat ccatttcttg
      481 cagcagagca atctgggcat agtcagggtt atgctttctc aattcaaaga gtgctgcaat
      541 tttttcccgt atcgtgtcaa aaaaatctcc agccatgttt tcaacacccg cttgtttaag
      601 ggcgtcccat ttgtattttg acatttcctt tattacgtaa agataaagat cttctttatc
      661 ttcaaagtat tgataaaaac tgccccatgg tatttttgca gctttgacaa ttcgattaag
      721 agatgcctcg ctgaacttat gcgatgcaaa ttcgctaacc gcagcctgaa atatccgctg
      781 cctcttttct tcactcaggt tgtagaaggt ttcctttggc ataatcacac ctccatctag
      841 caatatgaga aggttctcgt ataagattat aacataatat ttcgcaaatg agaagcctct
      901 catatgaaaa gtttcgtctt atcctaacat ttatatcaaa aaagcaacgc tacgaaacta
      961 tatgtaacgt tgctttttta caaatttcca ggtaacgttc ttattcttaa agttgtttgt
     1021 aattctttat aggaattgac tctgcctcca tacctttagc ggttgttaat tattctttct
     1081 cttttccatc cttcgaaatc ctatatatat caatagaata catattaata atcctaccat
     1141 tgccggaaca aatactgttt tccctgattc gcctccgaaa gctacgctcc agctcatttt
     1201 agccaatgct ataaaaacca ttactgctac tccgatccac aaatttcgtt tccagaaagc
     1261 agtagcaaga gcagaaaggg acgctggtat caagagtgct attagaactt taggcaatgt
     1321 ccattcacct gtgagatatt ctttttccat ttctagaaat tcaacaacct gatcgctgac
     1381 ctcagcaggc tttggaaacc aaaacatact cgttaaaacc ataaacatag tccccataat
     1441 tccagcccaa ctcttcttat atgcaaaata gcagaaagga ataatgaaca gtggccgtat
     1501 ataccagctg agtaggttct gatgccgctc ataggcccag ttaaaaaagc ttttatttgt
     1561 catagctaaa aatataaata aaactattag gagaagaaat acaaatccca gttgcttgtc
     1621 atatttcttt aagatagtta acctcatgct atcatctccc attttatttt tccctatatc
     1681 actctataga aatgctgcac tgaaaataaa cagagccgct gcccgagaaa ctaaattcat
     1741 cggttcggct ctttttgtac aacctcgatt taaccaatac ttcgatgctt aaagccaata
     1801 atccctaacc atgtaaatgc tgctgcaaaa actatcaaca caatcagcgg cacaagtgga
     1861 agctcatgga ttgggaaaat gtaatgggca taggcgaaag gcgtccattg cattgcagac
     1921 cattcgacga ttagcggctt tcaccacgat gaatctcatt aaagaactgg ccatccttta
     1981 taaatatcac ccgatgacaa taactagctg acatgggatc atgagttacc attacagtgg
     2041 ttactcctat ttccctgttg atgctttcaa atagctccat aacatctttg gcagctttag
     2101 aatcaagatt tcccgtaggc tcatctgcca gcagaagtaa aggtgagtga ataattgctc
     2161 gagctatggc agcccgctgg gcctgtccac ctgatacttc ataggttctt ttgtcgagaa
     2221 tactatctat attaagcctt gaagctactt cctttaatta tttatccata gtagaaactt
     2281 tagctccatc cagtgtcaaa ggaattacaa tattttcacc gatggttaat gtatcaatta
     2341 aattaaaatc ctgaaataca aatccaagct tctttcgcct gaaaaatgcc agctttgaat
     2401 tatccagttt ataaggattt tcaccattta tcaacacctc tccagatgta ggggggtcta
     2461 tggtggagat gacatttagg agagttgtct ttccacttcc agaaggcccc ataattccaa
     2521 caaattcacc tttttcgata gttaagttga tatctttcag tgcacagaat tgaacctttt
     2581 ctccatatat ttttaaaata tgttttaact ttaaaatctc caattccaaa caccctttcg
     2641 gcttttttta actttatttt aaggtatccg tgcaccgcct gctatcgact attattacac
     2701 caaccttaca ttaatgtaat atttaacctg aaaagataat cctaacagaa gtcccttccc
     2761 ccggttttga ttctatttct attcgatgtc ccaatttttt gcacactttg ctgacaaggt
     2821 acaatcccat acctgtagat tctccaaacc ttcggccgtt ttcacctgta taaaagggtt
     2881 caaagacccg ccggatatct tttttaggga ttccgacacc ctggtctttt acctccaaaa
     2941 ccttgccttc ttcgctatca caagccgaaa caataacttt tttgttttca ccctcagtgt
     3001 acttaatagc attcaccaga agctgttcta ctatgaactt tagccatttc ttgtctgact
     3061 taaccgtcac attcgagtct atcctaactt ccggatacac ccttttgcga ataaaatacc
     3121 ttttttgctc atttataact tcagttacaa tggaatgcaa taagactgtt tcaacggcaa
     3181 aatcatgttc aaaacttgta agcctcgcca tattcagagc catgttcaac cccctgctga
     3241 tgcgttccaa ctcttgccgg actttttcta cataaggttc cccttcgttt tcctgtaata
     3301 ttagg
//
LOCUS       DAONUO010000239         3293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20045_length_3293_cov_2.859172, whole genome shotgun sequence.
ACCESSION   DAONUO010000239 DAONUO010000000
VERSION     DAONUO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20045_length_3293_cov_2.859172"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PK768_08410"
     CDS             complement(<1..665)
                     /locus_tag="PK768_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HQA61033.1"
                     /translation="MKNIILKSESISDEQVKSALEESLSGLKKELKKVLLLPPDFTRF
                     HSQAGKITAMYYDILKDSCQVDIMPAVGTHMAMSDEEIDKMFGEEIPKERFIVHDWRN
                     DIVKIGEVPGSYINEISEGLLDFTIDVEVNHRIVNSEYDLIISIGQVVPHEVVGMANY
                     SKNIFVGCGGKSMIDSSHFLGAVYGLERLMGKDHSPVRKVYDYAEENFLKDVPLMYVF
                     TVIS"
     gene            complement(662..2119)
                     /locus_tag="PK768_08415"
     CDS             complement(662..2119)
                     /locus_tag="PK768_08415"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HQA61034.1"
                     /translation="MNILALETSTSASKAAIYSSEEGLIKVLSIPYVEGISDTITQDA
                     KGVLNLLINEIKEILKSDTYEIDAISLSGIWHSLLLLDKDREPLTPIYTWADTRPAKT
                     VAEYRRDEELWNWFYNKTGCAIHSLYPLWKVIHLKKEEPALFKKACYITSQLQYIFEQ
                     LTGERVESKTIASGTGMFNIHSLDWDEDLLKFAGISPSMLCELREATFYAPLKEDMAQ
                     LLGLKSGIPVIIGGADGALNQVGAGALKNGIMTLSVGTSGAIRLAYDEPVLPVNRSTW
                     CYYSLNEKRLAGAATSGAGNCLEWFKKRVYKGLGLGYKELDEMADKANIQTAPIFLPF
                     LYGERNPGWQDSRQASFMGIKGHHEIGDLFHGVLEGVLFNLYQCYKALTEITGEPKEI
                     RISGGIENSPHWLQMAADIFNRDIITSGIQHMSTVGAAVLALNALGEITDIEKYEPGS
                     GQKYTPNKDKSEIYQDRYREYLYYYQMTRQEEVTK"
     gene            complement(2179..>3293)
                     /locus_tag="PK768_08420"
     CDS             complement(2179..>3293)
                     /locus_tag="PK768_08420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HQA61035.1"
                     /translation="HQNPKTLMGIITYLCYKRYQAGKYPVALVSLDNCSQNGSKLYSA
                     IKTFADTWVKNGLVDEGFSDYIADPKLVSFPWTMIDKITPRPSEQVKKVLEEDGIEDA
                     EIVKTSKNTYVSSFVNAEETQYLVIEDAFPNSRPPLEKAGIIFTDRQTVDKVEKMKVG
                     TCLNPLHTILAIYGCLLGHTSIAEEMKDIQLKAFIEKTAYEEGLPVVVDPGIIKPEDF
                     IYEVINQRFPNPFVPDTPQRIASDTSQKIPVRFGETLKAYIATGKRDISTLKYIPLFF
                     AGWLRYLMGIDDEGKPFVPSPDPMLEKLRGYVKDIKLGDKGPFTEALRPILSDSSIFG
                     VNLYEYNLASKVEAFFEELVAGKGAVRRTLEKYITP"
BASE COUNT          751 a          799 c          701 g         1042 t
ORIGIN      
        1 gatataacgg tgaaaacata cattaaggga acgtccttta aaaagttttc ctctgcatag
       61 tcgtacactt tccgaacggg agaatggtcc ttgcccatca atctttccaa accatagacg
      121 gctcctaaaa agtgcgagct gtctatcata ctcttgccgc cgcatcccac aaagatgttt
      181 ttactgtagt tcgccatacc cactacctcg tgggggacca cctggccgat ggaaatgatt
      241 agatcgtatt cggagtttac aattctatgg tttacttcaa catctatcgt aaaatcaaga
      301 agaccctcgg aaatttcatt gatatagctt cctggtactt cgcctatttt aactatgtca
      361 tttctccaat catgtactat gaatctttct ttcggtatct cttcgccgaa catcttgtct
      421 atttcctcat cactcattgc catgtgcgtg cccactgcag gcatgatatc tacctggcaa
      481 ctgtctttca agatgtcata gtacatggcc gttatttttc ccgcctgcga gtgaaacctt
      541 gtaaaatcag ggggtagaag cagtactttt ttcagttctt ttttcagtcc agacaggctc
      601 tcttctaacg cactttttac ttgctcatca ctgatgcttt cagattttaa gattatgttt
      661 ttcattttgt cacctcttct tgccgggtca tttggtaata gtacaaatac tcccggtacc
      721 tatcctggta tatttcactc ttgtccttgt ttggagtata cttttggccg gaccccggct
      781 catatttttc aatgtcagta atctctccca gggcatttaa tgccaaaaca gcagctccta
      841 cggtggacat gtgctgaatg cccgaggtga ttatatccct gttgaagata tccgccgcca
      901 tttgcagcca atggggagaa ttttctatgc cgcctgaaat tcgtatttcc ttcggctcac
      961 cggttatctc ggttaaagct ttgtagcatt gatagaggtt gaataggacc ccttccagta
     1021 ccccgtgaaa tagatcgccg atttcatgat gaccttttat tcccataaag gaagcttgcc
     1081 tgctgtcctg ccagccgggg tttctttccc cgtacaggaa gggcagaaaa atgggtgcag
     1141 tttggatatt tgccttatct gccatttcat ctagctcttt gtagccaagg cccaagccct
     1201 tgtatacccg tttcttaaac cattccaaac aattgcctgc tcctgatgtg gcggcaccag
     1261 caaggcgttt ctcgttaagg ctatagtagc accaagtcga ccgatttaca ggcagtaccg
     1321 gttcgtcgta agctaagcga atcgctccgc tagtccctac cgataaggtc atgatgccat
     1381 tttttaacgc gccggcacca acctgattca aggctccgtc tgctcctcca attattaccg
     1441 gtattccgct ttttaagcca agaagctgtg ccatatcctc tttcagcggc gcataaaatg
     1501 ttgcctctct tagctcgcac agcatggaag ggctgatgcc tgcaaatttt aacaggtctt
     1561 cgtcccaatc caagctgtgt atattgaaca ttcccgtccc cgaggcaatt gtcttggatt
     1621 caaccctttc acccgtcagc tgctcgaaga tgtactgtaa ttgtgatgtg atatagcatg
     1681 cctttttaaa taaagccggt tcttcttttt ttagatgtat gaccttccac agggggtaca
     1741 ggctgtgaat ggcacagcct gttttgttgt aaaaccagtt ccaaagctct tcatcgcgac
     1801 ggtactcggc caccgttttg gcagggcggg tatccgccca ggtgtatata ggagtcaagg
     1861 gttccctgtc cttgtccagc agcagcagac tgtgccatat tccgcttagg ctgatggcat
     1921 caatttcata tgtgtcagat tttagtattt cttttatctc atttatcagc aggtttaata
     1981 ctcccttggc gtcctgggtt atcgtatcgc tgataccctc gacatacggg atgctcaaaa
     2041 cttttatcag cccttcttcg ctggaatata tagcagcttt tgaagcagag gtgctggttt
     2101 ccagtgctaa tatattcaaa aaacatcgct ccttaacaat ttattaatgc caatagataa
     2161 agctttggat atagactctt acggtgtgat atacttctcc agagtcctcc tcactgcccc
     2221 tttacctgcc acgagttctt caaagaatgc ctctaccttt gatgccaaat tatactcgta
     2281 aaggtttacg ccgaatatgc tgctgtccga tagtatgggc cttagcgcct ccgtaaacgg
     2341 ccccttgtct ccgagcttaa tgtcttttac gtagccccgc agtttttcca gcatcgggtc
     2401 ggggctggga acaaagggct tgccttcatc gtctattccc atgaggtatc tcagccaacc
     2461 tgcaaagaag agaggaatgt acttcaaggt agatatgtct cttttgccgg ttgcaatgta
     2521 agcttttaag gtttctccaa agcgtaccgg aatcttctgg gatgtatcgg aagctatcct
     2581 ctggggcgtg tccggtacaa agggattggg gaagcgttgg tttattactt catagatgaa
     2641 gtcttcgggt tttattattc ccgggtccac tactacaggc aggccttctt catatgctgt
     2701 tttttctatg aatgctttga gctgtatgtc tttcatttct tcggcaatgg aagtgtgccc
     2761 cagcaggcag ccatatattg caagtatggt gtgcagggga ttcaagcatg tccctacctt
     2821 cattttttct accttgtcca cggtctgtct atcggtaaaa attattccgg ccttttcaag
     2881 aggcggcctt gagttgggga aagcatcttc tatcaccaga tactgggtct cttctgcatt
     2941 cacaaaagag gatacgtacg tgtttttgga ggtttttacg atttccgcat cttctatccc
     3001 gtcttcttcc aacacctttt ttacctgttc agaaggcctt ggcgttattt tatctatcat
     3061 ggtccagggg aatgatacca gcttggggtc tgctatatag tccgagaatc cttcatctac
     3121 caggccattt tttacccaag tatcggcaaa agtctttatg gcgctgtaca gctttgaacc
     3181 gttctgggag cagttgtcaa ggcttaccag ggcaacggga tacttgccag cctggtatct
     3241 cttgtagcat aggtatgtta tgattcccat aagagtcttg gggttttggt gat
//
LOCUS       DAONUO010000240         3280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20150_length_3280_cov_3.835659, whole genome shotgun sequence.
ACCESSION   DAONUO010000240 DAONUO010000000
VERSION     DAONUO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20150_length_3280_cov_3.835659"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PK768_08425"
     CDS             complement(<1..894)
                     /locus_tag="PK768_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61036.1"
                     /translation="MTLAHWLYVAGILSVIVAMLFRRNVVIPSMIFTFLIGWNFNGSI
                     INGILTIFNASLVAGQDLFNIFLIIALMVMMLKAISITGADELMVKPLKKLMVSPFVS
                     YLVLIVSTYLISLFFWPTPAVPLIGALLVPVAVKTGLPPMLGAMALAFAGQGMALSGD
                     VIIQGAPGLTAKAAGISEALATQYGGILSIITGITAIVLGYLMTRHETNAFSIEDCQA
                     ATGDTKDKCNEVKGQQYAGVLLLILIVAMAFVVFAMSHFQLKGGDASALLGGTAALVT
                     IIASLLVHKGEGFDVICDYISD"
     gene            complement(1311..1820)
                     /locus_tag="PK768_08430"
     CDS             complement(1311..1820)
                     /locus_tag="PK768_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HQA61037.1"
                     /translation="MKRVAILIEKFFDEKELIYPYIRLLEEGYEVHLVGTEKNAVYPG
                     KSTFTEKSTHAASEVKADDYDGVVIPGGYSPDHMRRNPHMIEFVKEMDRQGKPIAAIC
                     HGPWMMASCCNLKGKKLTGFFAIKDDIVNAGAEYVDAEVVVDGNLITSRTPKDLPAFM
                     KAFIEKVGK"
     gene            complement(2191..2847)
                     /locus_tag="PK768_08435"
     CDS             complement(2191..2847)
                     /locus_tag="PK768_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQA61038.1"
                     /translation="MSKINKELAQLLVDYGKKMYREGLVTGTGGNISLRVPGENAFLI
                     SPSGMPYEEITVDDIVMMDMEGNQIAGKRRPSVEKNMHRMILAMRPEFNAVVHTHSMY
                     ATAYACLSKPLPVFLDAMSCVFGGPVRCAEYARIGSVELAENVEKAMRGTNVAFLANH
                     GCIGAGETIEQAYANVELLELCCKTYLAACSAGIPSLIREEDVRLTREEFVTKYGQKD
                     "
BASE COUNT          905 a          803 c          576 g          996 t
ORIGIN      
        1 gtcggatata tagtcgcaaa ttacatcaaa gccttctcct ttgtgaacta aaagggaagc
       61 aataattgtc accaaggctg ccgttcctcc gagaagggct gaagcgtctc cgcctttaag
      121 ctggaagtga gacatcgcga aaactacaaa agccatagcg actattagaa tcaaaagcaa
      181 gacacctgca tattgttgcc cttttacctc attgcactta tcctttgtat cccctgtagc
      241 agcctgacag tcttcgatgg agaaagcatt agtctcatgt ctcgtcatta aatatcctaa
      301 cactattgcg gttatacccg tgattataga tagaataccg ccgtactgtg tagctaaggc
      361 ttcagaaata ccggcagctt ttgccgtaag gcccggtgca ccttgaatga taacatctcc
      421 cgaaagggcc attccctggc cggcaaaggc caaggccata gctccaagca tcggaggaag
      481 accggtttta actgcaaccg gcaccagcaa tgcccctatc agcggcaccg caggggttgg
      541 ccaaaagaat agtgaaatta aatacgtaga aacaatcaat accaggtagc tgacaaaagg
      601 cgaaaccatc aatttcttca aaggctttac catgagttcg tcagcaccgg ttatactgat
      661 ggcttttagc atcattacca taagggcaat gatcaggaaa atattgaaca gatcctgacc
      721 cgccaccaag ctggcattaa agatggtcaa aatgccgttg ataatgcttc cgttaaaatt
      781 ccatcctatc aaaaaagtga agatcatgga tggaattact acgttgcggc gaaagagcat
      841 agccacaatg accgaaagta ttcccgctac atatagccaa tgagctaaag tcatgatact
      901 cctccccatt tctcctcatt tatatccatg ttttccaaaa cgttcctcat cattgatgag
      961 ttcctggaca acaattcata ttataaatga gctatttaaa taatgcaacc ggaaaaaaac
     1021 acccgacagc gcaagaaatt ccgctgtcgg gctaaaaatt aagctttttt taaacaaaaa
     1081 gccgcattat gcggcattaa gctaatcaaa acacgggctt attcttcata gataattgaa
     1141 aatatctttt ctacatcctt tttggctttg ataatttttt cctttgcaac tctatcgtca
     1201 aacaggtatt tctctaacat ttttcgcgcc tccactgaaa atgattatcg ttatctatgc
     1261 ttattataca atgataacca ttttcagtca aggggttttc aaaagttgtt ttacttgcca
     1321 actttctcaa tgaaggcttt catgaaagcc ggcaaatcct tgggggttct tgaggttatc
     1381 aggttcccat ccaccactac ttctgcatct acgtactcgg caccggcatt tactatatca
     1441 tctttaatcg caaagaatcc cgtcaacttt ttccccttca aattacagca ggatgccatc
     1501 atccacggcc catggcagat agcagctatc ggcttgccct ggcggtccat ttcctttaca
     1561 aattcaatca tatgtggatt tctcctcatg tggtccggtg aatatcctcc gggtattacg
     1621 actccatcgt agtcatctgc cttgacttca cttgctgcat gggtgctctt ttcggtgaaa
     1681 gtgctttttc cgggatatac tgcgtttttc tccgtgccta ccagatgaac ctcataccct
     1741 tcctccagca agcggatata ggggtaaatc aattcttttt catcaaagaa cttttcaatg
     1801 agtatggcca cccgtttcat ctttaaacat acctcccctg ataatatttc tctttgaata
     1861 tataatttga catactttgc ctaattcctg caatttttaa tattaattat tatattacgt
     1921 atttttgtct gtatataaaa ataaaagccg cgtcggcggc aaaataatct tgcaatcata
     1981 tatctataat ttactcaaat ttgtaatcct cttctgccct tgcaaccttt tctcttacca
     2041 gattgtcttc gaataaatac ttaaacaaca ttgttttcac ctccactgat aatgattttc
     2101 attctctggt tatattatat tatgaaaatc attatcagtc aagtgctttt ttaccttctt
     2161 ttattatttt ttataaaaaa ttttccagcc ttaatctttc tggccgtatt ttgttacaaa
     2221 ttcttccctg gtgagcctta catcctcttc tctgataagg ctgggaattc ctgccgaaca
     2281 tgcagcaagg taggtcttgc aacacagctc taggagctcc acattggcat acgcctgctc
     2341 gatagtctct cctgcgccga tgcagccgtg atttgctaaa aatgccacat tcgttccccg
     2401 catagctttc tccacatttt cggcaagttc aacggaacct attctggcgt actctgcaca
     2461 tctgactggg ccgccgaaca cgcaggacat ggcgtctaaa aagacgggaa ggggtttgct
     2521 aaggcaagca tatgccgtag cgtacatgga atgggtatgt acaaccgcat taaactccgg
     2581 cctcattgcc aaaatcattc tgtgcatgtt cttctctacc gatggacgcc ttttgccggc
     2641 gatttgattg ccctccatgt ccatcattac aatatcgtca actgtaattt cctcataggg
     2701 catgcctgat ggcgatatta aaaaggcatt ttctcccgga accctaaggc tgatattgcc
     2761 gccggtcccg gtaaccaaac cctccctgta cattttcttg ccgtagtcta ctagcagctg
     2821 ggccaactcc ttattaattt ttgacacttt tcattcctcc ttacaatcct gccaaaggga
     2881 cgtttcgttt gccatgtttc cctgtcttac attacattgg gacatattgt ttgtcatata
     2941 atacgcaggt gaagataacg gcactctcac taaatcaaga cctgttattc taaagcttta
     3001 cacttcactc cacatgaatt cacgggcaat ttggcatcct gccaagtgcc caaagcttat
     3061 cctgcacgct gcccatgctc cgcttgttcc aagcttaaga ataacaacgg tcttagattc
     3121 ttgccgttat tctcacctca tatgacaaag aggctctcag tctgacagat ttgattatca
     3181 tcaaaacaag ggaacacttc cttccccaaa gtatcccccg atactttata ataattatat
     3241 gaaacttttt tatctttttc acgtctaaag taatgtaacg
//
LOCUS       DAONUO010000241         3243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20419_length_3243_cov_4.488394, whole genome shotgun sequence.
ACCESSION   DAONUO010000241 DAONUO010000000
VERSION     DAONUO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20419_length_3243_cov_4.488394"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..180)
                     /locus_tag="PK768_08440"
     tRNA            complement(105..180)
                     /locus_tag="PK768_08440"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Ala,seq:cgc)
     gene            complement(302..616)
                     /locus_tag="PK768_08445"
     CDS             complement(302..616)
                     /locus_tag="PK768_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA61039.1"
                     /translation="MPFKKVNEKEEINRRLENDAELKKAYDYAQQEYEVAKQLKKTRN
                     EMGFSQREVARKSGLTQQMVSRIETLDNSPTLRNLIRYADSVGLQIKLEKKNKRMRYI
                     KV"
     gene            complement(639..986)
                     /locus_tag="PK768_08450"
     CDS             complement(639..986)
                     /locus_tag="PK768_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQA61040.1"
                     /translation="MNVHHYTTSGGKDLILDYIDRLPKNEKAAGLTILDKLENEGLVA
                     LDVLNTRQLRGKLWEIKFYDDNRIMYIVADMDNIYLIHACKKQKGKAEKFELDKAIKR
                     VKELESILGKRFV"
     gene            complement(1143..>3243)
                     /locus_tag="PK768_08455"
     CDS             complement(1143..>3243)
                     /locus_tag="PK768_08455"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HQA61041.1"
                     /translation="EEFEKRLTFFDTEITSENVEDALFYLSRIDALNIEGGFLVTYKA
                     LTIERIEKDNKKRYTIEDYKKLDEFYKNKTQQIHIVGEYARKMLEDYKAALQFVDDYF
                     QLNYNSFLRKYFKGSRQEDISRNITPTKFRQLFGELSPTQLKIINDNESKYIVVAAGP
                     GSGKTRILVHKLASLLLMEDVKHEQLLMITFSRAAATEFKKRLIKLIGNAANFIEIKT
                     FHSYCFDLLGRVGTIEKSDEVIKEAIARIKSGEVEIGRITKTVLVIDEAQDMDANEFE
                     LISTLMEQNEEMRVIAVGDDDQNIYEFRGASSEYLNKLIVEQEAVMYEMVENYRSKSN
                     LVDFTNQFAKRIPHRLKYTPIIAVQNDNGRIKLVRYRSGNLIVPLVNDILSEELTGTT
                     CVLTKTNEEAVQVTGLLTKNGMHARLIQSNEGFNLYNLLEVRYFLSQLNLTEGIYTIS
                     DDAWANAKRRLKERFGSSLNLELCFKIIKDFEATNPKTKYKSDLEIFIRESKLEDFFE
                     QDIDTIFVSTIHKAKGREFDNVFLMLDQFNPGTDEAMRQLYVAMTRAKRNLFIHYNGN
                     YFDSIRTQGLEIVNDNGVYHPPRQLAVQLTHKDIWLDSLISCQKQISRLNSEDLLIVD
                     ENCCRNSMGQPVLKFSKQFIGQIETMGQKNYKPKTARVRFIVYWLKEDSEKEIKIVLP
                     EVYFEKENNSENNTKNR"
BASE COUNT          830 a          735 c          533 g         1145 t
ORIGIN      
        1 taccaagccc atagcggact ttcaccgcct agttactgcc catgtcgggc gaacacaaaa
       61 gaagccggct gcatttgcaa tcggcctctt ttaataaaat gttgtggtgg agataggggg
      121 attcgaaccc ccgaccttct gcatgcgaag cagacgcgct cccagctgcg ctataccccc
      181 acggattttt tatttgtttc actttgatat tataaaacgg ccctttgcaa ttgtcaaaca
      241 caacaaacat ccctgcgtat actctccaat ggatgtttgg agatatatta gaatattgta
      301 atcacacctt aatatatctc atccttttgt ttttcttctc cagtttaatt tgtaatccca
      361 cgctgtctgc atatcttatt aaatttctta gtgtaggaga attatccaaa gtctcgatcc
      421 tggaaaccat ctgctgagtt agcccggact tccttgcaac ttctctctgt gaaaacccca
      481 tttcatttct agtttttttg agttgctttg caacttcata ctcttgctgt gcataatcat
      541 aggccttctt aagctcagca tcgttttcca atctcctgtt tatctcttct ttttcattaa
      601 cttttttgaa tggcataaaa tcacctcata ttttttactt aaacaaatct ttttccaagt
      661 atactttcta attctttaac tctttttatg gctttatcca attcaaactt ttcagctttg
      721 cctttttgct ttttgcaagc atgaatcagg tatatattat ccatgtctgc tacaatatac
      781 ataatccgat tatcgtcata aaacttgatt tcccaaagct tacccctaag ctgtcttgta
      841 tttagtacat caagtgccac tagcccttcg ttttctagtt tatcaagaat ggtcaatcca
      901 gctgcttttt catttttcgg aagcctgtca atatagtcaa gtattaagtc tttgcctccg
      961 cttgttgtat aatgatgtac attcatatta tttctcctgt actttatgct catacatcat
     1021 atttgatgta tgagcaatac tattttacat catatttgat gtatgagcaa catatatttc
     1081 aatttgctta cgcctatcct gcaatgttca ttaaattgct gtacctgctt cttccaaatc
     1141 tattaccggt tttttgtatt attctctgag ttgttttctt tctcaaaata aacctcgggt
     1201 aatacaatct taatttcttt ctcagaatcc tccttaagcc agtatacaat aaatcttact
     1261 ctcgctgttt taggcttata gtttttttgc cccattgtct caatctggcc aataaactgt
     1321 tttgagaact tgagtaccgg ttgtcccatt gaattgcggc agcagttttc atcaacgata
     1381 agtaaatctt cgctgttaag ccgtgaaatt tgtttttgac aggaaatgag ggagtccagc
     1441 catatgtcct tatgagttaa ctgcaccgcc agctgacggg gaggatgata aaccccgtta
     1501 tcgttaacta tttcaagccc ctgagttctt atagaatcga aataattacc gttatagtga
     1561 atgaataggt tccgttttgc ccttgtcatc gcaacgtaca gctgtcgcat ggcttcgtcc
     1621 gttcccgggt tgaattggtc aagcattaga aaaacgttgt caaactcccg tccctttgcc
     1681 ttgtgaatgg ttgaaacaaa tatggtatca atatcttgtt caaaaaaatc ctccagcttg
     1741 gattcacgaa taaagatttc caagtcagat ttatatttgg tctttggatt tgtcgcttca
     1801 aaatccttaa taattttaaa gcataactcc aggtttaagc tgctgccaaa cctttctttc
     1861 aatctacgtt ttgcatttgc ccaggcgtca tcgctaatgg tatagatgcc ctcggttagg
     1921 tttagctgac ttagaaaata ccttacttcc agaaggttgt atagattaaa cccttcattc
     1981 gactggatta gtctcgcgtg catgccattc tttgtaagca atccggtaac ctgtaccgct
     2041 tcttcattgg tcttggttag tacgcaggta gtacccgtaa gctcttctga taatatatcg
     2101 ttcacaagcg gtacaattag gttgccgctc ctgtaacgga ccaacttaat tcttccgtta
     2161 tcattctgta cagcgataat tggagtatac tttaatctat gcggaatcct ctttgcaaac
     2221 tggttggtaa agtcaacaag attacttttg ctccggtagt tctcaaccat ttcatacata
     2281 acagcttctt gctcaacaat cagcttgtta aggtattctg aacttgctcc tctgaattca
     2341 taaatattct ggtcatcatc cccaacggca attacgcgca tttcctcatt ctgttccatc
     2401 aaagtgctga ttagctcaaa ttcgttcgca tccatatcct gggcttcgtc aatcactaaa
     2461 acagtttttg tgatcctgcc tatttcaact tccccacttt taatccttgc tatggcttcc
     2521 tttattactt cgtccgactt ttctatggtg ccaacccttc ccaataagtc aaaacaatag
     2581 gaatgaaacg tctttatctc tatgaaattc gccgcattgc caataagctt tattaaacgc
     2641 ttcttaaact cggtagccgc cgccctggaa aaagtaatca ttaataactg ttcatgtttt
     2701 acatcctcca tcagcagcag tgatgcaagc ttgtggacca aaatccgggt ttttccgctt
     2761 ccgggaccgg ctgctactac tatatacttg gattcgttgt cgtttatgat ttttaactgg
     2821 gttggtgata attcaccaaa aagctgcctg aacttggtgg gagtaatgtt cctactaatg
     2881 tcttcctgac ggctgccttt aaagtatttc ctcaggaagg aattataatt cagctgaaaa
     2941 taatcgtcca caaactgcag ggctgccttg tagtcttcca gcatctttct ggcgtattcg
     3001 cctacaatat ggatctgctg tgtcttgttc ttgtaaaact cgtccagttt tttataatcc
     3061 tcgatcgtat accgtttctt gttgtccttt tcgattcgtt ctatggtcag cgccttatat
     3121 gtaactaaaa aaccaccttc tatatttaat gcatcaattc tggacaggta aaaaagagcg
     3181 tcttccacat tttcagaagt tatttctgta tcaaaaaacg tcaacctttt ttcaaactcc
     3241 tct
//
LOCUS       DAONUO010000242         3230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20526_length_3230_cov_3.684724, whole genome shotgun sequence.
ACCESSION   DAONUO010000242 DAONUO010000000
VERSION     DAONUO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20526_length_3230_cov_3.684724"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..518)
                     /locus_tag="PK768_08460"
     CDS             complement(18..518)
                     /locus_tag="PK768_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HQA61042.1"
                     /translation="MKELNLAAVSEDVINQLPKGALLTVKANDKLNTMTIGWATVGRI
                     WNKPIMTVLVRFSRHTHEVISRADSFTVSFSKDGSLKSAFATCGKKSGRDTDKFQECG
                     LTPVYIDNIESPYIAEGDLHFICKIVYKNTLEPELLSEEIKDIWYKDNDYHCIYFGEI
                     VKILSK"
     gene            614..877
                     /locus_tag="PK768_08465"
     CDS             614..877
                     /locus_tag="PK768_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61043.1"
                     /translation="MTDAQLKTVFTLLNYTIGLFITVISVKLYARKGLLAPLYITTAI
                     IVAGPIENMLMQMVSREDRWIIDQITSVIFLFFLLLAVLELSK"
     gene            1050..1913
                     /gene="speE"
                     /locus_tag="PK768_08470"
     CDS             1050..1913
                     /gene="speE"
                     /locus_tag="PK768_08470"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HQA61044.1"
                     /translation="MMEMGKYLKRVGDDLWFTETDRDNLKIDYRIKDIVFSETSPFQH
                     IMILDSYDFGRMLVLDDAIQTTAKEGYIYNEMITHIPLNIHPNPKNILIIGGGDCGAA
                     REVAKYPNVERIDMVEIDEMVVQASKAHLKEVSGDLSDPRVNFIFDDGASYVKQKRDY
                     LYDVVIIDSSDPVGPALKLFELEFYTDVYNALAEDGLMACQSESPVFYSEMMVNINSR
                     LRSLYPIVKLYLATVPTYPGGFWSFTIGSKKYKDTNPDAFDKETKYVNKDILESCFAL
                     PNFVKERLEID"
     gene            complement(2081..2650)
                     /locus_tag="PK768_08475"
     CDS             complement(2081..2650)
                     /locus_tag="PK768_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HQA61045.1"
                     /translation="MALKTKDMILIALFAALTAVGAFIKIPTPIVPFTLQYLFCAYSG
                     ILLGARRGFYSQLLYLSIGLMGFPIFTNGGGIMYIFQPTFGYIVGFLLCSYIIGKLTE
                     RLTNITVTGLFSSIVAGMLALYFCGIAHLYLIMNFYLGKAMTVKAAIITGTFPFILTD
                     LLYSLLIAVTAAAIVPLLKKLGLVGDLSS"
     gene            complement(2908..>3230)
                     /locus_tag="PK768_08480"
     CDS             complement(2908..>3230)
                     /locus_tag="PK768_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQA61046.1"
                     /translation="QVLEREGNNILRKINPTSYVIPLCIEGRSLSSEEIAKKLSDLML
                     NGRSHITFIIGGSLGLSDAVKARGDFHLSFSRLTFPHQLMRLILMEQIYRCFKIIRGE
                     TYHK"
BASE COUNT          995 a          636 c          606 g          993 t
ORIGIN      
        1 atttcaaagt caaatcttta ctttgataag atctttacaa tctctccaaa ataaatacaa
       61 tggtagtcat tatccttgta ccatatatct tttatttctt cagaaagcag ctctggctcc
      121 aaagtgttct tatagactat tttacatata aagtgcagat ctccttctgc aatataaggc
      181 gactcaatat tgtcaatata taccggcgtc aggccgcact cctggaattt atcggtgtcc
      241 cttcctgatt ttttgccgca tgtcgcaaaa gcacttttaa gggacccatc tttagaaaag
      301 cttacagtaa acgagtcggc ccttgaaatt acctcatggg tatggcggga aaatctaacc
      361 aggacggtca ttatgggctt gttccagata cggccaacgg tagcccatcc aatggtcatt
      421 gtgtttagtt tgtcattggc ttttacagtg agtagcgctc cttttggcag ttgatttatg
      481 acatcttccg atacggctgc aaggtttagc tctttcataa aaatctctcc ttctctattt
      541 atttttattc ttacataacc aaatgagcta tggcttaata tattaataca aaggataaaa
      601 ttgaggtgtt agcatgacag atgcgcagct aaaaactgtt tttaccctgc tcaattatac
      661 tataggcctt ttcatcacgg ttatatcagt taagctttat gccaggaaag gacttttagc
      721 acccctttac atcactacag caataatcgt ggcaggccca attgaaaata tgttgatgca
      781 aatggtaagt cgtgaggatc ggtggataat agaccagata acgagcgtta tttttttgtt
      841 ctttttactg ctggcagttt tagaattatc caagtaagca caatcaccta aattatattt
      901 tgccattaat taacccttaa atcaacttta tttatatata tgatgacgta gactttcgat
      961 ttcatcgtaa attcccgttt ttgattaatt atatgattca tgttattatg ttaagtagct
     1021 aaaactaaat gatgtaagga ggttttgcaa tgatggaaat gggaaaatac ttgaaaagag
     1081 taggtgacga tctttggttc actgaaactg atcgggacaa tcttaagata gattatcgaa
     1141 taaaagatat agttttctca gaaacctccc catttcagca catcatgatc ctggattcat
     1201 atgattttgg ccgaatgctc gttttggacg atgccattca aactactgct aaagaaggat
     1261 acatatataa tgaaatgatt acacacatac ccctcaatat acatccaaat ccaaaaaata
     1321 tcctcattat cggcggcggc gactgcggtg ctgcgaggga agtagccaaa tatcccaatg
     1381 ttgaaagaat agatatggtt gaaattgatg aaatggtagt ccaagccagt aaggcccacc
     1441 ttaaagaagt ttccggggat ctatcggatc ctagggtaaa cttcattttc gatgatggtg
     1501 cctcatatgt gaagcaaaaa agggattatt tgtatgacgt ggttataatt gattcctcgg
     1561 atcctgtggg accggcatta aagctatttg agctggagtt ttatacggat gtgtacaatg
     1621 ctttagctga agacggcttg atggcttgcc agagcgaatc cccggtcttc tattctgaaa
     1681 tgatggtaaa tataaacagt cgattaagga gcctatatcc aatcgtcaaa ttatacttgg
     1741 caaccgtacc tacttatccc ggaggatttt ggtcatttac aataggctca aaaaaatata
     1801 aagatacaaa ccccgatgcc tttgataaag aaaccaagta tgtaaacaaa gatattttag
     1861 aaagttgctt tgcccttcct aacttcgtaa aagaacgatt ggaaatagat tgacctgaaa
     1921 atggcactga taggatgttt atcagtgcca tttatttccc atagcaagta ctctttcgga
     1981 tagcttgtcc cgttcttgac atagcaataa aaactgctaa atcccatgct tagtaaaagc
     2041 tctcccatga atttttgtac taatagttct tattttttat ttagctgcta agatccccta
     2101 caagccccag cttctttaat aaaggcacaa ttgcagccgc cgttaccgct atgagtaagc
     2161 tataaagcaa atccgttagg ataaagggaa aggtcccggt gattatagca gcttttacgg
     2221 tcatggcttt acccaaataa aaattcataa taaggtataa gtgcgcgatg ccgcaaaagt
     2281 acagggcaag catgcctgcc acaatcgagc tgaacagacc cgttaccgta atatttgtga
     2341 gtctttcagt aagcttgcct attatgtatg aacacagcaa aaagcccact atataaccga
     2401 aagtcggctg gaaaatatac atgatgcctc cgccattcgt aaaaatcgga aaacccatca
     2461 acccaatgct caagtacaac aattgagagt aaaagcccct tcttgcacca agcaatatgc
     2521 ccgaataggc acaaaacaag tactgcagtg taaaaggaac aataggtgtg ggtatcttaa
     2581 taaatgcacc cacggcagtc agcgctgcaa atagagctat taagatcata tcctttgttt
     2641 taagtgccat atacaaaact cccatcaata ccatgtttcc tacattttaa caggatatta
     2701 cggacattgt caaccatttt gttttataga gtgtacatat gacgctcttt tatcttccgt
     2761 gattctagga caaaatcata tattttatgg ctcttccaaa gttcatgatg attgcatttc
     2821 tttcaaccaa aaagttgttc tttctcataa aatcaccacc taaagtcact atttatcatc
     2881 gtatcgttta tctatctgca aggtatatta tttatgataa gtttcgcccc tgattatctt
     2941 aaagcagcgg tatatctgtt ccattagtat taagcgcatg agttggtgag ggaatgtaag
     3001 tcttgaaaaa gaaagatgga aatcccctct tgccttaact gcatcggaaa ggccaaggga
     3061 tccgcctatg ataaaggtaa tatgagatct gccatttagc atcaggtcgc tcaacttttt
     3121 tgcaatttct tctgaggaaa gggagcggcc ttctatacac agtggaatga cataggacgt
     3181 gggatttatc tttcgaagta tgttattgcc ttcccgctcc agtacctgtt
//
LOCUS       DAONUO010000243         3218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20607_length_3218_cov_4.206450, whole genome shotgun sequence.
ACCESSION   DAONUO010000243 DAONUO010000000
VERSION     DAONUO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20607_length_3218_cov_4.206450"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK768_08485"
     CDS             <1..234
                     /locus_tag="PK768_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HQA61047.1"
                     /translation="AIEKLAQDGYVFDIIFLDPPYYKNHIEKTLKALKDFSIAGSIVA
                     VQHPKDESFSFSGFSFLKDKKYGRNALTFLVKE"
     gene            240..722
                     /gene="coaD"
                     /locus_tag="PK768_08490"
     CDS             240..722
                     /gene="coaD"
                     /locus_tag="PK768_08490"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQA61048.1"
                     /translation="MDIGIYPGSFDPITNGHLDIITRSAKLFDKLIVAVLSNPRKTAL
                     FTVDERIEMIRESVKDIDNIEIDSFSGLLIDFARLKKAKVIVKGLRAVSDFEYELQMA
                     LMNNKLDSEIETIFIMTSSKYSFLSSSIIKEVSSFGGCVRGLVPPIVEKRLKQKYHNL
                     "
     gene            748..1194
                     /locus_tag="PK768_08495"
     CDS             748..1194
                     /locus_tag="PK768_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQA61049.1"
                     /translation="MKCFKIIEELQSIIINGSKIPLSNKVVISQDQVLNLLDELIREI
                     PEDIKDAEKILQDRQKILIDAQQQGELIIKEAQATIEKMVDQEEITKLARDKSDQILA
                     FAKQTARELRAGANAYADEILEDAQKHLEKLLETLKQSREELKQSR"
     gene            complement(1196..2455)
                     /gene="ylbJ"
                     /locus_tag="PK768_08500"
     CDS             complement(1196..2455)
                     /gene="ylbJ"
                     /locus_tag="PK768_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HQA61050.1"
                     /translation="MKTKIPHHKISKAYQLILPAIAVIITISIIQYPDEAFNAALNGL
                     DVWFTIVLPALLPFFIGSQLLMGLGVVHFMGVLLEPFMRPLFNVPGAGSFVMAMGLAS
                     GYPVGAMLTGKLVKRKLCNIWEAERLMSFTNTADPLFMVGAVAVGMLNDVRLGSIIAL
                     AHYISTLILGLIMRFYATNKETTQTLSNSGSQQGIILKATRELLRARREDNRTFGRLL
                     GDCIKDSVNSLLLILGFIILFSVVIRIVTVAGLIKMLIPFIEAIFNIFGFDRSLAPAV
                     LSGFFETTIGCQLASNADAPLMQRVIMISSIIAWSGLSVHFQVMSMISDTEIKMTPYV
                     IARFLHAILAGFVCYVMLKMPVGSFETLVVPAFAMSPQGTAESWLYVFKISTQVFVLL
                     FILIVFAAVIVNLVKSLNIIGFKVVKK"
     gene            2575..>3218
                     /locus_tag="PK768_08505"
     CDS             2575..>3218
                     /locus_tag="PK768_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQA61051.1"
                     /translation="MRNFREKSLQLLALMFIFLFVLNFYLGQNYTIIAPGVTVDLKDI
                     VTVERGTKDEGSFFLTTVTTRTLNVPLLIYAAVDPHVKIQRKEQVIPPGWTMKEYMDY
                     MEKWMQESQKIAEYVALKKAGYNPQILGDGAQIVGIMPNSPSKGILFPGDIIRKVDNE
                     PVSIADEVIEKVANRNIGEVVELEVEREDNTLTLSLPTIESQTEPGKAIVGIYIT"
BASE COUNT         1092 a          579 c          647 g          900 t
ORIGIN      
        1 gcaatagaaa aattggcaca agatggctat gtctttgaca taatattttt agatccccct
       61 tattacaaaa atcatataga aaaaacctta aaggctttga aagacttcag tattgctggt
      121 tcaatagtag ctgtccaaca cccgaaagac gagtcatttt catttagcgg ttttagcttt
      181 ttaaaggaca aaaaatatgg ccgaaatgca ttaacttttc ttgtaaagga gtgactgaaa
      241 tggatattgg tatttatccc ggaagttttg atcctattac taatggccat ttagacatca
      301 ttacaagaag tgcaaagcta tttgataagc ttattgttgc tgttttatca aatccacgaa
      361 aaacggctct gtttactgta gatgagcgaa tagaaatgat aagggaatcg gtaaaagata
      421 tcgacaatat agagattgac agtttttcgg ggctattaat agatttcgcc aggctaaaga
      481 aggctaaagt aatagtaaag ggtttaaggg cagtctctga ctttgagtac gagcttcaaa
      541 tggcactaat gaacaacaaa ttagacagtg aaattgaaac aattttcata atgaccagca
      601 gtaaatattc gtttttaagc tcaagcatca tcaaggaagt ttcaagtttt ggaggttgtg
      661 ttaggggcct tgtcccgcct attgttgaaa agcgtttaaa gcaaaaatat cataatttat
      721 aagccaaatc tttaaggggg ttttccaatg aaatgtttta aaatcattga agagcttcag
      781 agtatcataa ttaacggctc taaaatcccg ctttctaata aggttgttat cagccaagac
      841 caggtactta atctgctcga tgagcttata agagagattc ctgaggacat aaaggatgct
      901 gaaaagattc tccaggaccg gcaaaaaata ttaatcgatg ctcagcagca aggtgaatta
      961 ataattaaag aggctcaagc gacaattgaa aagatggttg accaggaaga aattacaaaa
     1021 ttggccagag ataaatcaga tcaaatactt gcctttgcca agcagacggc tcgcgaactg
     1081 cgtgccggtg ccaatgctta tgctgacgag atattggagg atgctcaaaa acaccttgag
     1141 aagttgcttg aaaccttaaa acagagtaga gaagagctta aacaatcaag ataaattact
     1201 tcttcacaac tttaaaaccg atgatattta aactcttgac taaattgaca attactgcag
     1261 caaagacaat cagtataaaa agcagaacaa aaacctgagt agatatttta aatacatata
     1321 accagctttc agctgtaccc tgaggcgaca tggcaaaagc tggtaccact aaggtttcaa
     1381 aacttcccac aggcattttt agcattacgt aacaaacaaa tcctgcgaga atagcatgaa
     1441 ggaatctggc aataacatat ggtgtcattt ttatttcggt atcgcttatc atgctcatga
     1501 cctgaaaatg cacggataaa ccactccagg cgattatgct gctgatcata attactcgtt
     1561 gcattaatgg ggcatcggca ttgcttgcta gctggcaacc gatagttgtt tcaaaaaaac
     1621 cgcttaatac ggcaggagct aaacttctgt caaagccaaa tatattaaat atagcttcta
     1681 taaacggaat taacatcttt atcaaaccgg caactgtgac aattcttata acaactgaaa
     1741 acaatataat aaacccaagt attaatagca atgagttgac ggaatctttt atgcaatctc
     1801 ctaaaagcct tccaaatgtt ctgttgtctt ctctgcgcgc cctcaacagt tcccgtgtag
     1861 cttttagtat aatcccttgt tggctgcctg aattggacag agtttgagtt gtttctttat
     1921 ttgttgcata aaagcgcatt atcagtccca atattaaagt agagatgtaa tgagcaagag
     1981 ctataatcga ccccaatcta acatcattta gcattcctac cgcaactgct ccaaccatga
     2041 aaagagggtc cgctgtattt gtaaatgaca taaggcgttc tgcttcccaa atattgcata
     2101 gttttctttt aactagtttt cctgtgagca tagcacctac aggatatccg ctggcaaggc
     2161 ccatggccat gacaaaagag cctgcaccgg gaacgttaaa caaaggtcgc ataaagggct
     2221 ccaacagtac gcccatgaag tgaactacac caagtcccat tagtagttgg gaaccgataa
     2281 aaaagggaag cagtgcgggt agtacaatag taaaccatac atcaagtcca tttagtgcgg
     2341 cattgaacgc ttcatctgga tactgaataa tagaaatagt aataataact gctatggcag
     2401 gtaaaatcaa ttgatatgct tttgaaattt tatgatgtgg aatttttgtt ttcattttcg
     2461 gcctcctaga gtattttttg gatgctaata taatatatat tgctaggatg gctttattat
     2521 tctagtcatc atagtgttct caactttgta attacgaaag aggagacaaa aaagatgcgc
     2581 aatttcagag aaaaatcttt acaattactc gcattaatgt ttattttttt gtttgtcttg
     2641 aacttttatc ttggccagaa ctatacaata attgccccag gtgtaacggt agacttaaaa
     2701 gacatagtaa cggttgaacg cggaacaaaa gatgaaggtt cttttttttt gacaactgtg
     2761 acaactcgaa ctcttaatgt tccgttgtta atatatgccg ctgtagaccc tcatgtaaaa
     2821 atacagcgaa aggaacaggt gataccaccg ggctggacta tgaaagagta catggattac
     2881 atggaaaaat ggatgcagga gagccaaaaa attgccgaat atgttgcctt aaaaaaggca
     2941 ggctacaacc ctcaaatcct cggagacggg gcacagatag ttgggattat gccgaacagt
     3001 ccttcaaagg gaatactatt tccgggtgat ataataagaa aggtggacaa tgagccggta
     3061 agtatcgcag atgaagtgat tgaaaaggtg gcaaatcgga atataggtga agtggtggaa
     3121 ctggaggtag aaagagaaga caatacccta actctttcct tacccactat agagagccaa
     3181 actgagccgg gcaaagccat agtaggaatt tacatcac
//
LOCUS       DAONUO010000244         3211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20652_length_3211_cov_3.263942, whole genome shotgun sequence.
ACCESSION   DAONUO010000244 DAONUO010000000
VERSION     DAONUO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20652_length_3211_cov_3.263942"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK768_08510"
     CDS             <1..486
                     /locus_tag="PK768_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA61052.1"
                     /translation="TGIFYTALIALFIYYLMEKTSAGYDFKMIGSNPIFARYGGINAE
                     RNMVIAMIISGALCGIAGAFEVLGNHYRFLQAISPGYAFDGMLVSLIVKNNVLGVILM
                     SIFFGMLKTGSIAMEYETAIPSELVLVIQSIIILFIAGEKGFANILRRIYAKEGAGQD
                     V"
     gene            479..1447
                     /locus_tag="PK768_08515"
     CDS             479..1447
                     /locus_tag="PK768_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA61053.1"
                     /translation="MYREIFNITLLQNTIRTATPLILAALGGLLTSHTGILNIGMEGM
                     ILLGAFFGVAGSYFFESYLMGILFAVASGIIIGLIFALFVIRLKSDEFIVGIAINMFA
                     GGLTMFLLRSIFGVKGAFSSPRIQPLPDISISFLENIPVLNVLLNDHSLFVYVSWILV
                     IAIYIFLYKTPYGLWFRAAGEFPEALDTVGISSNRIKFISSILCGIFCGFAGVHLSLG
                     YLTLFTENMSANRGFIALAAIIFGAANPLMTYVAALIFGFFDALGIRLQGVGIPSQFT
                     QMFPYIVTVLVLILVVKRERDKKRAHQARQKGLDKSQIKAADNLAK"
     gene            1460..2755
                     /locus_tag="PK768_08520"
     CDS             1460..2755
                     /locus_tag="PK768_08520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA61054.1"
                     /translation="MKDVLVLGCGCAGYYTALLCASKGLDVALVEKEALGGTGFRFGA
                     LPVKMLLDGIKDDPHMSFDELLEDYRGKMAELQKKLESDLKESGVSIVFGEGHFVGKD
                     AFQVQDKVIKAKNIVIATGSRPVNISKVPYGNRILTHKEALAMERLPKDMLILGANVE
                     GIEFATIFAHFGVKVTLVDMVDEILQGNDEDLRQPVIDGLKSLNVDFVTGIKVSEVDE
                     SQDGAVVMLETGEKLKAEKCLVCLSRLPNIPKGAADVGIEYDEVGIKVDHNFQTGVKN
                     IYAVGDVNGMCLMGSAAINQGIAAALHILGRKDSLEDISYEGIPRAIFTIPEIAGAGL
                     QEWELKEKGIKYKATRIDLAKTWRGISRRYKDGFVKVLTDKENKLQGIWMVGRDVSEL
                     VGMHGILLKEELCAHKILSSLFVHPSLSEALLDASLRLI"
     gene            2949..>3211
                     /locus_tag="PK768_08525"
     CDS             2949..>3211
                     /locus_tag="PK768_08525"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="catalyzes the reversible phosphorylytic cleavage of
                     uridine and deoxyuridine to uracil and ribose- or
                     deoxyribose-1-phosphate; involved in the pyrimidine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HQA61055.1"
                     /translation="MLQPHILCKKGDIAENVILPGDPQRVLRAAEFLDGARELAFNRE
                     FRTVTGKYKGVPVTITSTGIGGASCAIAVEELIACGGRNFLRIG"
BASE COUNT          874 a          709 c          821 g          807 t
ORIGIN      
        1 accggcatat tttatacggc acttatagcc ctgttcatat attacctcat ggaaaagact
       61 tcggcgggct atgactttaa aatgataggc tcaaatccca tctttgccag gtacggggga
      121 atcaatgccg aaagaaacat ggtaattgct atgattatat ccggagccct gtgcggcata
      181 gccggggcct tcgaagtgtt gggcaaccac taccgatttt tgcaggcaat ttctcccggt
      241 tatgcctttg acggcatgct ggtctccctt atagtaaaaa acaatgtgct aggggtcata
      301 ctcatgtcaa ttttctttgg catgttaaag accggctcca tcgccatgga gtacgaaacg
      361 gccattccat cggaactggt gcttgtgata cagtcgatta taatcctatt tatcgccggt
      421 gaaaagggct ttgcaaatat ccttcggcgg atatacgcaa aagagggggc aggacaggat
      481 gtatagggaa atcttcaata taactctact tcagaacacc ataaggaccg ccacacccct
      541 tattttagcg gcgcttgggg gtctgctcac atcccatacg ggcatattaa acatcggcat
      601 ggaaggcatg attctgctgg gagccttttt tggcgtagca ggcagttact tttttgaaag
      661 ctacctcatg ggcatcctgt ttgccgtggc atccggaatc attatcggat taatttttgc
      721 cctcttcgtc atcagattaa aatccgacga gtttatcgtg ggcattgcca taaacatgtt
      781 tgccggtggc cttaccatgt tcctcttgag gagcatattt ggggtaaagg gagccttttc
      841 atctcctagg attcaacccc ttccggatat atccatatcc tttcttgaaa atatcccggt
      901 actcaatgtg ctcctgaacg accactccct cttcgtgtac gtgagctgga tactggtcat
      961 tgccatatac atatttctct acaagactcc atacggcctg tggttcaggg cggcagggga
     1021 gtttccggaa gccctggaca ctgtgggaat tagctcaaac aggataaagt ttatcagcag
     1081 cattttgtgc ggtatcttct gcggcttcgc aggagtgcac ctgtccctgg gctatctcac
     1141 cctctttacg gaaaacatga gcgccaatcg gggcttcata gccttggccg ccatcatatt
     1201 cggagctgcc aatcccctga tgacgtacgt ggcagcactc atattcggct ttttcgatgc
     1261 cctgggcata aggctgcagg gggtaggcat tccctcccag tttacccaga tgtttcccta
     1321 cattgttacg gtgctggtgc taatccttgt tgtaaagagg gaaagggaca agaagagagc
     1381 tcaccaggca aggcaaaaag gcctagataa aagccagata aaggctgccg ataacttggc
     1441 caaataagga ggcttggcta tgaaagatgt gctggtttta ggctgcggct gtgcaggtta
     1501 ctatacagct ctcttgtgtg cgtcaaaggg ccttgacgtg gccctggtgg aaaaggaggc
     1561 ccttggaggg accggatttc gctttggtgc cctgccggtg aaaatgctcc ttgacggcat
     1621 aaaggacgat cctcacatgt ctttcgatga gctcctagaa gattatcgcg gaaagatggc
     1681 agagcttcaa aagaagctag aatcagacct taaggaatcc ggtgtcagca tagtcttcgg
     1741 tgagggccat tttgtgggga aggacgcctt tcaagtacag gataaagtca tcaaagctaa
     1801 aaacatcgta attgccaccg gcagcaggcc ggtcaacatc tctaaagtgc cctacggcaa
     1861 ccgtatcctg acccataagg aagcccttgc catggagcgg cttccaaaag acatgctcat
     1921 tttaggggcg aatgtggaag gtatcgagtt tgccacgata tttgcccact tcggcgtaaa
     1981 agtcacccta gtggatatgg tggatgaaat cctgcagggc aacgatgagg atttaagaca
     2041 gcctgtaata gatgggttaa aatccctcaa tgtggatttt gtaacgggaa taaaggtatc
     2101 agaagttgat gaatcccaag acggggctgt ggtcatgctg gagacgggtg aaaaactaaa
     2161 ggcagaaaaa tgcctcgtct gcctgtcaag gctgccgaat atcccaaagg gtgcagccga
     2221 tgtaggaata gaatatgacg aagtggggat taaagttgac cataacttcc aaaccggggt
     2281 gaaaaatata tacgccgttg gcgatgtaaa cggcatgtgc cttatgggaa gcgcagccat
     2341 aaatcagggc atagcggctg ccctgcacat cctgggcagg aaggattccc ttgaagacat
     2401 aagctacgaa ggcattccca gggccatatt tacaatcccg gaaatagccg gggcgggcct
     2461 gcaggaatgg gaactcaagg aaaagggcat aaagtataaa gccacaagaa tcgaccttgc
     2521 caagacctgg cggggcatat caaggagata caaggatggc tttgtaaaag tgctgacgga
     2581 taaagaaaac aagctgcaag gcatatggat ggtgggaagg gatgtttcgg aactagtggg
     2641 catgcatggc attttactaa aagaagaact ttgtgcccat aagattttaa gcagcctttt
     2701 tgttcatccc agcttgtcgg aggctttact tgacgcctca ttaaggctaa tataactgct
     2761 cattaaagga attaaattga taagttttca aaatatgttt ctttgcgagt tttgcttaat
     2821 gattacaaca tgcaaccctt acaaaaaacc gaaactccta tatttcttaa agaaatattt
     2881 ttgacattgt tgttctacag caagctatta agaaatcaga acacctgaat attgaaaggg
     2941 gtataattat gcttcaacca cacatactct gcaaaaaagg cgatatagca gaaaacgtga
     3001 ttttaccggg agatccccag cgcgtcctga gagccgccga attcttggat ggggccaggg
     3061 aactggcctt taacagggag tttaggacgg tcactgggaa gtacaaaggt gtgcccgtaa
     3121 ccatcacttc cacaggtata ggcggcgcat catgtgccat agcggtggaa gagctgatag
     3181 cctgcggcgg caggaatttt ctcaggatag g
//
LOCUS       DAONUO010000245         3201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20737_length_3201_cov_3.123013, whole genome shotgun sequence.
ACCESSION   DAONUO010000245 DAONUO010000000
VERSION     DAONUO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20737_length_3201_cov_3.123013"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK768_08530"
     CDS             complement(<1..344)
                     /locus_tag="PK768_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQA61056.1"
                     /translation="MAKTYKLGLALSGGGVRGAVHLGILNVLIKNKIFPDIIAGTSAG
                     SIVGALYAAGVDLEKFLEDFKSINPICLLDPTVTGVYILLLFFYYWTKRPMVHWTLPK
                     GLFKGEKIEQFL"
     gene            complement(483..1430)
                     /locus_tag="PK768_08535"
     CDS             complement(483..1430)
                     /locus_tag="PK768_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQA61057.1"
                     /translation="MATLKELAQVNERFVGGHRLCAGCGHGISVRMVLNALENDENVV
                     VGCATGCLEVASTIYPYTAWNCSFIHNAFENAGATISGVEAAYRSLKKQGKLDDDFKF
                     VAFGGDGGTYDIGLQSLSGAMERGHNLVYVCLDNGAYMNTGIQRSSATPMGADTTTSP
                     AGKVVPGKQQPRKDLAAVMAAHNIPYVAQTAISNWQDVHKKAKKAFEVDGPAFINILS
                     PCPRGWRYNTPDIVELVKLAVETRFWPLFEVENGKWKLNKKPKKYVPIEEFLKPQGRF
                     RHLFAPGNEHLIKEIQEEVDQRWLALLERCGEKPELPPR"
     gene            complement(1442..2623)
                     /gene="porA"
                     /locus_tag="PK768_08540"
     CDS             complement(1442..2623)
                     /gene="porA"
                     /locus_tag="PK768_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HQA61058.1"
                     /translation="MKSKVALTGNEAVAQAMRQINPDVVAAYPITPSTEVVQLFSSFV
                     NNGQVDTEFVTVESEHSAMSATMGASAAGARAMTCTSSQGLAYMFEVVYVAASMRLPI
                     VFPVANRALSAPINIHCDHSDAMGIKQSGVIQLFSENAQEAYDNMIQAIRIAEHPDVL
                     LPVSVNYDGFITSHSLGVLEILDDETVREFIGTYHPQEALLNVKEQISMGNFDMYDYY
                     FEHKYQQVAAMENAKRVIEEVGKEYAKISGRSYGLIDAYKLDDADMAIVVMNSAAGSA
                     KGVCDELRAKGLKVGVLKPRAFRPFPHEAIVDALKSVKAVAVLDRAETFSTLGGPMFA
                     DVRTSLYDLKADIKVVNYVYGLGGRDLRLEDIEQVYNDLADIIKTGKVDKFVRYLGLR
                     E"
     gene            complement(2613..2981)
                     /locus_tag="PK768_08545"
     CDS             complement(2613..2981)
                     /locus_tag="PK768_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA61059.1"
                     /translation="MTAFNRNFGQGRSREGIRPDMTWKELTKAGNIYEPGNANTYETG
                     DWRTIRPVWNEERCIHCLICWRYCPDSSIISTDGKFDCFDYKHCKGCGICANVCPTHA
                     IDMISEDEIDKQKEASKDEE"
     gene            complement(2978..>3201)
                     /locus_tag="PK768_08550"
     CDS             complement(2978..>3201)
                     /locus_tag="PK768_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQA61060.1"
                     /translation="TVDANQISLDTIGRVIPNTPLMGAFIKATGLMPFDEFAKHVKGE
                     LEKKFKTKPEVIEGNVKAIERAYQEVKGE"
BASE COUNT          769 a          814 c          703 g          915 t
ORIGIN      
        1 tcaagaaact gttcgatttt ttccccttta aaaagtcctt tcggtaaggt ccagtgaacc
       61 ataggtcttt tggtccagta gtaaaaaaac aacagcaata tatacacccc ggtaactgta
      121 ggatcgagta gacatattgg atttatgctc ttaaaatcct ccaaaaattt ttccaggtcg
      181 accccggcag catacagtgc gccgactata cttcctgcac tggttccggc tatgatatcc
      241 ggaaaaattt tgttttttat aagcacattt aaaatgccaa ggtgaacggc acctctcact
      301 ccaccgccgc tcaatgccaa tccaagctta taggtctttg ccactttcca ttccccccat
      361 cccaaacagc aggccctaat attaaaatat taaaggaggg ggaatttgtt gacagttgct
      421 atgacaaaaa aaccccgtac ctaaaatagg tacgggattc tattaaagca atgccttgcg
      481 cattagcggg gaggcaattc gggtttttca ccgcagcgct caaggagggc cagccatctc
      541 tggtctactt cttcctggat ctcctttatg agatgctcat ttccgggagc aaacagatgg
      601 cggaatcttc cctgcggttt taagaattct tctatcggca cgtacttctt gggcttctta
      661 ttgagcttcc acttgccgtt ttccacttca aagagaggcc aaaagcgagt ctcaactgcc
      721 agcttcacca attctactat atcaggagtg ttgtatctcc agcccctggg acagggtgac
      781 aatatgttga taaaagctgg accatccact tcaaacgcct tcttggcctt tttatggaca
      841 tcctgccaat tgctgatggc cgtctgggcc acgtaaggta tattgtgtgc cgccattaca
      901 gcagctaaat ctttcctggg ttgctgtttt ccgggcacca ccttgccggc ggggctggtg
      961 gtagtatccg cacccatagg tgtggcactg gagcgctgga taccggtgtt catgtaagca
     1021 ccgttgtcca agcacacgta gacaaggttg tgtccccgct ccatggcccc tgaaagggac
     1081 tgaagaccta tgtcgtaagt gccaccgtca ccgccgaagg ctacgaattt aaagtcgtca
     1141 tctagtttcc cttgtttttt cagggacctg tatgctgctt caactccgct gatggtagca
     1201 cctgcgtttt caaatgcatt gtgtatgaat gaacagttcc atgcagtata tggatatatg
     1261 gtagaggcaa cctccaaaca gccagtggca cagcccacca ccacattctc gtcattttcc
     1321 agggcattta gcaccatgcg cactgaaatg ccgtgaccgc atcctgcaca gagcctatga
     1381 cctccaacaa atctctcatt gacttgggca agttctttta atgtagccat tcctgcacct
     1441 cctattctct gagacctaag tatctcacga atttgtcaac tttgcccgtt ttgatgatat
     1501 ctgccaggtc attgtagacc tgctcgatat cttccaggcg caaatctctt ccgccaagac
     1561 cgtatacgta attgactacc ttgatgtcag cttttaagtc gtatagggat gtcctcacat
     1621 ctgcaaacat ggggcctccc agtgtggaga aggtctccgc cctgtcaagg accgcgacag
     1681 ccttgacgct ctttagtgca tcaactattg cttcatgggg gaatggtctg aaagccctcg
     1741 gctttagcac gccgactttt agacctttgg ccctcagctc atcgcatact cccttggcac
     1801 ttcctgcagc ggagttcatt actactatgg ccatgtcggc atcatcgagt ttgtaagcat
     1861 cgatcaggcc gtaacttctg cccgaaatct tggcatattc cttgccaacc tcttcaatta
     1921 cccgtttggc attttccatg gctgctacct gctggtattt gtgctcaaag taatagtcgt
     1981 acatgtcgaa gttacccatg gaaatctgtt cttttacatt gagcagtgct tcctgtggat
     2041 gataggtacc tataaattcc cttacggttt catcatctaa gatttccagg acacctaaag
     2101 agtggctagt gataaagcca tcatagttga cgctgacggg taaaagcaca tctggatgct
     2161 ctgcaatgcg tatggcttga atcatattgt cataggcttc ctgggcattc tcggaaaata
     2221 gttgtatgac gcctgattgc tttattccca tggcatcact gtggtcgcag tgaatattta
     2281 tgggggcgct cagggctcta ttggctaccg ggaatacaat tggaagtctc atggacgctg
     2341 ccacgtaaac tacctcaaac atgtaagcaa ggccctggct tgaggtacaa gtcatggcac
     2401 gggctccggc agctgatgcg cccatagtag cactcatggc actgtgttca ctttcaactg
     2461 ttacaaattc agtatcgact tggccgttgt ttacaaagga tgaaaacaac tggacaactt
     2521 ccgtagaagg agtaatggga tatgctgcaa cgacatccgg gtttatctgc ctcatagcct
     2581 gggcaactgc ttcatttccg gtaagggcaa ctttactctt catctttgct cgcctccttc
     2641 tgtttatcta tttcatcttc tgaaatcatg tcaatggcat gagttgggca tacattggcg
     2701 caaatgccgc aacctttgca gtgcttgtag tcgaaacagt caaatttgcc atcggtggaa
     2761 attatggaag agtcagggca gtagcgccag cagataaggc agtgtatgca gcgctcttcg
     2821 ttccagacag gcctgatagt acgccaatcg cctgtttcat aagtatttgc gttaccgggc
     2881 tcatagatat tgccggcctt tgtcagctct ttccaagtca tatccgggcg tatgccttcc
     2941 ctggaacgac cttgtccaaa gtttctattg aatgctgtca ttcgcccttc acctcctgat
     3001 aagctctctc aatggctttc acattgcctt ctataacttc aggcttagtc ttaaatttct
     3061 tttccagttc gcccttgacg tgttttgcaa attcatcaaa gggcatcagg cctgtagcct
     3121 taataaaagc acccataagc ggtgtatttg ggatgactct gccaatagta tctagagaga
     3181 tctgattggc atccaccgtg a
//
LOCUS       DAONUO010000246         3182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20880_length_3182_cov_3.673489, whole genome shotgun sequence.
ACCESSION   DAONUO010000246 DAONUO010000000
VERSION     DAONUO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20880_length_3182_cov_3.673489"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK768_08555"
     CDS             complement(<1..346)
                     /locus_tag="PK768_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQA61061.1"
                     /translation="MSKVLEIRWHGRGGQGAKTAALMLAETVAQTGKYVQGFPEYGPE
                     RMGAPILAFNRISDDPIHIHSNVINPHIVIVLDATLLGEKVTAGVPEDGIYIVNTTKS
                     PQEVREKLNLKGG"
     gene            complement(724..996)
                     /locus_tag="PK768_08560"
     CDS             complement(724..996)
                     /locus_tag="PK768_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HQA61062.1"
                     /translation="MQMDYAAILAYAFGLLLLYVVGRALIVPLKIVIRLVYNALIGGV
                     VLLLLNFIGGYFGLHIALNPVTALIVGFLGVPGIIMLLVLQYILMG"
     gene            complement(1026..1307)
                     /locus_tag="PK768_08565"
     CDS             complement(1026..1307)
                     /locus_tag="PK768_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HQA61063.1"
                     /translation="MKKHDILRLLSEISSRLLFDNNGDENIDTPEMRLAEQIREAQQE
                     WVRAQKYFQWVSDPELVDHAIFAEEAARRKYIYLLNQAKNQGISTDELK"
     gene            complement(1387..2931)
                     /locus_tag="PK768_08570"
     CDS             complement(1387..2931)
                     /locus_tag="PK768_08570"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HQA61064.1"
                     /translation="MEKFSLPAATGVAAIGLKTGLIFPNDDIAAITAETAKPFVEDND
                     IICVTEAVVARSQNRYISCEELAEDIQRKLNLSPKSTLAVISPIASRNRFALALKALA
                     LATRGGKVILQFSIPFDEVGNQVMDEEFATTRLRLKKVLKSLQEARENTPQLNVLIRE
                     IIAALKLQEIGYSILSIRKITGKGIADLTVRTPEGKLAVAEVTFENLEKAARKAIGIK
                     KDVEGAQQALAITVNLGHHKLTMVDAETLEEPKTYDFGQQLDSYYDPDVVFTNELENI
                     KFSHPITGMDYRDLYMKMIEAGNAEGEVIFTNNPLKVYDRGYINGICIGAVHERGKLK
                     ELFESFGALVPVATLQDIGPEPWGIIGSNVSDFEKGVLKLLPGDADDTAENIKAKIKE
                     LSDKDVEVLIFGDGAYKDPDTGIYELADPYPAIGVSSGLRRASLRTGTKLKLQLDTLH
                     NQGYTREQIEQILKDKQDKVTTESLGTTPRSVTSIVATLADLVAGSADAGTPIVLVRG
                     FNYSKA"
BASE COUNT          822 a          738 c          663 g          959 t
ORIGIN      
        1 ttccgccttt taggttaagt ttctccctta cttcctgcgg gcttttagtt gtattcacga
       61 tgtaaatccc atcttcaggc acccctgctg taaccttttc tcccaagagt gttgcatcaa
      121 gtacaatgac gatgtgggga ttgattacat tggaatggat gtgaatgggg tcatcgctta
      181 ttctattgaa agcgagaatc ggtgctccca ttcgctccgg accatattca ggaaagccct
      241 gtacatattt tcccgtttgt gctacggttt ccgccagcat caaggctgca gtcttggctc
      301 cttgtccacc acggccgtgc cagcggattt ctaacacttt tgacatgaaa agtcctcctt
      361 tcgcaaaaat cctatattat agtttgccta aaaagagcaa tattacccat gttaatgagc
      421 tttgaaaact atccccttta acgaaatatc acaggtacat ggaattcctt ttaggcaaat
      481 atatggcaaa aaataactgt tatagtaaag gtaaacaagc tgccgtctgt tataatacat
      541 gctcctgcgc tatttgatac agtttttatg tattgcattt tctatggatt ttgaaggctg
      601 taacagattt ttcataataa gtataacaga ctgcctgaaa gcggtcaata gatttatgtg
      661 tttttataac aaataaaaaa atcatcactt atgcggtcta acaaagtgat gattttttga
      721 taattaaccc atgagtatgt actgtaaaac tagcagcata attatgccgg gaacaccaag
      781 aaagccaaca ataagtgcgg ttaccgggtt taatgcaatg tgaaggccaa agtagccgcc
      841 tataaaattc aaaagaagca atacgactcc acctatgagg gcattgtaga ccagcctaat
      901 tactatttta aggggcacta ttaaagccct gccgactacg taaagtagca gcagaccaaa
      961 agcataagcc aggatggcag cataatccat ctgcatccta tcacccccat gttttttgta
     1021 ctatattatt ttagctcatc ggtacttata ccttgatttt ttgcctgatt taaaagatat
     1081 atgtacttcc tccgtgcagc ttcttcagcg aaaatagcat ggtccaccaa ttcggggtcc
     1141 gagacccact gaaagtactt ttgggcccga acccattcct gctgagcttc ccgtatttgt
     1201 tccgccagtc tcatttcagg tgtgtcgatg ttttcatcgc cattgttatc gaacaaaagt
     1261 cgtgatgata tttctgataa caagcgcaaa atatcatgct ttttcatgca aactcatctc
     1321 cttattggag attatatcca tttgtatgtc cgcttattca ggagatgagc ttgcacagct
     1381 tgttacttat gccttggaat agttaaatcc tctaaccagt acaataggag ttcccgcatc
     1441 ggcagagcca gccacgagat ctgctaaggt ggcgacgatg ctggtcacac tgcggggagt
     1501 tgtgcctaag ctttcagtcg tcaccttgtc ctgtttatct ttcaatatct gttctatctg
     1561 ttctcgggta tagccttgat tatgcagggt atctagctgt agttttagtt ttgtgcccgt
     1621 cctaagggaa gctcttctga ggccgctgct gacccctatt gccgggtacg ggtctgccaa
     1681 ttcatatatg ccggtatccg ggtctttgta ggcgccatcg ccgaaaatca gcacctctac
     1741 atccttgtcg cttagttcct tgattttagc tttgatgttt tcggccgtat catccgcatc
     1801 gccgggcaaa agtttgagca cacccttttc aaaatcagaa acattggagc cgattatacc
     1861 ccatggttcc ggcccaatat cttgaagggt ggcaacggga accaaggcgc cgaaggattc
     1921 aaagagttcc tttaatttcc cccgctcatg aactgccccg atgcaaatac cgttgatata
     1981 tcccctgtcg taaactttga ggggattatt ggtaaatatg acttcccctt cggcattgcc
     2041 cgcttcaatc atcttcatgt acaggtcccg ataatccatg ccagtgatgg gatgtgagaa
     2101 cttgatattt tccagctcat tggtgaagac cacatccggg tcatagtagc tgtcaagctg
     2161 ctgaccaaaa tcgtaagtct tgggctcttc cagtgtctca gcatctacca tggtcagctt
     2221 gtggtggcct aagtttacgg tgatggccag ggcttgctgg gcgccttcca catctttttt
     2281 gatgccgatg gccttccggg cagccttctc caaattctca aaggttactt ccgccaccgc
     2341 cagcttgccc tccggagtcc tcaccgtcag gtcagcgatg cccttaccgg tgatcttccg
     2401 gatgctcaag atactgtaac caatttcttg gagcttcaga gccgctataa tttcccgtat
     2461 cagcacatta agctgcgggg tgttttccct agcttcctga aggctcttta ataccttctt
     2521 tagccgaagc cttgttgtgg caaattcttc atccatgacc tgatttccca cctcgtcaaa
     2581 ggggatggaa aattggagga tgaccttgcc gcctcttgtg gcaagggcca gggcttttaa
     2641 agccagagcg aagcgattcc tgctggcaat agggcttatg actgccaggg tgctcttggg
     2701 ggataggttt agcttccttt gaatgtcctc agctagctcc tcacatgata tgtagcggtt
     2761 ttgggagcga gccactaccg cttcggtgac gcatatgatg tcattatctt caacaaaggg
     2821 tttggccgtt tctgcggtaa ttgctgcaat atcgtcattg ggaaagatta ggcccgtctt
     2881 taggccgata gctgctacgc ctgtcgctgc aggaagagaa aatttctcca tgggacaaca
     2941 tcctttctgt atcaaagaga atagacatta aattttgcca catcattcat tatatcagta
     3001 aaatccaaaa aatgacaata ataattttaa ctaagcaact tgttgctatt acaatattat
     3061 tataacctat atgtgaaaaa tttacagtta ataagattta aaaatctttt gcctttaaca
     3121 cttaacatac tatgcacaaa agatttggct caaaaagatt taacgggagc aactttgtac
     3181 tc
//
LOCUS       DAONUO010000247         3182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20881_length_3182_cov_3.313080, whole genome shotgun sequence.
ACCESSION   DAONUO010000247 DAONUO010000000
VERSION     DAONUO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20881_length_3182_cov_3.313080"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PK768_08575"
     CDS             complement(<1..1215)
                     /locus_tag="PK768_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HQA61065.1"
                     /translation="MKGELKTEPARSSLFNKFLDIIEIAGNKLPDPVIFFTLLCVIIL
                     ILSWVAESAGLEAVNPGTNETIKAISLLNKDGLIRMLTKMVSNFSSFPPLGLVLVTMV
                     GVGIADKGGLINALMRSAVAGTPASLLVPIIAFIAVISNGAGDCGPIVLPPLAAVIFL
                     SLKRHPLAGMVLAYGACLGGFSANVLVTMTDALAAGFTQQAAQLIDPNYVTNPANNWF
                     FLIAATAIFIPVSTYVNSKIVEPRLGTYTGESVSNEPITETERKGLRAAGIATLVYVA
                     ILALLTLPPNAVLRHPETGGLAVSPFIDSIIPITMLLFLIPGLAYGYASGSIKSSRDI
                     SAMISKSMAELGSFLALAFVASQFLAYFTWSNLGPIIAIKGAEFLQNVGLTGVGMLVG
                     FILISTIINIFIP"
     gene            1483..2481
                     /locus_tag="PK768_08580"
     CDS             1483..2481
                     /locus_tag="PK768_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HQA61066.1"
                     /translation="MKRKIAFIATGGTIASVPTPEGLRPAFTEKEMLDLVPELSSIAE
                     IEGHLIMNIDSSNMQPEDWPVIAKKTLEALASCDGAVIAHGTDTLAYTSSALTYMLTH
                     LKKPVIITGSQRSIGEEESDAARNLIDSFKVAVSGQAGVFVVFGGKIIMGDRASKVKT
                     ESIDAFASINAAEVGFVESDIRWDKELLRRERARLEGIWQKLLKGEGQIDDSDQAKDA
                     VIYDAIDPQVLLIKLYPGMEPDVLLYAKDRGYHSLLIEAFGMGGVCFREPRNLIPAIE
                     ELIAAGITVAVTTQVPFEGADLTRYEVGQRALKAGAISTGDMTREAVLVRLMMKNI"
     gene            2742..>3182
                     /locus_tag="PK768_08585"
     CDS             2742..>3182
                     /locus_tag="PK768_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HQA61067.1"
                     /translation="MLSQKHLLGLENVKKSDIELLLDTAESFKEIMDRDVKKVPTLRG
                     KSIVNLFYEPSTRTRTSFELAGKYLSADTINFTSSASSVRKGESLKDTAKTLEMMGVD
                     AVIIRHSSSGAASYLTRHVSASVINAGDGTHEHPTQALLDMLTVR"
BASE COUNT          889 a          733 c          783 g          777 t
ORIGIN      
        1 ggggatgaag atatttataa tggtactgat taaaataaag ccgactaaca tgccaacgcc
       61 cgtgagaccg acgttttgca aaaactcggc gcccttaatg gcgataatcg ggcccagatt
      121 gctccaagta aaataggcca agaactgtga tgccacaaag gccaaagcca agaatgatcc
      181 cagttcggcc atcgacttgc tgatcatggc agagatatcc ctgctgcttt taatacttcc
      241 ggaggcatac ccgtatgcca aacccggtat taagaacagc aacatggtta ttggaattat
      301 actgtcgatg aagggagata ctgcaaggcc tccggtttcc ggatgacgga ggacggcatt
      361 cggtggtaag gtgaggaggg ccagtatggc aacgtagacc agtgtggcaa tacctgccgc
      421 cctgagaccc ttccgctctg tttcggtaat gggttcattg gatacgcttt ctcctgtata
      481 agtgcccaag cgaggttcaa caattttgct gtttacatat gttgatacgg gaataaatat
      541 ggcggttgct gctatcagga aaaaccagtt gttggcgggg tttgtcacat aatttggatc
      601 tatcagctgt gcagcctgct gcgtaaatcc tgcggcaagg gcgtcggtca tcgtgaccag
      661 gacatttgcc gaaaagccgc ccaagcaggc gccgtaagcc aaaaccatgc cggccagggg
      721 atggcgcttt aagctcaaaa aaataaccgc agcaaggggc ggcaatacta taggcccgca
      781 gtcaccggcg ccgttactga ttacggcaat aaaggcgatg attggcacca gcagggaagc
      841 cggcgttccc gcaacagcag aacgcataag ggcatttatt agtccacctt tgtcggcaat
      901 acctacgccc accattgtca ccaggacaag gcccaggggt ggaaagctgc tgaaattact
      961 aaccattttg gtcagcatgc gtattaaacc atccttattc aagagactga tggctttaat
     1021 ggtttcattg gtaccggggt ttacggcttc caaacctgca gactctgcaa cccaagacag
     1081 aattaatatg attacacaca gcaaggtaaa aaatataact gggtccggca gcttattacc
     1141 ggctatttcg ataatgtcta aaaacttgtt aaacagacta ctgcgggctg gttcagtttt
     1201 tagctcgcct ttcatgataa ttcatctcct ttaatgtaaa attttgattc agatgattag
     1261 ttatacgaac tttgtccctc ccttcactta cataaaagat tatattactc cgtttattta
     1321 attgcaaatg aaatcacaat atttttcata tgtttatatg tcccaactaa cgatatagct
     1381 gcactactta cataattcac aatattacat gtttattgca ggaggaataa ttgattctgt
     1441 ggagaactaa gatataaccc atgcaagatg gaggtaacaa ccgtgaaaag gaaaattgca
     1501 tttatagcta cgggcggcac tatcgctagt gtcccgactc ctgaaggctt gcggcctgcc
     1561 tttacggaaa aagagatgct agacttggta cccgagcttt ctagtattgc cgaaattgag
     1621 ggccatctca ttatgaacat cgacagttcc aatatgcagc ctgaggactg gccagtaata
     1681 gccaaaaaga ccctggaagc cctggcaagc tgtgacggtg cggtcatcgc ccatggcacc
     1741 gataccctgg catacacttc ttcggctttg acctatatgc tcacacactt aaaaaagccg
     1801 gttattatta ccggttccca aaggtccata ggagaagagg aaagcgatgc tgctcgcaat
     1861 ctcattgaca gctttaaggt ggccgtctcg ggacaggccg gggtattcgt ggtattcggc
     1921 ggcaaaatca ttatggggga cagggcttcc aaggtaaaga ccgagagcat tgatgccttt
     1981 gccagcatca atgcagcgga agtgggtttt gttgaaagcg atatccgctg ggacaaagag
     2041 cttttgcgaa gagagagagc taggctcgaa ggtatctggc agaaactgct gaaaggcgaa
     2101 ggccaaatcg atgatagcga ccaagccaaa gatgccgtaa tttacgatgc cattgacccg
     2161 caggtgctgc ttatcaaact ttaccctggc atggagcccg atgtactgct atatgcaaag
     2221 gacaggggct atcattcgct gctcatcgag gcttttggca tggggggagt ctgctttcga
     2281 gagcccagga atttgattcc cgcaattgag gaattgatag cggcagggat caccgttgcc
     2341 gttactacac aggtgccctt tgaaggggct gatttgacca ggtatgaagt tggccaaagg
     2401 gcgctcaagg ccggtgccat ctccaccggc gacatgaccc gggaagctgt cttagttaga
     2461 ctgatgatga aaaatatttg acaaggcttc tttgctatgg tatatttaat aaaataaaaa
     2521 ctgaaagcaa cctttaaatc tggtccagtg aggccgggaa ggttaagcgc atgagaactt
     2581 gcagcaactt ctagggacac gaatgccttt ttccgcctcg gaaaaaggct tttttgataa
     2641 actcagctta gctgccatac ttatgcggaa aattccccaa acctaccctg cccttactgg
     2701 gcagggtttt ttagtaaact taacttggaa ggaggagctt tatgctcagc caaaaacacc
     2761 tcttgggcct tgaaaatgtg aaaaaatccg atatcgaact tctccttgat actgcggaat
     2821 cctttaaaga aatcatggac agggacgtaa aaaaggttcc caccctccgg ggaaaatcca
     2881 tagtgaacct cttttacgaa cccagtacca ggacccggac ttcctttgag cttgcaggca
     2941 aatacctcag cgccgacacc atcaacttta catcttctgc aagcagtgtg cgaaagggtg
     3001 agagcttaaa ggataccgcc aaaaccctag agatgatggg ggtagatgcc gttataatca
     3061 ggcacagcag ctcgggggcg gcaagttatt tgacccgcca tgtttcagca tcggtgataa
     3121 acgcaggtga cggcacccat gaacatccca cccaggcttt gcttgacatg cttacggtga
     3181 gg
//
LOCUS       DAONUO010000248         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_20940_length_3175_cov_3.287179, whole genome shotgun sequence.
ACCESSION   DAONUO010000248 DAONUO010000000
VERSION     DAONUO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20940_length_3175_cov_3.287179"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1311)
                     /locus_tag="PK768_08590"
     CDS             complement(184..1311)
                     /locus_tag="PK768_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HQA61068.1"
                     /translation="MLKRIISLLIVMSLLLGVVGCGGGSQKSEAEAPAGDDWKIGILT
                     GTSSQNEEEFVAGRELVEKYGEDRVIHKTYPDNFTKEQETTITLAMNMAADPSVKAII
                     FCQAVPGTAAAFEMIREQRDDILLIAGLPQEDPAIMSELSDIVIDTDNLRRGETIMEL
                     AHQMGAKKFIHYSFPRHMSIELLAQRRDNFQKKADELGIEFIEVSAPDPMGEGGIPAT
                     QQFILEDVPRQVAEHGKDTAFFSTNCAMQEPLIKKVLDEGAIFPEQCCPSPYHGYPGA
                     LGIEIPEDKVGNVDFIISEITAKVAEKGNSGRMGTWTVPAAIALIRACGAYAVEYAQG
                     NVERQDIDAFVDFLRQEAGDILVQTYKADNFLLFVGESQVF"
     gene            complement(1414..2511)
                     /locus_tag="PK768_08595"
     CDS             complement(1414..2511)
                     /locus_tag="PK768_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQA61069.1"
                     /translation="MKLERIKKLAESLKRENIGAVIVGPSPDLEYLTGLTPSNDERFK
                     GLFVLSDASFFYIAPSLYLEETQKGIGDSAKIYVWYDEEGFLGVLRQARDDFELNGLR
                     IAISDGISAVHLLDIQENIDAEFVRGDILLGELRMIKDEEEMENLRKASEIIDDVVKD
                     LADFIRPGIRERDIRRKIEELCLEKGASGLSFDPIVASGPNSSMPHYNGDSRIIQERD
                     IVLLDLGCKYNGYCSDTTRTFFIGEPSAKEKEVYAAVLEANLAAEAFVKEGVACEEVD
                     KVAREVLRKAGLDKFFITRTGHGIGVNVHEFPNIVANNKQLLKNGMAFSIEPSVCIPG
                     EFGIRIEDIVLVNDGKAEILNKFPKEMLVIK"
     gene            2733..>3175
                     /locus_tag="PK768_08600"
     CDS             2733..>3175
                     /locus_tag="PK768_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HQA61070.1"
                     /translation="MNTHKRREQIVELLSSSHEPISGKELAKVFGVTRQVIVQDIAIL
                     RASGKDIIATAQGYLMKGPLPICTKQIAVCHKAEDTREELLTFIECGCKVIDVIVEHP
                     LYGELHGLLMLSNSRDVDNFIRAVKEHKAGLLSQLTDGVHLHTVEA"
BASE COUNT          831 a          758 c          601 g          985 t
ORIGIN      
        1 actagggcat ggacttcccc tgggtggaca gttaagctca cattttttaa gactttattt
       61 ccgtaatatt ccttgctaat atctagcaac tgtaaaatcg gttcactcaa attttaccct
      121 cctcgtgctt caatatttaa ggttgcctat caggcaacct taaatattga tgtatatctg
      181 ctattagaat acctgtgatt cgcctacaaa gagaaggaag ttgtcagctt tgtatgtctg
      241 tactaaaata tctcctgctt cctgtcttaa gaaatcaaca aatgcatcaa tatcctggcg
      301 ctctacattg ccctgggcat attctacagc ataagcaccg catgccctaa tcagtgcaat
      361 tgcggccgga accgtccagg tacccattct gcctgaatta cccttttctg ctaccttcgc
      421 tgtaatctcg cttataatga aatcaacatt accgacttta tcctcaggta tttcgatacc
      481 taaagctccg ggatatccgt gatatgggct tgggcagcac tgttcgggga aaatagcccc
      541 ttcatcgaga actttcttga ttaatggttc ctgcatagca cagttcgtgc tgaagaaggc
      601 cgtgtctttg ccatgctctg cgacctgtct tggcacgtct tctaagataa attgctgtgt
      661 tgcaggaatt ccaccttcac ccatggggtc gggtgctgat acttcgataa actctatgcc
      721 aagttcatca gcttttttct ggaaattgtc tcttctctgg gccaaaagtt caatggacat
      781 atgtctgggg aatgaatagt gtatgaactt tttagctccc atctgatggg caagttccat
      841 tatggtttct ccacgtctca ggttgtctgt atcgattact atatcagata attcgctcat
      901 tatggcagga tcttcctgtg gcaatcctgc aatcaagagg atgtcatcgc gctgttctct
      961 aatcatctca aatgctgctg cagttcctgg cactgcctga caaaagatta ttgcttttac
     1021 acttggatct gcagccatgt tcatggccag tgtaattgta gtttcctgtt cttttgtaaa
     1081 gttatcaggg tatgtcttgt gaattaccct gtcttcgcca tatttttcta ctaactctct
     1141 accggcaaca aactcctctt cgttttgaga actggtgcct gtaagtatac caattttcca
     1201 gtcatcgccg gccggtgcct cagcttctga cttctggctg ccgccaccac atccgacaac
     1261 accgagaagt aaagacatta caatcaacag cgaaattatt ctttttaaca tttttttcat
     1321 tctccccctt taaattaata aacaatcaat ttatcagtta tatatctgct aaaactttta
     1381 tctatctctt taaacgtccc cccttttttt tgttcatttt attaccaaca tctccttggg
     1441 aaatttgttt agtatctctg cttttccatc atttaccaag actatgtctt ctatgcggat
     1501 tccaaattct cccggaatgc atacactagg ctcaatacta aatgccatgc catttttcaa
     1561 gagctgctta ttatttgcaa ctatattggg aaattcgtgg acatttacgc caataccatg
     1621 gccggtcctg gtaatgaaaa acttatcaag gcccgctttc cttaacactt cccgggcgac
     1681 cttatccact tcttcgcagg caacaccttc tttaacaaag gcttccgctg ccagatttgc
     1741 ctcgagaact gcggcataaa cttctttctc ctttgcgctg ggctctccta tgaaaaaggt
     1801 acgggtagta tctgaacaat agccattata cttacatccc aagtcaagaa ggacgatatc
     1861 gcgctcttgg ataatcctgc tgtcaccgtt atagtggggc atggagctgt ttggccctga
     1921 agccactatg ggatcgaaag aaaggccgga agcacccttt tcaaggcaaa gttcttcaat
     1981 ctttctcctt atatcccttt cccgaatgcc gggccttata aaatccgcca agtcctttac
     2041 aacatcatca atgatctcgg atgcctttcg tagattctcc atttcttctt catctttgat
     2101 cattcggagt tctcccagta aaatgtcccc cctgacaaat tcggcgtcaa tattttcctg
     2161 tatgtcaagg agatgcactg ccgaaattcc atcacttatc gctatgcgca ggccatttaa
     2221 ctcgaagtca tcccttgcct gccttagtac tcctaaaaaa ccttcctcat cgtaccacac
     2281 gtatattttt gcactatcac ctataccctt ttgagtttcc tcgagataaa gcgatggcgc
     2341 tatgtagaag aagcttgcgt cggacaaaac aaataacccc ttgaatctct catcgtttga
     2401 aggggtaagg cctgtcaaat attctaaatc gggtgagggg ccaacgataa cggcaccgat
     2461 gttttccctt ttaagagatt ctgccagctt ttttattcgc tcgagtttca aaaaaaagcc
     2521 tccttatcat ttcactcaat tatatagcct aataaaataa atagatgtcc tatttgttaa
     2581 ttttacagga aaatgacttg tttttcaagc aaaaatgagg ttacataatt tgattccaat
     2641 ggaaatttaa ggattctcgc ttgctaatct taatctttta gtgttgcaaa aggtttggta
     2701 taataggtac aaagctcgtg gaggtaagtg aaatgaatac tcataagcga agggaacaaa
     2761 tcgttgaatt gcttagcagc agccatgaac ccatatcggg caaggagctt gccaaggtct
     2821 tcggtgtcac acggcaggta atagtacagg atatagccat tctcagggct tcgggaaagg
     2881 atatcatagc tactgcccag gggtacttga tgaaaggtcc cctgcccatt tgcaccaagc
     2941 aaatcgccgt atgtcataag gcagaagata cccgtgaaga gctcttgact tttattgaat
     3001 gcggctgcaa ggtaatagat gttatagttg aacatcctct atacggcgaa ctccacggat
     3061 tattaatgct ttccaactct cgggatgtgg acaatttcat ccgggcagtc aaagagcata
     3121 aagcaggttt gctctcacaa ctaaccgacg gcgtccacct gcacactgtt gaagc
//
LOCUS       DAONUO010000249         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21137_length_3150_cov_3.565105, whole genome shotgun sequence.
ACCESSION   DAONUO010000249 DAONUO010000000
VERSION     DAONUO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21137_length_3150_cov_3.565105"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PK768_08605"
     CDS             complement(<1..1297)
                     /locus_tag="PK768_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQA61071.1"
                     /translation="MIHLRKKLALIMIVALVFSQGLLPTSAAYGETKGVVLGSSEAPQ
                     NGSAREIIETTYPQNNQTDVEVKPTIKIIFKFPVEIKDTDKIVLDSGGERYPLGPSEI
                     YLLDDERTLCIDVGRIGRPPLRRGTLYHLTLSSGAVKLKEHEIANEDISISFVTRTEG
                     QSPKVTAYSSLATGADDIRSLSGTRLSREAFIHIIFDRNIKLEKNTDKDKFLEGIKLY
                     RIPKPQETDESGNDKAFEFEPGITEADLRGEANRQEIVVEDVEIINNILRIKPKWPLL
                     NLNQYHMTIEKELIEDLNGYALEEDVDFYFWTAPAPEKPEFFWEHIEGTLPGSIQDDP
                     STGGKSCTVVGTPAYGPDTPLVFIIGGEAIPKAGDVSSLKRITLVEGYEPSSTVKISR
                     VRFEYYNEGDVKNTKLFIYPENILDAGKYYVLTLPEVIQD"
     gene            complement(1488..>3150)
                     /locus_tag="PK768_08610"
     CDS             complement(1488..>3150)
                     /locus_tag="PK768_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQA61072.1"
                     /translation="ILFIVISIVFILSSLMIQSSYKLLTQTLGSTALEVAAVTSATIN
                     PSEFTKIKTVDDTKTDIYKAMQEKLSYIREKSGSKYLFTIRKNDAGEYIYVVDGSVKP
                     AEFGDVEKAYEEFDRAYAGDSFINNKLQISQEHGVLATAFHPIIHNGEIIGIVGVGYD
                     VEQGYLVLKEMINNIVVLAIVLAAVALIIGFFFSKKISRPLELLAAVTNKVSNFDLSV
                     ETIDINSRDEIGNLSKAFNLMINSIKEITKDTKYSTNTVLDMSHSLYTIADNVNSQVN
                     EISEAIHHVAESITEQAHDIDRGSKQTDELAESIEKVASSINDITDIFSQVYNLNDRG
                     SQAIDSLIQKSKEEEEAVLEAETNIKDMDESSQKISVILDTVEQISDQTNLLALNASI
                     EAARSGEHGRGFAVVAEEIRKLADESAKSTEEIKKLITAIQDRSSRAVKSMEISKKIA
                     EEQNSIVEETGKVFRELSNAIKKLAENIETIEVLNSDMNHKKDAIVTMIRAVQESVEE
                     ISAVSQETSASSQEILFSVNQLKEHSRTLNDLAEELGVKVDQFKT"
BASE COUNT          773 a          691 c          634 g         1052 t
ORIGIN      
        1 ggtcttgaat cacttccgga agggtcagca catagtactt ccctgcatcc agtatatttt
       61 cgggatatat gaacagtttc gtatttttga catcgccctc attataatat tcgaagcgaa
      121 ccctagaaat ctttaccgtg ctgcttggct cataaccttc aactaaggtt attctcttaa
      181 gggaggatac atctccggcc ttgggaattg cctctccccc tattataaaa acaaggggtg
      241 tgtcaggacc gtaggcagga gttccgacga cagtgcaaga tttaccacct gtagaaggat
      301 cgtcttgtat gctgccgggc agcgtccctt caatatgttc ccaaaaaaac tctggctttt
      361 cgggagcagg tgcagtccaa aagtaaaaat ccacatcctc ttccagggca tatccgttta
      421 gatcttctat cagttccttt tcaattgtca tgtgatactg gttgagattt agcaggggcc
      481 acttgggctt tatgcgcagg atgttgttga ttatctccac gtcttctact actatttcct
      541 gccggttggc ttctcccctc aaatcagctt ccgtgatgcc cggctcgaat tcaaaggcct
      601 tgtcattacc tgattcgtcc gtttcttggg gcttgggtat gcgataaagt tttatccctt
      661 caaggaactt atctttgtca gtatttttct caagcttaat attgcggtca aaaattatat
      721 gtatgaaagc ttcacgggaa agcctggtcc ccgaaaggga cctaatgtca tcagcacccg
      781 ttgctaaaga agagtaagct gtgactttgg ggctttggcc ctcggtccgg gtgacaaagc
      841 ttatggagat atcttcattt gcgatttcat gttctttaag tttaaccgca ccacttgaca
      901 aagtgagatg gtacagtgtg ccgcgcctta aggggggcct tccgattctc cccacatcta
      961 tgcaaagggt cctttcgtcg tccaggaggt atatttcact tgggcccaac ggataccttt
     1021 ctccaccgga atccaagacg attttatccg tgtcctttat ttcaacgggg aatttaaata
     1081 taatttttat tgtcggtttt acttccacat ccgtttggtt gttttggggg taagttgttt
     1141 cgataatttc ccgagccgaa ccattttgag gagcttctga cgagcctaat acgactccct
     1201 tcgtttcacc gtaagcagca gatgtgggta gtagaccctg tgaaaaaacg agggcaacaa
     1261 tcattattaa ggctagcttt tttcttaaat gaatcacggc ctttacctcc atgcataagc
     1321 tttccctggg tttttcttat ttttccctat tataccataa atttggacaa attttttagc
     1381 tgttttatct gtaacaaaaa agtaatgttt gcttaaaagc tacataaaaa acactgccgc
     1441 ttgtatacgc ggcagtgtaa atttgccagt aaaacaatgg tggcatatta ggttttaaat
     1501 tggtctactt ttacacctag ttcttcagcc agatcgttta gggttctgga gtgctctttg
     1561 agttgattta ctgaaaacag tatctcctgg gaagaagctg aagtctcctg ggataccgcc
     1621 gaaatttctt ccacagattc ctgaactgcc cttatcattg ttacgatggc atctttttta
     1681 tggttcatgt cagaatttag cacttctata gtttctatgt tctcagctaa ttttttaatg
     1741 gcatttgaaa gctctctgaa aaccttgcca gtttcttcta caatgctgtt ttgttcctct
     1801 gcgatttttt tgctgatttc catggatttt acagctctac tagacctgtc ctggattgca
     1861 gtaatcagct ttttaatctc ttccgttgat tttgccgact cgtcggccaa ttttcgtatc
     1921 tcttcagcaa ccacggcaaa accacggccg tgttcaccag agcgtgctgc ttcgatgctg
     1981 gcatttaacg ccagaaggtt tgtttgatcg gatatttgtt ccaccgtgtc cagaatgaca
     2041 cttattttct gggaactctc atccatgtcc ttaatattcg tttccgcttc aagtacggcc
     2101 tcttcctctt cctttgactt ctgaatcaac gagtcgatgg cttgggaacc cctgtcgttt
     2161 aaattgtaca cttggctgaa aatatcagta atgtcgttaa ttgaactggc caccttttct
     2221 atgctttcag ccagctcatc cgtctgtttt gagcccctat caatgtcatg ggcctgctcc
     2281 gttatggatt cagcgacgtg atgaattgct tcagatattt cattgacttg cgagtttaca
     2341 ttgtcggcaa ttgtgtacaa ggaatgggac atatccagga ctgtgttagt tgagtacttg
     2401 gtgtcttttg taatttcctt tatgctattt atcattaagt taaaggcttt gcttagattg
     2461 cctatctcat cccttgagtt tatatcaatt gtttcgacac ttaaatcaaa attcgatact
     2521 ttgttggtta cagcagccaa aagttccaga ggacgggaaa tctttttgga aaagaagaat
     2581 ccaattatta gggcaacagc tgctaacact atagccaaaa ctacaatatt gttaatcatc
     2641 tcttttaaaa ccaggtagcc ctgctctaca tcatagccaa cgcctacaat gccgattatt
     2701 tcaccgttat gaattatggg atgaaaagca gttgctaata cgccgtgctc ctgactaatt
     2761 tggagtttat tgttgatgaa ggaatcccct gcataggccc tgtcaaattc ttcataggcc
     2821 ttttccacat ctccaaactc agccggtttt acagatccgt ccaccacata aatatattca
     2881 cccgcgtcat ttttcctgat ggtgaatagg tattttgagc cggacttctc ccttatgtaa
     2941 cttaattttt cctgcatggc tttgtaaata tcggtttttg tgtcatctac agtctttatt
     3001 ttggtaaatt cagaagggtt tattgtggca gaagttactg cagctacttc cagtgctgtg
     3061 ctccccaatg tttgggtcag caatttatag ctggattgaa tcataagtga tgataggata
     3121 aaaacaattg agatgacaat gaatagaatt
//
LOCUS       DAONUO010000250         3136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21256_length_3136_cov_3.590717, whole genome shotgun sequence.
ACCESSION   DAONUO010000250 DAONUO010000000
VERSION     DAONUO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21256_length_3136_cov_3.590717"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK768_08615"
     CDS             complement(<1..284)
                     /locus_tag="PK768_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQA61073.1"
                     /translation="MERTNWYLLENEEVSSKLSTDLEKGLSSELAKERLKSHGYNELI
                     SKEGPTIWQMLLSQFQDFLVLILIGASLVSALIGEVTDSVVIILIVVLNAT"
     gene            707..1255
                     /locus_tag="PK768_08620"
     CDS             707..1255
                     /locus_tag="PK768_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HQA61074.1"
                     /translation="MIFAVLFAVTLLLLGYIFGQLKITERNKINYIARAAVIATIYTL
                     ITYLFKPISYGVIQIRVSEALTLLPLVESAAIPGLFIGCLLANILGGMGPWDIYFGSL
                     ITLIAACITAKMPGPVSGSLPPIVLNAFGISYYLSIIYNVPYWITVLYIGIGQLISVA
                     ALGIPLYHFIQRTSLKDFFKKE"
     gene            1275..2357
                     /gene="mltG"
                     /locus_tag="PK768_08625"
     CDS             1275..2357
                     /gene="mltG"
                     /locus_tag="PK768_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQA61075.1"
                     /translation="MNIKYLYHRFIESSAVKYIKDRKKIVLTIVIIAIIPVLALKWYQ
                     SMLSPKNAADTILVEFEVESGITAKQLAAKMQEHGMIKNQFAFILYAKLEGQDNRIKA
                     GKYFLSPSMSPEEILDKLVKGEMINEDIKVTIPEGSTVNTIAAIFSEKGLVAEEDFIK
                     SLDIDKYKNKYTFLKDFPPNATLEGFLFPDTYLMPPGKNPEFYIDILLNRFQDVYFNK
                     VDSVIKENKIELSTYQVVTLASIVEAEAKLERERPIIAGVFHNRLNIGMPLQSCATVA
                     YALGEHKEELSLEDLEVDSPYNTYKVSHLPPGPIGAPGLSSMLAVANPAKVDYLYFVA
                     KGDGSHIFNVTYQEHLKAQRMIESQK"
     gene            2531..>3136
                     /locus_tag="PK768_08630"
     CDS             2531..>3136
                     /locus_tag="PK768_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQA61076.1"
                     /translation="MQKTGPLVDKLRGGIYDTKGKSLTGSFIASFAVIAPNWLSAQEK
                     QLLVENDILDSVDSSEIETIQLTPENSHIVYDLKGRTPGIAIYNKEMRYGPDALATHV
                     VGFQGQTGIEKTFNSFLESDIKRYYVIRDGLGQPIGGIALQKPMTEAWGIKLTIDRKI
                     QKIVEDIMDESIYKGAVVILDTNSGEVLAMASRPNYKQYKLE"
BASE COUNT         1057 a          544 c          604 g          931 t
ORIGIN      
        1 gtggcattta aaacgacgat gaggattatt actacggaat ctgtaacttc cccaatcagt
       61 gcggatacga gacttgcacc tatgagtatc agcacgagga aatcctggaa ttgggaaagc
      121 agcatctgcc aaattgtagg tccttcttta cttatcaatt cattgtaacc gtgagactta
      181 agtctttctt tagctaattc cgaggacaga cctttttcta aatcggtgct tagttttgaa
      241 gagacttcct cattctccaa caagtaccag tttgttcttt ccaacaaata ccctcctcat
      301 atttagtact agtagtatat gaagattaaa gcattttatt taaagcatat caccacctta
      361 tgcacttatt ataaatataa gttccctaat ctgtctatac aggaaagatt aaggtttgta
      421 tctgtgctta taataaaaaa acctttaata taatcatacg aaattatatt aaaggtcttg
      481 tcttcagtaa aactgaatga taaagccagg ctgaaaagcc gtgatggtga ctttatcaaa
      541 atgactactc ccctttaatt gaaaactctg atatgtattc aattaattgt ctacgttaaa
      601 aaatatatag tataatctaa ctaaagtcaa tagcaaaatg tttgactagt tcaatcagta
      661 aggttaaact agaatatgat ggaaatagaa aaaaaggaag attaccatga ttttcgctgt
      721 tttatttgcg gtaacccttt tactcctggg ttatatattt ggccaattga aaataacaga
      781 gagaaacaaa ataaattata ttgcgagagc agcggtgatt gcaactatat atacactaat
      841 tacctacttg tttaagccca taagttatgg tgtaattcaa ataagggttt ctgaagcttt
      901 aacattgcta cccctagttg aatcggctgc tatccccggg ctttttatag gctgcttact
      961 cgccaatatt ttggggggta tggggccatg ggatatatat tttggtagct taatcacatt
     1021 gatagcagca tgtatcacag ctaaaatgcc cgggcccgta tcaggttcgc ttccacctat
     1081 tgttctaaat gcatttggta tatcttatta tttatccatc atctataatg ttccctattg
     1141 gataacagtt ttatatattg gcataggtca gctcatatcg gtagcggcct tagggattcc
     1201 attgtatcat tttatacaaa ggacaagcct taaggatttc ttcaaaaaag aatagcaaaa
     1261 gaggtgtaat cattttgaac attaaatacc tctaccatcg ctttattgaa agcagtgcgg
     1321 taaaatacat aaaagacagg aaaaaaattg tattaactat tgttattata gcaataatac
     1381 cggttttagc tttaaagtgg tatcaaagta tgctttcccc taaaaatgca gccgatacaa
     1441 tccttgttga atttgaggtt gaaagcggca ttactgcaaa gcaactggca gcgaagatgc
     1501 aggagcatgg catgataaaa aatcaatttg ccttcatttt atatgcgaag cttgaggggc
     1561 aggataatag aataaaggca ggcaaatatt ttctgagccc ttcaatgtca ccggaagaaa
     1621 tcctcgataa actggtgaaa ggagaaatga tcaatgagga tataaaagtg acaattccag
     1681 aaggcagtac agttaatact atagctgcta tttttagtga aaaaggcttg gttgccgaag
     1741 aagatttcat taaatcactt gacatagaca aatacaaaaa taagtatact ttcttgaagg
     1801 attttcctcc aaatgcaact ttagaaggtt tcctatttcc cgatacttac cttatgccac
     1861 cgggcaagaa tcccgagttc tacatagata tactgttgaa tcgctttcag gatgtgtatt
     1921 ttaataaagt tgatagtgtc ataaaagaaa acaaaatcga gctttctact tatcaagttg
     1981 taacattggc ttctatagtt gaagctgagg ccaaactgga aagggaaaga ccaataatag
     2041 caggggtttt tcacaacaga ctgaacatag gaatgccact gcaatcctgt gcaacggtgg
     2101 cctatgcatt aggtgaacat aaggaagaac tgtctctaga agatctcgag gttgattctc
     2161 cttacaatac atataaagtc agccatttac cgcctggccc aataggggcg cccggtcttt
     2221 cttcaatgtt agcagtggcg aatcccgcaa aggttgatta tctgtatttt gtagccaaag
     2281 gagatgggag tcacatattt aacgtaacct atcaagaaca tcttaaagct caaagaatga
     2341 tagaaagtca aaaataatat aggaataaaa atccatttta agggcattat actcatgggt
     2401 gaattaagtg ggaacgaata agcgtatgta tttattgacc ttcataattt ctaccatgag
     2461 tataatttta ttatttagac ttttttacat tcaaatagta aaaggcccgt ttctagcaga
     2521 agccgctttt atgcaaaaaa ccggcccatt ggttgataaa ctaagaggag gcatatatga
     2581 cacaaaggga aaatccctga cgggtagttt tattgcaagt tttgccgtca tcgcacccaa
     2641 ttggttatca gcacaagaaa aacagctact ggtggaaaat gatattttag attcagtaga
     2701 ttcaagcgag atagaaacta ttcagttgac accggaaaac tcccatatcg tatacgatct
     2761 taaaggtaga actcccggta tcgccattta caacaaagaa atgagatacg gtcctgacgc
     2821 tttggctact catgtagtag gctttcaggg ccaaaccggg attgaaaaaa cttttaacag
     2881 ttttcttgaa agtgacataa aaagatatta tgtaattagg gatggattgg gtcaacctat
     2941 tggcggtata gcattacaaa aacctatgac ggaagcctgg ggaataaagc tgactatcga
     3001 taggaaaatt caaaaaatcg ttgaagatat aatggatgaa agcatttata agggggcagt
     3061 tgtcatactt gatacaaatt ccggcgaagt acttgccatg gccagcaggc ccaattataa
     3121 acaatataaa ctggaa
//
LOCUS       DAONUO010000251         3113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21477_length_3113_cov_3.749836, whole genome shotgun sequence.
ACCESSION   DAONUO010000251 DAONUO010000000
VERSION     DAONUO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21477_length_3113_cov_3.749836"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /gene="dnaX"
                     /locus_tag="PK768_08635"
     CDS             complement(<1..1704)
                     /gene="dnaX"
                     /locus_tag="PK768_08635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQA61077.1"
                     /translation="MSYVALYRKYRPQTFDDIVGQSAVVTTLKNQLSSGKIGHAYLFC
                     GMRGTGKTSTARVFAKALNCEKGPTPNPCNVCASCRAINSGSMMDVIEMDAASNRGID
                     DIRDLREKVNFAPSVGRYKIYIIDEVHMLTTEAFNALLKTLEEPPRYVVFILATTEPN
                     KLPQTILSRCMRFDFTRISTTEIVERLRKVVRDLGIEAEDRALVEIARSSQGSVRDAL
                     SLLDKAVAYGEARLEHQDILTLLGAVNQEVYYDISKVVLQGKSGVLLEILDRLAKQGK
                     DLFRFLEDLLEHFRNLLMVSIDADRKLIDVIDEDYRELKKIAQGYTKEKLLSILGILK
                     DAANDAKWSTQPRIIIEAALVKLTLLELWEEQEGYISRIQQLEEQLAAMQRLLENIKA
                     PVLQSAIQDLDLEPERDTVKNHAAPLESIRNMDIINSKEQTEKPAESEKSKKQAAKSD
                     DADIISRLQKEWPKVLEHLGDKGKMKLLSTLEAGKMVPAKVEQGKLFLLNRGESFFEE
                     IILSDKKIIEETIKQVSNVDIIIKGFVGEESISQDEERDLQNEEGRELSDEEYRQKVI
                     EF"
     gene            complement(1783..2047)
                     /gene="ffs"
                     /locus_tag="PK768_08640"
     ncRNA           complement(1783..2047)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK768_08640"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            2410..>3113
                     /locus_tag="PK768_08645"
     CDS             2410..>3113
                     /locus_tag="PK768_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQA61078.1"
                     /translation="MHSIEDIKAEEQRRITANEIEEILSKKYKVKRIVADKNLIDNLL
                     KSKVNFVFNLSTGIKGESRQSQVPALLEMLGIPYVGSGVLAHALALNKAFAKKIFKFH
                     NVPTPYFQEFYTGKEDLDPNLKFPAIVKPACEGSGFGIHKDNLVNTDKDLYKRVERLL
                     KHYQPPAIVEEYIDGREYTVGIIGNGNDKTVLPIVEIDFDKVTAEFDKFNTFESKHGQ
                     GGQKQILCPAKLTEEQ"
BASE COUNT          851 a          710 c          607 g          945 t
ORIGIN      
        1 aaactcgatg actttttgac ggtattcctc atcggacaat tctctgcctt cttcattttg
       61 caggtctctt tcctcatcct gcgaaatgct ttcctcaccg acaaatccct taatgattat
      121 gtccacattc gatacctgtt ttatagtctc ttctataatc tttttatccg ataatattat
      181 ttcttcgaag aaggattcgc ccctgtttag caaaaacaat ttgccctgct ctactttggc
      241 cggaaccatt ttccctgcct cgagtgttga cagaagtttc atcttacctt tatcgcccaa
      301 atgttcaagg accttaggcc attctttttg cagtcgactg attatgtcag catcatctga
      361 ttttgccgcc tgttttttgc ttttttcgga ttcggctggt ttttctgttt gctctttact
      421 attaattata tccatgtttc ggatagattc tagcggtgcg gcatgatttt ttactgtatc
      481 cctttccggt tccaggtcaa gatcttgaat cgctgattgc aaaaccggtg cctttatgtt
      541 ctcaagcagc ctttgcatag ctgcaagctg ctcttccagc tgctgtattc tggaaatgta
      601 accttcctgc tcttcccaca attccaatag tgtaagttta actaaggctg cttcgattat
      661 tatccgaggc tgcgtggacc attttgcatc atttgcagca tctttcaaga tacccaagat
      721 ggacagcagc ttttccttgg tgtatccttg cgcaatcttt ttaagctcgc gataatcttc
      781 atctataaca tcgataagct ttctgtccgc atcgatactg accatcagca aattcctgaa
      841 gtgttccaac aaatcttcta aaaacctaaa caaatccttg ccctgcttag caagccggtc
      901 tagtatctcc agcagtacgc cgcttttacc ttgcaatacg accttagaaa tatcgtaata
      961 tacttcctga tttacggccc caagcaatgt caaaatatcc tggtgctcta atcttgcctc
     1021 accgtatgct actgccttat caaggaggct taaggcatcc ctgaccgacc cttgggagct
     1081 tcgggctatt tcaaccaatg ccctatcctc agcttcaatg cccaagtccc ttactacctt
     1141 tctcaacctt tcgactattt cggttgtgct tattcgcgta aagtcaaatc tcatacatct
     1201 agatagaata gtctgaggta gtttgttggg ttcggtagtt gccagaatga aaaccacata
     1261 tcggggaggt tcttcaaggg tcttcaaaag ggcattaaat gcttccgttg tgagcatatg
     1321 gacttcatct atgatataaa tcttgtacct acccaccgat ggcgcgaaat ttaccttttc
     1381 cctgaggtct cgaatatcat cgatacccct gttcgatgcg gcatccattt caataacatc
     1441 catcatactg ccgctattga ttgctctgca cgatgcgcat acgttacagg ggtttggagt
     1501 aggccccttt tcacaattga gggccttggc aaaaacccta gcggtgctgg tcttgcccgt
     1561 tcccctcata ccgcaaaaca ggtaggcgtg gccgattttg ccgctggaca gttgattttt
     1621 caatgtggtc acaacggcac tttgacctac gatgtcatca aaagtttggg ggcggtactt
     1681 tctataaagg gccacataag acatgaaaat ctctcccgag tatgtaaaat caaacttgat
     1741 attttaaaag ccgccgatac tgtcggcggc taaaacttta tgccgtgcac cctccgtcga
     1801 tctatccctc cgagcgttac aatgacagtt agctccgacc aggctgcccc gcggcacatg
     1861 gaagggcttc cttaccgctg cttcctcccg gacctgacgg ggttcgaaag gttccattgc
     1921 gcaggacccg gccatcgtca ccacttgtca aagccagacc ccacagggaa aagacctcta
     1981 gagggaattc gatcccactg tagcggattg tgggtacagg gcaccgctac ctccccatct
     2041 agcacggcaa atttgctaac tcaattgcga tgttctatag tattttacta tatttgatac
     2101 cttaaatcaa gctgcaaatt attatgtaag gcgtgtaagt tttggactgc acaaaaatat
     2161 tatggttatt ttttcttttg cctgttacaa ttttttctaa tcggctaggg gtgtaacttt
     2221 acaggttctc aaataaatga tattatgaat atataggctt atctaatttg tttgagaagt
     2281 cttttttata gtttcattaa aagctcacct gtataaatac acatttttgt gtgataaata
     2341 ttaaaaaatt acaaaggagg ggttcatata tatacgatcg gtttaattgt aagtaaaatt
     2401 agctcaaata tgcacagtat cgaggatatt aaagcagaag agcagagaag aataactgca
     2461 aatgagattg aggaaattct ctccaagaag tacaaagtca aaaggattgt cgccgacaaa
     2521 aatttaattg acaacctttt aaaaagcaaa gtcaatttcg tatttaatct aagcacaggg
     2581 atcaagggtg agagcagaca gtctcaagta cctgccttgt tggaaatgct aggaatacct
     2641 tatgtaggtt ccggtgtgct ggcccatgct ttagcactaa ataaggcatt tgcaaaaaaa
     2701 atctttaagt ttcacaatgt gcccacacca tattttcagg aattttacac gggcaaagaa
     2761 gacttagatc ccaatttgaa atttccggca atagtaaaac cagcctgtga aggttccggt
     2821 tttggtatcc acaaagacaa tttggttaac acggacaaag acctgtacaa aagagttgaa
     2881 aggttattaa agcactatca gcccccggca atagtcgaag aatatattga tggcagggaa
     2941 tataccgtag gtataatcgg caatggcaat gacaaaacgg tattgcccat tgtagaaatt
     3001 gactttgata aggtcactgc agaatttgac aagtttaaca ccttcgaatc taaacatggc
     3061 caagggggac aaaagcaaat cctttgtcct gccaaattga ctgaagaaca gaa
//
LOCUS       DAONUO010000252         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21486_length_3112_cov_3.030749, whole genome shotgun sequence.
ACCESSION   DAONUO010000252 DAONUO010000000
VERSION     DAONUO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21486_length_3112_cov_3.030749"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1506)
                     /gene="hisS"
                     /locus_tag="PK768_08650"
     CDS             complement(190..1506)
                     /gene="hisS"
                     /locus_tag="PK768_08650"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQA61079.1"
                     /translation="MSKNIVKPSTLPGFMELLPKDQILFNKIKDTIRKTYESFGFIPI
                     DTPLIERSEILLAKGGGETEKQIYRFTKGDTDMALRFDLTVPLARYVAQHFPNLTFPY
                     RRYHIGKVYRGEKSQRGRFREFYQCDVDIIGNGKLSVVYDAEIPSIIYATFRNLGFED
                     FTVKINNRKVLNGFFASLNISSTADVLRSIDKLEKIGDRGVRAELKELGLPESIVDRI
                     LRFIKIQGSDGEKLEALKALDIANEIFREGIEELETVIEYVKSFGVPEKNYMIDLTIA
                     RGLDYYTGTVYETFLDDYPEIGSVCSGGRYDNLAEFYTTQKLPGVGISIGLTRLFYQL
                     NEAGLLEGDAPSTLTKVLVVPMDETCNEYSIKVANILREEGIVSEVHFEEAKVGKKLN
                     YANKLDIPYVILIGEDEIRRQMVSLKDMKTGDQELITIEEAIKTVK"
     gene            complement(1548..>3112)
                     /locus_tag="PK768_08655"
     CDS             complement(1548..>3112)
                     /locus_tag="PK768_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HQA61080.1"
                     /translation="DALFKAYEDVKDKADFIVIQTGDTYRLNQYMSISHERLEQVKAE
                     IFEDIDEFLGRIVENADKDMLLLLVVPFPSNEDIAKGERLTPVIVSHPSIPRGILTSA
                     TTKRDGIITNTDLAAQVLGYFGIDKPSIMTGHHLTSKLIEEPLEYLKNLNKISVFNYN
                     TRAIVVKTYIAFIIVILLFTLILMVYFKQYVKWMKPLLIAVLIVPTALLILPLFNPWN
                     TIKFVFCLIMTVTAYSVGIFYLLKDNMKIFVSSCLLSTGIILVDTFLSNPLMRVSVLG
                     YDPIIGARFYGIGNEYMGYLLGTTLIGTAALIDMYRGKASLPKLVSIGIYAVVLITLM
                     LPILGTNVGGTMAAFLGFGAATLLHLKGNISKKDLIVLCCLLLIFLFALFVYDSLRPS
                     ERQSHIGQTSSLIRQNSIFALFQIFARKLATNYKLVRYSTWTLVLIATMVTLAVLFRW
                     PVGILKKIFRKHNYLYFGFISGIIGTVAAFVFNDSGVVAAAMAMIPITVSLIMMCIDE
                     DLQTSLSTDKNN"
BASE COUNT          849 a          699 c          590 g          974 t
ORIGIN      
        1 ggcccggcat ggctagcatg tttcttgcat tgatgatctt ttgaaactca aattcggttt
       61 tgattttgcc gatttgggaa attttatttc cgctaatggc tatatcggct tcttttgtaa
      121 gcagcttacc atcttcaaac accagcacct tggcgttttt gatcaatata ttcacgctgt
      181 gtcctccttt cacttaacag tttttatggc ctcttctatg gtaataagtt cctggtcacc
      241 tgttttcatg tccttgagcg acaccatttg tcttcttatt tcatcttccc ctataagtat
      301 gacatagggg atatccagtt tgttggcata atttagcttt ttacccactt ttgcttcttc
      361 aaagtgcact tcggagacaa tgccctcttc cctaagaata tttgcaactt ttatggaata
      421 ttcattgcat gtctcatcca tgggcaccac taggaccttt gtcaaagtgg agggggcatc
      481 accttctagc aggcccgctt catttagctg gtaaaacagg cgggtgagac caatggaaat
      541 tccaacaccg ggcagttttt gagttgtata aaattctgcc agattatcgt accttccacc
      601 tgagcatacg ctacctattt cgggataatc gtcaaggaaa gtttcgtata cggtcccagt
      661 gtagtaatcc agtcccctgg caatagttag gtctatcatg tagtttttct ccggtacgcc
      721 gaagcttttg acatactcga ttacagtttc cagctcctcg atgccttccc ggaatatttc
      781 gtttgcaatg tcaagggctt tcagagcttc gagcttttcc ccatcgcttc cctggatttt
      841 tataaaacgt aaaattctgt cgacaatgct ttccggcaat ccaagctctt ttaattcggc
      901 ccttacaccc ctatccccta ttttctccag cttgtcaatg ctccttaata catctgcagt
      961 gcttgaaatg ttcaacgaag caaaaaagcc gtttaatacc ttcctgttat ttatcttgac
     1021 cgtaaaatcc tcaaagccca ggttcctgaa tgtagcgtat attatgctcg gaatttctgc
     1081 atcatagaca acgctgagct ttccattgcc gataatgtcc acatcgcact gataaaattc
     1141 cctaaatctt ccccgctggc ttttttcgcc cctgtaaacc ttacctatgt ggtacctgcg
     1201 atatgggaag gtgagatttg gaaagtgctg ggccacgtac cttgccaagg gcaccgtaag
     1261 gtcaaacctc agggccatgt cagtatctcc ctttgtaaag cggtaaatct gcttttccgt
     1321 ttcgcctccc cctttggcaa ggagaatctc tgacctctcg atgagggggg tatcaatggg
     1381 aataaatccg aaactctcat aagtcttcct gattgtatcc ttgattttgt taaaaagtat
     1441 ctgatctttg ggcaataatt ccataaagcc cggcaatgtc gatggcttta caatattttt
     1501 actcatttca acctccactt ttttcaacta ttaatttgta cagtttttta attgttttta
     1561 tctgttgata aactcgtctg taaatcctcg tcgatacaca tcattatcaa agaaacggta
     1621 atgggtatca tggccatggc agcagctact actccagaat cattgaagac aaatgctgcc
     1681 actgtcccta ttatacctga tataaatcca aaataaaggt aattgtgctt gcggaaaatc
     1741 ttctttagta ttcccacagg ccagcgaaag agtacagcta gagttaccat cgtagcgata
     1801 aggactaagg tccatgtgga gtatcttacc agtttataat tggtggcaag ctttcttgca
     1861 aatatctgaa agagagcaaa aatactattt tgtctgataa gggagctggt ttgacctata
     1921 tgggattgcc tttcagaagg tcgcaaacta tcataaacaa atagggcgaa taagaatatc
     1981 aataagaggc agcaaagaac aatcaaatct tttttgctta tattaccttt taaatgcaag
     2041 agtgtcgcgg caccgaagcc taaaaaggca gccattgtcc cgcccacatt tgtgccaagg
     2101 ataggcagca tcaatgttat cagaactacg gcatatatgc ctatacttac aagttttggc
     2161 aagcttgcct tgcctctgta catatctatt aatgcagcag taccgataag ggttgtaccc
     2221 agtaaatagc ccatgtattc gttcccaatg ccgtaaaacc ttgctcctat gatgggatcg
     2281 tagcccaaaa ctgaaaccct catcagcgga ttacttagaa atgtatccac cagtataatt
     2341 cctgtagaca ataaacagct ggatacaaat atcttcatat tgtcttttag caggtaaaaa
     2401 atgcccacgc tatatgcggt tacggtcata attaagcaaa agacaaactt tatggtattc
     2461 cagggattaa acaagggcaa aatcagcaat gccgtcggca caatcagcac tgctataagc
     2521 aatggtttca tccatttgac gtattgtttg aaatatacca tcaggattaa agtaaatagc
     2581 aaaatcacga taataaaagc aatataagtt ttgactacaa tcgccctcgt attataatta
     2641 aacactgata ttttattgag gtttttcagg tactccaggg gttcctctat cagctttgaa
     2701 gtcaaatgat gacccgtcat tatggatggt ttgtcgatgc caaaatatcc gagcacctgg
     2761 gctgctaagt cggtgtttgt aattattcca tctctcttgg tagttgccga agtcaatatc
     2821 ccccttggaa tagaaggatg cgaaactatc acgggcgtta gcctctcgcc ttttgcaata
     2881 tcttcatttg agggaaaggg aacgaccaat aacaacagca tatctttgtc ggcattttca
     2941 acgattctcc ctaaaaactc atcgatatct tcaaatatct ctgcctttac ttgttcaagt
     3001 ctttcatggg agatgctcat gtattggttc agcctgtacg tgtctccggt ctggatgacg
     3061 ataaagtcag ccttgtcctt tacgtcttca taagctttaa atagagcatc gt
//
LOCUS       DAONUO010000253         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21542_length_3106_cov_4.144543, whole genome shotgun sequence.
ACCESSION   DAONUO010000253 DAONUO010000000
VERSION     DAONUO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21542_length_3106_cov_4.144543"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..208
                     /locus_tag="PK768_08660"
     CDS             2..208
                     /locus_tag="PK768_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HQA61081.1"
                     /translation="MGFVSDFDIDLERGVIKSIIIPGQSKLFSIFSKTGDYVIPWEQI
                     KTIGSDVILVELQHYIHSNKAGTP"
     gene            267..722
                     /gene="nrdR"
                     /locus_tag="PK768_08665"
     CDS             267..722
                     /gene="nrdR"
                     /locus_tag="PK768_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQA61082.1"
                     /translation="MKCPFCGNMESKVMDSRPTEEGTIIRRRRECPSCKKRFTTYERV
                     DELPLTVIKKSGIREVFDTHKIINGLIKACEKRPISIEMMQEIANDIEKELKNNMELE
                     VESKKIGEMVMDRLKELDEVAYVRFASVYRQFKDIDSYIEEIERLKSNR"
     gene            738..2450
                     /locus_tag="PK768_08670"
     CDS             738..2450
                     /locus_tag="PK768_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQA61083.1"
                     /translation="MAVKNKLDMKELLSAEVFLLPVKTFGSVPINISLVYPNTYSMGM
                     SNLGFHSIYYQINSRDDALCHRAFIPSYENADNITTLEGDKSINEYDIVGFSISFELD
                     YINIIKILESANISAFSQNRNGPLVMAGGPAATFNPEPLSPFVDFFVIGEGEEVIHDI
                     LEQYRHFQNRDKQEILEALAQIKGVYVPSFYKVAYDKEGKVLDIRHESYVPAKVTKRW
                     IKDIDEYNTESVILTPYTEFKDMFLTEVSRGCGRNCRFCMAGYCYRIPRHRSLDKVLE
                     RAEFGSKHKDKIGLVGAAVSDYPFIDQLAEQLVENNIKFSVSSLRADTLREPLMQGLA
                     FSGHKTLTIAPEAGSDRLRQVINKGITDEHVINSVKLAHKYRVDNLKLYYIIGLPGES
                     DEDLKEMVDFLLSLKEYMKAIGNKRGMLTVSVNPFIPKPFTPFQWLGMEPVKLLNDRI
                     NFLKTQLRPRGIKLIFESPRLSEIQSALARGDRQTGLLLYDIYKKGASTTAFKKAEIE
                     GKSIDFYAHRPLDQDEILPWEHIDIGLKKEFFISEFHRAMEGKPTTRCTDEKCASCKI
                     CHTK"
     gene            2465..>3106
                     /locus_tag="PK768_08675"
     CDS             2465..>3106
                     /locus_tag="PK768_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HQA61084.1"
                     /translation="MAFKLNYQGGLAYLTIPAFEKTGLVKHGFSTRMGGMSKHPYDSL
                     NLGLRKDDDKKNVIENYRLFCKALSINPNNLVASDQVHEDKIHVATLHDRGKGIFKQS
                     DIKGIDALITKDRQLALVTYYADCVPLFLLDVETPAIGLAHAGWRGTVKKIGQKTVLK
                     MMKEFGSKPEHILVGIGPCISVCCYEVDELVVDSLKKAFVYFDELIKETGKGKW"
BASE COUNT         1019 a          557 c          706 g          824 t
ORIGIN      
        1 actgggattt gtttctgatt ttgatataga tttggaaaga ggagttataa aatctataat
       61 catccccggt caaagcaagc tgtttagcat tttttctaag acgggagatt atgtaattcc
      121 ctgggaacaa atcaaaacta taggctccga tgttatcctg gtcgaactgc aacattacat
      181 ccattcaaat aaagcaggaa caccttgaat ttctttaaat ttacagcgaa aacaagtata
      241 atatctatat gggaggtggt ggtagaatga aatgcccctt ttgtggcaat atggaaagca
      301 aggttatgga ttctcggcca acggaggaag ggacaataat cagaaggcgt cgtgaatgcc
      361 cttcatgcaa aaagagattt accacctacg aaagggtgga cgagctgccc ttgacggtta
      421 taaagaagag cggtatacgt gaggtttttg acacgcataa aataataaac ggcctgatta
      481 aagcctgcga gaaacgtcca atttctattg aaatgatgca ggagatcgcc aatgacattg
      541 agaaggaact gaaaaataac atggaacttg aagtggaaag caaaaaaatc ggtgaaatgg
      601 tgatggacag actcaaggaa ttagacgagg tggcatatgt aagattcgca tcggtttaca
      661 ggcaattcaa agatatcgat tcttacatcg aagaaattga aagattgaaa agcaacagat
      721 aaatattgga ggaaataatg gctgtaaaga acaaactgga tatgaaagag cttttatcag
      781 ctgaggtatt tttattaccc gttaaaactt ttggctctgt acctataaat atatcattgg
      841 tctatcccaa tacatacagt atgggaatgt caaacctggg atttcattcg atatattatc
      901 agataaattc cagggatgat gcactctgcc atcgagcctt cattccaagt tacgagaatg
      961 cagacaatat tactactctt gaaggggaca aatctataaa tgaatatgac attgtaggat
     1021 tttccatatc ctttgagctg gattatatca acataattaa aatcctcgag tctgctaaca
     1081 tttccgcttt ttcacaaaac agaaacggac ccttggtaat ggcaggagga cctgcagcca
     1141 ccttcaatcc cgaacccctt tcaccttttg tggatttttt tgtcatcggc gagggtgaag
     1201 aagtgataca tgatatactg gagcagtaca ggcatttcca aaacagggac aagcaggaaa
     1261 ttcttgaggc actagcgcaa attaagggag tgtatgttcc ttcattttac aaagtagcat
     1321 atgacaaaga gggaaaagtc ctggatataa gacacgaaag ctatgtacct gctaaagtca
     1381 ctaagagatg gataaaagac atagacgagt acaatacgga atccgttatc ttaacaccct
     1441 atacggaatt taaagatatg tttctgacag aagtgtcaag gggctgcgga agaaattgta
     1501 ggttctgcat ggcgggttat tgttatagaa ttccgcgtca caggtcattg gataaggttt
     1561 tagagcgggc cgagtttgga tcaaaacaca aggacaaaat cggccttgtc ggagctgccg
     1621 tatccgatta tccttttatt gaccaattgg ctgagcagtt ggtggaaaac aacataaagt
     1681 tttcggtgtc atccctgcgt gccgacacct tgagagagcc cctgatgcag ggattagctt
     1741 tcagtggtca caagacctta actattgccc cggaagccgg ttccgataga ctaagacagg
     1801 tcataaataa gggcataacc gatgagcatg tgataaactc ggttaagctg gcacacaagt
     1861 atagagttga caatttaaaa ctgtactaca tcatcggatt acccggggaa agcgatgaag
     1921 atttaaagga aatggtggat tttttattat ctttaaagga atacatgaaa gccataggga
     1981 acaagagggg aatgttgacc gtaagcgtaa atccctttat tcccaagcct tttactcctt
     2041 ttcagtggct cggaatggag cctgtaaaat tgctaaacga cagaatcaat ttcctaaaaa
     2101 ctcaactaag acctaggggc ataaagctaa tatttgaaag tccgcgactt tcagagattc
     2161 aatcggctct cgcccgaggc gataggcaga ctggcctttt gctctatgat atttacaaaa
     2221 aaggtgccag cacaactgca tttaaaaagg ctgaaattga aggcaaaagt atagactttt
     2281 acgctcatcg cccattagac caggatgaaa tcttgccttg ggaacatatc gacataggtt
     2341 taaaaaagga attttttatt tccgaatttc atcgagcaat ggaaggcaaa cctacgacaa
     2401 ggtgtaccga cgaaaaatgc gcgagttgca aaatttgcca tactaaataa atggggtgca
     2461 tgtaatggct tttaaactaa attatcaagg gggcctggct tatcttacaa tacccgcttt
     2521 tgagaagacg ggtcttgtga aacacggttt ttctacccgc atgggaggta tgagcaagca
     2581 tccgtatgac agcttgaatt tgggtttgag aaaagatgat gacaagaaaa atgtcataga
     2641 aaactacagg cttttctgta aggctctgag cattaacccc aataacctag tagcatcgga
     2701 tcaggttcat gaagataaaa tccatgtagc aaccttacat gataggggaa agggaatttt
     2761 caagcaatcc gatatcaaag gcatagatgc acttataact aaagataggc agctggcctt
     2821 agtaacctat tatgcggact gcgtaccgct gtttttatta gatgttgaaa ctcctgctat
     2881 tggtcttgca cacgctggct ggcgaggaac tgtgaaaaag attgggcaaa agactgtact
     2941 taagatgatg aaagaatttg gctcaaagcc tgaacacatt ttagttggca taggtccctg
     3001 cataagtgtc tgctgctatg aagtggatga actagttgta gatagtctaa aaaaagcctt
     3061 tgtttatttt gatgagctca tcaaggagac aggtaaaggc aagtgg
//
LOCUS       DAONUO010000254         3098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21611_length_3098_cov_3.633585, whole genome shotgun sequence.
ACCESSION   DAONUO010000254 DAONUO010000000
VERSION     DAONUO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21611_length_3098_cov_3.633585"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PK768_08680"
     CDS             <1..493
                     /locus_tag="PK768_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HQA61085.1"
                     /translation="LTVRERKGTLSGLKVAIIGDILHSRVARSNIFGFTKMGSEVYVA
                     GPSTLMLPDIEQTGAKLAETIEEAVKDADVVIALRIQLERQQKALFPTIREYARLFGL
                     NRDKLALARPDALLLHPGPVNRGIELTSEIIDGPQSVINQQVRSGVAVRMSVLYHLIG
                     GSR"
     gene            490..1770
                     /locus_tag="PK768_08685"
     CDS             490..1770
                     /locus_tag="PK768_08685"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQA61086.1"
                     /translation="MMKTLLKGARLLDPGQNFDAIMDILIVDGKIAEIKPDINISDTE
                     IIDLSGKLATPGLIDMHVHFRDPGYEYKEDIESGSMSAAAGGFTAVACMPNTNPPLDN
                     SSLIEYVKSKASKVGKVKVLPIGCVTKGQKGEEITEMGDMARARAVAFSDDGKPVASA
                     SLMRKAMVYAAMFDRLIIDHCEDPSLFEGGQINEGRVSTLLGLMGIPSACEEIMVARD
                     ILLAREMKTPVHIAHISTRGSVELVRWAKREGVKVSCEVTPHHLTLTEEAVMDYDTNA
                     KVNPPLRTKEDLEALLEGLKDGTVDAIATDHAPHHIDEKDLECDKAAFGLIGLETALG
                     VILTKVVGENGIPLNTVIEKMTVGPAKVLGLDMGTLKVGGPADITVIDLEREWTVDKD
                     KFFSKGRNTPFHGFKLKGKAVMTIVDGKSAYSEM"
     gene            1890..>3098
                     /gene="carB"
                     /locus_tag="PK768_08690"
     CDS             1890..>3098
                     /gene="carB"
                     /locus_tag="PK768_08690"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQA61087.1"
                     /translation="MPKDKTIKKIMVIGSGPIVIGQAAEFDYAGTQACLSLREEGYEV
                     ILINSNPATIMTDSHIADKIYIEPLTLEFAAKILRRERPDGLLPTLGGQVGLNLAVEL
                     AEKGILHREGVKLLGTPLTSIKKAEDRELFKKLMEEINEPVPDSRIVHSLEEARAFAR
                     EVGLPLIVRPAYTLGGTGGGVANTEEELMEITYNGLRLSRIHQVLLEKSLLGLKEVEF
                     EIIRDRKDQCICVCDMENIDPVGIHTGDSVVVAPSQTLTKDEYEKLKRASFKIIRALG
                     IEGGCNVQFALDSSTGKYYVIEVNPRVSRSSALASKAAGYPIAKVTAKIATGLALDEI
                     KNPVNGVGSAYYEPSLDYVVVKMPRWPFDKFVDADRFLGTQMKATGEVMAIDKNLEGA
                     LMKAARSLEQG"
BASE COUNT          873 a          761 c          847 g          617 t
ORIGIN      
        1 gcttacggtg agggaaagga aggggaccct gtcgggcctg aaagttgcca taataggcga
       61 tatacttcac agccgcgtgg ccaggtcaaa catcttcggc tttacgaaaa tgggctcgga
      121 agtttatgtg gctggcccca gcaccctgat gctcccggac attgaacaga cgggagcaaa
      181 gctggccgag acaattgaag aggccgtcaa agatgcagac gtagtaatcg cccttcggat
      241 tcagctggaa cggcagcaga aggccctttt ccccactata agggagtacg ccaggctttt
      301 cggcctaaac agggacaagc tggccctggc caggcccgac gccctactgc tgcatcccgg
      361 ccccgtcaat cgtggaatcg agcttacatc tgaaataatc gacgggcccc aatccgtcat
      421 caaccagcag gtgagaagcg gcgtcgccgt gcgcatgtcg gtactatacc atctgatagg
      481 aggaagccga tgatgaaaac acttttaaag ggagcaaggc ttttagaccc cggccaaaat
      541 tttgatgcca taatggacat tctaatagta gatggtaaaa tcgcagagat taagccggat
      601 ataaatataa gcgatacaga gataatcgac ctttcgggaa agctagcaac tcccggtcta
      661 atagatatgc acgttcactt tagagacccc ggctacgaat acaaggaaga tatagagtcg
      721 ggcagcatga gcgccgctgc cggaggcttt accgcggtgg cctgcatgcc caacaccaac
      781 ccgcccttag acaacagttc ccttatcgaa tacgtgaaat ccaaagcttc aaaggtggga
      841 aaggtaaaag tcctccccat aggctgcgtg accaagggcc aaaagggtga ggagattacc
      901 gagatgggag acatggccag ggcacgagcg gtagcctttt ccgacgacgg taagcccgtg
      961 gcaagtgcct ccttgatgcg aaaggccatg gtctatgcgg ccatgttcga caggctgatc
     1021 atcgaccact gtgaagatcc cagccttttt gaagggggcc agatcaacga aggccgcgta
     1081 tccaccctgc tgggcctcat gggaataccc agtgcctgtg aagagataat ggtggctagg
     1141 gacatcttgc tggccagaga aatgaaaacc cccgttcaca tagcccacat aagcacaagg
     1201 ggttcagtgg aacttgtgcg atgggcaaag cgggaaggag taaaggtttc ctgtgaagtt
     1261 acaccgcacc atcttaccct caccgaggaa gcggtaatgg actatgacac caatgcaaaa
     1321 gtaaatccgc cccttcggac gaaagaggac ctagaggcac tcctggaagg cttaaaggac
     1381 ggcaccgtag atgccatcgc cacagaccat gcgccccacc acatagacga aaaagacctg
     1441 gaatgtgaca aggcggcttt tggcctgata ggccttgaaa ctgcccttgg agttatcttg
     1501 acaaaagtag taggggaaaa tggcattccc ttaaacacgg tcatcgaaaa aatgacggtg
     1561 ggtcctgcca aagtattagg ccttgacatg ggaacactta aggtaggagg acccgcagat
     1621 attaccgtaa tagatttgga gcgggaatgg acggtggata aagacaaatt tttctccaaa
     1681 ggccgaaaca ctcctttcca cggttttaaa ttaaaaggca aggcggtcat gaccatagtg
     1741 gacggcaaaa gtgcgtattc ggaaatgtga tgaataaata tacaaaaata tgtattgata
     1801 tacggaaaat atcgtgttaa aatacaatat aatgaataaa tatacgaata cgattttagc
     1861 gcatgggcct ttaggaaggg agatttgtta tgcctaaaga taagactatc aaaaaaatca
     1921 tggttatcgg ttcggggccg attgtaatcg gccaggcggc agaattcgac tatgccggca
     1981 cccaggcctg cctgtccctc agggaagaag gctatgaggt aatcttaatt aacagcaatc
     2041 ctgctaccat catgaccgat tcacatatag cggacaaaat atatatagag cccctgaccc
     2101 tggaatttgc agccaagatc ttgcgccggg aaaggcctga cggccttttg cccaccttgg
     2161 ggggccaggt aggcttaaac ttggccgtgg aactggcgga aaagggcatc ctgcacaggg
     2221 aaggcgtaaa gctcttgggg acgcccttaa cctccataaa aaaagcggag gaccgggaac
     2281 tctttaaaaa actcatggag gaaatcaacg aaccggtgcc cgacagccgg atagttcaca
     2341 gcctggaaga ggccagggcc tttgccaggg aagtgggact gcctttaatc gtccggcctg
     2401 cctatactct tggtggcacc ggcggcggcg tggcaaatac agaggaagaa ctgatggaga
     2461 taacctacaa cggcctaagg ctcagccgca tccaccaggt cctactcgaa aagagcctct
     2521 tgggcttgaa ggaagttgag tttgagatca ttcgggaccg aaaggaccag tgcatatgcg
     2581 tctgcgacat ggaaaacatc gaccctgttg gaattcacac gggggacagc gtggtcgtag
     2641 caccatccca gaccctgacc aaggatgaat acgagaagct gaaaagagca tcctttaaaa
     2701 taatccgggc cctcggcatt gaagggggct gcaacgtaca gtttgccctg gattcctcta
     2761 cgggcaagta ctatgtcatc gaagtaaatc cgcgggtgag ccgttccagt gccttggcct
     2821 ccaaggctgc cggctacccc atcgcaaagg ttaccgccaa aatcgcaacg ggcctggccc
     2881 tggatgagat aaaaaatccc gtcaacggcg tcggcagcgc ctactacgag ccgtcgcttg
     2941 actacgtagt cgtaaagatg ccccgctggc cctttgacaa gtttgtggac gccgacaggt
     3001 ttttaggcac tcagatgaag gccaccgggg aggtcatggc cattgacaaa aatctcgagg
     3061 gggccctcat gaaggcggcc aggtctttag aacaggga
//
LOCUS       DAONUO010000255         3092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21672_length_3092_cov_3.168917, whole genome shotgun sequence.
ACCESSION   DAONUO010000255 DAONUO010000000
VERSION     DAONUO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21672_length_3092_cov_3.168917"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /gene="fba"
                     /locus_tag="PK768_08695"
     CDS             <1..873
                     /gene="fba"
                     /locus_tag="PK768_08695"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HQA61088.1"
                     /translation="IGAFNVNNMEIIQAIVEAAKEEKSPLILQVSAGARNYAKPVYLR
                     KLVEAAIEDTDLPIVLHLDHGEDFEICKACIDDGFTSVMIDGSKYPFEENIRLTKQVV
                     DYAHERGVVVEAELGRLAGIEDAVKVSEKDAILTDPDEAVEFVERTGVDSLAIAIGTS
                     HGAYKFKGEPKLDFERLDKITELLPGFPIVLHGASSVPSELVELCNKYGGQIPGAQGV
                     PEDMLRKAATMGVCKINIDTDLRLAMTASVRKYLVENPSEFDPRKYLGAGREAIKQLV
                     KHKIRNVLGSSGKA"
     gene            1133..2659
                     /locus_tag="PK768_08700"
     CDS             1133..2659
                     /locus_tag="PK768_08700"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQA61089.1"
                     /translation="MKRILVIILITALFFSIFVLIKNFFAATLATTDAEFVEEQKEYQ
                     KDYSVPERHVYHIKVNYEGKNQLRVQMDLTYVNNTGSTLEELYFHLYPNMFSSPERVP
                     FFRQDFYRAFPQGYSFGALDIIEVKQDGREVAWSLIQEDQILQLALDNPLHPEEISTI
                     FMEFQLTVPQARFRFGYQEFGKDKITVSLGNWYPIPAVFKDGRWNLDRHQAVGDCTYS
                     DIADYTVSFTVPKGFTVAASGVLEDNYTKGSMTVFEYSIDKIREFGAAISNNYQTASE
                     KVDGIKIISYFHPEDKRGGFMALNVAKYALKIFNEAFGKYPYPDLRIAEANYYPGGME
                     FPTFIMMDSGRYQEASLRNSSLERSTAHEVAHQWWYNLVGNDQINEPWLDEGITEFAT
                     AYYFEKRYGTPGRESYYSRQVDTTIDFIKNNPRHMLDPLPDFTSHGEYFAEVYVSGVL
                     FYEDLRIRIGEDELLEFLRSYLETFKYKNVSLQEFIEFLKGKNYEALDESFYDKWFNH
                     "
     gene            complement(2710..>3092)
                     /locus_tag="PK768_08705"
     CDS             complement(2710..>3092)
                     /locus_tag="PK768_08705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQA61090.1"
                     /translation="EKYGVVVNLSQQKVYIYEDNVLVKTFACSSGLDENYYYTPKGLY
                     RIGERGDSFFSPKYGQGAYHWVRFNNNYPFHSVPFDANRNIIEEEAAKLGQKASHGCI
                     RLSLDDALWFYNNIPKGTPVLIKD"
BASE COUNT          892 a          642 c          709 g          849 t
ORIGIN      
        1 ataggtgcct tcaacgtcaa caatatggaa atcattcagg ctatcgttga ggcagccaag
       61 gaagagaagt ctcctctcat attgcaggtg tcggcaggtg caaggaatta cgcaaaacca
      121 gtttatttaa ggaaattggt tgaagcggct attgaagaca cggatctgcc aatagtcctt
      181 caccttgacc atggtgaaga ctttgaaata tgcaaagctt gtattgacga cggcttcact
      241 tcggtcatga tcgatggttc aaaatatccc tttgaggaaa acattaggct caccaaacag
      301 gtggtagatt atgcccatga gcggggtgtc gtagtagaag ccgaactggg tcgactggca
      361 ggtatcgaag atgctgtaaa agtatccgaa aaggatgcta tcttaaccga ccctgacgaa
      421 gccgtggaat ttgtagagag aaccggcgtt gactcattgg ccattgctat aggaacgagt
      481 cacggagctt ataagtttaa aggcgagccg aaacttgatt tcgagcgttt ggataagata
      541 accgagttgc tgcccggatt tccaatcgtg ctccacggtg cctcttcagt tccaagcgaa
      601 cttgtagagc tgtgcaataa gtacggaggc cagattcccg gtgctcaagg tgttcccgag
      661 gatatgctaa gaaaagctgc taccatgggc gtatgcaaga ttaacataga cacggacctc
      721 cgccttgcga tgaccgcatc ggtgagaaaa tacctggtgg aaaacccgtc ggaatttgac
      781 ccgcggaagt acctgggagc aggccgggaa gccataaaac agttggtaaa acacaagata
      841 agaaacgtat tggggtcaag tggaaaggca taaatgatag aaaggaaatg ccttgatacc
      901 tttaaagctc gcattttttg cgagcttttt tatatatgat tttttggccg ataacatgta
      961 actgctttac tatttcgtat tttaagcgtg tttcatattg tctaagacaa tttaattgaa
     1021 ttttattgtt acgtaaagct atgcgcccta taaaggattt atcttttttt tatagaaatg
     1081 ttatatacat gaattaaatg aaataaatgg aatagcatta ggaggatatg atttgaaaag
     1141 gattcttgta atcatcctga taaccgcatt atttttttcc atttttgtcc ttataaaaaa
     1201 cttttttgcc gccaccctgg caacaacgga tgcggaattt gtagaggagc aaaaggagta
     1261 ccaaaaggat tactcggtcc cggagcggca tgtttaccat attaaggtaa actatgaagg
     1321 taaaaaccaa ctccgggtcc aaatggactt gacctatgtg aataacaccg gaagcactct
     1381 ggaagaactt tattttcatc tctatcccaa catgttttcc agtccagagc gggtcccctt
     1441 ttttaggcag gacttttatc gtgcctttcc ccagggctac agctttggag ccctagatat
     1501 aatcgaagta aaacaggacg gcagagaagt tgcttggtct ttaatacagg aagaccaaat
     1561 ccttcagcta gctttagata atcctttaca tccggaagaa atatcgacta tcttcatgga
     1621 gtttcagctc acagtccccc aagctagatt ccgttttgga taccaggaat tcggcaagga
     1681 caaaattacc gtatctctag gcaattggta tcccatcccg gctgttttca aagatggacg
     1741 gtggaacctt gacaggcacc aggctgtggg tgactgcacc tatagcgata ttgctgacta
     1801 caccgtaagc tttacggtcc cgaagggctt taccgttgca gcttcagggg tcttagagga
     1861 taattatacg aagggcagca tgaccgtatt tgagtactcc atcgacaaaa tacgcgaatt
     1921 tggagcagcc atcagcaaca attatcaaac tgccagtgaa aaagtagacg gaattaagat
     1981 catatcctac tttcaccctg aagacaagcg cggcggcttt atggccttga acgtggccaa
     2041 atatgccctg aaaattttca atgaagcctt cggcaaatat ccctaccctg atcttagaat
     2101 tgccgaggcc aactactatc ccggcggcat ggagtttcct acttttatca tgatggactc
     2161 tggcagatac caggaagcca gcctccgaaa tagttccctt gaaaggtcta ctgcccatga
     2221 agtagcacat caatggtggt acaatttggt gggaaatgac cagataaacg aaccctggct
     2281 cgatgaaggg ataaccgagt ttgccaccgc gtactatttt gaaaagcgtt acggaacgcc
     2341 gggaagagag tcttactaca gcaggcaggt agataccacc atagatttta taaaaaacaa
     2401 tccccgacac atgctggacc ctctccctga ttttacaagt cacggcgagt actttgcgga
     2461 agtctacgta tccggtgtgt tgttttatga agacctgcgc attagaatag gtgaagatga
     2521 gcttcttgag tttttaaggt catatttgga gacatttaag tacaagaatg tcagtttgca
     2581 ggagtttata gaatttttaa agggcaaaaa ttatgaagct ctcgatgaga gcttctacga
     2641 taaatggttt aatcattaac ttttcaaaga tacttatatt tgtgacggaa tacaattggt
     2701 tattaggatt taatccttaa tcaataccgg ggtgcccttg ggtatgttgt tgtaaaacca
     2761 cagagcatca tccaaggaca ggcgtataca tccatgtgag gctttttgtc ccagtttggc
     2821 cgcttcctct tcaataatgt tcctatttgc gtcaaagggg acgctgtgga agggatagtt
     2881 gttgttaaac ctcacccaat gataggcccc ttgtccgtat ttcgggctga aaaaggagtc
     2941 gccgcgttcg cctatcctgt acagcccctt tggtgtgtaa tagtagtttt catcaaggcc
     3001 cgaggaacat gcaaatgtct tgaccaaaac attatcctca tatatgtata ccttctgctg
     3061 gctgagattt actacaaccc cgtatttttc at
//
LOCUS       DAONUO010000256         3079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21780_length_3079_cov_3.434854, whole genome shotgun sequence.
ACCESSION   DAONUO010000256 DAONUO010000000
VERSION     DAONUO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21780_length_3079_cov_3.434854"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="spoVAD"
                     /locus_tag="PK768_08710"
     CDS             complement(<1..890)
                     /gene="spoVAD"
                     /locus_tag="PK768_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HQA61091.1"
                     /translation="MKKLGRQTVQFEKAPSIIGTATIVGPKEGQGRFKDYYDLILKDS
                     IYQERSWEKAETKILKESVEMAIKNSKVPTAKIDYFIAGDLLNQIISAGFAARELGLP
                     FLGIYGACSTIAQGAIIGAMIIDGDFGENVVAAVSSHFCTAERQFRFPLELGNQRKPT
                     SQWTVTGAGAMVLSAKAHNPRITRATVGKVIDMGEADPNDMGTAMAPAAVDTIVRHFE
                     DTGKTPDDYDLILTGDLGSIGKDIAEELLRQKGYDISNKYTDCGVLIFYPEQDVHAGA
                     SGCACAAVVTCGYVYKEMIRG"
     gene            complement(928..1380)
                     /gene="spoVAC"
                     /locus_tag="PK768_08715"
     CDS             complement(928..1380)
                     /gene="spoVAC"
                     /locus_tag="PK768_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HQA61092.1"
                     /translation="MPVLTKEQQDYKKFSKSKRPKPPLLKNMTMAFVVGGTICVIGQV
                     FLSFFIAKGLTPSQAAGPTSIIMIFFGAFLTGTGVYDKLGKYAGAGSIVPITGFANSI
                     VSPAMEFKREGYVFGVAAKMFVVAGPVLVYGITSATIIGLIYYLLHML"
     gene            complement(1438..1644)
                     /locus_tag="PK768_08720"
     CDS             complement(1438..1644)
                     /locus_tag="PK768_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQA61093.1"
                     /translation="MTVKVIELVGESKSGWQEAVQNAVKDASKTIRNITGVEVLNNTA
                     NVKDGNIVEYKANVKIAFKVDGTS"
     gene            complement(1917..2645)
                     /gene="sigF"
                     /locus_tag="PK768_08725"
     CDS             complement(1917..2645)
                     /gene="sigF"
                     /locus_tag="PK768_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HQA61094.1"
                     /translation="MGFENTIELLKKAKRGDKEAKDKLVSLNLGLVWSIVKKFNNRGY
                     ELEDLFQVGSIGLLKAIEKFDFSYDVKFSTYAVPMIMGEIRRYIRDDAPVRVARSLKE
                     LAYKIQKTKEELTNTLEREPTIKELSAEMGVPVEELIMAMEASQSLLSLNEVMYQDDG
                     SPIYLIDQIDEKEQGQNILFDRIALKEALSKLNKNERRIIILRYFKDMTQVEVAKILN
                     MTQVQVSRLERKILVKVKDLLSQA"
     gene            complement(2651..>3079)
                     /gene="spoIIAB"
                     /locus_tag="PK768_08730"
     CDS             complement(2651..>3079)
                     /gene="spoIIAB"
                     /locus_tag="PK768_08730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HQA61095.1"
                     /translation="EMQLDFVSKSENESFARVVVAAFASQLDLTLDELADIKTAVSEA
                     VTNSIIHGYEDTSGIIRISSKLYEDRIVITVQDWGKGIEDIEKARQPLWTSKPEQERS
                     GMGFTIMEDFMDELRVESAPGEGTIVTMVKNINKGMDTAR"
BASE COUNT          824 a          725 c          496 g         1034 t
ORIGIN      
        1 cctcttatca tttctttata tacataaccg cacgtgacta cagcggcaca ggcacagccg
       61 cttgctcccg catggacatc ctgctctggg taaaatatca aaactccaca atcggtgtac
      121 ttatttgata tgtcgtagcc cttttgcctt aaaagttctt ctgctatgtc cttgcctatg
      181 cttcctaagt cacctgtcaa aattaaatca taatcgtcag gtgtcttacc tgtatcttca
      241 aaatgcctca ctatagtatc gacagccgca ggtgccattg cagtgcccat atcattcgga
      301 tcagcctcgc ccatatctat tactttaccg acagttgccc gagtgatcct gggattatgt
      361 gcttttgctg ataaaaccat tgctcctgct cctgtgaccg tccactgaga tgttggcttt
      421 ctttgattcc ccaactctaa gggaaatcta aattgtcgtt ccgcagtgca aaaatgactg
      481 gatactgctg ccacaacatt ttcgccgaaa tctccgtcta tgatcatagc gcctattatt
      541 gctccctgag caattgtgga acaagcgccg taaatgccca aaaagggcaa gcccagttca
      601 cgagcagcaa agcctgctga aatgatctgg tttaataaat ctcctgctat gaaataatct
      661 attttagctg taggcacttt gctgttttta atagccattt caactgactc ttttaagatt
      721 tttgtctctg ctttttccca actcctttct tgatagatac tgtcttttaa aatcaaatcg
      781 taataatctt tgaagcggcc ctgaccttct ttgggcccga ctatagttgc agttcctatg
      841 atagaaggag ctttttcaaa ttgtactgtt tgtctgccaa gctttttcat gcttcgaatc
      901 ttccttttag atgctaaagt gcatacacta taacatgtgt aataaatagt atattaaacc
      961 tattatggtt gcggatgtaa taccgtacac gagtacaggc cccgctacaa caaacatttt
     1021 tgctgcaaca ccgaatacat agccttctct cttaaattcc atggcaggag aaacaatgga
     1081 gttagcaaag ccggtaattg gaactatgga acctgcaccg gcatatttgc ctaatttatc
     1141 ataaactcca gtccccgtta aaaaagctcc aaaaaaaatc attatgattg aggttggtcc
     1201 tgcagcctga ctaggcgtaa gccctttggc aatgaaaaaa ctcaaaaaaa cttgaccaat
     1261 tacacagatt gttcctccca cgacaaatgc catggtcatg ttttttaaca ggggcggctt
     1321 tggccttttg ctttttgaaa acttcttata atcttgttgc tcctttgtaa gaactggcat
     1381 tgaatcaccc tgctacatta aattagttta aaacgagcac cgtctgtgcc cgttttgcta
     1441 gcttgtgcca tcaaccttga aggctatctt tacatttgcc ttgtattcta cgatatttcc
     1501 atccttgaca ttggctgtat tgtttaaaac ctcaacgccg gttatgtttc ttattgtctt
     1561 gctggcgtcc tttactgcat tttgtactgc ttcttgccaa ccacttttcg attctcccac
     1621 taattctata acctttaccg tcatatgatg tccccctact ccatacttat tgttctataa
     1681 attattctat ccaataacag aacatttatt ctgttgtaga agcactagct ttgcgctttt
     1741 tggtattctg ttattcatag tgttcttgta gaaaaaagct cctacagcga ccataaaaag
     1801 caatgcaatt attacaatca gttttctctt gatattaatc accaggatta cctcccaatg
     1861 aatattattg ttatattagc aatcttttat acctgtacaa ataaaaaaag cctaatctag
     1921 gcttgagaca ataagtcctt cactttgaca agtatctttc tttccaatct tgatacctgc
     1981 acctgagtca tgtttaaaat tttagcgacc tctacttgtg tcatgtcttt aaaatatcta
     2041 aggattataa ttctacgctc gtttttatta agttttgaca gagcttcttt caacgctatt
     2101 ctgtcaaaca aaatgttttg cccctgttcc ttttcatcaa tttggtcgat taaataaata
     2161 ggggaaccat catcttgata catcacttca tttaaagaaa gtagcgattg agatgcttcc
     2221 atggccatta taagttcttc cacaggaaca cccatttcag cggaaagctc ttttatcgta
     2281 ggttcccttt ccaacgtatt cgtaagctct tccttcgttt tttgaatttt ataagcaagc
     2341 tctttcaaag aacgagctac tcgtacgggg gcatcatcgc gtatgtacct tcgaatttca
     2401 cccattatca tagggacagc ataagtcgaa aatttgacat catatgagaa atcaaatttt
     2461 tcaattgcct tgagcaagcc gatactgcca acttgaaata aatcctccag ctcatagcct
     2521 ctgttattga atttctttac tatgctccaa accaaaccca ggttaaggga caccagctta
     2581 tcttttgcct ctttgtcacc cctttttgcc ttttttaaaa gctcaattgt attttcaaag
     2641 cccatgagtt ttacctcgcc gtgtccatgc ctttattaat gtttttgacc atcgtaacaa
     2701 tcgttccctc acccggagca gactcaaccc ttaattcatc cataaagtct tccattattg
     2761 taaaacccat tccggacctt tcttgttctg gctttgaggt ccacagtggt tgacgcgcct
     2821 tttctatatc ttcaatgcct tttccccaat cctgaactgt tatgacgatt ctatcttcgt
     2881 acaattttga actaattctt ataatgccgc ttgtatcttc atagccatga attatagaat
     2941 ttgttacagc ttcagaaacc gctgttttta tatccgccag ttcatctaaa gttagatcaa
     3001 gctgtgatgc gaaggctgct accacaaccc tagcaaagga ctcattttcg gacttgctta
     3061 caaaatccag ttgcatctc
//
LOCUS       DAONUO010000257         3069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21859_length_3069_cov_3.207034, whole genome shotgun sequence.
ACCESSION   DAONUO010000257 DAONUO010000000
VERSION     DAONUO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21859_length_3069_cov_3.207034"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PK768_08735"
     CDS             <1..359
                     /locus_tag="PK768_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQA61096.1"
                     /translation="LSPFKDPMTQVYPVKILVPNEKGLLKSGMFARVKLMVALHTDAV
                     TVPEDAVVSYDGTKIVYTLEGDVAKAITVETGSSSMGKTVIVKGLEAGKEIIVEGQDL
                     LTDGAKVKVEGRGDGK"
     gene            401..3069
                     /locus_tag="PK768_08740"
                     /pseudo
     CDS             401..3069
                     /locus_tag="PK768_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          918 a          646 c          742 g          763 t
ORIGIN      
        1 gcctaagtcc cttcaaggac cccatgacgc aggtttatcc agtaaaaatc ctggtgccca
       61 atgaaaaagg ccttttaaag tccggcatgt ttgcaagagt aaagcttatg gtggcactgc
      121 acaccgatgc tgttacggtg ccggaagatg ctgtggtttc ttatgacggt accaagattg
      181 tatatacttt agaaggcgat gtggcaaagg ctatcacagt tgaaacgggg tcaagttcca
      241 tgggcaagac ggtaatagta aagggccttg aagcaggcaa agaaataatc gtcgaaggtc
      301 aagacctatt aactgacggc gcaaaagtca aagtagaggg ccggggtgac ggaaaatgaa
      361 cttagcccaa ttttccataa aaaggcccgt aactaccctt atgatcatag ccgtagtcct
      421 ggtgctaggt tttatctctt ttacaaaact ggggatagac ctgttccccg atttttcatt
      481 tcctgtaagt gtcgttatta cccaatacca aggggcagct tctcacgaag ttgaaaacct
      541 gatcacaaag cctttagaac agatattgtc aacgatgaac aacgtgaaaa atattcagtc
      601 tctgagcagt gagggcagtt ccataattat tattgaattt aactggggca ccaatatgga
      661 tgtggcaagc caggacattc gggaaaagat tgatttaata gagacctttt tgccttcaga
      721 tgcagaaact cccatggttg tcaaatttga cccgtccatg atgccggtaa tgcagattgc
      781 cctctacggc agcgatgata ttgtccagct caaaaagatt gctgaggaca tcatagaaag
      841 ccgtcttttg cgcatagagg gtgttgcctc ggtggggatt tcaggcggtt tagaaaggca
      901 gatagccata agcgtagacc ctgacaagct tgcctttcac ggcctcagca tgtatcagtt
      961 ggcaagtaaa ttgcaaatgg aaaatatcaa tcttcccggt ggcacagtat accaggggaa
     1021 aaagcaatac agcataagga cgcaagcgga atttgaagac atatcggaaa tcgagaatat
     1081 gcccatcccg ctgccccagg gcggaactat ccccctaaag agcattgcga aagtggaaga
     1141 tacccacaag gatgtatcca caattgcccg ctacaacggc aaacccagca tagcactgac
     1201 catacaaaaa taaagcggct ataacaccgt gcaggttgca aaaaaggtca aagccgagat
     1261 ggaaaaattg aaaagggaaa ttcccgttga aatagattat gacacgattc ttgaccagtc
     1321 ggagttcatc gagttctcta tcaacaacgt gaaagacaat gcgataactg gcggcattat
     1381 tgccgtactg gtcatatata ttttcctcca aaacttgagg agcactttga taatcggcct
     1441 atcgatacct atatcaatca tatcgacttt tgtattgatt tactttaaca aactcacact
     1501 caacatgttg agccttggcg gcttagcact gggtataggc atgctggtgg acaactccat
     1561 agttgttttg gaaaatattt attcgcacag gacgaggggc gaggagccgg taaccgcagc
     1621 cctatcgggc acaaatgaag tagctatggc cataaccgca tctactctta caaccatagc
     1681 ggtatttttg cctatcgtat ttgtggaagg tatgacggca caccttttta aagaactggc
     1741 cctaacggtt actttttccc tactttcttc cctttttgta gcattgacca ttgtaccctt
     1801 actgtcttca agacttatgg cacagattaa agtagaggaa atattatatg cagaagacaa
     1861 cagaaagggt ttagcttctc tcctgcaaaa atttaaggtc ttttacggaa aagttgaaga
     1921 aaagtattct gatctgctga aatggtcgct ctctcataga aaagcgataa taataccggc
     1981 agtgacactc tttataataa gcatggcttt gattccactt ttgggagcag agtttttgcc
     2041 tcgctccgat gcgggttcaa ttagtatttc agtgaaattg ccttacggga caaatcttga
     2101 cgagaccaac cgctttgtat ccgagttggt ggataggata gaagagatac cagaggtaga
     2161 aggtataatg gaaagcatcg gcagcggagg ccaaatgatg ggagacatga cttcagacag
     2221 cagtgtggcc agcgtaaccc tgcagcttgt gcccctggca gagcgtcggc gctctacaga
     2281 tgaagttgct gaagaggtga gaaatctgac agaggatatg gccggagctg aaatagaagt
     2341 cagggcagta agcagcatgg actttgtaag cggtggtgca ctggaatcaa tctctattga
     2401 gattagcggt gatgatcttg acgagcttaa agaaatatcc agtcaggtta ccgaaattgt
     2461 caaagctact cccggcacca gggaagtcaa gagcagcctc gaggaaggca aacccgagct
     2521 cgtcataaag atcgacaggg aaaaggccac tatgtatggt ctttccaccg cccagatagc
     2581 ccaaacggta aattccgcaa tatcagggag tgttgctacc aaatataaag ttggcggaga
     2641 cgagattgat gtagttatca agtcagatga tgaattggtc gatgatatat ccaaaatcca
     2701 agacctgctg attccaagtc ctacaggagc ctttttaact ttaagggacc tggccaaggt
     2761 tgaaagaagc actggtccag ttaccatagt tcggctaaac caggcacgac aggtgtcggt
     2821 aactggagcc gtagttggaa gagacatagg aactgtgaac cgggaaatac agcaaaagct
     2881 tagcgaactc cccttgcctg aaggctacag cataaaaatg ggcggagagc aggaacagat
     2941 gatggaatcc ttcagagaat tgatattggt gttcttactg gctgtagtcc tagtgtatat
     3001 ggtcatggcc gcccagtttg aatccctaaa acatcccttt gtaatcatgt ttaccgttcc
     3061 cttggcttt
//
LOCUS       DAONUO010000258         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21884_length_3066_cov_4.368316, whole genome shotgun sequence.
ACCESSION   DAONUO010000258 DAONUO010000000
VERSION     DAONUO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21884_length_3066_cov_4.368316"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /gene="aroD"
                     /locus_tag="PK768_08745"
     CDS             <1..651
                     /gene="aroD"
                     /locus_tag="PK768_08745"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HQA61097.1"
                     /translation="ACDPEKVLLALRLIRSIIGEIPIIFTFRSSLEGGNMEVDDDIRY
                     EIIEKAINTKKVDVIDIELISGKTNIRRIKAATVKQNIPLILSYHNFKETPSVEFMLD
                     KMKEQVLNGADIAKIAVMPKDEEDVLNLLSATLKARKEMPGTPLITMSMDELGVITRI
                     AGGVFGSDLTFGACGKNSAPGQISVVELREAMKTVYGGSIYNSASFTSTDYAVPDY"
     gene            683..1588
                     /gene="aroE"
                     /locus_tag="PK768_08750"
     CDS             683..1588
                     /gene="aroE"
                     /locus_tag="PK768_08750"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQA61098.1"
                     /translation="MVNINSKTKLVGLLGYPLDHTLSPAIHNAAFEEKRINNVYLPIE
                     VKPDNFGHVIKTISCMNFIGFNITIPFKVEVMKYLDAIDETAKCIGAVNTVVVRDGKL
                     IGYNTDGTGFLKSFEEASNSTIAEKKVLVLGSGGASKAICMTLALNKVGKLYICNRTH
                     EKALTLASSINKKVSAISSAIPMEYNAINETIKKVDLLINTTSLGMFPRTDEMPIDGS
                     LLNKGLIVCDIVYNPRKTKLILEAERLGCKTVPGLGMLVYQGAEAFEIWTGTKAPVKL
                     MYSVVNEALERNGFKRIEKPDKPEF"
     gene            1668..2486
                     /locus_tag="PK768_08755"
     CDS             1668..2486
                     /locus_tag="PK768_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQA61099.1"
                     /translation="MDRKYSLAHLTVLKWTIPEMIYNAKLIGYDYVGIRNIPMGVTGE
                     AEDGLSKRNGRYKLTKKALDDTGMKIHDVELTMIRDDKDVSSYEPALEIAAELGATDV
                     ISSIWTPNKAYYIEQFAKLCDLAKQYDLFVNLEFVTWAEVKNLDEAKEVISTVGKENA
                     GYLIDTLHFHRSRVQLSELKDIPKKQLRMAHICDGPGEIPSDKESLIFTGRDARLYLG
                     EGAINVDEIIGQLPNDIVLSIELPNIQRVKEYGYTEHARRCLETAKEYFARRCQ"
     gene            2676..>3066
                     /locus_tag="PK768_08760"
     CDS             2676..>3066
                     /locus_tag="PK768_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61100.1"
                     /translation="MITRVVKNSVRGEITVPSSKSHTIRAVTIATLAKGKSIINNPLP
                     SEDCKAALKAAEKFGAKCEVYEDKWIVEGLGSEIQVPDDVIDVKNSGTTLYFMASVAA
                     LLTDYTVFTGDKSIRSRPITPLLNALEQ"
BASE COUNT         1096 a          443 c          717 g          810 t
ORIGIN      
        1 gcatgtgacc cggaaaaagt tctattggca ctaagactaa ttagaagcat catcggtgaa
       61 atacctatta tatttacatt cagaagcagc ttggaaggag gcaatatgga ggtagacgat
      121 gatatacggt atgaaataat tgaaaaagcg attaatacaa aaaaagttga cgttatagac
      181 attgaactaa tcagcggtaa aaccaatatt aggagaatta aggcagcgac agtcaaacag
      241 aatataccgc ttattctatc ctatcacaac tttaaagaaa caccatctgt agaatttatg
      301 ctggacaaga tgaaggagca ggtattaaac ggtgcagata tcgctaagat agctgttatg
      361 ccaaaagacg aggaagatgt cctaaacctg ttatcagcta cattaaaagc ccgtaaggaa
      421 atgccgggta ctcctcttat aacgatgtcg atggatgaac ttggagtgat aacaagaata
      481 gccggtggag tattcggttc tgatctgacc tttggtgcgt gtggaaaaaa ttcagcaccg
      541 ggacagatat cggttgtaga attaagagag gcgatgaaaa cagtatatgg aggctcgatt
      601 tataacagtg catcttttac cagcactgat tacgcagtac cagattatta attaaaacga
      661 acaaagcatt tggggaggtt ctatggttaa catcaattca aaaacgaaac tcgttggatt
      721 attgggatat cctttggacc atacattgtc tccagccata cataatgcag cttttgaaga
      781 aaaaagaatt aacaatgtgt atttgccgat agaagttaag cctgataatt ttggacatgt
      841 gataaaaacg atatcatgca tgaattttat tggttttaat ataaccattc catttaaagt
      901 cgaggtgatg aaataccttg atgctattga tgaaaccgct aagtgcatag gggcagtaaa
      961 tacagttgtg gtaagggatg gaaagcttat tggatataat actgatggaa caggatttct
     1021 gaaatctttc gaggaagcat caaattctac aatagcagaa aagaaagtgc ttgtgttggg
     1081 tagtggcgga gcttcaaaag caatatgtat gacactggca ttaaataaag taggaaaact
     1141 gtatatttgt aacaggacac atgaaaaagc tttgaccttg gcgagcagta taaataaaaa
     1201 agtgtcagcg ataagctctg cgattcccat ggaatataat gcaattaatg agacaataaa
     1261 aaaagttgat ttgttgatta atacaaccag tttagggatg tttccaagaa ctgatgaaat
     1321 gcctattgac ggcagcctgc tgaacaaggg gttaattgtt tgtgatattg tttataatcc
     1381 aagaaaaaca aaactgatac tggaagctga gagactgggc tgtaagactg tgccaggatt
     1441 aggaatgctt gtataccaag gggctgaagc atttgaaata tggactggaa caaaggcacc
     1501 ggttaaattg atgtatagtg tagtaaatga agctttggaa cgaaatggtt ttaaaagaat
     1561 tgaaaaacct gacaagccgg agttttgatt ttgaaaggtt tgtgttactg cttataaaca
     1621 agggttaaaa atactgaagg cagttatttg aagggagaat aacgattatg gatagaaaat
     1681 attcactcgc acatcttaca gtattaaaat ggacaatacc tgaaatgatc tataatgcta
     1741 agctgattgg ctatgattat gtaggaattc gaaatatacc gatgggagta acgggagagg
     1801 cggaggatgg cctgtctaaa agaaacggac gatataagct tacgaaaaaa gcccttgacg
     1861 ataccggtat gaagattcat gatgtagagt tgaccatgat aagagatgat aaagacgtga
     1921 gttcatatga acctgcatta gaaattgctg ccgagctggg agcaacggat gttattagca
     1981 gcatatggac accaaataaa gcttattata ttgaacagtt tgctaaacta tgcgatttgg
     2041 caaaacaata tgatttattt gtaaatctgg agtttgttac gtgggctgaa gttaaaaact
     2101 tagatgaagc aaaagaggtg attagtaccg ttgggaaaga gaatgccggg tacttgattg
     2161 atacattgca tttccatcgt tcaagggttc aattatctga attgaaagat attcctaaaa
     2221 agcagctccg tatggcacat atatgtgatg gccccggtga aataccgtca gacaaagaaa
     2281 gccttatatt taccggtaga gatgcaaggt tgtatttggg agaaggggca ataaatgttg
     2341 acgaaataat agggcaactg ccaaatgaca ttgtattatc aatagaatta cctaatatcc
     2401 aaagagtaaa agaatatggt tataccgaac atgcgcggcg atgtcttgag acagcaaagg
     2461 agtatttcgc aagaaggtgt caataacagt tgttgtatac gacagaagat ataagcctga
     2521 tattgtcgga caactgataa tcggtcataa aaactgctaa gtaaaagtca atattactaa
     2581 gttagcaata gtttgtcgga aaggaggaag ttgcgagaat taagtaaaga tgagttgaaa
     2641 tgaagaatta caaaaatgat taaggggggg ggaacatgat aacaagagtt gtaaagaaca
     2701 gtgtaagagg agaaattact gttccaagct caaaaagtca tacaataaga gcggtaacaa
     2761 ttgccacatt ggcaaagggg aaatcgataa tcaataatcc gctgccaagc gaagactgca
     2821 aagcagcgtt aaaagctgca gaaaaattcg gtgccaaatg tgaagtgtat gaagacaagt
     2881 ggattgttga agggttgggt tcggaaatac aggtgccgga tgatgtcata gatgttaaaa
     2941 attcgggcac tacgctgtat tttatggcat ctgttgctgc tttactaaca gattataccg
     3001 tttttacggg ggataaatcc ataagaagca ggcctataac accgttgtta aatgcacttg
     3061 aacaac
//
LOCUS       DAONUO010000259         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21902_length_3065_cov_3.195349, whole genome shotgun sequence.
ACCESSION   DAONUO010000259 DAONUO010000000
VERSION     DAONUO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21902_length_3065_cov_3.195349"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PK768_08765"
     CDS             complement(<1..564)
                     /locus_tag="PK768_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HQA61101.1"
                     /translation="MVFKRCLTVILIAALLIFVQGCFSKKDDVESEESSDMEHQDEIT
                     EFEETIPDTPKAKNPLTGMDMDIKFEKQRPFAVMIENEYNARPQSGLNKAGVVYEILT
                     EGGITRFLAIFLGKTLNEIGPVRSARPYYLDYAMEYDSIYVHYGASPQGYIDLKQLKI
                     DAIDGIYDDVTFWRDKSRKAPHNAYTST"
     gene            complement(599..970)
                     /locus_tag="PK768_08770"
     CDS             complement(599..970)
                     /locus_tag="PK768_08770"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HQA61102.1"
                     /translation="MKKARTLAESFFYAISGILYAFKTQRNIKIHFMVAILIIIISFL
                     LKLNTIELLIVFLTIAMVLTAEMINTAIEAVVDMFTQEYHPLARVAKNVAAGAVLISA
                     LNAVIVGYLIFYKKIAVLLGI"
     gene            complement(971..1450)
                     /gene="ybeY"
                     /locus_tag="PK768_08775"
     CDS             complement(971..1450)
                     /gene="ybeY"
                     /locus_tag="PK768_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQA61103.1"
                     /translation="MANIMINNLQDKIDVTEELKALVIKAVNSTLSVEKVPEKVEVSI
                     ALVDDKYIQELNRQYRNVDAPTDVLSFAMRERAAGEDNIDFCEEELLGDVVISLERAT
                     MQAAEYGHSFEREVGFLVVHGVLHLLGYDHESDEEKKLMRQKEEEILKSIDLSRGIS"
     gene            complement(1410..>3065)
                     /locus_tag="PK768_08780"
     CDS             complement(1410..>3065)
                     /locus_tag="PK768_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQA61104.1"
                     /translation="KAIIETVMEEGVKEDSIDRTKTYVIEEFKSLNIPQELKGIGEEI
                     ALLTIRPNMVYDREATERQQREAMEAIAPVRVFPNQVLIEKGTVITSEHIEMLKEMGL
                     LARDRKADISLLIGVMCVSAFLVASLTFAVYYFHRDVYNNNLYLTLLILIILSTLIIS
                     LGVKNISNYLIPLAAGSMLISILINPQVAVFASFTMSVVVGIMLGNDFSHALVALSGA
                     LIGIFCTAKVSQRSDLTKAGGIIGGAKFILITGTMLLNNSTALEILKESPWGLVSGIL
                     SAILAIGTLPFLENAFGITSAIKLLELSNPNQPLLRKLMLDAPGTYHHCIIVGNLAEA
                     GAEAVGADSLLARVGANYHDIGKLKRPYFFIENQLTSDNPHDKLSPTLSALIITSHVK
                     DGVEIAKEYRLPDCITDFIVQHHGTSLLSFFYQKALESADEKKPDEASFRYEGPKPKT
                     REAAIVMLADCVEAAVRSLPKPTHGKIEGLIRQIIKDKLSDGQLDECDLTLKDLDKIA
                     VAFSKIMTGIFHTRIEYPDKLKELQRKDLNNGQYNDKQFARQN"
BASE COUNT          822 a          675 c          581 g          987 t
ORIGIN      
        1 ggtgcttgtg tatgcattat ggggtgcctt tctggacttg tcgcgccaga acgtcacatc
       61 atcatatatt ccgtctatag catcgatttt tagctgtttc aaatctatgt atccctgagg
      121 gcttgctcca tagtggacat atatactgtc atactccatg gcataatcaa gatagtacgg
      181 tctggcgctt cgtacgggcc cgatttcatt caatgtcttt cctagaaata ttgccaaaaa
      241 gcgggtaatg ccgccctcag ttaaaatctc gtaaactaca ccggctttgt tgaggcccga
      301 ttgaggtctg gcattgtact cgttttctat catgactgca aatggcctct gcttttcaaa
      361 ttttatatcc atatccatac ccgtaagggg attttttgct ttcggagtat cgggaatggt
      421 ttcctcaaac tccgtgattt catcctgatg ctccatatct gaagattctt cagattctac
      481 atcatccttt ttgctaaagc atccttgaac aaatataagt agtgctgcaa ttaatattac
      541 agttaggcat cgcttaaaga ccatgtcttt accctcctaa tgtttcatga gaggcttttt
      601 aaatgccgag tagcacagct atttttttat aaaatattag ataaccgacg attacagcat
      661 ttagagctga tataagcact gcacccgctg ctacgttttt agcaactcgt gctaagggat
      721 gatattcctg ggtaaacata tctactacag cttctatagc agtatttatc atctcagcag
      781 tcaataccat ggcaattgtt aggaatacga tcaaaagttc aattgtgttt agctttagca
      841 aaaagctaat aataattatt aaaatggcaa ccataaaatg aatctttatg ttgcgctgag
      901 ttttaaaggc ataaagtatg cctgaaatgg cataaaaaaa actttcagcc aaagttctag
      961 ctttcttcat ttagctaatc cctctagaca aatctattga tttcaggatt tcctcttctt
     1021 tttgcctcat aagcttcttt tcttcatccg attcatggtc gtatcccaat aaatgtaata
     1081 cgccgtggac gactagaaaa cccacttccc gctcgaatga gtggccgtat tctgcagctt
     1141 gcatagttgc cctttcaagg gatattacca catcgccgag gagctcttcc tcacaaaaat
     1201 caatattgtc ttctccagcc gccctttccc tcattgcaaa agataacaca tcagttggag
     1261 catctacgtt gcgatactga cggttgagct cttgaatata tttgtcatca accaatgcta
     1321 tgctgacttc tactttctct ggtacttttt ctacagataa ggtgctattt acggctttga
     1381 tgaccaatgc tttcaattct tccgttacat caattttgtc ttgcaaattg tttatcatta
     1441 tattggccat tgtttaaatc cttcctctgc agttctttta atttatcggg atattctatc
     1501 ctggtatgaa aaattccggt cataattttt gaaaacgcta cggctatttt atctaaatct
     1561 tttagcgtca aatcacattc atctaactga ccgtccgaca atttgtcctt tattatttgc
     1621 ctgattaatc cttcgatttt cccatgagtg ggcttgggca gggacctcac tgcagcttca
     1681 acacagtctg ccaacataac tatagctgct tcccgggtct tgggtttagg cccttcatag
     1741 cgaaaactgg cctcatcagg ctttttttcg tcagcactct ctaaggcttt ttgatagaaa
     1801 aaggaaagga gggaagtccc atgatgctga acaataaagt ctgtaataca gtcaggtaag
     1861 cggtattcct ttgctatttc caccccatcc ttaacatggg aagtgattat taatgcgcta
     1921 agggttgggc tcaacttatc gtggggattg tcggaagtca gttggttttc tataaagaaa
     1981 tatggcctct ttagtttgcc gatatcatga tagtttgcgc ctacgcgcgc taatagggaa
     2041 tcggctccaa ccgcttcggc tccggcttca gctaaattcc ctacgataat gcagtggtga
     2101 taggtaccag gagcatctaa catcaatttc ctcagcagag gttgatttgg gtttgacagt
     2161 tctaacaatt ttatggcact agttatacca aaagcatttt ctaaaaatgg cagggtgcca
     2221 atcgccaata tggcggacag aattccgctt actaggcccc aagggctttc cttcagtatt
     2281 tctaaagctg tgctgttgtt taaaagcata gtgccggtta tgagaataaa tttagcaccg
     2341 ccaattatcc ctcctgcttt tgtcaggtcg gaacgctgag aaaccttggc agtgcaaaaa
     2401 ataccgatta aggctcctga cagagccaca agggcatgag aaaaatcatt gcccaacata
     2461 attcccacta caacgctcat tgtaaaactt gcaaacacag ccacctgagg attgatcagg
     2521 atggatatga gcatacttcc ggcggctaat ggaatcaaat agtttgatat gtttttaact
     2581 cccagggaaa ttattagggt tgataatatg ataagtatca ataatgtcag gtatagatta
     2641 ttgttgtaaa catctctgtg aaaatagtat acggcaaagg ttagggaagc caccagaaaa
     2701 gccgatacgc acattacgcc aatcagcaaa ctgatgtcag cttttctgtc ccttgccagc
     2761 agtcccattt cttttagcat ttctatgtgt tcggaagtta tgaccgtacc cttttctatc
     2821 aatacttggt ttgggaaaac tctcacaggt gcaatggctt ccatggcctc ccgctgttgc
     2881 ctttcggtag cctcacggtc atataccata ttgggtctta ttgtcaatag tgcgatttct
     2941 tcaccaatac cctttaattc ctggggtata tttaagcttt taaattcttc tataacatac
     3001 gtcttagtcc ggtcaataga gtcttctttg acaccttcct ccataaccgt ctctatgatg
     3061 gcttt
//
LOCUS       DAONUO010000260         3055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_21982_length_3055_cov_3.091667, whole genome shotgun sequence.
ACCESSION   DAONUO010000260 DAONUO010000000
VERSION     DAONUO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21982_length_3055_cov_3.091667"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PK768_08785"
     CDS             <1..576
                     /locus_tag="PK768_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HQA61105.1"
                     /translation="ANMAGFDLIELHGAHGYLINQFLSPLTNKRVDEYGGSLENRVRF
                     LKEIITAVRQVWPMEKALMLRVSAEEYAQGGNHAEDVAQIINLLKDEGIDMINVSSGA
                     VVPVKMKVYPGYQVTYAETIKRLTNLPVIAGGLITEPAMAEEILQNERADMVYIGREL
                     LRNPYWPLQAAKKLNVDIIWPSQYERAKIKI"
     gene            complement(620..1918)
                     /locus_tag="PK768_08790"
     CDS             complement(620..1918)
                     /locus_tag="PK768_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQA61106.1"
                     /translation="MSKPKVAIVKCDTPEGNAEFMAGKFVRYDSDIAKIKEAVAKAVE
                     LAIGSPDAIIKPGQKVLIKPNLAFQAPPESFAVVDPRVVEAVVAYFKENSKAGEIWVG
                     DNPSLGLHVGRAKPAFEASGMEEAARRGGADKVIYFDETATIEVDIPEAKVFKKAAIF
                     KPVLDADVIINLPKMKVHLAGTVTLALKNWNGLIPNVHPSDQQQGAHRIDLGQKFADL
                     YRAVKADLTIVDAIIGMEGQGPHAGTPIEMNLIIAGNDMVATDAVSSYVMGFDPIEIP
                     AIRCAVTEGLGVMDLDKIEVLGETPDSVRKIFKRPNDNPVGMYRGFTVFVQQTCPGCY
                     VNVRGALDSFAGTGISMEEFIEEKGEVVVVAGGVPDFDPEFARGKYMFVCGDCWEYFP
                     TADKVRKAAEIAKEVIYYPGCAPVYIFSQLNTDLQKLARG"
     gene            complement(2274..>3055)
                     /locus_tag="PK768_08795"
     CDS             complement(2274..>3055)
                     /locus_tag="PK768_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HQA61107.1"
                     /translation="GFKSINVTLRQTFDLYANIRPVKTLPGINGLFKDVDIVIFRENT
                     EDLYAGIENIVDEDRVEAIKVITRKASRRIVEKAFEYAVLHGRKKVTAIHKANIMKKS
                     DGLFLEEARKVAEKYRDINYEELIVDNACMQLVQKPGKFDVIVTENLYGDIISDLCAG
                     LVGGLGVVPGINEGQELTIFEAVHGTAPDIAGKNIANPLALMLSAVEMLKHFGEHTAA
                     KAIHKAIHDLLQEGKCLTPDLGGSATTKEMTKELCRLVNYC"
BASE COUNT          759 a          756 c          664 g          876 t
ORIGIN      
        1 gccaatatgg ccggctttga tttgatagag ctgcacggtg ctcacggtta tctcatcaat
       61 caattcctat ctcccttaac caataagcga gtggacgaat acggcggcag ccttgaaaat
      121 cgggtgcgct ttttaaaaga gattattaca gccgtaagac aggtttggcc catggaaaaa
      181 gccctaatgc ttcgggtttc tgccgaagag tacgcacagg ggggcaatca tgcggaggat
      241 gtggcccaaa tcattaatct tcttaaagat gaaggtatag acatgataaa tgtcagctcc
      301 ggggcagttg tgcctgtcaa aatgaaggta tatcccggtt atcaggtcac atatgcagag
      361 accataaaga gattgaccaa tcttcccgtc attgcagggg gactaattac agagccggcc
      421 atggccgagg aaatcctgca aaacgaacgg gcggacatgg tatacatcgg aagggaactg
      481 ttaagaaacc cttattggcc tttacaggcg gccaagaaac taaatgttga tataatctgg
      541 ccatcccagt acgaaagggc caaaataaaa atttaagacg gtgatgctag catcaccgtc
      601 tcattatcac gtaaatatat tagcctctgg ccagtttctg taaatcggtg ttgagttgtg
      661 agaagatgta taccggagca cagccgggat agtagatgac ttcctttgca atttcagcag
      721 ccttgcggac tttatcggca gtggggaagt actcccagca atctccgcaa acaaacatgt
      781 acttacctct ggcaaattcc ggatcaaaat ccggcacgcc tcctgccaca accactactt
      841 cgcctttttc ttctatgaac tcttccatgc taatgccggt acctgcaaag gagtctaggg
      901 cacctctcac gttgacatag cagccggggc aggtctgctg cacgaagacc gtaaagcctc
      961 tgtacatacc taccgggtta tcattgggcc gcttgaatat cttcctgacc gaatcggggg
     1021 tctcgcccag cacttctatc ttatccagat ccatcactcc gagcccttcc gtgactgcac
     1081 agcgtattgc cggtatttca atggggtcaa agcccattac ataggatgat actgcatcgg
     1141 tggccaccat atcgttaccg gcaataatta ggttcatttc aatgggggtt ccggcgtgag
     1201 gtccttgacc ttccatgccg atgatggcat ctactatggt cagatctgcc tttacagctc
     1261 gatataaatc agcaaatttc tggcctaaat ctatgcgatg ggctccctgc tgctgatcgc
     1321 tcgggtggac attcggtatc aagccgttcc agtttttgag ggccagtgtc accgtgcctg
     1381 ccaagtgaac tttcatcttg ggcaggttga tgattacgtc agcgtccagc acgggtttga
     1441 atatggcagc tttcttgaag accttggctt cgggaatatc aacttctatt gttgcagttt
     1501 cgtcaaagta tattactttg tcggcgccgc ctctacgtgc cgcttcttcc atgccggatg
     1561 cttcaaaagc cggcttggca cgacctacat gaagacccaa cgaaggattg tctcctaccc
     1621 aaatttctcc tgccttcgag ttctctttaa agtacgcaac taccgcctct actacccttg
     1681 ggtctaccac cgcaaagctt tccggtggtg cctggaatgc caggttgggc ttgatgagga
     1741 ctttctgacc gggtttaatg atagcatctg gtgatcctat agcgagttct actgcttttg
     1801 ccactgcttc ttttatcttg gctatatccg agtcataccg cacaaactta cctgccataa
     1861 attcagcatt accctccggg gtatcgcact tgacaatggc cactttgggt ttactcacta
     1921 tgtatcactc ctttttaatt ttttattaag acaaggcata gacaacaaaa gcaatgcctg
     1981 acctaatctt ttccctcctc tcgtatctga cattgcttgt atcattatat tagtgctatg
     2041 gccgtattta tgtctgtttt ttgataaatt gcgaaatgta agtgaaagtc agtttttaga
     2101 cctttctaaa gaattttgtt gtttaaattt ttatccattt attattatat aatcattatt
     2161 cgtatttttc aatgtccaac ggacatctct atagcaaaaa aacctctaga caataaaaaa
     2221 atgccagcac aagctggcaa tatctatgga aaggaaggag taaaaagcta agttcaacag
     2281 tagtttacca agcggcaaag ctccttggtc atctccttcg tagtagcgct gccgcccagg
     2341 tccggagtga ggcatttccc ctcttgaagt aggtcatgaa tggccttgtg tattgctttg
     2401 gctgcagtat gctcgccaaa gtgttttagc atctccaccg ctgacagcat cagggccagg
     2461 ggatttgcta tgttctttcc cgcaatatcg ggggcagtcc cgtgaaccgc ctcaaaaatc
     2521 gttaattctt gcccctcatt tatacccgga actacaccca ggccacctac gaggcccgca
     2581 cacaggtccg aaatgatgtc accgtatagg ttttccgtga cgatcacatc aaatttccct
     2641 ggcttttgca ctaactgcat gcaggcattg tcaacgatca actcttcgta gtttatatcc
     2701 ctgtattttt ccgcaacttt gcgggcttct tctaaaaaca ggccatcgga ttttttcatg
     2761 atatttgcct tgtgaattgc ggtgaccttt ttccgtccgt gcaaaacagc gtattcaaag
     2821 gccttctcca caattcttct gctggccttg cgggttatta cctttatcgc ctccacccta
     2881 tcctcatcca cgatattttc gatgcctgca taaaggtcct cggtgttttc cctaaaaatt
     2941 acgatgtcca catccttgaa gaggccattg attccgggca gagtttttac aggcctgatg
     3001 tttgcataca ggtcaaaggt ctgccttagg gtcacattga tgcttttaaa acctt
//
LOCUS       DAONUO010000261         3034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_22174_length_3034_cov_3.987244, whole genome shotgun sequence.
ACCESSION   DAONUO010000261 DAONUO010000000
VERSION     DAONUO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22174_length_3034_cov_3.987244"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PK768_08800"
     CDS             <1..874
                     /locus_tag="PK768_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA61108.1"
                     /translation="YKLEEYLNHEDAPLINRAVESYTPGSIFKIVILAAALEEKLADL
                     NEVFYCKGFEQVGGNIFKCSSYDEGGHGELTLKSALAYSCNSVFIQLGMRLGEDKILK
                     YARLLGLGEKTRIGLPEEKPGNIPKDGEVYYQDIGNISIGQGAIGITPIQAAQLLLTI
                     VNDGVFKRPVLVKHIRDSAGNIHKNLGFQSPKDNVLSPETAQRVREALEDATKYGTGF
                     RANPYKNHSIGGKTGTAETANGSPHAWFVGYYPAEKPELIICVFAEHGGSGSLKAATV
                     FKEIVCQITSLTEN"
     gene            940..1659
                     /gene="sigK"
                     /locus_tag="PK768_08805"
     CDS             940..1659
                     /gene="sigK"
                     /locus_tag="PK768_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HQA61109.1"
                     /translation="MENALSVLVTLGVLFFKEILTWLAFITSSTTFPKPLSPEEEEKY
                     LNLFYEGSEEARNILIEHNLRLVAHIVKKFASTGEDMDDLISIGTIGLIKAITTFNQD
                     KGSRLATYAARCIENEILMNLRSTKKVKAEVSLQDPIGIDKEGNEISLIDVLGTESDA
                     VANEISKKFQQEKLYEKIRTALKCRERKVIELRYGLINGATKTQREIAKLLGISRSYV
                     SRIEKRAIKKLCKELHSEGCK"
     gene            1715..2227
                     /locus_tag="PK768_08810"
     CDS             1715..2227
                     /locus_tag="PK768_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HQA61110.1"
                     /translation="MLKLFCPDLYLESIYKLDLDSIKEKNIKGILVDLDNTLLPWDSK
                     HVEEKLIGWTQYCLKEGFSLCIISNNKYKRIKRCSELLGIPAVFGSYKPFKSAFKKGM
                     DIMGTQPEQTAVIGDQIFTDVFGAKRMGLYAILVKPISSRELYWTRIMRLMERRIIKI
                     LENKKLITLD"
     gene            2253..2465
                     /locus_tag="PK768_08815"
     CDS             2253..2465
                     /locus_tag="PK768_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61111.1"
                     /translation="MELLTINREMPRFLAINLHKPCIVINELKKIVKDLKQKNPHLNN
                     YCLMEVGFSPDKEISHMRLYFMKKSQ"
     gene            2560..>3034
                     /locus_tag="PK768_08820"
     CDS             2560..>3034
                     /locus_tag="PK768_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HQA61112.1"
                     /translation="MKSLEDYLIKENYLTKNDLEKIKEIQVKTDKRLDEVLIDEGFIT
                     ETKMAEILAITAGVPSVNLNKLYISPDVIRLVPEYLARKHLALPIKKDGNTLFLALSD
                     PFNIFAIDDIKLATNYNIQVVIASKKEIQKAIDIYYKLHADSTVYSIREDNHEVVD"
BASE COUNT         1066 a          504 c          637 g          827 t
ORIGIN      
        1 atataaactg gaagagtatt taaatcatga agatgccccg cttattaatc gggccgttga
       61 aagttatact cccggttcga tatttaaaat agtaatatta gctgcagccc ttgaagaaaa
      121 actggcagat ttaaatgagg ttttttactg taaaggtttt gagcaggttg gaggcaatat
      181 ttttaaatgc tccagttacg atgaaggggg ccatggagaa ctaaccttaa aaagcgccct
      241 agcctattcc tgtaattcgg tttttattca attaggtatg cgtttaggtg aagataaaat
      301 tcttaaatat gcaaggcttt taggtttagg cgaaaagaca cgcataggct tgcctgaaga
      361 aaaacccggc aatatcccta aagatggaga agtatattac caagacatag gcaatatttc
      421 aataggtcaa ggggcgattg gtataacacc tattcaggca gcgcaacttt tattgacgat
      481 agttaatgat ggtgttttta aaagacctgt gcttgtcaaa cacataaggg atagtgccgg
      541 aaacatccac aagaatttag gttttcagtc cccaaaagat aatgtactgt cacctgaaac
      601 cgctcaaagg gtaagggaag cattagaaga tgcgacgaag tatggtaccg gctttagagc
      661 caacccttac aagaatcaca gtattggcgg gaaaacaggc accgccgaaa ctgcaaacgg
      721 cagtccccat gcctggtttg tcggttatta tccagcggag aaaccggaac ttataatatg
      781 tgtatttgcg gagcatggcg gttccggctc tcttaaagca gctacagttt tcaaggaaat
      841 agtgtgccaa atcacttctt tgacagaaaa ctaaataaac atgcacattt tctgactcaa
      901 agtcatatat tataggtacc gcacagggga ggtgctaaaa tggaaaatgc cctgtcagtc
      961 ctggtgactt tgggtgtttt attttttaaa gagatattga catggttggc atttataact
     1021 agctctacaa catttcccaa gcctctcagc ccggaagaag aggagaagta tttaaaccta
     1081 ttctatgaag ggagcgaaga agccagaaac atcctgattg aacacaattt gagattggtt
     1141 gcccatatag tgaaaaagtt cgcaagcacc ggggaagaca tggatgattt aatatccata
     1201 ggaacaatcg gccttataaa ggccattact acttttaatc aagataaggg cagccgcctt
     1261 gccacttatg cagctcgctg catcgagaat gaaatactta tgaatttaag atcgacaaag
     1321 aaggtcaaag cagaggtttc actacaagac cctatcggta tagataaaga aggcaatgaa
     1381 atttctctca ttgacgtatt ggggactgaa tctgatgcgg ttgccaacga aatatcaaaa
     1441 aaatttcaac aggaaaaact ctatgaaaaa atcaggacag cattaaaatg ccgggagcga
     1501 aaagtcatag aactgcgata tggccttata aacggggcca ctaaaaccca gagagaaata
     1561 gccaagctgc ttggaatttc aaggtcatac gtatcaagaa tagaaaagag agcaataaaa
     1621 aaactgtgca aggagctgca ttctgagggt tgcaaataag ctgttaatta tttaatatat
     1681 atttacgcaa aaactttgta agaagaggcg aatcatgctg aaattatttt gcccggattt
     1741 atatttagaa agtatttaca aattggattt agattccatc aaggaaaaaa atatcaaagg
     1801 gatattagtt gatcttgaca atactctgct tccctgggat tcaaagcatg tcgaagaaaa
     1861 attgataggt tggacacaat actgcttaaa agaaggcttt tccctttgca tcatatctaa
     1921 caacaaatac aagcgcataa aacgttgttc ggaactatta ggaattcccg ccgtattcgg
     1981 atcctataaa ccctttaaaa gtgcttttaa aaaaggcatg gatataatgg gtacacagcc
     2041 tgaacaaact gcggtcatag gagatcaaat atttaccgat gtttttggtg caaaacgaat
     2101 gggcctttat gctattttag taaaaccaat cagcagcaga gaactttatt ggacgagaat
     2161 aatgagactg atggaaagac ggattataaa aatattagag aacaaaaagc tgataactct
     2221 tgattgagta gtggaggtta ggggagatgt cggtggaatt gttgacaata aatagggaaa
     2281 tgcccaggtt tttggcaatc aacctgcata aaccctgtat tgtaatcaat gaattaaaaa
     2341 agatagtaaa ggatttaaag cagaaaaacc cccatttaaa caattactgt ctaatggaag
     2401 ttggtttttc gcccgataaa gagatatcac acatgagatt gtattttatg aaaaaaagcc
     2461 agtaaggatt ttttattttt ccctgtcaaa atatgaagga ttttcccgaa aattgacgaa
     2521 tagtagtata tacatgttcg gggagtgatc ttacgactta tgaaaagcct tgaagactat
     2581 ttgataaagg aaaactacct gacaaaaaat gacttagaaa agattaaaga aattcaagtt
     2641 aaaacagaca aaagattgga cgaagtatta attgacgagg gttttatcac agaaactaaa
     2701 atggctgaaa ttctagctat taccgctggc gtgccaagtg ttaatttaaa taagctttat
     2761 attagtcctg atgtgatacg gctagtgccg gagtatttgg ctagaaagca cttggcatta
     2821 cctattaaaa aagatggaaa caccttgttc ttagcgctaa gtgacccttt caatatcttt
     2881 gcaattgatg atatcaagct agcaactaat tataatatac aggttgttat agctagcaaa
     2941 aaagaaatcc aaaaagccat agatatttat tataaacttc atgctgattc tactgtttat
     3001 agtatcaggg aagataatca tgaagtcgtt gacg
//
LOCUS       DAONUO010000262         3005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_22438_length_3005_cov_3.310508, whole genome shotgun sequence.
ACCESSION   DAONUO010000262 DAONUO010000000
VERSION     DAONUO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22438_length_3005_cov_3.310508"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PK768_08825"
     CDS             complement(<1..471)
                     /locus_tag="PK768_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA61113.1"
                     /translation="MLKVDNLSVTYCQDNTKVPAVKETSLVLEPGQALGIIGESGSGK
                     TTLGLAIMGILNSAAKVEGRITYNGVNLLELSPAQLNEYRWKHIAMVFQNGLETLNPV
                     ITVREQILECIARHLNMKGKEAGRRVSDLLEQVGLEPLVADCYPHQLSGGMRQKV"
     gene            complement(506..1342)
                     /locus_tag="PK768_08830"
     CDS             complement(506..1342)
                     /locus_tag="PK768_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA61114.1"
                     /translation="MLHILKGFSIAGKISVLLILALCFMAVFAPQLVRHPWDTPSGPA
                     LQAPNREHILGTDDLGIDLWAQICYGARNSILVGIGTALLSSLIGVFLGIVSGYFGGI
                     TDLIVTAAIDILMAIPSLPIMIVAGAFFGPSTKNIIIILSLLSWVMPARIIRSKVLAL
                     KEESYIKVAKGYGASFSYITFKHFMPQIFPICATSFIKLISRAIVSEASLSFLGLGDP
                     TSKSWGLILNFALNFRGIYFTDYWKWWVVYPLAFIIILVLSTATLSRELERVLDIKIA
                     GQ"
     gene            complement(1355..2323)
                     /locus_tag="PK768_08835"
     CDS             complement(1355..2323)
                     /locus_tag="PK768_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA61115.1"
                     /translation="MFNKKILKLFLTFFTIITLNFSLPRLMPGDPFYFLSGEQGEITV
                     SYSDEEIQRQKAYYGLDKPVLVQYGRYLVNLARGDLGYSIYFSNDVWTIIKNRIPWSV
                     SLVLLSVIIGGFAGTILGCLSAWHRKGPIDNILYSTMVFVSEIPPFLIGILFLFYLAA
                     QQGLFPLSGGMTPFADYATWMERLLDIVHHGFLPALTLSAVQMGGFFLIARNSMITVM
                     NKDYMTTARAKGIGTARILFRHALSNAALPIVTRMFNSIGQAVGGTILVENVFKYPGL
                     GLLMRQAVLVRDYPLIQGIFLTVTLFVLIMNYAADIIYKKLDPRIV"
     gene            complement(2326..>3005)
                     /locus_tag="PK768_08840"
     CDS             complement(2326..>3005)
                     /locus_tag="PK768_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA61116.1"
                     /translation="RKAIAHAIDIDELIEKVARGAAVPASPGYLPVDHIWYNPDVTKY
                     EFDIEKAKEFLNDRTLDLTLLIADDQMEARIAELLKINLAKAGINLSVQSTDMKTRDS
                     RVKEQNYELALIGHGGWGQDADILRENYGVTVARDNSPASNVIPGYSNLRITELCKKQ
                     LTELDEESRKSMIMELQKLISDEIPLLPLYNTVEYTVFKPEKYDGWVHVFDHHETTHN
                     KLSYLER"
BASE COUNT          777 a          726 c          679 g          823 t
ORIGIN      
        1 caccttttgc cgcatgccgc cggacaactg atggggatag cagtcggcca caagaggctc
       61 aaggcccacc tgctccagca aatcgctcac ccttctaccg gcttcctttc ccttcatgtt
      121 taggtgacgg gcgatgcact ccaatatctg ctctcttacg gtaataaccg gatttagcgt
      181 ctcaaggccg ttctgaaaca ccatagctat gtgcttccag cggtactcat tcagctgtgc
      241 tggagaaagc tccagtagat ttactccatt gtaggtgatt ctaccctcta cttttgcagc
      301 agaattcaat atgcccatta tggcaagacc caaggtagtt ttgccgcttc cagactcccc
      361 gataattccc agagcttgac cgggttcaag gacaagtgaa gtctccttta cagctggcac
      421 ctttgtattg tcctggcaat atgtaacgga gagattatcc actttaagca tggcaatgcc
      481 cctctttcat caaaaaactt tagctctatt gaccggcaat ttttatgtcc aacactcttt
      541 ccagttccct gcttaaggtt gccgtcgaaa ggacaagaat gattataaag gctaagggat
      601 ataccaccca ccacttccaa tagtcggtaa aatatattcc tcgaaaattc aaagcgaaat
      661 ttaagatgag cccccagctc ttgctcgttg ggtcaccgag gccaagaaag gacaagctcg
      721 cctcggacac tatggccctg cttatgagct ttatgaagct tgtggcacag atgggaaaga
      781 tttggggcat gaagtgctta aaggtaatgt aggaaaaact tgccccgtat cccttggcca
      841 ctttaatata gctttcctct tttaaagcca agacctttga gcgtattatt ctggccggca
      901 tgacccaaga caaaagggac aggattatta tgatgttttt tgtgctgggt ccaaagaagg
      961 cccctgcgac aatcataatt ggcaggctgg gtatggccat taatatgtct atcgcagcgg
     1021 tgacaataag gtccgttatg ccgccaaaat atcccgatac gatgcccaaa aaaacaccga
     1081 tcaagctgga cagcaatgcc gtcccgattc cgaccaagat gctgtttctg gcaccgtagc
     1141 atatttgtgc ccataggtcg atgcctaaat catcggtgcc caatatgtgt tccctgtttg
     1201 gtgcctgtaa agctggccct gaaggagtgt cccagggatg tctcacaagc tggggtgcaa
     1261 atactgccat aaagcacagg gcaaggatta ggaggacaga tattttgccc gctattgaaa
     1321 atccttttaa tatgtgaagc atcatctatc accgtcaaac tatcctggga tccaacttct
     1381 tatatataat gtctgccgca taattcataa taagcacaaa tagggtcacg gttaggaata
     1441 ttccttgaat caggggataa tcccttacca acaccgcctg ccgcatcaag agccccaagc
     1501 cggggtattt aaaaacattt tcaaccagta ttgtgcctcc tactgcctgg cctatactat
     1561 tgaacatcct ggtgacaatg ggcaaagccg catttgacaa ggcatgtcga aagagtatcc
     1621 gcgccgtgcc tattcctttg gccctggcag ttgtcatgta atccttgttc atcacagtga
     1681 tcatgctgtt cctggcaatg aggaaaaatc ctcccatctg aacagcggaa agggtaaggg
     1741 ccggtaaaaa tccatgatgc actatatcaa gtagccgctc catccatgta gcgtaatcgg
     1801 caaagggggt cattcctccc gaaaggggaa acaagccctg ctgggctgcc aggtagaata
     1861 aaaacaggat tcctatcaaa aatggcggta tctctgacac aaagaccatc gtagaataaa
     1921 ggatgttgtc tatggggcct tttcgatgcc aagcagacag gcagcccaaa atcgttccgg
     1981 caaagccccc gattattacg gacaatagca ccagggaaac tgaccagggg atgcggtttt
     2041 ttatgatcgt ccatacatca ttgctaaaat atatgctata tccaaggtct ccccttgcca
     2101 ggtttacaag atagcggccg tattggacga gaacgggctt atctaaaccg tagtaagcct
     2161 tctgcctttg gatttcttcg tccgaataac taacggttat ttccccctgt tcaccggata
     2221 aaaagtagaa ggggtctccc ggcatgagcc tgggcaggga aaaattcaag gtgattattg
     2281 tgaaaaaggt caagaatagc tttaggattt ttttgttaaa catggtcatc tttccaggta
     2341 cgataatttg ttgtgagttg tttcatggtg gtcaaataca tggacccagc catcgtactt
     2401 ttccggttta aataccgtat attcgacagt attgtaaagg ggtaataggg gtatttcatc
     2461 ggaaatgagc ttttgcagtt ccattatcat ggactttctg ctctcctcat ccagctcagt
     2521 gagctgtttt ttgcaaagct cggttatccg gagattgcta taaccgggaa taacattgga
     2581 tgcagggctg ttgtcccttg caacggtgac accgtagttt tccctgagga tatccgcatc
     2641 ctggccccat ccaccgtggc cgattagggc aagttcgtag ttttgttctt ttaccctgct
     2701 gtcccgggtc ttcatgtcag tgctttgaac cgaaagattg attccagcct tggccaggtt
     2761 tattttcaac agttctgcaa tcctggcttc catctggtca tctgctatta gcagtgttaa
     2821 atccaaggtc ctgtcgttta agaactcttt tgccttctct atgtcaaatt catattttgt
     2881 cacatcgggg ttataccata tgtggtctac ggggagatag cccgggcttg ccggaacggc
     2941 agcacctcta gcaaccttct cgatgagttc atctatatct atggcgtggg ctatggcctt
     3001 tctca
//
LOCUS       DAONUO010000263         2998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_22502_length_2998_cov_3.524635, whole genome shotgun sequence.
ACCESSION   DAONUO010000263 DAONUO010000000
VERSION     DAONUO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22502_length_2998_cov_3.524635"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK768_08845"
     CDS             complement(<1..186)
                     /locus_tag="PK768_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61117.1"
                     /translation="MLHILEFLTASKGYTLLFRLCFRHDHWLNCRYLIGEMDQINGID
                     VALARLLGYLEAYDKSGA"
     gene            253..327
                     /locus_tag="PK768_08850"
     tRNA            253..327
                     /locus_tag="PK768_08850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:285..287,aa:Thr,seq:cgt)
     gene            complement(530..1537)
                     /locus_tag="PK768_08855"
     CDS             complement(530..1537)
                     /locus_tag="PK768_08855"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HQA61118.1"
                     /translation="MKFKKLIAGILSAAVITFSAGTAYAVYRDEPISPEEMGEIIPIK
                     AELEEQQQIRYGSFTGTVKEIRDYEPIEGGKFISVENSEGEPANVIISDDTYVLDNAE
                     IKVGATITAYYDATKPMIMIYPPQYSAEVVVVEREDRHVKFDILDENLVSMDGALKLI
                     ISDETEIYMQDGTVYEGELANKKLVVIYTVAMESYPVQTRPERIIVLAEKAEDMPLFD
                     FSTMEIVVNNEVIEAPEAYTNDQGTVMVPLRAIAEALGYKVAWHNDTRTVTLGDDISL
                     TIGKDLYTRAGSSMRLGTAPELKGDRTFVPLSFFREVIGMNNAYIHAGQIIIDNEEKM
                     L"
     gene            complement(1613..2824)
                     /gene="nagZ"
                     /locus_tag="PK768_08860"
     CDS             complement(1613..2824)
                     /gene="nagZ"
                     /locus_tag="PK768_08860"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HQA61119.1"
                     /translation="MKKILLITIIIAILMCACVNKNDDNNKEQPPKAPEVEEIDPLKE
                     QIEKMSIDEKIGQLVMVGLDGYELDDSALDMIDKYKVGGFILFKRNIQSAAQTLELIN
                     SLKEANEENKIPLFIAVDEEGGSVSRMPEEFIKLPTSRAVGKVNSEEFAFEIGNVIGE
                     QIKSLGFNMNFAPVLDIDSNPNNPVIGDRSFGANPEIVSSLGTAVMKGLKSHVISTVK
                     HFPGHGDTSVDSHVGLPVVNHDIDRLKSFELVPFNMAIENGADAVMIAHILLPEIDEQ
                     NPATLSKVLITDVLRHEMEFDGVVITDDMTMGAIVENYDIGEAAVKSVLAGADIILVC
                     HDNEKQIKVLEALKQAATHGAIPESSLDEHVYRVIRLKQSYDLKDEEMEAVDVNAINE
                     KIKGMMNKYYK"
BASE COUNT          738 a          732 c          576 g          952 t
ORIGIN      
        1 agcgccgctt ttgtcatatg cctccaaata gccaagtagc ctggccaggg ccacatcgat
       61 gccgtttatc tggtccattt cacctatgag gtaacggcaa ttgagccaat ggtcatgcct
      121 aaaacagagt cgaaaaagta aggtgtatcc tttagatgca gtaaggaact ctagaatgtg
      181 aagcaagaat taattcttat tcgggtgaat ttgttgacta aaagccttaa acattgtaaa
      241 atatataggc aggctgatgt ggctcagtgg tagagcagcg cactcgtaat gcgcaggtcg
      301 ccggttcgaa tccggccatc agctccactt gaaaatgaag cttccggaat tcttccggaa
      361 gcttttttgc ttgtgcggct acaattctta cccggcccct attttttatc caacaatgtt
      421 tgttgcttgt ccagccctcg tcacttgcta ggatttgtgc tttgcaaaca aattgcaaac
      481 accaaaccat cattcacaat taaaagaggc cctttcgggc cacttcaact taaagcattt
      541 tctcttcatt gtcgattatt atttgtcctg catgaatata ggcattgttc atcccgataa
      601 cttctctaaa gaagctcaag gggacaaagg ttctatctcc tttaagttcc ggagcggtgc
      661 ccaagcgcat tgagctacct gcccttgtat aaaggtcttt accgattgtc agggagatgt
      721 catcaccaag ggtaacggtc cttgtgtcat tgtgccaggc gaccttgtat cccaaggcct
      781 ctgcaatggc ccgcagggga accatgactg tgccctgatc gtttgtataa gcttcaggtg
      841 cttctatcac ttcattgttt accactattt ccattgtgga aaaatcaaag agcggcatat
      901 cttcggcttt ttcagctaaa actatgattc tctccggtct tgtttgaacc ggatagcttt
      961 ccattgcgac agtgtagatc acaacgagtt ttttgtttgc tagctcacct tcataaactg
     1021 tcccgtcctg catgtaaatc tcagtttcat cggaaatgat aagctttaaa gcgccatcca
     1081 tgctgaccaa attttcatct aaaatgtcga attttacatg cctgtcttct ctttcaacta
     1141 ccacaacctc tgcactgtat tggggcgggt aaatcataat catgggcttt gtggcatcgt
     1201 agtatgccgt aatggttgcc ccaactttta tttccgcatt gtcaaggacg tacgtgtcat
     1261 cagatatgat gacatttgcc ggttctcctt ccgaattttc aactgatata aatttgccgc
     1321 cttctatagg ttcataatcc cttatttctt taaccgttcc cgtaaaagaa ccgtacctga
     1381 tttgctgctg ctcctctaat tccgctttta tcggaattat ttcccccatt tcctccggcg
     1441 atatgggctc gtcacggtac actgcataag ccgtccctgc tgaaaatgtg ataacggccg
     1501 ctgataaaat tcctgcaata agtttcttaa atttcatagc aaatctcctc cttcagtagt
     1561 tggtgttcta cctttttgga cggctattaa tgaaatatgt tccccatgta atctatttgt
     1621 aatatttgtt catcatacct tttatttttt cgtttatggc atttacatct accgcttcca
     1681 tctcttcatc cttcaaatca tagctctgtt ttaacctaat caccctgtac acatgctcat
     1741 ccaatgaact ttcgggaatt gcaccatgag ttgccgcctg ctttagtgcc tccaggactt
     1801 ttatctgctt ttcattgtcg tgacatacca ggattatatc ggcaccggcc agcacggatt
     1861 ttactgcagc ttcgccgatg tcataattct ctactatggc ccccattgtc atatcatcgg
     1921 ttatcaccac accgtcaaat tccatttcat gtcgcaaaac atctgtaatc aagactttag
     1981 acaaagttgc ggggttttgc tcgtctattt cgggcagcaa tatgtgggct atcattaccg
     2041 catccgcgcc gttttcgatg gccatattaa aaggaaccaa ctcaaagctt ttcagcctgt
     2101 caatgtcgtg atttaccaca ggtaaaccta catgagagtc taccgatgta tcgccatggc
     2161 caggaaagtg cttgaccgta gaaatcacgt gggactttag ccccttcata acggccgtgc
     2221 ctaggctgct tactatttcc ggatttgccc caaaagacct gtcgccgatt acaggattat
     2281 ttggattgct gtctatatcc agcaccggtg caaaattcat gttaaagccc agggatttta
     2341 tctgctcgcc tataacattg ccaatctcaa aggcaaattc ttcgctgttt actttcccga
     2401 cagccctgct ggttggcagt tttataaatt cttcgggcat tcggctcaca cttccccctt
     2461 cttcatcaac ggcaataaac aggggaatct tgttttcctc gttggcctct tttagggaat
     2521 tgatgagctc taatgtttga gcggcacttt gtatgtttcg cttaaataag ataaagcctc
     2581 ccaccttgta tttgtctatc atgtcaaggg cggaatcatc taactcgtat ccgtcaaggc
     2641 cgaccataac aagctggccg attttttcat ctatgctcat cttttcaatc tgttccttca
     2701 aggggtcaat ttcttccact tcaggagctt taggaggctg ttctttattg ttatcgtcgt
     2761 ttttattgac acaagcgcac atcaatatcg ctatgattat tgtgataagc agtattttct
     2821 tcattaatca agtctccttg ttataaagtt tgtttctaat aaattatacc acagccattg
     2881 gccagccaaa aaccttaccc gaattacgta attggtatta aagtgcggaa catttcactg
     2941 acaaatcgag taggaggggg tggctaaccc ccgtcctctc acaccaccgt acgtaccg
//
LOCUS       DAONUO010000264         2987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_22614_length_2987_cov_3.716235, whole genome shotgun sequence.
ACCESSION   DAONUO010000264 DAONUO010000000
VERSION     DAONUO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22614_length_2987_cov_3.716235"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="PK768_08865"
     CDS             complement(<1..1227)
                     /locus_tag="PK768_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQA61120.1"
                     /translation="MDTSIVVSLHVLDWIVIAIYVVIILYIGYYFSKRSKGFDDYFMA
                     GRDLNAPLLVGTLVSTFYGLDTLFGDSEVAFFEGISSFFAYALPYTALYAVMAFLSPV
                     FKEKFPEGTTMQEIAFKRYGKTAGVASSIASFLYSTNTMEMMGIGFLLRLTTGMPFWV
                     GTLIGAIFVTAFTWMGGLWAVTITDFIQFLIMMVTIGLALVIGWTAMGGYENIMVGLE
                     NFVGEDAEYYFQIGAGYLTPWLLLVYSVTAFAVLAEPAFFQRIFASAGPKEIKKAFTA
                     GIPMWLAFDWAVTFLGIMGAAAIGLAIIPEVVPNEALFAVAGQYLPAGVLGLLIAGVF
                     SAAMSTADSYFLVAGGVIGYDIYKGVINPDADQKQTERMTKIGVLISAALSLALAFFF
                     ERILEVWVFQATVIITT"
     gene            complement(1698..1970)
                     /locus_tag="PK768_08870"
     CDS             complement(1698..1970)
                     /locus_tag="PK768_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HQA61121.1"
                     /translation="MEMSTEIGILLAYGFGILVLYILGYLLLVPLKFLLKLIGNSILG
                     GLFILAINWVGGHWDLHIPLNFFSALFVGILGLPGAILLLILNNFL"
     gene            2135..2353
                     /locus_tag="PK768_08875"
     CDS             2135..2353
                     /locus_tag="PK768_08875"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HQA61122.1"
                     /translation="MYRHFLNLPHNQTEEEKLYASIRDAEIQLKLAEKRFNEVTDPDL
                     IDYAAYDIMAARSRYEYLLKMAKRQMGV"
     gene            complement(2354..2965)
                     /gene="recR"
                     /locus_tag="PK768_08880"
     CDS             complement(2354..2965)
                     /gene="recR"
                     /locus_tag="PK768_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQA61123.1"
                     /translation="MSGHMKYYAKPLTNLINQLSKLPGIGGKTAQRLAFHILSMDEKD
                     AIAIADAIVEGKRFMKYCSVCGNLTDQDPCSICSDATRDRKVVCVVESAKDVAAMERI
                     REYNGLYHVLHGAISPMEGIGPEDINLKSLIVRLRENDIDEVILATNPNIEGEATAMY
                     IARLIKPSGIKTTRIAHGIPVGGDLEYTDEITLTKAIEGRREL"
BASE COUNT          909 a          685 c          582 g          811 t
ORIGIN      
        1 tgttgtaata attaccgttg cctggaatac ccatacttcc agtatgcgtt caaagaagaa
       61 agctaatgcg agagataatg ctgcagaaat caacacaccg attttggtca ttctctcagt
      121 ctgtttttga tccgcatcag gattgataac tcctttgtaa atatcgtatc cgataactcc
      181 gccggctacc aggaagtagg agtccgccgt tgacatggcc gcggagaaga ctccggcgat
      241 gagcagccct aatactcctg caggcaggta ttgtccggct actgcgaaca atgcttcgtt
      301 aggcactact tcagggataa ttgcaagtcc gattgcagct gctcccatga ttcccaagaa
      361 tgttacagcc cagtcaaagg caagccacat gggaatacct gctgtaaaag cttttttgat
      421 ctctttcggt ccggccgagg cgaaaattct ctggaagaat gcgggctccg ccaatactgc
      481 aaacgcggtg accgaatata caagcaagag ccagggagtg agataacctg ctcctatctg
      541 gaaatagtat tctgcgtctt caccgacaaa gttttcgagt cctaccatga tattttcata
      601 gccgcccatg gcagtccagc cgataaccag tgcaagacct atggtgacca tcattattag
      661 aaactgaatg aagtcagtaa tcgttacagc ccaaagacca cccatccaag taaatgcggt
      721 gacaaaaatg gctcctatca aagttcctac ccagaaaggc ataccggtgg tcaggcgaag
      781 caggaatcca attcccatca tttccatagt atttgtggaa tataaaaatg atgctataga
      841 tgaagccacc cctgcagttt ttccgtatct tttaaaagca atttcctgca tcgttgtacc
      901 ttcaggaaat ttctccttaa aaactggcga taaaaatgcc atgaccgcat ataacgcggt
      961 gtaaggaagc gcatatgcaa agaatgatga aatcccttca aagaaagcga cttctgaatc
     1021 accgaacaat gtatcgaggc catagaaggt agaaaccaga gttcctacaa gaagaggtgc
     1081 gttaagatct cttcctgcca tgaagtaatc atcaaaacct ttggatcttt tggaaaaata
     1141 atagcctatg tacagaataa taacgacgta gatagctata actatccagt ccaacacatg
     1201 gagtgataca acaatactag tatccaacga catcccctcc ttttaagtat tcctgctcct
     1261 tatgcctgtg actgtaagaa gttatttgcc attctcgttc ccgtcaacgc ttatagtcgg
     1321 tgttcctgta ccatttcctc catcatttga actgttttaa tttcatgttt ccctacatta
     1381 ttaactgcgc aaacaaaaca aacattaaac gaaagcaatt ccaatatata gataatttga
     1441 taattaattt tatataattt tcagaaaata tgcaacttca tatttgcgat attataacta
     1501 ttaatttgct atacttaggt ggtatcagca tttaattcga atgcaaagaa tgtttgaaac
     1561 tcgaaagagg acatggctca gtttcagaac ttaattgtat atattagacc ttaaaaatag
     1621 acaggttctt atttgtagtt tttattatcg tgctgacggt agacacaatt cattattagt
     1681 ttcatctcgc cgctacgtta cagaaaatta ttcaatatca gcaacagaat tgcaccggga
     1741 agtcctaaaa tacccacaaa gagcgccgaa aagaagttca gcggaatatg aagatcccaa
     1801 tgtccgccga cccaattgat agctaaaata aacaacccgc ctaatatgct gtttcctatc
     1861 agtttaagca gaaatttgag aggtaccaga agaagatagc ccaaaatata caagaccagg
     1921 atgccaaaac catacgcaag caaaatgccg atttccgtgc tcatttccat aatctattca
     1981 cctcttgaga acagttctgt ccatattttg tataaaatat gctgaatatg catagaatag
     2041 aattgaataa caatagaagt ttataacaaa agaggaaagg atctgtgagg ttatggatag
     2101 tagttttatc ggagtgaagg gagttgcaga taaaatgtat cgacactttc tgaacctgcc
     2161 ccacaatcag accgaagaag aaaaactcta cgcatccatt cgcgatgccg aaatccagtt
     2221 gaagctggca gaaaagcgtt tcaacgaagt tacagatccg gatctgatcg attacgccgc
     2281 atacgatatt atggcggcga gatccagata tgaatatctt cttaaaatgg caaaaaggca
     2341 gatgggagta tgatcataat tcccgtctgc cttcgattgc tttcgtcaat gtgatttcat
     2401 ccgtatattc cagatctcct cctaccggaa tcccgtgggc tatacgggtt gtctttatgc
     2461 ccgacggttt gatgagtcgg gctatataca tggccgtagc ctcgccctct atattgggat
     2521 tcgtcgccaa gatcacttca tcaatatcat tttccctcag tcgaacaatc aatgatttta
     2581 aatttatgtc ctccggccca attccttcca tcggggaaat tgctccgtga agcacatgat
     2641 aaagcccgtt atattcgcga atgcgttcca tagcagccac atcctttgcg ctttccacca
     2701 cgcataccac ctttcggtcc ctggtcgcat cggaacagat actgcatgga tcctgatccg
     2761 taagattccc gcagacggaa cagtatttca tgaaacgctt cccttccacg atagcatctg
     2821 ctatcgctat tgcgtccttc tcatccatgg acaaaatatg aaaagccagc cgctgggccg
     2881 tctttccacc gatacccggc aacttggaga gttgattaat caagttggtc aaaggcttcg
     2941 cataatactt catgtgcccg gacatctcct ctctatagtc cagggat
//
LOCUS       DAONUO010000265         2987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_22619_length_2987_cov_2.585607, whole genome shotgun sequence.
ACCESSION   DAONUO010000265 DAONUO010000000
VERSION     DAONUO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22619_length_2987_cov_2.585607"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PK768_08885"
     CDS             complement(<1..597)
                     /locus_tag="PK768_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQA61124.1"
                     /translation="MENLVIWAPIMGILALAFAYMLAARITRSPAGNQRMQEISDAIH
                     EGAMAFLFREYKILSAFVAIMFIVIGLFIDWPTSISYLMGSIASVLAGFIGMNVATRS
                     NVRTAHAAQESQSKALSIAFSGGAVMGMSVVGLGLLGLGILYFLFGNPQDVKSFDVIN
                     GFALGASSIALFARVGGGIYTKAADVGADLVGKVEAGIP"
     gene            complement(623..850)
                     /gene="secG"
                     /locus_tag="PK768_08890"
     CDS             complement(623..850)
                     /gene="secG"
                     /locus_tag="PK768_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQA61125.1"
                     /translation="MELFLTIVYILLCVSLIISVLLQSGKSAGLSGSIAGGAESLFGK
                     KKGIDDKLARYTQILAFAFLAMSVIMIVFLR"
     gene            complement(1073..>2987)
                     /locus_tag="PK768_08895"
     CDS             complement(1073..>2987)
                     /locus_tag="PK768_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQA61126.1"
                     /translation="RCHQVTRNICFITPESSGNMNERRKLEGEFIARKILQMMTDDDV
                     KIFDKDSQEHRKAKFRDFAVLLRRRTHLKHYINAFKKHCIPFHVADTGSLMESPGVKN
                     LLYALNTIEFKDNINLYGVLSYLFNISDDCLAEFVLKSGDLSSGLKEDVDLSAASEKL
                     VKAFQLIRSWCKIKDKLTIRELAGAIVEDTGLLPSLAKIDFVEIESLYKFMDLCWHYD
                     RQGYTLKEFMDEIADFGDQQQEATDVSEEEDVVKFITIHSSKGLEFPIVILADSGQNI
                     SFSSSEILFDPEKGFALKKDKNKWEGLKEHLEEREMEEAKRLLYVALTRARDYLVISG
                     EMASEKKESFLKWLNQESNAIQEMSIDVISDLSELLVDEIKLPASEKAEAACAKVQAQ
                     VGHIPVHRRSSVTSLGEFLRCPRRYYFSSVLGIPEKIFYKRERQVPGLSAVEKGLIVH
                     ELIEKLYKDKLEEIDVDDFLSQQGYSFGLEDITFIKKCLENFVSSGIYNIPGEVFSEI
                     PFAYRVNSRWLLTGKADLLVFEDDGATIVDFKTNTQIHPNLLEIYRLQVLTYALAISN
                     IYNVEPKRAIVASLFEGNFTQIEMSPSILAECNEQILCILDRIENGAFQDKARDAVTC
                     NFCAYKNMLCKLL"
BASE COUNT          867 a          702 c          507 g          911 t
ORIGIN      
        1 aggaatacct gcttctactt tacctaccag atcagcacct acgtcggcag ccttggtata
       61 tatacctccg ccaaccctcg caaaaagcgc tatagagctt gcgcctaagg caaagccgtt
      121 tatcacatca aaactcttga catcttgggg atttccaaat aaaaaataga gaatcccaag
      181 tcctaaaagg cctaaaccca ccaccgacat tcccatgacc gctccacctg aaaaggctat
      241 gctgagagcc ttgctctggc tctcctgagc cgcatgggct gtcctcacat tggaccttgt
      301 tgccacattc atgccaataa aaccggcaag cacagaggca atgcttccca tcaggtagct
      361 tatggatgtg ggccagtcaa taaacaagcc tataactata aacattatgg caacaaatgc
      421 tgacaagatt ttatactcac gaaaaagaaa agccatagct ccttcatgta tagcatcgga
      481 tatctcctgc atcctctgat tgcctgcagg actcctagtt atccttgcag ccaacatata
      541 agcaaaagct aaggccaaaa ttcccataat tggggcccat atcaccaggt tctccattga
      601 cacttccctc cagctttaag tcctaacgta aaaaaacaat cattatcact gacattgcga
      661 gaaaagcaaa agccaaaatc tgtgtgtacc tagccagctt atcatctatg ccttttttct
      721 tgccaaacaa actctcagca ccaccggcaa tgcttcccga aagaccagca gatttgcctg
      781 actgcagcag tacagaaatt ataaggctta cacaaagtaa tatataaaca atcgtcaaaa
      841 acagttccat ttatgatacc tccgtgcatc tcatagtggt aattatagca caacactttt
      901 ttaaagtaaa gcattttgcc tgtaattgtg ctacactatt aaagttattt taaattatac
      961 tgttacatta tatgattacg cgtggagaaa agcttgtttc ttcataatat ttgcctgatg
     1021 atatgttccc aattttgttg tgagatttcg gatttgctta ccaccttctt tactatagta
     1081 atttacacaa catgttttta tatgcacaaa aattacatgt tacagcatct cgggccttat
     1141 cttgaaatgc accattttct atcctatcta ggatgcataa gatttgctca ttgcactctg
     1201 ccaggatgga tgggctcatc tctatctgag taaaattccc ttcgaacagt gatgctacaa
     1261 ttgccctttt aggctccaca ttgtaaatat ttgatattgc caatgcatat gttaacactt
     1321 gcaaccggta gatttcaagt aaatttggat ggatttgcgt atttgtttta aaatctacaa
     1381 tagttgcacc atcatcttca aagaccaaga gatcagcttt tcccgttaaa agccatcggc
     1441 tatttacacg ataagcaaaa ggaatctcgg aaaaaacttc tcccggtata ttatatattc
     1501 cgcttgaaac aaagttttct agacactttt taataaaagt tatatcttca agaccaaatg
     1561 aatatccctg ttgcgaaaga aaatcgtcta catctatttc ctcaagttta tccttatata
     1621 gtttttctat gagctcatgg actatcaagc ctttttcgac ggcagaaagc cctggcactt
     1681 gcctttctct cttataaaaa atcttctcag gtatgcctag aacactgcta aagtaatacc
     1741 tcctggggca tcttaggaat tctcccaatg aggttacgga agatctccta tggaccggaa
     1801 tatgaccgac ttgggcttgg acttttgcac aagcagcttc agctttttca gaagcaggta
     1861 atttgatctc atccactagc agttctgata gatccgaaat tacatctata gacatttcct
     1921 gaatggcatt tgattcttga tttaaccatt ttaaaaagct ttcttttttc tcggaggcca
     1981 tttctcccga tatcacaaga taatccctgg ccctcgtaag ggctacatag agcaaccgtt
     2041 tggcctcttc catctccctt tcctctaaat gttcttttag tccttcccac ttattcttgt
     2101 cctttttcaa ggcaaatccc ttttcagggt caaaaaggat ttccgaagaa ctaaaagata
     2161 tattctgacc gctgtccgcc aggattacta tgggaaactc caagcccttg gaagaatgga
     2221 tggtgataaa ctttactaca tcctcttctt ccgaaacatc ggtggcctcc tgctgctggt
     2281 caccaaagtc agcaatctcg tccataaact cttttaaagt gtaaccctgc cggtcgtaat
     2341 gccagcaaag atccataaac ttatatagac tttcgatctc tacaaagtca attttagcta
     2401 aagagggcaa gaggccggta tcctctacaa ttgcccctgc aagttcccta atcgtaagct
     2461 tgtcttttat tttacaccag cttcggatta attgaaaggc tttaaccagc ttttctgaag
     2521 cagcggacaa gtctacatct tccttcagtc ctgatgataa atcgccgctc ttgagcacga
     2581 attctgcaag gcagtcgtca cttatattaa agagatatga aagaactccg tacaaattaa
     2641 tattgtcctt gaattcaatg gtatttaaag cgtagagaag atttttcact cccggacttt
     2701 ccataaggct tcctgtgtct gccacatgaa atggaataca gtgttttttg aaggcattaa
     2761 tatagtgttt aagatgggtt ctccttctaa gtagcactgc aaaatcccta aacttagctt
     2821 tgcggtgctc ttggctgtct ttgtcaaaaa ttttaacgtc atcatcagtc atcatctgca
     2881 gtattttcct ggcaataaat tcgccttcca acttccttcg ctcattcatg ttgccgctgc
     2941 tttcaggtgt gataaagcaa atattcctgg tgacttgatg acatctg
//
LOCUS       DAONUO010000266         2973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_22743_length_2973_cov_3.481494, whole genome shotgun sequence.
ACCESSION   DAONUO010000266 DAONUO010000000
VERSION     DAONUO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22743_length_2973_cov_3.481494"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /gene="ftsZ"
                     /locus_tag="PK768_08900"
     CDS             complement(<1..943)
                     /gene="ftsZ"
                     /locus_tag="PK768_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQA61127.1"
                     /translation="MLDFDVEVEQFANIKVVGIGGGGNNAINRMIDSGLKGVEFIAVN
                     TDAQALYLSKADKKIQIGEKLTKGLGAGANPEIGRKAAEESKDIMEEVLQGADMIFIT
                     AGMGGGTGTGASPIVAEISKSLGILTVGVVTKPFTFEGKKRQANAEIGIADIKNNVDT
                     LITIPNDRLLSIAEKKTSMIEAFKMADDVLRQGVQGISDLIAVPGLINLDFADVRTIM
                     LDTGLAHMGIGKGSGEGRAVEAAKQAISSPLLETSIEGAKGVLLNITGGANLGLLEIN
                     EAAELISSVADPEANIIFGAVIDEKLQDEIIITVIATG"
     gene            complement(961..2178)
                     /gene="ftsA"
                     /locus_tag="PK768_08905"
     CDS             complement(961..2178)
                     /gene="ftsA"
                     /locus_tag="PK768_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQA61128.1"
                     /translation="MGKGNIIVSLDLGTSKTCCMVGGITPSGDIDVLGYGISETSGMK
                     KGTIINIDLVVQSIIKSVELAEQMSNTKIKNVAVGLSGGNISLLSNRGVVAIPRNDRE
                     INHQDVERVIQAAKIMAIPYDKEIIDVIPREFIVDGCDGIKDPVGMIGTRLEVSACIV
                     VGLLTAIQNIARCVQKAGLSVESMVLKPLAAAEMLLTDDEMNMGVVLVDVGAGTTEIA
                     VFQEGCITAYEMIPVGGNLITNDIAIGLRLPYAQAEEIKCKYACAVSSLASEKADIEI
                     QSLGDSAPRKISQRELTAIIEPRVQEILSYIISTIAGFNLKTMLPAGIVFSGGGLVHI
                     KGFLDMAQQLLDFPVRIGTTDSYGKEQTFTVALGLLNYIIKHRALYVNLTMKERSASG
                     FFERAKRILREYF"
     gene            complement(2256..2621)
                     /locus_tag="PK768_08910"
     CDS             complement(2256..2621)
                     /locus_tag="PK768_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HQA61129.1"
                     /translation="MKSRVIYALIGLLVGLFIGYYSPITVPTIYAPYVSISILAALDS
                     VFGGIKALEEESFDINIFITGFFSNALMAGLLAYFGDRLGIQLYLAAIFAFGVRIFQN
                     LAIIRRSIIKRLFLKNEKK"
     gene            complement(2611..>2973)
                     /locus_tag="PK768_08915"
     CDS             complement(2611..>2973)
                     /locus_tag="PK768_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HQA61130.1"
                     /translation="DPNLDLIHDEDLLKVINELFAAGAEAVSVNGQRIITTSEIRCVG
                     PTIIINSVKLAPPFIIHAVGDPEILEAALKMRGGVIESLQVFGIQVSIKKQDKIEIPA
                     YTGPIQFKYLKPVKAGEK"
BASE COUNT          827 a          723 c          494 g          929 t
ORIGIN      
        1 agcctgtagc tattaccgta attattattt catcttgcag cttttcgtcg attaccgcac
       61 caaatattat gttggcttca gggtcagcaa ccgatgagat cagttcggca gcttcgttaa
      121 tttcaagcag gcccaaattc gcacctccag ttatatttaa gagtacacct ttagcccctt
      181 ctatggaagt ctcaagcaga ggactggata tggcttgttt tgccgcctca acggctctcc
      241 cctcacctga gcccttccct attcccatgt gtgcgagacc ggtatcaagc ataattgtcc
      301 tgacatctgc aaagtctaag tttattaaac ctggtacagc aattaaatct gaaattcctt
      361 gaacaccctg ccttaaaaca tcatcagcca tcttgaaagc ttcgatcatt gaagtcttct
      421 tttctgctat agatagcaat ctatcatttg gaatcgtaat tagagtatcc acattgtttt
      481 ttatatcggc tatccctatt tctgcattgg cctgacgttt tttcccctca aaggtgaaag
      541 gtttggtcac aactcctact gttaatatgc cgaggctctt agatatttcc gctactatag
      601 gcgatgcacc ggtgcctgtc ccaccgccca taccggcagt aataaaaatc atatctgcac
      661 cttgaaggac ttcttccatt atatctttgc tctcttccgc tgcctttctg ccgatttccg
      721 gattggcgcc tgcaccgaga cctttagtga gcttctcacc gatttgaatt tttttatcgg
      781 ccttggataa ataaagagcc tgtgcatcag tatttaccgc gataaactct actcctttta
      841 ggcctgagtc tatcattcta tttattgcgt tattgccacc tccgccgatt cctacgacct
      901 tgatatttgc aaattgttcc acctctacat caaaatcaag cacgttaata cctcccaaac
      961 ttaaaaatac tctcgcaaaa tcctctttgc tctttcaaaa aatccggaag ccgacctttc
     1021 tttcatggtt agatttacgt ataaagccct atgtttaata atgtaattta aaagccccaa
     1081 agccaccgta aaggtttgct ctttaccata tgaatcagtg gtgccaattc ttacgggaaa
     1141 atcaagtaat tgttgtgcca tatctaaaaa acccttgata tgtacaagac ctccaccaga
     1201 aaaaacaata ccagcaggca acatagtctt taaattaaac cccgctatcg tgctgatgat
     1261 ataagataaa atttcttgaa cacggggttc gataattgcg gtcaattccc tctgggatat
     1321 ttttcttgga gctgagtcac ctaaactctg aatctctata tctgcttttt cagaggctaa
     1381 actcgataca gcacacgcat atttgcattt tatctcctct gcctgtgcat aaggaagtct
     1441 gagacctata gcaatatcat tggtaatcaa attgccccct acgggaatca tctcgtaagc
     1501 agtgatacac ccttcttgaa aaactgcaat ttctgtagtt ccagccccta catcaaccaa
     1561 aactactccc atattcatct catcatcagt taacagcatt tccgctgcag ccaatggttt
     1621 taaaaccatt gattctacag ataagcccgc tttttgtaca cagcgggcaa tattttgtat
     1681 tgccgtaagg aggccaacca caatacaggc agaaacttct agtctcgtcc ctatcattcc
     1741 gaccggatcc tttatgccat cacaaccatc aacaataaac tctcgtggta ttacatcaat
     1801 aatttcctta tcgtagggaa ttgccataat cttggcagcc tgaatcactc tttccacatc
     1861 ttgatgattg atctccctgt cgtttctggg aatggcaaca actccgcgat ttgatagcag
     1921 tgaaatattg cctccggaca atcctacggc aacattttta atttttgtat tcgacatttg
     1981 ttctgctaat tcaactgatt ttattatact ttgaacaacc aaatctatat ttatgatagt
     2041 gccttttttc attcccgatg tctcggaaat gccatatccc agtacatcta tatctcccga
     2101 gggagttatt ccaccaacca tacagcaggt tttagatgtc cccaggtcta aactgactat
     2161 gatgttgcct tttcccaaac ttacacctcc ccttgcctgt tgggcaggta gcttagcata
     2221 taataaaatt caacacatga attcgatttt cctttttatt ttttctcatt ttttaaaaat
     2281 agtcttttaa ttatacttcg gcgtattatg gcaagatttt ggaatatcct gactccaaaa
     2341 gcaaaaatgg cagcaaggta tagctgtatt cccaatctat cgccaaaata tgccaataga
     2401 cccgccatta aggcattgct gaaaaaaccc gttataaaaa tattaatgtc gaaactctcc
     2461 tcctctaagg ctttgatgcc gccaaagacc gaatcaaggg cagctaaaat tgaaatagag
     2521 acgtaaggag cgtaaatcgt tggcacagta attggtgaat agtagcctat aaataagcct
     2581 acaaggagtc ctatcaatgc atagatcact ctacttttca cctgccttca caggcttcaa
     2641 atatttaaac tgaattgggc ccgtatatgc tgggatttcg atcttatcct gttttttgat
     2701 actgacttga atgccgaaaa cctgaaggga ctctatgact cccccccgca tctttaaagc
     2761 tgcttccaat atttcaggat cccccacggc atgtattatg aatggaggtg caagtttgac
     2821 ggaattaata atgatagtcg gccctacaca ccttatttcg ctggtggtta ttattctctg
     2881 cccatttaca gatacagcct cggcaccagc tgcaaacaat tcatttatta ccttcaataa
     2941 atcttcatcg tggattaaat ccaaattcgg gtc
//
LOCUS       DAONUO010000267         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_22938_length_2951_cov_3.861878, whole genome shotgun sequence.
ACCESSION   DAONUO010000267 DAONUO010000000
VERSION     DAONUO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22938_length_2951_cov_3.861878"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..903)
                     /locus_tag="PK768_08920"
     CDS             complement(229..903)
                     /locus_tag="PK768_08920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQA61131.1"
                     /translation="MKITVIHGQKRYGSTWNTTKLLLDFLVSQDDEYNEFFVNDISNC
                     IGCFTCIMDDEEKCPHRSLTEPIIQTIEQSDIIIVATPNYCMGMTGQLKTFFDHMAYR
                     WMSHRPLDEMKNKVAIAVSTTAGAGAGVATKQIAKQLFWWGIPKVYRLNQAVAASKWE
                     DVKPKTKAKIKQKAMKIAKKVKKKHGKVDRGLRQKFIFKMMGAIQKSGLGTPKDASYW
                     KEHGWI"
     gene            complement(908..1516)
                     /locus_tag="PK768_08925"
     CDS             complement(908..1516)
                     /locus_tag="PK768_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA61132.1"
                     /translation="MTTKDLIFYTALRLFSERGYDGVSMRDIAAAVGIKAASIYNHFA
                     SKEDIFNSLLLEMKSRYEHMVSVVGVPNGSANESAQQYIGMPVEQLQMIAQGLFQYFA
                     KDEFAAPFRKILSSEQYRNSAAGDVFRSMFIDGALEYQTDLFKALIEQGEFIDADPEI
                     IALHFYSPIFLLLQSYDETQEQTIMQKLEKHVSQFSRLYVRR"
     gene            1840..2661
                     /locus_tag="PK768_08930"
     CDS             1840..2661
                     /locus_tag="PK768_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61133.1"
                     /translation="MKKTDKFGLFFLITLLPYSYILYRGFDAAINGIWFHFIMDTTQI
                     FGLSAFVYGMIETTIELVIFPILPICMIYQFVYLIYFCVRLTKKKASNIPLGVAAFIT
                     VALVAFLFGGRGISEYKEACDKERFVQKVAQNIYMLAEDADVIIEDYSSPVRITGGIA
                     GTEFVRNTILIDYDTKKVTFLFAREFITVESENMYYKEFLLTENNKIDSKNIHYTKEL
                     DEPGKTLTVFYLEEHKRKTVAIELCMEDGSVYSINNILDEGGQLGYFLHFSWSGV"
     gene            2724..>2951
                     /locus_tag="PK768_08935"
     CDS             2724..>2951
                     /locus_tag="PK768_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA61134.1"
                     /translation="MSKLFLTGIELDLEGHDDTHYPFNLPAVKHIGRFRFNKPVTFLV
                     GENGTGKSTILEAIAVKYGFNAEGGSRNFNFS"
BASE COUNT          929 a          497 c          537 g          988 t
ORIGIN      
        1 taggcagtga gggttaccta aaattaaatc taaattggaa agtttatctg atatttctta
       61 tagaattaat tgtctctttc attttaggaa tgaagcataa aaactttgag ttataaatga
      121 agtatcgctc actgggaaaa cagtaccgat aaaaagcgag gtacagagtg tccagccgac
      181 attcttgtat ctcgcttttt tcatatttat taatctaaat cggatttgct atatccagcc
      241 gtgttctttc cagtaagagg cgtccttagg cgttcccaaa ccagattttt gaatagcacc
      301 catcattttg aagataaact tctggcgcaa tccacgatca acctttccgt gttttttctt
      361 tactttttta gcaattttca ttgctttttg ttttattttt gcttttgttt taggctttac
      421 atcttcccat tttgaagccg ctacagcctg attgagccgg tataccttgg gaattcccca
      481 ccaaaacagt tgctttgcaa tctgctttgt agctacaccc gcaccggcac cggctgttgt
      541 cgatactgca attgctactt tgtttttcat ttcatctaat ggacgatgac tcatccagcg
      601 ataagccata tgatcaaaaa aagtttttaa ttgtccggtc atacccatac agtaatttgg
      661 ggttgcgaca attattatat cagattgttc aattgtctga atgataggct ctgttaagct
      721 tcgatgcgga catttttcct catcatccat aatacaggta aaacaaccaa tgcaatttga
      781 tatgtcattt acaaaaaact cattatattc atcatcctgg gaaacaagaa aatcaagcaa
      841 caacttagtt gtattccatg tgcttccata gcgcttttga ccgtgtataa cagtaatttt
      901 cattccacta cctcctaacg tacagtcttg aaaactgact aacatgcttt tctaattttt
      961 gcattattgt ttgctcttga gtttcatcgt aactttgaag taacaagaat ataggtgaat
     1021 agaagtgtaa cgcaataatc tcgggatcgg cgtcaataaa ttcaccttgc tctataagag
     1081 ctttaaaaag gtctgtttga tattccaatg caccatcaat aaacatactt ctaaaaacat
     1141 cacctgctgc agaattacga tactgctcag aagaaagtat cttacgaaag ggtgcggcaa
     1201 attcatcttt agcaaaatac tgaaaaagcc cttgtgcaat catctgtaat tgctctaccg
     1261 gcattccaat atattgttgt gcagattcgt tagcacttcc attagggaca ccaactacac
     1321 taaccatatg ttcatacctg cttttcattt ccaatagaag actattaaaa atatcctcct
     1381 tacttgcaaa atgattatat attgaagctg ctttgatacc tactgcggcc gctatatcac
     1441 gcatggaaac accatcataa ccacgttcgg aaaaaagtcg tagcgctgta taaaatatta
     1501 agtctttggt tgtcataaac atccctcgca atcaattaca cttactttta ggacaagctt
     1561 ttagtcaaat aaatattgtt taaataatag tttgatataa aaatatgtaa caaagccttc
     1621 actaacaagt gttagcatca ttataactaa cggtcgttag tttgtcaaga ggctattgta
     1681 ttattaaagc tgcgatttat agaaatagaa gcaaggtaaa aaaattgcaa tcgataaagg
     1741 tgtaacatcc aacagtgact tttcttgtag aaaaatattg atttactgcc tgatatttga
     1801 tattattgga atatatttca attaaactgg agattgtgta tgaaaaaaac tgataaattt
     1861 ggattgttct ttctaataac tttgctgcca tattcataca tactgtatcg tggttttgat
     1921 gctgcaataa atggtatatg gtttcatttt attatggata caacacagat attcggcttg
     1981 tcggcgtttg tatatggcat gattgaaaca actatagagc ttgtaatttt tccgatactg
     2041 cctatatgca tgatatatca atttgtgtat ttaatttact tctgtgtccg actaacaaag
     2101 aaaaaggcga gcaatatacc tttaggtgta gctgcattta taactgttgc tcttgtagct
     2161 tttctttttg gagggagagg gatttcggag tataaagaag catgtgataa ggaacggttt
     2221 gtacagaaag ttgcgcagaa tatctatatg cttgcagaag atgcggatgt aattatagag
     2281 gactattcaa gtccggtaag aataacaggc ggaatagcag gtacagagtt tgttcgaaac
     2341 accattttga ttgattatga tacaaagaaa gttacttttt tgtttgcaag ggaatttatc
     2401 acagtggaat cagaaaatat gtattacaaa gaatttttat tgactgagaa taataaaatt
     2461 gacagcaaga atatacatta tactaaggag ctagatgagc ctggcaaaac tttaacggtg
     2521 ttttatcttg aggagcataa aagaaaaact gtcgccattg aactttgcat ggaggatggt
     2581 tctgtatatt caataaacaa tattttagat gagggaggac aattgggata ttttcttcat
     2641 ttttcatggt cgggtgtata aaattttaaa attgtgtagt ttttttgaaa actatgtgat
     2701 aaaatacaat tgggtgataa tagttgagta agcttttttt aactggtatt gagctggatt
     2761 tggaaggtca tgatgacacc cactatcctt tcaatttgcc agccgtaaag cacattggaa
     2821 gattccgttt caacaaaccg gttaccttct tggtgggtga aaatgggacg ggaaaatcca
     2881 caatacttga agcaattgct gtaaaatatg ggtttaatgc agaaggggga tccaggaatt
     2941 ttaatttttc t
//
LOCUS       DAONUO010000268         2950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_22950_length_2950_cov_3.425216, whole genome shotgun sequence.
ACCESSION   DAONUO010000268 DAONUO010000000
VERSION     DAONUO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22950_length_2950_cov_3.425216"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PK768_08940"
     CDS             complement(<1..562)
                     /locus_tag="PK768_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HQA61135.1"
                     /translation="MKYPKLFEPAALGSLNLPNRIVLSPMHTNFTIGNKYTEKYIEYY
                     KERAKGGVGLIITAHVMAEVDVDPYPTTFGYPTIDSAGEVKYFAELTETVHKYGTKIA
                     IELSPGTGRLADEIINRKPPVGPSEIPLLMMPDVKTRELTKSEIKQLVKAYGRAAGLA
                     KQAGFDAIYVHFMAYLGDQFLSSCWNK"
     gene            792..1145
                     /locus_tag="PK768_08945"
     CDS             792..1145
                     /locus_tag="PK768_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61136.1"
                     /translation="MPTFANPFSGNVERKVSKQELIQAIRLDIAAELEAIFIYDAHIQ
                     ATDDVFVKRVLSDIRDEEKAHVGELMTLLRHLDPTEADFFLEGEEEVREMMEELGIKE
                     PPKPGSTVGSLLKEE"
     gene            1168..1980
                     /locus_tag="PK768_08950"
     CDS             1168..1980
                     /locus_tag="PK768_08950"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HQA61137.1"
                     /translation="MDFLARDTVDFSSDFWKEIDSTVVDTVKKNLIGRRFLSLHGPLG
                     AGATSIHIDNIDAVQEEKDGVVKTVGRRYVELPQLHEDFTIYWRDIEHSEMTGQPLDL
                     SKAIMAAQALAQKEDRLIFFGDEFLGLEGLFTAEGASKISRSDWTVGENAFTDVVSAI
                     AQFQSKSLIGRYSLILSPDMYYKLYRLQPSLGMTEEERIRKILNGHIYSSPVLGEGKA
                     VMVCAEPQYMDLAVGKDIETGYLETTDFNHVFRIVETVALRIKCKDAIIIFE"
     gene            complement(2167..2907)
                     /locus_tag="PK768_08955"
     CDS             complement(2167..2907)
                     /locus_tag="PK768_08955"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HQA61138.1"
                     /translation="MKKLFQGEEIVHGAKILGDMMKKSASTVVLTGAGMDTESNIPDF
                     RGKNGLWRNMNPMLVASIDNFYNNYDEFHDYYTTRIKRLEDVKPHEGHYILADFEKRG
                     IIKSIATQNVSGLHHMAGSNSVYELHGNIKRAVCNSCRREASLDDFLNKQKCQHCGDL
                     LRPAVVLFGEALPRDMWSLAEKDIKNCDLLIVIGTSLEVYPVNQFPRMARGRIALINN
                     EDLSYGREFDIKLIGRAKEVLQELSRNV"
BASE COUNT          766 a          688 c          662 g          834 t
ORIGIN      
        1 ttttattcca gcaggaagag agaaactggt cccccaaata ggccataaag tgaacgtaaa
       61 tcgcatcaaa gcctgcctgc ttggctaatc ctgcagctct tccgtaagcc tttaccagct
      121 gtttgatttc actttttgta agctcccttg tctttacatc cggcatcatc aacagcggta
      181 tttccgaagg tcctaccggc ggcttcctat ttatgatttc atctgccagc ctgcccgtgc
      241 ccggtgaaag ctctatggct attttcgtcc cgtacttgtg gacagtttct gtaagctctg
      301 caaagtactt tacttcgccg gccgaatcta tggttggata gccgaaggtg gtgggataag
      361 ggtccacatc tacttctgcc atcacatggg cggtgataat caggccaact ccgcccttcg
      421 ccctttcctt gtaatattca atgtattttt cagtatactt attgccgatg gtgaaattgg
      481 tgtgcatggg cgataataca atgcgatttg gcaaatttag cgaacccaaa gctgccggtt
      541 caaaaagttt cgggtacttc aatattacgt cctccttcgc attcggtgtg attttcactg
      601 tttattttcc ccattgtttc caaatataat cccgaagcaa ataattatcc tacggttaaa
      661 taagttaatc agttaggcgt ctggcaccaa gtttaccttg acagtaatga atactatttt
      721 atataatgga gatgaaaagt tcgtattttc actttattag gattgttaaa ataaaaaaag
      781 gagatggtgt catgcctacc tttgccaatc catttagcgg taatgtggag cgtaaagttt
      841 caaagcagga gctgattcaa gccatacgcc tagatattgc agcagagctg gaagcgattt
      901 ttatctatga tgcccacatt caggcaactg atgatgtgtt tgttaagagg gtgctcagcg
      961 atataaggga cgaagagaag gcacatgttg gcgaactgat gaccctactt cgccatttag
     1021 atcccacgga agctgatttc ttcttggaag gcgaagaaga ggtcagggaa atgatggaag
     1081 aactcggaat taaggaaccg ccaaagcccg gttcaaccgt tggcagttta ttaaaggaag
     1141 aataagttaa cggggaggat aagatatatg gattttcttg cgagggatac tgttgacttt
     1201 tcaagtgatt tttggaagga aatagactca acagtggtgg ataccgttaa gaaaaatcta
     1261 ataggtcgca gattcctgtc ccttcatggg cctttaggtg caggggcaac cagcatacat
     1321 atagacaata tcgatgccgt gcaggaggaa aaagacggcg tcgtaaagac agtaggacgt
     1381 cgctatgtag aactgcccca gctacatgag gatttcacca tatactggag agatatcgaa
     1441 cacagtgaaa tgactggcca gccgctggac ctttccaagg ccataatggc ggcacaggca
     1501 ttagcacaaa aggaagacag gctcatattc ttcggagatg agttcctggg cttggaaggc
     1561 ctcttcactg cagagggcgc ttccaagatt tcaaggtccg attggaccgt cggcgaaaat
     1621 gcctttaccg acgttgtttc agccatcgcc cagttccaga gcaagagcct tatcggccgg
     1681 tacagcctga ttttaagccc cgatatgtac tataagctat accgcctgca gccttcgctg
     1741 ggaatgacag aagaagagcg catcaggaaa atattgaatg gccatatata cagctcgccg
     1801 gtcttgggcg agggcaaggc agtcatggta tgcgccgagc ctcagtacat ggaccttgcc
     1861 gtgggtaagg acatagaaac cgggtacctg gaaaccacgg actttaacca tgtattccgc
     1921 atcgtagaaa ccgttgccct tcgcattaag tgcaaagacg caataatcat cttcgaataa
     1981 aacaagctcc tggacagggg cttcaaataa aacgccttcc ggtttttgcc tgaaggcgtt
     2041 ttgttttgaa atatgaataa ttggggacgg gtgaataatt ggggacgggt acaaaatatt
     2101 cactcgtgaa tattttgtac ccgtccccaa ttattcaccc gtccccaatt attcactttc
     2161 attcattcaa acatttctac ttagttcctg taagacttcc tttgcccttc ctatgagttt
     2221 tatatcaaat tcgcgaccat agcttaagtc ctcattgttt atcagtgcaa tcctacccct
     2281 ggccattctt ggaaattggt ttaccggata cacttcaagg ctggtcccta taactatcag
     2341 caagtcgcag ttcttgatat ctttttcggc caaagaccac atgtccctcg gcaaggcttc
     2401 gccgaacaat accacggcgg gcctcaagag gtctccgcaa tgctgacatt tttgcttgtt
     2461 caaaaagtca tccaggctgg cttcccgcct gcagctattg catactgccc ttttaatatt
     2521 gccgtgaagt tcgtagacac tattgctgcc tgccatatgg tgcaggcccg aaacgttctg
     2581 ggtagcgatg ctcttgataa tcccccgctt ttcaaagtcc gccaggatgt aatggccctc
     2641 gtgaggcttg acgtcctcca gcctttttat ccgcgtcgtg taataatcgt gaaactcatc
     2701 gtaattgttg tagaaattgt cgatactggc caccagcatg gggttcatgt ttctccagag
     2761 gccatttttt cctctaaaat ccggtatgtt gctttccgta tccatgccgg cacccgtcag
     2821 cactaccgtg cttgcggatt ttttcatcat atctcccaag attttagccc catgcacaat
     2881 ctcttcaccc tggaacagct ttttcatttt gttttcacct ctgaaagtta cttatctata
     2941 ttatatcagc
//
LOCUS       DAONUO010000269         2921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_23254_length_2921_cov_3.806350, whole genome shotgun sequence.
ACCESSION   DAONUO010000269 DAONUO010000000
VERSION     DAONUO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23254_length_2921_cov_3.806350"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..109
                     /locus_tag="PK768_08960"
     CDS             8..109
                     /locus_tag="PK768_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQA61139.1"
                     /translation="MSRIYTGMHYPSDVLSGAFLGMLCAIVTRLVIN"
     gene            287..1672
                     /gene="pepV"
                     /locus_tag="PK768_08965"
     CDS             287..1672
                     /gene="pepV"
                     /locus_tag="PK768_08965"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HQA61140.1"
                     /translation="MDIEQYITANEKKLIEATSEIIKIRSVKESPMQGMPFGEGPAKA
                     LNYALDLAKSFGLETKNLDNYAGWAQWGEGDEMIGILVHLDVVPEGSGWTYPPYGGEI
                     HDGRIYGRGAIDNKGPAMAALFALKSLKDAGIKFNRRVRVIFGTDEESGFECMEYYKK
                     HDEAPTMGFSPDANYPIINGEKGVLTFSFTTLLDAEKVDQRARLLSFKGGHRPNMVPD
                     YAEAHLTGDIDEITNAVKTALDIVPGNFEIEQANDEIVIKSYGLSAHGSTPERGKNAC
                     MLLAKLLGEVKLAKPYADFIGFLNDKIGLDTTGKGMGIDFKDDISGQLTFNVGIVGCD
                     DNKATVTVNIRYPIKYRGDEIILEIEKHIPDSIRIENISDNKPHYIPEDSIIIQKLKE
                     AYEKVTGEKAYCFSIGGGTYARMFDNCVAFGPVFPGKPELAHQTDEYIEVEDILKNLR
                     IYTYVIKELIK"
     gene            1712..2020
                     /locus_tag="PK768_08970"
     CDS             1712..2020
                     /locus_tag="PK768_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HQA61141.1"
                     /translation="MELEKLKDPYVDMLFDAILLLENREECYRFFEDICTIGEVQSMA
                     QRLQVAKMLKEGRTYTEIAQATGASTATISRVKRFLHYGANGYNTILERLGKRKQQED
                     "
     gene            2057..>2921
                     /locus_tag="PK768_08975"
     CDS             2057..>2921
                     /locus_tag="PK768_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQA61142.1"
                     /translation="MDFLQDLNSQQRKAVTHPAGPLLVLAGAGSGKTRVLTYRIAYLI
                     KNQAVNPRNIIAITFTNKAAQEMKERLQQLLPSVNDILVSTFHSACVRFLRFDIDKLG
                     YNRNFIIFDTQDSQALIKECLQALNIDEKKFAPAAVLNYISRAKDRLLEPSKCLDNAK
                     DIREKTMATLYELYQRRLKEHNALDFDDIIVKTVQLFKEFPAVLSYYQKRFLHILVDE
                     YQDTNLAQYELVRMMAAKHRNLCVVGDDDQSIYAFRGADIRNILEFEQDFPDATVIRL
                     EQNYRSTKTILN"
BASE COUNT          907 a          568 c          678 g          768 t
ORIGIN      
        1 ggtaggaata tcccgcattt acacgggtat gcactaccct tcggacgtat tgagcggtgc
       61 ctttttaggc atgctctgtg ccattgtaac acggcttgtc ataaattgat agtgccacag
      121 caggaggacg cttgaacagg gggacacttt gtatgacata caaagtgtcc ccctgttata
      181 accgctactc tcaaataact gctaaaatga ccataaaaaa aggatttatt gtactaaagt
      241 ggaatataat atttgcatcg gataatttat gctggaggtt tattaaatgg atattgaaca
      301 atatattact gcaaatgaga agaagctgat tgaggctacg agtgaaataa ttaaaataag
      361 gagcgtaaaa gaaagtccca tgcagggtat gccttttggt gaaggtcctg caaaggcact
      421 aaattatgca ctggatcttg cgaaaagctt cggccttgag acgaaaaacc ttgacaatta
      481 tgcaggatgg gctcaatggg gcgaaggcga tgaaatgata gggatacttg tgcatctcga
      541 tgtggttccc gaaggcagcg gctggactta tcctccctat ggcggtgaaa tacacgatgg
      601 cagaatttac ggcagagggg ccatagataa taaaggtcct gccatggctg cattgtttgc
      661 actgaaatct cttaaagatg ccggaattaa attcaacaga agggtccgag tgatatttgg
      721 caccgatgag gaaagtggct ttgagtgcat ggaatactac aaaaaacacg acgaagctcc
      781 tactatgggt ttttcgcctg atgcaaatta tcccatcata aatggcgaaa agggagtgct
      841 gactttttct tttaccactt tacttgacgc tgaaaaggta gaccagcgtg caagattgct
      901 gtcgtttaaa ggcggtcatc ggcctaatat ggtccccgat tatgccgaag cccacctgac
      961 cggtgatata gatgaaatca ctaatgcagt aaaaactgcc ttggatatcg taccgggaaa
     1021 ttttgaaata gaacaggcaa acgatgaaat tgtgatcaaa tcatacggtc tttctgccca
     1081 tggcagcacc cccgaaagag gcaaaaatgc ctgcatgctg ctggcaaagc ttttgggtga
     1141 agttaaactt gcaaagccct acgcagattt tatcggtttt ttgaatgaca agatagggct
     1201 cgacactacc ggcaaaggca tgggcatcga ttttaaagat gatatttccg ggcagcttac
     1261 ttttaatgtg ggtattgttg ggtgcgatga taataaagcc acggttacag ttaacattcg
     1321 ctaccccata aagtacaggg gtgatgagat aatactggaa atagagaaac acattcctga
     1381 ctctatccgc attgaaaata tatcagataa caaacctcac tatatccccg aagacagtat
     1441 aattatccaa aagcttaaag aagcttacga aaaggttacc ggtgaaaagg cctactgctt
     1501 ttctatcggc ggaggcactt atgctcgaat gtttgataac tgtgttgcat ttgggccagt
     1561 cttccccggt aagcccgagc tagcccatca gactgacgag tacattgaag tggaggatat
     1621 cctaaaaaat ttaaggatct acacttatgt aataaaagaa ttaatcaaat agcaactgag
     1681 ttcattagtt gctatacaag gaggggcagg tgtggaactg gaaaagttaa aggaccctta
     1741 cgtggatatg ctttttgatg ccattttgct tttggaaaat cgcgaagagt gctaccgttt
     1801 ctttgaagac atatgcacga ttggagaggt tcagtcaatg gcccagcggc tgcaggtggc
     1861 aaaaatgcta aaagaaggtc ggacatatac ggaaattgcc caggcaacag gtgcaagtac
     1921 ggccactatc agtcgggtta agcgtttttt gcactatggc gccaacggat acaatacgat
     1981 attagaaaga cttggaaaaa gaaagcaaca ggaagattaa taacgtcttc gctttttcgt
     2041 attaggaggt ttatcaatgg actttttaca ggatttaaat tcacagcaaa gaaaagcagt
     2101 aactcatccg gcaggcccgc ttctagtgct tgcaggggct ggcagcggta aaacccgtgt
     2161 cctcacctat cgcatcgcct atttgataaa gaatcaagct gtaaatccta ggaacataat
     2221 tgcaattact tttaccaata aagcggccca ggagatgaaa gagagactac aacaattgct
     2281 gccaagtgtc aacgacatac ttgtaagcac cttccactcg gcctgtgtaa gatttttgcg
     2341 ttttgatatc gataagttag gttacaatag gaattttata attttcgata cccaggattc
     2401 acaggctttg attaaagaat gcctgcaagc tctcaatatt gatgagaaga aatttgcacc
     2461 tgcggcagtt ttaaactata ttagccgtgc aaaagacagg cttttggaac cctccaagtg
     2521 tctcgacaat gccaaagaca tacgtgaaaa gaccatggca actttgtacg agttgtacca
     2581 aagacgactt aaagagcaca atgcattaga ttttgatgat atcatcgtga agacggttca
     2641 gctctttaag gaattcccgg cagtcctctc gtattaccaa aagcgctttt tgcatatctt
     2701 ggttgatgaa taccaggata ccaatttggc ccagtacgaa ttagtgagaa tgatggctgc
     2761 aaagcatcga aacttatgcg tagtgggcga tgatgaccag agcatatatg cttttagagg
     2821 tgccgatatt cgaaacatac ttgagtttga acaggacttt cccgatgcca cggttattcg
     2881 attggaacaa aattaccgct caacgaagac cattttaaat g
//
LOCUS       DAONUO010000270         2891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_23574_length_2891_cov_4.112130, whole genome shotgun sequence.
ACCESSION   DAONUO010000270 DAONUO010000000
VERSION     DAONUO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23574_length_2891_cov_4.112130"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1105)
                     /gene="rodA"
                     /locus_tag="PK768_08980"
     CDS             complement(8..1105)
                     /gene="rodA"
                     /locus_tag="PK768_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQA61143.1"
                     /translation="MNRKLLKNIEYPVLIAIVLLTILSVVIISSATHATSPEGSFRTA
                     RMQVMWFLAGLVAMFFVISIDYHSFAHWSNVIYVINILVLLLVLFSGEEGGGAQRWIG
                     IGSFRLQPSEFAKLALVITLSRHLEKKKSLNTLQDLLTVFLHMAVPMMLIARQPDLGT
                     ALVLVAMTFGMMFIAGISYKMLAGIISAGILSLPLFWQILKPYQRDRILVFFNPYLDP
                     LGKGYHVIQSKIAIGSGKLFGKGLYQGTQSQLDFLPVKHTDFIFAVLGEELGFVGGVI
                     LISLYFMLLYYSIRVAFKARDLLGTYMVVGVISMWAFQILVNIGMNVGIMPVTGIPLP
                     FMSYGGSSFIMNMMAAGLVLNVGMRRQKILF"
     gene            complement(1177..>2891)
                     /locus_tag="PK768_08985"
     CDS             complement(1177..>2891)
                     /locus_tag="PK768_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HQA61144.1"
                     /translation="MVQKQEKIYKVIDYEILEHESRVMYDGQIHDIEEVAKSVRTVKE
                     RLEKKLHIKLERAAVAAAGRALYTVKSEAEKMTSPFVEVTEADIRDLEADALSRAIKT
                     LHEHNKESGSGSTNYYCVGFSPIKWYLEDEALDNLIGQKGSRISVEIVATFLPRTVVE
                     SLMTVLSRCDLGLESLTLEPIAAGDIVVHPSMRKLNIALVDIGAGTSDIAISRDGSIF
                     AYGMVPMAGDEITEKICEEFLLSFDEAERVKRDLLVHEKVSFTDILGNQVELSTDEVL
                     LAIDSVIRELSAKIARKIVELNGKAPAAVMLIGGGSILPNLSDYIAGNLELPKERVGV
                     KGREGLKNIHGCEEFSGPFSVTPIGIAVNSLKGAHLSSYKVYIDEKPINILAQDNPTV
                     LDALLYSGKSSAEIFGRPGLAKTFHLNGKLVIVKGQMPQAATVVMDGRRVDLNTPVQD
                     GAVIKFNPAQDGKMASAKIKDYVTDSDKVFIKINGREFTVDPIAKISGQLASLEDEIP
                     DDAFVTIEPRDMILSDIFNLISFKPESMTGKLTMKINGQDASFTSAVNNNDEIDIYWE
                     NMHK"
BASE COUNT          829 a          684 c          531 g          847 t
ORIGIN      
        1 taccacacta aaaaagtatt ttttgtcgcc gcattcccac attgagtacg aggccagcag
       61 ccatcatgtt cattataaag gaactacctc cgtaactcat aaagggcagc ggtataccgg
      121 ttaccggcat aatgcccaca ttcatcccga tattgaccaa tatttgaaat gcccacatgg
      181 agataacgcc tacaaccatg taagttccca gcaaatctct ggccttaaaa gccactcgta
      241 ttgaataata gagcagcatg aaatataggc ttattaggat aactccacct acaaatccca
      301 attcttcacc taatactgca aaaataaagt ccgtatgctt taccggaaga aaatccaact
      361 gactttgagt tccctggtaa aggcccttac caaatagctt gccggaaccg atggcaattt
      421 tagactgtat tacgtgataa ccctttccca agggatccag gtatgggtta aaaaacacca
      481 gtattctatc tctctggtag ggctttaaaa tttgccagaa aagaggtaat gaaagaatgc
      541 ctgcagagat tatacctgca agcattttgt aacttatgcc ggcaataaac atcatgccaa
      601 aggtcatggc taccaatacc agggccgttc ccaaatccgg ctgtcttgca atgagcatca
      661 tcgggacagc catgtgcaga aaaactgtaa gcagatcctg cagggtattt aaagatttct
      721 ttttttccag atgcctggat aaggtaatta caagggcgag cttggcaaat tccgatggtt
      781 gcaatctaaa agagccaatg cctatccatc tctgggctcc gccgccctct tcacctgaga
      841 ataataccaa taagagcacc aaaatattta tcacatatat gacattagac caatgagcaa
      901 aagaatgata gtcaatagaa atgacaaaga acatagctac aagtcccgcc aaaaaccaca
      961 taacctgcat acgggccgta cggaagctgc cttcgggact tgtggcgtga gttgcactgc
     1021 tgataatcac aacacttaaa attgtaagta aaactattgc gattaataca ggatattcta
     1081 tatttttaag tagtttccga ttcatacctt atgcccccta aacccgtaaa cattatacca
     1141 taaatgacag gctccaatta gaacatttgg cgaaaattat ttatgcatgt tttcccaata
     1201 tatatctatt tcgtcattat tgtttacggc tgatgtaaaa gacgcatcct ggccatttat
     1261 tttcatggtc agttttcctg tcatgctttc gggtttaaag gatataagat tgaaaatatc
     1321 acttaaaatc atatctctag gctcaatagt aacaaaggca tcatccggga tttcatcttc
     1381 tagtgatgcc aactgtcccg atatttttgc gatggggtca acggtaaatt cccgtccgtt
     1441 tattttaata aataccttat cactatcagt aacataatcc ttaattttgg cagaagccat
     1501 tttgccgtcc tgtgcgggat tgaacttaat aactgcacca tcctgaacgg gcgtgttaag
     1561 gtcaactcgc ctgccatcca tgacaacggt tgccgcttgc ggcatttgac cttttactat
     1621 tactaacttt ccgtttaaat gaaaagtctt ggcaagcccg ggtctgccga agatttctgc
     1681 agatgatttc ccggaatata ggagggcatc taaaactgtg gggttgtcct gggctaatat
     1741 attgatcggt ttttcgtcta tgtaaacctt atatgaagac agatgggcac ctttaagcga
     1801 gtttacggct attccaatgg gagttaccga aaaaggtcct gaaaattctt cgcatccgtg
     1861 aatattctta aggccttcac gccctttgac accaactcgt tctttgggaa gttccagatt
     1921 ccccgctatg taatccgata agtttggcag aatgctgcca ccaccgatca acattactgc
     1981 agcaggagcc ttaccgttta gctcaacaat tttacgagca atttttgctg aaagctctct
     2041 tatcaccgaa tcgatggcta gcaacacttc atcagtcgat agttccacct gatttcccag
     2101 tatatccgta aaactaactt tttcatggac taaaagatcc cttttaaccc gctcagcttc
     2161 atcaaagctc agcaggaatt cttcacagat tttttccgtt atttcatcac ctgccatcgg
     2221 caccatgccg taggcaaaaa tacttccatc tcgggaaatg gctatatccg atgtgcccgc
     2281 tcctatgtca accaatgcta tattgagctt ccgcatactt ggatgaacca ctatgtcccc
     2341 tgccgcaatg ggctccagtg ttaaactttc aaggcctaaa tcgcatctgc taagcacagt
     2401 cattaggctt tcaacaacgg tgcggggaag aaatgtagca actatttcaa cggatattct
     2461 gctgcctttt tgtcctatta aattgtccaa agcctcatcc tctaaatacc attttatcgg
     2521 actaaaaccc acgcagtaat agttggtaga accagaccca gactccttgt tgtgttcgtg
     2581 caaagtttta atagctcttg acagggcatc agcttctaaa tcgcgaatat ccgcctctgt
     2641 gacctcaaca aagggagaag tcattttttc ggcttccgat tttactgtat aaagggccct
     2701 gccagccgcg gctaccgctg ctctctcaag cttgatgtga agtttttttt ccagcctttc
     2761 ttttactgtt cttacgcttt ttgccacttc ttctatgtca tggatctgac cgtcatacat
     2821 aactctactt tcatgttcta atatttcata atcgattacc ttgtaaatct tttcctgctt
     2881 ttgaaccata a
//
LOCUS       DAONUO010000271         2875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_23764_length_2875_cov_2.861348, whole genome shotgun sequence.
ACCESSION   DAONUO010000271 DAONUO010000000
VERSION     DAONUO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23764_length_2875_cov_2.861348"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PK768_08990"
     CDS             complement(<1..492)
                     /locus_tag="PK768_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HQA61145.1"
                     /translation="MTAQIHVFRVFDKNFVLDVNSGSVLQIDDLAFDVLKNYYLDSEE
                     KALQDLQSKYPKAKIEEVLDEIQILKEEGYLFTDMDLKPVLEKLENSYHVKALCLNVA
                     HDCNLRCKYCFASKGDYSGKRELMSPQIGKMAVDFLVNNSGNMRNLEIDFFGGEPLMA
                     LETV"
     gene            complement(651..1445)
                     /locus_tag="PK768_08995"
     CDS             complement(651..1445)
                     /locus_tag="PK768_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQA61146.1"
                     /translation="MSKRVFGLFLFSLFAVLIFGQAFANASEVFNLSKINQGIVGINI
                     PLKNNKTKIMIQKDGKTYYYDVKTEKDMLPLQMGSGIYLLALLENIEGSKYAVNSSKS
                     VQVNLDNEKISFLQSIRPVYWRKESKAAKLAKELTENMDTDEKKACAIYDYIINNIEY
                     DRNKLTNLNTDYIPNVDEIIASGKGICFDYAAVYAAMLRSIDIPAKLVMGYKEDIDAY
                     HSWNEVFLEKKGWVTIDTTYDAVMIEYGTSSTFEKDPGEYKKEKEY"
     regulatory      complement(1460..1536)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1665..2330)
                     /locus_tag="PK768_09000"
     CDS             complement(1665..2330)
                     /locus_tag="PK768_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HQA61147.1"
                     /translation="MRKFLGLILVILGILTISFPKIRDAYYDYQQKKLLDSLIQRWEE
                     LDDYNEIEQYPVDNVPTMDENIAEDDNEYLARYIEAHMEGILKIEKISLEMPILKGAT
                     RTNLNISAASLDGTGRPGQVGNYCISAHRGRSYGRLFNRLDELERGDLVEIIVKDTIY
                     TYEVLEKLLVEAKDNRILLPEENQKLLTLITCDYSRRPYPRLVVKAKLLDEKQQNTGK
                     NKE"
     gene            complement(2441..>2875)
                     /locus_tag="PK768_09005"
     CDS             complement(2441..>2875)
                     /locus_tag="PK768_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61148.1"
                     /translation="GYVLIGSIAPETTKTIYFSADFPEDAGNDYQGASLRVNWTFLTE
                     SVGGGGDDNGDDDDDDGDNGKKEPPIEVEEEPVPRGEPTIPDVPVPVPTEPDQPVVDI
                     PEEEVPSGPPDMPETGERVRYPFYIIGTFAILAGVSIIKKRL"
BASE COUNT          756 a          632 c          520 g          967 t
ORIGIN      
        1 taccgtttca agagccataa ggggttcccc gccaaaaaaa tcaatttcta ggtttctcat
       61 gttacccgaa ttatttacca aaaaatcaac ggccattttt cctatttggg ggctcatcaa
      121 ttctcgtttt ccgctgtaat cgccttttga agcaaagcag tatttgcagc gcaaattgca
      181 gtcatgggct acgttaaggc atagggcttt cacatgatag ctgttttcca gtttttctaa
      241 aaccggctta agatccatgt cggtaaaaag gtagccttct tcttttaaaa tttgaatttc
      301 gtcgaggact tcttctattt ttgctttggg atattttgat tgaagatcct gcaaagcctt
      361 ttcctcggaa tctaagtaat agttctttag gacatcaaaa gccaaatcat caatctgcaa
      421 taccgatccg ctgtttacgt ctagaacaaa attcttatca aatacacgaa atacatgaat
      481 ttgggctgtc aagtgtatcc tctcccatat tgcaacaatg tttgtgtagc agtattcatt
      541 ttaacatata tgttactttc attaaagatg gtctagaagc aaaaaaggtc ttgctgccgt
      601 ttttataatt taagatcagc aagaccctat atgtataaag cagggtatat ttagtattcc
      661 ttttcttttt tgtactcccc tgggtctttt tcaaatgtgc tgcttgtccc gtactcgatc
      721 atgaccgcat cataagtagt atcaatcgtt acccaacctt ttttttccaa gaacacttcg
      781 ttccatgaat ggtatgcatc gatatcttcc ttgtatccca tcaccagctt cgctggaatg
      841 tctatgcttc ggagcatagc agcgtacact gcggcatagt caaaacatat gcccttacct
      901 gatgctatga tttcatcgac gttaggtatg taatcggtat ttaaatttgt cagtttgttc
      961 ctgtcgtact ctatattatt tattatgtaa tcgtatatcg cacatgcctt tttctcgtca
     1021 gtatccatgt tctcggtcag ctcttttgcc agtttggcag ctttgctttc tttacgccag
     1081 tagacgggtc ttatagattg taaaaatgat atcttctcat tatcaagatt aacctgaacg
     1141 cttttactgc tattcaccgc atatttgcta ccttctatgt tttctaaaag tgccagtaga
     1201 tatataccgg atcccatttg gaggggaagc atatctttct cagtctttac atcgtaataa
     1261 taggtcttcc catccttttg tatcattatc tttgtcttgt tatttttcaa gggaatgttt
     1321 atccctacta ttccctgatt aatttttgac agattaaaca cttcagaagc attagcaaaa
     1381 gcttgtccaa atataagcac agcaaacaga gaaaacaaaa atagcccgaa gaccctcttt
     1441 gacaaaaata aactctcctt tcggcaactg gctgccttta aaaaaggccc tatagctttg
     1501 cgtccccgcg tttccacggg tttgccatta tcgcttgaaa gtttatttga cttaagcaac
     1561 ctgcattatt ctctaatttt tgaaagcaaa gcttgtataa attttaccat tttaatacat
     1621 gccaacaaag ctcaatcaat gcagattact aatatatttt tacattattc tttatttttc
     1681 ccagtatttt gttgtttttc gtcgagtagc tttgctttaa caacgagcct cggatagggt
     1741 cttcggctat aatcacaggt aatcagggtc agaagttttt gattttcttc ggggagaagt
     1801 atccggttgt ctttagcttc taccagaagt ttttctagta cctcataggt atatattgta
     1861 tctttcacta taatctctac caaatcacct ctttccagtt catccaagcg attgaaaagt
     1921 ctgccgtatg agcggcccct gtgagcggaa atgcagtaat tgcctacctg tcccggcctt
     1981 ccggtgccat ctaaactggc ggcagaaatg tttagattgg tccttgtggc tcccttaagg
     2041 ataggcattt caagggatat tttttctatc tttaatatgc cttccatgtg ggcttcgata
     2101 tatctggcca ggtattcatt atcatcttca gcaatgtttt catccattgt gggaacattg
     2161 tctaccggat attgttcaat ttcattgtag tcatcgagct cttcccatct ttggattaaa
     2221 gaatcaagga gttttttctg ctggtagtca taatatgcat ctctgatttt tggaaaggaa
     2281 atagtaagga ttccaaggat tacaaggatt aagcctagga attttctcaa tctcaccgcc
     2341 acctaaaaaa atcgcccgga ttatacttgc tgtatactcc aggcgaataa taggagtaaa
     2401 agaattctat tggtctacta ttgtaaacac ttaaaacagc ttacaatctc ttcttaataa
     2461 tactaacccc tgccaaaata gcgaaggtac ctataatgta aaacgggtag cgcacacgct
     2521 ctcctgtctc cggcatatca ggcggccctg aggggacttc ctcttcgggg atatctacga
     2581 caggctgatc cggctctgtt ggaaccggaa cagggacatc gggaatagtt ggttcccctc
     2641 taggaacagg ttcctcttcc acttcaatcg gcggctcttt tttgccattg tcgccatcat
     2701 cgtcatcatc gtcgccgtta tcgtcccctc ctccaccgac gctctcggta agaaaagtcc
     2761 agtttactct aagtgaagca ccctgataat cgtttccggc gtcctcagga aaatccgcgg
     2821 aaaagtagat ggttttggta gtttccgggg ctatgcttcc tattaataca tatcc
//
LOCUS       DAONUO010000272         2847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_24033_length_2847_cov_4.031877, whole genome shotgun sequence.
ACCESSION   DAONUO010000272 DAONUO010000000
VERSION     DAONUO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24033_length_2847_cov_4.031877"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PK768_09010"
     CDS             <1..1609
                     /locus_tag="PK768_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HQA61149.1"
                     /translation="FIGEGVNSLPVEFRNGIDVMTTETGCWSSIWETDDLVRDYFETH
                     GRPGDFDRISPDEITLYDGLVEVDLSKIKPSIALPFHPSNVYTIEELKENAEDIFTDI
                     EKNSKETFSSNEIKIDLISKIRNGQIRFEQGIIVGCSGGTFDNIMAAADILRGKSIGN
                     GSFSLSIYPGSQPTYLELVRNGAVADLMAAGVLVKPAFCGPCFGAGDTPANNELSIRH
                     ATRNFPNREGSKPTEGQVSLVALMDARSIAATAVNEGILTAADEIDVNYSGKRYYFDK
                     TVYEKRVYNGWSKEDPSAELVYGPNIKDWPSMPSLTEHVLIKVVTYINDPVTTTDEII
                     PSGETSSYRSNPMKLAEFTFSRKDPAYVGKAKEVLKYELSREEGNNPEEVEELKEVYN
                     SIRKIEGFEDTNAQDIGIGSTIYANKPGDGSAREYAASCQRILGAWANIAKEYATKRY
                     RSNLINWGIIPFLIEDKSLIKKDDFIFIPNIKTAILNKTKDIKAYVLGDKIKEISLSI
                     GHLTDKETEILVSGSLINYNKKNKGNP"
     gene            1642..>2847
                     /locus_tag="PK768_09015"
     CDS             1642..>2847
                     /locus_tag="PK768_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQA61150.1"
                     /translation="MGLTITEKILSRALVSGNLVKGSRIGIKADQTLTHDLNGIMSYL
                     VLEAIGLDSKKVELAVQYIDHNMIQADHKNADDHNFILDMTSKLGIITARSGSGICHA
                     LHLEHFAAPGKVLIGGDSHTVAAGGIGSFAVGVGGFDNAMAIAGQPFYFTMPKIVRVN
                     LTGRLQPFVSAKNIILELLRIVGVKGGVGKIFEYGGDGVKTLSISERSTIANMGAETG
                     ATTSVFPSDENTRLWLKAFDREKDYIPLDADPDAEYDDVIEIHLDELEPLISLPSQPD
                     NVVKVKEVEGVKVDQVMIGSCTNASLQDILSIANILDGEIVSKDVELGVYPSTKTVLR
                     ESFRRGALDKLLEAGARIFEPICGGCNGCGFAPPTDGVSLRTTPRNFKGRSGTKTDKV
                     YLCAPETAAA"
BASE COUNT          906 a          579 c          674 g          688 t
ORIGIN      
        1 attcataggt gaaggtgtaa atagccttcc ggttgaattc agaaatggaa tcgatgtaat
       61 gacgacagag acaggttgct ggagctccat atgggaaacc gatgacctgg taagagatta
      121 cttcgaaaca cacggacgac caggcgactt tgacagaata agtcccgatg aaataacact
      181 atatgacggc cttgtagaag tagacctttc taaaattaaa ccttcaatcg cccttccctt
      241 ccatccgagt aacgtctata cgatagaaga attaaaggaa aatgctgagg atatattcac
      301 cgacattgaa aaaaatagca aggagacttt cagttccaat gaaattaaaa tagatttaat
      361 tagcaaaatt agaaacggcc aaattcgctt tgagcagggc ataatagttg gttgctcggg
      421 cggaaccttc gacaacatta tggccgctgc cgacatctta agggggaaat cgataggaaa
      481 tggaagtttt tctttgagca tatatcccgg aagtcagccc acttacctgg agctagttag
      541 aaatggtgcg gtggctgatt tgatggcagc cggggtttta gtcaagccgg cattttgcgg
      601 gccatgtttc ggcgccggag atactccagc caataatgag ctttccatta ggcatgcaac
      661 cagaaacttc cccaatagag aaggttccaa gccgacggaa ggccaggttt cattggtcgc
      721 acttatggat gccagatcta tagctgctac agctgtaaac gaaggcatct taactgccgc
      781 tgatgaaatt gatgtcaact actctggcaa gaggtactat tttgataaaa ccgtatacga
      841 aaagagggta tacaatggtt ggagcaagga agatccgtca gccgaattgg tttacggccc
      901 gaatatcaag gattggccca gtatgccgtc attgacggaa catgtcctca ttaaggttgt
      961 gacttatata aatgacccgg ttacaaccac cgatgaaatc ataccatcgg gtgaaacctc
     1021 ctcctatagg tctaacccca tgaaactggc agaatttact ttcagcagaa aggatccagc
     1081 atatgtgggc aaagctaagg aagtcctaaa atacgaatta tcaagggaag aaggaaacaa
     1141 tcctgaagaa gtagaggaat taaaagaagt atataattcc attcggaaaa tagaaggctt
     1201 tgaagatact aatgctcagg acataggaat cggcagcacc atttacgcaa ataaaccggg
     1261 agatggctct gcaagggaat atgctgcatc ctgtcagcgc atcctgggag cctgggcaaa
     1321 tatagcaaag gagtatgcaa caaaaaggta cagatcgaac ctcattaatt ggggaatcat
     1381 acccttcctg atagaagaca aatccctgat taaaaaagat gatttcatat ttatccccaa
     1441 catcaaaacc gccatattaa ataaaacaaa agatatcaag gcttatgtgc tgggagataa
     1501 aataaaggag atttccctgt caataggtca tttgactgat aaagagactg agattttagt
     1561 cagcggttca ttgataaatt acaacaaaaa gaataaagga aatccataat attccttaaa
     1621 tttaatgcgg agggatttct attgggtctg acaattaccg aaaagatact aagccgcgct
     1681 ttagttagcg ggaatcttgt aaaaggaagc aggatcggaa ttaaagcaga ccaaactttg
     1741 acccatgact taaacggcat tatgtcctat ctagttcttg aagccatcgg cctagacagt
     1801 aaaaaggttg agctggcagt tcaatatata gatcacaaca tgatacaggc cgaccataaa
     1861 aatgctgatg accacaattt tattttagat atgacatcaa agctgggaat aattactgcg
     1921 aggagcggca gcggcatatg ccatgccctc cacctcgaac actttgccgc accaggcaag
     1981 gtgctcattg gaggagattc acataccgta gctgcaggcg gtattggctc ctttgcggtg
     2041 ggtgtcggcg gctttgataa cgccatggcc attgccggcc agccctttta ttttacaatg
     2101 cccaaaatcg tccgggtcaa tttgacggga aggcttcagc ccttcgtttc ggctaagaat
     2161 ataattttag aattattgag aatcgttggt gtcaagggtg gtgtaggcaa gatttttgaa
     2221 tacggcggag atggtgtgaa aacccttagc atctcggaac gctcaactat tgcaaacatg
     2281 ggagcggaga cgggtgcaac tacctctgtc ttccccagtg atgaaaacac gagactctgg
     2341 cttaaagcat tcgatagaga aaaagactat atcccgctag atgcggatcc tgatgccgaa
     2401 tacgatgatg tcatagaaat acatttagac gaattggagc ccctaatatc attaccttct
     2461 cagcctgaca atgtggtaaa agtgaaagaa gttgaaggag taaaggtaga ccaggtgatg
     2521 atcggcagtt gtacaaatgc gtccttacag gatattctaa gcatcgcaaa tatattagac
     2581 ggggaaatag tatcaaaaga tgttgaactg ggtgtgtatc cctctacaaa gacagtgctt
     2641 cgggaaagct tcaggcgcgg tgccttggac aaactcttag aagccggcgc caggatattt
     2701 gagccgatct gcggaggctg caacggatgc ggctttgcac ctcctaccga tggcgtatct
     2761 ctaagaacta cgcctagaaa tttcaaaggc aggtcaggaa caaaaaccga caaggtctat
     2821 ctgtgcgctc cggaaaccgc tgcagct
//
LOCUS       DAONUO010000273         2841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_24095_length_2841_cov_3.823044, whole genome shotgun sequence.
ACCESSION   DAONUO010000273 DAONUO010000000
VERSION     DAONUO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24095_length_2841_cov_3.823044"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PK768_09020"
     CDS             <1..184
                     /locus_tag="PK768_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQA61151.1"
                     /translation="ATVIVRGEVLEKHPELEEVLNKLAGRIDDTKMAELNAAVDLDKR
                     EPKDVARQFLEEEGLI"
     gene            complement(210..1658)
                     /gene="guaB"
                     /locus_tag="PK768_09025"
     CDS             complement(210..1658)
                     /gene="guaB"
                     /locus_tag="PK768_09025"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQA61152.1"
                     /translation="MDKFSKEGLSFDDVLVIPAKSEVLPKDVDITTRLTNKIKLNIPI
                     ISAGMDTVTEARLAIAIAREGGIGIIHKNMSIERQALEVDKVKRSEHGVIVDPFYLSP
                     DDLISDALELMGRYKISGVPITEGTKLVGILTNRDLRFVDDTSRKISEVMTKDDLVTA
                     PEGTTLDEAKEILKKHKLEKLPIVDDNFNLKGLITIKDIEKAIKYPNSAKDEGGRLLV
                     GAAVGVTRDMEERLKALVEADVDVVVVDTAHGHSKGVIEAVYRIKELYPQLQVIAGNV
                     ATAEATRDLIKAGADAVKVGMGPGSICTTRVVAGIGVPQVTAIYDCSQEAQKYNVPII
                     ADGGIKYSGDIVKAIAAGADTVMLGSLFAGTEESPGEIEIYMGRSFKVYRGMGSVGAM
                     EEGSKDRYFQEDVKKFVPEGVEGRIPYKGPLSETVYQLIGGLRAGMGYCGARNIEELK
                     QAKFVKISSAGLRESHPHDIYITKEAPNYSQI"
     gene            2087..2398
                     /locus_tag="PK768_09030"
     CDS             2087..2398
                     /locus_tag="PK768_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HQA61153.1"
                     /translation="MFLLDLKLFLMAFCMLFCAELGDKTQLAVFTLVTQYKKPLPIFL
                     GASLALVFVTLIGALFGEVVSRYIPEAYVKLIAGILFVGIGIFVLIEALPEAIQQMGR
                     G"
     gene            2579..>2841
                     /locus_tag="PK768_09035"
     CDS             2579..>2841
                     /locus_tag="PK768_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61154.1"
                     /translation="MLSFGSLMITFSLVLMGSITWLGYLFSKRKMQDSDSYFTAGRDI
                     STAFMTATFIAYAVGTGLIFSPGEMAYLEGLTAMIGYGLAISLS"
BASE COUNT          694 a          659 c          615 g          873 t
ORIGIN      
        1 tgcaacggtt atagtgcggg gagaggtcct ggagaaacat ccggaattgg aggaagtgct
       61 caacaagttg gccggccgca ttgatgacac aaaaatggca gagctcaatg cagcggttga
      121 cctggacaag cgggaaccaa aagatgtggc ccgacaattt ttagaagagg aaggcttgat
      181 ataaaaaatt aggactggct tagccagtcc tatatttgtg agtaatttgg cgcttccttg
      241 gtgatatata tgtcatgggg atgactttcc cttaagcccg cagagctgat cttaacaaat
      301 tttgcctgct taagttcttc tatatttctg gctccgcagt agcccatgcc tgctctaagg
      361 ccgccaatga gctgatatac ggtctctgaa aggggtccct tataaggaat gcggccctct
      421 acgccttccg gtacaaactt tttcacatct tcctggaaat atcgatcttt gctgccttct
      481 tccatggccc caacggagcc catgccccgg tataccttaa aactgcggcc catgtatatc
      541 tctatttctc ccgggctttc ctcggtgcct gcaaaaaggc tcccaagcat taccgtatcg
      601 gccccggccg ctatggcctt cactatatct cccgaatact tgataccccc atcggcaata
      661 atgggtacat tgtatttttg tgcttcctgt gagcagtcat atattgccgt tacctgcggg
      721 actcctatgc cggccaccac cctcgtagta catattgaac cgggtcccat gcctaccttt
      781 accgcatcgg caccggcctt aattaaatca cgggtagcct ctgccgtggc cacattgccg
      841 gcaatgacct gcagttgtgg atagagttcc ttaatccgat aaaccgcctc aataacgcct
      901 ttcgaatgac cgtgtgcggt atctacgaca accacgtcta catcggcctc taccaaagcc
      961 tttaagcgct cctccatgtc ccttgtaact ccaacggccg cacctaccag cagcctgccg
     1021 ccttcgtcct ttgccgaatt ggggtacttg atggcctttt caatatcctt tatggtgatt
     1081 aaacccttta gattaaagtt gtcatcaact atgggcagct tctcaagctt gtgttttttg
     1141 aggatttcct tggcttcatc aagggtggtg ccctcagggg ccgtcaccaa atcgtccttg
     1201 gtcatgacct cgctgatttt tctactggta tcatctacaa aacgaaggtc cctgttagtc
     1261 agaatgccga ccaattttgt tccctcggta atgggcacac cggatatttt gtagcgtccc
     1321 atcagctcta aggcatcgct aattagatca tccggcgata ggtagaaggg gtcaactatt
     1381 accccgtgct ccgaacgctt gaccttgtcc acttccaggg cttgcctttc aatggacatg
     1441 ttcttgtgga taatgccaat accgccttcc ctggcaatgg caattgccag ccgagcctcc
     1501 gttaccgtat ccattcctgc gctgataata ggtatgttca gtttgatttt attggtaagt
     1561 cgggtagtaa tatccacatc tttcggcaat acctcggatt ttgcaggtat tactaaaaca
     1621 tcatcgaaac ttaaaccctc cttgctgaat ttatccaaaa aatcgccccc aaataacatt
     1681 ctccgatatt ttattattcc aaagtttatc aaagtttaat atcggtgtca ataataatca
     1741 aacagggcaa cggcgcttta ttttgagccg gttacttttg tgctgcaggt attcggctat
     1801 attgcatagg catggtcaag tgcttatttg tcaggcagtt ctttaaatat ttgctggaca
     1861 ggccttgata aatatgtagc aatgttggta taatagaatc atgctgaata taatatccgc
     1921 ataacggagg caataaaggg gagtagttcg ggccttggcc tttgaaagtt caacatgctg
     1981 gcaaaacatt tgcctggact ttcaccgctt atgggaatga gacctttatt ccaatacttg
     2041 attattggaa taaaggtctt tgttgtaatt aataatttat aatgaggtgt ttcttttgga
     2101 cttaaagctc tttttaatgg cattttgtat gttgttttgt gcggaactcg gagataagac
     2161 ccaactggct gttttcacgc tggtaacgca gtacaaaaag ccccttccca tattcctggg
     2221 ggcttccctt gcactggtgt ttgttacact gatcggcgcc ttgttcggag aggtggtatc
     2281 acgatatatc ccagaagcat atgttaaact tattgctggg attttatttg tagggatagg
     2341 gatcttcgtt ctaattgagg cccttccaga agccattcag cagatgggcc gcggatagac
     2401 atgtgagatt gtagcgacaa ttttcgtcac tacaatctct ttttttaatt tcacgtgtaa
     2461 acatttttgc acattgacaa atgccctttt taatgcttta attaaatacg gacattttat
     2521 tattttttca tggtaatgtt cggtattatt cgacttgcta caggagggat tatttataat
     2581 gctcagcttt ggatctctaa tgattacttt tagcctggta ctaatgggct caattacttg
     2641 gctaggttac ctatttagta agaggaaaat gcaagacagc gactcgtatt ttactgcagg
     2701 cagggatatc tctacagcat ttatgactgc aactttcata gcttatgctg taggcaccgg
     2761 ccttattttc tctccaggtg aaatggcata tttggaagga cttactgcca tgataggata
     2821 tggactggct atctctttat c
//
LOCUS       DAONUO010000274         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_24295_length_2823_cov_3.131503, whole genome shotgun sequence.
ACCESSION   DAONUO010000274 DAONUO010000000
VERSION     DAONUO010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24295_length_2823_cov_3.131503"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK768_09040"
     CDS             <1..216
                     /locus_tag="PK768_09040"
                     /inference="COORDINATES: protein motif:HMM:NF025626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HQA61155.1"
                     /translation="QCPFTAKYVPLIEGIAHSRNTPFKSIRFETTEQAQNAPAPFTTY
                     SLFYNGEFITNEILSDKKFEKMLINLL"
     gene            376..1182
                     /locus_tag="PK768_09045"
     CDS             376..1182
                     /locus_tag="PK768_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA61156.1"
                     /translation="MCEFCTKHGEGKKWYEIMENYSRELLNDPGRRTYIEQFVPRVRK
                     SSKDNLSRLEWVKKKMPLAHRFIRKIANVSAKKFHFGQVVPLEDAEKIVEMVQTITRV
                     PCVCRTVLAGKSNARYCLLLGINPTDYNVDWPELKANLEVLTPSEAKMVLRDFDKKGL
                     VHSIWTLKSPFIGAICNCDRDCLAYRYQVGSDLLELMFKAEYRAKIDMELCIGCRNCM
                     SLCQFGAIEYSVTYGKCYINTEKCYGCGVCRVACKTQAIKLYDIEEVFVV"
     gene            1700..>2823
                     /locus_tag="PK768_09050"
     CDS             1700..>2823
                     /locus_tag="PK768_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA61157.1"
                     /translation="MNGKKIKYVASLIILVTMLVGIIVPSGIAAPSTVLNDISDHWAK
                     DKIRQMVNKGIVSGYGDGTFKPDNNITRAEFMALVNRSFGFNKKAATNFTDVQRTDWF
                     YDEVVKAIAAGYISGYPDGTVKPNKEISRQEAAVALCKALNLSVKPQALNFTDRDSIP
                     PWSKDYIGTLAEKAYMTGYPDGSFRPDRFITRAETITMLDKALAGEKGTYYLPGVYGP
                     EEGIETVKQDIVINLPGITLRNMIIEGDLYLNEGIAEGDITLENVTVKGKTIINGGAN
                     IDLIDFSCKEVEVNKGARNARITASGDTKIKRLNLNCGVHLKADGIDELRILNDDKVT
                     LEGNFENVNVEGNGNIEVLKNTTINNLRINEESDVELKEGS"
BASE COUNT          948 a          524 c          640 g          711 t
ORIGIN      
        1 caatgcccat ttaccgccaa atacgttccc ctgattgaag gcattgctca ttcaagaaat
       61 acacctttta aatcaattcg atttgagact acagaacagg ctcaaaatgc gccggctcct
      121 tttactacat acagtctgtt ttataatggt gagtttataa caaacgaaat actatcagat
      181 aaaaaatttg aaaaaatgct aatcaacttg ctataagcag ctatacaccg aaaaaagctt
      241 gaaatgctgt gcgataaggc acagcttttt taattgtaaa tgctcgcctt ttgcataaaa
      301 tgtgaattaa ataccaatta agtggaaaaa atcatatata tccatccata tatttaatat
      361 tttggaggtg gtagaatgtg tgaattctgc accaagcacg gtgaagggaa aaagtggtac
      421 gaaatcatgg agaattactc aagggaactg ttgaatgacc cgggccgcag gacttacata
      481 gagcagttcg ttcccagagt gcgcaaaagt tccaaagaca atttatcgag actggaatgg
      541 gtaaaaaaga agatgcccct tgcccaccgc tttataagaa agatagccaa tgtcagtgcc
      601 aaaaagttcc acttcggtca ggtggttccc cttgaagatg ctgaaaaaat agtagaaatg
      661 gttcagacca tcacgagagt gccctgtgtg tgccgcaccg tacttgccgg aaaaagcaat
      721 gccaggtact gcctgctcct tggaataaac cctacggact acaatgttga ttggccggaa
      781 ctaaaggcaa atctggaagt gctgacccct tcggaagcaa aaatggttct gagggatttt
      841 gacaaaaaag gcctggttca ttctatttgg acccttaaga gcccttttat aggagctatt
      901 tgcaactgcg atcgggactg ccttgcttac aggtaccagg taggttcgga cctgcttgag
      961 ctcatgttca aggctgagta tagggctaag atagatatgg aactttgcat tggatgccgg
     1021 aactgtatga gcctttgtca attcggtgcg atagaatatt ccgttaccta tggcaaatgc
     1081 tatataaaca ccgaaaaatg ttacggttgc ggcgtatgta gggttgcctg taaaacacaa
     1141 gcgattaagc tttacgacat agaagaagtt tttgtcgtat agaaggagct aatagggccc
     1201 gcaaaagaaa ttttcatcaa gagtttttat ggtatttcag attttcccct attctaccat
     1261 aatttatgtc cgaggaaatc aaaaattatc gaaaaataca gacaaattat tgcccttcga
     1321 aggaggaata tttttctaaa aaaagaataa aataaactac attacacagt tgaattatcc
     1381 ataatattat ggcgctggag gtagaaggac aaatttgttc aactgcatct taccggcaaa
     1441 tcatctcagg cgattgctga gagagtatcg ccgttaattt tttagtccca aaatgcgtaa
     1501 ctagcagctt tagaattcag aaaataaaaa atatagagaa cagggaggtg attaaaaaca
     1561 tgtagatcac aaggaaaggt ggtgtgagtt ctaagataac cctgcaggtc aggccaatat
     1621 gatggggcta acaggggagt attgtcgcaa cttgaatcgg ctactacctt attagctaaa
     1681 attgttggag gtgacaatat tgaatggaaa aaaaattaag tatgtagcaa gtttaataat
     1741 acttgttacg atgctcgtgg gaatcatagt tccttcaggg atagcagctc caagcacggt
     1801 acttaacgac ataagtgacc actgggccaa agataaaata aggcaaatgg tcaataaggg
     1861 cattgtcagc ggatatggag atgggacatt taaaccagat aacaatataa ccagggctga
     1921 attcatggcc cttgtcaaca ggtcctttgg ctttaacaaa aaggctgcaa caaattttac
     1981 agatgttcaa agaacggatt ggttttacga tgaagtggtc aaagcaatag ctgcagggta
     2041 catttcaggt tatccggatg gaactgtaaa gcccaacaag gaaataagcc gtcaagaggc
     2101 tgccgtggca ctttgtaagg cgctaaatct ttccgtgaaa cctcaggcgc taaactttac
     2161 cgatagagac agcattcccc catggagtaa ggactatatc ggcaccttgg ccgagaaagc
     2221 ttacatgacg ggctatcccg acggaagctt cagaccggac cgcttcataa cccgggcgga
     2281 aaccataacc atgttggaca aagctctggc aggagagaag ggcacgtatt atttaccggg
     2341 cgtttacggc cccgaagaag gcatcgaaac cgttaagcag gatattgtaa ttaacctgcc
     2401 gggcataacg cttcgcaaca tgattattga gggagacctc tatttgaatg aaggtatagc
     2461 tgaaggagat ataaccttag aaaatgtaac ggttaaaggc aaaacgatca tcaatggtgg
     2521 ggcaaacatt gaccttattg actttagctg taaagaagta gaggtaaaca aaggggccag
     2581 aaatgccaga atcactgcat caggtgacac aaaaatcaag agattaaact tgaattgtgg
     2641 tgtgcactta aaagctgacg gaatcgatga actaagaatt ttaaatgatg acaaggtaac
     2701 ccttgaaggc aattttgaga atgtcaacgt ggaaggcaat ggcaacatag aagtgttgaa
     2761 aaataccacc atcaataatc tccggataaa tgaagaatcc gatgtagaac taaaagaagg
     2821 ttc
//
LOCUS       DAONUO010000275         2795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_24582_length_2795_cov_3.616788, whole genome shotgun sequence.
ACCESSION   DAONUO010000275 DAONUO010000000
VERSION     DAONUO010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24582_length_2795_cov_3.616788"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..436)
                     /locus_tag="PK768_09055"
     CDS             complement(173..436)
                     /locus_tag="PK768_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA61158.1"
                     /translation="MAYFKHLYNDDQLPNRAKLVYVYLHDRMDNEKKAWPGINRIASD
                     LSLSRSTVKRAIGDLVKSGYIRKEVAYREKNRGQTSNRYYLEK"
     gene            complement(451..2292)
                     /locus_tag="PK768_09060"
     CDS             complement(451..2292)
                     /locus_tag="PK768_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HQA61159.1"
                     /translation="MSNIGLLVLIGVVMFGGLSLIPLLAHIYNLNGIKSKTVGDGQHG
                     TARFASRSELRRTYKQIPFEPELWRKGINLPKAQGIVVGCKEKGRHTVALVDEGDVHA
                     MMIGAAGCGKTAFFLYPNLEYACACGMSFLSTDTKGDLVRNYGTIAKKYYGYNVSLID
                     LRNPTRSDGNNLLHLVNKYMDEYLKATDNIAAKAKAEKYAKITAKTIVSADGNNADYG
                     QNAFFYDAAEGLLTAAILLIAEFAPKKQRHIVSVFKLIQDLLAPSGIKGKNQFQLLME
                     RLPSEHKAKWFAGAALNTGDQAMQSVMSTALSRLNSFLDSELEQILCFDTAIDAEKFC
                     NEKSAVFVVMPEEDPNKFFMISLIIQQLYREILSVADANGGKLKNRVMFYCDEFGTLP
                     PISSAEMMYSASRSRRLSIISIVQSHQQLEKNYGREGAAIIIDNCQLTIAGGFAPGSE
                     SAEIISKAMGSRTVMSGSVSRSKNAPSQSLQMIERPLMTPSELKSMKKGRFVVLKTGT
                     NPFIARLKLFFKWGIKFDEKNPYTVSDKGARSVSYASKTQIEDAIITKFPPPSSMKST
                     YLSKEYHDMSLHPKDVDRKGDDSGTGTEMTPKNRIRSVGSGQALKTE"
     gene            complement(2366..>2795)
                     /locus_tag="PK768_09065"
     CDS             complement(2366..>2795)
                     /locus_tag="PK768_09065"
                     /inference="COORDINATES: protein motif:HMM:NF018601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HQA61160.1"
                     /translation="ELIPTIVTSLESVIETLIKSGIIYFRCGGAIGFDLLAGYIVLKM
                     KKRYPKIRLIMVLPCENQDLKWSPADKKRYRQLLVSADETIYIQSAYDNQCMHKRNRH
                     LVDNSGVCIAYLTNDRSGTGYTVRYAKSKGKEIINIAKMI"
BASE COUNT          751 a          633 c          547 g          864 t
ORIGIN      
        1 gaatagattg ttttaacatc tgcaaaatta acgaatacat tatatctttg ttttttctct
       61 ggtagtaata actcttatag tgggtacttc aagggagttc tttatgaacc tcccccgagt
      121 tcaagagcat gacgcaataa ccccttggcg gagaattctg gtttttacac tgctattttt
      181 ctaaataata gcggtttgag gtttgccccc ggttcttttc ccggtaggcc acttctttcc
      241 gaatatatcc ggatttgacc aaatcgccta tagcacgttt taccgtactg cgtgagagcg
      301 atagatcgga cgcaatacga ttgattccgg gccaagcttt tttttcattg tccattcggt
      361 catgcaaata tacatagacc agcttcgccc tattaggaag ctggtcatca ttataaagat
      421 gtttgaaata cgccataggc acatcatcct ttattctgtt tttaatgctt gaccgctacc
      481 aacactacgt atgcggttct tcggtgtcat ttcagttccg gtgccactat catctccctt
      541 cctatccaca tcctttggat gtagcgacat atcgtgatat tctttagaca aataagtcga
      601 tttcatgctg cttggtggtg ggaactttgt gataatggca tcttctatct gcgtcttgga
      661 tgcataggat accgagcgag cgcctttatc cgaaacagtg taagggttct tttcatcaaa
      721 cttaatgccc catttgaaga acaactttaa tctcgctata aatggatttg tgccggtttt
      781 taaaacaaca aaccggcctt ttttcatgct ctttagttcg gaaggagtca taagagggcg
      841 ttcaatcatt tgcagggact gggagggagc attcttactt cgactgacag aaccggacat
      901 aaccgttcga cttcccattg ccttactaat gatttccgca ctctcactac ccggcgcaaa
      961 gccacctgca atagttagct gacagttatc gataataatg gctgcccctt cgcgaccata
     1021 gttcttttca agctgttgat gggactgaac gatgctaata atagagagcc gcctacttcg
     1081 agaggcactg tacatcatct ccgctgaact aatagggggt agcgttccaa actcatcgca
     1141 gtaaaacatt acacgattct ttaatttacc gccattggca tccgctaccg acagaatttc
     1201 ccgatatagt tgctgaataa tcagactaat cataaagaac ttattaggat cttcctccgg
     1261 cataacaaca aacacagcgg atttttcgtt acaaaacttt tctgcatcga tagcagtatc
     1321 aaaacacaat atttgttcta gctctgaatc gaggaatgag tttagccgag agagtgcggt
     1381 agacataacc gattgcatcg cctgatcccc ggtattcaaa gcggctccgg caaaccattt
     1441 tgctttatgc tcacttggta gcctttccat taggagctgg aactgatttt tacccttgat
     1501 tcctgaagga gcgagtaaat cctgaatcag cttaaacacc gacacaatat gtcgctgttt
     1561 ctttggagca aactcagcaa tcagcaaaat ggcagctgtc aatagtcctt cagcagcatc
     1621 atagaaaaat gcgttttgcc cgtaatctgc attattgccg tctgcactga caatggtctt
     1681 ggcagtaatt ttagcgtatt tttctgcttt tgctttagcg gcaatattat ccgttgcctt
     1741 tagatattca tccatatatt tgttaacgag atgcagtaga ttattgccat cggaccgagt
     1801 aggattgcga agatcaatca gtgacacgtt gtaaccgtaa tactttttag caatggttcc
     1861 gtaatttcgg acaagatctc ccttggtgtc ggttgaaaga aaagacattc cacaagcaca
     1921 ggcatattct aagtttggat ataaaaagaa agctgtttta ccacagcctg ctgcaccgat
     1981 catcatggca tggacatcgc cctcgtccac caatgcaacg gtatggcgtc ctttttcttt
     2041 gcatccaaca acaattccct gcgccttggg aaggttaatt cctttccgcc ataattccgg
     2101 ctcaaaagga atctgcttgt aagtgcgtcg caactccgac cgtgacgcaa accgtgcggt
     2161 tccgtgctga ccgtcgccta ccgttttaga cttgattccg ttaaggttgt atatgtgggc
     2221 taatagcgga atcagactca gcccaccaaa cataacaaca ccgataagaa ctaaaagacc
     2281 aatattactc atgaaaatcg ccccttacat aatttttggt atgtaagggg cttttttatg
     2341 tgagctatat agcgaatata gcaacctata tcatttttgc aatgtttatg atctctttac
     2401 ctttcgattt agcgtatcga acagtgtatc cggtaccgct tcgatcgttt gttaaatagg
     2461 ctatacatac tccgctgtta tccactaggt gacggtttct tttatgcatg cattgattat
     2521 cataagcgga ttgtatatat atcgtttcat cagctgacac taacaattgc ctgtaccttt
     2581 tcttatctgc cgggctccat tttaaatctt gattttcaca gggcaaaacc ataatcaaac
     2641 ggatttttgg gtaacgtttt ttcatcttaa ggacgatata tccagcaaga agatcgaatc
     2701 cgatcgcacc gccacatcga aaataaatga ttccactttt tattaaggtt tctatcaccg
     2761 attccagaga ggttacaatt gtaggtatta gttca
//
LOCUS       DAONUO010000276         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_24705_length_2785_cov_3.546154, whole genome shotgun sequence.
ACCESSION   DAONUO010000276 DAONUO010000000
VERSION     DAONUO010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24705_length_2785_cov_3.546154"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="PK768_09070"
     CDS             <1..1196
                     /locus_tag="PK768_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQA61161.1"
                     /translation="ISQIGKIKTEFEFQKIINARNMLAMPGLINAHTHLAMVLFRNYA
                     DDMPLFDWLTKKIWPLEANLTPEAIYSGSILGIAELIKSGVTGFLDMYFFADETIRAA
                     LETGVRVYIARGLTDEEEGKEVQLEETRRLFNEYHEWDGKVKIFAGPHAPYTCSPRYL
                     KKVVELAQELDIGIHIHLSETRKEVDDSIEKWGKTPIKHVYDLGLFERPTIAAHCVHV
                     NDEDIQILSESGVSVAHNPSSNLKLASGFAPVDKMLKAGVNVALGTDGASSNNNLNMF
                     EEIHLASIIHKGANFDAVSVPAEDVIKMATVNGAKAFGLEEELGSIEVGKKADIILID
                     LRKPHFAPLHNPVSAVCYSAQGSDVHTVIVDGKILMENYELKTIDVEKAIYEAAEAAK
                     KLVGA"
     gene            1211..1987
                     /locus_tag="PK768_09075"
     CDS             1211..1987
                     /locus_tag="PK768_09075"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HQA61162.1"
                     /translation="MRKAIIGGTGVYDLGETASREVITEYGTVEVDILNIKGEEIAFL
                     PRHGKGHSIPPHLINYRANLMALKELGVKYIYASCAVGSMNENFAPGEAVLIKDFIDF
                     TKKRPLTFYEGGKRGVAHVPMDEPYCPNLRKKFVKHAKKTGLDIKGEAVYVCTEGPRF
                     ETASEIRMFQRLGADVVGMTNVPEVVLANELGMCYQAVAIITNWCTGFKGMLLDHDIK
                     GIMEENKELITSTFISVLTEEELTQDECNCENPLLIMGLI"
     gene            2027..>2785
                     /locus_tag="PK768_09080"
     CDS             2027..>2785
                     /locus_tag="PK768_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQA61163.1"
                     /translation="MKKLSLATRIFIGLVLGVLTGLLLQSSPEVATTYIKPLGNLFLN
                     LIKMIIVPLVLSSLVVGSASTGDVGKLGRIGIKTLIYYLVTTALAVILGLFLANLINP
                     GHGLSIPLDSTAEAREAPSIVETLLNIVPTNPIQAMVEANMLQIIFFALFLGIGITLV
                     GDRAKPLLSFFDSLAEVSYKIVAIIMAFAPIGVFGLIAPVVAEHGASALLPLLKVIIA
                     VYIACFLHAVFVYSAAVYFSAKMSPVTFFKGAAPA"
BASE COUNT          824 a          572 c          672 g          717 t
ORIGIN      
        1 aaatttccca aatcggcaaa atcaaaacag aatttgagtt tcaaaagatc atcaacgcaa
       61 gaaacatgct agccatgccg ggcctcatca atgcccacac ccacctggcc atggttctgt
      121 ttagaaacta tgccgatgac atgccgcttt tcgattggct tacgaaaaag atatggcccc
      181 tggaggcaaa tttgactccc gaggcaatct acagcggctc catcctagga attgcagagc
      241 tcataaagag cggcgtaacg ggatttctgg acatgtactt ttttgccgac gagaccataa
      301 gggcagccct ggaaacgggt gtcagagtat atatcgcaag aggcttgacc gatgaagagg
      361 aaggcaagga agttcaactc gaggaaacca ggcgcctctt taacgaatac cacgaatggg
      421 atggcaaagt taaaatattc gcagggcccc atgcgcccta cacatgcagt cctaggtacc
      481 tcaaaaaagt tgtagagctg gcgcaggaac ttgatatagg aatacacatc cacctttccg
      541 aaactcgaaa ggaagtagat gatagcatag aaaaatgggg caagactccg ataaagcacg
      601 tctacgactt ggggcttttc gaaaggccta ctatagccgc ccattgcgta cacgtgaacg
      661 atgaagacat acaaattctc tcagaatccg gggtcagcgt ggcacataac cccagcagca
      721 acttaaagct ggccagcggc tttgcaccgg tagacaagat gctaaaagcg ggtgtaaacg
      781 tggccttagg tacagatgga gcatcttcca acaacaacct aaatatgttt gaagaaatcc
      841 atttggcatc tataatacat aaaggcgcaa atttcgatgc ggtgtctgtc cccgcagaag
      901 atgtcataaa aatggccaca gttaatggag ctaaggcctt tggccttgaa gaggaactag
      961 gaagcattga agttggtaaa aaggcagata ttattttgat tgatctgaga aagccgcatt
     1021 ttgcaccgct ccacaatccc gtttcagcag tctgttactc tgcccaaggc tcggatgtgc
     1081 atacggttat cgtagatggt aagattttaa tggaaaacta tgaattaaaa acaatagatg
     1141 ttgagaaagc tatttacgaa gcagctgaag ctgccaaaaa actagttggt gcttaaggag
     1201 gtgttttttt atgaggaaag ccatcatagg cggtacaggt gtgtatgatt taggcgagac
     1261 tgcttctcgc gaagtaataa ccgaatacgg aacagtagag gtagacatat taaacataaa
     1321 gggtgaggaa attgcctttt taccgaggca cggtaagggc cattcgattc cacctcatct
     1381 cattaattat agagctaact tgatggcatt aaaggagtta ggagtaaaat acatttatgc
     1441 aagttgcgct gtaggttcca tgaatgaaaa ctttgctccc ggtgaggcag tgctgatcaa
     1501 agactttata gacttcacta aaaaacggcc cctgacattt tatgaagggg gtaagagggg
     1561 cgtagcgcac gtgccaatgg atgagcctta ctgccctaat ttgaggaaaa agttcgtgaa
     1621 gcatgcgaaa aagactggtc tcgatataaa aggtgaggct gtatatgtgt gcacagaagg
     1681 accaaggttc gaaacagcca gcgaaatccg tatgtttcaa aggctcggcg ctgatgtagt
     1741 gggcatgaca aatgtgccgg aggtggtcct ggcgaatgaa ctgggcatgt gctatcaggc
     1801 agttgcaata atcacaaatt ggtgcacggg cttcaaaggc atgctactgg accatgacat
     1861 aaagggcata atggaagaga ataaggaact tattaccagc acctttattt cagtacttac
     1921 cgaggaagag cttacgcaag atgaatgcaa ctgtgagaac ccgctgttaa ttatgggctt
     1981 aatctaaatc agggggcgat atgaagtcgc aaagggggat cgttttttga aaaagcttag
     2041 tttagccacg agaatcttca ttggcttggt actaggtgta cttacgggtc ttttgcttca
     2101 atcgtcaccg gaagttgcga caacttatat aaagcctctg ggaaatcttt ttttaaatct
     2161 cattaaaatg attatcgtcc ccttggtact gtcctcctta gttgtgggca gtgccagcac
     2221 cggtgatgtc ggaaaacttg gaagaatcgg cataaaaaca ttaatctatt atctggtcac
     2281 tacggcttta gcagtaatat tgggtttgtt tcttgcaaac ttaataaatc ccggccacgg
     2341 attgagcatt cctcttgatt caacagcaga agcaagagaa gcgccttcaa tagtcgaaac
     2401 cctcctcaat atcgtcccaa caaacccaat tcaggctatg gttgaggcca acatgcttca
     2461 aatcatattt ttcgcactat ttctaggaat tgggataact ttagtgggcg atagggccaa
     2521 accgctccta agtttttttg atagtttggc agaagtgtct tataaaattg tggccataat
     2581 aatggcattt gccccgatag gagtttttgg tttaattgct cccgttgtgg cagaacacgg
     2641 ggcatcggca ctgctgcctc tgttaaaagt gataattgca gtttatatcg cttgtttcct
     2701 tcatgctgtc tttgtttatt cggctgcagt ttatttttct gcaaagatga gccccgtcac
     2761 attttttaag ggggcagccc ctgcc
//
LOCUS       DAONUO010000277         2754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_25064_length_2754_cov_3.439422, whole genome shotgun sequence.
ACCESSION   DAONUO010000277 DAONUO010000000
VERSION     DAONUO010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25064_length_2754_cov_3.439422"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PK768_09085"
     CDS             <1..973
                     /locus_tag="PK768_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQA61164.1"
                     /translation="TRIPKVAETQESVIPVIAREVSPAVVGISTIHVEYDFFYRPVET
                     EAVGSGVIVDTSGHIITNDHVVGNADQITVFLSDGRKREAIRLYGDPSLDLAIIKIDA
                     PNLAAARLGDSDSVLVGDLAVAIGNPLGLSLQRTVTAGIISALNRMVVIRDRRGEVLM
                     QDLIQTDASINPGNSGGPLVNSKGEVIGINTVKASRAEAIGFAIPVNIVKPIVRSVIE
                     KGSFDKPYLGIEGIDREIAQVMNLKVTVDSGVYVAGVERGSPADKAGIKTADIITKLA
                     GKKVGSMAKLRQIIYDLDVGKPVDIEIRRNGSILTKTIVPDKAPTSN"
     gene            1469..2308
                     /locus_tag="PK768_09090"
     CDS             1469..2308
                     /locus_tag="PK768_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HQA61165.1"
                     /translation="MKMNLRNLVLIMLILAVGVAGLAGCSSQSASQAPSQEEEAVVED
                     SFDKIKERGYVIMGLDDTFAPMGFRDENNEIVGFDVDLAKEVFARNDLELVLQPIDWT
                     MKEAELNAGNIDMIWNAYTITAERKEKVAFTEPYLDNRQVIVTLADSDINSKKDLEGK
                     KVVAQAGSTAVDAMYNEPDVVATFYGGEPILFDTNHEAFMDLEAGRSDAIVADEVLAR
                     YYIKQKGPEKYKVLDEDFGDEEYGIGFRKQDKKLLEMVESTLDEMRKDGTFDAIYDKW
                     FAD"
     gene            2334..>2754
                     /locus_tag="PK768_09095"
     CDS             2334..>2754
                     /locus_tag="PK768_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HQA61166.1"
                     /translation="MVDYILRILPALLAGLDTTLSVFFLTLVLSIPLGVLVALGRLSN
                     IKPLVEIMKFYIWVMRGTPLLLQIVVIFFGLPIVGITFDRYPAAIIAFVLNYAAYFGE
                     IFRGGIESIDKGQYDAAKVLGFTPIKTYQRIILPQMIK"
BASE COUNT          849 a          531 c          664 g          710 t
ORIGIN      
        1 aacccggatt ccgaaagttg ccgagaccca ggaatccgtc ataccggtaa tagctcggga
       61 agtttctccg gctgtggttg gaatatcaac cattcacgtc gaatacgatt ttttttacag
      121 accagttgaa acggaagcgg taggttccgg tgtcatagtt gacacttcag ggcacattat
      181 caccaatgac cacgtagtgg gaaatgccga ccaaatcacc gtgtttctat cggatggcag
      241 gaaaagagaa gcaatcaggc tctacggaga cccatccctt gacctggcaa taataaaaat
      301 agatgctcct aatttggcag cggcaagact cggagattcg gattcagtcc ttgtgggaga
      361 tttagcggtg gccataggaa acccattagg cctgagcctt cagaggaccg ttaccgcggg
      421 tataatcagt gctcttaaca gaatggtggt aataagggac cgcagaggtg aagtattaat
      481 gcaggacctc atacagacgg atgcttccat aaatcccggc aacagcggcg ggcccttggt
      541 caacagcaaa ggggaagtta taggcatcaa cacggtcaaa gcatcccggg ccgaggcgat
      601 aggctttgcg atacccgtga atatagtgaa acccatcgta aggagtgtaa tagaaaaagg
      661 cagctttgac aaaccctatc tgggcataga aggcatcgac agggagatag ctcaggtcat
      721 gaatcttaaa gtaacggtag acagcggagt ctatgttgca ggtgtggaaa ggggtagtcc
      781 agcagataag gcaggcatca aaacagcgga tataataact aaacttgccg gtaaaaaagt
      841 gggttcaatg gccaagctgc ggcagattat atacgatcta gatgtgggaa aaccggtaga
      901 tatagaaatc cggcgcaatg gaagcatact aacaaaaacg atcgtgcctg ataaagctcc
      961 tacttccaac tgaattatta ggaataacag gctttgccta taggatggct gccgtcatcc
     1021 aggctcatta catttgtgcg tattgatgag ggtatgcctc cttgcgcata ataaatcatt
     1081 cttgagtttt gcttcttcaa tatacagggt taccgttagc gtacacttcc cccaatctat
     1141 ttttttgcct taagtgacag cacaccgtct tccgccatca ttgcatctct agctagactt
     1201 aacaatggcg cacaacaatt agaaagcaat ttaccatcct ccgagtgcca aaccttagcc
     1261 ctgaacgctg acacttccat tgtctagtcc aataaagatt ggtttaaatg attcggaaca
     1321 gcaacatata taacattata attagtattg cgcaaatatt gaagaagctg gctacctgaa
     1381 aatagcggtt gacaaaatca ctttaacact ttatcatatt aaagtagtga aacaagatta
     1441 tgtagataaa gaaaaggaga gaatgatgat gaaaatgaat ctgaggaatt tagttttgat
     1501 aatgcttatt ttggctgtgg gggtggccgg tttggcagga tgctcgagtc aaagcgcaag
     1561 tcaagctcca agtcaagaag aggaggcagt agttgaggat tcctttgata agataaaaga
     1621 gagaggctat gttatcatgg gccttgacga tacatttgct cccatgggtt ttagagatga
     1681 aaacaatgaa attgtaggtt ttgacgtaga tttggccaag gaagtttttg caagaaatga
     1741 tttagagttg gttttgcaac ccattgactg gacgatgaaa gaggctgaat taaacgcagg
     1801 caacatcgat atgatttgga atgcatacac cattactgcc gagcgaaagg aaaaggttgc
     1861 ttttaccgaa ccctatctgg acaacaggca ggttatcgtt acacttgctg attccgacat
     1921 aaacagcaaa aaagaccttg aagggaaaaa agtagttgcc caagcaggtt caacggccgt
     1981 agacgccatg tacaacgaac ctgatgtagt tgcaacattt tacggtgggg aaccgatact
     2041 ctttgataca aatcacgaag ctttcatgga tttagaagca ggaagatcgg atgccattgt
     2101 agctgatgaa gttttggccc gctattacat taagcaaaag ggtcctgaga agtacaaagt
     2161 tcttgacgag gattttggcg atgaagaata cggcataggc tttagaaagc aggacaagaa
     2221 actccttgaa atggttgaaa gcacattaga tgaaatgaga aaagacggaa cctttgatgc
     2281 gatctatgac aagtggtttg cagactaaat aataaatgta aagggaatct gttatggtag
     2341 attatatact tagaatttta ccggccctgc ttgcagggct tgatacaact ttaagcgtat
     2401 ttttcctgac actggtgctt tcaatccctt tgggagtttt ggtggcactg ggaagacttt
     2461 cgaatataaa gcccttagtt gaaattatga agttttacat ttgggtaatg cgggggacgc
     2521 cccttttact tcaaattgta gtcatatttt ttggcctgcc catagttggc ataacctttg
     2581 acagatatcc cgctgccata attgcttttg tattaaatta tgcggcgtac tttggcgaaa
     2641 tcttcagagg tggcatagag tcaatagaca agggccaata tgatgcggcc aaagtcctgg
     2701 gtttcacccc tataaagaca taccagcgga ttatattgcc gcaaatgatt aaaa
//
LOCUS       DAONUO010000278         2745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_25169_length_2745_cov_3.575836, whole genome shotgun sequence.
ACCESSION   DAONUO010000278 DAONUO010000000
VERSION     DAONUO010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25169_length_2745_cov_3.575836"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /gene="uvrA"
                     /locus_tag="PK768_09100"
     CDS             <1..1541
                     /gene="uvrA"
                     /locus_tag="PK768_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQA61167.1"
                     /translation="QVTALSIREALKFFNELCLSELEKMIAHQVLKEIKNRLLFLVDV
                     GLDYLTLDRAAGTLSGGESQRIRLATQIGSRLVGVLYILDEPSIGLHQRDNDKLLKTL
                     KDLRDLGNTVLVVEHDEDTMRTADYIIDIGPGAGEHGGRIVACGPIEDIEKSEESITG
                     QYLSGKKQIPIPKKRRKPNEAWLELKGCREHNLKNIDVRFPLGLFTCVTGVSGSGKST
                     LVNEILYKVLREKLYKARTFPGDFDEILGIEHVGKVINIDQSPIGRTPRSNPATYTNV
                     FNDIREVFSLTPEARMRGYKPGRFSFNVKGGRCEACKGDGIIKIEMHFLADIYVPCEV
                     CKGKRYNRETLEVKYKGKSIADILDMTVDEALEFFENIPKIKRKLETLRDVGLGYIRL
                     GQPSTTLSGGEAQRVKLSTELSKLSNGNTVYILDEPTTGLHPADIHKLLDVLQRLVEG
                     GGTVIVIEHNLDVIKTADYIIDLGPEGGDKGGQIVATGTPEEVAQNPNSYTGQFLKKV
                     LNRQ"
     misc_binding    1753..1969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2019..>2745
                     /locus_tag="PK768_09105"
     CDS             2019..>2745
                     /locus_tag="PK768_09105"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQA61168.1"
                     /translation="MREDLIQLKERTLEALSNLQNMDELNDLRVKILGKKGELTQVLR
                     GMAKLTAEERPVIGKLANEIKDELESKFSEKMRLIKKAVQEQKMQQEFIDVTIPTKIS
                     VGSKHPLTLVLDEIKEIFIGLGYEVAEGPEVELDYYNFEALNTPADHPARDVQDTFYI
                     TEKILLRTQTSPVQVRTMEKQSPPLKIIAPGRVYRSDSVDATHSPMFHQVEGLTVGEG
                     FTMGDLKGTLVKFVHEMFGRNRKA"
BASE COUNT          796 a          608 c          730 g          611 t
ORIGIN      
        1 cccaggttac tgccctttcc attcgtgagg ccttaaagtt tttcaatgag ctttgcctga
       61 gcgaattgga gaaaatgata gcacaccagg tcctaaagga gataaaaaac cgcttgctgt
      121 ttttagtaga tgtgggtctt gactatctga cccttgacag ggcggcggga acattgtcag
      181 gcggtgagtc tcaaaggatc aggcttgcca ctcaaatcgg ttcccgatta gtcggcgttc
      241 tgtacattct cgatgagccc agcatagggc tgcaccagcg ggacaacgac aagttgctga
      301 agaccttaaa ggaccttagg gatttgggca ataccgtgct ggtggtggaa cacgatgaag
      361 ataccatgag gactgccgac tacatcattg acatcggtcc cggggcagga gaacacggcg
      421 gcagaattgt tgcctgcggg cccatagaag acatagagaa aagcgaagag tccatcacgg
      481 gccagtatct gagcgggaaa aaacagattc cgattcccaa aaaaaggcga aagccaaatg
      541 aggcatggct ggaactcaag ggctgcaggg agcacaattt gaagaacata gatgtaagat
      601 ttcccttagg gctatttacc tgcgtaaccg gcgtttccgg ttcgggcaag agcaccttgg
      661 ttaatgaaat cttatacaag gtcttaagag aaaaactcta caaagcccgg acttttccgg
      721 gggatttcga tgaaatactg ggtatcgagc atgtgggcaa ggtcataaat atcgaccaat
      781 cgcccatagg gaggacgccc cgctcgaacc ccgccacata tacaaatgtg ttcaatgata
      841 tcagggaggt cttctccctg actcccgaag cccgcatgcg cggctacaag cccggcaggt
      901 ttagcttcaa tgtaaagggc ggcaggtgcg aggcctgcaa gggtgatggc attataaaaa
      961 tcgagatgca ctttttggcg gacatctatg tcccctgcga agtctgcaaa gggaagcgct
     1021 acaacaggga gaccctggaa gtgaaataca agggcaaaag catagccgac atactggata
     1081 tgaccgtcga tgaggccttg gaattctttg aaaacatacc caagataaag cgcaagcttg
     1141 agacgctgcg ggatgtgggt cttggctata tcaggctggg acagccttca accaccttgt
     1201 ccggtggtga agctcaaagg gtcaagctgt ctacggaact ctcaaagctg agcaacggca
     1261 ataccgtcta catcctggac gagcccacaa ccggcctgca ccctgccgat attcacaaac
     1321 tccttgatgt acttcaaagg ctggtggagg gtggaggcac ggtcatagtc attgaacaca
     1381 acctggatgt gataaaaact gcagattata taatcgattt gggtccggaa ggaggagaca
     1441 aaggaggtca aattgttgcc acgggcacgc cggaagaggt ggcccaaaac cccaactcct
     1501 ataccggtca atttctgaaa aaagtgctta acaggcagtg agattaaaaa atgtgcaaac
     1561 tgatttcccg gcctatacgc ctgcttacca tgcacaatct ttctcttgta aatagctttt
     1621 tgctgtgcta taatatgtgt aaaccttagc ggaaagggcg tggccaatgc tgaccgccga
     1681 ctttttttgg aacatttttg aggtaaccgg atccattacg gcctatctta tttatagaca
     1741 aatgttgcca tcgtaaatgc catgaaggag agagtaagcg ggggagcctt gcagggagag
     1801 agggtcgcgg actgcaagcc ctcttaaggc agaagccgcc gaagttcact ctggagcagg
     1861 ggggctgaat tcagtaggtc tccacggttt caccgttata gcacaaagag agtttggtta
     1921 tcaaactgaa caagggtggt accacggaag aaaagctttc gtccctagct ggacgaaagc
     1981 tttttacttt acttaaatat tcgcaggagg tatctgagtt gagggaagat ttgattcaat
     2041 taaaagaaag gactttggaa gccctatcta atttacaaaa tatggatgaa cttaacgatt
     2101 taagagtaaa gattcttggg aaaaaaggtg aacttaccca agtgcttcgg gggatggcca
     2161 aactgactgc tgaggaaaga ccggtcatcg ggaaactggc caatgagata aaggacgagc
     2221 tggaaagcaa atttagcgaa aagatgcggt taataaagaa ggctgtgcag gaacaaaaga
     2281 tgcagcagga gttcatagac gtaacgattc ccactaaaat ttcagtaggc agcaagcatc
     2341 cccttacttt ggtcctggat gaaattaaag agatctttat aggtctcgga tatgaagtgg
     2401 cagagggacc ggaagtggaa ctcgattact acaatttcga ggccttaaat acaccggcgg
     2461 accatccggc cagggacgtc caggacacct tttacatcac tgaaaaaata ctcttgagga
     2521 cccagacatc cccggtccag gtgaggacca tggaaaaaca aagtcccccc ttgaagataa
     2581 tagccccggg cagggtatac cgctccgatt cggtagatgc tacacattcc cccatgttcc
     2641 atcaggtgga aggccttacc gtgggggaag gttttacaat gggtgatctc aaggggaccc
     2701 tggttaaatt cgtgcatgaa atgtttggcc gcaatcgaaa ggccc
//
LOCUS       DAONUO010000279         2726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_25372_length_2726_cov_2.422688, whole genome shotgun sequence.
ACCESSION   DAONUO010000279 DAONUO010000000
VERSION     DAONUO010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25372_length_2726_cov_2.422688"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2726
                     /locus_tag="PK768_09110"
     CDS             <1..>2726
                     /locus_tag="PK768_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQA61169.1"
                     /translation="FIIVDRSVVNASWGQNVWTDRHQIITSLDFAKQDDVMFALKDSS
                     WDLVIVDEAHKMSAYKYGEKINKTARYQFGELISQITKYLLFLTATPHRGDPENFRLF
                     LDLLQPGFFANTEMLVDSIQNRDNPLFLRRLKEDLEGFDNAPLFPPRKVETLKYRLSD
                     NEKLLYNKVTEYVEKYFNKALQKEKRNVTFALTILQRRLASSVRAVRKSLERRWNRLE
                     ELYKKGQVLQEVEYTEDYLEDLEENERWQKEEELLEKLTSAETLDELKIEIDMLNNLV
                     QLARDVEKQEIETKLNRLREVIENERIKETGTKILIFTESRDTLEYLAEKIGKKWGYQ
                     VTTIHGGMDMDARIRAEHEFKNSAQVMIATEAAGEGINLQFCWLMVNYDIPWNPNRLE
                     QRMGRIHRYGQRNEVHIYNLVAVDTREGRILNKLFEKLNRMKEHLGSDRVFDVIGDVL
                     PGVSLKDLIIDAIANRRTMEDILAEMDRVPDNDTIQRVREATMEALATRHIDLSRIIG
                     EQRLARENRLVPEYIERYFLRAAQKLELNIEKRKDGFWRVSFVPFEMRNQPYEYKIKY
                     GEVQREYLKVSFDKDEAHKGEAEFVSIGHPLMEAVLETVFKKYRQDASSGAIFVDPDG
                     KKNGVLWFMIGEIKDGKGDVAGRKLFCVYQDSNGQLIDVNPSLIWDLKPGKNDSHDLD
                     NSLLMSQNDVIAFALEQQLIPYRDELLKQRQRDAELKEKYGLRSIEQLILESEYKLID
                     YETRRLKGEKIPDATMANEQRNREDLERKKEGLLDDIRAETSLYASEPALLATVRVVP
                     GKSITDEMTSDTTIEQIGMAMAMKYEREQGRQPEDVSTHALGYDIRSVDREGNYRYIE
                     VKARAKSGAIALTPNEWVMANRLGEEYWLYVVEQAAGNPQLYLIKNPAASLQP"
BASE COUNT          861 a          536 c          673 g          656 t
ORIGIN      
        1 tttataattg tcgaccgcag cgtagtaaat gccagctggg gccaaaatgt atggactgat
       61 cgccatcaaa tcataacttc tcttgatttt gcaaaacagg atgacgtcat gtttgctctt
      121 aaggattctt cctgggacct ggtgatcgtg gatgaagccc ataaaatgtc cgcatacaaa
      181 tacggggaaa agatcaacaa aaccgctcgc taccagttcg gcgagctaat ttcccagatt
      241 actaaatatc ttctttttct taccgctaca ccgcatcgcg gagaccctga aaatttccgc
      301 ctctttttag acctgctaca gcctggattt tttgcaaata ccgaaatgct ggtagactcg
      361 attcaaaaca gggataatcc tctttttttg aggcggctta aggaggacct tgaaggattt
      421 gacaatgctc cccttttccc tccccgcaag gtagagacgc tgaaataccg actgagtgat
      481 aacgaaaagc tgctctataa caaggtaacc gaatatgtgg agaagtattt taataaggcc
      541 ttacaaaaag agaaacgcaa tgttaccttt gccttaacta tactacagcg aagactggcc
      601 tccagtgtaa gagcagttcg taagtcttta gagcgccgtt ggaatcggct ggaagaattg
      661 tataaaaagg ggcaggtcct ccaagaggtc gaatatacag aagattattt ggaagacttg
      721 gaagagaacg aacgatggca aaaagaagaa gagctactag agaaactgac ttcagcggaa
      781 actctggatg aactgaagat tgaaatcgat atgttaaata acctcgttca acttgcaagg
      841 gatgttgaaa agcaagaaat tgaaactaaa cttaaccggt taagggaagt tattgaaaat
      901 gaaaggatta aagaaaccgg tacaaaaatt ttgatcttta cagaatccag ggatacattg
      961 gagtacctgg cagaaaagat aggtaaaaaa tggggttatc aagttaccac tattcatggt
     1021 ggaatggaca tggatgcccg tatcagggcg gagcacgagt ttaagaacag tgcccaggta
     1081 atgattgcta ctgaagcagc cggtgaaggt ataaatcttc aattctgctg gttaatggtc
     1141 aattacgaca tcccatggaa ccccaaccgc ctggaacaga ggatgggacg tattcaccgt
     1201 tacgggcagc ggaacgaagt ccatatttat aaccttgtgg cagtagatac cagagaagga
     1261 agaattttaa ataagctctt tgaaaaacta aaccgcatga aagaacactt aggatctgac
     1321 cgggtctttg atgtcattgg tgatgtccta ccaggggtta gcctgaagga ccttataatt
     1381 gatgctattg ccaaccgccg aactatggaa gatattctgg ccgaaatgga tcgggtccca
     1441 gacaatgaca caatccaaag agtcagagag gcaaccatgg aggcattggc tacccgtcac
     1501 atagatcttt ccagaatcat cggagaacag cgccttgctc gagagaatcg tttagtaccc
     1561 gagtatatcg aaagatattt tttgcgtgct gcccaaaagt tggaattaaa tatagaaaag
     1621 cgcaaagacg gcttttggcg ggtaagtttt gttccttttg aaatgcgcaa tcagccttat
     1681 gaatacaaga taaaatacgg tgaagtgcag cgggagtacc tgaaagtttc ctttgataaa
     1741 gatgaggcac ataaaggcga agctgaattt gtctccattg gtcatccctt gatggaggca
     1801 gtattggaaa ccgtctttaa gaaatatcgg caggacgcga gcagcggagc tattttcgtt
     1861 gacccagacg gtaaaaagaa cggagtactc tggtttatga taggagagat aaaagacgga
     1921 aagggcgatg tagccggacg caaactgttt tgtgtttacc aggatagtaa tgggcaactg
     1981 attgatgtta acccctctct tatttgggac ctaaaaccgg gtaaaaatga ttcccatgat
     2041 ttagataata gtcttcttat gtctcagaat gatgttattg ctttcgcatt agagcagcag
     2101 ttaattcctt atagagatga actcctcaag caaaggcaaa gggatgccga acttaaagaa
     2161 aaatacgggt taaggtctat tgagcagttg atcctcgaat cggaatataa gttgattgac
     2221 tatgaaacaa ggaggctcaa gggtgaaaaa attccggatg caacgatggc caacgaacag
     2281 agaaatcgtg aagacttgga gcgaaaaaag gaggggctat tggatgacat ccgtgctgaa
     2341 acaagtctct atgcgtcaga gccggctctc cttgccacgg tccgtgtggt accagggaag
     2401 tctataactg atgaaatgac cagcgataca actattgagc agataggcat ggccatggcc
     2461 atgaaatatg agcgggaaca gggaaggcag ccggaggatg tgtcaacaca tgccctaggc
     2521 tatgacatcc gctctgttga cagggaaggg aattatcgct acattgaagt taaggcccga
     2581 gcaaaaagcg gggccatcgc cctaacaccc aatgaatggg taatggctaa ccgactgggt
     2641 gaagaatact ggctttacgt tgtagagcag gcggctggga acccgcagct atacctgatc
     2701 aagaacccgg cagcttctct acaacc
//
LOCUS       DAONUO010000280         2692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_25767_length_2692_cov_4.291240, whole genome shotgun sequence.
ACCESSION   DAONUO010000280 DAONUO010000000
VERSION     DAONUO010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25767_length_2692_cov_4.291240"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PK768_09115"
     CDS             <1..238
                     /locus_tag="PK768_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA61170.1"
                     /translation="KKYNLTTVLWCVDPRDWQETDPDVIARHVIDRATNGAIILLHEG
                     RESTIEALPQIIEGLWERGFEIVSLSELLSEEIV"
     gene            363..800
                     /locus_tag="PK768_09120"
     CDS             363..800
                     /locus_tag="PK768_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQA61171.1"
                     /translation="MEKVNPAFSFDTIKRGFRSGLNITWKLAKILVPVYFIVTILKYT
                     PIINWISAAFAPLMRVFGLPGEAAIVLVMGNVVNLYAAIGAMASLDLSIKEISILAIM
                     LSFCHSLIVETTLAKKIGLSALKVISVRVGLAVVSGIAFNLFL"
     gene            817..1275
                     /locus_tag="PK768_09125"
     CDS             817..1275
                     /locus_tag="PK768_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQA61172.1"
                     /translation="MSSILMEAAIGSFNSVKQIAIIVIPIMIGLEILKDIGILDKLTQ
                     LFSPLVKIYGMKKEAGFPLVIGIVIGLSYGAGIIFQAAEEDKLSRRDLYLLTYFLVAA
                     HAVFEDTAVFMALGVNGILLFVVRVTVAAFFTYLASRWIKVDDLDFEKMR"
     gene            1283..1609
                     /locus_tag="PK768_09130"
     CDS             1283..1609
                     /locus_tag="PK768_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HQA61173.1"
                     /translation="MESMANCDYKVISLPKLNGLTPTLESTALKLMEEAGELAQAIGK
                     FRGQSGEAIQMDEKQVVDKMAQELLDVAQVAVSMMFVLEENYDIDIEQKLKQHIEKLK
                     SKGYIK"
     gene            complement(1610..>2692)
                     /gene="rho"
                     /locus_tag="PK768_09135"
     CDS             complement(1610..>2692)
                     /gene="rho"
                     /locus_tag="PK768_09135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQA61174.1"
                     /translation="RIENYLPSDEDIYISPSQIRRFHLRTGDMITGSIRPPKEGEKYR
                     AVLQITAVNGLDPEHAIRRFHFDSLTPIFPQPRFVMEYDPQELSTRIIDIIAPIGKGQ
                     RGLIVSPPKAGKTMMLKNIANSLAKNHPDVQLIVLLIDERPEEVTDIRRSVEGEVVSS
                     TFDELPENHLRVADMVLERAQRLVESGKDVVILLDSITRLARANNLVVPPTGRTLSGG
                     LDPSALHKPKRFFGSARNIEEGGSLTIIATALVETGSRMDDVIYEEFKGTGNMEIHLD
                     RKLAERRIYPAIDIKKSGTRREELLFSKEELEATWVLRKALAPLDTVEASSIFINKLK
                     KTKTNREFYENLAAMVNDLQNNRETL"
BASE COUNT          671 a          600 c          626 g          795 t
ORIGIN      
        1 caaaaagtat aatctgacca cggttttatg gtgcgtggac ccccgggact ggcaggagac
       61 tgacccggac gttattgcac gacacgtgat agatcgtgcc acaaacggcg ccattatcct
      121 gcttcacgaa ggtcgagagt cgacaatcga ggctttgccc cagatcatag aaggcctttg
      181 ggaaaggggt ttcgagatag tatctttatc agaactcttg tccgaagaaa tagtatgagt
      241 gcacgaatga ataattgggg actgtccttt tttattcata gcttattaac tgctctcttt
      301 tacgctaaaa agattcatgt tataatagtc caagagattt tttgaggata ggaggagttt
      361 ttttggaaaa agtaaatcct gctttttctt ttgatacgat aaagcgcggc tttaggtcag
      421 gtctcaatat tacctggaaa cttgctaaga ttttggtgcc tgtttatttt atagtcacca
      481 ttttgaaata cacccctata atcaactgga tttctgctgc ctttgcccct ctcatgaggg
      541 tgtttggtct ccccggtgaa gcagcaatag tactcgtaat gggcaatgtt gtaaatctgt
      601 atgccgccat tggggctatg gcttctctcg atttgtcgat taaagaaata tctattctag
      661 cgataatgct ctccttttgc catagcttaa tagtagagac gacattggcc aagaaaattg
      721 gcctgtcagc acttaaagta atttcggttc gagtgggact cgctgtcgtt tcaggaattg
      781 cttttaatct ctttttgtaa gtattggagg taagccatgt caagtatttt aatggaagct
      841 gctattggca gttttaactc cgttaaacag attgccataa tcgtaattcc cataatgatt
      901 ggattggaga ttttaaagga tatcggtatc cttgataaac tgacccaact gttttcgccc
      961 ttggtaaaaa tatacggcat gaaaaaggaa gcaggcttcc ccctagttat cggcatcgta
     1021 ataggcctgt cctacggtgc cggcatcata tttcaagcag ccgaagagga caagctcagc
     1081 cgccgtgacc tctacctgct tacgtacttt ttggtggcag cccacgccgt ttttgaagat
     1141 accgccgttt ttatggcctt gggtgtaaac ggcatcttgc tttttgtcgt ccgcgtgacc
     1201 gtggcagctt tctttaccta tcttgcctcc aggtggataa aagttgatga cctggatttt
     1261 gaaaaaatga ggtgatgcga agatggaatc gatggcaaat tgtgattaca aagttatcag
     1321 cttgcccaag ttaaacggcc ttactcccac acttgaatca acggcgttaa aattaatgga
     1381 agaggccggt gaactggccc aagcaatagg aaaatttcgc ggtcagagcg gtgaagctat
     1441 ccagatggat gaaaagcagg tagttgataa aatggcccag gaactcctcg atgtcgccca
     1501 ggttgctgtt tcgatgatgt tcgtcctcga ggagaattac gatatagaca tcgaacaaaa
     1561 attgaagcaa catatagaaa aactgaagtc taaaggctac atcaaatagt taaagagttt
     1621 ctctgttgtt ctgcaggtca tttaccatgg cagctaagtt ttcgtaaaac tccctgtttg
     1681 tcttggtttt ctttagtttg tttatgaata tactggacgc ctcaacggta tccaacgggg
     1741 ccaaggcttt tctcaaaacc catgtggctt ccaattcttc tttgctaaac agcaactcct
     1801 cccggcgcgt accggatttt tttatgtcaa tggcgggata aattctgcgc tctgcaagtt
     1861 ttctatcaag gtgtatctcc atattgcctg ttcccttaaa ttcctcatag atcacatcgt
     1921 ccatcctgct gccggtttcc accagtgcag tagcaattat agtgagactg ccaccttcct
     1981 caatgttccg tgcggaacca aaaaagcgct ttggcttgtg aagagcactg gggtcaagac
     2041 cacccgagag ggttcttccg gtgggcggaa caaccaggtt atttgctcgc gccaggcggg
     2101 ttatgctgtc gagcagtatt accacatcct tgccgctctc caccaagcgc tgagcccttt
     2161 ccagaaccat atcagccacc ctcaagtgat tttccggcag ctcgtcaaat gtcgaactta
     2221 caacctcacc ttccacggag cgccttatgt ccgtcacctc ttccggcctt tcatcgatta
     2281 acagcactat cagctgtaca tccggatggt tcttggcgag gctgttggct atatttttca
     2341 gcatcatggt ctttcctgct tttggtggag atacaataag gcctctctgg cctttcccta
     2401 tgggggcgat tatgtcgata atgcgcgtag aaagttcctg cggatcgtat tccataacga
     2461 aacggggctg cggaaaaata ggtgtcaggg aatcaaagtg aaatctgcgg atggcatgtt
     2521 ccgggtcaag gccgttgact gcagtgattt gcagcaccgc cctatacttt tccccttcct
     2581 tgggaggtct tatgctccct gtgatcatat caccggttct caggtgaaag cggcgaatct
     2641 gggatggaga tatgtatatg tcttcatctg atggtaggta gttttcgatt ct
//
LOCUS       DAONUO010000281         2675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_25940_length_2675_cov_3.935115, whole genome shotgun sequence.
ACCESSION   DAONUO010000281 DAONUO010000000
VERSION     DAONUO010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25940_length_2675_cov_3.935115"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PK768_09140"
     CDS             <1..884
                     /locus_tag="PK768_09140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA61175.1"
                     /translation="SFRSDSYIIISMIIFASLLIQFGLPFLEKKMKPKLLYDIGVGLG
                     VLGFLILLYFNKYVYRGFGLQTDIIRHGTHHFKYVALTFDDGPDPVYTPEILDILKEK
                     DVRATFFLVGKNVNSYPEIAKRIVEEGHSIGNHTYSHRSLIPLTAKSTYKEVKDAETA
                     IDEATGVRPTLFRPPRGVYSNYVRGLLKEEGYTLVLWDLSAVDWAELAPKKIVTNVKN
                     RVKPGSIILLHDSGDLITHRGGNRASTVKALPEIIDTLRAQGYEFVTVDQMIFITELM
                     ETEEHSHENNLSPAQAH"
     gene            850..1488
                     /locus_tag="PK768_09145"
     CDS             850..1488
                     /locus_tag="PK768_09145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQA61176.1"
                     /translation="MKIILAQPRHIKMAAEIFCLAFEQSITFFTPISRKVKTAIEDIF
                     YLLHRAFGQGFFVAVENDKLCGYVIMADDIKRLWIKTITSGFLIKAILSAASGKYGLT
                     PSALYKIVKNKLLYFRFEMTTEPSAQVLSIAVHPEHQGRGIGQKLLSKGIEYLEALGI
                     RRIKLEVRPNNISALKIYEKFGFRTVGEAEDLQGKWLIMMRKDCLGENNMLI"
     gene            1536..2429
                     /locus_tag="PK768_09150"
     CDS             1536..2429
                     /locus_tag="PK768_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQA61177.1"
                     /translation="MSRQQNIKISTNAMAQLIAMQQDEITDHVTYKAIARKIKNPENK
                     KILEKIAAEEKKHYEILKRYTNRSLKPEPLKVWWRTLMASILGFTFTIKLMESSEAIA
                     QHNYSDLIKEVPEAEIIVAEEEEHEEKLINMLNEERLQYVGSMVLGLNDALVELTGTL
                     AGLSFALQNTRIIALSGLITGISATLSMASSEFLSAKSEGNKDALKSSFYTGIAYLIT
                     VVMLILPYLLLPPQKYMLALFTMLITVVAIIAAFNYYIAVAKDLPFKRRFLEMATISL
                     SVAVLSFVVGLIVKNALGVDI"
     gene            2587..2673
                     /locus_tag="PK768_09155"
     tRNA            2587..2673
                     /locus_tag="PK768_09155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2621..2623,aa:Ser,seq:tga)
BASE COUNT          824 a          504 c          617 g          730 t
ORIGIN      
        1 aaagctttcg aagtgatagc tatataataa tatccatgat aatctttgct agtcttttaa
       61 ttcagtttgg attgcccttt cttgagaaaa aaatgaaacc gaaattgctt tacgacatag
      121 gagtagggct gggagtcctg ggctttttga ttcttcttta ctttaataaa tatgtctata
      181 gaggctttgg tttgcaaaca gacataatcc ggcacggtac tcatcacttc aagtatgtgg
      241 ctttgacctt tgatgatggt cccgaccccg tatatactcc ggaaattctt gatatattga
      301 aagaaaaaga tgttcgggca actttcttcc tcgtagggaa aaacgtcaac agttatccgg
      361 aaatagccaa aagaatagtg gaagaaggcc actccatagg aaatcacaca tattctcata
      421 gaagcttgat accacttacg gccaaaagca cttataaaga agtcaaggat gctgagactg
      481 ctatagatga agccacaggt gttagaccca ctttgtttcg accaccgaga ggggtctatt
      541 ccaactatgt tcgaggactg ctgaaggaag aaggctatac attggtcctt tgggatttgt
      601 cagctgtgga ctgggcagaa cttgctccca aaaagattgt cacaaatgtg aaaaataggg
      661 tcaaacccgg ctccattatt ttgcttcacg acagcggtga cctgataaca cacagaggag
      721 gcaacagggc ttcaactgta aaggctcttc cggagattat agacacgctg agagctcagg
      781 gatacgaatt tgtgactgtg gatcagatga tatttattac agaactaatg gaaacggagg
      841 aacactctca tgaaaataat cttagcccag cccaggcaca ttaagatggc ggcggaaata
      901 ttctgcctgg cttttgagca aagcataaca ttttttacac caattagccg gaaggtgaaa
      961 acagcgattg aagacatatt ttatttactt caccgcgctt tcgggcaggg attttttgtc
     1021 gcagttgaaa atgataagct ttgcggctat gtcatcatgg ctgatgatat taaaagattg
     1081 tggataaaaa ctataacttc aggctttctc ataaaagcca tattgtcagc ggcaagcggg
     1141 aaatatggtc tgacaccttc ggcgctttat aaaattgtta agaacaaatt actatacttt
     1201 cgattcgaga tgactacgga accttctgcc caggttttat caattgctgt tcatcctgag
     1261 catcaaggta ggggcattgg tcaaaaactg ctctcaaagg gaattgagta tttggaagcc
     1321 ttggggataa ggaggataaa gctggaagtg aggccaaata acatctcggc tctaaaaata
     1381 tatgagaaat ttggatttcg cacagtaggt gaggctgagg atttacaagg gaagtggctt
     1441 attatgatga ggaaggattg tcttggcgag aataatatgt taatatagct agggtctgca
     1501 atcgtttata ttctgtggaa ttggaggtaa ggaatttgag caggcagcaa aatataaaaa
     1561 tatcaacaaa tgctatggcg caacttattg caatgcagca ggatgaaatt acggatcacg
     1621 tgacctataa agccatagct cggaaaataa aaaatccgga aaacaagaaa atccttgaaa
     1681 agattgcggc tgaagaaaaa aagcactatg aaatattaaa gagatataca aatcgcagcc
     1741 taaaacccga gcccctcaag gtgtggtggc ggacgctgat ggcgagtatc ctcggattta
     1801 cctttacgat aaagctgatg gaaagcagtg aagctatagc ccagcacaat tacagtgacc
     1861 ttattaaaga agtgcctgaa gccgagataa tagttgcaga ggaagaagag catgaggaaa
     1921 aactcataaa catgcttaac gaggaaagac tgcagtatgt gggctcaatg gtattaggac
     1981 taaatgatgc actggtggaa cttactggaa ccctggcagg ccttagcttt gcccttcaga
     2041 acacccgtat aatagcattg tccgggctta ttaccggaat ttccgctacc ctttcaatgg
     2101 cgtccagcga atttctatcg gcaaagagcg agggtaacaa agatgcctta aaatcgagtt
     2161 tctacacggg catcgcctat ctgataactg tagtaatgct gatattgcct tatctccttt
     2221 tgccgcccca aaaatacatg ctggccctgt ttacaatgct gattaccgta gtagcaatta
     2281 ttgccgcttt taattactat atagctgttg caaaggactt gccctttaaa cgaaggtttc
     2341 tcgaaatggc gacaatcagc ttaagtgttg cagtgctttc ctttgtggtg ggcctcatag
     2401 ttaaaaacgc actaggtgtt gacatttaat aacaggcact taaagccgaa tttaagtttt
     2461 gttttacgac tttaatctca gtgtctagca caggattggt agagttttac tggtgttttt
     2521 tatgatattt acgacggttg acaacgggat aaatcttgtg ctatactttt aaatagtcac
     2581 cggacaggag gggtgtccga gtggtttaag gagctggtct tgaaaatcag tgtctcgaaa
     2641 gagccgtggg ttcgaatccc accccctccg ccata
//
LOCUS       DAONUO010000282         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_26167_length_2658_cov_4.590088, whole genome shotgun sequence.
ACCESSION   DAONUO010000282 DAONUO010000000
VERSION     DAONUO010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26167_length_2658_cov_4.590088"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PK768_09160"
     CDS             <1..851
                     /locus_tag="PK768_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQA61178.1"
                     /translation="LMNMADAAIVSGRLQDAGFSVKRATELFGQLTGMAAPLVNLPVV
                     ATTALSASLVPAIAESMALNDREMIASRAETGMRLSIIFGLPAAVGMFVLAEPITVLL
                     YKNAEAGVSLELLSWGVVFLALTQTTTGILQGIGKTMIPVRNMLVGAVIKVVINYILT
                     GIPSINIKGAALGTVVGYLVPSLLNASQVVKWTNLKVNLNYMLFKPALAAAVMGAGVK
                     LVHGQFMLFGLSQNKATIFAIALGVIIYSLVLLLIGGLKQNDLRVLPGGNKLVLILQK
                     FGVFRR"
     gene            857..1645
                     /gene="mazG"
                     /locus_tag="PK768_09165"
     CDS             857..1645
                     /gene="mazG"
                     /locus_tag="PK768_09165"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQA61179.1"
                     /translation="MSRGKLEDLIEIMAKLRGNPGCPWDKAQTHESLKPYVIEEAYEI
                     VDAIDRNSTEDLIEELGDLLLQIVFHCRIAEERGVFDMSDVIEGVCEKMIRRHPHIFG
                     DLVVDGTEQVLRNWEEIKREEKDMKSESESMMNLPKALPALMKAFKVQEKAARIGFDW
                     DDVKGAVDKVYEELDELQEVYNAGNSDKIREEMGDLLFACVNVARFLDVEPELALNDA
                     VKKFITRFNFVEEAAAKSDKELKDMSLQEMEKLWQQSKNQEKKL"
     gene            1725..2000
                     /locus_tag="PK768_09170"
     CDS             1725..2000
                     /locus_tag="PK768_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQA61180.1"
                     /translation="MNKAELISVMAEKSGLTKKDSEKALNSFIEAVEEALVKGEKVQL
                     VGFGTFEVRERSARKGRNPQTGEEIDIPAASVPAFKAGKALRESINK"
     gene            2274..>2658
                     /locus_tag="PK768_09175"
     CDS             2274..>2658
                     /locus_tag="PK768_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HQA61181.1"
                     /translation="MYKKLIVGALVLTLFTAGMQGCARRAPEKKPAPPKIPEKISRGA
                     GKEPVLRVYEVQTHTVKEMKLEDYVAGVVAGEMENDWPVEALAAQAILARTYVLEFIT
                     DKGGSKYGDADISTDFEEAQAWNPDN"
BASE COUNT          772 a          524 c          720 g          642 t
ORIGIN      
        1 ccttgatgaa catggcggat gcggccattg tcagcggcag attgcaggat gccggttttt
       61 ccgtaaaaag ggcaacggag ttgttcggcc agctcaccgg catggcggca ccccttgtaa
      121 atttacccgt tgtggcaaca acggcccttt cagctagttt agtgccggcg attgccgagt
      181 ccatggcctt aaacgacagg gaaatgatcg cttcccgggc agagacgggc atgaggctca
      241 gcataatatt cggcctgccg gcggcagtgg gcatgtttgt cctggcagaa cccataacgg
      301 tcttgcttta caaaaatgcc gaggcaggtg tatcccttga gcttctatcc tggggcgtgg
      361 tctttttagc ccttacccag actaccacgg gaatccttca agggataggc aaaaccatga
      421 ttcccgtaag aaatatgctg gtgggcgccg ttataaaggt tgtaataaac tacatactca
      481 ccggaatacc ttccatcaac ataaagggcg ctgcccttgg aaccgtggtc ggttacctgg
      541 tgccttcgct cctaaatgct tcacaggttg tcaagtggac aaatctaaaa gtcaatttaa
      601 attacatgct atttaaacct gccctcgcag ctgctgtcat gggagcggga gtaaaacttg
      661 tccacgggca gttcatgcta ttcggcttgt cgcaaaataa ggccactatt tttgccatag
      721 cccttggtgt gatcatatat tctcttgtct tgctcttaat cgggggcttg aagcaaaatg
      781 atttgagagt tttacccggt ggaaacaagc tggtacttat cctacaaaaa tttggtgtct
      841 tcaggaggta atgtgattgt caagaggcaa gttggaggat ttaatagaaa taatggcaaa
      901 actcaggggt aatcccggct gtccttggga caaggcccag acccatgaaa gccttaaacc
      961 ttacgttatt gaagaagcct atgagatagt agatgccata gacagaaaca gcacggagga
     1021 tctcattgag gaactcgggg accttttgct gcagatagtc tttcactgcc gcatagccga
     1081 ggaacggggc gtgtttgaca tgagcgatgt catcgaagga gtttgcgaga agatgataag
     1141 gcgccatccc cacatcttcg gtgacctggt ggtggatggc accgagcagg tgttgagaaa
     1201 ctgggaagag ataaaacggg aagaaaaaga catgaaatcg gagtccgaga gcatgatgaa
     1261 cctgcccaaa gccttgccgg ccctgatgaa ggcttttaag gtgcaggaga aggccgcccg
     1321 gataggtttt gactgggatg acgtaaaggg ggccgtagat aaggtatatg aagagttgga
     1381 cgagctccaa gaggtatata atgcagggaa tagtgacaaa ataagagaag aaatgggaga
     1441 tttgctattt gcctgtgtga atgtagcccg gtttttggat gtcgaaccgg agctggcctt
     1501 aaatgatgct gtaaaaaaat ttataacgag gtttaatttt gtcgaggaag ctgcggccaa
     1561 aagcgacaag gaattaaagg atatgagctt acaggaaatg gaaaaactgt ggcagcaaag
     1621 taaaaatcaa gaaaaaaagt tgtaaatttc cttttaaaag aaggattttg tcaataagtg
     1681 gagaatagtc tatatgaatt aattttcatc agggagggaa tcttgtgaac aaagccgaac
     1741 ttatttctgt gatggctgaa aagagcggtc tgacaaaaaa ggattctgaa aaagcgttga
     1801 actccttcat cgaagctgta gaagaagctc tcgtaaaggg ggaaaaagtt cagttagttg
     1861 gttttggcac ctttgaagtg agagagagaa gcgctcgcaa aggtcgtaac cctcaaaccg
     1921 gcgaagaaat tgacatacct gcagccagtg ttccggcttt caaggcgggt aaagccctta
     1981 gggaaagcat taataaatag tgtgcaagat actactaaaa ggcaggtctt aaatggcctg
     2041 cttttgttct ttgttgatcc attgcccccg tgggggcttt tttattttaa aaacaagaat
     2101 tatttatcgg caaaaatcaa tactaagtat atatggtgag cccatttgca gtcaaaaccg
     2161 ttattagttt gcgggttcta attaaaaaac ctgtggaagt aaggggagga agcaaaggaa
     2221 ccgtcccctt gcttacaaaa aggtactaat tttgagtctc gaggaggatt tgcatgtaca
     2281 aaaaactaat tgtcggagca cttgttctca cgctttttac ggcaggtatg caaggatgtg
     2341 ccaggagggc gccggaaaag aaacctgccc cgcctaaaat accggaaaaa ataagtcgag
     2401 gggcaggcaa ggagccggtg cttagggttt acgaggttca gacccatacg gtaaaggaga
     2461 tgaagctgga agattacgtt gctggtgtgg tagcggggga aatggagaac gactggcccg
     2521 tggaagccct ggccgcacag gccatattag ccagaactta cgtcctggaa ttcataacgg
     2581 acaagggcgg ttcaaagtac ggtgatgctg acatatccac ggattttgaa gaggcccagg
     2641 cctggaatcc tgacaaca
//
LOCUS       DAONUO010000283         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_26386_length_2641_cov_3.595128, whole genome shotgun sequence.
ACCESSION   DAONUO010000283 DAONUO010000000
VERSION     DAONUO010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26386_length_2641_cov_3.595128"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK768_09180"
     CDS             complement(<1..453)
                     /locus_tag="PK768_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61182.1"
                     /translation="MSSFKKLTPTNGFDNNNLNNARQNNYAWSMSELGDYIYVGTSRN
                     MLINIISAISPNVRIPSLLMANPVENQGEIWRYRKDGKLPWQRVYRSTSTPGNSVLSG
                     FRYLITHRPFGGSPAIYAAEAGDRVRVLKSTNGVNWRVLEVTILQGSSS"
     gene            complement(549..>2641)
                     /locus_tag="PK768_09185"
     CDS             complement(549..>2641)
                     /locus_tag="PK768_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQA61183.1"
                     /translation="GEKLVALDIGPTGKLMKPQGDLSFEDAYRAFSEQIVAGEEAGAD
                     LILIETMSDVYEAKAAVMAAKENSKLPIICTMTFEQDGRSLTGTDVLTMVNILQNLGV
                     NALGANCSLGPKEMMQLVEKLVRYSKLPIIIQPNAGLPKIQGGKTIYETGPREFSRYA
                     RAMAEKGVTILGGCCGTTPEHIRALNDVLGDLKPIKRDVDDITAVSSYSKTVVLSEGI
                     NIIGERINPSGKPKMKEALKSCNYEYIIKEAIKQRDTGAKILNINVGLPEIDEEETMV
                     ELLTKLQSTINLPLMFDSPKPQVLEAACRIYSGRPIINSVNGKESSMREIFPIAKKYG
                     ACVIAMTLDEKGVPQTAEERLEKAEKIIERAKSYGIDKKDIIVDCLVLASSAEQEAAF
                     ETFRALRLIKERLGVLTILGISNISFGLPDRPLLNRTFLAAALGAGLDIPIIDPLDPE
                     MIKTVDAFNVLSGNDKNADYYISTYSKTVETEIASTERSNGYDLKFIISEGLTDEIKP
                     AIDELLDDNSPMEIVEGFLIPALDDVGKKFETGEIFLPQLIMSAEAAKTAFQIINSYN
                     KKIGSQSSFAKGKILLATVRGDIHEIGKNIVKAMLENYGYEVIDLGIDVPKEEIVARI
                     KQANIHLVGLSALMTNSLKSMEETIKAIRLIDTDCKVMVGGAVLNPEYANMIGADYYG
                     KDAREAVMIAQKVF"
BASE COUNT          645 a          614 c          527 g          855 t
ORIGIN      
        1 tgaggatgat ccttgaagaa tggtgacctc caaaacccgc caatttaccc cattggtaga
       61 ttttaatacc cgaactctat cgccggcttc cgctgcataa atagcggggc tgcctccgaa
      121 aggtctgtga gttatcaaat atctaaaacc tgacagcacg gagtttccag gagtgcttgt
      181 gctcctgtag actctttgcc aaggcaattt tccatctttt ctatatctcc atatttcacc
      241 ctgattttct acagggtttg ccatgagcag tgaaggaatc ctgacattgg gactaatagc
      301 agaaatgata tttatcagca tatttctact tgtgcccaca taaatgtaat ctcctaattc
      361 actcatggac catgcgtagt tgttttgtct tgcattattg aggttattat tatcaaaccc
      421 gtttgtcggg gttagcttct tgaaagaact cataaagtac atcctcctct tttttcaatg
      481 agactttttt cactataata tatgcccatg gtggttaaca ttgctactaa gagaaggatg
      541 tcttactttt aaaatacctt ttgcgctatc attacggctt cacgggcatc cttgccataa
      601 tagtcagcgc caatcatatt tgcatactca ggatttagga ctgcaccgcc caccattact
      661 ttacagtcgg tatcaattag tctaatggct ttaatggtct cctccatgct tttaagtgaa
      721 ttggtcatga gggcacttaa gccaacaaga tgaatatttg cctgttttat cctggcaaca
      781 atttcctctt tcggcacatc tatgcccaga tcaattactt catatccgta attttccagc
      841 atggctttca cgatattttt tcctatctcg tgaatatcac ccctgaccgt agctagcagt
      901 atcttgccct tagcaaatga actttggctt cctatttttt tattgtagga attaattatc
      961 tgaaaagcgg tcttggcggc ttcggcggac attatcagtt gcggcaggaa gatttcaccg
     1021 gtttcaaatt ttttcccgac atcgtcaagg gcgggaatta aaaagccttc aacgatttcc
     1081 atgggtgaat tatcgtctaa cagctcatct atagcaggtt ttatttcatc tgtcaatcct
     1141 tcacttatta taaactttaa atcatatccg ttagatcttt ccgtgctggc aatctctgtt
     1201 tcaacagttt ttgaataagt gctgatgtag tagtcggcat ttttgtcatt acctgacagc
     1261 acattaaagg catctaccgt ttttatcatt tcaggatcca gagggtcgat gattgggatg
     1321 tctaggcctg cacccagggc agctgccaaa aaggtcctgt ttaaaagtgg cctgtccggc
     1381 aaaccaaaag aaatgttgct gattccgaga atcgtcagga caccaagccg ttccttgatg
     1441 agccgcaggg ccctgaaggt ttcgaaagct gcttcttgct cggccgatga tgccagcacc
     1501 aggcagtcga cgataatatc ttttttatcg atgccgtagg attttgccct ttcaatgatt
     1561 ttttccgctt tttcgagcct ttcttcagca gtttgtggga cacctttttc atctaaggtc
     1621 atggcaatta cacaggctcc gtattttttg gctataggga agatttccct catggaactt
     1681 tctttgccgt ttaccgagtt tattatcggt cttccactat atatcctgca ggccgcttcc
     1741 aacacttggg gttttgggct gtcaaacata aggggcagat taatggtact ttggagtttt
     1801 gtcaatagct caaccatggt ctcttcttcg tcaatttccg gaaggcctac attaatattt
     1861 aatattttag cccccgtatc cctttgttta atagcttcct taattatgta ttcatagttg
     1921 cagcttttaa gggcttcctt catcttgggc tttcccgaag ggtttatcct ctcaccaata
     1981 atattgatgc cctctgacaa aacaacggtt ttggaataag aacttacggc agtaatatca
     2041 tcgacatctc gttttatggg ctttaaatcg ccgagcacat cattaagcgc cctaatatgt
     2101 tcaggagttg tgccgcagca tccacctagt attgtaacgc ccttttccgc catagctctg
     2161 gcgtaacgag aaaactcccg gggaccagtt tcgtaaatgg tttttccgcc ttggattttg
     2221 ggaaggcctg catttggctg aatgattatc ggtagcttgg aataccgaac caatttttca
     2281 accaactgca tcatttcctt tggccccaat gagcagttgg cacctaaggc attgacccct
     2341 aggttctgaa ggatatttac catggtaaga acgtctgttc cggttaaaga ccttccgtcc
     2401 tgttcgaatg tcatagtgca tatgattgga agcttgctgt tttctttagc ggccataacg
     2461 gcagctttag cttcataaac atccgacatc gtctcaatga gaattaaatc tgctccggcc
     2521 tcttcgcccg ccacaatttg ctcggaaaag gccctgtatg catcttcaaa ggataaatca
     2581 ccctggggct tcatgagctt tccggtaggt cctatatcca gggcaaccag tttttccccg
     2641 g
//
LOCUS       DAONUO010000284         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_26850_length_2605_cov_3.618824, whole genome shotgun sequence.
ACCESSION   DAONUO010000284 DAONUO010000000
VERSION     DAONUO010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26850_length_2605_cov_3.618824"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PK768_09190"
     CDS             complement(<1..610)
                     /locus_tag="PK768_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQA61184.1"
                     /translation="MKYDAAVIGAGPAGAYCAKILASRGLKVLLMDKEKFPRDKVCGG
                     LISAKALELIQDDQLNKLLSKIRPNPVQKIILTCGQKEVTKKKDRVLGLVVKRKEFDE
                     ALVNIAADGGADFVDHCEYITHRDLKSVFEIYTTKGTFFSDYLIGADGAFSKVARESK
                     LRQRFLKWEMGFAVCSEIPREMIIEKNGIEFVLAKVLGGMGWV"
     gene            complement(742..2316)
                     /locus_tag="PK768_09195"
     CDS             complement(742..2316)
                     /locus_tag="PK768_09195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA61185.1"
                     /translation="MKISFYGACRTVTGSCCLIETNDTKLLIDCGMFQGSKDLKELNY
                     GEFEFAPSEIDYVILTHAHIDHSGLIPKLVKKGFKGTIIATKPTTDLCSIMLPDSGHI
                     QELEVERKNRKLARAGKPLIEPIYTHIDAQRALMQFSTLDYGLKVDLTPTVSIRFQDA
                     GHILGSTIVEMWVKENKVETKIVFSGDIGNLNQPIVHDPTVIQEADYLVMETTYGNRL
                     HKNIGDKQELLLQIIESTFLRGGNVIIPAFAVERTQDLLYYLSRLDLEGRLPDCKIFV
                     DSPLAISANEIFKVSVKYFDDETKAAFGKIWESPGILKRIVMTRSAEESKKLNEIKTN
                     AIIISASGMCDAGRIKHHLKHNLWREESSVVFVGYQAPGTLGRQIVDGKKQVRIHGEQ
                     IAVNAQIHNLDGFSGHADQEALLQWFDSFKQKPKEVFLVHGEPDGMEAFKNLLLERYD
                     VEVHMPELNQTFDLKHGQVKTYDLAGPTEKDLAYIYANIINKLSELYKVNIDRQEVKQ
                     LYDKLKAIERAIEDVH"
BASE COUNT          747 a          573 c          408 g          877 t
ORIGIN      
        1 aaacccagcc catgccacct aagacctttg ctaacacaaa ttcaattcca tttttttcaa
       61 taatcatttc cctaggaatt tcactgcata ctgcaaaacc catttcccac tttaaaaagc
      121 gctgtctcaa ctttgattct ctagcgactt tactaaatgc tccgtctgcc ccaatcagat
      181 aatcgctgaa aaacgttccc ttcgtcgtat aaatctcaaa aacgcttttt aaatctctgt
      241 gcgttatata ttcacagtgg tccacaaaat ctgcccctcc atccgcagca atgttcacca
      301 aagcttcatc gaattctttc ctttttacca caaggcctaa tacacggtct ttctttttag
      361 tcacttcctt ttgaccgcat gttaaaatga ttttttggac aggattaggc cttattttac
      421 ttaaaagttt attaagttga tcatcttgaa taagctctaa ggccttagct gatataaggc
      481 ccccgcacac tttatcccga ggaaactttt ctttatccat caaaagaacc ttcaaacctc
      541 ttgatgcaag gatcttcgca caatacgctc ccgcaggccc cgcaccgatt actgcagcat
      601 cgtatttcat gtcatactcc tcaaattctt agtgtttatt tatcttgccc aaaagaaata
      661 attctttaca caaaaaaaac agagtagaaa caatatctac tctgaaaatc ctgatttttg
      721 tggatagata ttcagatagc tttaatgaac atcttctatt gctctttcga tggctttcaa
      781 tttatcataa agctgtttta cctcttgacg gtcaatattt actttatata gttcgctcag
      841 tttgtttatg atatttgcat aaatataggc taaatccttt tctgtaggtc ctgcaagatc
      901 gtaagttttt acttggccat gcttcaaatc gaaagtctga ttaagctcgg gcatatgtac
      961 ttctacatcg tacctttcca gtaaaagatt cttaaatgcc tccattccat caggctcgcc
     1021 atggaccaaa aaaacttctt tgggtttttg tttaaaggaa tcaaaccatt ggagtaaagc
     1081 ttcctgatct gcatggcctg aaaatccatc taaattatgt atctgggcat ttactgcaat
     1141 ttgttctcca tgtattctca cttgtttttt cccgtccaca atttgtcgtc cgagagttcc
     1201 tggggcttga tatcctacga aaacaacaga ggattcttcc cgccacaaat tgtgttttaa
     1261 atgatgcttt attctgcccg catcacacat gccgctggca gatataatta tggcattagt
     1321 cttgatttca tttagctttt ttgattcttc agcggaacga gtcatgacta tcctttttaa
     1381 aattccgggg gactcccata ttttgccgaa agcagccttt gtttcatcgt cgaaatactt
     1441 tacactcacc ttgaaaattt catttgctga tatagctaag ggactgtcaa caaatatctt
     1501 gcaatccggt aaacgtccct ctaaatccag tcggctaaga taatacaaca agtcctgggt
     1561 cctctctacg gcaaaggcag gtattatgac attgcctccc cttaaaaaag tcgattcgat
     1621 tatttgcagc aggagctcct gtttatctcc aatattctta tgtaacctat tgccataggt
     1681 ggtttccatg actaaataat ctgcttcttg aattacggtg ggatcatgta ctataggttg
     1741 attcaaatta cctatatctc cgctaaagac aattttagtt tcaaccttat tctcctttac
     1801 ccacatttca acaatggtag aacctaatat gtggcccgca tcctgaaatc ttattgaaac
     1861 tgtaggagtt aaatccactt ttaaaccata atctaaagtt gaaaattgca ttaaggctcg
     1921 ttgcgcatct atatgtgtgt aaatgggctc gattaaaggt tttcctgccc tcgcaagttt
     1981 cctattcttt ctttccactt ctaattcctg aatatgccca ctatcaggca acataatgga
     2041 gcacaaatcg gttgtcggtt ttgtagcgat aatagtccct ttaaatcctt tctttacaag
     2101 tttcggtatt aatccactgt ggtctatgtg ggcatgggtg agaataacat agtcaatttc
     2161 gcttggtgca aactcaaact ctccgtagtt taattctttt aagtccttag agccttgaaa
     2221 cataccacaa tcaatcagta gttttgtgtc attagtttct attaaacaac aagaaccggt
     2281 tacagttcta catgctccgt aaaaagatat tttcaacaag accccgacct ttcaatacat
     2341 gtatatattt ttataagtga aataagcttt taattgttac gttatagacc gtagaagata
     2401 tacaatttta gatagagatt tttactgtgt taggacattt tttctttaat agcaatattc
     2461 tatatttatt ataacacaaa agggtccgtt gggctatgca gcacaaaaaa agggctttta
     2521 aattaagccc ctttagaacc aagatcaaat attttgtaca taaaaaatgg ctcctcgagc
     2581 aggattcgaa cctgcgaccc ttcgg
//
LOCUS       DAONUO010000285         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_27018_length_2591_cov_3.460568, whole genome shotgun sequence.
ACCESSION   DAONUO010000285 DAONUO010000000
VERSION     DAONUO010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27018_length_2591_cov_3.460568"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1357)
                     /locus_tag="PK768_09200"
     CDS             complement(206..1357)
                     /locus_tag="PK768_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQA61186.1"
                     /translation="MKSKIYFADARASGYDFKYSFIAKFEQILDMIDFKEFISEKDYV
                     AIKTHFGSYGGHRIVRPIFLKKVVDKVKEVGGRPFVTDTVRIPGLEYLEVANMEGINH
                     LSVGAPVILADGIFGRDSIMVKSGPILNEIGVASAIYHAPAMIVVSHCKGHIGSGFGG
                     AIKNLGMGGISCKNHCGEAERGRIHFEENKHLEWQDAVCIRCGQCVDVCPHDAIELID
                     DSIVIDKKKCVKCARCSRVCEVKALLVPISEESFQKGLAESAYAVVSTFEKGRILYIN
                     FVTEVQPECDCMPMSDTPLVQDQGVLVSFDPVAADQAALDLINNATPLPQSKAEDKNV
                     KKGDNILKAVTGKNAQLHLDAAYELGMGSKEYELITITEKDIKEEDEEE"
     gene            complement(1620..2471)
                     /gene="yycI"
                     /locus_tag="PK768_09205"
     CDS             complement(1620..2471)
                     /gene="yycI"
                     /locus_tag="PK768_09205"
                     /inference="COORDINATES: protein motif:HMM:NF021186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HQA61187.1"
                     /translation="MQWKQAITILIVSFIVLNIVMILNLWLRDRPGDQFALSSSQKSE
                     IIESLRQKGVIVEVEIPKEGKPQALLEVALPKLDEKKILQNFFGKDSRPILSHTQDGR
                     KYTHGNEQLIITDHGFITYFNSDDSVIWPNLSREQAEKEALAFMKARRSMQENAILDR
                     VTYDDESKAYLLEYVSYHDGFFIQNSYATVLVTPSGIKGYYQCWLEPLGYVGKKRSVL
                     SPLTAILRVINERSTAEPIIITGIQQGYYSKLYDADRWQVAPVWKIQLGNKETYYVNA
                     YTGELEQ"
BASE COUNT          641 a          674 c          504 g          772 t
ORIGIN      
        1 cgtcactttc tgcctgtccg aggcgcgcct ggggctgctg cctgccatac atgagcgatg
       61 aagtaataaa ggtagctgcc agtgccccaa tgagtgcact gaatacagct accaccagca
      121 tcttcttcat ttccggccag gaaatcttat tattctgcat ttgctgcacc taccctaaaa
      181 gctttagccc tgaccggaaa aagtactatt cttcttcgtc ctcttctttt atatcctttt
      241 ccgtaatcgt aatgagctca tattccttgc tacccattcc cagttcatag gctgcatcaa
      301 ggtgaagctg ggcatttttg ccggtcacgg cctttaatat attatcaccc tttttcacat
      361 ttttatcctc ggctttcgac tggggcaggg gtgtggcatt atttatcaaa tccagggcag
      421 cttggtcggc agctacaggg tcaaaggaaa cgaggacacc ctgatcctgt accagcggtg
      481 tgtctgacat gggcatacag tcacattcag gctgcacttc ggtgacaaag tttatgtaaa
      541 gaatcctgcc cttttcaaag gtggacacta ccgcatatgc cgattcagca aggccctttt
      601 gaaaactctc ttccgatatg ggcacaagca gtgcctttac ttcgcaaacc cgggagcagc
      661 gtgcacattt tacgcacttt ttcttgtcta taacgatact gtcatctatg agctctatgg
      721 catcatgggg gcatacatcc acgcactggc cgcagcgaat gcatacggca tcctgccact
      781 ctaggtgttt gttctcttca aaatgtatcc ttcccctttc agcttcaccg caatggtttt
      841 tgcagcttat gcctcccatg cccaagttct taatggcacc gccaaagcct gaccctatgt
      901 ggcccttgca gtgagacact acaatcatgg caggtgcatg gtatatggcg gaagcaactc
      961 caatctcatt taaaatgggg ccgctcttta ccattatact atccctgccg aagattccgt
     1021 cggccagaat aacgggagcg ccaaccgaca gatggtttat tccctccata tttgccactt
     1081 ctaaatactc aaggccgggg attctcacag tatcggtcac aaaaggcctg ccgcccacct
     1141 cctttacctt gtctaccact ttctttaaaa agatgggacg aacgatgcga tggccaccat
     1201 aggaaccgaa atgggtcttg atggcaacat agtctttttc agagatgaat tccttaaaat
     1261 caatcatgtc gaggatttgt tcaaatttgg ctataaagct gtacttgaaa tcatacccgc
     1321 ttgctcttgc atcggcgaaa tagatttttg acttcatatt caacctccta cgcaattttg
     1381 ctaatctata tagttttgaa tgtgttataa cgtggatcgc tcgctttaac tatattccac
     1441 gcaaaatcct tttttccttc taggccttat cgacattatt ttagtatttt ctatttcccg
     1501 ggaaataatt cctcaaaaga aattgtccaa aaactcctcc cagtagcccg gccccctgct
     1561 tgctgtcgtg tcccaaaaac atcactatta cacaacaaaa gcggcagaat gccgcaaaat
     1621 cactgttcca attccccggt ataagcattt acatagtagg tttctttatt tcccaactga
     1681 attttccata ccggagctac ctgccatctg tccgcatcgt acagcttgga ataatagccc
     1741 tgctgaattc ccgttattat aatgggctct gccgtactcc tttcattgat aactctcaaa
     1801 attgccgtaa ggggtgaaag cacgcttcgc ttcttgccca catagcctaa aggctccagc
     1861 cagcactgat aatagccctt tattcctgaa ggggtaacaa ggacagtggc atagctattt
     1921 tgtataaaaa agccatcgtg ataactcacg tattccaaca aataggcctt gctttcatcg
     1981 tcataggtaa ccctatccag aatcgcattt tcctgcatgc tccgcctggc tttcataaag
     2041 gccagtgcct ctttttcggc ctgctccctc gataaatttg gccatattac actgtcgtca
     2101 ctgttaaaat acgttatgaa gccatggtca gttatgatta gttgctcatt gccatgggtg
     2161 tatttcctgc cgtcttgagt gtgagagagg attggcctgc tgtccttgcc aaaaaaattt
     2221 tgtagtatct ttttttcatc aagttttggc aaggctacct ccaaaagcgc ctgaggcttg
     2281 ccttctttgg ggatctccac ctcgactatt acgccctttt gcctcagact ctcaattatt
     2341 tcagattttt gacttgaaga gagggcaaac tggtcgcctg gccggtccct taaccacagg
     2401 tttagtatca ttacgatatt gagcactata aaagatacta ttagaatagt aattgcttgc
     2461 ttccactgca aaggcatcac tccgaaaggc tgtgtatgat cacttccatc ccgcttgttt
     2521 ctaccaccca gacaggtttg agttcttcat cttcaactac atatgccgga tgcacatcac
     2581 ttacaacttt t
//
LOCUS       DAONUO010000286         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_27083_length_2586_cov_4.197155, whole genome shotgun sequence.
ACCESSION   DAONUO010000286 DAONUO010000000
VERSION     DAONUO010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27083_length_2586_cov_4.197155"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PK768_09210"
     CDS             complement(<1..1418)
                     /locus_tag="PK768_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HQA61188.1"
                     /translation="MEKIIKQLANELNIREKQVAATVALLDEGNTVPFIARYRKEVTG
                     GLSDEQLRALSERLGYLRNLEDRKAEVKRLLEEMEKLTPDIEQSLDEAITLQEVEDIY
                     RPFRPKRRTRATVAREKGLEPLAQKILAQEAPVTEEDAAAFIDPEKAVETADDALAGA
                     LDIIAEIVSDDAEIRKLIRDMAYEKGIVETTGLTEETSTYSMYYDFKEPVARIVSHRV
                     LAINRGEREKFLQVKIQVPEEDLLKAIKAKYIKNPSPTSELMERAVDDAYKRLIWPSI
                     EREIRNILTEKAEEQALVTFSKNLKHLLLQAPAKDKAILGFDPAYRTGCKLAVIDSSG
                     RLLDHAVCYPTPPQNAFEESKKLILALIERHNVDVISLGNGTASRESERFLAEILKDA
                     KRPVSYVVVSEAGASVYSASKLAAEEFPDLDVSFRGAVSIARRLQDPLAELVKIDPKA
                     LGVGQYQHDVNQKKLSEKLAGVV"
     gene            1691..2347
                     /locus_tag="PK768_09215"
     CDS             1691..2347
                     /locus_tag="PK768_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing C40
                     family peptidase"
                     /protein_id="HQA61189.1"
                     /translation="MVNRAKVLMSLIAMAVMFCMLPGISYAANYHVVQPKETLWGISR
                     LYGLTVDELFRLNNLQSDLIHPGQRLLVSKDEAEGENFDESHAATEEAAEEEKDLATQ
                     IIDFAKTFIGTPYKSAGSSPGGFDCSGFTSYVYKQFDIDLPRVAQDQYHFGKAVSSEE
                     AKAGDLVAFAHNGYIHHVGIYMGNGEFIHSSSTEGIVITKTSDIYWGPRLLGYCRVLE
                     "
BASE COUNT          619 a          585 c          655 g          727 t
ORIGIN      
        1 acgactccgg ccagcttttc cgagagtttt ttttggttta cgtcgtgctg gtactgtccc
       61 acaccaaggg ctttcgggtc aatttttacc agctctgcca aagggtcctg gaggcgccgg
      121 gcgatggaaa cggcaccacg gaaggacacg tccaggtcgg gaaattcttc ggcggccagc
      181 tttgatgcgg agtagacgga agcccccgct tcgctcacca ctacgtagga tacgggcctt
      241 tttgcgtctt ttaatatttc agccaggaag cgttcgctct ctcgagaggc agtgccgttg
      301 ccaagggata tgacatccac attgtgcctt tctatcaaag cgagaattag cttttttgac
      361 tcctcaaaag cgttttgcgg cggagtgggg tagcagacgg catggtctag cagtcttccc
      421 gagctgtcga taaccgccag cttgcagccg gtccggtagg ccgggtcaaa gcctagaatt
      481 gccttgtctt ttgccggggc ctgcaggagc aggtgcttta gattctttga aaaggtcaca
      541 agggcctgct cttctgcctt ttccgtcaat atgtttcgaa tttcccgttc aatggagggc
      601 caaatgaggc gcttgtaggc atcatctact gccctttcca tcagctcgga tgtgggcgac
      661 ggatttttta tatacttggc cttaatggcc tttaataaat cttcctcggg cacctggatc
      721 ttcacctgca ggaatttttc ccgctcgccc cgatttatgg cgaggacccg gtgggagacg
      781 atccttgcca ccggttcttt aaaatcgtag tacatggagt aagtggacgt ctcttccgta
      841 aggcctgttg tttcaacgat gcctttctca taggccatgt cccggatgag ctttcgtatt
      901 tcggcatcat cggatacaat ctcggcaatg atgtcaaggg cgccagctaa ggcatcatcg
      961 gcagtttcaa cagctttttc tgggtcaatg aaggccgctg catcttcctc ggtaaccggg
     1021 gcttcctggg ccaggatttt ctgggccaag ggctccaggc ccttttccct ggccacggtg
     1081 gccctggtcc ttcgcttggg cctgaagggc cggtagatat cttccacttc ctgtagggtt
     1141 atggcttcat ccagactttg ttcaatatcc ggcgtgagct tttccatctc ctccaggagc
     1201 cttttaactt ccgcctttct gtcttcaagg tttctgaggt agccgagccg ctctgaaagg
     1261 gcccttagct gttcatcgga aagtcccccg gtgacctcct tccggtagcg ggctataaag
     1321 gggacggtat ttccctcatc aagtaaggcg acggttgcag ccacctgttt ttcgcggata
     1381 tttaattcat tagctaattg ttttatgatt ttttccatat tttcctccca aatagcttcc
     1441 taatcataat gtaacaccta tacaatattt tatatttgct gcgaaatttc ctgcaaagct
     1501 cataactaat ttaaggtcgt tagagttttt gaaatgttag agttttcaag aaaaagtgca
     1561 gaaattcgtc atttttagct tcaagtttgg gagattgaaa ctagataaag ttgactagtt
     1621 gaggggcagc ctatatactt caagtataaa atgagacaag cagattttca gttgcggaaa
     1681 gtggggtact atggtaaata gagccaaagt attaatgtcg cttattgcaa tggcagttat
     1741 gttttgcatg ttacctggta tttcctatgc agcaaactac catgttgttc agccgaaaga
     1801 gaccctgtgg ggaatatctc gactgtacgg tcttaccgtg gacgaactat ttcgattgaa
     1861 caaccttcaa agtgacctga tacacccggg ccagaggctg cttgtcagca aagatgaggc
     1921 tgaaggggaa aacttcgatg aaagccatgc agctacagaa gaggcagcgg aggaagagaa
     1981 agacctagcc acgcaaatta tcgactttgc caagactttt atcggcacac cgtataagag
     2041 tgcaggctca tcgccgggtg ggtttgactg ttcgggtttt accagttacg tgtacaagca
     2101 gttcgacatt gatttaccgc gagtggcaca agaccaatac cattttggaa aagccgtaag
     2161 cagcgaagaa gccaaagcgg gggatttggt agcctttgct cacaacgggt acatacacca
     2221 tgtgggaatc tacatgggca acggtgagtt tatacatagc agctctaccg agggtattgt
     2281 gattacaaag accagcgata tatattgggg tccccgattg ctgggttact gcagggttct
     2341 tgaataaccg gataattgat ttacaaacac ctctgattaa ccgtagaggt gttttttttt
     2401 cgataattac gagtattata acattgaaat tgcatggcac ccgtgatatt attcagataa
     2461 ggaaattgtg aagcagataa cgattgtacc ggagaaacaa tcgcgagttt gtgaatataa
     2521 tctattaaag gcctatggtt tttgctttgc cgagggaatg gacataagtg ggccggtgag
     2581 gagatg
//
LOCUS       DAONUO010000287         2582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_27126_length_2582_cov_3.025326, whole genome shotgun sequence.
ACCESSION   DAONUO010000287 DAONUO010000000
VERSION     DAONUO010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27126_length_2582_cov_3.025326"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK768_09220"
     CDS             <1..181
                     /locus_tag="PK768_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phenolic acid decarboxylase subunit B"
                     /protein_id="HQA61190.1"
                     /translation="PIHLENMLKLSRIGVVIMPPVPAFYNKPKSIDEIVNHTIARVLD
                     HLRIDNDLTKRWGNT"
     gene            258..1667
                     /locus_tag="PK768_09225"
     CDS             258..1667
                     /locus_tag="PK768_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQA61191.1"
                     /translation="MKSMSADDKHRMMINTPVNELILKLAVPSIVSMLISSIYNMADT
                     FFVSKISTSAAGGVGISFSLMAMIQAIGFTLGMGSGNNISRLLGQKEKEKAAKVAATG
                     FFTALALGAVLGILGLVFLEPFVYALGATTTIAPHAKNYIRYILIGGPYMTASLVLNN
                     ILRYQGSAYLGMMGIATGAIINIVLDPIFIFGLNLGTAGAAIATIISQFISFCILYHN
                     SSKGGNVKIQFENFTPNWGVYKQILKGGMPSFYRQSLGSIAMICLNNSASVYGDAAVA
                     AMAIVTRIFQFTTMIMHGFSQGYQPVCGFNYGAKRYDRVLEGFRFCVKVSAVFLTLVS
                     AVGFVFSLQIISTFRKEDLEVVAIGTRALKFYCLAFPLASWTIMTDIILQTLGKAAKG
                     SIVAASRQGLFFLPAILILPKKYGILGVQISQAISDVCSFLIALPIALSVINELKSKQ
                     IEIEAAEATSTRKASLDMV"
     gene            2173..2496
                     /locus_tag="PK768_09230"
     CDS             2173..2496
                     /locus_tag="PK768_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61192.1"
                     /translation="MKEALEDVKAAEKQARDILEEAEKEAKAIIAEASQKGETILQDA
                     RRQGEVEGQKLVEQAEADAVKMAEDLKKDCEEQVKHLREVSAGKMDTAVNMILERIVK
                     VHGNS"
BASE COUNT          822 a          466 c          579 g          715 t
ORIGIN      
        1 ccccattcac cttgaaaata tgctgaaact atccaggatt ggtgttgtaa taatgccgcc
       61 ggtgccggct ttttataaca aaccaaaaag catagacgaa atcgtaaatc atacaatagc
      121 acgggtttta gatcacctca ggatagacaa cgatttaacc aaacgctggg ggaataccta
      181 gagcacaaac aggaaaagaa caagaatttg tcgggaaaat ctgcatattt aataccattg
      241 ggaaatgagt ggtagaaatg aagagcatga gtgctgatga caaacaccgc atgatgataa
      301 acactcccgt aaatgagctg attttaaaac ttgcagttcc ttctatcgta agtatgctaa
      361 ttagttcaat ctacaatatg gctgacacat tttttgtgag taaaataagt actagtgctg
      421 caggtggtgt agggatttcc ttctccctca tggcaatgat ccaagccatt ggttttaccc
      481 taggtatggg ttccggaaac aatatctcta gattattagg gcaaaaagaa aaagaaaagg
      541 ctgcaaaagt tgcggccact ggatttttta cagccctggc cctaggtgca gttttaggca
      601 tactcggcct tgttttttta gagccttttg tatacgcctt aggagcaact accacgattg
      661 ccccccacgc aaaaaattac atcaggtaca tattgatagg cggcccctac atgactgcat
      721 ctctcgttct aaacaatatt ctccgctatc agggcagcgc ttacttgggc atgatgggca
      781 tagcaactgg agctattatc aacattgtcc tggaccccat tttcatattt ggtctgaact
      841 tgggcactgc aggcgcagca atagctacga ttataagcca gtttataagt ttttgtatct
      901 tatatcataa ttcttctaaa ggcggaaatg taaaaatcca atttgagaat tttactccaa
      961 attggggagt ttataaacaa atccttaagg gcggcatgcc gtctttttat cgtcagtccc
     1021 tcggcagtat agctatgatt tgcttgaata atagtgccag tgtttacggc gatgctgccg
     1081 tggctgcaat ggctattgtg acaaggattt ttcagtttac gactatgata atgcatggtt
     1141 ttagccaggg ttaccaaccg gtttgtggct ttaattacgg agccaaacgt tacgacaggg
     1201 ttcttgaggg attcaggttt tgcgtaaaag tttcggctgt atttttgacc ttagtaagtg
     1261 cagtcggctt tgttttttct ctgcaaataa tatctacatt taggaaagag gatttggaag
     1321 tagttgcgat agggacgcga gcgctcaaat tttattgcct tgcttttccc ctagcatcgt
     1381 ggaccataat gaccgatata atattgcaga ctttaggaaa agcagcaaaa ggatcgattg
     1441 ttgctgcatc aaggcagggc ttatttttct tacctgcaat cctcattctt ccgaaaaaat
     1501 atggaattct tggtgtgcaa ataagccagg ccatatccga tgtttgctct ttcttaatag
     1561 ccttacctat agccttaagc gttataaacg aattaaagtc aaaacaaatt gagattgagg
     1621 cagcagaggc aacgtcaact cgaaaagctt cattagatat ggtgtaatgg ctgtcagtat
     1681 ataagaagga aatcataaag acatatgagc ctgaaatgat agaaaaagtg aagattgtga
     1741 gccaagtctt tgggacaaaa acagcaggaa tgcccaggaa aacggaaaaa aggacttgcg
     1801 aatgtgattc ttttcacata taataaaaat gtaaattgac atatatataa caaaacccca
     1861 atataaaata tagtttgaat aatttttatc agtattgtat aatttatacc atgaaagaat
     1921 ttaagtactt acactacatt gcccttgttt ttaaaatagg aataagtgtg gcactaccaa
     1981 tagtaggtgg tatatatttt ggagcccatt tggacaaaca attatccact ggtagcgcct
     2041 ttttaattgc ctgcgcatta ataggaattt tcaccggagc ttatagtgct tataagttgc
     2101 tgtctaaggt tataaataac aataataaaa gaacttaaaa tttgctccaa tctacggggg
     2161 aggtggtagc tgttgaaaga agcattggaa gatgttaaag ctgctgagaa gcaagccaga
     2221 gacatcttgg aagaagccga aaaagaggcg aaagccataa tagcggaggc ttcccagaag
     2281 ggcgaaacta tccttcagga tgccagaagg caaggtgaag tggaaggcca aaaactcgtt
     2341 gagcaggcag aagccgatgc tgttaaaatg gcggaggact tgaagaagga ctgtgaagag
     2401 caggtcaaac atctaagaga ggtttccgct ggcaagatgg atacggcggt aaacatgatt
     2461 ttggagagga ttgtgaaggt ccatggcaat agttaacatg aagaagatgc acctcctggg
     2521 cctaaaacta gaaaaagcca caatactgag agctttgcaa aaaaccgggg ttgtggaagt
     2581 aa
//
LOCUS       DAONUO010000288         2571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_27290_length_2571_cov_4.402623, whole genome shotgun sequence.
ACCESSION   DAONUO010000288 DAONUO010000000
VERSION     DAONUO010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27290_length_2571_cov_4.402623"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PK768_09235"
     CDS             <1..570
                     /locus_tag="PK768_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HQA61193.1"
                     /translation="VRKFYGLEGLMNASLNVLFPLVLALGKILHIPKDVIKSSYIEVN
                     NKLLQSRSYSIKPGDILILAPHCLQKWDCPHKITADASNCRHCGRCNIDKLLKMSNDY
                     GVHLAVVTGGTLARKKVIDTKPKAIIAIACERDLTEGILDTNPLPVMGVINIRPEGPC
                     KNTQVDLDKVTEAIDFFINGKESTSIEKS"
     gene            557..1903
                     /gene="rsmB"
                     /locus_tag="PK768_09240"
     CDS             557..1903
                     /gene="rsmB"
                     /locus_tag="PK768_09240"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQA61194.1"
                     /translation="MKSPREIAVSILVKTQAGSYSNLLLSQYLNDNITQKDRALITQL
                     VNGVIQNKLLLDYIMSQFSKIKLSKLTPFVKNAIRLGIYQVYFLNRVPDFAAVNESVN
                     LVKKNEGRKAANFTNAVLRNVLRHKEMLSFPEKDKDITKYLSVYYSFPPWMVKRWLGL
                     FGADFTEKLCRAFNEPPCLCIRVNTLLTNRNNLLQRLKDEGVHAVPGKFADEAIYILQ
                     SPPIHQLKSFEDGLFTVQDESSIIASLAVDPKANEQVLDVAAAPGGKSTHMAALMQNQ
                     GTIVSWDIHPHRVKLIEENASRLKASIVKTRVKDAKILDETMMGSFDRVLIDAPCSGL
                     GVIRRKPDIKWSKKPDDIKALRNEQEHIIFVCSKYVKPGGVLVYSTCSIEPKENEELV
                     EDFLAKNQEFAYDDLRKSLPEMLHDELKSPYGYITLYPHIHGTDGFFVARLKRLKT"
     gene            1915..>2571
                     /gene="rlmN"
                     /locus_tag="PK768_09245"
     CDS             1915..>2571
                     /gene="rlmN"
                     /locus_tag="PK768_09245"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQA61195.1"
                     /translation="MSKTNLKSLTLNQLEEFMKQIAEPTYRAKQVFKWIYKGETQFEK
                     MTDLPRNLIKKLNERAYISIIGKYKKHESTLDGTIKYVFLLEDGNMVESVKMMYSYGI
                     TACISSQVGCPMGCTFCASAEGGMVRNLTWWEMADEILAIERDSGDKVSRVVVMGSGE
                     PLLNYDELIRFLNVLHNPNAFNMSFRRITVSTCGIVPEIYRLADEGIPITLAVSLHAP
                     N"
BASE COUNT          836 a          458 c          573 g          704 t
ORIGIN      
        1 gttaggaaat tttacggctt ggaaggttta atgaatgctt cccttaacgt actgtttcca
       61 ttagttttag ccttgggtaa aatcctccat atacccaagg acgtaatcaa gagctcctat
      121 attgaagtaa acaacaaatt acttcaaagc cgtagctata gtattaaacc gggggatata
      181 cttattctgg cgcctcactg cctgcagaaa tgggattgtc cgcataaaat tactgccgat
      241 gcatccaatt gccggcactg cgggcgatgc aacatagata aattgttaaa gatgagcaat
      301 gactacggcg ttcatttagc agtagtaact ggtgggacct tggctaggaa aaaagtaatt
      361 gacaccaaac ctaaagccat aattgcaatc gcctgtgaaa gggacttgac tgaaggcata
      421 ttagatacga accctttgcc ggtgatggga gttattaaca ttaggcctga ggggccctgt
      481 aaaaacaccc aggtagattt ggataaggtc acggaagcca ttgacttttt tataaatggg
      541 aaggagtcaa catcaattga aaagtcctag ggaaatcgct gtatcaatac tggtaaagac
      601 ccaggctgga tcatacagca atttactttt aagtcagtat ctaaatgata atattacaca
      661 aaaagatagg gcactaatta cccaattagt caatggagtt atacagaaca aactgttact
      721 ggattatata atgtcacaat tttccaaaat aaaattatct aagttgactc cctttgtaaa
      781 aaacgccata agactgggga tatatcaggt ttattttctt aaccgggtgc cggattttgc
      841 agcagtcaac gagtcggtaa accttgttaa aaaaaatgag ggtcgaaaag ctgctaattt
      901 taccaatgct gtgctgagaa atgtacttag gcataaagag atgttatcct ttcctgaaaa
      961 agacaaggat ataacgaagt atttatctgt ttactattca ttcccacctt ggatggtaaa
     1021 gagatggctt ggtctgttcg gtgctgattt tacagaaaag ctgtgtaggg cttttaatga
     1081 gccgccgtgc ttgtgtatca gagtaaacac tctcttgaca aacaggaata atttattgca
     1141 acgattaaag gatgaaggtg tccatgccgt ccccggtaaa tttgcagatg aagctatata
     1201 tatattacag tcacctccta ttcatcagtt aaaaagcttt gaggatggcc tttttaccgt
     1261 gcaggatgaa agttccataa ttgcctcctt ggcggtagat cctaaagcta atgaacaggt
     1321 tctcgatgtg gctgctgccc ctggtggtaa aagcactcac atggctgccc ttatgcaaaa
     1381 tcaagggaca attgtatcat gggatattca tccccacaga gtaaaactta tagaagagaa
     1441 tgccagtcga ttaaaagcca gcattgtgaa aacacgagta aaagacgcaa aaattctcga
     1501 cgagacaatg atgggtagct tcgatagggt cttgatagat gctccctgtt ccggactcgg
     1561 agttatccgc agaaaacctg atataaaatg gtcaaaaaag cctgatgata taaaagcgtt
     1621 aagaaatgaa caggagcata ttatttttgt ctgttctaaa tatgtaaagc caggcggagt
     1681 tttggtttat agcacatgta gtatagaacc aaaagaaaat gaagagcttg tcgaagactt
     1741 tttagcaaaa aaccaagaat ttgcttacga tgatttgagg aagtctttgc ctgaaatgct
     1801 tcatgatgaa ctaaaaagtc cttacggata tattactttg tacccgcata ttcacggcac
     1861 agacggcttt tttgtggccc gtctaaagag gctaaaaact tgaggggatg ccaagtgagc
     1921 aaaactaatt tgaagagttt aacactaaat caattggaag aatttatgaa gcaaatagct
     1981 gagccaacat atagggcaaa acaggttttc aagtggatat acaaagggga aacccagttt
     2041 gaaaagatga cggatttgcc gagaaattta atcaaaaaac tcaacgaacg tgcttatata
     2101 agcataattg gtaagtataa aaaacatgaa tccacattgg acggaactat taagtacgta
     2161 tttttactgg aagacgggaa catggtagaa agtgtaaaga tgatgtactc ctatggaatc
     2221 accgcatgca tttcgagcca ggtagggtgt cccatggggt gtactttttg tgcatcagcg
     2281 gaaggaggaa tggtgaggaa tttaacctgg tgggaaatgg cagatgaaat cctcgccata
     2341 gagagggatt ccggggataa agtaagtaga gttgttgtaa tgggaagtgg ggagcccctc
     2401 ttaaactatg atgagttgat acggttttta aatgtattgc acaatccaaa tgcttttaac
     2461 atgagtttca gaagaattac cgtatctact tgtggaatag tacctgagat ctaccgtcta
     2521 gccgatgagg gcattcccat tacccttgcc gtttcccttc acgctcccaa t
//
LOCUS       DAONUO010000289         2561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_27438_length_2561_cov_4.206305, whole genome shotgun sequence.
ACCESSION   DAONUO010000289 DAONUO010000000
VERSION     DAONUO010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27438_length_2561_cov_4.206305"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..515)
                     /locus_tag="PK768_09250"
     CDS             complement(72..515)
                     /locus_tag="PK768_09250"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQA61196.1"
                     /translation="MSTINEVDIYNYKIEIIKLSEEDGGGFLAAVPKLPGCMSDGDTP
                     DQALRNIQDAIKCWIETAKELGRKIPEPDEYKSEDDYSGKLTLRMPKMLHKLLAEQAE
                     KEGCSINQLILTYISIGIGNEFGRNQFSVGCDGDLYIHEKLTKKP"
     gene            complement(528..788)
                     /locus_tag="PK768_09255"
     CDS             complement(528..788)
                     /locus_tag="PK768_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQA61197.1"
                     /translation="MPHYKKLYEKIKNNPRDVRFEEIDKLLTKVGGFTRRNPGGGSSH
                     YTYSHPDSEDIITIPKDKPIKPIYIKKALRAFEIANEDFFII"
     gene            1105..1848
                     /locus_tag="PK768_09260"
     CDS             1105..1848
                     /locus_tag="PK768_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA61198.1"
                     /translation="MGAEYVTQYIKTNRTFTAIRKYGWLFTLLVAIGGLFEPKLGLLV
                     IFIMAGLMTTSFFSGRYWCGNICPHGSLFDTLLLSISRNSKIPEFLKSKYFILGFFVF
                     FMFNFSRKILSVFNQWGVYDFLDKLGFVFVSTYLMVLIVGGALAVFITPRTWCQFCPM
                     GTMQKLSYSLGKLVGVADKTETKVTISNPDKCIKCGKCSKVCPFQLTPYLEFDENNEF
                     SNIECIKCATCIENCPLKLLSLANREEAM"
     gene            complement(1926..>2561)
                     /locus_tag="PK768_09265"
     CDS             complement(1926..>2561)
                     /locus_tag="PK768_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HQA61199.1"
                     /translation="KGKGFMDKVLGAGKRVLAGESLFMTTFTNMGSGKKVVAFAAPYP
                     GKVIPVDLGECDGQLICQRDAFLCAAKGIAIEIAFQKKLGAGLFGGEGFIMQRLVGDG
                     LAFLHAGGTIIKKELQAGEVLKLDTGCLVAMTADIDYDVTFASNLQSALFGGEGLFLA
                     TLEGPGQVWLQSLPFSRVANRIYAAGKKGGGKVKSESNLLGGGLKNLLGGD"
BASE COUNT          702 a          543 c          505 g          811 t
ORIGIN      
        1 aacctggcaa acgaaacgtc cccctggcaa atctgacaaa cgaagcggcc cctggcagct
       61 ttttattcat cctatggttt ttttgttaat ttttcatgaa tatataggtc tccatcacag
      121 ccaacagaaa attgatttcg gccgaactca ttccctattc cgatagaaat ataagttaaa
      181 atcaattgat ttatactgca accttccttt tctgcttgtt ctgctaataa tttatgcaac
      241 attttcggca ttcttagtgt cagttttccg ctataatcat cttcagattt atattcatcc
      301 ggttcaggaa tttttcttcc taattctttt gcagtttcta tccaacattt gattgcatct
      361 tggatgtttc tcaatgcttg atcgggagtg tcgccatcgc tcatacaacc tggtagtttt
      421 ggcacagcag ctaaaaaacc gcctccatct tcctctgata gtttaattat ctctattttg
      481 taattataaa tatcaacttc attaatcgtg gacaacttat cccctcctta tataataaaa
      541 aaatcttcat ttgcaatttc aaaagccctt aatgcttttt taatataaat aggttttatt
      601 ggtttgtctt ttggaatggt tatgatatct tccgaatcgg gatgactata tgtataatga
      661 ctcgaaccac cacctggatt tcttcttgta aaacctccga ctttggtcaa cagcttatca
      721 atctcttcaa agcgcacatc tctaggattg tttttaatct tctcatacag cttcttataa
      781 tgaggcatga aaagtcctcc gattgtccct tataagtgat cttctatgca ttataacaac
      841 atatcagatt caaaatggta ttatatataa taccattaat ttttgttttt gtaaatattt
      901 gcttatggca aaatgaaaaa ttaaagagat gataagggga ctttgcaggg gacgccttga
      961 ttgttagatt gaaaacctgg caaacgaaac gtccccttgg catgttggca tttcccaaag
     1021 gcatgccgtc tttacgcgtg cgatattgac agcgaaatac aaaataagta taatcaaagc
     1081 tagattctac agaggaggta tactatgggc gcagaatatg ttacacaata tataaaaact
     1141 aatagaactt tcactgcaat tcgaaagtac ggatggcttt ttactttgtt ggtggccata
     1201 ggtggtttat ttgaaccgaa actcggcctt ctcgtgattt ttattatggc aggcctcatg
     1261 actacatctt tctttagcgg caggtattgg tgcggcaata tttgtcccca tggctcattg
     1321 tttgatacat tattattgtc tataagccga aatagtaaaa tacctgagtt tttaaaatca
     1381 aagtatttca ttctgggctt ttttgtattc tttatgttta atttttcaag gaaaatatta
     1441 agtgtgttta accagtgggg agtttatgat tttttagata aactcggatt tgtttttgta
     1501 tcaacatatt taatggtatt gatagtaggc ggcgcactgg ctgtgtttat tactccaagg
     1561 acctggtgcc agttttgccc catgggcacc atgcagaaat tatcttacag cctgggaaaa
     1621 ttggttggag tggccgataa gaccgaaaca aaagttacta tttccaatcc cgataaatgt
     1681 attaaatgcg gcaagtgttc aaaagtttgt cctttccaat taacccctta tttagagttt
     1741 gatgaaaaca atgaatttag caatatagag tgcataaaat gtgctacttg tattgaaaat
     1801 tgtccgctaa agcttttatc actggcaaac agagaggaag ccatgtagca acaagagacc
     1861 ttgtgcaggg tctacgagag ggggacgttt tcaatctggc aaacgaaacg tccccctgcc
     1921 aggttttagt ctccgccgag gaggtttttc aagccgcctc ccaggaggtt ggattcactc
     1981 ttgactttgc caccgccctt tttcccggct gcatagatgc ggttggccac ccggctgaag
     2041 ggaagggatt ggagccatac ctggccgggg ccttcaagag tggccaaaaa caagccctca
     2101 ccgccgaata gggcgctttg taaattgctg gcaaatgtca catcatagtc tatatctgca
     2161 gtcatcgcaa ctaaacaacc ggtatccagc ttgagaactt ctccggcctg aagttctttt
     2221 ttgataatcg tgcctccggc atggaggaag gccaggccat ctcctaccag gcgctgcatg
     2281 atgaagcctt ctccgccgaa taaacccgca cccagtttct tctggaaagc tatttcaatg
     2341 gcaatcccct tggcagcaca caagaatgca tctcgctggc agataagctg gccatcgcat
     2401 tcaccgaggt ctacgggaat gactttgccg gggtagggag cagcaaaggc tacaactttt
     2461 ttaccggaac ccatatttgt gaaggtggtc atgaagaggc tttcacctgc gagcacccgc
     2521 tttccggctc caagtacctt atccatgaag cctttgccct t
//
LOCUS       DAONUO010000290         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_27507_length_2556_cov_2.956817, whole genome shotgun sequence.
ACCESSION   DAONUO010000290 DAONUO010000000
VERSION     DAONUO010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27507_length_2556_cov_2.956817"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /gene="istA"
                     /locus_tag="PK768_09270"
     CDS             complement(<1..1108)
                     /gene="istA"
                     /locus_tag="PK768_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HQA61200.1"
                     /translation="MTQVKDIRKMYFEEGKNISEIARETGHDRKTVRGYLDKDNWNEE
                     PPKVKKEATFPKLDPYKPDIDTWLIEDKKAMRKQRHTAKRIYDRLVKKYKDGFNCSYR
                     TVAGYVAIKKKEIFGEKKGFLPLEHIPGEAQADFGDAQFYENGKLYSGKYFNLSFPNS
                     NKGYTQVYKGENQECLFEGLKTIFEHIGGVPTRIWFDNASTMVTKVMKGGGRNLTDDF
                     LRFMEHYRFEAAFCNVDAGHEKGNVENKVGYHRRNMLVPVPRFENLSEFNKDLLIQCE
                     EDAKREHYRKEGTIEEHYKDDAAALLELPKVAFETSKYITVKTNGYGRFLLNKGLHEY
                     SAAPKYANAHVLVKLTAFHVIVLDESHREIVRHER"
     gene            1694..>2556
                     /locus_tag="PK768_09275"
     CDS             1694..>2556
                     /locus_tag="PK768_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61201.1"
                     /translation="MIDPNIIRSFRNKVNSDSYFALNKYKNRNGKNHWNLICACMDWI
                     DLSVNYLLNNKIDNQNIDVMSMQVYTYISSIDIIWQSIQQLHRAIINVNTIPFDGEKQ
                     IFQNNSICKDDNDYFKHIRAVFGAHPVNLKDKNGKWFASWPTTGIYTEFDFAVLLYSA
                     EVENSDIVFGFKFTELNTFLQARYNYLEILGKELDKQYKEYIQEKTLIPINRSDDMLE
                     QLKILRQASKDRLDNDYYNHLIDDLIVIYEAESSLPENQETVDQYRSKLKELVDEITY
                     NLQHMRFGNLKT"
BASE COUNT          762 a          485 c          485 g          824 t
ORIGIN      
        1 gcctttcgtg gcgcactatc tcacggtggc tctcatcaag cactattaca tgaaaagcag
       61 tcagtttaac caatacatgc gcattcgcat acttaggggc tgccgagtat tcgtgtaagc
      121 ctttgttaag tagaaatctg ccatatccgt ttgtctttac cgttatgtat ttgcttgttt
      181 caaaggctac tttaggtaat tccaaaaggg ctgcagcgtc atccttgtaa tgctcttcaa
      241 tggtcccttc cttacggtaa tgctcccttt tagcatcttc ttcgcactgg attagaaggt
      301 ctttgttaaa ttcacttaaa ttctcaaacc ttggtaccgg taccagcatg tttcgcctgt
      361 gataccctac tttgttctct acattaccct tttcatgccc ggcatccacg ttgcaaaagg
      421 ctgcctcaaa ccggtaatgc tccataaacc gcaagaaatc atctgtcagg ttacgtcccc
      481 cacctttcat taccttggta accatggtgc ttgcattatc aaaccatatc cttgttggta
      541 ctccgcctat gtgctcaaat atcgttttta agccctcaaa caggcattcc tggttctctc
      601 ccttgtaaac ttgggtatac cccttattac tgttcggaaa cgataggtta aagtatttac
      661 cgctatacag cttgccgttt tcataaaatt gagcatcgcc aaagtccgcc tgtgcttcac
      721 caggtatatg ctctaagggc agaaaacctt tcttttcacc gaatatctct tttttcttta
      781 ttgctacata ccccgcaacg gttctgtagg aacagttgaa gccatccttg tacttcttta
      841 ccagccggtc gtatattcgt tttgccgtgt gcctttgctt acgcatggct ttcttatcct
      901 cgataagcca ggtatcaatg tccggcttgt atgggtcaag cttaggaaat gtagcttcct
      961 tcttcacttt tggcggctct tcattccagt tatctttgtc aaggtacccc cttactgttt
     1021 tacggtcgtg gccagtttct cgggctattt cactaatatt tttcccttcc tcaaagtaca
     1081 tttttctgat atccttaact tgggtcattg ttagcatcct ccagtcctcc ttgtacttga
     1141 tttgatctca aatacaagga tattatttag gtgtggtgcc ttcaatgacc tttttgcaat
     1201 tatgaggaat ttactgttgc aaaagtgggg aattcctttt gcaactttgt ccaattacat
     1261 tatgcaataa acagaattat ataaagcaat aagaacagtt tgttttgagc caagttgtat
     1321 aacaaaggag gaagtcttga atttccagac caagataagc gaattagaca agaaatcaac
     1381 taatttgcct ataagtttta ttggtagata ttgggcaaaa cgattaattg agaaagaaat
     1441 ggatcttagt tggataagaa agtatgagta acctgcacag caaataccaa caacggttcc
     1501 ctattcgaca cgaaaagtag gagcagtgta gggtgtggat ttagggcaag gctgaaatct
     1561 tagcgctaca cctttgccga atgcaagaac caccaagatg aagagctctg tggctggtta
     1621 gaggcgtagg gaagctgggt cgttatacta taccacactc tagtatattg tgactttctg
     1681 gggggggagg aaaatgatcg accctaatat aataaggagt ttcagaaata aagtgaatag
     1741 tgattcttat ttcgcattaa acaagtataa gaatagaaat ggtaaaaacc attggaattt
     1801 aatttgtgcc tgtatggact ggatagattt aagtgttaat tatttattga ataacaaaat
     1861 agataatcaa aatatagacg ttatgagtat gcaagtatat acatatatat cttcaattga
     1921 tatcatatgg caatcaattc agcaattaca tcgagcaata ataaatgtta atactattcc
     1981 ttttgatgga gaaaaacaaa ttttccaaaa taatagtatc tgtaaagacg ataatgatta
     2041 ttttaagcat ataagggctg tgtttggtgc gcatcctgtt aacttaaaag ataaaaacgg
     2101 aaagtggttt gctagttggc cgactactgg aatttatact gaatttgatt ttgcggtatt
     2161 attatatagt gctgaagttg aaaattctga tatagttttt ggtttcaagt ttacagagct
     2221 taacacattt ctccaggcaa gatacaatta tcttgaaata ctaggcaaag aattagataa
     2281 gcaatataaa gaatatattc aggaaaaaac attgattcct atcaatcgtt ccgatgatat
     2341 gctagaacaa ttgaaaatct taagacaagc gtcaaaagat aggctggaca atgattatta
     2401 taatcatctg attgatgatt taatagtcat ttatgaagca gagagttcac tacccgagaa
     2461 tcaagaaaca gttgatcaat atcgttctaa gctaaaagaa cttgttgatg aaataactta
     2521 taacttgcag catatgaggt ttggtaacct gaagac
//
LOCUS       DAONUO010000291         2555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_27516_length_2555_cov_3.646000, whole genome shotgun sequence.
ACCESSION   DAONUO010000291 DAONUO010000000
VERSION     DAONUO010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27516_length_2555_cov_3.646000"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PK768_09280"
     CDS             <1..425
                     /locus_tag="PK768_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQA61202.1"
                     /translation="AGDFEDPAKGKECALALYSKGADIVFHAAGKTGEGVFEAAKEVK
                     AYAIGVDSDQRYINPDVIVASMIKGVGLSVFETIKKIQEGSFEPGKVIYYGINEGGVD
                     VGYGTPDMKQIVPDEIKAKVEEIKAKVKSGEIEVPTAK"
     gene            501..2033
                     /locus_tag="PK768_09285"
     CDS             501..2033
                     /locus_tag="PK768_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA61203.1"
                     /translation="MGESIIKLVGITKVFPGVVANDNVNLEIREGEIHAIVGENGAGK
                     STLMKIIYGLYQPTDGQIYIRGRLTNVTNPQVAIKNGIGMVHQHFMLVPSFTAAENLV
                     LGSEPQKNGLFMDFDKAVKITKELSEKYGLYVDPYQKVEDLSVGIQQRIEILKVLYRG
                     ADILILDEPTAVLTPQESQELFAIMRRLVEDMGKTIIIITHKLQEVLEVSHRVSVMRR
                     GKLVGTMDTRDATEQILAEMMVGKEVVFEKFHANQNIGEKLLEVRDLVVRDNRGFIAV
                     DGVSFSIAAGEILGVAGVEGNGQTELVEALTGLRKLEEGSIFVKGRDMAKGSPSDFRR
                     MGIAHIPEDRLKTGLAPEASVNENLMMGYQHKKPYEGLGMKLNKKAISQKTAQLIRQF
                     DIRTPSGQTRVKSLSGGNMQKLVVAREFSFDSPILIIAQPTRGLDVGAIEFIHRLIID
                     MRDAGRAILLVSADLDEIFRLSDRIVTIYEGKLTGEFRTADVTKQEIGLYMTGKNLST
                     AG"
     gene            2038..>2555
                     /locus_tag="PK768_09290"
     CDS             2038..>2555
                     /locus_tag="PK768_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61204.1"
                     /translation="MKKLKLSQDLEYIFSLALSVILALIIGAIIILANGKSPFTAYAA
                     LINGALGSPYKIATTFAKTVPLILTGLATAVAFSSGIFNIGGEGQLYLGAFAAAYVGF
                     TFTNLPGIAGIPLALIFAALAGGFYALVPALLKVKYGIDEVITTIMFNSIATLFTSYL
                     ANYPFASSVGKMG"
BASE COUNT          706 a          609 c          687 g          553 t
ORIGIN      
        1 atgcaggcga ctttgaagac ccggcaaagg gcaaggaatg cgcactggcc ctttactcca
       61 agggcgctga catagtcttc catgctgccg gcaagaccgg ggagggtgtt tttgaagccg
      121 caaaagaggt caaggcctat gccataggcg tagattcgga ccagaggtat ataaatcccg
      181 atgtcatagt ggccagcatg attaaaggcg taggactctc ggtcttcgaa accataaaga
      241 aaatccagga aggcagcttc gagccgggca aggtcatcta ctacggcatc aatgagggcg
      301 gcgtggatgt cggctacggc actcccgaca tgaagcagat tgtccccgat gaaatcaagg
      361 cgaaagttga ggaaataaaa gcaaaagtca agagcggcga aattgaagtg cctacagcaa
      421 aataagctaa aacccgcata taaaggagaa gtaaccttac ttctcctttt ttaaagaaaa
      481 gctttgaggc aggtgctagt atgggagaaa gtataattaa gctggtggga atcaccaagg
      541 tctttccggg agttgtggca aatgacaatg ttaacctgga aataagggaa ggggaaatac
      601 acgccatagt cggggaaaac ggcgccggca aatccaccct catgaaaatt atttacggcc
      661 tgtaccagcc gactgatggc caaatttaca taagaggccg acttacgaat gtcacaaatc
      721 cccaagtcgc cataaaaaac ggcataggca tggtccacca acactttatg ctggtcccct
      781 cttttactgc ggcggaaaac ctggtcctgg gaagcgagcc tcaaaaaaat ggccttttca
      841 tggactttga taaagcggtg aaaataacca aggaactctc cgaaaagtac ggcctttatg
      901 tggaccccta ccagaaggtg gaagacctgt cggtgggcat acagcagcgc atcgaaatat
      961 tgaaggtcct ttacagggga gccgatattt tgattttgga tgagcctact gcggtcttga
     1021 ccccccagga aagccaggaa ctctttgcca tcatgcggcg gctggtagaa gacatgggca
     1081 agaccatcat cataatcacc cacaagctcc aggaggtgct ggaggtttcc cacagggtca
     1141 gcgtcatgcg caggggaaaa cttgtgggca ccatggacac cagggatgcc accgaacaga
     1201 tactagcgga aatgatggtg ggcaaggaag tggtctttga aaaatttcat gccaaccaaa
     1261 atatcggcga aaagctgttg gaagtaaggg atttggtggt aagggacaac cggggcttta
     1321 ttgccgtaga cggagttagc ttttccattg ccgccggcga aatcctaggc gttgcaggag
     1381 tggaggggaa tggtcagacg gaactggtgg aagccctgac gggcctcagg aagcttgagg
     1441 aagggagcat attcgttaag ggaagggaca tggcaaaggg ctctccttca gacttccgca
     1501 ggatgggcat cgctcacatt cccgaagata ggcttaaaac cggtctcgca ccggaagctt
     1561 cggtaaacga gaatttgatg atgggatatc agcacaaaaa gccctatgag ggccttggga
     1621 tgaaattaaa caaaaaggcc atatcccaaa agaccgctca gctcatccgg cagtttgaca
     1681 tccgaacacc ttccggccag accagggtga agagcctttc cgggggcaac atgcaaaagc
     1741 ttgtcgttgc tagggagttt tcctttgatt ccccgatcct cataattgca cagcccaccc
     1801 gcggcctgga tgtgggagcc attgagttta tccaccgcct gataatcgac atgagggatg
     1861 cgggcagggc catactactg gtatcggcgg acctggatga gatttttaga ttatccgaca
     1921 ggatcgtgac catatacgaa gggaaactga cgggagagtt tagaactgct gatgtgacga
     1981 agcaagaaat aggcctttac atgaccggca aaaatttatc cacggcgggg tgatggtatg
     2041 aagaaattaa agctatccca ggacctggaa tacatctttt cgctggcctt atccgttatc
     2101 ctcgcactaa taatcggtgc gattataatc cttgccaacg gcaaaagtcc ctttaccgcg
     2161 tacgccgccc ttataaacgg cgccttaggc agtccctata aaatcgccac tacctttgcc
     2221 aaaaccgtgc ccctgatctt gacgggcctg gccacggctg tggcctttag cagcgggatt
     2281 ttcaacatcg gcggcgaagg gcagctgtac ctcggggcct ttgccgcagc gtacgtggga
     2341 tttaccttta caaatctgcc cggcatagca ggcataccac tggcccttat ctttgcagcc
     2401 ctggccggcg gtttttatgc cctggtaccg gcactgctaa aagtgaaata tggaattgac
     2461 gaagtcatca cgactatcat gtttaacagt atcgccacac tgtttaccag ctaccttgcc
     2521 aattacccct ttgcatcttc tgtaggcaag atggg
//
LOCUS       DAONUO010000292         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_27618_length_2547_cov_3.648876, whole genome shotgun sequence.
ACCESSION   DAONUO010000292 DAONUO010000000
VERSION     DAONUO010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27618_length_2547_cov_3.648876"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /gene="tyrS"
                     /locus_tag="PK768_09295"
     CDS             <1..705
                     /gene="tyrS"
                     /locus_tag="PK768_09295"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQA61205.1"
                     /translation="FYYLHKNKNCRLQMGGNDQWSNIISGVDLIRRVEGASAYGMTFT
                     LLTTSEGVKMGKTQAGALWLDPEKVSPYDFYQYWRNVDDRDVEKCLKLLTFLPMDEIK
                     RLSSLEGAEINKAKEVLAFEVTKLIHGEEEAIKAQNAARALFRDGADLTSVPQTQISR
                     EQIAEGMEILELLMLTGLTASKSEGRRLIKQGGIYVANSRVDDIDMKITAKDFDEDSS
                     LLLRKGKKVYHMVKLS"
     gene            complement(807..2276)
                     /locus_tag="PK768_09300"
     CDS             complement(807..2276)
                     /locus_tag="PK768_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA61206.1"
                     /translation="MQTYDVIIIGAGIVGTSIARNLSRFKLDILLLEKANDVGMGSTK
                     ANSAIVHGGYAEKYTALKGRLCYKGRVQFKRLNEELNFGFAQTGSLVVTTEDDTKPLE
                     ELMENGIKNGLHDLSIIGAEKIRQLEPELSRDIKYALYCEGAGVCSPYEMAIALAENA
                     VKNGVELKLEEEVTSIEKVDGVFRVTVNEKKEYLGRYVINAAGIYADKVSKMVGVDDF
                     EILPRSGQFMIFTRGSGDPINTVVFQLPTKLGKGVLLTSTYYGNLLLGPDAKDDGERE
                     DTSTHVERLAEIYRQTKALYDKLDPRLFIRSFTGIRARSSTDDFIIGETRVPGFINVA
                     GIQSPGITSSPAIADMVVDILSKAGLRLEENPNFDPYRKPIITKKPLRPYKEIKDLLN
                     IPSAPEKIICRCEQVTEAEIVDALHRGIEVKTVDGVKRRTRAGMGWCQGNFCRSRVVE
                     IMEREYKKRIDPSDDVERSGVKRVTKSELLEYLKSQQLL"
BASE COUNT          691 a          632 c          537 g          687 t
ORIGIN      
        1 ttttactacc tccataaaaa caaaaactgc aggctgcaga tgggcggcaa tgaccagtgg
       61 tccaacatca tcagcggtgt cgatttaata aggcgggttg aaggtgcctc ggcctacggc
      121 atgaccttta ccctccttac caccagcgaa ggtgtcaaaa tgggcaagac ccaggccggt
      181 gcactatggc tggaccctga aaaggtatcg ccctacgact tttatcagta ctggagaaac
      241 gtggacgaca gggatgtgga gaagtgcctg aaactcctca ccttcctgcc catggatgag
      301 ataaagcgcc tgagcagcct ggaaggcgcc gaaataaaca aggccaaaga agtgctggct
      361 tttgaggtca ctaaactgat acacggcgag gaagaggcta taaaggccca aaatgcagca
      421 agggccctct tccgagacgg tgcagacctg acatctgtgc ctcaaactca aatcagtcgg
      481 gagcaaatag ctgagggcat ggaaatactt gaacttttga tgctgaccgg cctcacagct
      541 tcaaagagcg agggcagaag gctcataaag cagggcggga tttacgtcgc aaattcccga
      601 gtagatgata tagacatgaa gattaccgca aaggattttg atgaagatag cagcttgcta
      661 ctaagaaaag gcaaaaaggt ctatcacatg gtaaaactgt cttaaatata aatgggtgcc
      721 ttgatataga accgactatt atcaagacac ccattgtttt atctcaacac cggcacttac
      781 gtagccgtca ccgcaatgaa acaaattcat agtagttgct ggcttttcaa gtattccagc
      841 agctcggatt ttgttaccct ttttacgccg ctgcgctcta cgtcatcaga tgggtctatt
      901 ctctttttgt attctctttc cattatttca acaacccttg acctgcaaaa attcccctgg
      961 caccagccca tgccggcccg ggttcgcctc tttactccat ccacggtctt aacctctatc
     1021 cccctatgaa gggcatctac aatttcggct tcggtgacct gctcgcacct gcatatgatt
     1081 ttttcagggg ctgatgggat gttaagcaaa tccttaattt ccttataagg tcgcagaggc
     1141 ttcttggtga tgataggctt tctgtaggga tcaaagtttg gattttcttc cagcctaaga
     1201 cctgccttgc ttaaaatgtc aaccaccata tccgcaatgg ccggagaaga tgtaatgccc
     1261 ggagactgga tgccggctac atttataaag ccggggacgc gggtttctcc tatgataaaa
     1321 tcgtccgtag aactcctggc cctgatgccg gtaaaactcc tgatgaaaag cctcgggtca
     1381 agcttatcgt aaagggcctt ggtttgccta tagatttcag ctaagcgctc cacgtgggtg
     1441 gaggtgtctt ccctttcccc atcgtccttt gcatcaggcc caagtagcag attcccgtag
     1501 tatgtagagg tcaacagcac gccttttccg agcttcgtcg gcaactgaaa caccaccgtg
     1561 ttgatgggat cacctgaccc tctagtaaaa atcataaatt ggccgcttct gggaagaatc
     1621 tcaaagtcat ctacccctac catcttggat actttatctg cataaattcc tgccgcatta
     1681 atgacatacc tgccgagata ctccttcttt tcgtttacgg tcaccctaaa aacaccatct
     1741 actttctcaa tagaggttac ttcctcttcc agcttcagct cgacgccgtt tttcactgca
     1801 ttttctgcca aagcgatggc catttcatag ggagagcaaa ccccagcacc ctcacagtac
     1861 agtgcgtatt ttatgtcacg ggacagctcc ggttccaatt gcctaatctt ttcagctcct
     1921 attattgata agtcatgtag gccgtttttt atgccatttt ccatgagctc ttccaggggc
     1981 ttggtgtcat cttccgtagt aacaaccaga gagccggttt gggcaaatcc gaaattcagc
     2041 tcttcattca gccgcttaaa ctggaccctg cccttatagc acaatctccc tttaagagcg
     2101 gtatacttct cagcataacc cccatggaca atggcactat tagccttggt tgaacccatg
     2161 cccacatcat tggccttttc cagcagcaat atatccaact taaagcggga gagattcctt
     2221 gcgatggacg tgcccactat tccagcacct attataatga catcgtaggt ttgcatatca
     2281 aaacccccta tattttttgg caatatatta agaattgatt ttattatatt catatttctt
     2341 acaatatgca aggacgagtc cataaactcc gacatgatat ttcataatca gacacggcca
     2401 gcaccgaata tgcaaatcat aaaaaggatt tggcgtttta tccataatta ttgaagtata
     2461 atattttgta atagcaacag aattcttgaa ccataaatag attcaaaaaa gcgcaggaaa
     2521 cgaaatttta tcatagggtg tctattt
//
LOCUS       DAONUO010000293         2490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_28396_length_2490_cov_3.303901, whole genome shotgun sequence.
ACCESSION   DAONUO010000293 DAONUO010000000
VERSION     DAONUO010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28396_length_2490_cov_3.303901"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PK768_09305"
     CDS             <1..392
                     /locus_tag="PK768_09305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQA61207.1"
                     /translation="GSIKMVKLSTEFTGRQTLYLSFDEIDFHEEFDGVWACASLLHVP
                     RNRIDSIFKKVYIALKKGGVFYSSYKYGNREEIKNGRFFNNYDEPLFKELLKKHPYFV
                     LMEMKVTNDVRIGRENEKWLNVVLRKT"
     gene            643..1320
                     /locus_tag="PK768_09310"
     CDS             643..1320
                     /locus_tag="PK768_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HQA61208.1"
                     /translation="MLFLLLMMMIFMFFPYLFLPILLFLAIGFLFLLPYVFLFNSFYN
                     VVTIPWQILKIAVDKRVRKNHSLEHATINVLEERYGRSLQIGGLAYSNGFSLSGPELP
                     PPYEVMSAVREGHFRMINGEKDLAAHPRCGTSIAAANFLFSLAFIIVLFFSRHLTLLN
                     VIAAFLLANILAKPFGRLLQKLFTTYSIVDDVVIQDIYIQPSNIGFSFIFMPNPNRSY
                     FIKTYQS"
     gene            1412..1696
                     /gene="gatC"
                     /locus_tag="PK768_09315"
     CDS             1412..1696
                     /gene="gatC"
                     /locus_tag="PK768_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQA61209.1"
                     /translation="MDKDTLERMADIAHLHLETREIEQILHDLGPLLKQFDKLNQADT
                     EGIATTVYPINDKNVLREDEVWESLPIHEVLKNAPDRDNRFFRVPRIIEE"
     gene            1716..>2490
                     /locus_tag="PK768_09320"
     CDS             1716..>2490
                     /locus_tag="PK768_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQA61210.1"
                     /translation="MLELDKLSIHIAHEMLIKKEVSSEELTKAFFERIEDVDEKTKAY
                     VTLNGDKALEESREIDKSGDIKEYLAGIPLAVKDNICTKDLRTTCSSKMLENFVPPYD
                     ATVIAKLKDKNAIVLGKTNMDEFALGSSTENSYFFTSKNPWNLERVPGSSASAAAVAA
                     SEALYALDSDTGGALRQSASFCGVVGLKPTYGRVSRFGLAATASSMDTVGPIAKNVKD
                     CAIVLNAISGIDSFDPTSSDAVVPDFLKSLESKPEGAKIG"
BASE COUNT          764 a          467 c          531 g          728 t
ORIGIN      
        1 atggttcgat taaaatggtc aagctttcaa cagagtttac ggggaggcaa accctttact
       61 tgtcatttga tgagatagac tttcatgaag aatttgatgg ggtatgggca tgtgcttcgc
      121 tattgcatgt gccgagaaac aggatagatt ctatatttaa aaaggtctat atagcactga
      181 aaaagggcgg tgttttttat tcttcatata aatacgggaa ccgggaagaa ataaaaaatg
      241 gacgcttttt caacaactac gatgaacctt tattcaaaga actcttaaaa aaacatcctt
      301 attttgtcct aatggaaatg aaagtgacca atgacgtgag aataggaagg gaaaatgaaa
      361 aatggttaaa tgttgtttta cggaagacat agtgctttga ggaaatgtat tcacttatat
      421 aattaggctt acttttgttc tcggccataa aatcaatatg atatatgaac gattcgatag
      481 aattcttcac ccctgtgtga ggaaaaattt ttgcaaaatg tggagtctcc ccttgcaaaa
      541 ataagcaaaa agttctccat cctaaagtgt gataaaagct acagcgaaat tgacagcatt
      601 tgtgttataa ttaatttaag taaatggaaa ggggattgtt tgatgctatt tctactttta
      661 atgatgatga tatttatgtt ttttccatat ttgtttttgc ctattttatt atttctagcc
      721 atcggatttt tatttttgct cccttacgtt tttttgttta attcttttta caacgtagta
      781 accatcccct ggcaaatttt aaagattgct gtagacaaga gggtcaggaa aaatcacagc
      841 ctggaacatg ctaccataaa tgttctggaa gagaggtacg gcaggtctct gcaaataggg
      901 ggccttgcct attcgaacgg tttttcgctt tcaggacctg aacttccgcc accctatgaa
      961 gtaatgagtg cggtccggga aggccatttc cgcatgataa acggtgaaaa agacttggct
     1021 gcccatcccc gctgcggaac atctatagcg gctgctaact ttttattttc ccttgcattt
     1081 attatagtgc tgttcttctc ccgccatctt acattattaa atgtaattgc cgcttttctg
     1141 ctcgcaaata tactggcaaa gcctttcggt agattgttgc aaaaattatt taccacatat
     1201 agtattgtcg atgatgtggt aatacaggat atctatatcc agccaagcaa tattggtttt
     1261 tcctttatat ttatgccaaa cccaaacagg tcctatttta tcaaaaccta tcaatcatga
     1321 ttagagtgcc ctcgcaaata cgttgtataa tataccatta aatgataaaa ttataaagga
     1381 cttgcaattt aataagagag gtgatttgat agtggataag gatacactgg agcgcatggc
     1441 ggatattgct cacctgcatc ttgaaacccg agaaatagag caaattttac atgatctggg
     1501 gccactccta aagcagtttg ataagctaaa tcaggcagat accgagggaa ttgcaactac
     1561 agtttaccca ataaacgata agaacgtgct tagggaagat gaggtttggg aaagccttcc
     1621 tatccatgaa gttttgaaga atgcacctga tagggacaat agatttttca gagtgccgag
     1681 gataattgag gaatagattt tttacgggag gtaatatgtt ggaactggat aagctgtcga
     1741 ttcatatagc tcatgagatg ctcatcaaaa aggaagtaag ttctgaggaa cttaccaaag
     1801 ccttttttga aagaattgaa gatgtagacg aaaagacaaa ggcttacgtc actttaaacg
     1861 gcgacaaagc tttggaagaa agccgcgaaa ttgataaaag cggtgatatc aaagaatatc
     1921 tagcgggaat tccgctagcc gtaaaagata atatttgcac taaggatttg aggaccacct
     1981 gtagctcaaa aatgctggaa aactttgtac caccttacga tgccacagtc atagccaagc
     2041 tcaaagataa aaacgcgatt gtcttaggca aaaccaatat ggatgagttt gccttgggtt
     2101 cctctacgga aaactcctat ttctttacca gcaaaaaccc atggaatctc gagcgggtgc
     2161 ccggaagctc ggcttctgct gctgccgttg ctgcctccga ggcgctgtat gcactggatt
     2221 cggacacggg cggagcctta agacagtcag ctagcttctg cggagtcgtg ggcctaaagc
     2281 ctacctacgg cagagtatcg cgctttggcc ttgcagcaac tgcctcatcc atggatacgg
     2341 taggccccat cgctaagaat gttaaagact gcgccatcgt tttaaatgcc atttcaggca
     2401 ttgacagctt tgatcccaca tcatctgatg cagtagttcc cgatttccta aaatcactag
     2461 aatcaaagcc tgaaggggca aagattggca
//
LOCUS       DAONUO010000294         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_28410_length_2489_cov_3.665571, whole genome shotgun sequence.
ACCESSION   DAONUO010000294 DAONUO010000000
VERSION     DAONUO010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28410_length_2489_cov_3.665571"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK768_09325"
     CDS             complement(<1..712)
                     /locus_tag="PK768_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HQA61211.1"
                     /translation="MNFYDFHVHLEEGPFTIDWLRRFIEVGEKRGVVEIGFTEHGHRF
                     KESVGLLASDGFRGSWTKGEATESIEDYIEIVQKAKDMGLPVKLGIELDYVPEFEEQI
                     KEFAKSHPFDYVLGGIHWLGDFGFDHPDLIHEWDEKDVDEVYVEYFNTLLAAVKSGIY
                     EAIAHPDVIKVFGYKARRDMSEYYDELTKAVKEMDICVEVSTAGLRKPVGELYPSPEL
                     MKYFKKYEVPIMISSDAHV"
     gene            complement(844..1152)
                     /locus_tag="PK768_09330"
     CDS             complement(844..1152)
                     /locus_tag="PK768_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HQA61212.1"
                     /translation="MAFQDKTIVCKECGTEFVFTAGEQEFYAEKGFENEPSRCKACRD
                     ARKRRMNEGGFRRQNRQMYDAVCADCGAATQVPFKPRDDRPVYCSDCYQNHRMAQSGY
                     "
     gene            complement(1365..1805)
                     /gene="flgN"
                     /locus_tag="PK768_09335"
     CDS             complement(1365..1805)
                     /gene="flgN"
                     /locus_tag="PK768_09335"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HQA61213.1"
                     /translation="MDREITSLLEQKLDKINELLKVTQSISDIIKKEDIEELLKLFKD
                     RQQLMDDIDHIDGKLLTFFKDDKNALVKHINSDGQLKAINDSVLSLVTRIKALDDANL
                     EGVKNLFAKLKEDIAAFRQTETALKGYGLLGTSNRDGAFIDTKK"
     gene            complement(1830..2210)
                     /gene="fliS"
                     /locus_tag="PK768_09340"
     CDS             complement(1830..2210)
                     /gene="fliS"
                     /locus_tag="PK768_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HQA61214.1"
                     /translation="MINANAYQQYQYNSIMSASPERLMLMLFEGAIKFVKLARIAIEE
                     KDIERANYNLTRAQDIIAELNQSLDMSFDISESLAGIYDFLYRQLVDANIKKDLDALD
                     VVESMLVELKETWEEAYLSLRKSV"
     gene            complement(2238..>2489)
                     /gene="fliD"
                     /locus_tag="PK768_09345"
     CDS             complement(2238..>2489)
                     /gene="fliD"
                     /locus_tag="PK768_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HQA61215.1"
                     /translation="ILKGGIEDITEKAGGGEFQIYDDSILGKQIRDMEDRIASMQEML
                     IRTEERYWQKFIAMEQAIQYANQQSLWLMTQMNMYTGGY"
BASE COUNT          652 a          641 c          422 g          774 t
ORIGIN      
        1 gcacatgggc atcagaactt atcataatgg gtacttcata ctttttaaaa tacttcatga
       61 gttcaggtga tgggtaaagc tcccccacgg gctttcgaag gccggccgtg ctgacttcca
      121 cacatatgtc catttccttc acggcttttg tcagctcatc gtaatactcg ctcatatccc
      181 gcctcgcctt atagccgaag acctttatca catccggatg ggcaatggcc tcataaatcc
      241 ctgattttac ggcagcaaga agtgtattaa aatattcaac gtagacttca tccacatctt
      301 tctcgtccca ttcatgaatc aagtcagggt ggtcaaagcc aaaatcccca agccaatgta
      361 tgccgcccag cacatagtca aagggatggg attttgcaaa ctcctttatc tgctcttcaa
      421 actccgggac ataatctagt tcgatgccta gttttactgg cagacccatg tctttagcct
      481 tctgaactat ttctatgtag tcttcgatac tctccgttgc ctctcccttc gtccagctgc
      541 ccctaaagcc gtcactggcc aaaagcccca ccgactcttt aaatctatgc ccatgctcgg
      601 taaagcctat ttcaacaacc cctctttttt cacctacctc aataaaccga cgcagccagt
      661 ctatagtaaa tggtccttcc tcaagatgca catgaaaatc gtaaaagttc atataatcac
      721 cctatagagt atttctcaca tcaaatattt tagcacaagc atgcaaaaaa atatataaaa
      781 ccgaaacaaa aaatcgtgta aaaacaaaaa agtccggaat gataccggac ttattttccc
      841 ttcttaatat ccgctttggg ccatgcgatg gttttggtag caatcactac agtatacggg
      901 tctatcgtct ctgggcttaa agggaacctg tgtggctgca ccgcagtctg cgcaaactgc
      961 atcgtacatc tgcctgtttt gccttctaaa gccgccctcg ttcattctcc tcttgcgcgc
     1021 atcacggcag gccttgcatc tggatggttc gttttcaaag cccttttctg cataaaactc
     1081 ctgctcacct gcggtgaaaa cgaactcagt accacactct ttacacacta ttgttttgtc
     1141 ctgaaatgcc attaaaaaaa tccctcactt taaaaaagtt ttcttggcat ttccttagct
     1201 gacctggtct taccccaaaa aagccgcata cttggagtgg attgccaccc tgggagtcgc
     1261 ttcgcctccg gccctggcat cgactttcaa ccacagacct aagtaatgcc tatgtttgat
     1321 tataacctta aatgtaataa aatgcaagaa aaattaataa agagctattt tttcgtatct
     1381 ataaaggcac catcccgatt gcttgtgccc aaaaggccat aacctttgag ggcagtttct
     1441 gtttgccgaa aagcggcaat gtcttccttt agcttggcaa ataagttctt gaccccctca
     1501 agatttgcat catccagtgc ctttattctc gttactaaag acaaaacgct atcgttaata
     1561 gctttcaatt ggccgtcact gtttatatgt tttacaagcg catttttgtc atctttaaaa
     1621 aaagtcagca gcttgccatc tatatggtca atatcatcca tcagctgctg cctatcttta
     1681 aataatttca acaactcctc gatgtcttct ttttttataa tatcggatat gctttgagtg
     1741 acttttaaaa gctcatttat cttatcaagt ttttgttcca acaagctcgt tatttccctg
     1801 tccatttaac tctcccaaac aatttatatt tatacgcttt tcctcaagct aagataagcc
     1861 tcttcccagg tttctttaag ctccaccaac atggattcga caacatccaa agcatctaga
     1921 tctttcttga tatttgcatc gactagctgc ctgtacaaaa agtcgtatat tcctgccaaa
     1981 ctctccgata tgtcaaagct catgtccaaa ctttgattga gctctgctat gatgtcctgg
     2041 gctctagtaa gattgtagtt cgccctttct atatcctttt cttcaatggc tatccgggca
     2101 agtttcacaa actttatagc accctcaaat agcatcagca tcaagcgctc gggagaagca
     2161 gacattattg agttgtactg gtactgttga taagcattgg catttatcat aaatgagccc
     2221 ccttttcctc gcaattatta atacccgcct gtgtacatgt tcatctgcgt catgagccac
     2281 aggctctgct gattggcata ctgtatggcc tgttccatgg cgatgaattt ctgccagtaa
     2341 cgttcttccg tgcgtatcag catttcctgc atgctggcaa tgcggtcttc catatcccgg
     2401 atctgctttc caagaatact gtcatcgtat atttgaaatt cgccgccacc ggctttttcc
     2461 gttatatctt caattccgcc ttttaatat
//
LOCUS       DAONUO010000295         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_28468_length_2485_cov_3.656379, whole genome shotgun sequence.
ACCESSION   DAONUO010000295 DAONUO010000000
VERSION     DAONUO010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28468_length_2485_cov_3.656379"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PK768_09350"
     CDS             complement(<1..136)
                     /locus_tag="PK768_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HQA61216.1"
                     /translation="MSFDGIVLYGIIHELQQSLLDGRISRIYQPSKNELVLFIIHNRR
                     E"
     gene            322..1194
                     /locus_tag="PK768_09355"
     CDS             322..1194
                     /locus_tag="PK768_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQA61217.1"
                     /translation="MIKSMTGYGRGENQGKFHFTVEIRSVNHRFLELFVRLPKPWLSL
                     EDKIKNYVRGKIKRGRLDVYVNMHDESLPVDIKIDKSLVNNYYKKLMELKNEIGFEGQ
                     ISLSLLSLLPNVFILEEDSMVEDDLWESLAPALDEALDNLIKMRTKEGGNLWKDLNAR
                     LELIKDKVELIKERADMIPKEYKERLMQNVQRLSSDLSFDEDRIETEAVIFAEKSNIT
                     EEVVRLGSHLAQMQELLYSDDAVGKKMDFIAQEMFREANTISVKASDYCVSKEIIDIK
                     SELEKIREQIQNIE"
     gene            1214..1498
                     /locus_tag="PK768_09360"
     CDS             1214..1498
                     /locus_tag="PK768_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HQA61218.1"
                     /translation="MDIKLVNIGFGNIVSANRIIAIVSPESAPIKRIIQEARDRGMLI
                     DATYGRRTRAVIITDSDHVVLSAVQPETVANRLSNDKHAMEDEEENGELE"
     gene            1485..2129
                     /gene="gmk"
                     /locus_tag="PK768_09365"
     CDS             1485..2129
                     /gene="gmk"
                     /locus_tag="PK768_09365"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQA61219.1"
                     /translation="MNWSNTDKGMLIVISGPSGVGKGTLCKILLNRVENLFLSISATT
                     RPPRPGEIDGRDYIFLSRELFEEKIKKGEFLEWAKVYNNYYGTPKDIVFQQLRDGKDV
                     VLEIDTQGAAQVKNNYPEGIFIFISPPSIKELKKRIIKRGSDTESSIELRMKSAIDEI
                     KAASEYDYIVLNDDLNQAALNLQSIVLAERCKVSRNTKIVLKLREEFGNDASVN"
     gene            2110..2316
                     /gene="rpoZ"
                     /locus_tag="PK768_09370"
     CDS             2110..2316
                     /gene="rpoZ"
                     /locus_tag="PK768_09370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQA61220.1"
                     /translation="MMHPSIDSLTEKYDSKYTIAVAAAKRARQLVEGAKELVDVKTKK
                     PVSLALFELDAQKIVVYRPTTGKK"
     gene            2329..>2485
                     /locus_tag="PK768_09375"
     CDS             2329..>2485
                     /locus_tag="PK768_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HQA61221.1"
                     /translation="MLQGKFVVLGVTGSIAAYKSVELVRLLKKAGANVQVIMTKSGCK
                     FIKPLTFQ"
BASE COUNT          874 a          392 c          546 g          673 t
ORIGIN      
        1 gttcccttct attatgaatg ataaaaagga caagttcatt tttgctaggc tgataaatcc
       61 tgcttattct gccatcaagc agtgattgct gaagttcatg gattataccg tatagtacta
      121 tcccatcaaa cgacataaaa caacctccct attgaatcat agtatagcac ttgcgacaag
      181 cttttggaag gtcaatattt tgttgtttaa aacataataa aatgatataa taattgatac
      241 gattattaag aaaatacaag aaaaccaggg attagccctg gtttaatgga tttatcattg
      301 ttaacgtaaa aggggattgt aatgataaaa agcatgaccg gttatggccg aggtgaaaat
      361 caaggcaaat ttcattttac tgtggagatt aggtcggtaa atcatagatt tttagagcta
      421 tttgtaagat taccgaaacc ctggctttct ttggaggata agataaaaaa ctatgtgagg
      481 ggaaaaataa aacgaggcag actggatgta tatgttaata tgcatgatga gagtctcccc
      541 gttgatataa aaattgacaa aagccttgtt aacaattact ataaaaaatt gatggaacta
      601 aaaaatgaaa taggctttga agggcaaatt tcactgtcac tgctttctct tttgccaaat
      661 gtttttatct tggaagaaga ctccatggta gaagatgact tatgggaatc tttagcgcct
      721 gccttggatg aggctttgga taatctcatt aaaatgcgta caaaggaggg gggaaacctc
      781 tggaaagact taaacgcaag gttggaactc ataaaagata aagtagagct aataaaagaa
      841 cgagcagata tgattcctaa agagtataaa gagaggctca tgcagaacgt tcaaagattg
      901 tcttcagatc tttcctttga tgaagataga atagagacag aagcggtaat atttgcggaa
      961 aaatccaata ttaccgaaga agttgtgaga cttgggagcc atctagctca aatgcaggaa
     1021 ttattatact cggacgatgc tgtaggcaaa aaaatggatt tcattgctca agaaatgttt
     1081 cgcgaggcaa acacgatttc agtaaaagca tcggattatt gtgtatcaaa agaaataatt
     1141 gacattaaga gcgaactgga aaaaatacga gaacaaattc aaaatataga atgaactttc
     1201 aggaggtata agtgtggata ttaaacttgt taatattggt tttggcaata ttgtgtcggc
     1261 aaatcgaatc atcgctatag ttagccctga atctgctcct attaagcgaa ttatccaaga
     1321 agcaagagat cgagggatgt taattgatgc aacatatggc aggaggaccc gtgcggttat
     1381 aataactgac agtgaccatg tagtgctatc agcggttcag cctgaaacgg ttgcaaaccg
     1441 attgagcaat gataaacacg caatggaaga tgaagaggaa aatggtgaat tggagtaaca
     1501 cagataaagg aatgttaatt gtcatatcag gtccttccgg cgtagggaag gggacactat
     1561 gtaaaatact tttaaataga gtagaaaatc tctttctttc catttccgcc acaacaagac
     1621 cgcccaggcc cggagagatc gatggtcgtg attacatttt tttgagccgt gagctttttg
     1681 aagaaaaaat aaaaaaaggt gaatttcttg aatgggccaa ggtttataat aactactacg
     1741 gcaccccaaa ggatatcgta tttcaacaac ttcgtgatgg taaagacgtt gtgcttgaaa
     1801 tagacaccca gggtgcagcc caggtaaaga ataattatcc cgagggaatt tttatattca
     1861 tatcacctcc tagtattaag gagttgaaaa aacgcattat aaaaagaggc agtgacaccg
     1921 agagctcaat tgaacttagg atgaagagcg ctatagatga aattaaggct gcatctgagt
     1981 atgattatat tgtattaaat gatgacttaa atcaggctgc ccttaacctg cagtcgatag
     2041 ttttagctga aaggtgcaag gtatctagaa atacaaaaat tgtactaaag ttaagggagg
     2101 aatttggcaa tgatgcatcc gtcaattgac tcacttaccg aaaaatacga cagtaaatac
     2161 acaattgccg tagctgcagc aaagcgagct cgtcagttag tggagggtgc aaaagaactg
     2221 gttgatgtta aaacaaaaaa accggtatca ttagccttgt tcgagctaga cgctcaaaaa
     2281 attgtggttt acagaccaac aaccggaaaa aagtaattga ggtgtaatat gctacaaggt
     2341 aaattcgttg tattaggcgt taccggcagc atagcagcat ataagtcggt tgagctggtg
     2401 cggcttttaa aaaaagccgg agccaatgtc caagttatta tgacaaaatc tggctgtaaa
     2461 ttcataaagc ccctgacatt tcagg
//
LOCUS       DAONUO010000296         2418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_29539_length_2418_cov_3.701650, whole genome shotgun sequence.
ACCESSION   DAONUO010000296 DAONUO010000000
VERSION     DAONUO010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29539_length_2418_cov_3.701650"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PK768_09380"
     CDS             <1..704
                     /locus_tag="PK768_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA61222.1"
                     /translation="EEWKYQQMQKEFVANVSHELRTPLTTIKSYAETLLDGAMEEKEL
                     SYQFMNVINNEADRMTRLVNNLLELSRLDNRETKWDLKAMDSVAILNEVITKMRVNIQ
                     RKGQVLEVNLPEKSPEVFVDKDKIEQVFQNILSNANKYTPEGGKIIIGLDYTDSMVSI
                     SFKDTGIGIPNEDLPRIFERFYRVDKTRSREMGGTGLGLSIAQEIIRAHDGDIQIDSE
                     PGKGTCVTVCIPTIS"
     gene            841..1254
                     /locus_tag="PK768_09385"
     CDS             841..1254
                     /locus_tag="PK768_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61223.1"
                     /translation="MRRTLTAFLIAAILLTVFSPLAKADEVKNFIKLNEPKDNLMTSS
                     SRILLAGETVPNAKIVVLVNGRQEGKSLSVGAAGIFMTQVPISSKENIITVKASFPSG
                     GTETATRKVFLLEDESKLPELTSLIQTLKSFLILK"
     gene            1270..>2418
                     /gene="yycH"
                     /locus_tag="PK768_09390"
     CDS             1270..>2418
                     /gene="yycH"
                     /locus_tag="PK768_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HQA61224.1"
                     /translation="MSRDSILGWILTVLVVISLFLSFSIWSQVPGNFSAFKISHDDNK
                     VDPIQTVGPDKILVYFGNSFSTLLKPSAPLFEKSWSIARELLRAGSNLSPEPIGNISR
                     ENYKRTKGMEVFFPTPLPANFLKQLFNIEAGDFSSLDGKFINSFLLLDEGELSVYLVD
                     TEDLLYKIGKGSKTQDLNVIIRQIDESDPPLYANLTADVNIKVVGDVYVSLSPYELPV
                     YALKREQTSEEQIATKFFPDFSITRRIQERDGTVIYTDGQQGLRFYDDGSFEYNIPVS
                     RDARKTQGFYESFITAIDFIAGHGGFPADAYLASYEESSGPSGQTFVFKFRISANGFK
                     IINTDDFINITVEGGQVKNYYRNPSFSTKQIGILDLMTPVEALNTAVST"
BASE COUNT          733 a          494 c          543 g          648 t
ORIGIN      
        1 ccgaggagtg gaagtaccaa cagatgcaga aggagtttgt ggccaatgtt tcccacgaac
       61 tccgaacccc gctgacaacg ataaagagtt atgccgaaac actgctggac ggcgcgatgg
      121 aggaaaaaga gctctcctat caattcatga acgtgataaa caatgaggcc gatcgaatga
      181 cccgcctggt aaacaatttg ctggagttgt cccgccttga caaccgggag accaaatggg
      241 atctaaaagc catggattca gtggccatat tgaatgaagt tattacaaaa atgagagtca
      301 acattcagcg aaaagggcaa gtgctcgaag tcaatttgcc agaaaaatca cctgaagttt
      361 tcgttgataa ggataaaatc gagcaggtgt ttcagaacat cttgagcaat gccaataaat
      421 acacacctga aggcggaaaa atcattatag ggcttgatta taccgatagc atggtaagca
      481 tatcctttaa ggatacgggc atcggaatac ctaatgaaga cctgcccagg atttttgaaa
      541 ggttttacag ggtagataag accaggtcga gggaaatggg aggtactggc cttggcctat
      601 cgattgccca ggagataata cgggcccacg atggcgatat tcaaatagat agcgagccgg
      661 gcaaggggac ctgcgtaacc gtatgcattc ccacaatctc ttgagaccta taaagaggaa
      721 atgtaacttg tttttaaccg gcttgtaaca catctgtaac ataattgctt taaacttaag
      781 ttgaatatag gggaaataaa gccggtaata tgccttttat ttccagaaag ggagattatc
      841 atgaggagaa cattgacagc ttttttaatt gctgcgatac ttttaactgt tttctctcct
      901 ttagccaaag cagacgaggt aaaaaatttt atcaagttaa acgaacctaa agacaattta
      961 atgacctctt ccagcagaat attgctggcc ggcgagactg ttcccaatgc aaaaattgta
     1021 gtcttggtaa atggccgcca ggagggcaag tcactttctg tcggagcggc gggtattttc
     1081 atgacacagg tgcctatttc ctcaaaagag aatataatta cggtaaaggc ttcttttccc
     1141 tccggtggta cagagacagc tactcgcaag gtttttttac ttgaagatga gtcaaaatta
     1201 ccggaactaa cttccctgat acagacccta aaatctttcc tgattctcaa ataaaaggcg
     1261 gtgctgccat tgtcaaggga ttctatcttg ggatggattc ttactgtgct ggtagtcatc
     1321 agcctttttc tttcttttag tatatggtct caggttcccg gtaatttttc ggcttttaaa
     1381 ataagccacg atgacaataa agttgaccct atccaaacag taggtcctga caaaatattg
     1441 gtctactttg gcaactcttt tagcaccttg ctcaagccat cagcgccctt attcgaaaaa
     1501 agctggagca ttgcaaggga gctgctgagg gcaggctcaa atctaagccc ggagccgatt
     1561 ggaaatatct cccgggaaaa ttataagcgc acaaagggca tggaagtgtt ttttcccaca
     1621 ccacttccgg ctaatttttt aaagcagctt tttaatatag aagctggaga cttctcgtct
     1681 cttgacggta agtttatcaa ttctttctta ctgcttgatg agggagaact ctccgtttac
     1741 ttagttgaca ccgaggattt gctatataaa ataggaaagg gttcgaaaac acaggattta
     1801 aatgtgatca tcaggcaaat agatgaatcg gatccacccc tttatgccaa cttgacggct
     1861 gatgtgaata tcaaggttgt gggagatgtt tatgtttcac tttcacccta cgaactgccg
     1921 gtatatgctt tgaagcggga acagacttcg gaagaacaaa tcgcaaccaa gtttttcccc
     1981 gatttttcaa taaccaggag gatccaggag cgagacggca ccgtaatata caccgacggt
     2041 cagcaaggtc ttaggtttta cgacgatggt tccttcgagt acaacatacc ggtatcgaga
     2101 gatgcgagaa aaactcaggg cttttatgaa agttttatta cggctataga ctttattgca
     2161 ggccacggtg gattccccgc ggatgcttat cttgcatcat atgaagagtc aagtggtcct
     2221 tccggccaaa cattcgtttt taaatttagg atcagcgcaa acggctttaa aataataaat
     2281 accgatgact ttataaacat taccgtagag ggcggtcagg taaaaaatta ctacaggaac
     2341 ccatcttttt caacaaagca aataggcata ttagatttaa tgacgcctgt ggaagcttta
     2401 aatacagccg tatccacc
//
LOCUS       DAONUO010000297         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_29835_length_2399_cov_5.765358, whole genome shotgun sequence.
ACCESSION   DAONUO010000297 DAONUO010000000
VERSION     DAONUO010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29835_length_2399_cov_5.765358"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2254)
                     /locus_tag="PK768_09395"
     CDS             complement(<1..2254)
                     /locus_tag="PK768_09395"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HQA61225.1"
                     /translation="MAKLTLSQLENHLLKAADILRGKMDASEFKEYIFGMLFLKRLSD
                     QFEVEQQKVREKWEKEGFSKEEIEELVEDPGQYGGSFFVPKEARWRLKDDPNGGILNL
                     KEDVGNKLNIAMSKLEEYNSELGGVLKDIDFNAKKGNSRKTPDNKLIDLIAHFNKYRL
                     TNDDFVFPDLLGAAYEYMIKDFADSAGKKGGEFYTPSQVVRLLVNIVRPQEGNTVYDP
                     TCGSGGMLIQSKQYVAEQGQDDSNLALYGQDNNGTVWSICKMNMILHNIPDAHIENDD
                     TLENPVFRKDGFIMQFDRVIANPPFSQNYSRKKMEFPNRFKYGFCPETGKKADLMFVQ
                     HMIASLKKDGMMATIMPHGVLFRGGQEKEIRKGIVEDDIIEAIIGLPPNLFYGTGIPA
                     CVLVVNKSKPEFMKERILFINADAEYGEGRNQNYLRPEDIEKITYVFHNKIEQPKYSR
                     IVTLDEIRKEDYNLNIRRYVDNTPEPEIEDVRAHLIGGIPKREVALYKPVLEKYLIQE
                     SDIFCDRDSDYYDFRPELSTKEKIKETVNNHQGVKEAHQLIQMELEKFWEAASSKISE
                     LNSEKDIAEFRRSYIAILREKLSSFGILDIFQSAGVFVNWWEHSYLIREYKDYDEAND
                     KTITARETIQNKNALKTIVAQGWTSSLIPDEYIKNKYFSAEVEELERIEECIIEQENE
                     LSDYIDGIEIELIEEEGEEQEKTAKTVKAAIKQLIKDLKDTGTESALNQAAEWDKILK
                     EISYRKKKIKQ"
     gene            complement(2273..>2399)
                     /locus_tag="PK768_09400"
     CDS             complement(2273..>2399)
                     /locus_tag="PK768_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQA61226.1"
                     /translation="VRRVQVLCAQGRIQGAMRLGNIWAIPKNSPKPEDMRMKKEK"
BASE COUNT          604 a          586 c          404 g          805 t
ORIGIN      
        1 tctgtttgat tttctttttc ctgtaagaaa tttcttttaa aattttatcc cattcggctg
       61 cctggttaag ggcactttcg gttcctgtat cctttaggtc ttttatcaat tgttttatag
      121 ctgcttttac agtctttgct gttttctcct gttcttctcc ttcctcttct atcagctcta
      181 tctcaatacc gtcaatataa tctgaaagct cattctcttg ttctattatg cattcttcaa
      241 ttctttccag ctcctcaact tcggctgaaa aatacttgtt ctttatgtat tcgtcaggga
      301 tcaacgaaga agtccacccc tgggcaacaa tcgtttttaa ggcattcttg ttctgaattg
      361 tctcccgcgc cgtaatggtt ttgtcgttag cctcgtcata atccttatat tcacggatga
      421 ggtagctgtg ttcccaccag ttcacaaaaa ctccggcgct ttgaaatata tccaaaatcc
      481 caaaagaaga aagcttttcc ctcaagatgg caatataact ccttctgaat tcagcaatgt
      541 ctttttctga attaagttca ctgattttgg aagaagccgc ttcccaaaac ttctcaagtt
      601 ccatttggat aagctggtgg gcttctttca ccccctgatg gttattgaca gtctctttta
      661 tcttttcctt tgtgcttaat tccggcctga aatcatagta gtcggagtcc cggtcacaaa
      721 aaatatctga ttcctgaatc aaatattttt caagcactgg tttataaaga gccacttctc
      781 ttttaggtat accaccaatc aaatgcgccc tgacatcttc aatttccggt tcaggagtat
      841 tgtcaacata acgcctgatg ttgaggttat aatcttcttt tcggatttca tccaaagtta
      901 caattcgcga atacttgggc tgttcgatct tattgtggaa aacgtaggtt atcttctcaa
      961 tatcctccgg ccttaggtaa ttctgattac ggccttcacc gtattccgca tcggcattga
     1021 taaatagaat cctttccttc atgaattccg gcttgctttt attcactacc agcacacagg
     1081 caggaattcc tgtaccatag aaaagattcg gcggtaagcc aataatcgct tcaatgatat
     1141 cgtcctccac aatacctttg cgtatttctt tctcctgtcc gcccctaaag agcacgccat
     1201 ggggcatgat tgtcgccatc atcccatcct ttttcaagct ggctatcatg tgctgcacaa
     1261 acatcaaatc cgcctttttg cctgtttccg gacagaaacc atatttaaaa cggttgggaa
     1321 actccatttt cttgcggctg taattttgag agaagggtgg attagcaata acccggtcaa
     1381 actgcattat aaacccgtct tttctgaaaa caggattttc cagcgtatca tcattttcaa
     1441 tatgggcatc aggaatatta tgcaaaatca tgttcatttt gcatatcgac cacacagtgc
     1501 cattgttgtc ctgaccataa agggctaggt ttgagtcatc ttgtccctgc tccgcaacat
     1561 actgcttcga ctggataagc atgccaccgc ttccgcacgt agggtcatag accgtattcc
     1621 cttcttgagg cctgacaata tttacgagta aacgaactac ctgcgaaggc gtatagaatt
     1681 cgccgccctt tttgccagcg ctgtctgcga agtccttaat catatattca taagctgcac
     1741 ccaacaaatc cggaaaaaca aaatcatcat ttgttagcct atatttattg aaatgtgcta
     1801 tcaggtcaat cagcttgtta tccggagttt tacgcgaatt acccttttta gcattgaaat
     1861 caatatcctt aagcacacca ccgagctcgg agttatactc ttctagcttg ctcatggcaa
     1921 tgttcagctt attacccaca tcttctttca gattcagaat cccgccgttc ggatcatcct
     1981 ttaatctcca gcgagcttct ttgggcacaa aaaaactgcc gccatattga ccgggatctt
     2041 caacaagttc ttcaatttct tctttcgaaa acccttcttt ttcccacttc tctcgtactt
     2101 tttgctgttc tacctcaaat tggtcagaaa gacgctttaa aaaaagcata ccaaaaatat
     2161 attccttaaa ctcggaggca tccattttac cccgcagtat atcagctgcc ttgagtaaat
     2221 gattttcaag ttgagaaaga gtaagtttag ccattttttc accctctata atctattttt
     2281 ctttcttcat tcgcatatct tctggttttg gtgaattttt aggtattgcc catatattac
     2341 caagccgcat tgctccttga atccgtccct gcgcacataa aacctgcact cggcggacg
//
LOCUS       DAONUO010000298         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_29944_length_2393_cov_4.137725, whole genome shotgun sequence.
ACCESSION   DAONUO010000298 DAONUO010000000
VERSION     DAONUO010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29944_length_2393_cov_4.137725"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..804)
                     /locus_tag="PK768_09405"
     CDS             complement(343..804)
                     /locus_tag="PK768_09405"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HQA61227.1"
                     /translation="MLPMPTKYSLVAGSSEGSTVLNAFDGALLAAGAGNLNLLRVSSV
                     LPPGAVYDESLVIPPGNLVPTAYGAIECDVPETVISAAVGVGIPDDDTFGMIMEVSGK
                     FPKEVAEEKVREMLVEAFKMRNKALAEIKVKSAEHKVRKIGCAFAGVLLWY"
     gene            complement(878..1582)
                     /locus_tag="PK768_09410"
     CDS             complement(878..1582)
                     /locus_tag="PK768_09410"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQA61228.1"
                     /translation="MYEKQKIKVALLVFVIILIGISYNVFITRSIQALRFLNPGKTVV
                     IDPGHGTIDKGAVHEATGVAESPINLEVALKLKELLEAEKINVVLTRDNETLEMDTNR
                     KELERRINLAAKSKADILVSIHANKFPDPQYFGAQCFYNPQRPESKLLALLIQEELKN
                     IDSENIREALPQDLFILRESPMPSVLIEMGFLSNPKDRERLQDPAFQKMMAESIKSGI
                     LRYFSGDSPTRNPEYY"
     gene            complement(1589..2242)
                     /locus_tag="PK768_09415"
     CDS             complement(1589..2242)
                     /locus_tag="PK768_09415"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HQA61229.1"
                     /translation="MGENLIWVPVPIRTHLITQEDDIVEVVLRYTSSAAEPGDIIVIA
                     ESPVAISQGRAFLSSSVKSGLLARFLCKFPNKDGSLATPQAMQLAINEVGMLRVLMGA
                     VAAAFGKALGRSGDFYRVAGKELAKIDDIAGTLPPFDRYIVLGPKNPQKITDEIYKKT
                     GIATVIADINDIGCVDILAVSGKVSEKQLTEILKHNPFGNDDQQTPIVILKKTNKGG"
BASE COUNT          668 a          554 c          460 g          711 t
ORIGIN      
        1 aagggaaaaa ttagaaaaac ttatcccatt tgtgaaagca atgaaaagag ttgacaacaa
       61 agccaccata tacctatgtc aaaactttgc ctgcaaatca cccataagca gcatgaaagg
      121 cttagaagag attcttgaga aaacatatta aaattgacaa ctacaaacag cttaatttac
      181 cctacctgct ttgacattcg ttgccaagcg tgtgataggc tcatgtatcg atttagctcc
      241 cgctttgcta attaacgttc attgttttca caaatccaaa cggctaaaac tctcaaaata
      301 aataagaacc ctaaaagcgg gttcttgtct tttatttcct tatcaatacc acagtagcac
      361 tcctgcaaaa gcgcagccga tttttcttac cttgtgctct gctgacttta cttttatctc
      421 tgccagagcc ttatttcgca ttttgaaagc ttcaactagc atttctctta ccttttcttc
      481 agccacttcc ttaggaaatt tgccggaaac ttccatgatc atgccgaaag tatcatcatc
      541 cgggattccc actcctactg cggctgaaat aacggtttcc ggcacatcac attcaatagc
      601 cccatatgcc gtagggacta ggtttcccgg tggaataaca agactctcgt cataaacggc
      661 accgggcggc aaaacactgc tgacccgcaa gagatttaaa ttaccggctc ctgcggcaag
      721 caatgctcca tcaaaggcat tcaatactgt cgacccttct gaacttccgg ctaccaggct
      781 atatttagta ggcatcggca acataccaaa accccttttc agttaatatc atgatgtatc
      841 atatgaaaag aataccatgt atgtgcaata ttttcaatta gtaatattca ggattcctcg
      901 taggggaatc gccggaaaaa tatctaagga tgccactctt gatactttcc gccatcatct
      961 tctgaaaagc agggtcttgg agcctctccc tgtccttagg atttgataaa aaacccattt
     1021 cgattaagac agagggcatg ggactttctc ttaaaatgaa taaatcctgt gggagggctt
     1081 cccttatgtt ttctgaatca atgtttttca attcttcctg gatcagcaaa gccagcaact
     1141 tgctttcggg cctttggggg ttgtaaaaac actgggctcc gaaatactgc gggtcaggaa
     1201 atttgtttgc atgaatgcta accaggatat cagcctttga tttggcagcc aggttgattc
     1261 tcctttcaag ttccttgcga ttggtgtcca tctcgagagt ttcattgtcc cgagtcaaaa
     1321 ccacattgat tttttcggct tccaacaatt ccttgagctt taaggctact tccagattta
     1381 tggggctttc tgcaacacct gttgcttcat ggactgcacc cttatcaatg gtgccatggc
     1441 caggatcaat tactaccgtt ttaccaggat ttaaaaacct cagtgcctgt atagaccggg
     1501 ttataaagac attgtagcta atccctatga gaattatgac aaataccagc agggctactt
     1561 tgattttttg tttttcatac aagtcagttc acccaccttt attagttttt ttgaggatca
     1621 caataggtgt ttgttggtcg tcgttcccaa agggattatg cttgagtatt tctgttaatt
     1681 gtttctctga gactttgcct gaaacggcta atatatccac acatccaatg tcatttatgt
     1741 ccgcgatcac ggtagctata cctgtctttt tgtatatttc atcggtgatt ttttggggat
     1801 ttttcggccc taaaacaata tatctatcaa agggcggcag ggtccctgct atgtcatcga
     1861 ttttggccag ctcctttcct gcaactctgt aaaagtctcc ggagcggcct aaggcctttc
     1921 caaaggctgc agctacggct cccatcaaaa ctcttagcat gcccacttca ttaatggcca
     1981 gctgcattgc ctgcggtgtt gcgaggctac cgtctttatt cgggaacttg cataaaaaac
     2041 gggccagtaa accgcttttg accgaactgg aaagaaaagc tcggccctgg gaaatggcaa
     2101 cggggctttc cgcaatcaca attatgtctc caggttcggc agcggaagat gtgtacctca
     2161 gcacgacctc aacgatatca tcttcttgtg taatcaaatg ggtacgaatg ggtactggca
     2221 cccatatcaa gttttcgccc attataaaaa ccccccgcac taaatcttta tttttcccgc
     2281 catactttta gaagtaacaa aacatggtac agataaaaat agttggaata agataatgta
     2341 gaattgaaga aaggagcaac tttatgataa tcttagaaca acttttaaac gga
//
LOCUS       DAONUO010000299         2343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_30791_length_2343_cov_3.281906, whole genome shotgun sequence.
ACCESSION   DAONUO010000299 DAONUO010000000
VERSION     DAONUO010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30791_length_2343_cov_3.281906"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..288)
                     /locus_tag="PK768_09420"
     CDS             complement(13..288)
                     /locus_tag="PK768_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HQA61230.1"
                     /translation="MAELKRIVVSLPDNLLEKVDYFVALENKNRSELIRDAMKLYIRE
                     MEKIRIREQLKIGYIKMAELNIKFAEMGFCEDCKDFLSYESRLSESE"
     gene            complement(379..1560)
                     /gene="alr"
                     /locus_tag="PK768_09425"
     CDS             complement(379..1560)
                     /gene="alr"
                     /locus_tag="PK768_09425"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQA61231.1"
                     /translation="MLRDLANIRPTWVEINLDNLRHNLMEIRRIISPATKICAVVKAD
                     GYGHGAVEVAREALASGASYLAVAFLDEAMELRENGITAPILILGFTPEKQFDKIVEY
                     NISQTVYSIESAATLSEYAIKRSKKAKVHIKIDTGMSRIGFCPESCTTDDLTQLFSLE
                     GLEIEGIFTHFARADERDKSFTNQQFESFTKTVKAIEQEGFRIPIRHAANSAAILQYP
                     ETHLDMVRPGIILYGLAPSDEVPMSIIHLKPVMSFKTRVSHVKILPKGRAISYGGTYI
                     TQRRSVIATLPVGYADGFSRLLSSKTQVLIKGQRAPVVGRICMDQCMVDVTDVQGSVS
                     IGDEVVLMGDDGGDKITAEEIARLMGTINYELVCGISKRVPRVYISGGKIQKVGKPLV
                     K"
     gene            complement(1520..>2343)
                     /locus_tag="PK768_09430"
     CDS             complement(1520..>2343)
                     /locus_tag="PK768_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQA61232.1"
                     /translation="GSVGMTGAAALSATAAVRSGAGLVTIGIPESLNDILEVKVTEAM
                     TLPLPETPDRSISLAALHKALSFARMCDVAVLGPGISTNDETREFVKRFIANCTVPLL
                     LDADGLNTIAENPDILKDAKAPIVITPHPGEMARLLSISVSEVQADRIIAVKTAAEKF
                     ACTAILKGARTLIASSEGRLMINPTGNAGMATGGSGDVLSGMIGAFLARGMKACEAAA
                     AGVYLHGLAGDLAAMEKGQTCLAAADIIDFLPKALKMMKVHSDAKGPCQYSSYLG"
BASE COUNT          628 a          594 c          423 g          698 t
ORIGIN      
        1 ctaaccagca cattattcac tctccgaaag tcttgattcg tatgataaaa agtctttaca
       61 atcttcacaa aaccccattt ctgcaaattt gatatttagc tcagccattt tgatgtatcc
      121 gatttttaat tgttccctaa tccttatctt ttccatttct ctaatatata atttcatcgc
      181 atcccgaata agttcgctgc ggttcttgtt ttcaagagca acaaaataat ccactttttc
      241 taacagattg tcaggcaagc ttacaacaat acgctttaac tcagccacac cggcaccccc
      301 tagctttatt aaaacaaagc acaacacgaa ttatccatag gtcaatataa ttatgtcaaa
      361 tcaacatgaa aaatatacct attttaccaa tggctttccg accttttgga ttttcccacc
      421 acttatatat actctcggca cccttttaga tataccacat acaagttcgt aattgatggt
      481 gcccataagt ctggctattt cttcagcagt tattttatca ccaccatcat ctcccattag
      541 gacaacttca tctccgatgg acaccgatcc ttgtacgtct gttacgtcaa ccatgcactg
      601 atccatgcaa atgcgtccta ctacgggagc cctctgacct tttattaaaa cttgtgtctt
      661 agatgacaaa agtcttgaaa aaccgtcagc ataaccaacg ggcaatgtgg caatgacgct
      721 tcgtctttgt gtaatataag ttcctccgta acttatagcc cttccttttg gtaaaatttt
      781 cacatgagaa accctggttt taaaactcat aacgggtttt aaatgaatta tactcatcgg
      841 cacttcatct gatggagcta atccgtagag aataataccc ggtcgcacca tatccagatg
      901 tgtttcggga tactgcagga tcgccgcgct atttgccgca tgccttatgg gtatcctaaa
      961 accctcttgc tcaatggctt taaccgtctt cgtgaacgat tcaaattgct gatttgtaaa
     1021 gcttttatcc ctttcatcag cccgtgcaaa atgggtgaag atgccctcta tttcaaggcc
     1081 ttctaaggaa aaaagctgtg ttaaatcatc ggttgtacag ctttctgggc aaaagccaat
     1141 acggctcatg ccggtatcta tttttatatg aaccttagct tttttacttc gctttatagc
     1201 atattcagaa agtgtcgccg ccgactcaat actgtatacg gtctggctga tattgtactc
     1261 tactatttta tcaaattgct tttccggtgt aaaacccaaa ataaggatgg gagccgtgat
     1321 accattttct ctcagctcca tggcctcatc caaaaaagca acggcaaggt aagaagctcc
     1381 ggatgctaat gcttcccttg caacttccac agctccgtgt ccgtacccat cggctttcac
     1441 tacggcacat atcttggtag ctggagaaat tatccttcta atttccatta agttgtgcct
     1501 caaattgtct aaattaatct caacccaagt aggacgaata ttggcaaggt cccttagcat
     1561 cgctatgcac cttcatcatt ttaagcgctt taggcagaaa gtcaattata tccgcagcag
     1621 caagacaagt ttgacccttt tccattgctg ctaaatcgcc tgctaagccg tgcaggtata
     1681 cccctgctgc tgcagcttca caggctttca ttcctcgagc caaaaaggct ccaatcatgc
     1741 ctgatagcac atcaccgctg ccgccagttg ccatgcccgc attgcccgtt ggattgatca
     1801 taagccttcc ttccgaacta gcaattaaag tccttgcacc ctttaaaatt gcagtgcaag
     1861 caaacttttc ggcagcagtt tttaccgcta ttatcctatc agcctggact tccgaaacag
     1921 atatcgatag aagccttgcc atctctccgg ggtggggagt tatgacaatt ggggccttgg
     1981 catcttttaa aatatccggg ttttctgcaa tggtgttaag cccatccgca tccaatagca
     2041 aaggtacagt acagtttgca ataaatcttt ttacaaactc ccttgtctca tcattagtgg
     2101 aaatgcccgg acctaaaact gcgacatcgc acatccttgc aaaacttaaa gctttatgga
     2161 gggcagcaag gctaatgctg cgatcaggtg tttcgggaag gggcagggtc atagcttccg
     2221 tcactttaac ttccaggatg tcatttaggc tttcgggaat gcctattgtt acaaggccgg
     2281 caccgcttct caccgctgca gttgctgaca gggccgccgc ccccgtcatg ccaacggaac
     2341 cag
//
LOCUS       DAONUO010000300         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_30883_length_2337_cov_4.783085, whole genome shotgun sequence.
ACCESSION   DAONUO010000300 DAONUO010000000
VERSION     DAONUO010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30883_length_2337_cov_4.783085"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..499
                     /locus_tag="PK768_09435"
     CDS             95..499
                     /locus_tag="PK768_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA61233.1"
                     /translation="MIARRIPTILPSMTFEESLELTKIYSIAGLLSDHDGLIEKRPFR
                     APHHTISAVGMVGGGRIPKPGEITLAHNGVLFLDELPEFPRDVLELLRQPLEDEKVTI
                     ARANATITYPSKFMLVAAMNPCPCVTKIPSHP"
     gene            complement(438..2108)
                     /locus_tag="PK768_09440"
     CDS             complement(438..2108)
                     /locus_tag="PK768_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQA61234.1"
                     /translation="MVVAAIYGRRSVDDDPSLSVDNQINRAISLCKAKNWGYIIYRDD
                     GVSGKDTDREGFKQMMRDAKKGKFQYLVCYKLDRVSRSVADFSSLLNELQELGVEFIS
                     LTENFDTSTPMGRAMMYIAAVFAQLERETIAQRVKDNMIDMAKQGYFTGGPVNFGFDL
                     VTEEMIIRGKVKEVSRLVENEQEKQHVIDFYHWYNEPQGSVRNNCIIANKSGIRTKSG
                     KLWNPNQMARLLSNALYCIADEDAYNYFKNETNVKIVSDKKDFNGINGLMYYNRRIPH
                     KKTTRLRDENEWILAVGQHKGFIPGKIWAETQRKLKKNASTPPRAGTATKGLLAYLVK
                     CGHCGGSMSYYPSWKTVDGIEYGYYRCRRRETQGKHLCDQPNINSYVLETTVINHIKK
                     SLSDISFVERAIKQAKKEMLSQKAPSEKELRRIEKEIEANNTEMKNLIIALGRKTIPA
                     ELIENRIKELQEQIAELKQEMMEIKSTIDFIGSVDDINIVYHFINEFNNSFDEMDFED
                     KRLFLRYLIKEIVVTDYEVHIYMFFLPGIKDDLVFSSRTGKDSSPQLT"
BASE COUNT          631 a          502 c          390 g          814 t
ORIGIN      
        1 ggtgctatac ctaaagaacc atgcagaact gagcgaatag cataaaacca tttataacat
       61 tatcatgata ggttctccgg gatcaggcaa gactatgata gcaaggcgaa taccaacgat
      121 tttgccttcg atgacttttg aagaatccct agaattgacg aaaatatata gcattgcagg
      181 tttactttct gaccacgacg gtctgattga gaaaaggcct tttagggccc cgcatcatac
      241 gatttcagct gtaggaatgg taggcggtgg tcgcattccc aaacccggag agattacact
      301 agcacataac ggagtgcttt ttttggatga gctacctgag tttcccagag atgtgctgga
      361 acttttgcgc cagcctctag aggacgaaaa agtaactata gctcgggcaa atgcaactat
      421 tacctatcct tcaaaattta tgttagttgc ggcgatgaat ccttgcccgt gcgtgacgaa
      481 aataccaagt catccttaat tcctggtaaa aagaacatat aaatatgtac ttcataatct
      541 gttacaacaa tttcttttat caagtatctt aaaaagagtc ttttgtcctc aaaatccatt
      601 tcatcaaatg agttattaaa ctcattgatg aagtgataga cgatatttat gtcatctaca
      661 ctgccaataa agtctattgt actctttatt tccatcattt cctgctttag ctctgcaatt
      721 tgttcttgca gttctttgat tcgattttcg atcagttccg ctggaattgt ttttctccct
      781 aaagctatta ttaaattctt catttctgta ttgtttgcct caatttcttt ttcaattctt
      841 cttagttctt tttcagatgg tgctttttga gatagcattt ctttctttgc ctgctttatt
      901 gctctctcaa caaaactaat gtctgaaagc gattttttga tatgatttat cacagtagtt
      961 tcaaggacat aactatttat attaggctga tcgcataaat gcttgccctg ggtttccctt
     1021 ctcctacatc tgtaatatcc gtattcaatg ccatctacgg ttttccagct aggataatat
     1081 gacatagagc caccacaatg accgcattta acaagatatg ctaaaagacc cttggtggca
     1141 gttccggccc ttgggggagt agatgcgttc ttttttaatt tgcgttgagt ttctgcccaa
     1201 attttgccgg gtataaatcc tttgtgttga ccgacggcta atatccattc attttcatct
     1261 ctaagccttg tggttttttt atgaggtatc cggcgattgt aatacatcag cccgtttatc
     1321 ccattgaaat cttttttgtc gcttacgatt tttacatttg tttcgttttt aaaatagtta
     1381 tatgcatctt cgtctgcaat acaatacaaa gcattagaaa gcagtctggc catttgattt
     1441 ggattccaaa gcttgccgct tttcgttctt atcccagatt tgttggctat tatgcaattg
     1501 tttctcacag agccctgcgg ttcattgtac cagtggtaaa agtcaattac gtgctgcttt
     1561 tcttgttcat tttcaactaa cctggatact tcctttacct ttcctcgtat gatcatctcc
     1621 tcggtaacca agtcaaagcc aaaatttact ggtccaccag taaaataacc ttgtttcgcc
     1681 atgtcaatca tattatcttt aactctttgt gctatcgtct ccctttccag ttgagcaaat
     1741 actgccgcta tatacatcat ggctctgccc ataggagttg atgtatcaaa attttccgta
     1801 agggagatga attctactcc aagttcttgt agttcattta ataggcttga aaaatcagca
     1861 acgctccttg aaaccctgtc caatttataa caaaccaaat attgaaactt tcctttcttg
     1921 gcatctctca tcatttgttt gaaaccttcc ctatccgtat cctttcccga aaccccatca
     1981 tccctgtata ttatgtagcc ccagtttttt gctttgcata ggctaattgc acgatttatt
     2041 tggttgtcaa cgcttaaact agggtcatca tctacacttc ttcgcccata aattgcggct
     2101 acaaccattt gtaagacctc ctttacttcc ttctattaaa gtataaaaaa agacgcctta
     2161 aaaggcaaat ttgggcgaac gaggatagat agttttattg taatactaaa accaatcctt
     2221 caatgaaatt gtaacatcta ggttgttatc aaatttctta aagcaacatt ccttatgcgg
     2281 cagtttttta gttacacacc ctctcctggc atttatacta acacactcaa attggtc
//
LOCUS       DAONUO010000301         2328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_31026_length_2328_cov_3.790145, whole genome shotgun sequence.
ACCESSION   DAONUO010000301 DAONUO010000000
VERSION     DAONUO010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31026_length_2328_cov_3.790145"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /gene="pheT"
                     /locus_tag="PK768_09445"
     CDS             <1..1407
                     /gene="pheT"
                     /locus_tag="PK768_09445"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQA61235.1"
                     /translation="EITPSTTRILLESANFLGSSIRQTSRQLGLRSESSMRFEKGIDP
                     NLCLKAADRACELIEELGAGKVLKGYVDVFPGTCYPKAVRFSPDKINKVLGTDISSDE
                     MVDILERLEIKVNSYEDVFEAIVPTFRMDITLEADIVEEIARIYGYDRLPITLPEGNV
                     THGRLNEKQKFLDEIREFMVYKGYSEIYTFSFVSPKVFDKINAPEDSNLRQAISLLNP
                     LGEDHSIMRTTLIPNMLDVISFNLNRKLDELELFEVGAAYLPEKLPLEELPHENKRIS
                     IGTCGDAVDFYVLKQVIESLFYRLQIQNYKFEQSRHFAFHTGRCARITIDDEVIGYAG
                     EIHPDVLENYEISKKVYLAELDLDIILDKASRKIEFKPLPKFPSSDRDLAIVVDEKVL
                     AADVITVIREIGGKLLEEVELFDVYQGSQIESGYKSMAFSLVFRAEDRTLTDKEVNDI
                     MERITAGLEEKFDASLRK"
     gene            1519..1788
                     /locus_tag="PK768_09450"
     CDS             1519..1788
                     /locus_tag="PK768_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQA61236.1"
                     /translation="MANEAKQVNRVKVNINGEDYYIKGTVPEEYIKRVAKYVDKKMYE
                     LESKHPNLSRSKLAVLTAINITDELFKLRQEYEEFLITFDGSTKE"
     gene            1795..>2328
                     /locus_tag="PK768_09455"
     CDS             1795..>2328
                     /locus_tag="PK768_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQA61237.1"
                     /translation="MKNFEVAFIFYDIADILEIKGENFFKIRAYRKAAHTIENLPMDV
                     KELAGESRLQEIEGIGKALEEKIREIINTGTCRYYEELKKDFPRGLVEMLKIPGLGAK
                     KIKVIYDNLGISSIEELEKAARAHKIRELPGMGVKTEQSILKGIQTLKGQGDRVQLAI
                     ALPVANRIMEILSGMKEV"
BASE COUNT          751 a          447 c          578 g          552 t
ORIGIN      
        1 gaaatcactc ccagcactac tcgcattctc ctggaatccg ccaatttcct gggttccagt
       61 ataaggcaga cctcgaggca gctgggcctt cgttccgagt cttcaatgag atttgaaaag
      121 ggcatcgacc ccaacctgtg cctgaaagct gccgataggg cgtgcgaact catcgaagag
      181 ctgggtgcag gaaaagtctt aaagggctat gtagacgtat ttccgggcac atgctatcca
      241 aaggctgtca ggtttagccc ggataagata aacaaggtgc tgggcaccga tattagctcc
      301 gatgaaatgg tggacatact cgagcgtttg gagattaagg ttaacagtta cgaggatgtt
      361 tttgaagcga ttgttcccac atttaggatg gacataaccc tggaggcgga tatcgtcgag
      421 gaaatcgccc gaatatacgg atacgacaga ttgcctataa cactgcctga agggaatgtc
      481 acccatggca gactgaatga aaagcaaaag ttcctggatg aaataaggga attcatggta
      541 tacaagggct attccgaaat atataccttt tcctttgtaa gtccaaaggt cttcgataaa
      601 ataaatgctc cggaagacag taacctgaga caagccataa gccttttgaa ccccctggga
      661 gaagaccaca gcatcatgag gaccacccta atacccaata tgctggatgt aattagcttt
      721 aatttaaacc gcaagcttga cgaactggag ctctttgaag ttggggctgc ttacctgccc
      781 gaaaaattgc cccttgagga gcttccccat gagaacaagc gaatttcaat tggcacatgc
      841 ggggatgcag ttgattttta tgttctaaaa caagtgatcg aaagcctgtt ttacaggttg
      901 caaatacaga actataagtt cgagcagtcg cggcattttg ccttccatac cggacgctgc
      961 gcccgcatca caatagatga cgaagtgata ggatatgccg gtgaaatcca ccccgatgta
     1021 ctggaaaact atgaaataag caaaaaggtc tacctggcag aacttgacct ggatatcata
     1081 ttagacaagg cttcaaggaa aatagaattc aaaccactgc ccaaattccc atcttcagat
     1141 agggacctgg caatagtcgt cgacgaaaaa gtattagcag ctgacgtgat aactgtcata
     1201 agggaaattg gtggaaagct gcttgaagaa gtagagctct tcgatgttta ccagggcagt
     1261 cagattgaaa gtggatacaa gagcatggcc ttttcccttg tatttagggc cgaagacagg
     1321 acccttaccg acaaagaggt caatgatatt atggaaagga taacggctgg ccttgaggaa
     1381 aaattcgacg ccagtttgag gaaataaagg attttgtaga tatttgaaga ataattacaa
     1441 taaatgttaa tagtttaaga agcggcattt gccgcttctt aaaatcttac gggaatatga
     1501 ataactgggg tggtttgcat ggccaatgag gcaaaacagg taaacagggt taaagttaat
     1561 attaatggag aagattatta tataaaggga acggtacctg aagagtacat caagcgagtt
     1621 gccaaatacg tggacaaaaa aatgtatgaa ttggagagca aacatcctaa cttgagccga
     1681 agcaaattgg cggtgctgac agcaattaac atcaccgatg agcttttcaa attaagacag
     1741 gagtacgaag agtttttgat tacatttgat ggcagtacaa aggaatgaat attgatgaag
     1801 aattttgaag tagcctttat cttctacgac attgccgaca ttttagaaat caagggagaa
     1861 aattttttta aaataagggc atatcggaaa gctgcccaca caatagaaaa tcttcccatg
     1921 gatgtgaaag aactggccgg ggagtcacgc ctgcaggaga tagagggcat aggaaaggct
     1981 ttagaagaga aaatccggga aattatcaat accggaacct gcaggtacta tgaggaatta
     2041 aaaaaggatt tccccagggg ccttgttgag atgctgaaaa tacccggcct gggcgctaag
     2101 aagataaaag taatttacga caatctaggc atttcctcga ttgaagaatt ggaaaaggca
     2161 gcaagagccc ataagatcag ggaattgccg ggcatgggcg taaagaccga gcagtcgatc
     2221 ttaaagggca tacagacctt aaaaggtcag ggggataggg tgcagctggc catcgccttg
     2281 cctgttgcaa acaggattat ggagattctt tccggcatga aggaagta
//
LOCUS       DAONUO010000302         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_31121_length_2322_cov_3.133216, whole genome shotgun sequence.
ACCESSION   DAONUO010000302 DAONUO010000000
VERSION     DAONUO010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31121_length_2322_cov_3.133216"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PK768_09460"
     CDS             <1..687
                     /locus_tag="PK768_09460"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQA61238.1"
                     /translation="VPAGKIRLTLMVIGIIGILAISIYAADNFILPLSFALLILSMVP
                     FYMKFEKKELNAEEMVLMAMLSALAAVGRVPFAALPSIQATSFIVIMAALSFGREVGF
                     LVGNTAALISNVFLGQGPWTPWQMLGWGMMGFTAALLKDARLLKSKFQLCIFGFVWGF
                     MFGWMMNIWVLLFFQLSDISWPLILGSCIASFNFDLYHALCNVIFILLFYDRFKRILS
                     RTKEKYGLLK"
     gene            823..1242
                     /locus_tag="PK768_09465"
     CDS             823..1242
                     /locus_tag="PK768_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HQA61239.1"
                     /translation="MLIEKIEEYYKERDLNCAETMLYAANDVYGLELDKKALKMAAGF
                     GGGMYIRSVCGALAGGVMVISRLFIKDRAHASPELRGLVEEFLHRYCNKMGDINCSYL
                     KEHYHNEEVRCLNVIKEAGIILDDIVKRELKSSKDID"
     gene            complement(1264..1758)
                     /locus_tag="PK768_09470"
     CDS             complement(1264..1758)
                     /locus_tag="PK768_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQA61240.1"
                     /translation="MKAKEIVTSALLTALSLLIPITFGGYLKIYIPPFSATLASHVPS
                     MIAMLISPTAAVMVGLGSTLGFLLIMGPVVAARAFVHVFFGLAGALLIKKGLSFQKAL
                     IYVAPIHALGEALIVLPLGFNMHTAFVVVGIGTLLHHFIDSAVSVGLVKALSAGLSLS
                     GKKV"
     gene            complement(1949..>2322)
                     /locus_tag="PK768_09475"
     CDS             complement(1949..>2322)
                     /locus_tag="PK768_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HQA61241.1"
                     /translation="SGKDIISTSQGYLMKSPLPVCTKQIAVCHKEENTREELLTFIEC
                     GCKVIDVIVEHPLYGELHGLLMLSNSKDVDNFIQAIKKHKAKLLSQLTNGVHLHTVEA
                     INFDCINKAERLLKEKGMLLE"
BASE COUNT          701 a          424 c          511 g          686 t
ORIGIN      
        1 gtacctgctg gcaaaatccg cctcacactg atggtgatag gcataatagg gattcttgcc
       61 atttcaatat atgcggcgga caattttatc ttacccttga gctttgcatt acttatattg
      121 tcgatggttc ctttttacat gaagtttgag aaaaaagaac taaatgcgga ggaaatggta
      181 ttgatggcca tgctatctgc ccttgcagcg gtgggtaggg taccctttgc ggcccttccc
      241 agcatacaag ctacctcttt catagtaatt atggccgccc tatccttcgg aagggaagtg
      301 ggatttttag ttggaaatac cgctgcattg atttcgaatg ttttcttggg acaggggcct
      361 tggacacctt ggcagatgct gggctgggga atgatgggct ttactgcggc tttgctcaaa
      421 gatgctcgtt tattaaaatc caaattccag ctctgcatct ttggcttcgt gtggggcttt
      481 atgtttggct ggatgatgaa catctgggta ttgctgtttt tccagcttag cgacatttca
      541 tggccattga ttttggggtc ctgcattgca agctttaatt tcgacctgta tcatgctctt
      601 tgcaacgtta tttttattct gcttttttat gaccgcttca aaagaatatt aagtaggaca
      661 aaggaaaagt atgggctttt gaaataacat gatatctcct aaaaacaaca attgaaattg
      721 tttgtatggt gtaataagat taagaaaaat aatatactat ttatcggaag tattcaaaag
      781 gaaaatcaag ggcaaattta atacaaagag aggagatatc acatgcttat tgagaaaata
      841 gaagaatact ataaagagcg tgatttaaac tgtgccgaga ccatgcttta tgcggcaaat
      901 gacgtatatg gactggaact agataaaaag gcccttaaga tggcagccgg ctttggcggg
      961 ggaatgtaca tccgcagtgt atgcggtgcc cttgcgggag gcgtaatggt gattagccgc
     1021 ctttttataa aggacagggc ccatgcaagc ccggaattaa ggggattggt agaggagttt
     1081 ctgcacagat actgcaacaa gatgggcgac ataaattgtt cctacttaaa agagcattac
     1141 cataacgaag aagtcaggtg cctcaatgtc ataaaagaag ccggaattat ccttgatgat
     1201 atagttaaaa gagaattaaa aagctcaaag gacattgatt aagcctttga gcagattaga
     1261 aagctatact tttttgcctg ataaagacaa acctgctgaa agtgctttta ccaggccgac
     1321 agaaacagcg ctgtcgataa aatgatgcag tagagtgccg atacccacca cgacaaaagc
     1381 cgtgtgcata ttaaaaccca ggggaagcac aatcaaagcc tctcctaagg cgtgaattgg
     1441 ggcaacgtat attaaggctt tttgaaaaga aaggcctttt tttattagta aggctccggc
     1501 caagccgaaa aacacgtgga caaaagccct agctgccaca acaggtccca taatcaataa
     1561 aaagcccaga gtagaaccca aacccaccat caccgccgcc gtagggctga ttagcattgc
     1621 tatcatggat ggcacatggg atgctagagt tgccgagaat ggaggtatat atattttaag
     1681 gtagcctcca aaagtaatag ggattagcaa tgacagggct gtgagtaggg cacttgtgac
     1741 aatttctttc gctttcatga cattagtcct ttcctatttt ttataacata aaataataaa
     1801 ggttttttca gttattgaaa aataaaaatt ccgattgctg ttgaattgcc atacaatagt
     1861 aattgtaaca agagctttaa aagatttgct tgttcatgac gattatactg tatatacacc
     1921 tgactagtca actggttaca aaaaaacatt attctaatag catacctttt tctttcagta
     1981 atctttcagc tttgtttata cagtcgaaat ttatagcttc aacagtgtga agatggacac
     2041 cgtttgttag ctgtgagagc agttttgcct tatgtttttt gatagcttgg ataaaattat
     2101 ctacgtcttt agagtttgaa agcattaaca aaccgtgaag ttcaccgtac aaaggatgtt
     2161 ctactatgac atctataacc ttgcagccac attcaataaa agttaatagc tcttcacggg
     2221 tattttcttc tttgtgacat actgcgattt gcttagtgca aacaggtagg gggcttttca
     2281 ttaagtaccc ttgggaagtt gaaataatat ctttcccgga ag
//
LOCUS       DAONUO010000303         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_31582_length_2296_cov_3.735386, whole genome shotgun sequence.
ACCESSION   DAONUO010000303 DAONUO010000000
VERSION     DAONUO010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31582_length_2296_cov_3.735386"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK768_09480"
     CDS             <1..192
                     /locus_tag="PK768_09480"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HQA61242.1"
                     /translation="LRLLKGLSKDAFKRRFNREITQVYESAIDKLKKQGLLQENSEYF
                     RLTAPGLDFANAVFMEFLP"
     gene            278..1312
                     /gene="hrcA"
                     /locus_tag="PK768_09485"
     CDS             278..1312
                     /gene="hrcA"
                     /locus_tag="PK768_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQA61243.1"
                     /translation="MMLDERKIKILAAVVHDYIATAEPIGSRTIARKYRIGISPATIR
                     NEMADLEEQGYLHQPHTSAGRVPSHKGYRYYVDFLMPIKKLSAAEQALIRSIFNRRVN
                     ELEELVKEAARVISNLTSYTTLTLGPQLKSSHLKYIQVNRIDETRGLLLMVTNYGTVS
                     HNIIDMPPTISDSDLERITNILNDNLSGKTISEINSDIMETIKTEMIEYDEILNILLE
                     ILIENLEEDAESGNVVSTGSSKMLDYPEFRDVEKAKNFFALLEQHDLITKALKSSSKP
                     NAINVTIGVENPLTELQDFSIVTSNIIVDGKDLGVFGILGPTRMEYSKVVSILEQVTN
                     YLVKTLSTML"
     gene            1334..>2296
                     /locus_tag="PK768_09490"
     CDS             1334..>2296
                     /locus_tag="PK768_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HQA61244.1"
                     /translation="MSLKDNTQEVDERLAALITNSNAIRAVAAAVEGTIGPKGLDIML
                     VDRFGEVTITNDGVTILKQMDVNHPAAKILINIAKAQQEEVGDGTTTATLMAGAMVAE
                     GVAQILRGVPVARVIEGIKIGINKAQEILNANVIPVNGIDDPNLKNVALIAGRENQDI
                     AELVTDAAKLIGEEKLKDKNFKFKDIIISRAGAENEVFLGLIIDKEKLNNQMPEELTD
                     VKILLIDDALEPEEVAPEALRTEAGFEKYLALKEEFKENLKKIIDLGVNLVLVDKCIN
                     DEAEEILTDAGIIALDRVSRKDMERVSEHSGARIMKRLGLKKPVE"
BASE COUNT          796 a          420 c          526 g          554 t
ORIGIN      
        1 cttaggctgc taaaaggtct gagcaaagac gcctttaaac ggcgctttaa tcgtgaaatc
       61 acacaggttt acgaaagtgc catagataag ctgaagaaac aggggttatt acaagaaaac
      121 tcggaatatt tcagattaac cgctccagga ttggattttg ccaatgccgt atttatggaa
      181 tttttgcctt aaattcttga caaaaaacac cccaagtgat attttttaag tagatttagc
      241 actctaagct tgagagtgct aaagagaggt gtttaaaatg atgctagacg agaggaaaat
      301 taaaatatta gcagcggtag tccacgatta tatagccact gccgaaccca tcggctccag
      361 gacaattgcc cgaaagtacc gcataggtat cagcccggcc acaataagaa atgaaatggc
      421 tgatttagaa gaacagggat atctgcacca gccccataca tcagcaggcc gcgtaccttc
      481 ccacaaaggc tatagatatt atgtggattt tttaatgccg attaaaaagt taagtgctgc
      541 agagcaggcg ttgattcgaa gcatctttaa tagacgagtt aatgaactgg aggagctagt
      601 aaaggaagcc gccagggtaa tatcaaatct gaccagttac accacattaa ccctgggccc
      661 gcaactaaaa agcagtcatt taaaatatat tcaggtgaac cgcatagacg agactagagg
      721 acttttgttg atggttacaa attacggcac cgttagccat aacattatag atatgcctcc
      781 taccataagt gattcagacc ttgaaaggat aacaaacata ttaaacgaca acctcagcgg
      841 aaagacgatt tcggaaatta acagcgacat catggaaact attaagaccg aaatgataga
      901 atacgacgag attctcaaca tactgttgga gatattgatt gagaacctgg aggaagatgc
      961 cgaaagcggc aacgtggttt ctacaggcag ctccaagatg ctggattatc ctgaatttcg
     1021 agatgttgaa aaagccaaaa acttttttgc actgctggaa cagcacgact tgatcacaaa
     1081 ggcactaaaa agttcatcga aacccaatgc gattaatgtt actattggag tcgaaaaccc
     1141 tctgactgaa ttgcaagatt ttagcattgt gacttcaaat attattgttg acgggaagga
     1201 tttaggcgtt tttggaatct tgggcccaac ccggatggag tattccaagg tcgtaagcat
     1261 acttgagcag gtgacaaatt atctggtaaa aaccctatca actatgctgt aataaaaaga
     1321 ggaggtatct actttgtctt taaaggataa tacccaagaa gttgacgaaa gattggccgc
     1381 tctaataaca aactcaaatg caataagggc tgtagcagcg gcggttgaag gaaccatcgg
     1441 gcccaaaggc ctcgatatca tgttggtaga ccgttttgga gaagtgacaa taacaaatga
     1501 cggagttacc atactaaagc aaatggatgt gaatcatcct gctgcgaaaa tccttataaa
     1561 catagcaaag gctcaacagg aagaagtagg agatggcact accacagcta cactcatggc
     1621 aggggccatg gtggccgaag gcgttgctca aattttaagg ggagttcctg ttgcccgagt
     1681 aatagaggga ataaaaattg gcataaacaa ggcacaggaa attttaaatg ccaatgtaat
     1741 ccccgtaaat ggaatagatg atcccaactt aaaaaatgtt gccctaattg caggcagaga
     1801 aaatcaggat attgccgagc tggtcacgga cgctgctaaa ctcatcgggg aagaaaaact
     1861 taaagataaa aattttaaat ttaaggacat cataatatct agggcaggag cggaaaatga
     1921 ggtcttccta ggccttatca ttgataaaga gaagctaaac aatcagatgc cagaggaatt
     1981 aactgacgta aagattttat taatagatga tgccttggaa ccagaagaag ttgcacccga
     2041 agctttgagg accgaagccg gttttgaaaa gtaccttgca ttgaaagaag agttcaaaga
     2101 gaatctcaaa aagatcatcg atttaggtgt caatttggtt ttggttgaca aatgcattaa
     2161 cgatgaagca gaagaaatat tgacggatgc aggcattatt gcattggata gagtttcaag
     2221 aaaggatatg gaaagggttt cggaacacag cggggcacgt attatgaaga gattaggcct
     2281 caaaaaaccc gtggaa
//
LOCUS       DAONUO010000304         2289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_31705_length_2289_cov_3.265891, whole genome shotgun sequence.
ACCESSION   DAONUO010000304 DAONUO010000000
VERSION     DAONUO010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31705_length_2289_cov_3.265891"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PK768_09495"
     CDS             <1..613
                     /locus_tag="PK768_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-mannonate epimerase"
                     /protein_id="HQA61245.1"
                     /translation="VISQVQGENLISGLLIGRERKVFEEAVKLSQEKNITLLEKPLEK
                     AVVFLDPEEFKSTWVGNKAIYRTRMAMADNGHLIIIAPGVRQFGEDEDRDILIRKYGY
                     VGRDEILSLTKNNEDLQNNLSTAAHLIHGSPDGRFKITYATDKLTKEDIEGVAFEYMP
                     LEDALKAYDINVLKDGFNTLENGEEVFYVSNPALGLWADKSKF"
     gene            641..2053
                     /gene="gndA"
                     /locus_tag="PK768_09500"
     CDS             641..2053
                     /gene="gndA"
                     /locus_tag="PK768_09500"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HQA61246.1"
                     /translation="MANSDIALIGLAVMGQNLAINIARNGYKISVYNRTAEKTKEFLE
                     SSAKGYGNITGAYSLEELVFTLKRPRKIMLMVKAGAPVDAMIAQLEPLLEKGDIIIDG
                     GNSYFKDTIRRAKELDKRGIHFMGVGVSGGEEGALWGPSIMPGGPKEAWKEISQIFID
                     ISAKTPDKVPCCDYIGPDGAGHFVKMTHNGIEYGDMQLISEAYFLMKNLLGMSALEMQ
                     EVFAEWNKGELNSYLIEITADILGKVDEETGEPLVDLILDKAGQKGTGKWTSQEALDL
                     GTPAPTIAEAVFARYMSAIKDERIKASQELKGPRPAFEGDKGQFIEDIKRALYASKIC
                     SYAQGFALMAEASKAYNWNLKLGNIALLWRAGCIIRAQFLDKIKEAYETNPKLQNLLL
                     APYFQQSIEDCQEAWRKVVSTAALKGVPIPSFSSALAYYDSYRTHTLPANLIQAQRDY
                     FGAHTYERVDREGIFHTIWV"
BASE COUNT          716 a          472 c          569 g          532 t
ORIGIN      
        1 cgttatatcc caggtgcagg gtgaaaacct catatcaggc cttttgatag gaagggaaag
       61 aaaggtcttt gaagaggcag taaaactcag ccaggaaaaa aacataacat tattagaaaa
      121 acccttagaa aaggctgtag tgttcctcga tcccgaagaa tttaagagca cctgggtggg
      181 caataaggcc atatacagga ccaggatggc catggctgac aacggccacc tcatcatcat
      241 tgctcccggt gtcaggcaat ttggagaaga cgaagatagg gacatcttaa ttagaaaata
      301 cggttacgta ggtagggatg aaatcctttc ccttactaaa aataacgaag acctccaaaa
      361 caacctatca acagccgccc atttaatcca cggttcccct gatggcagat ttaagataac
      421 ttatgctaca gacaaactga caaaagagga tatcgagggt gtggcttttg aatacatgcc
      481 ccttgaagat gccctgaagg catatgatat aaatgtgcta aaggacggat ttaacacttt
      541 agaaaacggt gaagaagttt tttacgtcag caatccggca cttggcttat gggctgataa
      601 aagcaaattt taatagctat tatgcagaaa ggatgatagc atggcaaact ccgatatagc
      661 cctaattggc ctcgcagtta tgggtcaaaa ccttgcgatt aatatagcaa gaaacggcta
      721 taaaatttcg gtctacaaca ggacggccga aaagaccaaa gaattcctag agagctcagc
      781 aaaagggtac ggcaacatta caggggcata ttccctggaa gaacttgttt tcaccctaaa
      841 aagaccccgt aaaataatgc tcatggtaaa agcaggtgcc ccggtggatg ctatgattgc
      901 ccaacttgag cctttgttgg aaaaagggga tatcatcatt gatggcggca actcctattt
      961 taaggatacg ataagaaggg cgaaggaact tgataaaagg ggaatacact ttatgggagt
     1021 gggggtttca ggcggagaag aaggagctct ttgggggccc agcatcatgc cgggtggccc
     1081 gaaagaggca tggaaggaaa tcagtcagat ttttattgac atttctgcaa agactcccga
     1141 taaagtgccc tgctgcgatt atataggtcc cgatggggcc ggccattttg taaaaatgac
     1201 acataacggc atagaatacg gcgacatgca gctaatcagc gaagcatatt tcctcatgaa
     1261 aaacctcctt ggaatgtccg cccttgaaat gcaggaagtc tttgcggaat ggaataaggg
     1321 agaactcaac tcgtatctaa tagaaataac cgcagatata ttgggaaagg tagatgaaga
     1381 aacgggagaa cctttggtcg acttgatact ggataaggcg ggacaaaagg gcaccggcaa
     1441 atggacatcc caggaagccc tggacctggg gaccccggcc cctaccatag cggaagccgt
     1501 atttgccagg tacatgtctg caataaagga tgagcgcatt aaggcttccc aagaattaaa
     1561 gggccctagg ccagcgtttg aaggcgataa gggacaattc atagaggata ttaaaagggc
     1621 cctatatgca tctaaaatct gttcgtacgc gcagggcttt gccctgatgg ccgaggcatc
     1681 gaaagcatac aactggaact tgaagctggg caatatagcc ctcctttgga gagccggctg
     1741 cataatcagg gcacagtttt tggacaagat taaagaggca tatgaaacaa atcccaagct
     1801 tcaaaacctg ctcctggcac cttatttcca gcagagcatc gaagactgcc aggaagcctg
     1861 gcggaaagta gtgagcacgg cagcattaaa gggagtcccc ataccttcct ttagctcggc
     1921 cctggcctac tatgattctt accgtacgca tacactgccc gccaacttaa tacaggcgca
     1981 gcgggattat tttggcgctc atacttatga gagagtagac agggaaggga ttttccatac
     2041 aatttgggtg tagtagaacg atgaaccttt acaaccctct cggtattaga tattttaacc
     2101 tccgtgtggt aaaatataat catcatgacc gggagggttt tatttgttta aaaggaaaac
     2161 ccaagggtca tgtcgaatac attataggtc acaaaatcgt actgggagtg tgaaaaatgg
     2221 ctaagctggg aagactcttt ttgataatag tggccgtggc agtggtatct ttctttgcca
     2281 tgagtgctt
//
LOCUS       DAONUO010000305         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_32454_length_2247_cov_3.740876, whole genome shotgun sequence.
ACCESSION   DAONUO010000305 DAONUO010000000
VERSION     DAONUO010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32454_length_2247_cov_3.740876"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..1954
                     /locus_tag="PK768_09505"
     CDS             356..1954
                     /locus_tag="PK768_09505"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA61247.1"
                     /translation="MILSQIINELSLPKEAILVGKAVLDFELNQVCLAMSLEDLTPID
                     LKHYLMVSPIDLFDADPALEHFESLAKDGKVASLLLTGPDEKIKASEIYKLCKQYNIS
                     LCCLGEDISPVLAINKIMGFYKTHMRNMEEIIQNHYLELIHLSTLDLGIPRIIQKSAE
                     ILGNPLVVTDESYDLVGYSSSGEVDDPIWKAIVESGYCPIDIVKILRYEGFEKQLERE
                     ASPLFLTQGEFSKYIRRLVTEIRIGGQLKGYMALLEYEKPITPMDQEILKFVSAVIAL
                     EFAKSDAIAKARGYLGQELLADLVKGNISSEASAWNRVKSLDWSLGEYFQVLLIANRE
                     NRSIGGEYYDSVQSIALRYIPASKISFSSKFIVILATSNSKDMLTKALPVLEKFCQQH
                     YLCIGIGRAYKTLMEIPQSFNEAQKALLLADSIERPGIVHYYDDIAVYDLLAKVKNNY
                     RVHPGLYRLIEYDKNNGTDYVKTLSVYLRNYKNLSQTAEDLFLHRNTILYRLKKIEEI
                     LQASLDNHSLCLQLELGILLLDLK"
BASE COUNT          687 a          453 c          492 g          615 t
ORIGIN      
        1 taagtcccgt tcctgcagcc gcccgagcag cgtaataggc tgccatccta tccgtgggct
       61 tgtgaaccaa gtgctcgcca aaggcggtac ccatgggagc tagaaacagc ctgtttttaa
      121 cctccattgt tccaatttta atgggcttga aaagatttgg gtaaactgcc attaaaaaat
      181 tcctcctttt cattcgtgat ttctttcaca tatttactta attcgatata atataactaa
      241 gtcttttgtg caagttatgg tttttaagcc ctttttcttg tgataattgt acaaaatgct
      301 tgccctgaca agatttttta gatacagtat aaaaaaactt agggggttac tcgccatgat
      361 attaagccaa ataataaatg agctttccct gccaaaagag gcaatccttg taggaaaagc
      421 ggttttggac tttgaactaa accaggtctg tcttgccatg agcctcgaag atttaacacc
      481 gatagaccta aaacattacc tcatggtttc ccccatagat ttatttgacg ctgacccggc
      541 attggagcat tttgaaagcc tggctaaaga cggcaaagtg gcttcgctgc tgttaacggg
      601 tcctgatgag aagattaagg caagcgagat ttataaactg tgcaagcagt acaatataag
      661 cctatgctgc ttaggtgaag acatatcccc ggtccttgcc ataaacaaaa tcatgggctt
      721 ttataaaacg cacatgcgga atatggagga aattatccaa aaccattatt tagagctgat
      781 tcacctgtcc acactggatt tgggcatccc gcgcataata caaaaatcgg ccgaaatcct
      841 gggcaatccc ctggtagtga cggatgaaag ctatgatctt gtgggctatt caagttcagg
      901 ggaagttgat gaccccatat ggaaggccat agtggaaagc ggatactgtc ccattgatat
      961 cgtaaagatt ttaagatatg aaggttttga aaagcaactg gaaagggagg cttcaccctt
     1021 atttctaacc cagggcgaat tctcaaagta tattcggcgc ttagttacgg aaatacgaat
     1081 cggaggacag ctgaaggggt acatggccct tttggagtat gaaaaaccca ttacgccaat
     1141 ggaccaggaa atactaaaat ttgtttccgc cgtcattgcc ctggagtttg ccaagtccga
     1201 tgccattgct aaagctagag ggtacttggg tcaagagctt ttagcagatt tagtcaaggg
     1261 caatatcagc agtgaggcca gcgcatggaa tcgggtgaaa tccctggatt ggtctttagg
     1321 agaatacttc caggtgctgc tgattgccaa cagggaaaac cgatctatcg gcggtgaata
     1381 ttacgacagc gtgcaatcca ttgcgctccg atacattcct gcttcaaaga taagcttttc
     1441 gtcaaaattc atagtaattc tcgccacgag caacagcaaa gacatgttga caaaggcact
     1501 tccggtcctt gagaaatttt gccagcagca ttacctatgt ataggcatag gacgggcata
     1561 taagaccttg atggaaattc cacagagctt caatgaggct cagaaggcct tgctgctggc
     1621 agatagcata gagagacccg gcattgtgca ttattacgat gacatagccg tatatgattt
     1681 gctggcaaag gtcaaaaaca actatcgggt ccatccgggg ctgtacaggc ttattgagta
     1741 tgacaaaaat aacgggacgg attatgtcaa gaccctttcc gtatatcttc gaaattacaa
     1801 aaacctcagc caaaccgctg aagatttatt cctgcatcgc aataccatcc tttaccgcct
     1861 taaaaaaatc gaagaaatat tgcaggcaag ccttgacaat cacagccttt gcttacaact
     1921 tgaacttggg atcctattgc tggatctaaa atgatctctc ttctattatg tgaattttaa
     1981 gtgagagtta agtttcaagc aagtgcctgg tgaaatgatt gaagtaatga aacagtattg
     2041 atataatatt ttcacaagat ttaaattcaa agtagggtgg tgtttgtttt gagtgctctg
     2101 aaagaacaag cccataaaat tatcgatctt attccggaag acaagatgag caaggtgcta
     2161 atcatactga aagaaattaa agatattatt gatgacgaaa tagacgattt tgatttacaa
     2221 cttgcaaaag aagcccagaa ggcttta
//
LOCUS       DAONUO010000306         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_32554_length_2241_cov_3.996798, whole genome shotgun sequence.
ACCESSION   DAONUO010000306 DAONUO010000000
VERSION     DAONUO010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32554_length_2241_cov_3.996798"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK768_09510"
     CDS             complement(<1..198)
                     /locus_tag="PK768_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HQA61248.1"
                     /translation="MNYFSRILKDYFKRNIIKYIFLSLILISGIIAGSITVNFLSDIQ
                     SVNLQRYISGFLANVNDVSVDY"
     gene            complement(267..1436)
                     /locus_tag="PK768_09515"
     CDS             complement(267..1436)
                     /locus_tag="PK768_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HQA61249.1"
                     /translation="MEYFADLHVHIGQAKNQPIKITASRRLTLDNIYDYCINKKGLDI
                     VGIIDCASPFVLEELREQFNEEKLVPLSGGGARYKDKLTVIFGAEIETEELRGVAHSI
                     AFFPFLEQIKDFSSVISEYITNINLSSQKARLSAKDLFKIVRNLDGVLIPAHVFTPFK
                     SFYGSCYDRLADAFEDDLDKIISVELGLSADTDFADTISELREKTFLTNSDAHSLEKI
                     AREYNKLHLQFPDFEHLVLALKGHNGNYIAANYGLDPKLGKYHRTFCPACNYVAKDIS
                     PITKCPFCNNEDVTLGVFDRIALIADHKTPIHPSGRPLYQYQVPLEFIPGIGKKTIEK
                     LILHFGTEMNVIHNVEKTELTEVVGERVAENIVLAREGKLNIEVGGGGIYGRVLD"
     gene            complement(1436..1978)
                     /locus_tag="PK768_09520"
     CDS             complement(1436..1978)
                     /locus_tag="PK768_09520"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQA61250.1"
                     /translation="MEFYETTLSSKQMFSGNLLKLRVDEVLLPNGRKSTREIVEHPGA
                     VAVVAITENDEVLMVRQYRKAIERELLEIPAGKLEEGESREVCVERELMEETGYFPNE
                     LTYLTSFYTSPGFSNEILHLFIAKNLIKKSRDADFDEYLQAEKVPFEEAINKIRTGEI
                     IDSKTITGLLLAYDQIKGDL"
BASE COUNT          701 a          453 c          352 g          735 t
ORIGIN      
        1 ataatcaacg gaaacatcat ttacatttgc taaaaaacca cttatatacc tttgcaaatt
       61 tacagactgt atatcggata aaaagtttac cgtaatgctc ccagcaatga taccactaat
      121 caaaataaga cttaagaaaa tatatttaat tatatttctc ttgaaataat ctttcagtat
      181 tctggaaaag taattcatta acgcagtcca ccttccgtac atattccata gaaaatatta
      241 tgccggaggg gactggatta tacatttcaa tctaaaaccc ttccgtaaat acctcctcct
      301 cctacctcta tgttcagctt tccttctctt gcaagtacga tattttcagc tactcgttcc
      361 ccgacaactt cggtaagttc ggttttttca acattgtgga taacattcat ctcggttcca
      421 aaatgtaaaa ttaatttttc aattgttttt ttgcctatac cgggaataaa ttccagcggc
      481 acctgatact gatataatgg cctacccgat ggatgaatag gtgttttatg gtcagcgatg
      541 agagcaatcc tgtcaaatac tcctagtgtt acatcttcgt tgttacaaaa gggacatttt
      601 gtgataggtg atatatcctt ggcaacatag ttacatgctg gacaaaaggt cctgtggtat
      661 ttaccaagtt tagggtcaag gccataattt gccgcaatat aatttccatt atgcccctta
      721 agtgctaaaa ctagatgctc aaagtcggga aattgcaaat gcagtttatt gtattcgcgt
      781 gcaatttttt ctagggaatg ggcatctgaa ttagttaaaa atgttttttc tctaagttca
      841 cttattgtat cggcaaaatc cgtatcagcg ctgagcccca attcaactga aataatctta
      901 tccaaatcat cttcgaaagc atctgcaagt ctatcataac agcttccgta aaagctttta
      961 aaaggagtaa aaacatgtgc cggaatcagc actccatcta gatttcgaac gattttaaat
     1021 aaatctttgg cgctaagccg ggctttttgt gaactcaaat ttatgttagt tatatattct
     1081 gaaatgactg aagaaaaatc cttaatttgt tcaagaaaag gaaagaaggc tatgctgtga
     1141 gcaactcccc ttagctcttc agtctctatc tctgctccaa aaattacggt aagcttatct
     1201 ttatatcttg cacctccacc ggataatggt actagcttct cttcattaaa ctgttctcgt
     1261 agttcttcaa gaacaaaggg tgaagcacaa tcaattattc caacaatatc taaacctttc
     1321 ttgttaatgc aatagtcata aatattatca agagttaacc ttcttgaagc ggtaatctta
     1381 ataggctgat tttttgcttg gcctatgtgc acgtgtaaat cagcaaaata ttccattata
     1441 aatcaccctt aatttgatca tatgccaata gcaatcctgt tattgtttta ctatcgataa
     1501 tttcaccggt tcggattttg tttatagctt cctcaaaagg cactttttca gcctgtaagt
     1561 attcatcaaa atcggcatct ctactctttt tgatgagatt cttagcaatg aacaggtgca
     1621 gtatctcatt ggaaaaacca ggagaggtat aaaaacttgt taagtacgtt aattcattgg
     1681 gaaaataacc cgtttcctcc attaattcac gttctacaca tacctccctc gattcacctt
     1741 cctcaagttt gcctgcaggt atttccagca actccctttc tatggctttt cggtactgtc
     1801 ttaccatcag tacttcatca ttctcggtaa tggcaactac agcaacggcc cccggatgct
     1861 ccacaatttc tcttgtggat ttcctcccgt ttggcaataa cacttcatcc acacgtagct
     1921 ttaataaatt cccagaaaac atctgcttag aacttagggt cgtttcgtaa aattccatta
     1981 tatgtttcca ctcccaaata acatattttt tgcccatata catataatat tttaagacaa
     2041 gtttaaggag aggtaaaggt ggaatatatc attcacaaag atgggcttta tatcgtcggt
     2101 aagccttcgg aaataaacca ttttttaaac aataaaacga aaatttatag aacattaaaa
     2161 gagtttcttg actttgaaaa tgaatatgaa aggacatggg caaataaaac ttttattctt
     2221 taaaaaaatc aagtcgcaaa g
//
LOCUS       DAONUO010000307         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_33343_length_2201_cov_2.472507, whole genome shotgun sequence.
ACCESSION   DAONUO010000307 DAONUO010000000
VERSION     DAONUO010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33343_length_2201_cov_2.472507"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2143)
                     /locus_tag="PK768_09525"
     CDS             complement(<1..2143)
                     /locus_tag="PK768_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQA61251.1"
                     /translation="MILVLLVAALISAFLGEIADAIAIAAIVVLNGIMGFVQEYRTEK
                     SLQALQQLAAPVAKVLRDGEVIAVPARDIVPGDIVLLGSGDRVPADGSLIVAENLRLD
                     ESLLTGESVPVKKTTSIDEESQSRIYKQNYVYMGTLVVNGRGKMVVEKIGMDTEMGKI
                     AGMIEDIGGEQTPLQKRLDLLGKQLVIICLAICGAVALLGVFRGEKIYDMFLFGVSLA
                     VAAIPEGLPAIVTIVLTLGVQRMIRKNVVIRRLPAVETLGCATVICSDKTGTLTENKM
                     SVREIYLGGETFLVTGTGYRIEGEFLYRDGKPLKSLPKELKRLLEIGVSCNNAGINIQ
                     RSGNVFEFLGGKKELVPYGDPTETAILMAGLKGKVNKHDVDADFTRLKEIPFDSDRKR
                     MSVLVKSRNGELFLFTKGAPDVLLPLCTKLQQRGEVRAITKTDVGNILNANEDMGRKA
                     LRVLGFAYKKLSINELEDSDLEKDLTFVGMMGLMDPPRPEAISAVQKCLTAGIRPVMI
                     TGDHKSTAWAVARELSMLTRDSRIVTGQELDNMSESELLKQIEHIAVFARVTPRHKLR
                     IVRSLKKKGHIVAMTGDGVNDAPAVKEADIGVAMGINGTDVTKEASSMILMDDNFASI
                     VAAVEEGRIIYDNIRKFLRYLLSCNVGEILTMIWTTMIGLPLPLLPVQILWVNLVTDG
                     LPALALGVDPPERDVMYRKPRGKSENVFSHGL"
BASE COUNT          541 a          622 c          418 g          620 t
ORIGIN      
        1 caagaccatg agaaaataca ttttcacttt ttcctcttgg tttgcgatac atcacatccc
       61 tttcaggggg atctaccccc agggccagtg caggaaggcc gtccgttaca aggtttaccc
      121 agagaatctg cacgggcaaa aggggcaagg gcaagcctat cattgtagtc catatcatgg
      181 ttagtatttc tcccacattg caggaaagga ggtaccgcaa aaacttgcgg atattgtcat
      241 atattatcct gccctcttcc actgctgcca cgatactggc aaaattgtca tccatcaaga
      301 tcatggaaga cgcttctttg gtcacatcgg tgccgtttat gcccatggcc acccctatat
      361 ccgcctcctt caccgccggc gcatcattaa ccccgtcccc ggtcatggcc acgatgtggc
      421 ctttcttctt caagcttctg acaattctca gcttgtggcg gggtgtgacc cgagcaaaga
      481 cggcaatatg ttcaatctgt tttaaaagtt cactctcgct catattatcc agttcctggc
      541 ccgtaacaat cctgctgtct cttgtaagca tggaaagttc cctggctaca gcccaggccg
      601 tggacttgtg gtcaccggta atcatcaccg gccttattcc ggcagtcaag cacttttgta
      661 cagccgaaat cgcctccggc cttggtgggt ccatgaggcc catcatacct acaaaggtaa
      721 ggtccttctc caagtccgaa tcttccaact catttatcga aagcttttta tatgcaaagc
      781 ctaacaccct taatgctttt cggcccatat cttcatttgc gtttaaaata ttccccacgt
      841 cagtttttgt aattgcccgt acctcacccc gctgttgtaa cttcgtgcaa agaggcagga
      901 gcacatccgg ggcaccttta gtaaatagga aaagttctcc gttcctggat ttcaccaaca
      961 ccgacatccg ctttctatcc gaatcaaagg gaatttcctt aagtctagtg aaatcagcat
     1021 caacatcatg cttatttacc ttgcccttta ggcctgccat caggatagca gtttccgtag
     1081 gatcgccgta aggtaccagt tcctttttgc cccctaaaaa ctcgaacaca ttaccgctcc
     1141 tctggatgtt tattcctgca ttattacatg atactccgat ttctaaaagc ctcttgagct
     1201 cctttggtaa actctttaat ggcttaccat ccctatataa aaattcgcct tcaattctgt
     1261 aacccgtccc cgtaactaaa aatgtctctc caccaagata aatttccctc acgctcattt
     1321 tattttccgt cagagtcccc gtcttgtcac tgcatattac cgttgcgcag ccgagggttt
     1381 ccactgcagg aagcctcctg attaccacat tttttcgtat catgcgctga actcccagcg
     1441 tcaagacgat tgttacaatg gcaggcaaac cttctgggat agctgctacc gccaagctca
     1501 ccccgaaaag aaacatatcg tagatttttt ctccccgaaa aacacccaac agggcaaccg
     1561 ctccgcatat ggccaggcag ataattacca gctgcttgcc taaaaggtcc agcctttttt
     1621 gaagaggagt ctgctctcct ccgatgtctt ctatcatccc tgcgattttg cccatctcgg
     1681 tgtccatacc tattttttca accaccattt tgcctcttcc attaaccact agggtgccca
     1741 tgtagacgta attttgtttg tatattctgc tttgagactc ttcgtcaata ctcgtggtct
     1801 ttttcaccgg aacggattcc ccggtcaaaa gggattcgtc aagtctcagg ttttctgcaa
     1861 caattaaact tccatctgcc ggcaccctat ccccgctacc aagaaggact atatctccgg
     1921 gaacgatgtc ccttgcaggt accgcaatca cctctccgtc gcgcaaaacc tttgccacag
     1981 gtgcagctag ctgctgcaat gcttgcaagg atttttccgt tctgtattcc tggacaaaac
     2041 ccattatgcc gtttaaaacc acaatggcag ctatggcaat agcatcggct atctccccga
     2101 gaaaagcgga aataagggca gccaccagca aaacaagaat cataaaatcc ttaaattggc
     2161 ttatgaaaat agagattgcc gatatttctt tgccctgctt t
//
LOCUS       DAONUO010000308         2176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_33812_length_2176_cov_4.415842, whole genome shotgun sequence.
ACCESSION   DAONUO010000308 DAONUO010000000
VERSION     DAONUO010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33812_length_2176_cov_4.415842"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /gene="recG"
                     /locus_tag="PK768_09530"
     CDS             <1..1948
                     /gene="recG"
                     /locus_tag="PK768_09530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQA61252.1"
                     /translation="INTIKDALLYFPRDYQDLSPLTFRQGREDQIGAFPCIVTGTPVS
                     KKYKRSLYITKVPVFDGQNKGYAIFFNQPYLQKSFYPRQRLLLIGKVKKAFGNFEILC
                     SEWIKLEKNKAIELESIRPIYSLTKGVSQNLIRNMVKSAIEFASELNETLPNSILKRY
                     NLLPLEESIKNIHFPKSFEMLEKARQRLIFEELLIFQLSTGVFRSFIRGQTRKNVYRI
                     LDLKPFLSKLPFVPTSGQVNVIKDIIKDLNSEYIMNRLVQGDVGSGKTLVACAALYLA
                     AKNGFQGVIMAPTEILAEQHHRTLQQFLDAHDINVGILKGGTSKKERDACLTRLKKGD
                     IDVLVGTHAVIQEDVEFKRLGMVITDEQHRFGVKQRENLVKKGQYPDVLVMSATPIPR
                     TVAMVLYSDLDLSIIDTLPAGRQKVETYVVQDHMRKRVYDFMASEVKKGHLAYVVCPA
                     VEENELDVVNVEEHREYLQKEYPFLKVEMLHGRMKQDEKDMILKKFLSKEIQVLVATT
                     VVEVGVDVPYATLMIVENAERFGLAQLHQLRGRVGRSSLKSFCILISGFAQGNARERL
                     NFLMNCHDGFEISQKDLELRGPGEFLGEKQHGFIEFKYASFTKDLETLKITQQLAQEI
                     LENEYLSLAEYKNLKDLIYSKINI"
BASE COUNT          743 a          356 c          482 g          595 t
ORIGIN      
        1 gattaataca attaaagatg ccctattata tttccccagg gactaccagg acctgtcccc
       61 attgaccttt aggcagggta gggaagacca gataggagct tttccttgca ttgttaccgg
      121 aactcctgta agcaaaaaat ataaacgaag tttatatata acaaaagtgc cggtttttga
      181 cggtcaaaac aaaggctatg caattttctt caatcaacct tatttacaaa agagcttcta
      241 tccccgtcag aggcttttgt taatagggaa agtaaaaaag gcctttggca actttgaaat
      301 cctatgctca gaatggataa aactggagaa aaataaagca attgagcttg agagcatacg
      361 accgatatat tctctgacta agggagtatc gcaaaacctt ataagaaata tggtaaagag
      421 tgccattgag tttgcatcag agctaaatga gacgctgcca aacagcattt taaagcgata
      481 caatctattg ccacttgaag agagtataaa aaacatccat ttcccaaaaa gctttgagat
      541 gctggaaaaa gctcgtcaaa ggctaatatt tgaggaactc ttgatatttc agcttagcac
      601 gggtgtattc agatctttta taaggggtca aacgcgaaaa aatgtgtatc gtattttgga
      661 tttaaagcct tttttatcta aacttccctt tgttcctact tccggccagg taaatgtcat
      721 aaaagatatt ataaaggacc ttaatagtga gtatattatg aataggctag tccagggtga
      781 tgtaggttcc gggaaaaccc ttgtcgcttg tgctgcactg tatctagcgg caaaaaatgg
      841 ttttcaagga gtcattatgg ctcctactga aattttggca gaacagcacc atcgaacact
      901 acaacaattt ttggatgctc acgatattaa tgtgggaatt cttaaagggg gcacgagtaa
      961 aaaggaaaga gacgcttgcc ttactaggtt aaaaaagggc gatatagatg ttttagtagg
     1021 aacacatgct gtcatacaag aagatgtgga atttaagcgc ttgggcatgg taataaccga
     1081 tgaacagcat cgatttggag taaagcagcg tgaaaatctt gtaaaaaagg gccagtatcc
     1141 ggatgtattg gtaatgagcg caacacctat accgagaacc gttgccatgg tgctgtacag
     1201 tgatttagat ttatccataa tcgatacttt gcctgcaggc aggcagaagg ttgaaacata
     1261 tgtggtacaa gatcacatgc gaaagagagt ttatgatttt atggcttcgg aagtaaaaaa
     1321 aggccacctt gcgtatgtgg tgtgtcctgc agtcgaagaa aacgagcttg acgttgtaaa
     1381 tgttgaagag cacagagaat acttgcagaa ggaatacccc tttttgaaag tagaaatgct
     1441 gcacggtagg atgaagcagg atgaaaaaga catgatctta aaaaagtttt tgtcaaaaga
     1501 gattcaagtg cttgttgcca caacggttgt agaagtaggc gtagacgtgc catatgctac
     1561 ccttatgata gttgaaaatg ccgaacgatt tggcctagcg cagcttcacc agctgagagg
     1621 cagagtagga agaagttctc ttaaatcatt ttgcattctg atatccggct ttgctcaggg
     1681 aaatgcaagg gaaagattaa actttttgat gaactgccat gacggctttg aaatatcaca
     1741 aaaagacctc gaattgagag ggccagggga atttttagga gaaaagcagc atggctttat
     1801 tgaatttaag tatgcaagtt ttactaagga cctagaaacg ctaaagataa cccagcaatt
     1861 ggcacaagag attttggaga atgaatactt atctttagct gaatataaaa acctaaagga
     1921 tttaatatat tcaaaaatta atatttgaaa ttaaaaaaca aaacgactac aagtgtttac
     1981 atactgtcaa ttgtagtcgt ttttttgtaa aaaacaacta aagcatgcat tggactgcaa
     2041 ggcagattgt tctagtatat agactttttc ttgggttata ttaagagtac agcaaataaa
     2101 ggaggtgaag caaaagtggc attaggtcaa aagagaaata agcttgtagt tcctgaagca
     2161 tataaagcta tggaac
//
LOCUS       DAONUO010000309         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_34105_length_2162_cov_3.669673, whole genome shotgun sequence.
ACCESSION   DAONUO010000309 DAONUO010000000
VERSION     DAONUO010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34105_length_2162_cov_3.669673"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PK768_09535"
     CDS             complement(<1..1173)
                     /locus_tag="PK768_09535"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HQA61253.1"
                     /translation="MRSIINKIKNMFKKSLVVEDIKQEKPAEKLLISPNLDKNVKYIK
                     AVFDRCSDLVVREFKIPAREPIDAALFYIDGMVDKNLTAANILRSLQFNPSFINDKNI
                     NKSNAFNIVKDQLLSIGEVTTAGDMDTLVQKVLSGQAVLLIDGTDKAIISSVRQLESR
                     SVDEPQTEPLVRGPRDGFVESIRTNITLVRRRIKSPRLKMELLKIGTISQTDVAVLYI
                     DGIANDKLVNEVKERLGRIEIDGVLESGYLEEFIEDCPYTIFPQVSSSERPDRMCACL
                     LEGRVGILVDNTPICLIVPATFPQFLQSPEDYYNRSPYATFTRTIRFITMHIALLLPS
                     AYIAVLTFHQEMLPTPLLISIAGQREGVPFPAFLEAVMMELVFEFLREAGARLPRPI"
     gene            complement(1311..>2162)
                     /locus_tag="PK768_09540"
     CDS             complement(1311..>2162)
                     /locus_tag="PK768_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQA61254.1"
                     /translation="ERTPLVWDDITYMVTLYNDDLEEIGKIEVKEHPGGRNYEITSGY
                     ITLTEPGLYLVSSTAWAAASDIYLVEILDSRGNPAKGADRVSYRIIGLDNFKVQKRYT
                     PGTFNDVSDSDWYSKAVASCYELGLIEGKGGSKFDPQGSISVAEALTIAARVNKIYYG
                     EGPEIEQTGSNWYDGAVYYAISRRIIYGDEFRVLTRPASRAELAYIFANALPDSEYKQ
                     INNISRLPDVNSETNYSHEIFKLYNAGILTGQDRNLTFNPDANISRAEVATIVTRLVR
                     PDNRVTF"
BASE COUNT          526 a          516 c          481 g          639 t
ORIGIN      
        1 tatgggcctt ggcagccgag cacctgcttc ccttaaaaac tcaaaaacca attccatcat
       61 gacagcttct agaaaggcag gaaagggcac tccctcccgc tgtcctgcga tggaaattaa
      121 aaggggtgtg ggcagcattt cctggtgaaa ggtaagcacc gcaatgtatg ccgacggaag
      181 taaaagggct atgtgcatgg ttataaacct tatagttcgg gtaaacgtag catatggaga
      241 cctgttgtag tagtcctccg gcgactggag gaactgtgga aatgtggcag gtactatgag
      301 gcatatgggc gtgttgtcaa ccaatatgcc gacacggcct tccagaaggc atgcgcacat
      361 gcggtcgggg cgttccgaac tactgacctg gggaaaaatt gtataggggc aatcctcgat
      421 gaattcttcc aggtatccgc tttcaagcac tccgtctatt tctatgcgtc ccagtctctc
      481 ctttacttca ttcaccagtt tgtcattggc gataccatcg atgtaaagta ctgctacatc
      541 ggtttgagaa atggtaccga tttttaaaag ctccattttg agccgggggc ttttgatgcg
      601 gcggcgaaca agggttatat tagtgcgaat gctttcaaca aagccatccc ttggcccacg
      661 gacaaggggc tccgtttgtg gttcgtcaac acttcggctt tccaattgcc tgacacttga
      721 aattatggcc ttgtccgtgc catcaattaa gaggacggcc tggcctgaca agaccttttg
      781 gactagagta tccatatccc ctgctgttgt cacttcgccg atgctgagca gttggtcttt
      841 tactatatta aatgcattac ttttgtttat atttttatca ttgataaagg aagggttaaa
      901 ctgcagcgag cgaagtatat ttgcggcggt taggttcttg tcaaccatgc catcaatata
      961 gaacaaagcg gcatcaatgg gttctctggc aggtatttta aactccctca ccaccaggtc
     1021 gctacagcgg tcaaacacag cctttatgta tttaacgttt ttgtcgagat tcggcgatat
     1081 caaaagcttt tccgcaggct tttcctgttt tatatcttca acgactaagc tctttttgaa
     1141 catatttttt attttattta taatgcttcg catcacttca cctaattttg ccttgttttt
     1201 tatgttattt tacccattaa aggcgaaaaa aacccgtaca taataaaaaa gagccactgc
     1261 gaagcttaag ctacgcagca gcccttttca atcgaattcg attccagaaa tcagaatgtt
     1321 accctgttat caggccttac cagtctcgtt actatggttg ctacttctgc cctgctgata
     1381 tttgcatcgg gattgaaggt cagattccgg tcctggccgg tgagtatgcc ggcattgtaa
     1441 agcttgaaaa tttcatgact atagttcgtc tcggagttta catcgggaag ccttgaaatg
     1501 ttatttattt gtttatactc actgtcgggt aaggcatttg cgaagatata ggccagttct
     1561 gccctacttg caggccgcgt gagcacccta aactcatcgc cgtagatgat ccgcctgctt
     1621 atggcgtaat agacggctcc gtcgtaccag ttggagccgg tctgttctat ttccgggcct
     1681 tcaccgtagt atatcttgtt tacccgggct gctatagtca aagcctcagc aacagatatg
     1741 ctgccctggg ggtcaaattt gctacctccc ttgccctcaa tgaggccaag ctcataacag
     1801 cttgcaaccg cctttgaata ccagtcacta tcgctgacat cgttaaacgt gcccggtgta
     1861 tatctttttt ggactttaaa attgtcaagg ccgatgattc tataagaaac cctgtcagct
     1921 ccttttgccg ggttgcccct gctgtctaag atctctacaa gatatatatc ggatgcagcg
     1981 gcccaggcgg tacttgaaac tagatacagg cccggttcgg ttaaagttat gtagcccgaa
     2041 gttatctcgt aattcctgcc acccgggtgc tcttttacct ctatcttgcc tatttcctcc
     2101 aagtcgtcat tgtacaaagt aaccatatat gttatatcat cccatacaag cggcgtccgc
     2161 tc
//
LOCUS       DAONUO010000310         2156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_34224_length_2156_cov_3.957163, whole genome shotgun sequence.
ACCESSION   DAONUO010000310 DAONUO010000000
VERSION     DAONUO010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34224_length_2156_cov_3.957163"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..907
                     /locus_tag="PK768_09545"
     CDS             56..907
                     /locus_tag="PK768_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQA61255.1"
                     /translation="MRKTTAFILIILLFIPAIALAQSVDYRFQIGGDIIIDSHETIKG
                     DVVTVLGDITVDGKVSGDVVAILGNVRVNGEVTGDVTSVGGTIIRGETSKIYGKSTEI
                     GVSGIKGLVNGIKRPGIHLDPGIFARNIRYSFFKVVRFLGLLAVGILVIILFPNAVKN
                     ASNLVEEEIGKKLLIGLAIFLLIPVAVILLFITLIGIPLIPIFLILLYAAGFFGYLCI
                     SIYIGRKLNERIIKKPEVLLEFVLGALILWLVQLVPFIGSLTSLAALIISLGIAADTR
                     FGSKAGE"
     gene            999..1820
                     /gene="cdaA"
                     /locus_tag="PK768_09550"
     CDS             999..1820
                     /gene="cdaA"
                     /locus_tag="PK768_09550"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HQA61256.1"
                     /translation="MSLQLFELFKGFRLMDLLDIAIVAYVSYKAIQLIQGTRAVQLLK
                     GLVVLLISTKLSEWLGLYTINWILRNTMTVGVIALLVVFQPELRRALEQLGRGKFFSS
                     SLLGLAEEELNKVIDHIAEAAEELSKGQIGALIVLEKETGLNEYIETGVKIGGSVSTE
                     LLINIFMPNTPLHDGAVIVRNDMIVAAGCYLPLSENPNLSKELGTRHRAALGITEETD
                     AVAIIVSEETGVVSVAKEGKLSRYLDVKTLKNMLRDIYEIKDKKPSLWHWRKNHA"
     gene            1813..>2156
                     /locus_tag="PK768_09555"
     CDS             1813..>2156
                     /locus_tag="PK768_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HQA61257.1"
                     /translation="MRNFFATDTALKIISIVAAIIMWLYVMNEQNPQVTYVIRDVPVR
                     LQNLDENKFALKDDSAEYKVNVKVKGRRSLVADIKPGDIYAYVNLRGRIEGDNLIRVD
                     ITTPTNIELIDVS"
BASE COUNT          636 a          385 c          522 g          613 t
ORIGIN      
        1 tgttcttcgc gcgtataata tatatatata taaataataa tgagagggat taacaatgag
       61 aaaaactacc gccttcattt taattatatt gctgtttata cctgcaatag cacttgccca
      121 aagtgtcgat tacaggtttc agatcggggg agatataata atagactccc atgaaacaat
      181 aaagggcgat gttgtcactg tcttgggcga tattaccgtt gacggcaagg tgtcgggcga
      241 tgtggtggcg atattgggca atgtaagggt caatggagag gtaacgggcg atgttacctc
      301 cgtgggagga actataatcc gaggggaaac ctctaagatc tacggcaagt caactgaaat
      361 aggagtttcc ggaataaaag gcttggtaaa tggcataaaa agacccggga tacacctgga
      421 ccccggaatt tttgccagga atatcaggta cagttttttt aaagtcgtcc gcttcttggg
      481 gcttttggcc gtggggatcc tcgttataat tctcttcccg aatgctgtca aaaatgcgtc
      541 gaatttggtt gaagaggaaa taggcaaaaa actgctaata ggtctagcta tctttttgtt
      601 gatacctgtt gccgtgatac tgttgtttat caccttgatt gggattcccc tcatacctat
      661 ttttttgatt ctactgtatg cggcaggttt ttttggatac ctctgcataa gcatatatat
      721 cggcagaaaa ctaaatgaac gaatcataaa aaaaccggaa gtcttactgg aatttgtgct
      781 aggcgccctt attctttggc tcgtacaatt ggtgcctttc attggaagct taacttctct
      841 ggcggcctta attatttccc tgggcattgc agcggatact cgttttggaa gcaaggcagg
      901 tgaatagaat cttgaaaaaa atcgccgtga aaatatacta atttaagatt aaatgctata
      961 atattttata tagcagaacg gagtgctggg gtggaaaaat gtcattacag ctttttgaac
     1021 tatttaaagg ctttcgtttg atggatttgc ttgacatagc aatagtcgca tacgtttcat
     1081 ataaggccat tcaattaatt caagggaccc gagcggtgca gctgttaaag ggcctggtgg
     1141 ttttgttgat ttctactaag ctaagtgaat ggttgggcct ttatacgata aattggattt
     1201 tgcgaaacac catgacggta ggagttattg cactgctggt agtttttcaa cctgaactta
     1261 ggcgggccct tgagcaattg gggagaggaa agtttttttc atcctccctt ttaggattgg
     1321 cagaagaaga gctgaacaaa gtaatcgacc atattgcaga agcggctgaa gaattgtcta
     1381 aaggtcaaat cggggctctc atagttttgg aaaaagaaac gggcctaaat gaatacattg
     1441 aaaccggggt taaaatcggg ggcagtgttt caactgaact cttgataaat atctttatgc
     1501 ccaatactcc actgcatgat ggtgcggtga tcgttagaaa tgacatgata gtagctgctg
     1561 gatgctatct tcccctttcg gaaaacccga atttaagtaa agaattggga accaggcacc
     1621 gggcggcctt gggcattaca gaagaaacgg atgccgtggc gattattgtt tcggaggaaa
     1681 ccggcgtagt atctgtagcc aaggaaggca agctcagccg ctatcttgac gtgaagaccc
     1741 ttaagaatat gttaagagac atatatgaaa taaaagataa aaaaccatcc ctttggcact
     1801 ggaggaagaa ccatgcgtaa tttttttgct acggatacgg ctctgaaaat catatccatt
     1861 gtggcagcta taattatgtg gctgtatgtc atgaatgagc agaaccccca ggtaacctat
     1921 gtaatcaggg atgtgccggt aagactgcaa aatttagatg agaacaaatt tgcccttaaa
     1981 gatgattctg ctgaatacaa ggtgaatgtc aaggttaagg ggcggcgcag cctggtggca
     2041 gacataaaac caggcgatat atacgcttat gtaaatttac gagggcgcat tgaaggggac
     2101 aatcttattc gggtagatat caccacccca accaatatag aattaattga cgtttc
//
LOCUS       DAONUO010000311         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_34697_length_2134_cov_2.851852, whole genome shotgun sequence.
ACCESSION   DAONUO010000311 DAONUO010000000
VERSION     DAONUO010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34697_length_2134_cov_2.851852"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PK768_09560"
     CDS             complement(<1..347)
                     /locus_tag="PK768_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HQA61258.1"
                     /translation="MVEPFVPFMPMEPKTCREAFDDRDTGFQVKWDGVRILAHIKDGQ
                     VQLYNRKKRLKTRQYPEIAQALKSLFNQDIILDGEMVALKEGKPNFPEIIRRDFATDT
                     GTIKHLAKLIPVT"
     gene            501..1391
                     /gene="ligD"
                     /locus_tag="PK768_09565"
     CDS             501..1391
                     /gene="ligD"
                     /locus_tag="PK768_09565"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HQA61259.1"
                     /translation="MPQILLEDEKLKLTNLSKVLWPDDNITKADFIKYYAQIAHVILP
                     HLKDRPMVFTRYPDGIYGKAFYQKNVPHYAPEWLKTVKIESDEGNITEYVVINDIKSL
                     IWAANQASLELHPWLSRISSLDNPDYVVFDLDPMENTDFQDVRKVALALRDLLEMEGL
                     VSFPKTSGSTGIQVYLPIACKYTYQQAREFAKLFCLAIEKTFPDITTTERSVSKREGK
                     LYLDYLQNVKGKTIISPYSPRPKKGAPVSCPISWEELEQGVTIDIFTIKNMPERISQK
                     GDIFEGVLSIKQNIDRWLNI"
     gene            1408..>2134
                     /locus_tag="PK768_09570"
     CDS             1408..>2134
                     /locus_tag="PK768_09570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA61260.1"
                     /translation="MAKVLVIGAGPAGLFAAAEAAKNGFDVTLYEKGKIGENIRCAEG
                     FFDTLKVLGKPQFGILYKVEQLIVKAVSTYKFSTDKLNLWMLDRASWQRKLAEEAVSS
                     GVKISENSQVRPEDLGKLKNGYDFIIDASGAPSVTSRAYGFSDHYKKNSGKTVQYTME
                     GDFSHIANCLKVGLLPDFWGYYWIFPKTKNIANVGVGNFYSARKDRLWNRLDRVIKEE
                     GISGYKVLKKTGGICPTAVPDALV"
BASE COUNT          651 a          442 c          461 g          580 t
ORIGIN      
        1 tatgtcaccg gaatgagctt tgcaagatgc tttatagtgc cggtatctgt agcaaaatcc
       61 cgccttatta tctcagggaa attaggctta ccctctttta acgcaaccat ttcgccgtcc
      121 aatataatat cttgattaaa aagagatttt aaggcctggg ctatttctgg gtactgtcga
      181 gtcttcaggc gtttttttcg gttataaagc tggacctgcc catcttttat gtgagccaat
      241 attcttacgc cgtcccattt tacctggaaa ccggtatctc tatcatcaaa ggcttcccga
      301 catgtttttg gctccatagg cataaaagga acaaaaggct ctaccataca atcaccctta
      361 aagtattttt tccacaaaaa gcgcatcaat tcatttcctg tttactgcag aatgttttga
      421 aaggaaggcg gatcgtctgt ttccatatta agccttttac atataaccaa attgttatat
      481 aatgattcgt aggtgataat ttgccccaga tattactgga agatgaaaag ctgaagctca
      541 caaatctgtc taaagtgtta tggcccgacg acaacataac aaaagccgat ttcattaaat
      601 attatgccca aatagcccat gtgatcctgc cccatttaaa ggacaggccc atggttttta
      661 ccaggtaccc ggacggcatt tacggcaagg ccttttacca aaaaaatgtg ccccattacg
      721 ctccagagtg gcttaagact gttaaaattg aatctgatga aggcaatatt accgagtacg
      781 tggtaataaa cgatattaaa agtttaatct gggctgccaa tcaagcatcc ctagaacttc
      841 acccctggct ttccaggata tcttccctgg acaatccgga ttatgttgtc tttgacctag
      901 accccatgga aaataccgat tttcaggatg tccggaaagt ggccctcgct ttaagggact
      961 tgcttgaaat ggaaggcctt gtgagtttcc cgaagacttc cggctccacc ggcatacaag
     1021 tttacctgcc catcgcttgc aagtatactt accagcaggc tagggaattc gcaaagctat
     1081 tttgccttgc tattgagaaa acctttcctg atataacaac taccgaaaga agtgtaagta
     1141 agagagaggg aaaactgtac cttgactacc ttcaaaatgt taaaggcaag actattattt
     1201 ccccgtacag tcccagaccc aaaaaggggg ctcccgtttc atgtcccata agctgggaag
     1261 agcttgagca aggcgtaact atagacattt ttacgattaa gaacatgccc gaacgcataa
     1321 gtcaaaaagg ggatatattt gagggtgttc tgtccattaa gcaaaacata gatagatggt
     1381 tgaacattta attattgagg tgattgtatg gcgaaagtcc tggtcatagg tgcaggtcca
     1441 gcaggccttt ttgcagcagc cgaggctgca aaaaatggct ttgatgtgac cctttatgaa
     1501 aagggtaaaa tcggtgaaaa tatcaggtgt gccgagggat ttttcgatac cctgaaagtc
     1561 cttggaaaac cccaatttgg aatactttat aaagttgaac agctcatagt aaaggcagtt
     1621 tctacataca aatttagcac cgacaaactg aatttgtgga tgctcgacag agccagctgg
     1681 caaagaaaac tggcagagga ggctgtgagt tcaggagtaa agataagtga gaattcacag
     1741 gtcaggccag aagatttagg caaactaaaa aatggctatg actttataat tgatgccagc
     1801 ggagcccctt cagttacatc gagggcatat ggcttttctg accattacaa aaaaaactcc
     1861 ggcaagacgg tccagtacac catggaaggc gatttttcac acatagctaa ctgtctgaaa
     1921 gttggcttat tgccagactt ctggggctac tactggattt tcccaaagac gaaaaatatc
     1981 gccaatgtag gtgttggaaa tttttattcg gcaagaaaag atagactttg gaataggctt
     2041 gacagggtaa taaaagaaga aggtataagt ggatataaag tgctcaagaa aaccggcggc
     2101 atatgtccaa ctgcggtacc cgatgcatta gtct
//
LOCUS       DAONUO010000312         2114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_35171_length_2114_cov_3.802817, whole genome shotgun sequence.
ACCESSION   DAONUO010000312 DAONUO010000000
VERSION     DAONUO010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35171_length_2114_cov_3.802817"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PK768_09575"
     CDS             <1..371
                     /locus_tag="PK768_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQA61261.1"
                     /translation="ERIIPVADITLDCLKLYISGARKELLSGKESKILFVNRQGAAIS
                     RQGVWKIIRKYSKKAGISKKITPHTLRHSFATHLLEHGADLRAVQEMLGHADISTTQI
                     YTHLTRSKIKEIYDNTHPRA"
     gene            428..1591
                     /locus_tag="PK768_09580"
     CDS             428..1591
                     /locus_tag="PK768_09580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQA61262.1"
                     /translation="MTGYKKKLVTILLISLLMIFIIKPAVAQTLVDISSSSATLMDAG
                     TGTILFEKNPHEKMEPASITKIMTLIIAFEAIESGKVNLSDIVRVSERAWKTGGSQVF
                     LGPGEEQSLENLLKCIVVASANDASTAVAEYIGGSVEGFVKLMNDKAKELGMTNSNFT
                     NPHGLSDPEHYTTAYDIALMSRELVKYPMFFKWSTIWMDYLEHTDKKRDATMLANTNK
                     LLGKYEGVDGLKTGYHSKAGHCFAGTAKRGDFRLISVVLNAQSSNQRFEDTVKLLDYG
                     FGHYDSIKVVEKGMVQKTIPVEKGHINEVNVIVTEDVSILIEKGREEHISTKIDVPEK
                     LFAPLNKNQAVGTLTVEQNGKAVKQIDLVISEDVMKAGFLELLKRVFGKWLSF"
     gene            1746..2084
                     /gene="spoIIAA"
                     /locus_tag="PK768_09585"
     CDS             1746..2084
                     /gene="spoIIAA"
                     /locus_tag="PK768_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HQA61263.1"
                     /translation="MDVNIEILNRDIIKVNLKGEIDHHATLVIKEDVDNYIEKHKLIK
                     ILFNFKDVTFMDSSGVGMIIGRYRKLQKIGGRIGVVNITPKIKRIFQMSGLFTLVSCF
                     NNEREAIDKL"
BASE COUNT          755 a          326 c          464 g          569 t
ORIGIN      
        1 aagaacgaat aattccagtg gcagatatta cacttgattg tttgaaacta tacatttcgg
       61 gggcaagaaa agagttgcta agtggcaaag agtctaaaat cttatttgtc aatagacagg
      121 gggcagctat aagtcgccag ggtgtttgga agatcatacg gaaatattct aaaaaagccg
      181 gtatttctaa aaaaataaca ccccatactc tcagacattc ttttgcaacc catcttctgg
      241 aacatggggc agatttaagg gcagtacagg aaatgttagg ccacgcagac atatcaacta
      301 cgcagatata tacccatctg acccggagca aaataaagga aatatatgat aatacccatc
      361 ctagagccta gcagtgcata acattcattg catatggaaa actatcataa gaagggagga
      421 ataatcaatg accggttata aaaaaaagct ggttacaata ttactgattt ctcttcttat
      481 gatttttata atcaaaccag ctgtagccca aacgctggta gatataagtt catcttctgc
      541 tactttgatg gatgcaggta caggaacgat tctctttgaa aaaaatcccc atgaaaaaat
      601 ggaaccagcc agtattacaa agatcatgac acttatcata gcttttgaag ctattgaatc
      661 gggaaaggtc aatctttctg atatcgtgag agtaagcgaa agggcatgga aaactggagg
      721 cagccaggtg tttctaggtc cgggggagga acaaagcctt gaaaacctcc tgaagtgtat
      781 tgtagtagct tcagcaaatg atgcatcaac ggcagtagca gagtatatag gcggcagtgt
      841 tgaagggttt gtcaagctta tgaatgacaa ggcaaaagaa ttggggatga caaacagtaa
      901 ctttacaaat ccccacggat tgtcggatcc agagcactac accacggcat acgacatcgc
      961 actgatgtcc agagagttag tgaaatatcc catgtttttc aaatggtcaa caatttggat
     1021 ggattatctc gaacacactg ataaaaagag ggatgccacg atgttggcaa acactaataa
     1081 gctattgggc aaatacgaag gtgttgatgg cttaaaaacc ggttatcata gtaaagccgg
     1141 ccattgtttt gccggcaccg ccaaaagagg agactttaga ttgatatcag tagttttaaa
     1201 tgcacaaagc agtaatcagc gatttgagga tacagtaaag cttcttgatt acggattcgg
     1261 ccattatgat tcgatcaaag tagtagaaaa aggcatggta caaaaaacaa taccggttga
     1321 aaaaggccat ataaatgaag taaatgtcat agtgactgaa gatgtcagta ttttgattga
     1381 aaagggcagg gaagaacata tcagcaccaa aattgatgtt ccggaaaaat tgtttgcacc
     1441 tttaaacaaa aatcaggcag tggggaccct tacggttgaa caaaacggca aggccgtaaa
     1501 acaaatagat ttggttatat ctgaagatgt aatgaaagca ggttttttag agctattaaa
     1561 aagagtattt ggtaaatggc tgagttttta atgagtttca atataaaggg cgtcatggtg
     1621 gcataaaaga cttccttagc tgggaagtct tttttaggtt aagaagaata tgtaaaatat
     1681 ggaactaatt agcaggatat tactcctgtt catcgaatta ttttaataga caaggggggt
     1741 ggaacatgga tgtaaatatt gagattttaa atcgagatat aataaaagtt aatttaaaag
     1801 gagaaataga tcatcacgca acacttgtga ttaaagaaga tgtagataat tacatagaaa
     1861 agcataagct aataaaaatc ctgtttaatt ttaaagatgt tacttttatg gacagctccg
     1921 gcgtaggtat gattattggt cgttatagaa aacttcaaaa aattggaggg agaattggag
     1981 ttgtaaacat tactccaaaa ataaaaagga tttttcaaat gtccggtctt tttactcttg
     2041 tcagttgttt taacaatgaa agagaggcta tagacaaatt ataaggggtg atattgtgac
     2101 agctttaaat gaga
//
LOCUS       DAONUO010000313         2102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_35464_length_2102_cov_4.019052, whole genome shotgun sequence.
ACCESSION   DAONUO010000313 DAONUO010000000
VERSION     DAONUO010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35464_length_2102_cov_4.019052"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1128)
                     /gene="aroB"
                     /locus_tag="PK768_09590"
     CDS             complement(67..1128)
                     /gene="aroB"
                     /locus_tag="PK768_09590"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQA61264.1"
                     /translation="MQTLQVNLKNKDYPVYIKRGIIKELGKSLRRLSSAKRVAIITDK
                     NLSRIYGAHVLNVLNPAGFETDVIAIEPGEKSKTLATLADVFEGLVKSRITRRDIIIA
                     LGGGVVGDLGGFAASSFLRGMPYVQVPTSLIAQVDSSIGGKVAVDLPQGKNLVGSFYH
                     PEAVFIDPDFLDTLDAREFSNGMAEVIKYGCIKDRQLFEELTGFDKNELLNNIETIIF
                     KCLSIKKKFVEEDETDQGARMMLNFGHTLGHAIEKYYHYAKYSHGEAVAVGMYRVTKN
                     SEDMGLTEPGTSQRLKEVLSKYGLPYEMPAGNAAEIVEVIAMDKKNKGDSIDIVLLNS
                     IGDGFIKNINRDEIPSFFL"
     gene            complement(1061..1981)
                     /locus_tag="PK768_09595"
     CDS             complement(1061..1981)
                     /locus_tag="PK768_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQA61265.1"
                     /translation="MDCSDFNITVVGLGLIGGSLAMAISKKIKTKQLWGVDIDRDALD
                     LAMASGIINKGFTDPKEPLQESDLVFVCIYPKAAVKFIRENMRHFKTGAIVTDVTGLK
                     GLIFREISPILRDDIDFIGGHPMAGNEFQGIQFASEKIFKGADYIMTPSAKSKRANVS
                     LLKDLLFKIGFANVVEMGPEQHDDMVAFTSHLPHIMALSLVLNPMIENTSICTGGSFR
                     DVTRVARINGDLWTELLLENASYVVKHLDIFLSNLQEFKKAILGNDSVSLRELFSKAH
                     KLKEELDLNADSPGKFKKQGLSGVYQARHN"
BASE COUNT          532 a          488 c          421 g          661 t
ORIGIN      
        1 ggttatctgg ccctttaatc cagatggagt aatttttacg cctttcataa aaacacctcg
       61 taatttttat aggaaaaaac ttggaatttc atctctattg atatttttta taaagccgtc
      121 acctattgaa ttcaacaata caatatcaat gctgtcgcct ttattttttt tatccatggc
      181 aatgacttca acaatctcgg ctgcatttcc tgcaggcatc tcatagggga ggccgtactt
      241 tgatagtact tctttaagcc tttgactggt tccgggctcg gttaaaccca tgtcttcact
      301 gtttttggtt acccgataca tgcctactgc taccgcttca ccatgggagt atttggcata
      361 atgataatat ttttctatgg catggcccag ggtgtggccg aagtttagca tcattcttgc
      421 tccttggtcg gtttcgtcct cttcaacaaa ttttttcttg atacttaaac acttgaagat
      481 aatagtttct atattgttaa ggagctcgtt tttgtcaaac cccgtaagct cttcaaatag
      541 ctgccgatcc ttgatacaac cgtacttaat cacttcagcc atgccattac tgaattctct
      601 cgcgtcaagg gtatccaaaa agtccgggtc aatgaaaact gcctcgggat ggtaaaaact
      661 gcccactaaa tttttgcctt ggggcaggtc aactgctacc ttgccgccta tactgctgtc
      721 aacctgggca atcagggaag tgggaacctg tacgtaaggc atacccctca aaaaggatga
      781 cgcagcaaat cctcccaaat cacctaccac cccgccgcct aaagctatga tgatgtccct
      841 gcgggtaatt cttgacttaa ccaatccttc gaaaacatcg gcaagggtcg ccagggtttt
      901 acttttttcg cctggttcga tggcaatcac atcagtttca aatcccgccg gatttaggac
      961 atttaagaca tgagctccat aaatgcgact taggttttta tcggtaatta ttgctactct
     1021 ttttgcagaa gataatcttc tcaaagactt gcccaattcc ttaattatgc ctcgcttgat
     1081 atacaccgga taatccttgt tttttaaatt tacctggaga gtctgcattt aaatcaagtt
     1141 cctccttcaa tttgtgggcc ttggagaaaa gctcccttaa agacactgaa tcgttgccga
     1201 gaatggcttt tttgaattct tgcaggtttg ataggaatat gtcaagatgt ttgactacat
     1261 agctggcgtt ttcaagcagc agctccgtcc ataaatcccc gtttatcctg gctacccttg
     1321 tgacatcccg aaagctgccg cctgtgcaga tcgatgtatt ttctatcatg ggatttagca
     1381 ccagggataa agccatgatg tggggcaggt gactggtgaa ggccaccata tcatcgtgct
     1441 gttcagggcc catctcaact acattggcaa aaccgatttt aaaaagcagg tcttttaata
     1501 atgagacatt agcccttttg ctttttgccg aaggagtcat tatatagtcg gcacccttga
     1561 agattttttc cgaagcaaat tgaattcctt gaaattcgtt tccggccatg gggtggccgc
     1621 cgataaaatc aatgtcatcc cttaaaattg gagatatctc tctgaaaatt aatcctttaa
     1681 gcccggtgac atcggtgaca atggcgccgg ttttgaagtg tctcatgttt tccctgataa
     1741 atttcactgc cgctttcgga tagatgcaga cgaagaccaa atcggattct tgcagcggct
     1801 ctttagggtc agtgaagcct ttatttatga tcccggacgc cattgccaag tccagcgcat
     1861 ctctgtcaat atcaactccc caaagctgtt tggtttttat ttttttgcta atcgccattg
     1921 ccagggagcc gcctatgagt ccaaggccca ctactgttat attaaaatcc gagcaatcca
     1981 tttaaaagcc tcctaaagct tgcgattgtg tattcgagct atttccctga ctctgtccat
     2041 taagcgtttg aagttttcag gtgtaacgga ttgattgccg tcacataggg cattcttagg
     2101 gt
//
LOCUS       DAONUO010000314         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_35489_length_2101_cov_3.459433, whole genome shotgun sequence.
ACCESSION   DAONUO010000314 DAONUO010000000
VERSION     DAONUO010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35489_length_2101_cov_3.459433"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /gene="proA"
                     /locus_tag="PK768_09600"
     CDS             complement(<1..389)
                     /gene="proA"
                     /locus_tag="PK768_09600"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295756.1"
                     /note="Catalyzes the phosphorylation of L-glutamate during
                     the proline biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HQA61266.1"
                     /translation="MVLNIAKQAKEASYVLSNASTAVKDKALAMMAKALEEMKGEIIN
                     ANQRDLENAREKGMKDAMLERLMLNDKRIDGMIKGLLDLIELPDPVGSGSIIKRPNGL
                     TLLKQRVPLGVVGIIYESRPNVTSDAAG"
     gene            complement(409..1218)
                     /gene="proB"
                     /locus_tag="PK768_09605"
     CDS             complement(409..1218)
                     /gene="proB"
                     /locus_tag="PK768_09605"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQA61267.1"
                     /translation="MKSNLYQTIVVKVGTTTLVHESGKLNFAGMEKLVRVISDIKNRG
                     KNVVLVSSGAVGTGAGRLGINGKGDIKIKQALAAVGQGILMQFYEKLFLEYGIIVAQV
                     LLTRDDIEKGVRRQNALNTFTTLFEFDVVPIVNENDTVAVEEIVFGDNDTLAAVVAKL
                     IRADLLVLLSDVDGLYTMPPHEPGAEKIPYVKEITPEIEALAEDSYSDFGTGGMLSKI
                     KAAKIATEAGIAMAIIDGEKPSDIYGIIDGTGCAGTFFEPKKHSFTDKNAM"
     gene            complement(1299..>2101)
                     /gene="amrA"
                     /locus_tag="PK768_09610"
     CDS             complement(1299..>2101)
                     /gene="amrA"
                     /locus_tag="PK768_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQA61268.1"
                     /translation="RIFDNKLVEILKTFDIKALKALEPSLIERAGECGLRSIWTMAGA
                     LDGYRVKSEVLSYEGPFGVGYCIARFKPDGRGDSILQELYQQRRDEIDERRAKEDPYV
                     RLARYTLETYVREGRIPELPDDLPEEMLSSKAGVFVSIKKHGQLRGCIGTYLPTRKNI
                     AEEIQRNAIAAGYEDPRFYPVGSDELEDLEYSVDVLTKPQPVDSKDMLDPKKYGVIVR
                     KGARSGLLLPDLEGVDTVEEQLRIVLAKAGIRPDEKYQIERFEVIRHV"
BASE COUNT          471 a          564 c          423 g          643 t
ORIGIN      
        1 cctgccgcgt ctgaggtcac attgggcctt gattcgtata ttatgccgac tacgcctagg
       61 ggtacccttt gctttagcaa tgtcaggcca ttcgggcgct tgattatgct tccgctgcct
      121 acggggtcgg gaagctcaat gagatccaaa agtcctttta tcatgccgtc gattcgcttg
      181 tcatttagca tgaggcgctc cagcatcgca tctttcatgc ccttttccct ggcattttct
      241 aaatcccttt gatttgcatt gattatttca cccttcattt cctccagggc ttttgccatc
      301 atggccaggg ccttgtcctt aactgccgtg gaagcatttg atagcacata ggatgcttct
      361 ttggcctgtt ttgcaatatt taaaaccatg cccaatcaaa tcactccatt acattgcatt
      421 tttgtcggta aaagaatgct ttttgggttc gaaaaaggtt cctgcacagc cggtcccgtc
      481 aatgataccg taaatgtccg atggtttttc cccatctata atggccatgg caattcccgc
      541 ctcggttgcg attttggctg cctttatctt gctcagcatg cctcccgtgc caaagtcaga
      601 gtagctgtct tcggccaagg cctcgatttc aggtgtgatt tcctttacat aaggtatttt
      661 ttccgctccg ggttcgtggg gaggcatcgt ataaagaccg tccacgtccg aaagcagcac
      721 caagaggtct gctcttatta actttgccac caccgccgcc agtgtgtcgt tgtcaccgaa
      781 aactatctcc tctaccgcca ccgtatcgtt ttcatttact atgggaacca catcgaattc
      841 aaaaagggtt gtaaatgtat ttaaagcatt ttgccggcgt acgccttttt ctatatcgtc
      901 ccgggtaagt aaaacctggg ctacaatgat gccgtattcc aggaacagct tttcgtaaaa
      961 ctgcatcaag attccctggc ccaccgccgc caatgcctgt tttatcttaa tatcgccttt
     1021 gccatttatg ccaagcctac cggcgcccgt gcccacagcc cctgatgaaa ccaggaccac
     1081 atttttgccc ctgttcttta tatcagaaat caccctgacc agtttttcca ttcccgcaaa
     1141 attcagtttg ccgctctcgt gtacaagtgt tgtagttcct actttcacaa ctatggtttg
     1201 atatagattg gatttcatgt cgtcacccca agtttaccaa aagtaattca ttcattgtat
     1261 tgtataatta taacacttgt atgcaaaaaa tcaatgagtc aaacatgtct aattacttca
     1321 aatctttcaa tttggtattt ttcatcaggc cttatgccgg ctttagccag gacaattctc
     1381 agctgctcct ctaccgtatc aacgccttcc aaatccggaa gtaagagacc ggaccttgct
     1441 ccctttctga caattacgcc gtactttttg ggatcaagca tatcttttga atcgacgggc
     1501 tgcggctttg taagtacatc caccgaatac tcaaggtctt ccaactcatc agagcctacc
     1561 gggtagaacc tggggtcttc atagcctgct gctatggcat tcctctgtat ttcttcggcg
     1621 atgtttttcc gagtgggcag ataagtgcct atgcaacccc gaagctggcc gtgctttttt
     1681 atagatacga aaactccggc tttcgagctt agcatttcct caggcaggtc atcgggaagc
     1741 tctggtattc tcccttctct tacgtacgtc tcaagagtat agcgggcaag tctcacgtaa
     1801 ggatcttctt ttgccctacg ctcatcgatt tcatcccgac gctgctggta tagttcctgg
     1861 agtatgctat ctccccttcc atcgggttta aacctggcaa tgcagtaccc gacgccaaat
     1921 ggcccctcat aggacagtac ttcggacttt acccgatagc catccagggc tcccgccatg
     1981 gtccagattg acctaagacc gcattctccg gccctttcga tgagggatgg ttctaaggcc
     2041 ttcaaggctt taatatcaaa ggttttcagt atttccacca gtttattatc aaaaatcctt
     2101 c
//
LOCUS       DAONUO010000315         2057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_36463_length_2057_cov_3.809690, whole genome shotgun sequence.
ACCESSION   DAONUO010000315 DAONUO010000000
VERSION     DAONUO010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36463_length_2057_cov_3.809690"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /gene="feoB"
                     /locus_tag="PK768_09615"
     CDS             <1..1109
                     /gene="feoB"
                     /locus_tag="PK768_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQA61269.1"
                     /translation="FTFIFSEPLVEIVETGFSTLSELLLAALTGIGASEIFISFVVDG
                     IVSGLGSVLSIVPVLFLLFIAIAMLEDSGYMARIAYVMDKPMRMMGLSGKAFVPFLLG
                     FGCNVSAVMATRTIENKRDRLITILANPFISCSARLPVYVLFTSVFFPKRQALVLGSL
                     YLLGVFMAIISVKLFGKIVPEQDDSSFIIELPPYRLPTLKGVFLHMWENVRGFLNKVS
                     NIILAAVVIIWLLSSLPFGVEYASDESMIGRMSSLIAPILKPAGFGTWQAAIALIFGV
                     LAKEIVISTLGVVYKAGSFGLAAVLSEHFTPLSAYSFMVMTLLYAPCIATIGSIRSET
                     KSLKWTAVSVIYGLIIAWTMSVLIYQIGLMLGFS"
     gene            complement(1289..>2057)
                     /locus_tag="PK768_09620"
     CDS             complement(1289..>2057)
                     /locus_tag="PK768_09620"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HQA61270.1"
                     /translation="TNTYCCPGYGSAAKFVATSVREMALDADVYDKEAVTIVEVMGRD
                     SGFLTAASALARDEVVDAPHLIYLPEVPFEEDVFLSDVERVFRKKGKVFITTSEGIKD
                     KSGNYPYLRDGKCDAFGNMQMGGVSRYLEHLVRENITKRVKVIEFSIIQRSAAHIASL
                     VDNQVATMVGKVGVEKILNGTSDVMISIEKTDTGFEAGTVPLAKVANYTKSIPIEWID
                     QQNRWVTQDAIEYMLPLIQGQVDIPYENGLPKYIRFI"
BASE COUNT          525 a          452 c          468 g          612 t
ORIGIN      
        1 cttttacttt tatcttcagc gaacccctgg tggaaatagt ggaaacgggc ttttccaccc
       61 tgagtgaatt gctgttggct gctttgaccg gcataggtgc ttcagaaata tttatatcct
      121 ttgtggttga cggaatagtg agcggtctgg gctcggtctt gagcatagta cccgtgttat
      181 tcctgctttt catagccatc gcaatgctgg aagatagcgg atacatggcg agaatagcct
      241 atgttatgga taagccgatg agaatgatgg gccttagcgg gaaggctttt gtgccctttc
      301 tccttggatt cggctgcaat gtctcggccg tcatggccac caggacaatt gaaaacaagc
      361 gggacaggtt aattaccatt ttggcaaacc catttatctc ctgcagcgcc cgccttccgg
      421 tatatgtact atttaccagc gtgtttttcc caaagaggca ggctcttgta ctgggatcat
      481 tatatttgct tggggtattc atggctataa taagtgtgaa gctttttggc aaaattgttc
      541 cggaacagga tgattcatcc tttataatag aactgcctcc ctacagattg cccactttaa
      601 aaggtgtatt ccttcacatg tgggaaaacg ttagaggttt tttaaacaaa gtcagcaaca
      661 tcattttggc agctgtggta atcatatggc tactttccag cttgcccttc ggtgtagagt
      721 atgccagcga tgagagcatg atagggagaa tgagttcact gattgcgcca attttaaaac
      781 cggcaggctt tgggacctgg caggcggcca tagccctgat atttggcgtg cttgcaaagg
      841 aaatagtcat aagcaccttg ggagtagtgt acaaggcggg cagctttggc cttgctgcgg
      901 tattgagcga gcatttcacc cccctgtctg cctattcctt tatggtgatg accctccttt
      961 acgctccctg tattgctaca atagggtcga taagaagtga aacgaaatct ttaaagtgga
     1021 ctgcagtttc tgtaatttac ggattaatta ttgcatggac gatgtcggtg ctcatatacc
     1081 aaatcggcct tatgctaggt tttagttaaa ggagaataat aatcatgcat gtaattgaaa
     1141 ttgtcataat atcggctgtc actcttgcgg caatttataa aatatataaa agtgcaaaag
     1201 gacaatataa ggattgtgac tgcagtatgt gcaataaaag ctgtaaaatg agaaaacact
     1261 tcccggactg agggaagtgt ttttgccatt aaataaagcg tatatattta ggtaggccat
     1321 tttcatacgg tatatctacc tgaccctgga taagagggag catgtattcg atggcatctt
     1381 gcgttaccca tctgttttgc tggtctatcc attctatggg gatggacttg gtgtaattgg
     1441 ccacctttgc aaggggaact gtgcctgctt caaaaccagt gtcggtcttt tcgatggaaa
     1501 tcatgacatc acttgtaccg ttcagaattt tctcgacacc cacttttcca accatggttg
     1561 ccacctgatt gtccactaga gatgctatat gggctgcaga cctttggata atggaaaact
     1621 ctattacttt gacccgtttt gtgatatttt ccctcacgag atgttcgaga tagcggctaa
     1681 cccctcccat ttgcatgttg ccgaaggcat cgcatttccc atcccggaga tagggataat
     1741 tgccgctttt atctttaatt ccctcagaag tggtgataaa taccttgccc ttcttgcgaa
     1801 aaactctttc cacatcactc aaaaaaacat cctcctcaaa gggcacttcg ggcaaatata
     1861 tcagatgggg tgcatcaact acctcgtccc tggccagtgc actggcggca gttaaaaaac
     1921 cgctgtctct tcccataact tcaacaatag taaccgcttc tttgtcatag acatctgcat
     1981 ccaatgccat ttcccgaacg cttgtagcca caaacttagc tgcactgccg tatcccgggc
     2041 aacagtaggt gtttgta
//
LOCUS       DAONUO010000316         2038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_36898_length_2038_cov_4.005547, whole genome shotgun sequence.
ACCESSION   DAONUO010000316 DAONUO010000000
VERSION     DAONUO010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36898_length_2038_cov_4.005547"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /gene="argJ"
                     /locus_tag="PK768_09625"
     CDS             complement(<1..871)
                     /gene="argJ"
                     /locus_tag="PK768_09625"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HQA61271.1"
                     /translation="MKYIDGGITSPKGFKAAGVASGIRKKGRKDVALVYSEQPAQAAA
                     VYTLNKFQAAPLQVTKEHLKDGIAQAIVVNSGVANACMGQQGLMSAKEMAQITADCLL
                     IKAEDVVVASTGVIGVPLPMELITKGIKDASKALSYDGGADAAEAIMTTDLVKKEVAC
                     QLEIGGKTVTIGAMAKGSGMIHPNMATMLGFITTDASIESDCLQHVLKKATDASFNMV
                     SVDGDTSTNDMVAILANGLAENPKITMESEYLKDFEAAVTEVCIRLAKMIAQDGEGAT
                     KLIEVEVVGAKSLE"
     gene            complement(898..1941)
                     /gene="argC"
                     /locus_tag="PK768_09630"
     CDS             complement(898..1941)
                     /gene="argC"
                     /locus_tag="PK768_09630"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQA61272.1"
                     /translation="MKIRVGIVGVTGYTGGELLRLLLNHPQAEVKKATSRSNAGNDVT
                     LVHPNLKGEISLIEDEFEIDSFVKDIDLVFLALPHGKSAPLAREIYDRDVKIIDLGAD
                     FRIKDAEAYKLWYELEHACPELLEESVYGLPEIHKDEIQKAMIVANPGCYPTSIILAL
                     APLLANKVISRENIIADSKSGVSGAGKTLTDTSHFANINENLHAYKVGNHRHIGEIEQ
                     ELSLLGQSQLLINFTPHLVPMNRGILSSVYAIPVVKLRQKELDELYHEFYKDCPFIRL
                     RDKDSLPQTKWVQGSNYCDIAPILDERTGRIIVFSAIDNLVKGASGQAIQNMNIMFGL
                     DETTGLKLAGMYP"
BASE COUNT          421 a          561 c          464 g          592 t
ORIGIN      
        1 cctccaggga tttcgcccct accacttcca cctcaatgag cttcgtcgcc ccttctccgt
       61 cctgggcgat catcttggcc aggcggatgc aaacttcggt aaccgccgct tcaaagtcct
      121 ttaagtattc gctttccatg gtaatcttgg ggttctcggc aaggccgttg gccaatatcg
      181 ccaccatgtc gttggtgctg gtgtcgccat ctaccgaaac catgttaaag ctggcgtcgg
      241 tggccttttt caacacatgc tgcaagcaat cactttctat gctggcatcc gtggtgataa
      301 agcccagcat ggtggccatg ttcgggtgaa tcatccccga gccctttgcc atggccccga
      361 tagtcacggt tttcccgcct atttccaact ggcaggcaac ttccttcttc accaggtccg
      421 ttgtcattat ggcttctgcc gcatcggccc ctccgtcata gctcaaggcc ttggaagcat
      481 cctttattcc tttagtgatg agctccatgg gcaggggcac gccgatgacc cccgttgaag
      541 ccaccaccac gtcttccgcc tttatcagca ggcagtccgc agttatttgg gccatctcct
      601 tggcgctcat taagccctgc tggcccatgc aggcattggc aacgccgctg tttaccacga
      661 tggcttgggc gatgccatcc tttaagtgct cttttgtaac ctgtaagggt gctgcctgga
      721 acttgttgag ggtatagacg gccgcagcct gggccggctg ttccgagtat accagggcca
      781 catcctttct gccctttttt ctgattccag aggctacacc cgccgcctta aagcccttgg
      841 gcgaagttat tccgccatct atatatttca tttgcatatt cctcccttaa atttagttta
      901 cggatacatg ccggccagct tcagacccgt tgtttcatcc aagccgaaca ttatgttcat
      961 gttctgtatg gcctggccgg aggccccttt taccagattg tcgattgcgg aaaacactat
     1021 gatccttccc gtcctctcat ctaggatggg agcaatgtcg cagtagttgg aaccctgcac
     1081 ccatttggtc tggggcaggc tgtccttgtc tctcagcctg ataaagggac agtccttata
     1141 gaattcatga tagagctcat ccagctcctt ttgccttagt ttcactaccg gtatcgcgta
     1201 tacggagctc agaatgcccc ggttcatggg gaccagatga ggcgtaaagt ttatgagcag
     1261 ttgggactgg cccaataggc ttagctcctg ttctatctcc ccgatatgcc ggtgattgcc
     1321 gactttgtat gcatgtaaat tctcgttgat attggcaaag tggcttgtgt ccgtcaaggt
     1381 cttcccagct ccgctgaccc ccgacttgct gtcggctatg atattttcac ggctgatgac
     1441 cttattcgcc aggagcgggg ccagggccag aattatgctt gtgggatagc agccgggatt
     1501 tgcaacgatc atcgcctttt gtatttcatc tttatgaatt tccggcaggc cgtaaacgct
     1561 ttcctccagc agctccggac aggcatgctc caactcgtac cagagtttgt aagcctcggc
     1621 atcctttatt cgaaaatccg cacccagatc tatgatttta acgtccctat cgtagatttc
     1681 cctggccagg ggtgcagact tgccgtgagg cagggccaaa aagaccaggt ctatatcttt
     1741 tacaaaggaa tctatttcaa actcatcctc gattaagctt atttcgccct tcaaattggg
     1801 atgtaccagt gtgacatcat tgccagcatt actcctggaa gtggcttttt tgacttcagc
     1861 ttgagggtgg ttgagcaaaa gcctgagcag ctctcctcct gtataaccgg tgacgccgac
     1921 aattccaact ctaattttca ttttccttcc cccaaaacta aaataatatt gtttataatt
     1981 atacacatat atgtataatt atgcaaccgt tattttaaat ttttattggg attttttc
//
LOCUS       DAONUO010000317         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_38151_length_1987_cov_2.565217, whole genome shotgun sequence.
ACCESSION   DAONUO010000317 DAONUO010000000
VERSION     DAONUO010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38151_length_1987_cov_2.565217"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PK768_09635"
     CDS             <1..423
                     /locus_tag="PK768_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HQA61273.1"
                     /translation="KDYIVSNATFPTYFLKQLDEAVDIQCMLDLKIFAEHIAPALGIK
                     KRYVGKEPYCPVTGVYNRIMKEILPGYEIEVIEVPRISLNGIVISASEVRKNLKAGNM
                     EVVKEMVPSTTYDFLVSKEAVPILQNIGYNFGGVGSGS"
     gene            413..712
                     /gene="citD"
                     /locus_tag="PK768_09640"
     CDS             413..712
                     /gene="citD"
                     /locus_tag="PK768_09640"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HQA61274.1"
                     /translation="MVLKNTGMAGTLESSDIMVVIEPKKHTGIEIILESPVERQFGDQ
                     IRKVILDTLDELGVKSAVVRAKDRGALDCVIKARTQAAVFRAAGSKDYNWGAIKR"
     gene            709..1617
                     /gene="citE"
                     /locus_tag="PK768_09645"
     CDS             709..1617
                     /gene="citE"
                     /locus_tag="PK768_09645"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HQA61275.1"
                     /translation="MRQLRRTMMYVPGNNPGLIRDAHIYGADSLMFDLEDSVSINEKD
                     AARFLVYNALLSIDYEGTETVVRVNGLDTPYGLDDLNAIVRAKPDIIRLPKTETAEDV
                     KKAERLLEEIERDCGMKVGTVKLMAAIESALGVVNAYQIATASPRLIGIAIGAEDFVT
                     DMKTERSKDGFELYAARTQVLLAARAAKIYALDTVYSDIRDDEGFEKEVRFIKQLGFD
                     GKSVIHPNQVEIVHRVFTPSPTEIQKSIRIIEAAKEAERRGLGVISLDGKMIDKPIIE
                     RAQRVLDLAEAAGLYKDGEKYEPKCS"
     gene            1598..>1987
                     /locus_tag="PK768_09650"
     CDS             1598..>1987
                     /locus_tag="PK768_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HQA61276.1"
                     /translation="MNLNAVNREFPPIIDGIGEIKAFAGTQFDSIKTDEKKCSKLVGN
                     LERAIKEAGLKDGMTISFHHHFRNGDYIVNMVMDTIAKLGIKDIRVAASSLTTIHEPL
                     IEHIKKGVITRIETSGLRGRLAEEISKG"
BASE COUNT          655 a          340 c          531 g          461 t
ORIGIN      
        1 aaggactata tagtctcaaa tgcaacgttt cctacatact ttcttaaaca actagacgag
       61 gcggtggata tacagtgcat gctggatcta aaaatattcg cagaacatat agcccctgcg
      121 ctgggaataa agaaaagata tgtaggaaag gagccttact gcccggtgac gggtgtctat
      181 aaccggatta tgaaagaaat acttccgggt tatgaaattg aagtgataga ggtaccgaga
      241 ataagcttaa atggaatcgt gataagcgca tcagaggtaa gaaaaaatct taaggctgga
      301 aacatggaag tggttaagga aatggttcca tcaacaacct atgatttttt ggtatccaag
      361 gaggccgttc caatattgca aaatataggt tataactttg gaggtgtagg cagtggttct
      421 taaaaataca ggtatggcag gcacactaga atccagcgat ataatggtag taatagaacc
      481 taagaaacat accggaatag aaatcattct tgaaagcccg gtcgaaaggc agtttggaga
      541 ccaaattaga aaggtaatac ttgataccct tgatgagctt ggtgtaaaaa gcgctgtggt
      601 aagagcgaag gatagagggg cattggactg tgtaataaag gcacggaccc aagcggcagt
      661 atttagagcg gcaggatcaa aggactataa ctggggggcg ataaaaaggt gagacagttg
      721 cggaggacaa tgatgtatgt accgggcaat aatcccggtt taattaggga tgcacatatt
      781 tatggagccg attctctcat gttcgacctg gaagattcgg tttccattaa tgaaaaggat
      841 gctgctcgtt ttcttgtata caatgcactt ttgagcatcg attatgaagg aactgaaaca
      901 gttgtcaggg taaatggcct tgatactcca tatgggttgg atgacttaaa tgcaatagtg
      961 cgggcaaagc ctgacataat caggctgcca aaaaccgaaa ctgccgagga tgtaaaaaag
     1021 gccgagcgtc tattggaaga gatagaaagg gattgtggca tgaaagtggg aaccgtcaag
     1081 cttatggctg ccatcgagag tgccttgggg gttgtaaatg cataccagat agcgacagcg
     1141 agtcccaggc ttataggcat tgccatcgga gccgaagatt ttgtcacaga tatgaagaca
     1201 gaaaggtcca aagacggctt tgagctatat gctgccagaa cacaggtgct gcttgctgct
     1261 agagctgcaa agatttacgc cttggatacg gtatactcgg atattaggga cgacgaaggg
     1321 tttgaaaagg aggtaagatt tataaagcag cttggctttg atggcaagtc agtgatacat
     1381 ccaaaccagg tggaaattgt acacagagtt tttactccaa gcccgacgga gattcaaaag
     1441 tccattagaa ttattgaagc ggctaaggag gccgagagga ggggtttggg ggtaatatcc
     1501 cttgacggaa agatgataga taaaccaata atagagcgtg cccagcgggt actggacctg
     1561 gcagaagccg caggattgta caaggacggt gaaaaatatg aacctaaatg cagttaacag
     1621 ggaatttcca cctataattg acggtatcgg agagatcaaa gcctttgccg gtacacaatt
     1681 cgacagcata aaaaccgacg aaaaaaaatg tagcaagttg gtggggaacc ttgaaagagc
     1741 gataaaggaa gccggactta aggacggtat gaccatttcc tttcatcatc atttcaggaa
     1801 tggcgattac atagtgaata tggtaatgga tacaattgca aaattaggta ttaaagatat
     1861 tcgcgtagcg gccagttcac tgacgaccat tcatgagcct ttgatagagc atatcaaaaa
     1921 gggcgtaata acacggatag aaaccagcgg actgaggggc cggcttgcgg aagaaatatc
     1981 aaaggga
//
LOCUS       DAONUO010000318         1974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_38473_length_1974_cov_3.680563, whole genome shotgun sequence.
ACCESSION   DAONUO010000318 DAONUO010000000
VERSION     DAONUO010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38473_length_1974_cov_3.680563"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PK768_09655"
     CDS             complement(<1..591)
                     /locus_tag="PK768_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA61277.1"
                     /translation="MNKIINWKKQFAIIYAGQAFSILGSAAVQFAIIWWLTIQTESAI
                     TLTIASVVAFLPNMLIGPFAGVWIDRYNRRSVMIAADGLVAVSSIILGAAFLLLQTPP
                     IWFIYIVLFIRGLGNTFHGPAMQAAIPMFVPTDMLTKAGGWGNMIQSVSNMMGPVLGA
                     ALMVFMPIYAIMVVDILGAAFAIVCLLLIIIPDIPQA"
     gene            complement(838..1620)
                     /locus_tag="PK768_09660"
     CDS             complement(838..1620)
                     /locus_tag="PK768_09660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQA61278.1"
                     /translation="MKNKTITEFELLIESHLDLERQGPGSAEMTLKALSFIMNLDSNS
                     KIADIGCGTGGQTMILAKNTGGHITGVDMVADFINIFNDASKKHNFSDRVQGIVGDAL
                     KLPFEKDGFDLIWSEGMIDSLGFEKTLSYWNGFLKTDGYIAVTSPSWLTTEKPAEIEK
                     FWIDAGSGLYSIESNIESMQKSGYSFVAAFTLPEECWIQNYFIPRQATEKELLIKYPE
                     DKTVEDYVHSMKYEVDLYEKHKQHYGYVFYIGKKMGEKLSRK"
     gene            complement(1610..1825)
                     /locus_tag="PK768_09665"
     CDS             complement(1610..1825)
                     /locus_tag="PK768_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61279.1"
                     /translation="MSVYSKEELFEAKRQIDSTIHKLTETLKTLESKENPDRYKSQVT
                     LVKRRIVAFEIAVNLLEKELKEIYDEK"
BASE COUNT          573 a          429 c          336 g          636 t
ORIGIN      
        1 ggcttgcgga atatccggaa ttataattaa caaaaggcaa actatcgcaa aagcagcccc
       61 cagtatatcc acaaccataa tggcataaat aggcataaat accatgagtg cagcacccag
      121 cacaggcccc atcatgttag atacagattg aatcatgtta ccccaaccgc ctgctttggt
      181 caacatgtcg gtaggcacaa acattggtat agccgcttgc atagccgggc catgaaaggt
      241 attgcccaat ccgcgtataa ataaaacaat gtagataaac caaataggcg gtgtctgtaa
      301 aagtaaaaaa gctgcgccaa gaatgatgct ggatacggct actaagccgt cagctgcaat
      361 cattaccgag cgacgattgt atctatcgat ccatacaccg gcaaatggtc cgattaacat
      421 attggggaga aaagcaacaa cggatgcaat cgtcagtgtg attgcggatt cagtctggat
      481 ggtaagccac cagataatgg caaactgcac tgcggcagaa cccaatattg aaaaagcttg
      541 tcctgcatat attatagcaa attgtttttt ccagtttatt attttgttca tttttaagaa
      601 atctcctttt ataaaatcag tttatgcaac attacaagct gcacatttac aaaaacataa
      661 ttttataagg aagatttgga atcttatttt ccttatagac tatgttttta aacacaacaa
      721 ataaactcct aagagttcgt gcttaaaaac atatattaca tgaatactat ccacataggt
      781 cattgcctca cttcctgttt ttaattttat tgcataaaag cgtactggta aggtttgcta
      841 cttcctgctc aacttctcac ccattttttt gccgatgtaa aaaacatacc cataatgctg
      901 cttgtgcttt tcatataaat caacctcata cttcatactg tggacatagt cctctacggt
      961 tttatcttcc ggatatttta tcaaaagctc tttttctgtt gcctgacgtg ggataaaata
     1021 attttgaatc caacattcct ctggtaatgt aaatgctgca acaaaactat accccgattt
     1081 ttgcattgat tcgatattgc tttctatcga atacaaacca ctacctgcat caatccagaa
     1141 cttctctatt tcggcaggct tttctgtggt aagccacgag ggactggtta cggctatata
     1201 tccatctgtt ttgagaaagc cattccagta gctaagtgtt ttttcaaacc ctaagctgtc
     1261 tatcattccc tcagaccaga taaggtcgaa cccatctttt tcaaaaggga gttttagggc
     1321 gtcgccgaca ataccttgca ctctgtcgga aaaattatgt ttcttggaag catcgttaaa
     1381 aatgtttata aaatcagcta ccatatctac gcctgtaata tgaccaccag tattctttgc
     1441 aagtatcatc gtttgaccgc ctgtaccgca gccgatatct gctatctttg aattgctgtc
     1501 aaggttcatt atgaaactta gggcttttag cgtcatttca gcacttcctg ggccttgcct
     1561 ttctaagtcg agatggcttt cgattaataa ttcgaattct gttattgttt tatttttcat
     1621 cgtatatctc ctttagctct ttttccagca aattcaccgc aatttcaaaa gcgacgattc
     1681 gtctttttac aagtgtaacc tgcgatttat atctatcagg attttccttt gactcaagtg
     1741 ttttaagtgt ttctgttagc ttgtgaatgg ttgaatcaat ctgccgtttg gcttcgaaca
     1801 gttcttcttt cgagtacacg ctcattttat ctcctttatt ttttcaaggt cggcactaca
     1861 caaagcctca aggttatcaa aaatcttttt agcaggccat tcccaccact ttaattccag
     1921 cagatagcca atgagttcat cctcaaacct tttcttaata attctgatag gatt
//
LOCUS       DAONUO010000319         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_38683_length_1966_cov_3.940869, whole genome shotgun sequence.
ACCESSION   DAONUO010000319 DAONUO010000000
VERSION     DAONUO010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38683_length_1966_cov_3.940869"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK768_09670"
     CDS             <1..213
                     /locus_tag="PK768_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61280.1"
                     /translation="LYAFIAWSIQQVYKRSENLAVELLNEVSVMFQDTFFSKVMVKEL
                     PDTLAVIPALYLLNQLNKKCQLLLTK"
     gene            227..619
                     /locus_tag="PK768_09675"
     CDS             227..619
                     /locus_tag="PK768_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61281.1"
                     /translation="MASNADRFLKAVEDAEVLTETIKKLQEEITSYQAAGQNLDSVRK
                     DLNKQLQTAYKISENMSGLIKTIAAFGPEIIANQKEHANQISSLNEKTTNEIITLRQN
                     LDKIRLLIVFSIVVSAVAAVISLITLFV"
     gene            751..1641
                     /locus_tag="PK768_09680"
     CDS             751..1641
                     /locus_tag="PK768_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQA61282.1"
                     /translation="MHPDELKEKILNIRIWQRGDERAPHKPLLLLYSLARVTRGEPRL
                     VSYADAVDDLRKLLKEFGPYRKVHYPSYPFVKLCNDGGFWEIEGNRPLNTTRDWSDRD
                     LIENNTSGGFTAEAYNILKKDNSLLKELASLILNQYFPETLHEDILAQVGLDLGSGAK
                     TVRSPDFRNRVLRAYEYQCAVCGFNVRLGDTLVAVEAAHIKWHQYGGPDDEENGIALC
                     SMHHKLFDKGVFTLDKSLVFWVAEEAHGTSGFDEWLMRFHGRPIRRPQRPDYYPNEDY
                     INWHVREVFRGPSRHGKGYE"
     gene            1634..1858
                     /locus_tag="PK768_09685"
     CDS             1634..1858
                     /locus_tag="PK768_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61283.1"
                     /translation="MNEYLLEQMLKENPELIEPGLTFVEHEDITLFRYPDSDWTNVEE
                     MVKELVPCICDYVERMNSLLNHYRPKKNKT"
BASE COUNT          603 a          366 c          453 g          544 t
ORIGIN      
        1 ctttatgcat ttattgcttg gtctattcaa caggtatata agcgcagtga aaatctggcg
       61 gtagagttgc tgaacgaggt ttctgtgatg ttccaggata catttttcag caaagttatg
      121 gtgaaagaac tcccggatac tttagcagta atcccagcgt tatatctatt aaaccagcta
      181 aacaaaaaat gccaactatt attaactaag taggaggagt gaagtaatgg ccagcaatgc
      241 agatcgtttt ttaaaagcag tggaggacgc cgaagttctt acagagacga ttaaaaaatt
      301 gcaagaagaa attacttctt atcaagcggc aggacaaaat ttggatagtg tcaggaagga
      361 tctcaacaaa cagttacaga ctgcttataa aattagcgag aatatgagcg gtttaatcaa
      421 aacgatagct gcttttggtc ccgagattat cgccaatcaa aaggaacatg caaatcaaat
      481 cagtagccta aatgaaaaaa caacaaatga aattataact ttacgacaaa atttggataa
      541 aattcgtctt cttatagtct tttcaattgt agtatctgct gtagcggctg ttatcagttt
      601 aataactttg tttgtttaaa aagatggatt attcgagttc tgaagcccga tttcatctac
      661 tttgggctct gaatgacctt gcctagggaa tcttgttatt gggaaagatt caaaatctac
      721 ttcaaagatt aaaggaagag agtctcccag atgcatccgg atgagcttaa agaaaaaata
      781 ttgaatatcc ggatctggca aagaggcgat gaacgggcgc cccacaagcc gctcctgctt
      841 ctttacagcc ttgctcgtgt gacaaggggg gagcccaggc ttgtttccta tgcagatgca
      901 gttgatgact taagaaagct tttaaaagaa ttcggaccat accgcaaggt gcactatccc
      961 agctatccct ttgtcaagct ctgtaacgac ggtggtttct gggagattga aggcaatcgg
     1021 cccttaaata ccacgcggga ctggtctgat cgcgatttga ttgaaaataa cacttccggt
     1081 ggatttacgg ctgaagcata taatatattg aagaaagaca acagcctctt aaaagaactt
     1141 gccagtctta tacttaatca atacttcccc gaaacactgc atgaggatat attggcccaa
     1201 gttggcctgg atctgggaag cggtgccaaa acagtccgca gcccggactt tcgaaatagg
     1261 gtcctccggg cctatgaata tcaatgtgct gtctgcggct ttaacgtcag gctgggagat
     1321 accttggtag ctgttgaagc ggcccatata aaatggcacc agtacggagg accggatgac
     1381 gaagaaaacg gaatcgccct ctgttccatg caccataagc tttttgataa aggtgtattt
     1441 actctcgaca aatcccttgt tttctgggta gcagaagaag cgcacggaac ttctggtttt
     1501 gatgaatggc tgatgcgctt tcacggcagg cctatacgca ggccgcaaag gccggattac
     1561 tatcccaatg aggactatat caattggcac gtccgagagg tatttcgtgg cccttcaagg
     1621 catggaaaag gatatgaatg aatatctatt ggagcaaatg ctcaaagaaa accctgaact
     1681 aattgaaccg gggcttactt ttgttgagca tgaggatatt actttattca ggtatcctga
     1741 tagtgactgg acaaatgttg aagaaatggt taaggagctg gtaccttgta tatgtgatta
     1801 tgttgagaga atgaattcct tgcttaatca ttatagacct aagaaaaaca aaacatgaat
     1861 tttttgtgtt ggcccgcaga ttggctagag atgcaattat ttgtgaatgg aggaataggg
     1921 tttggctatc gctacttttg acagtacgaa agttttatta gtagat
//
LOCUS       DAONUO010000320         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_39020_length_1953_cov_2.907271, whole genome shotgun sequence.
ACCESSION   DAONUO010000320 DAONUO010000000
VERSION     DAONUO010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39020_length_1953_cov_2.907271"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PK768_09690"
     CDS             complement(<1..624)
                     /locus_tag="PK768_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61284.1"
                     /translation="MTLKVTPEQEKEVKAFGFLRNKGTDNFNGRIITVNGKITAAQQR
                     CVAEAAEKFGNGDVVFTTRLTIEVPGIPYDKIEDFRAYIAREGLETGGTGSKVRPIVS
                     CKATTCQYGLLDGYAFSEEIHERFYKGYADVRLPHKFKIGVGGCPNNCIKPDLNDLGI
                     IGQMVPVFDEELCFGCKKCAVVDVCPMGAAKVVDGVLNIDEELCNNCG"
     gene            complement(650..1348)
                     /locus_tag="PK768_09695"
     CDS             complement(650..1348)
                     /locus_tag="PK768_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61285.1"
                     /translation="MAQNLNGDSKSYKGVLGTAVAKLPGLNIGKTGLTEDAAKAEGHD
                     VVTVVIVVDDKAHYYPGASNFIVKMIADKSNKKLLGLQVLGEGAVDKMIDIAAVAMAL
                     NATLDDIKDLDLAYAPPFSTAIHPFSHTVNILLNKINGDIQSITPREYLEGKAEGYKI
                     IDASISPNIPGVPFVDLSKVHGELSEFDKDEKLLLVCTKGKRAYMLQKRLKSFGYTNT
                     LVLEGGTSFNEVSL"
     gene            complement(1333..>1953)
                     /locus_tag="PK768_09700"
     CDS             complement(1333..>1953)
                     /locus_tag="PK768_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA61286.1"
                     /translation="TGADLIVPPVDGIDLKGVYFMRTPEDAITLRQAIEAGEIKRALV
                     VGGGFIGLEVAENLAGQGVRVSVIDMAEQILPGFGPEIASFVENHLAEQGIMTFTDTK
                     LEAILGDGKVEKVKTSKRPMKVDAVIFSTGVRPNTGFLSDTGIKLGKNGAIIVNEHME
                     TNIEDIYALGDCAVVSNTMTGKEAYSPMGSSANIQQIFKVESWPKI"
BASE COUNT          478 a          488 c          374 g          613 t
ORIGIN      
        1 gccacaattg ttgcacagtt cttcatcgat gtttaatacg ccatctacga cttttgccgc
       61 gcccatcggg catacatcaa cgacggcaca ttttttgcag ccaaagcaca gttcttcatc
      121 aaaaacaggc accatctggc cgataatccc gagatcattt aaatcaggtt taatgcagtt
      181 attgggacat cctcccacac ctattttaaa cttatggggc aacctaacat cggcatatcc
      241 cttataaaac ctctcgtgta tttcctccga aaaggcatat ccgtcgagca ggccgtattg
      301 gcaggtagtt gccttgcagg aaactatcgg ccggactttt gaaccggtgc ctcccgtttc
      361 aaggccttcc ctggcaatat atgccctaaa atcttctatc ttgtcgtagg gaattccggg
      421 aacctctata gtcagcctgg tggtaaatac cacatcgcca tttccgaatt tttctgcagc
      481 ctccgctacg catctttgtt gagccgctgt tatttttcca ttaaccgtga taattctccc
      541 gttaaaatta tcagtcccct tatttctaag aaagccaaaa gcttttacct ctttttcctg
      601 ttctggagta acctttaaag tcatttttgc accttcctat ttcataattc tataaagata
      661 cttcattgaa gctagtgcca ccttcaagaa ctaaggtatt tgtgtagcca aaacttttca
      721 gtcgcttttg cagcatgtag gcccttttgc ccttcgtgca gaccaatagc aatttttcat
      781 ctttatcaaa ttcggatagt tcaccatgaa ccttggatag gtcaacaaaa ggaacaccag
      841 gaatgtttgg gctaatcgat gcgtcaatta ttttatagcc ttcagccttg ccttccaagt
      901 attctctagg tgtaatggat tgtatgtcac cattgatttt attgagtaat atatttacag
      961 tgtgagaaaa ggggtgaatt gccgttgaaa agggtggcgc gtatgctaag tctaggtcct
     1021 ttatgtcatc taaagtagca tttaaggcca tggccactgc tgctatgtct atcatcttat
     1081 ctaccgcacc ttcaccaagc acctgcaatc ccaacagttt tttattgctc ttgtccgcta
     1141 tcatttttac tataaaattt gaagctccgg gataataatg ggccttgtca tcaactacga
     1201 ttacgactgt tactacatca tggccttcgg ctttagcagc atcttccgtt aggccggttt
     1261 ttccaatatt caatccgggg agcttggcga ctgcagttcc gagaacacct ttatagcttt
     1321 tgctatcgcc gtttagattt tgggccaaga ttctaccttg aatatttgct gaatatttgc
     1381 tgaagagccc attggtgaat aagcttcctt tcctgtcatc gtgttgctta caacagcaca
     1441 gtctccgagg gcatatatgt cttcaatatt agtttccata tgctcattga cgataattgc
     1501 tccgtttttg ccgagcttta tgcccgtatc agataggaag ccagtattgg gccttacgcc
     1561 tgttgaaaat atgacggcat ctactttcat gggccgcttg cttgttttca ctttttcaac
     1621 ctttccgtct ccgagaatcg cttctaattt tgtatccgta aaggtcataa ttccctgctc
     1681 tgccagatgg ttttcaacaa agcttgctat ctccggccca aagccgggaa gtatttgctc
     1741 tgccatatcg atgacggaaa ccctaactcc ctggccagcc aagttttcag ccacttccag
     1801 gcctataaag cctcctccga ccacaagggc tcgcttaatc tctccggcct caatggcctg
     1861 ccgaagagtt atggcatcct ccggagtcct cataaaataa acgcctttaa ggtcgatacc
     1921 atctacaggt ggtacaatga ggtctgcacc ggt
//
LOCUS       DAONUO010000321         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_39242_length_1944_cov_3.406035, whole genome shotgun sequence.
ACCESSION   DAONUO010000321 DAONUO010000000
VERSION     DAONUO010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39242_length_1944_cov_3.406035"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="PK768_09705"
     CDS             complement(<1..1573)
                     /locus_tag="PK768_09705"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQA61287.1"
                     /translation="MQYYILRNKNPITLISGILIAAAFISKLAIGRETVFHWSLIAAS
                     IIGAAPIAIQAYQAMRVKVVSIDVLVTIAVIGAFFIRNYEESAIVTFLFLFGAYLEQR
                     TLNKTRSAIKELTEMAPESALKQMENGEFEEVEVDDVDVGDILLVKTGAKVPVDGTVL
                     SGEGSVNEASITGESIPVSKEKGSKVFAGTILDNGTLQIVADRVGEDTTFGKIIELVE
                     EAQDSKSEVERFIDRFSKNYTPAVLVLSFVVWIFTRDVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARHGVLLKGSEVISDFSRVDTMVFDKTGTLTVGNPSVAEKEFYGDSIE
                     EALGYLASIERESDHPLAKAVLEEIGDTTFYPVENTEVVKGEGIVANVNGHRVVVGNV
                     ALMEKEKVRLDEKAKADMDRLEKNGNSLVLTAVDGELKILMGVRDRIRPGVKEDLQKL
                     KRLGVKNLIMLSGDNQGTVDVVSRELGLSEAHGNMLPEDKADYIKKLLEAGRIVAFVG
                     DGVNDSPSLALANIGI"
     gene            complement(1597..>1944)
                     /locus_tag="PK768_09710"
     CDS             complement(1597..>1944)
                     /locus_tag="PK768_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61288.1"
                     /translation="MILWKRESRNIKRKRGVIMTAFNEVKARHLEKLQQFVPIVARVH
                     GGSHPEFHQVHKLFAEIDAKIREAGSEKPNLDKEFKELRQVTGNYSVPEDVCETYEAV
                     YNMLAELDKAYHG"
BASE COUNT          455 a          541 c          371 g          577 t
ORIGIN      
        1 ctatgccgat atttgccagg gccagcgaag ggctgtcgtt gactccatcg ccaacaaatg
       61 ccacgattcg acccgcttct aaaagtttct taatgtagtc tgccttgtct tcgggcaaca
      121 tgttcccatg agcttcgctt aggcccaact cacggctcac cacatctacc gtgccctggt
      181 tgtcgcccga aagcataatc aggtttttta cacccaatct ctttaacttt tgcaggtctt
      241 ccttcacacc cggtcgtatc cggtcgcgga cgcccattaa tatttttaat tccccatcca
      301 cggcagttaa gaccagggag tttccgtttt tttcaaggcg atccatatca gctttggcct
      361 tctcatcaag ccttaccttt tccttctcca tcaatgccac attgccgact accaccctat
      421 gtccgttgac atttgctaca atcccttcgc cctttacaac ttccgtgttt tcaacggggt
      481 agaatgtggt gtcaccgatt tcctccaaaa ccgcctttgc caaggggtga tcggattccc
      541 gctcaatgct ggcgagatat cctaatgctt cttcgatact gtctccgtag aactcctttt
      601 ctgcaacgga tgggtttccg actgtcaggg taccggtctt gtcgaatacc atcgtatcca
      661 ctctgctgaa atcgctgatt acttcactgc cctttagcag cactccatgg cgcgcaccat
      721 tccctatgcc tgccacattg gataccggca ctcctataac caaggcgccc gggcagccca
      781 ggaccaggat ggtaatggcc agctccacat ctctcgtaaa tatccaaacg acaaaagaca
      841 ggacaagcac agccggcgta tagtttttcg aaaagcggtc tatgaagcgc tccacttccg
      901 attttgagtc ctgggcttct tccaccagtt cgatgatctt accgaaggtg gtatcttccc
      961 ccacccggtc agccacaatt tgaagggtcc cgttatctaa aattgtgcct gcaaatacct
     1021 ttgagccttt ttccttgctg acgggaatgg actctcccgt aatgctggct tcattgacac
     1081 tgccctcacc ggataaaacc gtgccatcga cgggaacttt cgcacccgtt ttaaccagca
     1141 gtatatctcc tacatctaca tcatccactt caacttcttc aaattcgcca ttttccatct
     1201 gttttaatgc gctttccggg gccatttccg tcaattcctt tatggccgaa cgggtcttgt
     1261 ttaatgtgcg ctgctccaag taagcgccga acagaaataa aaatgtgact atggccgatt
     1321 cttcataatt ccgaataaaa aaggcaccta taactgctat tgtaactaaa acatctatac
     1381 taacaacttt tacccgcatg gcctggtatg cctgaatggc tattggcgct gcaccaatga
     1441 tagaggcagc aatcaatgac cagtggaaga cagtttccct tccaatggcc aatttgctga
     1501 tgaaagcagc ggctattaaa ataccgctta ttagagttat tggattttta tttcttaaaa
     1561 tatagtactg cactgttttc tactcctaag atatttttat ccatgatatg ctttatcaag
     1621 ttcagccaac atattgtaca cggcttcgta ggtttcgcac acgtcctccg gcactgaata
     1681 gtttccagta acttggcgca gttctttgaa ctccttgtcc agattcggct tttcagaacc
     1741 tgcttctctg atctttgcat cgatttcagc aaacaacttg tgaacctgat ggaattccgg
     1801 atggctcccg ccgtggaccc tggccacgat aggcacaaat tgctgtaatt tttcaagatg
     1861 tctagctttt acctcgttaa aagctgtcat gataactcct cgctttcttt ttatattcct
     1921 tgattcccgc ttccataata tcat
//
LOCUS       DAONUO010000322         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_39660_length_1929_cov_2.989861, whole genome shotgun sequence.
ACCESSION   DAONUO010000322 DAONUO010000000
VERSION     DAONUO010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39660_length_1929_cov_2.989861"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      116..227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            356..1390
                     /locus_tag="PK768_09715"
     CDS             356..1390
                     /locus_tag="PK768_09715"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HQA61289.1"
                     /translation="MIYIKDLTKIYHSDTGDVKALEDVNLHIKQGEIYGVIGLSGAGK
                     STLLRCINMLETPTAGTIEIDGVEMTRLSPYALKEYRKKIGMIFQHFNLLSSRTVWEN
                     VAFPLEIAGINKQKIKDKVNNLLELVGLTDKANSYPSQLSGGQKQRVGIARALANDPK
                     VLLCDEATSALDPQTTLSILNLLKDINAKFGLTIVLITHEMNVIKQICHSVAVIEKSR
                     VVEQGPVLEVFSNPKTETAKNFLKDITLSELPDELKERIRHLEQTNLDGKIVKIGFFG
                     EITTQPIIATMVKKFGINANILFGNIDYIQDDPYGVLIVELNGQNGRANEALDYLKGL
                     GLKVEVIENV"
     gene            1383..>1929
                     /locus_tag="PK768_09720"
     CDS             1383..>1929
                     /locus_tag="PK768_09720"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HQA61290.1"
                     /translation="MSDIMRLLLVSTWESLYMVFSSVAIATFFGIPLGVLLFVTDKQQ
                     ILKNDLINQVLSWIVNIIRSIPFVILLILLLPFTKLIVGKAIGTTAAIVPLSIAAIPL
                     VGRLTETALREVPKGVIEAAQSMGASPLQIISKVLLPESLPSIASGITITTINLIGYS
                     AMAGVIGGGGLGDVAVRFGYNR"
BASE COUNT          580 a          403 c          432 g          514 t
ORIGIN      
        1 ttcccctata tccgcaaatt tattcaaatg cggaataata ttcttgcatc aaactttatt
       61 gacaaaagaa taaaagcatg ttattattaa gagcaataaa atgaatagcg gaaaactctt
      121 atcgagagca ggttgaggga ctggcccgat gacacccggc aaccggcagt gttcagcact
      181 gcagcggtgc taattccagc agaacagtta gttctgacag atgagagagt gattaaaacc
      241 tcttcttcat ggtgtcagaa ggggtttttt gttgtaaatg gcctgttttc tcggtcaata
      301 aaagtgtttt cattgctttg tagttaactt gttatatagg agaggttgtt aaacaatgat
      361 atacatcaag gaccttacaa aaatttatca ttccgacacg ggagatgtta aagctcttga
      421 agatgtaaac cttcacatta aacaggggga aatatacggc gttataggct tgagcggtgc
      481 cggcaaaagc accctccttc gctgcatcaa catgctggaa acacccaccg ccgggactat
      541 agaaattgat ggtgttgaaa tgacaagact gtctccttac gccctgaagg aatacaggaa
      601 gaaaatcggc atgatctttc agcactttaa tttgctttct tcaaggacgg tttgggaaaa
      661 tgtagcattt cctctggaaa tcgcaggtat caataaacaa aagataaaag acaaagtgaa
      721 taatcttttg gaactggtag gcttaaccga caaggccaac agctatccat cccaactttc
      781 gggaggtcaa aaacagcgtg tgggtatagc cagggctttg gccaacgacc ccaaggttct
      841 cctttgcgat gaggctacct ctgctttaga ccctcaaact accctttcta tcctcaatct
      901 gttaaaagac ataaacgcaa agtttggcct taccatcgtc ctaataactc acgaaatgaa
      961 tgtcatcaaa cagatttgtc attcggtagc cgtcatagag aagagcagag ttgttgaaca
     1021 gggcccggtg ctggaagtct tttcaaatcc aaaaacggaa acggccaaga atttcttaaa
     1081 ggatataacc ttgtcggaac tgcccgatga gctaaaggag cgaatcaggc atctggaaca
     1141 gactaacttg gatggcaaaa tcgtaaaaat cggctttttc ggtgaaataa ctacacagcc
     1201 cataattgcc acgatggtca agaaatttgg catcaatgcc aacatcctct ttggaaacat
     1261 cgattatata caggatgacc catacggtgt cctgatagtg gagctaaacg ggcaaaacgg
     1321 cagggcaaat gaagcattag attatcttaa aggtcttggg cttaaagtag aggtgattga
     1381 aaatgtctga cataatgaga ttactgctcg tttctacatg ggaatctcta tatatggtat
     1441 ttagttccgt ggccatagcc actttcttcg gcataccgct gggagttttg ctgtttgtaa
     1501 ccgataagca gcagattttg aaaaatgacc ttataaatca agttttgtcc tggatagtaa
     1561 acattatcag gtccattccc tttgtcatac tactgattct cctgctgccc tttactaaac
     1621 taatagtagg taaggccata ggaaccaccg cagccatcgt gcccctatca attgcagcaa
     1681 tacccctagt aggcaggctg acggagacgg ctttgaggga ggtacctaaa ggtgtcatag
     1741 aagctgccca gtcgatggga gcatctcccc ttcaaataat ttcgaaggtc ttgcttcctg
     1801 aaagtctacc ttccatagcg tcaggaatta ccataactac cataaattta attggctact
     1861 ccgccatggc gggtgtaatc ggagggggtg gtctcgggga tgtggccgtg agatttggat
     1921 acaaccggt
//
LOCUS       DAONUO010000323         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_39664_length_1929_cov_2.615795, whole genome shotgun sequence.
ACCESSION   DAONUO010000323 DAONUO010000000
VERSION     DAONUO010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39664_length_1929_cov_2.615795"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PK768_09725"
     CDS             <1..785
                     /locus_tag="PK768_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQA61291.1"
                     /translation="VWERSNRFVLKNSAAFIVIGRCMADRIRKKLCDLDVEEMRMKQH
                     IIHVWADDRNIQPLPEGTENIYRKRWNLEGKFVLGYSGNMGRFHDMETIAEAARELRE
                     YEDIRFVFVGEGYKKKWLQTYVETNRLTNCVIDTYVPRKDLSLLLNAFDIGLVSLLPG
                     QEGLSVPSKTFGLLAAGVPVIAIMNPNAEIAMVVKEESCGFVTPPGDSKALKSIILYC
                     YTNRATLKTMKTNARKTVDEKYSLNKAAQAYVSLISEINNLK"
     gene            814..1782
                     /locus_tag="PK768_09730"
     CDS             814..1782
                     /locus_tag="PK768_09730"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQA61292.1"
                     /translation="MKKALVCGAGGFIGAHLVKRLTKEGYWVRGVDLKYPPYANTHAH
                     DFIIGDLRDPKVCRYVVDQPFDEVYQLAADMGGAGFVFTGDNDADIMHNSALINLNMI
                     EACWKRNIKKIFYSSSACIYPEYNQKDPDNPKTAEDSAYPAQPDSEYGWEKLFSERLY
                     LAFHRNYGIDVKIARFHNIFGPEGSWNDGREKAPAALCRKVAMAEDGGEIEMWGDGNQ
                     TRSFLYIDECLEGIRRLMESDWTGPVNIGSEEMVTINQLAEMIMNVAAKKLRIKHIPG
                     PTGVRGRNSDNRLIYKKLGWKPTQPLRNGVEITYKWIEEQVKKHKS"
BASE COUNT          623 a          380 c          440 g          486 t
ORIGIN      
        1 aggtttggga aagaagcaac cggtttgtcc tcaaaaattc agctgccttc atcgtaattg
       61 gaaggtgtat ggctgataga attcgcaaaa aactctgcga tcttgatgta gaagagatgc
      121 gcatgaagca acatattatt catgtatggg cagacgacag aaacatccaa cccttacccg
      181 aagggactga aaatatctat cgaaaaagat ggaatcttga aggcaaattt gtcctcgggt
      241 attctggcaa tatgggacga tttcacgaca tggaaacgat tgctgaagct gcaagagagc
      301 tccgggaata cgaagacata cgttttgtct tcgttggcga gggctataag aagaaatggc
      361 tccagaccta tgttgaaacg aatagactta ctaactgtgt gattgacact tatgtcccga
      421 gaaaagattt atcgctgctt cttaacgcct tcgatattgg cctcgtaagc cttttgccgg
      481 gacaagaggg tctatctgtg ccttcaaaaa cctttggact ccttgcagcc ggagtccctg
      541 ttattgcaat tatgaaccct aatgctgaaa tagccatggt ggttaaggaa gaatcctgtg
      601 gcttcgtcac tcctccaggc gatagtaagg cccttaaaag cataattttg tattgctata
      661 caaatagagc taccttaaaa acaatgaaaa ctaatgccag aaagaccgtt gatgagaaat
      721 attctttaaa taaagcagct caagcttacg ttagtttgat atctgaaata aataatttaa
      781 aataaattat aaaatataaa ggagttgatc attttgaaaa aagccttagt ttgtggagca
      841 ggcggtttta taggcgccca cctcgtcaag cggttgacaa aagagggcta ttgggtccgc
      901 ggagtagatt taaaatatcc accatatgct aacactcatg cgcatgactt cataatcgga
      961 gatcttcgag atccgaaagt ctgcaggtat gtagttgacc agccattcga tgaagtttac
     1021 caacttgccg ctgacatggg aggggccggc tttgttttta caggagacaa tgatgcagac
     1081 ataatgcata attcagccct aatcaattta aacatgatcg aagcctgttg gaaaagaaat
     1141 atcaaaaaga ttttttattc ctcttcagct tgtatttatc ctgaatacaa tcagaaggat
     1201 ccagacaatc ctaaaaccgc cgaagattcg gcctaccctg cccagccaga tagtgaatat
     1261 ggctgggaaa aactcttcag cgaacgcctc taccttgctt tccacaggaa ctacggtata
     1321 gatgtaaaaa tagctcgttt tcacaacatt ttcggccccg aaggttcatg gaatgacggt
     1381 cgggaaaagg cgccagcagc actttgtagg aaagtagcaa tggcagaaga tggcggagaa
     1441 attgagatgt ggggtgatgg aaatcagacc cgctctttcc tttacattga tgaatgcctt
     1501 gaaggtatcc gacgtcttat ggaatccgat tggacaggtc ccgtaaacat cggttccgag
     1561 gaaatggtca cgataaacca gcttgcggaa atgatcatga atgtggccgc taaaaaactt
     1621 agaataaagc atatccctgg tcccactgga gtaaggggaa gaaattcaga caacaggtta
     1681 atatataaaa aacttgggtg gaaacccaca cagcccttaa gaaatggagt agaaatcacc
     1741 tacaagtgga tagaggaaca agttaaaaag cataaatctt agcaacgcca agtgttgtaa
     1801 atctggagat aggggtctac catctatcta cacgacatgg tagacccaag gatttatgta
     1861 acgagactgc tgaaaaatca cttaacagcg gaaccatcag cgaagaagcc tcgatacttg
     1921 ggttaagag
//
LOCUS       DAONUO010000324         1873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_41233_length_1873_cov_3.022002, whole genome shotgun sequence.
ACCESSION   DAONUO010000324 DAONUO010000000
VERSION     DAONUO010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41233_length_1873_cov_3.022002"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="dnaG"
                     /locus_tag="PK768_09735"
     CDS             complement(<1..1334)
                     /gene="dnaG"
                     /locus_tag="PK768_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQA61293.1"
                     /translation="MVKLGFYSDEIIKEVQDRADILEIVSEYTSLKKRGDNYIGLCPF
                     HSEKTPSFNVDVEKKLFYCFGCGVGGNVFTFIMNKENLSFPEAVRFLADKFNVRLPEK
                     INSKLSQEFRERREYYKINRFAAKFFNYCLLHTRDGSRALSYIKQRGLTEETVEKFFL
                     GYAPSSWDGLIKYARKMGIDSATLEKAGLAIARKDKQGHYDRFRNRLMFPIEDVTKNI
                     IGFGGRALDDSMPKYLNSPETPLFSKGDNLYALSKIEKDPALDVIIVEGYMDCITLHQ
                     HGFRQTVASLGTALTQKQAKLLKKYTGGVILSYDADAAGEAATERGMEILAQEGLRVK
                     ILSLPKGKDPDEIIREQGGQSFQKLLDSSLDLVSYKLQKAKLGIKIETADGKLKYIKK
                     AIDILCQINNEPELEIHIKNLAEQLGISVQALKKEVYRKKLANNGLQYKKSQT"
     gene            complement(1491..>1873)
                     /locus_tag="PK768_09740"
     CDS             complement(1491..>1873)
                     /locus_tag="PK768_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HQA61294.1"
                     /translation="KVLGKKHGQRIDTMIKDVIATSFGRPFVKMSDEIYDATRRLRDF
                     MFDNVYVGSTAKTQENKAKTMLRLLFEYYTGHPESMPPEFVKIAEEENVERAVVDYIA
                     GMTDGFALKKFKDIYVPSSWPLEI"
BASE COUNT          508 a          421 c          317 g          627 t
ORIGIN      
        1 gtttggcttt ttttatactg caatccattg tttgccaatt tctttctgta tacttctttc
       61 tttaaagcct gaactgatat gcccagctgt tcagctaggt tttttatgtg tatctcaagt
      121 tccggttcat tatttatctg gcatagaata tctatcgcct ttttaatata tttaagcttt
      181 ccatcagcag tctcaatctt tatgccgagt tttgcttttt gtagtttgta gctgaccaag
      241 tccaaggaac tatccaacag tttttggaag gattggccac cctgttccct tattatttca
      301 tccgggtctt ttcccttggg cagacttaat atttttaccc taagcccttc ctgggctaat
      361 atttccattc ccctctccgt agctgcttca ccagctgcat cagcatcgta agagagtatt
      421 acgccaccgg tgtacttttt taatagtttt gcctgttttt gcgtaagggc agttcccagc
      481 gaggctacgg tttgtctgaa accatgctgg tgcaatgtta tgcaatccat gtagccttct
      541 acgatgataa catcgagggc agggtccttt tcgatctttg aaagggcata caaattatcg
      601 cccttggaaa acaagggggt ttccggagaa ttgagatatt tgggcatcga atcatcaagg
      661 gctcgcccac caaaaccaat aatatttttg gtcacatctt caatgggaaa cattagtcta
      721 tttctaaatc tatcgtaatg gccttgcttg tccttgcgag caattgcaag gcccgctttc
      781 tctaaagttg cagaatctat gcccattttc ctggcatatt taattaggcc atcccaagat
      841 gatggtgcat atccgagaaa aaacttttca actgtttcct ctgttaaacc ccgttgctta
      901 atatatgaca gggctctaga accatcacga gtgtgaagta aacagtaatt aaaaaattta
      961 gccgcaaagc ggtttatttt ataatactcc cgtctttccc taaattcttg agatagcttg
     1021 ctattaatct tttcagggag ccgtacatta aacttgtcgg ccaaaaacct tacagcttct
     1081 ggaaagctaa ggttttcttt attcataata aaagtaaata catttccgcc tacgccacat
     1141 ccaaaacaat aaaacagttt tttttctaca tcaacgttaa aagacggcgt cttttccgaa
     1201 tgaaatgggc aaaggcctat ataattatcg ccgcgctttt taagagaagt gtattcggat
     1261 acaatttcca atatgtcagc tctgtcctgc acttctttaa tgatttcatc agaataaaag
     1321 cccaatttta ccaccgaacc ttaaaaaaaa tctccccata gccaaatgag gagctattgc
     1381 cactgttaat tttggtattc tactatcccc cctcaatatt acataacaat attctacgtt
     1441 aatctcgatt attccttctt tttgtcagat aaaaatatca ggtttatttt ttagatttcc
     1501 aagggccagg atgaaggcac atagatatct ttaaactttt tgagggcaaa accatcagtc
     1561 attcccgcaa tatagtctac tactgccctc tccacattct cctcttctgc tatttttaca
     1621 aattcgggag gcatggattc cggatgaccg gtatagtatt caaataaaag tcgcaacata
     1681 gtcttggctt tattctcctg tgtctttgca gtcgaaccca cgtaaacatt gtcaaacata
     1741 aaatcccgca atcttcgagt tgcatcataa atctcatcgc tcatctttac aaaaggtcgg
     1801 ccgaaactcg tagctataac atctttaatc atggtatcga tgcgctgtcc gtgtttcttt
     1861 cctaaaacct taa
//
LOCUS       DAONUO010000325         1859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_41640_length_1859_cov_2.453991, whole genome shotgun sequence.
ACCESSION   DAONUO010000325 DAONUO010000000
VERSION     DAONUO010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41640_length_1859_cov_2.453991"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PK768_09745"
     CDS             <1..685
                     /locus_tag="PK768_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQA61295.1"
                     /translation="TACGIEMIHTYSLIHDDLPVMDNDDFRRGKPTCHKVYGEALALL
                     AGDALLTHAFATIAMNSKIIGISLKAVLDVIEKVSLAAGGYGMIGGQVIDIETSGKNI
                     DRDTLYYISNHKTGYLIKISLWSGARLAGASKEDLEIIELFGEKIGLAFQIVDDMLDE
                     KGDENTLGKPVGSDIKNLKNTFVSMYGNIGCVKIINELSLEAKKLISHFKNSDFFIQL
                     TDFIVNRSY"
     gene            753..908
                     /locus_tag="PK768_09750"
     CDS             753..908
                     /locus_tag="PK768_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61296.1"
                     /translation="MVQYSSQNPSSEGGAKNPSKGTSMKFLVLASDAQSGAGAGSKED
                     GAIYKGM"
     gene            1369..>1859
                     /locus_tag="PK768_09755"
     CDS             1369..>1859
                     /locus_tag="PK768_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61297.1"
                     /translation="MNSDIEKRKHKKNNGGSKANWVHKVVILAFGLSVFFSTISEILL
                     KQVNLIISMFILLFIIIIGVFFDIIGVAVTSSNEAPFHAMAADKVPGSKEAVRLVRNA
                     DIVSNFCNDVVGDICGIVSGAAGAAIVVRVAATGFSQNQVIIFSILLTGIISTLTIGG
                     KALG"
BASE COUNT          553 a          316 c          446 g          544 t
ORIGIN      
        1 aactgcttgc ggcatcgaaa tgatacatac atattcgctg attcacgatg acttgcctgt
       61 tatggataat gatgattttc gcaggggaaa gcccacgtgc cacaaagtat acggtgaggc
      121 attagcccta cttgctggag acgccctact gacacatgct tttgctacaa tagcaatgaa
      181 cagcaaaatc attggtatca gtcttaaagc tgtcttggat gtaatcgaaa aagtatctct
      241 ggccgccgga ggatatggaa tgattggtgg acaagtgatt gacatcgaaa catccggaaa
      301 gaatatagac agggataccc tatattacat aagcaatcat aagacgggat atttgataaa
      361 gatttcactc tggtcggggg ctcgacttgc tggagcatct aaggaagacc tggaaataat
      421 agagcttttt ggagaaaaga taggcttggc ttttcaaatc gtcgatgata tgttagatga
      481 aaaaggtgac gaaaacactt tgggcaaacc tgtgggcagt gatattaaaa atttgaaaaa
      541 cacctttgtt agtatgtacg gtaatattgg ttgtgtaaaa ataattaacg aattgtcttt
      601 ggaagccaag aaattaatat cacattttaa aaacagcgac ttttttatac agttaacaga
      661 ttttattgta aaccgtagct attgaccaac tttccacttc cgttgaatca gtttcgtatt
      721 tgtgttataa tatttttcag caagggaaac aggtggtaca gtattctagt caaaatccct
      781 cctctgaggg cggggctaaa aatccgtcaa agggcacatc gatgaagttc ctagtgctgg
      841 cttctgacgc ccagtcgggg gctggtgctg ggagtaagga agatggggcg atctacaaag
      901 gcatgtaggc gttgacccta cttccgcgga gacctaagtt cgtggcctga taggactcct
      961 tgagggctat ggcttagggt gtgaacctgc attcgatgaa aggttgggtg cagtgtagcc
     1021 tgccttgagt gaaatgggcg gaaaaaggtt tttgttgatt tgtaatttgg ccaattacaa
     1081 atcaattttt tacctttgaa atccattttg caaaagaggc taggaggaga aggattttgg
     1141 tgaggaaatc tcctagactg gtccaatatt ggagatggat aggggattac agtgtggact
     1201 aagtggtaat ctagctctgt ttttggcgac ataacagcag tagaattaaa ggggaaccgt
     1261 ctattcggca acgaatagat ttctgctggg gaaatcctgc tagacctaag ccacaaaatt
     1321 aacccatcca ttaccatctg tttcccttaa gaaactggag gtattttgtt gaactctgat
     1381 atagaaaaga ggaagcataa aaaaaataac ggaggttcaa aagccaattg ggtacacaag
     1441 gtcgttattc tggcttttgg gctttccgtt ttttttagta cgatttcgga aatcttatta
     1501 aagcaagtta atcttataat ttcgatgttc atattgctgt ttataataat aattggtgtt
     1561 ttctttgata ttataggagt agctgttact tcatccaatg aagcaccttt tcacgccatg
     1621 gcggcagata aggttccggg aagcaaggaa gcagtcagac tcgttagaaa tgctgacata
     1681 gtttcaaact tttgcaatga cgttgtggga gacatatgtg gtattgtaag cggtgcagca
     1741 ggtgcagcca tagttgtcag agttgccgca acaggtttta gccaaaatca ggtaattatt
     1801 tttagcatac ttttaaccgg tattatttct accttaacca tcggaggtaa agcattagg
//
LOCUS       DAONUO010000326         1825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_42665_length_1825_cov_3.371186, whole genome shotgun sequence.
ACCESSION   DAONUO010000326 DAONUO010000000
VERSION     DAONUO010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42665_length_1825_cov_3.371186"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK768_09760"
     CDS             <1..386
                     /locus_tag="PK768_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQA61298.1"
                     /translation="VIESCKIVRGVIRNIMLGAVDITQDPKVQERKNQLIEEAQITLA
                     AIKSLGCDVDDPLADPATLARAAKLGILDAPHLKGNEGAPGTLVTRLIDGALYAYDEK
                     EKRILSEKERIDRILSENKIGHTVM"
     gene            526..960
                     /locus_tag="PK768_09765"
     CDS             526..960
                     /locus_tag="PK768_09765"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQA61299.1"
                     /translation="MAMDKTGDAIVLNRILESAVQAPFNKKLDIKIVKVQRGSVELEL
                     DTNPDLANGIGIVHGGVTASLCDSAMGFAVMTLGFFPLTVEMKLNYLLPGRIGDRLIA
                     KGKIIKEGKSLMIVEGEIYSGEKLIVKGIGTYLAQKPKKEIE"
     gene            991..1797
                     /locus_tag="PK768_09770"
     CDS             991..1797
                     /locus_tag="PK768_09770"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQA61300.1"
                     /translation="MLLDLNPIAFKLGPFSVHWYGIFVALSFLVGSYYLLKMGKKKGL
                     DEDFILNLAMIVIVSGIIGARLVFVLSNYPEWFIKEPLNVIKVWEGGLAWHGGLLGGL
                     LVGWNYCKRHGVNPNVLADWAVVGLCFGYIIVRIGNIFNQEVLGRMTEFAFGRWPAQL
                     VGSSIGVILLIRYFYLQGKGVPTGYQFWSFIWYHQLLRALIEETVRENPLFIIKYINP
                     HWGIGLVTLTQWFTPLVMGIAYYMYKTTGGDSMKSVDLSKYRIYDDGNWS"
BASE COUNT          530 a          354 c          430 g          511 t
ORIGIN      
        1 atgtaattga aagctgcaag atagtgcggg gtgtaatccg caatatcatg ctcggagccg
       61 tagacattac tcaagatcca aaggttcaag agcggaaaaa tcagctcatt gaggaagccc
      121 aaattaccct ggctgcaata aaatccctgg gttgcgatgt ggatgatccc ttagccgatc
      181 ctgctaccct ggcccgtgct gccaagctgg gaatcctgga tgcaccgcat cttaaaggaa
      241 atgaaggagc tcccggaacc ttggtcacac gcttaataga tggggcgcta tatgcttacg
      301 atgaaaaaga aaaacggata ctttcggaaa aagagcgcat cgacaggata cttagcgaaa
      361 acaaaatagg tcataccgta atgtaaaacc ggcgaaaatg ccggttttgt ttaatttttt
      421 tgccaccatt tctaatcttt tacttgtttt tcaaataccg ttctgatacc atatttaaaa
      481 gaaaaataca atacctcata ttttgtttat attggaggct ggattatggc tatggacaaa
      541 accggagatg ctatagtgct caatagaata ttggaaagtg cagttcaagc tccctttaat
      601 aagaagctgg atattaaaat cgtaaaagtg caaaggggca gtgttgaatt ggaactagat
      661 acgaatcctg atttggcaaa tggcatcggg attgttcatg gtggcgtcac tgcaagtctt
      721 tgcgactcag ccatgggctt tgctgttatg acacttggct tttttccttt aacggttgag
      781 atgaaactta actatttgtt gcccggccgc attggggaca gattaattgc caagggcaag
      841 atcatcaagg aaggcaaaag cttaatgata gttgagggag aaatttacag cggggaaaag
      901 ttaattgtca agggcattgg aacttatctc gcgcaaaaac ccaaaaaaga aattgagtaa
      961 ctaataaacc aatcgaatgg aggtcattaa atgcttctcg atttaaaccc tattgccttt
     1021 aaactgggcc ccttttcagt acactggtac ggcatatttg tagccctttc cttcctagtt
     1081 ggttcctact atctcttaaa aatggggaaa aagaagggcc ttgatgaaga tttcatatta
     1141 aatcttgcca tgatagtcat tgtatccggc ataattggag cccgtcttgt attcgtgctc
     1201 agcaattatc ccgaatggtt tataaaagaa ccactcaatg taataaaggt atgggaaggg
     1261 ggcttagcct ggcatggcgg tctccttggc ggccttctgg taggctggaa ttactgtaaa
     1321 agacatggag taaatcccaa cgtcctcgcc gattgggctg ttgttggttt gtgttttgga
     1381 tatattatcg tgcggatagg caatattttc aaccaggaag ttttggggcg aatgacggag
     1441 tttgccttcg gaagatggcc agctcagctt gttgggtctt caataggagt aatactgctt
     1501 attagatatt tttaccttca aggaaaaggt gtccctacgg gctaccagtt ttggtctttc
     1561 atctggtacc accagctgct gagggccctt atagaagaga ccgtacggga aaatcccctg
     1621 ttcatcataa aatacatcaa tcctcactgg ggcataggcc tggttacgct gacccagtgg
     1681 tttacacctt tagtaatggg cattgcctat tatatgtata agaccaccgg cggagacagc
     1741 atgaaaagtg tggacctttc taaataccgc atttacgatg atgggaattg gagctaaaca
     1801 ttgacttaga gccaaatatg agtta
//
LOCUS       DAONUO010000327         1777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_44155_length_1777_cov_2.583624, whole genome shotgun sequence.
ACCESSION   DAONUO010000327 DAONUO010000000
VERSION     DAONUO010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44155_length_1777_cov_2.583624"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..995
                     /locus_tag="PK768_09775"
     CDS             159..995
                     /locus_tag="PK768_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQA61301.1"
                     /translation="MTRKIISIVSLILVALLVVSCGSAQNSQPQAENSEDGQPIVLKV
                     GASPVPHAEILNVVKPLLEAEGIELQIVEFADYNQPNLRLADKDLDANYFQHIPYLES
                     FSNDHNLDLTYTAKVHIEPMGIYSEKIKDISELEDGAEIAIPNDPTNGGRALILLEQA
                     GLLKIKEGVGITATVYDIVENPKNLKITELEAATLPRVLGDVAAAVINSNYALEAKFV
                     PTKDALVLESPQDNPYVNILAIRKGDENRPEIVKLSEALNSPEVKKFIEDEYQGAVIP
                     AF"
     gene            1113..1775
                     /locus_tag="PK768_09780"
     CDS             1113..1775
                     /locus_tag="PK768_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HQA61302.1"
                     /translation="MACTIKIGVIAGTPVDTQMGVDFVKRQGFEALGYASASSPEEQN
                     RLQFLEPEFLTQKVLDIIGDFESQKIFGTMIYCNSLSAAIDIEHIRRARPKSTVVTPL
                     DVYRNLALKYKKIALWAANGQCLSTIEHIFYEQKPSIDIMGVSMLPVIKAIENGEAPK
                     AIVEKFDLVSLGLECFDAECLILGCTHLPYLQRELSKGLSIPIIDPADEMLRLLTKSC
                     QA"
BASE COUNT          523 a          401 c          399 g          454 t
ORIGIN      
        1 ttggatacaa ccggttccgc cccgacatca tgctttacac cgtggccatc ctcgtggtaa
       61 tggtccagct catacagatg gctggagatg ctgttgttaa gcatctatcc aaaaaccgct
      121 aattaaaaat cactttgaaa acttaaggag gaaatcagat gacaagaaaa ataatttcca
      181 ttgtttcatt gatattagtt gccttgcttg ttgtgagctg cggttctgct cagaattcac
      241 aacctcaagc tgaaaattcc gaagacggtc agcctattgt cctgaaagta ggggcatccc
      301 ctgtccccca tgcagaaatc ttaaatgttg taaagcccct tttggaagca gaaggaattg
      361 agctgcagat agtggaattt gccgactaca atcagcctaa tttaaggctt gcagataagg
      421 acttggatgc caactacttc cagcacattc cttatttaga gagcttttct aatgaccaca
      481 atttagacct gacttatacc gcaaaggtgc acatagagcc catgggcata tattctgaaa
      541 agattaaaga catcagcgag ctcgaagatg gtgcggagat tgcaatacca aatgacccca
      601 caaacggcgg aagggcactc attctgctgg agcaggccgg actcttaaaa atcaaagagg
      661 gcgtgggcat aactgctaca gtttacgaca tcgtagagaa ccccaaaaac ctaaagatta
      721 ccgaacttga agctgccacc ctgccaagag tcctggggga tgtggctgct gccgtcatca
      781 actccaatta tgcactggaa gccaaattcg tcccaacaaa agacgccttg gttctcgaat
      841 cgccacagga taacccttat gtgaatattt tggccatccg caagggcgat gaaaacaggc
      901 ctgaaatagt aaaactttcc gaggccttaa actctccgga agttaagaaa ttcattgaag
      961 acgagtatca gggtgccgta ataccggctt tttaatagag taactgcggg cattagcgta
     1021 taggtgaagg tgaaaagact attctttcgc cttcacctat ttattaacac ttgcgatata
     1081 ataaatatta gcaaatagat ttaggtgatt ttatggcttg cacaataaaa attggcgtca
     1141 ttgccgggac gcctgttgac acacagatgg gtgttgactt cgtgaaaaga cagggtttcg
     1201 aagctcttgg ctatgcatcc gcctcaagtc ccgaagagca aaaccgcctt cagtttttag
     1261 agcctgagtt cctcacacaa aaagttttgg atatcatcgg ggattttgaa agtcagaaga
     1321 tattcggcac catgatttac tgcaattcgc tgtcggctgc aatagatatc gagcacatac
     1381 gaagggcacg gccgaagtct actgttgtaa ctcctcttga cgtttacaga aacctggcat
     1441 tgaagtacaa aaaaattgcc ctttgggctg caaacggtca atgcctttcg acaatcgagc
     1501 acatatttta tgaacaaaag ccatccatcg acattatggg tgtatccatg ctaccggtca
     1561 taaaggctat cgagaacggg gaagctccga aagccatcgt tgaaaaattt gacctggtat
     1621 cgctgggatt ggagtgtttt gacgccgaat gtcttattct tggctgcacc cacctgccct
     1681 atcttcagcg ggagctatcc aaagggctca gcatccccat aatcgatccg gcagatgaga
     1741 tgttaaggct cttaacaaag tcttgtcaag cttaagg
//
LOCUS       DAONUO010000328         1743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_45326_length_1743_cov_2.845972, whole genome shotgun sequence.
ACCESSION   DAONUO010000328 DAONUO010000000
VERSION     DAONUO010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45326_length_1743_cov_2.845972"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PK768_09785"
     CDS             complement(<1..812)
                     /locus_tag="PK768_09785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA61303.1"
                     /translation="MIKRLYECIRSLKINCLAFLAVLPLFAFTFIFVIGPLAYMLILS
                     FLQRAEVWGVVNKFTFKNYRDILEPVYLKTFIESFKLAITTTFLITIIGYPFGYFMAK
                     LDAKWKKRMMMFIFVPFWTSSLIRLYGWIIIFRANGVFDRFLMTLNITDAPLKLLYSY
                     PAVLVGMVYVLLPFMILAVYSSAEKLDWSLVEAARDLGASSFQAFLTISLKLTLPGLL
                     SGVVLTFIPSMGLFFIADILGVNKVVLVGNLIQEQLMKAHNWPFAAALSVIL"
     gene            complement(809..>1743)
                     /locus_tag="PK768_09790"
     CDS             complement(809..>1743)
                     /locus_tag="PK768_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA61304.1"
                     /translation="RLEKPDKGKVYLEGKDVTNAEPNRRNVNTVFQNYALFPHMTVEG
                     NISYSLRLKRLDRARIKETVSKALELVQLVGFEKRMPSELSGGQRQRVAIARAIVNEP
                     RVLLLDEPLGALDLQLRRQMQTELKRLQKHLGITFVYITHDQEEALNMSDRIAVMRDG
                     KFEQIGTPQEVYDQPKTSFVASFVGSANIIYGTVSHVNEDTLVFENKNGIGIAARNKI
                     NILKGQKITVAVRTEHISLSRISSKGNATGLMGVVKEKSFAGGLLQIFVNLADGQEII
                     ASRHGIDSLLVPGDNVIVTWAPEKAILVDLEGAQ"
BASE COUNT          500 a          406 c          368 g          469 t
ORIGIN      
        1 atcaggatca cggaaagggc agcagcaaat ggccagttgt gagctttcat gagctgctcc
       61 tgaatgagat tgccaactag aactacttta ttcacaccta aaatatcggc aataaaaaac
      121 aggcccattg atggaataaa ggtcagcact acacccgaca aaagtcccgg caaggtcaat
      181 tttaatgaaa tggttaaaaa tgcttgaaag gaacttgctc ccaaatctcg tgccgcttcc
      241 accaagctcc agtcaagttt ttcagcgctg gaatatacag caagaatcat aaagggcaaa
      301 aggacgtaca ccatccctac cagcactgca gggtagctgt ataaaagctt taaaggcgcg
      361 tcggtaatat ttagtgtcat caggaatctg tcgaaaacac cattggcgcg aaaaatgatg
      421 atccatccat agagcctgat gagggaactg gtccaaaatg gtacgaatat aaacatcatc
      481 atgcgctttt tccatttcgc atctaatttc gccataaaat agccaaaggg atatccgata
      541 atcgtgataa gaaaagtagt agtaattgca agtttaaatg actctataaa tgtctttaaa
      601 tatacaggtt ccaagatatc tctgtagttt ttaaaggtga acttatttac cactccccag
      661 acttcagccc gctgcaaaaa gctaagtatc agcatatagg ccaagggacc tataacaaag
      721 ataaatgtga aagcaaaaag gggcaagacg gcaaggaaag ccaggcagtt tattttaagg
      781 gagcgaatac attcgtacag tctctttatc attgcgcacc ctccaagtca actaggatag
      841 ccttttccgg tgcccatgtt acaatcacat tatctcccgg gacaagaaga gaatcaatac
      901 cgtgacggct agctatgatc tcttgaccat ctgctaaatt tacaaagatc tgcaacaggc
      961 ctccggcaaa gctcttttcc ttgaccactc ccataaggcc agttgcattg ccctttgaag
     1021 atattcggct taagctgatg tgctcggttc gaacagctac ggttatcttt tggcccttaa
     1081 ggatatttat cttgtttctt gcggcaatac cgatgccgtt tttattttca aagacgaggg
     1141 tatcttcatt cacatgggat actgtgccgt aaatgatatt ggcagaaccg acaaaactgg
     1201 ccacgaagct tgttttgggc tggtcgtaaa cttcttgagg tgtgccaatc tgttcaaatt
     1261 tgccatcccg catgacagca atcctatcgg acatattgag ggcttcttcc tggtcatggg
     1321 tgatatatac gaaggttata cccaagtgct tctgcagtcg cttaagctct gtctgcatct
     1381 gacgcctaag ttgcaagtct agtgccccaa gaggctcatc caataatagg accctgggct
     1441 cgttcactat ggcccgggct atggccaccc gctggcgctg acctccagaa agttcgctgg
     1501 gcatcctctt ttcaaaacct accagttgaa caagctctaa agccttagaa accgtctctt
     1561 ttatccttgc cctgtcaagg cgtttaaggc gcaggctgta gcttatattg ccttcaacgg
     1621 tcatatgggg aaaaagggcg taattttgaa agaccgtatt cacatttcgc ctatttggtt
     1681 ctgcgtttgt aacatccttt ccttccaagt aaacctttcc cttatcgggc ttttcaaggc
     1741 ggc
//
LOCUS       DAONUO010000329         1548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_53357_length_1548_cov_3.705961, whole genome shotgun sequence.
ACCESSION   DAONUO010000329 DAONUO010000000
VERSION     DAONUO010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53357_length_1548_cov_3.705961"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..921)
                     /locus_tag="PK768_09795"
     CDS             complement(133..921)
                     /locus_tag="PK768_09795"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA61305.1"
                     /translation="MIDIHSHILPGLDDGSPDMDTSLKMVEIAASEGINTIVATPHFI
                     PNDLELDRNLVLEKVEELNRCIKQHTDKDVKIYPGEEVFLSPEIPELYSEGKLITLFD
                     QGKYLLIELPMVTIPPYTKEVIYSLNLKGLKPIIAHPERNREIARNVDKLRELISMGA
                     LAQVNSLSFYGVFGRNAKIAAEKIVKKGLAQMIATDSHTARARSPRFQKLYKSFSPKL
                     IDIMMRQNPEKIMAGLDIEVSSEGYKKAAKMSMFQKISFFFSHN"
     gene            complement(1044..>1548)
                     /locus_tag="PK768_09800"
     CDS             complement(1044..>1548)
                     /locus_tag="PK768_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HQA61306.1"
                     /translation="LQIKALSRKHRCKNYYKKLDNLIFASDFTVDADNDGVPDLWRIS
                     NSKDGLLEVYIGEAAKITNKGEGPSYLVTRQDFSLNPQTSYTINFFVGGDMPGEKIQF
                     YVFSREGENTQHHWKDVQLSSQISNISTSFVTTEDLVEGKQWLRIDIQGASEEPLNVK
                     SIEIWEE"
BASE COUNT          391 a          329 c          306 g          522 t
ORIGIN      
        1 cattattttc aatgggtttc aggctttttg tcttctgtaa gtgtcaaaga tgagagtata
       61 aataccaggg ctaaattttt tttagtttta aataccatgt agcacctcct tcgcatttca
      121 tgtgacagca aattaattgt ggctgaagaa aaaggatatt ttttggaaca tgctcatttt
      181 ggcagctttt ttatagcctt ccgaagatac ttctatatca agtcctgcca taattttttc
      241 cgggttctgc cgcatcatga tgtctattag ttttggtgag aaactcttgt acagtttttg
      301 aaatcgcggt gacctggcac gagcagtatg gctgtcggtg gctatcatct gggccaagcc
      361 ctttttgact attttttcgg cggcgatttt cgcgttcctg ccgaaaactc cataaaagct
      421 taaggagttg acctgagcca aagcccccat cgatataagt tctctaagct tgtccacgtt
      481 tcgggcaatc tcccgattgc gttccggatg ggctatgatg ggctttaacc cttttaagtt
      541 caatgagtat ataacttctt ttgtgtaggg tgggatggtt accatgggta attctatgag
      601 caggtatttg ccctgatcaa agagggtgat aagctttcct tcgctgtaga gctcaggtat
      661 ttccggcgaa agaaaaactt cttcacccgg gtatatcttt acgtccttat cggtatgttg
      721 ttttatacac cggtttaatt cctctacttt ttccagtacc aggtttctat ctaactcaag
      781 atcatttggg ataaaatggg gtgtggccac aatggtgttt atgccttcgg aagccgctat
      841 ttcgaccatt tttaaagatg tatccatgtc cggagagccg tcatccaatc cgggcagaat
      901 gtgactgtga atatctatca ttattttgcc tcctagtcat gttgttcgcc ctatttctcc
      961 tgcccaagac gaaatcttta tcatctacta aaattatgca ttctttcaca aaaaatgtca
     1021 acctttgtcc gaaaaatccg ctctcattct tcccatattt caattgattt cacattcaat
     1081 ggctcctcag aagctccctg aatatctatt cgcaaccact gcttgccttc tacaaggtcc
     1141 tccgtagtaa caaaggatgt gctgatattc gaaatctggc tggaaagttg gacgtccttc
     1201 caatggtgct gagtattttc accttcccga gaaaaaacgt aaaattggat tttttcccct
     1261 ggcatatctc caccgacaaa aaagtttata gtatatgagg tttggggatt tagggaaaaa
     1321 tcttgccttg taacaagata agatggtccc tcacccttat ttgttatttt agcagcttct
     1381 cctatataaa cttccaatag cccgtccttg gaattggata ttctccaaag atccggcaca
     1441 ccatcattgt ctgcatcaac cgtaaagtcc gatgcaaaga tgaggttatc aagtttctta
     1501 tagtaatttt tgcatcgatg ttttcggcta agagctttga tttgtaga
//
LOCUS       DAONUO010000330         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tepidanaerobacteraceae bacterium isolate AMR_MDS_4224
            NODE_55530_length_1505_cov_3.620000, whole genome shotgun sequence.
ACCESSION   DAONUO010000330 DAONUO010000000
VERSION     DAONUO010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866195
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tepidanaerobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Tepidanaerobacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia;
            Thermosediminibacterales; Tepidanaerobacteraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 04:46:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,962
            CDSs (total)                      :: 1,930
            Genes (coding)                    :: 1,922
            CDSs (with protein)               :: 1,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 5 of 8
            Pseudo Genes (internal stop)      :: 2 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Tepidanaerobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55530_length_1505_cov_3.620000"
                     /isolate="AMR_MDS_4224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2963290"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK768_09805"
     CDS             <1..364
                     /locus_tag="PK768_09805"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQA61307.1"
                     /translation="TDEQIRALAKTGGVIGINAVSTLVAERNADVKALVDHVDHLRDV
                     AGIHHIGLGLDFCDKIFELPNPRKNPKSKGLYDVIEGHKNIYQLTEELINRGYGDDEI
                     RLIYGENFLRVFKEVILK"
     regulatory      444..529
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            768..>1505
                     /locus_tag="PK768_09810"
     CDS             768..>1505
                     /locus_tag="PK768_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HQA61308.1"
                     /translation="MKEGVNYNYLDSFIDVEESLIENKETDSYAYIKNGCLKVHHPPE
                     GPYPIVIPCPGVKLVINGRQYTEPMPIFMEDEVSIHTEDELIEGHWSLSVSEDGLEAI
                     LHIEPTVIIHRKLLDLPPARILELEVEESREYRCPITLEELLRELTAKKISYGIDWQN
                     CTKALKTCKTDKIVIARGMPAKKGKDGRVELLFKPDLKTAIVSDEDETVDFRKRYIFT
                     SIEAGEVLAVKHPPEIGSSGRSVTGQII"
BASE COUNT          521 a          266 c          329 g          389 t
ORIGIN      
        1 cacggatgag cagataagag ccctggccaa aaccggcggt gtcatcggca taaatgccgt
       61 aagcactttg gttgcagaaa gaaatgccga tgtcaaagca ttagtggatc acgtggacca
      121 cttgcgggat gtggcaggca tccatcacat aggtcttggt ttagactttt gcgataaaat
      181 ttttgagctc ccaaatccca gaaagaaccc aaaatcaaaa gggctttacg atgtaataga
      241 gggtcataaa aacatatacc aactcacgga agaattgatt aatagaggat atggcgatga
      301 tgaaatccgc cttatttacg gggaaaattt tttaagagta tttaaagaag tcatattaaa
      361 ataaaacttt tttcacagga aggcatgaca aaagtcctat tttatagtat aatagtattg
      421 taaaatgtga gacaagcaaa tatttgtgat aagggcaaac catttgaaaa aatgggacgc
      481 aaagccatgg gtctgaagcc aaatgaggta tgattgccag gctgccacaa taataaaaat
      541 gaatgatata tcaattatgt gagttcataa ttaattacta tattattatg gcagctgttg
      601 gctgccattt ttcttgccta tcaacaatct cttcagaaca tagtgaaatc ttcatgcttt
      661 atcgagccta ttacgaaaca gcaagctatc ctgaaccgat aagactttca cattgagtcc
      721 tcaggtctag ttctcattga caacatctga aaggcggaga ttgcgaaatg aaggaagggg
      781 taaattacaa ttacttggat tcatttattg atgtcgaaga aagccttatt gaaaataaag
      841 agacagattc ttatgcatat attaaaaacg gatgcctgaa agtacatcat cctccagagg
      901 gcccgtatcc catagtaatt ccttgtcccg gagtaaaact ggttatcaac ggaaggcaat
      961 acaccgaacc tatgccaatt tttatggaag atgaggtatc gatacatacg gaagatgaat
     1021 taatagaagg tcactggtct ttgtctgtat ctgaggatgg tttagaagcg atattgcata
     1081 tagagcctac ggtaataata cataggaaat tgttagacct tcctcctgcc aggatactgg
     1141 aattagaagt agaagaatcg agagaataca gatgcccgat aacattggaa gagctgctcc
     1201 gagaattaac tgcaaaaaag atcagctacg gcatagattg gcaaaactgc actaaagcat
     1261 taaagacttg caaaacagat aaaatagtca ttgcaagagg catgccagct aaaaagggaa
     1321 aagacgggcg cgtagaactg ctttttaaac ctgatcttaa gacagccata gtatctgatg
     1381 aagatgaaac ggtggatttt cgcaagcgct atatctttac ttctatagag gccggtgagg
     1441 ttcttgcagt aaagcaccca cccgaaattg gcagcagcgg cagaagtgtt acgggccaaa
     1501 taata
//
